LOCUS       DACDSY010000001       114639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDSY010000001 DACDSY010000000
VERSION     DACDSY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 114639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 114639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..114639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.001"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(295..3006)
                     /locus_tag="IWK44_000001"
     CDS             complement(295..3006)
                     /locus_tag="IWK44_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9548560.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(3016..3717)
                     /locus_tag="IWK44_000002"
     CDS             complement(3016..3717)
                     /locus_tag="IWK44_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9548561.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            3903..4502
                     /locus_tag="IWK44_000003"
     CDS             3903..4502
                     /locus_tag="IWK44_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9548562.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            4583..5596
                     /locus_tag="IWK44_000004"
     CDS             4583..5596
                     /locus_tag="IWK44_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9548563.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            5704..6621
                     /gene="rbsK"
                     /locus_tag="IWK44_000005"
     CDS             5704..6621
                     /gene="rbsK"
                     /locus_tag="IWK44_000005"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP9548564.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            6649..7044
                     /gene="rbsD"
                     /locus_tag="IWK44_000006"
     CDS             6649..7044
                     /gene="rbsD"
                     /locus_tag="IWK44_000006"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP9548565.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            7044..8417
                     /locus_tag="IWK44_000007"
     CDS             7044..8417
                     /locus_tag="IWK44_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9548566.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            8774..10918
                     /locus_tag="IWK44_000008"
     CDS             8774..10918
                     /locus_tag="IWK44_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9548567.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(10970..12010)
                     /locus_tag="IWK44_000009"
     CDS             complement(10970..12010)
                     /locus_tag="IWK44_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9548568.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(12287..14089)
                     /locus_tag="IWK44_000010"
     CDS             complement(12287..14089)
                     /locus_tag="IWK44_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9548569.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(14103..14855)
                     /locus_tag="IWK44_000011"
     CDS             complement(14103..14855)
                     /locus_tag="IWK44_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9548570.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(14864..15226)
                     /locus_tag="IWK44_000012"
     CDS             complement(14864..15226)
                     /locus_tag="IWK44_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9548571.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(15402..16274)
                     /locus_tag="IWK44_000013"
     CDS             complement(15402..16274)
                     /locus_tag="IWK44_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP9548572.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(16300..16812)
                     /locus_tag="IWK44_000014"
     CDS             complement(16300..16812)
                     /locus_tag="IWK44_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP9548573.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(16950..17780)
                     /locus_tag="IWK44_000015"
     CDS             complement(16950..17780)
                     /locus_tag="IWK44_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP9548574.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(17796..18404)
                     /locus_tag="IWK44_000016"
     CDS             complement(17796..18404)
                     /locus_tag="IWK44_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP9548575.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(18397..18714)
                     /locus_tag="IWK44_000017"
     CDS             complement(18397..18714)
                     /locus_tag="IWK44_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9548576.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(18870..19598)
                     /locus_tag="IWK44_000018"
     CDS             complement(18870..19598)
                     /locus_tag="IWK44_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP9548577.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            19757..20638
                     /locus_tag="IWK44_000019"
     CDS             19757..20638
                     /locus_tag="IWK44_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9548578.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(20875..21918)
                     /locus_tag="IWK44_000020"
     CDS             complement(20875..21918)
                     /locus_tag="IWK44_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9548579.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(21921..22589)
                     /locus_tag="IWK44_000021"
     CDS             complement(21921..22589)
                     /locus_tag="IWK44_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9548580.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            22731..25067
                     /locus_tag="IWK44_000022"
     CDS             22731..25067
                     /locus_tag="IWK44_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9548581.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(25182..25493)
                     /locus_tag="IWK44_000023"
     CDS             complement(25182..25493)
                     /locus_tag="IWK44_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548582.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            25674..27149
                     /locus_tag="IWK44_000024"
     CDS             25674..27149
                     /locus_tag="IWK44_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP9548583.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            27196..27780
                     /locus_tag="IWK44_000025"
     CDS             27196..27780
                     /locus_tag="IWK44_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP9548584.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            27782..28201
                     /locus_tag="IWK44_000026"
     CDS             27782..28201
                     /locus_tag="IWK44_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP9548585.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(28482..28766)
                     /locus_tag="IWK44_000027"
     CDS             complement(28482..28766)
                     /locus_tag="IWK44_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP9548586.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(29118..29828)
                     /locus_tag="IWK44_000028"
     CDS             complement(29118..29828)
                     /locus_tag="IWK44_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP9548587.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(29825..31297)
                     /locus_tag="IWK44_000029"
     CDS             complement(29825..31297)
                     /locus_tag="IWK44_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9548588.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            31435..31908
                     /locus_tag="IWK44_000030"
     CDS             31435..31908
                     /locus_tag="IWK44_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9548589.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(32061..32612)
                     /locus_tag="IWK44_000031"
     CDS             complement(32061..32612)
                     /locus_tag="IWK44_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9548590.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            32717..33247
                     /locus_tag="IWK44_000032"
     CDS             32717..33247
                     /locus_tag="IWK44_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP9548591.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(33730..34173)
                     /locus_tag="IWK44_000033"
     CDS             complement(33730..34173)
                     /locus_tag="IWK44_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9548592.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(34189..35349)
                     /locus_tag="IWK44_000034"
     CDS             complement(34189..35349)
                     /locus_tag="IWK44_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9548593.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQERIYFFEGRKLVKELQKNGFYFKINE"
     gene            35856..37556
                     /locus_tag="IWK44_000035"
     CDS             35856..37556
                     /locus_tag="IWK44_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548594.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(37658..38491)
                     /locus_tag="IWK44_000036"
     CDS             complement(37658..38491)
                     /locus_tag="IWK44_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP9548595.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(38795..40960)
                     /locus_tag="IWK44_000037"
     CDS             complement(38795..40960)
                     /locus_tag="IWK44_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9548596.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            41220..43514
                     /locus_tag="IWK44_000038"
     CDS             41220..43514
                     /locus_tag="IWK44_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9548597.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            43507..44172
                     /gene="pgmB"
                     /locus_tag="IWK44_000039"
     CDS             43507..44172
                     /gene="pgmB"
                     /locus_tag="IWK44_000039"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9548598.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            44165..45172
                     /locus_tag="IWK44_000040"
     CDS             44165..45172
                     /locus_tag="IWK44_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9548599.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            45194..46213
                     /locus_tag="IWK44_000041"
     CDS             45194..46213
                     /locus_tag="IWK44_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9548600.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(46494..47861)
                     /gene="nhaC"
                     /locus_tag="IWK44_000042"
     CDS             complement(46494..47861)
                     /gene="nhaC"
                     /locus_tag="IWK44_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9548601.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(47971..49392)
                     /locus_tag="IWK44_000043"
     CDS             complement(47971..49392)
                     /locus_tag="IWK44_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9548602.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(49635..51512)
                     /gene="tdc"
                     /locus_tag="IWK44_000044"
     CDS             complement(49635..51512)
                     /gene="tdc"
                     /locus_tag="IWK44_000044"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9548603.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(51803..53059)
                     /locus_tag="IWK44_000045"
     CDS             complement(51803..53059)
                     /locus_tag="IWK44_000045"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP9548604.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            53639..54940
                     /locus_tag="IWK44_000046"
     CDS             53639..54940
                     /locus_tag="IWK44_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9548605.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(55009..55608)
                     /locus_tag="IWK44_000047"
     CDS             complement(55009..55608)
                     /locus_tag="IWK44_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9548606.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            55862..56743
                     /locus_tag="IWK44_000048"
     CDS             55862..56743
                     /locus_tag="IWK44_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9548607.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            56756..58366
                     /locus_tag="IWK44_000049"
     CDS             56756..58366
                     /locus_tag="IWK44_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP9548608.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(58443..59102)
                     /locus_tag="IWK44_000050"
     CDS             complement(58443..59102)
                     /locus_tag="IWK44_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9548609.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(59385..60050)
                     /locus_tag="IWK44_000051"
     CDS             complement(59385..60050)
                     /locus_tag="IWK44_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548610.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(60197..60802)
                     /gene="dhaL"
                     /locus_tag="IWK44_000052"
     CDS             complement(60197..60802)
                     /gene="dhaL"
                     /locus_tag="IWK44_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP9548611.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(60830..61813)
                     /gene="dhaK"
                     /locus_tag="IWK44_000053"
     CDS             complement(60830..61813)
                     /gene="dhaK"
                     /locus_tag="IWK44_000053"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP9548612.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(61815..62210)
                     /gene="dhaM"
                     /locus_tag="IWK44_000054"
     CDS             complement(61815..62210)
                     /gene="dhaM"
                     /locus_tag="IWK44_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP9548613.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            62364..62684
                     /locus_tag="IWK44_000055"
     CDS             62364..62684
                     /locus_tag="IWK44_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP9548614.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(62796..63665)
                     /locus_tag="IWK44_000056"
     CDS             complement(62796..63665)
                     /locus_tag="IWK44_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9548615.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(63761..65035)
                     /locus_tag="IWK44_000057"
     CDS             complement(63761..65035)
                     /locus_tag="IWK44_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9548616.1"
                     /translation="MLRYLFVYYVFFLVSYPILGGFVWFIGVWCYVFLYKHKQKEWVD
                     VPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGSTDQTPEILARL
                     MKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDALIRYVNYFIRP
                     GARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLYAYSGANTMYR
                     KEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTLKMLYRQRKRW
                     AKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVLFAGLIGHYVY
                     QGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFAPLYMLLFWIV
                     NAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(65061..65570)
                     /locus_tag="IWK44_000058"
     CDS             complement(65061..65570)
                     /locus_tag="IWK44_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548617.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(65745..67880)
                     /locus_tag="IWK44_000059"
     CDS             complement(65745..67880)
                     /locus_tag="IWK44_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9548618.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(67963..69618)
                     /locus_tag="IWK44_000060"
     CDS             complement(67963..69618)
                     /locus_tag="IWK44_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9548619.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(69620..69934)
                     /locus_tag="IWK44_000061"
     CDS             complement(69620..69934)
                     /locus_tag="IWK44_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9548620.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            70104..70364
                     /locus_tag="IWK44_000062"
     CDS             70104..70364
                     /locus_tag="IWK44_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP9548621.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            70378..70692
                     /locus_tag="IWK44_000063"
     CDS             70378..70692
                     /locus_tag="IWK44_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP9548622.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(71422..71976)
                     /locus_tag="IWK44_000064"
     CDS             complement(71422..71976)
                     /locus_tag="IWK44_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP9548623.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(72102..74030)
                     /locus_tag="IWK44_000065"
     CDS             complement(72102..74030)
                     /locus_tag="IWK44_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9548624.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(74336..75052)
                     /locus_tag="IWK44_000066"
     CDS             complement(74336..75052)
                     /locus_tag="IWK44_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9548625.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(75053..76876)
                     /gene="glpO"
                     /locus_tag="IWK44_000067"
     CDS             complement(75053..76876)
                     /gene="glpO"
                     /locus_tag="IWK44_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP9548626.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(76890..78386)
                     /gene="glpK"
                     /locus_tag="IWK44_000068"
     CDS             complement(76890..78386)
                     /gene="glpK"
                     /locus_tag="IWK44_000068"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP9548627.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(78522..79982)
                     /locus_tag="IWK44_000069"
     CDS             complement(78522..79982)
                     /locus_tag="IWK44_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9548628.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(79972..81267)
                     /locus_tag="IWK44_000070"
     CDS             complement(79972..81267)
                     /locus_tag="IWK44_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9548629.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(81599..82276)
                     /locus_tag="IWK44_000071"
     CDS             complement(81599..82276)
                     /locus_tag="IWK44_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP9548630.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(82306..83397)
                     /locus_tag="IWK44_000072"
     CDS             complement(82306..83397)
                     /locus_tag="IWK44_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP9548631.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(83498..83932)
                     /locus_tag="IWK44_000073"
     CDS             complement(83498..83932)
                     /locus_tag="IWK44_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9548632.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(84516..85973)
                     /gene="ascB"
                     /locus_tag="IWK44_000074"
     CDS             complement(84516..85973)
                     /gene="ascB"
                     /locus_tag="IWK44_000074"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9548633.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(85970..87865)
                     /locus_tag="IWK44_000075"
     CDS             complement(85970..87865)
                     /locus_tag="IWK44_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9548634.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(87923..89698)
                     /locus_tag="IWK44_000076"
     CDS             complement(87923..89698)
                     /locus_tag="IWK44_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9548635.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(89748..91244)
                     /locus_tag="IWK44_000077"
     CDS             complement(89748..91244)
                     /locus_tag="IWK44_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9548636.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(91438..93060)
                     /locus_tag="IWK44_000078"
     CDS             complement(91438..93060)
                     /locus_tag="IWK44_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9548637.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(93509..94822)
                     /locus_tag="IWK44_000079"
     CDS             complement(93509..94822)
                     /locus_tag="IWK44_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9548638.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(95067..95291)
                     /locus_tag="IWK44_000080"
     CDS             complement(95067..95291)
                     /locus_tag="IWK44_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548639.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(95311..95679)
                     /locus_tag="IWK44_000081"
     CDS             complement(95311..95679)
                     /locus_tag="IWK44_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9548640.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(95877..96587)
                     /locus_tag="IWK44_000082"
     CDS             complement(95877..96587)
                     /locus_tag="IWK44_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548641.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(96605..97102)
                     /locus_tag="IWK44_000083"
     CDS             complement(96605..97102)
                     /locus_tag="IWK44_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9548642.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(97139..97591)
                     /locus_tag="IWK44_000084"
     CDS             complement(97139..97591)
                     /locus_tag="IWK44_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9548643.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(97726..98331)
                     /locus_tag="IWK44_000085"
     CDS             complement(97726..98331)
                     /locus_tag="IWK44_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9548644.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(98781..99401)
                     /locus_tag="IWK44_000086"
     CDS             complement(98781..99401)
                     /locus_tag="IWK44_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9548645.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(99403..100272)
                     /locus_tag="IWK44_000087"
     CDS             complement(99403..100272)
                     /locus_tag="IWK44_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP9548646.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(100295..100702)
                     /locus_tag="IWK44_000088"
     CDS             complement(100295..100702)
                     /locus_tag="IWK44_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9548647.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(100721..102010)
                     /locus_tag="IWK44_000089"
     CDS             complement(100721..102010)
                     /locus_tag="IWK44_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9548648.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(102037..102339)
                     /locus_tag="IWK44_000090"
     CDS             complement(102037..102339)
                     /locus_tag="IWK44_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9548649.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(102364..104457)
                     /locus_tag="IWK44_000091"
     CDS             complement(102364..104457)
                     /locus_tag="IWK44_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9548650.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(104946..105710)
                     /locus_tag="IWK44_000092"
     CDS             complement(104946..105710)
                     /locus_tag="IWK44_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP9548651.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(105821..106690)
                     /gene="mdcB"
                     /locus_tag="IWK44_000093"
     CDS             complement(105821..106690)
                     /gene="mdcB"
                     /locus_tag="IWK44_000093"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP9548652.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(106715..107791)
                     /locus_tag="IWK44_000094"
     CDS             complement(106715..107791)
                     /locus_tag="IWK44_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP9548653.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(107815..108408)
                     /locus_tag="IWK44_000095"
     CDS             complement(107815..108408)
                     /locus_tag="IWK44_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP9548654.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(108412..110088)
                     /gene="mdcD"
                     /locus_tag="IWK44_000096"
     CDS             complement(108412..110088)
                     /gene="mdcD"
                     /locus_tag="IWK44_000096"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP9548655.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(110078..110377)
                     /locus_tag="IWK44_000097"
     CDS             complement(110078..110377)
                     /locus_tag="IWK44_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP9548656.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(110479..112125)
                     /gene="mdcA"
                     /locus_tag="IWK44_000098"
     CDS             complement(110479..112125)
                     /gene="mdcA"
                     /locus_tag="IWK44_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP9548657.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(112132..113043)
                     /locus_tag="IWK44_000099"
     CDS             complement(112132..113043)
                     /locus_tag="IWK44_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9548658.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            113163..114020
                     /locus_tag="IWK44_000100"
     CDS             113163..114020
                     /locus_tag="IWK44_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9548659.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(114207..114569)
                     /locus_tag="IWK44_000101"
     CDS             complement(114207..114569)
                     /locus_tag="IWK44_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548660.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT        33579 a        23979 c        20133 g        36948 t
ORIGIN      
        1 ctttatacat tctaagaggt ctgttgccca ataaaaaaca agagatcaga catgtatttg
       61 tctatcctct tgtttaaaat agcaaaatat tcttgtttct tctataaatg gtgttcaaaa
      121 gcttttatct acggcaatca gtcttctcaa atgtaaaaca tgaggagcta attttatttg
      181 agtattcttt ttgctcgatg caggacggct tttttacaac ctctgattca tggtgttcaa
      241 ttagcagctg accctcgtta ttagctcaaa acgctgattt cacaacctct gtttttattc
      301 attcgatttc aacgcatcaa tcatatcgat atgctttaat ttgaaatggg taacaatcat
      361 cacaataacc gtaaatacga tagttaatcc tgctgataag acataagccc accatgtgat
      421 cactaatgga aagatgacat tctccataga cgcttgttgc aagatgaagt cagtcaatat
      481 atatcctaca ccaaatcctc cgatgattcc taaaagagta aagatgatat tttctcgaac
      541 aatgtacatc gtgacttctt tatcaaaaaa tccaaggact tttattgtag ataattcgcg
      601 tacacgctcg gaaatattga tattagttag attatacaag acgataaaag ccagcagtcc
      661 tgataatacg acaaagatta agacaaccga gtctaagttc gccattgatt cttcttgcgt
      721 ttcgatttgt ttggacatga aagacgtatt tgtcacagcg tctgtggcaa gcagtttctc
      781 ggaaagcatt tcttcttctt ttgttgtcat cttatctgta cgcagcaaaa atgcattatc
      841 catcatcgat tgatcagtaa ctgaagcata atattcaggg gacatataaa taaagctacc
      901 tagatagttt ttcacgattt cttttacttc gactttccat tctttttgat cttcatcata
      961 aagcgaaata tgatctcctg tagataagtc aaatagttct gctacttttt gcgtaatagc
     1021 gattcctgtt ttaggcaagg agcgtgggtt ctcttcaaga tcagttaaat ggataaaacg
     1081 atccaaatca gctgttttac ttggtacgat cactgtcaga ctttgcgaag catgtccttt
     1141 ttttctcaat tctacggttt gagtatgaat agctaggatc tcatttactt tttcttgctt
     1201 ctgcagcata tccgctgctt tttgacgact atcttccgtg gatgaatcca atgtaacgat
     1261 tgcttgatag tcaatgatcg gtccaaattg tttggctgaa acagaactta gagaatcttt
     1321 caatcccacg cctgcaacca tcagcccggc acatccagca attccgataa tcgccataag
     1381 cattcttgac ttatatcgga agagatttcg gtaactgatt ttttgattaa agctaaggcg
     1441 tgaccacagc ggagtgatgt attcaagaaa aatgcgtttc cctggcttcg gtgctttcgg
     1501 ttgaagcaat gcagcaggcc gttctcttaa atctttgaat aaaacgatca ttgcagcgcc
     1561 aaatgcggca atgaagaatg cggcagctgc ttgtaaaatc ggcggccagt cataaaaaat
     1621 aagcgcactc ccaatatcat accgctcgtt agacagctca aaaatgatcc ttggcaaaag
     1681 attcgttcct aatatcgttc ctaaaacaat acctatccct gaggctagac ttgcatagat
     1741 tgcatatttt tttgcgattt caactttcgt ataacctaac gcctttaatg tcccgatttc
     1801 cctgcggttt tcttctacca tacgtgtcat tgtcgtaaag gtgatcaatg cggcaataaa
     1861 gaaaaagaaa acaggaaaaa cattggcaat cgcagcgata cgatcagaca gatcaccaaa
     1921 ttcttgaaaa ccgacatttt ctgaacgttc attgtatagg tatgatggtt tagttaaatt
     1981 ttcaattttt tctgcatttc gattcaattc ttcctctgca gaggccagtt cacgttcttt
     2041 ctcaactaat tgcctttctt gttcagcaag ttgagcttct tgttgaggta atgtttgaac
     2101 agcgattgtt cgttgcgctt caggaagctg ctggatagtt gctgactgtt gcgctaactg
     2161 ttttttagca tcttctaacc gtgttcttgc ttgcgttgtt tgtgctttgc catccgctat
     2221 tttttgctgt gccggttcta attcttcgtt ggccgatttt ttcagctctt ctaagcgttc
     2281 ttcgggtcga tcttgtaaat acttttctgc cttttcttga ttttgctcca ttttatcttt
     2341 gtaagctttg ctgtacgtat tcaaattctt tacgtttgta aaatcaagat ataagacaga
     2401 ataaatatct gttttaaaaa cggattcagg taaatagaca aaataatcga cgctaccatt
     2461 tccgacgttc gcgtatcctc tctccaaatt gttgatgaac aatggagaac ggacaaagcc
     2521 cgtgatggta tactcttttt ctttcaattc atctgaatca atctgataaa tatcaccgat
     2581 tttataccct tcatcttccg ctttttcatc caatacaagc tggtttggtt tttccgggag
     2641 ttttccatca gttaattgta gcttgttctg cttgttattt ttgttataag aaaatatttg
     2701 aatcacttcg tttttgccag tattggaata atagatctgt tttcctgttt ctacttttgc
     2761 atctgggatc ttttctgctt gagcaatatc tttttgtgtc aagccaccag tagaaacgat
     2821 ttgcaaatca gaaagtcctg ctgtaccaac atagtgatcc atcgtttttt gcatgacagg
     2881 tccagcagtt ttgacaccta caaataaaag tgttcctagc atgacaataa ggatgattgc
     2941 aatgaatcgt cctttagatt gccgaatttc gcgaaaacct gctttaagat atgttcttgt
     3001 tttcataaaa tccccctacc atacaatctc atcaatggat gaaggatgat catttaattc
     3061 aacagacctt actttagcat cattaatgtg aatgactcta tcagcgatgg gcgctaaagc
     3121 agaattatgt gtaatgatca aaacggtatt gccgtgttgt ctgctagcgt tttgcaataa
     3181 tttcaatact tgcttgcccg tttcaaaatc cagcgcgcct gtcggttcat cacataaaag
     3241 aagtttggga tttttagcta gtgcacgagc aattgaaacc ctctgctgtt cgcctcctga
     3301 taattgggac ggaaagttat tcaatcgatg agctaggccc acttgcctca atacctctac
     3361 aggatcaagc gcatcaggag aaacttccgt tgccaattcc acattttctt ttgctgttag
     3421 attaggaacg agattgtaga attggaaaac gaaccctaca tctgttcgtc ggtaagcagt
     3481 aagctgttta tctgaaaatt gcgcaatatc tgtgtcatca ataatgatct gaccttcatc
     3541 aggtgtatcc atcccaccta aaatattaag aatcgttgac tttcctgctc cacttggacc
     3601 aagtataatc actaattcgc ctttttcaat tgaaaaagaa atatcgtcat ttgctgtaat
     3661 ggtcgtttct cccattttgt atcgtttgta ttcatttttt acttcgacat acttcactct
     3721 gattcctcca ttttcacgat atttctcgct atatctatgc atcgaatgcg ttttctcaca
     3781 cttatttatt ttaccactga atttctctag actaaagaaa ggaatccttt aatttagttg
     3841 acaaataaac gatttatgat ttacttacat aaaaaacatt gttatttgaa agaaggaaca
     3901 tcatgaatga tttaaaggaa aatatgattt ttgatttatc aaaaactgct tttgtcgcga
     3961 tcgacatgca aaaagagatc gtaaataacg gtacactctc tccacacact gcagcatcta
     4021 ttctgacaaa caatgatttg ttagtaagaa cattgaaacg taccgctgcc ttgaaagttt
     4081 tagtgaacgt agacatctcc actttccctt atttgagtca acaaactgat atgggtggaa
     4141 tgactgcttc tgtacctcca gaattcactg atttactttt gaaggattct ttgaaagata
     4201 cagataatat gttgactatc aaaaaataca atccaagtgc tttttttggt acttctcttg
     4261 atcttcaact tcgccggcgt ggaatcgaaa ctatcatttt aagcggtgta gcgacaacaa
     4321 acggtgtata tgctactgca ttagatgcct ttcaacacgg gtaccatatc gtgcttgctg
     4381 aagatgcttg cagtgatcgt gacaaagaat cccatcaatt gttcataaaa aagatatttc
     4441 cgaaaactgc acgagtacgc tcaacgaaac aaataatcga agctatccaa caaagcaaat
     4501 aagtactggc acacgtgatt ttcatttact tctttttgat ttcatgggaa aataaccaca
     4561 ggatttagat aggagtatga acatggtgaa aaaaaagatc acaatcaaag aagtcgctaa
     4621 actttccggc gtttcaatca caactgtctc cttgatatta aatggaaata cggagaaatt
     4681 cagtccgaaa actgttcaga aagttttagc tgccaaagaa gaattaggct atcagcctga
     4741 ctattttgct agacaaatga ttaccaagga aacgaaaaca attggtgtac tcgttcctga
     4801 tatcacgaat ccattcttca gtacactgat gcgaggaatt gaggatatct tgtataagca
     4861 gaatttcgtc acgatattat gtaatgctga ttctgatcat caaaaagaaa tcgaatatct
     4921 agccgagctc acacgtcgag gtgtggatgg attcattatc gcgacttctg ctgtctctac
     4981 agatgcaatc aacgaaaatt tgaaaaaaca aggccggccc ttcattgttt tggatcagaa
     5041 aaaatctgaa ggatttagtg atgccgtacg aacagacgat tttcgcggtg gttacctagc
     5101 gggcatgcac ttgcttagtc taggacatca aacgatcgcc cttgtctatc ctgaaaatcc
     5161 accggaaaat gtccacgctc ggattgaggg gtttaaatct gctcttgatg tttaccaaat
     5221 ccctcacgac caactgatcc tcttacctac acagttctca aaacaaggtg gctaccagat
     5281 cacagcagaa cttcttgatt ctgctgcaac tggtgtgttt gctttaaatg atgaattagc
     5341 ttttggatta tatcgcggat tagaggaagc tggaaaaagc atccctgaag actacagcat
     5401 tattggctat gataatatag atatgtgcga gtatatcaag ccgaaactga caacgatcgc
     5461 acaaccaatc ttcgaattgg gacaaacatc tgccaagtta ttgttagatc ggatccaatt
     5521 cccagaaaaa gaatgggaag aaaaaagatt acctgtacga ttagaaaaaa gattttcaac
     5581 tgcacccttg aaatagcttt caaggtgtgc tatattttta tcgaactatt aaaacgtttt
     5641 actaaaacgt tttaaaaaat aattttccca tcaaaataaa tgagatagtt ggaggtttgt
     5701 aaaatgaaca cggttacagt aattggaagt attaatatgg acactaccct tcgtttaacc
     5761 aacatgccca agccaggcga aacgatgcat gcgcatgaga tttttcacgc tggcggaggt
     5821 aaaggtgcca accaagcagt ggcagccaaa cgttcaggcg cacggacatc ttttattgga
     5881 ggcgttgggg ctgacagcga aggacaacag ttattagatc tactgacaaa agaaaacatc
     5941 gatacatctg ggatagctga aatccaaggc gccacaaccg gacaagcaat gattatggta
     6001 gacgcagcag gagaaaatag tatcttgatc catgcagggg caaataatgc tttccatgaa
     6061 caagaagtcc taaaaaacaa gcaactgatt acaaacagtg actttattat tgctcagttt
     6121 gaaagtagct tagatgcgac gatcctagct ttttctattg caaaagacgc aggaaaaaca
     6181 acgatcctga atcctgcacc agctcgtgaa acgattccta ctgaactact tgaaaagaca
     6241 gatatcatta taccaaatga aacagaaaca gaaatcatta caggaatcag agtgacagat
     6301 cataacagtt tggtcgcagc agccgaaaaa ctccatgaat taggtattgg aacagtaatc
     6361 attacactag gcagtgccgg cgctttctat catacagaaa aagaacacgg tatcgtccct
     6421 gcttttaaag tggatgccgt tgacacaaca gccgcaggtg atacatttat tggcgcatta
     6481 agcagcacac ttcagccaga ccttagtaac ttgaaagaag cgatccttta tggaaatcta
     6541 gcctcttctg ttgctgtgca gtcttatggt gcgcaacctt ctatccctta ccgcgaagct
     6601 ttagaacaat cagcagtcta aaattaacaa tgagaggaag aaacaaacat gaaaaaagga
     6661 aaagtaatca attcagatat ttcacgagta atcgcacaaa tggggcactt tgataaatta
     6721 agtatcgggg atgcaggcat gcctgtcccc tttggtactg aaaaaataga tttggctgtc
     6781 tcaaacggtc ttccaagttt tttatctgtt ttagaaaacg ttttagaaga acttgaggta
     6841 caatcgattg aattggcgga agaaataaag acaatgaacc cggaaatatt ggaaaaaatc
     6901 aaattattgt taccagatac accgattagc tttatcccac atgaagaaat gaaaaaagag
     6961 ttgaataact gtcatgcatt tattcgtaca ggcgaaatga caccttacgc caatattata
     7021 ttagtgagtg gagtggtctt ctaatggaaa cgacaatgaa tcctgaacag atggtactgt
     7081 ccgagcagat tcatatgctg attgaacagt tgggaggaaa agacaatatt gattctgtcc
     7141 ttccatgtgc gactcggctt cgtatcaaat tgaaaaaaca gccagtagcc aatgcagaaa
     7201 aattaaaaca gcaaatccct tccattaagt atttcatttt actcgataga tatgcagaaa
     7261 taatcatgga tcagggattg gctcctgctt acaaggaatt gacgcgtcaa atcgatcatt
     7321 cagctccgaa agaaaagcta aatccgatcg aaaaactatt tggtttgata tcggaaatct
     7381 ttacgccgat cattccgctg tttgccggtt ctggtatatt aaaaggtctg gttgttttag
     7441 cgactacgat tggctggctt agtgaaacaa gcggaactta tcatattttg tctgctgcgt
     7501 cgaatgccgt cttttatttt ctgccaatct ttcttgcttt ttctgctgcc aaaacgttta
     7561 aagtgaatgg atatattgcg gctgtcattg ctgcagcact gattgaaccg aatctgacct
     7621 ctttactttc agacagtggc aaagcagcta ctgattttct ggggattcct gtagtactga
     7681 tgcagtatac gtctactgtc atgccggcga ttttaggtat tttcttttat tcttttgttg
     7741 aaaaattcct caaaaaatat attcgcgaaa atatgcaatt agtatttgta cctttacttt
     7801 cattagttat cacggttcct ttgacgttaa tggtattcgg gcctgcgggt gtttaccttg
     7861 gcgaaggttt agctgccgca attacttgga tgatggatat taacggaccg atcagtggcg
     7921 caatcatcgc tggtggttgg aatattttgg taatatttgg tatccaatgg gctgtaaacc
     7981 ctgtcatgat cagtaatatc tctgcttatg ggttcgaccg aatcgttccg ttgacaggag
     8041 cagccaattt tggaatggct ggtgcagctt taggtgtatt tctgcgaagt aaacgttcaa
     8101 aaacccgttc gatctccggt tctgcttttg cttcgatttt acttgcggga gtgaccgaac
     8161 caactgttta cggaattgcg atcccattga aaaaaccatt tgttgctgct tgcattggtg
     8221 cagctgctgg tggagctgta atgggatttg cccaagtgaa agcgatcgct tttgtatttg
     8281 gtagtctgac cacattgcct gcattcattt ccggtacttt tttctggtac ttagcaggac
     8341 tggctgttag tctagtcgta gcaatgatca ctactctcgt tagcgggttt gatgaagacc
     8401 taatgtctta tgaatagtta atgaaaaaga ctgaagtatg agatgtttat cacatatttc
     8461 agtctttttc tttttaggat cattattttt ataaattttt gtgatacttt ccaaactttg
     8521 gtataattta catatgcata accattttta tccccacttg tttatcccca aacaaaggtg
     8581 gtctatgcaa taaaatgaac gtcaaagaaa aacgaatgaa tgctcgttgc ttatttacaa
     8641 attgttttat ttagaataca cagacagatt tatgaaaaga aaaagctttt cgacacgatt
     8701 atctgttcca aaaaatgtct aatgaatgct gaataagaca gaaaatcaat gaaaggaaaa
     8761 ggactagttt attatgaaaa ataaaaaaac gatacgtttg ctagctgcta cttggctgat
     8821 tgctccgtct gttttgtcga ccaataccgt atttgccgta gataatcaaa tagctgatca
     8881 aacagtcaca acagatacga ctacatccag ctcagctact agtgaaacaa cggattcttt
     8941 tacagaagac ccaactggaa caactaactc aacaacagga tcgaccgagc caactgaaat
     9001 tgatccggct gatccatcag aaaatgaaaa gccagccgag acatattccc ttacaattgc
     9061 tccttctttg attggtaaga tccaactgac tgcttcgaca gcggattccc ctgaacagca
     9121 acctttggat attacggaaa gtggaacggt agaaggttta ttagcggaaa cgaaggtaac
     9181 ttacactatt acgccagctg aaggtgaaca aattgcttca gtcgctgtta atggtgccaa
     9241 agaagtaaat tatgatgcaa agacctttac agtaggtacg aacaatttat ctattactgc
     9301 agaatttaaa gctggaccgg atacttcaac agagaaacca actgatccta gtgaagaagt
     9361 ccctagtaca ccagatccgg aaacgccaaa caaaccagat gtgggtaata acaacaataa
     9421 taatggttca aataattcaa acaataataa caataattcg aacagcggga attcttcgaa
     9481 tcaaacaaac ccatctggat cgaataacaa tggaaatact cagagagaac caaaccatcc
     9541 aaacaatggt tcatcgaatg gcggaacgaa caatgcttca tctcgtccaa atacagcaaa
     9601 tcaaagcggt atagagaatc catcttctgc ttcttctgat tttgttgtaa aatcaccaat
     9661 tgatgctgta ttgcctacaa atacaacgaa tgtccaacaa gcaatcgttc gagaagcatt
     9721 caagcatctt ggaaaaccat atgtatgggg agcaaaaggt ccaaatactt ttgactgttc
     9781 tggtttgact tattacgtat atatgaaagc aaccggtcat tatatcggtg gctggacagg
     9841 cgaacagcaa tatgcaggta cgcagattcc agtaagtcag gcccaacctg gcgatctcgt
     9901 cttctgggga ccttcttccg gtgttactca ccatgtaggt atctatattg ggaacggaca
     9961 atttatccat gcgcctcaac caggagataa agttcgagta acttctattt cagatttcac
    10021 gcctgatttt gctgtccgag taaacttagc aggtttacct gctgcttcag gttcgctgac
    10081 aggcggctca tctattttag atggattaga tggctcgttc catttcagtc aaaaccaaac
    10141 aactgatcag ttcttgaaaa agattgcaga tgatgcacag gaaatcgggc aaaaagaagg
    10201 aatctacgca tcagtcatga tggctcaagc tatccttgaa agtggatctg gaaacagctt
    10261 attatcaagc gaaccaaatc ataacctttt tggtatcaaa ggatcatata aaggaagcag
    10321 cgtgaccttc aatacacttg aacaagatag ttccggacaa agctaccaaa tccgcgcaca
    10381 attccgtaaa tatccttctt acaaggaaag tttggaagac tatgccgact tgatcaaaaa
    10441 tggactgact ggcaatcctg atttctacaa accaacttgg aagtcggaaa caaaagacta
    10501 caaagaagca accaaatatt tggaaggacg ttatgctaca gatcgccaat attcacaaaa
    10561 attgaacgcg atcatcgaag cgtacgacct gacaaaatat gatgaaccga aaaaagaagc
    10621 agcaacaact gaagaagcaa acgctgaaat cctcaatatg tctaagcgat tcgatgtgcc
    10681 aatcaaatgg acaacagatg gcgtgccaat cacaggatta gaattttcaa cgataaaacg
    10741 cgtcaacaac gcttcttcca tcttccgtgt cgcttcaatc tgggatttgt ggaacaattt
    10801 tacggcaaga cagataccag aaacaactac tcgtacagtt aaagctgcaa ctgttcctgt
    10861 actaaccttg ttaagtttag acaaatcatt ctggcttgat ctgcctttaa agaaatagaa
    10921 taaattaaaa aggggatgtg acactgtcac actccctttt taatttattc tatttcttta
    10981 tatttatttt gtattccgtt acaaactcgc gtacttggtt aggttccaga gtgacatctc
    11041 caaaactttc aagtcgctcc gctcctggca gttcttgagt ctcaaatgtg ataccgccat
    11101 gatttactaa tttattcccg cgcatttccg gtccatcttc tccaaaattt gctgtaaaaa
    11161 tcacgattcc tgaagcatct gtttttactt ggatcgatac tttttcatct gggcttgtca
    11221 attccgcagc atttagtaaa ccagattgtt ttttcaacaa aaatggatga tcgatgccgc
    11281 ccactatcat tgattggcga aaatcactgg caaagacctc acctaatttt ttaggacgtt
    11341 ggaagtcaaa tgctgtgcca cttactgaaa ctacttctcc tgtaggcgtt gtatctttat
    11401 tgagcgtagc aaataatcgg ctattcaacc aaagtatatg ttcgtcgatt gactgtgctg
    11461 gatcaccggt taaattgaaa tagacatgat ttgtaggatt gaatactgtt ttgcggtcac
    11521 tgatcccttg gaccgtcact cgccatgtat catcgttcgt cagcgtatac cgtacttcta
    11581 catctaaagt tccaggaaat ccgtttccca tatctgggct agtctgatag aaaatgaccg
    11641 atgcttcgtt ttcaccattg atgactgtat aatgccactg cttggcttca aagctaggtg
    11701 caccaccatg taaacaatga ccagtgtccg ggtctgtttt tacttcgaac gtttcatcat
    11761 ctaatttgaa tgtgccattc gtaatccgtc cagccatacg tccgattgaa gcaccgatat
    11821 aagggtcttt ttctagatat tcttttgctt catcaaaacc taagatcaac tctcgttttt
    11881 cattatcgat tattgtttga aaatgaacaa cccgtgcccc aagatcactg acagacaaag
    11941 atgtcccatt ttgattcgtg atcgtaatca agtgatagtt ttcgccaaaa ttttgttcat
    12001 tgatttccat gttccttgca ccctcttttc tttcatttgc actgattcca gtatacatgg
    12061 aaaccgtttt cttaacagct tattccgtga gtaacgagaa aaaatttact atattctaat
    12121 aaaagaccgt gaaaaaaagg ttgtgacaaa aatattgtca caacctcttt ttccgaacaa
    12181 acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atatgaggag
    12241 ctatttttgc ttggctgttc ttattacttg atgcaggaca gctttttcac aatctctttt
    12301 tcaatacgat tccagtatat aaccatgtcg tcagtacata gtagatgaaa tacaatacga
    12361 agaagatcgt aaacggaacc caaatatctt tgtatggatt ttgcatgaat agaccaaaca
    12421 tttgtaatcc gaataagaca tggactacac ctaagattcc tggtgctaag aataacacgc
    12481 ctacttctct acggatcgat tgtttaagca acccgcgtcg tgttcctatc ttttcaagca
    12541 tttcataacg agcaatgtca ctcttgcttc ctgacaagat cttgaacatc aaacagctag
    12601 caagcatggt caagaaagct aaacctagga agaatcccat aaattcaaaa ccagagaata
    12661 gaccattata cacttcatac atttcatatt tctggttgct gtactctact ttttgcaaag
    12721 atggattgtt cattttattt tgagcaacaa gtgtttcgat tggtttcagg tttgcttgga
    12781 agtcttttac ctgaactagc tgtaaagtgg attgtggcaa gttcagttga tcaaattctg
    12841 attgactgac taccttgatt tctttttctt gctgttccgg caattcatag cttcgaagtt
    12901 cctcttgtac agtcagttca ttcatttttt tagcaggaac tttttctttc gtgatcttcg
    12961 gcggtgttcc atcttcatgt tttatcatga tcaatggttg agccgcaaat tcacttgcat
    13021 tatagtaaat cgttcccgca tcttcttttt gagtataaga agcagaaagt gttggtgaaa
    13081 gttctttgat tttttgctga tcttctgccc gaggattatt catcactaga tcatatccag
    13141 aattgttttc tgtcaagtct gcaatttcat ttttaaaacc taatcctacc gtcaaagcgc
    13201 ccagtgccaa ggcaaacagc atagcgacca ttgagagcat ttgtgtatat tcacgaatac
    13261 gaaagtttaa ctgtgacaat gtgaaattgt tcaatttttt caatgaaatc tgttctgatt
    13321 tctttagtaa tgataaaaag aaaatgatca cagaatggaa aatgaaataa gttcctaata
    13381 tgatcgtaac taaggctacc cctataccaa agacttgata ggtccccacg tgtttcatca
    13441 tatagtaacc gcagcctaga aaagcaattc ccagtactac ttcaaggaaa aacaagaatt
    13501 tatgctgctt gattcttgtt ggtgtctgat ccgcacgaat caatgctaaa atcggttttt
    13561 tgacaatcga aaaagcattg acaacagcag ctaataaaaa caaaacagca aaaaacaaaa
    13621 cagtgatcaa caacccgtga atactaaaag gtgtaaaatg cgaaatattg agatctaact
    13681 gatccactaa taatcggtgg acgatacttg tcaatcctac accgacagct gaacccacta
    13741 aagtagctgc gatccctact aagaatgttt cagaaaaaat catctgtgcg atttttctgc
    13801 cttttgctcc cagcatcata aacattgcat agtctttttg ccgcatcgtc atcaaaaagg
    13861 aatttgcata taaaatataa acgaatgtga tgatccctag taaaacagaa ccgaatcgga
    13921 aaatgataac tgtagccgca acgatcgtat tacttttcaa aaaagcttcg ttgcttgcca
    13981 ttgattcaaa catgtaaaaa atagcagatg ccataactaa tcctgagaat aacaccaagt
    14041 agtctcttaa acggctcttg atcccagtaa gtgataattt ccaaagcata gtcaatctcc
    14101 ccctactgtt ccaagtttcc aagaatagat agtatttctc gatagaagga atcacggctt
    14161 tgatctccac gtttcacttc ttgatggata ccgccatcct tgatgaataa aatacgttgg
    14221 caataacttg cagaaaacgg atcatgcgtc acaagaagaa tcgatacatg atcttttgta
    14281 ttcaattcgt ccatcgtatc taaaagatcc cgggcacttt tggaatccag cgcacctgta
    14341 ggctcatccc ctaatagaat cgttggttgt gtgatcaatg cacgtgcggc ggcgacccgt
    14401 tgtttttgtc caccggaaat ctcagaagga tagctttcca aaatatgaga gatggataaa
    14461 cgttctgcaa ttttttggac acgttgcttg atttcttttg gtctgacgcc ttgtaaagaa
    14521 agaggaacag cgatattttc ttgtgctgtc aagctttcta ataaattgaa atcttggaaa
    14581 ataaagccaa tctcattcgc acgaaaatct gctagttgat ttccttttaa tgtggtcaca
    14641 tctttttgat tgattcggat atgcccatct gttggtttat ctaatgtgga taaaatattc
    14701 aaaagtgtcg acttaccaga accagaagcg cccatgatcc caataaattc gcctttttct
    14761 acatcgaagc tgatgccgct taatgcttgc gtctttttct cattgttttt tccgtatact
    14821 tttgttacat tctgtacttc gactattttg ttcatttctt tccctaccct ttaattagtt
    14881 tttccagtgc tgttttcggc acgtcttttt tttcgacttc ttcatacgaa gtgacacctt
    14941 tatttttgtt ccaggtgatt tttaaatatg ctcctttacg aagcggtcga gtcaattgtc
    15001 ccttgaagtc cagttctttt tccttaccct ctttcgtata gccaggcagg atatatctat
    15061 agttgactgt ttcttgtcca gagtctgatt ttgaaactgt ttttgtacca tctgtcgtaa
    15121 tttgtacata gtattcttct ccacccataa ctacgtaatc tgcgattttc acccctgcaa
    15181 gtaccacaac gatgaaaaca agtagaccga tcagaagttt tttcactttt tccccttctc
    15241 tcttacttta tatactgatt atagaaaaaa gcgaacaatt ctgccttcga atcaactatc
    15301 attcttcttt caattttgtc attttcttta aaacaactaa aaaaagagtt caagacaata
    15361 atccgtccta aactccttct tcctatctta caaaaagtat gtcacttacg gtctttcatg
    15421 tcgcctgtat tgataacagg ttgcgttcct ttgtctgtaa tgattgagac aagtaaatta
    15481 ttgatcaatt ggactttccg atcatcggtg aaattgattt cttgtccatc ttcgatttgc
    15541 tctaaggcca tttgagtgat tgtgacagct ccttcaacaa tggtttgtcg agcagacaaa
    15601 atggcttttg cttgttgacg ttggagcatg gcactagcga tttctgttgc ataggctaaa
    15661 tggttcaagc gtgtttcgat tacttcgaca cctgctactg ctaaacgctc ttgcagctct
    15721 ttttgcaact cgtcagacac tgccgtcgtg tttcctcgaa gcgtcaaatc attctcattg
    15781 aatgtatcat acgggtattg gctagcgata tgccgaatgg ctgtctcact ttggatttct
    15841 acaaaatctt gataatacgc tacatcgaat aatgcctttg ctgtatcaac gactttaaag
    15901 actactactg ctgagatttc gatggggttt ccatccaaat cattgacttt taaaacagaa
    15961 ctattgaaat tccgaacttt caacgaaaca gtcatttttt gtgccaaagg tatcgtaagg
    16021 aaaaagccat tttctcgaat cgtacctaaa tattgtccaa aaaacaaaat tacttttgct
    16081 tgattaggac tgactacagt tgccgaactt aacaacaata acgtgatcaa ccaaagaaaa
    16141 atacttaaaa aaagtgccca aactttcgct tgccacatac ctaaatagaa gaaaaacaat
    16201 ccaactaaag ctaatacgac caatccgaca atccctgcat aaccattgat ataaaatgct
    16261 cttttttctc tcatgctact tcctgcgctc ctttatcctt caataaatat ctaatgttac
    16321 ttttttaatt ttttttcgaa tacgcgccat tacaccttct agttctaact ctttgtcttc
    16381 agtgatgtac caaccccttg cctgtgtccc tccggctgcc tgcggtaaat aatccaattt
    16441 tgttcgaacc cagtgctttt cttctgtaaa atagtcttct accaagacct ctagatccat
    16501 ccctgctgaa agaggcaaat cttcccgaac agatttaaag ataaatcgtt ccttcttccc
    16561 atcatacttc aatattcctg tatcgataat cggtaaagaa taataatcca gctctttctt
    16621 caccgtcaat aaatgtttga aaacttcatg gtacacttgc tctaattggc gaccttctga
    16681 attgttcggg taattaacca tcactttttc tttgtctgga tcatagaagg tttggcttag
    16741 ttgagtgata taagtatgta atctttccat ttctgttttt aacttttctc tttgaccttc
    16801 ttctaccttc atatactctt tcaccctgtt ttctttttag atattttctt tcttatttta
    16861 ctatagttat gtgcgttaag cgatgaaaaa cagcaaagat tggaaggatc tttgctgttt
    16921 tttcttaaaa tatccagttg agtacaggat caatctgttt cattgtcctc ttgatccgtc
    16981 gtaatattga tcgatgtgtc tttagcagtg atctttagct ttttctgtcc ttgttctaac
    17041 aaagtatttg ttattggctg gtagtctttt tcaatataag attcaccaga cgtttggata
    17101 gtatagccac tgttttccat ctcatgcaaa gataaattgc caccgtttag gatgactttt
    17161 ccttctttgc tgatcaaact gtcttctaga tggaagttgc tgttttcacc tgtgacgatc
    17221 aagttatcca cattggcctc atctaatcga agtgagccta attcatcttt cgcagttatt
    17281 gttcctcctg taaattcatt gaacgatacc cagccatcat gtgtcttagt attcactttt
    17341 ttgattgata cttcgttgat atcaattgca ctattattcc cttcgattgt caattcagat
    17401 aattcatgat ctgcagggac aacaatagtg atttgtgcac ttgtgtcttc taaactaatg
    17461 attgcacctg ttgatttttt gactgcattc acagtcaaca cgccattttt ctcctttatt
    17521 tctggctttt ctgtcttatc ataagtcgtt tttatctcaa aatccaatcc gcgtatgacc
    17581 gaaactgttt gattttctgc atggatgacg attttgtttg catccttgat tttttttgtc
    17641 tcagtaacca tatcgatcaa ccgaggacca ttatcccaag tcagactctt ttgagcgcct
    17701 aatgtataag ttgcacctgc taggaggcca ccagtaagca tcagaccaat accggtaatc
    17761 aaaaaatgtt tcaatctcaa tttatgtgtc ctccttcaaa cgcccgctga atgcagatct
    17821 ctttttgtaa taaaacgtcg tcccatccat ttaacaaagt tcatcatgac ataaaaaagc
    17881 ccttttgttc ctttcagcaa cagtggcgaa ataaatactg cgcttcctgt ggcgaacaga
    17941 ccgacaccga taaagaataa tccactcaga atcgattgtc caaacacact gattcctgag
    18001 atgatcatga ataggccaac acctaagata ctgatacata aaacatagat actaaaaatg
    18061 agcgctagta aagcaaccaa tagtccccca ataaccaata taaaccccag tgctagtgga
    18121 atgaggacag gagaagcaaa taaaccgata atgattaacc aaatcaggcg aatttgtctt
    18181 tttggatttt tcactaactc gttttctgct tgttcttctt ctgaaaagta actgattttt
    18241 aattttaagg caaactgctt tggtgtacca tattcatcaa tgatctgatc taatgttttt
    18301 ccagaatcta aaaaatattc ctcataatac tggatcaaat caaaacgatc gtcttcagga
    18361 atttcttgaa gatgatccac aacttcattc aaatattcat tcatttgtgc tctcctccaa
    18421 taaccggtct aaagaatctc gaaaaacaat ccattcatca ataatttctt ctaaatgacg
    18481 acttcccatt ggtgtcaacg agtaatatcg acggtttctg ccttcaaaag gttcatcata
    18541 ggtctcaaga tatccttctt ttttcaatct tctcaaaacc ggatatagtg tagattcact
    18601 gacagagaga ctttcttgta cgatttttgt taaggcatac ccatacatat cttccttgct
    18661 taatgctgct agcacagtac catcaagaag aacagttgga atctggatgt gcattacgtc
    18721 accttctatt ctcaagtgta atattttatt tcaatctata ttatacaaca tataatatag
    18781 tttcaaaatc agtttgtcaa atacaaaaaa actgactaaa agatttgatc ctttttagtc
    18841 agcaaaaaat ggcttctctt tgatttttat tataacagtg gcataatcca atttgtccac
    18901 atccagctca gtggcatgat aacgatcatt gcaggtatca tgtcagctac tgggaacatt
    18961 ttcacgttga tcattctaaa accggtagcg accatcaaga atccgccgca agctttaaaa
    19021 tctaagatca tgtctggtgt agtcaatgga taaataaatt ttgccaataa gaacaaaata
    19081 taaaaaataa tgaattgggg aattgctatc acagaaacga cataccctaa attacacgca
    19141 aaaatagctg ctgtgaaaaa atcaaggaca gatttagaaa tcaaaatcgt actgtctcct
    19201 gtcatcccag aatctaaact tccatagatc cccgtaccac ttgcacaaaa tagaacaatg
    19261 accgtcacta atgtgctgat aaattcatcg tgggtcatac caagcttttc actagggaag
    19321 attttagaaa taggtacttg catcagcgca gcccccttat ttatcccatt acctaaatga
    19381 acaatcagac caatcgctgt tcctatcact aaagcaaaaa taacagccgg catatttttc
    19441 atcggcgcaa tggaatagat tcccatcccc atcgaacaca caccaaaaac catcgtcagc
    19501 tgtgctttaa aatgttcact taatttattt cctactaatc cgccaattag tccgccaagc
    19561 aaaacggaac ctacattgat caaaactcca gttggcatca aaaaactcct ctctttttct
    19621 acgaagcgaa aaaaatcaga aaaatttctg taattattgc tttcgctgca agaaagattg
    19681 taacagacag agaagattac accaattcat tttttgtgtt aaagtattct ttattggaat
    19741 aagtaggagg aatatgatgg atatccataa attcgaagtt tttcttgatt tagccgaaac
    19801 aatgaatttc accaaaactg ctgatcgaca attcaccacc caaggaaata tttctaaaca
    19861 gattctttcg ttagaaacag aattagacgt caaattattc gaacgcgccc atagaaaaat
    19921 cgaattgacc gaagctggaa gcttattgct cccatatgta aaaaatgtcg tagaacaata
    19981 ccatgctatg caagaagtgc ttgaaaccta tacgaaagac aacaatcttt cattaaatat
    20041 cttaacgata ccgacaatga tcaattataa aggtttttcc aaaatcaccg agtttttgaa
    20101 acgccatcct gaatttacag tccagttgaa agaagtggaa agtgtcgaat taacttacaa
    20161 tgacgccaat gaacatagtg acacgatcca ctttgctaga aatttccagg ccccaccaag
    20221 ttcgattgag tggttgccta ctgaaacaga cgattttgta gcagttctac ctaaaaatca
    20281 tcctttagct agtaaaaaga aattggatct aagcgaactg aaaaaggaga actttttgct
    20341 ccttggcccc actacgaact tgtatcaacc tgtactagat ctgtgtcgcg aagctggatt
    20401 tgaaccaaaa atctcttata aaggagcaag aatcgacttg atcatcaata tgattgcaaa
    20461 tgacatgggg attgctattg ttatggaaaa aacagtcaag aatctattaa aagaaaatac
    20521 tgtgattctc cctatttctc caacaacaga gagttatctt gcatttactc gtaaagtcgg
    20581 tgagcattct ttggcttcag atacattttg ggagtattta aatcaagaag aaaactaagc
    20641 cctacatttt ctgtccatct atccacccaa aaaaagagag cgacgcatga ctttcgctct
    20701 ctttcttgca tcatgtcctt gtttcaatga cacctctaac atttctcgta gttcaataag
    20761 aaagcagcga tttttatgcc aaaagaaccg taagaacatt tccctaatca caaaaaaacg
    20821 actaaaaacc cctaaaaacc ttcaatagaa tttttagggg ttttattctt tttcctattg
    20881 tttacttaac cgtatcattg ctgtcgttcc tactccttcg atactgctat aatcaatacg
    20941 tccatcatgc agttctagaa tttttttaac cagtgaaagt cctagcccat tcccagaaat
    21001 ttgacgactt gtatctcctt gataaaattt ttcaaacatt cgatctctgg tctcttcatt
    21061 cattcctaca cctgaatcgg tcacttccag tccaatctct gtggctgtct caaatagttt
    21121 gatatgaatc tgtccattga tttgattgta ctttatggcg ttatccatga tattcagcca
    21181 cacttggtac aaaaactctt cattgcctgt gtaatttact gcagcaagtt cgatatccaa
    21241 ttctagctgt tctttctccc atttaggctg caaaaaaacg attacttctc tgatttgttc
    21301 atctaatcga tactctttct tttccagttg gatacgctgg ttttccagtt tgttcaattt
    21361 taagatgttc tctgttaatt gtgaaagctg cgtgatactt tcgataatcc gatataaaaa
    21421 tatttggcgt tcttcgtcag agagatttgg ggcttgtaac aattgaacat atccttggat
    21481 tgttgctagt ggtgttttga attcatggga tacactggac acaaagtcat tcctcagtgt
    21541 ctcgatacta ttgagttctt ggaccataac gttgaaatct ttatacagtt gctgtacttc
    21601 tgctactttc tgctgttcat ccattcgaat cgaaaaatct cccgttgcta ccagactcat
    21661 atttgtccct agcgcactga ttggatgcag gatacgccgc ccaacaaaaa tcgatattcc
    21721 tgtacccacg accatactga accccgcaaa gacgaataac gggtataacg ttggttttcc
    21781 agccgattca gcaggcgaga tagaatgtcg agacaataag aaaataaaac cgagaaaaca
    21841 cagcagtgtc actaagatcg aaatgaagac aatactagca aaatagatcc ataattgtga
    21901 aaatagcttt tttttcatct ttaaataacc gccttatagc ctagtccccg tactgtgatg
    21961 atttgaaaat cttccgtctt ttcaaaacgg gtccttaaac gtttgatatg tacatcgatc
    22021 gttcgttctt ctgtattcgt atctaatccc caaatatcat ccatcaactg ttggcgagtg
    22081 aagatcttgt tgggataaga cagtaattta tacagaagca gaaattcttt ttgaggcaga
    22141 atcaacgaat gctgatcttg taacactgta taagcatcta gctctaattt tgtttgtccg
    22201 attatcaagc tactgctgta attgatcttt gaacgtcgaa gcaatgcttc cacacgtagg
    22261 atcatctcat tgacatcgat gggttttgtc atataatcat ctgctcctaa tttgaacccg
    22321 cgtttcttat cctcaaaatc ggcctttgcc gtaatcatta gaacaggtgt gtcaaatctg
    22381 gtgctacgta atgagtctag caagtcatag ccatccattt tcggcatcat gatatctgta
    22441 atgattaatt ctatacgtgt cgcttctagt tttttcagcg catcctctcc attcaatgcg
    22501 gtgatgactg aaaagcccgc tcgttccaac acagaacggt aaagcaactg taattgcgga
    22561 tcatcttcta ctactaaaat acgattcatt gatctccctc tgttttttga aaaatttcta
    22621 gattttttct tgtagttcat cttcagttaa catttatttc gtatactacg atcattgttc
    22681 tgatatactt attagtatac cgaatatttc aatacgggag aagggtgatt atgcttcaat
    22741 taaaagatat aaaaaaatat tataaagtcg gcgaaaccac caccaaagca ttggatggtg
    22801 tgtctgtcgc tttcagacaa aaagaattcg tagcgatttt aggtcctagt ggttctggta
    22861 aaacgactat gctgaatgtg attggtggac tagataatta tgattccggt gatatggtaa
    22921 tcaacggaaa gtctactaaa gactttaagg acagtgactg ggatgcctac cgaaacaact
    22981 ctatcggttt catttttcaa agttacaact tgattggtca tctaggcatt attgaaaacg
    23041 ttgaacttgg gatgacatta agtggtgttt ctaaagacga aaaacgaaaa cgggcagaag
    23101 atgcattgca ccgagtcggc ttgacagatc atatgcataa aaagccaaat cagttatctg
    23161 gtggacaaat gcagcgagtg gccatcgctc gagcactagc caatgaccca gatatccttt
    23221 tgtgcgatga accaaccgga gcgttagata cagaaacaag tatccagatc atggaattga
    23281 tccaagagtt atcgaaagaa aaattagtga tcatggtcac acataatcct gaactggccc
    23341 atcaatacgc agatcggatc attgaatttt ctgatggtaa gatcttaaca gattcccatc
    23401 ctcacatcga acgaccaaaa gatgatcaat ttaacttacg cagaacaaag atgagttttt
    23461 ggacagcgtt aaagctttcc ttcaacaata tccgaacaaa aaaaggacgt actttcttga
    23521 cctcctttgc ttccagtatt gggatcatcg gaatcgcgat cgttctgtcc ctttcttctg
    23581 gattccaaaa acaaatcgat aacacgcaag ctgaaacgat ggcgaaattt ccgattacta
    23641 tttctaaggt aacgactaac cagactcgtg acgatgccgg attgggtgca agcaaagcgg
    23701 attatccaga cagcaaaacg attactgcaa aagtcagcga agaagataaa gcacagcaca
    23761 cgaataagat tgatcaaaca tacgtcgatt acgtaacaga catcgatcct aacttaagta
    23821 ataatatcgg atttacccgt actacaggaa tcaatctttt acgtgatgta aatggcaaag
    23881 tacagcctgt cagcttctcg aatcaaaatc ctgatacgga atctctttct ttatctagta
    23941 cgatgtcagc aatgacaggt gtgggagtgt caagtttccc tacacagctt gacactagca
    24001 aagaaaactt tttaaaagac aattattctt tacttgccgg aagttatcca gcctcagcaa
    24061 cagatgtcgt gctgatcgta gacggcaata ataatactaa tatcaacgca ttgaagaatc
    24121 ttggatttga tgtgaaagaa gacgaaaaac ttgattttga tgatatcgtt ggtacaacat
    24181 tcaaattagt aaacaacaat acttactata caaaacttcc aactgggaat tttatcccta
    24241 atacagatta tgatgcgatg tatcagaatg cttctgatga attgaagatc tcggggatcc
    24301 tacgcgtgaa atcttcttct acaatgaatc tcttatctcc aggaatcgct tatagcgatc
    24361 aattaacgac tcaaatcgtc aacgaaaaca aagaatcaga aatcgtcaaa gctcaaagag
    24421 acagtggtgt aaatgtctta actactgaaa aagttgacga aaatgccaaa caaacattgc
    24481 ttagttattt aggtggagac agcttgcctt caagtatcat gatttatccg aacaattttg
    24541 aagacaaaga aaagatcttg gattatctag atgattacaa taaaggcaaa tcagatgaag
    24601 ataaaatcat ttatactgat ttagctggaa cgatgactga actgactggc ggattgatgg
    24661 atgctatcac ttatgtcctt attgcttttg cagggatctc tttagtaaca agtatgatca
    24721 tgatcagtat cattacttat acatctgtga ttgaacggac aaaagaaatc ggtgtactaa
    24781 aagctttagg tgcacgtaaa aaagacatca ctcgcgtatt tgacgcagaa acgtgtattt
    24841 taggtatttc ttctgggata ttaggtgtgt ttattgcatg gctagctacg ttccctatca
    24901 atagcatctt atacaacatg acggatctaa aaaatgttgc tcaactaaat cctgtccatg
    24961 cgattattct agtgatcgtc tctactatat tgacgatgct tggcggtcat cttcctgcac
    25021 gaatggcagc gaaaaaagac gccgctatcg ctttgcgtgc agaataaggt tgtgacaacc
    25081 acatttagat ttgagtcata agagcgctga aatagtagaa aacagcaaga aacgaggaag
    25141 taatatgttc cttgtttcgc gctgtttttt ttaattattt tttataattc tggtatactt
    25201 ttctttcctt tgtaccacaa ctcttctggc gtatacctgt cttttccatt tatgaatact
    25261 gttccatctg cttcaatcgt tacttcagtc ccaatccaca tattacgaga aagtgcgaga
    25321 agattttttc tttttcctct tccttttttt ccaaattgca aggcatcggt tatctcttct
    25381 gtccaatgag atttcacgta ttttacttct ccgttttcaa atgtaacttt caaaatattc
    25441 gctatatttt ccacttttac tgctttagga aatgactcct tcattttttt cactttcctt
    25501 cctccaacac taagtatgcg ctttctttta gtgataagta tatcatagtt tattctatta
    25561 tcaaaaaaac gcatccattc cattgtagaa tactttaccg aacaagttga attgttttcc
    25621 tctttttcac cttttactat agataggtaa agacaactaa ggaggaatct ctcatgacag
    25681 atcaaccgta tgatttaaga aaagtcatcg aagaactaaa aacacttcca ggtcaatatc
    25741 acgaaacaga tattgaaatc gatcctgaag cagaattatc tggtgtttac cgttatatcg
    25801 gtgctggcgg tacagtaaaa cgtccgactc aagaaggacc agctatgact ttcaacaata
    25861 tcaaaggttt ccccggcaca cgtgtgaaca tcggaacaat ggctagtcgt aaacgtgtag
    25921 gtgaaatttt gcaccatgat tatcgaacac ttggtcgttt cttgaaagac gctgtagaaa
    25981 atccaatcaa acctgtgaaa gtagacaaag cagacgctcc agcacaagaa gtcgttcatc
    26041 tagctaccga tgcagacttc gatattcgca acattttgcc tgcacctaca aatactcctg
    26101 aagatgctgg tccatacatc acaatgggtg tagtcttagg atcagatcca gaaaaaacaa
    26161 tgagcgatgt aacgatccat cgtatggttc tagaagataa agatacgatc ggtatgtaca
    26221 tcatgcctgg cggacgccac attggtcact tccaaaaaca attcgaagcc ttgaataaac
    26281 caatgcctat cactatcaat attggtttag atccagcaat tgccattggc acgactttcg
    26341 aaccacctac cactccactt ggctacaatg aactatggtg tgcaggagct ttgcgtaacg
    26401 aacctattca aatcgttgat ggtatagcag tcgatgaagt gggtattgct cgtgctgaat
    26461 tcatcattga agcagaaatc atgccaaatg aaacaatgca agaagatatc aacacgaact
    26521 caggaaaagc aatgccagaa tttcctggct atactgggga tgctaatcca gctgtacaag
    26581 tagtgaaagt caaagcagtt actcaccgaa aagacaatcc aatcatgcaa acaacgattg
    26641 gtcccagtga agaacacgta tctatggccg gtattcctac agaagcaagt attcttgcct
    26701 tggtcgataa agctattcca ggaaaagtag taaatgtcta caatcctcct gcaggtggtg
    26761 gtaaattgat gaccatcatg caaatccgta aagaaaacga agcggatgaa ggtattcagc
    26821 gtcaagcagc tattcttgct ttatcagcct tcaaagaatt gaagacagtc attttagtcg
    26881 atgaagatgt tgatattttc gacatgaacg atgtagtttg gactttcaac actcgtttcc
    26941 aaggagataa agatatcatc gtgatcccag gaatgaggaa ccatccattg gacccatcag
    27001 aattaccaga atataatcct ggctctattc gtttccgtgg gatgagtgca aaaactatct
    27061 ttgacggtac agttcctttc gatatgaaag aacattttgt acgtgcaaaa ttccaagaag
    27121 tagaagattg ggaaaaatat ttgaaataat gaatgatctg caactgtttt gacatcataa
    27181 aaaggagtgc ggaaaatgga acaattggca caacggaaga aaaaaatcgt gattggtgta
    27241 agtggcgctt caggcaccat ttatgccatc aacctagtga agaaactaaa agaatatccg
    27301 atgatagaaa cacacggaat catcagtcca tgggcaaaac agaacttaaa gctggaatcc
    27361 gacttatcac taactgaatt cgaaagcctg tttgattacc attattcaaa caaagattta
    27421 ggtgctgctg tcgccagcgg atcatttttg accgatggaa tgatcatcgt accggcaagt
    27481 atgaaaacag tcgcaggtat ctctgttggt ttaggcgaca acctgattgc acgtgctgct
    27541 gatgtaacaa tgaaggagca acgaaagtta gtcattgttc ctcgtgaaac gccattaaat
    27601 gccattcact tggaaaactt gactaaatta gcacgactag gtgtgcaaat cataccacca
    27661 attcctgctt tttataatca gcctcaaacg atccaagatc ttgttaatca ccatacaatg
    27721 aaacttttgg atcaatttgg aattgtaaat gagcattcaa aacgttggca gggtgattaa
    27781 tatggaaaaa gaaacgatag ttgaagtaac caaactaggt ttcacgatcc aagctgctgt
    27841 taaagtaatc ggtaaagact tattgatcac tttaacaggc ggagatacac cacatatcgg
    27901 aacagtcact acattttctc gtgatacaga gatgcaaact gtacgtttcc ctagccacga
    27961 tggtcggttc cataaagacg atgtcttagc cgaaaaaata gctgtgatca tcaaaactgc
    28021 cgttccggga agttgtacga tcacttccgg tatccatgtt gaccatatct ccaatgcaca
    28081 aatcaacgcc tcttttccga tgtcagaaga gttaggttac aagattttag attggttgga
    28141 aaaaatagat ttccatatca aagaaccagt ttattataaa gatggtgaaa aaacattatg
    28201 aaagaagagg ttgtgaaatc agcgttatga cctgagtatg agagaaaaaa atggaaaaat
    28261 agcttctcat attttgccgt ttttgagcta ataccgaggg acagctgatt gaacgccgtt
    28321 tatcaagagg ttgtgataaa gccgcttcgt ttcgagtagt aagaaaaatt ttgtaaacat
    28381 gggtttccac atttttgcat aaatttgact tactaccgag aaactggctt ttgaacaccg
    28441 tttatcaaga ggttgtgaca cgtcttttgt cacaacctct tttatttttg cttcactgta
    28501 actgctacaa tatcaccgat ttcttttccg atgtttttac gaatatcttt tcgtagtcca
    28561 aggatgtagc agactgaacc atctggattt ttcacgccca tattcacgat actgccttca
    28621 tacggcatat tgtcaaaagt tgcttggact ttaactcttc cagcaccgaa ttcttttctt
    28681 atatcatacg gaaaaataat atatgccccg cccttgtcga cttcggaagc atagattttt
    28741 gattcaaaag aataaatttc cctcatttta tttctcctat tttttacatc tagtatagca
    28801 tagcaaaaaa aagaaggaac tgtgacaaaa gtcttgtcgc agttcctttt ttcgaataaa
    28861 cggtgttctc ctgccaactc ctcggtcaca agtcacaata gtcaacaaac atgagaagct
    28921 gttttttgaa attgctcctt gtgactcaga gttaaacggc aggctcacaa cctcttcctt
    28981 aaacggtgtt caaaagcttg catctacggc actaagccca atcagatgaa aaatatggag
    29041 tgctattttt atctgattgt tcttactgct tgatgcagag cagctttttc acaacctcct
    29101 acatggtgtt caaaatatta ttcgcctttt gcttctttca ctaattttgc gaatgcttgt
    29161 tgttggaatt cagcagctaa ggctttcgta cgttgttctt gctctgcttt gctcattttt
    29221 ggattttcag tcattgcttt taccataccg tcttgaacat aagcttttgc ttcactttcc
    29281 atcgtacttt gctgtttctt cactaattca gctaacttgg ctgcttggtc cgtacttaat
    29341 tctacccatg tctctgggat tttgaatgta ttcactcggc tgtcatattc gtgattgtta
    29401 tcagcaatat tgaaccagaa tttatacatg tcgtttttgt attcccaata agtagttttc
    29461 gtcacttttt cagcatcgcc gtctgtcttt tctactttat ttgtttcgtt atccgtacca
    29521 gttgcttttg gtttttcttg tttttcattt gttcggtaaa gatacacttt ttcttttcca
    29581 ttccctaaag ctttatacaa aagcatatct gctccttggg aatcagcact ggaaacaatt
    29641 ttttctgttt ttgtttccgt tactttttcc attccaaagt gattggacaa attagcgacg
    29701 atcaaactta gtgaagcgac gaagattagt ccgaaaatca aagataaaaa ggtttgccat
    29761 gtttttttag cgaacacgtt cgttaaagca aaagcaagca agctgacaat caaaactcca
    29821 ataatcatgc attatccccc gctttcatta gaacaggttc ttttttcttc aataaaaagg
    29881 caataataaa gccaagaatc ccaaagatga tcgctacgat aaacgcggca tggtaacctg
    29941 acagtgtggc attgattgcc tgatctttat aagctaatgg cgtattatcc aatacagatt
    30001 tgcttgggat atttgctttt gtcacattcg tcaacacact gatcaaaata gctgtaccaa
    30061 tcgagcttgc gatttgacgg aatgtattat ttaccgctgt cccatgactg atcaagttat
    30121 ttggcaatgc attcatacca gaagtcgtta ccggcatcat gaccatggag atcccaaaca
    30181 tacggatcgc ataaaggatc acgatatata aaactggagt cgtttttgtc aagaaagcaa
    30241 atggtgcagt tgccgctgtc aaaatgaaca tccctgtgat ggctaaacgt ttcgcgccgt
    30301 atttatcgaa aatcgcaccc gtcacaggca tcatagcccc catgatcaat gcacccggca
    30361 acaacatcaa gccagaatgg aatgctgatt ctccacgaat attttgaata tacaatggta
    30421 cgaccatttc tgcccctacc atcgccatat ttgttacacc agctaaaaca gctgcaatcg
    30481 tgaatgtaga tgatttgaat acacgaagtt ccaagaatgg atgttccaaa tgtaattgac
    30541 gccatgagaa caaggcgatg aaaatcacac caacaactaa gaaaccaatt actttcaagc
    30601 ttccccagcc gtcatctcct acactagaaa atccgtaaag taaactaccg aatccaatcg
    30661 ttgataaaat agcggataaa atatcaatgc taggatttga caatggaata acacttttca
    30721 ttaggaagaa tgctaaaacc aagacaagaa caacgattgg cagtaccata ccaaacaagt
    30781 cacgccaatg gtagttatca atgatataac cagataatgt agggcctaaa gcaggagcaa
    30841 gtccaataac gatccctgac atccccattg ctgatcctct tttttctggc gggaaaatag
    30901 atagcatgat attttgcaat aatggcatcg atactcccac gccgcaagct tggatcaagc
    30961 gaccgattag taatgtacta aaattaggtg ctgtaaagca agtaatcgtt ccgatcaaaa
    31021 agatcgtcat ggcagaaaga tataaatttc ttgaactgaa tttattgatc agccatgctg
    31081 ttattgggat catgatcccg ttgactagca agaaaccagt cgttaaccat tgcacatcag
    31141 acgctgaaat atcgaaagct ttcatcaatg taggaaaagc tgtcgtcaat aaggtctgat
    31201 tcaaaacggt acaaaatgta ccaattaata agacaacaac taaaagtgtc cgattatatg
    31261 gttttccgta aatatctacg ggttggttgt tactcatatt tctccttctt tctgaatttt
    31321 ttctgaaact actagaatac tttagtccta aattcgctat ttgtcaacct acttgacaca
    31381 taagttaata cggatataat taagggaagc aagaggacaa aaggagtgac aaaaatggtt
    31441 ggggcttccc ttaaacgttt gttggaaaat gatcaattgg ttgttggcat cagtgaatta
    31501 agtgatatcg taggtgtgtc tccacgtcaa ttaaggtatt gggaacaaaa gggattcatt
    31561 cgttcgatcg aagctgatgc gaattgtcct agaaaatatc gtttacctac agtcataaaa
    31621 gtagaactga tcaaaaaata tttagatgac ggatttactt tgtcaaaagc tgcagaaaag
    31681 gcagaaatcc gccaaaagaa aatgcaccat gtccgtaaag ttttttcaaa agcaatcaaa
    31741 gatatcgaat tgatcgacga acgctatacg attttacaaa tcggtaactt caaagaagag
    31801 aatgctaaga tgtacatcat tcatgacaac gagacagacg aattaactta caagctcctt
    31861 cctgtcgatg aaaaagctga ctacgaacag ttgttaaaag aactctaaac gccactaatc
    31921 tcctaagtac caagcttttt aataagtaga tgctacataa atacttgaaa aaaggcctgt
    31981 ttgaacataa gaatatccga acggtgatct gcgtttcttt catatagaaa ggaattctcc
    32041 atcactgttc ggatattttt ttattttttt cggttacttt cttctaatac atctgttagc
    32101 caatttgcat attctgtttc ccgattgatc gcatgcgcta aaatcaggta gtgtccgtaa
    32161 ttttcatgtt tttctttttc agtaggaaag atcgtttttt ttcgctctaa tagatgaagt
    32221 cgtttttctt catgaaggtc acgctgctgc tgaataattt ctactaatgc cggatcattc
    32281 acgtctttga cgaaatacaa tttcagaaca aattcgtctc gattaaccgg tagttcattg
    32341 gttggtgtat gaatccactc tgccaattgt tccttccctt cttctgttaa ttcataaact
    32401 ttcttttcca atttttcacc tgtgatctct acttgatgct gaatgattcc ttgctcttcc
    32461 atttttgcaa gttcaggata aatctgacta tgttttgctt gccaaaattc tcctatttca
    32521 tttttgaacg attgattcaa ttcatatcct gtcatcttct tcttacttaa taaccctaac
    32581 aaaatatatc gaagtgtatt ttttcttgcc atcagttttc cctccttgac aaatctttaa
    32641 atccattcta tcataaatga gtaatcgaaa acatgtagtt tattacatgt tttaatttat
    32701 aaggagatgc ttaaacatga aagaatttaa agaattggaa gattttattg gaactcactt
    32761 tatctatacg tatgacaatg gttgggaata tgaatggtat gccaaaaatg accatactgt
    32821 tgattaccgt atccatggcg gaatggtcaa aggccgttgg gtaaaagacc aagaagctga
    32881 tatcgttaaa ttgacggacg gcgtattcaa aatcacttgg acagagccaa cagggacaga
    32941 cgtggcattg gactttatgc caaatgaaaa gaaacttcac ggaatgatct ttttccctaa
    33001 atgggttgaa gaacatccag aaatcactgt tactttccaa aacgaacata tcgcagaaat
    33061 ggaagcagct cgtgaaaaat atgaaactta tccaaaatta cttgttcctg aatttgctac
    33121 gatcacttat atgggtgatg caggtataga caatgaagat gttattagcg aagaaccata
    33181 cgatggaatg ccagatgata tccgtagcgg aaaatacttc gatgaaaatt ataaacgagt
    33241 aaaataaggt tgtgaaagag aggttgtgaa atcagcgttc tgacctgagt attagagaaa
    33301 gaaacggaaa aatagttcct catgttttgc cgttttttga gctaataccg agggtcagct
    33361 gattgaacac cgtttatcaa gaggttgtga aatcagcgtt atgctttgag taataagaac
    33421 gctagttaga aaatagcttt tcatattttc taggctagtg ggcttattac cgagaagcag
    33481 ccgtttgaac accgtttatc aagaggttgt gaaatcagcg ttttgacctg agtattagag
    33541 aaacaaatgg aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca
    33601 gctgattgaa caccgtttat caaagattgt aacaactgca tattcacaca gttaaattag
    33661 tgacttcaaa atgaaataat gctcccagca aaaaattagg ctgggagcat tatttcgtta
    33721 ttctgttcat caaaaatcag cgttttgatt ccatttcatc acaagttcct cctcatttac
    33781 tgcttcatct ttttgctgtg tttgaattgt aaaaccttgt gaaagataga aatgaatagc
    33841 tgattgattt ttttgataga ctgtcaaagt cagtgtttta ttggtttctt ttattttttt
    33901 cattagttgc gtaccgattc tttgttgccg gtattccctt ttcacaaaga ttcctgcgat
    33961 aaactgatca atcaccccag caaaaccaat aatttcttga tctatttctg ctaagtaaat
    34021 cactgcatct ggtaaagctt tcctaacaga ttctacttga ttctcccaat aagttgatgg
    34081 aatgaattca tgagaatcca agttagcatg cagccaaatc gatagaacag attccagttc
    34141 tttggcagac aattttgttg ttgtgcggat cattttttct ctccttactt attcattgat
    34201 tttaaaataa aaaccatttt tttgaagctc ttttacaagt tttcgcccct caaaaaagta
    34261 tatcctctct tgttttactt ttctaatacc aaaagaaaca gcaactttag gatcatcagc
    34321 tgaaacgatt cgataagttc caccaggcaa ttgatccagc ttgactttcc catgatggtc
    34381 gctttgtact gaaaacggca caccatttct agtaagtgtt tttttcttat gtttcaaaat
    34441 gaatgaacta ccatttaccg gtttccctga cgaatcacta ataataaatg aaaaatcaag
    34501 cactttttta cttaatctat actgatagat tagtcgtccg ataaacacta acagtatgac
    34561 tacagctatt atcgttcctg caagaataaa ccattgccgc atggttcttt cctgatttcc
    34621 ttttccttct tcttttttta ccgattcgtt ataaggaatc cgatgtcctg tcactagcaa
    34681 acgatgcgtg ttgatcatat acggcgtaca tgtaagcaag gtaactaagt cttctccttc
    34741 tgaaatcgtt agaacagacg tgtcatctgg cagtaccact tgtatatttt taacttgata
    34801 agccaatttc tcccccaaaa cagtcaaaat aaacgtgtct ccgttttcta aacgatctaa
    34861 ttcggtaaat aaccgtcgat taggtaaacc gctgtgagca gagatcactg aatgcgtcga
    34921 atttccacca agcgggaaag aggttccttg caagacgcct gctccatttt caaggatcct
    34981 accattgagc cgatcataaa gtgaaatatt taattggatc ttaggtatat tgatacttcc
    35041 gatcagccat tcctctaatt ggctggatgt tgtactttca tttctgttct tttcattgaa
    35101 aggatcagta ccaggattga atccttggtc agttaatcgt tgttttttct cctccatttt
    35161 ctttcgctgc acttcaattt cagctgcggt ccgtttttgt acttgttcga tacgtttttg
    35221 atcaataaaa ctatttaaag aatccacata aaatggatat aaagcgatca gtaaccctac
    35281 gacgatacat attaataaaa acaaacgaaa caaaagtcga cgagactgtt tttgcttact
    35341 ctgcttcaca tgtgtcctcc cttgcaagtt tttttgttct ttcttttatt atatgggttc
    35401 aggaaaaaaa tagaaaatga aagccgatga ggttgtgaaa aagctgtcct gcatcaagta
    35461 ataagtacag cccaaaaaaa tagctactca tattttttgt tgggttgggc ttattaccgc
    35521 agatgcaagc ttttgaacac cgtttaagga agaggttgtg aaaactgcgt tatgctttga
    35581 gtaataagag cgctagtcag aaaacagctt ctcatatttt ctaggctagt gagcttatta
    35641 ccgagaagca gctgattgaa caccgtttac cgagaggtca tgaaaaagct actttgtttc
    35701 gagtattaag aaaaattctg tttcaaaaca aggagatgca acaaaactta tgtcgcatcc
    35761 tctctcctta gaactttcac ttatgttttt cttgtaaaat tcataaatat tttcatgaat
    35821 tttctaattt cttgcctacc tctcgcatag gatatatgat gaatttatct aaagtttgca
    35881 aaggagttcg acaattgaaa aaatatggcc catttttagc aatttttttc attgctttgg
    35941 catttctcat gcctcattta ttcacacata aactaatttt gggtgctgat gccccattcc
    36001 attacaatcg gttctatgat gccgcgcaac aaattaaata tggggatttc cattattttc
    36061 cttcccttta tggattccag caaagtggca ggatcgtgaa tgccgtttac ggcccatttt
    36121 tcgcttattt acaaggattg atcgtgttgc ttgcaggtag ctggtttcat tatcagattc
    36181 tttcaaattt cattctttat cttatttcag gattttctat gctgatttta ctgagatatg
    36241 tcaatgttcg aaattgggta agtgtaccag tagccgtttt attcatgagt acgtattcta
    36301 ttcaatactg gtctatcaac caaggattca ctagctgggg gacttgtttt tatcctttat
    36361 gtatgatccc tattgctgat ttcgtccttc ataaaaagtt ccctgttatc aaagtcgctg
    36421 tctcagtggg acttatgaca cagatccatt tactgtcttc gatcatgtta gctttgatgt
    36481 atatcccttt ttatctttat tactttttta accagactga aaaaggaaaa gcactgcttc
    36541 aactgattaa gtcggtcttg ctttattttg ttctgacagc aaatatttgg gcaggattag
    36601 ctattgttta cagtgggaat aagattcttc ctccctttgt taacagagaa atgagtcaaa
    36661 aggcgataga tctggatggc agttattggc tccattaccc taaggccttt cctatattga
    36721 tattatcagg ttttttgctt tttattttct ttcaacaaaa gaacacctta ttccaaaaaa
    36781 tcgtgttgta cacaagtctt gttttctttt ttctttctac tagcttgatt ccatggacga
    36841 cattgatcga aaaaaatgtg ccttttatta gtttgatcca gtttccattt cgatttttcg
    36901 tgccatttac gctattgctt ctactttacc ttgccctttc actgaaccag tggtctgaac
    36961 aaaagtggtt ccgtctttta agtatatttg gcattcttat tttttcagca cagacgatcc
    37021 agaacttata ccagcatctg gaaaagtggg aaaatgaaac atttgtcagt cgacatactt
    37081 atctattcga tacgccggaa gaagcaagga aaacattctt cagcaaagat atgtctacca
    37141 gcttgttcgt tttccaaaaa accacacctg actatctgcc aatctataag gagacaaaag
    37201 acaataaata cgaccggtat aacgaagaaa tactagaaaa cgaaacaact tttataaaaa
    37261 cacatccagg cggtcttttg acaatcaagt ggaaaaacac tggggacaaa actgttcata
    37321 tccctgttat cgtctacgat cggactgtgc tacagcaaaa tggaaaaaca ttgactgatt
    37381 atgaagtaac agatatcggg accccaatcg tgaagcagaa aaaaggaatc aacgagctca
    37441 cgcttcatta tcaaacgcca atctatttct atttcattct ttctcttact ttgattggct
    37501 ggtttacctt gctatgttta tttatttatc atagatacaa gcttttacgt gcttaggcaa
    37561 aagggataaa tacatccgat caaaaaactc tccaaacggt ataatcgttt ggagagtttt
    37621 attttaatca gtttttttat gttatatgtt atatttttta ttcgttcaca tctaattcga
    37681 tttccacccc gtagtgatca ctgatgattg gactatcgat cccatcaaaa atccgttgat
    37741 attttttaac ctgcattcct tcagggacga aaataaaatc tatacgcaat ttttctgtat
    37801 tttgttccca accatctatt ttcttttcca cagtagctgc ttttcctttt tcattcgcga
    37861 caacatacga atcttgaaga agaaggcaac tttctcttac agtataataa ccttgagagt
    37921 ctactggatt attgaaatca cctaggaaaa aaagtggtac gcgagtttcc aaaaagtagt
    37981 tttccaagtt tttccactca aaataaaagc cacttttttt atctgtccac caagaaaagt
    38041 ggcagctcga gacaaaaatg tcctgatcat ctatctttgt tttcccaatc aagatcttgc
    38101 gtgtctgata atcttctttc ttttgagatt gggagacggt aaggacctga ctttccaatg
    38161 tttttttcgt aagcagtgca tttccttctt catatttatc ataaccgatg tggctcattt
    38221 cccaactcca aaaatactct tttccgtgtt cctttaaaag ctgaactaaa cggtaggcaa
    38281 aattatcttt acggataggt acttgattct ttacctgaca gaacttcatt agcttcgcag
    38341 gttctacttc ttcagattcc agtaattggt tgatttcttg taaagcaatc acttcataat
    38401 cttctaacag gatcttatct gctaattctt gcaatttttt ctctggttct ggctcaagcc
    38461 agctatgcgt gttgagtgtc atgaatttca ttggttttct ccttgtcttc atttcgaaac
    38521 aaaagggttg tgacaaaatt tttgtcacaa cctctccagc aaatagtgtt ctcctgctaa
    38581 ctcctcagta ataagctcaa tggaccagga gctatttttt gtttggctgt ttttatcact
    38641 tggtgcaggg cagctttttc acgacctctc cactaaacag tattcaaact gctgcttctc
    38701 ggtaataagc ccaaccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatg
    38761 acttgatgca ggacagcttt ttcacaacct ctccttacat tttaatggag ccgatcactt
    38821 taccagctgt cactgtacct aattgcttga tcactacttc ttctacttta tcgtttgtaa
    38881 atacgatcat gatcgttgga tctttttctt cgttttggat cacatcaaga tccatctcag
    38941 ctaatttcgt tcctgttcct acttcttggc cttctttgac agcaatctca aaagctggct
    39001 gattcatttg gactgtatcg attcccatat ggatcaaaac ttcgatacca tctttcgttt
    39061 ccagcccaat tgcatgtttg ctagggaaaa tacttgtcac ttttccagcg atcggcgcaa
    39121 caacttctct tgatgttgga ataacagcaa agccgtcacc catcattttt tgtgagaata
    39181 ccggatcttt tacttcagat aaaggaatca cttctccaga agctgcggaa actagttctt
    39241 gttctatacc taagtattgc ttactacctg cttgtccatt tcctgtagaa accatttctg
    39301 gctctggaat ctctgctcct gattgcagca gatcttcgat atctgatttc aagacatctg
    39361 cttttggtcc ataaactgct tggactccat tgtctttgac gatcaatccc atcgcccctg
    39421 ctcgtttcca ggcttcttca gtccctactt tttctcgatc atttacactt acgcgtaacc
    39481 gagtcataca agcatcgaca tctttgatat tttcttttcc tcccagcaaa tgaatgatct
    39541 ggaccacttg ctgatctgct gttcctgttc cgctgttaga tacaacggtt tcttcactgt
    39601 cattgtcgta atttccgtta cgtcccggtg tagcaaagtt gaattttttg atcaagaaat
    39661 ttgctaagaa atacgtaaca acaccaaaag cgatcacaca caacacgaag ttgataagat
    39721 ctccacctag acccgctttg atagctaacg gtgtccgagt cagcaattcg atattcccaa
    39781 atgaatggac ccgcaatggc aagatatctg ccatcgcaaa tgcagcccct tggatcactg
    39841 aataaatgac atataatggc acagcagcaa acataaacat gaactccaat ggttctgtta
    39901 cacctgttag aaagacagcc aacgctgctg aaaaatacat tgatttatat cttgctttct
    39961 tatcaggatc gacattacga tacatcgcaa aagccattcc catcaagata cctgaagaac
    40021 caatcatttg tcctactttg aaacgagccg gcgtccagtt atgcaacaca aactcatatt
    40081 gcgacatatc ccctgcacct ttaagattca ctaaatcagt ggcccaagcc aaccataacg
    40141 gatcttgccc aaatacttgt gtccccgcct gtgcgcctga taaaatctca tacgatccgc
    40201 caagctgtgt atagttgata ggaatcgtca gcatatgatg caatccaaat ggcaatagta
    40261 aacgttctaa tgttccaaat aaaaacggtg ctagaatcgg tgcgctttct tgtgattctg
    40321 caatccataa accaaaatta ttgattccag cctgtacgtt tggccacacc acagcaagaa
    40381 tcaatgccac gattgttgac cataagataa caacaaacgg aacaaagcgc tttccattga
    40441 aaaatgacaa agcatccggc aattttctat aattatagta cttattataa gccattgccc
    40501 cgacaaatcc agcgatgatc cctacgaata ctcccatatt caaagctggt gcttccaata
    40561 cacttgtaaa aaatccttta accatgatct tcgtaccgaa cagagtgtgg gtaaatgcat
    40621 cttcattcgc aagcatgtca cttgtgacac caaagatagc cccagtgatc cgattgatta
    40681 aaataaatga gatcccagct gcaaatgctc cgcctgctct ttcttttgcc caacttcctc
    40741 ctattgctaa agcaaataaa atatgaagat taccaataat tgcccaacca atattttcaa
    40801 ttactccgcc cgttgtgaca agcggcgtcc aatcaggatt gatcaaggga attgatttcc
    40861 ctatactgat catcaaccca gctgccggca taactgctac cacaaccatt aaagcctttc
    40921 caaatttttg ccaaaactca aaactaaata tctttttcat tccgtttagc tcctttacgc
    40981 aatcggtttc gttaaacgta ttatagcgtg cgttttcttt tttgtaaatg ctttattttt
    41041 tatttttctg tctataccat tctataaaac ctataatacg tgtgttccct tctataattt
    41101 tctcctaaaa atttattttt ttaagctttt ttctatgaaa tattttcttt tgtctattga
    41161 cttgtgcaat cggttgcgtt tattatgaac gtataaaaca gctcattagg aggagaaaaa
    41221 tgaaacagat caaacgacta tttgctattg atccatggaa aatcagcacg acacaattag
    41281 ataaaaaaaa tcttcgactg caagaatctt taacaagtat tggaaacggt tatatgggga
    41341 tgcgcgggaa ctttgaagaa acttactcag gggaccacca tcaaggtact tatttggcgg
    41401 gtgtctggta tcccgataag acacgtgtcg gctggtggaa aaatggttat ccagaatact
    41461 ttggaaaagt gatcaacgcc ttgaacttta tcgcgataga tatttcgatc aacgatcagc
    41521 tgatcgactt ggcttctttg gaaccagaag acttttactg ggaattagat atgaaaaatg
    41581 gtgtattatc tcgtacattc acaatcacta cttctactaa taaagtacgc atttccttcg
    41641 aacgttttct gagcattgtc aaaaaagaat cagcttatat ctatttaact atagaaatgc
    41701 ttgaaggtag cggaacagtc aaactcgtat caaaattgga tggaaatgtt caaaacgaag
    41761 acagcaacta tgatgaacat ttttggaatg agattgatcg tggaactata gaagaaatcg
    41821 gctatttgac gactaaaact attccaaaca actttgggat tgaacaattt actgttactg
    41881 cagcaatgcg ccatttcgtt aatggaaaat cagtcatccc tgactatcac gaagatactt
    41941 tagcattaca tggttgcttg tctgcagaat tatctcaagg tgagacatgg tcagtggaaa
    42001 aagaaattat cgttcttact agtagagata ttccagaagc gcagcaagca gaaaaagctg
    42061 tctcagaatt aacgaaccag acaaacaatt atatccaagc aaaagaagag caaacagcgg
    42121 cttgggcaga acgatggaag cttgccgatg tggtcatcga aggagacgaa gaagcacaac
    42181 aaggtattcg attcaatttg ttccaactat tctctactta ctacggcgaa gatgaacgat
    42241 tgaatatcgg ccccaaagga ttcacaggag aaaaatacgg cggagccact tactgggata
    42301 cagaagcgta tgctgtccct ctttatcttg ctttagccaa acctgaagta actaagaatc
    42361 tgctgaaata tcggcataat caactgccgc aagcaatcca caacgctcag caacaaggat
    42421 taaaaggcgc gctttatcct atggtcacct tcactggtgt cgaatgccat aacgagtggg
    42481 aaatcacatt cgaggagatt caccgaaatg gcgcgattgc ctatgcaatc tacaattata
    42541 cgaattacac cggcgatacg gaatatttga aaaatgaagg actggaagtt ttagcagaaa
    42601 tcgcacgctt ctgggcagac cgtgtccatt tttcaaaacg agcaaataaa tacatgatcc
    42661 acggtgtgac cggcccaaac gaatatgaga acaatatcaa taacaattgg tatacaaata
    42721 cgatcgcagc ttgggtcttg cgttacacgc gagaaaatta tttaacttat caaaaagaag
    42781 ctgctatcac tatcagcgaa gaagaactag ccaaatggaa agatatcgca gagaacatgt
    42841 acttcccagt tgacgaagaa ttaggaatct ttgtccagca tgacacattc ttagataaag
    42901 atctcatgcc tgtatccgat attcccacaa gcgatcttcc tctgaatcag cattggtctt
    42961 gggacaaaat ccttagatct tgcttcatca agcaggcaga tgtactacag gggatttatt
    43021 tcttcaatga tacttttaca aaagaagaaa agcaaaaaaa ctttgatttc tatgaaccaa
    43081 tgactgtcca tgagtcttcc ctttcgccaa gtatccattc gattttagca gcagaattag
    43141 gaatggaaag caaagctgta gaaatgtacc agcgaacagc tcgcttggat ttggataatt
    43201 acaataacga cacagaagac ggcttgcaca tcacttccat gactggtagc tggttgtcga
    43261 ttgtccaagg atttgctcaa atgaaaactg cccatgatca gttgagattc gcaccatttc
    43321 tgccaaaaac ttggaaagct tattctttcc atatcaatta tcgcggacgt ttattgttca
    43381 tctcagtcac agatgaacaa gttactttcg agttattaga aggttttcct ctagaaattt
    43441 acgtctatga acagtcttat tctttagcgg atcgtctaga aataacgcta agaaaggagg
    43501 aagcaaatgt ttaaaggggt attgtttgat ttagacggtg tcattaccga tactgctgaa
    43561 ttccattatc gtgcttggcg aaagcttggt caggaaatcg gcattacgat cgaccacaca
    43621 ttcaacgaac agctaaaagg cgtcagccga gaagactcac tctctttgct tttagcttat
    43681 ggcggaaaag aacactcttt ttcaaaggaa gaatttgcag aattagcgaa aagaaaaaac
    43741 gattattatt tagagatgat ccagacgatc gaaccaaaag atgtcttccc cggggtcgtg
    43801 cctttactgg attctttaaa agaagaaaaa atcaagattg ctcttgcttc tgcaagtaaa
    43861 aacgggccat tcctacttga aaaaatgggt cttacacctt actttgatgc tattgccgac
    43921 ccggcagaag caacaaacgg aaaacctgcg ccagatatct tcctccttgc agcaaaagct
    43981 gtcggactaa aagcagaaga atgtatcggg atcgaagacg cgcaagccgg tatccaagcc
    44041 atcctctcaa gtggcgctca accaattggc gtgggacgtg ctgaagacct tggagaggag
    44101 atcccgcttg tccctgatac gacttttctt acgatcgatt acttgaaaga aaagtgtcat
    44161 gaccatggat aatccagaga tctccgtcgt accagttcca gagactgaaa taaagaaaat
    44221 cacattgaag aacgactact tagaagtcgt tcttctcaat tacggtgccc gcctgcacca
    44281 aatcttcgca ccagacaaag aaggaaaatc agaaaatgtt ttattgtctt acgatcgttt
    44341 cgaagatgtt ctaacagaca aaagcttttt tggagcaact gtcggacctg tagccggaag
    44401 gatccgggat gcttcttggg gtgagcatag attggagaaa aactgcggat cacaccatat
    44461 ccacggtggc acgaaaggct ggtctttcca attttgggaa gtagaagttt tcaaaactcc
    44521 atcatcaata ggtgtggtct tttacttaaa agatgaattt tctacctatc caggaccaat
    44581 cactgctacg atcacttacc gtttaacaaa aaacgaatta gagatgacaa cctctgctag
    44641 tagccttgtt gaaaccatct gcaatccgac caaccatgcc tacttcaacc tttcaggaaa
    44701 tggaacaaga gacatctatg aacatcagtt aacggtcttt ttagatggtg tgcttgaatt
    44761 agatcaagaa aaactgccta ctggaaattg gacaaaaaaa gaaaaactgc caatcgactt
    44821 tagaaaatcc ccaacactgc aagagatcct tgcatgttat ccagatggac tagatgatgt
    44881 atttcttttg catcatccta ggctttccaa aacttcctta caattattcg agaagcattc
    44941 tggtcgccaa atgactatag caacttctaa taaaagtatg gtattatttt ctacgactgg
    45001 attcgaagct gatttctctg tcaatggaaa acagatgcat tcaaactacg gattagcaat
    45061 cgaaccgcaa gaaatccctg atatcgttca tttccccaaa tttggatcga tcaacctcca
    45121 tcctggtcaa gaacggatca gtcagacgat ttatcggttc tctgcacaat aacaagagaa
    45181 aggtgttttt atcatggcta ttaccgtaaa agatgtggcg aaaaaagccg gtgttgccac
    45241 ctcgaccgtc tctcgtgtca tcaacgatca tcctagtatt tccgagacaa caaagaagaa
    45301 agtccgaaaa attatggatg aactaggtta tgttcctaac atgactgccc gcaatctagg
    45361 aaaacgcatt tccagtgcca tcggcgtaat cttaccccct cttgattcaa aagagcggat
    45421 cggtaaccca ttttatcttg agatcatgga agcaatcaac gaagaagcgc ggaattatga
    45481 tatgactaca gcaatcgcta ctgctaaaaa ttttgaattg ctgttagaga acgtccagcg
    45541 tatgcattta caaaaacaag tagatggttt tattctagtg tattcagata gtcaagatcc
    45601 tgtcattgat tatttatacc aaaaccacat ccctttcact ttgatcggac agccttatca
    45661 tcatgagaac gaaatcgttt atgtagataa tgataaccag cttctaggta aacaagcaac
    45721 agaatttttg atccagaatg gacataagaa tatcctgttt gtgacaaaca cgactcatga
    45781 aagtttgtat ttcgaacgat atttcggtta tcaaaaagca atgatgatgg ctggtcttcc
    45841 tgcgttatcc agtgtagtac tagagcatcc ggaggattat ctgtcctttg agaaaacgtt
    45901 gaaagaaaac gaagccacag cattggtggt cattgacgat atcttcgccc ttcgcacgat
    45961 gcagttggcg caaatgtacg gctatgtcat tccagagaca ctctccgtca ttagcttcaa
    46021 caactcgatc ttctcaacat taacacaccc ttaccttaca agtatcgata tcgatatctc
    46081 cgaattagga cgtgtcgcta tgcaaaagct gcgcgaattg atccatgaag aattgagcag
    46141 tggcgtacgt ttggtcatcc ctcatcgctt gatcaaaaga gagacagtcg ttcctttaga
    46201 aaacccatac taaatagctg actcactaaa tacgctgacc atttatccta tggtcagctt
    46261 tttatgtaaa agaagatact acttctaaca cattgtttat ctgtaaaaaa gagcgtggga
    46321 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag
    46381 caactccttc ggaaataagc tgaaattcac aaaaatttaa aaagcaattt tcttgagttt
    46441 cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttcctt ccttcaattt
    46501 tttacacgtc gacgattcaa taaaaaaccg cctaatattg taaagacagg tgcaagaatc
    46561 gagaaaaaga cgaatggcaa gtaatgcaac gcgctaactt tcatcgttcc cataatgaat
    46621 gtgccgctga ctccccacgg tacgagagaa ttgacggtcg cacctgaatc cgctagtgtt
    46681 cgtgtcaaat aggtttttga taagccaacg tcatcaaaag aacgcttgaa tgtttctcct
    46741 ggaagaatga tggacagata ctgttctcca acgatcagat tgattccgat acagctcaag
    46801 gcagtaaacc cgactaaacg agaaggtcgg tccatctttt cttttaattc ttgaacgatg
    46861 gtatcaacga tcagatattt gatcagtaac ccacctaatg ccaatgcaag taaaatcaaa
    46921 ctagcagaac ctaacatact catgatcccg cctcgagaaa gcagttcatc tagctttgga
    46981 tcaggtgtat ttgcgacata accattcatc aagatgtctg ctgcttttcc gaccgtcaaa
    47041 cttttctcat ggataaaaga aagaacgaga gcagctgtcg atcccgctag taatgtaggg
    47101 atagccggaa tttttttgat tgccccccca aataaaataa ctagtggaat caatgtccaa
    47161 ggagagatcc aaaagctcgc tttcatgttt tggatcattt cattgatact agctagatcg
    47221 gctgaatgac tgttatgtcc taatatcgta taaaaaatca acgaaaggac gaatgctggg
    47281 aaatccgtat aacgcatacc agcgatatgt tcgaatagat taactcctcc aatacccgct
    47341 gccaagttgg tcgtatctga taaaggcgag cagttattcc ctaaaaaggc accggaaacg
    47401 actgcgcctg tagtcattgc atcattgaat ccaagtaaat gaccgatccc caaaaaagca
    47461 attccaatcg tcgaaatcgt tgtaaatgaa ctgccgacag tcactccgac aattgcacaa
    47521 acaagaaatg ctgtcggtaa aaagaaacgc acagaaacaa gtttaaatcc ataaaccata
    47581 atcgtaggga tggttccaga taaaatccat gaagcaacta agacaccaat caaaagaaaa
    47641 atcaggattg gaatgatccc tggagtgatt cctttgatga tcccgtcatg gatctcatcc
    47701 catgtgaact ttttaaatct gccataaaac aacaaacaca tgaaagctgc aaggatcggg
    47761 atatgtggcg agagctggaa tccgatgatc agtactccta ggagtgccaa caacacgatc
    47821 aatacagcga tactttcttt taaagaaata tttttattca tcgtcgatct cctatttcaa
    47881 atgaaaaggc cctgccatga aaaaactatt cacttggcag gacctcctac taaatcagcg
    47941 acaaaacgtc ggctgaaaga cttatttttt ttatttttct tctaaacttt tttctacctc
    48001 aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac
    48061 tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac
    48121 gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt
    48181 tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga
    48241 aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa
    48301 gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat
    48361 tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa
    48421 ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac
    48481 ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat
    48541 cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa
    48601 ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa
    48661 aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa
    48721 acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa
    48781 gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc
    48841 tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat
    48901 gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat
    48961 atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac
    49021 gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa
    49081 agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac
    49141 aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg
    49201 aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc
    49261 aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac
    49321 cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc
    49381 agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa
    49441 gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct ttgagtctat
    49501 tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc
    49561 cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa
    49621 catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc
    49681 aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg
    49741 ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat
    49801 tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg
    49861 atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat
    49921 gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct
    49981 ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg
    50041 atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca
    50101 ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg
    50161 tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc
    50221 ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata
    50281 acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc
    50341 attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt
    50401 acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct
    50461 tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat
    50521 ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga
    50581 cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca
    50641 ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt
    50701 tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg
    50761 ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat
    50821 tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt
    50881 ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc
    50941 caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat
    51001 ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg
    51061 tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc
    51121 atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca
    51181 aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt
    51241 ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca
    51301 tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata
    51361 ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca
    51421 ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt
    51481 aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa
    51541 attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga
    51601 tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt
    51661 aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat taacgacaac
    51721 cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt
    51781 caaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa tttttcttac
    51841 cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg acaaaattta
    51901 aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct tcacgtgctt
    51961 gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt tcaggtgtgc
    52021 ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc gcatttagtt
    52081 ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc aagtcttctt
    52141 cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca cgtttctcag
    52201 gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag aaagtaaaga
    52261 atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg tagaactcaa
    52321 atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg
    52381 ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctact tctggtcctt
    52441 ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct gctccaccga
    52501 tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat tgcagaattt
    52561 ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg atatcttttg
    52621 ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca agtaaagaga
    52681 tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt
    52741 ttctcatttg attggaaagt gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca
    52801 cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa
    52861 tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga atcaaatgtc
    52921 cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg ctcttttctt
    52981 caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta
    53041 attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa agcaggaacc
    53101 tgctagcatg tgtaattaat tatttcttca ggataggata ggaaataaaa aaagtccctt
    53161 ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt ctagatatcc
    53221 aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag actcctgaaa
    53281 tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct gaaacattgg
    53341 ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg tctatcatag
    53401 agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc tcattttttc
    53461 ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa aagaaataac
    53521 tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct cttattcttt
    53581 cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat
    53641 ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat
    53701 tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa
    53761 aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc
    53821 aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag
    53881 taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca
    53941 actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt
    54001 ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac
    54061 tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa
    54121 tttaaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct
    54181 acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat
    54241 ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt
    54301 ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc
    54361 ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac
    54421 aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa
    54481 aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg
    54541 gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt
    54601 aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat
    54661 tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca
    54721 aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac
    54781 accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg
    54841 cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt
    54901 ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaacc
    54961 aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt atttcttctt
    55021 cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa gcagttttgc
    55081 gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga tcaagaactc
    55141 atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa gatcaagcat
    55201 gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt ttttttcttt
    55261 ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat tgagataacg
    55321 gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa
    55381 cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat aaatcagccc
    55441 ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt tctcagctac
    55501 cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa aagctgcttg
    55561 taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt gtagagcctc
    55621 ccgtaatttt aaacttttat aatatatata gttcaaattt attctgaggt ttattataac
    55681 tatctattga gaaagtatca agctagcaat tccttgttta tgaattcatt gacagaaatt
    55741 tataaaaaga atattatgta ctaaaataat aaaaaaagtt ttataaaaag gtataaaacc
    55801 tttttattaa actatttaat cttgtttagt atatattttt agaaaaagga gtggggtttt
    55861 tatggcagaa atcgtcaaag tcacacattt gaaaaagagc tttggtcgtt tcgaagcttt
    55921 aaaagatgtc tctttcaccg tacattcagg tgaagtcgtt ggatttatcg gaccaaatgg
    55981 ttctggaaaa tccacgacga ttcggacact tttagggatt ataaaaaaag acggcggaac
    56041 ggcaacgatt tttgggcaag atgtatggga agatagttta tctatccatg aacgtctttc
    56101 ttatgttcca ggcgatatct ctttgtgggg aaatttgact ggaggagcga tcatcgatct
    56161 cttcatgaag cttcatggaa aagggagtgc tcaaaaaaaa gatgaactga ttcaacgttt
    56221 ctctcttgat cctaagaaaa aagcaaaaaa ctattcaaaa ggaaatcgcc aaaaagtggg
    56281 cttgattgct gctttggctg ttgattcgga tctctattta tttgatgagc ctacttctgg
    56341 tttagatcct ttgatggaaa cgattttcca agaagaagtt gaaaaattga aagaacaggg
    56401 caaatccatc ctactttctt ctcatattct cagtgaagtc gagcgactag cggataaagt
    56461 cgtgatcata cgcgaaggaa cagtcgtaga agaaggaaca ctcgatgaat tgcgtcattt
    56521 gactcgttcg accatcacta tagaaacaga agcagagaat agtcacttag cacaattgaa
    56581 cggtgtccat gactttgtgc aaaaaggtca cctcgctcaa tttgctgctg ataacgaagc
    56641 tttcgatgtg attttagctg aagcgtccaa attaggtgtg aaaaaatttg aatctattcc
    56701 accgactctt gaggatttat tcatgagaca ttatgaagga tgaggaggaa gcgaaatgac
    56761 tgataaattt tcacgctggt ccaccctttt ttctcaaaac cttagccgtg actggaaaaa
    56821 gatcctgata tggctagtcg gtgtcactgc tttttctggt ggttttgtcc ctgcgtttga
    56881 agaattgact tccggacaag gattagctgg attatacgaa accttgcaaa acccggcaat
    56941 gattgcaatg gtcggaccaa ctccagcttc gaatgctgct gagtatactt taggagcgat
    57001 gtatgcaaat gaaatgttgc ttttttgcag tttgtttgct gctgtgatct ctgttttaca
    57061 cgtggtgagc caaacgagaa aaaaagaaga acttggtctg attgaactta ttcgttcctt
    57121 tcgagtcgga cgtcaagcaa actctttagc tgtcatcgct gaaagtattc tgatcaattt
    57181 gcttttagca atatcgatca gctgtacgat gatttttttc caagcagatt cgatcactgc
    57241 agaaggtgct tggctttttg ggctatctgt aggtagtatg gggattttgg ggagtgccgt
    57301 tgcactgttt gctgcacaac tgatgccaag ttcagccagt tcgaatggtc tgtctttagc
    57361 gtttttaggt ggactttacc ttctacgagc aataacagat gtagctgatt cctccctttc
    57421 gatgtggaat cctttaggct ggtcttattt gacctatcca tttacaacta acaactggat
    57481 tcctttgctt tatttgtttt tgttcagtct aatcttgata atcagttcat ttgttttaga
    57541 ggaacatcga gatttgaaca ccggctatat ccccgaacgt gaaggaaaac cgtatgctaa
    57601 acgttctctt ttatccttgc gtggtttatt attctatcta aataaaggga tgacaatcag
    57661 ttggcttatc acttttggtt tgatgggggc ggcatacggc tctatctacg ggaatatgca
    57721 acagttttta gaaagtaacg acttaatgaa acagatgttt atccaatctg gcgtctcaat
    57781 tgaaaaaaac tttacaagca cgatcatggt cgtcttgatt tgtctagctg ctattttacc
    57841 aattgctagc atcaatcgtt tatttacaga agaaacccac ggacgtatga atcagctgtt
    57901 cgcaacaaaa atttctagga accaacttta ttggaccaat atgttactag ctattctatt
    57961 tgctatattg gcaatcctga ttagttctgg tgcactaggg ctttctgctt tggcttcgat
    58021 gaaggatgcg aaagacatgg atctattgat gtttcttaaa gccgggttca atttcctgcc
    58081 tgccgtttta gtgtttatcg gaattagtgg ctttctattc ggatggcttc ctcgaatggg
    58141 taaacttgct tatatctact tagcttactc ctttgcactc aattactttg gcggaatttt
    58201 ggatttacca gaatggtttt ccaaaacagc tgtccctagc tgggttccac ggatgccaaa
    58261 cgattctttt gatctttccg tgttccttac gttatcatgt atcagccttg cactgttcgt
    58321 ccttggttct attggttata ggcgaagaga cttactagaa ggataataaa aaagcatgta
    58381 caatttcatg ctagcctcca aatctgggtc aaaaatccaa catttggagg tcattttttt
    58441 atttacaagt cttctaactg ctctttatca atgatccaga cttctcgatt ctttcttttt
    58501 acataaccat tctcctctaa taatttaaat tttctagata aagtttcggg ggtcgtaccg
    58561 ataaatgcag caagttcttt cattttcatt ggcaggaaaa agtgatcctt ccctgtcgct
    58621 ttagctaagt caagcagata gacagcgact cgttcttcta ctttttccat catcaaaaaa
    58681 gttgtctgct gctgtacttt tgttaatttc tcaacgttga ttgctaaaag tttcagacta
    58741 agggcgggat aatgcagtaa gagttccgag aaatcttttt gtctcaatat gcaaatttcc
    58801 gttttttcta gtgcttcgcc aaataaagac tcatttggaa cgcctagcaa ctggctctct
    58861 ccctcatatc cgcccggctc tattactcgt aaaagctgtt cctttccgct agcagataat
    58921 tgatacactt tcatactgcc ggaggccacg atcactaatt gaggttctcc attcggcgca
    58981 agaatctgtt ctcctttttc cacatagtga tgtttgacaa gtgcgtgtac tttttcttga
    59041 tcttgctgta ctaagtgatt gaacaaagga acaaatgaaa cacataaatg atcatgactc
    59101 ataatctctc ctccttctaa acagctattt ctaataaaga aacataactt cccttattcc
    59161 aactgataat tttcttttat catagcataa acgtcaagcg aaccagatac cagcaaatag
    59221 agtggatttc tttttgtccc ttgctttatt ttccttcttt tgagacctta agtaattgat
    59281 tcctatcaat tttttaaata tttacttttt gaataatcaa aaacaaacct tcactcgcaa
    59341 gccgagagta aggtttgttt ttcgcattgc ttatttcgtg ttgtctattg aaatttttcc
    59401 gcacctagct tttgatatag tggtgctagt tcttgtaagg tttcagcggt tatttgctca
    59461 acgtgttcga tttcttgcgg atagaagaat agtttaccca tttgaaaggt tcggtctctc
    59521 atcgcttttt tcaactcttc ttgtttcttt gtatcggaca aatagtcaga caaagctaag
    59581 tggtcgtcaa gttcatgttc tggttgcggc catatgtaat aatccttgat atcgactgtt
    59641 ttgctccact ctttcagtat cgcaagcaac tggttatacg ctgcaatgct accatatcgt
    59701 tcttcgcttt tataatgttg gtacatcaaa tagatttggt attgtttttg attcaacaaa
    59761 actgctagac aggcattctc gtcttgacga gattcgcttc ggtacgcgct ccaaaaatga
    59821 ctgcgtaaat tccagccatt cgtccagctt tcgattttag gtttgctcat gccgtacata
    59881 tcaggcaaaa gtgcagctgt ctggagctga atctctttcc atttttgcca gtgttcttta
    59941 tattctttct tgatttgttc aatcttatct gttggatggc tctctttgag ctgtttgaat
    60001 gcgtagattg gtcgatcaaa caactgataa tctgattttc caagttccat tgtttcgcct
    60061 ctttccgtta tcttttgtat tctacctgaa ttttgcgata tggacaaata gaggttgtga
    60121 aaaagctgtt ctgcatcaag tcataagaac agccaaacaa aaaactgcga tgagcatcac
    60181 cgcagtttta ttgaacttaa ggttgattga tcacatctaa aagcgttgaa aataaaatgg
    60241 cactagaagc agctcctggg tcgatatgtc cgattgcacg ctctcctaga taggaagctc
    60301 gtcctttttt agctaccaag actttggtgt gttcttttgc ctggtcaatg atttcttttg
    60361 ttaaattccc tgccttcaat gcctctgtga ctggcagcca aacatctaac atcgttttat
    60421 cgtcagcttc tgcctttcct cggctttgaa tacccgctgt tccctgctcg atcacttcgc
    60481 ctaatttttc ttgagaatca atagaatcga cctcttttgt cgctttcatc atgttcatga
    60541 atgcagatcc gtataacgga ccagaagccc caccgacttt cgatatcatc gccatagaga
    60601 atactttgaa tgtatcgctg attgtttcag gatgatcggc ttgaaaagct tcttcataag
    60661 ctgaaacccc tcttcccatg ttatttccat ggtctccatc gccaatcggg gtatctagat
    60721 cactgagata ttgtttgttc tgattgattg cctctgcaaa ttgcgtgagc cattcttgga
    60781 tttcttgaac ggttagtttc atattatcac ccttttatct tgagattttt taccatgaaa
    60841 tcgtctgtac attgctttct aaagaatctt tccattgtgt ctccgtcaag tcgatcagtg
    60901 ttagagaaag accttgcata tctagtgagg tcataaaatt accgactttt cggaaaacta
    60961 cattgacgcc tctttcttcc aacagagcta aaacatcatt catgaacacg aactgttcca
    61021 tcaaaggcgt cccgcccatt ccgttgacta gtacacctgc ttcttttggt aaattcgagt
    61081 aatcatctaa aatcttagta acaagttcct ttgccagtac tttagaaggc tgcatctttt
    61141 ctcgacgata gcctggctca ccgtggatac caacaccata ctcgatctca tcttctccta
    61201 gttcgaatcc tggtttccct acttctggta cagttgctgc tcgaagcgct agaccgatcg
    61261 tcttagttgc ctgtacgacc ttttctccca gttctttcaa ctcagctagg gaagcaccgt
    61321 ttcttgctgc atcgcccacg attttgtgta caaggacagt acctgcaact ccgcgttttc
    61381 cagcagtata cgtactattt tcaacggcga tatcatcatc tacgatcaca ctttctactt
    61441 caatatcgtc catagcagct aattcctttg ccatatcgaa gttcaatgca tcccctgtat
    61501 aattcttaac gatcagcaaa actccttttc cactatccgc ttccttaata gccgtctgaa
    61561 tctgatctgg agttggagaa gtaaacacat caccaagcac cgctgcactt aacatccctt
    61621 cccctacaaa cccggcatgg gatggttcat gtccgcttcc tccaccagaa accaaaccaa
    61681 cttgttccgc tttctgattc ttggctacaa cacgggaaga ttctacacga tgaacaagtt
    61741 ctggatagct ttttactaat ccttccagca tttcttcgac aatattgctt ggatcattca
    61801 tgattttttt caatttattc ccatcctttc tttccggcat tttgtgcttc tgttaagatt
    61861 tgctgagtat catctgttat accggcagtg atggctgctg caaaagctcc ttccactaaa
    61921 ggcgcatcca ccagatgata acgttgctgc tgctcttctt ccaacatgtc ttttgccagt
    61981 tccgagttca ataccgcgct accgatatcc gcaaaaacaa aataactagc tgcatcgctt
    62041 gattcattga ctgcttctac gattttcatc gggtctgatc cgatctcgcc atttattccg
    62101 ccgagagaaa aaatttgcac ctcttcggat ttttgcatct gctcaatcat ttctttaata
    62161 ccatctgtta ctttttgact atgagaaata agcaaaatac tttttttcac aagaatcttc
    62221 ctttctttta catagttgct aacgattggt ttcgctttca ttaatgaaca attcaagtat
    62281 ataataaatt tctaattttg ggaagatatg tgtagtgctt cttgaagttt gttatacttt
    62341 aattaaaaga gaggagtgat cgaatgatta caacaacaac gaacagtgta gagggaaaag
    62401 ccgtagtagc ttataaaggg atcgtttttg gtgaggtcat tactgggatc catgcattta
    62461 aagacttgga ggcggggttg cgaaatatct ttggcggccg ctcaaaaagc tatgaaaacg
    62521 aactaatggg tgcaagagaa gaagcacttg ccgaaatggc cgaacgtgca aaagcattag
    62581 gagctgatgc gatcattggc gtaaaaatgg actatgaagt attaggcagc gacaatggca
    62641 tgctgatggt cacatgtagt ggaacagctg tctccacgag ataagtaggg gacatctgtt
    62701 ttaataacta aagagagatg ggacatgtag tggcactcaa gaattagatt tttcagtcta
    62761 acttcgaggt actctacctt cccatcctct cttttttatt tctttttgcg gatgattccg
    62821 taagctgctg cgactaacac acccgcccct gctaaggaat aactgacaga agctgttttt
    62881 tcatttgttt ttggcaaagt ctgacttgtt gatttggatg gctctgtcgt ttgctgaact
    62941 gtttctgtcg tgcttccaga ggtcactgat ttttctcctg ccgaatgcaa aatatctttt
    63001 attcgttgga aactagctgc taagtcagct ttttttgctt caaaatcatt agcaactgtt
    63061 tgtgcttcgc tgatctttcc gtggattgct tgactggccg ctgtgatatc cgcttgagtt
    63121 tgacttagct ttccagaagc tgtttgagca acctgattta attgctgatt gaattctttt
    63181 gcttgttgga tcttcccgtc aagatctgtt tggacagctt ccgcttttgc tttcgcttcg
    63241 gcaagctttt tctcaagttg tgcacggtca tttcccactt tatttttaag atcagctaaa
    63301 tcttgatcga ttgctgcttg gtcttctttc aatttgtttt ttgcagcttc taatgcttct
    63361 tcgagttctt ttcttttatt ttccaagtct tgcaaatgat cgatttggtc agaagaggta
    63421 gaactattta ttgatgtatc ttctgaagat gaaatcgttt ctcctgaatt atctgtcggt
    63481 acattggaag tcatttctgt ttccgaggca ccgttttcag caccagaaga gcttatagtt
    63541 gttgaaggtt ctgtttgagc aggttcagaa gtttccgcaa aagctgtctg tcccaataag
    63601 aaaatactgg aaacagcaat tggccagatt aacggcttca ttcttttttt catcactttt
    63661 cccatcctta ctacttttta actaatttct tattataagc aaaaatgttt ggaagaacca
    63721 taaacacaac aggctgtttg tctaaaaaac ggtgcatctt ttattttttt gtacgttccg
    63781 ggcttttcca tgtcccgctt ccataaccta aaatcgtttt gactgctgga atgaaagtcg
    63841 tcacgatcgt tatcgcgttc actatccaga acaaaagcat atatagtggt gcgaaaagaa
    63901 ggtatttcaa ttttcttcgt cgatcatcta caatcagcga agctagcaac tggaaaaatc
    63961 ctgcgaccat ttcaaagcag atgaacacaa aactgaatgt aaaagtaata taaatctgtt
    64021 cgtagtttcc ttgatataca taatgcccga ttaatccagc aaaaagaact acggaaatac
    64081 aaaagaatat tgaccagaca atactcaatg tttgatcaat gaagatgatc gttcttccaa
    64141 tatgtttaaa aggatggagc atcacttttt tgaaattcgt cagccagact tctgttccac
    64201 cttttgccca acgttttctt tgtcgatata gcatcttcaa cgtcacaggt acttccatga
    64261 aaaagataat ctcaggagca aaaacagata accagtcatt taactgatga tcccaagcaa
    64321 tactgatgtc ttctgtcgct cgatcttgtc tgaatccgcc cacatcgatc aaggcttctt
    64381 ttcgatacat cgtatttgct ccactgtacg cataaagacc accgaaaaca gctgactgtg
    64441 ttcgtttaat gatgcccacg atacttgaga attcaaccgt ttgagatttt gcaatcaatt
    64501 tggtacgatt ttggacatcc atatttgcag tgaccgcagc aatatgtctt gcaccagggc
    64561 gaatgaaata attcacatac ctaatcagtg catctggctc cggtaccgtg tctgcatcat
    64621 tgcttaaaat caatttccct tttgcaaagg caagtccgat attaaacgcg tgggcttttc
    64681 ctttattttt ttcgatccga accacacgaa gattagcgta ttttttcatc agtctagcta
    64741 atatttctgg tgtttggtcc gttgaaccat cgtctgtcac taatacctca tagttgtgat
    64801 aattcaattt cgtcatcaaa tactcgattg tatcttcgat cacaatttct tcattatggg
    64861 ctggcaccat gatcgtgatg aacggttcga cactcaaggg aacatctacc cattcttttt
    64921 gcttatgttt gtataaaaac acataacacc agacaccaat aaaccataca aatccgccta
    64981 aaatcggata agaaacgaga aaaaatacat aatagacaaa caagtatcta agaataaatt
    65041 acctgtatgg gtgattgaga tcatttcagt tccaccccat tttctttata caaatctgca
    65101 atgaatgtcg tatctagatt tttctcttcg gagacagaat aaaaacggac cgtttctctc
    65161 tcttcttttg ttccaaaacg ttccgtatac acttcttcaa gtaattcttg tctttttttc
    65221 aatttttcat cttcatattc aaaagattta tttacttttt tgtatagatt tcgattgttc
    65281 cacctagtca atacaaatga ggtaatcagg aaactgaaaa aaatcaatac aaaaaagccg
    65341 attaagaaat acagcatttt cttttctgat aaaaaagcgt acaaggaaaa atccttggct
    65401 ctttctggtc tcgttaatgg gaaaaagacc catataaaag gaagaacaac gatcatccat
    65461 cctaaaagag ccactaacgt ttgccaaatc tttaaccagt aattaccttt tgaaaaatac
    65521 ttatctttca ccaaaattga tgcaacttct tctcttgtta cttttttcat atcctcacct
    65581 cacgtttttt tgttacgacg taggagttta ccatacattt catttctttt cacataaagt
    65641 gttcctaaat ttttcctaag aaaaagaaca aaacatttct catcagcaaa tgaaaaatat
    65701 tttgttcttt ttatcagttg ttctttttat tttttatctc ttaattatgt tgctctttcc
    65761 aatacgacaa tgcttttcat tcctctagat atcgccgtgt acatttgtct gcgtcttctt
    65821 tgctcatctc catcgttttc aaatggatat aagattacat gatcaaattc cagtcctttt
    65881 gctagactca aaggaatgat ttggcagttt tctaatccat atgaattttt tttaatcgat
    65941 tcttgtagcg tttgggtttc ctcttctgtt tgggtgataa ctgccgcttt ttctcctttt
    66001 agagaaacca aaatctcagc tagtattttc aacaattctt cagaggatcg aaaagagcgg
    66061 tattcgggtt cttttccttg cggtcggatc gatacgatgt cgatcttctc ttgatccaca
    66121 gcataggttt tgaaaagctc tgtgattgct ccgctagaac ggtaactatt tctaagatca
    66181 taccgatgga tcggcagatg gtagtcgtcg aaacaacgca taatatcagc aaaagtagtg
    66241 gaagaattaa agattgcctg attctcgtca cctactaaag taaagtgagt ttttggaaat
    66301 aattcgatca atagacctaa ttgtgccagg gtataatctt gtacttcatc aatcaataga
    66361 tagcgaaatg ctggtacatt taccttttca acaagtaaat gacgaatagc taatataatg
    66421 actgcttcat caacagtacg tgtgggttgc caagcataag ctgagccata tcgtttttca
    66481 taaattcttt caaacaactg atgctcatcc acccatgcca tttcttcgat ttgtctggtt
    66541 atttttcgat attttttttg taaaagtttc cttgcataag ctacaatact ttgttccgaa
    66601 tcatcagaaa tcaatttacc gaataattcc aactgcctat cttctgtcaa cgagagtacc
    66661 tgattctgca cagcaggact ttttgcttgt ttcaaaagat gattcttcca ttcgctcatc
    66721 agtgcctttt tcgttgcttg aagcctatcg atcaatttag gatgagcagg aactttctca
    66781 tatatttttt cgatcgtttt tttagaaagg actgtttttc catttttagt aagtccacgg
    66841 ataaaattcg gatgatctaa aaacaaagga tctgattgtt ttagattatc gataaacttt
    66901 ttgctacgca aacgttctgt ctgctccgat acattttctc ccgtgatacg tttgaaataa
    66961 gcctcttcac cttctatttc ctctgctgaa agctgtgaaa ccagctgtat gatggttaga
    67021 ttcagtggat ttctttcacc taatgatggt aggacattcg aaatatagtc gataaaccga
    67081 tgattaggtg atagaatcag caagtcatcg gatgtcatcg tatctcgata taaatagagc
    67141 agatacgcaa tacgctgcat gatagcagag gttttaccac ttcctgctac gccatttacc
    67201 aagatatttt gacttttcac atcccgaatg attgtgtttt gttccgattg gatcgtagca
    67261 gtgatagcct tcatttcatt ggtctcatct tggcctaaga cttctaataa tacgtcatct
    67321 tgaatagcta cagacgtatc aaaaaacttg accaaacgat ctctttccaa aagaaattgt
    67381 cgtctttctt taataaccgt atcgatttta ttttgacgaa ccgtataaga agatggacca
    67441 atcatgttat tgtaaaaaag ttcggcaatt ggtgaacgcc aatcataaac aagattttca
    67501 tctgcatcat ctgtgaaatt tgctgtaccg atataaaagg attcttttgg ctcattatcc
    67561 agaaagtcta caacgacttt tccaaagtag ggacttttca ataaacgttc gatctttgct
    67621 agctgatttt ctgcgttttt ctgtttgata ttcatctgat cgatttctct atttttcatt
    67681 tcaatcatcg aaaaagtatc caggttatct aagtaactgt caaaattcaa tcgaagctcg
    67741 ccagccattt ctttcaacga ctggtttcct tctatttttg ccgtatccat cgattcccgc
    67801 agttcgtttt ttttatcaat tagcttttga tacacgttct tcagatatgc ttcttctaac
    67861 tgtttttgtg tgttgaccat acacattctc ctcttttttt aaagtggtgg acccgattag
    67921 tttacctctt ttaagagtcg aatgcaagct ttttaacgag tatcatttgt taggatatat
    67981 cagttcttca ggtcggacag tttgatataa agaaaatgca ccatcaaggt tttttacgtg
    68041 gtagccagac tgcttcaaga ttctttccgc tagatagcta cgtaatccgc tatgacagct
    68101 gacgatatat tcttgttggc tgtctaattc atttaatcgg tcccgaagtt cattcaaagg
    68161 gatatgtttg gcttccttga atttgccctt ttccaattct tctgctgttc gcacatctaa
    68221 taaaattttt ccttttgcta gttctgtcgg caattcatgc cattgtacgg tttcactaag
    68281 cccttctgca atgttcattg ctgcatatcc tagcatgttt actggatctt tggctgagcc
    68341 aaatggcggt gcatacgtca attctaattc cggtaaatcg aagatcgtca aatgcccttt
    68401 gattgctgtt gctaaaatat cgatccgttt atccacacct tttgccccaa caccttgtgc
    68461 accataaatc tcgccagtct ctggatggaa gatcaacttc agtaaaatat ctgttgcttc
    68521 tggataatag cttgcatgat cttttcctga tacatgaatg acttcaaaag acagttcgtt
    68581 ttctttggct gttctttcac ttaaaccggt ggatgcggca gtcattccaa acgttcgtat
    68641 aattgctgtt ccaataccgc cttgatttct tcgagaaatt ccggaaatcg tgtccgcaac
    68701 ttgtctacct tgacgattgg ctggtgaagc aagagaaata agtgcatctt ttccagtgat
    68761 ttgctgtttt acaacgatag catcccccac cgcataaata tcaggaatat tcgtttgata
    68821 acgttcgtct actaggatac ccccgcgtat gcctaattcg attccagcgg tcactgctag
    68881 ctggttatca ggacgaacac caatggataa aataactaaa tcagcaggga tggtacgccc
    68941 atcatccagt cgcaatcgtt tcccgtggtc ttcaaatcca gcgacagcat gagaagtaat
    69001 cacttctact ccttgatgag acaattcttt ttctataaaa gctgccattt cttcatccag
    69061 tgttggaaga atatgtggtg cttgttcgat caccatgact tctaaacctc ttctttttag
    69121 gttttctgcc atttccaatc cgatgaatcc tgcaccaacg atcacggcac gctttggctg
    69181 tttttctaat gcatgcatca ctttatcgat atctggtaca tttctaatag aaaaaacatt
    69241 gtcggcttct gttatccctg gaatcgatgg aacaactggt tttgcaccag gagataaaac
    69301 caatgcatcg tactgttcaa tgtgtttttg accctcatgt acgacttcta ccgttttgtt
    69361 ttcgggaaag atggctgtca cttcatgatt tggacgcaca tctaaacaaa aacgagcgga
    69421 taatgattct ggcgtttgga caagaagatt ttcccgttca ctgatttctc ccgaaagata
    69481 ataaggcaag ccgcaatttg caaaagaaac atacggtcct ttttcaaaaa caacgatttc
    69541 tgcatcttcc attaatcggc gaagccgtgt agcagcagac attccacctg ctacacctcc
    69601 gacaataacg attttcatct tatttccctc ctactgttcg atcaaaccat tgattcatgc
    69661 caccacgtac attcactaca tcataaccag atttctttaa ttctttagct gcttgtttac
    69721 tcctcatccc tgattgacaa atcacataga cggtcttttc tttgtctcca tcatatctgt
    69781 ttatactttg taatggaaca tttttagctc cttttatatg cccgccccgg tattctgcag
    69841 gtgttctaac atcaagtaac gctggatttt ccaaaagctt ttcctttaat tctgggactg
    69901 ttatcgattt tattgattga aatagcaaat tcattttctg cacctcattt acatttttat
    69961 aaatacctca tggggtatat taaacaggag ctttattaat atgtcaacca gaatgactct
    70021 tcattttttt cactactttt tatatttgca taaaataccg ataaggggta ttatctaatt
    70081 ataaaaatag aggagaatgt gttatggaga ttgataataa aaatatcctg aaccgtttga
    70141 aacgtacaga gggacaaatt cgtggtatcc aaaaaatgat cgaagacgaa aaagaatgta
    70201 tggatgtcat cactcaatta agcgctgttc gttctagtat tgatcgcgta atgggcatga
    70261 tcgttgcaga taatctgaaa aattgctttg agaaacctga tagtaatcca gaagaacaag
    70321 cggctaaact agaacaagcg atcaagatga ttattaaaaa gtaaaggaat gatttcaatg
    70381 aaagtcgttt tccatattga tgaattagaa aaatggtcag aaacagggaa aaacgtaaaa
    70441 aacctgataa aagcttctcc agaaacgacg attgtcgtat ctgtaaatgg catcgcgatt
    70501 accggttatc tagactctgc caatgcagaa ttcttagatt tgcaaggagt cgtttttcac
    70561 gcttgcgcca atgctatgcg tgctaatcat atttcagaaa gttctttacc tgaacaagta
    70621 atcgttgtac cagcaggtgt ccttgattta gttgaactgc aatcccaagg ttatgcttat
    70681 atcaagcctt agtcagtaga ggttgcgaca gaagtgatca gcttcaagaa atttcggctt
    70741 atttttcgaa gaagctactt ctggaaaacc gtgaatcaaa ggttgtgaaa acgctgtcct
    70801 gcatcaagta ataagaacag ccaaacaaaa atagcttctc atatttttcg tttggttggg
    70861 cttattaccg cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaatcagcg
    70921 gtttgaccta agtattagag aaaaaaacgg gaaaatagct cctcatattt tgccgttttt
    70981 tgagctaata ccgaaggtca gctgattgaa caccgtttaa ggaagagatt gtgaaaaagt
    71041 cgcttagttt cgagtattaa gaaaaatttt gtgaaaatgg atttctacat ttttgcataa
    71101 atttggctta ataccgagaa actggctttt gaataccgtt taaggaagag gttgtaaaat
    71161 cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagctgctca tattttgccg
    71221 ttttttgagc taataccgaa ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc
    71281 ctaccattta actccgaatc acaagaaata atttcaaaaa caacttctcc tgtttttggt
    71341 ttggttggct tattatcgag aagtagctac tgggagtcta tttatcaaaa aaaggaatgc
    71401 gacataggtc acaccccttt tttattttct ttgaaacatc tttcggtact cgacagcata
    71461 caagacaaaa gattgtaaaa gaccgtaagt gatgacggca aaaatgactc tcacccacag
    71521 cgttggtaat tctgggaaaa gcagcaaaag gatcatgcca acatagatga cagcagtcgc
    71581 tactttgcca taccacttcg ctccacttaa tttgatattt ttagatagcc cgattaatcc
    71641 agctacaaac ataaagcctt cttttatcaa gaatagacca aacaacagcc aaagccacgg
    71701 tcgataccat gccatagaca aaatcagaaa aagttgtgtc aatttatctg caaacggatc
    71761 aagcaccttc cctaagtctg ttacctgatt atatctccga gcaatgaccc cgtctagata
    71821 atcagacaaa cctgagacaa caatgaccgc tcctgctata taaaatgact gtctcagcac
    71881 aaaataaaga taaatcggca gcaaaaaaat acggaatatc gataaaagat tcggaatcgt
    71941 ttgtatttct ttacgccagt ctgtgcttaa tttcatttta tcgccctacc tcctcagcac
    72001 tcttttcttt ttattttagc ttacacgggg aataaaacca atgatttcac tgtttttatt
    72061 tttctttaca gccactgata ttatgagcta atttgagata atcacttgct gttttcatcg
    72121 tttactcttc tttcttcctg tcgtttgcgc atttctttta ctagtacttt atgagctttt
    72181 tttgatcctc gctctaggtc acgctgccgt ttacgtgttt tataatcgat aaacagactg
    72241 tctaaatcgt aacctgtcgg gtacagatct tctgctgaac gcaccagacg aacacgctga
    72301 cgaggcacgt ggattatttt tttgtctaaa tatacatcaa tcgtatcttc acctatatct
    72361 tcataaacta acgcttcttt ttggtattca ttgacaaata tccgatcacc ttttgcaaag
    72421 agctggcgtt cttttttatt ctccttccta tttgtaactg ctgtcttagg aagaaattct
    72481 tttttctgtg tccgataggc tttttccgcc aagtaacgct ctgcttgatg gatcaatttc
    72541 atcgacatat ccattttatg agcaatccac aaagcttggc tttctcctgt ttctccaacc
    72601 cgcaactgat atttaggacg aagtgcttcc cgatcgaaag ccatagcagc cggcacaaaa
    72661 tcttcatgat ctccggcgaa tttcttgatt tctccataat gggtcgtggc aatgatcaat
    72721 gccccttgct catacataga ttccatgatt gcgatcgcta gtgcagcccc ttcatttggc
    72781 tctgttccac tgccaatctc atctaaaagt accaacgtat tcctttttat ctttttcaaa
    72841 atagcagcga tgttttgcat atgaccagaa aatgtactca acgcattttc taaattttgg
    72901 tggtcaccga catcgacgaa aatctcatca aagacagcaa gctctgtccc ttcttttgcc
    72961 ggaacttgca taccgaacat cgccatcaaa gtcaataaac cgactgtctt caacacgact
    73021 gttttgcctc cggcatttgc tccggtgata atcaagcctc gatagtcttt accgatttca
    73081 acatccaatg gtaccgcatg ctcaggtaaa aatggatgtc tgccttgctt gataatgata
    73141 tgttccgatt tattgattct aggtgtgctt ccattgattt ctcggctaaa ttttgcccga
    73201 gcaaagatga tatctaacac cgtgatcgtt tcgatcaata aatcgatcac ttcttcgttt
    73261 tctgcgatag caccagtcaa tgccgccagg acttgatatt cttcactgat ttcttcagct
    73321 tttaacaact gatagtgttc attcaattta gaaatcacag ttggttcaat aaaaaccgtc
    73381 gttcttttat ttgattcatc gataatcgtt ccatctattt tatttttata gctggctttg
    73441 ataggaatcg tatagtattc gcctttttga acaatcattg cttcttggat catttctttg
    73501 tttttcggat gacgcaagaa tttcagtaat tttgactgga tttctttttc gattagttgt
    73561 aattgtttgc gtactttacg caaattacgt gacgcgtcgt cactgacttt attatttttt
    73621 attttctggt caatcaactc ttctacattt atcaaatcag gaagatgttt gctataagca
    73681 aacaacaagg gtgtctggta ttgattctta tcaaaaaact ttgtgatcat ccgactgctt
    73741 ctaagaaaat ccgcatattc gatcaaatcg gttggctgca gaatcaaccc ttttttgact
    73801 tgttctgtca gtgcatcgat cctcggcaac cccataaatg gaacatgctg actgctttct
    73861 aagatcaagc gtgcttctct tgtttcttct tgacgatcca agacagtttg taggtttgtc
    73921 gagaccgtca tatcgctgat tcgttttttg ctatagtttc caattgctct tgcttggatt
    73981 tcctttttga tccgatcaaa ttgtaattga ctaatcattt cgttgtccat attctctcct
    74041 tttcttgaag agatacctgt cccaaaataa ataagggcct agagataaat aaaaggatag
    74101 aacacagctt aaaaaagtca tgtcctatcc ttattagaat acaaagatat tgttttcctg
    74161 acaggtaccg tttcataagc acaccaaata aactttgttt attggctatt cgacttactg
    74221 attagatacc agctacactc acaaaacata ccccattctt ctaggtctta tttattttcc
    74281 acttaactat agcaagacac attttggttg tcaaacttta gtatgtttta tagatttaca
    74341 ccattaatga atagacacct accgcgataa ttgctccgat cattggacct gcgattggta
    74401 cccatgaata tgcccaatct gaagttcctt tattagctat tggcaatacc gcatgtgcta
    74461 atcgaggacc aaagtcccga gcagggttga ttgcatatcc tgttggccct cctaatgaaa
    74521 gaccgatggc taagatcaat ccacctacag cgaatacgtt cgttccaggt gcaaattcgt
    74581 tttgtccaaa agctagcaac ccaagaacta atacaaatgt accaattaat tctgtgatca
    74641 catttgctgg ataattccgt atagcaggac ctgttgcaaa cgttcctaaa atcgcgcttt
    74701 catctttcgt tgcattccaa tgtggcaaat aagaaagcca aacaagaatg gcccctgcaa
    74761 aagctcctaa aacttgcgca atgatgaaag gaacgacaag attccaacta attgcccctg
    74821 tcatcgccat agctagagag actgcaggat tcaaatgagc tgggcccatg aatccagaaa
    74881 tatagacagc cattgttaca gcgagtcccc aaccaaatgc gatgacaatc cagccagaag
    74941 cctgtgcttt acttttgttt aaattcacag ccgcacacac accatctcca agcaaaacta
    75001 ggatcagtgt cccaataaac tctcccatga tttgagtcat atcactgttc atttatttct
    75061 ccccctttag ttcccgcaga tctgattctg caatcaatgc ttctaaacgt ttagtctgtt
    75121 gcgccttctc ctcttctgtc cattcaaaat atccagcaat tgcatcgatc acaggttgct
    75181 tgatctcatc aagctgatct cgttcaaaca acagatgatt tgtacgacga atgagataat
    75241 cgcctggggc caaaaccatt tcttcttcta aaccataacg taaccgagct gactcagcga
    75301 gactcaaacc aggataaggt gccatttctt ttgctaattc aaagatacgt cgggcattag
    75361 tcccgtaaaa atcagcgata taagtagcat cttcttcctc taaaccggct gctactcctt
    75421 cttttgctaa ttctgtcact gtttcttcta atttagttgg atcgaagttt ccacctgaaa
    75481 tctgatattt tttagagtcg atttctttcg tctctattcc gtattcttct tttaataatt
    75541 ggcgtatcaa tcgcaaagct ccttctgcca tcttacggta atcggtgatt ttcccaccag
    75601 ataaagtcag caaaccatct ggttctcttt ctaaagaact acctctggaa atcgtagaag
    75661 gtgctttttc atcacggctg ttttccaaat ggttcaacac gtcttctact tcagcacgag
    75721 aaactttgtt ttccttatat tcacttactg tatcgacaac tttattgaag ctcttgtctg
    75781 aaatcgatcc attatctcca ccattgtaat cagaaccaga attaccaatc aacagtggac
    75841 gaagtcctgc ccagcttgct tcgatatcat tcaatgtgat atttgcttct ggatagcgat
    75901 gattgatcac atccaataga taatctacat cttcttgtgt gactttcgga tccgtaaaat
    75961 ctccttggta atccgtatct gtcgtaccaa agtaagtctt gttttctctt ggaatagcaa
    76021 aaaccatccg cccatcttgc tttcctgtat cgaagtatgt gggttgcggt acaggcagtt
    76081 ttttcgcatc tacaactaaa tggatccctt tagttggacg cattttagga gagactggac
    76141 gcgtaaaatt caagtttcgt actttatcta cccaaggacc actcgtattg atcactaatt
    76201 ttgctttgat ttcgatcact tcatctgtca gcaggtcacg agctttgacg ccaacgattt
    76261 gatcgccatc atataaaaag ccaaccgctt tcattttact tactagataa gccccatctt
    76321 cggcagcctt tttgatatta tcaatcacta aacgggcatc attgttgcgg aaatctagat
    76381 agacacccgc gccttttagc ccttcttttt tcaaaaacgg ttctctttcc aatacttctt
    76441 ctggtgtcag cgtataattc tcatatttgg ttcctgtcac atttgccagt ttgtcataaa
    76501 ggtccattgc tactttgacc gagaacatat tgaaggttgt tgctccttcg ccctcataga
    76561 ttggcaaaag cattggatct ggttttggga tgtgtggggc aatactttgt acgactgcac
    76621 gttcaccaac tgtgtcagct actacttcca catcaaatgt cttcagataa cgaataccgc
    76681 catgaacaag tttggtcgag cgagaggaag tcccttctgc aaaatcctgc atttcgatca
    76741 atcctgtttt gattcctgat gctgctgact ggactgccac accggcacca gtgattccgc
    76801 caccgatgat caacagatcc aattcttctt gctgcatttt ttgaatgcta tcttgtcttg
    76861 tcttgtttga aaacatatgg atttcctcct tattcaaatg cttgggtagc ttttactgct
    76921 tttttccaac cgttgtaaag tttttctcgg cgctcttcct ccatttgaac ttcaaatagc
    76981 ttgccagctt cataaaattc gcggatctcg tctgtatctt tccaataacc gacagctaat
    77041 cctgctaaaa atgctgcccc tagcgccgtt gtttccaagt tttctgctcg ttggatcggt
    77101 acattcaaga tatctgtttg gaattgcatc agatattcat tgtttgctgc accgccatct
    77161 actttcaata caggaatctt gattccagtg tcatcttgca tcgtatcgat gatatctctc
    77221 acttgataag caatcgactg taaagtagct ttaatgatat cttcttttgt cgtaccacgt
    77281 gtcaagccaa acatagcccc tcgtgctgcc tgatcccaat aaggagcacc tagcccaacg
    77341 aatgcaggaa caacataaac ctcatcttcg cttgtagagc gtttcgctgc gtcttctgaa
    77401 tcgctagctt tttgcaacat ttgaagtcca tctctcagcc attgaatcga agaaccagcc
    77461 acaaaaatac ttccttctaa agcatagtaa acctttccat ttatgccgta accaattgtc
    77521 gtcaataaat tatttttgga taattgcggt ttttcaccgg tattcatgac aataaatgaa
    77581 cctgtaccat aagtattctt gaccatgcct ggttcgaatg ccatttgtcc gaataatgcg
    77641 gcttgctggt ctcctgccat ccctgaaatt ggtgcttcgc ttccataaaa atgataacct
    77701 tgcgtataac cataaacttc tgagttagaa gtgacttttg gtaacatcac tcgaggaata
    77761 tttagtaaat ctaaaatctc ctgatcccaa tctagctgat ggatattgaa caacatcgtc
    77821 cgactggcat tcgagtaatc agtaacatgt gcttgtccat cggtcagttt ccatactaac
    77881 caagaatcga tcgttccaaa catcaattcg cctttttcag ctcgttcttg cgcgccttct
    77941 acatgatcaa gaatccagcg tatcttcgtt gctgagaaat aggcatcgat cactaaccct
    78001 gttttttcgt ggaaaaattc ttgatagcct tcatttttta agttatctgc aattccagct
    78061 gactgtctgg attgccaaac aactgcatga tagattggac gtcctgtatc tttttcccat
    78121 attactgttg tttcacgctg gttcgttatg ccaatcccag caatctgatt tggtttgatc
    78181 cctgattcga tgaacgctcc ggcaataact gactgtacag aattccagat ttcatttgca
    78241 tcatgttcca cccaactttc ttgcgggaaa tattgcgtga actctttttg agaactgccg
    78301 atgtgtcttc ctttcttgtc aaagataatt gctcgtgagc tagttgtacc ttggtcaatc
    78361 gccatgatat acgttgattc tgtcattgtg attccctcca ttatatgcaa acgctttctt
    78421 taactactct tatcatacta ctgttttaaa aaaagaaact gtcaaaattt tattcttttt
    78481 ttcttttttt gacagttttt tattatgaag ctatttttta atcaattctt ttcttttttc
    78541 agcgtctcta ttcttctttt caatcgaata atgtcgtaag gtgacgcaaa ttctgacaga
    78601 taatattcac actgtttttt gtttctctgg caaaatgtga tcaccaaatc atattcttta
    78661 ccttcttcat aacttccaat ctcaataccg ccgattcccc tcagttctct gatcagatat
    78721 tgttgaaaag caatacgata aatcggcaga ctttctaaat cgattccaat cactactggt
    78781 tttgcaatat aaaaatcgat catcaaaaga acattggcat actggatcat caggtaacta
    78841 agctcactat cttcagctct tttcctcggc aattgctgca ccgctgtttc ctgtaatttc
    78901 gccagtaact gtgttaagga atgcccgagc attttctgct gctgttccaa aagccgatca
    78961 cgctcataga cttcgatctt tccaacaaag aaaaaccatt cattgttgat ctgggaaatt
    79021 tggtagccta ctgccttttc ttctttgatt ttcaaacgac gaggacgata atgccataac
    79081 atcgtttccc gaatgtaagt atctaaaaca gcggttggta actttttgct ccgtgtcaaa
    79141 tcatactgat aaaatatttc ttcgtctact atcgaaaaag aaacaaaaaa gctgtaaaac
    79201 atcattgttt cgtatacatt cagttctgct gctgttcgac taagatgcat cacgatgatt
    79261 ggatcgattg cttgataaag ccggtccgac tgatagagta atttcttttc tttcaaagtt
    79321 gcgtacgtac ttttttcatt caatagtctt tttttcgtaa tcccgagcca gcaagcaatc
    79381 tgtgcttctg ctgatgccgt aaacgttgtt tctaatacac ggtccaatcc tagaatgaac
    79441 ggtctttttt ctggtgtatt ttgcaaaaaa agcggtcgct gatcttccgg gatatattgg
    79501 aatagttcat aataaaaata tcgtatctgt agctcttctc cgtataattg accgtttttg
    79561 atttgtaatc caaattcctc taagagctgg ttcaattctt tgatctttcg aaaaacagaa
    79621 gactcactga tattaaacgc tgtcgctaat cgaacgatgg atacttgctg atgttctaat
    79681 aaatagacga gcatcttaaa cttcaagctt tcctgcaata aaaacaatag aattttttcc
    79741 aaacttacat cttcgtccaa cacaagcagc gcctgatact catttcgctg tacttctact
    79801 tttttttcca tcattcggcc aatcaattca atatcttcca aatattgatc aaacgaggct
    79861 tttgacagtt ggacatgctc tctcaaacca ttgatagagg ctgttccccc ttgcagaaca
    79921 aggtttcgca aaatcaggat ctgtaactgc tcttttcttt ctaataatgc ttcaatcttc
    79981 ataggcagta ttcgccacct ttgaccacac catatctggc acacggtatt ctggctggaa
    80041 aaatagctca tgttcttcta actgttcaaa cgtccatcct tcttctactg caattgtcag
    80101 ttggtcaaga aggtggaaca tctctttttt gctttggagc tgtgctccaa gaatcttttt
    80161 ttcagaatca aatagaaatt tgagtttaac tttgctttga tttattgctg taagcttgta
    80221 gtctacgtat cctgtttcaa ttttttgggg ataaaaaatg ctctcttctt ctgtcaaacc
    80281 tacgcttcct aagaaatagc caaaccactg attgccgact ggacgatagg ttccctgatc
    80341 ctttgtctgt gagccactaa ttgtcttgct gacatttcgt gctgtacgga cagcgttatt
    80401 tactagagaa acatacattt tttcatttgt tggacgaaaa gttacttgga tcgcatcgcc
    80461 aatcgcataa acgtcttttt gagacgtttg caagtagtca tctacccaga tcgttccatc
    80521 atcattcaat gttaattgtt cttcccacat ctgattgtcg gggcgagaat gattagccag
    80581 caaaacaaga tcatagttct cctttctttt ttccgtctct acaatagcgg tttcattttc
    80641 ggtcaaagac acaacttgtt cttctaaaaa aagtgaaagc ccttgatttt tcatttcctt
    80701 tactaacggt tctgtcattt caggatctag ataacgaaaa agaattgact ttcggctttc
    80761 gtaaagatgg atttgttttt ttcttgatgc caacccttca gctatttcga ttcctgcttg
    80821 tcctgcacca ataattgcaa ttttctttgc attcatgatc ttttgctgaa gagccgttac
    80881 gttttgtatc tttttaactg ggtgaataag cgttgattct gcagaaatat ttcgaaactt
    80941 ttgatttgaa ccagtcgcta gaatcaaacg atcaaaatcc aatttttttc ctcctgctaa
    81001 aacgactttt ttttctttca atcctattac tgtcttttct gtgtaaacat ggattcgata
    81061 gcggtcagtc agttcttctt tcgtgatcca agaaagttgt ttttcactgg taacctttcc
    81121 ttttaatatc aaattgaacc cgctgggaat ccatcctaac ttcgattgct tttcaatcaa
    81181 atatatttca cttgttggca tcaattggcg aagataaata gccgcatgaa tccctgcaaa
    81241 cgacccgccg acaattacaa tgttcatctg cattcctctt tcttataagg tgttctactg
    81301 ctaacttctc aatcagaaaa cacagtattc ataactcgct ttcaacctta tgatacatag
    81361 cgatgcagca gaatcaaccc tctccatcat accgtgttct aggtctgctt ctcaataatg
    81421 tactagtgga cctaattgaa gagtatcatt tctgaagaga tgtttcaaaa aatgtactct
    81481 tataaaagaa aaaaactcgc cttttacgta cctctgtcca aataggaaac agaaaagaac
    81541 gaaaagacga gttttagcgt gtgtaatgta atagttagaa tagacacatc atttattttt
    81601 aattctttaa tgcttcaaca actgcttcgc ctaattgatg agcagattga ggattttgcc
    81661 ctgtaagaag ccgcccatca atttgaacca cttctgaaaa agctgctcca gactgatagt
    81721 ttgctccttt ttctttcaaa gcatcttctg ctaagaatgg tacttcattc gttgtaccat
    81781 ttgcttcctc ttcttcatta ctgaacccag tcactgtacg tccagctaaa atagattttc
    81841 cagactcatc tgtaactggc aacaatccta caacaccgtg acaaacagct gagaccagcc
    81901 cttcatttcg atagatttgt gaagcgactt tcccaagatc tattgcttct ggaaaatccc
    81961 acattgttcc gtggccacca gcataataaa ccgctgcata atcatttgca tccacatcat
    82021 caggacgtaa ggtatttccc aaggcccgat ttctgaaatc ttcatcttca taataggccc
    82081 aatcgatttc atccatattc aaaacacttt gcggttctaa aggtacatat ccgcctttag
    82141 gactgacaaa atctgcatcg atcccatttt ttactaatac atcataaaaa tgagtcagtt
    82201 cacttaacca caaacctgta ggacgctcga tcttttcgta cttttctaca tttgttaata
    82261 cgatcaatac tttcattttc ccatcctctt tctataaaaa ttgcgttatt gataacgtgc
    82321 tttcaagaaa ttatataatg ggcttggcaa ataaagctct ttatatgctt cttcaaaagg
    82381 tttcgttaca aattcatcct cacggttttc caataatttc tctgcccagt caggatcgac
    82441 gatcatttgc tgtccaattg ctgcaagttc tgcatatttc aatacctgct ctacatcttc
    82501 gcgattgcgg atcccaccga caccaactaa agggatcttt ccttgaatcg tgttatggac
    82561 cacttcaaga atcgatttat cgttgtattt atcagaacga gctattcgat catacatatt
    82621 taatgaaaca tgcagataat ctagttttga ctcacgtaat cgttcaagaa gccagatcgt
    82681 gtcttcaaaa cggatacctg gtgtttcata ttcttctggt gagaaacgat accctacgat
    82741 gaatggtttt ttagcatatt gttcaaccgc tgagaaaacc gttgttaata attcttcaat
    82801 aaaacgataa cgattttcca agctaccgcc ccattgatcc tctcgtctat tcgaatgcgg
    82861 agagaaaaat tgttggatca aatacgtatt tgctccatgc aactcgattc catcaaatcc
    82921 tgattcaatc gctctacgtg tggctttgcc aaatgcttgg attgtttcaa cgatttcttc
    82981 ttcactcatt gctcgtggtg tttccgcatc aggacgtttt gctgcaactg cacttgcaga
    83041 tacaggctgt tctccagata aagttgctcg acttgtcatt cgtccaccgt ggaaaatctg
    83101 aataatcgct tttgcgcctt ctccttggat tgctttcgct agctcactta atcgaggaat
    83161 catttcatca tgagcgatgc tgagttcacc tggccagcct tttccgatat cttggacatt
    83221 tgcagcacca gtaataacag caccaagacc tttagaacgt tgtttataat aagtgatctc
    83281 atcttcggtg atcactccat tgaaaaagct gagttgtgtc gtcataggcg acagcatcaa
    83341 tcggttcttc aattgaactc ctgaaggtaa agtaattgat tgatcaaatt gtgacattgt
    83401 cattcctccc ataattcgct ctgattaacc tatcgagaat catctatcat taaaccactc
    83461 gattagttgt acatgcaact aatttgctta aaaaatttta ttttttttgt aacttatctg
    83521 ttgcatcatt cattgtctgg gctaattctt tacttgcttc atttcccaaa actgctttgt
    83581 atgactgatg aagttcttcc cagctaccat cgacttcctt agcaaattgg cgtccttgtt
    83641 ctgtaagata aactaatgac aagcgacctt cgctaactgt tttgacgtat cctttatatt
    83701 ctaatttttc aacaaaacga gcaatcgttg atggggcgga atagagcatt tgtgcaatat
    83761 ctttttgtgg tattccatct ttttctttta atatcaataa aataaaacca taagttggtg
    83821 ccagacctgt ccttctaaac gcttcatcag ctaatttatt caacatgcga tctagctttt
    83881 tcacgctaaa gaaaagacat tcatctaacg ctttttcaat tggcttttcc atttcatccc
    83941 ctccattggc gtacatatca tagtatgatt tttatttttt gtcaaaaaac acggaataat
    84001 gtccgcctcc tctgtctaga tttgaaaaaa agaagtagcc atcttcaaag aatttcggct
    84061 tgttttcgac aaaactcctt ctggaacacc gtgaatcaga ggttgtgaaa aagtcgttta
    84121 gttttgagta tgaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttgg
    84181 cttaataccg aaaaactggc ttttgaacac cgtttatatc agaggttgtg aaaaagccgt
    84241 cctgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatatttt gtcgtttttt
    84301 gagctaatgc cgatggtcag ctgattgtac accgtttatt gagaggtcgt gagcctgccg
    84361 ttgaactccg agtcacaagg agcaatttca aaaaacagct tctcatgttt tatgactatg
    84421 gtgacttgtg atcgaggagt tggcaggaga atactgttta ttcgtaaaga aggggctgtg
    84481 acaagacttt tgtcacagtc ccttatttcc ttttattata ggatttcgcc atttgattgg
    84541 atgacttgtc tgtaccaatc aaatgatttt ttctttaacc gtttctttgt gccattccct
    84601 tcgtcatctt gatcaacata gatatatcca taacgttttg acatttgtga agtagaagcg
    84661 ctgacgatat caatgcagcc ccacgacgta taaccaaata aatctactcc atctttgata
    84721 gcttccttca tctgctcaat atgctgtttc aaatagtcaa tgcggtaatc gtcttgaacg
    84781 gttccatctt cttctacttt gtctatagct ccgagtccgt tttcagctat gaacagtggt
    84841 ttttgatacc tatcatataa ttgattcaaa gcaattctca aacccttagg atcaatctgc
    84901 cagccccatt cacttgatgg aagatacgga tttttaattc ctccagttat taagtttcct
    84961 tggacccctt cccaattttc tggatgagca ctgactgcac tagacatgta gtagctaaaa
    85021 gaaacaaaat caacagggta ggctgctaag atttcttcat cacctacttc tatttttaag
    85081 ttgacacctt tttcttcaaa cattcgttgc gtatacgctg gataatatcc tcttgcttga
    85141 acatcactaa agaacaaaga agttctttgc tgctcaattg ttttttgaat atcatccgga
    85201 tcacaagaat acggaacaag aagctgatag ctgatcatac aaccgacttg tgaacctttg
    85261 ataatttcat gacaagcttt tgtagctaaa gcacttgcta taaactgatg gtgtgcacct
    85321 tggtatttcg cttgttccaa atggggatgg ttttcttcaa ttactcctac gctaacaaaa
    85381 gggtgatgtt ttacacagtt gatctcattg aaagtcatcc aatattttac tttattttta
    85441 tagcgtttga atactgtttc agcgaaacga acgtaaaact gaataagctt tcgattaggc
    85501 catccaccat attctgtaac caaatataac ggcatttcat aatgggaaag agaaacgact
    85561 ggctcaatcc catattttag acattcgtca aacacttgat cataaaaaaa taatcccgct
    85621 tcattgggtg tttcttcatc accatgagga aaaattcttg tccatgcgat agacatccga
    85681 tagcatttaa aacccatttc agcaaataaa cggatgtctt ctttaaagtg atgatagaaa
    85741 tcatttccgt gtcttttagg ataataatac ttgctgttgg gatcctgcgc cgcttttact
    85801 tgctgatgcg tcatcatatg ccattgatct gtccattttt ctttaggtaa atcgctgtcg
    85861 aatgtataca catcagacac agataatcct ttttcatcta catcccatgc accttcacat
    85921 tgattggccg ctgtcgctcc gccccataaa aatccttctg gaaatttttt catatcgatc
    85981 cccctaaact tgctagtttt cttgtttgat tatttttctt atgactagta atgatatttt
    86041 tgaatctact actatttgta acaataacca ttgtggtcgg atcatatccc tctttgatta
    86101 attcttgcaa atctacttcc atcaataaat cgcctttacg tacttttttt ccatcagata
    86161 ctgcactttg aaagtatttg ccatttaaat tcactgtatc aataccgata tggatcagta
    86221 tctctgctcc ttcttcactg actaccccaa ttgcatgttt cgttggaaaa actgttgaaa
    86281 cttccccgtt tacaggactg tagatttttc cgtccgttgg aatgatcgca aacccttctc
    86341 ctaaagatcc actagcaaat gttggatcgt ttacttcaga taaatcaatt atttcacctg
    86401 ctgcaggagt acttaacact atatcctttg aggattcttc ttcgatttca tccttgtagc
    86461 cgaaaagaaa agttagcata caggaactga tcatcgctaa gaaataacta gcaactagcc
    86521 aaacaaatcc ttcaccaata taaatcggga aagacaaaat acttcttgta gcaactgcca
    86581 aagcaccggc tcctgcggca ccagctactc caccaaatat agctccacat atacaagcaa
    86641 tgtaaaatgg tcgtttatat ttaagagtca caccataaac agcaggttca gtgattccaa
    86701 ataatgctgt gataaatgca gataaagata aactttttac tttcttattt ttcgttttca
    86761 aaaatacccc aagtactgct cctgcttgcg cagctacggc agtcatacat gctggaccta
    86821 gcgtatcacg accgtagaaa gaaagattgt tggtcattac tggtacaagt ccccaatgta
    86881 acccaaaaat cactaacact tgccaaaatc cgccgataac agcaccagat aaaacagggc
    86941 taaagttata aacagctaaa tatccagctg caatcagctg acttgcataa tctgcaattg
    87001 gacctacaac taataaagta attgggacca tcacgatgat acaaatgaat ggtgtcaata
    87061 aatttcgaat actttcgtgc aagcttttgc gaatcttagg ctcaatataa gaaagaaccc
    87121 atacagctaa aattgctgga ataaccgtac ttgtatacct tgctagaatc acgggcatcc
    87181 ctagaaatcg caaggttatg ctatcactat atgcactgac aatcgtagga taaacgagag
    87241 ctgctgcaat aaccatagcg atgaaacgat ctgtattgaa cttttttgca gcagtatatg
    87301 ccaaaaatat aggcaagaaa tagaaaaatg catcagctgc tgcattcaaa atgatataag
    87361 ttcctgattc agtagttaat aaattcaaac tcgtacataa ggcgagtact cctttgatca
    87421 aaccacttcc tgccaagacg cctaaagtcg gggcaaaaac agcggcgatc atgtctataa
    87481 aagagtttaa aatgcctttt ttctcagttc ctctctttcc actagatgca tcatcttcta
    87541 gtttcatttg tttggataat acgtcataca cttcatttac tgcatttcct actacgactt
    87601 gaaacattcc tccagcttcg actacagtga tcacaccttc tagttgctct accttctcct
    87661 tatctgcctt cttattatct tttagggtaa agcgtaacct tgttgcacaa tggtaaacat
    87721 tgctgacatt ttcttcgcct ccgacaaatc ggacaatatc tttcgctaat tgtgaatagt
    87781 ccatttttac ttcccccttg ttaattaatg ccgtagcaat ccttcttaca agataagtat
    87841 agttcttaga aaacggtttc tccattcaat tagtacccct tataatagcg aacgaaactg
    87901 tacaatagtg tgtatggttt acttagccga gcaaatcact cactgcttga aagaaaacaa
    87961 gtatatcgtt tctcttatag tcaaaggaga caaaaaaaac acgcttgatc ttcctttgct
    88021 gatgtataag tggtatctca agctgatact ctgtgatttt tgtttctact tggcttttga
    88081 taagacagca caacaaagtc tcggcattta acggaagcat aactaggtca gaactaggag
    88141 agattagttg ctgaatggaa tctatctttt tatggatttt tatttgattt tcttctttgt
    88201 agtaatgatc aagcagtttt gttttctttt tttcttcaac tatggtcagc ttttcacgca
    88261 aaatctgata aatctcccaa gattcaaact catctagaac actaggtaga taaatcaaat
    88321 catcccactc aaaaataagc tcttgctcag tagtcaaaat tgcatcatat tccgttattt
    88381 tgtttgattg atttttgaaa agatacactg gttctatccg gcattctttg atccacatgt
    88441 tcatactttg ttgaatctgt ttttttagtg tattcaaaat actaattggc tggttactaa
    88501 caatcagtat ctccccttgt tttcgaaaag gattcaacgc ttcggcaaga tacaaaacaa
    88561 aataagcgct ttcagctaaa ttgggacgta attgaaaatg tattgggaag aaacgccgaa
    88621 ttaaataaat ttctaaagga taacgggaga tgatcttttg tacaaaatga ttcatggtat
    88681 tatgtccggc atccatcctc gttttcattt gtttgatatg ggttagcaaa aattctgttt
    88741 catggaacaa taacgaaaca ggaagatcta aaaaacgaat aatcgtttgc tcaaatgctc
    88801 ttagattctt ctctatttct ttgttttttt catcattaat catcaaataa tttaactcta
    88861 atagtcgatt ttcaattgct tgtttttctt gattggaaaa agaatagggt agttctgatg
    88921 ataacttttt tgtgagggtc tctaccatcg gaataaataa tgtatacctg tttgtttttt
    88981 ctaaaagttc ttctcttcga agaatggtca aaacgagata ccgagaaaat cgtatgaagt
    89041 cctctcctga aacaatataa ttatatgata taagaacctc ttttaatgtc ttttggacag
    89101 tcggcatgat attggaaaaa attgtactga tttgttgagg caaccttgcg tgttggatca
    89161 ccgtttctgt tccaacaaga gataagaaaa ttctttttga attttcttca gcaatgattt
    89221 ttagtccttt tgtcgacgaa acttccagtt ctaatttagg attgcgctcg atccaacgca
    89281 aaatcgtttt tatttgtaat gatacggctg tttttgaaag aaagaaaatg tctgctatct
    89341 tttgcatact gacataccct ttttgaaaca ataagtacgt cacaatagtt gaagctcgga
    89401 aatccatgct ggaatagatt ttatgtttcg tcacctcttg tatgatttgc tgtttagttt
    89461 cagattcaat tgattctaaa aaataacctg aacgtttatt tgactggata atgattttct
    89521 tctcgtagat agcaagcata tttttgattt cagtttggat cgttttcttg ttggtaccta
    89581 aagcttcagc taattcttcg cttgttatcc aatgattctt atttcttaat actacaagaa
    89641 tttggagttg tcgtttattt aagtccattt gcttacacct cctctttgtt ataaacatac
    89701 aggaaaaacc tctcaaatga caatagtcac ttgcagaggt ttaaaaatca tttgaagaaa
    89761 gatctatatt caaggttttt accattagac tggataattt gtttataata ttcaaaacta
    89821 tcttttttca atctcctaaa gcttccgctc ccatcatttt gtctatccac ataaataaag
    89881 ccatatcgct tcctcatttc ccctgttcct gccgatacga tatcgattgg tccccaccat
    89941 aagtaaccaa tgatttggca accatctttt attgcctcag caatttcctg aagatgtatt
    90001 tttagatact cacaacgttt tggatcgtgg attcgattca attcatctgg tgcttcatct
    90061 agtccaatgc cattttcaac tatgaataat ggtaattcat aacggtctgt caattcattc
    90121 atgacataac gaaaccctag aggatcaatt ggccaactcc aaggttcggg agatttttcc
    90181 ttcaaaaagg gattatcgaa accatttgct ccgccagtat ctacgcgcat ttctgcttct
    90241 tttttataga cagcgctacg ataataacta aatgcaatat aatctgccgt gttagcttgc
    90301 aagcactgtt cgtctccatc taacattact ggtgcacagt cttcttcctt ccatgtcctg
    90361 taaatataag ctggataatg ccctcggcac atgacatcgg agaaaaacca tgaattgcgt
    90421 ttaaattgca gtgaaccaaa tacattctct ggatcacagt catatgcata agtcgctaat
    90481 gcagaaaagc tacacataat acccatctta gcatttggat cgattttctt taataattga
    90541 gtagcacggg cgttcgctag aaacatatga tgacaggctt gataaatcac tttttttgtt
    90601 agtgtagcaa tgggttcaag tttatctcta gcagggaatg taaccaatcc agcagctgca
    90661 taaggcatac gaaaagcatt attgatctca ttaaaggtca tccaatattt cactttccct
    90721 tggtagcgtt caaagacggt ctgcgcataa aattcaaaaa agtcgattaa ttttcgattt
    90781 tgccaccccc catactctcc taccaatgct aagggggttt cataatgact aagagtaatt
    90841 actggctcga tatcatatcg atttaatgta ttgattagtc tgtcataaaa tacgagacca
    90901 gcctcatttg gcttctgttc gtctcctctt ggaaatattc gtgcccatga gatgcttgtt
    90961 cgaaaagctt ttaaacctaa ctccttcagt aaacgaatat cctcttcaaa tcgatgataa
    91021 aaatcaatcg cttcatgaga aagataatgg agtgattcgt ctaaagcaat ctcccaaatc
    91081 tttttttctt cattccactg aatactagga tgctggttca tgatcccgat aagcgtatct
    91141 gtcacattag gtaatttgcc gtcttccaac catgcacctt cacattgatt agcagctact
    91201 gctccgcccc ataaaaaatt ttctggaaat atttcctgat ccataactat ctctcctcgc
    91261 tatcaacaat ttcttacttg aagtatagct cgattcatca aaacaagata ttctaactat
    91321 ttcggcagaa gagaagaaaa aaattgaatg ctactcttca tttttaatat ttatatataa
    91381 caaatacata agacgatccc cgaaagaaaa cactcgttaa ccatctaagc aaacgattta
    91441 ttttacttcc aacacaaatg cttcataagg tcgtaaatca actttgccgt ctggtaactg
    91501 tttcagagga taatttgaca caataaccct taaagcttca cgtggaacag tgaacgtctg
    91561 cttttcactg gacatattac aaataacgag ccattgctgg tcttcatata tgcgagaata
    91621 ggcaaagaca tgctcaggcg tcttttccac taattcatag tctccccaga cgatgatgtc
    91681 atgttttcta cgcaaatcga tcaatttctt gtacatataa aaaacagaat cttgatctgc
    91741 taacgcttct tctgcattga tttctttata atttggattg actcgaagcc atggaattcc
    91801 agtagtaaac ccagcatgtt cacgggcgtt ccattgcata ggcgtcctag cattgtctct
    91861 tcctttcgca ttgattgatt ccaagatttc ctcttttgta aatccagaag aaatcctttc
    91921 gttatacata ttgattgtct caatatcttt tgcttccgcg atatcatcga ttgggcaatt
    91981 cgtcatgcct aactcttctc cttgatagat ataaggggtg cctttcatca tatggagcaa
    92041 gatagcaaac atcttggcac tttcttttcg atacacatcg tcatttcccc aacgagaaac
    92101 aattcgcgga agatcgtgat tattccaaaa taaactattc catccttcat cacctaaaga
    92161 tgtttgccat ttggataata cttttttcag ttcagagata ctcaagggtt tcaaatccca
    92221 tttgtctttg ccttcttgct gatctaatcc tatgtgttcg aattggaaga ccatggataa
    92281 ttcatttctt ttaggattgg aataaagttt agcgatttcg gtagttgctc cccatgtttc
    92341 gccaacagtc atcagatttt tgtcaccaaa agttgcttga ttcatttcct gaagatactc
    92401 atgcagttta ggtccattac ttgtgatttc tttatccggt tctttgccaa tcatatcgat
    92461 cacgtccata cgaaagcccc caataccttt atcgatccag aaattcatca tttgataaat
    92521 ctcttggcga acctgtttat ttttccaatt caaatcaggt tgacgcttgc tatacaagtg
    92581 cagaaaatat tgccccgttt tttcatccaa ctgccaagca gaaccactga atattgagcg
    92641 caatctatta ggctcttttc cattgaccgg atcacgccaa atataatagt cccgataagg
    92701 attttcttta tttttacggg cctcaatgaa ccacggatgt tcatcagaag tatgattcac
    92761 caccagatcc atgatgatac ggatatttct tttctttgct tcttcaatca gttcttccat
    92821 ttcttccatc gtaccgaatt ctgccatgat tgcttgataa ttactgatat catacccatt
    92881 gtcatcattc ggagactcgt atactggaga aagccaaatt gcatcaatac ctaaatacgc
    92941 cagataatcc aacctggaaa taatcccccg caaatctcca ataccatctt gatttgaatc
    93001 ttgaaaactt cttggataga tttgataaac aactgaatgg tgccaccatt tttgttccat
    93061 gattttcctc ctataaatgg atgtttctat tttttgctta atcatttatt ttagtctctc
    93121 tcactaaaaa ttgtaacgct ttcaaatagc tattgcacct tgttatcggt aacagaataa
    93181 aacaaaaaaa cagctgagga aattccctca actgtttttt gaaaggattg atttatgcag
    93241 ggaagaatgc tgatttcaca acttctttat aaacggtgtt cactcagctg tcccttggta
    93301 ttagctcaaa aaacggcaaa atatgaggaa ctattttttc gtttttttct ctaatactca
    93361 ggccagaaca ctgatttcac aacctcttta taaacggtgt tcactcagct gtccctcggt
    93421 attagctcaa aaaacggcaa aatataagaa actatttttt cgtttttttc tctaatactc
    93481 aggccagaac gctgatttca caacctcttt atttcacatt caatatccac atgaatccca
    93541 caaatacaaa gaacagacca tacatgattg gagatacttc ttttcctctt ttagctgcta
    93601 acatggtaat tggatagaag ataaaaccta atgcaatccc atctgaaata ctataagtca
    93661 aaggcatgcc gatcaagatc aagaatgaag ggatcgcaat ctctaagttt ccccattcga
    93721 tttgtcgcaa agattgtgcc atcaatactc ctaccataat caatgctggt gcagttactt
    93781 gtgaagtaac aactgatagt aatggagaaa agaacaagcc taacaagaag aaaatccctg
    93841 tagtaatagc tgtgatacct gaacgtcctc caacggcgat accagctgat gattctacaa
    93901 atgcaccaac aggagaagta ccaaataagg aacccgcaag catagctgtt gaatcagaag
    93961 ctaaagcttt ccctacacga ggcatcttgt tgtctttcat gaaacctgct tggtttgcta
    94021 atccaactaa agttcctgct gtatcaaaga aagtcacaag caagaatgtc agcactacga
    94081 cccacatttg aagagaattg atgtccccta catgttttaa tgcaactaaa aatgttggtt
    94141 tcaaacttgg tgctgcggca acaatttcag aaggcattgg aatcaaacct gtgaccagac
    94201 ctaaaatcgt tgtagctgct atcccgatga agatgccacc tggtacacgc cgaacaagca
    94261 agatagcagt tacgactaat ccaaaaatcg tcaaccaagt agaaccaacg ttgagtgatc
    94321 ctaatcctac taatgtagat tcattcgaaa cgatgatccc accttcgctc agaccaagga
    94381 aagcgataaa tagaccgata ccgccagaaa tcgcatattt caaatctgct gggatcgcat
    94441 caatgatcat ttcccgcagc ttgaagattg tgatcaaaat aaaaatcaaa gaagcaacga
    94501 atacgccggc taatgctgtt tcccatggaa tccccattcc taaacaaaca gaataagcaa
    94561 aaaaagcatt gatcccaaga gcaggtgccg ttccaattgg ataacgggct aataccccca
    94621 ttaaaacaca gccgatcgca ctggctaatg cagtcgcagt aaacactgct ccttcatcca
    94681 ttccagaagc acctaataca gttggattta caaataagat ataagccatt gagataaatg
    94741 ttgtaaatcc tgccagtacc tcacgtttta ccgtggtgtt caatttttct atttcgaagt
    94801 aggaaagtat tttttccttc atcttccatc cccctaaatc cgaacattat aaataaagtc
    94861 cgttaaaatt tcgatttatt aataaaacaa cgtgttttat catatactaa aacaaaacat
    94921 aagtaaacaa aaaattcgga ttttaagcaa aatccgaacg ttccatctga ttaaatccct
    94981 ttatctcaaa gttcatgaac attcactttg gattacaaga atttctttat tgtgttaata
    95041 aagtttcttc attctagtct caaaaatcaa tagtgtgatt tgattttacc atttcgtttc
    95101 tgttcttctt tgatgaattc ttttgtatcg acaggattca gattatttag tccgccaaaa
    95161 cctgtatcga ccatttttcc ccagcctttt gtcttctttt ttcctgattt gagattcttt
    95221 gctcgaagct tctcactatg ttttgctcgc cgatacttca aataaccttc taatcttccc
    95281 attagatcca ctgtttttcc tcctttttat tcaatcagac gactcagctt ttcctataac
    95341 aaaataattg ccttcagggt cagaaaaatt gaaagtcaag atgccattgt tatctgtgat
    95401 ttctcctggt tcttccatgt tctcgtagat agtttcaaat tcatccgtaa aaaatactaa
    95461 agaaggcggt ggtcctaata cctctggtga aaacttctgc acaaatactt ttgggaatac
    95521 gcctaattct aattcttttg agatgctgag tacaatactc ttaaattctt ctggcaattc
    95581 gattgtttca gaaatttctg caccaatttt ttctacaaag aatcggcaaa tcatctctac
    95641 gtcattgaca taaagcatca cacgtgtttt ttgtatcatc atttgaccct tctttctatt
    95701 tgattttctt tcctgtctat aaactgattc cattatatca cgaaagcgca aacaccttgt
    95761 gtttgatact attcaagaaa aataaagcaa tagtttctag gattgctttc ctccacaaac
    95821 tttttttaca aaaaaaaaaa acggcaatga atgccgctct tcttatttcg attcttttat
    95881 aattctgctt ctgattcatc tttggttagt gtgtgtctaa cactagttac gccaaatgtt
    95941 tgatcgattt tttccttcaa cgcttcgatt gcctgtgcgc cacgtgccgc ttgttcagca
    96001 ttgatggatt caatgaccag caccgctgct tgtgtttctt ccgtcagcat tgttctttgg
    96061 gcttcacgcc agttgagaca gcgacagatc gctccttgtt catcatagta gatgatttct
    96121 tccggaagtg ccggagcatc ggaatctgca cctaaaggca ggaatgattc gccaccttgg
    96181 gcttttccta aatgcagatt tcctgcgatc ttttcgatat cttctccgcc gcaaggtacg
    96241 ccaaaagaca aggaaatact attatagata tctacaagcg gattgattgg gaaaaattcc
    96301 cgttcttgat ttgcacgttt cagtaaagct tcaatagaag aacgtgcacc ttttttagtc
    96361 ttgaatgtgg taaatgcctg acgccactga gcaatgactt cgttttggct gaatgtttct
    96421 tctgtaaggt aatttcttgc ttcttttgct gcctcattca ataattgatg atacgtttct
    96481 tcactttctt ttgttacgtg gttatcgata ccatttacgg ataagatttc gatggttgct
    96541 tcaggaaata cttcccagaa attggtatcc actattactt ttttcatctg ttcacacctc
    96601 ttgattagaa taattgcttg atgaatagat agtctgtttg gggatcatct cccatataaa
    96661 aaacatgttc gctgtatttg ataaatcctt ttttacgata aaaagcgagc gcaacatcgt
    96721 tgtgttccca aacaccgagc caaatggctg ttttttgctt ttcttttgct ttttcaatag
    96781 ctgtatctag taaaaatgta ccgatacctt gatgtttaaa tttttttcgt acatagattc
    96841 gctcgatttc taaaaaatga tcagccattt cctcagattg actcgttcct gtatttagtt
    96901 tcaggtaacc cgcaggctct tgatcgatac tcactaggaa aaaatcggtt tctttatctt
    96961 ttagctcttc tgtaagtttt tctagtgcat aagcttgtgt caagtaatca gctaaatgct
    97021 ccggtgaatt atcttttcca aatgtatctg taaatgtttc gatactaatt tgttgtagta
    97081 agtctacatc tgaaggcgtc atttttcgaa tttcgtattt attcacggct tcttcctttc
    97141 aatagatcct tttattgcct tttttgacaa aattccaatc ttcgctaaca ttcttgcata
    97201 ctttatttaa aagatattcc aattgttttg cttcttcgtc agaaagacca tttaatgcga
    97261 ctgcattgga gtagttgttt tctcgaatga tgaaaggata gatttctgct ccttttttag
    97321 tcggatagag tttcttgatt tttttatttt ctttatcttc caaacgttcg atcatcccat
    97381 tggattctaa tttcttgatt gctcgggcag tcgttgtacg atctactttg atcatttctg
    97441 ctagtttttc ttggatgatt ccaggatttt cgcaaatacg tactaaataa agatactgtc
    97501 ctcttgttaa atcgacttct ttgaattcga tattgctaat agaatctagc gctcgggcaa
    97561 tcattccgat gtctcgaagg atttcagcca tttcatccca cctttatttt gtttgtaaga
    97621 ctagtttacc tctttttgtt gcaaatgcaa ctattttcaa taaaaaaatc aagttctttt
    97681 ttaataacta gtaaagtata ttcttttctt cttttacgaa aaagctcaat cacaatagat
    97741 ctggtttttg ttttctaaaa taagtaactg tttcttcgtt gttcgctttt ctgatagctt
    97801 tggcaactct tctatactaa aaaatcccat gttggaagtt tcattattct caataaactt
    97861 ggcctcttca ccgtggatcg cacaagcaaa aacaagttta taatactgaa aaagctgtgg
    97921 aatatctttt tgtttattcg tatcaaaaac tgctcgcaac tgagtagttt ctactgttaa
    97981 tcctgtttct tcatagactt ctttacgcac attttcttca ggagacaatc ctatttcagc
    98041 aaatccacca ggaagtgacc attctttcgt ccgtttatct tcgattagta ggaccttgcc
    98101 atcttttttg atataggcac gaacatctac ttttggtgtt ggatgtcctt ctccaatcgt
    98161 tgtgagcgct tttatttctt cgaaagtttc ttttcctatt tcactgatca gttccaatga
    98221 aatcgtccgg atctcctcaa aacgctcttt atcaaataca tcttttccat aatatagacc
    98281 agcttgagaa agtgcgagta gtcttttgta ttgagccaac ttcttgttca ttctcttccc
    98341 ttctttttta gcaaatgtat gactatgtaa tcaagtatag aagactggtt taattacggc
    98401 aaataaatat aatgaaatct gcaataaata aaagaatgtt aaaaaactcg agaacaaaag
    98461 acattcgact tctctggggc tgtgaataaa tggcgttcaa aagcttgcat ctgcggtaat
    98521 aagccccacc aaacgaaaaa tatgacgagc tattcttgtc tggctgttct tattaattga
    98581 ggcaggacag ctttttcaca acctccccac taaacggtgt tctcctacca actcctcggt
    98641 cacaagtcac aatattcatc aaacatgaga agctgttttt ttgaaattgc tccttgtgac
    98701 tcggagctga acgacaggct cacgacctct cggtaaacag cgttcaaaag ccaatttttc
    98761 gatattaagc catttctttg ttacttattg tcattgattt ctttttctaa tttatccaat
    98821 agtggagtgt aatccacttg agtatagtct gttacatcaa attcatatag tgttcctaac
    98881 tggaatttgt catattttcg gtcggcgatg atctttttga atgcttcttt cgtaatatga
    98941 ttggtccctt tcgttcgatc ttccaatacg tcaccatagt gataatgatc catgatatag
    99001 ctcaaatgac gatctgattc acgatcacgt ttgtaccgac gttgacataa cacttcaaag
    99061 tctgctgtta ggcgaatcgt gatcacctga tatccgtatc tatctgctaa ttcttggagt
    99121 cgtgcttttt gcttatcact aaaaggatac tcggacacaa tcactttctt tcctgcttcc
    99181 atatacaaat caagtgcttt gtaatagaaa ggataaactt tcgtcctttc ttgttccgcc
    99241 cgttcttcca aattgttaaa cccgatggat tctgcatagt attccttgat ttcatcgagc
    99301 gtgatggcat acatatctgg aaatcgttcc cgtagtttat tcataagata tgtttttcct
    99361 gtaccaggac taccagccag aagaatgaaa tactttttca tctcatttat ttcctaagtt
    99421 aattggactt tccaagttca taaacagtcc aatcttatgc ttgacaattt cttttactgc
    99481 ttcatttgct ggaatcacat tatggcgaag agacttgttg atttctgttt cactaaaggt
    99541 ttctttagct actttcgtcc agttgactaa aagctccgtt ccaacattga atttgcggat
    99601 cccgttattg atcaattcag gataatcttc ttctttcaca cctgttcctc catggataac
    99661 tagggggaca tcaactactg catcgatttc tttcagcaag ggaatgttta cttctgtttt
    99721 tgatttgaat tgtccatgat tcgtaccgat agcaatagct aatgcatcca cgcctgtagc
    99781 ttctacaaat ttcactgcgt cttccggtct tgtatatacc ttgtcatttt cagcgacatg
    99841 gattccttct tctgtaccgc cgattgttcc aagttctcct tctacggaaa caccgtgcgc
    99901 atgggcatat tctactactg cttttgtctt caggatattt tctttgaaag gcagatggga
    99961 cccatcatac atgacagaag tatatccgct gtcgattgct tctttgatat catcaaagtc
   100021 tcgggcgtgg tctaagtgca agactgcatc aaccatttct tcttctgcaa tagctgtcat
   100081 tacattgact aacacatgca tgccgatata tttagcggta tcgacacttg tttgaataat
   100141 gatcggcgaa cccatttctt ttgctgcgcc tagcatagca ggaagcattt ctaggttatg
   100201 cgtattaaaa gctccaactg taaaattcaa ctcttctgct gtttttgtta cttctttcaa
   100261 tgttgtatac atcttttatt gccaccttat ctttttattt gtttcctttg atcgttactt
   100321 gttttgcaat ttgtcgcatc tcatccattt tgcccatgta gtaatcgatc ccttgatcat
   100381 caccagcacg agcagcatct gcacgcttct ggttatagag gctcatcagt tttttatacc
   100441 catctccgat cgtctgttta ttttccaaac tcttttctaa atattggatg gcttgatcgt
   100501 tcacatacaa ttcagaaaat ttaagtccaa cttgttcata aagtttcgct aatttatgct
   100561 tatcttgggt agtggaaatc tcttgtttca attgtgtaat ctctgattga atcttttcgg
   100621 tcgcttctgg cgatagggct ttttcttctt ttgccagaac ttttttcggt tctttttctt
   100681 ttttcttaaa aaatccaaac atcattttcc ctactttcaa ttaaaatgct ttcaaaactg
   100741 ggcttaagat ccaggcatat aagagtgctg cggctactgt atctacatcg gttgctgtag
   100801 cattgatgaa tcccatcgaa ttaaagatcg ttactagcaa agcgggcaat aatgtaataa
   100861 agaaaccatg agcaataccg ccaatgatcg ctcctcggcg tccgccaact gcgttgccaa
   100921 atatccctgc tgtccctccg gcaaagaaat tggttagcat accaggtaaa atcatcgcta
   100981 agccgaacat gggcaaagta aacatggcaa taatcgtacc aatcgttgtc gtaatgaaac
   101041 ctaaaatgac agcatttggg ctataaggaa agaaaactgg acaatctaag gcagggatcg
   101101 catcaggaac taatttcata gctatccctc gaaatgccgg cacgatttca cctaacagta
   101161 gacgtacacc agctagcaat acatacacac ccactacgaa ttggatggct tgtaaaaagg
   101221 caaacatgat atagttttga tctcctgata attcagaagc gaatggttcg cctgcaaata
   101281 aaacagtgat gatatatagc gggaccatca cgaccatgac agacaaataa gtatcttgca
   101341 gaaattcgaa atgtgtcggc aacttgatat cttcgatcga ctttttattt tcgcctttct
   101401 caccaaaaag cttagcaaca ccggcttcaa ataagtaccc gatcgtacaa aaatgaccta
   101461 aagcaatatc atttgatcca gtgattttac gaatgatcgg ttgcgctact gccggcatag
   101521 caatggcgaa acaagctccg ataaaaccac cgaccaagat caatacgatc cctcgcagac
   101581 cggcaaaata gccaaagact gtcgtcatcg ttgccatcca taaaattgct tgaccagtta
   101641 aaaaaatgta cttccatttt gtgaaacgag ccaaaataat attgaataca aaaatggcta
   101701 agaacgtcag tgcaatatct ctacctaaac ccaaatcatt cattgcttga ccattgatag
   101761 attcaataga agggatgatc ccttgcatat gaaatccttc agtaaagatt ttcccgaaat
   101821 aagttaaact tccaacgatg atgctcgaac cggcactcaa cacttgaaat cctaggagtg
   101881 tctttaacgt gccagagatg acttgtcctg ctgatttctt ctgcagtagt agacccaaca
   101941 tagcaatcaa tgcaatggtg atcgatgcct gagtcaaaat attttcgatg ataaaattga
   102001 taacgcccat gtttttaccc tcttttcttt ttgtatttag ataactccct tatcttgcag
   102061 aactggggtg attttttctt caatttcatt ttttgaaaca atatttttca aatagatcat
   102121 cgccgtttta ggatcaactg cgaacttttc gaactgcgaa cgaaaatttt ctgctgtgat
   102181 gatcacatct ggtttcatgg aaactgcgga agaaatatca caatggtcca gtttggcttc
   102241 tactcctaat ttgttcaaaa catcctcagc agacatctgt gctgcaaaac tacttcctaa
   102301 acctgcaccg catacgaata aaatgttcaa tttgttcatc tttgttctcc cccgtatcat
   102361 ttattatttc tcaactactg aaaaaagcag ttgtttgaaa tcttcgatat ttgtgcattc
   102421 aatcaatcga tccaacttct cttcgttgtt gatcaattca attagttctt tcatgatgtt
   102481 cagatgcgag taggagtcta ctgctgccaa acaaaaaata atctttactg gatccatttc
   102541 aggattaccg aaatcgattg gttgctcaat cgtagctacg ctgactccta atttattgac
   102601 cccatcttct ggtcgggcat gggctagtgc catgtgtttg ccaatcacga tatacggtcc
   102661 gtaatcattc actgcacgaa tcatcgcttc gacatagcgc tcttcgacaa tgttttcttt
   102721 aaccaatggt ctagcagcta gttcaatcga ttcctcccaa gtagtggctt tttctttgat
   102781 catgatattg ctgtccgtca aaatatcttt taacataggc tggatctccc ttttgttgat
   102841 ttctaaattg tttgtttcga ataatttttc taacttgcca tagatgattc gatctactcg
   102901 gccaccactt tctttcatga catctattat ttgagtgaat aactccgtcg attcatgact
   102961 gttaggctct aaccgctgta gttcttcatt tcttttcaaa aagtcttcta taaccaagat
   103021 attcgtctca gttaaaatcg gatcgacaac taaaagcggc ttatttcgat aattcaattg
   103081 aaaggtgctg aaaaccagat tgacatccaa tttatctact aattcgattt cttttgaact
   103141 taaaactgca gtaatctcga tctgaggaaa cttttctttc agattctctg ctaacagatt
   103201 tccagtagcc atcccatggt tacagacaac gactgattta taaacataat tcaagtctcg
   103261 cttgattaca ctggcgatcg tcgaaaagtg gatgacaaga aaagctatct cgtcctccag
   103321 tatcttgcca ccaattactt cttcgatcgt aggaacaaaa tgagtgactg cgcggtagat
   103381 tgggaaataa tttgttcgga tgttttcttt caatggattc atgacttgga tatgattctt
   103441 gattcttaca atcagtgcag ccatgtgctt atagagattt tcgaataaag cttcttcctt
   103501 tagatggaaa ggtacgtgtg tctcgttctc tacaaattgg atcaattgga tcgttagtaa
   103561 ttgagcttgt acccactcga ttgaattgga gatatctcta gcattgaacg tctcaatagt
   103621 gaaacgaaga taatttcttt ctacttgcgg gacattgtcc aattgaaatt cctcgatcat
   103681 gtggtcaata aatgcagaaa agctactgtc ttttatttct gtatttttcc aattaatggc
   103741 agagataaat tcatgtttcc tgattcgctg gatccagatc aatgtaaaaa gcaggatttg
   103801 ttttttataa atatcatctt cttgtttcaa caacttattg gaatagatct cctgtatttt
   103861 tttgatttcc accaaaggaa aataatggag aaagattttt tgagccggtg ttaattgtgc
   103921 atcttgattc ataaagaagt cggtcattcc cagattctga ttgataaaat catagatcat
   103981 cgtacggata gatcgttctg aacctttata aaccagtcct tgctttccat aactgacgat
   104041 ctctatccca taactgacca gtgttgctcg taaccgtctc atatcttcat ctaatgtact
   104101 tttagagatt tgaaattctt cttctttttt gtttagatgg atcgggattc ggctgtaagc
   104161 aacggctaaa actagatcaa aaatacgttc gcgccgattc aaaaagccgt ctttcacatc
   104221 ttttatttca gtttctaatc gagcttgctt ttccttttcc agcttcaaca aaaaaacttt
   104281 gttgcgaatt gtacggatct ccggaagctt tttactgctc aaccagatat tcgcttcaag
   104341 aacttcctga cggatcgtcc gctgactgac attgaactca cttgctagat gttttgcttt
   104401 tactggatat tgggctgtca tgattctgtg aagtatttca attgttcttg ttttcataga
   104461 aatgatctcc ttctgatttc aagtatatgg ttccgcttac ataataaaaa gtagatattt
   104521 ctgccacttg aagtggaaag aaagtaagca aagcaaacaa aaaataagcc atatgctttt
   104581 tcatctcctc aacgaaaaag acatacgcct attattacaa aaattttgcc catctataca
   104641 aaaaagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaacaacta aactaaaata
   104701 gtgcttccta tttttcgttt ggttgttctt attatcgcag atgcaagctt ttaaacacca
   104761 tttagtggag aggttgtgac agagatgttc agccttgagc gataagaagc gattttgaaa
   104821 aatcgcttcc catattttac aaaatcgtgc cttattgccg ataggctatc tctggcacac
   104881 tgtttattca gaaaaaggag ctgcgacaag gcttttgtca caactccgtt cataaatatt
   104941 tcctgtcaat ccagcataat cggctgaata aaatcggctt gccatctttt ttctttctca
   105001 gaaaaatcaa aataccatat ctgagaaaaa tactgtcgtt gtgtcttact tcctgagatc
   105061 aatcgcccat cttttcgatc gactgaataa tcacagcatc tgaaatcaat tcggatggca
   105121 aaactatttt cgtggatctg tcgataattt cctacttcct gaatagttat ttttttagta
   105181 tgatttctta caccgtctgc tttgtttttt tgcagaactt tctgatgttc ggcaaacagc
   105241 ttgtctgtat aacagcttct ggctttttca ggagtttctt cttcccaagc ggattggatc
   105301 ttcatgaata tcttttggat ttccagaaac tgtgctttct tttctttagc cgttcctgac
   105361 atgcgggaca atatctccgc tttcagcctt ttcttttgtc ttttattctt gacgatagaa
   105421 aaagcagaaa cccctcctac tgacaaaaca gctaatgcaa gaaatcccgt ttgctcataa
   105481 ctagaaggac ctgtacctgg accataatac ccataagaat ctctgccgta agaacgactg
   105541 ccaaaataac tgcccccata tgaacgacca ccaaatgaac ctctgcccga acttcgaccg
   105601 ctagatgtac ctccaccatg accacccgcg acagcgaatc cttgggtcgg cagagcaaac
   105661 aaacataggc ccactaacaa aatgattgct aaataattga tttttttcat tgtcttcctc
   105721 gttatctttt attacaaaaa actattttta tgatagcctt gctaaaagga agagtcaata
   105781 accagatgca taaactgttt ttttagcaat tttgtcttat ttagtcattg agtaacatcg
   105841 gacaaatgta tgtgtcggca gtccataatt cctctatctt cagcatgaac aagcttgcag
   105901 acaagagatc tgcgcttcct ccaggagaga tttggttttc ctgacaaaag tgcaacagtg
   105961 ctgtaaatgg acgatttggt aaagcacttt gactagcagc atgagcaagt ttttgcattt
   106021 ccactaacgt tttctgatca ctgcgatgca atacgcatgt gtcatctagc gaactcatca
   106081 aaactaacaa catatgcatt tgtttctgta catatgaatc attatctagc tccttagctg
   106141 cttggattgc aagcctgata tgcggatacc ctaatatcgc ttcttcatat gcaccattta
   106201 cgccatattt ttgcttcacc ctatgaccat gagtggtttt tttatgacga tattttgtat
   106261 caggaaaagc agcaagcttg ctgacatcat caagtatttt cgataaagaa aaaggctgtt
   106321 ccatactgat tttttgtgca atgacactga tcaatagccc cattgcccaa atcgctcctt
   106381 tatgggtatt gatgccattt gtagcaatca gcattgcctt ttccgctttt cgcccaatct
   106441 cagcaattct ttctcttaac tcctgatcaa tagcatgatg ccatgctatt tgagccattt
   106501 cttcaaaaaa aggagttagc gtcttagcag acttaagaaa taaagcatag tccatatcgg
   106561 aatgtgcacc cgcatccagt gcatccacta atccaggttt tggacttaat gttacttctg
   106621 cgatcaatgc ttcctctacc aacgcagcga gttgctgagt cactgctttt tttcgttcgt
   106681 catttatcat gacactcact ccttcttcct actattactt gctgaaacct tttttgatag
   106741 ctacccaaat gatatgaagt aaccagctta aagcaaatgc acaacttgcc gtcattagat
   106801 aagctacaag aatcatgact cctgaaggta actgcagcca actatcttta aacaataata
   106861 acagtacttg gaaaacaaat acattggcta aatacgtacg ataagcataa acagacaacc
   106921 atttgaacaa tggcaatttt ggcgaatcgt ttacaatcat tttggaagcg cctatgaaga
   106981 cagcaaagat gatgactaaa ctgtataaac tttggatcgt attcaagtaa ggtgcattcg
   107041 caaagctcaa gtctcctgga tgtgcgagca atgaacggac agaaaagaac gcgataacta
   107101 aggcaacagg taagaaaaga taacgtactc tgtccaaata ttgatagact ttttcgtgat
   107161 aaacaaatgc ggctacccca taaatcatga aactagcaac aaaccgatct aataagtacc
   107221 agctgtcatg ataaggccca gtcaatactt gtgtttgata gaacagatac catgcgacat
   107281 ataaaatcgt tgaaccaatc aatactggcc atacatattt tttattagca aatactttgt
   107341 aaccgagcca aacaaaaaac ggcatgaaca attggatctg cagcatcata acggtatacc
   107401 ataaatgagg cgctccatca cctgtgacat atttcagtaa aaagcttgca gtatccgtat
   107461 acggagtttg ttgttggact gctggaaata gccaaagata agccgtcgtc catagaagat
   107521 acggaacgac tagttcaaaa aacttttctt tcaaaaattc ccaataagac aaatgctgac
   107581 ttgtttttac catattataa acaattgcaa aaataaacat tggtgctgtg tatttaacga
   107641 tgacgtaaaa ggtaccaaga aaagctgtat ttgttgcgct gtggctgttt gccaaagcaa
   107701 agctcaatat cgtttgcatc ataacagccg ttacagctga tactttctgg aagtcactta
   107761 cgttgatttt gatttttttg ttttcaaaca tctctttctc tctctttttc tttattacca
   107821 aagttctttg gttaatttag gtccttcgac tgttttcaat agaatttttt tctctggctc
   107881 tctcaaatac tcttttagtg aaaaaccgcc ttgccgagtc actacttggg tatcgaattg
   107941 ctcaaaaaaa gcaggatgcg attgaattac ctgaattggc aattcaatcg gtgaatttgc
   108001 gtacagcagc aaatcaaaat cactggtttc ttttactgcc ggaatcccag tcgataattc
   108061 aaatcctaag ctgccgccga ttccccattt acatcctgat aatatgtttc tagcagcatg
   108121 aaattgctga taaacaggat aatccatgat tttctcttgt ctatacgatt cttgttcgat
   108181 gatgtcccac ggatgaatga cttgtttgac ggcatgtttt gagatttctg ttgaccatct
   108241 ctgattgcgg gcaggtccac gtaaaccaat cggtagtatt tctgcgtgat ccgtcgacgc
   108301 acgacgaact gttccgaaca tcttttcagt tagaacgacc cattcaggca aggtgaaccc
   108361 ttctatttca tgtttatcaa aaaggacaat atcatgtgct tgaatcattt tttatgcatc
   108421 ccattcttcg tccattttgg cacggacttt atttgtttcg acacgtccga atgttttggc
   108481 tgtttcgttt gtgtatcgat ttgtcagcat cgtgtcattt gtattgcgga catcttgaat
   108541 ggcttcattg attgcttcga tgactttgct gacattttgt tctgaagcag cttggtcggt
   108601 gatatcttct aggaaatgat agatcgctcc tagttttgta taattattga tatcgtaagc
   108661 gatagctgga actttttcag cagctgcttc gatttcagca accgaacgtt ttgtcacacg
   108721 tgcagcactt tctttagaca tcgcttggac ggtaatttct ttattgttta aagcaatcaa
   108781 gcggcttgat tgtaatccat gtgctaaaaa tgcacctgaa atagcatttc ctgcaatcaa
   108841 tccaataact ggatgtccag cttgacggag ttttgcataa gcagcagcac tattagccaa
   108901 agccacatgg ataccgatca attcttcgtt atagccgtat gcttggcttg gtacatcgat
   108961 cacaacgatg attggccgtt tcactttttt gtctttatct tcttcgtaaa cgtgatccaa
   109021 aatttgtccc atgcggaaac cttcaactaa accaacttca ccgtttcgta cacggtacat
   109081 cgggttttta ggatcaggta cgattgatgt cacgatggct tcttctatga atttacttga
   109141 tttaccataa tacacagtac taatagaaga agttggattt ttcatgccag ttagtgccgc
   109201 aaaccaatcg taaccaacac ttgctgcagc tggttcattt ccttctgcta cttctggtaa
   109261 gtcaattgat tttgcttgat ggttagcaat tgcttcattg taatcgtcaa tggccattgg
   109321 ttttgaaaga tctaaagaat caagtaatga taaataaaat gctgagcttt cactgcgatg
   109381 cgtgtctttt cgttcgtcca ttgctgcgat gacagcagct ttgattgctt ctacactatc
   109441 agtcactaat tcatcaatga tttttgattc tacccgttga cgagatccta atgtattcca
   109501 aatcaagtct ttgtcactag aatcaaattc tcgaacgcct gcttcttgtt caataacttc
   109561 tggtccattc aaaccgactc tcgctttttt tgttgcgatt aaataactca ttaatgctga
   109621 agtaatcgac ataccgccaa atgatcctac acgtcctgga atcaaaccga tgactggtac
   109681 atattgtttt agagcaatga ttacatttcc gatttctgaa atagataaca gtccgtagtt
   109741 tgcttcttgc aaacgaacac cacctgtatc tagtacaatg actggaaaga tctcatttcc
   109801 ttttttattt tcttctaaca catgttcaag tgcagcaacg atttttgcac cggaaacttc
   109861 accgataccg ccaccttgga attttccttc catagcgatt actactgctt gtttgccatt
   109921 gatcgtgcca cggctgacaa cgatcccgtc atcactttca gggacgattc cttgtgctgg
   109981 taaacttggc gcgatgattt gatcgaatgg atcaatcaat tcacgtccat tgtcaagtag
   110041 agcgttcact cgttctctgg cgtttaattc tacaaaacta tttttcatct gaaagttcct
   110101 ccattgcttg cgtcaatcgt aggtacacaa ctcctggtgt agcaccgaag tcattgatca
   110161 cgatatctgc tgaaattgga taacgatcaa aaaatctcgt taatacattg ttccatactt
   110221 cgccgaagcc atcgcttcct gtacaaattt ttacagtaga ttctgttttt tctgttggaa
   110281 acatcaagat ttccaaatct cctgaaccga ccactcctac atggattttc ttattgatcg
   110341 gctgtttcgt tggatattta taagctaatt gttccatgga tctctcccct ttttgtaaaa
   110401 cagagcatac acgttctgct tattcgtctt tcttcttcca tctgggtaaa tggcgacaaa
   110461 aggtttttct cgccaatttt atgaccaagt tctgaatttc attggtggtt catacaggcc
   110521 atctgaccat tcgatcaaat catcgatggt ttgtgcagct aataatgaac gtttcgcttc
   110581 actacgtttg atgcctagat cttctggtaa tttcacgatg ccggctgcac gcaagctatc
   110641 tagtgattct ttcgtgctag tcattccaag aggtgtaaca ccgccaattg ctgcaattgc
   110701 tgcttgtcgt tcttcttttg tttttgcttt gtacaaataa gcaattcctt cttctgtcac
   110761 gatatgtgta gtatcttcgc tataaatcat cactggtgca ttggctaagc ctgcttgttt
   110821 ttgaacagct agtgcatcta attcttcgac aaagactggt tttttatttg aaccatatgt
   110881 ttcaaccatt tgaacaacta atttttggcc tttccctaat ggatcatcat tgtcgcgaag
   110941 tgattgccat gctggtgtag aatgacgacg accgccagga ttatgtccca tgttaggcgc
   111001 accgccaaaa ccagataaac gtccttttgt tacggtagaa gagtttcctt cgtaatctaa
   111061 ttgcaaggtt gaaccgataa acatatcgat ggcatattgt cctgcagctt gtgataatgt
   111121 tcggttactg cgcatcgtgc cgtctttacc gacaaaaaag acatctgggc gagcagcaat
   111181 atatttttcc ataccaactt cgccaccaaa actatggatg ctttcaaccc aacctgattc
   111241 gatggctgga atcatcgttg gatgtgggtt caatacccag ttcttcgcga ttttcccttt
   111301 taaccctagt gattcaccat aagttggcag taacaattcg atagctgctg tattgaatcc
   111361 aataccgtga ttcaaagatt gtacgccgtg ttctgcgtag attcctttga ttgccatcat
   111421 tgccattaga atttgcaatt ctgtgatgtt ttgaggatct cgtgtgaata aagcttctaa
   111481 ttgataaggt ttatctgctt tcacgacaac gtctacccag tcaccaggga tatctacacg
   111541 tggtaaagaa tcgacaactt cgttgacttg aacgatcacg ataccatctt tgaaagctgc
   111601 agcttcgatg attgttggtg tttcttccgt attgaaacct gtgtacaagt tgccgtcttt
   111661 atctgcttta tctgcagcta ctaaaacaac atttggaatc aaatcgatga ataagcgtcc
   111721 ataaagttcc aaataagtat ggatatcacc taatttcatt gtgccgtctt caatcatttg
   111781 ggcgacacgc aagctttgtg caccagcata agagaaatcg atcttgcttg cgattccttt
   111841 ttcaaaaata ttcaaatgtt ctggtcgtga gatactagac atgatcaaat gcagatcatt
   111901 cactttttct gcatctactt tttcaagact ttctgaaaga aaacttgctt gtttttgatt
   111961 gtcgccttct aatacaactt tgtcgccggg agcaatcagt ttttccatta cttctacgat
   112021 tttttcagtt tctacatact tgccattgag caagttgctg attttttcca atcgctgcgc
   112081 ttttttttcg cgtttagaag cccagttgcg gtttggtttt tccataatta gtcatctcca
   112141 tttttgatat agaaacgtgg cgtcatcaat accatgttgg tctagattga tcgtacggat
   112201 ccctttatct acaccaaatt ttgtatgaat cagctttgtc aaagtattgc cggggggaaa
   112261 ttcaatcgtc agatccattc ctaattcttt tgccaagtcc atcatttggt tccactgcac
   112321 agggaaagct aggttttcca acaaatcttt acgaatctct tctgcgtcct gagtggctct
   112381 tccatccgta ttttttatat acaaacatct agcatcagat agatggacag actgcatata
   112441 cggcttgaat ttcttgaccg tatcattcat caagagacag tgagatggca cagggacttt
   112501 cagcagcttc gctgtttgcg cttggtgccg tttggcttct gccaacgtaa cggataaacc
   112561 ttcgatactc ccagataacg tatgttgcat cggacaattt tcatttgaca gataaactgg
   112621 atggtcagga tcaaatgttt ttgctacaat ttcctcagct tcgcttctcg tcacacctac
   112681 tactacgccc attgcataac cgcttggata agcttctttc attaagactg cgcggtgata
   112741 gactagttcc gctgcattct caaatctcaa agatcgacaa gcaacagctg cacaaaaagc
   112801 accaatcgaa tgtcctgctg caatatcagg ctgaataccg tttttttcca tttcatctaa
   112861 ataaaatact gcttttgtca gtaatgctag ctggataaaa accgtatctt gatagttttc
   112921 ttctttatca actaaccgat accccgtcac attttctaaa cgttccatgt acatttcagg
   112981 gatatccgct aacatcccta acttctgact gccttgtcca ggaaataaga aacctgtact
   113041 catgagcctc gcctcctcag cacttacttt ttccactgct atcttagtca atttctgcta
   113101 taataaaaaa tgccaaaaaa agaaaaaacc ataacaaaaa ggtatggatg ggatggataa
   113161 ttatgaaatt gcttcatctg cagtatttta aaaaagtatt cgaaacaaaa aatgttaccc
   113221 aagcagccaa agaattattt atttctcaac ctgccttgag ccgtgcgatc aaacatttgg
   113281 aaaacgaatt aggtgtccct ttgttttacc atagcggcag aaacattgag acgaccgttt
   113341 atgcagaaga attttatcca tatgcagtca agacattaga cacattagaa gaaggaatca
   113401 aattattgtc ttcggtcaat gagaagatcg ttacttccgt catattgtat ttagaggtag
   113461 cctctgtttc tattccgaac ttggtccgaa tttttatgga aaagcatcct gatatccaat
   113521 taacgatcat gcaacatcat ttaccagatg atattaccga tcctgtactg tacatcactt
   113581 cggagatgaa gcctggcatg accaatattc ctatcatgaa agaacctgtt tatgtcgcca
   113641 ttcctaagaa acatcctctt gctaaaaaag aacatctgca attagaagat atccaacgca
   113701 cccccatctt gatgctgtca aagagcaacg ccttccgtca tacactggat gcagcacttg
   113761 aagcgaaaag tatcgaattg acgatcagtt cgacgacaga tgatcctgcg acattacgtt
   113821 ctattgtcaa gcaaggatta gctgttagct ttttcccaaa agtttcttgg tcttacgata
   113881 aaaatgatcc tttcgtcttg cgggaaatca aagatttgcc cttgacacga acaatttatt
   113941 tatcttctag cttcacagaa gatcatcctt tgatcaaaat gattgcaaaa acgatgcggg
   114001 aattttattt aggaaaataa aaaaaaaaga gctgggacaa aattcttgtc gcagctcttt
   114061 tttcgaacaa acggtgttcc agaagtaatt tcttagaaaa taagccgaaa tttcccaaaa
   114121 atttgagaag caattttcgt aaattccttc ttatttcttg aagctaaaca tttctgtcac
   114181 aacctcttct ctattgtcct agacattcat tgcgttgatt taaaacttcc atcaatcaaa
   114241 tctgctagtt catctagctt ttctatttta cgtagatcaa tagagatcca atgttcttta
   114301 ttcatgtgat aggctggaaa atacccctct tttcctcgca aaattccgcc aagttcagga
   114361 tcgaccttca aatcaagaat attgatgatc ctatggtcac ttgcatcatc atagattttt
   114421 tcttctggaa cagacatcag gaccccaaac cattttttgc ttttttgatg tctgaatacg
   114481 caataatcag gaaatttttt aaaaatatat tctggagaca aattgtatgt ttcattgatg
   114541 tgatcgatta gttctttttt cgttagcatc tgcttccatc tctttcttat tgattcgctt
   114601 tattcaaaga taccacgtaa gaatagaaaa atgaataca
//
LOCUS       DACDSY010000002        80066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDSY010000002 DACDSY010000000
VERSION     DACDSY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.002"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..135
                     /locus_tag="IWK44_000102"
     CDS             <1..135
                     /locus_tag="IWK44_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HAP9548661.1"
                     /translation="SKEITSMLLISIFFVALYGSLTSDRWILLKQGGLALLALLSIFL
                     "
     gene            258..1889
                     /locus_tag="IWK44_000103"
     CDS             258..1889
                     /locus_tag="IWK44_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP9548662.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1951..2592)
                     /locus_tag="IWK44_000104"
     CDS             complement(1951..2592)
                     /locus_tag="IWK44_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9548663.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            2874..3182
                     /gene="rplU"
                     /locus_tag="IWK44_000105"
     CDS             2874..3182
                     /gene="rplU"
                     /locus_tag="IWK44_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP9548664.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            3209..3553
                     /locus_tag="IWK44_000106"
     CDS             3209..3553
                     /locus_tag="IWK44_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP9548665.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3566..3859
                     /gene="rpmA"
                     /locus_tag="IWK44_000107"
     CDS             3566..3859
                     /gene="rpmA"
                     /locus_tag="IWK44_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP9548666.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4266..7100
                     /locus_tag="IWK44_000108"
     CDS             4266..7100
                     /locus_tag="IWK44_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9548667.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            7291..7782
                     /locus_tag="IWK44_000109"
     CDS             7291..7782
                     /locus_tag="IWK44_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP9548668.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7885..8874
                     /gene="manX"
                     /locus_tag="IWK44_000110"
     CDS             7885..8874
                     /gene="manX"
                     /locus_tag="IWK44_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP9548669.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8907..9710
                     /locus_tag="IWK44_000111"
     CDS             8907..9710
                     /locus_tag="IWK44_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP9548670.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9731..10645
                     /locus_tag="IWK44_000112"
     CDS             9731..10645
                     /locus_tag="IWK44_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP9548671.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10728..11102
                     /locus_tag="IWK44_000113"
     CDS             10728..11102
                     /locus_tag="IWK44_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP9548672.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11241..11486
                     /locus_tag="IWK44_000114"
     CDS             11241..11486
                     /locus_tag="IWK44_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9548673.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11658..12722
                     /locus_tag="IWK44_000115"
     CDS             11658..12722
                     /locus_tag="IWK44_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9548674.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12968..13393
                     /locus_tag="IWK44_000116"
     CDS             12968..13393
                     /locus_tag="IWK44_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9548675.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13386..13835
                     /gene="nusB"
                     /locus_tag="IWK44_000117"
     CDS             13386..13835
                     /gene="nusB"
                     /locus_tag="IWK44_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP9548676.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            13932..14786
                     /locus_tag="IWK44_000118"
     CDS             13932..14786
                     /locus_tag="IWK44_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP9548677.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14787..16124
                     /locus_tag="IWK44_000119"
     CDS             14787..16124
                     /locus_tag="IWK44_000119"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP9548678.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            16129..16353
                     /locus_tag="IWK44_000120"
     CDS             16129..16353
                     /locus_tag="IWK44_000120"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP9548679.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16355..17245
                     /locus_tag="IWK44_000121"
     CDS             16355..17245
                     /locus_tag="IWK44_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9548680.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17254..18072
                     /locus_tag="IWK44_000122"
     CDS             17254..18072
                     /locus_tag="IWK44_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP9548681.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            18179..18628
                     /locus_tag="IWK44_000123"
     CDS             18179..18628
                     /locus_tag="IWK44_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9548682.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18646..20328
                     /gene="recN"
                     /locus_tag="IWK44_000124"
     CDS             18646..20328
                     /gene="recN"
                     /locus_tag="IWK44_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP9548683.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20383..20499)
                     /locus_tag="IWK44_000125"
     CDS             complement(20383..20499)
                     /locus_tag="IWK44_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP9548684.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20572..21528)
                     /locus_tag="IWK44_000126"
     CDS             complement(20572..21528)
                     /locus_tag="IWK44_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9548685.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21699..22067
                     /locus_tag="IWK44_000127"
     CDS             21699..22067
                     /locus_tag="IWK44_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP9548686.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            22237..22668
                     /gene="mraZ"
                     /locus_tag="IWK44_000128"
     CDS             22237..22668
                     /gene="mraZ"
                     /locus_tag="IWK44_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP9548687.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            22689..23648
                     /gene="rsmH"
                     /locus_tag="IWK44_000129"
     CDS             22689..23648
                     /gene="rsmH"
                     /locus_tag="IWK44_000129"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP9548688.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            23653..24054
                     /gene="ftsL"
                     /locus_tag="IWK44_000130"
     CDS             23653..24054
                     /gene="ftsL"
                     /locus_tag="IWK44_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP9548689.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            24055..26247
                     /locus_tag="IWK44_000131"
     CDS             24055..26247
                     /locus_tag="IWK44_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9548690.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26273..27235
                     /locus_tag="IWK44_000132"
     CDS             26273..27235
                     /locus_tag="IWK44_000132"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP9548691.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27249..28610
                     /locus_tag="IWK44_000133"
     CDS             27249..28610
                     /locus_tag="IWK44_000133"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP9548692.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            28636..29724
                     /gene="murG"
                     /locus_tag="IWK44_000134"
     CDS             28636..29724
                     /gene="murG"
                     /locus_tag="IWK44_000134"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9548693.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            29739..30959
                     /locus_tag="IWK44_000135"
     CDS             29739..30959
                     /locus_tag="IWK44_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP9548694.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            31115..32440
                     /gene="ftsA"
                     /locus_tag="IWK44_000136"
     CDS             31115..32440
                     /gene="ftsA"
                     /locus_tag="IWK44_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP9548695.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32463..33704
                     /gene="ftsZ"
                     /locus_tag="IWK44_000137"
     CDS             32463..33704
                     /gene="ftsZ"
                     /locus_tag="IWK44_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP9548696.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            33721..34398
                     /locus_tag="IWK44_000138"
     CDS             33721..34398
                     /locus_tag="IWK44_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP9548697.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34412..35011
                     /locus_tag="IWK44_000139"
     CDS             34412..35011
                     /locus_tag="IWK44_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP9548698.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            35026..35313
                     /locus_tag="IWK44_000140"
     CDS             35026..35313
                     /locus_tag="IWK44_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP9548699.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35362..36144
                     /locus_tag="IWK44_000141"
     CDS             35362..36144
                     /locus_tag="IWK44_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP9548700.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            36165..36872
                     /locus_tag="IWK44_000142"
     CDS             36165..36872
                     /locus_tag="IWK44_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP9548701.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            37134..39932
                     /gene="ileS"
                     /locus_tag="IWK44_000143"
     CDS             37134..39932
                     /gene="ileS"
                     /locus_tag="IWK44_000143"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP9548702.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(39981..41507)
                     /locus_tag="IWK44_000144"
     CDS             complement(39981..41507)
                     /locus_tag="IWK44_000144"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP9548703.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(41522..42169)
                     /locus_tag="IWK44_000145"
     CDS             complement(41522..42169)
                     /locus_tag="IWK44_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP9548704.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42353..42682)
                     /locus_tag="IWK44_000146"
     CDS             complement(42353..42682)
                     /locus_tag="IWK44_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP9548705.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            42859..43587
                     /gene="sfsA"
                     /locus_tag="IWK44_000147"
     CDS             42859..43587
                     /gene="sfsA"
                     /locus_tag="IWK44_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP9548706.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            43603..44616
                     /locus_tag="IWK44_000148"
     CDS             43603..44616
                     /locus_tag="IWK44_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9548707.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            44616..45893
                     /locus_tag="IWK44_000149"
     CDS             44616..45893
                     /locus_tag="IWK44_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9548708.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            45956..48658
                     /locus_tag="IWK44_000150"
     CDS             45956..48658
                     /locus_tag="IWK44_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9548709.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            48810..49127
                     /locus_tag="IWK44_000151"
     CDS             48810..49127
                     /locus_tag="IWK44_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9548710.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            49157..49477
                     /locus_tag="IWK44_000152"
     CDS             49157..49477
                     /locus_tag="IWK44_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9548711.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            49585..51045
                     /locus_tag="IWK44_000153"
     CDS             49585..51045
                     /locus_tag="IWK44_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9548712.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            51113..51334
                     /locus_tag="IWK44_000154"
     CDS             51113..51334
                     /locus_tag="IWK44_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548713.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51365..51547
                     /locus_tag="IWK44_000155"
     CDS             51365..51547
                     /locus_tag="IWK44_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP9548714.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            51547..51960
                     /locus_tag="IWK44_000156"
     CDS             51547..51960
                     /locus_tag="IWK44_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP9548715.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            52083..53264
                     /locus_tag="IWK44_000157"
     CDS             52083..53264
                     /locus_tag="IWK44_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9548716.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            53653..53726
                     /locus_tag="IWK44_000158"
     tRNA            53653..53726
                     /locus_tag="IWK44_000158"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53687..53689,aa:Arg,seq:cct)
     gene            complement(53763..53963)
                     /locus_tag="IWK44_000159"
     CDS             complement(53763..53963)
                     /locus_tag="IWK44_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9548717.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(54268..55500)
                     /locus_tag="IWK44_000160"
     CDS             complement(54268..55500)
                     /locus_tag="IWK44_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9548718.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            55754..56323
                     /locus_tag="IWK44_000161"
     CDS             55754..56323
                     /locus_tag="IWK44_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9548719.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(56501..56941)
                     /locus_tag="IWK44_000162"
     CDS             complement(56501..56941)
                     /locus_tag="IWK44_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP9548720.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            57099..57863
                     /gene="map"
                     /locus_tag="IWK44_000163"
     CDS             57099..57863
                     /gene="map"
                     /locus_tag="IWK44_000163"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP9548721.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            57895..58818
                     /locus_tag="IWK44_000164"
     CDS             57895..58818
                     /locus_tag="IWK44_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP9548722.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            58894..60033
                     /locus_tag="IWK44_000165"
     CDS             58894..60033
                     /locus_tag="IWK44_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP9548723.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            60026..60826
                     /locus_tag="IWK44_000166"
     CDS             60026..60826
                     /locus_tag="IWK44_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9548724.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            60826..61653
                     /locus_tag="IWK44_000167"
     CDS             60826..61653
                     /locus_tag="IWK44_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9548725.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            61631..62365
                     /locus_tag="IWK44_000168"
     CDS             61631..62365
                     /locus_tag="IWK44_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9548726.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            62465..63331
                     /gene="rfbA"
                     /locus_tag="IWK44_000169"
     CDS             62465..63331
                     /gene="rfbA"
                     /locus_tag="IWK44_000169"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP9548727.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            63345..63917
                     /gene="rfbC"
                     /locus_tag="IWK44_000170"
     CDS             63345..63917
                     /gene="rfbC"
                     /locus_tag="IWK44_000170"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP9548728.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            63939..64967
                     /gene="rfbB"
                     /locus_tag="IWK44_000171"
     CDS             63939..64967
                     /gene="rfbB"
                     /locus_tag="IWK44_000171"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP9548729.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            65065..65916
                     /gene="rfbD"
                     /locus_tag="IWK44_000172"
     CDS             65065..65916
                     /gene="rfbD"
                     /locus_tag="IWK44_000172"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP9548730.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            65951..67984
                     /locus_tag="IWK44_000173"
     CDS             65951..67984
                     /locus_tag="IWK44_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548731.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            68028..69308
                     /locus_tag="IWK44_000174"
     CDS             68028..69308
                     /locus_tag="IWK44_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP9548732.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            69518..70324
                     /locus_tag="IWK44_000175"
     CDS             69518..70324
                     /locus_tag="IWK44_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9548733.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            70336..71556
                     /locus_tag="IWK44_000176"
     CDS             70336..71556
                     /locus_tag="IWK44_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9548734.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            71546..73132
                     /locus_tag="IWK44_000177"
     CDS             71546..73132
                     /locus_tag="IWK44_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9548735.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            73171..75309
                     /locus_tag="IWK44_000178"
     CDS             73171..75309
                     /locus_tag="IWK44_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9548736.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(75677..76660)
                     /locus_tag="IWK44_000179"
     CDS             complement(75677..76660)
                     /locus_tag="IWK44_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP9548737.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            77038..78429
                     /locus_tag="IWK44_000180"
     CDS             77038..78429
                     /locus_tag="IWK44_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9548738.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            78445..79485
                     /locus_tag="IWK44_000181"
     CDS             78445..79485
                     /locus_tag="IWK44_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9548739.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            79504..>80066
                     /locus_tag="IWK44_000182"
     CDS             79504..>80066
                     /locus_tag="IWK44_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9548740.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRK"
BASE COUNT        26119 a        13544 c        16981 g        23422 t
ORIGIN      
        1 tctaaagaaa tcacaagtat gctattaata tctatttttt ttgttgcatt atatggcagt
       61 ttgactagcg atcgttggat tttactcaaa caaggtgggc tagctctgtt ggcattgctt
      121 tctattttct tataaatata gaaaaaataa gaatttatct aacaggaaac tttgtgaaaa
      181 atattacttt atctcatgaa atatgatatt ctaatgaatg gtattttaga aaatttcatg
      241 aaaaaggagt tagataggtg ttcaaaaatt atgtttcctc actaaaaaaa gaattttcag
      301 gttatgatct ttcaagattt cagaaagatc ttttggctgg gatcacagta gctgctgtag
      361 cattgccttt agccttggct tttggggtat caagcggagc tgatgcggcc gcaggtttga
      421 ttacggctat cattgccggt atcgttattg gcggtttatc aggcggtttt tatcaaatat
      481 caggtccgac aggtgcgatg gcagctatct tgatgtctat tgcggcaaca aagggaatgc
      541 aaggtgtatt gcttgctact tttttagcgg gtctattttt acttgcagca ggacttttcc
      601 gattaggaac actgacttct tttatcccag cacccgtaat tacggggttt acttcaggta
      661 ttgctatcat tattgcatta ggtcagatcg ataatttatt tggtgttcac tcggaaggta
      721 caaacgttat tgaaaaacta agtagttatc aaacgatagg atttccgata aacacagcct
      781 ctttgatcat gggagggcta gtcattttag ggatgtttat ctatccaaaa aaatgggcga
      841 aacgaatgcc tagttcgctg ctggctatca ttttggcaac agcagttatg ctattgttcc
      901 atttaccagt agcaactgtg ggtaagattc cacaaacatt gatcagtagt aatcgtttga
      961 atatgggaga ttttagcttt tctgccttgc aacaagtggc agttccagct attagcattg
     1021 ctttattagg aatgattgaa agcttgctat gtggtgcttc ggctggccga atggcaaata
     1081 agcccttaga tagcaatcaa gaattaatgg cgcaaggaat tggaaatctg ttgcttcctt
     1141 tctttggcgg gattcctgct actgcggcca tcgcccgtac aagcgtggcc atcaaatctg
     1201 gtgcacaaac tagattagct ggtatgatac atgcagttgt cttactgctc tccatgcttg
     1261 tttttgcgcc agtgatgtca cagattccaa tgccagcact tgcaggtgtt ttgattgtga
     1321 cggcttggcg tatgaatgaa tgggaaacga tcaaggagct atttactaat aaatattggt
     1381 cagcagttct attgtttatc cttacgatga tttgtacagt tatctttgat ttaagtattg
     1441 ctatcgtgat cggggtgatc ggcggctgtg tattttttgt tgtcaaaagc gctgctatca
     1501 cgatttcatt agaaacgatc gattggcaaa gaatggggct gccagaaacg gaaaagctga
     1561 ataactggac agtagtgtat attagtggtc cactgttttt tatgtcttca gaaaaactca
     1621 aaaataaatt ggaagaacta aatcatcacg atggtgtcat tttctccatg cgaggcgtac
     1681 caagtattga tttgacggca cgtgcagttt ttgatgaatt tcaagaaaaa gcagagcaaa
     1741 gaggacaaac gattatctat acttccttgc agccagaagt agaaaaacaa ctttctcact
     1801 tgtgggaaca tacattcacg catcaacatc aaacagttgc tcatgcgttg acagctttac
     1861 atgcgaaata tactgcaatt agccattaaa acaatgaaaa agaacagaca agactgtaaa
     1921 ctgaagattt tatggtcttg tctgttcttt ttaatattgc tgtaaaagtg aagctagtac
     1981 ttgccagtct tgatggataa caggtacaag ggagcgattg tagaaaatag ctgctggatg
     2041 aaaaagtgga agaattcgat agtttttttc tgaccattcg taggcatcct tttgttctgt
     2101 taattgtaag acaggccctg tatagagttg accatgattt ttggtaaccg tatattcttt
     2161 gccaagtaaa cgttgaagac caatgtttcc aagtgtcgca attaattttg gctggacctt
     2221 catcagttcg taatccaata ttggtgcatg cgcaaatact tcggaacgag taggtgtccg
     2281 atttggataa acagtttctg actgttgtgt ccgtttattg atccgatgag tcacgcgata
     2341 cgggcggctc cttacagcac tggtgatata cacctcttct cgtgttaagc cagttgaaga
     2401 aagtgcctgc atcaattctt ttccagcttg accgctaaaa ggaacatgcg tatcaatctc
     2461 tgttttccct ggggcttctc ccacgagcat cagtttagga tgaagaggtc cttgcccagc
     2521 gataaatcct tctacaggac ggccgttcat tcttaaagaa gcttcttgta tcagttcttt
     2581 tgggtattcc atttatgctc ctcctttttc ttttattata agaataaaag aaacacaatg
     2641 cgactatttt atttggtaga actgtgagga tagaaaagca gaagtattgc ttgaattttt
     2701 gttttacttt ttgattttaa atttagttga ctaacggttg ctgttctggt aagctagtac
     2761 tgttgtaatt gtggactcca cagctacaac cgctcagaac aagtaataag cacagcatat
     2821 gctgtcactt cggatggcga gtctaagtat atcagaagga ggtgctttat agcatgtacg
     2881 caattattaa aactggtggt aaacaagtaa aagttgaagt gggacaagca atctatgttg
     2941 aaaaacttga cgttgaagca ggagaaaaag ttgtttttga cgaagttatc ttagtaggcg
     3001 gagaatctac aaaagtaggc gctccaactg ttgcaggtgc aactgttgaa ggtactgttg
     3061 aaaaacacgg caaacaaaag aaagtcgtta ctttcaaata caaacctaaa aaacattcac
     3121 accgtaaaca aggtcatcgt caaccatata caaaagtggt tatcgacgcg atcaacgctt
     3181 aatagcgttt gagaaagaag gcctatatat gatcaaagga acatttaagc gaaatgacgc
     3241 aggtcagatc gtttcattca ctttgaccgg gcatgctgat gctggtcctt atggaagtga
     3301 tatcgtttgt gcgggtgttt ctgcgttagc gatcagcact gtcaacggga ttgcctcatt
     3361 agccggtttt gagccgattg ttgaaatgaa tgaagaagaa ggcggttatc tttataccga
     3421 agtcacttca ggaatgacac aagaacaaaa caatattgct gagattctat tagaaaatct
     3481 tttattagga ttacaatcga tagaagctga aaactcagaa tatatccaaa ttaaaacaat
     3541 taatgataaa taggaggtgc agaccatgtt gttatctatg aatctacaat tatttgccca
     3601 caaaaaaggt ggcggttcta catctaacgg ccgtgactca gaatcaaaac gtctaggtgc
     3661 taaacgtgct gatggacaaa cagttacagg tggatcaatt ctttaccgtc aacgcggtac
     3721 aaaaatctat ccaggtgtaa acgtgggtat cggtggagac gacactttat ttgcaaaagt
     3781 tgatggcgta gtacgtttcg aacgtaaagg ccgtaacaaa aaacaagtgt ctgtttatcc
     3841 agtagctcaa gaagcttaat tgaatcttat gattaaagag gttgtgacaa gacttttgtc
     3901 acagcctttt ttgtttataa aaataaaagt tgtagtggag gctacgaagt ttactttgtc
     3961 tttttacact taatattttt ttgccaattt ggaatcagga tgttttgata actatcgttt
     4021 tcaattgatg gaagctatat tgtttgctat cagtgaaata attatatggt aaacagtgtt
     4081 ctttttcttg tttccattat aaaaatcgta tcgggatttt tcgttctttt aaattgaaca
     4141 tttctatcta tccgcgtgtt atcaacaaaa caaaatcagg taaatagttg acgaaacaga
     4201 aataaataca tagtattatg ttttgaagcc taagtacaat agtctataga aaaggagcga
     4261 taccattggc aaaacgcatt gaccgaattt atatgtatgt caaagaaaaa accgctcatt
     4321 taacaaaggc agaatatgat caaggagtaa caacacagga aattgctgaa gtgttaggga
     4381 ttcagcgaac aaatagcagt aaagatctga atcagctagt gcgcgaagga aaactgataa
     4441 agacagacgg acgaccagta cgttacatat atcaaacact cgttacgcac gtaaagccag
     4501 ttacaaaaca tgtagtcagt tacaaagaaa cgccggtctc tgaagaaaaa aatacactcc
     4561 aaatagatac taaggatatc tttgcaaaaa tcattggagc taacggcagc atgaaaaatt
     4621 cagtggaaca ggcaaaagct gcgattttgt atccgccaag aggattaaat tgtttgatta
     4681 caggacctac cggatcagga aagacttatt ttgcacacgc gatgtatcat tttgcaaaag
     4741 cgcatcatgt aatagatgag gaaacggaac tgattgtttt caattgtgct gattatgcca
     4801 ataaccctga gcttttgatg agtcatttat ttgggtacgc aaaaggtgct tttacaggcg
     4861 cagatgaaga aaaaatgggg attatcgatc aagcggatgg cggtatgctg tttttggatg
     4921 agattcaccg gttgccgcct gaaggtcaag aaatgatctt ttactttatg gatcatggaa
     4981 cttacagtcg tcttggggaa acaacgaaat cccatgaagc aaatgtacgg atcgtaggtg
     5041 cgaccacaga agacccaggt tcttctctat tagaaacttt tgttcggcgt atcccaatca
     5101 atatcaaact tccggctttt gaaaaacgtc ctgcaaatga gaaagtcgat ttagtaaaaa
     5161 tcatgattgc ccacgaagca aaccgcacgc agcgaaagat ttctttgaca gaagatgtag
     5221 caaaagcatt gatcggcagt gtgacctatg gaaatatagg acaattgaag tcaaatatcc
     5281 agttagtatg tgcacgagga tttctaaatc atatgcaatc acccgaaatc tcgatcacaa
     5341 tcgatgattt gacagaaggg attcgtagcg gattgattca attagcaagc aaccgcagtg
     5401 caatggcaga gctttctaaa atattggagc caaagataac tgtagcacca aatgatacga
     5461 tcatgaaaat ccaatcggac tcctatgagt taccttataa tctatatgat atcatcggag
     5521 ataaagcagc tttactgaag tcggatggtt tggatcaaga agcaatcaat catttcattt
     5581 ctacagatat caacatccat ctgaagtctt tctataaaga ccatggtttt tcttttaatg
     5641 cagacagcaa attagcagaa tttgtagatc cgaaaatcat agaagtgacc aatcagattt
     5701 atgtaatggt caaaagatct ttaccatatg agtttcagca aaactttatt tatgctatga
     5761 gcttgcatat cagttctttt ttgaaaaaaa tcaacttagg agaagaacgg catacgaatg
     5821 ataacatccg tgagatggct tttgattttc cagatgaata tgctgttgcg aaagaagtgc
     5881 gtagttatat cgaaacgtat tttcaagtgg agattccaga aagtgaagac tattacttga
     5941 ccgttctact cgtctcgtta cgggcaaatc aagcaagcgg cagaatcggt gtagtcgttg
     6001 cagctcacgg aaacagcaca gcaagcagta tggtccaagt tgtccaacag ttgttagatg
     6061 cagagaacgt acgagcagta gacatgcctt tggatatgga tccgaaaaca gcattggacc
     6121 gaattgaacg caatgtacaa gaagtagatg aaggaagcgg ggtcatactc ttagtagata
     6181 tggggtccct tgcgtcgttt aattcgcaaa ttcaaagaga cactggaatt ccagtacgta
     6241 ctgttgatat ggtaacaact tctctagttt tggaaactgt acgaaaggtc tcagtattag
     6301 ggacagatct agatatgttg catgaatcat tgaaaaattt tcgaggttac gcagaaatat
     6361 cttctgtaga atctgttcag gaagcaaaac ctattcataa gaaaaaagct gttttggcta
     6421 tctgcgcatc tggcgaaggt acagcacaaa gaatcaaaga attgatcgaa cgagcggtaa
     6481 acaagcgtca agaaacagaa ttagaagttc tagctttgtc gattgtggaa gtgaaagaag
     6541 aactgccagt tatccaaaaa gaatatcaga tcatagctac tactgggatt actgatttga
     6601 atatccaagc cccatttatt cctttagaac gttttattga tcaaaatatc gaagtcattt
     6661 tggatcagtt gctgatggaa tcagaattgg aggaaactga attcatctcc ctagatgaag
     6721 agtcagctaa aaatacgtgt gttgagttta tctctgataa cttcatcttt attaatggta
     6781 gtaagttaat cgatccaatg tggcaatttt ctacacaaat ctctcagacg acgggaatcg
     6841 gcgatgaaga atatggattc aaaatcaacc tagtgatgca cacagcagga atgatcgaac
     6901 gtattattcg gaatgaacca ttgactgtgg aagaaaacga attgacaaat acaacgaatg
     6961 atcctcttta tagccaattg gcagcctcag ttgttctgtt ggaagatcag ataaaagtaa
     7021 aagtgccgat agaagaaatg tattatctgc tacgtctagt ccataatcag ttagataaaa
     7081 aagaatacac tgtgccatga atacacaaaa acgatacact tttatagtgt atcgtttttg
     7141 tgtattggtt aagaatcctt taaattcaat tagtgtatcg taaaaaagtt ggcacgcttt
     7201 ttgcttatag tagtgtgtgc taggaggtgg ttgttcacac agaaatgata ggggtttctg
     7261 tgaagtagtg aggaagaagg agttttgcag atggatattc gattggcacg tatagatgat
     7321 cgtttaatcc acgggcaagt cgcaacagtt tggtcgaagc aaacaggtgt tgaacgtatc
     7381 attgtgatca gcgatgtagt cgctcaagat aaattgagaa agtttctact aaaagaagct
     7441 gcaccgccgg ggatcacagc aaatgtaata accgtgcaga aaatgattca aatctatcat
     7501 agtggattat tatcacgagc aaaagtgatg cttttattca ccaatcccca agatgttgaa
     7561 gcggttgtcc gctgcggtgt acagttgaag tcgttgaata tcggcggtat gagttttact
     7621 gaaggcaaat caatgattac caattttgtt tcagtaaatc aacaagatat tgattccttt
     7681 cgttacttga acagtcaaga agtggagtta gagattcgaa aagtacctgc tgacaggaaa
     7741 atccttttga tggaactact agcgaaaatc aaaaaggtgt agcaagaatg gttgttggaa
     7801 aaggtcaaat aattctgttg taatggttag caattattaa tatttttgaa tttgttacaa
     7861 caaaagtaaa taggaggtat aaatatggta gggattatcc tagcaagtca tggacaattt
     7921 gctgaaggaa tcttacaatc cggttcaatg atctttggcg aacaagaaaa cgtcaaagcc
     7981 gtgattttga aaccaagtga aggtccagat gacttgagag ccaaattgga agaagcggtt
     8041 gcttcttttg ataatcaaga cgaagtgtta ttcttagttg atttatgggg cggaacaccg
     8101 ttcaatcaat caaacacatt attcgaagag cacaaagata aatgggcaat cgtcagcggt
     8161 ttgaatttac caatgttgat tgaagcctac gcatcacgct tttcaatgga atctgctcat
     8221 gaaattgcag cacacatcat tgaaacagca aaagacggtg taaaagtaaa accggaagaa
     8281 ttagaaccgg cagaagcacc gaaagcagca gttgaagacg cacaacctaa aggagcactt
     8341 ccagaaggaa ctgtggtcgg agacggaaaa atcaaatatg tattagcacg tgttgactca
     8401 cgtctgttac atggacaagt tgcgacagct tggacaaaag cagtgcaacc aaaccgtatt
     8461 atcgttgttt cagatgcagt atcaaaggat gatctgcgta agagactaat cgaacaagct
     8521 gctcctccag gtgtaaaagc aaatgttatt cctattagca aaatgatcga agtagcaaaa
     8581 gaccctcgtt ttggaaatac caaagcattg cttttatttg aaaatcctga agacgtattg
     8641 acagctgttg aaggtggcgt agacatcaaa gaattaaatg ttgggtcaat ggcgcattcg
     8701 gtcggtaaag tcgttgtaag taaagtattg tctatgggac aagaagacgt tgaagcgttt
     8761 gagaagcttg aacaaaaagg tgtgaaattc gatgtacgta aagtacctaa tgattcacgc
     8821 gataacatgg atgatatttt gaaaaaagca aaagctgaat tagcaaaagc gtaataaata
     8881 atattaaaat ttataggagg cttatcatgt ctattatttc aataatttta gtcgtattag
     8941 ttgcctttct agctggtatg gaaggtattc ttgatgaatt tcaatttcat caaccactag
     9001 tggcatgtac attgatcggt ttagttaccg gtaacctcga agctggtatc gtacttggcg
     9061 gtacgcttca gatgatcgct ttaggttggg caaatatcgg tgccgctgta gcaccagatg
     9121 ctgcattagc atcagtagca tcagcaatta ttttagtttt aggtggacaa ggtgttaaag
     9181 gtgttccttc tgcgatcgcg attgccgttc ctttggctgt tgcagggttg ttcttaacaa
     9241 tgatcgtacg tacaatcgct gttccaatcg ttcatttgat ggatgcggct gctgaagaag
     9301 gaaacatccg taaggttgaa atgtggcaca tcattgctgt atgtttacaa ggggttcgta
     9361 tcgcggtccc agcagcagcg ttgctattta tcccagcaca aactgtgcaa tcattccttg
     9421 aatcaatgcc agcatggtta acagatggta tggctatcgg tgggggaatg gtcgtagctg
     9481 taggttacgc tttggtaatc aacatgatgg ctacaaaaga agtatggcca ttcttcgtta
     9541 ttggtttcgt tgtagctgca atttctcaat taacattgat tgctctaggt gctttaggtg
     9601 ttgcattagc tcttatttac ttgaaccttt ctaaaatggg tggttcatca aataatggag
     9661 gcggaagcaa ctctggtgac cctttaggcg acattttgaa tgactattaa tcttgaagga
     9721 ggaaaataaa atggcagaag aaaaaatcaa attatcaaaa aaagatcgtt tagctgttgc
     9781 gtggcgctct accttcattc aaggttcatg gaactacgaa cgtatgcaaa acggtggctg
     9841 ggcattttct atgattccag caatcaaaaa attatacaaa acaaaagaag aacgttctgc
     9901 agcattaaaa cgtcacttgg aattctttaa tacacaccca tacattgctt ctccaatttt
     9961 aggggttaca ttggctctgg aagaagaacg tgcgaatggc gcgcctgttg atgatgtagc
    10021 gattcaaggg gttaaagtag gtatgatggg tcctttagct ggtgttggtg acccagtgtt
    10081 ctggtttaca gtacgtccaa tgctaggtgc actaggtgct tcattagcta tgggaggaaa
    10141 tatccttgga ccaatcatct tcttcttagg ttggaacatt attcgttggg cattcatgtg
    10201 gtatacacaa gaatttggct acaaagctgg ttctaaaatt actgatgacc tttctggcgg
    10261 attgttacaa gacgtaacaa aaggtgcttc aattttaggg atgttcgtat tagctgccct
    10321 tgtacaaaga tgggtatcaa tcaaattctt accagttgta tcaactgtaa aattagataa
    10381 aggtgcttac atcgaatggg ataaattgcc ttcaggtggt gaaggaatca agagtgcatt
    10441 tgaacaagtt aataatggct tagccctttc tcctgaaaag gtaacaacat tacaagacaa
    10501 cttagaccaa ttgattcctg gtttagctgc attaggatta acattgttat gtatgtggtt
    10561 gcttaagaaa aaggtttctc caatcgttat catccttggt ttattcgtag tcggtgttgt
    10621 tggacacgtc atcggacttt tataatccat aaaaatatag ctcgggcgtt gtcccgagct
    10681 ttttttatat agtatactgt tcataaacaa atatctggag ggagaaaatg gttcaatcaa
    10741 tcaatacaaa agtagacttg gtaattgatg ctacttcgtt tacgggtctg acagattacg
    10801 ggcaaatcat gataggtgat aatggttttg agttttacca tgcgcgagat agacgcaaat
    10861 atatccaaat tccatgggaa gaagtggatt atgtactagc atcagtcatg ctaaagggaa
    10921 aatggattcc tcgatatgcc attcaaacga aaaaaaatgg tacatacaca ttttcttcca
    10981 aagaacctaa aaaagtgctt cgtgcgatca gagaatatgt agatgaagat agaatggtac
    11041 aatcattgag tttctttgat gtagtgaaac gtggtattcg tagtatgttt aaacgcaagt
    11101 aaaaaataaa gaaaacttct aagaggaggt tttctttatt tttttacttt gcagaagaaa
    11161 atatgaaata gcaaaaccaa ttatttgtcc cctttaaatc ccatatgcta aaattgatta
    11221 gcaaagaagg aggttatatt atgagttggt tatggacatt gattgttgga gccgtgatcg
    11281 gagcaattgc aggtgcgatc accagtaaag gaaattcgat gggctgtgta acgaatatca
    11341 ttgctggact agtcggttca agtattggac aagcgatttt cggcgaatgg ggaccaaaag
    11401 ctgcaggtat ggcacttatt ccatctattt taggcgcagt gattttggta gcagttgtct
    11461 catttttctt tggaaaacga tcataagttt ttacaaaata gtaaactaaa aggagttgca
    11521 gcgagactgt gactcctttt tggctttatt tatttaaaat aaaaagaagt tttccggaaa
    11581 tttttattta actgtcgtgt acgaagaaaa gtttgctata atggaaaaaa gcgtgcaaag
    11641 aaagagggag tttcattatg atgctacgtg tagaaaaatt gcgcaaaaaa atgcaagaag
    11701 aaaatttgga ttcatttttg gttaccagtc cgtacaatct tcgttactta acgaacttta
    11761 caggtacgac aggattagct gttattactt tggaaaaagc attttttatt actgattttc
    11821 gttataccga gcaggcggca gcgcaggctc aagggtttga aattattaaa aatgtcggac
    11881 ctatttttga tgaggtagca aacttagtac gcaaagaaga actgcactca ttagcttttg
    11941 aggaaacaac agtctcattt ttagaatatt cggttttaga agaaatcatc gatgcggaat
    12001 tagtacctgt ttcacagatg atcgaagaac tcagagaagt gaaagatgaa gaagaaatcg
    12061 ctattatcga aaaagcatgt catattgcgg atatggcata tgatcacatc ctgaaaatga
    12121 tccaaccggg catgactgag attgaagtcg caaatcaact agacttttat atgcgttctc
    12181 ttggcgcttc aggtgtttct tttgatacga tcgttgctag cggattgcgt tcggctatgc
    12241 cgcatggtgt agcaagcaaa aaaatcatcg aacaaggtga cttgatcaca ttggactttg
    12301 gttgctatta cgaagggtac gtttccgata tgacacgtac attcgctatt ggtgatcctg
    12361 gggaaaaatt aaaagaaatt tatcaaatag tcttagatgc tcaattagca gttattgatg
    12421 ctgcaaaacc aggaatgact ggtgtccaat tagatgcagt tgctagagat tatatttcaa
    12481 aacatggtta cggggaagca tttggacatt ctacaggaca tgggatcggt ttagagatcc
    12541 acgaaggacc aaacgtttct tctcgtgcag aaaagcaatt tgtaccagga aatgtgatca
    12601 ctgatgaacc agggatctac ttaccaggaa tcggtggagt acgtattgag gatgacttgt
    12661 tgatcacaga agatggaaat cgtgtgttga ctcgttcacc aaaagaattg atcatcttat
    12721 agtttcatca atagaagaag taagcgtaaa aatcagtgtt cacagacttt gttatagaaa
    12781 atcactcgat ttaatgatac actatagaag aacttttaag ctgtaagaga taataaagtt
    12841 gggtgttttc tccaattcat agcagtttgc agctgatcgg cggcagtttt cttaccacgt
    12901 gatcactatg ccgcatcata gagaattcgg atgtatctcg tgcttaggca aagggaggaa
    12961 taaataaatg gcagatgaaa aaaatttgat tttaaacgca aaccaagatt taggagagat
    13021 cgtgatcgct cctgaagtca tcgaagtaat tatcgggatt gcagcttcta aagtagaagg
    13081 cgtatacgga atgcgcggaa cttttgccag caatgtcact gaattattag gacgtgcagc
    13141 tcacggaaaa ggtgtttact tagcaaacga ggaagatggc ttgaaagtag atctttactg
    13201 ttatctagaa tatggtgttt cagttcctaa agtagcaatg gaaatgcagg aacgggtcag
    13261 ccagcaagtc cttttcatga cggatatcga tttatctgaa gtcaatatcc atgtagtagc
    13321 tgttgttcct gaaaaattag aacaaccaac aatcgaagaa ctttttgaag acgaagagga
    13381 agagaatgaa taaagaatta tcaagacacg agattcggga aatggcattg caggctttat
    13441 ttccattaga cttcaatgca gacctaacaa aagaagatgc gattttcaac gcaatcgaat
    13501 tagaccaccg cgacatgatc aacgaagatg aatcagagtt tgttcctgtt tatcttgata
    13561 cgctagttgg cggtgtctgt gcaaaaaaag atgaattgga taaagtgatt gaaaaacatt
    13621 taaaaaacaa ttggcacatc aatcggatct caaaaatgga tttggtgatc ttgcgtatcg
    13681 cgatttttga aatgacttat gtgacagatg taccagcggc tgttgcttta aatgaagcaa
    13741 tcgagttggc aaaaacattt agtgatgacc gttcacgtaa attcgtcaac ggtgttttat
    13801 ccaatgtatt gaaagaatta gaagctgggg cgtaagtcct agcttttttc tatgaatcat
    13861 cataaacctg ttataaatcg ggaaaattct atggtagaat gaaaacacaa aaaatatctg
    13921 ggagtggtta gatggcagaa ttgattaacg gaaaagaatt agctgaaaaa atgcaggcag
    13981 agattgctga aaaaatccaa aaattgaaag ataatggtat ccatcccgga ttagtcgttt
    14041 tattggtagg ggaaaatcca gccagccaaa tctatgtaag aaataaagaa cgtgtagcaa
    14101 aagaaatcgg aatccattca ttggtagagc gttatcctga gacgatttct gaagacgaac
    14161 tacttgcaga aattgaaaaa tacaatcaag atcctagttt tcatggtata ttggtccaat
    14221 taccattgcc aaagcatata gatgaagaaa agatcctgtt agctatccgc cctgaaaaag
    14281 acgtagacgg ttttcatccg atgaatttag gcagactcct tgcgggtaaa ccggacaaga
    14341 ttccttgtac tccatacggc atcatgaaaa tgtttgaagc ttattctatc gatccggcgg
    14401 gtaaacgagc agtagttatc ggacgaagca atatcgtcgg taaaccgatg gctcagctac
    14461 tcttgatggc agatgcaact gttactgttg ctcattctaa aaccgaaaat ctggctgaac
    14521 tggcacaaga agcagatatt ttagtcgtag caataggacg tggacatttt gtcacaaaag
    14581 aattcgtaaa accaggtgct gtagtgatag atgtagggat gaatcgtgat gctaacggta
    14641 agctgatcgg tgatgtgaaa tttgatgaag tggagcctat tgcaggctgg atcactcctg
    14701 tgccaaaggg tgtagggcca atgactatca cgatgctgat gtatcaaacc gtaaaatctg
    14761 ccgaagcttt tggagcaggt gaatagatgg cacaagaata tttaactgtc actacattaa
    14821 cgaagtattt gaaaagaaaa tttgatgccg atccttattt agaacgcgta tatttaacag
    14881 gcgaaatatc gaatttccgt ttacgtgcca atgcccatca atatttcagc ttaaaagatg
    14941 accatgcaaa aatatctgct attatgttca aaggagcatt ccaaaaatta cgattccagc
    15001 caaaagaagg aatgaaagtc cttgtcgttg ggcgtatttc attatatgaa gcgggaggtt
    15061 cttatcaaat ctatgtagag cacatggagc ctgatggaat aggtgcttta tatcaagaat
    15121 tggaagaacg aaaagaaaag ctgatgaagg aaggcctttt tcaagggcct aaaaaaatgc
    15181 tgccacgttt cccaaagcga atcgcggtcc ttaccagtcc aagcggagcg gttatccgcg
    15241 atatcatcac gacggtgaaa agaagatatc caattgtcca attggttttg ttcccaacag
    15301 ttgtacaagg aaaccaagca gcggacaatg ttgtcaggaa tatccagaga gtagaagaga
    15361 tgggcaattt cgatacaatg atcatcggtc gtgggggtgg ttcgattgag gatttatggc
    15421 cgtttaatga agagcgggtt gctcgggcga tttatcaagc taatacgccg gtgatttcat
    15481 ctgtaggaca tgaaacagat accacgatcg ctgatttagt agcagatgtt cgggctgcca
    15541 ctccaacagc agctgcagaa ttagctgttc ctgttttgtc agaagagttg atgcgcattc
    15601 aagaacggca agctagattg cagcaggcgt ttgtacgcca gatccagcgc aaacaagaac
    15661 ggttcgagcg tgctcgacgt tcctatgtat ttcggcagcc tgaaagattg tatgaaggtc
    15721 agaccatcaa attagaccag ttgaaacagc gattatatca agctacacag caaatttatc
    15781 atgcgaaaga aaaacaaacg atcgcattaa gccaccaatt ggaacaagtg gctccgcttt
    15841 atcgagttaa agcagctcgc caagaaactg cttatctaga aaaacgctta gaagagaaaa
    15901 tccaacaata tatgaaacaa cagcagcagc gttttgctca agctgttcaa tctcttgatt
    15961 tgttgagtcc attgaaaatc atgggaagag gctacagcta tacgacgtta aatgatcaag
    16021 tagtaaaaag tgtcaccgag attcatcaag gggatttgct gacgatccat tatcaagatg
    16081 gaatcgttaa aggacaagta caagagatag acaaaggaga ataaaaatat ggaaaatcca
    16141 acttttgaag agtcactgca agaattagaa aaaattgtca tgcagttaga acaaggagac
    16201 gttccattag aatcagctct agattcgttt aaacgaggaa tggaattaag caaacattgc
    16261 caagatacat tagctaaagc agaagagacc ttgacgaaaa tgatgacaga atcaaatgaa
    16321 gaagttgatt ttgacggaaa cgaggaaagc tgagatgaga ttaactgact tttcagctat
    16381 tcatctacct gctgtagaaa aagaaatctt gtctttttta gatgagcata caacggatag
    16441 aggactctat gatgcgatga gttattcagt gaaagcaggc gggaaaagaa tacgtccctt
    16501 attactttta acggccgttg cttcctttga tgagcctatt gatgttcccg tctatcaagt
    16561 agcagctgct ttagaaatgg tgcatacgta ttccttgatc cacgatgatt tgcctgcgat
    16621 ggacaatgat gatcttcgta gaggaaaacc taccaatcat aaagtattcg gcgaagcatt
    16681 agctatattg gccggagatg gtttgcttac aggagcattc cagctaatca gtatggcaca
    16741 cttaggtaat tctcctaagc ttctgctgct tcagcaatta gctgtttgtg cgggatcaca
    16801 aggtatggtt gctgggcaag ctgcagatat cgagggcgaa tcgaaaaagc tttctttaga
    16861 agaattagca tttattcatg aaagaaagac agggcatttg attcgttatg ccttgctggc
    16921 tggtggcata ttagcgaagc aacctgaaga aatcttattg ctacttcaac gattagccga
    16981 acatttaggt ttagcttttc agataagaga tgatctactc gatgtgattg gtacaacgaa
    17041 aacattaggg aaaacagctg gtaaagatga acgtatggaa aaaaatactt acccgcgttt
    17101 attaggccta gaaaagacac gtgaggcgtt ggagattgag ctgttatccg ccaacaagat
    17161 catagacaaa ttagaagagg acgttttttc ttttgatggc gggctgatgc gccagatgat
    17221 caagcaattt gatgtggaga aatgagggaa agaatggaaa aagaacgcgt agatgtatta
    17281 gcggtcaaac aaggattatt tgaaacaaga gaacaagcca aacgttcagt gatggctggt
    17341 ttgatttaca acgaaaaaaa cgagcggttt gataaaccgg gagaaaaaat tccagtgagt
    17401 agtgaattaa gagtcaaagg taaaaaactt ccttatgttt ctcgaggggg attaaaatta
    17461 gaaaaagcac tcaaagcatt tgatctatct gttgaaggaa aaacattgtt agatattggt
    17521 gcatccactg gcggatttac tgatgcagct ttgcaaaatg gcgcaaaaat gagttatgct
    17581 ttagacgttg gctacaatca gctagcttgg aaaataagac aagatcctag agtcgtcgta
    17641 atggaaagag tgaattttcg ttatgcaaaa cccgaagact ttaccttagg aattccagaa
    17701 gttgctgtta tcgatgtttc ttttatttca ctaaaactga tgctgcctcc attacatgct
    17761 attttaaaag aagagggaga agtgattgct ttaatcaaac cccaattcga agcaggcaga
    17821 gaagctgtag ggaaaaacgg aattgttcgt gaccctcaga cccatcaaaa agttttagaa
    17881 gacattttgt catttgcggc acaaggtgga tatgatgtgc tggaattaag ttattctccg
    17941 atcacggggg gagaaggaaa tatcgagttc ttggctcact tgaggaaagt acccgaaagc
    18001 ggaacaatca attcagctat taatatggca gaagtggttt caaatgcaca cgaacagttt
    18061 gaccataaat aaattgtaac tgtgaaaatc aagtgagaag tatgcggatt cttctttatt
    18121 ataccaagga aataagctat cattgattaa gaagaatatg ctaaggagcg aagacgtaat
    18181 gagaaaaaaa gatcggcatc gtttgattac tcgattgttg agtgagcaag atatccgaaa
    18241 gcaggaagaa tttgttgaaa tcctgaaaag caaaggaata tctgttactc aagctacgat
    18301 ttccagagat ataaaggaac tgaaactgat caaggtaccg gcttcagatg gcggctaccg
    18361 ttatagcata ccagctgaaa caaacgaaga tgtaaatact aaattggaaa aactattaaa
    18421 agacgctttc gtggcagttg accaaatgga aaaatttgtt attttgaaaa cattgccggg
    18481 aaatgcttcg gcagcagcaa atttgattga taaacgttac aagaaagaat tattttcgat
    18541 tatcaatgat gacgataatg ttttgatgat tacacgaact gaagaccagg cagttttatt
    18601 gaagaaagac ttcctgcatt acctttaagt tgaggagcga atgtgatgct acaagaaatc
    18661 agtattacta actttgcgat tattccggaa ttgcggcttt cttttcacga aggaatgacc
    18721 gcgctgaccg gagagactgg tgcgggtaaa tccattatta tcgatgcttt aggtctgtta
    18781 gcaggaggaa gaggttcaag cgattatata cgtcaaggag cagaaaaatg tgtgcttgaa
    18841 gggttgtttg aactaccaaa acaagaagga ttctctgaac tgatggttga attagggatc
    18901 gaaacagatg aggacaatct gattgttcga cgggatatgt ccttaactgg aaaaaatgtt
    18961 tgtcgagtga atggtcatat tatcacatta gctaatctga gaagaatcgg cagttattta
    19021 gtagatatcc aaggacaaaa cgaacatcag gaactgctcc agcctgaatc acatcttgct
    19081 ttattggatc gttttggaga tgtagctttc caacagaaga aaaagagtta tcagcaagaa
    19141 tatatctctt atcgtgagct ggaaaaaagg gtacgaaaag tgcagcaaaa tgaaaaatcc
    19201 tatgttcaga gaatagatat gctccatttc cagcaagaag aaatagctgc tgctgatttg
    19261 caagtcggag aagaagagaa attaaaagaa gaaagagaaa aacttagcaa ttatcaaaaa
    19321 attgctgatg gattagctgc aggttacgga gcactgacga ataatgaaca aaacagtgta
    19381 gatggtgttg gcttggccgt ttcagaactg caagggatcg cccatctaga tgtggagtat
    19441 gaagcaattt atgaaaatat ccaaagtgct tactatttgc tgcaagatgc aatcggcgat
    19501 atgagtcgtc agattgattt attggagtta gatgagagtc gtttagaaga agttacgcaa
    19561 cgtttagaat taatccgaca gttgaaacgt aaatacggcg aatccattga gtcaatattg
    19621 gcttactatg acgaaatcac cgaagagcta gcttcttctg atttttcaga aggtcaattg
    19681 gacaaaatga aagaggaact agagcaaaaa gagcttctac ttcagcaaca agccgcagat
    19741 cttcatgaag cgcgtaaaaa aatcgcaaaa gaattggaaa aatccattct gcatgaattg
    19801 aaaagtctgt atatggaaaa cactgagttt gaggtacgtt tcttaaaaga agaaaataga
    19861 caattgaatc atgatggatt tgaccaaatc gaattttaca ttacgaccaa tccaggagag
    19921 ccactgaagc ctttggtgaa agtcgcttct ggtggcgaac tgtctcggat gttgcttgct
    19981 ttgaaaacta ttttttcttc tgagcaaggt gtgacaagta ttatttttga tgaagtggat
    20041 acaggtgtga gcggaagagt tgcacaggcc atcgcagata aaatttctca aatatcgaaa
    20101 tactctcaag ttttgtgcat cactcatctg cctcaagttg ctgcagtcgc cgactatcaa
    20161 tattatattg tgaaagcagt gattgatgga cgtacgcaaa cttctgtttc agagttgaaa
    20221 acaaaagaaa gagaagaaga aattgcacgt atgctggctg gaagtgagat tacaaaactc
    20281 acactcgaac atgcaaaaga actattgaaa ctcgcaaaaa ggacctgagc cacctcgcag
    20341 tggttcaggt ccttttcagc agctgatagc tatattttat ttttacatga cactcattac
    20401 agcagtcaat acaacagatc caatcgttaa aatcagcatt gtccaaatga caaattttgt
    20461 tactttagca aaagtacttg tttgtttttt ttcacgcatt gttttcaacc tcgtcattaa
    20521 ttagttgatt caagtgtaca atcttttttc catttccaca agagggcttt tttataaaaa
    20581 cttccctttt tttaaataat aggtagttac gatacaaata aataatgtta atagaactag
    20641 ccatataaag gcatcatccc tttcagcaat aggtaactta atgttcattc catagatact
    20701 gccgacgatt gtcggtatag ttaaaataat cgttaaagaa gttaaaattt tcataatatt
    20761 attcagatta ttcgatacga ttgctgaaaa agtctgattc atttgttcta ctagttttaa
    20821 ttgaatgcga gaagtagtca ttgcctgctc tgtttccacc aatacatcac gtaaatgatt
    20881 tttcaaagca tgattttctt ggaaaagttt attttgcgct agttctttca atgcatctaa
    20941 attagcagaa gtcgcttctc tgaaataaac gaggcttttt tggatatcca tgatttgata
    21001 aagttgtttg ttttcagtgg atacttgaag tttggcttcc aaagtatcgc attgagattg
    21061 aagcgtagct aaataactgt tgtaagagag tgtgacctgc caaatgaatt gaaagaagat
    21121 attgagatta ggcgaaatat cactttttgg aaaggtctgg ctaaagactt tctttaaaaa
    21181 cagtggttca tgattcgtaa ttgtgataag cttctttttg ggcgtcaaaa taatagagag
    21241 cgggtatgta tccacttgaa aataaccgct aggacttgtt ttagcaaaag gatattgtaa
    21301 caataataac gctggctttg tcaactttga ttgattgaat tcttctgtac gggaattttc
    21361 tgcatcgtcc aatatggaag tgatgtaatc tgacggaatt tcaaattgat gtgtcagctg
    21421 atgaatctct tcttgattag gacgttctac acaataccag atcgtttggt cagtttcctt
    21481 tccggtatta cgcagtttct cattatctaa tgtaaaatat ttgatcatga ttttcctcct
    21541 gttgcctgtt tcttttatta taacggatag ggaattggaa tacatggaat gtgtttgtaa
    21601 gatatagaat ataactgcat aaatttaaat aaaatgaaac agttgtgtaa agtatataca
    21661 gtatacttga agcaacgata aaaaaggagt ttacgttcat gggcacattc acaccactta
    21721 tcttgttttg gtatatttac ccggtaatcg ttttgtttgg ttgccaattt cttgtaaaat
    21781 tattttcgtt aaatcgtcgt tttaaaataa aagcccctga tttggcgatt ccttttttgt
    21841 ggggcggtat gcatgcattg tctagaaata cagggacaat ttctatctta ccgtttttct
    21901 ttatttctgt tttgctaatg ggaatttgta ttgctgtttt ccaagcatat tattatgaag
    21961 aaattattta tccgcgttat ttcaagatga cttggcgttt ggtttttctg ttgacaatcg
    22021 tcttgtacgc tgtcttgatc attgtgaaca tactttctta cctatgaaaa aaatagaaaa
    22081 gctgtaaaaa gtcttttaaa aggctttttg cagcttgatt tttaaaaaaa tgtttatgaa
    22141 taaaaaaagt tgttcccgga tttcccagaa aaggtggtag aaagtggggg attgtggtag
    22201 actaatttta gttagtggag gaatgggggg ctttcaatgt tgatgggtga attccaacat
    22261 aatattgatg caaaaggacg tttaatcgtg ccctcaaaac ttcgtgaaga acttggcgaa
    22321 aagtttgttc tgacacgcgg attggatggc tgtcttttcg gctatccgat gagcgaatgg
    22381 gaaaatctgg aagctaaact gaacgaaatg ccgttggcta aaaaagacgc acgtactttt
    22441 gtgcgattct tctattcggc tgccactgaa tgcgaacttg ataaacaagg acgaatcaat
    22501 atacccagca cgttaagaaa ttatgcagct ttgacaaaag gttgcgtagt catcggtgta
    22561 tctaaccgaa ttgaaatttg ggatgagaca cgatggcaag atttttctgc agcagcagaa
    22621 gagaactttg acgaaattgc tgaaagtatg attgatttcg gactataaga ataggaagag
    22681 gctgactaat gactgaaaca tttcaacact atacagtcat gttgaaagaa acggtagatg
    22741 gattgaacat caaaccggat ggcgtttatg tagactgtac actcggcggt gccggacata
    22801 gtgaatattt attaagccaa ctaagttcta aagggcatct gtatgcgttt gaccaagatc
    22861 aaaaagcaat cgatcacgcc aagctgcgtt tagcttctta tgtcgaacaa ggtatggtca
    22921 catttatcaa agcgaacttc agagaattag aagaacgctt gagtgagcat gtagataagg
    22981 tagacggcat tttgtatgat ctaggggttt cctctcctca gctagatgaa gcagaacgtg
    23041 gatttagtta tcatcaagat gcaccgttgg atatgcgcat ggatcagtca gctcctttat
    23101 ccgcttatca cgtaattaat gaatattcct accatgaatt agtaaagatt ttcttccgct
    23161 atggcgagga gaaattttcc aaacaaattg ctcgtgaaat cgaacgagta cgaaaaacaa
    23221 aaccaatcca aacaactggt gagcttgtcg aaatcattaa acaagtcatc ccagctcccg
    23281 caagaagaaa aggcgggcat cctgcaaaaa gaatttttca agctgtgcgg attgcagtaa
    23341 atgacgaact cggtgccgaa gaggcctctt tagaacaggc aatccggtta ttgaaagtca
    23401 atggaagaat cagcgtaatc acattccatt cattggaaga cagaattgtc aaaagtatgt
    23461 ttaaagaata tagtacggta caagatctac caccgggact tcctgtcata cctgacgaat
    23521 tccaaccaga actgaaagtc attactcgta aaccaatcct tccttcagag gaagagctaa
    23581 cagaaaacaa tcgatcacgc agtgcaaagt tacgtattgc agaaaaaata agaatgaaag
    23641 aggagtagaa tgatggcaga attaaagaaa atgcaagatt acgaatatga tttacctgaa
    23701 ttggtggatc agccggaaca acaaatgcct gattcttctt cgaatcaaga aatcattctt
    23761 ccacaatcac cggcaaaacg attaaagcag atttctgctt tggaaaaaat tgctgtggca
    23821 agcgtggcga ttgctattgt tgctttatgt gttttaacag ttatgctgcg cacaaatatc
    23881 agcggagctg aaaaagaaat cacgtcaata caaacagaaa tcaatgataa aaagcaagaa
    23941 aaaacgagtt tagaacaaga aaaaaacgaa ctttctcgta ctgaacggat taaacaaatc
    24001 gcagaagaaa aaggattgtc tattaatgac gacaatttaa ggaaagtgaa gtaaatgagt
    24061 cttaaaaata aaattcggcg gtttatggaa aagaaaaatc taaatccgat gaacaatcgc
    24121 aaaaaagtag gtatcatttt atttgctacg agtattggat tgttcttttt atttgccgtc
    24181 cgtttctcgt atatcgttat tggaggtcat gttgctggta cctcattagc tgaaaaaaca
    24241 aaacagttgt accaaggaag tgaagtagta aaagcaaaaa gaggaacgat ttatgatcgt
    24301 aacggtgtgg cgctcgcaga agatgcctcc tcttattcaa ttaaagcgat actctcaaaa
    24361 acatatacct ctggggataa aaaactgtat gttgaagaaa agaattttga taaaatagca
    24421 gaaatcctgc ataagaatct ttctatagac aaaaaagatg cactgaatat tttagaggac
    24481 ggcgcaaaaa aagagctgta tcaagttgaa tttggtagct atgggaaaaa tatctcccaa
    24541 gaaaccaagc aaaacatcga agcggacatg aaaaaagaag gtgtcgctgg tctttatttt
    24601 gtcgaccatc aggctcggat gtatccaaat ggcgtctttt cttctcattt tattggatac
    24661 gcagttccag acaaagatga gaatggctta gttggtaaac taggtttgga gtctgcttac
    24721 aatgatatct tgagcggtaa agatggtaag attatttatc aaaaagataa ttttcaaaat
    24781 cctctgccag gtacagtggc agaagaagaa aaagctgtcg acggacaaga tatctacacg
    24841 actttagaca gcagactaca aagttattta gaaacactga tggatcaagt aaatgaagaa
    24901 tatcagccag aagaattgac agcagtcctg atgaaagcaa aaaccggaga aatcttggca
    24961 atgggacaac gacctacttt caatcctgaa acaatggaag gattgactgg aaaagatgct
    25021 atctggcgta atttcttggt acaagatagt tatgaaccag gatcaaccat gaaagttttc
    25081 actactgcag ctgctatcga agaaggagaa ttcaacgaga atgaaacctt ccaatctgga
    25141 aaaatccaag tagaagatgc tacgatcaat gaccatgact ttggtgaaaa aggtgtgctg
    25201 accatgcgtc aagctctttc atggtcaagt aacgtcggga tggtcatact ggaacagcga
    25261 ttaggcggac gatggtataa ctatttacaa aaactgggat ttggacaaag tactcattct
    25321 ggattagatg atgaagtaaa cggtgcttta cctacattaa atattgtcga ccgagcaatg
    25381 agtgcttatg gacaagcggt aggtgtgacg aatttccaaa tgatgaaagc tttcacttct
    25441 attgcgaata acggtacaat gattcaacct cgatatatca gcaaagtggt ggatccccaa
    25501 actggagaag agcgaacaac tcaaacagaa gttctagggc aacctttttc aaaagaaaca
    25561 acagaaaaag tccgtgaata tatgagagat gttgtcgaaa gcgaaaacta cggtagtgct
    25621 tatggtgtat atagtgtacc tggatacaat gtatcggcaa aaaacggaac agcccaaatc
    25681 gcttctgata cagggggata tcaaacagga gatacagcct acctgtattc gattgtggag
    25741 atggttcctt ctgaagatcc agactatgtc ttgtatctaa caatgaaaca ccctaagaca
    25801 tatgatcgaa tggcattggc taaaatcgca aatccattga tgaaacgggc aatggatttt
    25861 aaagaaactg aagaagattc agatacagaa acaaaaacag aaaaagtatc cgtagccgat
    25921 tatcgtaatc ttgaagcgga cgttgcagca gctgatgctc aaaaaagtgg actgcaacct
    25981 gtagtgatcg gtaatggtaa aaaagtacaa aaacaatcta ctgccaacgg tgatcagttg
    26041 atctctggag aaaaactgat cctatatact ggtggtgata agctgatgcc tgacgtaaca
    26101 ggatggtcaa aagcagatat catgaagctg gggaaaatcc tcggtattga agttagtttt
    26161 gatggagatg gttattgtgt caaacaagaa cttgctccat atgaaaaaat cactgaagac
    26221 aaacttagtt ttactttaga agaataagaa gagaaatagg agagaaaaga gcatggaatg
    26281 gacacaagca ttgattccca ttgtcagcag ttgtgcaatg acaattgccg caatgccgct
    26341 atttattggt tattttcaaa tgaaaaagca aggtcaggcg attcgtgagg agggaccaaa
    26401 gtggcacaat agcaaagccg gaactcctac aatgggcggc ttagtgtttc tgatcggctc
    26461 tattttgaca ggaatttggg ttggagcttg gcaaaaacag ctaactccta ctcttttcat
    26521 cttgcttttt gtattagcgt tatacggagt aatcggcttt cttgatgatt ttataaaaat
    26581 attcaagaaa agaaacatgg gactgaactc gaagcagaaa ttgattggac agattctagg
    26641 gggaatcatc ttttatcttg tttaccgttc tgaaggttat ccaggtgtat tgaatttctt
    26701 cggactagat ctacctttag gaatcattta tggtattttc gctctcttct ggcttgttgg
    26761 attttcaaat gcagtaaatc tgacagatgg catcgatgga ttagttgctg gcttaggaag
    26821 tatctctttt gcagcttacg gtctaattgc ttggcatcag catcagtatg atgtattaat
    26881 catttgctta agtgttttag gaggtttact gggtttcttc gtctacaaca gaaaacctgc
    26941 caagattttt atgggagatg tcggttcatt agcgttgggc ggacttttag cggcaatttc
    27001 tataatgcta aaccaagaat ggacattgct tttgattggt ttgatctatg ttatggaaac
    27061 agcaagtgtg atgcttcaag ttacttcctt taagcttaca ggaaaacgaa tttttaaaat
    27121 gtctccaatt catcatcact ttgaaatgtg cggatggtcg gagtggaaga ttgatacagt
    27181 tttttggttg atcagcattg tgacttcgtt gatcacatta tggatcgttt ggtaggagat
    27241 gacgggaaat gaaaaaaata tcaacatatg aaaataagaa agtgcttgtc ttaggactcg
    27301 caaaaagcgg agtaagtgcg gccaaattat tacatgaatt gggcgcactt gtcaccgtta
    27361 atgatggaaa accttttgac gaaaatccgg aagcacaaga gctgctttct ttagggataa
    27421 aagtaataac aggtagtcat ccaatcgaat tgttggatga agaattttca ttgatggtca
    27481 aaaatccagg gattccctac agtcatcctt tagtagcaaa agcccaagaa atgggtattc
    27541 cggtaattac ggaagttgag ctagcttacg aagtagcgga atgcccaatt attgggatta
    27601 caggaacgaa cggaaaaaca acgacaacaa cgatgacagg actactactg aatgcaggag
    27661 cagatcaagg aattgcacgt ttagcgggta atattgggta tccagcaagt ggtgtagcgc
    27721 aagaagcaaa atcagaagat aaaattgtga tggaattgtc tagcttccaa ctcatgggaa
    27781 tcacagattt ccgccctcat atagcggtga ttaccaatat ctatgaagca catattgatt
    27841 atcatggtac aagaaaagaa tatgtcaaag ctaaatggaa ccttcaaaaa aacatgacag
    27901 aaaaagatta tctgatcctt aactggaatc agtcagaact acaagaatta gcgcaaagaa
    27961 caaaagcacg cgtactgcct ttctcgacaa aagaagtgtt ggaagacggt gtgtatgctg
    28021 acgattactc aatatactat aaaaaagaaa aaataatgga aatatctgaa cttggcgtac
    28081 caggtaagca taatgtagaa aatgcactag ctgctatttc tgtagcaaaa ttgtatggta
    28141 tatcaaatga agctatacga gaaacgttgc attttttcca tggcgtacca catcgtacgc
    28201 aatatgtagg agaaatccaa ggcagaaaat tttataatga ttcaaaagca accaatattt
    28261 tagcgactaa aatggctcta agtggatttg aaacgtctaa ggtcgtgtta ttggctggcg
    28321 gattagacag aggaaataca tttgatgaat taattccttc attaaaagga atcaaagcaa
    28381 tggtcgtatt tggccaaaca aaagaaaagt tgatggatgc tggaaaaaaa gcaggcatcg
    28441 agacaatagt aacagccgat tctgtagaac aagcagttcc tttagcatta gaaaacagta
    28501 cagatggtga tgtagtttta ttatcaccag cgaatgccag ctgggatcaa tacccaaatt
    28561 ttgaaactag aggaaacaga ttcatggaag ctgtgaaccg attaaaatag aaaaagtaga
    28621 agcgagcgat aaaaaatgaa aattttggtt acaggcggag gaacaggggg acatatttat
    28681 ccagctcttg cctttgtcaa ttatgtaaag actcaagaac cgaatgctga attcatgtat
    28741 gtgggagcaa aacgaggtct agaaaacaaa atcgtccctg atacaggaat gcctttccat
    28801 accttggaga ttcaaggatt taaacgaaaa atttccatgc ataacatcaa gacggtccga
    28861 ttgtttctta agagtatccg tcaagcaaaa aaaatcctca gtgattttca accagatatc
    28921 gttatcggta ctggaggata tgtttccgga gcggtagtgt acgctgcagc caaaatgggt
    28981 attccaacag ttatccatga acaaaacagt gtaccaggtg ttaccaataa atttttaagc
    29041 cgatatgtgg atcggatcgc tctttcattt aaagatgcgg cttccttttt tcctgaaaac
    29101 aaagcagttc ttgtaggtaa tcctcgagca caagaagtag cagatactaa aaaatcagat
    29161 attttgaaaa catttgattt ggatccagaa aagaaaacag tcttgatttt tggcggtagt
    29221 caaggagcac ttaaaatcaa tcaagcagta tcagtttttt taagtgaata tattccttct
    29281 tcttaccaag ttttgtatgc ttcaggagat agatattatg aagagatcct aaataaaata
    29341 aatcctgaaa tacaaaatat tagtgtccag ccttatatca aaaatatggc agaggtcatg
    29401 gcttccagtg atctgctggt ggggcgtgct ggtgcaactt cgatcgcaga attcacagct
    29461 ctggggttac cggcagtgtt ggttcccagc ccatatgtta cgaatgatca tcagactaaa
    29521 aatgcaatga gtcttgttca cgcaggagca gcaaaaatga ttgcagataa tgaactaaca
    29581 ggtgaatcac taagtcagac agtcaacgaa atcatgggtg atgaagaatt gcaaaagcaa
    29641 atgtgccgag cttctaaaga acaaggaatc cctgatgctt caaaacgtct ttatgactta
    29701 gtaaaacaaa taatcaaaaa ataaaggcgg tggtcttagt gagtaagaaa aaagaaccaa
    29761 aaaaattgtc tcatccgtct tctgatgaaa acaatttgac tccttggcaa aaggctaacc
    29821 gaaaatatct ggcagaacaa gagatgaaaa accaagagcc aaaaaaagaa gacataacat
    29881 tagatacacc cctttcagat tctgaagaag aaaaaacggt tcaagaggaa cagcaagaag
    29941 aaagccagtc cgagaaaaaa atcgaatttt ttgaagagat caagcaaaag aaaaacggtg
    30001 gaccatataa cggttctttt ttgaaccggc tgcccaattt aaaaagtcaa cggcacaaag
    30061 tactatttcg tcggttagct ttgatcatca ctgtattggg tataccgctg atttttttga
    30121 tctattatgt ttctccttat agcaaattac aagcagttgc agtatcaggc aataagacag
    30181 tcaacagcca agaaatcatc tctgatacaa aattgtcatt aggagaaaat gtttggggac
    30241 agtattttca tcgatctact tatatcgatc gccttaaaaa agcacagcca agaatcgaaa
    30301 cagcaaatat acatttcaaa gggatgaatg aattcgattt ggatgtaaca gaatataaag
    30361 agattgcttt gatcgcaaaa aacaatcaat actacccagt gattgaaaat ggaacagtat
    30421 tagatgaaaa agtagcaaat cctacaaaaa atctccctat cttggaagag ttcaaagact
    30481 ctgcaaaaat caaagaatta acaaagcaat acaatcagtt atcatcagaa ttacaaaaag
    30541 caatttctga aatcaagtac acgccaagag caagcaataa aaatctgatc caattgaata
    30601 tgaatgacgg caatcaagtt attgtaaata tcaataatct tgcgaaccaa atgaaatatt
    30661 attcgcaagt agccaaagat atggatgaaa aaggtgtgat cgacatggaa gttggtatct
    30721 tctcgcatcc ttacggtagc actcaagaaa cggaagaatc gggccaatcg gaagaaacaa
    30781 ctgaaagtga agaaaatgcg gatcaaacaa acgaatcaag tgaagaggca gaaacgcaaa
    30841 aagcacaaac acctggatcg tctagcacaa acggatcatc tgattcaaat gattcaagtg
    30901 aaacaagtca aatagaagat aatccaagta caactccgtc aagtttaaat gaaaattgat
    30961 aaaacaactt taaaaagtaa gaatgtactt tctaaaaacc agtaatttat ggtagaattg
    31021 gaagcagtga atatatctat taataagtat attttaaaac ggacgctagt tgttcgcttg
    31081 tatatggaat agtaagtagg agggaatccc attcatggca aaaacaggaa tgtatgtagg
    31141 ccttgatatt ggaacgacat ctgttaaagt tgtcgtggct gaatatatcg acagccaaat
    31201 gaatattatt ggagtaggaa acgcaaaatc agaagggatt aaccgaggca ttatcgttga
    31261 tatcgataaa acggtccaag cgatacaacg agcagtacga caagcagaag aaaaagcagg
    31321 tatccagatc aaaggcgtaa gtgtcggttt gccagcaaac atgcttgaag tagaaaattg
    31381 ccagggaatg attgcagtta acggagattc aaaggaaatc accgatgaag atgtcagaaa
    31441 tgtggcttca gctgcccttg ttcgttcaat acctcctgaa cgtcaaatcg tgtcaatatt
    31501 gccacaagat tttactgtcg atgggttcga gggaatcaaa gatcctcgtg gaatgatcgg
    31561 cgtaagatta gaaatgtacg gtctgttgtt tacaggacct aaaacgatca tccataacat
    31621 acgtaaatgc gtggaaaatg ccggattaat cgtcaacgaa atggtgatta ctcctttagc
    31681 gctgactgaa tcaattcttt ctgacggaga aaaagatttt ggtacgatcg tcattgatat
    31741 gggtggcggc caaacaacaa cggctgttat gcatgataag caattgaagt ttaccaatct
    31801 tgaccaagaa ggtggcgaat ttgtcactaa agatatctct attgtattaa atacgtcatt
    31861 taacaatgca gaagcgttga aaataaacta cggggatgca tacccagaac ggacatcacc
    31921 agatgaagaa tttccagtag atgtgattgg tcagtcagaa ccagtgaaag tcgatgaacg
    31981 ctacttgtca gaaattattt cagctcgaat ggaacagatt ttcaacaaag caaaagatgc
    32041 attggaccaa atcgaagcat tagagttgcc aggtggcgtc gtattgacag gcggagctgc
    32101 tagtcttcct ggtgttgtgg acttggcaca agaaatcttc ggcgtaaatg taaaattgta
    32161 tgttccaaat cacatggggc tgagaaaccc ggtatttaca aatgtaataa gtatcgtgga
    32221 ttattcagca aacttgagtg aagtgtatca attggcaaaa attgctgtta cgggtgaaac
    32281 ctcagcagct cgtcaaatgg tagttgaaca agaaacaaca aacacatatg agagctatga
    32341 agcacctgaa gaaacaatct acgatgagcc ggaaccaaaa gagtccggag aaaatgtaaa
    32401 aaataaaatc aagggtttct ttacaaatat ttttgattaa ttcatcctgg gaggaataag
    32461 atatggaatt ctcaattgat aataatatca atgacggcgc agtaatcaaa gtcatcggag
    32521 tcggtggtgg cggtggaaat gctgtaaacc gaatgattga agaaaacgtt aaaggtgttg
    32581 aattcattac agccaacaca gacgtgcaag cattaaaaaa ttcaaaagct gaaacagtta
    32641 tccaattagg ccccaaatat actcgtggtt taggtgctgg ttctcagcca gaagttggac
    32701 aaaaagcggc agaagaaagt gaacaatcct tacgcgaagc tttagacggt gcagatatga
    32761 ttttcatcac tgcaggaatg ggtggaggaa ccggtacagg tgctgcacca atcgttgcag
    32821 gtatcgcgaa agaattgggc gcattgactg ttggtgtcgt gacacgtcca ttcacttttg
    32881 aaggaccgaa acgtggccgt tttgctgcag aaggtatcgc tcgtttgaaa gaaaatgtgg
    32941 atacattatt aattatctca aataaccgtt tattagaagt tgtagataaa aagacaccga
    33001 tgcttgaagc tttccgtgaa gcagataatg ttttacgaca aggtgtacaa ggtatctctg
    33061 atttgatcac agcgccaggt tacgttaacc ttgactttgc tgacgtgaaa acagtgatgg
    33121 aaaaccaagg tacagcattg atggggatcg gtgtggctag tggagaagat cgcgtagtag
    33181 aagcaacgaa aaaagcaatc tcctctccat tgcttgaaac atctattgat ggagctgaac
    33241 aagtacttct taacatcaca ggcggtttgg atatgacatt gttcgaagca caagatgctt
    33301 cagatatcgt agcaaatgca gcaactggcg atgtaaatat cattctaggt acatctatca
    33361 atgaagaaat gggcgatgaa atccgtgtga cggtcatcgc aacaggtatc gatgaatcaa
    33421 aaaaggaacg caagtctagc cgaccagcta gacaagcaca aatgcaatca cctgctcaaa
    33481 agactgtatt agatatggat caagctaaac caacttcttc agaagaagaa aacagttttg
    33541 gcgattggga tatcagacgt gaacaaaatg ttcgtcctag agttgatgat tccaattttg
    33601 acaatatcga aaaaaaagaa tttgatacat tcaaccgcga agaaacaaaa tctcatggcg
    33661 atgatgaatt aaatacgcca ccgtttttcc gtcgtaaaaa ataaaggaga ggaagagcac
    33721 atgattgctg acaacttgca gaaaatcaag caagaaattc aggattcgtg tgctcttgtt
    33781 catcgtgatc cgaaagaagt tacattagta gctgtaacaa aatctgttga atccgctcaa
    33841 gctgaagaat tggttagagc tggcgttatg aattgtgcag aaaaccgagt agataaatta
    33901 ttagaaaaga aacaagattt atttgatttt cctgaaataa agtggcattt aattggtaat
    33961 ttacagcgca gaaaggtaaa attgattgta aatgaaattg attactttca tgcgttggac
    34021 agtttaagat tagcagaaga aatccagaag cggttagaga aagaattatc ctgttttatt
    34081 gaagtaaatg tttctggtga ggaatcaaaa catggaattt caccagatga gcttttaccg
    34141 tttgtggaat ctttagctgc ctgcacaaaa atcaaggttg ttgggttgat gactatggcg
    34201 cctaaagaag cttctgatga gcaaatccat aaagtgtttg gtacgctgaa agaattgcgt
    34261 gatcttgttc aagcaaaagg ttatgcccat gcaccatgta gtgaattaag tatggggatg
    34321 agtcaagact ttcaaattgc gattgaagaa ggtgcaacat ttatccgtgt aggaacagcg
    34381 ctattcaaag acgaataaag gaggaacaaa aatgccgctt ttaaacagag gagcaattgc
    34441 taattttttc ggattagctg atgaggaaga gtacgaatac caagattatc ctgaaacaac
    34501 aaaaaaggct gtacagccaa ctaaaaaaaa tacaacacag aatcaatcaa ctcattctgt
    34561 atctgctgcg aatactcatg aaaaacaaaa aacggcgact tcacgtcctg taacgcatga
    34621 aagtattcag ccggctgaaa agaaaaaagt aattgaacta catcaatcac ctacaaaaac
    34681 tgtagcgaaa gaaagaacag cttcaagaac ctctacacaa cctaaagcaa atcttgctgg
    34741 aaaaatcact gtaatggaac caagagctta ttctgaagct atggcgattg ctaaacgaat
    34801 cattgctgga gaagcaacct tagtgaattt tcatttagta gaagaaaaac aagcgagaag
    34861 aatcgttgat tttctaacag gaacagttta tgcactagat ggagatatca aacgtgttgg
    34921 agatgagatt tttctttgta caccgactgg gatggagatt gacagttcaa ccgctcaatc
    34981 ttttgcagat gcgaatttgt ttgatctgta agaggaggaa ctacgattta tactattgca
    35041 ctattacttc atttacttca acgggcagcc tatttctata gcattatctt gatggtatat
    35101 gcattgctct cttggtttcc aggcgggtat caatcagggc taggacgatt attagctaag
    35161 atctgcgaac cgtatatcag tttgttcgat cgtctgcctt tgaggattgg acccttggat
    35221 ttcacaattt taggggcaat cctgatcttg aacttagccg tacaaggttt agcacagatc
    35281 gttatatgga taactgtcca tttagtttat tgattgaagc aacaaaatct ttattctcag
    35341 aatagatagg aggggaaaat tttggatgca aatgtatacc agcactttcg ggcagatgaa
    35401 cgtacattta tcgattcggt aggcgattgg attgaacagg ttcaaggaca atatgcaccc
    35461 tatctgaccg aatttcttga cccaagacaa tcctatattt tagaaacact gattcgacaa
    35521 agcagtgatt tgcgtttcat gttttatggc ggatatgaac aggcagaaag aaaaagatgt
    35581 ttgattttcc ccgattatta cgaacctgta gaggaagatt ttgaaacagc attgtatgaa
    35641 atccattatc ctagcaaatt tgccagccta agtcatggga aaatccttgg aactttagtg
    35701 ggaacgggga tcaaacgctc ttactttggc gatatcatct cggacggtga tcgctggcaa
    35761 gtatttattg caaatgaggt ggatcatttt gttgctgcac aagtaacaaa gattggaaag
    35821 gttgctgtac gcttggaacc aatcaaatac acagaaatga ttatgccgaa agatagctgg
    35881 acacaagaaa gaggaacagt tagttccatg cgactagaca gtgtgatctc tgagatttat
    35941 aatatttcca gacagcggtc caagcagctg atcgaagctg gtaaagtaaa agtgaactgg
    36001 acagaaaata caagacctga ttttgtactt gaactattag atattatttc agtcagaggt
    36061 tatggcagag tccagatcca agaacttgag gggaagacga aaaaagaaaa gtacagggta
    36121 ctgttcggtg tattacgtaa ataaaataaa aaggtggttt actcatggct ttaactccat
    36181 tagatatcca aaataagaca ttcccgacaa aaatgcgggg atataatcaa gacgaagtag
    36241 atgatttctt agatttagtt gttcgtgact atgaggaatt gactcaaaga aatcgagaat
    36301 tagaaaaagc agtcaaacat tctgaagaaa aacttgagta cttcaatgaa ttaaaagatg
    36361 ctttgaacca atcaatcatc gtagctcaag atacggctga taaagtaaaa acaagtgcaa
    36421 gcaaggaatc cgaagtgatc gttacttctg cacaaaacaa agcagatgag ttagttgcga
    36481 acgctgaaaa acgtgcacat caattaacaa ctgatgcaga agaaaaagca agaaaaatct
    36541 tgacagatgc aacagaaaaa gcaagacagc ttgcgacaga aactgaagat cttaagaaaa
    36601 aaacacgagt attccatcaa cgtatcagct tgatgcttga gtctcaatta gaacaagtga
    36661 aaagtccgga atgggatgaa attttacaac cattctccag ctacgtgact gacagccatg
    36721 aagtgatcaa agaagtttta gcaaaacagc ttgacaatga aaatgatact gatgtaaact
    36781 cagacacagg agtcatagaa gcacctatga cggcatttga tacacaggct attgatatct
    36841 cgatcgtgcc tggtgaaaat gaaaacgagt agaacagaaa agcactttgt tattcttaaa
    36901 gcgagccgat gagagtggaa gttcggtaag acactgcatg gtgaaagatc ctctacttga
    36961 gacctgcttt tccgaaatta gagtaagaag cagcggctga cctcgttatt ggtcacagag
    37021 agtttgcact agatagatgc aactaaattt gggtggtacc acggcatgtt cgtcccttgc
    37081 ggctgaacat gcttttttgt gtccggaaaa caaataaagg agtgtatggt tgaatgaaaa
    37141 tgaaagaaac cttgcaatta ggaaaaacaa aattccctat gcgtggtaat ttgccaaacc
    37201 gagaaggcga gtggcaaaaa gaatgggaag aaaatcgaat ctaccaaaaa agacaagagt
    37261 tgaacgaagg aaaacctagc tttgttcttc atgatggacc tccatatgcc aatggtaata
    37321 tccatttagg acattcattg aataaaatca gtaaagacat tatcattcgt tcaaaatcaa
    37381 tgtctggcta tcgtgcacca tatgttccag gatgggatac acatggattg ccgattgaac
    37441 aagtattggc taataaaggc gtgaaacgta aagaaatgtc catggcagaa tacttggaaa
    37501 aatgtcgtga atatgcactt tcccaagtag ataagcaacg agcagatttc aaacgcctag
    37561 gagttgcagg agattgggaa catccttatg ttacattaga tccttcttat gaagctgcac
    37621 aagtacgcgt atttggtaaa atggctgaaa aaggctatat ttacaaagga ttgaaaccaa
    37681 tttactggtc tccgtcaagt gagtcttctt tagcggaagc tgaaattgaa tataaagatg
    37741 taaaatctcc atctattttt gtagctttca atgtcaaaga cggcaaagat attcttgatg
    37801 aagatacagc ttttgtcatt tggacgacaa caccatggac attgccagca aaccaaggta
    37861 ttgctgtaaa tccaaaattt acgtatgtat tggtggaagc cgatggccgt aagtttgtcg
    37921 tagcgaaaga tttgctagaa acagtccaat cagaaatcgg ttgggaaaat gtaacagttc
    37981 taaaagaatt tgctggacaa gaaatggaat atatgactgc aagtcatcca ttctatgaac
    38041 gtgaatcact tgtgatttta ggcgatcatg ttactttaga cgctggtact ggattagtcc
    38101 atactgctcc aggacatggg gaagatgact atatcgctgg tagcaaatat aaactatctg
    38161 tagtgtcacc tgtagactca aaaggattgt ttacagaaga agcacctgga tttgaaggta
    38221 tcttttatga caaagcaaat cctatgatca ctgatctatt aaaagaaaaa ggagctttgc
    38281 tgaagctaga tttctttact catagttatc ctcacgattg gcggacgaaa aagcctgtga
    38341 tctatcgggc aactccacaa tggtttgcat ctatcgataa attccgtcag aatattttgg
    38401 atgaagtaga aaaagtagag tgggttatcc catggggtaa aacacgtttg tataatatga
    38461 tccgtgaccg cggcgattgg gtaatttccc gtcagcgtgc atggggagta ccacttccga
    38521 ttttctatgc tgaaaatgga gaagcaatca tcacaccaga aacaatcgaa catgtggcaa
    38581 aattatttgc tgaacatgga tcaattatct ggtttgaaaa agaagcgaaa gaacttcttc
    38641 ctgaaggatt tacccaccca ggttctccta atggcgaatt tacaaaagaa aaagatatca
    38701 tggatgtctg gtttgattct ggttcttctc atgaagctgt tttacgtcag cgaccagaat
    38761 tatcattccc agcagatatg tatttagaag gatcagacca atatcgtggc tggttcaact
    38821 caagtatcac gactagtgtg gctatcaatg gtgtagcacc atacaaatca gttatttctc
    38881 aaggatttgc tttagatggt gaaggccgta agatgagtaa atctttagga aacatcattg
    38941 caccagaaaa agtgatcaaa caatttggtg cagatatcct tcgcttatgg gtatctagtg
    39001 tagattatga agcagatgtt cgtgtttcta tggatatctt aagtcaagtg gcagaagtat
    39061 atcgtaaaat cagaaatacg atgcgtttct tattagcgaa tacagaagat ttcgatccta
    39121 aaaaagatac tgtttcgtac aatgatttac gttctgtaga caaatatatg accgttcgat
    39181 tgaaccaagt aatcaaagaa atccgagaag aagggtacga caaatataac ttcatgcata
    39241 tttaccgtac agttatgaac ttcttgacgg ttgatctatc gtcattctac ctggattttg
    39301 ctaaagatgt agtttatatt gaagcagaag atgattatca acgtcgttgc atgcaaactg
    39361 ttttctatca aacagctgta gctttgacaa aattattgac tcctatcatt ccgcatacag
    39421 cagaagaaat ctggtcattc ctgaaagaag aagaggagta cgttcagtta agcgagtttc
    39481 ctggttatga agagtttgca aaccaagaag aattgatgga tacctggact gctttcatgg
    39541 acttccgtga taatgtctta aaagcattgg aagaagcaag aaacagcaaa ttgattggta
    39601 aatctttgga agcaaaacta actgtttatc caaaacaaca agttcgtgaa atgttaaaag
    39661 ctttggatgc agatattgca caattattga tcgtttcacc agactacttt gaagttatgg
    39721 cagcagatga acaagtgcca gataacgcta tggtatttga tgatgttgct attacagtgg
    39781 aaaaagcaga cggggaaact tgcgatcgct gtcgccaagt tcgtaaagat gtaggagtag
    39841 atgaaaaact tcctcatcta tgcggtcgtt gtgcaaaaat cgttgaagac ttctatcctg
    39901 aagctgtagc tgaaggattc gaagaaaagt aaaagggaaa agggggtgtg acatgttctt
    39961 tgtcacaccc ccttttgtta tcaatcgttt aattttccac gttcacgata ccagttatct
    40021 ggttgccaga tccattcgaa tccgtctttt tcaagtagtt cgaaagctgc tttaggtccc
    40081 attgatccag ctttgtagtt gggaaaatcc ggttgtgttt ggtcccaagc ttttcgaatg
    40141 acatccacga tatgccaaga ttgagcgact tcttcccaat gagagaagtt cgttccatca
    40201 ccattcaacg catctaatag taatttttcg taagcttctg ggctattttc aaccatttct
    40261 gcactatttc taaattccaa tttgattggt tccgtttcaa atccttgccc tacttctttc
    40321 ccattcaagg ataaagaaaa tccttcagtt ggctgaatat agatcgttag aacattaggt
    40381 ggaagtgtcg tatcgtcaca tggttcatcg acagatgttt tgaatacatt aacaggaact
    40441 tgtttgaata cgatgttgat ccgagtccct ttttcagtca aacgtttgcc tgtacgtaca
    40501 tagaaaggaa cacctgacca acggaagtta tcgatagtga atttgcctgc gacgaatgtt
    40561 tcagtctctg attgagcatc cactttgtct tcttcgcgat agcccttgaa ttgtacacca
    40621 tccaattcac cttgagcata ttgcccgcga acgaagtttt ctaacacttc ttcttcgtta
    40681 taaatgcgga tagctttcaa tgctttgatc ttttctgtac ggatttcttt ttcggaaaat
    40741 gctactggcg gttccattgc aagtaatgct acaacctgta aaatatgatt ttgcaccatg
    40801 tctttaagtg cgccactatg atcgtagtag cccccgcgat cttctacacc gaggttttcg
    40861 ccaaaagtaa tttggatatt gtcgatatag cgattgttcc attgagcctc aaaaatatta
    40921 ttggcaaaac ggatagctga gatgttttgg atcatttctt ttcctaaata atgatcaatc
    40981 ctgaaaatat cttgttctgg gaatacttct ctgatttgct ggttcaattc aaaagcagac
    41041 tggtaatcag aaccgaacgg tttttctatg atcaaacgat tgaagccatt ttttgtcata
    41101 atattttgtg atttcaaatg agaaacgatc gttccaaaaa attgaggtgc catagccaaa
    41161 taatagacac ggttaccgtt taattgatag tgtccatcta atcggtcaga caattctttt
    41221 aatgtctgat aatgcgttga atcatttaca tcatgggatt gatagtaaaa atggcttgca
    41281 aaggtagttg cttcttcatc tgaaggattc aatgcacgga ttgtttcttt gacaacttca
    41341 cggtagtgct catcgctcca gggacggcga gcggttccga ttacagcaaa atgctcgcct
    41401 aaatttcctt ttctgtaaag gcggaacaaa gacgggtaca atttacgctg ggctaggtca
    41461 cctgtaccgc cgaatatcgt aaacaataca tttttttcat tagacatgtg gatacactcc
    41521 tttatatttt atcgacaaaa atcgtgctgg ccgccttaaa gctgacgggg attttctttc
    41581 tatcattttg gatagtgatc ggtccttcat aagctgcgat gtcaacgatc tcatattctt
    41641 cgttgatatt caggtcaata gtgactaagt aatccaacaa ttctttttca tcaagcactc
    41701 gtgcgatacg cacttttgta ccgacaggat aggactccaa tgtttgtcgg cgttcttcat
    41761 ggacaacttc ctctttttta ggaatcgatc ctccatgagg acaatgttca ggatgattca
    41821 aatagttttc taaatgatct gcaagatgtt ccgaagtcac atgttccaat acttcagcat
    41881 catcatggac ttcattccag ctatagtcta aatgttcaac taaaaaaact tcccataagc
    41941 gatgtttgcg aatcagactg ctagcttttt gcagtccgct tgcagtcaat tgtacacctt
    42001 gataagggga atgttctaca agctcttctt tcacaagttt agaaatcatt tcactgacag
    42061 atgcagctga gacatctaga ctagatacga tttgtttatt gttgactttc gttgtatctc
    42121 cacctagttc taaaatgatt ttcaaataat cttctcgatt tggtgtcata ttcgatcatc
    42181 ctttcatggt aaaaaaccat cattagtaag tgtaacaaaa aaaacgttaa ttgactattt
    42241 ttctaaataa aaaacaaagg atttttagac gtttttacaa caaaaaacga aagatgatag
    42301 agaagactca ttctttcgtt tataaaggat atatatatac attgggaggg atctattttt
    42361 cagataacaa cttatatgaa gtgtctgcag attcgtcata aagagtttgt tcgggataag
    42421 aaaagataag ataaaaacca tactgatcac gtacgactaa acgatattca tctatttcta
    42481 ccacagatgt gccaagagac tgaccgttga tcaaaaacgt gtaatcagat gtaatctgta
    42541 attgttgttt ttgctggcta tcactaaaga accatgtacc ttgatacttt gagagtcctt
    42601 ctgacttttc cgcttgtttt ttctttttca agtaaataaa acttgagaga acggtaatga
    42661 gtgaaccaag aaaatagatc atttttttta tcaccaaatc actcctttat gagaaaataa
    42721 tcgcatagac atattctttc ttctttattt cattgtaccg aataaaagct aaaatttctc
    42781 taagactttc gactggtttt tttactgttt ttctacaaat tatgatagga tacatagtag
    42841 gaagaaaggt gacataattt gaagtatcag caagtggaaa tagctttttt tattgaccgc
    42901 ccgaatcggt ttatagcaca ttgtatgaaa aaagacggag aaataattat tgcgcatgtc
    42961 aaaaatacag gtagaggaaa agaggtcctg ataccaggcg ctgaagtggc tattgtattt
    43021 gctcctggac ctaaacggaa aaccgcctat gatctgattg ctgtaaaaaa gaagggtgac
    43081 tggtttaata tcgatagtca attgcctaat cggctggcaa ttgatggtat tttagacggc
    43141 acgatccttt taccaggatt aactggtcca tttacggtat acaagcgtga agtaacttat
    43201 cttcactcca aatttgatat ttatttagag acggtggtcg gacaaaaagc atttgttgaa
    43261 gtcaaaggta tgacacttga aaataaagcg attggtgctt ttcctgatgc gcccacactc
    43321 agaggattga aacatatagg agaattaacg tatgccgcac aagatggtta tgccgcttat
    43381 gtcttgttta ttgctcagtt cgaacatctt catctggcaa cgatccatga agaaatgcaa
    43441 ccagcactag ctgatatggt tcgacatgcg cagcaaagcg gagttcaaat cctcgcctat
    43501 aattgtcaag tatcggcaga tgaggtaact gtcaagcagg ccattccatt tgatgtagat
    43561 atcccttttg aagatcctat acaatagaaa gaaggtaaaa taatgatcaa cttaggtatt
    43621 atcggaacaa attggattac tcatcaattt gtccaagcag ctttaagcac aaaaaaatat
    43681 gaattgacag cggtatattc tagacatttg gaaaaagcac aggaatttgg ttctcattat
    43741 gaaggggata tcgaattcgc aacagatttg aagacttttt tcaatattac tcatatggac
    43801 accatttata ttgcttctcc gaatagtctt cactttagtc aagctaagca agcaattttg
    43861 gcaggaaaaa atgtgatagt tgaaaagcca gctttttcga cgccaaaaga aatggatgaa
    43921 atcatccatt tggcaaatga acacaatgtg tatttttttg aagcagcaag aaatatccat
    43981 gaatcaggtt ttaaaaagat tgccgattac ttaccattaa aagacgagat aatcggtgca
    44041 aatttcagct atatgaaata ttcttcgcgc tatgaccaag tattagatgg agaagaaccg
    44101 aatatcttct cgactcattt ctcaggtggt gcattgatgg atttaggtgt ttaccttgta
    44161 tatgcagctg ttgcttggtt tggaatgcca aaagaagtcc attatttccc aagaaagatt
    44221 tctactggtg tagatggtct tggatggggg atcttacgtt atgagaattt tgatgtatcg
    44281 atccagccag gtaaaatcgg tgattcttat ttaccgtcag agatttattt tgactcagga
    44341 acgctagtga tgaatggagt caatgcaatt gaacaagcac aatttcatga tcgcgcgcat
    44401 aaagaaatca ttgatttggc ggtaacagct gcagaaaatc caatggcaga tgaagcagag
    44461 gattttgcca atgtcatggc tcatccgaaa gatccagctt ggggattgtt gtatgaagag
    44521 tggatcgagc tagctagaaa tgtgaaccaa gtgatttatg atttaagaaa aaatggcggc
    44581 atcaaattcg atgcagataa tgaggaagat ttttaatgaa cgaatttgta caagtatttc
    44641 ccgaaaattg gcagaaaaaa tatgaggata aagggtttat ctcaccttct cttattcaac
    44701 gagcagtttt tcagccacta agtaataaac aaagtatagt agcggtttct cctacaggtt
    44761 caggtaaaac actggcttat ctttggcctc tactactaaa cgtggaacca ggtgaagcaa
    44821 gtgcgttggt catttttgct tcttctcaag aactagctat tcaagtagca gatgttgcac
    44881 gtgaatgggg aaaagacaaa gagttgaaag tccaatcttt ggtgggcgga gccaacgtca
    44941 aacgtcagat tgagggtttg aaggaaaaac cagaaatctt gattggtaca ccaggaagaa
    45001 tattggaatt gatgaaagca aaaaaaataa aagcacatca agtgaaaaca atggtatttg
    45061 atgaagcaga ccagctattt gacgctggga acagccaaat cattgatcaa attcttcatc
    45121 aagctccaac agagtatcag ctagcttttt tttcagcgac agcagatcgt tcccttgaaa
    45181 gcattgaaaa aatcactggg aaaacgtttg agacaattga cgtgacagca gaggacgatt
    45241 ctcgaaaagg actgagacat tactttttac gtgtaccgat acgaaagaaa gatgactact
    45301 tgagacgtct cacgcatata gaagattttc atggtctgat tttctttaat caactgactg
    45361 aattaggcgt aatggaagaa aaattgctgt atagaggtgt accggtagga agtctggcct
    45421 ctgatcagaa caagctactc cgaaaagcag cgctagaaca attcaaaaag aaaatggtca
    45481 gagcactttt gaccacagat gtggcggcta gaggtttaga tattacaggt cttccttatg
    45541 tagtgaatgc agaagttccg ttaagtgaag aagcttattt gcatcgttcg ggaagaactg
    45601 gtcgaatggg taatgaagga atagtaatta cactcgtaca agataataat ctaagagatt
    45661 taaaacggct tttacgtcca tcaaatataa cacttgaaga gattttttta tacggaggta
    45721 agctgcaaac tgagcagcca accaaagaag atgcagctcc tgcagtaaat aaaaagtatg
    45781 cgaaaacttt ttctggaaag gagcagaacc cagaatataa caaagaaaaa ataagaaaaa
    45841 aagttaaaaa taaatcaaaa aaagagagaa ataaaggagc aaggcgcaaa taagcgcctt
    45901 tttatgctat gattatgaaa acgggaacaa aattggttgc cagaggaggg gttatgtgaa
    45961 agaacgtcaa gaagagatct tgcagctatt gacagaaaac caacaaggat tgacagcaag
    46021 tgatgtagct gagcgcttaa ctatcgatcg aagcaatgca agccgatatt taagtgaact
    46081 atacaaggcc catcatattg taaaaaccgc tggccgacca gtcgtttact ctttgccaac
    46141 ggaaaagtca aaatctgatg aagtgcatgt cgactcttct acgcaagtga cttttgaaac
    46201 actcgttgga gaaaatgatt ctttaaaagt cagcatacag caggcaaaag ctgcgattct
    46261 ttatccaccg cgggggttgc atacgattat ttttggagaa actggtacag ggaagtcgat
    46321 gtttgctgaa tgtatgtatc attttgcaat cgattcagag atgctttctg cagatgcgcc
    46381 atttgtttct ttcaattgtg cggattatgc tcaaaatccg caactgctct ttggacacat
    46441 ctttgggatc aaaaaagggg cttacacagg agcagctcaa gatagtcccg gtctaatagc
    46501 caaagcagac ggcggtatat tgtttttaga tgagatccac cgtttaccgc cggaagggca
    46561 ggaaatgctg tttactttca ttgataaagg tatttaccgt ccgcttggag aaagtgcaca
    46621 agtacatgaa gcatctgttc agatcattgg ggcgacgact gaatcttcag aaagtttctt
    46681 atctacgttc aatcgacgga ttcctatggc aatcaccttg ccgaatcttg cttcacgttc
    46741 actggatgag cgttatgaaa tcatctctct ttttatcaaa caagaagcga atcgtttgaa
    46801 tcaacgtatc gatatcgaaa aagaagcgat tcttgccttc atgctttatg atgcggaagg
    46861 aaatatcggt caagtgaaac gagacttgaa actagtctgt gcaaaatctt ttttacatta
    46921 ccgtactcat catgagaaaa agctaatcat tcgaaaagaa gaactttccc ttcaagtaca
    46981 aaaaggatta ctgaaaatca aggaaatacc ggaacgtctc gaccgattca tggacagcaa
    47041 aagtcagtat ttgacatttg aaccaggatt tgctgatgtt gtctggtcac aagatccaga
    47101 acgaaacatg caggtctata acgatattga ggaaaaagtc acttctcttt ccgaaaccgg
    47161 tgtggagaac attgatctag agaccctcat ttcaaaagat gtggatgctt atttccagac
    47221 atatgtcaaa gaattgacta aaagttcgat tcccaaagat cttttgccag atgaaatctg
    47281 gcggttaacc aatcaattgt atgatgtggc tgaaaaacgg ttagaccgta tttataatga
    47341 aaaagctcgt tttgcttttg ctttgcatct gcaaagtaca ttggatcgtg tgagagaagg
    47401 tcatatgatc gtccacccag atttgaataa cgtacggaaa aattttaaga gggaattcca
    47461 ggtagcaatc gatctttcaa cgatcattga agaagaagaa caagttgaga ttccttttga
    47521 tgaaatcggc tttattagta tgtttctttc tatcaatcta ggagaaagtg agcctgttaa
    47581 agaaaacaaa gtagatgttc tcatattgat gcacggcaga cagactgctt caagcatgtt
    47641 ggagatggca caagaactat taggaacaaa agtagggaaa gcttttaata tgccactggt
    47701 tatggaagtt caaacaatgt atgaagaagt gttacaatat gtcaaaaact atcaagagca
    47761 tttgacgaat ggcctactct tgttaacaga tatgggatca ttaaacacct tcagtgattt
    47821 gatttatgaa gagactggca ttcggacgaa agctatcacg atgacaagta ccatgattgt
    47881 gatagaagcg atacggatgg caagtgttgg aagaagctta gaagatattt atcaaaatat
    47941 ccagctttcc tttgaatcga tcgtacgtga tcagttcaaa gcggaaaagt ctgaaacttt
    48001 acagcgtaaa aaagccgtaa tcgtgacatg ctttactggt gaaggagtag ctgccaaatt
    48061 atatcagcgt attatgccag tgattaatca aaataaggta gaactgatcc agatgcagtt
    48121 tttagaacgt gaatcattca aaaaacacat cgatgcctta ttggaagaat atgagatcaa
    48181 agcaattgcg ggaacagttg atgttgatta tcaaaatatc ccattttttt cagcctatga
    48241 cgtttttgat gatgaaaaac taaatatttt aaagcgtata gctgatgatg agatcccgac
    48301 agctaaaatt gtcgattcac ttgctggatc attaagcgat gtcttttcta ttgatgaact
    48361 gatccgttca ttgcaaaaga cagttcagca gatccagacc gatttgtcga ttatcgtgga
    48421 acctggagta gatactggaa tcgttatcca tttagccttc atgttagacg gattgatcaa
    48481 acaagaaagc catcggcaat ttgagaaact gtctattttc tcaaaaattt atcgtttgga
    48541 aatggatatc gttcgggcaa acttgatggc actagaacga aaatatcagg taaaaattcc
    48601 agaagatgat atcgcatttc ttacacaaat gtttcttgaa aataaaataa aaaaatgaac
    48661 acactaatca tactgtttac acactaaaca tctgtgtgcg cactataaaa aagagctaaa
    48721 aagcatgaaa acgctatttc agctcttttt tttattggca tgcttcttgc gttatataaa
    48781 agtgtaaaaa aataaaagga gtgttaaaaa tggcaaaaaa aacaatcatg ttagtttgtt
    48841 cagcagggat gagtacgagt ttattggtca caaaaatgca aaaagcagca gaagaaaaag
    48901 ggatggaagc cgatattttt gcggtctctg cctcagatgc agataataat ttagaatcta
    48961 agaatgtcga tgtattatta ttaggtcctc aagtacgttt catgaaagct caatttgaac
    49021 aaaaacttgc gcctaaaggt attccattag atgtcatcaa tatgcaagac tatggcatga
    49081 tgaacggaga aaaagttctt gcacaagcag aaaatctaat gaaataaatc aagtatgcag
    49141 ttgggggtat aacaacgtgg aagatcaaca aaatctagaa gccatcatgg ggttaatcat
    49201 gtttggcggt aatgcaaaga gcgatgcaat ggaagcgatt gcggctgcta aaatcggtga
    49261 ttttgagtta gcggatagga aaatcgctga tgcagaagaa tcacttatac aagcgcatca
    49321 tgcacaaaca gacatgttga cacaagaagc acaaggaaat catatgcaag tgacattatt
    49381 gactgttcac agtcaagatc acttaatgac atctattgcg tttactgatt tagcaaaaga
    49441 aatcatcgaa ctttatcgtc gcgtatacag cgaataaata caacttcttt tttgaacgga
    49501 agaagaaaaa tttttgaacg gattttttat tttgttataa ggagaactct ctttatataa
    49561 aaaacttata ggaggaagta aaaaatgaac ggactatcaa gttggatgga gaaatacatc
    49621 ctgccggttg ccgcaaaaat cggagcgcaa aaacatttag ttgctttgcg tgatgctttt
    49681 atcggcacac tgcccgcaac aatggcggga tcagtcgctg taatgatcaa tgcaattatt
    49741 cgtgatttac cgcaacagtt taacagtacc tatgctgaca gtttagcagc agacaaagga
    49801 atctttgccg tattgaatgt catcatcgga atcaatggtt ttgtctggaa cggtacacta
    49861 gcaatcgccg gattgatctt tgccttttct tgggggtata atcttgcccg agcttatgga
    49921 gtaaatgatc tagctggggg gatcgttggt ttagctacct tgattcaagg tatttccttt
    49981 ggtccagcat tatcagcagc attagatgtg aatgttcctt caaatgtcgc agatgcaatc
    50041 aatagttctg agattggtgc aacgattgca gatggcacac tgtcagttgg attatggggt
    50101 tggctaaaac tagatcattt gaatggaaac gcttatttta cagttatgat catgggagct
    50161 atttcagtca ttattttttg taaattgatg cttgccaaca ttacgatcaa aatgcctgat
    50221 tcagtaccac cagcagtatc taaagcattt gcagccattt taccagcgac gatcgcttta
    50281 tacgtcattg ctattatcaa ttatatcgta ggtcgtgtag ctgatgggca actgatcatt
    50341 gatttggtgc aaaaatatat tgcagagcca ttcctaggat tatctcaagg gttgggagca
    50401 gtattgatcg tcacagtatt tgttcaagta ttctggttct ttggtattca tggaccaaat
    50461 gtattggcac cagtgctaga gggaatctgg ggacaagcac aactaatcaa tattgatatt
    50521 ttccaaaaag gttataacgg aaaaactggt acaccagccg tgttagatgc aatcgaagat
    50581 ggaaaagctt atatgtgggt ccgcggatcg ttcgatgctt ttgcatggtt tggcggatca
    50641 ggcggaacga tcgtcttact gatagctatc ttgattttct ctaaacgtgc agattatcta
    50701 acagtagcta aattatccat tgggcctggt atcttcaata tcaacgaacc agtgatgttt
    50761 ggtctgccaa tcgtattgaa tgcaatcatg tttatcccat tcttgattgc tccagtagta
    50821 gcaacagcaa ttggatggac agctacttat cttggcctag tcgctccggt atcgcagcaa
    50881 gtcacatggg tagtaccgcc gttcttattg tcattcttag caacaggagc ggattggcgg
    50941 gcaccgatcg taacattagt atgtatggtc gtgactttct tgatttgggc accatttgtc
    51001 atctctgcaa acaaaatgga ccctgccgca ggcgaacaac aataagatga acgtgaggag
    51061 cgggacgctt taagtttcgt tcctcataat gaattttcga ggaggaactg aaatgattac
    51121 aattgccagt gcaatgcaac aagatgaaat caaagaattg ttagaaggat acaatgaaga
    51181 aggaatgact tttacattta aagaaaaaca aggaatcaag ctgttctttg aaacaagtgc
    51241 agaagatccg gaagttgcgg ctaaaaaagc aaaggaacta atcaaagcac agccttgggg
    51301 aacagtcctt tattttcaat caacagctgt ttagaaaatg gtttaaaaag ataggatggt
    51361 tgagatgata aaaaacggtt tatttttatg tagtattggt tctttgatcg tgttgtatag
    51421 tttgaatcca cattcgcaaa ggtatgattt gtatagtatg gtcacaggtc tttttctaac
    51481 aggattagga atctatttct tcatgaaggg gaaaaaaaga gaagaagcaa aggagaaaaa
    51541 taactaatgg aaatcacgaa aaaattaaat attccagcat cgttttttta cgataaagtt
    51601 atggactctg tccttttcga tgtacgtaaa gcgaccggca aatcagtaac tagaaaacag
    51661 ctgaaaaatt tggagtacgt caaacaattc tcgcaaaata accgggctcg cataaaaatc
    51721 gaagaagtca ttgaaaacca gtcttataag tttcgtacat cgacaacgaa gaatgaattc
    51781 gtcgttcatt atcaaatcaa accattggac gattcgacct gtgaggttca ttacacagaa
    51841 aaaatggagt cttatggcat gcttcaaaag ttgaatgata tgcttttagg aacaatcttg
    51901 atgtatttta aacggagacg tttcaaaaaa atgctagata tgatcgaagc aacgtattaa
    51961 atcaatgaaa catggtacta tttagttatt gaaaaatgaa gtaagctaga aggaattttt
    52021 cataggaaga tagatctttc ctgcgaatcc tcactagctt ttttgtagga aggggcagca
    52081 aaatgacaaa caaagtaaga gagctaggtg tatcttcgat tgaggaaatg tttgatttag
    52141 ccacttatgc atttaatggg gcagatacac ctgaaaggcg agaacgtttt cgtacacttg
    52201 cggagaattc ttggaattac ggattttttg atgaggagga tcggctaaca agtcaagtca
    52261 tggcgacccc ttttccagtg aatttttacg gacaagagta cctaatggcc ggcattggct
    52321 atgttgcttc ttatccagaa gcaaggggac aaggcgggat caatcagatc atggaaaaga
    52381 tccttgaaga ttgccgtgat cgtaaagtaa gcttatctta tctggcgcca ttctcctatc
    52441 ccttctatcg gcgctatggc tatgaacaat catttgataa aatcagttat caactaaaca
    52501 gtcgtgatgt gccgtttatc aaaaaaaaat ctggaaaaat aaagcgtatg gattttgaag
    52561 atgcaaaagc tgcaatcaga agaatttatg aacagatgcc ggaaaaccaa cgagctggtc
    52621 tgaagcgcga agactggtgg tatacgtata aattcaagca aaaaggcaat taccagtttg
    52681 ctttgcattt tgatgataat gaggaagcaa cgggatatat cgtttatcag cttgcaacgt
    52741 cttcctttat tattgcggag tggggatttt tggatcatga tgctttttgc agccttgttc
    52801 gcttcgtaag ttctcacaat ggtgcttttg aaacatttga atattcatgc ggctacggcg
    52861 gtaatgatca aaattatcta ttagaaaatc catttgcttc tgtaatgatc acaccttata
    52921 tgatggcgag gatcgtagat atttctttgt ttttggaaaa ttatccattt aaaaagagac
    52981 aagctgcatt tgccctacaa atagaagaag atcattatgc gaaatggaat gaaggaatcg
    53041 tggaagtaac aatcgaaaat ggaaaaaatc atctctcaaa agtagaacaa acaaaactcc
    53101 caatcgttcg aggaagtatc caacatatca cgcagctatt attagggtat cgtaaagtgg
    53161 aagagttggt attccaagaa agaatacagg tgacaaaaga atatgaagaa acgttaagct
    53221 caatcttgcc acagcaacag ccaattctta atgactattt ttgataaaaa acgggtgatg
    53281 acaatatttt tgtcaccacc cttttgtttt tcatttggca tttgaaggaa aatgttaaag
    53341 taaatcctct tttttcgtaa atttgaattt tttgttttct tttgttttag ggttttcttt
    53401 tttgtggtct tggattgctt ctttcgtaat cttttcccga ttttttttag atgaatctaa
    53461 gcgttctgcg tatacttttt cttttggctt atcattcatt ggaaaggacc tccctacatt
    53521 atttcattct acctctagtt tatcatctgt taaggactag tggaaacgtt ttgcctaact
    53581 tataagctga tttttttagc agataagctt gcaagtaaaa aacaaacaag ctacaattga
    53641 tttattgaaa ttgttcctgt agctcagtag gatagagcaa tcgcctccta agcgataggt
    53701 cgctggttca attccagtca ggaacgtaca aataacccct gtatcctttg cggtacaggg
    53761 ggttattttg tataagtaga cattgtagtt tctaaactgc tatgtcctaa acgtttcgat
    53821 acgctaagta tatttactcc ttgataaagt aaaatagatg catgcgtatg tcttagtcca
    53881 tcaataaaca gtcgaagaga aaatagggaa aaattgatta ctaaagaaaa acttactagg
    53941 tcgacggtgt agttgatttt catacttcaa tagattcgaa ttaaacgatt tcgattggct
    54001 tataccagtc ctatccaacg aaagatatat cgttcttaac tctagcggta taacttgaag
    54061 ctttacgtaa agccaatggg aatgaactgg agatgacttt ttattatttt tagtaatagc
    54121 tgatgggtta atacttacaa ctttcctttt caaagtacca tataaaaaaa cggtggggtt
    54181 gtttagcgaa ataaaggatc taagccaata gcttagatcc tttattctat gctttatatt
    54241 attttttgca tgtgtgcgaa agtatactca agcccaatcg ccatttcgga aaatcggtac
    54301 tgtagagcca tcttcacgga ttccgtcgat atccatcttg tcagatccta ccatgaaatc
    54361 tacgtgggtt tgacttctat tcaatccagc ttctgctaat tcttcatcgg acatttctgt
    54421 accgcctttc acactgaagg cataagcaga acctagtgct aagtgattcg atgcgttttc
    54481 atcaaataat gtattgaaaa agatgatgcc agattgagaa ataggtgaag ggtcaggtac
    54541 aagtgcgact tctcctaaac gacgcgcacc ttcatctgta tcaagcagtt ttgctaagac
    54601 atcttctcct tgttcggcag aaaaatcaac aacttttcca tctttaaaag taaatttcat
    54661 acccgagatg atggtgccag cataactcag tggctttgta cttgaaatgt aaccatctac
    54721 tcgatgactg tcaggtgctg tgaacacttc ttccgtcggc atatttgcca tgaatttttc
    54781 tccacgagca ttatagcttc cagcgccttc ccaaagatgg tttttaggca atccgataat
    54841 gatatctgta ccgggagcag tatagtgaag ggcagaaaat tgttctcgat tcaattcttc
    54901 tgcttttttt gctaattttt catcatgctt cttccatgct aagacagggt cttcttcata
    54961 gacacgagtc gttttgaaaa tctgatccca taatgcctca acttgttttt cttctggcag
    55021 ttctgggaag acttttgctg cccattgttt accagccgca gccacgacag tccaactgac
    55081 tttatttgct tgagttgcct tgcgtaaatt cattagtgct ttgccattcg ctgattgata
    55141 agacgctact cgatggctat ctacaccagc aaatgcatcg ggatcggcag aaacaacgct
    55201 gatcctgctt gctccttttt caagccattc atccgcttga tcgattttag attgtggtac
    55261 attctcaata cgatccgttg cagcatgtaa aagaaattct ctttggatct ggtcgtcagt
    55321 ccattgaacg ataacctcag cggctcctag tttataagct tcttgtgtga tcaatcgagc
    55381 aaggggtgcc tgttccacgc tgatctgtaa aacaactgtg tgtccttttt ctgtggctac
    55441 gccagtttct gcaattaagc gtgcatattt ttttagaagt tcattaaaat cagataacat
    55501 aataaaaatt cctcctcacc attttttctg gtaactatta gcatgatacc atttaattat
    55561 gattcaaaca atgaggcttt aaataaaaaa aggaggttat ttttttaagg ttaagtttca
    55621 tgatatatgt tactacttgc tatttcagct tgtgaagaaa atcaaaaact aattttgcat
    55681 atcaaaaaaa catgttacaa tgaaattaat caatggtgga agcgttataa aaaaattaag
    55741 gagattaaaa aatatggcaa gaaaaaagac gattactaga gaacagattt tggcagctgc
    55801 ttatgaagta gctgccaccg atggatttac acgatttact gcgagaaaca tcgcgaacaa
    55861 aatgaattgt tccacgcaac cgatttatct tgagtttaaa aatatggatg acttgagaca
    55921 agctcttttt gagcagatct atgatcattt acaatatgaa gtgttcactg ttgagcatac
    55981 agggaatacg attatcgatc ttgcttttaa ttatatccat tttgcaagaa gagagaagaa
    56041 gttgtatcgt tcactgtact tagaagaata tggtggagga aaagagatgc agaatttctc
    56101 ttatacttac tttgtgaata cagtgaaacg cgatccagct tatgtgaatc tttcagaaga
    56161 gcagatcgaa tctttgtata atgggacatg gatcatagct actgggatcg cagctttgat
    56221 gtcttcaagc atcatccatc caactgatga acagattgaa aagatgatcc aagacagtat
    56281 agatgctatc ttgaaattca aagaaccaat cgaaatcgat tgaatatact caaaagttcc
    56341 acaaatcagt aggtaacaag agatgtgtca aaataaggaa aagttcgttt ttcttacagc
    56401 gtatgcattt ttaactcccg aagaactctt ccatgaatgc aatgaataaa agagccatac
    56461 agattgaata aatctggatg gctcttttat tgcaagtata ttaaattttt gcgactagtt
    56521 ctttagcgaa agcttctaaa ttttcgatat cttcttcttc agcattcaga tccactttaa
    56581 cattttcggc tccttttgat gcgcctgttt ttgcaaaagc agcatcgaaa tcatccaccg
    56641 atttgcagaa ttcatcgtaa aaagtatcac cagatccgca gacaccgtaa atttttccca
    56701 atagatccaa ttcttgcaag tcttcataaa agtctacgat ctcatcaggc agatcgccat
    56761 catcgtatgt ataagtagct actacgcaaa tatctgcctc ctcgaattca tctgcatcta
    56821 cttgggtaca ttcgttgatt tcgacttcga tatccagatt ttcaaatgct tctgcaacaa
    56881 tatcggcgat ttcttcagtg tttcctgtca tgctagcata tacgattttt gctaaagcca
    56941 tgatgtgcct cctcgtttat aattatttaa caattatgaa ttataccaaa acatcattga
    57001 aaagaaaata aaacctaaag acacctgggg aatgtcattt ggctaaatgt atgccgctat
    57061 gttaaaatga gagggatttg taaaaaggag acgaaaatat gattacttta aaatcagcac
    57121 gtgaaattga aatgatggca gaatctggag ctttattagc agatgtacat aaaaatctgc
    57181 gtgattttat aaaaccagga atcacaagtt gggatattga ggtatttgtc agaaactata
    57241 tcgagagtca tggcggaatt gctgcgcaaa ttggctttga agggtatgaa tatgcgactt
    57301 gtatcagtat aaatgatgaa atttgtcatg gattccctcg taaaaagcct ttgaaaaacg
    57361 gtgatttggt aaaagtcgat atgtgtatcg atttgaaagg cggcatttct gattcttgtt
    57421 gggcctatgt agttggagaa tctacacctg agatcgaccg attgatggaa gtaacgaaga
    57481 aagctttgta catcggtatt gagcaagcac aagtagggaa tcggattggt gacatcggtc
    57541 atgctatcca aacttatgta gaaggcgaaa atctagcgat cgttcgtgat tttattggtc
    57601 atggcgttgg tcctacgatt catgaagaac cagcagttcc tcattacgga gaagctggaa
    57661 aagggttgcg gttgaaagaa ggcatggtta tcacgatcga acctatggtt aataccggaa
    57721 cttggaaaat gaaaatggat cctaacggct ggactgcgta tacaagagac ggcggacttt
    57781 cttgccaata tgaacataca ttggctatta ctaaagatgg tccaagaatt ttgacatcac
    57841 aaggagaaga aggcacttat tgatctatcc agtcaatagg tacgggagag gtacatgaaa
    57901 gtttggaaca aaataaaaca aaatgaaaat ctgatgcgct ttattgagac gactcaaaag
    57961 cgcatcactg attcagaaat gagtacgacc tctgtcgtcg ttgcttatta tttactcttg
    58021 tctttatttc cattgattat tgcttttggt aatattttac cgtttctcca catcgatcaa
    58081 gagactgtac tgacctattt acgagaagtg attcctgaga ccgtttacca gtttatcggt
    58141 cctgcaatca aagacctttt gacacaaagt tcaggaggct tgttatccgt ttctgcatta
    58201 gcggctttat ggtctgcaag tcaaagtatc aatgctttgc aaattgcgat gaataaagcg
    58261 tatggcgtag aaaatagaaa aaattttttg atcgtccgtt tcttctcact aattgtcatt
    58321 gtcttgttca tgattgctat tagtggggta actatcgttc ttgggttggg acagctgatt
    58381 ttggatgcta ttcagccaat cgtacaaata cccgtgaact ttatcgatca gttccaaact
    58441 ttgaagtggc caatcacatt ggtagcttta tttgtcatca tgttcttgat ttatttaatt
    58501 gtccctaatg ctcagttgaa gttgaagacg atcattccgg gcacgatctt cgctactgtc
    58561 ggctggatgc tgttatcaca agtgtttagt atctatgcta ggtattttgc aacgaggatc
    58621 agcggttatc agatcatagg aagttttatt gtactcatgc tttggcttaa ttttgctgct
    58681 acgatcatca ttttgggcgg aatcatcaat gcagttgttc aagaatacgt gacgggtcat
    58741 caaatcaaaa aacgtcgaga tccaagccga aaatggttca ataaattgaa aactaaattt
    58801 tctcataaaa aaaagtagaa acaagagttc ttattgtaaa tttatatcag taatttataa
    58861 taattatgac tcttaattaa agtaggtgcc catatgcttg tttcttttag tatggttata
    58921 agattttttc tgacaatttt attatcactg atattaacac ctgtattcaa aatcatttca
    58981 gtacaaacag gtatggtcga caaaccaaat gaaagaagaa taaataaagt tccaatgcct
    59041 tcagcgggcg gactgcctat ctttatttca tttgttatat cgactttatt cttattccgt
    59101 aaaattatcc ctcaatcgta tattgtaccg atcttgattg cttctttagt aatcatagtc
    59161 acaggagtgc tcgatgataa atatgagctg acacctaaac aaaaaagtat agggatcttg
    59221 attgcttctc ttattattta ttttgtagca gatatacgta ttgattcctt cacgcttcct
    59281 tttattggat atatccaact aggctggctt agttttcccg taacgatttt ttggatcttc
    59341 ggtatcacga atgcggtcaa tctgattgac ggtttggatg ggctggcggc agggatttca
    59401 ttgatcggtt taattacgat aggaatcatt ggatattttt tcctccatgc ttctacagtg
    59461 tatattccag ttgttatttt ctgtcttgta gcaagtatta tcggtttttt tccttacaat
    59521 ttttatccgg caaagattta tttaggcgat acaggagctc tattcctagg gtttatgatg
    59581 gccgtccttt cactacaagg gctgaagaac gtgacttttg tttcatcgat ttctttgctg
    59641 atcgtcatgg gagtgccggt aacagacacg ttttttgcta tcattaggag aaaagcaaac
    59701 cgtgtttctt tttctacagc agataaaaaa catcttcatc accgactaat cagtttaggc
    59761 tttactcata aaggtgctgt cctgacgatt tatgcgatgg ctttgatgtt ttctttcaca
    59821 gctatggtca tgaattatac tggaagaatc ggaacgatcg tattgattat tgccatgttg
    59881 tttgctgcaa ttttattatt tgaattgatt ggtctgatca atgagaaaca tcagtttatt
    59941 ttaaaaacat tgcgattttt agggaataaa gagtaccgta cgcaagtaaa acagaaatat
    60001 ttgaagaaat ggaaccgtca tatagatgaa taacaaatta aaccagccga tccttattag
    60061 tattgtgacg gcaaacagtg ataaaatttt tcggacacta gataatttta tttcaactgt
    60121 aaaagatgaa acagcagtaa aaatccgaat ttttgacaac aattcacttc ccgagtacaa
    60181 aaaaagacta acagagtata ggaaatatcc ctacatcgat atctattttc atgatgaaaa
    60241 ttccggattt ggttatggac ataatcacaa tctgatagct agcgattctc gctatggcat
    60301 tatttgtaac ccagatattt tagtggatga agagacagtt acagcattgg tcttgttatt
    60361 gaaagaacat ccggaatgtg cgatgttagc acctaagatt cttaatgaag atggaacaac
    60421 tcagcacctc atccgtgagc agctggctgt ttttgattac gttttgcgct ttgtgccttt
    60481 caaatgggtc aaaaaaaagt ttgacaaacg actggctgtg tttgaatgcc gaggattgcc
    60541 tactgatgct aatagttatg tacggatgat ctcaggtagt tttatggtag ctgatattga
    60601 taaatttaaa gagataaagg ggtttgatga gcgtttcttc atgtactttg aagacaacga
    60661 cttgtgtttg agttttgaaa aagcaggtta caagttattg tacacaccct tttatgcagt
    60721 tatccatttt tacgggaagg gtgcgcatcg aaatgcaaaa ctgtttcgcg tgtttttgac
    60781 ttctatgatg aaatttttca ataagtgggg gtggcgtttt ttctgatgaa accagaaata
    60841 ttagtagtcg ttgtgcttta tcagcaacta ttcagtcaat ctccagctta tggaccatta
    60901 agcaaagcac ttagcgaaaa gcaaattcag cttattattt acgataatag tcctcaaagg
    60961 cagcaaaatg agctgtttga aaaagaaaat atcgtttatc atcatgatcc gcaaaatcct
    61021 ggtttagcgg tggcttataa ttttgcgttg tctcaagcag atgaaggaac acgttgtctc
    61081 gtcactctgg atcaggatac gaggctagtc gacgattatt ttgaactatt gcgtaaagta
    61141 acctttacag atgaatgtgt tgctgcagtt ccgatgattt tttctgggag ccgacaagtt
    61201 tctcccctat attcagatcg ttatatcaat cgtacagctc aagcagtaga agttgaaggc
    61261 ccaacagctg aacgaatcat ggcaatcaat tcaggtgttg cctggtctgt caaatttcta
    61321 aaaaagattg gcgggtttaa tcctgctttt tctttagatt ttttggatca ttggctgttt
    61381 tggaaagtgg atcagttgga aaaaactgtg gagatccttc ctgtacgaat ggaacatgat
    61441 ctttcggttt tagactatga aaaagtttca agtaatcgat accgttctat tttacaagca
    61501 gaaaatcgat tttatcatga atatgatcaa aggcatttat ccagtcaccg acgacaatta
    61561 ttgttaagaa cagctaaaca gttcttgacc gtcaaaaacc ggcatatctg gcggatgact
    61621 ttgcgctcat atgttaagaa ttggaaggtg tgaattcgtg cgttcggttt gtatagctac
    61681 ctttaatgga ggcagatatt taagagagca gttagatagt attttatctc agctttcagc
    61741 agaagatgaa gtagtcattt ctgatgatgg gtcagtagat gatacatggg atatattaac
    61801 tgaatacgca gaaaaagata agcgaatcca gcttttttcg gggcctcgtc aaggattgat
    61861 cgctaatttt ggttatgcga tccagcagac gaaaggagat ctaatctttt tggcagacca
    61921 agatgatgtt tggttggaga caaaagtgaa aaagatcact gactattttg agcagaatcc
    61981 ggatcagcta gtagtagttt ctgatttaat cattgtggac agcgagctgc aagagatcca
    62041 tgattcgtat ttttcgtttc gaaattccaa agaaggatgg cttaataatg tgataagaag
    62101 tcactatatt ggtgctggta tggctttccg gtcttcttta aaacccgata ttttgccgat
    62161 cccgtcagaa gtgccgatgc atgatatgtg gattggtttg ctgggaggaa agaagactgg
    62221 gttcctaaga gaaccgttaa ctctgtaccg aagacatgat ttaaacgcga gtgagattga
    62281 aacacattct aaattacggc aaaaaatttc ttggcgtgtt catctgatta cggcgttgat
    62341 caaacgaaaa tggtttaaac gttgataaaa gatgtttaaa tccttaatat ttccatgaca
    62401 aatataacaa ttgatgagat aataacaaag tgaaatttga aatcaaaaag gagagcagat
    62461 atccatgaag ggaatcattc ttgctggggg cagcggaaca cgtctgtacc ctttaacaaa
    62521 ggcaacatcg aaacaattga tgccgattta tgacaaacca atgatttatt atccaatgtc
    62581 tactctgatg ttggcgggaa tcaaagaaat tctgattatt tcaacgccac aagatacacc
    62641 acgtttcaag gaattatttg gaaacggaaa tgatttagga ctgcagatcg aatatgcagt
    62701 acaagaaagt ccagatggat tggcacaagc gtttatcatt ggagaagaat ttattggaga
    62761 tgatagtgtc tgcttggtat taggggacaa tatttactat ggtggcggtc tgtctaaaat
    62821 gttgcaacga gcagcttcaa aagatacagg cgcgacagta tttggctatc atgtcaatga
    62881 tccagaacgc tttggtgtag tagaatttga tgagaatatg caagctttgt cgattgaaga
    62941 aaagcctgct cagccaaaat caaattacgc tgtaactggc ctttattttt atgacaatga
    63001 ggtaatttct atcgctaaag gtatcaaacc ttcagaacgt ggagaactag agattacgga
    63061 tgtcaataaa gcatatttgg ataaaggaaa actatccgta gaacttatgg gacgcggatt
    63121 tgcttggctt gatacaggaa cacatgaatc attgcttgaa gcatctacct ttatcgaaac
    63181 aattgaaaaa cgtcagaact taaaagtggc ttgtttggaa gaaattgctt accgtatggg
    63241 ctacatcgat aaggaacaat tgctggccct tgcacagcca ttgaaaaaaa atcaatacgg
    63301 tcaatattta ttgaatttgg ccgctgaata aggagtcctt acagatgaaa gtaacagata
    63361 caaaattaac agatgtcaaa atcgtagaaa tggacgtctt tggcgatcat cgcggtttct
    63421 tcacagaaag ttattcaaaa gcaaaatttg cagaacatgg attgaaatat gactttgttc
    63481 aagacaacca ttcactatca gctgaagctg gtgtccttcg ggggcttcat ttccaaaaag
    63541 gagaagctgc acagacaaaa ttgattcggg tagtcactgg agcagtttta gatgtagtag
    63601 tggatatccg caaaggtagc ccgacttatg gtcaatggga cggatttatc ttatctgaac
    63661 ataatcatcg acagttgctt gtacctaaag gatatgccca tggattcgtt actttgacac
    63721 caaatgtgaa cttcctttat aagtgtgata attactataa tgcagaagca gatgggggca
    63781 tcgcatttga tgatccagat ttagcgattg attggccaat tgatccatca agagcgatta
    63841 tgtcggaaaa agatcaccgt catccgacat taaaagaatt tgaagcagaa aatccatttg
    63901 tttacggaga aatctaagaa ttaaggagaa attttcttat gaaaaacatc attgttaccg
    63961 gaggtgccgg attcatcggc tctaatttcg ttcattacgt ggtaaacaat catccagaag
    64021 tgcatgtcac tgttttggat aaactgactt atgcaggaaa caaagagaac ttggccggac
    64081 ttcctagcga tcgcgtagaa ttggtcgtag gagatgttgc agatgcagaa ttagtggacc
    64141 gtctagtaaa agaagcggac gcagttgttc attacgcggc agaatcccat aacgataatt
    64201 cattaaaaga tcctttccca tttgtacaaa cgaatatcat tggtacgtat acacttttag
    64261 aagcttgccg caagtatgat gtacgctatc atcacgtatc tactgacgag gtttacggcg
    64321 atcttccatt acgtgaagac ctgccaggac atggggaagg cgaaggagaa aaattcactg
    64381 cggaaacacc atataatcct tcaagtccat attcttctac aaaagctggt tcagatctgt
    64441 tagtaaaagc atgggtgcgt tcttttggct tacgtgcaac catttcaaac tgttcaaaca
    64501 actatggtcc ttatcagcat attgaaaaat ttattcctcg ccaagtaacg agtatcttaa
    64561 gcggtattcg tccaaaatta tatggcgaag gaaagaatgt tcgtgactgg atccatacga
    64621 atgatcattc ttcagcagtg tggctgattt tgacgaaagg cagaattggc gaaacctatc
    64681 tgatcggtgc agacggtgaa gaagataata agaccgtgat ggaattgatc ttagaaatga
    64741 tgggccaacc aaaagacgca tacgaccatg taaacgaccg cgcagggcac gatttgcgtt
    64801 atgcaatcga ttcgacaaaa ttgcgagaag agctaggttg gcagcctgag tttacgaact
    64861 tccgtgatgg gttggctgac acgatcaaat ggtacgaaga acatgaagac tggtggaaaa
    64921 aagaaaaaga ggccgttgaa gcagcatatg ccaaaaatgg acaataatac agaggaaatt
    64981 tcactgtatt aatggaaaaa cacatgtgag gcaggcgctc gcctgtctct tttttaaaat
    65041 aaaaatcaag gagcggataa ccatatgttt ttattaactg gaggaaatgg acaattaggt
    65101 acagaattgc gccatttatt agatgaaaaa aatctgccat atgtttcaac agatgcaaaa
    65161 gacttggata tcacagatgc tgagaaaaca atggcttata ttacagaatt aaaacccgat
    65221 gttattttcc attgcgcagc ttacacagca gtagataaag cagaagaaga agcaaaagaa
    65281 ttagatgaga aaatcaatgt cgatggcaca agaaatgtag cacttgcagc aaaagctgtg
    65341 aatgcacaat tcgtctatat cagtacagac tatgttttcg atggaaaaaa caaaaatgta
    65401 gaatacgaag tagacgacca aacgaatcca ctaaatgaat atggtcgtac gaaattatta
    65461 ggcgaaaaag cagtagaaga gattttagaa gactattata tcatacgaac ttcttgggtt
    65521 ttcgggatct atggacacaa tttcgtcttc acgatgcaac gcctagcgga aacccgcgac
    65581 aaactgactg tagtaaatga ccagtttggg cgaccaactt ggacacgtac tttggcagaa
    65641 tttatggtat atgtcatcga agaaaaagca ccatttggta tttatcattt gtcgaacgaa
    65701 aatagctgtt cttggtatga gttcgcaaaa gaaatattga aagatacaga tgtagaagtt
    65761 gcacctgtta cgtcagaaga atttccgcaa aaagcaacac gtccgcagta ttcagtcatg
    65821 agcctgaaaa agacagaagc gttaggtttt gtcataccaa cttggcaaga agcacttgcg
    65881 caaatgctgg aagctgccaa aaaaatgaat aagtaatttc ctttttatca aatagaattt
    65941 ggagcatgat atggaaaaac aaacaagaag tgcacgacga aaagaaaagc caaaagcgaa
    66001 tcaggcagat tcgaatttgg tgcttgacct gtttctaaaa ctccgctatc ttattggcgt
    66061 tttagtcatc gtggtcatcg ttacttttaa tttaaacgga agttcacttg gtgtttggga
    66121 taaatatgtg tcgcaaagag acgatgggaa aaaaactgat gtgatttttg gacagaatcg
    66181 ggaagtgcgg tcagacgaat ggctggtgca gacacctttt tacctctcac aagctgaaga
    66241 ggacttttcg ttggtgaacg aggattattc attaaacgga caaaacatga tcattgcgta
    66301 taattctcct gtaaaggata ttacggttat cggaaaacca ttcaactggg gattcttttt
    66361 cctcggaaga gatcgaggac tttcctttta ttgggcgatg aaattagtcg tcatggtctt
    66421 gcttggattc gaagtactga tgatattaac gaagaggaaa aaagctctat cgctaatcgg
    66481 tgcatttgcg ctgacatttt ctccagcagt ccagtggtgg ttcatgcaac atgtgggcga
    66541 tttgattttc tttactttgg gactgatgat tgctttttat cattatttct acaatcatga
    66601 taaaaaatgg ctacgaacgt tgatgatgct tttggtggtt attttcggta ttgggtttat
    66661 tttagtgatt tacccagccc atcaagtgat gctggcgtac ttgctgattt tttattttgt
    66721 tggcctgctt atttactata gcaagaaagt ttcatgggat ctgttggatg tagtgttgat
    66781 tggaggggca gttttattta ttggcggagt catggttcat ttctggctaa cgtcaaaaga
    66841 cgcattgtta gcttctttga ataccttata tccaggaaaa cgggtaagta ctggaggaaa
    66901 gtggacgatt ggcgaatttt tttctttcct tacaaattgg aaaattcctt ttgaagatat
    66961 caactattcg aataactctg aagtggccct gttctatcac ttttttccaa ctgtattcct
    67021 agcaagtcca tttgtgttat ttggcaaaaa agatagcgaa caaaagttac tgggacgtat
    67081 tttgatgatt ttttgtctgt tttctatctt ttggattacc gttgggttgc caaaatggct
    67141 agcagaaatc actctgctgt cttatgttcc tccattaaga gccatcctta cttttagttt
    67201 tgcggcttgt ttattatcga tctggtttat cgcttatatt tggagagaag atgttattcc
    67261 tatttggtat aaattcattt tgttggcagt aaatgcatgg tcttatttct atgcgattac
    67321 gcattcgaaa atggataatt atttttcgga ttttgaaacc atcgcagttg ctgtgttggg
    67381 aacagtgatt ttagcttttg cattatttgg ctggagacgc ttgttttacc tcgcttttgt
    67441 cgccttagtt atcgtatctg gctttttcgt caatccagtc gtagaaggta caggagcgat
    67501 ttttgaaaaa acactggcaa aagaaatcca aaaaatcgat caacaagatc ctggtaaagt
    67561 ttggttgtct gaagatgaat tatataactt caccccaaca ttgggagtga aatccttcaa
    67621 tacagtacgc ttttatccag atatagctgc atggaagaaa atcgatccga aagaagaata
    67681 cgagaagtat tataatcggt atgctcatac aagagctttt atcgctaagg aagatacaaa
    67741 gttcatttta gatcgtccag atatgttttc ggtcacgttg aattttgaag acgcagaaaa
    67801 attagggatc gactatgtga tcagtaaacg accattaact gattacaacc aattatatga
    67861 agcgcagttc acgcagctat atggaccgga taaagacggt tatatgattt ttaaagtaga
    67921 atatccggat tatccagatg taccacaaac acaaactcag gaaaatcagc tagtcgtaca
    67981 gtaataggct agctgagaat aatcctgaaa tattagtaga aaggagagtg aagataattc
    68041 aagcaatcgt acaaaaattt gtcaacaaaa tgagtgtcgg tttcttgctt ttgttgctga
    68101 cgctagtctt ttttgtttgg acagcagggg taggttttcc agcaactttg tttatatata
    68161 acaatagttc attacttatc gcttttagtg tggctatttt gctcatttta aatgtttata
    68221 aagcaaaagg cgtcgatttt ctttatctag gtgttgcgct tgcccttttc attttctttt
    68281 cttactttcg ggaattaaga aatgagtctt tatttggaga cttactgttt cctttggtta
    68341 ttttattttg tttagtaatt aaatggatcg agtggacaaa atgggatcgc ggtatttatc
    68401 tgattgtctt tacggtcttc ctcggaatca cgttatatcg tgtatttaca gagatccaag
    68461 taccagaagg acaaagcatc tgggctccag gcaattcgct ttctgatatt tggatcaata
    68521 cgaatacaat cggaagttct ttgatgactc ttgctttatt gatcagtgga tttgccagtt
    68581 cttttgaacg atggtatgtt cgaatcatcg gtgggatagc agtcgttgca gcctttttag
    68641 gtatttgggt ctgccaatct agaggtgcat tagtcgcgtt gattgttttt gcgatattgg
    68701 atcttttacc taaatcgttg atgcgtgtca ttcgtgcgcc atttattgcc tacacggtta
    68761 cgattctttt agctttgccg atttcttatc tggcggctgt ctctgaaaaa gtcaatttgt
    68821 ttaccgatcg agaagatatt tggcataaat tctatcagac gctaggagaa aaatctgaac
    68881 aagttttact aggaatgaaa acatttatct tccagcgagg aaaccaattt ttaggaaacc
    68941 acaatagtta taattctatt ttgaatatct atggattgat aggatttggg attgctgctt
    69001 tgctgcttat cctttttatt ggtcgcttaa cgttgaaagc agatttatcc aacgggcaga
    69061 tgacttttat ctgggccttt tttgcggtga tgatgcagtc atttatggag gatacattga
    69121 cttcataccc atgggttccg attatttata tcctgttggc tatggccgtt catcgatatg
    69181 atgaaccaga agttgcggag gaattctatg ttatggaaga ggcatatgac gatatgactt
    69241 atgatgatac atcttatgag gaacaaccag atagcgctca ttcttccagg atccaacgct
    69301 atcattgata ggcacaaaat acgagagagg ctgtgacaac cgtcgcagcc ttttttggtt
    69361 tattgcaatt gtctagcatc ttctgtaaaa tagaaacgtt agagaaaatc ttgtatcata
    69421 gaaaattaag aaagaaataa gaacgcagct cggtaccaaa aatatggtaa aatggcactt
    69481 gactgtaatt cactgaaaat cgcttggagg aacacaaatg tttaaagatt tgtatttatt
    69541 tatgaaggac atttatcaga acagaaaatt attgctacag ttttcgatca atgacttcaa
    69601 atcaagatat gcaggatcgt ttttaggaat cttatgggcg tttatcaatc cgctatttac
    69661 tgtattgatt tattggctgg tatttggtgt tggactgaaa gcacaaatga tcgatgcaaa
    69721 atacccattt atcgtatatt tagttaccgg gatgattccc tggttcttct tctcagatgc
    69781 atttgcatca actacggttg tttttcggga atatacttat ttagtaaaga aagtcgtatt
    69841 caatattcgt atcttgccca cgacgaaaat cctatccaat ctgtacaccc atttattctt
    69901 cattctgatt ggcatagctg tcgcgcttat aaatggtata tatccttcag caatgtcttt
    69961 acagctgatt tattatttat tttgcatgtg tgctttcttg acaggattga cttggctgac
    70021 ggcatcgatc caaccatttt tacctgatat catgcaatta attacgatcg tgctgcagtt
    70081 gatcatgtgg acattaccga tattatggag ccctaatcag tttgattcag gtcttgtaag
    70141 catattgaaa ttaaatccgc tgtactatgt tgttcaaggt tatcgtgagt catttttaag
    70201 tgaagcttgg ttctgggagc atggaacata tactttgtat ttctgggttt tcacgttagt
    70261 aatgttcgta atcggctctg cagtgttccg tagattgaaa ccacattttt cagatgtgct
    70321 gtaaggagaa tagctatgac agaatatgca attagactaa aagatatcac aaaatcttat
    70381 aatatgtacg caaaaccttc ggatcgattt cgtgaagccc tcaatccttt taagaaatcc
    70441 tatcatgatg tattttatgc gttgaaaaac gtgaatgtag aaataaaaaa aggtgagatg
    70501 atcggtttcg tgggagaaaa cggatcggga aaatctacaa tgttgaaaat cattacggga
    70561 gtactgacac caacatccgg cacgatggaa atcgaaggaa aaatctcagc tttgctagaa
    70621 ctaggatcag gattcaatcc tgagtattct ggctatgaaa atatctattt aaatggaatg
    70681 gttctaggat tttcaagaga agaagtcgat gaaatggtcg atgatatcat tgaatttgct
    70741 gacatcggag accatattta tcaacctgta aaaacttatt caagcgggat gttcgttcgt
    70801 ctagcttttg ctgtagcaat caatgtgaat cccgatattt tgattgtgga tgaagcattg
    70861 gcagtaggcg atcttgaatt tcaactaaaa tgtatggaaa aatttacaga aatcaagaac
    70921 tcgggaaaga cgattttatt cgtttctcat gatatcagtt cgattcgtcg tttttgtgat
    70981 cgttcctatt ggttgaaaaa cggagaagtc gtcgaatatg gcgatacgat ggaagtaacg
    71041 acgaattatg aaaacttttt aaagaaaaaa tcagttaaaa cagtggatcg gaataaaaac
    71101 caagtagaac ggttcgccgc tccagatatc gtcgacgtga cgaaagcgga attgttggat
    71161 aaagacaaaa atccggtgga tatgctggaa caaggagaaa aactatacgt taaagtaact
    71221 tatgaagtaa aagatgatac aatcaaaaat ccggttttag gaattgcgct acgaacagtc
    71281 gacaattttt atgtatgcgg acttaatact ctactagatg gtatcaagat tccttggaaa
    71341 aaaggagaga atatctttta tctggagtat gaaaagttag gtttgttagc tggtgaatat
    71401 tattttgacg ttgcggtctt tgaagaaaat gcaacggttc ctttggtata taagacaaag
    71461 tacatgaact tgtttgtcag cggttcttat atcggagaag gcatcgtggt cttagaccat
    71521 aaatgggaag aagggactca tagcaatgaa atatgatttt gaaatggacc tagatgaaca
    71581 aagcagtgtg ggaaaaattg ccgcacagat caaaccagga agtaaagttt tagagtttgg
    71641 tcccggaaac ggccgattaa cgaaacattt aatcggagca aaacaatgtg aagtgagcat
    71701 tgttgagtta gataaggaat tgttcgattt tgtcagcgaa tttgctcaag atggatttta
    71761 tggtgatatc gaaagttttg agtgggcaaa ctattatgct ggacagacat ttgattacgt
    71821 cttgtttgcg gatgttttag aacatttggt agatcctgga aaaacattga agaaagtcag
    71881 agaatttttg aatgaagaag gagagatcct gattacattc cctaacttgg cgcataattc
    71941 tgtcatgatc gatttgttca ataaccaatt gccgtgggct tcttatggtc tcctagatga
    72001 aacccataat tctttttata ctcatgatgg cttccaaaaa gtttttgaaa aagcgggatt
    72061 attcattaat attgaagatt atttgtactt agctgtaggt gatacagaat taaaatcaac
    72121 ttatgaagaa ttgccggaag ctgtacgcta tgatttcaaa atgcgaccat ttggtgaagt
    72181 ctaccaatac tttttctcat tgatgaaaca tccagtagca caaagttcca ttgcagagcc
    72241 acaaaattct aattatgtaa aagtgttaga agtaacccag caaacgaaac aagatgaaac
    72301 aatccaacaa ataccattca ataatttcac aggggaaaat gagacactaa gtttccccgt
    72361 atcagaaaca acagaaagaa tggtgtttcg cttcgcgcaa caacctagct ttatcgaatt
    72421 cagcgcagaa gctgccggag aaaaactaac atttatcgat tcgaatgctg tagtcaaaac
    72481 agtaaatgat tgttatttgt ttgatgggaa agaattacca gagttcgttc taacggatat
    72541 ttctgggaaa gaagtgacta tccattgtca ctatcgcttt atcggggaat tgacaccaac
    72601 aatgaaagaa ttgttggaaa cgattcgtcc catggctgaa gtcacaaagc agctttctga
    72661 gaaaaatgac gaattagaaa gagaaaatca tcatcttctt gaggaaaata caagactaga
    72721 ccatacgtta aaaacaacca caaatcgcta ttgcacattg ttggaaagtg atgaatggac
    72781 aataaaacat agactaggac gacgtaagaa agaaacttcg aaaaaaatcc aagaaaaaga
    72841 actttctata tgtattgacg agaaaatctg ggatgcagaa acaaaaatat taaaaatcat
    72901 cggatgggga atcgctaatt cggatcgcca gcccttgtcc tataagttat cagctgacca
    72961 atcgccattt ttcgaagcaa ttcctgtttc acgagaagaa gtcaatcaag cagaacagct
    73021 cgcaaaagga acagaagcgg gatttgagtt acgaatcctt tgcgagcaag aacgttcatt
    73081 cttagtggaa gcagtagctc aaaatggcca atcatggatc attgaaatgt aaataatgat
    73141 taacttataa agataaggag taaacgaatc gtgcaaaaaa aagaaattgc cgtatttatc
    73201 gatcaaatca ctcgtgaacg ggccactggc gatttgctta ttgtgggctg ggcaattgat
    73261 gaagtgacca aagaaatccc tacgataaag gtagaaaaag aaaatgtgat tgctgaagca
    73321 acacatgtcg taagattgga tatcaatcat ttatataatc ttgatgttaa gacccaaagc
    73381 ggtttcaagg tacgtttatc agggaaaatg agaggaaaag ccatcttaga cttccaaaca
    73441 gcgagacatc aaaatggcat agctgtgaaa ttaaacggta aataccctta tgatgacggc
    73501 attgaatctt catgggaaag aaaaaaaaga ttactgaaaa aagggataaa ttatgcaagg
    73561 actcatggcg taaaaaaagc gatccgtcgg gtcaaattgg aattgaagcc aggatcgatc
    73621 gactatgcag agtggatcag tcgtcatgag aaaccagatt tgaaagagca aaggaaagaa
    73681 gcacaaggat ttgcgtaccg tccattgatt tccatactta tgcctgtcta taacgttgaa
    73741 atcaaatggc tcgaaaaatg cattgattcg gtactggatc aaacgtatga tcattgggaa
    73801 ctatgtatca gtgatgatgc ttctactgat ccagctatcc gaaagtgtct ggaatcctat
    73861 caagcaaaag atgatagaat caaagtcgtt ttccgaaaag aaaatggaca catcagtctg
    73921 gcaactaatt ctgcattgga aatagcagaa ggagaattta tagcactctt agataatgat
    73981 gatgaattac cgccatttgc attatatgaa gtggcaaaag tgctaaacgt acatccagaa
    74041 ctggatgtga tctacagtga cgaagataaa atcgatgcag atggcaatcg ctttgatccg
    74101 catttcaaag cagactggtc gcctgataca ttgatgggaa ataattatat ctctcactta
    74161 ggcgtatatc gaacaagcat tgttaaagaa cttggcggtt tccgtaaagg ctatgaaggt
    74221 tctcaagatt atgatttggt ccttcgtgtg acagaacaaa tccctgcaga tcatatttac
    74281 cacattgacc gagtcttgta tcattggcgg acgatcccag gatcaactgc aagtaatggc
    74341 gaagccaaaa gttatatcta tgattccgga gtgaaagcat tgacagatgc cctgtctaga
    74401 agaaacatca aaggaagcgt acgtcctggc agaatttctg gattttatga gattgcttat
    74461 gacgtcttgc aagaggattt ggtcagtgtg atcattccaa cgaagaatgg ctatgaagat
    74521 ctaaaaactt gtgtggattc gatcatcgaa aaaacgtctt attcaaatta tgaaatcatt
    74581 attgcggata acggaagtac agatccaaaa atgcaagaat tatttgcaga atacaagcat
    74641 cagctgaaag accgctttat cgtagaattg attgacattc cattcaatta ttctcgaatt
    74701 aacaatttag cagcagaaaa agcaaacggc aaatactttt tattcttgaa taacgataca
    74761 gaagtgattg agccagattg gatgacggtg atggtttctt atgcacagtt tgatcgaatc
    74821 ggctgtgtag gagcaaaact gttttatcca gatgatacaa cgcagcatgc aggagtatta
    74881 ttaggaatcg gtggtgttgc agggcatgca ttgaataact atgatcgaac acattgtggt
    74941 tatttcggcc gattggtcat tgacgtcaat tacttagcag tcactgcagc ttgtatgatg
    75001 gtcaaggcag ctgacttcaa tgctgttaac gggtttgatg aaacattaga agtagcattc
    75061 aacgatgttg acttgtgctt gaaagtttat gaactgggtc gttacaatgt ctatgcacat
    75121 caagcagaat tgtaccattt cgaatctaaa tcaagaggtt atgaagatac tccagaaaaa
    75181 caaagacgtt ttgctggtga aatcaaaaaa atgcaagata aatggccagc atatattgcg
    75241 catgatccat tttacaatga taatttaaca aaagacggaa tcggcgactt ttcgttgaga
    75301 ccagattaaa ctataataaa aagctgttat gttgtgattc gctcgcacat aacggctttt
    75361 tatagttttt tgtcaaaaaa aggcagaagg tgccgactca aaaaaataag tcgaggttgg
    75421 gacagaagtg tttaactccg agaaataaga agaaattcac gaaaattgct tttcaaattt
    75481 ttgtgaattt cggcttattt aagaagaagt tgcttctgct cccaccgttt attcgttttt
    75541 aaagaatgag acaaaagtgt ttcttacttt tgtcccacgc tcttattcat gctcagagcg
    75601 ccaatagata caaaatcata gccaaagttt cacgaaccct agctgtcaaa gaatcgatat
    75661 tttattttaa tataggctat tttaaaataa atggtttaag gtattcctct acaaaagcca
    75721 ctctatcaat tatcatgggt ttaaaataag aagtgatgga gataaccaga ttgttcttag
    75781 cgtcaatata aagaacattt ccactattac caattgctga atatatttcg ttctcagaat
    75841 ctaataccca ccataaatag ccatatctca tattttgtaa gtcttctaaa gcactggttc
    75901 taggtttgaa catctcgttt atccacatgg gtggtacgat ttgttttcct ttatacatcc
    75961 ctttatttag gcataataca ccaatttttg ccagatcact tgccgataaa cacaaaccat
    76021 atcctgccgt cccgatattt agtggatcac aaaaccagac attggtttta ggagacttat
    76081 ccattgtaaa ctgcttatgt tcttcagctg tttcagcata ataattgaca tggtttggta
    76141 tccctatagg atcaaataga tacttattgg caaaatcaac agttgttagc ttacttgtct
    76201 gatacaacaa gccagttaat atgtgtaaac agacagtttg atagttgaat tcgccggtta
    76261 taccttttct tcctcccaat aaatctagtg atgtttttgt ccaatcttca cttgaacaga
    76321 cttttgtcca tggatcgcct ttacatttgt agggtgcttt cattgttagc aagtgcttga
    76381 ttgtaatagc gtaaatagtt ttctccccgc gtttcacttt atactctgga aaataatcta
    76441 atactttatc atcgatactt ttgattaatc cttgctctaa agcaatgcca atcagcaaag
    76501 atacaatact tttcgttgct gacatgatat gacaagtatc gtctttttta taaccattcc
    76561 attcatctgt ataaacttct accccatcct tatatgcgac aatttgacat atatttggtt
    76621 gtcgaaaacc aataaaatca tgcaactctt gtttattcat aagatattac ctcactttct
    76681 tctataagca taatgtcatt aaaaatcgat ttcaattttt tcgattcttc acgtaataca
    76741 ggtattctta tggactagac agcgctttgg gaagatggat tctctgaaaa atataaaggt
    76801 tcactttatg agtggataaa aaaagcagca aatacttcga cttctttaat ttaccaacat
    76861 tagtgaaaat cgattcatct ggagatttgt aatttagagt ccttgtcttg attagaggtg
    76921 tataaagctt tttattagac atttttaaat tccattacaa tttgcttata aactaggttt
    76981 ttaaaggtga ctatggtaag ataatagaga tattattttg tgattaggag tgtatatatg
    77041 aataaaaatg gagaatggag tgctgcacga cggatattca ttatattaat ggatgttatt
    77101 gtatacaatg cagcaatata tagtagtttc ttactgaagt ttcgtggaga gatcccatta
    77161 cgaaatttag aaactttcca acactcagca attattattt cagtgatttt tattgcgttg
    77221 aacattttgt tgggtgcata tgttttttat aatcgaatga tcagtgacat cgtgtttgtc
    77281 acagtcatcg gtcaggtatt gatgacacta gggatcatgg tagtgacttt tgcgggccgc
    77341 tggtttgctt ttccacgtct tgttatctta ctttcatttg ttctcggtac gcttttctta
    77401 agtatctatc gtattctcgt atataaactt tatttacgcg taagtcgaga taaaaaggtc
    77461 gtcattattg ggttagaaaa ggatgtcgca ccagcaatcc aaaattttgg ttcaaagaaa
    77521 aacaacaaac ataaagtcga agcagtcatt cttgataact attatgcaaa catcaaaaat
    77581 atgattaaag acatcgatat tgtctatctt gcttctcata tcgaagaaac tgaaaaaatc
    77641 aaaatctatc aattagttac taaaaatgaa aaaaaattgt ttttgaatac aacatttgaa
    77701 aacttgacat tgatcaatcc aaatattatg aatttcgaag atgaaagtat tattgaagca
    77761 tctggattta gaatccaacc tgaatatgaa ctattcaaac gtttgtttga tatcatcgtt
    77821 tcacttgttt tattagttat tgcttcacca tttatgttat tcacagcaat tttagtcaaa
    77881 gtgacatcac cgggaccaat catctataaa caagtgcgga tcacaaaaaa tcaaaaggaa
    77941 ttttcaatct ataaattccg ctcgatgacc gccacagccg aagcaaaatc tggaccagtt
    78001 ttagctaaga gcaatgatgc acgagtaacg cctgtaggta aatttattcg agctgtccgc
    78061 tttgatgaat taccacaaat cttcaatgtg ctgaaaggcg atatgtcgat cgtaggacca
    78121 cgtcctgaac gttctttctt cgtcgatcag ttcaatgcag aaaaccctta ttactactta
    78181 cggcataatg ttcgtgctgg tatcactggg tatgcacaag tctatggaaa atatgtctct
    78241 gattacaaca gtaaattacg ttttgattta ctttatatca aaaaatattc attgatgctt
    78301 gatttaaaga tcttgctgca aaccatcaag atcctatttg ataaaatgtc ctctcaagga
    78361 cttgaagaag acgaaaatgt gatctcattg gcagaaattg cttttccgaa tgaaaaaatc
    78421 tttcgctagt tgggaggcgt aaaaatgaac aatccgaaat tcagcataat cgtaccagta
    78481 tacaatgcag aagcaactat tggtcgaacg attgatattc taaaagaatt ggattatgaa
    78541 gagtacgaag tcgttttagt aaatgatgga tcaacagatc aaacacagga aatcattcaa
    78601 gaactgattg aaaatgatga acgttttgaa ttgattacta cgaccaatca agggcccgga
    78661 ttagcaagaa acgaaggaat aagacagtca aaaggagaat atctcttatt cttcgatgca
    78721 gatgacacgc cagtaaaaac gatcttgtca gattatgatc atttgctaga aaagagtcca
    78781 aaagcggatc ttgttgttag ttcgtttgtt tttcgtacaa tggacgaggg aagaatcgtt
    78841 tccgaaaaag aaaacttagt tacagaatgt cactatctaa ctcacgaaga gtttataaaa
    78901 gacatgtatc agttaatgaa tgaacagttg atgtatgttg tatggaacaa atgctatcgt
    78961 agcgatattc tgaaaaataa ccaaattcta tttaaaactt ataatagttg tgaggatcga
    79021 atatttaact tacattatta tgaatactgt catcaagtga tcatgaaccc aaaagtagaa
    79081 tatatttatg aatttgaagg tgggaagggg atcactaatc aataccgacc taacaagttc
    79141 gaaacattta aagaattcta ttcattagca aatactgtaa cagacgatat taataaagac
    79201 ggtatggcgg cgcttctttt aaaagggaca acttcagtta ttttttctat ttatagtacc
    79261 tctgcaagaa cttcaaaaga gaagaaagac gaagcaaagc agatattgag tgatccagtg
    79321 atccgagaag ccaagcaaat tgctaagacg gattcttcag caaaaaaaat aacaaagttg
    79381 ctttacaata tgccagctcc actattttta tctgctgtaa aagttggaag cttcgttgaa
    79441 gtgaaaatgc cagggttaat ggctgctttg aagcgagtat attagttaat ggaggaaaat
    79501 ataatgaaaa gaattttgca ttttcaggga cgaatgggtt taggtggagc agagtctttc
    79561 atgatgaatt tatatcgtaa aattgatcgt acaaactatc aatttgattt tttgatttat
    79621 gaagattatg cagatgttca agactatcat tcagagattg aaagattagg aggccgtatt
    79681 tttgttgtac ctaatcctaa gaaaaatatt tttaaatatt taatagaagt gaataagtta
    79741 ctaaaaaaag aatctttttc tatagttcac aatcaggttt attttggcgg gggaattaat
    79801 ttatggttag ccaaaaagaa tggaatcaga cagagaattg ctcatagcca tgcaactgaa
    79861 gatggaaaat ctcaaaatat tgtaatgaat gtattgcgta aatttttaac caaattgttg
    79921 ttacaaaatg caacagacta ccttgccgtt agtcaagaag ctggtgaaag tctgtttcaa
    79981 aaccatccct ttgagatagt tcataatggt attgatttag agctttatag taaaaattca
    80041 gaagctaagg ttaataagag aaaaga
//
LOCUS       DACDSY010000003        63419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDSY010000003 DACDSY010000000
VERSION     DACDSY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.003"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1796
                     /locus_tag="IWK44_000183"
     CDS             <1..1796
                     /locus_tag="IWK44_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP9548741.1"
                     /translation="YSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWSPS
                     SDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQSA
                     ETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSLDG
                     LNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEAWK
                     EKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQPL
                     VNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMAKF
                     AQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHAFH
                     SSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNALAM
                     FMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKLHF
                     FIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKHLN
                     VDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(1864..3438)
                     /locus_tag="IWK44_000184"
     CDS             complement(1864..3438)
                     /locus_tag="IWK44_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548742.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(3693..4202)
                     /gene="mreD"
                     /locus_tag="IWK44_000185"
     CDS             complement(3693..4202)
                     /gene="mreD"
                     /locus_tag="IWK44_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP9548743.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(4206..5060)
                     /gene="mreC"
                     /locus_tag="IWK44_000186"
     CDS             complement(4206..5060)
                     /gene="mreC"
                     /locus_tag="IWK44_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP9548744.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(5215..5853)
                     /locus_tag="IWK44_000187"
     CDS             complement(5215..5853)
                     /locus_tag="IWK44_000187"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP9548745.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(5854..6504)
                     /locus_tag="IWK44_000188"
     CDS             complement(5854..6504)
                     /locus_tag="IWK44_000188"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9548746.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(6517..7416)
                     /gene="rsgA"
                     /locus_tag="IWK44_000189"
     CDS             complement(6517..7416)
                     /gene="rsgA"
                     /locus_tag="IWK44_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP9548747.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(7581..9650)
                     /gene="pknB"
                     /locus_tag="IWK44_000190"
     CDS             complement(7581..9650)
                     /gene="pknB"
                     /locus_tag="IWK44_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP9548748.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(9647..10387)
                     /locus_tag="IWK44_000191"
     CDS             complement(9647..10387)
                     /locus_tag="IWK44_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP9548749.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(10400..11758)
                     /gene="rsmB"
                     /locus_tag="IWK44_000192"
     CDS             complement(10400..11758)
                     /gene="rsmB"
                     /locus_tag="IWK44_000192"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP9548750.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(11758..12696)
                     /locus_tag="IWK44_000193"
     CDS             complement(11758..12696)
                     /locus_tag="IWK44_000193"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP9548751.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(12689..13180)
                     /gene="def"
                     /locus_tag="IWK44_000194"
     CDS             complement(12689..13180)
                     /gene="def"
                     /locus_tag="IWK44_000194"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9548752.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(13195..15603)
                     /gene="priA"
                     /locus_tag="IWK44_000195"
     CDS             complement(13195..15603)
                     /gene="priA"
                     /locus_tag="IWK44_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP9548753.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(15856..16161)
                     /locus_tag="IWK44_000196"
     CDS             complement(15856..16161)
                     /locus_tag="IWK44_000196"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9548754.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(16166..16780)
                     /gene="gmk"
                     /locus_tag="IWK44_000197"
     CDS             complement(16166..16780)
                     /gene="gmk"
                     /locus_tag="IWK44_000197"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP9548755.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(17267..17551)
                     /locus_tag="IWK44_000198"
     CDS             complement(17267..17551)
                     /locus_tag="IWK44_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548756.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(17569..18459)
                     /locus_tag="IWK44_000199"
     CDS             complement(17569..18459)
                     /locus_tag="IWK44_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP9548757.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(18479..18697)
                     /locus_tag="IWK44_000200"
     CDS             complement(18479..18697)
                     /locus_tag="IWK44_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548758.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(18667..20367)
                     /locus_tag="IWK44_000201"
     CDS             complement(18667..20367)
                     /locus_tag="IWK44_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP9548759.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            20634..21407
                     /locus_tag="IWK44_000202"
     CDS             20634..21407
                     /locus_tag="IWK44_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548760.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(21488..22057)
                     /gene="thiT"
                     /locus_tag="IWK44_000203"
     CDS             complement(21488..22057)
                     /gene="thiT"
                     /locus_tag="IWK44_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP9548761.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(22123..22225)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(22231..23202)
                     /locus_tag="IWK44_000204"
     CDS             complement(22231..23202)
                     /locus_tag="IWK44_000204"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9548762.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(23296..24669)
                     /gene="glmU"
                     /locus_tag="IWK44_000205"
     CDS             complement(23296..24669)
                     /gene="glmU"
                     /locus_tag="IWK44_000205"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP9548763.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(25172..25990)
                     /gene="purR"
                     /locus_tag="IWK44_000206"
     CDS             complement(25172..25990)
                     /gene="purR"
                     /locus_tag="IWK44_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP9548764.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(26157..26981)
                     /locus_tag="IWK44_000207"
     CDS             complement(26157..26981)
                     /locus_tag="IWK44_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9548765.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(26954..27634)
                     /locus_tag="IWK44_000208"
     CDS             complement(26954..27634)
                     /locus_tag="IWK44_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9548766.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(27657..28595)
                     /locus_tag="IWK44_000209"
     CDS             complement(27657..28595)
                     /locus_tag="IWK44_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP9548767.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(28840..29691)
                     /locus_tag="IWK44_000210"
     CDS             complement(28840..29691)
                     /locus_tag="IWK44_000210"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP9548768.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(29845..32418)
                     /gene="mprF"
                     /locus_tag="IWK44_000211"
     CDS             complement(29845..32418)
                     /gene="mprF"
                     /locus_tag="IWK44_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9548769.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            32559..33365
                     /gene="proC"
                     /locus_tag="IWK44_000212"
     CDS             32559..33365
                     /gene="proC"
                     /locus_tag="IWK44_000212"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP9548770.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(33444..35000)
                     /gene="rny"
                     /locus_tag="IWK44_000213"
     CDS             complement(33444..35000)
                     /gene="rny"
                     /locus_tag="IWK44_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP9548771.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(35411..36460)
                     /gene="recA"
                     /locus_tag="IWK44_000214"
     CDS             complement(35411..36460)
                     /gene="recA"
                     /locus_tag="IWK44_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP9548772.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(36569..37810)
                     /locus_tag="IWK44_000215"
     CDS             complement(36569..37810)
                     /locus_tag="IWK44_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP9548773.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(38050..38628)
                     /gene="pgsA"
                     /locus_tag="IWK44_000216"
     CDS             complement(38050..38628)
                     /gene="pgsA"
                     /locus_tag="IWK44_000216"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP9548774.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(38837..39721)
                     /locus_tag="IWK44_000217"
     CDS             complement(38837..39721)
                     /locus_tag="IWK44_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9548775.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(40499..41785)
                     /locus_tag="IWK44_000218"
     CDS             complement(40499..41785)
                     /locus_tag="IWK44_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9548776.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(41778..43046)
                     /locus_tag="IWK44_000219"
     CDS             complement(41778..43046)
                     /locus_tag="IWK44_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9548777.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            43298..43759
                     /gene="mscL"
                     /locus_tag="IWK44_000220"
     CDS             43298..43759
                     /gene="mscL"
                     /locus_tag="IWK44_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP9548778.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(43819..44265)
                     /locus_tag="IWK44_000221"
     CDS             complement(43819..44265)
                     /locus_tag="IWK44_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9548779.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(44358..44603)
                     /locus_tag="IWK44_000222"
     CDS             complement(44358..44603)
                     /locus_tag="IWK44_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548780.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(44719..45297)
                     /locus_tag="IWK44_000223"
     CDS             complement(44719..45297)
                     /locus_tag="IWK44_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9548781.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(45374..45904)
                     /locus_tag="IWK44_000224"
     CDS             complement(45374..45904)
                     /locus_tag="IWK44_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP9548782.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(45908..46768)
                     /gene="rlmB"
                     /locus_tag="IWK44_000225"
     CDS             complement(45908..46768)
                     /gene="rlmB"
                     /locus_tag="IWK44_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP9548783.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(46761..47156)
                     /locus_tag="IWK44_000226"
     CDS             complement(46761..47156)
                     /locus_tag="IWK44_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9548784.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(47153..48565)
                     /locus_tag="IWK44_000227"
     CDS             complement(47153..48565)
                     /locus_tag="IWK44_000227"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP9548785.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(48562..49098)
                     /gene="cysE"
                     /locus_tag="IWK44_000228"
     CDS             complement(48562..49098)
                     /gene="cysE"
                     /locus_tag="IWK44_000228"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP9548786.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(49420..50880)
                     /locus_tag="IWK44_000229"
     CDS             complement(49420..50880)
                     /locus_tag="IWK44_000229"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP9548787.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(51050..52207)
                     /locus_tag="IWK44_000230"
     CDS             complement(51050..52207)
                     /locus_tag="IWK44_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP9548788.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(52241..53611)
                     /gene="radA"
                     /locus_tag="IWK44_000231"
     CDS             complement(52241..53611)
                     /gene="radA"
                     /locus_tag="IWK44_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP9548789.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(53674..54201)
                     /locus_tag="IWK44_000232"
     CDS             complement(53674..54201)
                     /locus_tag="IWK44_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP9548790.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            54431..55237
                     /gene="proB"
                     /locus_tag="IWK44_000233"
     CDS             54431..55237
                     /gene="proB"
                     /locus_tag="IWK44_000233"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP9548791.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            55234..56481
                     /locus_tag="IWK44_000234"
     CDS             55234..56481
                     /locus_tag="IWK44_000234"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP9548792.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            56557..56901
                     /locus_tag="IWK44_000235"
     CDS             56557..56901
                     /locus_tag="IWK44_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548793.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(57018..57344)
                     /locus_tag="IWK44_000236"
     CDS             complement(57018..57344)
                     /locus_tag="IWK44_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548794.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(57959..59161)
                     /locus_tag="IWK44_000237"
     CDS             complement(57959..59161)
                     /locus_tag="IWK44_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9548795.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            59278..59793
                     /locus_tag="IWK44_000238"
     CDS             59278..59793
                     /locus_tag="IWK44_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9548796.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(59992..60438)
                     /locus_tag="IWK44_000239"
     CDS             complement(59992..60438)
                     /locus_tag="IWK44_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9548797.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(60485..61963)
                     /gene="msr"
                     /locus_tag="IWK44_000240"
     CDS             complement(60485..61963)
                     /gene="msr"
                     /locus_tag="IWK44_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9548798.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(62317..62946)
                     /locus_tag="IWK44_000241"
     CDS             complement(62317..62946)
                     /locus_tag="IWK44_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9548799.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(62930..63394)
                     /locus_tag="IWK44_000242"
     CDS             complement(62930..63394)
                     /locus_tag="IWK44_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9548800.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
BASE COUNT        17662 a        13670 c        11370 g        20717 t
ORIGIN      
        1 gttattcatt aaattggaat ctagattcta ttttttccgg aggcagtcat tctgatgctt
       61 tgaaccaacg gatgaagcaa ttggaagacc aaatgaacga gtattatcac cgcgtaacaa
      121 aatggtcacc tagttctgac aatgcagagc agttaaacgc tattcttcaa ttgcaagaaa
      181 cgattacgaa tggttttacc caatgcagca gctatattac tgcattgctt tctgccaatg
      241 tcaatgattc agatgcaaag gtcttatcag gcaagcttta cgcaatgctg cctcgtctgc
      301 aatcagccga aactgtattg tcaaagaaat ttgcagaaat atctgacaat gattggaatc
      361 agatgctttc tcaatcttcg tttgaaacaa ttgcttttcg tttaaacgag attcgtcgtg
      421 atggctcaca attattatca gaagctgaag aaaacattat aaatacgctt tctttagatg
      481 gtctaaatgc ttggagcagt cattatgata cgattgtagc cagcatttcg attcccttcg
      541 aacaagatgg acaagtagtc gaattatctg ctggacaagc cttcaataag atgatgggcg
      601 atccagatcc aaaagtcaga gaaacacttt tcgcagcatg ggaagaagcc tggaaagaaa
      661 aggcacccct cttcacggat actttgaatc atttagatgg ttttagactt tctgactatg
      721 aacttcatgg tgtcacagat tttctacaaa aaccattaga gtacaatcgg ttgaagaaag
      781 aaacattgtc tgtcatgtgg gatacaatcc agaaaaacaa acaacccctt gttaattact
      841 tgactagaaa agctaatctt ttcggtaaag aaaaaatgga atggcaagat caagatgcac
      901 ctattatttt aggcgatttg aaagaaaaaa cctttagttt tgacgaagca gcagcattta
      961 ttatcgaaaa ctttaacaaa ttcagtccaa aaatggctaa atttgcccaa tctgcttttg
     1021 aaaagagctg gatcgaagca gaagatcgtc ctgggaaacg tcctggtggt tattgtactg
     1081 agttaccaga aacaaaagaa tcaaggatct ttatgaccta cagtaattct gttaatgagg
     1141 ttgcgacact tgctcatgaa ctaggacatg ctttccatag cagcacgatg tgggatttac
     1201 cttctttaaa tcgcgaatat gccatgaatg tcgcagaaac agcaagtaca ttcgcagaac
     1261 tgatcgtagc cgatgctaca ttaaaagtcg caaaaacaaa agaagaaaaa atcaatttgc
     1321 tagatacaaa actgcaaaat gctttagcta tgttcatgaa tatccattcc cgattcattt
     1381 ttgaaaatcg attctatgaa gcacggcaag aaggattagt ctcagaagat aagatcacag
     1441 agatgatggt tgaggcgcaa aaagaaggct atcaaggagc tttagctact taccatccat
     1501 atttctgggc tgcaaaattg catttcttta ttgatgacgt accgttttat aacttcccat
     1561 acacatttgg ctacctcttt agtttgggca tctatgctta cgccaaccaa aagggagcaa
     1621 attttgaaga agaatatatc catttactac gcgatactgc ctcaatgacg acggaagatc
     1681 ttgcgaagaa acatttgaat gtcgatctta cccaaccaga tttttggcaa gcaggtatcg
     1741 acatggtgac tgaagatatc caaacgttca tggaattgac agaagaattt atctgatttc
     1801 tattttatca caataaaaaa agagttagtc cagaatgggc taactctttt tgattaatca
     1861 ttcttacatg ctgacagcaa agtcaggggc aaaccattga actgaaccaa cttttacgct
     1921 ttcgcctggt tgaggagcat ggatatattg tcctccgcct aaagcaatcg ctacgtggta
     1981 agttccgcct tgtgaccccc agaataacaa gtctccagct tttgcttgag aaactgaaat
     2041 ttttgttcca gctgattctt gaggaactgt ccaaccaccg atgtcacgac cagttacttg
     2101 catgtaaaca tagcgtgtga accctgagca gtcaaagcca cttggatctt taccgcccca
     2161 aacatatgga gtaccaatat atttgtaagc ttctgctacg attgctgcac cattcacact
     2221 tccagaagga gctggagcag gtgttacagg ttttggttgt tcaggtgttg gtgttggcgt
     2281 tggagccgga gtactacttc ctgtgccgtt tcctgtatca acactttgat ctgttgatgg
     2341 tgttgtaggc gcaggtgttg ttgattcttc tgttgttgaa gattcaggca ctgttgttga
     2401 ttcttctgtt gctgaagatt caggcactgt tgttgattct tctgttgctg aagattcagg
     2461 cgctgttgtt gattcttctg ttgctgaaga ttcaggcgct gttgattctt ctgttgaaga
     2521 ttcaggtgtt gttgattctt ctgttgctga agattgtgta gctgatgatg attcttcagt
     2581 cattgaagat tgtgtagctg atgattcttc tgttgctgag ctttgtgcag tagatgaagc
     2641 ttgagtagct tgtgcttcag cctctgcttg ttcgcgtgct tctttttcag ctttttcttg
     2701 tgcagcttgt tgacgagctt gttctgctag acgttgttgt tcacgaatac gagcttgctc
     2761 tgcttcagct tctgcttttt gacggttcaa gtcagctttc ttgtcttcag cagttgcttg
     2821 ttcagcagct aatgaagttt tcagtacgtt caaatcagct tgtttagata aaagatcgcc
     2881 tttttgagat tccaaagcag cttggttttc agctaattct tttagtttag catcgttttc
     2941 agcttttttg tcttctacag cttttttatc ttgtttttgt tgttccatca agtcgttgtt
     3001 tgcttttacc atcgttgtca ttgcttgaac gcgtccaata gcatctgcta atgaatcagc
     3061 attcaataca gcatcgatgt agtttgagct tgtgttgctt acttgtgctt cgcgtgcttg
     3121 tttttggatt gtatcttcac gtttttcaat acgttcttgt aaatctgcaa tgtcttttac
     3181 taattgtgca gattcttgac gcaatgtatc ttgttttgct aataggtctt gtgcttgtgt
     3241 gttgatttca gaaacttggc tttctaaggc atcgatttgt gattgtgcat ctgcttgttg
     3301 atttttcaag tctgcaatct tttgatcttg ctgttgtatc tgagaatcaa aatcgtccgc
     3361 cgcggcagcg atcggtgatg ctacggcagt caaggtcatt gaacatacca ttactgctga
     3421 tattaaactc tttttcactc ttcattcctc cgactggctt aatttaataa atatctaaat
     3481 atatttaagt ctttcttaac gatataaaaa ttgtaccata gcagtgtgtc aacgatataa
     3541 tagttatgtt acaaaaatgt ttcaaaacaa cttttaaaaa tgacaaaact gttcgtttca
     3601 gaaaatgatc atgaaaaagc cgcgttaagt ctagcatttc ttaagataac aacgattctt
     3661 tttcgctttc gacctaaaaa taaaaaaaga aattattcat cactgaataa tttcttaaag
     3721 ggaaaaatga ataatacaaa tataatcatg ttcaacagca gtgtaggtcc taaaaacctt
     3781 gtaataaaat acgtgttatt tactactgct aatttaaaaa tcaactggac caccatcgta
     3841 aacaattcat aacccgtgac aaagatgatc atactgaaaa attcagtaaa gatgttgaca
     3901 tggataacgt ctttcattac atacatcaac catacaatta aagggagagc tactgcatag
     3961 ataccgataa cgccaatata gtaagcgtca tagatagccc cgagaacaat cgtggtaatc
     4021 agtaaataac gtttttcaaa cgccatactg caacataata atgccaatat cagaaagtgg
     4081 gcatttgaca tataggtgcc tttcgaccac tcccctagca ttcttgttaa atgaccatct
     4141 atcaacatca aaaagaacaa aacgattggc agatagtact tcattgtttc tttcttcaac
     4201 atggactaat cctccgctag ccgttggaca atcgtcacaa ctgacacgtc atacatttct
     4261 gcataaggtt tcacataaac ttcccgatcc agcccataac tatctggttt cgttttaata
     4321 accgtaccga tcggcaagtt agctggagaa ttccctccaa gaccggatgt ctggacaaca
     4381 tccccttctt tgatatccgt atcaccagtt aattgggtca ccactaaggc atggagcttt
     4441 tcatcatagt ttttaagcaa accaaacgct tcgccattag ccgaagatac ccgtactgga
     4501 aaatgattgg agctttcatt agaggatgac agtaattcga ttttagacga agccgtattg
     4561 acctcgatca ctcggccaat caatcctttt tgcgacatga ctgccatatt tgcttcgata
     4621 ccgtctttag aacccttgtc tacgatcagc atgtcctgcc aactgtcagg cgaacgagta
     4681 atgacatttg ccgttacttt ttcgtaacta gtcagcgtct gattcaactc aagttctttt
     4741 ttcaacgctt cgatttctct ctcgtaattc ttgttttgct gtgaaacgtc atcatagcta
     4801 tcgatttttt cttttagccg ctcattttcg gcataagttg aaaaaagatt atggacagaa
     4861 tctactccat ttcgtaccca ccgtactggg gcagagattg ttttatcaac cattgcaaca
     4921 ctgtcattaa cgatggattg gaagaaattg ggctttcctt cattcgctcg ctgcgcagcg
     4981 gtcacactta gtatcgtcac cacgatgata acaataacca aggtaataat aatattttta
     5041 ttaggattga acttcttcac aaagacacct cgaattaatt agactcttgt gttcaatttt
     5101 atcactaaat gccgtgaaac aaaaggtaag ttgctcctct taagcttttt tttagcaaat
     5161 aatagattct gtcattttta gcctgaaaga caaatttttt tatttgttta tggatcaatc
     5221 tttgctttga ataactgcaa tcataccatc tgaaaaagaa aattctgcgg tatctgaaag
     5281 aaattcgtta cttgcataag atgtcggata ttctacttct ttattttcca atacatactt
     5341 gctgcctttt aatgtaagat tcgagacggg agtcaagcag caataagcta aatacttcat
     5401 atctggtatc tgtcttacta tatgctctcc aggcaaataa taagacaaat aattttgaca
     5461 atctctgata ataagctgat gggcaaaggg tttgaagcga ggttccatac ctaaccagag
     5521 attggctaaa agatgatcca atcttccgcc cgtagctcca atcaaagtga cttccgcttg
     5581 cgggtaacgt tcaaatgttt ttaagatacc taactgtgta tctgtatcgt ctttttctgc
     5641 aggggaacgg gtatactctt tggcttttgc aaatacttcc ttctgctctt cgttcgacaa
     5701 tgaatcaaaa tcacccactg ccagatctac tgggtagcct gcttgaagca ggtacagcgc
     5761 accacggtct atcccgacat aggcatcgta tttggacagc agagactgcg gccagttttg
     5821 cggattacct ccagcagcta ataaaatttt catttagtct aatgcctctt ttaatcgttc
     5881 gatttgtccg actggatttt cggctccata gatatatgat cctgcaacga agacatccgc
     5941 ccctgcatct ttacagattt ttgctgtttc tggaacgatt ccgccatcta cttcgattgc
     6001 ataagtccaa ttgttctctc tacgtagatc agcgagttcc gtaatttttt ctactgtttc
     6061 ttcaataaag ctttgtccgc cgaacccagg attgacagtc atgactagta cttgatcagc
     6121 caaaggcaaa acatgtttga tcatggagac tggtgttcct ggattgataa ctactccagc
     6181 tttaactcct aagctttttg tcatttgaag tgcccgatga atatgaggag ttgcttcgac
     6241 gtggacagta atgatatctg ctccagcttt tgcaaatgct ggaatgtagt tttctggatc
     6301 aacgatcatc agatggacgt ctaacggtaa tttagtaaca ggacggattg cctgaacaat
     6361 atttggccct aatgtgatat ttgggacaaa tcggccatcc attacatcta cgtggatgta
     6421 atctgcgcct cctttttcca ctaactcaat gtctctttgt aaattggcaa aatctgcact
     6481 caaaatggat ggtgctatct tcattcttgt ttcctcctat tttcgtttac cttctttttt
     6541 tgcatacatt ggttttcttt tttcgatttc ttgtaagaat tgcaaataat tgtcgtaacg
     6601 tgtctgtgcg ataccacctg tttctactct atgttttact tcgcagccag gttctttagc
     6661 atgcatgcac tctctaaatt tgcaatggac agaagcttct acaaattcag gaaattgtct
     6721 cggcagttct cctgcttcaa ttgtcaaaaa gtcaatcgag ctaaatcctg gtgtatctgc
     6781 tactaacccg ccatagatag gaatcagttc cacatgacgt gttgtatgcc gtcctcttcc
     6841 aagtgcatca gaaatctctc ctgttgctaa attcagttcc ggtgatattt gattcagtag
     6901 cgtcgatttt cctgctccag actgacccat aaagaccgat agccgttctg ggaagtaacg
     6961 gatcagttct gtcgtatcac ccgtatgatt gggtacaatc accggatagc ctattttagt
     7021 ataggtttgc ttgatctctt cgattttttc tgggtcgtct gacaagtcgg tctttgtcaa
     7081 aaaaataatc ggttcgatcg actcgtattc caaggtaact agaaagcggt caagtagatt
     7141 ataagaaaat gcaggctcta cgatacttgt gacaatgatt ccaagatcaa catttgtgac
     7201 tggtgggcga actaattgat tttttctcgg caagatttcc agaagatagc cgtctgtttg
     7261 attttcactc tcaaagatca cctgatctcc gacaagcggc gtgattttcc gattccggaa
     7321 atttcctctt cctcttgttt gataggtctc tccgtttgcg tatacatagt aaaaaccgct
     7381 gattgctttt ctgatttgtc ctttgataag tgccacctcc gtttttgttt attttaccat
     7441 acgaagagga aagtaggtgt tattccatgc aaacttctca aagtatatgg cttgaaaatt
     7501 gatcaaaaga gatagaaaca caaaaaagag aatgcaacaa atcaatgtcg cattcccttc
     7561 tgatatattt tattcagcac ttattttgaa ctttctgagg tgctagctga tgaacttgtg
     7621 gagctggaag attcttcttt tgaagagttg cttgtgctac tggagctact tgagctgcta
     7681 gaactgtctg aagagtcttt ttcagaacta gaagacgctg gatctggtcc tttagagtaa
     7741 attacgttca caaccattcc tttggtaatt gtcgttcctg cccctggtac tgtacgaatc
     7801 actttatctt tatccaccgt gtttgaatat tcggaagtct catgcccgat atattctgca
     7861 ccgacactag ctaaataact ttgtgcttcg ctttttgtat agccggagat atcattcaat
     7921 ttaggctctg ttccttggct tacgacaaaa ttgatgtaag tttcttctgc tacaacttca
     7981 cccgaggctg gatcctgact gatgatcgaa cctgatggta cagtgtcgct atagtcataa
     8041 gtagcactaa tattattgat attgaatcct tgtgtaacaa gccaattacg aacagtttcg
     8101 tagttttctc ctacatgact actcaaagtc actgtttctg gtcctttgct gactgtgaaa
     8161 gtaatcgtgt ctgttttagg atcgacttct gtatcttctg atggagattg cgaaatgatc
     8221 attccgcttt ctacctctga actgaattcc tcttcttttt gaatcaagtc agcagaaaac
     8281 cctaattctt tcaaacttgc ttcggcatct tcataacttt taccactgta atcatctaat
     8341 ttgatttttt tcgtgccgct actaatatac agagtgactt cgcgtttttg cttgactgtc
     8401 gttcccgctg ccgggtctgt cttcactaca ttaccttctt cgattttgtc atctgcaatt
     8461 tcttctgttt ttgttgctac ttctaatttc gcttttgcga gtgtttctct agcagaagct
     8521 tcagaaagat cagaaacatc aggaacaaca acttctcctc gcccgccaga agcaaagaat
     8581 acagctgttc cgattcctaa aacagcaaga atagctaata ggatcagcca ccattttttc
     8641 tgtcctttct ttttaggatc aggcagaact tcttcctcct ctggtaaggt ttcgttttct
     8701 ggcttgtctt ttggcagagg catagatttg aatgaatcag gcattggtgc atcttccgta
     8761 aggggtgtga taacttttgt ttcattatcc atcacatgag gctgccattt ttcttcgttt
     8821 gcacgagctg gtgataatac tgtagaaaga tcatcagaca tttcctcggc tgacttataa
     8881 cggtctgcag gttctttagc cgttgcccgt aaaatcacgt tttccagtgc ttgtggcaca
     8941 tttggatcaa gtgcttttat agatggaaga tcatcttgaa aatgttttaa agcaattgtt
     9001 acagcagatt caccatcaaa cggtacgcta cccgtcaaca tctcataaag gatgatccct
     9061 aaagcataaa tatctgactg cttcgttgcc atacttcctc tggcctgctc aggggacaga
     9121 taatgaacag atcctagcat cgtattcgtc tgcgtgatcg atgtttcaga taaagcgata
     9181 gcaatcccga aatccgtgat ttttacaaca ccttcattat cgatcagtac gttttggggt
     9241 ttcaaatctc ggtggatgat ttgatggtta tgagctaaag aaatagcaga aaggatctgc
     9301 tgcataatat ttactgctgt ttcataagga acaggataat gtgtttggat atatcgtttc
     9361 aagtccattc ctttgacgta ttccatcacc agatactgca tgttgtcttc ttcaccaaca
     9421 tcgtaaacac taacaatgtt tggatgaacg agttctgttg ctgctaaagc ttctctctga
     9481 aatcgtcgaa tcgcagtctg gtcattttga aagtcaaatc gtaagacttt aactgctaca
     9541 tcacgatcca agattaaatc gtgcgccaaa aatacatttg ccataccacc gctaccgata
     9601 tttccagtga tttgataacg gccatttaat tttttgccca actcgatcat acactgtcct
     9661 ccttatattc gataagaaga acggtgatat tatccgctcc gcctgcttca ttggcacgat
     9721 caatcagctc agttacttta tgagacaatg ggccttcttg attgatgatc gttcctattt
     9781 cttcttcaga cagcatgttc gtcaagccat ctgaacataa cagcagtcga tcttttagtt
     9841 gccaaagatg agtagaaaca tctacttcga ctgttccagg catgccgaca gaacgtgtta
     9901 aaatattttt ccttggatgg ttcactgcca tttcttcgct gatttctcct gatttgacta
     9961 gttcattgac taaagaatga tcctccgtca attgcatgat tttatcttca cgaatcaaat
    10021 aagctctgct atccccaaca ttggcaatcg tgaaccagtc atctgacaga gcggccgcta
    10081 cgattgttgt tcccataccg ttatattccg gctgttcttg tccacgctga taaatcagca
    10141 tattttcttc ttgaatcgat tgaatgaacc attgggcgat tttttcatcg tcaacagctt
    10201 cttgtttttc ccaagcttcg cctagattcg ttaccgccat ctgactggca atgtctccgg
    10261 cacgatgacc acccatcccg tctgctaata cagcaagctt gagacctgaa cggctggtaa
    10321 atacactggc atagtcttga tttgtgttac gtcgtcttcc tacatctgat tgatattcaa
    10381 tctgcatttt ttcacctcat tactttttgc gcaaacaaca aatgaaaaat ccatctgtgt
    10441 aaaactgatg cggatacaaa gtcaacattt tctcttcgat tgatgattgt agcataggat
    10501 tggtcaaaac atcaatcctc tcaaaatcag ggtgtctttc caagaaagca gaaacaacgt
    10561 cttggttttc ttcttttaag atcgtacaag tgctataagt cagaataccg gaagacttta
    10621 aagttggtgc gcagctttct aaaatttcca attggatttt tggtaaattc gctaattcat
    10681 ccggctgttt tttataacga atatctggtt ttcgccgaag cagcccaaga cctgaacaag
    10741 gcgcgtctac taaaatccga tcaaactgct ctgcttcgaa ctgtttactg acttctctag
    10801 catccatctt ttgagcgtag acagcagaag agacacctag acgtgctgca ttatcttcaa
    10861 tgagtttgac tttgtgttcg tgtacatcta aagcgaccac ttctccaccg catgacggat
    10921 ctaaaaaaga agcaatatgc gtcgtcttac ctccgggtgc tgcacaggca tctaagacat
    10981 gatgttcagg ctcgatttgc atagacggcg ctacaagcat cgaactttca tcttggatcg
    11041 tcatgacgcc atcgtggaac aatgagctag aagctaaatg cccttttttc gctactatac
    11101 cgtatggact gacttggctt ttttcagctg tgatctcttc ttcttgcaaa acagtgatgg
    11161 cctgatctct ggaaagtcgg cgtgtatcta ctcgggcact agcatggcta ggctgatata
    11221 gcgataaacc gagcttttca gtttcttcgt atccgatttg atccaccagt cgtttcgtca
    11281 tccatagagg caggctgatt ttgacagaaa gtctttcgat aggatcagca atgccttgca
    11341 gatctggtgc tccttgacgc tgataattcc tcaagacacc atttacaaat ttccctgtcc
    11401 caggatttcc ttttactttt gcgatttcga ccgcctcgtt taaaatagca tgtgcaggga
    11461 ctttatctaa aaattctaat tggtatacag aaagataaag aagcatcttg acccagtcat
    11521 ccactttttt tgctttagcg atcaatggtg caacgtaaaa ttccaatagt cgctgatggc
    11581 taatcgtacc atagatcaat tctgtaaata aacccacatc tttttgattt agctcgcttt
    11641 gtttgatcat ctcattcaac aaaagattcg agtaggcgcc acccttgttc actcgctcta
    11701 atgtcagtaa tgccatatat cgtactgatt ttttcatttt tttagggatt tttttcatta
    11761 tttcacctgc tgaccaatct gaacttgctg accgcttccg ttaaggaaat catgtacggc
    11821 ctgttttcct ttaccagctg gctgtaattc attgatggct aagacggtct gtttaccgca
    11881 agcaatccat agattttttt tgtctttccg gatgatcgta ccaggtgctt tgtctgtagt
    11941 ttctgccaat acttcaaccg ataataattt ccagcgtatg ccctcatagg tcgtgaatgc
    12001 aattggccaa ggacgcattc cgcgtacttg gttgtcaatt tcttctgcag ttttatgcca
    12061 atcgattgct tcttgttccc gtgtgatgtt tggcgaaaaa gttgcttctg actcgtcttg
    12121 aggtactggt ttttgtccat ccaaaatatt gggcaatgtt tctagcaata gctttcttcc
    12181 taaaaggcta agcttttcaa acatcgtccc tacatcatct tgtttagtga ttggtatgct
    12241 ttcttgcgca tagattccgc ctgcatccat ttttttgatc atttccatga tcgttacacc
    12301 agtttctttc tccccattca tgatggcata atgaacaggc gcacctcccc gatatttagg
    12361 aagtaaagaa gcatggacat tgattgcccc taattttggt acttgcaata acttttcagg
    12421 caagaattgg ccaaatgccg cagtgacgat cacatcaggc accaattcaa tgatttcttc
    12481 catttctggc gaaccggaga ttttctctgg ctgcagcaca cggataccgt gcttcagagc
    12541 cgcttttttt accggtgtcg gcgtaattac tcgtttccgc ccaactggac ggtcgggctg
    12601 tgttaccact gcctggatct cgtaacctga ttctatcagc ccttcaagaa tcggcacaga
    12661 aaaagcaggt gtgcccataa atactagctt agtcatcaag atgttcctcc atatattctt
    12721 caagttcttc ttccgggatc tgctgtatca ttttctcaat gaacaagata ccgtctaagt
    12781 gatcgatttc atgctggaag gcacgggcta aatagccaaa agccgtcact tctatctctt
    12841 ctccgtctcg atcataataa cgcacggtca cttcatctgc tcgcttgacc gttccataga
    12901 catgcggaat actgagacaa ccttctacgt caaggctttc accttttgct tcgatgattt
    12961 ctgggttaat cagttcaaat ttctcgcctt catctacttc gatcacagca attctcttgt
    13021 tttgtcctac ttgaggagca gcgatcccta ttccgtcatt cgcaatcatt gtctcatata
    13081 aattgtctaa taaagcgatt gtctcgtccg tgattacatc gattggctga gcagttcttt
    13141 ttaatttatc attagggtgg atcaatatag gatagcgcat aatttggccc tttcttaaat
    13201 aaagtttaat ggttcgttgt caattgccac aaaaagacct tgtcgctgtt ctttctggct
    13261 tccattcaaa atttcatcca aaacatgagg aagattcggt tcatgtttat atttgatgat
    13321 tagttgataa taatatcggt ttttcactct caaaatcatg ctgggcgttg gccctaatag
    13381 caagctttga gggcttaaca cttcttttag ctgattggca atctgaaaga ttttctttgc
    13441 cgctacttgt tcttcaggat ggctggcggt tattttcaca gtaaagtaat aaggaggata
    13501 gcctccacga tgccgtacat gcatttcctg ctgataaaac tgctcataat tttgtttttt
    13561 agccaattct atggcgtaat gttgaggatt gaatgtttgg atcacgactt cgcctgcttt
    13621 ttccgctcgc cctgctcgtc ctgaaacttg ggtaagcaat tggaatgtat gctcactaga
    13681 acgaaaatcc ggtaaattca gggaggtatc agcattcaac accccaacta atgtcacttc
    13741 tggaaagtcc aaacctttag caatcatttg tgtccctaat aatatatctg cttcttttgc
    13801 accgaatttt tgtaaaatct gttcgtgtgc cccttttctt cgagtggtat ctacatccat
    13861 acgcaaaatc ctagcttgcg gataaagttc tcggagttcc tcttctactt tttgagtacc
    13921 tgttccatag tagcggattt tattgccgcc acaattagga cagcgattgg gaataggttc
    13981 ttcatgtccg caataatggc agcgcattga acgtgtatcc atatgcagtg taagagagat
    14041 atcgcaatta ggacatggaa gaacaaatcc acagtcccta cacatcacaa aagacgaata
    14101 tcctcgacga ttgagcagta ggacagtttg ttcttttttg tctaatcgat tttgtatttt
    14161 ttcctgcaaa ttagcagaaa aagtagaggt ccgatgattc tgaaactctt cacgcatatc
    14221 tacgatctca atagctggaa gctgtgcagc tgctttggct cggtggttta actgcaataa
    14281 ctgataaacg cctttttgag ctcgtgctct tgattctaaa gagggcgtcg cgcttcctag
    14341 aactattggg caatgatgat atttactgcg ccaaattgct agatctctcg catgataacg
    14401 cggtgtctca tcttgtttgt aacttgattc atgttcttca tcgataatga tcagcccgat
    14461 atttttcagc ggggcaaaaa tagcagatcg tgcgcctact actacttgcg cttcttcccg
    14521 ctcaatcttg cgccattcat catatttttc accttgggaa agtccgctat gtaagaccgc
    14581 aactgattct ccgaagcgtc ctttgaatcg ctcgaccatt tgcggagtca atgcgatttc
    14641 aggcactaac ataatagccg ttttattttc tgacaaaaca tcagcgattg cctgaagata
    14701 tacttctgtt ttcccgctgc cagtgattcc ctccagcaaa aagacctgat ctttttgctg
    14761 ctgacctgct gtgatgattt gttcgacagc atttttttgt tctgcattca gttccaatgc
    14821 cgtcgtttgg tcaaatactc ggtctttata agggtcgcga taacgttctg attctataaa
    14881 ctccagccag cccttttctt ttccttgatt caagacagca gtactgattc ctttattttt
    14941 gaaataagca attggttctt cttggctcag ttcagataaa cacaaaagaa gttctttttg
    15001 tttcttggca ttttgccgta attttgccca ttcttcttcg atttgctttt cagttaacaa
    15061 agaacggacc aaacgaatcg tttttacttt atttcgtgta tggacttcat atttgatatc
    15121 tactaattgc tcttggcgcc agcgaatcaa ttgagaaagg ctgccttctt cttctgcttc
    15181 cttccaggaa atcacttctc gctgatcaaa caatacctga acttctggtg tgtctgccaa
    15241 aggatagatc aatttatcat attctgcttt catcacactc ggaagcatcg tttgtagaca
    15301 agtgattttg aatgcgtaag taattttttt catataatca gctaatgaaa gtaattcttc
    15361 attgaccacc ggagcaaggt ctagcaaacg aataactgcc ttcaaagatg gattagctga
    15421 ttcttctgat ttttggatag ccataacaaa tccttggaca tgacgattgc cattaccaaa
    15481 agggacttct actcgcatcc cgacttgcaa agctgtttcc atttcagagg gaaccaaata
    15541 agtgaatggc tgatcggtct gcatagtagg tacgtccaca atgacttcag ctgttaatgt
    15601 catggttcgt ttcctccttt ttttgtcttt tcttatcaag cgacaaaaag ttccttgcct
    15661 attttactag agatcgatgg atttgtctta tcttctgcca aatcctaaca aaaaattaac
    15721 agcagataag aaaaaaaaga actgtgtcaa aaagttcaga cacagccttt tctaccagac
    15781 aataagtggc gaatgtttga aaaggaaaca gctgggtttg cttttctttt tagatgttcg
    15841 ctctattttt ctttattaca ttttttgctc ttcacggata cgtgattcca attcgtgttg
    15901 ttcacgctct ttttgtgctt gacgttcctc ttcttccatc ttcaaacgtt cacgttttaa
    15961 ttcaggatgt ggatcattga ctacatttcc tgcatcgatt tcttctaaag cttgaccgac
    16021 gctttttact gaatcaaaag aatctagagt aggctgtgca cttgcatcca attcatgcgc
    16081 gcgtttactt gctaaaatca ctagagaata ttttgaattt acacgatcta atagtgagtc
    16141 aattgaaggt tttaacatca taagactaca tctcctttaa cattttaata tatttaccga
    16201 ttacgcgatc tactcgaaaa tgttcactgg caataatgtt tttgattcgt tcaactgcta
    16261 gcggcacttc atcatttaca acagcatagt cgtaaagagc catcatttcg atttcttctt
    16321 ttgcaacttt cattcgtttt tcgattactt catctgcatc tgttcctcgg cctacgatcc
    16381 gtgattttaa ttccgctaaa tctggcggag tcaaaaagat gaatacacca tcgggcactt
    16441 tttctttgac ttgttttgct ccttggactt caatctccaa gaaaacatct tttccttcat
    16501 ccaatgtctt gttgacatat gacaaaggag ttccataata attcccgaca tattctgcgt
    16561 attccagcat ctctccagct tcgatcattg attcaaactc ttctttgctt cggaagaaat
    16621 aatcaacgcc ttcgacctcc ccttcacgca ttttacgggt agtcattgag atcgaatact
    16681 gaaaatcatt atcctcactc tcaaaaattg cttttcgtac tgtgcctttt cctacaccgg
    16741 atggaccaga caacacgatt aataatccac gctctgacat gacgacgtcc tttcaactag
    16801 tctttttgta cttatcgttt attttgcact ttttttctgc aactttcaag gttatcttta
    16861 attatagcta gaaattggta aaaaagctac cctgtaccaa gaggtcgtaa aaaagctgtc
    16921 ctgcatcaag taataagaac agccaaacat aaacagcttc tcatgttttt cgtttggttg
    16981 gggttattat cgcagatgca agcttttgaa cgccgtgtat aagaggtcgt gaagaagccg
    17041 attagtttcg agtatgaaga aaaattttgt gaaaatagtt tctcacattt ttgcataaat
    17101 ttggcttaat accgagaaaa tggtttttga acatcactta ttcaaaataa aaaaagattg
    17161 tgacacgaat tttgtcacaa cctttaaatg tattcctaga gcaaagaagc taagcgaaga
    17221 aatccaattc tgtttattta ttgtatttct ttgttcagca tccttgctaa aagcccatat
    17281 tggacaaagt attcactaca cccatgatct catcttttcg agaacgtgct ttttctgtcg
    17341 gatcttctac ctcttctgtg tcaattcgaa caataaattt ccgattcatc gtcacgtaag
    17401 caccgtcgtt gatcaaaata aacagttcgt tctcattata gtctgcacag gcatctttcc
    17461 atacgtcaaa gccgtttttt tcagaaggga tatcccgttt aaccacagtt tttccactta
    17521 attcgtggaa cgttacgtca taatatttca ttatctctac ctctttcgtc attcaatatt
    17581 ttgtacttgt tctctgattt tttctaagat cgtcttagac tggacgactt gatttttaat
    17641 ttcgattgca tttgatttgg aaccgatcgt gttgatttcc cgattcattt cttggatcag
    17701 gaaatctaat tcccgaccta caggacattg tgcttcgata agttcatcta gtttttgtac
    17761 atggatcgcc agacgatcca gttcctcgtg aatatctccg cgttccaaca agatggctag
    17821 ctccgtcaac aaccgttgct catcgacttt cgctccaaga aattcctcca gctttttttg
    17881 ataccttatt tgaaattcct tttcatagat ttctacaaaa ctatcgagtt tttttagtac
    17941 ttctttgaat tcttcagtat tctttttcaa cactaaagct aaagcttgac cttctttttc
    18001 tcgactcgcc tcaatagaat caagtgcgct tttcaatgta tctagagcta actgtgccaa
    18061 ttcttccatg ctctcttccg gtttttcttc taccgttacg aagttttcgt ccagcacgaa
    18121 gcgttccgcc atccgactaa ttgacacttg attttgacta ccgtatcgtt gttgaattcc
    18181 gtctgtcaat tgagtgataa gtgaatcaat caattcccag ttgatgacga tctgtttttc
    18241 gtttttgcca atataagcta gattcacaaa tacttcgatt cgaccgcggc ttaactgctg
    18301 tttaataagc tgacgaaaat cgttttcaac aaagttcagg attttaggac ttcgtatttg
    18361 aacatctaga aaacgctggt tgacactttt tatttcaata tctaactgat acgcttttgt
    18421 ttcgcggatg gctttgccaa aaccagtcat acttttcatt cgacagggtc cttcctcatt
    18481 acatattttc tattgctttt tgcagttgtt caaactcttc gttcgtcaat gtcaggcctt
    18541 tccccatttt ttcatggtct ggcgcccaat ctcgaagatc gaattttggc gggcgaccat
    18601 tccagctgac taggttcagt tctttacgcc accccttgtt gttttctgac aatatagcga
    18661 tttcttctac gatttcatat gaaaattctt gtgccatggt tgttcctcct tgagataata
    18721 taaatatagg atcagtaatg tgtcaactgg ttgcttaagc aattcatgct gcaaacgatc
    18781 cttgcgtttt ccttgtagca gataatcaaa ataaacagtt ttcgtgcgct cattttgtat
    18841 tttttgctgg tattttcgat accttcgcca ttcttcagta cttaattgac caatttgtgc
    18901 caataaatca tccggcatac tttttatatc ttgttggcag atcatttgaa tcatcatgtc
    18961 tacttgatag atttcattgg gcagtaaaaa atgttttttt gctaaaagtt ctagcatcgc
    19021 caatccgcat gtctctggta cgaccctgtt cttatataaa ttacgcagtt tttgccaaaa
    19081 ttttatgctt ttgaaaaaag ataattctaa ctgaatttct tttgttaagt aagacaaaaa
    19141 tcctcgccag tatgttcttg caggtttaac agaatgaaat gtataccagt tcccatagta
    19201 atagcctaat cgttttttca tttccagata gcctaattgt aaatttaatt ggttcctttg
    19261 gctatcaaaa agcagaaagc tgcctagtgt ccacaatgtt tgacatttcc aatggataaa
    19321 aatgtctggc atacgaattt ttttcgctgg gcctggccct tgcacatcta cgacaaatgc
    19381 ctctgttgct cctttgttaa tcgcaatatc aataggtatt ttgttgcgat acccaccgtc
    19441 tgcatattta ttttttccga tctttcgata agccatagcg ggataaaatg aagcagaggc
    19501 gagtatccaa tctggtattt cttctgtttt tgtctgttgg atatcagtca ctacttccgc
    19561 tagttctggc agtttagtag acaccgtata taaacgtatt ttgggattat gtttgagtaa
    19621 ggacaaatcc aaattgtctt tgaccatctt ttccaatgga gcagaagaaa ccccgccttc
    19681 aattagtgct gttttcgtca tttggcgtct ttctcgatag aagcgttcac gaaggtcttc
    19741 aactgcagcc atttctggta acgcaagcac ttgtcttgtc gacagctttt tccataaatt
    19801 cacagctttt ttcatatctc cttgtaaaat caatgcgccg ttcaacgcac ctaccgaggt
    19861 acctgtgata atctcaaagt tgatatcatg ttccttcagc gcctgccaca caccaatctg
    19921 ataagcaccg tgcgctcctc ctccacctaa aacaagtgct gtatgataga ccagcttttt
    19981 atttaattgt gtggttcctt gatagccgtt tttatgaaaa atacgcaacc attcttctga
    20041 tagaggagat tgaaataaaa aacaacagtt ttctttaata aaaaaacgag cgcaagcttc
    20101 catataccga aaaaattttt tccattcaaa agtttcacct tcaatcaaca gattattaaa
    20161 aataacatct cctctacttt tagcaatagt aaaatagacg gtatattttt tcgatactgc
    20221 tttaaaagca cattgctttt ttgcagaacg ccaaaaagcg tctctacttt cttgatgact
    20281 ttgaaaaaat ggaaggggtt gttttgcttg ctggataggc gaatacagcg acattttctg
    20341 tgaaaagtaa acttttttta tctccatcgc gctctccttt ttcttcaagt ataccagtac
    20401 gtcataaaaa ggtcatgagt ctcgtattcg actttgtaga ttccataaga aaacaattca
    20461 taaaacctcc tttaaataaa aaaatacaaa aatcacaaaa attaattgaa aatgagagtg
    20521 ttttcaatga aaacgtgatt ttgtaatgga attttaaaat aaaagaaaca agctttttta
    20581 atgcaaatgt tgtaaaataa aagagattaa gaggaaataa gaggaggaac atgatgactg
    20641 agtcagttta tgtacatatt gataccacaa gtaatgctgt tttaacaaaa gggctgactg
    20701 ctactgactt tgcaaacagt atcgtgcatt ttcctcaaaa cttgctcttg cttgatccat
    20761 ctgcttctgc tggagaatat gaatcccata cagggctgaa agtaatccgt ggtacagaaa
    20821 atattcagcg tttcttttcc tcttctcgta acaggcgtgg attcgatgaa ttgaagtgga
    20881 tcgactttac tgatttaacg atgctgaaag aattgacacc tttagaaatt tcagaattat
    20941 tatattttgg tcatatgaaa acacatcttc attcgccgtt tttttataaa ctacagaata
    21001 attttgtata cttcgatctt aatgatcagc tgaatcggat ttattatcgc tatttagatg
    21061 aattttatag gatttttgct agtaaattaa gttcgatctt gtcagataag atcaatgtaa
    21121 aaaaatcttt tttcaaaaaa gaaacacctg ttgaaaaaat cagtgtggaa ttattgaaag
    21181 aaatgcgtaa tttgatgcag gaagggatca ttttttcttt tgatcaagtt ggtttgatcg
    21241 atggtaaata tcgtatccca atctatgtga ttgaagatta tatttggaaa ttaaaaggca
    21301 cccggtatcg agaagaggaa atcatcgcta ctttaatcta ctctgtcacc gatcgacact
    21361 ggcaattggt agaagatgcc gatctttctt atctgccatt gaagtagatc tatttcttat
    21421 caaaaaataa aaaggaaaca agaaactagg acatgattct gtctcaagtt tcttgcttct
    21481 tttttattta cagctcctgt cgtttatctg gcacaaaaat cgaaggagtc gtttgttgga
    21541 tccctaataa aacgatgatc gttacaatag ctgtactcaa cccgctggca ccattcatta
    21601 cgaaagaaaa gagccaagga ttcatccccc atagtgcata cgctccccag aaaaccactc
    21661 ctgcaataaa atgccaaaaa tatctggaaa gtgcgccaat caatgcacca aacacgatgc
    21721 tttttctaat ttgtttcgta ttatcgtcta catagccttt ttggacattt gaagccatga
    21781 gccctgctgc gcctgcaaaa gtataggcaa tcgggtactc aatcaacact tgaacgacac
    21841 ttaaataata aacttgtccc aacggaaaat gtaggatgcc ccaaatgaaa cctgccagca
    21901 tccctgcctt tgtccctctt cttaacgcaa aaagagtcaa tggaatctgc cctaatgaaa
    21961 tagaaaaact gctgccgatc tggatcggaa tcaacgacaa caccatcgct aacgctgcaa
    22021 caatcgttcc ttctacccaa actctatttt ttcccataaa aaaacttcct cctttgaata
    22081 aaaaagtcac aaaggaggaa gcagctttcg aaaatagtca aacagctgtt tattcacaat
    22141 tcctacgctc gcataaccga tacaggtacg aagggtttag aaatagattc aatctcagcc
    22201 caattggact cccctttgtg agtttattta ttatttattt tttgtttcaa acaatggact
    22261 aactggtttg ttttcatgga tacgtttgat tgcatctccc attagtccgc caacacttac
    22321 ttcatcgatt ttgtcgattt tacgatcttc tggaagatag atcgagtctg taacaactag
    22381 acgctcgata gctgaatctt ctattctttg taaagcaggg ccggataata ctggatgtgt
    22441 acatgaagca tacacactag ttgcaccagc ttctttcaaa gcattggcag ccaatgtgat
    22501 cgtacctgct gtatcgatca tatcatcgat cagtacacaa gttttgcctt tgacttgtcc
    22561 aatgatattc attacttctg caacatttgc ttttggacgg cgtttgtcta tgatcgcaat
    22621 cggagatttc aaaaattccg ctaatttacg tgcccgtgtg acaccaccat ggtctggtga
    22681 aacaacgaca acatcatcgc ctttgattcc tttttctaag aaataattgg caataagtgg
    22741 cgcacccatc aaatgatcga ctggaatatc aaagaaacct tggatttgga ctgcatgaag
    22801 gtccaaagtc agcattcttg ttgcaccagc tttttgaatc atgtttgcta ctaatttcgc
    22861 tgtgattggt tcccgtgcac gtgctttacg gtcttgacgc gcatagccat aataaggcat
    22921 gactacgttg attgtttttg cacttgctcg tttcagtgcg tcaatcatga tcagcaactc
    22981 catcaagtta tcattgactg ggctgctcgt tgattgaata acatagacat gcgatccacg
    23041 aatactttct tcaatgttta cttgaatttc tccatcacta aattgagtaa cagatgattt
    23101 tcctaactcg acgccgactg ctgctgcaat tttctctgct aatgggcgat tggaatttaa
    23161 cgcaaaaatt tttaatcttg ggtcaaagta atgttttgac atggggacct ccactttctt
    23221 tttcacaact ctagaattct acctaataaa tagtagtcat attttttaaa taaatcaagt
    23281 gattttgcaa aagtcttagt ttagataagg gagttttttt gcatatcctt ctttattgac
    23341 ttgtcttgct ctcgcaattg ccagcgcata ttctggaata ttgtctgtaa ttgttgaccc
    23401 ggcagcaatc gaactgtttt tagctacctc gacaggtccg atgatattcg tgctagaacc
    23461 aataaagctg tggtcgccga cagtcgtatg atgtttgttc tttccatcat agttgacgaa
    23521 gacaacacca catccgacat tgatttcttc tcccagcgta gcatcaccaa cataagtcaa
    23581 gtgaccgact ttagttcctt tgccgatttg agcatttttc acttccacaa agtttccgat
    23641 atggacgttt tcgccaattt cagcttttgg tcgtaaatgt gcataaggtc ccacatctgc
    23701 atgtttcttg acatggctgc tttcaatgac tgagttttca atgacgacgt gatcctcaat
    23761 cctactgtct acgatttttg aatgtgaccc gatgacacaa tcagaaccaa tgactgtatt
    23821 tccttggatt tgtacacctg cttcgatcaa tgtgtctgga ccgatttcta cgcctgcatc
    23881 gatgtatgtc gttgctgaat cgacaaagct tacgccattg accatatgca tctggttaat
    23941 tcgctttctc atgatctcat ttgctttagc taacgcgatc cgatcattga ctcccattga
    24001 ttcttcaaaa tcatccgttt gataagcagc aactgtatgg ccttcttctt ttaagatttc
    24061 gatgatatct gttagatagt attcaccttg agcattgttt gttccaactt tgcttaacgc
    24121 atcgaacaaa gcttcgttgt caaaacagta agttcctgta ttgatttctt gtacaagagc
    24181 ttcttctggt gttgcatctt tttgttccac gattttttct acgatcccaa tatggtcacg
    24241 gatgattcgt ccatatcctg ttgggttttc tgcttgtgcc gtcaagatcg ttgcacttgc
    24301 attttttcct tggtgatatt caaaaagatt gttcagcgtt tcagtagtca ataatggtgt
    24361 atcgccactg ataactaacg ttgttccttt ttttccttcc aagaaagaag cagcttgcaa
    24421 aacagcatgt cctgttccca attgttctgc ttgtaacgca tatttactgc gttctcctag
    24481 ttgtgcttta acttgttctg ctccgtgtcc aacgatcgta atcacttcat ctggtttcgt
    24541 ttcactgacc cgattaatga tatgttctac cataggttta ccagaaacgg gatgtaaaac
    24601 tttatataat tttgacttca tgcgggtacc tttccctgcc gccaaaataa ttgcataacg
    24661 tgcgtccaac tgcgtcactc ctaaatatag tttgctgatt tcagtttatc ccagtatacc
    24721 cattttttca acaaaacaag tgattttctt ttatcccagc gttttctgga taaaaaaata
    24781 gatagaccat aacgggccta tctatttggt aaccggtgct ctcctgccaa ctcctcggtc
    24841 acaagtcaca ctactcataa aacatgaaaa gctgtttttt gaaatcgttt cttgtgactt
    24901 agagttaaac ggtaggctta caacctcttc attaaacgtt tttcaatcag ctgaccttcg
    24961 gtattagctc aataaacggc aaaatatgag gagcaatttt tccgtttatt gagctaatac
    25021 tcaggacagg acgctgattt cacaacctct agataaacgg tgttcaaaag ccagtttctc
    25081 ggtattaagc caaatttatg aaaaaatgtg gaaagccatt ttcacaaaat ttttcttact
    25141 actcgaaact aagcggcttt ttcacaacct cttattctga aaaataattt cctggcacaa
    25201 ccgtaatatt tttcgtttga gtatctacat ctttgacata aagcagtgac gtataatcat
    25261 cgatggcgcg atgaccgttg aattttgatt cggcaaatac agtgatgcca acaagttctg
    25321 attcgaattc ttcaatcaag ctcttcattc cgttgactgt tccgccgcct ttcatgaaat
    25381 catccactac aaggacacgt gaaccacgtt tcaaacttct cttagacagt tccatctttt
    25441 caattctttc agaagatcca gacacatagt tgacacttac tgttgatcct tctgttattt
    25501 ttgagtcccg gcgtacaata acgaatggcg cattcagata ataggacaca gcttgggcaa
    25561 taggtacgcc ttttgtggct acagtcatta cagcatcgat tttttcacca aggtatttag
    25621 aagcaatgat tcttccaact tgacgcagca gttcaggttc tcctaacaaa tcagagagat
    25681 aaacatatcc tcctggaagc agacggtctt gttctgatag acgttctgct agagattcaa
    25741 tatatttttt cgcctcttca tatgaaattt cagggatgaa caatacacca cctgcagcac
    25801 ccgggattgt ttccaaggtt ccataccctc gttctttaaa tgttttcttg atgattccca
    25861 aatcttcact gatagaagat ttagcagatt cataacgttc ggcaaagctt gttaaggaaa
    25921 tcaattcatg cgggtgattc aataaatact gtgtcatgtc aactagacgt tcactgcgac
    25981 gtactttcac ctattccaac tccttttaag aaattttatc gttttacatt atataagttt
    26041 ttcttcaaaa attcgagcat tttatgaaaa aataagaaaa tagttcggta tttattaaaa
    26101 taaaaagaag cctataaaaa atttttcgtt tttataaggc ttcttttctt tataatttag
    26161 ttattttttc ttctgaaggc aaccaatatt cgtttgatga tatttacaat caggaacaaa
    26221 gcaataaaga ctaatgtgat tgttgctcct ggaggtgtat ctagataaaa cgagctagtt
    26281 agtcctgaca gcatccctag aaatcctaca caaacactga tagcaatcac gacattgaag
    26341 cttttgccta atcgcatacc gattgctgcg ggaagaacca tgattgcgga tatcaaaagt
    26401 gcaccggcaa tcgggatcat cacagcaata gccacaccag taatcacatt gaacatcatc
    26461 gacatcaaat gaacgggtag cccatccacg tgggcggtat cttcatcaaa agtcagaaca
    26521 tacatcggcc gtttgaataa aaagaacaat ataccgatgg caacgaataa gattcctaaa
    26581 aagatgactt gctcccatgt aatggtcacg atcgaaccaa acagatagga ttggatactt
    26641 gttgctgaat tccctccgct caaattcatc aatactaacg ctagcgccaa accaccagcc
    26701 attaaaatgg caatggagat ttctgaatag gttcggtaga tcgttcgcag atactccaat
    26761 atcacagctg ctaatacaac gactagcatg gtcgtaagtg taggattaag gttcaaaaag
    26821 aatcctaacg ctacccctgc taaagagaca tgagaaagcg tatcagccat cagcgattgt
    26881 cttctgatca ctaaaaacac acctagcatc ggggcgattc cggcaatgaa gatagcggca
    26941 agaaacgctc ttctcatgaa ttcatatgaa agcaacgcca ttgagaatcc tcctttctca
    27001 ccagtcggat ctgtctgtcc gcatactgtt tgatatcttc atgatcatgg gtgatcatta
    27061 gaactgattt cccatggaca tgcgcactat gtcggagaag acgataaaat tcatttctgg
    27121 attcttcatc catacctgtt gttggttcat caagaacaaa taagtcaggg tcagtagcaa
    27181 aaatacgcgc taaactgatt cgctgctttt gtccgccaga caattcacct attcgccgat
    27241 ttcgcatatc ccacatccct acagcttcta aagcacgctg tacatgttga tgatcttttt
    27301 cagagagacg cttgaaccag cggcctcttg gatatcttcc tgaacggact aattcgatca
    27361 ccgtactagg aaaacctgca ttgaaagatg caacttgttg aggaatataa ccgatactta
    27421 atttttttcc ttcttgattt ttctcagcaa tctttatcat tccttttgaa ggtttcagta
    27481 atcctaacga tgctttcacc aaggttgact tggctgctcc attttctcca gttaaaatga
    27541 caaattcacc cgcatctaca tgataagaga catcttctag tacaggttca tcatcatagt
    27601 aaaaggtcaa atcagatatt tcgatataac gcaccttaca acctccattc taaaacttaa
    27661 ttgatacttt cttttaaagc tttcagattt ttttccatga cagaaatata attttcgcct
    27721 gctttgattt gttcattagt caagctttct aaaggattca acacttctag cttcactcct
    27781 gtttcattcg acaatgtctc agctaccttc gaagaggcat tttcttcaaa ataaatcact
    27841 ttgatgttgt ttttcttcac aaattctttt agctcagcta agcgacttgg tgtgggttcc
    27901 tgatctggtg aaacgcccga aatcgctact tgttttaagt cgtattgatt cgctaaataa
    27961 gcaaaagcag catgctgagt gacaaatgtg cggtttgttg catcttttag ctcatcggag
    28021 tatttttgat ccagtgcttc aagctttttg atataagcat cggcattctt cgtaaaagtt
    28081 tcctttttat ctggatattt ttcgcctaat tcttcagcaa tcgttctgac ttcttttata
    28141 gctaataccg gatcgagcca aacatgtgga tcgtattcat gtgaatgacc atgcccttct
    28201 tctgattctt catgttcgtc tgcttcttgc gattctaaac ggtcgattcc tttagctgct
    28261 tcgatcatca aggtctgttt gctatccact gaatccttta acgaatccac aaaaaattcc
    28321 atgtctgaac tattatacac gaaaacatct gcatcggaaa tttctgctct ttcttttgca
    28381 cttggttcga aatcatgcgg ctccgttcca gcagggatca aaagtttcac gtttccttca
    28441 tcccctacga tctcttttgt aaaatcatac atcggataaa aagtcgtaac gatcgtcaaa
    28501 tcctcttttt tgtctgcttc cttgttcgta tttccacatg ctgcaaacag taaagcacct
    28561 atcattaccg tagctgtcaa aagatatttt ttcatttata tgctcctttc aatgaacaaa
    28621 tcggaatttt acgatttatt aacgtgaact aatgtatcaa aataaaaaaa cacagtcaat
    28681 agaaatccat caatttctga tttaagcatg tatattcttg attaaatcgt aaaatggatg
    28741 agttgctgct catatctagc catttcacta caataaaaaa ctgaagaata ccattttagg
    28801 attcctcagt ttttcatcac ctattttact tcttatattt tattgcaggg ttcgtactaa
    28861 ataaacttcc tcacaaaaac cttttaaccc attatatact ctctgcgctc ttgaataatg
    28921 ttgacacaag gcaaaaacgg ttggtccgct tccactcatc aatgcagcat ctgctccgta
    28981 cttgatcata cgctctttga tttgatggat gactggatgc cgctttcccg ttatatcttc
    29041 taaactgttt cccatttttt tgatcatctg ctgataatct tgctctcgga tggcttcagc
    29101 aagtccgtgg atgtcttgat gttggatggc ttgcgtatct acttctctaa acactgtacg
    29161 tgtagagaca ctcattcttg gtttgaccaa cacaacccag cactgtggca tagaaggtaa
    29221 tacttggatt tcttcccctt tgccaccgac aaatgccgta cttccgtaga cacagtaagg
    29281 aacatctgta ccgacttcca aaccgatttc tgccagttct tctaacgaac agcccagatt
    29341 ccataattgg ttgatccctc ttagagcagc tgcacagtct gtacttcctc cgcctaatcc
    29401 agctgctact ggaatattct tcttaacaaa gatcttgaca ccggttttga tattgtattt
    29461 attttttagc aataaagcag cttgataaac attgttccgt tcatctaccg gtaaaaatgc
    29521 tttatttgtt tctacaatga ttttgtcttc tgttatttct tcaaaaaata gatgatctgc
    29581 caagtctacg ctcgacatca ccatttccaa ctcatgatac ccatcttgcc gtctatataa
    29641 tacatccagt cctaaattta tcttggctgg cgctttttca atcaattcca tcctctcacc
    29701 cattcgaaaa gtctaagtcg caagtattct atcataggga aaaataaaag tatagcttat
    29761 gtctgcctag tggttgattt tgtcatcgtt tcaacagctt tcacagaatt ttctcttcat
    29821 aatccaaaca agttctagtg attcttataa ttctgtgagc acttcttctg ccaaactcgg
    29881 aacatttcct ttttcttttc ctatcagact gagtagctgc atggtagcaa atccgaaatt
    29941 acttcctttc ctataagcaa aataacgtcc ttcccaatga tcgacatatt ttaatttctt
    30001 ttcataagca gactggaagg aatagaaaaa attaccataa ttatatacga tatgcatgac
    30061 tttttcttga aagaaagaaa aatctgtttc tccaacatta gctaatggag ctaagcctag
    30121 attggcttcc gagtagccca ttttctcgta tacaataaac agattggcta acagatagtc
    30181 agctaattcc tcagattctt taggtaagac ccgcaacaaa tcatatcccg cctgcttctt
    30241 cgtcatcggc tgagcggtga tgaagccaac gactttttga tctttacgga caataccgac
    30301 accgctagat aatacatatg atttatcaaa tcgtcccaca gagaagtatt tttctcgtgc
    30361 accttgaagc catttttgtg aaacttcatt tacagcttga tgtaattctt ccggcagttg
    30421 atcgtaccaa gtaaaatggt atccttcttt catcaaacgt tgatattcaa gttgctgact
    30481 cagccgagac aagtcttctg ttgctagatc aacaatccct gcttcaccta ctttcgtgaa
    30541 gtgaaaaccc aattcatgaa gcatcactac gtagctttct gatatcttat aaaacgcaag
    30601 ctgatagcct aaacaatctg cttgtttcat aaactgattc gttgctgccc gcatcatttt
    30661 aggattgcca actggatctc ctaaaacaaa gcatttgttt cctttgatct gataaccaaa
    30721 aacgacttga tcttggtcgt cttcttggta ataaaaataa ctgtaacctg acaagcgaag
    30781 ctgatggctt gtagctgtgc ctccgtatgt ttccaataaa cggtcaaaac ggtctttttc
    30841 gtactcactg ccaagcttcg tagtatcttc agctaaatat tgataaagcg ccactaaagc
    30901 tagcaatgag atgcccaaac caataagccc tgagaaccac acatcatctg aaggaaacaa
    30961 aataaaatga tttcctgtta ttcgaccgac taaaaagcgt ccgctgtgat agcctgcaat
    31021 tgcgtaaacg ataaacaaaa agccgaacaa gctagcatca aagattatag ctccccaaga
    31081 ataaacgaat tttgttcgat aaaattcttt tctggataac cacacagcta acagaatcaa
    31141 gacatatacc aatatcaact gccaagataa cgtacgtgtg attgtattta aaataccaaa
    31201 agatagtaaa gcaatcgtag gcagaaatgc ctttttgact tttactgaaa gtgctctagc
    31261 aaggccgagt aataaaaagc caaccaatag attcactgtc tgatctaaaa aattgaatga
    31321 gaacggcaat aatacttgaa atagccgact gattgcagac agattagtga ttgttgatag
    31381 aagtaccatc atgatccccg caaaatagac agcacttacg agcacgatat gcgcaacctt
    31441 ttggaaaaac agtcgcggaa tgttatctaa aaatcgattg attttgactc ctgtatgtgt
    31501 gatgaacaat agaagtcccg tcataaaagg tagaacataa taaaacaacc gataatataa
    31561 tagccaaact actgctacat cttgccgaac agacaattga ctcattccta agatcatcaa
    31621 aacgtcaaaa gtccccattc cacctgggac catcgtcagc attcctatca atgtagcaat
    31681 catgaacata ggatagatcg aaagcatcga aatatctatt ctcatcaact tgccgatgat
    31741 caaaaatacc gcaagggctc ccgtccattg tccaaaggat gccagtagca gaccaagcaa
    31801 gccttttgga taaaactcga agaacaatgt ctttctcttt agataagcaa aaaagaaaat
    31861 gactggtgct aaaaaactac cgccgaaaag ccagatccaa tagcttcgaa aaatactttc
    31921 tgactgaata aaaaagacat caataaatgt aagaaacgac catacagaca aaccagtcag
    31981 cataaatagt gcgacttttg atacagtggc taaaaccatt tttctatttg tctgctttcc
    32041 ataaaaacta gcccgcagac ttgctcctac cacaccacca aaaccggcga gattgttgat
    32101 cgtgtttgtt acccacgcag caagaagaaa ctcttttctt tccatttttg gttttccttg
    32161 tttttctaag actctgatcg tcacccaatc atacaacaac atcggagtga cacccagcag
    32221 tccagccaga atcatgagca ggatcgtcat gcggctttgt tgtcccatcc tttgccaaat
    32281 atcttgccaa ctcatgccat tagcaatatt tgcgacttga tttgccacaa agactaacac
    32341 ggagccgaaa aaaatcagtt ttaaaatcag actatgttcc tttatccatt gcagcatcaa
    32401 gtatcgttta tttttcacat tttcccattc ctaacttgta tacttttgtg tcatacccca
    32461 atgttatctt atttatttta atttttccac tttagacctg ttctttgctt gcgatgatag
    32521 aaaaagaaag atatcctaat gctaaaggag aggataacat gaaagtagga tttatcggca
    32581 cagggaatat ggcacaagct attattgatg gtttattaaa aaaaggaaca gtctcgaaag
    32641 aagaggtgtt cgtttccagt ggacattttg aaaatgcaca aaaatttgct gctgaaactg
    32701 gcgtccatgc atgcaaaagt aatcaggaag ttgcatcaca atcagatatc gttcttttag
    32761 ccgtgaaacc aaaaatcatt gaaaaagtat tggaagaact aagagaatat agccagaaag
    32821 tcttattcat ttctattgct tcaggaaaat cattaataga tttagaaaca gcgcttgaag
    32881 atcctcaggc ctccatcgta cgtgtgatgc cgaatgtcaa tgtcagcgta ggagcaggcg
    32941 tttctgcgat ttgcaaaaat gaagctgcca cggaggaaca gcttcagcaa gctacgatga
    33001 tttttgaagc aatcggcagc gtctatcctt tagcagaatc agatttcagt acattcattg
    33061 gccttgctgg cagctcaccc gcattcgttt acatgctgat cgatgcgatg ggacggactg
    33121 gtgtactgaa tggtttgtcc aaacagacag ccactgagat tgctgcaaaa gcaattatgg
    33181 gttcttgtca aaaattcctt tcaagtgata aaaatccatg ggaacttgta gaccaagttt
    33241 cttctcctgg cggtacaact gttgcaggtg tagttgcctt agaagaagca ggattcatcc
    33301 cagcaatcat caaaggaatc aatacgacga tcgagaaaga tcttgagatg ttgaataaaa
    33361 aataatgata gcaatgtttt ttcgtgcctt acaaaaaaag aagagctgtg gcttttgccg
    33421 cggctcttct tttgtatttt ttattattta gcataatcag ttgcacgagt ttcccgaata
    33481 acgattactt tgatgtgtcc aggataatcc agttcttctt cgattcgttt acgaatatca
    33541 cgtactaaac gaacagcgtc taaatccgag atttcttctg gcttgaccat tacccgaact
    33601 tcgcgtccag cttgaactgc gtaacttgat tctacaccgg cgaagctgtt cgaaatattt
    33661 tccagacttc ttaaacgacg gatgtagttt tctaatgatt cacttcgcgc accaggtctt
    33721 gctgctgaga gcgcatctgc tgcagctaca aggacagaga taacagaagt agcttccaca
    33781 tctccatgat gagaagcgat cgcgttgatt actactgggt tttctttgta cttggcagct
    33841 aattccgcac cgatctcaac gtgagatcct tcaatctcat ggtcaagtgc tttcccaata
    33901 tcatggagca acccggcacg ttttgctaat tggatatctt cgccaagctc tgctgctaag
    33961 ataccagcta attttgcaac ctcgaccgaa tgcttcaaga cgttttgtcc ataactagtt
    34021 ctgaatcgca gacgtccgat gatcttgatt agatctggat gaagtgtatg agcaccgact
    34081 tcaaacgctg cctcttcccc gtattcgcga atacgttcat ccatttcttt acgagatttc
    34141 tcgaccattt cttcgatacg cgctggatgg attcgtccat cttgtatcag tttttccaag
    34201 gtcatccgtg cgatttctct tcggattgga tcgaatccgg aaagaacaac tgcttccggt
    34261 gtatcatcga tgattaaatc gataccagtc agcgtctcaa gcgttcgaat attacgacct
    34321 tctcgtccaa tgatacgacc tttcatttca tcattcggaa gggtaacgac tgaaacagtc
    34381 gtttctgaaa cttgatctgc tgcgcagcgt tgaatcgcta atgataataa gtttttcgct
    34441 ttgcgatctg cttcttcttt ggcacgctgt tcagattctt ttaccattag agttaactca
    34501 tgattcagct cttcttcagt cgatttcatg atgacatctc gtgcgtcctc ttttgataaa
    34561 ccagcaattt tttctagttc tttttgttga tcttcaatca ttttttcaac gtctttttct
    34621 cgctcttcaa ttaattgctg ttttgaacta agtgcttctt ctttactttc aagtgagttc
    34681 tcgcgttttt ctaacgagtc atctttacga ttcagcgttt gttcgcgttg aatcaaacga
    34741 ttctcttgag attttaattc taacttactt tctctcagct cactttcaac ttcagaacgg
    34801 tatttctgat tttcttcctt agcttctaac aatgcttctt tttttagagt ttctgcttct
    34861 ttttttgcac tatcgatgat tccagcagct gatgattgtg caccagctat ttctttctcg
    34921 tgacgtgact ttgcgaccat aaaccctaaa ccaagaccga caattaaacc gatgatagcg
    34981 aggagaatgt tgaataccat aaaatccacc tccatactat ctttttcatt tttatcatct
    35041 atacagcttc tatgataaat tattctaaac tcgtcaatat gcatcttcgc atatatgtgt
    35101 acaaaaaaca tgcacaactt tattctaatg tttgtacccc gtagtgtcaa ctttaaataa
    35161 acaaagaaac ggctttctac ttattcatta aacgatggtt tttaaaataa atgacactag
    35221 tttcatatag gtaaaccttt aaattgagaa acttttctat taaattattc tttgtagatg
    35281 aacagatatt tttattttat gtacccactc gtataattaa ataactcacg atttctttat
    35341 tcaataaaaa aacggcaggt caaggagagt acctactctt cagaccagcc gtttttgttt
    35401 atcataattt ttattcttct actaaaggca attcttcttg tcctttttca tcttctggga
    35461 cagcgacttc ttcaccaata ccataagctg ctctaactag agcagaaact tctgccatca
    35521 tttcaggatg attagccata tagattttag cgttttcccg tccttgacca atccgatctt
    35581 ctttataaga ataccaagca ccgcttttat caacaatgtc tttttctact gccatgtcta
    35641 gaagttctcc ttcttgtgag atccctaaac catacatgac atctacttca gcgattttga
    35701 aaggaggtgc tactttgttt tttacgactt tgatctttgt acggttacca acaatatctg
    35761 taccttgttt caattgttct gcacgacgta cttccaaacg gatcgttgcg tagaatttca
    35821 atgcgcgtcc tccaggagtg atttctggat tcccaaacat cacgcctact ttttcacgga
    35881 tttggttgat gaaaatagcg attgtttttg ttttgttgat cgaaccagag agtttacgca
    35941 atgcttgaga catcaaacgt gcttgtaacc cgacatgtga gtcacccatt tcgccgtcga
    36001 tttctgctcg tggaactaaa gcagcaactg aatcgactac tacgatatct acagccccac
    36061 ttgatactaa agcatcagcg atctctagac cttgttctcc tgtgtcaggc tgtgaaagaa
    36121 gtagttcatc gatattcaca cctaattttt gtgcatattg cggatctaac gcatgctcag
    36181 catcaatgaa agcggccgtt ccgccatttt tttgtacttc tgcaatagcg tgtagtgcaa
    36241 ctgttgtttt acctgaactt tcaggtccat atacttcgat gatacgtcca cgggggtaac
    36301 cgccgacacc taaagcgaca tctaacgcaa gagagccact tggaattgta gagatttgtt
    36361 ggtcgatttt ttcgcccaat ttcataatag agcctttacc atagctcttt tcaatttttt
    36421 tcagtgcagc atctaaggct gctttacgat catctgccaa tacgataatc ctccttatat
    36481 ttacaaatca gagcaacatt tcattatcct tatcatatct tttttagtga aaaaaagcaa
    36541 gaaaaaaccg aacaaatatt cgcatttatc attttcttaa aagctgtttt ctcaaaagat
    36601 caaatccttg cataaccgca ttatatctga tagcagcacg atctctggca aagtgatatt
    36661 tttccgaaac cacgccgtct ttagatgaaa tagcgatcca tactgttcct gcagattgac
    36721 cttctaacgt gtccggtcca gccactccgg tgaaagctac actgtaatct gttcttgcta
    36781 tctcacgtgc ttgaatggcc atctgctcca cacattcttt gctgactgtt ccatattctg
    36841 ctaatagctc cggatcaatc tccagaaaac cagcttttgt ttggagcgag taggtaacga
    36901 atccgcctgg aaatacttca gatacaccag caatgtcgcc taatgcggct tggaaggcgc
    36961 cggctgtcaa actttccgct gctgttacag tctgcttgtt ttctttcagt agttccacta
    37021 cgacttttgc tagcgaatta tcatcgccgt atccataaaa atattcccct actcgttctt
    37081 ggattttttc ttctaatgct aaaagagctt gattccctgc ctgaacatca tttgttttca
    37141 ctgtcagtcg taatgtcact tcatttggtt tggcatatgg tgcgatcgtt ggattgatct
    37201 gtgtctcaat cagatctttt agctcagtga ccagtcgcga ttcaccaatc ccataaaagc
    37261 gcaaaacttt agaaattagt ttttcttcag aaggaaaacg ttcctctagt aaaggacgta
    37321 cttgttccac aaacattggt ttcaactcat taggcggacc gggaagaagg atatacgcat
    37381 gtgtatctgt ttgataaaaa gtccctaaag caagccctgt acgatttggt agaggaaccc
    37441 cgccttcgat aatctgggac tgttgcagat tgtttttggt catttttcga tgggttgttt
    37501 caaaatatgc caataatttt ttgtatcctt ccgtattttg tatgagagac ttaccgaggt
    37561 gagccgcagt tgcttctttt gtcaggtcat cttctgtcgg tcctaatccg ccacataaga
    37621 gtatgagttc acttcttgtt tcagctagag caatcaactc ttctaaacgc tgctgattat
    37681 ctcctacaac tgtttgataa taaacttcga tacctagatc agctagctgt tctgacaaga
    37741 aagtggcatt cgtattcacg acttgtccta gtaaaagttc tgttccgaca gctattattt
    37801 ctgctttcat tacttcccgc tcccttatat agtatacgct gtatttttat atttttttat
    37861 ttaaaaagag ggcaaggagc tgtgacaaaa atcttgtcac agctccttgt tccaaataaa
    37921 cagtgttctc ctgccaaaac ctcggtcata agtcacaata tacataaaac atgaaaagct
    37981 gttttttgaa attatccctt gtgactcaga gttagacggc aggctcacaa cctctccact
    38041 aaacgatact tacattgatc ctttgaacac accgctgttt ttgatgaagt aatccacacc
    38101 tgaatagata gtaaagatca gacaagcata aagcatgatt tgatctactg gaataccgat
    38161 caggctgaat gggatattat tgataaacag caagataata gcaaccattt gtgtagccgt
    38221 cttgactttt cccggccaag ctgcagccat gacttctcca tgttcgacaa gtagtaaacg
    38281 caagcctgtg acggctaatt cacgacagac aatgatcgct actacccaac caggggcctt
    38341 tccttggtca accaacatga taaaagcagt cataactagc attttatctg ccaacgggtc
    38401 cgcaaacttg ccaaaattag tgacaaggcc tcgcgcacgg gcaatttttc catctaacca
    38461 gtctgtgata ctagctaaag caaaaataac tgcacctact agctgtgtga cagccagtgt
    38521 ggtgtctcct acgttaaatt gtccccaatc taaaggtaaa cctacaacca aaatgaaaat
    38581 cgggatcata caaattctaa gtacagttaa tttatttggt aaattcacac tatcgactcc
    38641 tttcttccct attctacgtg actatcagcg aagtttccac cgatattatt tacttttcca
    38701 aatgaattta ttcaaaggag tatttttatt gaaattgatc tttttgaatt aacgaagaag
    38761 gaccgcgaca cagatttgtc acgatccttc ttttttatac ctacaagtaa acagatgcgt
    38821 aaactaattt atacgatcat tctgcgtatt gaagagtaag gttcacattt tttggactct
    38881 gattgttttc ccctggatta actggtacgg attctccatt cgctttgatc gttgcattgc
    38941 ttgctgcacc aatcgtgatg gtcgcatttg ttgctgtttc cggtaacacg gtactctgtg
    39001 tttcatttgc tgccagtgtc ccttgataaa cataggcatt gtctactagc acacctaacc
    39061 atacacgatt gacagcagta aattcaaggg ttacaggctt ggtagcgtct gtgatattta
    39121 ctgcgatggc actgccagaa tcttctcctg tagctattgc cattttcttt tcttcacttg
    39181 attttgttgt tgattctact gtagaagact ctttggtctg cgtgctttgc tctgaggaag
    39241 aggactcctc gactgatcct tctactttaa gcgaagaagc tgtgtctccg attattggat
    39301 cagcttgacg atcttgccaa gtcatatagc ccacgacaac gacaatcgcc aaagcaacca
    39361 atcctaataa aatgacagga agacttgtcc aaaagcgact tggatgcgat tcttcctgat
    39421 gcattgcttt tctagaagta cttactgttt caggctgcgg acgttcaggg accgatcctg
    39481 tcaatttgct tttcccatca aaaacatcga ctaaatggtc tccatcttct cctacagctt
    39541 cagcgtattg ccgaataaat gcccgcacgt aaaatttacc aggcaattgg tcaaaatcat
    39601 cattgtcgat tgcttccaaa taacgttttt gaatttttgt aatctgctgt aattcatcta
    39661 aagatatttt tttattcagg cgtgcttgcc gtaatttttc tccgatggtt tcaaaagcca
    39721 ctcgttcatc ttctccagtc tgtttgattc tccgaagcat ctgctgttgc aacgaaagac
    39781 gttatgtttg ggtgctgtga caaaagtcat gtcacagctc cttttttccg aataaacatt
    39841 gttctcctgc caactcctcg gtcacaagtc acaatatcca ataaatataa gaagctgttt
    39901 tttgaacttg ttccttgtga ctcggagtta aacggcaggc tcacgacctc tttcttaaac
    39961 ggtgttcaat cagctgttcc tcggtattag ctcaaaaacc ggcaaaatat aaggagatat
    40021 ttttccgtat gtttctctaa tactcaggtc aaaacgctga tttcacaacc tcttccttaa
    40081 acggtgttca aaagcttgca tctgcggtaa taagtccaac caagttaaaa acattaagaa
    40141 gctgttttta cttggctgtt cttattactt gatgcagggc agctttttca caacctcttg
    40201 gtacacggtg ttccagaagt agcttcttcg gcaataagcc gaaatatcac aaaaatttga
    40261 gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
    40321 tttgattact tcgtttgatt ttattttaat ataagttgat taaaagagag catgatttgt
    40381 tttattttct cactaatttt tccaacacaa ctaagaatac cataaaagct gattttggag
    40441 gaggttccta ctacaagtct aaaacgaaat ttaagacttt tcctcataaa atactggttt
    40501 attttggtct catgtaaaac tcgctgaagg cctcttcact tataaatgct tccccagctg
    40561 ccagtacgtc tttcatttgg atcgaatcga tgatttcagg aagatcaaat aaagttcgat
    40621 cgccaaataa actttgcgta aattgattgg caatatattc gatcgaattt aatgattgga
    40681 aatattgtcc aagcattttc ttcttcagca aatcgagatt tgtttctgac acttccggat
    40741 catcagcgaa tccaagaatg atttctttga ctttttctgc tgctttctct ggttcgtccg
    40801 tatctccgct gaagtccgca aagtgaaatt cacgatccaa gctgaattca aaaccaaagc
    40861 tgtcatcgat aattccttga ttgtacatcg ctagatagtt tcgagaagta ttgcctaaaa
    40921 tcatttggaa caacagattg attgctgttt tatagcgtaa cagttctgtt ccttcttgcg
    40981 gcaatgtgtc taatccttta ataccaagta caaatttatc tcgtgtaatg gctgcttcca
    41041 atgcactttg tttgatgatt tcttttgtgt tttcagggaa ataacgtaca atttcttgct
    41101 ttggagggaa attttttgct tcttggtttt cccgaataag tttcatcaat ttctcaggct
    41161 ccatcttacc aactacaaat aaaaccatat tgcttggctg ataaaacgtc cgatagcacg
    41221 tatacaagtc ttgagctgtg attttgtcga tactttcaac cgtgcccgca atatcgatat
    41281 gcaatgggtg tgtaggatac aagttattta gtatgccaaa aaacatccgc cagttagggt
    41341 cgtcttcata catttggatt tcctgaccga tgattccttt ttccttgttt acagtttctt
    41401 ctgtgaagta aggtgcttgt acaaaatcga tcagagtcgt cagattcttt tctacctgat
    41461 ccgtcgtaga aaacagatag ctggttttcg taaaacttgt gaaagcattg gcggaagctc
    41521 cttgtttccc aaataattga aagacatcac catcttcttt ttcaaacaac ttatgttcta
    41581 aaaaatgggc aattccatct ggaactttca ccttttcttt ttcaccatag ggaataaatt
    41641 cgttatcgat cgaaccataa ttcgtactga acaaaccata tgtcttgtga tagtcatttt
    41701 taggcaacaa atacacggtc aaaccgtttg gcagtacttc atgataaagt gtttcattga
    41761 tttgctcata ttctgtttta ttcatttgca tttcctcctg ctaaaaagaa gattgcctgc
    41821 aattcgattt gctcagctac ttgttgtatc tctttgatcg ttacgctctc catacgtttc
    41881 aaccattctt catcagataa ttttgtttcc ggcagccagc tgtccaaaaa agaagcttcg
    41941 attgccgctt gcggagagtc tagagaaagc agatattggt ttctcagcat tgcttttgtc
    42001 tgagacagtt ccaactctgt tatttcacca tttcgcaaac tttctaattg ctcgtgaatc
    42061 agatgaagta cttgatttcg gttcttttca tcgataccgg tttggacact catgaatcct
    42121 cggaacgtat ccacgctact agaggcataa taagctagac tttctttctc tctgacattc
    42181 atgaaaagtt tagagtgagg aaaaccgcca aataaaccgt taaagaccat tagcgcaaaa
    42241 cgttctggtt catcgtaata cacatttgtc tgataagcta ggttcagctt tgcttgaacg
    42301 attggctcac gtacttgtcg ttccttcacg atattcactt gaggttgggt ataaaacaga
    42361 tctggataaa ctgtttcccg cgactcaaat ggtaattgcc caactgcttc agctgctttg
    42421 tccggatcaa catcgccgac aacaaaaatg tcgatttgat catttgccat catttcttga
    42481 tagtaggacg ctagcgaact agcagtgatc ttgtccaaat cttcgactac gcccaaccct
    42541 gggatctttt gttccgggct gttttcaaaa taaagttctt gcaaggcata ggaagcaaaa
    42601 gtttgtttat cttccttcaa actttccaaa tatagacgca gattgttctt ttccaagtca
    42661 aacgtcaact gatcaaactg atgattttta atatttggat aaaataaaac ctcttttaaa
    42721 aaatctaccg cttgagaaaa aaggtttgga tcattgacat attcaccatt cacaaaactg
    42781 atccctgcgt ttagccaatg taaatttcct ttttttctaa ctgataatcc aaagcttgcg
    42841 ccataaagct ctgcaagttg cgcacttaac tttgtctgat ccgaataatt caaactattt
    42901 gtttccaaca tgctgctgag aagtgaacgt ttcgtgatga cttcttgttg cagacgcgca
    42961 gtaaaacgga taaaaatccg gatcgttttg tattgttttg ttggtaagac atgaagattc
    43021 acgccttctg ccaattgata caccatattt atatgctcct tttcacagtt gattgcccgc
    43081 ttactctttt aatgggtcaa cctgcctttt ttacttattc ctctgcttga aatttataag
    43141 ttcaggcaat tcgccgtttt ttctaaatga tgttacactt tatagtatag catagatgtt
    43201 ttttatcaaa agaaacagac ggctaaaagc gtatattgtt tttttatttt ttatttttcg
    43261 atatactaaa gacaaataaa agaagggagg gtcattcatg atcaaagaat tcaaagagtt
    43321 catcatgcgt ggagatgtct tggatcttgc tgtcggcgtt gttattggta gtgcgtttac
    43381 aagcatcgta aaccaagtgg tgcaaggctt gatcacacca ctaataggtt ggtttgtcgc
    43441 aatgatcaca ggtacttctg atttagaagg agcactttca attcttgact ggtcgccgac
    43501 aaaaggcgtc acatttgcgt ttggtaacgt tattagtgct atcattacat ttctgatcac
    43561 tggttttgtc ttattcttag ttgtcaaagc ggcaaaccgt gcgaagaatt tacgctccaa
    43621 agaagagcaa gtggaagtgg aagaaactcc tactgcagaa gattatctta gtgacatacg
    43681 cgatcttctt gccaaacaag tggcagaaga caatacacat ccagtagagc aaacaaatct
    43741 ttccgatact tctaaataat aaagaatcta aaaaaaaaga aagccgattt tcagtgtcgg
    43801 ctttcttttt ttagattctt aacttgctac ttgttccttt ttcatgtcga tatgtggaat
    43861 atcatcttcc aaatacacat ctgagattgc cttgaatcca aacgactcat aaaaagcttg
    43921 taggtattct tgtgcgccga tcacgattgg tttttctgga aaatttttct ggatcatgtt
    43981 aatcgtttct tgaacaatct ttcttcctaa accatcttta cggttgtcct gatgaacaag
    44041 gacacgtcca aaatggatac tctccgcttc ttcatatact cgcgtgtagg caaggatctt
    44101 cccatcagta tcttgaaaaa acgtatgcca agcttctcca tcaatgtcat caacttcttg
    44161 ataaggacac tgttgttcca caacaaaaac tgcgattcgt tctttgacga tcgcgtaaaa
    44221 ctctgctgtt gacaattctt caaatttctt tacctgatat tccactaagc aaacgccccc
    44281 tgctttttta ttcttagcat accgattcgc acacaaaaaa gcaaacgaac cttacgaatc
    44341 tcaggctcgt ttgcttttta aactgcttca gttaaaaatt cgatctcaac tgtccgagta
    44401 agtacatctg aatagctgta agatactcgt tcaaaagaat tttcttcagg atccaaatca
    44461 acaacgaata ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt
    44521 cttcctgtct gagcaaccaa catgattggg ctgccgatac gaccttctaa atctttcttg
    44581 attgaagcta aagttgttgg catacagttc acctctacta tcaactatac cacatatttt
    44641 taaaattttc aagtttacca taaaaaaaaa aagaaatgca ataaaaataa ataagaagct
    44701 cattaagatt tttctaaatt attcctcatt ataaaatttc aagtgttttt ttattttgag
    44761 ttttactctc ccgtaagcgc tcattacttg cttgtgatcc attgctaaac attcagccat
    44821 gtctacaaaa tcaacgcctt gcatataata atgaaaaatt ttagcttcta tttttgaaaa
    44881 ctggatttcg caattttcga ttgcttcttt taataaaaga tgctgaattg ctaaaggttc
    44941 ttgcaatgtg atctctgcta aaaagtctgg tccttctgtc tctatttttt ggtcgattga
    45001 gacttcatct ttttgatcac gacgcttcag cgcgttctgc ttcctcaaca gagaatagat
    45061 ttgattgctt ataacgcttt tataataact tccaaagcta acacctttgc ttttttcgta
    45121 agttctcaat gcctgatata aagcgatcct tgcttcttgt tcccaatcct cagaaacata
    45181 atttcttaaa taataggtgt ttttcaaacg tagtaaaagt ggaaaatatt tttgatacag
    45241 ggcagcaaat gcttcttcgt caccattacg tgcagcgcgc aataactcct cagacataaa
    45301 ataattcctc cataaattcg tttatggagg aatcttatca tcaatgattc atcgaactga
    45361 ttagaacgat atgttattta ttttttctca ttaaatcgtc caatttttgg gacaattttt
    45421 ttaactgctc ttcattccat ggagaattac gtcggaaatc aataaaatgc atatctttat
    45481 tttcttgggc tattgttttt tccgtttttt ttatcgtctt atataattca taagcagaag
    45541 ttcgtaatgc tccttgggaa aagatcatcc actgttctgc taaatcgctt gtcgccactg
    45601 tcacttgtgt caaacggtca ttcagctgac cagcgattct ctcgatataa ctgtcagccg
    45661 tttcgtcttc tttcgtaaaa acaacatcca actggtattt cttataattt tgttggatgc
    45721 ccggaactag ttgggcatcg aagacaacga tgacttccat tccttcatat ttcgcataat
    45781 ttgataaacg atgtaaaaga gcttctcttg catccgccat tttgtcctgc tttttaagct
    45841 ggacaagttc cggccacgaa ccaatcatgt tgtagccgtc tacaatcaac agctgttttt
    45901 tcatggtcta ctccccctga cgttgggaaa aagcctgata cataagcaaa ccagcagcaa
    45961 cactggcatt taagctctgg acgtgcccgg tcatcgggat cgtcagcaat tcatccattt
    46021 cttttttcaa tcctggactc attcctttgc cttcattacc aatgactaaa gcaattgatc
    46081 ctttggcatt ccatttttgg aagtttgtgc cggtcatatc tgtgccaaat acccaaaatc
    46141 ctgtttcttt gagctgttgc acagcttgta ccaaattcgt cacgcgtgca acagggacat
    46201 gttcgactgc cccagtggaa gtctttgtta cgataggtgt gattccaact gctcggtgtt
    46261 ttggtatgat cacaccatcg acgccacttg cgtctgctgt ccgtaaaatc gacccaaaat
    46321 tgtgtggatc ttcgatgcta tccaaaatca aaaagaaggg gtcttctttt ttctcttttg
    46381 ttcgttcaat cagttggtca agggataaat attcataagg tgtaatcgca agaacgactc
    46441 cttgatgcac gccgtgatcg ctcaaagttt ctaatttttg tttaggaacc catttgactg
    46501 gcacagcttg ttcacgtgcg atttctttca gttcttctac tttttctcct cgtgaatctt
    46561 cttgaagaaa gagtttattc cctcgccctt gttgtagcgc ttctactgcg gcatggtgcc
    46621 caaagacgaa attatcttct aatgcttctt cattttgatc agaaactgac tgatttcgtc
    46681 cagtttgctt tttatttgaa tatctatttt gttttttgtc ttcaaatcca cgtttcgatt
    46741 gatgtttgtt tgttctttct ttacccattt ttttcaccaa ctttctgaat acaccagcgg
    46801 atcaattctt ccatccgctc ggtttgtttt gtcaaatgca gatagcccat gagcgcttcg
    46861 aaccctgttg cgacccgata cgtcgtaaca tcagcatttt ttgcgctcgt atggctttta
    46921 gcatttcttc ctctgcgata ataaagttct tcttcttctg tcaaaagatg ttcttccagc
    46981 atttgctgaa tcaaaaaggc ttgtgcattc gcagaaacat aattcgttgc cgcacgatgc
    47041 agcttattgg gttttgtttc tccttgatca atcaggtggt cacgaacata gatttcgtaa
    47101 atcgcatctc caacataagc caaggctaaa ccgttcaatt gtgtgtaatc tctcatgctt
    47161 ctcttctcca tcgggttcct tgcggtgtat cttctaataa gatccctttt tctttcaaca
    47221 aatcacgaat ttcgtcgctt cggacaaaat cacgattgct tcttgcttga tttcgttctt
    47281 cgatcaattg ttcaatctct tcatctaata actcttctgc tgtaaataaa ataccgaaga
    47341 tcgaaagcca ttgcgagaat aaagcttctc cttgtgccaa gacagcggca gaaactttcg
    47401 gctggtctag ataagtgttc agccacttag caagctcata gaccactgtg atcccatttg
    47461 ctgcattaaa atcatcgtcc atttcttcga tgaatcgctg ttctaaattt tctaacaaag
    47521 cgattttatc cgcatcatct gccaaattct ctgaggcagt ttcttggcgg aatttcaaat
    47581 tgtcaaacgt attcttcagt cgttgtaaat tggtggctgc ttctttcatc gtcgtttcac
    47641 tgtagcggat cggacgacgg tattgtgtgg tcgccatgaa gaaacgcaag acttgtggat
    47701 caacgttttt gatcatttca tgtacagtga tgaaattccc tagagattta ctcatttttt
    47761 catcgtcttc gccaatcgtt acatagccat tgtgcatcca gtaattagcg aattttttcc
    47821 ctgttttcgc ttcactttgt gcgatttcat tttcatgatg aggaaattct aaatcttgcc
    47881 cgccaccatg gatatcgatc gtttctccta aatgttttgt tgccataacg gaacattcaa
    47941 tatgccaacc gggacgtcct ttgccccatg gtgaatccca agaaatctcg ccaggttttg
    48001 cttgtttcca taaagcgaaa tctaatgggt cttctttttg ctgctgttct tcccctgtac
    48061 gctgacttgc tccgacttcc agttcatcga tggattggtc acttaatttc ccataatttt
    48121 caaatttacg ggtacggtaa tacacatcac ctgaagattc ataagcatag cctttttcaa
    48181 ccaacacttg aataaatgct aaaatatctg gcatatgatc catgactctt ggatgcagtg
    48241 tagcaggttc gacattcaac gctttcgtat cttcttcaaa tgctcggatg aaacgatcag
    48301 caacttctgg tgctgtaata cctaattcgt ttgctgtacg gatgatttta tcgtctacat
    48361 ccgtgaaatt cgatacgaaa tcgactttat agcctcgata ttcaaaatag cgtcgaattg
    48421 tatcgaaagc aatggtgcta cgagcatttc cgatatggat ataattatac acagtcggtc
    48481 cgcagacata cataccgaca tgtccctttt gtatagggat gaattcttct ttttttctgg
    48541 tcaatgtatt gtagattttt atcattctga tctcctcact ttttctcctt tgattcgaac
    48601 gacttttgct ggtactccta ccgctgtagc gtcgtctgga acatctttca acacaacggc
    48661 acctgcaccg attttggcgt tttttccgat cgttaccggt cctaatatct gcgcattcgc
    48721 agagagcatg gctccttgtt taacagttgg gtgtctttta cccgtttctt ttcctgtgcc
    48781 gcctaatgtt acgccatgga ataaaatcac atcgtcctcg atttctgcgg tttcgccaat
    48841 gacgattccc attccatggt cgatgaacac gccagttcca atcgttgctc ctggatggat
    48901 ctcaacacca gttaggaaac gccagaattg agcattgatc ttcgccaata aaaagagccg
    48961 gtgctgatat aaaaaatgag agaaacgatg ccaaaacaag gcatgaaaac cagggtatgt
    49021 caaaacgatc tcagctgtcg aacgtgcagc cggatcgttt ctctttgctg catcaatcgc
    49081 tcgggtaaac caactcatga ctttccactc ctttcgtaag aattttatgt aggaaatcat
    49141 cttcttataa agaaaagaag cgccttttaa ataagtttcc ttatcaaaag acgctttccg
    49201 cgtggttcca cttttcttca taatcaatga ttatctctga cagctaacgc ctgttatacg
    49261 ttcttggcta ctttgtttcc tataaaacag tttttcctgt tttctgtgtt tcgccaagac
    49321 cactcccaga ggcatttcgg caaccattca ttgatacctt gcaccacccg gtatctctct
    49381 tgtaatgatc agctgcttac ttttttctgt tcatcgtgtt tagttcagta cttgatttaa
    49441 atgtgctaat gatttttcac gaccaagcaa ttcgatcgtt tcaggcaatt ctggaccatg
    49501 catttgtcct gatacagcta cacggattgg catgaaaaga ttttttcctt ttacgccagt
    49561 ttctttttga acagctttga tggcagcttt gacagttgga gcatctacta cttccatttg
    49621 ttctagctgt gctttgaatg cgtggagaac agtaggaact gtttcaccag caagtacttc
    49681 ttttgctgct tcattcaata caggatgttc attgaaaaat agcgtagaca gatcaacgat
    49741 ttctgcagca tagctcattt gaggttgata caggctaacg atcttcttca cccattcgat
    49801 tgtcttttca tctgggtttt ctgctacacg tccatcagcg attagataag gcagacacat
    49861 gtcagtaaat gcatctagat ccattgcttt gatgtaatgg ttgtttaccc attgcagttt
    49921 tttcgcatcg aaagctgcgg gtgatttact taggcgtttt ggatcgaaca ttttgatcaa
    49981 ttcttggtgt tcgaagattt cgtcttcgcc cacaggtgac cagcccaata atgcaataaa
    50041 gttgaacatt gcatctggta gatagcctaa ttcgcgatat tgttcgataa attgcaagat
    50101 tgattcgtca cgtttgctta gttttttacc tgtttcactg ttgatgatca atgtcatatg
    50161 tccgaaggtt ggtggtgtcc agccaaatgc ttcatagatc atcagttgtt ttggtgtatt
    50221 ggcaatatgg tcatctccac gtaaaacgtg agtgattttc atcaaatgat catcgaccgc
    50281 aacagcaaaa ttataagttg gcataccatc gcgtttttgg atgacaaagt cgccaccgat
    50341 attatcggat tcgaacgaaa tagcgccttt caccatatct gtaaatgcgt attctgtatt
    50401 gcgaggcaca cggaaacgta caacagattc tagaccttgt gcttcttttt ctgcttgttc
    50461 ttcaggtgtc aagttcgcac atgttcctgc gtaatgaggc atttctcctc ttgcgcgttg
    50521 tgcttcccgt tctgcttcta attcttcttc tgtgcaatag catttgtatg ctcgattgct
    50581 agcaagcagc tgatcaatca gcggttgata gatttcttta cgttcagatt gacggtaagg
    50641 gccgtattct ccaggatttt caggagattc atcccaattc attcccaacc aagcaaggtt
    50701 ttccaactgg cttttttcgc catcttcgat attacgtttt tggtcagtat cctcaatacg
    50761 aataataaag tctccatcat tgtggcgggc aaacaaataa ttgaataatg cagttcttgc
    50821 atttccaata tgcaggtgcc cagttggact tggtgcatag cgtacgcgta ctttcgtcat
    50881 tttcttcctc ttctttcaaa ctagactttg actaacagca aaaattccgt cagttacggc
    50941 ttctcaaata tcatttattt aaagccactc agcacaaatt ttaccttttc taaggtaaat
    51001 agtaaagcca actggttgaa tttccttacc agttggctga atttcttttt tattttgatt
    51061 gtttagtctt tttctctgtc tcttttttat tgtcgattcc tcgtcctgca tgtgcaggtt
    51121 tagcaaagat catgcgtcca gcagctgttt gaagggcact tgttacaacg acttcgatac
    51181 gttcattcat gtaatgctgt ccatcttcta cgacaaccat cgtgccgtca tcaaggtagg
    51241 ctactccttg ttggcgttct gtcccggctt ttactactag aacattcatc gtttcaccag
    51301 ggatgacgac tggttttacg gcatttgcca acgcattgat attaagaaca ggtacatttt
    51361 ggaattcaga gactttattc aagttatagt cattggttac aatgacacca tctaaaagct
    51421 tagccaattt gatcaatttg ctgtctactt cactgatatc ttcaaagtct ccatcgtaca
    51481 tttcgacaga tataccctct tctttttgca aggcatttag gatatctaat ccgcgacgac
    51541 cacgcacacg tttcaaacta tcccctgagt ccgcgatgta ttgtagttcg taaagaacaa
    51601 aattagggat catcaaggtt ccttctaaga atccagtctt tgtaatatca taaatccgac
    51661 cgtcaatgat cacgcttgta tccaagattt tatatttatg gaaattttct cctgattttc
    51721 gttctaacag ttgactctca acttgactag ctgtttcttg ctcgattttt ttgcttcgtg
    51781 aaccaaaaac tttcttccat tcttcgattc gtgtcgttcc cattctcaat cccagatagc
    51841 ctaagatgat catcagtaaa atcggcaata cactattgac gaatggaatt tccatattgt
    51901 aaagaggggt cgagatcaca acacctaaag tcaatccgat gatcgcaccg atcgcaccga
    51961 agagaagata agtcaagctg acctcactta gtttttgttc tacttgtttg ataatagctg
    52021 agatatgttt ggccaacagc aaagataata aaaagaaaat aagtgcgcct aacaggctgt
    52081 tggtaaatgt attattcagc catccgtttg actgttgacc gattgcctgc caagctgagg
    52141 gtaaaaatga aatacccaag cttgcgccga caatgatcat cagcaaagta atgacacgtt
    52201 tttgcatcgt ttttcctccg tttcgacaat catttattag ttagcggaac acacgcttca
    52261 acgtttcagc caaagttgct actccaacta tctcgattcc ttttggcgct tcccagttac
    52321 ctaagttgtt tttaggtagg tatacttttg tgaagccgag tttttggact tctttgactc
    52381 gttgttcaat atgactgaca cgtctgattt ctccagtcag tccaatttca ccgatgaaac
    52441 attccgttgg ctgtgttcct ttttctttat agcttgaagc aatactgaca gcgatagcta
    52501 aatcaatcgc tggttcattt aattttaccc cacctgcagc ttttaagtaa gcatcttgat
    52561 tctgaagaag caacccagct cttttttcca gcactgccat gatcagagat acccgattga
    52621 aatcaagacc tgttgttgtt cgtttggcat ttccgaacat agtaggtgtg actaatgctt
    52681 ggatctctac caaaattgga cgtgtccctt ccattgccac aacgatagca gagccagttg
    52741 caccgtctag gcgttcttct aagaaaatct gtgaaggatt agcgacttct tccaatccat
    52801 gctcatgcat ttcaaaaatc ccaatctcat tcgttgagcc aaatcggttt tttactgctc
    52861 gcaaaatccg aaaactatga tgttgttcac cttcaaaata taacaccgta tccaccatat
    52921 gttctaacat tcttgggccg gcgatcgagc cttctttggt aacatgaccg acgataaaaa
    52981 tggctatccc atttgtttta gcggttttga gtagttctgc agtggtttct cttacttggc
    53041 tgacacttcc tgccacactt gtgatgtctg gctgcgtcat cgtttggatg gaatcaatga
    53101 taacataatc aggggagata tgttcgatgg cacgactgat ttcgttcata tctgtttcag
    53161 cgtatagata aaattctgtg ttgatacttc cgaggcgttc cgcgcgtaat tttatctgct
    53221 ctgcgctttc ttcgccggat acatataaga cttttccgcc tatggctgct aattgctgag
    53281 aaacttgtaa cagcaaagtg gatttcccga tacctggatc gccaccaatc aagaccatag
    53341 accctggcac cacacctccg cctaacacac ggttcagttc ttctaatttt gttttgatcc
    53401 gaggttcttt tttagggacg acgtctgcga tttttgttgg ttttgctttt tctcctgtca
    53461 aacttgtccg agttctgcgg tccgttgtat cttgttcgat ttcttcgacc atcgtattcc
    53521 attttccgca atttgggcag cgtcccaaga acttaggcga cacatacccg caattttggc
    53581 aaacaaattg tacttttgat ttttttgcca ttgtaccgcc tccttttatc ctaatcatga
    53641 gacattaaat caagcttaag tatctttaac gttttattta ctggaagaac caaatccgcc
    53701 ttgacgttga gcagtcgttt gatcttgatc tgcttttaaa aacggcaaaa agatcccttg
    53761 gccgatacgc tcgccttttt tgatcactac atccttgaaa ccaaagttgg tgaattggaa
    53821 catgatatgg ccttcatttg cctcattatt gtaataatca ctgtcaatca cacctactcc
    53881 gtttgtcaac actaaaaacc gcttcaacgg attgcttgaa cggttagcaa gctgcaaaaa
    53941 ttcatcttct cccatgtaag ctttgattcc agtaggcacg agggttggtt tcaattgttt
    54001 ttcattttct gatacatctt ttttcaatcc atctgcgact gtttccaacc cctttttcca
    54061 gcctgctttc cagatacttg gaatcacgac ctcttctgct gcttcaaaat catagccagc
    54121 agcatgatgt gtagcgcgct gcggtagatt gatattttga tctttatatt tagaaataac
    54181 ttcaaatcct ctttttctca ccggttttcc tccctaaata aactactcct attctaacat
    54241 atccgaggct gtaaaagact ttctttacaa attcttcaag aaattctgct atcttaaagt
    54301 aaaccattaa ttcgttcttt ttccaaacga ctgcaagttg tacaaaaaaa ctatttttta
    54361 catgacttta ctcgtcaaaa caagtagaat agaacgaaat taatgagtgg atatttgatt
    54421 gaggtgtcac atgcgtaaag aaattaaaaa tgcaaaacga attattgtca aagtaggaac
    54481 aagtacattg atttatccca atggaaatat caatcttgcg gcaatcgatc agttagcttt
    54541 tgtcctttct gatcttagaa atcggggaaa ggaagtgatc ttagtctcat caggagctgt
    54601 aggcgttggt atgcataaat tacatttgtc agaacgtccg actttgattc ctgaacagca
    54661 agctgcggcg gctgttggtc aagccgaact gatgaatatc tataatcaaa gattcagtgt
    54721 atataatcaa cagacggcac agatcctcgt gacaagagac gtggtagatt atcctgaaag
    54781 tcgtaaaaat gtgaggaata cattggaaca gcttttgatt atggacacga tccctatcgt
    54841 gaacgaaaac gatactgtag cagtggatga attagatcat ctgacaaaat ttggcgataa
    54901 tgatcagctg tcagccatca tggcaaaact tgtcgcggca gaccttttga tcgtcctttc
    54961 tgatatcgac gggttttatt cagataatcc gactacaaat cccgaggctc agatgtatcg
    55021 ctatatcgat aaaatcgatg aaaagattct ggcacaagca ggtggtgctg gtagcacatt
    55081 tggaacaggc ggtatgcaga gtaaattaaa agccgcttct aggatatttg aagcaaacag
    55141 cgcaatgatt ttagcaaatg gaaaaaatcc ggcaattatt tttgatattc ttgcaggaaa
    55201 agaagtaggt actttattta aggaggactt gaaatgactg acttgatgca attaggacaa
    55261 caggcaaaag atagcgcgca acaactagca cttatgccta caaaccgcaa aaatgagcta
    55321 cttgttcaaa tggcacaaac catcaaaaag aatcaacaag cgatcattga agcaaatcat
    55381 aaagacttag aaaaagcagt cgaaaacaat atcagtgaaa cgatgcaaga ccgcttgcag
    55441 ctgacacctg agcgaatcaa tgccatggct acagagatcg aaaaaattgc ttcgttagac
    55501 gatcctattg gaaaagtaga tgagatgtgg acgaacaccg acggattgag aatcggcaaa
    55561 aagcgcgtgc cactaggcgt gatcggcatc atttacgaat ctcgtccaaa tgtcacgaca
    55621 gatgcagcta gtctagcatt caaatcaggg aatgccgtta tccttcgcgg tggcaaagaa
    55681 gcattcttct cgaatcaatt attagtccaa ttattgcagc aagtactggt cagtgctggc
    55741 gaatctcctt atgccatcca gtttgtcgat gatacttctc atgaaactgc aggacagttg
    55801 atgcgattga cagaatactt agatgtgctg attcctcgag gcggagcaaa attaatccaa
    55861 cgagtcaaag aacaggcaac ggtacctatc attgaaacag gtacaggaaa taatcacgta
    55921 tatatcgata aagaagcaca gttatctatg gcagtgaaca tcatcgtcaa tgcaaaagct
    55981 tcccgtccat ctgtctgtaa cgcggcagaa acattgctga tccattctga gatcgcacct
    56041 tttttcttgc cagctattga aaaagaactg gtagaacacg gggtctcttt acgtgcggat
    56101 gctcgtgcgt tagaatatct agagacggct gctttagcag aagacacaga ttgggatact
    56161 gaatatctag attatatcct tgctgtcaaa gtggtcgatt ctttggatga agcaattgca
    56221 catatcaatc ggcataatac taaacattca gaaacgatcg tgacagatag ttatgcggct
    56281 agtcaacgtt tcttaaatga agtggacgcg gcggctgtct atgtcaatgc ctccactcgt
    56341 tttacagacg gatcggtatt tggttttggt gcagagattg gtatctctac tcaaaagttg
    56401 catgcccgtg gtccaatggg gttaaatgaa ttaacttcca caaaatatat catttatggc
    56461 gatggacaaa tccgcgaata atgtttcgtg tgaaacatta ttcccgcctt ttgccttttt
    56521 tctgtaaagg ttctatccaa ctagaaaggt gtcaatatgt ttgaaatttt aatgattttt
    56581 tttatttacc ttatttctct gaatatcgct gcttttctgg gagtcggtat tttatccctt
    56641 ttctttcaat tcaaaaaacg aagcataggg agtcaacggg aaaaatggtc gcagtatttt
    56701 gacaaaatcg ggcaaaaagg gctggttaca cgcttgcaca ttagttacat ggttgcatta
    56761 tgcttattag cgacaatcaa ttactattct tttttcgatc attcaatcgc ttatacgatt
    56821 acgctactga ttgctgggat ttttcactta tcttataaat atcagctgaa taaaaaccac
    56881 ttgaatcgga ctttccgcta aaaaatatcc gtacgataag tagagtgcct tgaagatcgg
    56941 caaaaaactg ccggactttt tagaggctgt gacaaagatt ttgtcacagc ctctttttcc
    57001 atataaacgg cgttcgccta ccagctgcct ggtaaatctg tttcctcttc gttaagaagc
    57061 gaaaatccgt caaatgtttc gccatcataa taacgaagca gctcatccat ggacaagttc
    57121 atcgtttttt ctttttcttc ttccgctgat tcagccagta gctgttcccg atacacctga
    57181 tacataccag ctattttttg ctgcatcgct tttctagttt caccttccgc attgattatt
    57241 ggcaattctt tgatatagcc acgataaatc aaccgatcat ctgaaaattc aatgggtaat
    57301 tcaacgagtg tcaacccgac ttcctgaaaa tattcttcca gcatctatta tccccccatt
    57361 ctttttctta aatgaatttt agcttaccta ctgaaaattt tccagtttca ttattttgag
    57421 aaaaaaaggg aatgtgacaa aaatcgtgtc acatcccctt tttccgaata aatggtgttc
    57481 ttctgccaac tcctcaatca taagtcacaa tattcaacaa acatgagaaa ctgttttttg
    57541 aaattgttcc ttgtgattca gagttaaacg acagtctcac aacctcttgg taaacggcgt
    57601 tcaatcagct gaccctcggt attagctcaa aaaacggtaa aatatgagaa gctatttttc
    57661 cgttttgttt ctctaatact taggataaaa cgctgatttt acaacctctg ttaaacggtg
    57721 ttcaatcagc tgtccttcgg tattagctca aaaaacggca aaatatgagg aactattttt
    57781 ccgtttgttt ctctaatact caggacaaaa cgctgatttt acaacctcct gataaacggt
    57841 gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg agaagctatt
    57901 tttgtttggc tgttcttatg acttgatgca ggacagcttt ttcacaacct ctgatttctt
    57961 attcttcttc atttgtagca aaataattac ggtgataaat gatacaacat acactgataa
    58021 gaacaatggc acctgcaaaa taaaacgcat agctttcacc aatatgttca ccaatcacac
    58081 ctgcaataaa tggtgctaaa gctccaccga aaaagcggac aaaactatag ctggacgaag
    58141 caacgtttcg ttgcagacct ggtatttcca tagcaatcgt cgtcaacagt gtattgataa
    58201 ttccttggaa aaatcctgct aacacaatgc tgatcggtac aaggactggc atagaagttc
    58261 cagctcctaa taacagcaaa caaataatga ataagacaaa tgctgctaaa attgtccgat
    58321 aagtcgtaaa cttccgctca agagccggtg caacgaaaat cgaagcaata gccagaagca
    58381 caccccagcc aaaaaataca aaaccgactt ctaattcaga atagccaatc aataaaaatg
    58441 gcgcatacgc aagtaaagta aagaatccga aattatataa caatgcgatt agtccaactg
    58501 aacgaagttt atgattgctt aacgctttga caccttccca aaggcctact tttctctttg
    58561 gtttctcagt ttcaggtaaa agtaccgcta gggctataaa accgcaaaac atcaaaaatg
    58621 ctaccccaaa aaatggataa cgccatgaac cactgcccaa gaccccaccg actagagggc
    58681 caacagccat tccgcagcca agtgctgctt catacatcat gattgctttt tctgtgttac
    58741 caactaaaat actgacgatc gttgctaaag cagtagagac aaataaagca ttcccgacac
    58801 cccagccggc acgtaaccca atcagcattc cgatattccc agataatccg ccaagaccag
    58861 cgaatacaac gatgataaac aatcctgtca tcaatgtttt tttcaaaccg atccgactag
    58921 ataagaaacc tgtaaatagc atcaccacac cagtgactaa catgtaacta gtaaataaca
    58981 gggtcgtttc agctggcgta gcgtttaact gtgcagcaat cgtttttaag attgggtcta
    59041 ctagacccac tcccataaaa gcgatcatac aggtagctgc tacaactagc gctgaacttt
    59101 tcccctttgt ttcttcattt gtttgttctt gtatatctat ttgattctcc aatatttcca
    59161 tccctttctc tctattagca tcatacatga tttattttac atgtcaatat gcacatgtaa
    59221 aacgtggaaa ctttactgtg atataataaa gaaaataaga cgagaggtag gatttttatg
    59281 aatactttag cctatatatt attatgcgtg cttttatcca aaccacgttc tgggtatgaa
    59341 ttgaagcagc tgatcacaat tttttgggaa gcccatcaca gtcagatcta tacaacgctt
    59401 gccaagttag accagcaggg ttatgtgacc gttttggaca aagacgagca ctctcaaaaa
    59461 aagatctacc acttgactga tgaaggacgt gctcttgtag aggaatggat caaagaggaa
    59521 actcccgctc ctacgcaaaa agatgaattt ctagcaaaaa tctatgcttt ttcgacgctc
    59581 gataaaacaa cagctactgg actgctattc actagagaac aacagctgaa caaaatcatg
    59641 caaaaaaatc aaaaaaaatt gaatgacctc tcgctagaca agaaacaaga atttggacgt
    59701 tatgttgtga tccagcgcag aattatgctt tgtcagcagg aactccaatg gtgtcagcaa
    59761 gtaagagaac aaatgcagca gttttttgag taagggataa acagaacctt tctagaaata
    59821 tctattgaag agcaaattat cttcaataaa agagcaatac agttggaaaa gaaaaagcga
    59881 tccgaacgac aggccaaaac ctatcgttcg gatcactatt tttctagaac tgcattcaag
    59941 agactatgac aaaaagcatg ccaaggcctt ttttcaaata acagtgttcc atcattcatt
    60001 actttgaatt gcttttagca cacttcttga aagcgggcca acgatgatca actgcaacgg
    60061 taaagcggca atcacattca ttttccaagc attcatataa gcagagaata attgatcagg
    60121 tattccttgt tccatgatca caccgaataa agacatacat gtcaccattc ctaaaaccat
    60181 caagcatgaa atagttaaga tcatttggac cttgctttct ggatttactg gtagtttaaa
    60241 ggcaattttt ttcgccagca caccgatgat gaagacatcc aaaatgaaag ccaccacgaa
    60301 tccaggcact agaccaatca acaaacttga taaatgaaga tcttgatgca gccataaatt
    60361 atacgcgctc atccccaaga ccatcaaaaa acacatcatt gttgtaaaaa ttattccttc
    60421 ttttttattt gtaggcaatc tgtaccactc ctttaaattt gtcacgaaaa tcatcgtagc
    60481 acatttaaaa atttctcgta agtacttttt ttattttctg cccttttatt tcataaactt
    60541 catcagccgt acgctcgaca aagtaagaat catgagaagt aaacaaaatc gttcccggat
    60601 atatttgcat tagcttctct aaagcttcga tcgttgccat atcaataaaa ttagtcggtt
    60661 catctaacag caacacatta cttggcttag taaaaagcaa agcaatcgtc agacgggtcg
    60721 cttctccccc acttaatgta cataacggtt tcgtcaaagt ttcctgagca aaccctaagt
    60781 gattcagcaa actacggaca agcgattctg aaaactctgt ttgtctcatg acaaaacgaa
    60841 tgatggattc ttcagatgtc atttgataac ccttttgctg atatacttga aagcttactt
    60901 tcggagagag aaggattcct tctccttgtt tgcggatatg ttctaataag gttgtcttac
    60961 ctgaaccatt ttcaccgaca atcgcgattt ttttacctaa agaaaactga aaatcaccat
    61021 ttaccagtaa tgttctccca cttctttcca attggacatt ttgtcccatg atgggatagc
    61081 ggctgtggag ttcaagagat tttggtactg gaaaacggat ttgtttcatc tgctgcggct
    61141 tggttgtttc tcctattttt tggagtcttc tctctaatgt tttcgcattc ttttggatgg
    61201 ctttttgtac actgtctttt tgtttagagg aagacaaccg atccagtctg atttgttgct
    61261 ttttcttttt actggacact ttttccaact tttccgcttg ttttttcttt tcttggatcg
    61321 atttttccaa tcgttttttc tctttcatga tcgactctgc ttctcgtagc tgagtctttt
    61381 tctccaattc cttttgagtg agataggcac tataattccc cgaaaactct cggatttctc
    61441 cgtcttttac ttcccagatt ttcgatgcta gctcatctag aaaatagcga tcatgactaa
    61501 caaaaaccag cgtaccataa taataacgaa gctcttcaat cagtcgatca atactttttc
    61561 tatctagatg ggttgttggc tcgtctagaa gcatcccttg ttcataagaa gaaagaagac
    61621 gtatcaactc gatctttcgt tcttcccctc cgctccattt ccgctctgaa gtttcttcca
    61681 gtcttaactg gaaatgactg gctaattcca aatttaggtc ctttgcttca gcgagatagg
    61741 ttaattgagg caggtagttg aaggtgatct ctgtttggat tttcccatga tcgggaaaaa
    61801 gttcaccggc tattagtttg agcaaagtcg atttaccgac accattttct cctatgatcc
    61861 ctatacgatc ttgctgatag acacgaagag aatcaataga tagttctagc tggttgccaa
    61921 agctgacttg tagatttgtt atatttactg ctaaattttc catatgtttt acactcctat
    61981 ttcatgtaaa acaacaatta tcgatactaa aaaagagcag aaaaatctgt ttgcaaaaaa
    62041 actgcatcac atcttttcct actctgaacg aaataactat gactaaatca ccaaatctgt
    62101 catagctttc acctattcta ctgtcgttcg aaaaaatgga catactccat ccatgaaatt
    62161 gttgtttata catgtctttt gaatttattt agttgttatt caaaagttcg aaacgtaatt
    62221 tcatcgatgc agtcattttt ccgacctcct tttttattag gttaccacac acgattcaaa
    62281 aaaagcaata gctactgaat agtaactgaa aaccttttat tcgaacatag tgatcttttc
    62341 tggtgctaaa cgaacagact cgtaaccttc ttctaccgct tttcctattg ataaaataat
    62401 aactggaaca taacggtctt tatccaaacc aaatgcctct gccaactgat ctgcttcgaa
    62461 tccaccaatt ggatttgttt catagccatg atcacgcgca actaacatga attgcatagc
    62521 cgctaagcta gaatcgactt tgactacatc gttcatttct tctcttgaaa aactcttgta
    62581 ataaggaatg atcgcagcta attgttgatc tctgacttct tttggcatct tccctgattc
    62641 gtaagcttgg ttgtagattt cttcgccata ttcgtaacat tgcatatcgc caaaaatcat
    62701 gaccattgca gaagaagtat cattttgtct cgtattgaaa cggatcaaag gacgcaagat
    62761 ttcttttgct tcctcgcttt cagcgaccac aaagcgccat ggtttcatat tgacagaaga
    62821 aggtgcagtc gtcgcttctt tgatcatttc cagcatttct tcgtgtggga ttttgtatgt
    62881 ttcatcatat acgcgaacgg atcttctacc agtcataatt gttgaaaaat catttttttg
    62941 ttccattatt attaacctct ttcgtttctg ttgttatatt gcaaagttgg gtattcaatt
    63001 tatctaaaag tgttagaaat tgtttcattt ctacatcgct gaaagccggg gatagcacgg
    63061 aacctgtatc tttatggtcc atttcacaat gcttgatttt cttctgggct ttttctgtta
    63121 gcgctacata cacttcccgg ttgttctgag gatttctctg acgcgaaaca tagccatttg
    63181 cttccaatac cttcagatga cgagtaactg ctccttgatc aatctgcaac ttttcttgca
    63241 aactgctttg tagcaatgga ttttcttctt tcagtctcat catgatctcg tatctagtaa
    63301 gacttatacc gatattttct tcaaagatct ggacgatttt ttgatcggct aatttgattt
    63361 gatataaaag gtgactaatc tcttctaaag acataatttc ctcctttcta ataattgac
//
LOCUS       DACDSY010000004        47952 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDSY010000004 DACDSY010000000
VERSION     DACDSY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.004"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(38..1189)
                     /locus_tag="IWK44_000243"
     CDS             complement(38..1189)
                     /locus_tag="IWK44_000243"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP9548801.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="IWK44_000244"
     CDS             complement(1202..1639)
                     /locus_tag="IWK44_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9548802.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="IWK44_000245"
     CDS             complement(1654..3420)
                     /locus_tag="IWK44_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP9548803.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="IWK44_000246"
     CDS             3684..4361
                     /locus_tag="IWK44_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9548804.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4528..5490
                     /locus_tag="IWK44_000247"
     CDS             4528..5490
                     /locus_tag="IWK44_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9548805.1"
                     /translation="MIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEAT
                     YHYEKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYE
                     NIFSGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAA
                     MLSLVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLF
                     LSTISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVI
                     IGGDIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5690..6130)
                     /locus_tag="IWK44_000248"
     CDS             complement(5690..6130)
                     /locus_tag="IWK44_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9548806.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6141..8174)
                     /locus_tag="IWK44_000249"
     CDS             complement(6141..8174)
                     /locus_tag="IWK44_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9548807.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8176..9879)
                     /locus_tag="IWK44_000250"
     CDS             complement(8176..9879)
                     /locus_tag="IWK44_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9548808.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9973..10923)
                     /gene="manA"
                     /locus_tag="IWK44_000251"
     CDS             complement(9973..10923)
                     /gene="manA"
                     /locus_tag="IWK44_000251"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP9548809.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10962..11987)
                     /locus_tag="IWK44_000252"
     CDS             complement(10962..11987)
                     /locus_tag="IWK44_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9548810.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12196..13065)
                     /locus_tag="IWK44_000253"
     CDS             complement(12196..13065)
                     /locus_tag="IWK44_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP9548811.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13524..15416)
                     /locus_tag="IWK44_000254"
     CDS             complement(13524..15416)
                     /locus_tag="IWK44_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9548812.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15413..17140)
                     /locus_tag="IWK44_000255"
     CDS             complement(15413..17140)
                     /locus_tag="IWK44_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9548813.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17156..17806)
                     /locus_tag="IWK44_000256"
     CDS             complement(17156..17806)
                     /locus_tag="IWK44_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9548814.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18010..18306
                     /locus_tag="IWK44_000257"
     CDS             18010..18306
                     /locus_tag="IWK44_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9548815.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18478..19098
                     /locus_tag="IWK44_000258"
     CDS             18478..19098
                     /locus_tag="IWK44_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP9548816.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19190..20356)
                     /locus_tag="IWK44_000259"
     CDS             complement(19190..20356)
                     /locus_tag="IWK44_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9548817.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20368..20721)
                     /locus_tag="IWK44_000260"
     CDS             complement(20368..20721)
                     /locus_tag="IWK44_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9548818.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20849..21871
                     /locus_tag="IWK44_000261"
     CDS             20849..21871
                     /locus_tag="IWK44_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP9548819.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(22310..22744)
                     /locus_tag="IWK44_000262"
     CDS             complement(22310..22744)
                     /locus_tag="IWK44_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548820.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22854..24713)
                     /locus_tag="IWK44_000263"
     CDS             complement(22854..24713)
                     /locus_tag="IWK44_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9548821.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24831..25991)
                     /locus_tag="IWK44_000264"
     CDS             complement(24831..25991)
                     /locus_tag="IWK44_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP9548822.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26298..27722)
                     /gene="araA"
                     /locus_tag="IWK44_000265"
     CDS             complement(26298..27722)
                     /gene="araA"
                     /locus_tag="IWK44_000265"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP9548823.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27738..28436)
                     /locus_tag="IWK44_000266"
     CDS             complement(27738..28436)
                     /locus_tag="IWK44_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9548824.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28393..30054)
                     /locus_tag="IWK44_000267"
     CDS             complement(28393..30054)
                     /locus_tag="IWK44_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP9548825.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30272..31672
                     /locus_tag="IWK44_000268"
     CDS             30272..31672
                     /locus_tag="IWK44_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP9548826.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31924..33003)
                     /locus_tag="IWK44_000269"
     CDS             complement(31924..33003)
                     /locus_tag="IWK44_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9548827.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33089..34048)
                     /locus_tag="IWK44_000270"
     CDS             complement(33089..34048)
                     /locus_tag="IWK44_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP9548828.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34060..35583)
                     /locus_tag="IWK44_000271"
     CDS             complement(34060..35583)
                     /locus_tag="IWK44_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9548829.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35593..36156)
                     /locus_tag="IWK44_000272"
     CDS             complement(35593..36156)
                     /locus_tag="IWK44_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9548830.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36162..37025)
                     /locus_tag="IWK44_000273"
     CDS             complement(36162..37025)
                     /locus_tag="IWK44_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9548831.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37039..37935)
                     /locus_tag="IWK44_000274"
     CDS             complement(37039..37935)
                     /locus_tag="IWK44_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9548832.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38110..39381)
                     /locus_tag="IWK44_000275"
     CDS             complement(38110..39381)
                     /locus_tag="IWK44_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9548833.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39371..40888)
                     /locus_tag="IWK44_000276"
     CDS             complement(39371..40888)
                     /locus_tag="IWK44_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9548834.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41086..41976
                     /locus_tag="IWK44_000277"
     CDS             41086..41976
                     /locus_tag="IWK44_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP9548835.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="IWK44_000278"
     CDS             complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="IWK44_000278"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP9548836.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42885..44357)
                     /locus_tag="IWK44_000279"
     CDS             complement(42885..44357)
                     /locus_tag="IWK44_000279"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP9548837.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44994..45356)
                     /locus_tag="IWK44_000280"
     CDS             complement(44994..45356)
                     /locus_tag="IWK44_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9548838.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45579..46331
                     /locus_tag="IWK44_000281"
     CDS             45579..46331
                     /locus_tag="IWK44_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9548839.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46471..47394)
                     /locus_tag="IWK44_000282"
     CDS             complement(46471..47394)
                     /locus_tag="IWK44_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9548840.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47409..47681)
                     /locus_tag="IWK44_000283"
     CDS             complement(47409..47681)
                     /locus_tag="IWK44_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548841.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13980 a         9954 c         8268 g        15750 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt ttttgatcat aaacttgctt atacagaatt cgatgaactg
     4501 aatcataact atgaagagat gcaacgtttg attgaaacgt tgaaagaaga tgagaatgag
     4561 aaagaatttg tggaaagttt agagaaaagc aacaaactga tcgaagcaat cttacatgca
     4621 aaaaacacag ggaatgtcca gcaaatggtc gaagcaactt atcattatga aaaagatata
     4681 ctggatcgac tgatttctgg tcaaagacaa ggaattccaa tcattgaaca acaaaaacgt
     4741 gtcgagttgc tccgttatat gaaagaacat cagatccaaa gatattctat ttttgacttg
     4801 ccagcgcatc tccctttagc aaattactac gaaaatattt tttcaggaat gatttctagt
     4861 tttttgattt tatgtataac cgccttgttt ctttcttcca tcatctctta tgaaaaaaga
     4921 aagcaggtaa tctctctagt caatctatta ccggatagta tggtaaagaa acatagcatc
     4981 cgttttacca tttattatgg tgcagctatg ctaagtctcg tgatgccatt tttgatcgtc
     5041 tctatactag taataatcaa aaatggtttg ggagacttcc gttatcctgt aggaacgatc
     5101 attggtcagg agatacgaat ccttccgatg tatgagtatc ttttccagag ctttctgttc
     5161 ctgttgttgt gggtgctctt tttaagtaca atcagttttc ttctatctgc tttatttgaa
     5221 cattctctag tcaatctttt aggtacactt ctttgtcttt tcttagcaga gtatcgcttg
     5281 ttttcttcga ttggatggat agaatcaatc agtcattact tgccaacttc ttatgttgat
     5341 tttcaaaatg tgatcatagg aggagatatt ttctcgccac tagcttcaga acaggttact
     5401 ttcatgaatg gtatcttgac acttggtata tggagtatcg ttttattatt cataggtatg
     5461 ggcactatct atataaaaaa gagctattaa aacacaaaaa taataggggc ggtgacaaaa
     5521 accttgtcac cgtcccctat ttcaaataaa cggttttcta gaataaatag ttttcaaaag
     5581 cttgcatccg cagtaataag aacctcccaa caaaaatagc tcctcttatt tttatcttgg
     5641 ttgaagttat tacttgatgc aaggcagctt tttttactgt gaacattggt tatatatgtc
     5701 catgttcaaa aatcatttgg acttcttcaa tggttttcgc atcactgagc gccattactt
     5761 ggtcgatgtc tgaacagaaa aaggctaaat cttggagcac cgctaactga tgttcttttt
     5821 ttaaagccac tacaaataag agtgtcgcga tttttggttc ctcttttgtt ccaaagtcca
     5881 caccatcagg cacttggact aaatagattc cttcttctac tacttcttca tctttgtcag
     5941 tcccatgtgg caaggctacg aaattcccga tatagacact cgctttttct tgtctttcga
     6001 tcattgattg gatatatgcc ggtttgattc ccccttgttg ggtcatccac tggccgatct
     6061 cggtcaacgc ttctttccaa ttttgatagg attgatcgag caatattttt tctgctggta
     6121 gctgtatcat cttttcacct tcatttcttt atttcttcaa tcaggatttt cgctaacaac
     6181 tgataaacga tttctttatt tcctgagcgg aagatctcgg tgttcaagtc attcatgatg
     6241 attgctccac tgatctttcc taaaagaatc ccgtcttttt cttggattgg cgaaggcgcc
     6301 agcataacta acatacggga caaatcgact ggctgtctat ccatcccttc aatggtcaac
     6361 ggatgtttca aattgaaaat acaaaagatc ggtttagaaa tcaatgagct ggatgtatgg
     6421 aataacccca tatttgtatg aggaataccg ataggtgctt gttcatatcg cttaagcagc
     6481 cggcgatgaa ccacctcttc atccgttact agtccttcag gaaaataagg aaataagctg
     6541 cgaatcgttt cggaaatcgt ctcaggatta tccagttctt ggataaaaaa acgaccgagt
     6601 aattgattga tttgatccat gaaatgcatt gtttcctcat aagtatcttc tatcatccac
     6661 tcgttattgc tgttatacgt aattttttgc ctttttttct tctcaatatg cgaaaaagct
     6721 tcttttagct gcttgacttc atcttctagc agtagcgggg agactaaaat atattttcca
     6781 ccgtatcctg gtaaaataga tgtagaaata acaacatcat attcttcttg taaatcgatt
     6841 ttttttaaat cagcaatccg atagaaatga atttgttgga tgaagggaaa ataccttttc
     6901 aatcgtagtt ccaacatgct agtagaagct aatccacttg gcgaaaatcc tgcaatactg
     6961 atattgcttt ctttcggact tcgttccaat gaattcgcaa aatgaaggac catataagcg
     7021 atctcttctt cggataattt tttttcttga aaaatccgct ctactgcttt acggattgca
     7081 ttcgcaactt cctcgtattg aaccatgatt ttttctaaaa tcggattggt cagtgtctct
     7141 tcttgtaaaa cggctcttga aaacacacca gataaatgag tcaacagcat tttgtacaaa
     7201 gtttcatctt cataaaaatg gacattcgtc tcttcactta cttcttgaat cagttgtttt
     7261 actaaataag caagctgtgt atcgaacgtc tcttcaaaga aattctgatc aaatgagttc
     7321 gtgaagtcat ttaataatcc tgcaaaaaag acgatctcat ttactggata aagttgtttt
     7381 gtctcagaag cgatccgtga aaacaatcgt ttagctgtct gtagtaattc tttgtttgcc
     7441 agactattat atgtctcatt tgtcagtaaa tgatttttag tcacacgctc catcgtcaat
     7501 gtcagcatca aaaccaaatg ttcaagcttt ctatcactta gattggtctt tttttggatc
     7561 aattcttccc cgattacttt gcgtgcaaga gatagatgtt gcggatcaac gaatcgaaaa
     7621 aaataattca gtgatgcatc gaaagaaata ctatgaaaaa gttcatattc attgatttct
     7681 aattctaata cattcgctgt cagaagccgg cggtattttt ccggtccttc gatctcgtat
     7741 ccctggttcc gaatgatttc caaaggcagt tggcgaatac tttcttccaa ctgtttgata
     7801 tctgcataaa aagtggtatt actgatgcga tatgattcca tgaaatgagc aagactcaac
     7861 ggctctgtcg tcagtaacaa ctcggctaaa atcgcgtgtt gtcgtttagc agtggataag
     7921 tcattacttt tcccatctgt attttctttt aattgctcta atacttcttc tgatgcctca
     7981 atgacaaatg agcctctagc tgcttttttc aaagaagcat ttaccgattt caatgagact
     8041 tcaagattat ctaattcgcg gtaaatagtc cgtttactga ccttcaataa attcatcatt
     8101 ttttggatag atacaggtga cgggctactg agaaattctg ctaataattg tttttctctg
     8161 ggcgataaat acatcttata tttctccctt cacgagtttt tcgtattcat tcgtagctac
     8221 ccaatgttca actagaacta gccgagggat cttttcttct agctgttcag ttacagctag
     8281 ttctctttgt gcgatataaa tcgtttcttc ttggattttg attttttcta ttgattcttt
     8341 tgagataaca aactgttttt tcgctttttt tgctaattga cttaaaatcg tcatgcccat
     8401 agtttgtgag ccgcgtctgt tgtttttata caaaaataca atattagcag gcgacttttt
     8461 ttgttctgtg atatctgcta tctctttttt ttcttcccgc tttggaaatt ggatcgcttc
     8521 ttttattcgt gacagcaaga tatctgtttc caaaaagtga tccagtacat agatttgact
     8581 ttgaggtact ttttctcttg caagacccgc tagttcatgt tgcacgataa ccagctgatt
     8641 tacttggtta ttcaattcat agatagagct gtatccgaca gaaagatcga ttttttctgc
     8701 tgctaatttc tttttcagca gggcggctcc catagcactt gatcccatac ctgagtcgca
     8761 agcaaaaatg acggaatgaa tgatagatgg ttccaagatt tctccccttt ctttgaccgt
     8821 tttgttctct ttttctttat ttggtaattt caatagtatc gctgcacaaa tgaaggtcac
     8881 aaatgtactg attgctatcc ctatacttac cccagctagt tgtggataag gcgcattggc
     8941 aaaaatcacg agtagtgacc caggcgatac aggtgcagct aatcccgtat ctaaccattg
     9001 aaatatcaac gtgccgcttg ccccaccagc cattacagct aaaaatagat acggtcgcag
     9061 caagacaaat ggaaaataga tttcgtgaat cccgccaatc gcatggatca tcaatgctcc
     9121 actcgcattt actttttcct gtttttctgc aaaacaaagg atagaaagta agatccctaa
     9181 accaggtcca ggatttgcct ccatcaaaaa taaggaagag tacccgactg tttgtgagaa
     9241 ttcgattcct aaaggtgtca atacaccatg attgattgca ttattgaaaa atagtacttt
     9301 tagaggttct aagatcagac tgacaagtgg taacagattc attgtgacta gccaagacac
     9361 aacggaggcg agcaacaagt gaacactatt taaaactggt tcaataaaaa cgattccaat
     9421 tatacaaata acgctaccag tcagtcctac tagtaggttg ctgacaagca tttcatatcc
     9481 agccttgaat ttcacgtgga atctagaaac aaattgttca tatacgatgc cggcaagcgg
     9541 cccgataatc atcgctccta cgatttgtgg agcattggaa cgagcgatca accctaaaga
     9601 agcaattgca ccggcgactg cccctttttg tccccctacc gatttcccgc ctgaataact
     9661 gatcaagatg ggtaaaagca gctggatcat ccaatggcat acctcttcta atgtggtgtg
     9721 caaatcgcca gacacacctt ttgttgctaa tgacaagatt ccccaagcga taaacagact
     9781 tagatttggc atgatcattt catttaagtg atgtccagct tttctcagtt gtttcataga
     9841 cacgcgactg ctgttgtttg ctttccattt gtattccatc tttttcacca tacttctatt
     9901 tattgatgga cggattttat gtagaaaaag cagcaaacga aaaaccgtct gctgctttca
     9961 gctatctggg aactatttgc ctggttcagt cgcaatgatt gctagttcac cttggaccgc
    10021 ccactcagaa acgccatttg gaataataca gcttgttcct ttttctaatg gatatgtttt
    10081 tccgtcaacg acaaggtctc ctgctccttc gattaccgtc atcaatgtat aaggggcttg
    10141 ttttttgaaa gatgtgattc ctttaatatc ccactcatat acattgaaaa actcagtttc
    10201 taaataagtg acgatcgatg aattcccttt tcggacttct ttgatttgta attctggtgt
    10261 tttcgcaggg acagttgtta catcgatcga ttgttggata tgcagctctc ttgtttgtcc
    10321 ttgcgcatct tttctgtcgt agtcatacac gcggtaagtc gtgtcagaac tttgctgtgt
    10381 ttctaaaatc atgattcctt taccaatagc atggatcgtt ccgcttggta cataaaagaa
    10441 atcacctttt ttaactggta ctttcttcaa aaggtcatcc cagcgcccat ctttgatcat
    10501 ctcagctagt tcttctcttg tctttgcatg atgaccatag atgatttctg caccaggttc
    10561 agcatcgatg atataccagc attcagtttt tcctaattca ccttcatgtt tcatcccata
    10621 ggcatcatct ggatgtactt gtacagatag atcatcttct gcgtctaaga tcttgatcaa
    10681 taatggaaac actggttctg aaggatggcc aaacaattcc ttgtgttctg accatagttc
    10741 atctaatctt ttccctttga attcgccatt ttctactaca ctgacaccat gaggatgcgc
    10801 actgatcgcc caatcttctc cgattttatc gttagggata tcaaaaccaa agactgaacg
    10861 taaacgactt cctccccaaa ttttttcttg aaaaactggt tttaaaaaca taggttcctg
    10921 cataattcaa actcctctca tttttcatag ctattatagc attatttcgg taataccgct
    10981 ttctctttca aaaagttttc aacaatttca tctcgacata tgagatactc cgcacgatga
    11041 tcgacaggat gtacgcgatt tgacaaaaat atcaacgctt cttgtgtttt tatatcaatc
    11101 aagaaaaacg tacctgtata accagtatga aaaagaatcg catgaccgtc ttctacatcc
    11161 ttttttaagt cccatcctaa agaacgatag ccattttttt tcggtgtttg atcttgcagt
    11221 aaagaatcaa tcgtctcttc ttctaaaaaa cgaccattac ggatttttcc cttgttcaaa
    11281 tacatttgga caaaagtaat gctgtctttc aaatcaataa agagacccgc gttacctgca
    11341 tgttcagcca aaactcttgc tttcggatca tgagtttgtc cacgcaaaac ctcacccgaa
    11401 ggcagttgtt ctgtgggaac gatttttttg gaaggaaaag gaagaaataa gctgttcatc
    11461 attcctaatg gttccaatac ttgttcttga aagatttcca ctgcagtttc ttggtaaatt
    11521 tcctctagca tcaacccaag aagaatcgta ccggcatctg tatattttac ttcttttcct
    11581 agttgctcac ctggttctag cttcaaataa gcggcgatca attcttcctg agatagctta
    11641 tctctgtttg gtatccatgt tttgatatca gaagtatgtg tcagtaaatg acgcaaagtg
    11701 attgtctgat tttcaaattc aggaagatat ttatgaagcg gttgatcgag ttctatcttt
    11761 ccatcttcta atagacgtaa aactaatgaa gtggttgcaa cgactttcgt cagtgaggct
    11821 gcatcaaaca acagatcttc tgtcatcagc tgcttttcag gaacgacttg ggcaaagccg
    11881 gctgtaaatg tttctacttg attttctttt atgaaggcga ctgctacacc tggataaaat
    11941 ccttcttcca tctttttcaa aataagctgt ttcgtgtgtg gatacatctt tttctcctgc
    12001 ttttctatca gattcttaca gtccaagctt aaagcttaat ctatgaggaa tcaacctttt
    12061 ttacacaaaa aaactgcgcg aaaaaccgcg cagtttcccc cggtatacta ttttctcttt
    12121 ctatctctag aataagcgat agtatgaccg ttcataaaat gacgagacat gcgacaatcg
    12181 ttaagaaaga aaaagttaag cgtcgcatcg aatgtcagtt ttattctttt cttttggctc
    12241 actgacgaac caccattcga ttcctatagc atcatagatt gtgagtactg tgtgcttttt
    12301 gtctaagaag aaatcaactg atagttgctg caagtgagca atcagtcctt ccatgctttc
    12361 agtatcgatc atgaatttca gaaaatcaag tccaatcact tcatctgtag gcaaatcaat
    12421 tgcgcctccg ctagcttctg tcaaaccaac atggaaatct ccttcattca atacatggat
    12481 gccctcttct tcattatgta ccttcaaacc aagtacatga ttaagaaatt ggcgttcttt
    12541 tgttaagtta gtaacattta aatggatttt ttcaaaataa gaacctgttg atagtttact
    12601 gaatttttct tcagattttc ttaataatgc ttcttgatcc attgtttctg gaatttctct
    12661 ttggtcgttc ttattctctt catggtaaat ttccaattca ttctcttctg ggtctacgag
    12721 caaaatcccc atacgtccat gatcttctaa agcttctttg attggataat tcattttacg
    12781 gatacgacaa aggatatctg ccatttcttc tgtgctaggg ataatcaatg aaaaacggtt
    12841 cagctttttg atcaccccca tgtgatcatt cgctctagga ctttcttcca gcagcaacat
    12901 ttcacttcca gcttcttctg ttcctaaaat ggctaattcg ttttcttctc ttttcaaatc
    12961 aaagccaaca acgttcttgt aaaaatcaat cattttatct cggtctttca cccgaatggc
    13021 tacacttttt aaataggtgg cagcccccaa ttgaaaatct ttcatatttc ttgctcccct
    13081 ctacgtcaag ctttatttta acgatggatt cttcttcttt caagcaataa ctttgatttt
    13141 gttaaaaaaa atgtgagaaa attcgcattt tgcttgtttt ttgtaaatta atgagtaaaa
    13201 actcttataa aaagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
    13261 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
    13321 gaacaccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca
    13381 aaacggaaaa atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg
    13441 attgaacacc gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa
    13501 aaaaataata aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa
    13561 agatctgcat agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc
    13621 gaaccatgat tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca
    13681 acaacgaaac ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct
    13741 gttcgtgtat ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag
    13801 aatgcccgcg caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg
    13861 tttaatacag tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct
    13921 ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca
    13981 tagatacttc ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat
    14041 tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca
    14101 tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca
    14161 atagcgacca tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct
    14221 ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct
    14281 ggaaggccag atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg
    14341 gctgaagcaa ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga
    14401 gagaactgtg ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt
    14461 gcaactttta ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc
    14521 atcaacggca tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc
    14581 tgattttctt tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg
    14641 gtatgaccac catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga
    14701 tctgcgaaaa acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg
    14761 atcatcaata acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc
    14821 ccaatcaatg tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg
    14881 atgttatcca tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa
    14941 taggaaatcg gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt
    15001 cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa
    15061 tacatcacga ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt
    15121 gttacttgtt ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct
    15181 gtggttgctt tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct
    15241 agtacaaata cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt
    15301 accgttcccc agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca
    15361 ccatgtcctg gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta
    15421 agtcctcctc tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata
    15481 gttcttcatg tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg
    15541 catccattac tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg
    15601 ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat
    15661 caaacacata gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt
    15721 gtccaccaga gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct
    15781 ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg
    15841 catccggctt accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt
    15901 gaggaacaaa gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc
    15961 catttatttg gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg
    16021 tcgttttacc tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga
    16081 aatcaatatc ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga
    16141 aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga
    16201 taccgatttt ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca
    16261 ccatgataaa aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca
    16321 taaatgcaat cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc
    16381 aagtgattgc tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga
    16441 cgatcgtatt aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat
    16501 tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc
    16561 gaaataccag gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa
    16621 agtacatgat tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg
    16681 tcaactgatg atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat
    16741 tgatcatcat acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg
    16801 ttcttgtgat caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg
    16861 attttttata gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa
    16921 aaaatgtatt tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac
    16981 ctgtatgcca aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg
    17041 taagtgtcgg caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa
    17101 ttgctgaagt caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt
    17161 tatatactcc gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta
    17221 ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag
    17281 tattcatcac taattggatc agtaattcca attcatgatc attttttact ttcagcttcg
    17341 tccgatcgat caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt
    17401 gaggcgtttt ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt
    17461 tcataaacaa gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt
    17521 tagaaaaata ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta
    17581 acatatcttt agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt
    17641 tatcttcaaa atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag
    17701 caatcgaagc ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc
    17761 ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg atcctccttt
    17821 ctttcaagat gacacattgt catatgacgc tgtgtcatta taacacctcc tacctgtttt
    17881 gcaagacatt tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg
    17941 aatccttcca tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg
    18001 atgagataaa tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct
    18061 gacagcatcg ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac
    18121 tcgctttcta tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa
    18181 ctttattata ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact
    18241 gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt
    18301 gcttgaatat tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc
    18361 ttttataccc tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag
    18421 ccttttcttt ttctcatagt tataacaatc atagcaataa aaggagaagg taagcatatg
    18481 gcatctgttt tagtagtcaa aggtcatccc ctaactgcag aagaatcgcg cacagttaag
    18541 gcattgacgt cttttttaac aagttataaa gaaaatcatc cagatgatga agtgactgtt
    18601 ttggaacttt atcgcgatga tattccggaa atcgatgaag aattattatc aggttgggaa
    18661 gcattacgtg ctggtgcaga attcacttcc ttatcagaaa gtcaacaaca aaaaattgct
    18721 cgtttcaatg aattgactga tcagttcctt gctgctgata aaatcgttat cgcaaacgcc
    18781 ttatggaatc taaatatccc aacaaaatta aaagcttggt tcgataccgt caatgtagct
    18841 ggaaaaacgt tccgctatac agaaaacggg ccagaaggat tagtgacagg taaaaaagca
    18901 ctgcacatcc aatctaatgg cggtgtatac aacggacaag actttgcctc tcaatatgta
    18961 aaaggtattt taaactttgt aggcatcgat caagtagatc agctattcat cgaaggaatc
    19021 gattatgacc ctgaccgtgc agatgagttg atgcaaaatg cattagataa agctgctgct
    19081 ttaggtaaat cattttaaaa attgagataa atgcatacaa aagagacaga ggaaaaaatc
    19141 ttcaaagcag catttgtacc ttggatattt tcccatctgt ctctttttgt tattctttct
    19201 gctttttcaa cggattttct tcataaagag aagagattgt tgtgtaccat tcaaaaacat
    19261 gagtaatcac tactttagct gctgcataaa caggaatccc caagatcaca ccgatgattc
    19321 cgaacaattt cccagaagtc agcagaacta ataaaatcgt tactggatgg attgctaact
    19381 gattccctag tactaatgga gagatcaagc gtccttctat tgtctgctct actgcaaaaa
    19441 cgatcagtac tttgatgatc atgaatggac ctgctacaat ggctaaaaag atagccggaa
    19501 tcatcgctaa aaatgatcct aagtaaggga tcaggttcaa aaaacccgca ataatcccca
    19561 acgtgacagc atactctaaa ccaatgaccg caaaaccaat cataaacatg attgccacag
    19621 caaaagccac agttagttgt cctcttatat aagaagaaac ttgactattc atttcactca
    19681 gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta
    19741 attgtttacc gtctttcaat agataaaata agataaacgg catcgtcagc agagccacta
    19801 aaatagtcgc taccgcgccg acaaaacttc caagactctg aactgtactc ttagatatat
    19861 cttggatcat atcgcctgcc gaattcatga acttatcacc tgctgtttcc aactgttcgc
    19921 gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa tttattatca atcgtatcta
    19981 catattgagg gaagttactg atgaagctca ctgtttgttc ctgaatctta gggatgatca
    20041 ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa
    20101 gccttggtac ttttcgcttt tcaaagtaat caactaccgg attcatcaag taatacaaga
    20161 ttcctgctaa aatgataggc agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca
    20221 aatatgaaac ttttgtaaat aaaaagacga tcagcaatat taacaataca acaagtaagg
    20281 ctgtgactac ttgattattt aaaaaccagc gccaaaacca cgataccggt ctgttcttct
    20341 tattttttcc ttccataatt cctcctgtta gttataaaaa atttggcttc ccaaaccaat
    20401 agcaggcagt ctatgcggat gaagatagat tccatttgca atttgtcctt cttctggaca
    20461 agcatcaaaa atcaatgata cgtgagctac tgtctctaaa ttcccattgg ccatcggacc
    20521 gacataatcg atcgtatata cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga
    20581 aaaaggttcg tcttttgata cttcttgaat caccgaatat tttcgtaagg cagctgttgc
    20641 tgttttatcg aacaaaatca gcatgggttc ttccttgttt attgcttctg tcccaatttc
    20701 tgtaatcttt gcctgaatca ttgcactctc tcctttaatt gctttcaaaa acagtataac
    20761 acattctcat tctttagccg tgtggaataa ttatgtgata gaataaaaac gaaaactatt
    20821 tcattacaat taggaggaat cagctcatat gatcgaaaag ataattttag gaacctatac
    20881 gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat acagaaaaag aagaattagc
    20941 caacctttca ttagctgttg aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg
    21001 caatctttac acagttacta gtgtggatgg attaggtggt gccggtgcgt acgataagga
    21061 ttttcatttt ttgaatgcgg taacagagtc tggcgctcct ttgtgttatg tagcggttga
    21121 tgaagcacgt caattactat acggggcgaa ctatcataaa ggtgaagtaa atgtctacca
    21181 tatattagga gatggcgaac tgaaagcagc agattcaata ttccatcaag aagcgacagg
    21241 tcctcataaa aaccaagatc atgcgcatgt ccactatacg gatctgactc ctgaccaacg
    21301 attagtcgta tgtgatcttg gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg
    21361 caaattgaat cttgtaacta ccttcactgc agagccagga acaggccctc gccatcttgt
    21421 ctttcatcca aatggacaat tcgcttattt atttggtgaa ttagacagca cagtccgcgt
    21481 gctttcttat aataaagaaa caggtgcttt tgaagaatta cagaaaatat cgaccttgcc
    21541 agaagaattc gacggtgaaa atggaggggc agctatccgc atttcaaatg atggcaagtt
    21601 cctttatgct tctaaccgag gacataactc gattgctgta ttcgcagttt ttgaagatgg
    21661 ttcccacatt caaaacaagc aaatcatctc aacagaaggg gattttcccc gcgatttcgc
    21721 actagatcca agcaataatt ttgtcgtgtg tgccaatcag aacaccgata atctgacatt
    21781 atatcgccgt catcctgaaa caggattact ggaattactg aaaaaagatg tctatgtacc
    21841 agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca
    21901 agacgtatta gacagactac gggaagaact aaatattcct ttctttgatg ggaaaatcga
    21961 agataaagag tatagcgaag aggaatatca gaaactcaaa gccgatttac agaactactt
    22021 tgaacaatat gtacagaatg tagaaaacta atcagctttc catactatct gaaaagaaca
    22081 tcaggacaag acttttccgt ctatcctgat gttcttttct acaaataaaa aaccgccgag
    22141 atggttagac tcgacggaaa aggagtattt actcgatttt gagtaagtac aaggttgttg
    22201 ttggttatag gtatgtcatt atcataaagg attttgatca ctctatcata aaggtaatat
    22261 tacatttata ttagataact ctagttttta ttgttatcaa agatttcttt tattttttca
    22321 acaacaaatt agtcattttt tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt
    22381 ttgcttcaga tgtattgaat agatcttcat ctacccctga ttcaagatcg atactttgga
    22441 tcatttcttc acagcctgct acatatgaaa gataagcttt ttcaaaattt ttatgaatgc
    22501 ccattgcttt tgctggtgga cgcaaagtac tgactttctt aagcattgcc cgatattttt
    22561 ttgttccttc ttcaaataac aggatgatct cttcaagagt cgatttgtcc agttcacttg
    22621 tttttccttc atcaattgcg tttctgatca cttcatattt cggatgcatt ccttctccga
    22681 ttgcttctgt ttccttgacg atttgatcga tggactgcac ataatagcca atattcggta
    22741 acatgttcgt tttcctactt tctccattgt tgttattatt tattctactt gtttttatcc
    22801 tcaaatgaca agtacttgaa aaaatttaat ggtttctata cagctcgcca ttcttattcg
    22861 tgctcaaaga aaccatctgc taaactatat tgtttccctg cgacaatgtc atattggatc
    22921 aattgatatt cgtcgtaaat ctcttgttgc gagcgattga attgcgcttc tttataccac
    22981 tgtccgtttt gatagtacag ttctcgttca aacgccggaa gttcctgttc taatgataaa
    23041 agcagctgat aaaagccatt tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa
    23101 taaaacggac tggtgattgc atcgtgagtc gtttgctttt ctaattctcc tttactgtcg
    23161 aacataagaa attgtgtttc atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct
    23221 gaatagatcc ctggaagatg atccccccaa aaaacaacta aagtccttct agaaagattc
    23281 ttcaattctt ccgtgaactg cttcagtgcc gtgctgctat aggaaatatc ttgcagataa
    23341 ttctctaagt caagtgtccc acttccatct gaagtttttg caggatagtc tagattatca
    23401 tactttccat cgtagggcat atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt
    23461 ttttcttcct tgagaagtgt cattacttct ttgtaagctg actcatcaga aatatatgga
    23521 ttattctcta tcgtatccgt ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct
    23581 aatgtctgat aaacatcctc tcttttatac atcgaagtat tgtatggatg gatcgctgtc
    23641 gtctggtatg attgtgcgtt taacgctgaa acgatagaag gaagctgatc catttttggt
    23701 acaagcatcg tgtaaggggt ggtgagttgc gcattaaata acgccataga aaaaccagtc
    23761 aatgcttcaa attcgatatt tgctgttcca ccgccatagt tttgagaaag catattgcca
    23821 ctatatgttt gatcagctac ttcataataa tctgccaatg gctcaccaga tacttctacg
    23881 ccgtttaatc gactaggatc tgagaaactt tcactcatca cgaacacgat gttcggcgac
    23941 tcttcctcta cagttttatt tttttcatcg gctagcttct ggtatttttc agtgatttct
    24001 ttgatctttt ctttcgaata tccttcaggt tcatccattg gttctacctt cagattgtac
    24061 aaaaacccac cgataaaacc agtgttatag tagttcattt tctggctgta aggtatccat
    24121 agtgccgttt tattatatgc ttttcgcaac aaattatcgg gattgttgaa attactgata
    24181 taccctagca gtccaattgc cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt
    24241 ccttttttca ggcttcgaaa catgtaccag caaaacaatc ctatcacggt tcctgcggca
    24301 gctaagatca ctgtaaacag catagtgccg gtcatatctt tcagcaatcc gatttcagtg
    24361 atcatcttca aatcgtctgg ataaatcggt tcttgccgat aactcatttt catataattt
    24421 gcaaaaccta atacaccgat ggctacgaca tacgtaaggg aacctaaagg gacagatcct
    24481 gctagtgcaa tcagaaaaat aagaataatc agcaagacga gacaggctaa gaagaatttt
    24541 tccgtatgcc aagaaaaagc aaatttcaac gcaaggtcta cggatagatc attttgacac
    24601 cactgcaaaa ataaattact aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt
    24661 ccttttaaaa tctttttctg tttggtcgaa agtctgctta tatatctttt caaatgcttg
    24721 ctccttcatt tctatcaata aaagaatgtt gcttctctta ttttagttgt acgaaattcg
    24781 aaagtaaaga acggaaagaa gaattaatga aatccaaata ttctctttct ttatggcctt
    24841 tctggtttct ttaataagcg gacctttcgt actccagaaa ttttttctag ctgttgtttc
    24901 acttgattga gctgttcaaa atcagtactg tctatgtcaa tcaatatgta gacatgctca
    24961 ttcttatggt tacgcgatat atctgcgatc gctagctcgt tctcattcag cagagaaaat
    25021 atctctgcta atatattggt tttcttttga taaaatatag tgaatcgaaa cggcgcttgg
    25081 aaaaacattc gtgcggatgg ataattgacc gcttctctaa ccgtaccgaa caaaagataa
    25141 tcttttaagg cttcaactgc caaacgtccg ctgtctgcca aagcttcctg agtcgatccc
    25201 cctagatgag gcaacataag tatacggtca ttttctaaga actcttcttc tggaaaatca
    25261 gtcaagtatt tagccagatg ttgtttttcc aatgcttgca taacagcata tttatcaaca
    25321 atttcacttc ttcctacatt gataagtaca gcatcttttt tcattttttc tatattcgcc
    25381 tgatcgatca atccttttgt atcttctgtc aacggcagca tgactacgat gtaatccgag
    25441 gtagaaagaa ctgtttctaa atctgcttgc cgaacataat ccaactgggc atcgcggata
    25501 gaataaccca agacatccat ccctaagcta tagcaactta gcgctacttt tgtgcctatc
    25561 gctccaagac caagaagacc gatggttttg ccttgcaatt ctcgtcccac ataagcactt
    25621 cttttgtttt ctgcctgttc taaaatatta ggacctgtta gtgtttgtac catacgactt
    25681 gcttcgatta tcggacggga agataagagc aaacagcaca aaacaagctc ttttactgca
    25741 ttagcattta cacctggtgt attcatgacg atcgtcccgt tttcagaagc tttttcaacg
    25801 ttgatcgtgt tcaccccaac accagctctt gaaattgcta gcaaatctgg tgtaagccat
    25861 tcatctttca ctttcgtact acgaataact attccttcgg ctggcaaatc atctattatt
    25921 ttgaaatatt ccccttgtaa cttttctatc tcttgttctg gaatagcatc aatcaaatga
    25981 acgtgataca taaaaccccc tccttactaa caagtatctc gaaatttctg caattagaca
    26041 actgtttacc atttgacttc ctagaagcaa aaagagcagt tagagcagag taggctgagt
    26101 gataaaaata aggctatgac aagttactgc cataagcctt attttcgaga gcaaaatatt
    26161 gagggtgaga tatcttgata aatggtgttc cgttagctga cattgataat aagactaaat
    26221 ttcaaaaaag ttgaaaagca aatttcgtca atctcttctt atttcttaaa actaagtggc
    26281 tttttcacaa cctcttttta tctaataacc actgtttcca actcaactaa ttttgcccac
    26341 gtttcaattt gatctgttgt tagatttagc gagacaacgg tatgatgacc gccacctgcc
    26401 tgaatccatg aatggacacc ttcatggaaa ttcggtttag ttttccataa cacacgtgca
    26461 acaggtaaat taggtgcttc agtcgttggt tcaaaggctt ctacttcatt gatcagcagt
    26521 ttataatgcg taccgaaatc tgccatcgat acgactaccc cttctcctgc tttcccatca
    26581 aatacaagac gcgccggatc ttctctatct cccattgata aaggagaaac aacgattctt
    26641 ggtttcgtcg cagcaagcgt cggatctact tccatcatat gggattccaa gattgcttct
    26701 tggccactag ctagctcgta agtataatct tccatgaatc ctgtatcttt ccctcgagcc
    26761 atgattttca gcaaccgatc aatcgctgca gttttccagt cgccttcccc agcaaaaccg
    26821 tatccttctg ccatcaaacg ttgtacagct agtccaggta gttgtttcat tccatacagg
    26881 tcttcaaaat tagtcgtaaa agcgttatag cctccttctt ctaagaaacg gcgaataccg
    26941 atttcctgct gagcttgtac ttttacatgt tcctcccatg tcttttcttc ataatcacca
    27001 tagtcgaagg tatacaattc cttgtactct tcaaataatt gatcgacttc ttcgtctgat
    27061 acgcgatcga ttacttgtac aagatctccg ataccaaaat aatcaacagt ccaaccaaat
    27121 tgaatctgtg cttccacttt gtctccttct gtgacaccca cgtttctcat gttgtcacca
    27181 aaacgagcca cttttattcc aaaactttca ttataggcta ctgcaacatc catccaatca
    27241 gcaatctctt tttggatctc aggtcgtttc cagtagccaa cgacgatttt attttgttta
    27301 tttaatcgag cattgatgaa cccatattcg cggtcaccat gagcggattg atttaaattc
    27361 atgaaatcca tatcgatcgt tttccaagga atgctttcgt tatattgcgt agctaaatga
    27421 agcaatggtt tttgtagaag ctttgtcccg cggatccaca tttttgctgg tgagaaagta
    27481 tgcatccaag tgatcacacc tgctacttct tctcgataat tgacctcttt catgattttt
    27541 gtgatcgtat cagcagtaac tgctaattct tgaaggcgga ttggataagg caattggcca
    27601 ttttcattca gttcatcgac catttcttgt gcatgtttct ttacttctcg cagtgcttct
    27661 tccccatata aatgctgtga tcctacaaca aaccaaaatt ctttttctcc aatattcaac
    27721 ataattagcc actcctttta tttgttttgt ccgtaatacg cattttttcc gtgttttcgt
    27781 aaataatgtt tatctaaaat ccgttgaggc agctcttctg aaaatgaatt gagctgtctg
    27841 gtaaataaat tcatcttgca tacttcatct agtacgactg cgttcattac cgcactttgc
    27901 gcatctttcc cccaagtaaa tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag
    27961 ggatcaattt tgcgttcttt gaatgtttct atgataactt ttcctgtttc atattcgtac
    28021 ccgctgtcga tttcctgttg tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta
    28081 tctgcatgcg tcgtccccat cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc
    28141 catgtcgagt gagtatggac gattcctccg atttgaggaa atgaacgata taaaacagca
    28201 tgagtaggcg tatccgaaga gggattgagt ttaccttcaa tgatatgatt gtccaagtcc
    28261 acaacgacca tgtcgtctgc tgtcatctct tcataactga ctccgctagg tttgataacg
    28321 aaatatcgtg tttcaggatc aaaagcgctc acatttcccc atgtatattt gacgagtccg
    28381 tgtttaggca agtcaagatt tgcttggtat acttcttctt tcagttgttc cagcattttc
    28441 tatcactcct ttttcttgca attgatgaat cgctgttttt tcaatgtcga tcccctcacg
    28501 gtaacgtctc ataaatatct gatagcctgc tatttcttct gctgaaggtg tgatggtgat
    28561 tccttggtct tgtccaaaca cgtcttctgc caagaagttt tctaaagact tcccatgtcg
    28621 atccatcata taggctgcta ataacgcgat tccccacgct ccaccttctc cggctgtgtc
    28681 catcactgta actggtgcct ccatagcaga tgctagaatc cgctgtccaa cttctggtgt
    28741 tttaaaaatc cccccatgcc ccactaaacg atcaatttgg acattctcag atttcaatat
    28801 atccattccg attctcattg caccaaaagc actggctaaa tggatccgca tgaaattcgc
    28861 taaatcaaaa cggctacctg gtgtacgtac aaataatggc cttccttcat ttactcctgt
    28921 aatgttttca ccggaaaaat aaccataaga aagtaatccc ccgcaatccg tatcgccttc
    28981 tagcgctttg ttgaataata ccgtgaataa tttatctctg cttatctcca tacccaaaga
    29041 attggtaaat tcttcaaaaa tactgaccca agcattgata tccgaagagc agttattcgt
    29101 atgaaccatg cctactagtt ctcctgaagg tgtagtgacc atgtcgattt ccggatggac
    29161 attttctagt tctttttcta atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt
    29221 ccctgtccgt ttggctacac tgttcgtagc gaccataccg gttcctgcat ccccttctgg
    29281 cggacaaacc gggattcctg gtctcaaatt tcccgtaggg tcgatcagat aagcccctga
    29341 ttcgcttaac ttgccagctt cttctcctgc tactagaact ttaggcaaaa tgtgatccaa
    29401 gtcaatcgct acgtgcgctt tttcgattaa ctgaccaaaa atatttttca tttcttgatg
    29461 gtaggttttc gttgaaggat cgataggaaa catgcctgat gcatctccta cgcctagtac
    29521 tttctcacca gtaagctgcc aatggacata acctgcaagc gttgtcaaaa aattaatttc
    29581 tttcacatgt tcttctttat tcaaaataga ttgatacaaa tgagcgatac tccatctctg
    29641 cggaatattg tattgaaatt tttccgttaa tattcgttct gcttcttcag tgattgtatt
    29701 tcgccaagta cgaaatggta ctagtaactg ttccttgcta tcaaatgcta gataaccgtg
    29761 catcattgca ctgaacccaa tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa
    29821 tacttctgct gccatttttc ggtaactgac gcgcagtcct ttccaaatat cttctaatga
    29881 ataggtccag ataccatttt ctagtttgtt ttcccactca taactaccag cggcaatagg
    29941 agtaaaagat gaatcaatca atacagcttt gatcctagtt gatccaaact caatccctaa
    30001 agatgtacga ccatctatga catctaacat actttgcgca tttacttcct tcataatgat
    30061 ttaactccct ccttctcgtc aaacctgatc aaaccgtctt tttagtagcg cttacaatga
    30121 ttattgtata cttttgtacg tacatgtcaa gcggttattt acaagaatac aataaaaaac
    30181 acttgttttg tacgtacata agttataaaa tgaaattgct tacaaaatct gatcaaccgc
    30241 cttctcgaaa ttctagaagg agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc
    30301 gtttatttat ttttttggcg catttggcgg cattctattt ggatatgata ttggtgttat
    30361 gaccggcgcc ttaccttttt tacagcatga ttggggtttg gcaggtaaag cttctttgat
    30421 aggatggatc acgtcttcgg tcatgttagg tgcgatcctt ggcggctctt tatctggtct
    30481 tctttctgat aagcttggac gacgaaaaat gattcttctt tctgcactta tttttatggc
    30541 tggttctgtc ctttctgcat ctgcccctca caatggttct tattttctga ttgcagcaag
    30601 gattcttcta ggtttagctg ttggcgctgc atctgcccta gttcctgctt atatgtctga
    30661 aatggctcct gctagactaa ggggaagatt gtcagggatc aatcaagtga tgattgcttc
    30721 tggcatgctc ctttcctatg ttgcagatta tttgctaaaa gtgttgcctg aaacgatggc
    30781 ttggcgcgtg atgttagggc tcgctgctgt ccccgcgctg attttatttt tcggtgtttt
    30841 agctctacca gagtcaccgc gttttcttat gcaatctggc cgcttggaag aggcaaaaag
    30901 agtattgaac tatattcgga caccaaaaga agctgaacag gaattcgaac aaatccagct
    30961 gaatgtcaaa caagaaaaga caaccggaac ttcttggcat actttgtttt tagaaaaata
    31021 tcgttcctta gtcattgccg gtattggtgt agcagtattc cagcaatttc aaggagctaa
    31081 tgctattttt tactatattc cgctgatcgt agaaaaagcg actgggcatg cagcgagcga
    31141 tgcattgatg tggccgatca tccaaggaat cattttagtt gctggttctt tgctcttttt
    31201 ggttattgca gacaagttca atcggcgaac tttattgaaa ataggcggta gcgtgatggg
    31261 actttcattc atcctaccag ctgttttagg aactgtactg gatgctcata caaattcgtt
    31321 gttgattctc ttgtttttat gtatttatgt cgcattttat tcttgcacat gggctccact
    31381 gacatgggta atcataggcg aaatattccc actagctgtc cgcgggcgag catctggcct
    31441 agcctcttct tttaattgga ttggttcttt cctagttggc ttgctttttc ctgtgatgac
    31501 tgcttctatg tctcaagaaa tcgttttcgg catttttggg atcatctgct ttttaggggt
    31561 tttatttatt caagagatcg taccagaaac tcgtgggaaa tctttagagg aaatcgaaca
    31621 aagtgcatct aaaaaaactt atcctaaacg tatttctatc catcattctt aaagaggttg
    31681 tgacagaagt ggtcagcttc aagaaataag aaggaaccgc ttagtttcca gtcacaagga
    31741 acaatttcaa aaaacagctt ctcatgtttg atgaatattg tgacttgtga ccagttggta
    31801 ggagaacacc gtttagtgga gaggtggtga aagaaacgtt cagccttgag cgatgacaaa
    31861 agctctcaca aacattgcga cgagtaaccg taggagcaat aagaggcggt aaaaacccgt
    31921 ctcttatttt tcgttttctt tcactgtttc ccgttcaact agtacaggtt catagagaat
    31981 atttggctgt gcctgtcctt tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc
    32041 caacttttct tttggatgcg tcaatgttgt caatttttta ttccctaaat ggcttaaaga
    32101 agaatcgtct gttcctacga tcgataggtc atctggaata tgatagccat tttctgtcaa
    32161 cttatctagc attaaactag ctatttgatc gttatagcaa atgatgccat ctaaagcttg
    32221 ttcttctttc aaccgttgta ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa
    32281 agtaatgata tcttccgtca aaaaacgcac attatactgt tcacacgcgc tgataaaacc
    32341 tttcatcctg tattttcctt gaagatcgtc gatttttgtg atgaacaaca gacgctgatg
    32401 tccttttttt atcaaatatt ctgctgccat aaaaccgact ttcacatcat ctaaacaaat
    32461 ataagaggca tttaactctt cataagcagc attgatcatc accatcggaa tgccttgttc
    32521 tctcaaagca acataaagtg ctaagtttgg gttatactga ttacttttcg taggctcaac
    32581 gatcaaacca tctaccccat aagcaatcat tttcttcaaa cactctttct ccaattcgtg
    32641 atcgttattc gtactggcta aaagcaatga atacccagat tctcgtaatt cattttcgat
    32701 tcctcggata atcgacggaa aaatataatc agagagatat gtggtgatga caccgatcgt
    32761 ttttgttctg ttcaatggtg ttttttcttt cttatagctg tcatctacat atgtgcccga
    32821 ccctttttct ttacgcagat agccctcatt taccaacaca gcgattgctt gccgaatcgt
    32881 atgtctgctg acgtcatata tttcttgcag cttgaattct gagggaatgg ttgtatttac
    32941 ttcgtattcc tcacgaagga tcttttgttt tatctcttct gcaatatctt gatattttgc
    33001 catttgcctt gccccttttt atttgtacgt acatatatca taacgagtct gcaaggttta
    33061 ttcaagcaaa aatcaaggtt tcttgtgctc ataaggcata cggaacaggt tcaccaaatt
    33121 ctggaaatcc ttgctcattc cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt
    33181 ctaacggatc ccctttttgg ttcttctctg gtcgagcatg atagatcaaa atatcaaagc
    33241 acccgtcttc tgaaaccgta aacgagttgt gaccaggacc aaattgctta tttttttcag
    33301 aagaaacgaa tactggctcc gataatttat gccatgattc ttttaataaa agattcttgt
    33361 gctcatcagc ccacagcagg cccatggcat agttttcatc tgttgcacta ccagaatacg
    33421 taatgaatat tttcccattg cgagtaagga ctgctggacc ttcatttact gaaaaacccc
    33481 gtttttccca ttcatactca ggtatagaaa ggaggatctg cttccccttt aacgtccaag
    33541 gattatccat ttcggataga taaagattag aattcccagt aatttcagga tcttgttggg
    33601 cccacacata atatagcttt ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt
    33661 cccttttcgt ttttatttgt cccttttcta tccaatctgt attcattgga tctatatgct
    33721 gattttcaag tacgaacatg cggtgatgat gagattcatc acgtatagtt ttatgattgc
    33781 ttgcagcaaa gtaaatatac cattttccat tgatataatg gacttcaggt gcccagatca
    33841 attcactcat cggtccttct tcatgagcct gccatacaat caggcttgca gcatgttcta
    33901 attcattcag tgactttgcc cttcttaatt caatcacttg gtagcctgga accgatcctg
    33961 taaaataata ataaccatct gtatgtttat atacccaagg atctgcccgt tgccgaacga
    34021 taggattatt ataaagtgtc ttcatcataa caaatttcct cacattttct ttattctaat
    34081 tacattccaa gagtattttt ctatccctac atttaattcg tcatgttttt ctattgaaaa
    34141 atagtttatt tcaggtttta ttttttctgg ttcttcacgt gtatttttgt ccaacagatc
    34201 gctactggtt aatgtgtagt gctctccttg atctagcaaa ttgaaatcat gttcaatcgt
    34261 tatcttttgt tgttcatttg tgcgattgat caagaaaata gtcagctgat tgccgttatc
    34321 tacgatcaca ctgtccacat aaggaacatt ttttatcttt tcacaatcat aaacagggca
    34381 aatgatttgt tgtttcaata cttgaccttt agcatatttc gataagtgtt gcatcacata
    34441 ataagttgga ttcctccatg cttgtccttt agcatctgtc agtattaaag gtattgtgtt
    34501 tactaacaat gattgacaac taatctttac gcgatctgcg tgtcgtaaaa tagccagccc
    34561 taatgaacca aataataatg tatcttccat agtaaagtaa caccaatcga tgggactacc
    34621 gacttcaaaa tccctatgtc ttttttccgg ttgggcatgt acattccatt catcaaacgc
    34681 tagatacatt gttttctcac ttctcaaata tccttttaca taatcacaag tagcaacgat
    34741 ttcttcaatt tgacgatcga atcgataagc ttttgcaagg tatgtcgctg tatcatcacg
    34801 ctcattttta ggaggtttag tcaagtcttc ttcatcatac ttatcaatat agttatgcaa
    34861 agctaaataa tctacattct cataagcttc ttctaatgcg atacgatccc attcagggta
    34921 cgatggtaaa cgtggcgtag agcttccaac taagattgtt tcaattgaat catccactaa
    34981 cttcatgact tttgaggctt catttgctaa cctaccatat tcataagcac tcttagcccc
    35041 cacttgccat ggaccgtcta gctcattccc taaacaccat aatttaacgc caaaaggctt
    35101 tttttgccca ttttttattc ttaaatcact ccagaaggtt ccttttggaa aattacaata
    35161 ttctactaaa ttttttgctt catcaattcc ttttgttcct aaattcaccg tgaatatcgg
    35221 tttcgcttgt acatgactga tccatttaaa aaattcgtgg agtccaaaag aatttggttc
    35281 tatacctcgc catgctaaat caattttttt tggtcgttgt tctttcctac ctacagtatc
    35341 cacccaatca taaccagatg taaagtttcc tccaggatag cgaatgacac ctaattctaa
    35401 ttcctttaca taagagagga catcttgtct aaacccgtca ccatcagctg tttcatggcc
    35461 cggttcataa atccctgtat agacgacact tcccatatgc tcaacaaatg agccaaacag
    35521 ccgagaactg atctctccta ccgtaaaatc ctctctgatt tttactttca cttcttcgtc
    35581 caacagcttt ccctaccttt catttcctat agtcaagata atagatacaa gtttccatac
    35641 gccaccagca aagataaaaa taaaaggagg gaaaagaaat atgagcagta caaatcctaa
    35701 taacaatacg caacaaagaa tatttctcaa aaaatcaaaa aatactttcc atgcactttg
    35761 ttcaaaaatt tgtaaaaatg tgatatcttt gttttcagaa atcacatata tccaggataa
    35821 aagaaacatg gaataaataa gtaaaactac atatccacta attgaaagta cgattgtctg
    35881 ttcttctaat aattgattaa caagaaaata aataatgatc gttaacagac taaggaacaa
    35941 gttcttacca aaatgttgct ttattccttg aaagaaaaaa taaatgatat ttcctgatcc
    36001 ctcacctttc cattgcgcca ttacataaaa aacagcacat gtagcaggca cgatagtaat
    36061 aacaggcaaa cacacgatta cccagagaat acctaataag atgtaatcag aaaccacaat
    36121 caactgtcca taaaatttag tctcaactat tttcatcttc ctcactcttt cattccagac
    36181 atagctgttc cttgaacaaa atatttctgt gcaaaaatat aaaccactag taatggcagc
    36241 attgaaataa ccgttccagc cattacaggt ccataaaaag aaatgttctg ccctgtaaat
    36301 aaagctaacc ctgctggtaa agttctcatt tttgaactac ttgtcaatat caatggataa
    36361 agcaagtcat tccaactatt catcagcgtg aaaatcccta atgataaaag agtaggtttg
    36421 ctcaatggca acatgatttt caaaaaaata ttaaactcac ttaatccatc aatgcgtgct
    36481 gcctcttcca aagaatctgg taaagtaata aaaaaggaac gcatcatgaa gataccaaat
    36541 gcagttgtca tacgaggaat gatcaaacct gcataagtat caagcatgcc aagcaaattg
    36601 acttcaataa atagaggaat catgaagact tggaatggga tcatcattgt cagcaaaacg
    36661 aaatagaaca aaactgattt tcctttgaaa cgcatacgag caaatgcata ccccgcaaga
    36721 gaatcaaaga atacagaagt gatcactgtc cctccagcaa aaatcactgt atttttgatg
    36781 taatctaata aagggatcgt tgtccaaacc ttaatatagt tatctagggt atagctatct
    36841 gcaaatattc taggcggaaa tgagataatg tctttttcgt gtttaaaaga agaaataatc
    36901 agccacaaaa aaggaaacgc aataaccaac aatataacta gcatactaat cgttcgaatg
    36961 atttttttac cactaactgc ttttcgacta ttcacagtaa ctgtaatcga gctttctggt
    37021 gccatacagg tttccctcct atttcatttg tttttctttc cgtgacatgt acacattagt
    37081 ggagatagct atagttgcaa caatgataaa caaaaccgaa gacagtgctg atgcatagcc
    37141 caattcataa ggtgctgtaa atcctcgact gtaaatatat tgaacagctg tttctgtctt
    37201 aaattgtggg ccaccagcgg ttgcaacata tacttggtca aacacttgca tcgaaccaat
    37261 cagattcgtt aaaatacaaa aacctaagga gggaacgatc tgaggtagcg taatgttaaa
    37321 aaactgctgt cgatttgttg caccatccat ttgagcagat tcatacaaag agggagagat
    37381 tccctgaata gcagtcaaga gaatcgtcat agtaacacca aaattcttcc aaacagtcat
    37441 gatggctacc gtcggcatag caagggttgg atcgcgaaaa aattgaggga tcggcaagcc
    37501 gacttgtgaa aacaagtaag gaatcgcccc tacattcgga tcaagcaaca tagaaaaaat
    37561 aatcgcaatt gacgtcaatg aacaaataac tggaatgtaa aatgtcgaac gacaaaaacg
    37621 attgaatctg gtatttttag ataatgctac tgctgctaat aaaccaacga taatttgtgt
    37681 tggcgtttcg agtacggtga aatataaaga atgaatcaac gaattaactg ctcgttcatc
    37741 actaaatata cgtgtgaaat tttcaaaact tacaaaattt cttgatgtaa agaaaatatt
    37801 caaatctgta aaactaatag caaatgttcc taaaagcggt atgacagtaa ataataataa
    37861 aactaaaaaa gatggagtta aaaacaaata cgcagcacga tcaggacgat tccaatattt
    37921 ggtgaatccg ttcatatagt ccacctcatc tttcattttg ataaaatata caatcttggt
    37981 ttaaacgaat ctgaataaga tatccgaaca atcgctgtga tttttgcaat aacacctatc
    38041 tgcaaatact aggtcgatca ttcagatatc tgtatcttgc caacagtcta tcctgtataa
    38101 agttctgtac tatttttgca gtaattgatt gatggcatca tctgcatcgt tcatgagtgt
    38161 ctcaggatct tctcctgcta ataatttttc aatcagtgga ttgatgatat cgttattgat
    38221 cgcagagatt tgggtaaaac ctggtaaata tggctctgca tagtccattt gtttcgataa
    38281 ttcagataca ataggatcat tttttacttc ttcatctttt gctactgaat ccaagtaggc
    38341 aggaaaacca ttttcgatac tccatttttt accaatttca gttgtattcc aatatttcaa
    38401 aaactcatag acagcttctt ttttattatt atctgtactt gccggaatgg catatcctac
    38461 gccatctaaa atagctgttt ttttgtctga atcactccct tgtggtaagg tagtcacatt
    38521 ataatcaatc tcgtttgctt tcaatccgtt atttaaccat ggcccattga tttcaatggc
    38581 tagttgacca gcattcatta aattgtccat ttcggcgcca gaaatcgatt ctggaccagt
    38641 cttgtctaca tggatcattt tttggatcgc ttttaacgtt ttaaggttct caggagacgc
    38701 aaattcagac ttagtataat catcgttcac taatttaccg ccatttgcta acatccagtt
    38761 atataacggt gcagttccat ccttattgaa gacaaaacct gatacatttt tactactatc
    38821 agttaatttt ggtgccattt ctgtcaattc ttcccaagta gtaggtggtt gttcagtatc
    38881 taatccagct tgtttaaaca aatcattatt ccaatacata tacatgcctt gtacctgcat
    38941 ggggacaaaa tactgtttac cttctacttc acctaattca atagccgttt ctttaaaatc
    39001 agatttatct actccatcat attcccagaa atcgtctaat ggttgtaccg ctccgttttc
    39061 tacatactgt gcaaaatctg gataatttaa tgcaataaag tcaggtgctt ttttagaaga
    39121 aatcgctgta ggaagttttt cattcaaatt ggcccaagtc atgatatcca tttcaatttt
    39181 gatgttttta tcatttgttt tattaaattc attaacgatt tcttgcaata tttcaccatc
    39241 agatgcagta aacccattcc aaaaagtcaa agtcacttct ttatcagaat cagattctgc
    39301 tgaacttccg ccaccgcaag ctcctaaaat acctgttgca gctaccaaca aagagccaat
    39361 taaccatttt ttacctttca ttttttgttc ctccttcatc tgataaacga catctgacca
    39421 cattccaaga tagcggtttc aataaacaat tgattttccc atcttcagct tttacatttg
    39481 ttgaatgact tggttttact tgttcacttt taggaagatt agttgctttt gtatcaaatc
    39541 cactcatttc agaaaaatca atcactccct ctaatgataa ttcagacaag tgaacagtta
    39601 gattcatttc ttcttctcca cggttaactg caaatatgac aagttctttg tcatttatta
    39661 cggctatact ttctaaatac ggcacttctt caaaatcttc cgtcgcatag tttgctgaac
    39721 gaatttgtgg gcttaacacc tctccttgtc catacacagc agcttgcata aatggataat
    39781 agatcgtctg tctccaagct tctccgtttg tttctgtcct gattggtgca ataacgttta
    39841 ccaattgagc aagacaagca atctttactc tatctgcatg tttcaataaa gtgattaata
    39901 gacagcctaa tagtaatgca tcttcaaaat tatagttatc ttctagtaaa ggaggagcaa
    39961 tctgccaagg cgctgcttgc tcatcttgtt ccgctgaatg ataccaaata ttccattcat
    40021 caaaggacag gttgatctgc ttcttactat gtttcttcgc cttgactgta tcgcacatcg
    40081 ctgtcacttc agaaatgaac cgatccatgt ctaaggaacg tgcaagataa tgagaaagat
    40141 catttttttg gttgccataa tattgatgca gagacaaata atctatttga tcgtacgctt
    40201 gatcaagaac tgttaactcc caatcaccaa atgtaggcat ttgccgattc gaacttccgc
    40261 ataaaaccac ctcaattgaa tcatctacga gtttcatcgc cttagccgtt tcctctgcta
    40321 accgtccata ttcttccgct gttttatgtc caatctgcca aggaccgtcc atttcattcc
    40381 ctaaacacca tgtcttaatt ccaaaagggc tctcttgacc attcgaacgc cttaaatcac
    40441 tccaataagt tcctttagga aaattacaat attcgactaa attacgagct gcatctactc
    40501 cacgagtacc cagattgaca gccatattga tttcggcccc cacatgctga caccagttat
    40561 aaaattcatg aatcccaact tcattcgttt caattgtccg ccaagctaaa tccaaccttc
    40621 taggtctatt tgctatgggc ccaatactgt cttcccaacg aaatcctgac acaaaatttc
    40681 caccaggata tctaatcatg ggaatgttca attcttttac caagtgaatg acgtcttggc
    40741 gaaatccact tttattagct gatggatgat ctggctgata aatcccttca tatactgctc
    40801 gtcccatatg ttcaataaat gaactgtaca accgcggatc aactttgcct acacgcttga
    40861 cactactaat caacattgtg gcttccataa ctgacctcct ttaatttata catgaataat
    40921 acacctcgtt gaaagcgttg tcaataatat aaatgttaaa cttacttatt ttttatcgat
    40981 tttaattaac caaaatatta ataatgatta aaacaaataa tttaatataa cttttattaa
    41041 caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca actacaaata
    41101 aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat caagatcatc
    41161 cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact tggagtgagc
    41221 agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat caaaacagaa
    41281 aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat tagttttcca
    41341 gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg tcactataca
    41401 aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat cgggaaagtg
    41461 gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct ctggtttacc
    41521 agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac attggagatg
    41581 ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa ttggccttca
    41641 gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc tggagatttt
    41701 gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa tcaatacggc
    41761 ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga acccttgtca
    41821 aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg aatcgaagtg
    41881 aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt cggtaattac
    41941 gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct attttataaa
    42001 acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt atttccagaa
    42061 atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc accatgcttc
    42121 gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat caatttcatt
    42181 atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt caagatctgc
    42241 agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca attgtttccc
    42301 ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac caaatttcgt
    42361 gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc ctgctctttc
    42421 tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc ctttgttaaa
    42481 atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa cagctggttt
    42541 gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt ttgcatcttc
    42601 ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt tttcatcttt
    42661 tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta aagtagaatc
    42721 ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt tcaaatatgc
    42781 tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg gtttgacacc
    42841 taattcattg ataatttctg tctgcaggct catttattct cctcctatgc ttctgtatga
    42901 agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt ccagcattca
    42961 gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc ttcccatagt
    43021 gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa ttcgtaaact
    43081 ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc tcgaacagtt
    43141 ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc tcgagggtct
    43201 tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg agtgatgcgc
    43261 cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa cttgatcgta
    43321 tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc accaagggct
    43381 ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat tttagctttt
    43441 tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata gatttttgct
    43501 tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata ggccatatct
    43561 ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt cgccacgcga
    43621 atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa cacgcagtct
    43681 ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga ttggactaac
    43741 gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg tacattgctt
    43801 gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc atccagttct
    43861 tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga tttgatacga
    43921 gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc tgtttctact
    43981 aattggcagt gcgctaaagg cccttccact tggatcaatg gttcatttgc aaaaactagt
    44041 tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa atactccaaa
    44101 aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc attgaacgtc
    44161 agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta tccgtggtta
    44221 aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg acctaattcc
    44281 cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa actgtcgtct
    44341 gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc ctacccatta
    44401 taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag cacatgtttt
    44461 acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc ttattggtat
    44521 ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac gaagaaatta
    44581 gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca atcgtagccg
    44641 ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa tcaatggcaa
    44701 aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa agaaacggat
    44761 ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat ataccttgcg
    44821 ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag taagaaagca
    44881 gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc ttgtttttta
    44941 ttgtatacat tagatatgga gcaggttacc ataaagatga ttattagtcg ttattaaata
    45001 gaatccttcg tatttttttg ttctatcact acaatatctt tatttggacg tttgaattgt
    45061 attccggcac gttctagctc ctgtttcact cctacatgga ctttgacatc ttctcgtgtc
    45121 atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt tttcttcgta
    45181 ttgatcatca cgacttgctt tttatcatat gtgtctacca atagcagttg tgttcgatct
    45241 aacgaaaata atttgtaata attaatagcc gtattcccaa aaaagaattc cactacttta
    45301 ggataaaatg tcttaatatt taaattacta ttcacaggtg tttcgattag cttcatctca
    45361 ttcctctttt ctatatagtc gatttagatc caatcttaag ttatgttacc atctttccta
    45421 gcattaagga atcggttaca attgaacctt taaagacaaa aggatttacc ttttatcact
    45481 taatagtata acataatttc atgagaaaaa aatacaattt tttcttgacc atctcgttac
    45541 gtaaaggatt atgcttacat ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa
    45601 tgtcagagat atcaggagtc agtccgagaa ccttgcgctt ttatgatgag attggactgc
    45661 taaaaccagc taggatcaat tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc
    45721 gtctgcaaca catcttattt tatcgaacaa tggcattcaa gttggatgat atccaagagg
    45781 tcttagacaa tccatctttt gaccaccaga aagctttaat caagcatcag caaatgttgc
    45841 tggaaaaacg ggcacaaatc gataccctct tgacaactgt ccaacagaca cttgatatgt
    45901 acgaaggagg aagaaaaatg agtgacaaag aaaaatttga agggtttaaa caacaaaagt
    45961 tgaaagaaaa tgaagaatcc tatggacaag agattcgaca aaaatacgga aaaaaaaagg
    46021 ttacagcatc caatgaaaaa tggatgaata tggataaaga aacgtatgaa gaaatgcaaa
    46081 ctgttgaaaa gcagatgtta tttgatctca ctctttactt aaaagaacca gaagatgaaa
    46141 gcttagcaga ccgtatcttc caagctcata agaagtggtt atcttacagc tggcctgaat
    46201 atcagccaaa agcgcataaa ggactaggaa tgatgtacga agccgatgaa cggttcactg
    46261 catattatga cgagcgaagt ggtcatggtg cagcaaaagc tttaaatgaa attatccaaa
    46321 aaaaggcgta atcttttaca tgatccaaca aaaaagtaca cgtaaaatga cctaataaca
    46381 aaacagcaga cacccaagtt agatttttat gctaactttt ggtgtctgct ccattctttc
    46441 acaagttttt tcatcctact ttaaatatat tcatcgttga ataaacgtat aaggtatttt
    46501 cactttatcc tttttttctg taagaaacaa ttgaaatgcc atttctccgc aagcttccag
    46561 atggtggtcg atggttggga aaccaagaag ctcgctgatc agcaaattat cgcgtccaat
    46621 caccactggt gtcttatctt gcagatagct ttgcaagatg cctgctgcaa ccatatccga
    46681 atttgtaaag acaccatcaa ctccaagctc tttaaaaaag aagcctgctt gttttccttt
    46741 ctctgcatct atacaatccc aaaaaacaag tgattcatca aaagatggaa aaagttgatg
    46801 acatagctgc aaagtgattt ttgagttata acttagcctg ccacttcgcc ccaacgtgat
    46861 gcccaattgc tttatacctt gctgcttcaa ataagtaaga ccttctgtaa ttgattgttc
    46921 tcgatcaata aagacacagc ttgcctgttt tcccttgatt tcttcacaaa aaactagagg
    46981 tccatatttt tgatatgcca ataaatcatc tagtgtattg gcacgagacg taatgatcaa
    47041 tccatcgaaa gctttagctg caaattcttc aagatacctt cgctcttttt ctttctgata
    47101 gcccgtcggt aaaagtgtca ctttgtaatc ataatcaaac gctgcttcaa tgatcccact
    47161 gatcaactga tcaaaatacg gatgattggt ataaggcaga atcactccaa tgcttttcgt
    47221 ttttccataa cttaactgtc tggcatttgc attagggata taatcgactt ctttcatgac
    47281 cgcaagtatt tctttccttt tttcctcatc tacatatgga tgtccattga ttacacgtga
    47341 aatagtagaa actgaataac ctgtgagctt tgcgatatct cgaatgtttg ccactgctgc
    47401 ttcgccacct atacaaaaag attttcttgt aaatagtcta ccacagctgg accgcattca
    47461 aaaaactgat acatttttcc ttcatgcgaa aaactgatcc gctgcccgcc ccaatatata
    47521 tcttttgtca aattttcgat ttcattcatt ggtatttctt ttactatatt cctattcaac
    47581 ccttgaaaac tgtacaacgt taatacggat cccttgaact cgacaaacgc ataaaaataa
    47641 ttgttcgttg tgccatctgt tatctctaca taagtattca ttgcaagccc tccttttgat
    47701 actttgagta tagtgtatga aacgcgtctc atagcaagga gtttgttttg ccaaagaaaa
    47761 gttttattcc tctttcatta ttaccaaagt ctcttatttt aaattgaata cattataatg
    47821 atactgtaaa tatatcttta ttattagtga atggatattt acttttaata gataaaatat
    47881 tataactatt aaaaataact attttcatta taattacaca caaaaaagag cgtgagacaa
    47941 aagtaaagaa ca
//
LOCUS       DACDSY010000005        43631 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDSY010000005 DACDSY010000000
VERSION     DACDSY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.005"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(271..2652)
                     /locus_tag="IWK44_000284"
     CDS             complement(271..2652)
                     /locus_tag="IWK44_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9548842.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2642..3289)
                     /gene="recU"
                     /locus_tag="IWK44_000285"
     CDS             complement(2642..3289)
                     /gene="recU"
                     /locus_tag="IWK44_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP9548843.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3353..3892
                     /locus_tag="IWK44_000286"
     CDS             3353..3892
                     /locus_tag="IWK44_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP9548844.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3982..4401
                     /gene="gpsB"
                     /locus_tag="IWK44_000287"
     CDS             3982..4401
                     /gene="gpsB"
                     /locus_tag="IWK44_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP9548845.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4423..4790
                     /gene="rnpB"
                     /locus_tag="IWK44_000288"
     ncRNA           4423..4790
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWK44_000288"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4999..6165
                     /locus_tag="IWK44_000289"
     CDS             4999..6165
                     /locus_tag="IWK44_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP9548846.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6211..7707
                     /locus_tag="IWK44_000290"
     CDS             6211..7707
                     /locus_tag="IWK44_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP9548847.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7788..8471
                     /locus_tag="IWK44_000291"
     CDS             7788..8471
                     /locus_tag="IWK44_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9548848.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9116..10306
                     /locus_tag="IWK44_000292"
     CDS             9116..10306
                     /locus_tag="IWK44_000292"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP9548849.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10408..11361
                     /locus_tag="IWK44_000293"
     CDS             10408..11361
                     /locus_tag="IWK44_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP9548850.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11430..12779
                     /locus_tag="IWK44_000294"
     CDS             11430..12779
                     /locus_tag="IWK44_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9548851.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12962..13285)
                     /locus_tag="IWK44_000295"
     CDS             complement(12962..13285)
                     /locus_tag="IWK44_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548852.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13414..14577
                     /locus_tag="IWK44_000296"
     CDS             13414..14577
                     /locus_tag="IWK44_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548853.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14647..15996
                     /locus_tag="IWK44_000297"
     CDS             14647..15996
                     /locus_tag="IWK44_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9548854.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16041..16472)
                     /locus_tag="IWK44_000298"
     CDS             complement(16041..16472)
                     /locus_tag="IWK44_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9548855.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16860..19502
                     /gene="alaS"
                     /locus_tag="IWK44_000299"
     CDS             16860..19502
                     /gene="alaS"
                     /locus_tag="IWK44_000299"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP9548856.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19702..20298
                     /locus_tag="IWK44_000300"
     CDS             19702..20298
                     /locus_tag="IWK44_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9548857.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20420..21568
                     /locus_tag="IWK44_000301"
     CDS             20420..21568
                     /locus_tag="IWK44_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9548858.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21606..22019)
                     /locus_tag="IWK44_000302"
     CDS             complement(21606..22019)
                     /locus_tag="IWK44_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP9548859.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22098..22529
                     /locus_tag="IWK44_000303"
     CDS             22098..22529
                     /locus_tag="IWK44_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP9548860.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22628..22861)
                     /locus_tag="IWK44_000304"
     CDS             complement(22628..22861)
                     /locus_tag="IWK44_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP9548861.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23044..24714
                     /locus_tag="IWK44_000305"
     CDS             23044..24714
                     /locus_tag="IWK44_000305"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP9548862.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24861..25247
                     /locus_tag="IWK44_000306"
     CDS             24861..25247
                     /locus_tag="IWK44_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548863.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25234..25932
                     /locus_tag="IWK44_000307"
     CDS             25234..25932
                     /locus_tag="IWK44_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9548864.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25937..26401
                     /locus_tag="IWK44_000308"
     CDS             25937..26401
                     /locus_tag="IWK44_000308"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP9548865.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26398..27309
                     /locus_tag="IWK44_000309"
     CDS             26398..27309
                     /locus_tag="IWK44_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9548866.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27544..28083
                     /gene="pyrR"
                     /locus_tag="IWK44_000310"
     CDS             27544..28083
                     /gene="pyrR"
                     /locus_tag="IWK44_000310"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP9548867.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28097..29380
                     /locus_tag="IWK44_000311"
     CDS             28097..29380
                     /locus_tag="IWK44_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP9548868.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29393..30319
                     /locus_tag="IWK44_000312"
     CDS             29393..30319
                     /locus_tag="IWK44_000312"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP9548869.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30338..31618
                     /locus_tag="IWK44_000313"
     CDS             30338..31618
                     /locus_tag="IWK44_000313"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP9548870.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31623..32699
                     /gene="carA"
                     /locus_tag="IWK44_000314"
     CDS             31623..32699
                     /gene="carA"
                     /locus_tag="IWK44_000314"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP9548871.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32704..35889
                     /gene="carB"
                     /locus_tag="IWK44_000315"
     CDS             32704..35889
                     /gene="carB"
                     /locus_tag="IWK44_000315"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP9548872.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35943..36719
                     /locus_tag="IWK44_000316"
     CDS             35943..36719
                     /locus_tag="IWK44_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP9548873.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36716..37648
                     /locus_tag="IWK44_000317"
     CDS             36716..37648
                     /locus_tag="IWK44_000317"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP9548874.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37645..38361
                     /gene="pyrF"
                     /locus_tag="IWK44_000318"
     CDS             37645..38361
                     /gene="pyrF"
                     /locus_tag="IWK44_000318"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP9548875.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38368..39057
                     /locus_tag="IWK44_000319"
     CDS             38368..39057
                     /locus_tag="IWK44_000319"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP9548876.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            39030..39299
                     /locus_tag="IWK44_000320"
     CDS             39030..39299
                     /locus_tag="IWK44_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548877.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39337..39978
                     /locus_tag="IWK44_000321"
     CDS             39337..39978
                     /locus_tag="IWK44_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9548878.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40009..40713
                     /locus_tag="IWK44_000322"
     CDS             40009..40713
                     /locus_tag="IWK44_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP9548879.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            40710..41612
                     /locus_tag="IWK44_000323"
     CDS             40710..41612
                     /locus_tag="IWK44_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9548880.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41770..43476)
                     /locus_tag="IWK44_000324"
     CDS             complement(41770..43476)
                     /locus_tag="IWK44_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP9548881.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13975 a         7691 c         9486 g        12479 t
ORIGIN      
        1 caatgggatt attaagaagt ggcactaaat aaaagactag tgtgtattta gccgatttaa
       61 ttataaacaa gtaaatggat aaagtatatg taatgataaa agaaaaatgg attttctaat
      121 ttttaagcta aatgttagat cacacatgta tatgctggat agtcttataa aaaatcatgt
      181 aatacagctt ggtacatgat ttttatttgt agctgttttt tacatagaca caaaaaagaa
      241 accacgcttg tagacgtggt ttctttttta ctatctgttt ccagaagctg aggtgctgct
      301 ggatggtgta tggtcatttg cagcatccgc tccgctagtt gctggctcac tggaagaagc
      361 aggaggagca gatgaagacg gtgtcgagct ttcttcggat gaagaagaag gaaccgttgt
      421 tgaagacgct ggctcgcttg tttctgctgt cgtgctttct tttgaggact cagcagttga
      481 ttcgctttga ctagtagagc tagagctttg agtcgttgtc tctgttgttg agcttccagg
      541 agtctggaca tagctggaag atggaatagt tgttgatggt gtaattgcat tcgatctagc
      601 tgtatattga tctttgtagt acagttcacc gcccacacga attaatccgc ttggcatctc
      661 ccagtctgta tttgtcacgt tagctgaaac atactgcata agctcgcgat aaacatcaga
      721 ggctacgtgt gacgattctg aagtgacagg tgtcattttt ttattgtaac ctgtccacac
      781 ggaaatcgaa taatttggcg tatagcccgc aaataggata tctggataca ccccagaaga
      841 aataccaagt tttgcatact cgtcatctgt gtaattggat gttccggtct taccggcttg
      901 atataaccca gcgatttgcg cattcgtacc agttccttca gtgatcgtat cttttaaaat
      961 gtctgtgatc atataagctg tttctggaga catggctgtt tttccatctg gctcataagt
     1021 ttcttctgtt ccgtcttgga agacgatttt attaacatat tgaggtttgt aataagtccc
     1081 tccattggcg aaagccgcat aggctgctgc catttttaag gaagaagctc cgtattttgt
     1141 cccatcttgt tcttccgtat tactagagat cgcattcgat tgatggatcg tactgtattc
     1201 gatcccaaga ttttttaaga aagaagccac tttatctgaa ccgacttcat tgaataattt
     1261 gactgctgga acattacggg ataagtaaag tgcttggcgt aaagtaatgg tgcccatata
     1321 ttgattatcc cagtttccta caggagtcga agtcccttca tagttatacg gtgcgtcagt
     1381 aatagttttt ccagtagaat atttcaaata ttcaaaagct ggtccgtaat ctgtgacagg
     1441 tttcatcgta gaaccaaaat cacgggaagt attcactgct aagttgttgc ctaaagttac
     1501 gtcttctgcg atgtgacgtc cgccaatctg ggcttttact ttcccggtat tggtgtcgat
     1561 aagggtggag gcaacttgca tctcgtcgtc tgggtaagag acatactgat ccgtgttgac
     1621 gatatcatat agtttctttt gggcgtccag atccaaattc gtataaatct ctaaaccgtc
     1681 tgtgtagaca ttcttatctg ttttttcttg tacttcattg atgacttctt tgacataatt
     1741 atcgacgatc ttagtattgt catcggattg agttagttct tgcagaccat ccgttaccgg
     1801 tacattaaca gcttgatcat attctgtttg agagattttt tcgttttgaa gcatcgtata
     1861 caaaacagta tctcgtcttt tcttcgcctg atctggataa acataaggat cataggcgga
     1921 aggggcttga ggcatcccag caagcaatgc tgtttgcggt aaagaaagtt ctgataattt
     1981 cttaccaaag tacatctcag aagctgtctc catcccgtac aagccgtttg acatatacac
     2041 tttatttacg tagtaggtca agatctcttg tttcgatttt ttttgttcca atcttacagc
     2101 catccacgct tcttgggcct tgcgtttcag agtctgatcc tcggcactgg tggagaagaa
     2161 ggaaagtttg atcaattgct gtgtcaaagt acttccgcct tgcagtccgc cagaggtaaa
     2221 gttagagagg gcagaaccga tgatccgaat agggtcaacc ccgatgtgtt tataaaaacg
     2281 gcgatcttct accgacacga tcgcatcttc taatgttttc ggtaattcat tcgcagaaat
     2341 tttttcacgc ttttctgcgc ctaaatcttc gaatagttcg ccatcttgtg tatatagttt
     2401 ggaagatacg gtagcgtcta attttttatc cgtcaattcg ggcgcatctt ttgcgtaata
     2461 ccagaaaagt cctacacctg ctaaaaagag gatacagaaa aatgagagta ttcctaaaag
     2521 gatcttgata aataatcctt ttttatgtgt tccagaggac tttccagaac ctttgccaga
     2581 attcttcttc acactctttt taggagtagg ctgtttctgt cgtctagagc ttctagtttg
     2641 ttcatttgcc attcttgctt tctccttcaa tagataatgt tgataaatag aagtcaaccg
     2701 cgtccaaata aggaatccgc ggagaaatac cagttttgat ctctattcct agttcttcga
     2761 tcaaagataa aggtagtgat ttgcgtcctt tttccttttg ggcgtcccag taagcaatca
     2821 aatgaacact attcaaataa aaacagcgct tcaaagacga aaaccataac aagacaaagc
     2881 agaccccatt ttgttcgagg catgctttca tatgttcgat ttgatgggca tggaagtttt
     2941 taaagggaaa agatgttttg ttcttggttt cttttgcttc aaagtccaag taaaagcctc
     3001 gatagacgcc attatagtct gtagttgagg cttgggtaaa gtaggcttcc ttgatcacag
     3061 cagcacttct cttaggatag tctactttca cgatttggat aggggtaggc tttttatgga
     3121 taaccgccaa cccgcgggaa agataatatt tattactttc attgattgct tcttcaaagc
     3181 gcatcccccg attgccgaat tcagtgatct gatttctttt ttcttgaatt ttttcgacgg
     3241 gagaggcatt cttataataa ggttggccat ttggatagcg caataccatc tttaatcaca
     3301 ctcctaggcc acattatagc aaaaaagcgg atagaaagaa aaggaatctg agatggataa
     3361 gtcaaaagta atctatgtgt ctgggtatcg aagtttcgaa ctcggtgttt ttaaagaaaa
     3421 tgatcctaaa atccaagttg ttaaaaatgt tttaaaaaaa gaactccatc agttagcaga
     3481 agaagggctg gaatgggtac ttgtcagtgg gaatttgggc gtcgaactat gggctgtgca
     3541 aatagtagcg gaattgaaag aagaatatcc ggaactatct cttggtattt tgtatccttt
     3601 ttctgatttt ggtaataact ggaatgaagg gaaccaagaa aaaaaacaat tagcagaaca
     3661 attagcagat tatgtcgaag ctgttagcca tcaaccctac caatcaccta gtcagctaaa
     3721 gaatcacact cgttttatac ttgagcatac agtgggctgt ctgctgatct atgatgaaga
     3781 attccctgga aaaacgaagt atttttttga agatgcacag aattaccaag cagatcatcc
     3841 ctacgaaatt cggctgatca gtatggacga tcttcaaaat tttagtgaat aatcgatatt
     3901 gtgtttgatt tatcaacttt tttcctatac aatgaaaaag gtagaaatgt tattacgtaa
     3961 atgaaaacta gaggtgtaaa tatggcgaat ttggtttata gtcctaaaga cattttacaa
     4021 caagaattca aaacaaaaat gcgcggctat gatcccgttg aagttgatga attcttagac
     4081 aatatcatca aagattatga agcttacagc aaagaactcc ttgctttaca agaagaaaac
     4141 gatcgattga gtgcaaaagt agctcaatta tctaaaactc aaggagcagc tcaaacacgc
     4201 gtgcaacaaa cagaggcacc aaagagcgca gcagtgacca attttgatat cttaaaacgc
     4261 ttgtcaaatc ttgaacgtga agtatttggt aaaaagcttg atcaacaagc aagtgcagtc
     4321 aaaccagcac aacctaatcc aaataactac acaaatgcag acacaagctt agatgataat
     4381 gaaaaaacac gtcaatttta atttttgatt aatgaatttg cggttttcgg gtaatcgcgg
     4441 tctgacttga tcaggctgag gaaagtccat gctcgcacag gctgtgatgc ctgtagtgtt
     4501 cgtgcttagc gaaaccataa gctaaggtac tctttggagt aacggcagga aaacaagcta
     4561 aggcttcggc tatgcttaag tatccttgaa agtgccacag tgacgaagcg gctggggaaa
     4621 cccatccggt ggaacgcggt aaacccctcg agcgagcaac ccaaacaatg gtaggggcgc
     4681 tttttgtctg gaagaagaac aggacaaaaa ggcagtataa ctgcagatag atggttaccg
     4741 ctttgttttt tctccctgga aaagcaaagt acagaacatg gcttatagaa aaccgcaaac
     4801 atcagggaat taggtcgtga caaaagtaat gcgttataag aaacaagaaa aacatttgaa
     4861 agctattttt caatacttca gatgaatcgt tcttattaca aaacaaactt ttgtatcacc
     4921 ggttattcgg aatttgagta aaagtgtggg gtaggactga tgtcccacct ccttttttta
     4981 taaataagga gattgtgtat ggaaaaaaac aggaagttca acctcgtcgc aactgcggca
     5041 agcgggctag aagcactggt tggtaaagaa ttaagagatc ttggaatcga atgccaagtt
     5101 gagaacggac gtgcacgttt tgaaggaaca atggaaacga tcgctacagc taatttatgg
     5161 ttacgtacag cagatcgagt aaaaatcgtg gttggggaat tcgatgcgta tacgtttgat
     5221 gaattgtttg aaaacgtaaa agcattgcct tgggaagatt ttcttccact agatgcagct
     5281 tttcctgtag ctggaaaatc gatcaagtcg aaactatata gcacacctga ttgccaagcg
     5341 atcacaaaaa aagcaatcgt tgaacgtctc cgtacctatt atcaccgtcc agcttctgta
     5401 ccactgacgg aaactggcgc acattttcag ttggaagtag ctttgctgaa agaccatgtg
     5461 atggtaactc tggatacgac aggaccaagt ttattcaaac gtggttatcg cttagaaaaa
     5521 ggtggagcac cattgaaaga aaacatggca gcagcactta ttatgttgac aaattggcga
     5581 aaagaccgtc cattttatga tcctgtatgt ggttcaggta cgctttgtat tgaagcagcc
     5641 ttaataggcc ataatatcgc accaggattc aatcgtgagt ttgcttgcga atcttgggat
     5701 tggttcagtc aagaaatcat ggaaaatgtt cgatcagcag ccgaagaaaa agcagattat
     5761 gatattgaat tagatattac tggttcagat atcaatggac ggatgattga aattgccaaa
     5821 gccaatgcag aagaaatcgg attaggctct tcgattacct ttaagcaaca agcagtcaaa
     5881 gacttcaaaa cggataaaga atacggggtc atcgtggcta atccgcctta cggagaacgt
     5941 ctaggagaag aagaaactgt tcggaaatta tataaagaaa tgggcgatgt attccgtcca
     6001 ttaaaaacat ggagtaaata tatcttgact agcgacttag cgtttgagga attttacgga
     6061 caaaaagcaa ccaaaaaaag aaaattgtac aatggtgcat tgcgtacgga tctattccag
     6121 tattggggaa ctcgtccgcc tagaaaaaaa gagaaagacg aataatctta ctaattgatt
     6181 cattcaattg gatgtttgga ggaataggga atgaaagaaa aagagttttt acaagaagta
     6241 aaagaaatca atcttctaca acaagcttta ggaatcctcg attgggatac acaaacaggt
     6301 atgcctgaaa aagctagtgc ataccgaagt gaagtagaca gctatcttta cagcatttat
     6361 ttttcaaaaa aaatcggtcc aaaaatcaaa gaagccattc gctatttttc tgaacatccg
     6421 gaagaattgt cggaagttgg aactgccgtt tttgccaaag taaaagaaga gtatgatttg
     6481 gaacatcgcg taccggaaga tcttatgaga aaatatacag ctgccgtttc ttcagcacac
     6541 ggacaatggc aaaaagctag agaaacacaa caattttctg attttcaaca cgcattgaat
     6601 caaaatattg aattaacaaa acagttgata ccttactgga gaaaagatga aaagacgaat
     6661 tatgatgttt tgctgaatca atacgaaccc gggatgactg tcgaaatcct tgataaggta
     6721 tttgatcagc ttaaagaagg aattctttcg atccgccgta cgctccaaga aaaagggact
     6781 gaacctgaaa cagacttttt aaatcggaga atgacgaaaa aacaacagaa gagatttgta
     6841 gtgaaagtaa tcgaacaact tggttatgat tttagtcgtg gtcgtttaga tgacactgtc
     6901 cacccattta tgactggaat taatccgaat gatgcacgaa tcacgacacg atggaacgaa
     6961 acagatttca aaatggctgt ttttggagtg atccacgagg ctggtcatgg tatgtatgaa
     7021 caagacatcg atgataaata tgcgtataca ccggtttatg aaggtacatc aatgggtatc
     7081 catgaatcac aatcgttgtt taacgagatc attattggaa gtagtcgcag cttctggaaa
     7141 aagcaatatc ctttctttca agaatgtgca gaaggaacat tcgatgatat tccttttgac
     7201 aaattttatc gttcattgaa ccatacaaaa tcaagtttgg ttcgtatcga ggcagacagt
     7261 ttgacttacc ctctccatat catcattcgt tatgaaatcg aaaaaatgat tttcaatgaa
     7321 ggggtagatg ttgctgaact acctgaaata tggaatcaaa aatatgaaga gtatctggga
     7381 atacgaccaa caaatgatgt ggaaggcatc ctacaagacg ttcattggtc aggtgcaagc
     7441 tttggttatt ttccctctta tgcattaggg tacatgtatg cggcacaatt acttcattct
     7501 ttgcagcaag aaatagatgt ggaaaatgtt ctagcgtcag acgattattc tccaatcaaa
     7561 aactggatgt cagagcatat ccatcaatat ggtgcatcta gaaaaccaaa tcaattaatc
     7621 atggatgcaa cacatgaacc gcttaatccg caatacctga tcgactatat gaaagacctg
     7681 tattttaatg tatatcaggt aaaataaaat acaatttaca aagatagctg aaattcatct
     7741 aatctataga tgttttcagc tatttactta agaaagaagt ggaacgaatg gataaattac
     7801 aagtgatcgc tgattatagt atggaccaat tagcgcatga tcaaacagga catgggagcg
     7861 atcataccaa aagagttgtc aagttagctg aaaaaatttt ggagacggaa ccgcaggcag
     7921 accgctttgt gacgcttgca gcagcttacc tacatgatac gattgatgat aaagtcgtta
     7981 aaaatgagaa cgaggcaaag caacagcttc gtgtattcct tcgctctttg ccaatagtag
     8041 aggaacagat ctcgatgatt ttttccatta tcgaaaatat gtccttttct aaaaatctct
     8101 ctgaagcggc cgaactatct ctggaaggta agatcgttca agacgctgat cgtatcgaag
     8161 cattaggagc aatcggcatt ttgcgtactg cttatttcgg cggtggacat gggcacccga
     8221 tttttgatcc tgaactgtat ccgcaaacat tcaaggacaa agaaaattac cgaaaaggca
     8281 cgacagtgat caatcatttt tatgaaaaac tgttcttgct gtctgataaa atgaatactg
     8341 attacggata tgaagaagcc aaacgaagag aaaccttcat gcgtgaattc ttagaagaat
     8401 tttttctgga atggtctatt gacgatgaaa attttactcc agacaattgg atcaaaaata
     8461 acaaaaaatg atggaggttg tgacagaagt ggtcagctcc aagatatttc ggatcatttt
     8521 ccgtaaaagt tacttctgga acactgttta ccaagaggtc gtgagcctgc cgttcagctc
     8581 cgagtcacaa ggaacaattt caaaaaatag cttctcgtgt ttgatgaata ttgtaacttg
     8641 tgattcggaa ttggcaggag aacaccgttt attcggaaga ataggactgt gacaagactt
     8701 ttgtcacagt ccttttttcg aattcaacaa gtaaaacttt taagaaatat tcagagagta
     8761 aaattaaaaa ttgctgataa acgaagatgg aaattttatg tatttttatt ggtctatgca
     8821 ataaataaat taaatattca gaatacaatt gattttaacg aaagggcttc tatcaatggt
     8881 tgtgttgtat ttatctgccc aggtagagta aaatagatca ttatctaaaa ggtaattgtt
     8941 catactatat aagtcatttg aaagtaaaaa gatactgtaa ataaaaaatg tggttaaata
     9001 attctcataa tcagtgtaag cgttatcaaa aatatagcat acttgctttg tagatgaatg
     9061 acacgatata tgtttggtat gttctgcaag acagatgaat atggaggtgg agacaatgtc
     9121 ttccaaagaa ctaacaatgt atttagagaa agaattggat caactgaaag aaaaggggtt
     9181 atacaatacg atcgacatac tagaaagtga aaatggtgct tgtatcatcg tagacggaaa
     9241 aaagatgatc aatctagcat ccaacaatta tcttggattt gccaatcgcg aagaattgaa
     9301 aaaagcatgt attgaagcaa ccgaaactta tggtgtagga gcaggagcag tccgaacgat
     9361 caatggttcg ctgaaaatcc atcagcaatt agaagaaaaa atcgcagaat tcaaagggac
     9421 agaagcagca atcgcctttc aatctggatt caactgcaat atgggcgcta tctctgcagt
     9481 aatgacaaaa gaagatgcta tcttatcgga tgaattaaac catgcatcta ttattgacgg
     9541 ttgccgctta tccggagcaa aaatcatccg aattaaacat caagatatga aagaccttga
     9601 gaaaaaagca aaggaagcga tagaaagtaa gaaatataaa aaaatcatgt atatcacaga
     9661 tggtgttttc tccatggatg gagacattgc acgattgccg gaaattattc cgattgttga
     9721 aaaatatgga ctgatcacct acgtcgatga tgcgcacggc tcaggcgtaa ctggaaaagg
     9781 tgcaggaacc gtcaagcatt ttggattaag cgacaaaatc gatatgcaaa tgggcacgtt
     9841 atcgaaagct ataggtgttg ttgggggtta tgtagctgga tcgaaaacac taatcgactg
     9901 gttgaaagct agaagtcggc cattcctatt ttctacttcg ctaactccag gtgcagctgc
     9961 agctgcattg gcttcgatta ctttgatgca agaacatccg gaacttgtcg aaaaagtttg
    10021 ggaaaatgct aattatttta aagaagaatt gaaaaaagta ggctataaca tcggtatgtc
    10081 tgaaacacct attactccag tcattttggg agacgaaaaa gtgactcaga ctttctctaa
    10141 gaagctgatc gaacatggga tttatgctaa accaatcgtt tatccaactg tgcctcttgg
    10201 gacaggcagg atcagaaata tgccgactgc ggaacatacg agggaaatgc ttgatgaagc
    10261 agtagcagtt tatcaaaaaa taggaaaaga aatggaaatt atttaactaa atttagtttt
    10321 ttcgtctatt taaaattaga gaacatgttc acgcatatat tgttaatcat ctgaagatag
    10381 tagtaaaaaa gatcagggag gatcatcatg aaaaaagtat tggtgacagg atgtttagga
    10441 caaattggtt cagagttgat cacacggcta cgtcaagaac taggcagtga aaatgtcatt
    10501 gcgacagata tacgcatgcc tgaagtcatt gagaaaaacg atatctttga gatcctagat
    10561 gtaatggatt acgaaaaaat gcgcagcata gcagaacaat atcaagtgga cacattgatt
    10621 catttggctg ctcttttgtc agctgttgca gaagcaaaac cacaattagc ctggaatttg
    10681 aacgtgactg ggctggtcaa tgcattggaa gtcgctagag aattccagct gaaatttttt
    10741 acgccaagtt caatcggcgc gtttggtcca aatactccaa aagatcatac cccacaagat
    10801 actcttcagc gtcctacaac catgtatggg gtaacgaagg tagctggaga actactatgt
    10861 gattattatt atgtgaaata tggcgtagat actcgtggtg tccgtttccc tggattgatt
    10921 tcttatgcaa ccttgccagg aggcggtacg acagactacg cagtcgatat ttattattca
    10981 gcaatcaaag aaggaaagta cactagtttc attgatcaag gtacatcaat ggatatgatg
    11041 tatatgccag atgcgatcga tgccattata aaactcatga atgcagatcc tgatcgattg
    11101 gttcatcgaa atgccttcaa tatatcggcg atgtcttttg acccggaaga gatcaaagca
    11161 tctattcaaa aaatcatacc agattttgaa atggattatg atgtggatcc tgtacgtcag
    11221 tcaatagcag aatcttggcc aaattcacta gatgtttctt gtgcgcaaga ggaatggggc
    11281 ttttcgccga aatacgactt agatgtgatg acaaaggata tgttggaaaa actgaaagaa
    11341 aaattggtag agacggtata aaacaagaac cacatccaaa aagtttttta tacttttggg
    11401 attggctatg gtatgaaagg ggacaaggga tggaagaata tcaagccacg ccagtaaaag
    11461 aagtaaaaat agaaaatgaa ttaaaacgta cgatgggatt ttttactgct ttatcgactg
    11521 ttatgggcac agtgatcggt gcaggggtat tttttaaagc cgcaagcgta gcagaagtta
    11581 caggatccgc cagtttgcat atgttttctt ggtttctcgg tggtatgatt tctgtctgtg
    11641 caggactaac aggagctgaa ctggcagctg ctattccaga aacaggaggg atgattaaat
    11701 atatcgaacg gatttacgga aatacagcag cttttttatt gggctgggcg caagtcgtga
    11761 tttattttcc agcaaatgta gcagccttgt cgatcatttt cggtacacag ttcgtcaatc
    11821 tatttggtct ttcccagtca atgattgttc ccgtagctgt tacagctgct gtatcgatcc
    11881 tgttgatcaa ttttttaggt tctaaagctg gaggggcttt tcagtcgatt acgcttgtct
    11941 gcaagcttat tcctttgttt gtgatcgtca ttttcggttt gttccgacaa gaaggagtag
    12001 acttccaact gtttccgatc caagcaggag aaaatctttc atttttctca gccttgggag
    12061 cgggcttgct agcaacgatg ttcgcatacg acggttggat acacgtagga aatatttcgg
    12121 gagaattgaa aaagccggcg aaagatttgc caaaagcaat ttctttagga atcatcggaa
    12181 tcatgatcgt ttatttatta gtaaatgcgg tgttcttaag aaccgcatct atcgacggag
    12241 tcgcaggaaa cagcaatgcg gcaagtgacg ttgcaaaaat gatttttggc ggcttcggtg
    12301 gaagactggt cacagttggt atcttgattt ctgtttatgg aaccatcaat ggctataccc
    12361 ttactggaat gcgtctccct tatgttatgg caaaagaaaa caacctgcct tttagcaagc
    12421 tctttgctaa acttcatgat aaaactaaag ttcctgtagc cgcaggtatc ttagagctag
    12481 tcatcgctat tggcatgatg atgatcggtg gttttgatac actaacggat atgctgatct
    12541 ttgtcatttg gattttttat acgatggtct ttgttggtgt cattcttctt cgtaaaaaag
    12601 aacctgattt gtttcgacca tacaaagttc caatgtatcc gtttattcca ctagtagcaa
    12661 tcattggagg gacttttatc gtatcaagca cgcttatcac tcagaccttt ttagcttcga
    12721 tggggatagt attgacactt gctggtatcc cgatttatct ctatttaaaa cgtagatagc
    12781 taaaacattc gaattttatt agaactgcac ttctctgcca aacaaaatat tttatttaaa
    12841 tgaggagtac gtggaagaaa tgtcaaaagt actcgttaat tttgatcacg taaatgtcgt
    12901 tttgtctacg atgaaatagc aaagtaaaag acagaaatcg ggctcaaccg atttctgtct
    12961 tttactttga tttgcgtaac tgaactattc ggcgtacgcc gcgataaata tttaataaaa
    13021 ccaagcaacc agaaatgaaa atgggaagcc aaatcagata gacagggtag ccaagcactt
    13081 tagacaaagc atgtggagtc attttatcca tttcaagaag tgcttgctgc caagtctgaa
    13141 gattgaagac tagcatgaaa cccgaaataa tcaggagaat aaatcctaaa taatgaattg
    13201 tagttctttt tcttacataa gcgatcgtag cataaaggaa cagtaaaaaa ataggtacgc
    13261 caaaaacaaa gtacatattc agcatctcga cacccccttt gtgtttatct tactttactt
    13321 tgaaagaaat gagaaataat agcctcgtta cttgcttgaa acagcaaagg actgctaaga
    13381 taagagagat acaaagaatc aaggaggagt catatggcaa gttatatcga accgattcgt
    13441 tttgcgctga ttgtttttcc atttttagcc ttagcgatct catctgtttt ttttatttat
    13501 gaatatcgaa agtatggaac atttctagta acaagggcag ttattttgta ttctttcgtt
    13561 ttttatttat tgtgtgcgta ttttttggtg atcttaccac ttccggcaaa ggagacggtt
    13621 gcccaaatga cagggccaaa gatggagctg catttatttg cttcatggca acatttcaga
    13681 gacaacacgg tattggtctt gtctgaacct agtacctacc tgccggcaat gaagcagagt
    13741 gtatttttgg aaccagcttt taatgtttta ttactcatac catttggcat ttatttaaga
    13801 tattatttcc ggctttcttt tatgaaaacg atgattgcta gtttctgcct ttctctattt
    13861 tttgaattga cgcagctaag tggactttat tttatctacc caagaccata ccgcttattt
    13921 gatgtgaatg atttagtgac aaatacattg ggtggaatca ttggttttgt cgttacgccg
    13981 ttatttacat ttatgcttcc tacaagaaga cgaatggatg aactttctta tgaaaaagga
    14041 aagactgtct cattgactcg acgcttggtc gcttatttga tcgactggac atttttatct
    14101 cttattaatt tggtagcggc gattttcttt aaaattgcgt tcgattcagg ggcagttact
    14161 tctcatatct ggtggctttt attagaagta ggggtatact ttatgattgt accgtttata
    14221 accaatggat ggacacttgg caaaaaaatt gtgcgtatcc gtttggtgga agaagaccgc
    14281 gaacgaattt ctttaaaagc attgatgatc cgctacgggt atctgtactt tcttttttat
    14341 ggtttaagct ggttggggca gttgcttcag caaatcatga attcgacaac gaatacggtg
    14401 ttgcagatga gtgcagtatt catttctctc ggtgtcttgt tactacaagg tatttttatt
    14461 ttaaatatct tgctatcatt tttgagaaaa aaacggattc tgttttatga aaaagctagt
    14521 cacacatata ctgttagcac gattggccaa acaaaggatg atgattctcg aaattagctg
    14581 agcaaaaacg ctttgtctgt gttataatgc caaagtcgtg ataatttaaa gagaagaggt
    14641 gcacgaatga ataattttga tatattccgc ttcaaaccgt ttatcaatga agcgttaacc
    14701 gcaaagggct tcaaacagcc cacagaggtc caggaacgtt tgattccttt aattgcaaaa
    14761 ggaaaaaata tcattgggca atctcaaact ggtacaggaa aaacacatac gtttttgttg
    14821 cctttgtttg aaaaaatcaa tgcagaaaaa gaggaagtac aagttgttat tacagctcca
    14881 agtagggaac tagctgcaca gatctatgaa gcagctacac aaatcgctaa attttctaca
    14941 ccggagatcc gtgtctcgaa ttttgtcggc ggtacagaca aacagcgtca attaaatcgt
    15001 ctgcatcacc aacagccaca ggttgtagta ggcacgcctg gtagaatatt agacatgatg
    15061 aatgaacaag cgctgaagat tcacaccgca tttgcatttg ttgtcgatga agcggatatg
    15121 acattggata tgggtttttt aaatgaagta gaccaaattg caagtcgatt accagaaaag
    15181 ctgcagtttt tagttttttc tgccactatt ccagaaaaac ttcgtccatt cttacgaaaa
    15241 tacatggaaa atccaatcat tgaagaaatc aaaccaaaag cagttatttc tgaagacatt
    15301 gaaaattggc tgatttcaac aaaaggtaaa gatgagaatc aaatgattta tcaactgctg
    15361 acaattggtc atccttattt agcaatcgta tttgccaata caaaacagca tgtggatgaa
    15421 attgccgatt acttgaaaaa ccaaggcttg aaagtagcaa agattcatgg agacatcacc
    15481 ccaagagaga gaaaacgagt gatgcgacaa gtacagaatc tggagtatca atatgttgta
    15541 gcgactgatt tagctgctag agggatcgat atcgaaggtg tctctcatgt tattaatgca
    15601 gaaattccag aagatctaga cttctttatc catcgtgtcg gacgtactgg acgtaatggc
    15661 ttaaaaggaa cggccattac gttatattcg ccaaatgatg agcaagccat tgatgcgata
    15721 gaaaaattgg gcgtctcatt tgaaccaaaa gaaatcaaaa acggggaaat cgtcaaaaca
    15781 tacgatcgta atagacgtgc caaacgagaa aaatcaaaag atgtattaga tcccacgctg
    15841 attggtcttg tgaagaagaa aaagaaaaag atcaagcctg gttataagaa aaaaatcgat
    15901 tgggcaattg cagaaaaaaa taaaaaagaa cgcaaaatcg aacgcagaca gcaaagccgt
    15961 actgctcgta aaaataagaa aaattcacat aaataaaaaa caagaggttg tgacaaaagg
    16021 cgtgtcacaa tctcttgttt ttaagctaga ctttttttca ttccgataca aagtacttca
    16081 tcttcaataa aaggttctgt gcaagtatga tacccttgtt tttcataaaa atctacagcc
    16141 gtcatttcag ctactacata actagtctga caaccttctg aaaccgcccg tttttcagca
    16201 gcttcaagca actgaccgcc taaccctttt cctcggtatt cttttgctac gcaaaatcgt
    16261 tccggcttga agcaagtagg tgttttcttt tcataacgca aagtagctac tggtcttttt
    16321 tgatcaagaa atagataata ggtttgttgc gggatatctt ttccgtcgaa ttctagttct
    16381 gcaggaatcc cttgttctaa tacaaaaact tgatatctca aaaagcagct agctgcgcgt
    16441 atccattcat ccgctccaac tatttttttc atcatattcc tccttgtcat gaatagcaga
    16501 gatgtattga ttcctttatt cttatcgtac caaaaatcgt ggagtatgac caaaaggaaa
    16561 tgaaaataaa aattgagata ttgacagtga tcaatagaat gcgtatacta attaagtacg
    16621 aaaaggatat gatttgtttt ttaagactca cagaaaattc tttcttggct gaaaaaagaa
    16681 tagtcactaa tcaaattgct ccctttttct attttggtag aaataccgac gcagctcagc
    16741 gttatcgagt ttaagaggta atgattacac atcattgcaa tcaaggtggt accgcgagtt
    16801 ttcgtccttg gctgtgatgg tgtttttttg tatttaattt atagaaagca ggtaatgaca
    16861 tgaaacaatt aagtagtgca caagttcgac aaatgtttct tgatttcttc caatctaaag
    16921 gacatacgat tgaacctagt gcgtctttga tccccgtgaa cgatccaacc ttattgtgga
    16981 tcaactctgg tgttgcaaca ttgaaaaaat attttgatgg ttcagttgtg ccagaaaatc
    17041 caagaatcac aaatgcccaa aaatctattc gaacaaatga tatcgaaaat gtcggtaaaa
    17101 cagcacggca ccatacgatg tttgagatgc tgggtaattt ttcgattggt gactatttca
    17161 aaaatgaagc catccactgg gcatgggaat ttttgactga tccaaaatgg atggcattcg
    17221 atccagaaaa actttatgta acagtttatc caaaagatac agaagcaaaa cgcatctggc
    17281 atgaagaagt tggcctgcca ctagatcaca ttattgatgt agaagacaac ttctgggata
    17341 tcggtgcagg cccatgtggt ccggatacgg aaattttcta tgaccgtgga gaagcttata
    17401 acgatttagc agaagacgat cctgaaaact atccaggagg agaaaatgaa cgttatttag
    17461 aaatctggaa cttagtattt tcagaattca accataagcc tgataacaca tttgaaccac
    17521 ttcctcataa aaacattgat acggggatgg gattggaacg catggtctca atcgttcaag
    17581 atgcgtcaac gaactttgag acagacttgt ttatgcctat tatccacgcc gtagaaaagc
    17641 taagcaaaca agtaaaatat ggtgaaaacc cagtaacaga tatttcttac aaagtaatcg
    17701 cagatcatat tcgcgccctt tcatttgcta ttggcgacgg tgcattgcca tcgaatgaag
    17761 ggagagggta tgtcttacgc cgtttgcttc gtcgtgctgt catgcatggg aaaaaattag
    17821 gtattgaaca agcattctta tatcgtttag tccctgttgt tggggaaatc atggtaagtt
    17881 attatcctga agtacttcaa caaaaagaat ttattgaaaa agttatccgt acagaagaag
    17941 aacgtttcaa tgaaacaatc aatgaaggtt tggaaatctt aaatcaagtg atccaagaag
    18001 taaaaaacga aaacgggacg actctagcag gaaaagacat cttcaaacta tacgacacat
    18061 atggcttccc ggtagaatta acggaagagg tggcagaaga tgaaggactg aaagttgatc
    18121 atgctggttt tgaaaaagaa atggatgcac aacgcgaacg tgctcgctct gcccgcagta
    18181 ctgaaaaatc aatgggggta caatctgctt tattgacaga tatcaaagta gaaagtaaat
    18241 tcattggata cacagaacaa gaatcagata gtcaattatt ggtcatcgtt aaaggtgagt
    18301 ctcttttgaa tgaagtcgca gaaggtgaag ctcaaatgat ctttgatcaa acacctttct
    18361 atgcagaaat gggtgggcaa gtagcggatc aaggatggat catgaatgaa gtaggcgaaa
    18421 tcgtagcagt tgttacagat gttcaaaaag cgcctaatgg tcaatttatg cacaccgtag
    18481 aagtaaaagc accaatgagt gaaggacaaa cctatcatct attgatcgat caatcgatgc
    18541 gcaatcggat catcaaaaac catacagcaa cccatctact gcacaaggca ttgaaagaag
    18601 tacttggcga gcatgcaaat caagctggtt ctttagtcgc tcctggacat ttacgattcg
    18661 actttaccca ctttggtcaa atcacttcag atgaattagc tgaaatggaa agaatcgtca
    18721 acgaaaaaat ctgggaagct atcccagttg aaacaattga aacagatatc gatacagcca
    18781 aaaacatggg tgcaatggct ttgtttggtg aaaaatacgg acatgacgtt cgagtagtaa
    18841 atattggcgg ctggtcaatc gaactgtgcg gaggcacaca tgtagcaaat acagaaaaca
    18901 tcggaatctt caaaatcgtt tctgaatcag gaattggtgc aggcgtgcga cgaatcgagg
    18961 ctgtaacaag taaagaagcg tacgaactac ttgaagaaga agaaaaacaa ctaaaacaag
    19021 tagcaacaat tgttaaatca cctcaattga aagaagttgt ttcaaaagcg gaacaattgc
    19081 aacagcaagt aagagagcta caaaaggaaa atgaacaatt agctagcaag ttagcgaatc
    19141 aacaagctgg tgatgtattc aaagatccag aaacgatcaa tggaaaaaca atcattgcag
    19201 cacaagtaaa agtgaaagat atgaatcagc tgcgtcaatt agctgaccaa tggaaacaaa
    19261 aaaatatttc agatattctt gttttagcaa acgaacaaga aggaaaagtc agtctgctag
    19321 cggcaatgac gaaagaatca aatgaagctg gactaaaagc tggcgacttg attaaagcaa
    19381 tcgcaccgaa agtaggcggt ggcggtggtg gacgtccaga catggcacaa gctggcggga
    19441 aaaatcctgc tggtattcca gatgcactta aagctgcgaa agaatgggtt gcgcaacaat
    19501 agggcgttta cgtttgacag aaaaacaaaa gttcaaacat tgaacgaaac atgattatgg
    19561 gggcgaagac aaacaagtct tgccctcata cttttttcaa ttggatggcg catctaatca
    19621 aatggtcgga ttcggatggt ctttagttga cgaaagtgac agaaattttt acgattagta
    19681 taaataaaaa ggggtgaaaa aatgaatagt agaaataaga cctttcgact agtcttacgt
    19741 gcgatcttgt tagcaatcat catcgtacaa gcgatggttc cttggctggg gtttattcca
    19801 ttaggattca ttagtttaac aattatccat atcacagtaa ttattgctgc agtagtatta
    19861 ggcccaaaag atggcatggt gattggcttg ttttggggaa tcgctacaat tgttcgtgcc
    19921 tatgccatgc caacaacgcc atttgataca ttagtgttca cgaatccgat tatctcggta
    19981 gttcccagag tacttgttgg cttagtagca ggcttggttt tccactggat ttatcaaaaa
    20041 aataaatcaa taacgatcag ctcagccttt gcaggggtac tgggttcatt ggtcaataca
    20101 gtcttagtgt taggattcat gggattattt tatacaggtg caacggcaga tgcctatggt
    20161 gtagactctg ctcttttatt taaaacctta gcaggggttg cagcaatcaa tgggatacca
    20221 gaagcaatag gtgcgggaat cataacacca ttactagcaa aagcgttatt tgcagcaaca
    20281 ccgttgaaac cagaataatg accatctttt aatagtaata ggacgcacga tagatagaaa
    20341 aaggaagaga ggagaggaaa acaatcgtcc tctccatctt tttacctgag caacaagcaa
    20401 actagaaggg gtaaaaaact tggctaagaa acaagaagaa aaaaagaaac aaataccgca
    20461 acagcggaca aaaaaaggag tactagcggg tatcttagga ttggttacga atctgatctt
    20521 gtttgtctct aagctaatga tcgggctttt ttcaggaagt gtctccatta tggctgatgc
    20581 agtgaacagt ctatcagata ctgcttcatc catattgact ttgatcggat ttaaaattgc
    20641 tgcaaagcca gcagatcagg aacatcctta tggacacgaa cgcttcgaat atatcagtgg
    20701 gttatttgtt tcgatcatca ttacttatgt aggatttcaa tttttagagt cttctgctga
    20761 aaaaatcatc catccagaaa atgtctcttt aacacccatc gtcttttttg tcctaatcat
    20821 ttccatcttt ttgaaatttg cacaaggcaa aatgtatcag gttattgcgc aatccattga
    20881 ttctgaaaca ttgagagcta cagcaacaga tagttataat gatgtcttta ctacactggc
    20941 tgtattgctt tcagcagcaa tcgaatgggt aactggatgg cgtattgatg gatatatagg
    21001 ttttgcttta gcattatata tcctttatag tggattgacg atgcttcgaa attttgtata
    21061 cgaactatta ggaagtcgcc caacgccagc agagattgaa gaaatggaag agcatttaaa
    21121 tgcttgcgat gatatacttg gctatcatga tttattggtg cataactatg gacccaataa
    21181 acgctttgca tcagtccaca tcgaagtaga tgaatcaatg aatttgaatg tggctcataa
    21241 gattattgat ctgatagaaa aagactttaa aaagtctttg gatgtggaac ttgtctgtca
    21301 cttggatcca atgcctgttg ataacgaaaa atactataat atcttgcggc agttaaagca
    21361 aattcttctt gaaacaggta cagggcttaa tattcatgat tttagagtaa ttcatcaagg
    21421 aaaaacgctg caatttgatg tagtggtccc tgagaaattc cagtttgttg atacagaact
    21481 agaaagaatc ttacgtagtc aaatagaaga aaaaatcgga gattaccaat tggaaattac
    21541 gtttgatcac aattatcttt tatattaaat gagaaactat aaaaagactc cttaaagttt
    21601 ttaatttaaa gagtcttttt ttcttttaat tgtttatgga gagcctgacg tgcaagatta
    21661 tctgctcctt tatttttgtt ttcgggcaac cattgcagga tcaataaaga aaattcggat
    21721 agaagttctt ggattctttg aaggtaggga aggaagagct cgttcttcgt tgcgtttttg
    21781 tcgaccgtag aaactagtgt tttgctgtct gaaaaacaga aaatcgtttc attctgtaag
    21841 tcgtttttct ttagaaattc taaagttttt aaaaagactt cgaactctgc ttgatggttt
    21901 gtccctatag aaagcgggat agatagctgt tcttgtttac cttgatagac aatcaagatt
    21961 ccacctccac taggaccagg gttgcctttt gtagaggcat caatgtatac ttttaacata
    22021 aaaagagatt ctcctttttg ttcaaactaa tgttatggta agattatatc attactgaat
    22081 cggtgagagg ttagagaatg aaaaaaaata ttcgatggca gcctgaactg gcacatacga
    22141 tcatctattg gtcattgacc ttgatcattc tgttttttag tctgattctt tctttagaaa
    22201 acacaggtcc ttattggaaa agcaattttg ttttaggtat ttttcttgtt tttctatttc
    22261 taggtttgcg gcgttctttt attttaaaat caaactctgt gattatacgt tatgctgctt
    22321 tttggaaaga ccgagaaata tctgtcggac agattcgatc tttgacgcca actaatactg
    22381 gggtgcaaat gatgttgcaa gatgagaaag ccccatctgt tttcatgatg cgaaaaaaag
    22441 atcaagtagt cctttttgat cattttgaaa aaaacggacc caaatcagaa tcaattcctt
    22501 atccttatac gaaaaaaaga aaaagctaat tttaaaaatc agaacctaaa aaaacgaatt
    22561 tttacaggaa aagaaaagag tgagacgtac ggttcatccc gtttagcgtc tcactctatt
    22621 tgtttatcta ttcatgcata ctagtatcgg tagtctgaac gttgcctttc ttgacatggg
    22681 cagcttgtgt tcctcgattc ccttcgatga tctcaaattc gaccacttga ttttcttcca
    22741 aagttttgaa tccctcttct tcaatcgcag taaaatgtac aaaaatttct tctgtgtcat
    22801 cataactgat aaagccgtat ccttttttgt tgtcaaacca ttttacaatt cctgtggtca
    22861 aagcactcac gacctttcta aagtctgatc atagccttca ttataaagag tatttataag
    22921 tacgtcaaat aaattaaaaa cctgtaattt tatgtttttt ttctgaataa aaaaaggtat
    22981 aattggcata tcttaacaaa gaagatattt tgttgaataa agttcgtgaa aaggggtaat
    23041 caaatgaaga atgatctgca aatttcacaa gaaacagagc tagaaccgat tgttgaagtg
    23101 gctagtaaat tagatcttaa ggaagacgat ttggaactgt atgggaaata taaggcgaaa
    23161 atcaattttt ctggaatcaa tcgatcaaag gataacgctg aggggcattt gatcttagtt
    23221 acttccatca atccaacacc agctggcgaa ggaaagtcaa cgatcactgt agggttagga
    23281 gacgcgctca ataaaatcaa taaaaaatct gttattgcat tgcgagaacc ctcacttggt
    23341 cctgtgatgg gaatcaaagg tggtgctgca ggcggaggat atgcacaggt attgcctatg
    23401 gaagatatca acttgcattt tactggtgac atgcatgcta tcacaacagc taataatgca
    23461 ttatctgctc ttttagataa tcatattcat caaggaaatg aattgaacat cgatgcccgc
    23521 cgagtgattt ggaaacgggt agtcgatctc aatgatcgtg aactgcgtaa agtaattgtt
    23581 ggcttgggtg ggccaatcca aggtgttcca agagaagatg ggtttgatat cacagttgcc
    23641 agcgaaatca tggctatctt gtgtcttgcg gcagaccttg aagatttaaa ggctcgtctt
    23701 gcaaggatcg ttgtcggcta tacatttgac cgtcagcctg tgactgctgg agacttgaaa
    23761 gcagaaggcg ctctggcatt actgctgaaa gatgcaatca agccgaatct tgtacaaaca
    23821 atttacggaa caccagcttt tgtccacggg ggaccgtttg ccaatatcgc tcatggctgc
    23881 aatagtattt tagctaccaa gacagcattg agattagcag attacgttgt cacagaggca
    23941 ggatttggtg cagatcttgg cggagagaaa tttttagata tcaaagtacc taacttgaaa
    24001 aaagcgccgg atgctgtggt gattgtcgcc actgtccgtg cattgaaaat gcatggcgga
    24061 atgaaaaaag atgaacttaa aaatgagaat cttgatgctt tgaaagtagg ttttgcaaat
    24121 ttaaagcgcc atatacgtaa tatggaacaa tatcagctac ctgtgatcgt cgcaatcaat
    24181 gaattcgtaa cagatacaga caatgaactc acactgttgg agcacttgtg tgaaaatcag
    24241 gggattcttg ccaagagagc gagtgtatgg gcagatggag cagaaggcgg cgttgattta
    24301 gcaaaagctg tagtacgatt gattgataga aaagaagcta attacaagcc attataccgc
    24361 ctagaagaaa cgatacaaga aaagacagaa acaatcgtca agaaaattta cggaggaaat
    24421 ggtgttgttt tcagcaaaaa agcgcaaaaa caaatagaag agtttacgaa gaatggatgg
    24481 gacaaactgc cgatttgtat ggcaaaaaca cagtattcat tttctgatga tccttcactg
    24541 ctaggtgcac cggaagattt caccatcacg atccgcgaaa tcatcccaaa attaggggca
    24601 ggatttttag tagccttgac tggagatgtt atgacgatgc ctggtttgcc taaaaaacct
    24661 gctgcattga atatggacgt gacaaatgac ggagaagtgt tgggattgtt ttaagcttga
    24721 ttataatcta ccatcgaagg agctgtattt tgactacagc ttcttttttt agacgatagg
    24781 gaattaattt tgtttacact aggatcattc tttttcttta ttgtattttg agctatacta
    24841 aagcataata ggagggtatg atgaaaacga catttataag aaaaaccggt ttttacggta
    24901 tgggtagctc gattcagcta agaatcaaca atgaaaaacg cagtgttctc agtcatgaac
    24961 aaaagataac gcttgatctg gaagtgccct ttacgatgca ggtcaccttt tattggctaa
    25021 aaagccccgt ttatacagtg aaagaagcaa agcctgctta tgtgatcaca atgaattact
    25081 tgattcttca aatctatccg tttttatttc tagcaactgg gttgggggcc gttttcctac
    25141 aaaatttatt tttcagtttt tttgcagtta ttgtgttgat tgcgttcttt ttctatataa
    25201 aaaaccgggc atatttgatc aaggaggaaa attatggcaa actctgatct ttttttaatc
    25261 agtttcaatc gaatcgaaaa atggatgaga gacgagatgg gaaatgcacg aaatatggga
    25321 tttaccgaac ttgtcagacg cctagctcaa agaaaacaat taatgatcag aaaatacgaa
    25381 gatgatcttt tacagcttgc tcagttaagg aatgcgatcg tccatgatcg gatcgcagtc
    25441 gattttgtca ttgcagagcc aaatgattgg acagtcaagc gaatccagct gatcgaacaa
    25501 gaactgattc agcctgaaac cgtgctgcct cgatttgcaa aacgtgtaac aggttttgaa
    25561 caagatcttc cgttacttga actgttaaag atagtagcag aaaaaaggta ttcacaattt
    25621 ccgttataca ataaaggaaa atttgtagca ttgattactt tacgtaatat cgggttttgg
    25681 ctagcaaaag aaagccaaaa aggtccggtt gatttaacaa ataaacgagc gttggatttg
    25741 atcatccaaa acggaaagta cacaaattat cattttgttc cagctaaaac acatatttac
    25801 gaggttgaag cgatgtttcg tgagcaaggg actttggaag ccgtacttat cactaaagat
    25861 gggaatcccg atggtaatct gctcgggatc gtacgacctc gtgatattta taaacaaata
    25921 gaaaaggatt gattttttgt taattattta ttggatattc agcgctttgc tagtaggagt
    25981 ggatcagttg gtcaaatggt ggatcactga taatctagcg cttggagaga cgaaaaatct
    26041 tataccaggt attttgtcac tgaatcatat tcgaaacact ggagccgcat ggagtatgct
    26101 agaagggaag atgtggttct ttactatagt gacactcatt gcagtcgttg tgattctcac
    26161 attgatgatt aaaaatcgtg aaaaaggtaa tcgctggttc atgattggat tgagtttgat
    26221 tctagctggt gcgattggca attttatcga ccgtattcgt cttggctatg tagtagatat
    26281 gttccagaca gattttatga gctttccgat ttttaacgta gcagatgtga cacttgtatg
    26341 tggagtgatt tgtatgttaa tttatattat tttagatgaa aaggatcaaa gaaaaaaatg
    26401 acagaactta aaacgacgat tcaccatgaa tctggtagga tcgataaagt attgactaat
    26461 ttattcaccg attacagtcg ttcgcaaatt cagctgtggc tgaaaaatgg agcggtttct
    26521 gtcaatggac agccagtcaa agcaaattac aaagtaaaac aaggagacga gattactgtc
    26581 ttgatccccg aaccagaaac attagatgtg gtcccagaaa atatcccgtt gacgatcgtt
    26641 tacgaagatg aagatgtagc ggtgataaac aaacctcaag gaatggtcgt tcatccttca
    26701 gcaggtcata cgcatggcac aatggtcaat gcgctgatgt accatatcaa ggatctttct
    26761 acgatcaacg gcgttattcg tccaggtatt gttcaccgaa tcgacaaaga tacttccgga
    26821 ctgctaatgg ttgccaaaaa tgaccaggca cacgaatcat tagctcagca attaaaagat
    26881 aagacctctt tgcgcaaata tgttgcttta gtccatggag agattcctca cgaaaaagga
    26941 accatcaatg cgccaatcgg ccgttcgaag gaaaaccgga aaatgcaggc gataagagaa
    27001 gatgggaagt cggctgtcac acattttact gtattggaac gtttcgaagg atttactctg
    27061 gttgaattaa aactagaaac cggaagaaca caccagatcc gtgtgcatat gaagtatatc
    27121 ggttatccgt tagctggaga tccagtatac ggaccgaaga aaactttgaa aggcaatggt
    27181 cagtttctcc atgcgcaaat gttaggtttt acgcacccga ctacaaacga aaagatgatc
    27241 tttgaagcac ctcttccaga agtttttgaa aaaactttag aaaaattaag aaaaaacatt
    27301 gcattttaat ttagataacg gtatagtaat taacaacgaa agcataaaac ttaatagaat
    27361 tcctttaagt ttagtcccgt gaggctaaga agggtgggag caaggatacg tatggtgcgc
    27421 cataagtaga ttgctcattg acatagtata gctatcagtg tatcagtata cgctcagtct
    27481 aaacattcct cctgtcctct tagggcagga gtttttttat gcaaaaaagt agggggaatg
    27541 aaaatgcaag caaaagaagt agtagatcag gtaaccatga agcgagcatt gactcgaatt
    27601 acttatgaga ttatcgagag aaaccatagt atccaagata tcgtattggt aggtatcaaa
    27661 acacgaggaa tctacattgc atcaagaatt gccgaacgcc tgaagcagct agaagatatc
    27721 gatattcctg taggagaact ggatatcact ttataccgag atgacaaaaa agaaaatcca
    27781 gaagaaccgg aacttcattc ttctgacatc ccagtttcct tagaaggaaa agaagttatt
    27841 ttgattgatg atgttttgta tacaggacga acgattcgag cagcaatgga cgcagtaatg
    27901 gattttggca gaccaagaaa aatttcatta gcagtcctgg tcgatcgtgg gcatcgagaa
    27961 ttgccaatcc gtgcagatta tgttgggaag aacattccaa catctcgggc ggaagagatt
    28021 ttagtagaga tgcaagagct tgatggacaa gatcgcatca tgattttgaa agaggaagat
    28081 tagaggagag agaaacatgt cggagaaaaa aggatttcat aatgatgacg tggtgttaga
    28141 tatccacgat cgcccaaccc caggacattg ggtaggatta agtttacaac acttgttcac
    28201 gatgttcgga gcaaccgtgt tagtccccat cttagtaggt atcgatccag gtattgcgct
    28261 agtcagttca ggactaggta cattggtgta cttatttact actaaaggaa agatacctgc
    28321 gtacttagga agcagcttcg ctttcattgc agcgatgcag cttttgatga aaacagatgg
    28381 ttatccagcg attgcacaag gagcagtcac aactggatta gtctatttga ttgttgcgct
    28441 gatcatcaaa aagatcggtt cagcatggct ggataaaatc ttgcctccaa tcgtcgtcgg
    28501 tccagtcatc atggttatcg ggttgggact tgcggcaaat gcagcaacaa atgctatgta
    28561 taatcagggc acgtatgatt tcaagtatat tgctgtagcg ctgatcacat taggattaac
    28621 aattttcttc aacatgtggc taagaggttt tctcggattg attccgattt tactaggtat
    28681 tattagcgga tacatcgtag ccttgctgtt tggaatcgtg gatactaagc caatcgtaga
    28741 cgctgcctgg tttgctgttc ctaactttga gattcccttt gtacaatatg aaccaaagct
    28801 atatcttgga gcaatcacaa caatggcacc catcgctttt gtgacgatga cagagcatat
    28861 cggccatctt atggtattga ataaactaac taaacgtaat ttctttgaag aaccgggtct
    28921 tcataagacg ttgacaggtg atggattagc acaaatcgtg gcagcctttg tcggaggtcc
    28981 tcctgtaaca agttacggtg aaaacattgg tgttctggct atcacacgtg tccatagcgt
    29041 gtttgtgatc ggtggagctg cagtatttgc agtcatttta ggattcgtag gaaaattaag
    29101 cgctttgatt ttaagtatcc ctggaccagt catctcagga atcagtttta tcttatttgg
    29161 agtaattgcc gcaagtggaa tgaagatttt aatcgagaac aaaatcgatt ttgataaaaa
    29221 gaaaaatctg ttgattgctt cagttatctt agtagtagga attggcggac tcgtctttga
    29281 agtaggcaca tttacattgt cggcaatggc attagcaact gtacttggca tcattttaaa
    29341 tcttatctta ccagaaactt cacgaagcga agaaaactaa ggaggaaaca ttatgatcat
    29401 caaatcagaa cgtatcagct taaagcattt attgaccgtt gaggcactta ctgatcaaga
    29461 agttatgggc ttgatccgca gagggcagga attcaagcaa ggtgctaatt ggactccgca
    29521 aaagcgccag tactttgcta caaacctctt tttcgaaaat agtactcgca cacataaaag
    29581 cttcgatgta gcggagaaaa aactagggct agaagtgatt gaatttgaag caagcacaag
    29641 ctctgtccaa aaaggggaaa cactatacga tacagtactg acgatgtcag cattaggcgt
    29701 agacgtagcg gttatccgtc atggtgatga gaactattat gacgaactga tacaaagtaa
    29761 aacaatccag tgttcgatta tcaatggagg agacggaagc ggacagcatc caacccaatg
    29821 cttgcttgat ttgatgacca tttatgaaga atttggtcat tttgaaggtc tgaaagttgc
    29881 catcgtaggt gatatcactc actctcgcgt agctaaatcc aatatgcaga tgttaaaacg
    29941 tctaggcgca caagtattct tctcaggtcc tagagaatgg tacgacgatg aattcgaagt
    30001 gtacggacat tatatgccat tggatgaact actagatcaa gttgatgtca tgatgatgct
    30061 tcgggtgcaa cacgaacgcc atgatggaaa agaaagtttc tcaaaagaag gctaccatca
    30121 ggaacatggg ttaacagttg agcgtgcgaa gaagttgcaa aaacatgcaa tcattatgca
    30181 cccagcacca gttaaccgtg atgtagaatt agctgattca ttagtggaag gattacagtc
    30241 gcggatcgtt cagcaaatga gcaatggagt attcgtcaga atggcaatct tagaagcagt
    30301 gctaaatgga aaagcgtaat tataggagga tgaaaaaatg aaaacactca ttaaaaacgg
    30361 tcagatcaat acacgaaaaa atatgacgac accagcagaa atttggatag aagatgggcg
    30421 aatcaaagca atcggtacag gattctcaga agccgaattt gatgaagtgt ttgatgcaaa
    30481 gggacaattg atcacacctg gtttagtgga cgtccatgtt catttgagag agccaggatt
    30541 tacatacaag gagacgatcg aagctgggac tcgctcagct gctcgtggcg gtttcactac
    30601 tgtatgcgcg atgccgaatt taaatcctgt acctgatacg gcagaaaagc tgagacaagt
    30661 atatgacatc attagaaaag atgcagttgt gaaggtattg cagtatgcgc caatcacaga
    30721 aaatctgcga agtgagaaat tagttgatca agaagcactg attgaagaag gtgcttttgc
    30781 tttcacaaat gacggtgtag gtgtccaaac agctgggacg atgtatctgg ctatgaaaga
    30841 agctgcaaaa aacaataaag ccctagtagc ccatacagaa gatgaatctt tattatttgg
    30901 cggcgttatg cacgctggca agaaggcaga agaacttggt cttcccggga tcttaagcgt
    30961 aaccgagtct tctcaaattg ctagagatct gttgctagct gaagcaactg gtgttcatta
    31021 ccatgtttgc catgtttcta ctaaagaaag tgtccgtgta atcagagacg caaaaaaagc
    31081 aggaatccac gtaacagcag aagtttcacc ccatcatctt attttgatcg acgaagatat
    31141 tcccgaagat ttcggctttt ggaaaatgaa cccgccgcta agaggacggg aggacagaga
    31201 agctttgatc gaaggcttgc ttgacggtac gatcgattgc atcgctacag accatgcccc
    31261 ccatggatta gaagaaaaaa gccaaagttt catgaaatca ccatttggta tcgtaggaag
    31321 cgaaacagcg tttcaactga tctatactca ttttgtagaa actggtcgat tcacgttgga
    31381 acaagtgatc aattggttgg cagttaaacc ggctgagatt tttggtttga atgcaggaac
    31441 gctaacggtt ggtgcccccg ctgatgtagc agtttttgat attacacaaa cttgcacgat
    31501 cgataaagaa gactttttat caaaagggga gaatacacct tttatcggct ggaaagtaaa
    31561 aggcgaaaca caaatgactt ttgtcaacgg aaaacttgtg tggcagaagg gagaataaaa
    31621 gtatggaacg tttgcttata ttagaagatg gaacagtttt cgaaggaaag gcttttggtg
    31681 cagatgctag tgtcttcgga gaaatcgttt ttacaacaag tatgactggt tatcaagaaa
    31741 ctatcactga tcaaagtttt aacggtcaaa tcattacatt tacctatcct atggtgggta
    31801 attatggggt aaatagagat gattatgaat cgatttctcc aacatgcaaa ggagtagtag
    31861 taaaagaaca tgcacgtgta gcttcaaact ggcgaaacca aatgacgcta gattcatttt
    31921 tgaaacaaaa aggaattcct ggaatatcag gtatcgatac gcgcgccctt actcgcaaaa
    31981 tccgtgaagt cggaacgatg aaagctagta tagttgatgc tggagattcc ttcgaacatg
    32041 catttgatca gctgaaagca accgttctag caaccaatca agtgcagcaa gtctcaacta
    32101 acaaacctta tccaagtcca ggaacaggtc ggaaagtcat tgtcattgac ttcggcttga
    32161 aacatagtat tttacgagaa ttgagtaaaa gaaactgtaa cctcacagta cttccctata
    32221 acacagatgc cgagacgatt ttagcgctat caccagacgg agtcatgctg acgaatggac
    32281 ctggtgatcc aaaagatgtg ccagaagcaa tcgaaatgat caaaaaaatc caaggaaaag
    32341 taccgatttt tggtatttgt ctcggtcacc agttatttgc tttagccaac ggtgcagata
    32401 cctataaaat gaaattcgga catcgaggat taaatcaccc ggtacgagaa gtagcgaccg
    32461 gaagaattga ttttacttct caaaatcatg gctatgcagt cgatgaagcc acgattgatc
    32521 cagaaaaact catcgtaact catgtagaag taaatgacgg aacagtagaa ggagtcaaac
    32581 acagagatta tcccgctttc agtgtgcagt ttcatccaga tgctgcccct ggtccgcatg
    32641 atgcactaca tttatttgat gaatttatgg aaatgatgga cgcaggaaag gagcagtaaa
    32701 cagatgccga aacgtacaga tatcaagaaa attatggtga ttggatctgg tccgatcatc
    32761 attggtcaag ccgcagaatt tgactacgca ggaacacaag cctgccttgc cttaaaagaa
    32821 gaaggatatg aagtggtttt agtaaattct aatcctgcga cgatcatgac ggataaagaa
    32881 gtggcagatc aagtatatat cgaaccgatc acacttgaat ttgtatcaag gattcttcga
    32941 aaagagcgtc cagatgctct ccttccaact ttaggcggac agacaggcct caatatggca
    33001 atggagctgg cagcatcagg tattttagaa gaattgaaca tagaacttct aggaacaaag
    33061 ctttctgcga tcgatcaagc agaagatcgt gatctattca aacagttgat gaaagaattg
    33121 aatcagccaa tccccgagtc tgaaatcgtc aatacagtag aagaagctgt agattttgca
    33181 aataaaatcg gctatcctgt catcgttcgt ccagccttta ctttaggcgg aactggtgga
    33241 ggtatgtgtg aaaatgaaga agaattacgt atcgtatcag aaaatggatt aagtctttcc
    33301 cccgctaccc aatgtttgat tgaacgaagt attgcaggtt ttaaagaaat tgaatatgaa
    33361 gtgatgagag attcggctga taatgcaatc gttgtctgca atatggaaaa ttttgacccg
    33421 gtgggtatcc atacaggtga ttctatcgta tttgccccta gtcaaacatt gtctgaccat
    33481 gaatatcaaa tgcttcgtga tgcttctctt tcgattattc gagcacttaa gatcgaggga
    33541 ggctgtaatg tccaactcgc tcttgatcca catagtttta attattatgt gattgaagtc
    33601 aatccgcgtg tttctcgttc atctgcgtta gcaagtaagg cgactggcta tccgatcgct
    33661 aagctagctg ctaaaatagc gcttggatta acattagacg aaatgaaaaa tcctgttaca
    33721 ggaacgactt ttgcagaatt tgaacctgca ttagattatg ttgtagctaa aattcctcgc
    33781 tggccgtttg ataagtttga aaacggagaa cggcttttag gcacgcagat gaaagcaact
    33841 ggtgaagtga tggctatcgg tcggaatatc gaagagtctt tattgaaagc tgtgcgttct
    33901 ttagaaatcg gtacacacca tatcgaactt cctgagttgg cagacatcac ggacgatgac
    33961 ctaatccaaa aagtggttcg agcacaagat gatcgcctgt tttatgtagc tgaagctatt
    34021 agaagagggt acaccataga agaattagcg gatttgacaa aaatcgattt attcttttta
    34081 gataaactgt tgcatatcgt agaaatcgaa cagcagttga aacagcagcc gttggatgac
    34141 aagatattga aaacagcgaa acaaaacggt tttacagaca aaaaagtggc ggagctgtgg
    34201 gaaataaacg aagaagccgt tcgccagatg agaaaagaaa atgatttgat tcctgtatat
    34261 aagatggttg acacttgtgc agcggaattc gaatcaaaaa caccttattt ttatagttcc
    34321 tatgaatatg aaaatgaaag ttcacgatca gaaaagccct caattctagt actgggatct
    34381 ggtcctatcc gtatcggaca aggggttgaa tttgactatg cgactgtgca ttctgtcaaa
    34441 gcaatccagc aggcaggata cgaagcaatc atcatgaaca gcaatcctga aacagtttcg
    34501 actgactttt ctatttcgga taaattatat tttgagccat tgaccttcga agatgtgatg
    34561 aatgtgatcg agctagaaca gccgatagga gtaatcgttc aatttggtgg tcagacagca
    34621 atcaacttag ccgaaccttt gactgctgct ggtgtaaaaa tcttagggac aacgatcgaa
    34681 gacttggatc gtgcggaaaa tcgtgattta ttcgagcaag cgctgaaaga gttggatatt
    34741 cctcaacctt taggggatac tgctacaagt aaagaagaag ccgtggttat cgcttcgaga
    34801 attggttatc cagttcttgt tcgtccaagc tatgtattag gcggacgtgc gatggaaatc
    34861 gttgaaaacc agcgagattt agaagattac atggaacatg ctgtgaaagc atctccagaa
    34921 catccagtac tagtggaccg ttacttgctt ggtggtgaat gcgaagtgga tgcgatttgt
    34981 gacggtgaaa cagtactcat cccagggatc atggagcata ttgagcgtgc tggtgtccat
    35041 tcaggggatt caatggctgt gtatccacct caaacattat ctgaatcgat caagaaaacg
    35101 atcgaagatt acacgatccg cttagcatta ggtttgaatt gtgtcgggat gatgaatatc
    35161 cagtttgtca tccatgaaaa tgaggtttat gtgatcgaag tcaatcctcg agcaagtcgg
    35221 actgttccat tcttaagtaa ggtgacagga attccaatgg cacaaattgc gacgaaagct
    35281 attttgggag agaaactaaa gacacttggt tatgaaaacg gactttatcc agaaacaaaa
    35341 gaagttcatg ttaaagcacc tgtcttttct tttacgaaat tgcagaaagt tgatacttac
    35401 ttagggcctg aaatgaaatc gactggagaa gtaatgggat ctgacagaca attagacaaa
    35461 gctttgtaca aggcattcga agcttctgga ttacacttgc ctgaatatgg agccgtatta
    35521 tttacgattg cggacgaaac aaaagaagaa gcactagaat tagctaaacg ttttacagaa
    35581 attggctata gtctgatagg aacgaaacat acagctgctt atttcgaaaa aaatggtctg
    35641 gtcgttgaaa cagtagcgaa aatttctgag gaagcagcag aaaaaaatgt agtcgactta
    35701 ataagagatg gtaaaacaca agtggttgtc aatacgattg ataaagatcg gggaaatgct
    35761 tctaaagacg gtttcatcat ccgaagagaa gcagttgaac atggtattcc attgtttact
    35821 tctttagaca cagcagatgc gatcgttcga gtgatggaat cccgtgcttt ttcaactaac
    35881 cctatttaac ggcgagcaca tgttaagaca aaaagacgaa aaaccgttgc aggaggggac
    35941 agatgaaaca agaaatgatg gaaatcgttc agcaaaaaca gctggcacca aagatttttg
    36001 aaatgacatt gaaaggtgat ctagtaaaag aaatgcagca accaggacag ttcttgcata
    36061 ttctcgtgcc gcgtgcagac ttgctattac gccgaccgat tagcttgaat catatcgacc
    36121 acgaaacaaa tacttgccgt atcatttatc gggtggaagg tgaggggact agagtcttct
    36181 ctactctttc tgccggagac catttagatg tcatgggtcc tttaggcaat ggttttgatt
    36241 tgtccaatct tcatacagga gacgaagtat ttataatcgg tggaggaatc ggtgtccctc
    36301 ctctatatga gttatcaaaa cagttagtcg aaaaaggaat taagccaacg cattttttag
    36361 gttttgccac acatgaagtc gtttattatg aaaaagaatt taatcgtcta ggagaaacaa
    36421 gaatcgctac tgacgatgga acatacggta tgcacggaaa tgtcggtaat ttattgttag
    36481 gaacgaagac ggaacctaca gctgtttttg catgtggttc aaacgggtta ttgaagacag
    36541 tggaacagct gttttcttcg catcaaaatg tccagttatc attagaatcc cgcatggcat
    36601 gtgggatagg ggcttgctat gcttgcgttt gtcatacgcc ggaagatgaa acaggaacaa
    36661 aaagtgtgaa agtttgtgac gaaggaccga tttttaaaat aggagaggta attatatgaa
    36721 tgagaatcct ttagcggtca aacttcctgg cctagaatta aaaaatccta tcatgccggc
    36781 aagcggctgt ttcggttttg gcaaagaata cggaaaatac tatgatttga atcagcttgg
    36841 atcgatcatg gtcaaggcaa cgactccaaa tgcccgcttt ggcaatccca cgccaagagt
    36901 ggcggaaaca ccatctggca tgttgaatgc aattggtttg caaaatcctg gtctagacgt
    36961 tgttatgcat aaacttcttc cagaattaga ggagtacaat gagttaccca tcatcgcaaa
    37021 tgttgctggt gcttgtgaag aagactatgt agaagtttgt agcaaaattg gcgaagcacc
    37081 aaatgtgaaa gcaatcgagc tgaacatttc ttgcccaaat gtcaaacatg gcggaattgc
    37141 ttttggaaca gattctgatg ttgcttttca attaacacaa gcagtaaaga aagtttcttc
    37201 tgttcctgtc tatgtcaaat tgtccccaaa tgtaacagac atcgttccaa tcgctcaggc
    37261 aatcgaagct ggaggggcag atgggtttac gatgatcaat actcttcttg gtatgcggat
    37321 tgaccttaag acaagaaaac cgattttagc caatcaaaca ggtggactct ccggaccagc
    37381 aatcaagcct gtcgctattc gtttgattca ccaagtagca agtatatcca gccttccaat
    37441 cattgggatg ggcggcgtgc agacagtgga tgatgttctt gagatgttta tggctggagc
    37501 aagtgctgta gctataggga ccgcgaattt caccgatccg tatatctgtc ctaaactaat
    37561 taaagagctc cctgtgcgaa tgagcgaact aggcatcgaa tcgctggaaa gattaatcaa
    37621 agaagtgaga gaggaacgtg aacgatgaat caactagacc aacgaccaat tattgcatta
    37681 gatttttcaa ctaggcaaga ggtagaagac tttcttcgat ttttcccgaa agaagaaaaa
    37741 ttgtttgtga aaatcggaat ggaattgttc taccaagaag gacctgaaat cgtacgttat
    37801 ctaaaagagg ctggtcataa tgtattttta gatctgaaac ttcacgatat tccgaatacg
    37861 gtcgaaaaag caatgcgggg attggcaaag cttggtgttg atctcacctg tgttcatgcc
    37921 gctggcggta ttcgtatgat ggaggcggca atgcgaggat tagaagaggg gactccagaa
    37981 ggtggaaaac gtccgctact tctagctatc actcagctta cttctacaag tgaagaagaa
    38041 atgcatgccg atcaactaat cgaagtttct ttagaaaaaa gtgtgatcca ttatgctagc
    38101 tgtgccaaaa aagcaggatt agatggtgtg gtttcttctg cttgggaagt agaagcgatc
    38161 aaagaaatgg caggagacga atttgtttgc ttaactccgg gtataagacc agaaggaaca
    38221 gtcgcaggag atcagacaag agtagtcaca ccttctcagg caaaaaagat cggctcaact
    38281 tttatcgtta taggtcgacc aatcactcag tcaactaatc cttataaagc atatcaaacg
    38341 attcaaacag aatggagcta acctaaaatg aatgttgaac aaagtatcgc aaaagactta
    38401 ttagaaattg aagcagtatt tttaaatcca agtaatcctt ttacatgggc aagtggaatc
    38461 aaaagcccaa tttactgtga taaccgaatc acgatgagct atccaaaagt ccgaaaagag
    38521 atcgcaaaag gattagcaag caaaatcaaa gaagcttacc ctgaagtaca agtgattgct
    38581 ggaactgcaa cagcaggaat ccctcatgcc gcatgggtag cggaaatact ggatttgcca
    38641 atggtctata ttcgaagtaa ggcgaaagat catgggaaag gtaaccaaat cgaaggacgt
    38701 atatttgaag gtcagaagat ggttgtgatc gaggatttga tttctacagg cggaagtgtc
    38761 ttggaagcag ctgaagcagc aaaaagagaa ggtgcggata tccttggtgt tgcggctatc
    38821 tttacgtatg agttgccaaa aggaaaagca aatttcgaaa aagccgaaat ccctcttatg
    38881 acgttgacca attattctgt attgattgaa gcagctttag aagatcgtta tatcgatgag
    38941 caagaattaa ctctgctaaa agaatggaaa aaagatcctg aaaattggca gattgaagcg
    39001 cgcttgatga agatagagaa atccgatttg tgtctccaga atcatcaatt ggaatagcaa
    39061 aaatccaaaa actgattgcg agaagcatct tttcatcagg tatgaaaaga aacttctatc
    39121 gtgatcagtt tttgattttt ttaaaattca tttttctaag actggttagc agaaagaaga
    39181 gaataatctc ttctcttttt tctattatat atagattttc tctatttttt ggtaagggag
    39241 ctgtttcaaa aatggtaaag agcggaaatg ttgcgaatat cagttattta atgatatgat
    39301 attcggaaaa agaaatctca aagggaggta acttttatgg ggttgaatga atttatttta
    39361 ggattgaaca atttagagac gatcacaagg gcacctggat tttttaaata tacagagcat
    39421 acagttgcag cacattcgta tcgtgtagca tctattgcgc aagttctagg agacattgaa
    39481 gaagcgaatg gacaaccgat cgattggaaa ctgctctatg aaaaatcatt gaatcatgac
    39541 tacacagagc gttttattgg tgatatcaaa acgccggtga aatacgcaag caaagagtta
    39601 agagggatgc tgcaaacagt tgaagaaaag atgacggacg aattcatcaa ccaagagatt
    39661 ccagctgagt tccaagaagt ttatcgccgt cggttgttcg aaggaaaaga tgaaaccgta
    39721 gaaggagaac tattagccat ttcagataag gttgaccttc tttatgaatc ttttgatgaa
    39781 atcatcaaaa ataacccgga aaaagtttat aaggagatgt tcattgaggc agtgactacg
    39841 attaaaagct ttgaacatcg gccttctgtt cagtatttct ttaaagagat atttccggaa
    39901 ctattgaacc aggaatttta tggaaaagag gcgtttttag aaaccgtctc aacgattgta
    39961 aagaaagaag aaaaataaaa atgatatgag atgcaggggg aagtatagtt gaaaaagaat
    40021 atggatgtcg aatggggcta ttctggagac aaaggtccgg aaaattgggc gagtttatgt
    40081 gattggtttg cgagaggtgc ggaatttcca ttgcagtcgc ctattcattt gaccaagggg
    40141 gaagaaacca aaatagaagg agacacgctt tcctttcatt atcaaaagca acggtttact
    40201 gacaaggaat tcaaaaacac gattcattta gtaccttttg accaactcag ttatgtagag
    40261 ttcaagaaag aacgctatta tttgacagat attcatttcc acttgccttc tgaacatatc
    40321 atcaacggaa aacaggaaga tattgaattt cattttgttc atatgaacag caaaaaagag
    40381 aatctagttg tgggtgtcat gtaccagcta atggtacagg aaggttggct gtataatcaa
    40441 gaaaatgggg aaagttggaa cttagaaaaa catgagcatt gggtaaatcc ggatgtgttc
    40501 tttccggagc aaaaaagcca tttccattat attggctcat tgacaactcc gcctaccagc
    40561 gggccaatcc aatggtttgt aatggatcat gtaggaaaac tgaaccaaga gtttcttctc
    40621 cccttcgatg gtcagtatgc tcgaccgaac aaccgaccaa tccagccctt gaatggaaga
    40681 gaaatttatt atttcgagga gtttgagcaa tgaatattca gcagatgaaa tacgtggtcg
    40741 ctgtagcaaa caatggtagt ttcagagaag cggcaaagaa aatgtttgtc tcccagccaa
    40801 gtctttcagc tggtatcaaa gaattagaaa ccgagttagg gatcacgctg tttactcgaa
    40861 cgaatcgagg ggcatattta acagaagaag ggcaagactt cttaaatcgt gccgagaaaa
    40921 tattggtcca gcttgataca ttagaaactc actatctaac gagtgaaaag agagacaatt
    40981 tttcgatcgc tgcccagcat tatgatttct taggtccttt gaccgcacag ctactcgagg
    41041 aattcaaaga cgagatcaaa caatttcgga tcatggagac gacaacagcg agagtgatcg
    41101 aagaagtcaa agagcagcac agtgagatcg gcattattta tatgaatgag cataatcaag
    41161 ctggtatcag aagatattta caacaaggag agctaagtgg ccgcagcatt ggaaaattca
    41221 aaacgcatat ttttttaagg caaggacatc cattagctga acacgcagag atcaccaaag
    41281 aagaattgtt atcttatcct caagttcgtt ttacacagga tggaaataat ttcccgtatt
    41341 tttatgaaga ccttatcgag atccctgatc aagaaacagt gatttatacg agtgatcgag
    41401 gtactttgat gaatatcgtt cttgaaactg atgcttacgc ttcaggatct ggtattgtga
    41461 ttggagggat tcgcgagcac ttgcggttaa tccctttggc agaaggcgaa ctaaatgaat
    41521 tttatatcat ttattcggcg aaacgtccgc tcagtgaaat cgctcaaaga tttatcaaag
    41581 agcttactcg gttactggac cagcagagct aagaaaagca gatgagaacg ctgtttattg
    41641 aacaccgttg atcaaaaaag aggggttgtg acagaatttt gtcacaactc ctctttttgt
    41701 agcaaacaat ttataactaa ttgctgttgt gggaaagtac gcaaatatga ttctagaaac
    41761 actcattgat tacctgtttt gtttcatagc tgtaattttt tcttcttctg gtgtcacgat
    41821 aatcgtcttt tggttttcat aaataacata accaggcttg gcgccatttg gttttcggat
    41881 atgctttacc tgtacaagat caaccggcac ttgggcagag tatcggtatt ttgaaaaata
    41941 ggcagctaac tcggcagctt ctgtaattgt ttcatctgag ggcttgtcac ttttaatgat
    42001 gacgtgcgaa ccgggtatat tttttgcatg aagccaatag tcggtttttt tagctgtctt
    42061 cattgtcagc tggtcatttt gaagattgtt tttaccaaca agaatgagcg ttccgtcaga
    42121 agaaaagtat tgatcaggct gagatggttt cttacgtttt tgttttttta agttcttctt
    42181 tttcaggtat ccttctgaag tcaattcttc tttaattgct tcgatatcca ttgggccggc
    42241 aatttccagt tgagataaga cagattcaag atattggatt tcatctttgg cttcttggat
    42301 ttgttcgcca atcagcttaa cagcgttctt cagcttttgg tatcgatgga aatatttctg
    42361 tgcattttga tttggtgtca atgccggatc tagtgctatt ttgattggcc gatcttcttc
    42421 ataataatta ggtaaaacaa cttcttttgc gccgcgtggt acttgtgtca gaaaagtagt
    42481 aagtagttcg ccatttctac ggtaattctc agcgttttcg gattctttga gtgtttgttc
    42541 acgttttttc aatttatttt tatttcgttt tagttcattt tcgatttttc gaatcagttc
    42601 tccgccttgt tgttttgccc gatctttctc tgctttttcg tgataatagc tatctaataa
    42661 ttgactgagt gttggataag ctgttacagc agatgcctgt tcggacaata cctgataagc
    42721 aatcggtgtg aaaaaatctt tattttccgt ttcgtaaaaa gtaggaatag gctgatggct
    42781 gatggctgag aaaaattcgt cccatacaac catcttttca ttaggacgtt ccagtaatct
    42841 tttggtcagc tcttgagcga tatcgaaacc gattccctga aactgacgtt ggattgcttt
    42901 tggatctagt tcagtttggg aaagccgctg aaagattttt tctttctcgg agctaaatgg
    42961 gttcaagacc tcttgttttg gcggagcaac atattctaca ccaggaagca aagaacgata
    43021 agtgttttga gagcttccaa tgtgtttgat cgcatccagt atttttccag tttctcgatt
    43081 aactaagacg atcgtactat gacgtcccat taattcaacg atcaatatga tattttgtag
    43141 atcacccaac tcgtttcgtt tagcaaagtg gaaatggatc actcgatcat tttcgatttg
    43201 ttcgatgtct tctaaaatag cgccatccaa atattttcga agcatcatca cgaagttagg
    43261 cggattatca ggattctgat aatcgatttg ggtaatctgt acacgagcat aacttgggtg
    43321 cgcagataat aataatcgat ggtttttgcc gcgtgaacga atcaccagca cgatttcatt
    43381 ttcataaggc tgatggattt tcgagatccg gccagatagt aacgtttcac gcaattcgtt
    43441 gaccatcgcg tgggtaaata caccatcaaa ggacatggtt cattcctcat ttcttttcat
    43501 aagtatattc attataacga aaaaaagaag aaaaaccaat ttggccgaat agtaaagcaa
    43561 aagtctagaa aaatcttgga ctaattacat aaaaaacagt gtttctatcg ttgaaatcca
    43621 aatttttagg t
//
LOCUS       DACDSY010000006        37043 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDSY010000006 DACDSY010000000
VERSION     DACDSY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.006"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(152..1027)
                     /locus_tag="IWK44_000325"
     CDS             complement(152..1027)
                     /locus_tag="IWK44_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9548882.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(1136..2905)
                     /gene="aspS"
                     /locus_tag="IWK44_000326"
     CDS             complement(1136..2905)
                     /gene="aspS"
                     /locus_tag="IWK44_000326"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP9548883.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(2920..4212)
                     /locus_tag="IWK44_000327"
     CDS             complement(2920..4212)
                     /locus_tag="IWK44_000327"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP9548884.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4606..5553
                     /locus_tag="IWK44_000328"
     CDS             4606..5553
                     /locus_tag="IWK44_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9548885.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(5642..6901)
                     /locus_tag="IWK44_000329"
     CDS             complement(5642..6901)
                     /locus_tag="IWK44_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP9548886.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(7072..7518)
                     /locus_tag="IWK44_000330"
     CDS             complement(7072..7518)
                     /locus_tag="IWK44_000330"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP9548887.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(7529..9742)
                     /locus_tag="IWK44_000331"
     CDS             complement(7529..9742)
                     /locus_tag="IWK44_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP9548888.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(9975..10739)
                     /locus_tag="IWK44_000332"
     CDS             complement(9975..10739)
                     /locus_tag="IWK44_000332"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP9548889.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(10740..11687)
                     /gene="prmA"
                     /locus_tag="IWK44_000333"
     CDS             complement(10740..11687)
                     /gene="prmA"
                     /locus_tag="IWK44_000333"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP9548890.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(11700..12188)
                     /locus_tag="IWK44_000334"
     CDS             complement(11700..12188)
                     /locus_tag="IWK44_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP9548891.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(12154..12825)
                     /locus_tag="IWK44_000335"
     CDS             complement(12154..12825)
                     /locus_tag="IWK44_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP9548892.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            12963..14249
                     /locus_tag="IWK44_000336"
     CDS             12963..14249
                     /locus_tag="IWK44_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP9548893.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            14321..14515
                     /gene="ssrS"
                     /locus_tag="IWK44_000337"
     ncRNA           14321..14515
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWK44_000337"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            14593..14877
                     /locus_tag="IWK44_000338"
     CDS             14593..14877
                     /locus_tag="IWK44_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548894.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            14950..15429
                     /locus_tag="IWK44_000339"
     CDS             14950..15429
                     /locus_tag="IWK44_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9548895.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            15651..15794
                     /locus_tag="IWK44_000340"
     CDS             15651..15794
                     /locus_tag="IWK44_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP9548896.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            16372..17955
                     /locus_tag="IWK44_000341"
     CDS             16372..17955
                     /locus_tag="IWK44_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9548897.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(18392..19576)
                     /locus_tag="IWK44_000342"
     CDS             complement(18392..19576)
                     /locus_tag="IWK44_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP9548898.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19601..20608)
                     /locus_tag="IWK44_000343"
     CDS             complement(19601..20608)
                     /locus_tag="IWK44_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9548899.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(20780..21139)
                     /locus_tag="IWK44_000344"
     CDS             complement(20780..21139)
                     /locus_tag="IWK44_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548900.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(21111..21482)
                     /locus_tag="IWK44_000345"
     CDS             complement(21111..21482)
                     /locus_tag="IWK44_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP9548901.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(21499..21801)
                     /locus_tag="IWK44_000346"
     CDS             complement(21499..21801)
                     /locus_tag="IWK44_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548902.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(21818..22288)
                     /locus_tag="IWK44_000347"
     CDS             complement(21818..22288)
                     /locus_tag="IWK44_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP9548903.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(22263..22541)
                     /locus_tag="IWK44_000348"
     CDS             complement(22263..22541)
                     /locus_tag="IWK44_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP9548904.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(22538..23587)
                     /locus_tag="IWK44_000349"
     CDS             complement(22538..23587)
                     /locus_tag="IWK44_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP9548905.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(23544..24473)
                     /gene="cpaF"
                     /locus_tag="IWK44_000350"
     CDS             complement(23544..24473)
                     /gene="cpaF"
                     /locus_tag="IWK44_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP9548906.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            24605..26359
                     /gene="ade"
                     /locus_tag="IWK44_000351"
     CDS             24605..26359
                     /gene="ade"
                     /locus_tag="IWK44_000351"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP9548907.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(26457..27119)
                     /gene="trmB"
                     /locus_tag="IWK44_000352"
     CDS             complement(26457..27119)
                     /gene="trmB"
                     /locus_tag="IWK44_000352"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP9548908.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(27189..27968)
                     /locus_tag="IWK44_000353"
     CDS             complement(27189..27968)
                     /locus_tag="IWK44_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP9548909.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(28175..29386)
                     /locus_tag="IWK44_000354"
     CDS             complement(28175..29386)
                     /locus_tag="IWK44_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9548910.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(29379..30116)
                     /locus_tag="IWK44_000355"
     CDS             complement(29379..30116)
                     /locus_tag="IWK44_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9548911.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            30254..30688
                     /locus_tag="IWK44_000356"
     CDS             30254..30688
                     /locus_tag="IWK44_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP9548912.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            30681..31025
                     /locus_tag="IWK44_000357"
     CDS             30681..31025
                     /locus_tag="IWK44_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9548913.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            31205..32215
                     /locus_tag="IWK44_000358"
     CDS             31205..32215
                     /locus_tag="IWK44_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9548914.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(32284..33228)
                     /locus_tag="IWK44_000359"
     CDS             complement(32284..33228)
                     /locus_tag="IWK44_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9548915.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(33212..35932)
                     /locus_tag="IWK44_000360"
     CDS             complement(33212..35932)
                     /locus_tag="IWK44_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9548916.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(35929..>37043)
                     /locus_tag="IWK44_000361"
     CDS             complement(35929..>37043)
                     /locus_tag="IWK44_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP9548917.1"
                     /translation="VLSNIIEQAIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRL
                     EKNHIPVFIIFGNHDFYQKERYWFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSY
                     RQPWIQKDKVMEFPSRELTDYHIGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGH
                     IHVPTVLNEKQTIVYPGAPQGHTKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKE
                     ISLRKARTTKEVIVHIRQQLSKVEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLL
                     EEFSDRINDFFIWRISLIENTFYTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSH
                     QEAARILSELPEYQEETLEKAKQLLNQDFLFEEDQE"
BASE COUNT        10914 a         7717 c         6541 g        11871 t
ORIGIN      
        1 ttaaaaaaat aaacatgctt tttttcaact ttaagaaaat ttcaaataaa aaagcagttt
       61 ttctttgctg attctactat ggtagaaaaa aactgcagac aaacgaacgt ctgtctacag
      121 cagtatttca tgaatttttg tcaaactcga tctaaatgcc atcctcatag aaacgaccaa
      181 gtatctggac gttttctgca atacttacaa aagcttgtgg atcagaagct ttcattgctt
      241 ctctaagtag aggcagttcg tatcgtgtaa cgatcgtcat caatactgtc tgcttatcat
      301 gcttataagc accttctgct tcattgatga tcgtgatccc tctacgcata cagttttgga
      361 tttcatttac tactttttct ggatgtttcg ttacgatcat tacctgcatc ttcttttgtt
      421 tagtataaac gacatcagtt acttttccac tgacgaaaat agacaacgta ctgtaaaaca
      481 tatattgcca gccaaataga attccggcaa caaaaacgat caatgcattg aagtagatcg
      541 aaatagaacc aatcgacttt cctgtttttt tacgaatcgt gatactcacg atatctagac
      601 cgcccgatga taggccgttt ctcaacgcta gcccaatacc tagtcccatc acagcccccc
      661 caaacaacgc acagatgatt ggatcggtag ataggactgt ttccggtaaa atatggatac
      721 aaagggaggt caaagtgact gtgataaaag tgaaaattgt aaacttcttc ccgattttta
      781 accacgccaa tacaaacaaa ggtatattca gtaaatacaa agttgttgat accggtactt
      841 cgaatcctat cagtgatttt gacaaagtcg tcaaaatctg agctagacca gtaataccac
      901 ttgaataaat atgtcctggc tgataaaaga aattcaatgc gactgatgcg atcaacgcat
      961 aaacgataga taccgaaagc ttggtcgtgt aatcatgata agcccaattt tcgaatgttt
     1021 ttttcatgga aaagccccgc tttatatgtt atggcttcat tataaagtgt ttttcaatta
     1081 aatcaaggtt gtcaaaagaa gcgtattcga caaatccttt ttaccttttt atcggctatt
     1141 cttctatatt cgttacatca atattcaatt cgaacaattg taaaggcgaa accatacttg
     1201 gtgcatcact catcacatct gctgcttttc cgtttttagg aaaggcaatg acttcacgaa
     1261 tattatcttc tccggctaat aacattgcaa aacggtccaa tcctaatgca ataccgccat
     1321 gtggagggaa tccgtaatct aaagctgtta acaagaaacc aaattgttct tccatttgtt
     1381 cttttgtaaa gcctagagtt tctagtactt tttcttgaag ctcacgagta tgaatacgga
     1441 tagaacctcc gcctaattca taaccgttca agacgatatc ataagcttct gcatagactt
     1501 ttgcagggtc ttcagccaat aacgggatat cttcttcttt tggcatcgtg aatggatggt
     1561 gagcagagac atatcgacct tcttcttctg aaaattcaaa ttgtggccaa tcagttaccc
     1621 ataagaaatt gaatttcgat tcatcaatta gtcctaattc ttttcctaaa cgtgtccgga
     1681 tagcacctaa tgcagcagcg acgatttcac ttttatctgc gccaaacatc agcaagtcac
     1741 ctggttttgc atctgttgct ttaatgattg cttctgttgc ttctcccatg aatttagcaa
     1801 ttggaccttt caatccgtct tcttctactt tcagccatgc aagcccctta gctccgaatt
     1861 gtcctacata ttgacctaac tgatccatgt cttttcgtga gtagcgatct gcagcgcctt
     1921 tagcattcaa tgctttcacg acaccgccgt tttcaagagc catttggaag actttgaatt
     1981 ctacttcttt gactgtatcg cttagatcga tcaattccat atcaaaacgt gtatccggtt
     2041 tgtcgctgcc gtagcgagcc attgcttcat cataggtcat acgaggaaat ggcaatgtga
     2101 cttcgatacc gcgaacttct ttcattacct tcgcaatcaa tccctctgtg taagtttgga
     2161 tttcttctgc tgttaagaac gttgtttcaa tatctacttg cgtaaattca ggctgacggt
     2221 ctccacgtaa atcttcatcg cggaaacaac gaacgatttg ataataacgg tcgaatccag
     2281 cattcatcag caattgttta aacaattgag gagattgcgg caacgcatag aaatgtccag
     2341 catggacacg tgaaggaact aggtagtctc ttgcgccttc tggtgtagat ttccctaggt
     2401 aaggtgtttc gatatcgaga aagtcattat catctaaata atgacgaatc gcttttgttg
     2461 tcgcatttct taatttgatg tttttagtca tttttggacg acgcagatcc aaataacgat
     2521 atttcaaacg tatttcgtca ctgattgctt gatcatcttc aatttgaaat ggcggggttt
     2581 ttgcttgatt caaaatcacg atttcacttg caatcacttc gaattcacca gtggccattt
     2641 taggatttac tgcattttct ccgcgtcttc taacttcccc agtcacttcg atcacgtatt
     2701 cactgcgaca tttatccgcg atttcccatg cttctttgtt gatttcagga ttaaagacga
     2761 tttgaacgat tccttcacga tcacgtaggt cgataaagat tactccccca aggtctcgtc
     2821 ttttttgtac ccatcctttt aatgtaactg tttgttctaa ttgttgttcg gaaactaatc
     2881 cgcaatagat cgttctttgt gccatttatt tttttcctcc tactcgaata aagtcatttc
     2941 atcatagact tcatcaaaat gttcatatat ctcttctaat ggaaaacttt tttcttttcg
     3001 gcttgccatt gctttgacat taatcgtttg actagcaagc tcatcttcac cgatcaccaa
     3061 tactaatttg gcattcgctt tatctgctgt cttaaattgc gcttttgctt tacggttcat
     3121 gaagtcgcgg tcagctgaaa aaccaaagtt tcgaatcgct tggaccactt tcaatgcttc
     3181 gatatttgtt tcgtctccga gtgcgaccac gtaagcatcc aactcattaa atgcagggat
     3241 aactacttcc tctgcctcca tagtcaagag taatcgctcg atacccattg caaatccgaa
     3301 gccaggtgtt gaaggaccac ctagttcttc cactagattg tcataacgtc cgcctgcgca
     3361 aatcgttgct tgtgccccca tttttggtgc gtcactcatg atttcaaaaa tcgtatgtgt
     3421 gtaataatct agtccgcgaa ccatattact gtcgatttca taaggaacag ccaatgcatc
     3481 cagcatggaa acaacggtat caaaatgttt ttgagcaggc tcgcttagat aatctaagat
     3541 agatggagct tctgccacaa atttttgatc tcgtttgtct ttactgtcca atacgcgtaa
     3601 tggattttca tgaaggcggc gtttagagtc ttcactcagc tctgattcaa aaggtaaaag
     3661 atagtcgatc aatgcttgac gataggcttg acgcgtttct ttatcaccta atgaattgat
     3721 gactagccgg atttgcgtga tccccaactg tttgaagaaa tcaagtgcca tcaccatgct
     3781 ctccacatca gttgcagggt tttctgatcc aaatgcttct actccgattt gatggaattg
     3841 acgtaaccgt cctttttgag ggcgttcgta gcgaaacatt ggtcccatat aaaaagtttt
     3901 gaacggtttc gcatactctg ggccgaacaa tttatgctca acaaaagaac gaacgattgg
     3961 tgctgtacct tctggtcgaa gcgtcacatg gcgttcccct ttgtcataaa aatcatacat
     4021 ctcttttgaa acgatatccg ttgtatcccc tacacttcgg gatatcactt catagtgttc
     4081 aaaaatcggt gtacgcattt cattatactg ataatctctg aataataaac gtgctgtttc
     4141 ttcgacaaac tgccattttt ctgattcgcc tggcaaaata tcatttgttc cttttggtct
     4201 ttggaaactc atagttttct ctccctttct tagctatgta aataaaaaaa tcacccttgt
     4261 tcatcatgaa caagggtgaa tgaaattcat acacggtacc acctaatttt gaaagatttc
     4321 tctttcctct tacgaataac gctcgtacgc gggacggctt ttcactcgtc catctccaga
     4381 gtgtctttag gctaatgaaa ctttagatca ttttcagcct cgatgatcct ctcttgtaaa
     4441 gtttcaaaaa cttactcgct ctttcattga cattcgatat tacttataac ctactaaaaa
     4501 ccagtctcaa agtcaagaga tatctgacta tttcccctca caaaaacgtt ttagttcagc
     4561 tttttggtat tttgttttag actagacaaa aaggaggaat cagctatggc agaaataaga
     4621 atcggacatt cacaagtcta cgcagaacag cttggattag gagcaaatgc cgttggagga
     4681 cataatcttt ttgacggttt ggaagatgag acaggaaaac aagtagttcg tacagctttg
     4741 aatagcggga tcaatttgat cgataccgct tatgcttacg gaaacggccg ttccgaagaa
     4801 ctgattggcg aggtactgaa ggaaaaagaa tatgatcgct cacgtgtcgt catcgctaca
     4861 aaagctgctc atgtaccaaa taaagggcga acatttgata attctcctga gtttctcaaa
     4921 caatcggtgg aagatgcact aaaacgtctg caaactgatt acatcgacat tttttatatc
     4981 cactttccgg atgaaagcac accaaaaaat gaatcagttg ctacattgca cgaattaaaa
     5041 gaagcaggaa aaatccgagc agtcggtgtg tcaaacttca cgttagaaca attgaaagaa
     5101 gcgaatgcag atggatatgt cgatgtagta gaagataaat acagtttgat ccatcggcag
     5161 gctgaaaaag aattattccc ttatcttgaa aaaaacaaaa tcagttttgt tccctatttt
     5221 cctcttgctt ccggtttgct gacaggaaaa tatgaacttg gtgaggaaaa acaatttggt
     5281 gaaggagatc ctcgaaaaag aaatccagat ttccaaggag aacgtttcag agaaatttta
     5341 accgctgtcg atgtacttcg ccccattgct aaaagatacc aagccactcc tgcgcaactt
     5401 gttttagctt ggtacatgaa gaatccacga gtgtctgtag ttatacctgg agctaagcgt
     5461 ccagagcaag tatctgacaa tgtccaagct ttagatttgc atttatccaa tgaagattat
     5521 caaaccattg atgaagcatt tcgtggattt tgatcaattg accttatcaa tttatagtaa
     5581 tatgaaaaaa ggaagatcag cactgtttga tcttcctttt tttcgataag tttatgctaa
     5641 tctaattctt gtaaagtagc gctcggctat tcggataagt cggaaactga ttgaatggaa
     5701 tcgtccaatc ttgttcaggt acagagaact caaacttatt tactagctga tcggaaaaca
     5761 cacgtagact atgaagagtg atccattctc ctgcacagcg atgcatttgt cgaaaatttc
     5821 ccccgccttg tgcaatcatc tcatattctt ctttatagga aatatctttt gctttcccta
     5881 cataccgttt gatcataaag gaatctggcg cttcaactgt tcgttcatca tggtctgttc
     5941 catataaatc taaaataacc caactgcctt ctggtatacg ataaccatct acttctactt
     6001 ctttcagcga aatcgcaggt agcattggga agaaaggata atagcgtctc atttcttgaa
     6061 taaacggatc ttgtaacgtc gaaaagtcat tttttaattg ctgatacaaa tctggtctac
     6121 tgaaaagtgc atgtcccatc aatgccgccc acacagttag agcaaccgtt ggacggatga
     6181 tgttcaataa ttcaacagct gccacttcca gaggtaaaag ctgaccatct aaatctgtgg
     6241 cattggcaaa agcatataat gccacatttt cttttccagc cacaggattt tgtcgagctt
     6301 cttcaatcaa gccttgcgcc catttttctg attttttgcg attctcgaca ccttttatgt
     6361 gatcaatcgg agaagtaaac gttccgctga tcatagagat ttgatattct gctaattttt
     6421 cgacctcatc tttagaaatc gctgataaat tgatacctgc ccactcgcaa atagatgtaa
     6481 agaaaaccat cttacttaaa tcaaatagtt caaattgacc atgctgtgct tcaagtgctt
     6541 gtgtcagatt cttatctaag atacgatgat aatcttccat tctttccggt gtcattagat
     6601 ccataaaaat atttttccga tggtgatgag cagctccatc caaagtctgt actccacctt
     6661 gaccaaaaag tgtcttcaat actaattttg gcatagcacc ttttcgtttg aaattccttg
     6721 gatcataaaa tacttttgct gctgatgatc cgtagatcgt gatagcttct ttattgaaaa
     6781 tctttgcttt gacgacggga gcgtccgctt catggcgtaa ctcctccaac atgttgtatc
     6841 ctttttgata caattttttt aaatctgtga ttttgatatc tactactggt acttctttca
     6901 tcttcagcaa cctcctataa aaaataactt gtataattta agcttaactt tttttccgac
     6961 tgctgacaaa agaagcgctt tttgcagaca aaaaagccac ttaagttaga gtttatcagt
     7021 ctaacttaag cgacgtgttt catagttttt actagtgaaa accagattac tttatttgtt
     7081 tttagaatca ataatgatcg ttacaggtcc atcattgacg agagaaaccg acatatctgc
     7141 tccgaattgt ccggtagcga ctgtgatgcc gcgacttctg attccttcat tgaatgattc
     7201 gtataaagga atagctgttt ctggtcgtgc ggcttccaca aagcttggtc gatttccttt
     7261 tttcgtatcc gcaaacagtg tgaattgaga gatacttaaa atttgtcctc ccacatcttc
     7321 tacactaata ttcatttttc cttgttcatc ttcaaaaata cgcatttgtg caattttttt
     7381 taccagatag tccacatctt tttgcgtatc ggtttcatga atgcctaata aaacaagtag
     7441 gccgtagcca atttttccaa tttcttgctg atcgactgac acagcagctt tacttacccg
     7501 ttgaacaact gctctcatat ctgtcctcct atccattcgt ccggcgtaca ctgtagacat
     7561 caggtactgc tttgatttta tccacgatac tctttaggtg ggataaattc tggataccaa
     7621 ccgtcaaatg gatcgtcgcc attttatctt tattcgtccg tgcttcaacg ctgttgagat
     7681 ttttcgttag cgcattgact gtttgaagta catcattcaa taagcctgaa cgattatagc
     7741 cgtaaatttc caaatctgca tcatactctt ttcgtgtatt cgatgtatct tcccattcga
     7801 cttcaatcaa acgttctgct acatttggtt tgtcaggctg aacattcgga caatctcgac
     7861 gatgaatgga tatccctctg cctttagtga tataaccaac gatatcatca ccaggaatcg
     7921 gattacagca gcgactgata cgaattagta agttttctac cccttgaatg acaacgccac
     7981 cttcatgacg tactttcatt ttgtcaggtt cttttttcgg ctgattcatg atttcttctg
     8041 cttcttgctt ttgctgctcg attttttgtt ctttacgttc tttttcagtc agacgattgg
     8101 ccatcgtcaa ggggctaact tctccatacc ctacagctgc atagagatca tcttctgttt
     8161 gataattaaa acgatcgagt gcttcttgca gtttgttctt cgtcaaaata tctttaggcg
     8221 taaatcctag atctgtaata catttgacca cggattcgtg gcctttgatc acattttctt
     8281 ctcgatcttg ggctttgaag aaacgtttga tcttatttct tgctttgcta gtagcaacta
     8341 atttcaacca gtcgcgactt gggccaaatg aatttggaga agtcatgatc tcaatgatat
     8401 ctccgttttt caatttgtaa tcaagctgca ccattttgcc atttactttt gcaccagtgg
     8461 ttttgttacc gatatctgta tggatactgt atgcaaagtc caatggtccg gatccttttg
     8521 gtaactcagt aacgtctcct tttggtgtga agacgtagac tttatcacta aagatatctc
     8581 cttttacgcc ttccataaat tcagaagcat catagctttc gtcttgaagt tcgagtatct
     8641 catggaacca gcttaattgt ttcgtcatac catctggttc tactttttca ttttttcctt
     8701 ctttataggc ccagtgtgca gcaaccccga attcagcgat ttcatgcatt tcctgtgttc
     8761 ggatttgaat ttctaccgga tttcctgccg gaccgatcac ggtagtatgc aaagattggt
     8821 acatattagc ttttggcatg gcaatataat ccttaaatct gccgggcatt ggtttccatt
     8881 ttgtgtggat tgctcctaat acagcataac aatccttgat tgaatctacg atcactcgaa
     8941 tagctagcaa gtcataaatt tcgttgaact gctttttctg atctttcatt ttacgataga
     9001 ttgaataaat gtgttttggc cgtccataga tttctgcaaa aatccccagc tcttctgtcg
     9061 ctattcgaat atcttcaaca gtaccactta cgtatttttc acgttcttct ctcttcgtct
     9121 gcatcaagtg gacgatacgg taatactgtt ttggatttag ataacgaagt gctgtatctt
     9181 ctaattccca tttgatccgg ctgatcccta gacgatgtgc aagaggcgca tagatttcta
     9241 aagtttcctg agcgatcctt ctttgcttat cttctcgcag gtgcttcaac gtacgcatgt
     9301 tatgtagtct atcagctaat ttgaccatga taactcgtaa atcttgagcc attgctagca
     9361 gcatctttcg gtggttttct gctagctgct cttcgtggga tttatatttt attttcccta
     9421 atttggttac gccgtccact aacatagcaa cgtcatcgcc aaattcttct ctcagatctt
     9481 ctaaagtgat ttctgtatct tcaacaacat catgtaagaa gcctgtagcc actgtatggg
     9541 gatccatttt taaatctgct aatatacctg cgacttggat aggatgaata atgtagggtt
     9601 cgcctgattt tctaaactgt ccgtcatgtg cagcagtcgc gtattcgcat gctttctcaa
     9661 caaatgctac atgttcggga cccatgtatt gactaacgag ctttatgaca ccgggtcccg
     9721 ttaaaatttc ttcttttgtc acaggaatcg atcccccttt cttttttcgg taaaacggga
     9781 atcgggacaa atatctgttt gataattgtc ccaaaatccg tgcagttata cgttttttta
     9841 cggaaatgtt cgctttttaa aagctttttc aaacgatttt tcgtatccat tgtataaata
     9901 attgtttcta ttatacaaat tgttgactat tttttcaatt ctttctgtcg atactctttt
     9961 attaatgaag aacgttagct gaaagaagga ttcattaatt cccattgata gctagcagca
    10021 cttaataaat aaagaggtgc tgtttctgta cgaaggattc ttggtcctaa tccacaaagt
    10081 atccctttgt gttctacaaa ataatcgact tcatcgcggg ataatccacc ttctggtcca
    10141 aagacagcta agattcgact atctggtttt gtttcactaa gaattttggc tgcatttgat
    10201 ttttcgcctt gctttgctga ttcctcataa gcaaccaaga ccacgtcata atcatctagc
    10261 cgctcaaata aatcttgttt cgtttccaat aactctactg atggcacgat ctgccgatgg
    10321 gattgttccg ctgcctcttg agcaatcttt tcaagccgtt gttgtcgttt catacgtttt
    10381 tttgcatccc atttaacgac agaggctgat gccgggaaac caagaaaagc ttgcgctcct
    10441 aattcagtgg atttctgaac gatccaatcc agcttttccc ctttaggata gccactcgct
    10501 atggtaatcg aacttggcag ttccttttga ttttcttctt ttttaatttc tttcagataa
    10561 acagcctctt cagtaatctc cgtgatttct gcaacaatgg ctatttgatc attgaatgcc
    10621 aaaaaaactt catcctcggg ttccatcctc ataacccgta caatgtggtg atagttttcc
    10681 ccttctaaaa caaaatagtc ttttgattca tatgattctt ttaaaaaata ccgctgcatc
    10741 tattccacct ctggtttttt caaaataatt gcataccagt ctttttgctg gaaaatttga
    10801 tccacgataa acccttgttc taacatggca tccagaacca tttgcttctt ctcttcaatg
    10861 attcctgaaa cgatcagcgt gcctgtttgc ttcagtaaac gccaagcatc tgggatcatc
    10921 aataagatga tatctgctaa aatgttcgca acgattacat cactttcaat ctcgattcct
    10981 gtcaaaagat cgttagctga tacatggaca tctttggcta tttcattcat atccatattt
    11041 tctttcgccg aacgaacggc tacttcgtct aaatcaaagg catagacatc ttttgcacca
    11101 agatattttg ctgcaatact tagcacgccc gacccagttc ccacatctag caatgtttca
    11161 ccaccacgca agactgtttc tagtgctgtc aatgtcaagg aagtcgtggg atgagtgcct
    11221 gtgccaaatg ccataccagg atctagtgtg ataatttttt ctagaggatc ttctgtctgg
    11281 tagttttccc agcttggcgc aaccgtcagc attcgcgtaa tacgcaccgg atgatagtat
    11341 ttcttccagg ctgttgccca gttgctttcc tctacttcac taactgttac tttatttttt
    11401 ccaatagcta gaccaaactc aggtaattgt gtgatacgat ctttgataaa aggcaagatt
    11461 tccggtaaga aagtcgtttc cggaaaataa gccatgacta gtgcgccttc tttcagcgat
    11521 gtaaaatcat ttttatctaa aatctcacca tattgatccg actgaaaatg ttcaacatct
    11581 aaagaatctt caatcgcgac accactcgcc cctgcctcca tcaaaatatt agcaactgct
    11641 tcgactgctt cactttcagt ttctactttt acttcattcc atttcatatc tgtcactcct
    11701 taataacttg ggtacggtaa ataagtatct gcttcatttt gctgttttac ttctactaat
    11761 tctgctagtt gttgattatc ccagtgaagc tcgaatacag cttcgtcatc gtcttcatcc
    11821 aaaaaatcaa gcaaatcgct ttgcccttga gctaatactt catttagata ttccactaaa
    11881 gaatcagcta ctgattttgc caaccctttt tttccttcat aagggattac tgctagataa
    11941 ttgcgttcat caaaaacaga tttctgagga ttaaaaagaa gtacgccgtc ttcaaattcg
    12001 ataattggtt cttgcgaaac gatcccttct gcatcatcaa tctcaaattg tgctttattt
    12061 tctgcaaaca agcgaacaac gatctcgatc gtatgatttt ttgtatccca atctaaagct
    12121 acatcatagt ctgtcacttg tttttcaatt atttcatcta aatatgttaa tagatccttc
    12181 tttttcattt tctttcctcc aaccccaatc tttctcgatt tggttttttc tttgtaaaga
    12241 gatataagga tttcccgaca caacaaaacg caacggcttt tctgtccata cacctttgtt
    12301 tgggataccg attctaggca aagcattgat cttttttggt gtcttttttt tctcaaaaac
    12361 taagcgtaac gaactatctg ctatagactg accataaagt tcttgaggaa ttgctaatgc
    12421 ttccactaat tttcccggac cgttgctgat atctggacca gtttttccgc cacgattttt
    12481 actcatttga tcaatcattg cggcgggttc gattgcacgt atcattactc cttgaggaat
    12541 cccttctggc tgagtaatga tgttcaaaat ccgatgagta tgcatcgtgt aaaggtagat
    12601 cgtacctggt ttttcataca ttgcccgcac acgcggtgtt cttcttaaac catagctatg
    12661 ggcggcctcg tcttctggac ctaggtatgc ttccgcgtct acaatatatc cagctaatct
    12721 tccttctggt gtgtcatgct ccaaatacat ccccaataaa tcccgtgcca cttctggcgt
    12781 cgttttattc caaaaaaatt catctatctt ttgtttatcc gtcatttttc tcacctactt
    12841 tatcatacac aatattagtt ggataagcta tgaaaacaaa acaacagaag gctgttcttt
    12901 ctaggcatct tgctaaatat gatataatta agggacgtaa aaacaagaag gaggaaaatc
    12961 ctatgcaaca gccattggct tatcgcatga gacctcgaac gatcgatgaa gtcgttggtc
    13021 agcagcatct agtcggggaa ggaaaaatca tacgccgcat ggtcgatgct cgcatgctct
    13081 cttccatgat tctatatgga cctcctggta ctggaaaaac gagtatcgca agtgcgatcg
    13141 ccggctctac taattatgca tttcgcatgc taaacgccgc tacagattca aaaaaggatc
    13201 tacaagttgt agcggaagaa gcaaaaatga gtggtacagt gatcctctta ttggatgaag
    13261 tacaccgatt agataaaaca aaacaggatt ttctgcttcc tcatttagaa agtggccgaa
    13321 tcatcttgat cggagcgact actgagaatc cttatattac catcaatcca gcgatccgca
    13381 gccgtacaca gatctttgaa gtcaaaccat taacagaaca agacatccag ttggcagtcg
    13441 aacatgcttt aagggataaa gaacgcggcc ttggacaaca agcaatccag ttagacgaag
    13501 atgctcttct tcacctttcg cgtgcaacga acggcgattt gcggagtgca ttgaatgggc
    13561 tggaactggc tacactatcg actccttcag ataaagaagg aaggattcat cttacactgt
    13621 cgatcattga agaatgtgtc caaagaaaag ctcttaccca tgacaaaaat ggagatgctc
    13681 attatgacgt tatttcggct tttcaaaaat cgattcgtgg aagtgatgtg gatgctgcac
    13741 ttcattatct cgcccgttta gttgaggcag gtgatctggc aagtatttgc cggcgtctga
    13801 tggttatcgg ttatgaagat ataggtttag gtaatcctgc tgctgcggct cgtacagtga
    13861 acgctgtatt agctgctgaa aaattgggat tgcctgaggc acgtatccca ctagcagacg
    13921 tcgtcgtcga tctttgcttg tcgccaaaat ccaattctgc ctacatggca ttagatgcag
    13981 cacttgctga tattcgagag ggcaaagctg gagatgttcc tgatcatttg agagatagcc
    14041 attacaaagg tgcgaaatca ctcaaccgtg gcgtgggtta tcagtatcct catcattttg
    14101 atcaggcttg ggtcaatcag caatatttac cagataaact gaagaatgct cagtattatc
    14161 agccaaaaga tacagggaaa tatgaacagg ctctgggtca acagtattac cgtataaaag
    14221 agtggaaaaa gcatcctcca aaaaactaga caagaaaacg gttctgtttc tgcgagctct
    14281 ctcattgctc tttttaaatt catatgctac tatagaggtg ggaaatctgt gatgttcgaa
    14341 ttgtcctctt tgtgtgttga ccgaacattt ttattgatat cttgggaccc gtcttgtgtc
    14401 actgctggta acatgcctta tcatttggta gttcgaagct acgaccatgg gacccacctg
    14461 cttgaattcg cgggatcaat actacaggac aaacaacggc attaacggag attaccatct
    14521 tttaaagaat aaagcaattt gctcttggca aattgctttt tctatttgtt gcttattttg
    14581 gaggatttgt tcatgattag gattttaatg attatcgcca ctttagtatt attattcgtt
    14641 agctattatt tatttaataa acaggatatc ttttttgtat tgatcaacaa aaatgataaa
    14701 aatcaaggat tcctacaatt ttacggtgct gcatatgctg ttttaggcgt aatgggtatt
    14761 ttaacagctt ttttcaatca acgctttatt gccttggtct ttttactgat cgtaattatt
    14821 gtttctgcca ccttcagtat ccgatttgca aaaaaaattg ctgaaccaaa gcagtaaata
    14881 attctctttt tccctaaagt gttctacaat aaaagagtac aatacgattg acttgaggag
    14941 tgaatgctta tgttacaaca atacaaaaaa atcatggttg ccgtagatgg ttctgatgaa
    15001 gctgaattag cttttaaaaa ggcggtcaat gtagctatcc gaaataacgg agaacttcta
    15061 ttagcacatg tcattgacac acgttctttc caaaccgttt catcctttga tggaatgctt
    15121 gcagaacaag ctactgaaat ggcaaagcaa acattggcag attatgaaag caatgcgaag
    15181 aaagctggct tgaataatgt aacctctgtc gtagaatatg gctcaccaaa acaaattatt
    15241 gccagagaaa tcccagaaga taatcaagta gatttgatta tgttaggagc cacaggattg
    15301 aatgctgtag aacgtttatt tattggttct gtttctgaat atgttattag aaacgcagct
    15361 tgcgatgtgc ttgttgtccg aactgattta gaaaaccaat tgccagctaa agaccacacc
    15421 aaagcttaac acgtagacga cctgccaact atcggcaggt ttttttgcac aaaaaaccgt
    15481 gataaaaaca aaagacgcgg caagcactca tcggtcttta taatgtttaa aataaaggga
    15541 aaacagtcaa tgagttcaag taaaataaaa aaagtgaaca ccagggcgat tagtcgccct
    15601 ggtgtggttt aaaacgacct aagcatgcca aagcttattg gttcgtcaat gtggaagttt
    15661 ccgatgaatc tgatacgaaa aaatatgaag tatcgatggc ggataccgat tttatcgcca
    15721 aactaaaatt ttatttcggg ttcgggtacc gcgagaaggc aagtgctaca ttcggtaaat
    15781 cattcaagaa ttgatgaaag gctaccgctt gccattgcaa ccacaacgat actcctgaac
    15841 gcgatgttgg tgagatttcc aaagagaaga gctgtttaat gcaacggtat taagcaaatg
    15901 gcaaaaggaa gtcatgtaga caaagttttt tatcaaacac catatgattt cgcctgaacg
    15961 ctggtttagc tcagaataca atgatgtggt ccacgagaca ttaccctcag tcagataaaa
    16021 aaagctctct gacagaatta cacatcgata aatattaagt aaatgtcagc gctagttgaa
    16081 ctttctgaca ctttcaggta cactggaaaa gacaactaca cgaaggaggg aaaatactat
    16141 gatgcgcatg actaaaataa caagtaatga aaattaccaa cgataccata tcgcatttag
    16201 ccatgcaatt ggttttacag ttgctgttga cgggacaagt ttgtccaaaa cagttgaatt
    16261 cacgtaaaat caatggccat ctcaaggtat ttttctcatg ataatctgag tttaaataac
    16321 acaggccagg gataaccatg gcctttttga gtggtctcaa ggagagagat catgatcata
    16381 caagcaattt ctattaaaaa aacaatcggc gggaatccat tatttgagga tttatcctgt
    16441 caaattgaag ctggtgaaaa aattgcctta gtcgatatca acggaactgg caaatcaacc
    16501 tttttacaaa tcttgaccgg cagagaaggt gtcgacagcg gtgttatcag tcgcaaaaaa
    16561 ggcttaaaga tcgggacggt cgaacaagaa cttacagttt ccgaagcgac cgtcaaccac
    16621 tacttgctcc attctgccgc tgagatacag gacctcaaac agcaaatgag tcgctacgaa
    16681 gcattgatga cagaaccaga tacaaactta gagaagtgtc tggcccgtta cggtgagctg
    16741 cagcatcgct ttgaagaact aggcggttac gtattagaag accggattgc agcgatcttg
    16801 caaggcttag ggattccaca ctgtcaggaa gcacggttaa cagaacttag cggaggcgaa
    16861 cgagttaaag tcgctttagc caaaatttta gcagccgatg ccgatttact tttactcgat
    16921 gaaccgacca atcatttaga tcttaacagt attcgctggt tggaaaacta tttacagaat
    16981 acaaaaaaat cggttctggt cgtttctcat gatcggcaat ttctcgatca agtcaccaca
    17041 aaaacatggg aactggaaga aggtgcattg attgagtatc ccggaaacta cagccgtttt
    17101 cgagtcttga aagaagctcg cttggctgag ctgacaaaaa attacgaact gcagcaaaaa
    17161 gaagtccagc ggttgaaagt catgattcgt cgttttcgtc agtgggcaca tgaaggagac
    17221 aatgagtcct tcttcaaaaa agccaaagaa ctggaacgcc gcctggcgaa actgacactc
    17281 gtcaaaccgc cacctccccc gaaaaatcgg ctgcaaagct tgtctaatgg tggaaaatct
    17341 ggcaaagaag ttttcatcat tcaaaacttg caccagcagt acgctgatca agtactattc
    17401 aaagacagca gttttgcagt ctatcgtgga gatcatttag ccatcatcgg cgacaatggt
    17461 gcaggaaaat caaccttact caaattatta ctcggtgagg aaaagcctta cggtggcacc
    17521 atcaaattag gtagtagctt gcaaatcggt tatttgccgc agcagctgca gtttgccgat
    17581 cccgatagcc gtttgctggc ttacgccatc acattgactg gaaacgaaga agtcgctcga
    17641 cggcaattag ccaaaagtgg cttttatcaa agcgatgtgg gtaaacggat caaagattta
    17701 tccggtggtg aaaaaatccg tttggcgttg ctgaaactct ttttagaaaa aatcaacgtc
    17761 ctgattttgg atgaaccaac caatcacctc gacagctatg ctcgagaaga aattgaagaa
    17821 atgctgcaag actatcaagg aacgctccta gcagtcagcc atgaccgcta ctttttgcag
    17881 caacacttcc aagaagcact cgttattgat caggagcaga tcagccgcca gccgctcggc
    17941 caactgattg actaggacgt tttttcgcaa caaaaaactt gattttcata cctgtgcttt
    18001 cacgattcag gcaaagtaca ggtggaaaat caagttttta ttattcgatc aatgaatgtt
    18061 tcggatcggt tttctagcta aacataaagc gaagaataaa gtcgacagcc cacgaggctg
    18121 atctaaaaat gttttttctt tttacatatg taatgtccct tataaagatg aatgaaaata
    18181 aaatattggg attttcctga aaatcaataa ataaaatgcc agtcttttcc cttttgacca
    18241 cttttccaag caaaattccc taagaagatc ctgtaaaaaa gaagggaaat tgaagttaaa
    18301 ttaccatttt gcggtcttct ccgcaaaaaa aacacatgca agaattccct cttgcatgcg
    18361 tttcatcttt tatcctaacg aatgctttgg cttagccgcg cagacgctcc acatcacggg
    18421 caatcattag ttcctcatct gttgggatca ctaatactct tacttttgat tcttttgtag
    18481 agatatctac ttcttcacca cgcaattcat tcaacgctgg atcgatttca caaccaaacc
    18541 aagacatacc tttgatgatt tcactacgta catgtccgtc attctcgccg ataccagcag
    18601 taaagacaat tgcatcaaca ccattcatcg ttgtcacata accgccgatg tattttcgga
    18661 tgcggtccac gaaaatgtca taagctaaac gaatatgttc tttttcatag ttcttttcaa
    18721 gatcacgcat atcactagac aatcctgtta ggcctagcag accagatttc ttgttcaaaa
    18781 cattgatcat ttcatcaata tcaatctcta atttttccat taaatatggg agaacagctg
    18841 gatcgatatc acctgaacgt gtacccattg tcaccccagc taatggtgtg aatcccatag
    18901 aagtatccat ggatttacct ccatcgactg ctgtgatcga tacaccattt cctaagtggc
    18961 aagtgatgat ttttagttct tcgatcggtt tgttcaataa ttttgccgca cgttccgaca
    19021 cataacgatg acttgtgcca tggaagccat attttcttac tttgaaatct tcataataag
    19081 acaccggtaa gctatataaa taattttgct ttggcattgt tgagtggaaa gatgtatcga
    19141 atacagccac actcaaaatg tcaggtaaaa tctttttaaa cgctttgatc cccattaagt
    19201 ttgcaggatt atgcaaagga gcaaattctg ctaattcagc aatgcggtcc attactttct
    19261 catcgatcac tactgaatcc ccataagttt ctcctccgtg aacaacacgg tgccctactc
    19321 cggtgatttc atcataagag cttaaaattt gcagttcaat caactgatct aacagcattt
    19381 ttactgctac ttcgtggttt tcgatatcca tgatttgttc gaatttttga ttatcgccgt
    19441 acttgatggt aaaaatagaa tcttgtaaac cgatacgttc gacgatccct ttcgcaacta
    19501 cggtttcttc tggcatttga tataattgcc atttcaaact tgaactccca gcattgattg
    19561 caattgtttt agacatatga ctctccttct cgttgaatga ttacagagca gatgatttcc
    19621 agtttttaaa ctcgttgaaa aattccgtca ccttctctgg ttcttttaat gaagcaagtt
    19681 tcacaagcaa gacttctttt acttgttgtg cttgtggtcc ttttttctgt aagatcaaaa
    19741 tacttttttg cgattgttta ttacggaaca attcatctgg caattgaatc attccttgaa
    19801 gataaccttc ttctttaaac cattttttga tttcttcact ttgatccgtt tctaaaaatc
    19861 cactcggcat caaaaacaaa ccaaaaccat ccggcttcac atatttcatt gattgctcta
    19921 gcaataaatg atgtgcataa ctatggcctt cttcggtact tgttaaaaac tcttttgctt
    19981 tttcgtcatt cggataataa ccgattggca aatcgctgat tgcaaaatcg acaggatcta
    20041 ttaataattc ctgcaacccg tcttgatgaa agtattgaac atttgcttgt gtcaaatcac
    20101 ttgtagaagc tgcgacagcc aacaatgtat catcaatatc cacaccaatt ccttctgttt
    20161 ggtaccccgc attcgatagg tttaaaagaa ctgttaataa caagttgccc attcctgccg
    20221 caatatccag aatcttaacc ggtgcttttt tatttgtgta tagttgttca ataagaaaaa
    20281 caaataaaaa accgatacta tcaggagtga gttgatggtt cgcctgcaaa tgttctgtct
    20341 ggctgccctt caataaaagt aattgagaca gtctgcgcca ttcttctggc tcgaaagaaa
    20401 gtttcttcaa ctcttcataa agagctgtga tccgttgcgt tgtctctttt gtcggcactc
    20461 catccaccac acgtacttga taatcatcta ttaaattttc tgcattttcg acataggctt
    20521 ctaaaaagga agttcccaat gcattctgta aaagctgaat agcttccagg ttttgattaa
    20581 acccttgttc cattttttct ggtaacatgg tgcacctcat ttctagatat attataacaa
    20641 ttttttcacc tgtttaattc tatcgaacaa ttgacaaaat ttcaatcgtt ttcactgttt
    20701 tttatttgtg aaacggcaaa cattttttta ttaaaaacag tgttataaca gaaaagaggg
    20761 cgttttcacg aacattccat cattctgtac tagtatctgt atcatttttt acttcttctg
    20821 tgaaccgaaa gataaacggt gagacttcca cctctaagaa tagggactgt tgtacccact
    20881 gatatgttac agatccagtt gtgtagtaaa aaactccttc cgaactttcg gcaagcgtag
    20941 actgttccga taaagccatc tgcttcatta tttttgctat ataataattt ttgattcgta
    21001 tattaagatc cattgacaaa cggtagcttt ctaatgctgc catcaataaa aacgtacata
    21061 aaagaaataa aagcaagaca gaaaataaaa tagagccctt ataacatttt ctactgatcg
    21121 ttttttgtcc atatggcata tccatcttct ccattttcaa agtgtacata aaaagaaatt
    21181 gtgtgttctt tttccaataa ttgaagctca gtgatacttg ttaacatagg ttgatgtccg
    21241 ccggaagttc ttcttttacg tatcattgcc tgatatcttt cgatgatata ctgtttatcc
    21301 tcgatcgtgt agtgcagttg ctgatttttc acagagacat tgtgactgct cttgagttct
    21361 tcttctaatt gtgctaaaaa tattagccac tctttttgat ctttacgctg caaaaattca
    21421 cttacttgga tggtttgctg aatcaaaaga ttgaatgtga gcaatagact gctcaatatc
    21481 atcagtccaa ttagacattc aatcagcgta aatgctggaa ttacttgttt tttcaatact
    21541 gatcgcttct ttctccatat tgacaaacac tttttcgatt actccattat tatcgacaat
    21601 ttggatatcg tatgggatca tttcttccct tcgctctggt cttttgtgat acatccgctg
    21661 cgtcttgata tcttcataaa tcattcgaat aaatagcagt tcctccccac ttttcttttc
    21721 tgcctgaagc aaaaacagct ggccaaagct cactaagtag acacagacag aagcgatcat
    21781 aagcgctgta agggactcca acaaaacaaa tcctgtttta ttcaattctt tttgtatagc
    21841 gaccactccc tatctggaat tgatacgtaa tcgtccgctc tttttcttcc caataaaatt
    21901 gataagcttt tagtgaactt tcattcccgg ttctagcagc aaaagtaata gaagcatggc
    21961 gtttcaaggt aatctcatca ggaatatcta atacttccca atttatttta tctggattcg
    22021 gaacatgaaa aatgatttga ttctcttcac tattccaata aaagcctgtt tggatctgat
    22081 tcacaatagc gattttttgc gtagaataga ttcgtttttc aaattggccg aaaaattgat
    22141 agactgccaa ttcattcttc catgcagaaa atgcgagaac tggaaaagaa aggatcgttg
    22201 taagtaataa aatcagtaag agtgattcaa acaacgtata tcctgatgct aaaaccttac
    22261 cttcaaggcc gctgatagat ttctgcatgt tctcgtttga tatagccttc attcaacaat
    22321 tcctccacag taggaactct tccattattt tgaagcgcat agagctcttt ttgtgtgtct
    22381 accaactgga tgatcgcttc acgactttct tggtctacat gattcctata acgcgacaaa
    22441 ttagggacga acaacaacac taatacactg atgatcagta agacgattag catttcgatc
    22501 aatgtaaaac cgctataata agatattttt ctctttttca tggcaaaacc tcctctatgc
    22561 ttccgtaaac aggtaataaa acagcggcat aaactaataa aatcaaagcc gctacgccca
    22621 aaaaaatgat tggctgaata aaatgcatcc agtgatgaag acgtttgaaa agctgttttc
    22681 ttgtccattc gctgtaaaaa atcagttctt tacctaaact tccctttgcc tctccctgta
    22741 aaacaatttt actgaattct tctgatagaa aacgataatt ctggaactgc tcagccaatg
    22801 acatacctgc ctccaatcct tgaatcaact tagaggcaag atgctgaatc agactttctt
    22861 gtttcgtttc ctttaagcag actatgattt gccttaattc gaaaccttca tgaaaaagct
    22921 ttccaatctc tagtgacaaa taagccgatt gatacaagga atatatcggt ccaatcaata
    22981 attgtctgct tatccattca ctctgatcaa caggagacaa cttcatcaga cgatacctta
    23041 ctagtaaagc caatagagta accgctagga tgaatagtag aacataccaa tgaaagactt
    23101 gtatcagctg aataccccaa tgctcttgca tgaccattcc tgaaccaata agctgaggca
    23161 aaacgaacaa ccgcatacta aacaagatac ctagcaaaaa ggtgagcaat agcatcggat
    23221 atgcaagcag ctttttcaaa tcattgcgaa atccttcaac aagccgaaac tgtttggcaa
    23281 tttccaacaa agttgtcggc agattcccat gacgttcagc taactcgatc tgtaccagtt
    23341 gctctgctcc atatcctatt tgcgaaagta cctcactaaa cgcttgccct tcctttagct
    23401 gatgttggat atttgccaaa ataacaggtg gaaacgaacg gatcgtgagt aataaataaa
    23461 gggattcttg cagactaaaa ccagataaaa gcagttctcc catggtttgt gcaaactggt
    23521 tttgctggct tcttgttaat cctttagaat agttctgact gccaaatatt ttcctgagct
    23581 tcttcataac tttcctccca tgattttttt actgattctt cctccataaa ttgataactc
    23641 cataaaacag aaggatggtg attggcatct tccaaaagtt cttgataaat gacaccagct
    23701 aaacattcag atagctcttc ttttttcttt gtcagttcat ggattctgac caatgtacct
    23761 tcaagattgc gtgcatgtac ggttgcaaaa acccggtgcc ctgttagtgc tgctcttacc
    23821 gcagcatgtg cagttgattc atctcttatc tcgccaatga ttagcaagtc tggacgatgc
    23881 cgtaatgcga gtttcaaaag tgtatcataa gtctgtgcta ttttcggatt gatctgtagc
    23941 tgcagaaaaa acggttcttc tatctctaca ggatcttcga tagtaatcac ttgcaatttt
    24001 tcttccaaag ccaaccgata cattagagaa gtcttgcctg atccgactgt cccgctgaaa
    24061 aggtataatc cacgtctgac ggttttctgt aaaatagttg caaaatctcg agaactaaag
    24121 caacgatggg gtgtttcaaa agtaccgtat aatagccgaa tcactaaact ctctcgttga
    24181 aggtaatctc ctacagaaga caaccgcaga cgaacttcct gtttttctaa agaataagta
    24241 aatgcaccta actgagcttt tcttttttct ccgatatcca tctgtcccaa atacttaaat
    24301 cttgagatca gttgctgtcc atctgccgaa gagactttct tccagaatgt tcgttttttt
    24361 ccatagcgaa aatagatgta aaatacttct gaagctggca atatatatac gtcttgggca
    24421 tgtttggcaa aactttcact tatcatttga tccgataatc gttttatttc cattattctc
    24481 ccctccacaa aaaacatacg tctttttgat ctttttgttt caaataaaat gaatttacaa
    24541 aaaaatattg gcaaaagccg aatttatgtt atacttataa aaatttatct tgggaggcac
    24601 tccattggaa acaacattaa ggaaatatat cgaagtagca agtaaaaaaa agcctgctga
    24661 cctagtgatc aagcatgcta agatcgtcaa cgtctttacg aaagaaatca tggaaaaaga
    24721 tgtagcaatc tgcgaaggaa tgatcgtagg aatcggtgaa tacgaaggaa aaagcgtaca
    24781 taatgctaat gggcaatatc tagtgccagg attcatcgat ggccatgtgc atatcgaaag
    24841 ctcactttta tcaccaagcg aattttcaaa agtatccttg cttcatggag ttactactgt
    24901 agtgacagat ccccatgaaa taggaaacat tgctgggagt acaggtcttg agtttatgat
    24961 agaagatgca agacaaacac tcatgaatat cttttttatg ctaccttctt gtgtacctgt
    25021 aacccctttt gagaccaatg gtgcaaattt aaatgcaaaa aaattagccc cttttttaca
    25081 aaaacccgaa gttttaggct tagctgaagt catgaactat caagcagtag caaataatga
    25141 aacagacatc ctagaaaaga tccaattgat gcaccagcat aagaaaaaaa ttgacgggca
    25201 tgcagctggt atcggcatgg aagagttaaa tgtttatcct gcagcaggta ttcggacgga
    25261 tcatgaagct acaactgcaa aagaagctaa agaacgtttg gatcttggta tgtatttaat
    25321 ggttcgagaa gggaccgttg caaaagatct ggcatcgcta ctaccggcga tcacacctga
    25381 aaattcaaga agatgcttct ttgtcaccga tgataaactg atcaatgatt tagtaaaaga
    25441 aggatcaatc gatcatatcg tccgtcaagc tattgcactt ggaatcgatc cattacaagc
    25501 cattcagatg gcaacattga atgcagcaga atgttttgat ttgaaaacac taggtgcgat
    25561 tgcccctggg tatcaagctg atttcttctt aacagacgat cttaccaagc tgccaattca
    25621 tgaagttttc tataagggaa cattagttgt tcaatctggg caactccaag accatttatt
    25681 tgctgatcat tcaaattcat atacgtgttg tctgccaaaa ctaaacgctc aaccattaaa
    25741 tccacattcg ttagagctgc ttctttcttc ttcacaagcc catgtgatcg aaattatccc
    25801 gaatagtcta gtgactaaag atttggtaga aaaagtggac cgacaaggcg aacgattcat
    25861 tccttcaatc gaaaaagacc aattaaagat tgcggtgatc gagcgtcacc atcaaacggg
    25921 aaacattgga ttaggcatag tgaaaggatt tcagctgaaa caaggagcga tagcgacaac
    25981 gattgcccat gactcgcata atctagttgt cgttgggaca aacgacgagg atatgctgta
    26041 tgctgctaat cttttgattc aaaaaggtgg cgggatgacc gttgtcaaca aacagcagga
    26101 aatagcttgt gtttctcttc ctataggcgg aatcatgaca caggaacctt tttcgatcgt
    26161 ctatgaacag ttgaatgaat taataactaa agcacatcaa ctaggagcag ctaaaacctt
    26221 tgatcctttt ttgacacttt ctttcttaac attatctgtc atacccgaat tgaagatcac
    26281 cgacaaagga ttattttcat tttctcgctt ccaattgatt ccaccttgtg gaaaaaccga
    26341 tgaaataaat acattttaaa tcaaataaat cccagaaaat acattacatc tgtaatgttc
    26401 attttctgag atttatttat ggatattaat gtttttatga aagcagaaag ttagtttcac
    26461 agacttttct ttttggtctt atcttgaaat tgagcttcta ctcgataaat acgttgacct
    26521 tttgaagaaa atttttcttc atactccgtc ataatattgc cttcaaattg actttgatgt
    26581 aaatctaacc atacctgttt gaggatcatg ccatattgag aaaaacttgc cagagaatac
    26641 tcaaacaatc cttgattatc tgttttgaaa tggatttctc catttggacg aagaatctgt
    26701 tcatctacag caagaaaagt cttatatgtc aatcgccgtt tttcatgacg tttttttggc
    26761 catggatcgg aaaaattcag atagatttga tctacttcgc tgtcagcgaa atactctgtc
    26821 aacgctgatc catccacatg tagaagtttc aggttaggaa gcggttgttc gatcaacttg
    26881 tctagtgcaa tcgaaacaac actgacctgc atttctactc caatataatt gatttcagga
    26941 tgggcttttg ccattccagt gatgaactgg cctttcccca taccaatctc aatgtggatt
    27001 ggatgatcgt tttcaaacaa ttcattccat tttcccttcc acaatgttgg atcactaatt
    27061 acaaaattcg ggtgagccgc taacatttcc gctgctcccg gacgatttcg gacacgcatt
    27121 atttatctcc attcctttcc tctaattaaa aaggtctggc caaatctccg cagagtttgg
    27181 gccaaacctc agccactgaa aattcgtttt aattgtaaaa tttcttcgtt catccgtcga
    27241 tgatcttcac gtttgcaata tctggtgatt tcttggagca aattcatggc cgcataccaa
    27301 tgaaattttt caagcatttc gtcagtcggg cgtatgccat aactgatcag ccactgattc
    27361 cagctagaca gtggcacata atttcctaaa atcgttgcaa tatccaccgc agggtctgca
    27421 aacatgattg aatcccaatc gactagatat aaataatttt tacatactaa ccaattacga
    27481 tggtttacat ctccgtgtac tactgataag ttttccttat ctattgacgg gatctgctca
    27541 cgaagaaaac gatggacaat ttgcagaaat tgatttttct ggagttcttc cggtaattct
    27601 ttttcatatt tttgcagcat cttctccggc gtcatgacac gaccaccgat acgggtcagc
    27661 atggttttaa gagaatttga atgatgtaaa tggtacaaaa catcaatcac gtcgtttctt
    27721 tgaccaatct cattaggagt cagcaagcga ccttccagcc actcttgtgc tgtcaaagta
    27781 tcgccattcc ccgttcgttt tgtccaaaca agttttggtg taattccttc ccttgaaaga
    27841 gcagccaaca tcggagtcgt gtttcgttta ataaagactt tgtcctcatc acgcatgcct
    27901 atgtatgttt ttcctgtgtc gcctttgatg ggacgtaacc gccactcttg gtctaactga
    27961 aactccatca tgcattctcc cgtcctctta ctctttgttt tgcagtactt attattctag
    28021 tcgcctgaac actacacgtc aagcacatat acgttctttt tcatttaatt gccattcaaa
    28081 aaaccagctt ttctcaaaaa aaatagaacg aacagcattc ttactgtcaa cgctctattt
    28141 ttcattctca tttttaccct acacagcttt gttttcagtc tgacatcttc ttcaaacgag
    28201 ttggtacata tagtttaaca aagagcatag taacaatcaa ataagccgct agcggaaccc
    28261 atcgctgctg cccatctaat acgactgcag caatcaaacc aaaaataacc gacgcgataa
    28321 ttaataccca tgtgagtaga tactgaatcg cctgcttctt ctgattctta tggattggat
    28381 aaagctgaac gagaaccata tagcggaact ggttcgctaa aggtaacagt tggaaaccga
    28441 tcaaatataa aaataagaaa ccgataatca gactaaaata aaagtcgact actccggcaa
    28501 tcaataaccc acctataatt actaatcgga aataaagacc actaaaatca gaacctcttg
    28561 ccaatcttct tgcaaacaag taaagataag tgttttttga ttctctttta atatgagtca
    28621 atataggatc caaatagcgt cttcttttga tctttgccgt aatttcagga acatctgtga
    28681 ataaattgat aaattgatag atctgatgca acctatgttc ttctacatgc accattttct
    28741 cccaatccaa tcctttttct tgttgcttcc acaaaaacca gtaatataga cttgcctgta
    28801 ctaatgcacc tgctattcct gcccatatta gccaataaaa tgatagaaac aaaaagatca
    28861 gagaagaaaa aaaccagcct aatttccaat aacgaataac ttgttgatct agtcgaaaca
    28921 attctgctct cttgatttgc agatgactga atttcaaact ccataatatg atcagataga
    28981 cgaaaaacat cgaaaaggat tgctccgtac taaccaccac taatggcatg gccaatccgc
    29041 atgccaatat caaaaagatc aacggaaaaa tgcaagaata gcgaaaagct cgttccaaat
    29101 aggtttgcat ttctttttct ttaggaagta aaaaaacaat atctgctgcc tctgcaaaag
    29161 tagccatctt acccacgaat aaacagagca tccaaaatac agagattatg agccctccaa
    29221 ttgcaaacgg tggctgcagg gttttcaacc aattagaata atataatccc acaccgccaa
    29281 tcaagaaaag acaaactaaa acaaaatgat cgttcaatac ataacgcata tatttcatca
    29341 ttttcttttg atgtcttgct aaacgttttt gaaaaaaact actcatgtgc ctgctccttt
    29401 gttaaagcaa tatagatttc gtccaaagaa gaatccggca gctgaaattc ttttctcaac
    29461 atctcaagag aaccgtctgc tcgtatctct ccgttatgta agaccacaaa acgatcacaa
    29521 tatttttctg cagtagctaa aatatgcgta gacatcaaaa taccggctcc ttcttttttc
    29581 atttctacca tcaattcaag caatgcattg atagctagtg gatccaagcc taaaaatggt
    29641 tcgtcgatga tatataaaga aggctcgatc aaaaaggcgc ataagatcat cactttctgt
    29701 ttcattccct ttgaaaaatt agctgggaac cagtcaagac gattagataa ccgaaaagtc
    29761 tccaacaact tttctgctct tttcattgct atatccatag gtatatcgta ggccatcgca
    29821 gtgacttcga tatgttctct caatgtcaat tcttcatata aagaaggagt ctctggaata
    29881 tacccaattt gtttgcgata ttcttccgta tttttcttta aggatagatg attgatttct
    29941 atcgttcctt ttgaagcttc cagcagaccg ataatgtgct tgatggtcgt acttttccct
    30001 gctccgttca gtccaatcaa ccctactatc tctccaggct gtacatcgaa actgatatct
    30061 tttaacacag ggagatgtcc gtacccccct gtgacatgat cgataattaa actcattctt
    30121 tttcctccgc catttatttt tatttattat accacgaaga aaatgacggt attttgtttt
    30181 tttccttaca atagctttaa cttatggtaa agttaagctg aagtatttat tctttataga
    30241 aaggatgttt tatatggaaa actgtatttt ttgcaaaatc atcaatcaag aaatcccaag
    30301 ctataaaata tatgaagacg acaaggtcta tgctttttta gacatctctc aagcaacgaa
    30361 aggacacaca ttagttgtgc ctaaacgtca tgtagcagat atttttgaat atgatcccga
    30421 acttgcagga gaggtttttt cgcgtgtgcc taagattgct caagcattgg aaaaagcttt
    30481 tccagaaatg aaaggtctta atatcttgaa taacaaccgt gaactagcct atcaatctgt
    30541 ttttcattca catatccatt tagtccctcg ctacagcaaa gaggatgatt tttctattca
    30601 ctttgtcaat catcaagata gttatggatc ggaagaacta aaagctattc aagaaacaat
    30661 cgtaaagcag gtgtcctgcg atgattaaaa actttttcaa aggactgtta tttggagctg
    30721 ctgctggcac tgtcggaggt ctgttattgg cgcccaggag cgggaatgaa acaagaaaga
    30781 aactgatcga agaattggaa gaagcaacaa ctctgacaaa tgatttaaat aacagcctga
    30841 atcattttaa aagcgcattg atcgaaacaa aagaaacagc tgaatctgtg attcctgctt
    30901 tccaagaagc aatcaaaaaa gatatcgaag attttaaatt ccaagccgaa ccacgtatca
    30961 cacaaatcaa tgaacaagta gaaaaactaa aagctgatct gccagagtat cctgaacaaa
    31021 tataataatg attattttct caacagattt aactatataa gtgagaaaag tttgcgtatt
    31081 tcctaggaat gtgatgaaat tttgaatttt gtttgaaact accacgacaa cgtttttaaa
    31141 ggaaatggga tatggtatag ttaatatata aaaataataa ctaattacag gagtgcaaat
    31201 tttaatgaaa aaaaaatcaa ttattttggc agctactagt gcgttggctg ttttaactct
    31261 agcagcttgt tcaggcgata ctaataaaga tatcgcaacg atgaaaggcg gaacgatcac
    31321 tgtatctgat ttctatgatg aagcaaaact tgaatcatcg aaccaatcct tagtacaacg
    31381 gatgatcatt tataaagtat tcaataacaa atatggcgat aaagtaaccg ataagcaagt
    31441 ggatgcagaa tacgacaaac aagcgaaatc attaggtgat acttttgaat cacaattaga
    31501 agctgctgga tatacgaaag atacttataa agaatatatt cgtaacaatt tagcttttga
    31561 agctggcttg aaagctcacg tagatattac agatgacgat ttgaaaactg cttggaaatc
    31621 tttccatcca gaagtagaag cacaaatcat caaattatca agtgaagatg aagcaaaaga
    31681 tgtgaaaaaa tcagcggatg atggagatga tttctctaaa ttagccaaag acaaatcaac
    31741 tgatacagag acaaaagaag atggcggtaa agttaaattc gactcaacga caacaactat
    31801 tcctgcagaa gtaaaagaag cagctttcaa attgaaagac ggtgagatct ccgatgttat
    31861 tactacaaca aatcctactt cttatgcgac agaatactac gttgtcaaaa tggtgaaaaa
    31921 ccaaaataaa ggcaatgaca tggataaata caaagatcaa ctaaaagata tcgctactga
    31981 aactaaacta agtgacaatg cattcacaac taaagtaatc ggtgaagaat taaaagatgc
    32041 aaatgtaaaa atcaaagatg atgcctttga aaatgtgttg agtgcattca caacaacttc
    32101 atcaagtacg aaagactcaa gtgaaactac agcttcaaca aagagttctg acacaaaatc
    32161 aactgattca acaaaagaat cttcaactga agaaacaact gattcatcaa aataaaagaa
    32221 aacaaaaaaa gcgaagactt atgtctcgct ttttttgttt tgaatgaaaa aaataaattt
    32281 ttgctataaa tcttttggta catagaaact tcggttgtcc ataccgaaaa tccgatcggt
    32341 gtattttcct ggttctgttt gacgaatcga agtcaacatc atctgcatcg aagcatcgat
    32401 attgtcgatt tgatgtaata tttctgcttc cattattctt ggacgtacag gtgagccata
    32461 ttctaataag ccatggtgtg ctaaaaccat atgacgtaat acaagaacat cttcgtcagc
    32521 ttcgctgatt ttcaacgcaa gacaggcttt agttatttct tcgtcaatca ataccagatg
    32581 accgatcaaa tttcctgcaa gcgtatattc tgtcgacata ggaccggaaa gttcaatcac
    32641 ctttcctaaa tcatgcaaaa tgataccagc atacaacaat gctcgattca gctcaggata
    32701 ttcatcggcg attgatttcc ctaagtgaag cattgtcaaa gtatgataag ctaatccact
    32761 ggcaaatgca tgatggtttc tttttgctgc cggaaactca aaaaattgtt tttgatattt
    32821 attaagtaga tagcgaacaa tacgattcca atgcgcattg gtgatctcaa acaacaattg
    32881 attgatttct tcttccattt cttctttctt caaaggggct ctttccatgt ataaactagg
    32941 ctctcctggc tcatctaccc ttgttaagcg catatgaaga atctttactt gagggtttcc
    33001 ttgataaacc tctcttttac cgtttagaaa aacgactgtg ccggctgtaa atttattgat
    33061 ttcctcctcc gaagcatccc agtatttacc atcgatagta cctgaagtat cttggaaagt
    33121 aaaagcaata aatttttttc cgtttttagc gattcttaca tctgcacttt tgatcaggac
    33181 aaattgttca aactgctcat cgattactaa atcacgaatt tttttcatta tttgctcctt
    33241 ctaattgttt tacgatttgg ttcatctcat ggtaatagct taccatttct ttatcagaag
    33301 aaaaacaaat tatttgatac ttttctccaa atttagctaa aagttcagct aattgatact
    33361 ttcgctgatg atcataatga agccagccat catcaatgat gatcgggcat atcgaacggt
    33421 tagcttctaa tgagaggtaa gcaaatctca tcgccataat cagctgatct ttcgtacctg
    33481 tagataactc atagatggga aattctgtat atccgtctgt taaagtcagt gtatcatttt
    33541 tcagtaatat cttctgataa tttccttttg ttaaaatacc taaataatca cctgctgctc
    33601 ttaacaactg gggtaattgt ctttcactca tctctgttga aagatcacct aagaatgtcc
    33661 cggcgatttc aagtgctccc cattcctcca gtaattcctg taattttgct ttttgccggc
    33721 tagcttgttg ataaagttcg tccaatgtgc cgtctctttg caactcttct acttgaagtt
    33781 ggagtcgttg ccgattttca cttgcttctt tttcttcttg ctggagttgg gattgctttt
    33841 ctttcagttg gcgcagtctt acagataatt tctcttttgt cagctcttca ggaaatagtg
    33901 gagtaagcat ttccgataat tcattgtatc ttttcatctg ctgttcggct tgtttggctt
    33961 tttgtaaaaa gacaggtact tccgaaggat aagataagcc tgcttgagtc aattgttcgc
    34021 tgtattcttg tatccatact ttttgtttct ttttcaattg attgatttct tgcttcaata
    34081 aagtattttg ctgatagctt ctggcaaatt tcatctgttc catctcttgt ataaaacggg
    34141 aaagcaatac cattttttct gaaattgtct tatcttgtat cggtaaccat tctttaacaa
    34201 actcaaattt ttggtcgatt tgtcgtagtt tttcttgtat ttcctgttct cttttatgtt
    34261 tccgctcaaa tacgccatat aatttctgat aatctatgac ttcctgtcca tattctttca
    34321 tgagtttgag cgtatccatc tttcctaagt gccgatgatc cgcaaaacga tatacttctt
    34381 gctgaagaat ctgtttttct tctccagttc tcttttgttt ttcttctgct tcagctaatt
    34441 gagctaaggc tagatcaagt tgtcctagtt tttcttgcca cattcctttt atctcagaga
    34501 aatcattttt agttttaatt tttggttgcc ttttgtagaa gaaatagcca atcatgcctg
    34561 caccaattag taaaaggatc actttcaagg gcattggtac aaatatacct gcaatgatta
    34621 ttgctccgcc agccatcagc atatacggtt tagatgaaga gactggtgct atctctgact
    34681 gattttgtaa taatgctgga taggtctctt ccaagtcatt cacttctgct tcaatcatct
    34741 cttgctgttc tttagctatt tggagacgca cagaaatctg gctttctaga tcagcaatcc
    34801 ttctttcttc agccagaaga tgcttccatt cttcttcata taaaaattct tccggtaacg
    34861 gatttttctg ccaatgccac ctttgttcca aaatcttcaa ttctgaattt gtttctctag
    34921 tctcaatttg taatctttgc cattcttcgt ttaattgttc cgctgtaaat tgaagttgct
    34981 ggagatcttt tagttcctgc tcctgctcta aataaaaata atatctggct gactgctgat
    35041 ccatcccgct atgcttttcc agtgttgctt ctaattcttc caaacgctca tttgtttgct
    35101 gataccgttt ggagaaagac tccagatcta aaagaagttg ctcatcagga agacctgtag
    35161 tgctgttttt tttgagagat tgccattctt catataaaga aaaattaagt tcttgctgtc
    35221 tggctaatga aaattgtttg ttcgcctctt ttaattgtgc caataactgc tgctgttgcg
    35281 actcgtattc ctgttcttgt tggatcaact tgccaaaata agcttcctgt tcttgttttt
    35341 gatgaatttt ttcttgcagc tctttccatt catttaattt ttgattgatt agtaattttt
    35401 ggcctttttt cttgaataat tgttgtgctt tctgataata ttcttgccgc ttttggaata
    35461 cttgagaact gcccgtgatg cctaaagaaa tcagcgcatc atgtagctct ttttctctca
    35521 aatgatctag ttccatcaac tgctcttgct ggaaagtaaa tacctgctgg aataactctt
    35581 tcgtcaaagg tgcgatcagt ttttctagta actcatcact tcccacttga tcccccagcc
    35641 acaccttcga ttttccgcga ttttgctgct tgtagcgctc gacagcaaat tcaccatata
    35701 caggatgttt aagccatatt ttgccgccat aggctgagcc gtcttttggt gtataatcct
    35761 tttttttacg gccttttgaa gggaaaccaa ataatattgc ttgaataaat tgataaatcg
    35821 ttgattttcc tgcttcgttt cccccataga tcagctgatt gcctgaataa aagtcaaaac
    35881 ttttttgacg ccattttcca aaagcagcaa tctcgatcct taaaatcttc attcctgatc
    35941 ctcctcaaat aaaaaatctt gattaagcag ctgcttggct ttttcaagtg tttcttcttg
    36001 atattccggc agttcgctca atattctagc cgcttcttga tgactatata cttcatttaa
    36061 aatttgttgg aaatcttgag gtgtctgata gtattgaaat aactgttcca tcaatttagc
    36121 agaagcagca agtggggtct tcgtataaaa agtattctct atgagagaaa tacgccaaat
    36181 aaagaaatca tttatcctat ctgagaattc ctctaacaaa tagtctaata gctctcctga
    36241 ttgtatccgt tccaaaacat ctgtattgag atggtcataa tcttttaatt ttatctcaat
    36301 taaactaaat ccatcctcta cttttgaaag ctgctgacga atgtgaacaa tgacctcttt
    36361 tgtcgttctt gctttcctca atgatatttc ctttgtcttc caatagacct ctgctacttt
    36421 aatcggatgt atttggcaac ttcctttact taattcaact agtagaatag atgtagaagc
    36481 ttgttccttt ttagtatgac cttgaggcgc acctggataa acaattgttt gcttttcatt
    36541 taagaccgtt ggcacatgga tatgccctaa agcccaataa tcgtatccct tttcttgcat
    36601 ctttgagggt tggaaaggag catagtttcc tttttgagag acgccaggtt ccccgtggta
    36661 caacccaata tggtaatctg tgagttctct agaaggaaat tccatcactt tgtctttttg
    36721 aatccacggt tgacgataac taaatccact gagagagaca gtttctccac tttttatcgt
    36781 tatttttttt gtctctaccg tttcggatgt aaataaatgg acatttttag gaaaggagaa
    36841 ccaataccgc tctttttgat agaaatcatg atttccgaat attataaata caggtatatg
    36901 gttcttttct aaccgtttca tctgttctga aaaatgtttc tgtattttta atgaaggacg
    36961 attttgatga aaattatctc ctgcaagtaa aacaaaatca acgttattta ttattgcctg
    37021 ttcaatgata tttgataaaa cgg
//
LOCUS       DACDSY010000007        35375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDSY010000007 DACDSY010000000
VERSION     DACDSY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.007"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            366..686
                     /locus_tag="IWK44_000362"
     CDS             366..686
                     /locus_tag="IWK44_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP9548918.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            840..1745
                     /locus_tag="IWK44_000363"
     CDS             840..1745
                     /locus_tag="IWK44_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9548919.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1742..2515
                     /locus_tag="IWK44_000364"
     CDS             1742..2515
                     /locus_tag="IWK44_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9548920.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2696..4120)
                     /locus_tag="IWK44_000365"
     CDS             complement(2696..4120)
                     /locus_tag="IWK44_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9548921.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4331..4777
                     /gene="nrdI"
                     /locus_tag="IWK44_000366"
     CDS             4331..4777
                     /gene="nrdI"
                     /locus_tag="IWK44_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP9548922.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4842..6824)
                     /locus_tag="IWK44_000367"
     CDS             complement(4842..6824)
                     /locus_tag="IWK44_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9548923.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6838..7734)
                     /gene="murQ"
                     /locus_tag="IWK44_000368"
     CDS             complement(6838..7734)
                     /gene="murQ"
                     /locus_tag="IWK44_000368"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9548924.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7737..8798)
                     /locus_tag="IWK44_000369"
     CDS             complement(7737..8798)
                     /locus_tag="IWK44_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9548925.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(9009..9536)
                     /locus_tag="IWK44_000370"
     CDS             complement(9009..9536)
                     /locus_tag="IWK44_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9548926.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9603..11105)
                     /locus_tag="IWK44_000371"
     CDS             complement(9603..11105)
                     /locus_tag="IWK44_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9548927.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11363..12484)
                     /locus_tag="IWK44_000372"
     CDS             complement(11363..12484)
                     /locus_tag="IWK44_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9548928.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12610..13551
                     /locus_tag="IWK44_000373"
     CDS             12610..13551
                     /locus_tag="IWK44_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9548929.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13701..14597)
                     /locus_tag="IWK44_000374"
     CDS             complement(13701..14597)
                     /locus_tag="IWK44_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9548930.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14866..16206
                     /locus_tag="IWK44_000375"
     CDS             14866..16206
                     /locus_tag="IWK44_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9548931.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16424..16867
                     /locus_tag="IWK44_000376"
     CDS             16424..16867
                     /locus_tag="IWK44_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP9548932.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17371..18789)
                     /gene="nhaC"
                     /locus_tag="IWK44_000377"
     CDS             complement(17371..18789)
                     /gene="nhaC"
                     /locus_tag="IWK44_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9548933.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19192..20415)
                     /locus_tag="IWK44_000378"
     CDS             complement(19192..20415)
                     /locus_tag="IWK44_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP9548934.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20446..21285)
                     /locus_tag="IWK44_000379"
     CDS             complement(20446..21285)
                     /locus_tag="IWK44_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP9548935.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21302..21982)
                     /locus_tag="IWK44_000380"
     CDS             complement(21302..21982)
                     /locus_tag="IWK44_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9548936.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTIYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21984..23057)
                     /locus_tag="IWK44_000381"
     CDS             complement(21984..23057)
                     /locus_tag="IWK44_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9548937.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(23704..24375)
                     /locus_tag="IWK44_000382"
     CDS             complement(23704..24375)
                     /locus_tag="IWK44_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9548938.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24462..27140)
                     /gene="mgtA"
                     /locus_tag="IWK44_000383"
     CDS             complement(24462..27140)
                     /gene="mgtA"
                     /locus_tag="IWK44_000383"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP9548939.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            27799..28236
                     /gene="ndk"
                     /locus_tag="IWK44_000384"
     CDS             27799..28236
                     /gene="ndk"
                     /locus_tag="IWK44_000384"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP9548940.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28258..29124)
                     /locus_tag="IWK44_000385"
     CDS             complement(28258..29124)
                     /locus_tag="IWK44_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9548941.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29284..31377)
                     /locus_tag="IWK44_000386"
     CDS             complement(29284..31377)
                     /locus_tag="IWK44_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9548942.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32126..32749)
                     /locus_tag="IWK44_000387"
     CDS             complement(32126..32749)
                     /locus_tag="IWK44_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548943.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32908..34305)
                     /locus_tag="IWK44_000388"
     CDS             complement(32908..34305)
                     /locus_tag="IWK44_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9548944.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34337..35308)
                     /locus_tag="IWK44_000389"
     CDS             complement(34337..35308)
                     /locus_tag="IWK44_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9548945.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
BASE COUNT        10169 a         7415 c         6207 g        11584 t
ORIGIN      
        1 atttttttat cacaaaaatt atcttactta ttgtaaggta aagctttttt taatttattg
       61 cccgataata tatagaaata aggtctagtt actgtttttt ttaaatgatt aataaataac
      121 aacttctttc tatgaaattg caaagcattc atcttctttt ccgctaacga tatatccgtc
      181 agtgcttggt acactttact aaaaagtatg agattgccga tatctggata ggtgtgctat
      241 actacagaaa aagatataca atctgccacc gggtaggata aagaggatga tcattccatt
      301 aggtctttga agggatgatt ttcctctttt tgtgtatttt tttatagtta aggaggaatg
      361 taagtatggc atggatggaa ttaatcatag caggaatttt ggaagtattc tggtcaacga
      421 tgatgaaatg gagtgatggt ttttctaaaa tcaattattc tttttacaca gtgattggca
      481 tgattgctag tttttacttt ttgtcaaagg caatcaaaag tttgccgatg agtctctctt
      541 atcctatctg gacagggatt ggcgcagttg gttcggtcat aattggtgtg gtcttgtttc
      601 atgataagct gaatctttcc acttggttct ttgtggggat gctactcatc agtatcattg
      661 gcatcaagat tactagcggc cactagacac ttggaataag tactgatcag aaaaaataag
      721 cctttttatt taatattcaa tttttttatg aaaaaataag aaataagtcc aatgacctag
      781 cgacttctcc actcatcagg tgtatgcttg tactatctat agaaaaggag tgaatatttt
      841 tgaaaagtct ttcggtccaa gaattatcca agaactttgg tcacaaacaa gccctcagcg
      901 atgtcagttt tagcttatcc cctggcaaaa tcgttggatt agtaggtcct aatggcgctg
      961 gaaaaacaac gatcatgaaa gctattttag gactgatcca ttactcagat ggaacaatct
     1021 gtattggtga aaatgagatt tctccctcta gtcataaagg gttggaacaa gtaggagcat
     1081 tgatcgaata cccgggtatc tatccattct taacaggcta tgatcatctg aagttattct
     1141 ctgaaaccaa cgatgtatct gctatcgaca ctatcgtgaa tcagctcaag atggaaaagt
     1201 acattcacaa aaaagcgaaa tcctactcgt tgggaatgaa acaaaaattg ggaattgctc
     1261 ttgccttact gaaccatccg tctatcgtta ttttagatga gccgatgaac ggtttggatc
     1321 cacaagcaac aagagatgtg cgaaaaatca ttaccgactt ggctactcag ggcacctctt
     1381 tcttgatctc cagtcactta ctcagcgagc ttgaaaaaat ggtcgatgag ctgatcctga
     1441 tcaatcaagg aaagatcatc aaacaatgta caatggctga attgaaccaa acagcgcaag
     1501 atttcatcgt tatctctaca acaaatgatc tagctgctcg aacgatcttg acagaagctg
     1561 gatatacttt ggaagatcct gataaaatca agcttttaaa agaaaacgaa gaattattag
     1621 gaaccgtgat ccagctattg tctgcacaaa atatccatgt gacagacgtc cagcatctga
     1681 cgaatgattt ggaatcttct ctattggaaa ttctggattc agtaaagggg gaagaagaaa
     1741 aatgacattg cttgtaaaac aagaattatt taaattgatc aagaaaaagt caactgctgt
     1801 tttatctgtt ctattagttg ttttgctcat aggaacagca cttctagcca aaaaatacac
     1861 gacgatcatt gatccagttg aaatgactgc tcagcttttt agtgccacat cttggattgt
     1921 ctttatcatg attgcagctg caagcacgat catttcgatg gaagcacaat atggtacatt
     1981 gaaaaatctc ctttatcgaa agtattctcg aggagaaata ttagtaagca aatggatcac
     2041 attagttatt tattctgttt atttatacct tttagctatt attgtcactg tgttgatgaa
     2101 actgattctt ttcccttcga tttccttcac ggagaaggtt tctactggtc aaacactgat
     2161 ccaaagcttg ttcacttata ctttgggtag ttatatcggt ttatggctga ttttaagttt
     2221 agtgttgatg attgcttgct ttatcaatag ttccggcgca tccatctcag caggaatcgt
     2281 cttttatttt gcttcgtcaa tcatatctgg tatccttatt gcactcatcc aaaaatggga
     2341 gtggatcaaa tggaatccga tcagtatgtt gaatctgcaa aaccaaattg gaaacgaaga
     2401 gatcatgaag caattgactc atctttctac taatcaaatg ctattcggta atttagctta
     2461 catcgttttg ttcttagcat taggatatct cgtatttaag cgaaaaaatg tatgatgact
     2521 catcaaaaaa gaggctgtga aagagacgtt cagccttatt gccgattggc tgtctcttaa
     2581 acaccgttta ttctgaaaaa ggagctgtga caacatttgt gtcacagctc ctttcctagt
     2641 atgtttttga ataaacgaca ggctcacaac ctctctttat acttctttta tacttctata
     2701 cgtgaagcat cccagtggca aataaaacca ttgcaatcac tccgaccatc gtcacaccta
     2761 taagtaaaat cttatctaag tggatatgtt gtacaggctc gttttgctcc ttacgtgctt
     2821 tgaggtaaaa ataaatgcct ggcaagaaaa gaacaagaac aagcattact tcattaaagg
     2881 ctacgactag catagcccat acttgaaagg cacttgctaa aagccctact aaaaattgga
     2941 cccattcttt ccgctgataa gaaagtttca tttggtacaa tcctaagaaa agccacgtga
     3001 agaaaatagc tgctgtacca agtgtgtaag caaagttata agcactgtca gtgaataata
     3061 atgaaagtaa aaagacattc gtacataacg tcgtcaatac gatagcataa gttggtgccc
     3121 catttttgtt gagttttccc catgctttag gcaaaagtcc gtcttcagcc atgtttccca
     3181 ttgtttcaat cggtaagatc gtccatgaga gccaaacacc aactaaagaa atgatcaaac
     3241 cgccgttgat cacgactgct ccccatttac caaccatcat ttcaaacaca tagcccatag
     3301 caggctgcgc cttgatcgtt tgcagctgat tcatcgtcat tgtcccatat ggaagcatgg
     3361 aaaccaagac gtaaataata atcaagccga atatccctag aatcgttgct ttcccaacat
     3421 ctgattgttt ttttgctcgt gaacctacga cagaggctcc ttcaatacca acaaataccc
     3481 aaagcatcac catgatgcaa ctcaatactt gactggaaaa cgggatcggc tgtccattcg
     3541 cattcaatat aatgtcgcta gaaaagttat caatgaattg ttggatcgaa aacgtgctga
     3601 tagctactac aataaataaa aataatggca ctaatttaca gaatgtgacg atagcattga
     3661 cgaaactagc attttcgact ccccgattca ctagccatgc atatccccat aaaaagactg
     3721 tactcatcac gatcgatgcc agattttgcc caccttcaaa tactgggaag aaatatccta
     3781 atgcgctcat caacaatgtt gcaaaagcta cgttgcctaa ccagcatgtg agccagtaac
     3841 cccaaccgct gataaaacca gcgaaacggc caaaaccttc ttctgcgtaa ctaaagatcc
     3901 cgccttttaa atctggacgt tttttattga tattattgat cgacaaggag agcatcaaca
     3961 ttgctgttcc tacgagtacc caactgaaaa tagctgggcc gccagccgat ccggagatgt
     4021 ccgtcataat accaaatacg ccacctccaa tcgctgaacc aattactaag gcagcaagtg
     4081 gaattaaacc aattcctttt ttctgttgct ctgtatccat gtaagcatcc tccaaaataa
     4141 aaccgctttt atcaaaaagt ataacaaacc ttttttattt ttacttaatt aataaaaact
     4201 ttaatagaaa tataattcta ccggcttttt tctcattata caagcaaagc gcctagtccc
     4261 tttccaaatt acaccaccaa aaaacttaaa aaagatgcta tactaaaagg aaagaattgg
     4321 aggatgatcc atgaacattt tatatatatc gatttcagga aatacgcgtg cctttgccaa
     4381 acatctagca gaatatgctg aaaaaatgca tgcagaagat cctgtcaatc ctgaagttac
     4441 tttaaaagaa atccatgaaa atagtgattt tacgaaagaa acagaaccat tttttacttt
     4501 cgttcctact tatttagacg gcggaaatgg tttagataac ggtgatacag aaatcttgac
     4561 cgaaacgatg cgtgaatatc tggaacatga agacaatcat aaactttgtc ttggtgtagt
     4621 cggtagcgga aataaaaact tcaataatca gtattgtcta acagccaaac aatatgcaca
     4681 aacatttggc tttcctttct tggcagatta tgaattacgg ggaacaccat ctgatgtgac
     4741 acgaatttat cagattttag cagaaaacaa taaataataa aaaagaagaa gctgaaaaat
     4801 agatttcagt ttcttctttc gtttctattg tttctatcat attaagcttt gacatccatc
     4861 acaggtgctt ttgcttttgc tttacctgtt ttttccaaga caaagttttt tacacgatcc
     4921 atattggtta ccaagacgat catggatgtt tctttgcctg ctgctttgat cgcttctaag
     4981 tcagcttctg ctacaagcgt atcaggtgtc acttgctgcc cttctgttac tttcacatca
     5041 aatggcgtac cgcctaagtc aactgtgttg attcccatat gaagcaagat ttctaaacca
     5101 tttggcgttg tgatcccaat agcgtgtttc gttgggaaga cagagctgac taccccagta
     5161 actggagaat agatatgtcc gttttctggt tctacaaagt agccttcacc catcatcttt
     5221 tgtgcaaaaa ctggatcact tgctttatct aaagaaacaa ctgttccgtc agcaactgat
     5281 gagaggatag tcacactttc agctgaagtt tgaggtacta ctttttcagg cacttcattt
     5341 actgtggctg tttcagtcgc taattcttct ttttctaaac ggtctttagg aataccgaag
     5401 aagaatgtag caacgaagcc accagcataa gctgccaata atccaaggat gtagcctaac
     5461 catttaccgt cagagatcaa tgggatcaaa gcggctcctg aaggtccaat agcgatagcc
     5521 ccgacatttc cgatcattcc gatcactgcc ccgccgatac ctccaccgat acaagcagta
     5581 ataaatgggc gtcctagagg taatgttact ccgtaaatca atggttcgcc gattcctagg
     5641 atcccgactg gtaaagctcc tttgatcatt tctgttaatt ctttatcttt tttgcagcga
     5701 atccataatg ccaatgctgc acccacttgt cctgcaccag ccatcgcaag aattggaagc
     5761 aatagcgtac gaccagtttc atcaatcatt tgcacatgga ttggagtcaa aatttgatgc
     5821 agtccgaaca taaccatcgg caagaataat gtgcctagaa cgaagccggc aaatgctcca
     5881 ccaacgccaa gtacccagtt gatcccaccg accatgccat cagaaatgaa acctgctaaa
     5941 ggcatgatga agaaaatttc aatcagaccg atggcaatca atgaaagagt tggtgtaacg
     6001 atgatatcta ccgcatcagg tactacttta tgcagttttt tctcaacgat tgacaatagc
     6061 caaacagcga agatgacacc taggatccca ccttgtccag ctgcaagagc ttcaccagta
     6121 aataagtttt tcagtggtgc ttccggattc atccctgtca gcatcacaac ggccccgata
     6181 actccaccaa gagttggtgt tgcaccaaaa acttgtgcag cattgatacc tgtatagata
     6241 actaggtagc taaacatccc gtttttcaag atgttcatca catcgatata ttgttgccat
     6301 gttgctgcat caagtgttcc ggctgtgact aagtttgcta aaactgcggc aatccctgcc
     6361 acgatcccag taccgacaaa tgcaggaatc attggcacaa agatattaga aatgtctttt
     6421 aataccgatt tgattttaga tggtttttgt ttcgattttt gtgccgcttt catttcttgt
     6481 gcccgttcgt ttacacggtc ttttccagat gctgatgaat ttgctgtagc tgtacttaca
     6541 gaagaaagtt tttctcctaa tttcacgcct gccaaatcaa ccatttgctg tgctactttg
     6601 ttcactttcc caggtccgat gatgacttga agctgttcat cttctactac accaagcact
     6661 cctggaatcg cttttaattc agagacattt acaagatcat cattgatgat cttcatacgc
     6721 acacgagtca tgcagtggac aatgctttct acattgccca tgccgcctgc ccctttataa
     6781 atttgttttg ccagttcttc caaagtcagt tcgttttttg ccatggtctt ttccccctca
     6841 taaggtccct tttacaaatc cgtttgcttc agctaatttt gcttgagctt cctctttatc
     6901 actatttgtc aaaatcatga cgatagccag cttaacattt tgatctgctt cttcaaagta
     6961 cttttcagct gtttcgtaat cagcttctgt cgcttgcatg atgattcgtt ttgagcgttc
     7021 gattagtttt tcatttgttg gtttgacatc gaccatcaag ttattgaata ctttacctat
     7081 accaatcata ctgatagtag aaatcatatt caagataagt ttttgtgctg ttcctgactt
     7141 caatcgagta gagcctgtca aaaattctgg acctgcatcg acttcgattg ggaaatccgc
     7201 gtgtttactg atttcggctt gtttattgca agaaatcgtt cctgtgacag caccgatcga
     7261 ttttgcgtaa tctaatccac cgatcacata tggtgtacga ccgctagctg cgataccaat
     7321 aactatatca ttttttgtca aattcagctt gattagatct tcttgcccta gctcataaga
     7381 atcttcggcc ccttcgacag ctacagtcat tgcttcttct ccaccagcaa ttagacctat
     7441 gaccatttcc ggttctacac cgaatgtcgg tacgcattca gcagcatcta acacacctag
     7501 tcttccactt gttccggctc ccatataaat cagacgtcca ccttttttaa aggactcaat
     7561 cgtgttagca atcactggtt cgatacttgt cagttctttt tcaaccgcag cggccacttt
     7621 ctgatcttct ttgttcatta gttgaacggc ttgagaaata ctcatctcat ctaatccaaa
     7681 agtttcttgg tttctgcgtt ccgttgttag gttctctaaa ttgattttgc tcatttttat
     7741 cttccttctt ttcttataaa ttgatagttc ataccagctt tgatttggtt gatcaatggt
     7801 agctcttctt tgataacttt agctactaca ttcacttttt catccgctgg caaatcatat
     7861 ttggttaatt ggatctcacc catatagcgc aaatactttt cgttatctaa agtaatagac
     7921 cctttcatac gcgtagctgt atttcttgct gggataggcg gaatctctcg aaaacgtgca
     7981 tctacgcttc gtatgacatc tcttgcatcg tcttgccgat tcgtatgctt ccctaatata
     8041 tattcgtaaa attcttcatc tacaggttcc gtatggagca agactgtctg ttctttttgg
     8101 aaagaagcaa attgctcctg aacgttttct gacagacctg catctccgat gtaaacaata
     8161 tccgtgttac ttgctaatag atctagagcg cctgcaagag gatggatccc tcgatgttct
     8221 tccaaagttg gcaatccttg atatagtggg cctctcaact ctgtatctcc tggtacaaaa
     8281 ccttggacaa ggagaccttg aattttgagc cattgatttt ttttctgata ccattctttg
     8341 tccagccctg tttcgggacg tggataataa ttatgccaag cttcaatctg tgagaaatca
     8401 gcttctgctt ctttcaattc atcgatatct ttaggcgtga tcgtgctagc attcaaactg
     8461 attttcaatt gatgtgacca gtcggcaatc tgctgattgg aaatatgata atccattctt
     8521 aaacctgtta caccaattgc tttcaaaggc gttagatctt ctaatgaaaa acccgctcgt
     8581 tccaatgcct ctcctgaaat atcgaccatc aattctagtt tattcttttt cgcttcgctt
     8641 cctagttcga tcaatcgctt ttgatatgcc gaaacatcat cttctggtat atgcatggaa
     8701 gtgaaaatac cgacaaagcc attggatgcc atttggcgga tatactgttt cttctcttct
     8761 gacaaagaat cgttcataaa tattgaaaag ccaaacatat tatccctctt tttctgtatc
     8821 cgtcttcatt tctatgacta gagtatatat ctaaagaaac aaaatttcaa tacttttatt
     8881 aaaataatta aatttcattt ctaaaggttg agtttatatt atcaaaagaa ttgtcataac
     8941 ctaaaaaagg cactgctaag caaaagcagt gccttgaaat attttattta aataacacaa
     9001 aaacataatt actcataaga aaccaattct gtattatcta ccatgaatgt aaagaacccc
     9061 tcattaggca tcggatgaaa ataagcattg atcactctta tcaaggcatt atggcaaact
     9121 aacagataga cattttcttc gtcttcgatg cattctttca ataaaggaac gatccgtgca
     9181 tacacatcta agactgactc cccatttggg aatcgtacag cgaacgctaa acgtgctttt
     9241 tgaaagttct catctttgct tggcagtcca tcataatccc caaaatccat ttctaccaat
     9301 cgttcatcaa cggtcagcgg caagctgaga cgatcagcaa ctgcttgtgc tgtatctctt
     9361 gctcgttgaa gaggggaatg gatgattttc gtgattggaa caggcaaatc tactaccttt
     9421 tctgctagtt tctgtgcctg catctgtcct ttttcagtca gcggcacatc tgcccgtccg
     9481 caaactaatc cttgtgcgtt ccaagttgtt tctccgtgtc tcgttacata aagcatgtga
     9541 ttctctcctt cgttggcaaa gttcccctta accatacctt gttgttcttg agattacaag
     9601 ggttagattc tctcaagaga aataatctca gtagatactt tatccaggaa ggtttgatca
     9661 tgttccacta gtaacatggc aggtttcacg ttcaagatca gttgttctaa ttgttcttga
     9721 ttgaagacat ccaaataatt caatggttca tcccatgtat atagttcagc tggctgtgat
     9781 aaagatttag ccaattccac ttttttccgt tggcccatac tcatctgctc gatcttgtta
     9841 tgaaaaacat ctctttccat cccaagcttt cggaggttgt tcaaaaatgc ttggtagtct
     9901 acttggtttt tctctgcaaa ttccgccaac gttcctcgat tgtcttcata attttggctt
     9961 gcataactaa tgctcagatg ttttggctgg tatttttctc ctataacctt gccgttgaat
    10021 gcccccagaa gataatggat aatggatgac tttcctgcac cgttaggacc tgaaatcgcc
    10081 acccgctgat gaggctcgat tgtaaaatga attggctcga ataacagatt ttcataccct
    10141 aattgaagat cttctacgct taaaagtcgc ttatggtgag acgcttggct attcatcgtc
    10201 aaagaatcga tatattcgat atcttttaat agtttttctt tctcgctgat ctgggtctcc
    10261 atccgattca cgattgcttt ggatcgtttc atcgtccgtg cagcatcagc acccactgct
    10321 cctttattca ctcgtctaga ttcagtatcg atgaatccga cttgtttctt tgttttatct
    10381 ccttctcggg aacgagacca ttcggctttt tcagctgctg ttttctttag cctactgact
    10441 tcttttttca atttttcatt ttgagccatt tcaaattcat cacgaagttt tttctgttct
    10501 tcatagatag agaaattccc ttgataaagt tccagttgac ttttttcgat tgctaaaaca
    10561 tggtccacta cttcatcgat aaatccccgg tcatggctga ccacgatgaa gccttgtttt
    10621 ttctttttca aataagccgc tacttgtttt ctaccagaga tatccaaatg attcgttggt
    10681 tcatcgatta acgggaaatg agtgtcatcc acaaataaaa gtgccagtag gacttttgtc
    10741 ttctctcccc ccgatagtgt gctgaattct ctccataaga tttcaggatc tgtttgcatc
    10801 aattggagct ctctttcgat ttcccatatc tcaaaatccg taatatcatt taacacgtaa
    10861 taggttaaac gttctttatc ttttgtctgt tgcgggaaat aggcaaattc ttgctgatgg
    10921 attacttgcc cttgataagg tagtttgttt tgtagaatat tcagtaaagt tgtctttcct
    10981 cgaccgtttc gtccgataag tcctagtttc cattgtgtgt caaaatttag atttgcttgt
    11041 tcaaataata atgtgccttg gctgtcgtag ccgaatgtca gattttttat ttcgatttta
    11101 gacattttcc atcacctcgt aaaaaaagac cagtcctatt ttcagaactg gctaaagagg
    11161 atgcaagggt tggatacaca caaaaagacc catacatcga aaaaaccaag ccaatcccga
    11221 aaatctgcac actgcttcct tgtctccaca aggtacagtc tgaatgcagc tttcatcaat
    11281 cgacttaatt tttcaatgca cggatccctc actttgtcct atatttgaaa ttacgatagc
    11341 ataaagaaaa tgattttgtc aactacttcg ggaggatggt gaaaaatctg ctaagtgtct
    11401 gtcctaagat ttttgttttt tctaatgttt gcctgtgttc cattgcttct tctaaaccta
    11461 ttggacccaa atgaatactt aagacaattg ggagaaaatc agaaagtcct tctagctctt
    11521 ggtctctact tccgcaaata gcgattattg gttttcgata tttttttgca agcactgcta
    11581 caccgtatgg aacttttccg tggatcgttt gctgatcgat ccgcccttct cctgtaatga
    11641 tgacatctgc ttgttcgatc tttttttcca atccagtgat ttggctgact aaatcgaaac
    11701 cagaaaccat cttcgctccc aacaaaaaaa gggctccacc aataccgcct gctgctccac
    11761 ttcctggaat atcatctaaa tccacctccc agaaatcttt tatttgctga gcaatctttc
    11821 ctgcttgttt atctaagaaa gctagcgtcg tatctattcc tcctttttgt cttccaaatt
    11881 gccaagcagc tccttgttga cctgtatatg gattggtaac atctgcagcg ataatcaatt
    11941 ttactttaga aacaagaacg tttcttcgat caaaatgaag ttttttagta gataataaag
    12001 gatttccttc tgtgtaccct tctaaagaag cacccaagga ttggagtaat cctagaccac
    12061 catcgctaca cccgcttcct cctaaagtga caataatttg ttctacttct tgaagcagtg
    12121 catctttaat aagttctcct aaggcataac tggaagcaag tcggatagtt tgatccgttg
    12181 gtttatcaag tagatctaac cccaatgctt gtgcagattc gatgactgcg gtgttttttc
    12241 cttgccaagt tgtcaataaa tagctgattt ttcgctgacg gaataagaga tccatcccat
    12301 caatggtacg ccatgttccc ctgagcgcgt aatagattgc ttctgcagta ttttctccgc
    12361 catctgcaat cggcacattg atttttcttc ctttacgatc ttcccagcca tttagaactg
    12421 cctgatttag ttcaaatgaa gaagcacttc ctttaaatga atcaattgcc gccaaaatat
    12481 ccatctgtat ccctcacttt cttcttaagt aagacacttt ttttcatttt tttcaaacaa
    12541 aacaatgcta ttttcttcca tttttctata tactaaaagg gaactcattg aataaaggag
    12601 agaaaaaaca tgaaaatcgc aatagctggg gccggagcaa tgggcagccg aatcggattg
    12661 atgctacatc aaagcggaaa tgaggtcctc ttgatcgatc gttggcctgc tcacattgaa
    12721 gccattcgta caaatgggtt gattgctgac tttaacggta aagaagtggt cgcaaaactg
    12781 ccaatctatt caccagaaga aatcatcgaa agcaacgaac acgtggactt gatcgtagca
    12841 ttaacaaaag ccaatcaatt agatgatatg ttttgttcga tccaatccat tattactgac
    12901 aatacttatg ttttgtgttt actaaatgga ttaggtcatg aagatgtact ggaaaaatat
    12961 gtgccaaaga aaaatatctt atttgggatc acgatgtgga cagctggtct agctggtccc
    13021 ggaaaagtca cattgttagg tgatggagaa atcgaactag agaacttgga accagaaggc
    13081 gaagcattta caaaaaaagt tgtagaagtc tttcaagaag caaatttaaa tccgatctat
    13141 agccataacg ttcgttattc catttggcga aaagcttgtg taaatggaac attgaatggt
    13201 ttatgcacga ttttggattg taatattgct gaactaggcg cgcaaaaagc ggcagagagc
    13261 atggttcgaa caatcgtatc tgaatttgct tctatcgcag caaaagaagg aatcatacta
    13321 gatcaagaag aagtgtacca acatattgct tctacttatg atcctgacaa catcgggttg
    13381 cattacccat ctatgtacca agacctgatc aaaaaccatc gtttgactga aatcgactac
    13441 atcaatggtg ccatttggcg aaaaggacaa aaatatgata ttgctacacc atattgtgcc
    13501 tttttgaccc aattagtcca tgcaaaagaa ggaatccttg gcgcagaata aaaggctctc
    13561 tgatgagaat cagctgaatc agcagctggt tctctttttt taagcccaat aaaagatcaa
    13621 aaagaaaaca caactctcat gatgccataa gaattgtgct tgtttgtttc agtacataat
    13681 ctcttcaaaa tgtaggtttt ttatttgttt tcaacagagg agaggtcata ttcgtattgc
    13741 tcataaatag tggaatcgtt agtgataaat cgttttgccc aattttctgc atcataaaat
    13801 tccaatagct ctgataggat tggcttaaat ggtgtatgca gaaattccgg taattcatct
    13861 aaatcacgtt ctcgaagcaa aaaaatattt tcaggacgat aaaaatgata atgagcaatc
    13921 gcttgattat ccgtcactag tttcttttga aaatacatgg attcaaatgt ccgcaaagtc
    13981 actccttgct gaccctcccg caacagttcc agaatcccgc gcgattcccc tgtttttttc
    14041 aggtactctt cataagtaag atatccaggt tgttcattcc ctcgatgtgg aagaatgaaa
    14101 tagttggtcg taacatctct ttttataaat tcctgcttat agctttctgc tcgttctttt
    14161 ctcccattat tcgttgcacc aaaaaataga tccgtctttg gttcgctaga agaaaaattc
    14221 atatgtttcg aataaaatga actattatgt ttcagcccaa gttcttttgc ttccaaaaaa
    14281 tcaaaatgat aaatctcatc gattgccggt tcatcttgtg cctgcttcag catcgcctcg
    14341 tgttctgctt tgaagtgatt ccaaaaaaat aacacaattt ttgcattggt aaatgggcgc
    14401 acgcgtcgag ccatctccat atcaaatcca taatctaaaa aaacgatcag ctgataattt
    14461 gccaactgtt gtctagctga tttaatgaat tccgactgta gtaaatcttt tccggaccaa
    14521 acatctaaag agcggatgga aggcaaaaaa taaagtgatt tattttttgc aactagcagt
    14581 acgtcatttt tgttcatctt ttccgcctca ctttctctgt cactttttcc tatgatagcc
    14641 tgtttaaata gtaaaaagcc agtaaaacgt ttcctatatt tgtatgaaaa tttaacattt
    14701 gtatgaaaat atgatgaaat aaatcattct tctattaaaa ttcgcctaaa agttttctga
    14761 atagttgtaa ttgtctgaaa atttgttatc attagtcatt gttaaatatt gctatttatt
    14821 cttaatataa attttgattg tgaatttaaa agaaagaggt catttatgga agataaaaaa
    14881 ttgaaaaaac aacttagctc cagacatatc accatgttag ctttaggtgg agcaatcggt
    14941 gcaggtctat ttaaaggaag tggcgaagcg attgggatcg ccggcccttc tgtattgatt
    15001 gcttttttga ttggtgggct gattttatat atcgtgatga aaggtctggg aaaaatcgtt
    15061 ttaagcggtg gcgacacgca tcacggatta tctggtttga tacgccctta tctaggtgct
    15121 cattctgctg attttactga ttgggtctac tggtctatgt ggattatcaa tatcatcgct
    15181 gaagctgttg cagctgcttc ctttttgcag ttgtggttcc cgcacattcc agcctgggta
    15241 tttgttttta tgttggctgt tttaactacg atcatcaatc tttattcggt acggttattc
    15301 gcagaaacag aatattggct ggcttttgct aaaatcagtg tcattatctt actgatcatt
    15361 tttgctactt atcttgtcgg tcagcaaatg ttgggcacag gcgttttccc tacccttcag
    15421 caattgactg accatggtgg gtttactcct catggtatga agggaatcat cagttctttg
    15481 cttgtagtta tctattctta cggcggttct gaactgattg ctatcacggt aagcgaagca
    15541 gatgatccta aacgtgcgat tcctaaagca atcaaaggtg tcatgggaag aatcatttcc
    15601 ttttacatca ttcctttgtt tttgcttttg attctttttc cttggaatac tttagcaggc
    15661 acgaatgtca gtccttttgt gatggtattt gaaaaaatga atatcccatt tgcagcggat
    15721 atcgtcaatt ttgtgattgt ccttgctttg ttctcatcta tcaattcggg tgtgtatgca
    15781 tcttcaagaa ttttgttctt ccgtttgaag gatcgaaaag gttcctctag aaaactagct
    15841 gttttaaaca agcatcaagt accgcaacgt gctgttttat tttgtaccag tgttctttat
    15901 cttggtgtag ctctttctta ttttgtcggt gacaaattat ttggctatct ggcaggttcc
    15961 ctttcgtata cagtattatt gatctggatc attatcagtg cggcatcctt tgtactggca
    16021 ctaagaaaag gaacattatg ggataaagga atcagctttt ttgcattagc tgtcttagga
    16081 ttgattttcc ttggtatcct gttcacgaac tccattgggg taactgtatt gactggcctg
    16141 ctttacctgt tcatttattt cagttatcaa aaaaagaatg atgcgttcgt attgacgaat
    16201 gaatgaaatc gatagaaaat tttattttat ttatgtggaa tagatggagc tattgaagaa
    16261 aggagttgtg acaaaagtct tgttgcaact cctttttccg aataaacagt ctcatgacct
    16321 cttggtaaac ggtgttcaaa aaccctctgt gcaaaataat tgattgttct ccatgctcat
    16381 gacatgatag aagaaaggaa tgaaaaaaag gagtttgttt taaatgaaaa agtattggaa
    16441 gtttttgttg atgatcggtg tttcaacggt agttatgttt ggtatgatgt acttgaatgt
    16501 ctatgcgctc gatcatatct tcttcagtcg tacacgagta ttcatggcgt tgatgatggg
    16561 tgcagtcatg gcagtcatca tgctgttgtt catgtggaaa atgtatgaaa acaaaaagat
    16621 aaatgttatg atcttagccg ctagtcttct tgtttttgcc ggttccttgt ttatggtacg
    16681 aagccaaact actgtcaacg acacagcttg gatgaaggcc atgattcctc atcattctat
    16741 cgctatcctg gcaagtaaac gagctgattt aaaagatcct gaagtaaaaa aattagcgga
    16801 agaaatcatt caggcacaag aagaagagat cgacaaaatg aaaaaaatga tcaaagaaat
    16861 ggaatgacca ttatccgtcc aaataaaaaa agaggatgtt ccagaagtta tcagcttcaa
    16921 gaaataagaa gaagctactt ctggaacgcc gtgaataagg ggttgtgaaa aagctgccct
    16981 gcatcaagta ataagaacag ccaaacaaaa atagcttctc ctatttttcg tttggttagg
    17041 cttattaccg cagatgcaag cttttgaaca ccgtttatta agaggttgta aaatcagcgt
    17101 tctgacctga gtattagaga gacaaatgga aaaatagttc ctcatatttt gccatttttt
    17161 gagctaatac cgagggtcag ctgattgaac accgtttatc gagaggttgt gaaaaagtcg
    17221 cttagtttcg cgtattaaga aaaattttgt gaaatggctt tccacatttt tgtataaatt
    17281 tggcttaata ccgagaaact ggcttttgaa taccgtttat tcgtaaaagg gattgtgaca
    17341 aaagtcttgt cacaatccct ttttatgttt ttattgttct aattgcgctg tcttgcttac
    17401 tgcttcttgc ggtttcacaa atttttttga gattccccaa cctgtgaagg cactgaccat
    17461 cgaaaacaca ggagaaagca aactgaaaaa tacgaatggc aagtaagtga gtgtaggaat
    17521 accaaatgta cttgctacga aacttccggc aattccccaa ggcactaagt agttgattac
    17581 tgttccacca tcttctagcg ttctacctaa tacagttgga tcaaatcctg ctttttcata
    17641 agtttcttta aatgcattac caggcagaat aacagatagg aattgttcac caataaaaat
    17701 atttactccg atacttgtca tcaatgttgt aaaaatcaaa tttggtgtgt tcgtcatctt
    17761 tcttgatact cgttccatca cggttgagat caagccaaat tcgatcaaca atccacctaa
    17821 tgacaaagtt agaataatca atgacacagt aggcatcata ctttcgattc ctccgcggct
    17881 aagcagtgaa tcgatttgtt ggtttccagt atttgaaaca aaccctgatt cgatgacccc
    17941 agccaacttc gttaaggaag tttgtgggtt ttgaataaaa atcattccta ctgctacagc
    18001 gatattcaac aaaatcgtta aaattgccgg cacctttttc catgcacaca gtaacattaa
    18061 aaataaagga atcagtgacc agattgaaat agtaaagttt gcttctagaa catcagtcac
    18121 ttcacgaata gatgaaagac ttgcggcttt acttgtgtgt cctaaaatca tgaataaaaa
    18181 gaaagaaaca acaaatgccg ggacagttga ccacatcata tgcttgatgt gtttgaacaa
    18241 atcggcatca acaatagctg ctgctagatt tgttgattcg gataatggag acatcttatc
    18301 tccaaaaacg gcacctgaaa caatagctcc aacaaccagc gctggattta agcctagtgt
    18361 tgtcccaata ccgaaaaatg caatcccaat cgttgacata acagtaaagg cactccctac
    18421 agcgctacct acgattgcac acacaacaaa tacagaaggg acaaaccact ggacactgat
    18481 caatttgaac ccaatgacca ttagagttgg aatgatccct gcttgtatcc atacagcaat
    18541 caatgaaccg actaaaataa aaataaagat tgggatgatt cctggtttga tgccattgat
    18601 gattccttcg tgcagccaat ctgtcgggta tctttttatc actgcataca acatgaccat
    18661 tccgatcgct gctaagatcg tgatattagg tgagaccttt aatccgacaa cgccaatcgc
    18721 aatgcctaaa acaataacca ttaatacaag tgttgcttct aaaaaacttg gtgttccttt
    18781 ttttcccatg ataatctctc cttttttagt tgttcttatg ttctaaaaaa gttaagatat
    18841 cggattactc cgccgcaatt catccgcgat tcctccttta taaaaataaa aagacctgtt
    18901 ttaaactaag gacgaatgac tccgcggtac caccttaatt aaaaacagat ctgtttttca
    18961 ctttcaaact tttattcaaa tgtatggact ttccatgtaa ttcgtcaatt aggcttgatc
    19021 ggttcacagc gaccaccgac tttctgaaca aaacacctaa ccgctactgc gtgaaaagtt
    19081 gacttgtacg aagtttacct tccataagat catttgtcaa tgaaaaagaa aatatttttt
    19141 catttttctg aaaattattt ttataacttt cacacacaag tttatttttt actagaaagt
    19201 agcgccctct tgtttttttc ttgctactat ttcttgatgt tcttgcttga ttctttctaa
    19261 tagctttggc tctttcaaca gatcaagagc tgtattggcg agtagttctg ctgcaatcgc
    19321 aatagaagca aggcctttgt cacttttagc agcagctttg aattcttctg tgtgtccggc
    19381 aatatatgtc tcagagattg ctaccgtagg ctggattgtt ggtatgactt ggctgacatt
    19441 tcctacatca gaagaaccga gacttgttct ttgttccgtt tctatttctt catctggaac
    19501 acctgcttct tttgcatgct ggaagaataa gtcatcaaaa gaaggagtaa cgatgacatc
    19561 atcaactgca ttttggaaca atccaaactc aaaagtcgtg cccgtactta tcgcagcacc
    19621 tcgtacgata ttttccactt tttcatatac tttatctaat gtttgccgat ttcctgcacg
    19681 taaatagaaa cgtccagcag cgtaatctgg aatgacgttg gcagctactc ctccgtctgt
    19741 aatgatccca tggatattga catcttttgg caattgcaag cgtagagcgt tgattccatt
    19801 aaacacttgg atcaatgctt ccaaagcatt tactcctttt tcaggtgctg ctgctgcatg
    19861 ggaagcgaca ccgtaaaatt tgatgtcgac tggatcattc gctaaagatt ctgtcgtttt
    19921 cccataacga aaagctggat ggacgcataa cgctacatcg acatcctcaa aaaaaccttc
    19981 tcgaacaaag ctgcctttag ctgatccgtt ctcgccccct tcttcccctg gggtgccata
    20041 tacacggatt tctccaccag tttcatcaat gaattgcttc actatgctgg cggctaatac
    20101 ggagtaagta ccaaacaaat tatgcccgca tgcatgacca atacctggta aagcatcgaa
    20161 ttctgccaaa aaggcaattg tgggaccggg tttattggaa atataacgtg catcaaaggc
    20221 tgttcggtgt ccagctactt ctttttctac ccaaaacctt tctttttcta attgtttaat
    20281 caacgctgct gaggaataaa actcatagtt acttacttca ggatgttcgt ggatatctag
    20341 agcaagtgct tgatattctg gtaaacgata ttggattaga tccttcaatt ggtttgttga
    20401 gattgttgtt gtcataaaaa attcctccta tgctagtcat ttttcttatt ttctcccgaa
    20461 tgtttcccat gcaggcacgc ttgatccttt tgatgttttc tcgattactt tgactgtgtc
    20521 ttctgtttga taagcgtcta ctattttttg atacgtttta ttgttttcat cttcttttct
    20581 tgcaacgatg atattcacat aaggacgtgc atggtcatcc actggttcta agaagatggc
    20641 atcagaagca ggattaaatc cagcatctac agctacaccg ctattgatca ctgaaatgtc
    20701 tacatctgac aaagcacgtg ctgtttggga agcatctaat tcagaaatgt ctagattttt
    20761 tctattctcg gtaatatcag aaacagtggg cgcttgtcct tttgccttat ccactttgat
    20821 caatccagca gtttgcaata acagaagagc tctgccgcca tttgtaacat cattagggat
    20881 tgctactttt gctccgtctt tgatatcagc tacatccttc aaagtgttag aataaattcc
    20941 cagcggtgca ttgacagtat ttccgatcga tactaaatca gttccgtgtt ctttattgta
    21001 attgtctaaa aagaattgat gttggaaaga attcaaatca atcgatccat ctgctaaagc
    21061 cacatttggt tgattgtaat ctgtaaattt cacgtattcc aaattaatgt tctccttttt
    21121 caaacggtct ttcacgttgt cccatacatc tgtatcttct ccaataatcc ctagttttac
    21181 gatagtttct tttttgtcag ttgtatctgc ttttccttgg ctacaccctg ccaaccaaat
    21241 aattgctaca aatgtaatga ctacaaaacg aataattttt ttcatttttt ctcctccact
    21301 ttcaatgtgt aattttttta atcagttgat tactgataaa ttgacttaaa aaaactaaga
    21361 ttagaatcaa taatgtagcc acaatcgtta catctgtttg gaatcgatta taccctctcg
    21421 atatagcaag attccctaat ccgcctgcac ctactgctcc agccatggta gtcagtccga
    21481 tcacgttgat gatagtcaac gccgagaccc gaatgatacc aggcaaacct tctaacaaat
    21541 acacacgtaa aatgataccg attggactgg ttcccatcgc ttctgccgct tcgatcaccc
    21601 caggatcaac ttctagcagt gcgttctcga tttgacgagc aaaaaacgga acaactccaa
    21661 aaatcaatgg cactaaagct gctttcgcac caatcgtcgt tccaactaag aaacgagtag
    21721 tcaatgctaa aagagaaagc aagataatga aagggatgga acgaatgata ttgatcagct
    21781 tatcaagtat ttcaaatagc agacggttag ctagtaatcc attagggcgt gtcactacta
    21841 acgtcacacc taagatgatt ccaatcatcc cgccaatgaa tgctgtccaa aaagacatat
    21901 atattgtttg cttcgtcgct tcgacaaatt catccggaat caaataaaca ttgggcaaat
    21961 atgttttaaa gaaatcagtc attttatctt cctccttcga tcactttgat acgactatct
    22021 aaatcgtttt tttgtaaaat atttattttt acaccagatt tgatcaaata atccaatgct
    22081 tttttccgct gtttggtttc tccagctaaa gtaacgatca aactccctaa cggcgtttct
    22141 tgtaataact ctacattgcc atataaaata ttcgctgtta cttgatattt gctgtacaga
    22201 tgagcgatta gaggttcgtt cgtctgattt ccaatataac ttaattccac cagccattta
    22261 ttggcgatct gatcggcaaa agcagaatgg gcaatgatcg tttctaatgc ttggtcaaga
    22321 tgagtagctg ttcgaataaa atctttggtc aattcttctt caggattgct gaagatctgg
    22381 atgctgctac ctttttcaac gattcttcca tcttccataa ccgctacctt gttgcagatt
    22441 tctttgacaa cttgcatctc atgagtgatc aggacgattg ttaaacctaa ctggcgattc
    22501 aattttttta acaatgcaag gatctgaagg gtcgttttcg gatcaagtgc actggttgct
    22561 tcatcacata ataatacctt aggttcattc gccaaagctc gtgcgattgc tactcgttgt
    22621 ttttgtcccc ccgacagttg tttaggataa gcagatgctt tttcttccaa accgacaagt
    22681 tccagtagtt cattcacttt ttgacgacgt tcctgtttcg attttccaga atactttaaa
    22741 gaaaaatcaa cgttgtcaaa aatagttcgg ctttccatta gattgaaatg ctgaaagatc
    22801 ataccgatgg cctttctttc ttttcgcaat tctttgggag agagctgact cagattcgtc
    22861 tgattgacaa tgacttctcc tgctgttggt ttctgcagca aattcatcac acgaactaac
    22921 gtacttttcc ctgcacctga gtaaccgacg attccataaa catcgccttt ttcaatcgtt
    22981 aaatctacag aatctactgc ctgtatctct ttttcttttt gtttgaaaca tacagaaaca
    23041 tctcgcaatt caatcattaa aattcctcct tatctaaaaa aagcgtcctc aagaaaatga
    23101 aaatgtcatt ttcttgagga cgcttttttg cgtgttacca cctcatgttc aagctgtgtt
    23161 cacacacaac tcctcttcaa gtacgatcat actctagcaa gatatcggtc gcagccgtca
    23221 tcgccttaca tctatgctcg ggatgaattg ctcgatggcc attttccaat agtcgttcct
    23281 tgcttcttct cagctaccga agctctctgt caaaggttct tctatcgtac ttaccatttc
    23341 aatgcaaata atattcaaaa aaaaagtcct tttactaaca taaactatgt cagtaaaagg
    23401 acgacgtatc gcggtaccac cttttttcgg aagtttcctt ccctcaagcc caacatctca
    23461 aaaagagaga ttggcgttcg ttttaacggt cgaacagttc cgaatttgcc tacttatcga
    23521 caaattattc tccaaggcca ttttcagctt ttcttacttg actcttctca ccagccagag
    23581 cctctctgca aagtttccaa acttactttc cttttcaacg aaatcgatat tttgttgttg
    23641 ggattaatct acatgaggaa aactgaaaag tcaagtacga aattcaaatt attttttcta
    23701 tatttatttt tctgtttttt tatttaacat ttcaataaca gttcgaagat cttttccttc
    23761 cgcaagaagt ttcaattttt ccttttcttc gacggtataa tttcggacaa aactggaatc
    23821 aggtatcaaa acaacgccta atcctgctcg tgaagcagct gttgctccta ctaaagaatc
    23881 ttccactgca aaggctgaac tagcttctaa tcctgttcgt tccaaagctg tcagataagg
    23941 aatcggtgaa ggcttgttct ccgacacttc gtctccgaat gtaaacgtgt ccaagtaatc
    24001 agctaattta aagtgatcca agatatctgt tgctcttttc gtaacagtgg tcgtagcaat
    24061 gcctagtttc atatgacgtt cttttaagtc tgccaatatt tcttttgcat atggttttaa
    24121 gtggatggct ccgcttgtca gctgttgata aaaatattct tcacgcttcg ctcggacttc
    24181 ctgtactttt tcagggctac ctgctgcttt cactaaaata tcaaaacttt gtttccagtt
    24241 ttttccgccc caagcgtcaa cgatttcttt tgggatatgg atatcaaatt gctgaaatcc
    24301 ccaaagccag ccatcacgat acgttttttc tgtgtcaact aacaaaccat ctaaatcaaa
    24361 tattgcacct ttcatctata cattaatcct ttccaaaaca atacacatag tttagcacga
    24421 ctggtccaaa aagaacagaa gcaaagaacc agccttctcg tttataagag ttcttggaat
    24481 cgacggatat agattttctt cacagctgtc acaagcgcca gataagcgat gactgtagca
    24541 aataaccata accagtaatt gccaggaagt ggcatcaagc ctaattcagc accaaaactt
    24601 gtaaaaggaa ggacagaacc gacaataatt cctaatgtag tgataacaaa catggcaaat
    24661 gttgcgttgc tttggataaa tggtactttc ggtgtacgca gtgcatggag cactaaggtt
    24721 tgtgtccata gtgattcgac aaaccatcct gcatggaata atgcgatgaa aatcactcgc
    24781 tgtgaactat ccaatgtatg gaaacttccg cccactacag caggacaaat aacaaaatac
    24841 atcaacaagt aagtagtaat atcaaatatc gaacttgttg gaccaaagta aaccatgaat
    24901 tccccaatac tagatgcatt ccattttttt ggctcgtcca aatattcttt atccatccga
    24961 tcccatggca aggaaatgca ggaaacatca tagatcagat tcaaaaacaa aagttgtaaa
    25021 ggcagcattg gtaaaaatgg caggaacgta cttgcaatca gcacggaaaa catattgcca
    25081 aaattagaac ttgctgtagc cttgatatat ttcatgatat tcccaaaagt ttctcgtcca
    25141 gaaagaaggc ctctttccaa aatcgtcaaa tctttttcta aaaggatcac gtctgcagat
    25201 tctttcgcaa tatccacagc tgtatctaca gaaataccga tatcagccac tttcattgca
    25261 ggagcgtcat tgataccatc tcctaaaaat ccgacagtat gaccattttt acgtaaagct
    25321 gtcgtcaaac gtgctttttg ctgaggattc aattttacga atacttcatg gtcttcagct
    25381 acttgtgcta attcatgatc actcatttcc gagattttct caccggtaac caattcattg
    25441 atctctagac ctacttgacg gcacactgaa cgtgtaacta attcgttgtc cccagtcaga
    25501 acttttacag ctacaccatg ttctttcaga gctttcaacg cttgtttcgt tgtttctttt
    25561 ggtggatcaa ggaaagcaag ataaccaatc aaaaccatgt ctgactcatc tttgactgaa
    25621 aattctccaa cgacgcttgg attggtcttt tgtgcgacac caatcactcg taagccatct
    25681 tcattaagtg actgtacttt tgcaagtacg gcttcttttc gttcttttgt taatggtgta
    25741 acagtaccgc caagatcaat aaatttagaa atgttcagca tttcttcaat cgcgcctttt
    25801 gtgatcattt gtgttttacc agctggatca gcaaccacaa cactcatacg acgacgttca
    25861 aaatcaaaag gaatttcatc cactttttga taattgatct tatctgtatc taaagtttct
    25921 gttgcttcat caatgatagc cacatctaac aaatttttca gccctgtttg atagtagcta
    25981 tttagatacg catggcgcaa aacacgatca tcttcatttc cttcacaatc catatgatat
    26041 tcaagaatga ttttatcttg tgtcaatgtc cccgttttat ccgtacataa cacatcgatt
    26101 gcaccaaagt tctggatggc attcaaattt ttgatcactg ttcctttttt ggccatcaca
    26161 cttgctcctt tgaccaaatt cgtcgtaacg atcattggaa gcatttcagg tgttagacca
    26221 actgctacag atagtccaaa aagaaaagct tctagccaat ccccttttgt aagcccattg
    26281 atgacgataa ctgctggagc catcaatgac ataaatttga tcagtagcat ggatgtttta
    26341 tggatgccta attcaaagct tgattgaatt ggtttttcag atagcgtttc tgctacatga
    26401 ccaaatagtg tttcatttcc aacagaaaca atgattcctt ccgcacttcc actgatgaca
    26461 ttacttccca taaagacaag attttcgtaa cttgtttcgc tttgctgatc gtcttcacat
    26521 actttcgcat gcttttctac tggatagctt tcccctgtca atgctgcttg ggaaatgaat
    26581 aaatcctttg cggacaataa acgcatatcc gctggtatca tatcccctgc tgaaagccga
    26641 acaagatctc cacagacgat ttcttccata ggaatctctg tgtactttcc attacgtttt
    26701 acagctgccg ttactttcac aagtgatttc agtttttcag ccgcttgatt ggaacgtacg
    26761 gactgaatca acgtcattgt cccgctgacg ataaccatag tgaagataat ggctacaccg
    26821 aaaagatctt tatctccagg agctgctaat acatattccg tgatgaatga aataattcct
    26881 aatgcgatca acactaatgt aaatggcgtg atgtatgctt tcaatatttc tacaatgaat
    26941 ggtgtttttt tgccatatga aatcgcattt tcaccgtaat tttctcttga acgttcgatt
    27001 tgttcttctg tcaatccatt tgctgatgca tggaatagtt caaataattc ttgtttcgtt
    27061 tttccagcaa aagctgcata gattttttgt gtatctttct ttttttccat ctcaatctct
    27121 cctttgtgtc caaaagacag ctgttctcgc tgcttttggt ctttagctat tcatcggatc
    27181 gatagagaaa aaactgaaag gggctgagaa cagatagata atagccctgg cctttcctct
    27241 agatccttgt ttttcgaatg actgtcatcc atttgttctc atctccttta ctaagtaatt
    27301 ttaattcatg acttccaatc atttttcgaa tagttatttt tccaattaga ccacctcctt
    27361 tttatttttc accgatgggg atttgcggca acaaaaaaac ctctcgccaa atactcagcg
    27421 aaaggtttga aaaagcatat taaaaaaagc ttctcagatc tcatcgttga gttttgacac
    27481 tgtacaacgt aaaaagcaga tacctgcttt tagctacctt atgaaaagcc ttatctcgac
    27541 aattcctgtt ctacccattg gcgtctctcg acgtttttgg gcagtagcct atgtttgtat
    27601 aggagcctca cctaacaaat cttattcggt tttccttcgt taatataaca actaaaaaag
    27661 aggatgtcaa atcaattttc catgtttttc atactttttt taaaatacac acgttatatt
    27721 cctgtctcac gcggttttat ttgtctcatt ttccacctcc cgctataata gaaatatagt
    27781 aggaaacgag gtgctgttat ggaaagaaca cttgttatta tcaagccaga cggcgtgaga
    27841 cgccatttag tcggaagcat catccaacgt tttgaagcaa agggactggc cattgctgaa
    27901 atgaagtttg aaactatgac tcctgaatta gcaaaagaac attatcagca cttgactgag
    27961 cgcagttttt ttgatgaact gatcgattac atgacttccg gtcctgtcgt ttatcttgtg
    28021 ctggtcggcg aggaagtgat cgatatcgtc cgtaagatgg ttggagcaac aaaagctgcc
    28081 gatgctgttc caggtacgat ccgaggagac tacgcgctgc cggggactga aaacatcatt
    28141 cacgcttctg attcccgtga tgctgcggta aaagagatcg cccgcttttt cccgccattc
    28201 gatataaaca aaccgtctga aaaagcggcg aagtgataca aattctaaac tgctggtcta
    28261 gttgctgggt tcagcaatgg ccagcagttt tttctctaag gacctccgtt gattctttcc
    28321 taacatatat tcgagcactg tgaagaattc ttggtagtct ttcgtctttt cttcccctaa
    28381 agcagcagaa atcaatcttt gataataatc tgccatcaat gtttcataga tcactgtact
    28441 tctttcttta tgtgctaagc tccgtttcat atcttcattc acactacgtg cctttttcaa
    28501 ccgaccattt tctatataaa acgaagtcag gttcattaaa aaagtaaaga gatactgctc
    28561 atgattgttc aactgtgcta atcgtcgcat tctttttttg attttcaaat aattgttctc
    28621 aatgaaccga tcactgaata tccaaggaaa atcagctaga atagatagtt caaattgacc
    28681 atagtctggt atctgcatca agtattcttc aatgattcgg cattcataac gtaaaaattc
    28741 aaattctgtc tcagatactt ctgttttttc gaccatgtga agattcaaaa ccaaataata
    28801 cagatcttct gttttcttaa aatgcctacg gttattgctg atctctttat aaggatcaaa
    28861 gctgtcttct tgtacccgat ctagcattcg tctttttttc tttaacttta ttggttcatc
    28921 cagctggttt cgcatccatt catattctgc aagtgtcatg ttcattcgct ctaaaaatga
    28981 tctcaatgtt acaaatgaca tttttttcac tgaatttgtc tcaatcccga ctagagaagt
    29041 cttaaaacag attccttccg ctaattggga ctgtgtcaac ttcttgttga ctcgaagttt
    29101 cttgatcaat tcaccatcat acataatagt tctccttttt aatttaaatt tttccacatt
    29161 caataaatag atacgttaaa aaggaaaaat acacgaaaaa aataaaaaaa tgtttttttc
    29221 ataaaaaata ccaaccttca agcattagaa tgttgtctaa ctactgaagg ttggtttgat
    29281 tgtttattga ttattttttt gctgttcaac gtctgttctt gttactggat ataagttggt
    29341 tgttgtttta ttaccatttt ttgaaaagac acttgtcgaa tgttcgcctt tttgcttttc
    29401 gattgcttca ttacgttccg cttcattttt atagtcatat tttttcggat caatcgtctc
    29461 aaatccgttt ggcacataaa accgcaagag attttcctga ttcagcttat ctgaaagact
    29521 taacgcagtc ttgacttttt cctgatcttt ggagatttcc tcatcgattc ctgctgcttt
    29581 cgtatcgatg atttctcctg tctgattatt gtaagctttt cctcctagga cggtgtattt
    29641 gggtgtcaca aagttcccat ttcgaaaagc aactacttga tcatgctgag aagataataa
    29701 gtctgtacca aaatgaagat aatctttatc atcgattccc aatagatgta gtaatgtcgg
    29761 caaaacatcg atttctccac cgtattcatg attgactgtc cctttagtat aaccaggcat
    29821 atggatcatc aatggcactc gttgcatctg tgcgttgtca aattcatccc atgtatctgg
    29881 atctttacca agagcgcttg ctaaatcttt attatctgta ttcgagatcc caaaatgatc
    29941 cccgtaaatc acaaagattg aattttggta aataccagat tttttcaaat acgtaaaaaa
    30001 ttgttccaat gattgatcca gataatgtgc cgttcgaaca taattatcta ctgtgctatt
    30061 gccagtattc aaagatggaa agtcgaaatt cttgtcatcc gtataataag gcgtgtggtt
    30121 ggttacggaa agaaatttcg tgtaaaacgg ctgttgtaga tgctcaagat attgggcaga
    30181 ttctctgaat aaatctttat ctaatatccc ataacctaac gtttcgtcgg attcatcaaa
    30241 ataagatcga tcaaagaaat tgtcataacc taagttttta tatacatgat ctctattcca
    30301 aaaactagct acattcccat ggaaaacagc gcttgtatag ccttgctttt gtccaaggat
    30361 cgctggtgcg gcttgaaaga cattgtctga cccaagttct gtgaataatg agccttgagg
    30421 taaaccaaat gtgccagttt ccaacatatt ttcagcatca cttgtctttc cctgtcctac
    30481 ttcatgaaag aaattgtcaa agctgatcgt tgcttgattc tggaaaatac tatttaaaaa
    30541 cggtgtgact tcttgtccgt ctaccttcat attaatcaaa aactgctgga aactttctaa
    30601 atggagaacg atcacatttt tccctttagc gatcccaaat gtttcgggat ttggctctgc
    30661 ataatgtttc tttgtataat ctaaaacttc atcaatccca ttactgcttg catgcgcgcg
    30721 aacactgcta gtcattcctg ttttgattcc gtcataaata gtatacgcat cgattcctag
    30781 atatttgaca atatatgaac ggtcaaatgt tcgctgcagc aattgtggac ggttggcttc
    30841 gctcagcgct agatttcctg aaaaaataag ccctgaaaga caggtaacag cgatagccat
    30901 cggtttgccg acagggttgc ttttgatagg tattttcttg accttcaacc aaaccagtag
    30961 cccaatgaac acagcgatat ccagccagta gatgacatca tggggcttca tcaaagaaaa
    31021 ggagcttcct gacaatcctt gcgacacttt cgaaaagcct aatacagatt tgactgtaat
    31081 gaaatctgtg aattcccgat aaaaaataat attcaagtac aacaaaagcg tatttaaaat
    31141 atcaatgatc aaaagaatag ctaacgctgg ctttggcttt ttaatataca aagaaaggcc
    31201 aaaaaacaat aaggtagtcg caattgggtt gatccacaag ataaagtact ggatcgtacc
    31261 ttccacacct aatgaaaaat ctgcatagta ggcaatgatc gtcttcagcc agaaaaagaa
    31321 aacagctgtc aaaacgattg ctaaacgatt ttggcacaat tttttaagtt gtgtcacgta
    31381 tatcacttct ctctattcac attttttctt attttgctcc tgaaaaatga aaatttccta
    31441 aaaaagttgt tatcttaaga tttttttatt ctttctaaag attattccgt ccacttttca
    31501 cagcataaaa ccgctgcgat gtaagcgatt aaactaatga agagataaaa gtcttgtggt
    31561 gaccgcttca ctccacggta ttcaaaagcc agtttctcgg tattcgtaca aaaaaagagg
    31621 ctgtgacaaa attcttgtcg cagctcctta tttcttgatg caggacagct ttttcacaac
    31681 ttcttcctta aacggtgttc tcctgccaac tcctcggcca caagtcacaa tatttataaa
    31741 acatgagaag ctgttttttg aaattgttcc ttgtggctca gagttaaacg gcaggctcaa
    31801 gacctcttga tacacggtgt tcaatcagct gaccctcggt attagattat gtcaggacgc
    31861 taattttaca acctcttggt aaacggtgtt caaaagcttg catctgcggt aataagccca
    31921 accaaacgaa aaatataagg agctattttt tgaaattgct ccttgtggct cagagttaaa
    31981 cggcaggctc aagacctctt gatacacggt gttcaatcag ctgaccttcg gtattagctc
    32041 aaaaaacggc aaaatatgag gaactatttt gccgtttgtt tctctaatac tcaggtcaaa
    32101 acgctgattt cacaacctct gtttttcatt gtccatagga ttgatattgt ccttgtgatg
    32161 actcattcgt aataattgag gcttgatttt cttcacttcc cgttatcgtt tgtccactgc
    32221 cagaatctga aggacttgtt cctgtcgact cgtttttttg agaagagaca gttgttttct
    32281 tgacttgttc atttgtcttt ttccattccg ctaaacgatc ggcgaccact tttttgtcta
    32341 ctttttcaga ataagcggtg atcacttttc catttttcaa acgggctact ttgtcgccat
    32401 catgagtcaa aagcaaaacg gtcaaatgct tgtctttaaa ttgatattgt ctgagagaaa
    32461 tcgcactaag tacgtcatcg atattaataa attgattttc ttcttttcct tttggcaaat
    32521 aaatgtccaa ttcgcctttt tcgtaattgg cagttatttt atactccgcc caccaataag
    32581 ttgagaattc ttttttgaaa ttatctactg attctttgct tgctacgatt ttttctgttt
    32641 ctttctgagc aagatctgct gtcatttttc ctgaaaatgc tgcggataaa ataagcagta
    32701 agaaaaaacc gaataaccaa aagagaagaa acggtctttt tttcctcatg ctttcctcca
    32761 ctcctattta attttgacta ttctattata tacaaaaaat gaaatagaaa gacaaaaaag
    32821 ttgaaaaaga tcgtaaataa ttgaacttct ttttcacaaa atggtaagaa tggagaattt
    32881 cacgcatttg ctctcaacta ttctttctta agcctcaaat ccgtttttat ctgatacgtc
    32941 tttgaaccaa tgacccgatt ttttgattgt tctttttcca tcttgtgcta agtctactga
    33001 aataaagcca tatcgattct tatatgcatt ggtccacgac cagttatcca tacatgtcca
    33061 catatgatag cctaaacaat tggcaccttc ttgaatggct tgatggacat atttcagatg
    33121 agattggaca aattcgatcc gataatcatc gtggatcatg ccatcttctt tgataaatcg
    33181 tgcttcgttt tctacaccca taccgttttc agaaataaag cagtcgatat ttccataatg
    33241 atctcgtaca ttgatcaaaa tatcatagat tccttgttca tagatctccc atccgcgata
    33301 aggattcatt ttacgaccag gcatttcata attgtcgaaa taatcctcag gcattggacc
    33361 gtttttagta tcgattggtg tttcttttgc ttttacccgg cgtggctggt aataattgat
    33421 acctaaaaga tctactgtat tttctttgat aaccgtcaaa tcctctgcat ggatcacagg
    33481 catgagatca agttctttta ctatctcaat caattcactt ggaaactcgc cttttacagc
    33541 tggatcaagg aatgaacgat tgaagaacgc atctgctatt tgcgctgctt taacatcttc
    33601 agggtttgat tcgtctcttg gatagcttgg tgtcagattc aagatgatac caataacacc
    33661 accttgctcc atttcatggt aagatttgat ggctaacgct gaagccaacg cttcatgata
    33721 gccaacttgg atcgcttttt tcatatctga ctcttctgga taatgccatc gatataaata
    33781 acctgcttcg actggaacga ttggttcatt atgagtaaac cattttttta ctttcgaacc
    33841 aaacaattca aaacaacttt tggcatattt ttcataggct tcaaccgttt ctcggttcaa
    33901 ccatccgcct ttttcttgca gatacatcgg catatcgaaa tgatacagat tcacaaaggg
    33961 ttcgataccg ttttcaatca actcatcaaa ataacgatga tagaaacgaa cggcttcttc
    34021 gttgattgtt ccagattcat caggtatcaa acggctccat tggatagatg ttcgaaatga
    34081 attatgccca gtctgtttca tcaactgtac atcctcttga tacttcgtat aaacttgcga
    34141 ggttttttca ggtcctactt gatcaaagaa cttttgcggt tcttttttgt accaataatc
    34201 aaaaatgttc tctccttttc catcgcctga aatccttcct tctgtttgtg ggccacttgc
    34261 tgcagaaccc caccaaaaat ttgctggaaa ttgatatttt ttcatcaaag cacctctttt
    34321 tcttttttag ttcgttttag ttgattccat gtatgatatt ttccatcaca atcacttttt
    34381 cattttcttc ctcttctttt ttgattcgtt ttaacagctg ttctgctgct cgttccccga
    34441 ttttatctgt tggtggcgtg atcgtgattt tcggaaggat actcagatcg tttttatcta
    34501 aataatcata gtgactgaac gcaatatcct tcccgattct gacaccttct tgattgaaac
    34561 attcgatggc accaatcgac atattgtaat tgctgacaaa aatagcagtt gccccttttt
    34621 ctaataatgc ttttgccccg taatagccgc tttttctaga atagtctcct gtaaagacct
    34681 ctaattggat cgatggatca ttttggaccg cttctttggc tcctgctaaa cgttctcttg
    34741 ctacgtagtc tgtttgagtg gcagcaataa atccgatttt tttatgtcct tgttcaccat
    34801 gatgagcgat cacttttgct acactttctc gatcattcac taatattgta tcaatacctg
    34861 ctggtccttc tggacaattc aatgcgataa ctggaatact catttttgtg agcatttcaa
    34921 atccagacca ctcttcttcg gataagaaga caatcagccc atcgatcaca tgcttcatca
    34981 ggtattcaat tttttgttca attttttctg gctgatcggc aaacccgctt aataataagc
    35041 tatacccatt ttcttcagct actctttcga tccctgagac aacttccgac ccaaaacggc
    35101 tggaaatccc gtttatcaaa agtccaatag agaagcttcg attattcttc aaccctttag
    35161 caatgatgtt ttctttatag tccaacgctg aaatcgcttc tgctattttt ttcatgttct
    35221 tttccttcag ttgctgacca ttcaagtaac gggaaactgt tcccactgaa acaccagcca
    35281 tcttagccac atcacggatc gatgccatac attctcactc ctgccagtta catttgattt
    35341 ctctttcttg ttgttcgatt atgatgaccg cattc
//
LOCUS       DACDSY010000008        34866 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDSY010000008 DACDSY010000000
VERSION     DACDSY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.008"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            98..676
                     /locus_tag="IWK44_000390"
     CDS             98..676
                     /locus_tag="IWK44_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9548946.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            676..1605
                     /locus_tag="IWK44_000391"
     CDS             676..1605
                     /locus_tag="IWK44_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9548947.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1602..3338
                     /locus_tag="IWK44_000392"
     CDS             1602..3338
                     /locus_tag="IWK44_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9548948.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3401..4237)
                     /gene="pheA"
                     /locus_tag="IWK44_000393"
     CDS             complement(3401..4237)
                     /gene="pheA"
                     /locus_tag="IWK44_000393"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP9548949.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(4234..4743)
                     /locus_tag="IWK44_000394"
     CDS             complement(4234..4743)
                     /locus_tag="IWK44_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP9548950.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(4736..6031)
                     /gene="aroA"
                     /locus_tag="IWK44_000395"
     CDS             complement(4736..6031)
                     /gene="aroA"
                     /locus_tag="IWK44_000395"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP9548951.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(6044..7135)
                     /locus_tag="IWK44_000396"
     CDS             complement(6044..7135)
                     /locus_tag="IWK44_000396"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP9548952.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(7152..8318)
                     /gene="aroC"
                     /locus_tag="IWK44_000397"
     CDS             complement(7152..8318)
                     /gene="aroC"
                     /locus_tag="IWK44_000397"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP9548953.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(8320..9384)
                     /gene="aroB"
                     /locus_tag="IWK44_000398"
     CDS             complement(8320..9384)
                     /gene="aroB"
                     /locus_tag="IWK44_000398"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP9548954.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(9417..10439)
                     /gene="aroF"
                     /locus_tag="IWK44_000399"
     CDS             complement(9417..10439)
                     /gene="aroF"
                     /locus_tag="IWK44_000399"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP9548955.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(11098..12072)
                     /locus_tag="IWK44_000400"
     CDS             complement(11098..12072)
                     /locus_tag="IWK44_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP9548956.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(12053..13936)
                     /locus_tag="IWK44_000401"
     CDS             complement(12053..13936)
                     /locus_tag="IWK44_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP9548957.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(13954..16179)
                     /locus_tag="IWK44_000402"
     CDS             complement(13954..16179)
                     /locus_tag="IWK44_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9548958.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            16294..17328
                     /locus_tag="IWK44_000403"
     CDS             16294..17328
                     /locus_tag="IWK44_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9548959.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(17395..18408)
                     /locus_tag="IWK44_000404"
     CDS             complement(17395..18408)
                     /locus_tag="IWK44_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9548960.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(18486..18674)
                     /locus_tag="IWK44_000405"
     CDS             complement(18486..18674)
                     /locus_tag="IWK44_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP9548961.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(19114..19242)
                     /locus_tag="IWK44_000406"
     CDS             complement(19114..19242)
                     /locus_tag="IWK44_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9548962.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(19245..20618)
                     /gene="rlmD"
                     /locus_tag="IWK44_000407"
     CDS             complement(19245..20618)
                     /gene="rlmD"
                     /locus_tag="IWK44_000407"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9548963.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(20729..21784)
                     /locus_tag="IWK44_000408"
     CDS             complement(20729..21784)
                     /locus_tag="IWK44_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9548964.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(21794..23224)
                     /gene="gatB"
                     /locus_tag="IWK44_000409"
     CDS             complement(21794..23224)
                     /gene="gatB"
                     /locus_tag="IWK44_000409"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP9548965.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(23221..24693)
                     /gene="gatA"
                     /locus_tag="IWK44_000410"
     CDS             complement(23221..24693)
                     /gene="gatA"
                     /locus_tag="IWK44_000410"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP9548966.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(24693..24998)
                     /gene="gatC"
                     /locus_tag="IWK44_000411"
     CDS             complement(24693..24998)
                     /gene="gatC"
                     /locus_tag="IWK44_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP9548967.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(25104..27140)
                     /gene="ligA"
                     /locus_tag="IWK44_000412"
     CDS             complement(25104..27140)
                     /gene="ligA"
                     /locus_tag="IWK44_000412"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP9548968.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(27157..29391)
                     /gene="pcrA"
                     /locus_tag="IWK44_000413"
     CDS             complement(27157..29391)
                     /gene="pcrA"
                     /locus_tag="IWK44_000413"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP9548969.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            29546..31105
                     /locus_tag="IWK44_000414"
     CDS             29546..31105
                     /locus_tag="IWK44_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP9548970.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(31216..33147)
                     /locus_tag="IWK44_000415"
     CDS             complement(31216..33147)
                     /locus_tag="IWK44_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9548971.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(33165..34079)
                     /gene="pfkB"
                     /locus_tag="IWK44_000416"
     CDS             complement(33165..34079)
                     /gene="pfkB"
                     /locus_tag="IWK44_000416"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9548972.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(34076..34828)
                     /locus_tag="IWK44_000417"
     CDS             complement(34076..34828)
                     /locus_tag="IWK44_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9548973.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
BASE COUNT         9408 a         7588 c         6384 g        11486 t
ORIGIN      
        1 aaatggtgag gtttctttaa aataaaaata tccttgcatt ttctcctaga aataagtatt
       61 tttaaattac aagactattc ttaaggcggg taaaaaaatg ccaatacgcg taattcttat
      121 cgatgatgaa cagacgattt tagatggaat gaaacagttg atcaattggt ctgagcacgg
      181 gtttcaatta atagggactt tttccagtcc gctgaaagcg cttgattttt ctttggtaca
      241 tgcaatcgat atcgtggtca cagatgtaat gatgcctgaa ttaaatggaa tcgaattatc
      301 gcggctatta aaacagcaac aacctgaaac acagatcctt atcctctcta gctatgatga
      361 atttgatatg gttaaagatt cttttaaaga aggcgtcgct gattatcttt taaagcctcg
      421 attgagtcct gatttttttc taaattcctt aactgccgtt tcacaaaaaa tcaaaaaaaa
      481 gcccaacaaa agcccaatca ccaatcgtta tgagcagatc tgcgagaatc tttcacagca
      541 gattgctgga caaactcgtc cattgcttga ccctcttctt tttgaacaca ccttgttttt
      601 ctgttatata ccgaaaaacg gccattcagt tcaaaacaag gctctaaaca aaaacagtcg
      661 atcacccatt cattgatgga tagcgacgat tcgtttacta tttatcctgt ttctaccttt
      721 tcggaagaat ccggctatgt aatcaatact gattccaaag atacacttag gaagtttgtt
      781 caaaagtttc gtcaagaagt ttttccaggt gatttttttg ttgtttctga ttcttttgca
      841 atccaagata tcaatgaaat attccaagag atcagaaatt gcaccaaagg acaacgtttc
      901 tatcataagg gaaagaacct tgcttatcag catgaacttc ttccgttatt aaccaaccac
      961 gagcagcaag caagtagtta tttaacaaaa atcgttcata aagactacgc tttagctgtc
     1021 aaagaacttg agaatttctt cacgacgata ttggaacagc ctattgatcc agcttatttg
     1081 aaacatgagg caatcaatcg tttctatgca ttactcaacg ctttggctga cgagcatgtt
     1141 gggaatgaag aactttcttt gctcaagatc gcccttcctt ctttattagc cgatgctgag
     1201 tatttcgaag atttttctat tcttgtcaaa gaaacacttg agaaactcac ccacttttct
     1261 gcaaatgcaa aaaaagacaa tttcgatatc ttgactttga tctacgagtt cgtccaggaa
     1321 aattatgaga aagaaatcaa tttacaactt ttatccgaaa aatatcattt ttcatatagc
     1381 tatctctcag ccatctttac tgaaaaattt ggtatcaact tttcaaaata tctaaaaaaa
     1441 gtccgtatca gtaaagccaa gctctttttg acggaaacac ggcttacttt gacggaaatc
     1501 tgtgagaaag tcggctatac cgaactgggt tatttctcca gagtttttaa agaagagaca
     1561 ggaatgacac cttctcaata ccgtaaagga acttttgtaa aatgagaaaa aaaatcaaag
     1621 agcatgaact ctttgccaaa cttctgttca ttttgttcat cggtcttttt ctgcaagcca
     1681 tcgtcatttc tttgtttatt tatcatcgat caagggatgc ctatatccag ctgttcaatc
     1741 aatcgaatga tgtggtacta aaaaagattc aatctgaatt tgaaagtttg aatgatacaa
     1801 tcgagaatac attagccgct tttgattcta atccagctgt caaaagctat ttttccaatg
     1861 atccagccca acatatggaa cagcttcagc aactaagaac aatccaaaaa atgaatgact
     1921 ctctttcaaa aatccatcca atgatcgatt acgatgtatt gatctttgga gaaaatggaa
     1981 gaacttttgt cggaaacgac atgctcactg ccgtatctgc cgattctttt tttcaatctg
     2041 cgattgctca gagagtgaat gaacgtgctg ctgatacaca aatgcttttt gcacaccacg
     2101 gattgactct tcgcgataag aaagcaccca gcgtcttctt tgtcaaaaaa ctgaaaaata
     2161 cattgaatca cgtttacgga tatgctgttt tatcgatctc atccaagcaa ctagcgaatc
     2221 tttttcaatc tgtagtaaat cctgagatat cgaagatctc cttcatgaac gaagaacaga
     2281 cgatcatcgc atcaaatgaa gaaaatacaa ttgggagccg atcaatgatt ttcaaaaaac
     2341 taacagtagg tgaaacaacg atttcaaatg gacagcagtt gacccgtctt cctctgtacc
     2401 gccaggattg tacattactc agtcaagtcg atcttcattc tttatctaac caaatgggcg
     2461 tgatttttcc gattattttg tataatatcg tgacacttgc ttttgctggc tcattggtat
     2521 ttatgtattt ggatcgacac acaaaatcga tctaccgctt gattgattcg ctaaaacggc
     2581 tagaggaaga tggcttaaaa gctactgttc ctgtacaagg aagccatgaa gtccaattgc
     2641 tagggaatac gatcaatcaa ttgctaagta atgctcagca tcattatgaa cagacgatcc
     2701 aagacgaaaa gaagaaacgt tcgcttgaaa tccaaactat gcaagcacag atacagcccc
     2761 attttattta taatacgctg actagcttga aatttttgat ttggcagaaa aaaacagatg
     2821 aagcagttca aggaatcgac agtttcatta ctttactaag aagtacgatt gggaaaaaag
     2881 aagaagtgat tccagtcaag gaagaaatca aaagcgtaca aagttacatc gatcttttga
     2941 gtatgcgata tggcgaaaga atctcggcta aaataatgat tcctgatgaa ctgcagtcat
     3001 tatctatgcc gaatatgatc cttcaaccaa ttatcgaaaa cgcctatctt catgcttttc
     3061 acaataaaac gcaaggattc atcacggtgt acggcaaaac aaaaaatcag cttcttttat
     3121 tcgaagtgat tgataacggc gatggcttcg atactgaaaa taccaaacaa aaaaatgatc
     3181 atttttcagg aatcggcatc agccatgtag atgagcggat acgcctaatg tatggcgatg
     3241 catatggctt aacgatccaa tcagtgattg ggacaggaac gactgtaacg atttgtctac
     3301 cgcttcttcc taaaaatcaa tcagaatatc aaaaataaag aatagccaag cgctgatttt
     3361 tttattttaa gttaaataaa tcaatcagta ttcttgactt tcatctacgc gtctcttcta
     3421 ctgtcagcac tggataacag cccaatattt gactttctcc tccgagcatc ttgatttctt
     3481 ggagcgcatt ctctactaat gtcattggac ggtccaacaa caaatcgatc acgaaaaaat
     3541 attctcccaa gctagttttc aatggtcggg attcaatctt acttaagttg atttcccgcc
     3601 agccaaaagc tgccagcatt ttatgaagca tccctggccg attagcaggt agtgtcaaga
     3661 tgacactcat ttttactgga gctgactgct ggctggtcat ctttcggtca ccgacgatcc
     3721 aaaaacgtgt ctggttcaac tcattatctt ggatattctc agctaagatc tccaatccaa
     3781 catgctgtgc tgtttctaat gaagcaatcg ctgctgcgtc ttctttcgga tgttctgcta
     3841 catacattgc tgcagcagtt gttgattctg ttgctactaa aggaacatta ggataatgcg
     3901 tttctaaaaa ctgttgtgat tgtgctagtg cttgtggatg tgaaaggatt tttgtaatct
     3961 ttgttgctgg attgcctaaa agctgctgct tgataggaag aacgatttca cttttaactt
     4021 cgacttctgg atgtttggaa agaagatcga ttgtgtgatg aacagctcct tccaatgaat
     4081 tctctacagg aacgacagcc agatcgactt gtttgcgaaa caaagcgcgc aaacaaacag
     4141 ggatggaagc atacgcagtc aattgttctg taggaaatag ctgacaagcc gcctgataag
     4201 tgaatgaact ttctggccct agatagctga cgttcatatg cttttgatcc tttctataat
     4261 ggtatcgatg atcttttctg gtgtttgatt ggtcgtgttg attcgatgtg tcgcactttc
     4321 ttcataccaa gaaagtcgtt gtgccaataa tgcttttatt tcttctcttg tttttccaag
     4381 agctaaaggt cgaatagacg tttcatcttg ttggatacga tcaactagac agtccgcttc
     4441 tgcctctaag taaagaacaa tcggctgtgt ttttaaaaaa cgccgatttt ctgcactttg
     4501 gacaatccct ccaccagtcg cgataatttt gtcttcattt gacagctttc ttaaaatatc
     4561 tgactccact tttcgaaagc ttttttcacc gtaatagtga aaatattcgg caatcgacaa
     4621 ttggatatgt tcctcaatct ttgtatctaa atctacatac tctttttgta aacattgagc
     4681 taacccttta ccgattgttg tttttccagc gcccatgaaa ccaatcagca aaatgctagc
     4741 catgagcttc tcctcctgac acaagttctg ctaaatcgtc aaaaaatgcc ggataagaga
     4801 ttgaaacagc ttcagctcct tctagttcgc aaggtccatc agctatcaaa gcagctactt
     4861 gcagcatcat accgattcga tgatcgcctc tgctggacac ttttgcgcca tgcagaggtg
     4921 tcggcccatg gatgatcaga ccgtctgctg tcgcttttat ctttgctccc attttctgta
     4981 attcttctgc caccgcatca attctgttgg tttcttttac ttttaattct tctgcgtctt
     5041 tgatgatcgt gattccttct gcttgagtag ccactaaagc tagaatgggc aattcatcaa
     5101 tcagacgcgg aatgatttct ccaccaatcg ttgctttctt caaatgactc gtcttgacac
     5161 ttaaatcagc cgattggttc tgttcattgt gattcgtctg ggtaatcgct gcgcccatct
     5221 cctccaacac atctaagata ccagttcttg ttggattgac cccgacattt tttaacaaca
     5281 gttggctttc cggaacaaga agtcctgctg ctagaaagaa agcagctgat gaaatatcac
     5341 ctggaacagt tatctgttga ccagttaatt tttgcggtcc ggtcactatg atcgttttat
     5401 cttccactgt gatccttccg ccaaattgac gaatcatttc ttccgtatgg ttacgggaac
     5461 gttctttttc aacgattacg gatgtgcctt ctgcttgtaa tgccgcaaac agcaaagcag
     5521 atttgacttg cgcactggct acaggcatat tgtatcggat aggtgataaa gagcgggttc
     5581 ctgtcacagt gattggtaga tactggtcat tttcttctcc ttgcaagtca gcgcccatct
     5641 cgcgtaaagg ttccatcact cgccccattg gacgtttaga caacgaagca tccccgaaca
     5701 gagtagtcgt aaaaggctgt ccagccaaaa tacccatcaa taaacgtatg gtcgtaccgg
     5761 agtttcccat gtcaattgat tcggttggtt ggacaaatga gtggctaccg cgaccatgaa
     5821 tcttgatcac ctcttcctct tcttcgatct cgacacctaa ttgttggaag gcatgtaaag
     5881 tactcagaca atcttctgcg cgcaaaaaat tttgtatagt cgtcgttcct tcagcaattg
     5941 ctccaaacat gatgctgcga tgggaaattg atttatctgc tggtatccta acagtccctc
     6001 ttaatccatg tatttgttgt aataattgca tttatccagc ctcctatttg atccagcaat
     6061 gataactggt attagacata atgattcttt ttgcttgtaa caaatcctgt tggtttttaa
     6121 aactgatttg cagtattcct aaaatatctt ccctggtctc ttggatcttc aaattaatca
     6181 aagaaactcc cgcttttccg atcagtccag ttacttctgc aataacgcca ggttcatctg
     6241 gcacatcgat aaataaatca aaaaaggcag gaatcgcgcc ttgcttatgg acaggcaact
     6301 ggtctcgact ttcttttgcc cgattgaaaa aagaaaagat ttccgattgg ttctcagtca
     6361 tgatccattc tgctatttgt gtcatttctt gttgccaatc tgccaatcgt tccaataata
     6421 tctgcttatt gctcaacaaa atatctgtcc acatgacagg atctgaggaa gcgatcctag
     6481 tgatatctcg aaagcctcct gctgccagtt gtttggctcg cggatactct tgattgaatt
     6541 gttctgcctg attgactaag ccagctgcca gaatatgtgg aaaatgactt agcattcctg
     6601 ttatacggtc atgttctctt gctgtaagag ttacatattt tgctttggtt ccgcaaaata
     6661 gttcttgcag tttttcaaca tcttttttcg tcttttccaa aggtgtaaag atgtaatagg
     6721 cattttcaaa tagtttagca tctgctgcct gtatacctga tttatgagaa ccagccattg
     6781 gatgtccacc gacaaaccga agatttttct gctcagcgga tgtcacgact tgctgcttcg
     6841 tactgctgac atccgtaatg atgacgtgat cagacaagtt gattctttca aggacatcaa
     6901 gataagctaa ggttgtacgg acgggaacgg ctaataagat aacgtctgct tgttctgcac
     6961 ctgtttcaaa atcacttgca acgtaatcaa cgatctgctg ttctactgct tgctcacggg
     7021 tttttgactg actatcccaa ccaactacta tcagttctgg attggtctga cgaatacaaa
     7081 gtgccataga agcaccaatc agtccaagcc ccacgataaa aacatgttgt gccatcttat
     7141 ttgccccctt tttagtaatg tttcaagtag tcacggtact ccttcactgc agctttcaac
     7201 tcaacaagcg aatcacttgg gaattttgac aatagctctg tcgctaccac agtggctaca
     7261 acattttccg caacgacgct agctgcagga actgctgtac tatccgaacg ttctacactt
     7321 gctttgtaag gttcttttgt atcgatatca acactttgaa gtggcttgta caatgtagga
     7381 atgggtttca tgaccccgcg aatgacaagt ggttcgccat tggtcattcc gccttcgaag
     7441 ccgcctagat tattggtacg tcggaaatag cctctttctt cttcccatag gatctcatcc
     7501 atcacttctg agcctggtaa tgaaccagaa gtgaatccga ccccaaattc tacgccttta
     7561 aaggcattga tactaaccac agcttgtgcg attttggcat ctagcttcgt gtcccattgt
     7621 acgtagcttc ctaatccagc tggaacgcct ccaacgagca cttctacaat tcctccgatc
     7681 gtatctccat ttttctttgt ctgatcgatc aaatctttta ttctttgctc aactgctgga
     7741 tcaacaactc gtacttctga cgcttcagaa atttctttga tttcttgaat cgagatattt
     7801 ccaggtattt ctgcttggat tccaccaaga caaatcacgt gtcctgctac gatgatgtca
     7861 agttcttcca gaattttctt ggcaattgcg ccgattgcta cacgcatggt cgtctctctt
     7921 gcagaggacc gttcaagcac attccgaaga tctcgatgat ggtatttcat accgcctacc
     7981 aaatccgcgt gtcctggacg cggacgtgca actcgccgca gtgattggtc tttttcaggc
     8041 acttctgcta cagacatgac agttgtccaa tttttccaat ctttattttc taccactaaa
     8101 gtgacaggtg aaccaattgt tttgccgtga cggatgccag acgtgatccg aacacgatct
     8161 ttttcgatct tcatccttcc tcctctgccg taaccagctt gtctgcgagc gagttcgtga
     8221 ttgatttctt cttctgacag aggtaatcct gctggtagtc cttcaataat ggctgttaat
     8281 tctggtccat gggattctcc cgcagtcata taacgcatct tacagtcctc ctaagtaatc
     8341 tttgaatgtc tctgtgggaa caggaacgat ttttgcttgc ccaatctttt ctagcaagat
     8401 gatattcaat tggcttcctc ttgctttctt atcatgggtt atcgcgtgga ataattcatc
     8461 tggatgactt agatcgtacg taatcggcag atggaatttt tgaagtatcc tttctaaagc
     8521 agctgtcgat ccagttggag acaagccgat tttttctgca tgagaagtga tggctaccat
     8581 tccaatagct actccttctc catgactgac taccccatat cctgctgtat tttcaattgc
     8641 atggccaatt gtatgaccaa aatttaaaat aagtcgattt ccttggtcaa agacatcttc
     8701 ttccactact tttcgtttga tttccaacgt agcagtaatg attggaacag catgttccag
     8761 aagatctttt tcgtctttca gctcgttcag ctgtttccaa agcgttgcat ctgctatagc
     8821 tgctgatttg atgatttcag cgattccttc tcgtaaacga cgtaccggca aagtttttag
     8881 aacctcagga tcaatcaata caccagctgg ctggctaaat gttccgacaa ggtttttcgc
     8941 tttgtttgta ttgactgctg tcttcccgcc aatactagaa tctacttgtg caagtagcgt
     9001 agtcggaatt tgaacgaaat ggatcccacg catgaacgtc gaggccacga atgctgctag
     9061 atcacccacc attcctccac caagtgcaag tatcccatcg ctgcgggtaa aatcattatc
     9121 tgctaaaaaa tcgtataact cagctgcttg ttctaaagat ttactggtct cacccgccgg
     9181 tacgattgaa aggactggtt caaaattagc ttgtctcaat tggaacatga ttttttctgc
     9241 atatagtgga gcaacgtttg tatcagtgat cacagctact ttttgaggtc tccaaatcgt
     9301 ttgaagccat gatccagcct gttctagtgc gcctttttca atatgaatat cgtatttttg
     9361 gttatcaaca tgtacagtaa gcattcgttt cacttctttc ttttaaaaag ctggaattaa
     9421 tcgagtgtct gaatcttttc cattgcttct ttcatgatcg caacttcttt catcatgcgt
     9481 gagtaagtct tttcattcaa cgattgggga ccgtctgacc aagcatttgc aggatcagga
     9541 tggatctcta cgatcatgcc atctgctccg cttgccacac ccgcacgagc cattggtggt
     9601 acaagttccc aaattcctgt accatgactt ggatcaacaa tcacagggaa atgagttaat
     9661 tttttcatca atgggacagc acttaaatca aacgtattac gtgtcgctgt ttcgtaagtc
     9721 cgaatcccac gttcgataaa aatgacagga gatttgtctt cgactgcaat gtattctgca
     9781 gcattaagcc attcattgat cgtaccggaa attccacgtt tcaatccaac aggtttacct
     9841 gttttaccga cagcagatag cagtttaaaa ttttgcatat tacgtgcgcc aatctgtagg
     9901 atatcgctgt actcagccac catatcgata tgcccttcat ccatcacttc agtaatcact
     9961 ttcattccaa attcatcagc cgcttgacgg atgtatttca atccttcttc ttctaaacct
    10021 tggaaagcat atggagaagt acgtggtttg aaggcaccac ctcgcagaat ttttgcgccc
    10081 ccggctttgg ccattcgcgc acattctctg atttggtcta atccttcgat agaacaagga
    10141 ccagccatcg tcacgaaact gccgtcacca atttttactc cgtctacatc cacgactgta
    10201 ttgccaggat ggaattctcg agaagtcaat ttgtatgtgt ttaaaatacg cacagcattt
    10261 tctactcctt catagcgtgt aaatgctcct tcttgtaaac ttcgcgtatc accttttagt
    10321 ccaatcacta ctcgttctgt tccttgatcg attgagactt tgaatccttc tttttcgata
    10381 tgatcgacga ctttttttat ttgttcttcc gttgcttctt ttttcataat tactatcatt
    10441 gtttttcccg ctttcttttg tttacttatc tatcttggct gattttctct actttttcaa
    10501 ggtggtttta cattaattcc tactaaatga ccgtgtcctg ttgttctcat tttcgctcct
    10561 tctttcctac ttatgaccca gataatcaac aaaaaaagat gtccctgtag gaaattgcct
    10621 ctacagtgaa catcttcaac gagccttaaa aagcgctgca aaaatataaa atagccacta
    10681 tagatgaatt tctctacgtg gctgtaagtc gtgtttaaaa atctttagcc atcatagata
    10741 caaacctggt ttgtttgtac ccacgaagtc atgaatacaa acctatctaa aatagctaaa
    10801 gtaatacgta ttcagttttg taagttttct agatttcata aaaaatcgct ccaaactgat
    10861 gtaagaattg tacctagtat agacataaaa aaatcgactg tcaatcattt ttatgaaact
    10921 ttttttaatt ttctgatagt taactttgtt tttcagaaaa ataacaattg attttattga
    10981 agaaaatagt catgaacaaa agtatctgag aactttttta ctctcttgtt catgacatat
    11041 agaataaagc agctttgtct tgtaaactcc gtttttcaaa taataatcta aagattgcta
    11101 ctgaataata aagccgattt catggtcctc ttcgctgctg attcggtaag gtgactcaac
    11161 atccgctccc cagctattga tacccccgac tcctcttact tttgcaaaaa tcgtaagaac
    11221 cgttcgtctt tctggcggta gttcttctat atgtgttgcc tgttccagtt cttctgcttt
    11281 gaatggtaaa gcggaaaaag caaatggcgt atcctgctgt tcaaatcgca acgaaaaatc
    11341 ttcttgatct ttatccgtat tatccaacgt ttgatttctt gttagttcta cccatttcgt
    11401 ttccatatgc atgccgcatt cttggggaac aaggtatggg gaaacgggga gtcctgccac
    11461 tttataaata ccttcgacac caccagccat ccgatccgga tatgtttccc ctgataggcc
    11521 ttcatactca aaaaattttg ctttcgttgg cataatgaac cttaaaccca atgcgggtaa
    11581 ttccggtaat cctttttgtc cataataatg catattgaca agcattttac cctctccagc
    11641 gatttggtag gaaattgtca cttgggtttt tggtactgtg ttgatttcca gtgtaaaaat
    11701 gatttcaacc gtttcagcgt attcattact tgaatattga ttatttactg gagcaatcgg
    11761 caaatcgatc tttttattgt ccaccagtac ttctatttct ttgacttttg gatacaatcc
    11821 ggctcctaac cacataccgg atttaaatcc aaatccgcta cctctgtcat tatcggtcgt
    11881 tgctcgccaa aaaactggaa gtgtttcccg gtatagccat tcttttccgt ttttattgag
    11941 cgattccagt ccgcctcttg tataagaaaa aatataatgc gtattccccg atttgacacc
    12001 taatgtacag tcgccgaaga cgattgcaac tttttccgta tccatatgac ttttatttac
    12061 tgtatttttc ataataatat cttacctcct gcatggcggg tttttcttgt ccatctgcaa
    12121 aaacgatccc attccctgag aattcatagt cacttggttt atcgtcaaag tcaccgccat
    12181 agcgcatcac ttctctgcct gtgattggat cagtcacttg gatcgcctga tctttaaaat
    12241 cccatatata gcctccttgg tacatttcat aacgatcaag caacgtcatg taagaatcca
    12301 ttccacctaa cgaattcccc atgtcatgca tatattcaca taagatgaac ggtttttccg
    12361 ctttattttc aaggtagtca atgatctctt ctggtggcgc atacatgcag ctttttacat
    12421 ctgaaatcga atcttcgaat gctggatttg gaaaattccc ttcataatgg acaagtcgat
    12481 cggggtctac ttttttgaag taatcgttca tttgtcgaat gttttcttcc gcataagatt
    12541 catttccaag agaccaaaaa agaatacttg gatggttttt taatgtttcg aaattcgttt
    12601 ttgctcgatc aagaacagcc gcctgccact gaggtactgc acctggtacg ttgtaagaag
    12661 gctcaaatgc gcccattttt tgccatgagc catgggattc gaggttcgtt tccgccatca
    12721 catggattcc tgcttggtcg cataaataat accaagtgat ctggttgggg taatgagatg
    12781 tccgtacgcc gttgatcttt gcatttttta tgatagccaa atctttttcc atatcttcta
    12841 tagtgatggc gcgtcctttt ttcggatgcc actcatgtcg atttaccccg ttgataatca
    12901 tccgttttcc attgaagtag atgattttat ctttgatttc gattttacga aaaccgaagg
    12961 aatagtcaat atactccgtt actctttttt cttcgtctat cgtctccaat tcaagcttat
    13021 ataaattggg ctgaaaatgc ccccacagtg ccacattttc aaatccatcg atgcgaatcg
    13081 tcatttgctc ttttaacgga tattgctgtt gatagatttc cttcccatca ggatctagta
    13141 tcgctacatt cagctgactg tttttggcta cttgctcaaa agacaaatct acttcaagtt
    13201 tgccattttg acatttgtca tctaagcgag gtttgatcca aagatcttct aaatggtttt
    13261 gtcctcgagc aactaagaag acatctctga agatcccaaa aaatcggaaa aagtcctgat
    13321 cttccaagaa tgcagctgta cttcttttgt aaacctccac agctagaata ttttcttctt
    13381 ccaaataagg tgttaaatca aattcagacg gagtgaacga atcttcggca tacccgataa
    13441 aatgaccatt caaccaaata aacattgctt gttctacacc agcaaaatga atcgagattt
    13501 gttttccctt tagaccttct tctaatcgaa aagtcgtcaa atacgaacca accggattgt
    13561 ctttagcagc tgaaaagatc ccttccgcct ctttctcagg gcttgtcgtg aaagggggac
    13621 ggcgaaatac atgtccctcc cacggataca tcgtattgat gtaatggatc ttgtcataat
    13681 ttgctaattc aatatgacta ggaacagtga tttcatcaaa agaacaggca tcaaaatctt
    13741 ttttataaaa tgtttttggt cgtaccgaag gatttggcga ataacaaaaa cgccatgatc
    13801 catttaaaga ctgtttaagg gaggatgtgg tttctacttc ttcccttgtt gcataacaaa
    13861 catgatcgct atgtgcattt gattggttca ctctaaaaac ttgtggatca tctaaccatt
    13921 ttatatttgc tttcatactt ttttccttct ctctcataac tttttgaaat gaagcagttc
    13981 tgtttggaaa tcaccttgaa aatcggggaa gtaaagtcca actgtcatca attcggcccc
    14041 cgaaaatatc cgcttcgagg tctgttcctg ataaagtgcc tcatcatcta accccatcag
    14101 atatacttca tggaacgctg gttgagctga agctagtatc ctccctaaaa acacaatagc
    14161 ttcatcttgt ttaggagaca caaacatcca tgcggcaata ttttcttcaa aaggactcgc
    14221 caaacgataa taatggccat actggatcac cggacgaatc gtttgatagg atataatttg
    14281 gtcttttact tgcttttttt cttcaggaga cagctttgtc aaatccagtt cataaccaaa
    14341 aacagcgctc atagctgttt ctcctcttgt tttcaacgaa gtagatctgt cggtctgatg
    14401 atttggtaca gctgatacat gagctgtgat ggacgaaatc ggataagcaa ggcttgttcc
    14461 atattggatt ttcatacgct ccactgcatc cgtgttatca cttgtccatg actgcggcat
    14521 ataataaata aacccggcat caaaccgtcc acctccacca gaacagcctt cccataagat
    14581 ttccggataa tcagttgtca aatgttcaag tagttcataa agcccgagca tataacgatg
    14641 cgctacttct ccttgtctct gtgggtcaag ggtaatcgaa tagacatctg aaagcgaacg
    14701 gttcatatcc catttgatgt agtcaagagg aatcgtatct aaaatctttc tcatttgttt
    14761 ctcaatcgtc tgccgaacat ctaaccttgt aaaatccagt acatgttgtg atctggaagc
    14821 agaaggcatt ctacctggct cctgcataag aaaatcagga tgtgttcgat acagttcaga
    14881 atcaaccgag atcatttcag gttcaaacca taaaccaaat ttcaatcctt tgctatgagc
    14941 ataatctgct atttcacgta atccatttgt caacttgcct tcatactcga accagtctcc
    15001 taatgaagaa ttgtccgcat ctctttttcc aaaccagcca tcatctaata cgaacatttc
    15061 gatgcctagc tcctttgctt catcaatgat cgccttgatt ttttcactat tgaagtcaaa
    15121 gtaagtggct tcccagttat tgaccagaat cggccgttca gcaaattgat gacgacctct
    15181 tgcgattctt tcacgtagca actcatggtg gatctgactc attttgttca atccctgact
    15241 tgagtaagaa aggatcattt caggtgtttg aaaagactgg cctgcattca attcccacga
    15301 aaaacgatgg ctgttgatcc caccgacgac tctcaattga ttgatctgat ctttttcaag
    15361 ttcgaaagaa tggttgccgg aataaacaaa ttgaagtccg atcgcttctc cagtattttc
    15421 tgtcgtatga tgatgaacta aagcaataaa gttattcata tggtgactgc tcgttcctcg
    15481 acggctttca aatacttttg ttccagataa aacagattgc cgtgaaatct gtctttcatt
    15541 gacatgtgct cctggcaaag caatgacttc atcaaatcgc ctagtatggg caaagtctag
    15601 ctggaaacta gccgcttttt ctagtttgat tgtttctccg gtttcatttc ttatctttac
    15661 agatcgtgtg atcactgctc tatcagtaaa tatggtatag aacaaatcga aataaatcgc
    15721 tgtttctcta tccttaagtg aaatcaccaa cgtttgtgcc tctgacttgt ccatcacata
    15781 agtttgaggc aagccttcga tctctggctt cccatctgta atataatggc tgtcatatct
    15841 cagatccagt aagtctgatc cattcttgcg ctgaatgatc gatgcgggcg ttcgaaagtc
    15901 catgctgcca tgagaagagt attcttgcgg tagtgtatct ttcgacagag accggtcagg
    15961 atttaaggga acatttcctg aaaatccacg atctcttcgg ggataatttt tattgtcttt
    16021 gtactgcttg actggtttgc caaaataaat atgcgccaat acattgcttt cttctatctt
    16081 tatcagataa gaaagaaatg tatttcgtaa atggaagact cggctctttt catcaaataa
    16141 gatgaaattt ttatctgttt ttctatgcgt aatatccatc atttcactcc caacaaaacg
    16201 gaaaccgttt actttttttt ataattcctg tatactgttt actaaacaat tttgtcaata
    16261 aaaatttttt atttttttga ggagtgtgta aaaatggttg gaattagaga tgtcgcacga
    16321 aaggcaaatg tctctcctgc tacagtttcc cgtgtgttga ataacgatca gacgatccat
    16381 gtggctcaga ctactcggca acggatagta gaggcagctg ctgcgttgga ttatgacatc
    16441 agtactcgta aatatatcaa aaagagaatg ccttcgattg gtgtgatctc tacgatcggc
    16501 aaagcgagcg aagaacagga tatctattac aaacatttgc gagtagggat ggaagaagaa
    16561 gctcgacgtc tgcatttagg gatgaatcga atctataatc tatctgataa tcctaaacaa
    16621 tgggaagatc tagatcagtt aggtgcggtg atcgttgttg ggactgttac gaaagaatcg
    16681 attaccgcct tgttaaaaca aaacatgcaa atagtcgtcg tggataaccc tgacatccag
    16741 caagaggtcg atatggtgta tgcagatctt gaacggatga ctaaaaccgt actggaattg
    16801 ttcttagaaa atggtcataa aagaatagcc tatatcgggg gatataaagt cgatatggat
    16861 gaattcggtg ggaagcagat ttctataaat gaaaagagga tgcgtgccta tcagcatttt
    16921 atgatcgatc atcacttaga aaaatatatg tcttatcatc taggtggctg gacagaaaaa
    16981 caaggtgagg tacttgcgac taatctatta gaaaatgaat cccctcttcc tactgccatt
    17041 cttgtcggga gcgatccaat ggcaattggt gtctatcggg cattgaaaca aaaaaatatc
    17101 caaattggag aagagattgt tgtagcaagc tttgataata tcgagacaat cgcaaacctc
    17161 tcccctggct tgaccagtgt tcaaatcaat gcgaaatcaa ttgggcaagc agctgttcga
    17221 atggcattgg agcgaattga cggtatacgg gacgaaccaa tcattttgac ttatcctgca
    17281 cgattgattg ttagggaaag ttttatccca agaacaaaaa ggtattgaaa gagaggttgt
    17341 gaaaagccgc ttcgtttctt catactcaaa acgaaacggc tttttcacaa cctcttactt
    17401 atacttattc acaaaatcca atgtctgctg gtaacaattt tgcgattctt ttagcgtgta
    17461 atcaaattgg tattcatggg taatctcttt ttttgtatct gtataaaaca aggattgcac
    17521 gggtaccttt aattctgtca accgattttc taatgcttga ccttgctcct ggaatgagta
    17581 tgcatttccg tcagttatat acgttggcgg aaattcctta gaaacatggt cagccacact
    17641 agcttctttc aattgctcac tcgttttcca atcttttgtt ccaatcaacg accacgcaac
    17701 ggttttgacg aaaaacttca taaatcgatc atccgaatgc tgctgtgctg ttagcttcaa
    17761 atcaacaggt ccgcaataag aaatggttcc tttcagacta tcttttggaa gtagctgttt
    17821 gattttcatt tgtttagcat acgcatcatt cgtttgtata gctgcatatt gcaatgcgat
    17881 ttgtgaacct gcactatctc caccaaaaaa taattgttcc atgtctagca atttgtcgtc
    17941 tttttcttca gctaataaat ctttgatcag ttcattcact tgaagaactt gattagggta
    18001 ttctgaatca ggcgctaatt cataattcat cgcaaccact gcgatgttgg cgtctgaaac
    18061 taattttgtc gcaaattctt tgacacccga tttatctccg ccgacgaatc ctccaccatg
    18121 tacccaaaat agaacaggaa caggcccctt gctgtttttt ggatagtaga tgtcataagt
    18181 attctcttta tacttggact catacgtcaa atctgttttt ttcgccacgt ttttttctgc
    18241 ttgatcgaac gtttttttat cagtgatttg aacagtattt gaaaacatat ggttgatcaa
    18301 gaatgctcca ggtctggggg aaagctggaa ggcaagaaag attgcaaacg cgatcccaag
    18361 aatgattccg aagaaccatc cgataaattt aagaattttc tttttcataa ggctccctca
    18421 gtagttagta ttaagcattc tgattatacc atagtgttat ttcctatcaa gctctcctag
    18481 atgatttaat gattttcccc aaacttcttc tgcaatgctt ttgacaaaag aaattttttc
    18541 cattgttctt caaacaaaac attttcttct tctgttgaag caaatccaca ttgaggactt
    18601 aagcacaaac gatccaaagg cagatatcgt gtcgcttcgt agatccggtc aatcacttgc
    18661 tggtgctctt ccatttcgcc atttttcgtt acagtcaaaa tctaagacaa ctaatttatc
    18721 ttcagagact ttctctaaaa gaacaaaact cccagcacgt tcactatcgt attctaaata
    18781 ataggcacgg acattttctt gagtaaaata atgggtctgc tacgtcgtca tcccccctga
    18841 tgccgccaaa atcgaataat aatttccgcg tcaaacatgt atatttagtg ttgaatcatt
    18901 tgagaatatg aaaataaaaa tctttttgat caatcagtta atcaaattga aataacgaaa
    18961 aatctgagac agaatgacgt tcattctgac ccagatttga ttttgcacaa ctcaacagtc
    19021 cttattggca aatcgccaaa ataatgtaga ctaactaaaa agactaagaa acaataacaa
    19081 aagttaacac ttcttagtct ttctttagca cgtctattta ttaaagattt tagggccttt
    19141 tctcatgttt ttacgatcat tcttaggtgt ttccatttgt ttggatcggc ctccaccttg
    19201 tttctttttg atcaattctt tcattttttc tttttggtcc atttttattc tcctttaacg
    19261 aatgaagcga ctaattcgat atgatgggtt tgcgggaaaa gatctacagg ctgaactttc
    19321 ttcacggcat aacctgcttc tgcaaataac ttagcatctc ttgcaaaagt tgccggatta
    19381 catgagatat agacgattcg ttctggtgac atttcgattg ctgattcaat aaaatgatga
    19441 tctaatcctt ttcgtggagg gtcaactacg atcacagatg gtttgatccc tttttcttgc
    19501 cattttttca taactttttc tgcttttccg acttcataat gaacattttc gattttatta
    19561 tttaaagcat tgaactgtgc atcttcaatt gcttctggaa taacttccat cccatacacc
    19621 ttatccactc tgtctgctaa agacaagcca atcgtaccta ttcctgaata agcgtcgatc
    19681 acaatgtctt ttttatctaa ctcggcaaac tcgatggctg tttggtacag atactctgct
    19741 tgttgagtat ttacttgata aaatgacttt gctgagattc gataggtttt tcctaatagc
    19801 tgatcttcga tatagtctct gccatataaa actttttctt tgtctcctaa aatcacattt
    19861 gttttttctt cattgatatt ttggatgacc gaaaccactt ctggcaattc ttcatgaata
    19921 gcttcagcaa tttccttgga tttaaagaat ttttctttac gagtaactaa aacgaccatc
    19981 atttcatggg aataatgacc acgacggata atgatgtgac gaagctgtcc ttcatttttt
    20041 tcttcattat atcctcgtac ttggaaccgt tgcaagatat ctctgactac tttgattgct
    20101 tgatcgatga caggatcttg gatataaaaa tcttcgatag ggactagatc atggctattc
    20161 tttttgtaaa aaccggtggc taactgtcca tcgatacggc gaacagggat ctgtgcttta
    20221 ttacggtagc cgaatggttc tgccattccg accgttggaa gtacaggaac ttctggcatc
    20281 ttagcaatct tcgccattac atgctctact tgtttttgtt tgaagacaag ctgttgttca
    20341 tacttcaaat gggctaaagg tgcgatacct atgcgaagta atgcattgtt ttctacaggc
    20401 tgacgatcag gtgattgtgt aataaatgtt tcgacttttc catatccaaa cttattgcct
    20461 gcttttacta ctagtatttc tacttcttct ccagggagtg cattttcgat aaataatgga
    20521 taaccatcga tttttgctac ccccattcct tcataactca agtcgacgat ttcgactgta
    20581 tatcgttcgt ttttctttac tatttgctgt tctgtcatat tttgctcctt taagcctatt
    20641 tttcaatcct actgaactct actataaatc agctaattac acaactaaaa ccggcactga
    20701 ccttactacg ttcagcaccg gcttttgatt aattcttttg attaggtgtt tcgtctgcta
    20761 tttttccgtc accgtcgata tcttcatccg ttaaacgttc gacttctttt acgaattctt
    20821 tgctgacaac ctctaattct tcttcatctg tacctgtgat tgcttcatca ggaatctgat
    20881 cggtattggc aaagaactca atgtgttgat gaagattttg gaaatgcatc ggtgcttccc
    20941 cgccgtattc accatctaag ttgatcatta ctggtcgatt gatatcatct tctgtttcaa
    21001 cgaacaaact gcttgttttg gtatagatta ttcttggatc atcgacatgt cgaccgccat
    21061 tcagcatgag tgctgcaata tgcaagatct caaagatatt tgctgtcttc acaatgatta
    21121 aagaaaactt cccatcatcc aatttggcat ctggcgcgat ctgttcgaat cctccaacag
    21181 agttcgtcag ccctaggaaa aacatcgaag catttccgat atattcccct tcatcatact
    21241 tcaagcgcat tttgattggc tttactcgag ggagcatctc tgctcctttc gcaagataag
    21301 caagataacc aaaaatactc tttagttctg atggaacttc ataagtcaat tctgtcaaat
    21361 agccacctgc agcgatattg atgaagtaac tgttatcaga aaggccgatg tccattttta
    21421 cggtttggtg tttttttatc acttctgctg ctgctttaat attatctcta ggaattttta
    21481 atgctcgagc atagtcgttc gttgtaccag ctgggatgat ggccatttta ggacggtgtt
    21541 caagcggagc gattccatta accacttcgt tgattgtgcc atcgccacca gctgccacta
    21601 ctagatcaaa acctagttct gcgactctac atgcttcatt cttggctgaa tctggttctg
    21661 gtgtagttgc ataggcgctg gcttcaaatc ctgcttcttc taatgcctgt aggatatccg
    21721 ccaaattttt tttcaataat tcttttcccg aggtggggtt ataaattacg cgtgcttttt
    21781 tcattttcag cctctatcgt ttcgctaatt catctttaag tagcttattc acgacaccag
    21841 gatttgcttg tcctttagtt gctttcatga tttgaccgac taggaagcca accgcacgat
    21901 cttttccatt tttgaaatca tcgacagatt gttggttgtt atccaaaatt tcattaatga
    21961 ttggcagtag tttgttcgga tcagataatt gcacccaacc atttttttca actacttctt
    22021 gggcttctcc gccatttgtg atcaattcac ggaagacttt tttggcgatt tttgagctga
    22081 tcgttccatc ggcgatcaaa cgaatcattc ctgctaagtt ctcaggtgtc aattttgtct
    22141 cagctaattc cagtttctca ctgttcaagt aagcagaaac ttcacccatc aaccagttag
    22201 atgcttgctt ggcatctgca ccattttcca aaacagcttc aaaaaaatca gacatttctt
    22261 ttgtaagtgt caataccatt gcgtcatact ctggaagtcc aaactcacgg atatagcgtt
    22321 cgcgacgaga agctggcatc tcaggcaaac tagcttttac tttttgaatc cattcgtcat
    22381 cgatcacgaa tttcgggata tcaggctctg ggaagtaacg ataatcacta gacccttctt
    22441 tgacacgcat caaaactgtt ttattcgttg tttcgtcaaa acggcgtgtt tcttgttgga
    22501 tttctcctcc tgaaagaaga acttttgctt gacgtttttc ttcaaaagct aaaccttttt
    22561 tcacgaaaga taaagagttc aagtttttca attctgcttt cgtaccgaac tcttcttgtc
    22621 cataagggcg aagagaaata ttggcatcac agcgcataga gccttcttcc attttcacat
    22681 cgctgacttc tgtaaattgg ataattgatc gcaatgcttc tagataagcg tatgcttctt
    22741 ctggtgaacg catatcagct tcagaaacga tttcgatcaa aggcgtgcct tgtcggttca
    22801 aatcaacgta agaataaccg ccaataccat gcatgttttt tccagcatct tcttctaaat
    22861 ggacacgttc gatccggatt tttttctttt taccttctac ttcgatttcg atccatccgt
    22921 catgaccgat tggttgatca aattgggaaa tttgatatgc ttttggatta tctgggtaaa
    22981 agtaattttt ccggtcaaaa tgagtatctt tagagatttc acagtttaat gccaaagctg
    23041 ctttcatccc gaattccagc gcagctttat tcattactgg cagcacgcct ggataacccc
    23101 agtcgatcac gtttgtattg ctgtttggtt ctgcaccaaa atgagctggc gctggagaga
    23161 aaatttttga gttggttttt aactcgacgt ggacttctag tccaatgact gtttcaaaat
    23221 tcattcgtct ttccctccta aaatagctgg ttgttgttta tggaattctg ttgcttgttc
    23281 aaacgcatat gccgcttgat acatcgtttt ttcatcaaaa taattaccga tgatctgcaa
    23341 tccaactggt aatccttctg ataaacctgc cggtacagac atacctggaa gtccagccaa
    23401 gttgacaggg atcgttaaga tgtcactcat gtacattgtg attggatcgt taatgttctc
    23461 accgattccg aatgcaactg ttggtgaaga tggcccgatg atcaagtcat aatctgcaaa
    23521 gactttttca aaatctcgtt tgatcaatgt acggacttgt cctgcttttt tgaagtaagc
    23581 gtcataataa cccgcgctta aggagaatgt acctaacatg atacggcgtt ttacttcttc
    23641 accgaatcct tctgaacgtg agttgacata tacatcatct aatgatttca cattttcaga
    23701 gcgataaccg tagcggatac catcgaatcg ttgcaaattg gaacttgctt ctgatgaggc
    23761 aatgatataa taaacagcca caccgtattt tgaatgaggc aagctgactt cttccacaac
    23821 agcacctaat gaacggaatg tttctgctgc tttcaataca gcttctttta cgccaggggc
    23881 cacaccttcg cctaagtatt ctttaggtaa agcaatcttc attcctttga tgtcgcctgt
    23941 cagtccctct gcaaaatcag gaactgaaac gcctgaagaa gtaccgtctt tttcgtcata
    24001 accgctgata gctgtcaatg ctaatgcatt atctttcacc gtacgggtca ttggtccgat
    24061 ttggtcaaga gatgaagcaa aggcaatcaa accaaaacgt gagacacggc cataagtagg
    24121 tttcatccct acgatgccgt taaaagctgc tggttggcgg atacttcccc cagtatcact
    24181 acctaaagaa actggaattt gtccagctgc aactgctgct gcagaaccac cagaagagcc
    24241 accaggtact tttgttgggt cccaagcatt ttttgttttc ttgaaataag aagtttctgt
    24301 acttccaccc atagcaaatt catccatgtt tagcttacca acagggatca tatctgcttg
    24361 atagatttta tccatgaccg ttgcatcata aattggttca aaattgtaaa ggatcttaga
    24421 tgcagcggtt gtcaagatat cttttgttac gatattatcc ttgataccaa ttggaatacc
    24481 agcaaggacg ttgctttcat caatcccttt ttcatctaaa gcttttgcct gttccaatgc
    24541 tttttcttcg ttcaatgtaa tgaatgcctc gacctcaggc tctgtttctt tgattcgttc
    24601 aaatgttgtt tttgtcaaat cttgtacagt gatttctttc gatacaagca tatgatgcag
    24661 ttcttctaga gaacgattgt gtaattctgt cattatgcgc cagcctcccc attatcgatg
    24721 attgcgggta ctttgataaa gccgtcttct gtttccggca catttttcaa taattcatcg
    24781 cggttccatc cagcttgcgc ccgatcttgt cgcatcacgt ttactgtttc aaccacattc
    24841 gacgtaaatg gaacaccttc cgtatctact tcttccagca tttcaaccat atcgatgatt
    24901 ttacctaatt ggtcggtaaa acttgcaagt tcttcatctg cgaacgcaag ttttgctaat
    24961 ttggcaacgt gtttcacttg ttcttcacta atcgccatac taccccttct ttctaaacga
    25021 gttaaaaact ttgctctcta ttttaacaca agagtgccgt gtctgaaatt gtttattgaa
    25081 atttgtctga aaaaactcat ttttcattct tctactgagc gacttttagc caatgcatct
    25141 gccatttgat cttctgtcca aacttcgatt cctaattctt gtgctttggt cagcttgctg
    25201 ccagcatctt ctcctgctac cacgatatcc gtttttttcg atacgctacc agtcactttc
    25261 ccgccaagat tttcgatcgt ctcttttgct tcttcacgag taaatcgtgt tagtttacca
    25321 gtaaggacta ctgttttttc tttaaaagga gactcgactt cttgcaattg ggcatttcga
    25381 agccctttgt attcgaaatt gacgcctgct tgttttaatt cattcatcaa ttcatggact
    25441 tcttcgttac tgaaataagt gactacactg tcggcaatga tatcaccgat cgtatcaagc
    25501 gcaataatcg actcatagtc acttttactc aatgtttcta gatcaccgaa atgttctgct
    25561 aaaatttttg ctgctttcgc tccgacatgt cggattccta acccgaaaat caaccgctcg
    25621 accgaattat ctttgctgtt gtcgattgct gtcaaaatat tatttgctga tttttctttt
    25681 attttgtcca acgtcaataa ctcttcttct gtcaatttgt aaagatcagc aacatctgct
    25741 actagttttt tatcatacat ctgttctagt acgcgcggcc ccaacccatc gatattcatc
    25801 gcatttcttg aaacaaagtg gttcaaacct tctttcattt gagcagggca tttaggattg
    25861 atgcaccgta aggctacttc ttcatccaaa tggacaagtt cacttccaca aactggacaa
    25921 tgtgtaggca attggtattc ttcgctgtct ttaggtcgtt tatctaacac gacttgcgaa
    25981 acttctggga tgatgtctcc cgctttataa atcagaactg tatctttcaa gcggatatcc
    26041 ttcaacttaa tataatcacc gttatgcagg ctggcacggc ttactgtcgt tcctgctact
    26101 cggactggtg tcatgatagc tgttggcgtt actacccctg ttcgaccgac tgtccattcg
    26161 atgttttcga ttagcgtttc aacttcttct ggcggaaact tgtaagctgc tgcccatctc
    26221 ggtgctttga tagtaaaacc aagttgatct tgtagcgaga attcattcac tttgatgacg
    26281 attccatcga tttcataggg caaatccaca cgtttgtcat gatattcttc aatgtatgac
    26341 cacacttcat caatcgaatg acatagacgt ttttcccgat tcgtgtggaa tccaattttt
    26401 tctagctctt ctagcgcatc atactgggtt tttgcttgca taggaccgaa atcagctact
    26461 gtgtataaaa aagtgtccag atttcttttc gctgtgattt tagaatctaa ctgacgcaaa
    26521 gaaccagctg cggcgttccg aggattcgca aagatatctt ttccctctgc ttctctgtct
    26581 tggttcaatt ggacaaatga ccgttttggc atgaaacatt cgcctcttac ttcgatgttc
    26641 attggttctt ttaatttgat tgggaccgac cggacagttt ttaggttttc agtgatgttt
    26701 tctcctacag ttccatcccc gcgagtcgca cctctcacga aatttccatc ttcataacgc
    26761 aaagaaacgg aaagtccatc tatttttagt tcacagcaat agtcaactac acgtcccact
    26821 gctttttgta cacgttgatc aaaagctatc agctcttctt tgctgaaaac gtcgttcaaa
    26881 ctgtaaagcg gaatatcatg agtgactttt tcaaaacctt ctaatacttt tccgcctact
    26941 cgttgagtgg gagaatcaga cgtgatcaaa tcaggatatt gttcctcgat tgctactaat
    27001 tctgcgtatt ctttatcata aacatagtct tccactgttg gtttatcttc tacgtagtat
    27061 tcgcgagacc attggttcag tcttgtacga agttcttctg ctcgtttagc agcttcgtca
    27121 atcgtcattt catgttccat catttaccct ccactactaa gctttttcaa ttggcgcaaa
    27181 ggcagccaaa agacgtttta ctccttgact tgggaaagcc acatctagtt ctagatcctt
    27241 cgacgtcccg cttactcgga cgactgttcc tagaccccat tttttgtgtt tgactttatc
    27301 tccaggtttc cagctgttat tttcccctcc agtagcactt ttagagggaa caaccgctgt
    27361 ctctggctgt ttatagctat atgctgtttt tttagcgaat acaggcgttt ttttaggcgt
    27421 aggacgcatg ccttcgattt ccagtaactc ctgatcgatt tcttctacaa aacgagaagg
    27481 tcggttatat tgcgtccgtc catataatgt tctggagaaa gcatttgtta aatacaacgc
    27541 ttcttctgct cgcgtgatcc ccacatatgc gagacggcgt tcttcctcca gttcgctttc
    27601 ttccatcaat gctcgagata aaggaaaaac cccctcttct agaccaatca ggaatacaac
    27661 tggaaattct aatcctttgg ctgcatgcaa agtcatcaat gtcacttggg aagcatcttc
    27721 ttctaaattg tcaatgtcgg aaacaagtgc cagatcattt aagaagaccg ttagtttttc
    27781 ttctggtgca tcagcgtctt cttcattttg ctgttcgaat tgtttgtcga attcttgtgt
    27841 gaccgttaaa aattcctcca agttttcgat gcgtgcttgt gcttctaatg tattttggat
    27901 ttttaagtct tctagatatc cgctacgatc caaaacctct ttcgtcaatt ctgtcactgt
    27961 caagtatggg atcatctgag tgacttcttg gatcatctct ccaaatgcac ctaattgctg
    28021 tcctgctttt ccaccgatat ttgccagatc cacattttgc gctgcttcaa gcagtggcca
    28081 ttcatgaaga gaagcaaaac tgcgtagttt ttcgatagat cctggtccga tgcctcgttt
    28141 gggcgaatta acgatacgct caaaactgat tgaatcttga ggatttacca gcacattcaa
    28201 atacgctaaa atatctttga tttctttacg atcgtagaac ttgtgccctc caaccatttt
    28261 ataaggaata tttgctttca acagagtttc ttccattaca cgagattgtg cgttcgtccg
    28321 gtacaagatg gcaaagtcgc cgtaattccg atgattgctt cgaatctctt cttgcatccg
    28381 gtctacgata aaacgggttt cgtctcgttc attatctgca cggtagtaag tgatcttgtt
    28441 tccttctttg ttttctgtcc acaagttttt tggttttcga ttgctattgt tttcaatgac
    28501 ttggtttgct gcgctaagga tgtttttcgt ggagcgatag ttttgttcta gcaaaattac
    28561 tgcagcatct ggataatctt tttcaaaatc caagatgttc tgcatatctg caccgcgcca
    28621 gccgtaaata ctttgatctg catctcccac gacgcacagg ttcctaaatc tacctgccaa
    28681 taaattgact agcgtatact gtgcgtgatt tgtatcttgg tactcatcta catgaatata
    28741 atggaatttg ttttgataat acgtgagtga atcaggatgt tcttcaaata atcgaatcgt
    28801 attcatgatc aggtcgtcaa agtccataca ttgattatat tgaagctctt tttggtaagc
    28861 agcataacat tttgccgcaa tctcttcgta ataactgcct tgcatttttg caaaatcctg
    28921 aggggtctgc aggctgtttt ttgcttgaga gattgttcct aatatacttc gaggatcata
    28981 ttttttcgga tcaatgttca gttcttttag tatccgtttc atcaaggtca gctgttcaga
    29041 agaatcgatg attgtaaagt tccggttata cccgataaaa tcaacatctc ttcttaaaat
    29101 acgcacacac atcgagtgaa aagtcgatac ccagacatct tcgcctccag atgcgagaat
    29161 cgcattgaca cgttctttca tttcttttgc agccttattg gtaaaagtaa tcgctaggat
    29221 gttccaagga ttgacttctt tttcctcaat caaataggca atgcggtgtg tcagcactct
    29281 cgtctttcca cttcctgcac ctgccattag cagtaatggt ccgtcagtgt gaagaacagc
    29341 ttctttctga cgggggttca tcccgtttaa taattcagct ttggatgaca ttaacaacat
    29401 cctttctttt atcaatcttg ttaattatag cattttcttg gctttcatta aactagctag
    29461 ttcaaaatca tttgtttctt tttatggatt tttccttgtc aattcgttga tttcgcgcta
    29521 aattaaaaga aagcgaggcg tttgaatgga aaaaaaagga aaaatcaaga aattagataa
    29581 gactcagata tattttattc taaaaggaat atttgttgga ctgctgacag gaatcgtcgt
    29641 tagcctgttt cgattatcta tcgaaaagct aagcgattta gttcgaagta tctatgaaat
    29701 gagtcgggaa cagccgattt acttaattgg cctagctatt tgctgcatcc ttgcagcatt
    29761 tttcgtgggt tatctggtca aaaatgaacc ggatatcaag ggaagcggga ttccacaagt
    29821 agagggacag cttcgcgggg aattatcaat gaactggttc tctgttttat ggaaaaagtt
    29881 cattggcggt gtactttcag ttggtgcggg cctttttcta ggtagagaag gaccttctat
    29941 acagcttggc gcatccattg gacaaggagc tggacaattg tttcggagtc catcttcaga
    30001 agaaaagatt ttaatttcta gcggtgcaag tagcggactt gcagcagcat tcaatgctcc
    30061 tatcgcagga ttactctttg ttttagaaga aatccatcac tccttttctc cacttgtttg
    30121 gctgacatca tttgcttcag caatcactgc taacttcgtc tctctttact ttttcggctt
    30181 gagacctgtt ttatatctag gtgaccttcc ttctttgccg ttgaaatatt atggttcctt
    30241 agtgatctta ggagtacttc taggcatctt aggttttatt tatcagaatg tcttgctggc
    30301 tttgccaaaa ttttacaacc gattgccttt accagcttat tttcacgggt tcgttccttt
    30361 cttgctgatt ttgccaattg gttatttatg gcctagtact ctaggcggag gcaatcaaat
    30421 cgttttagct tttggacatg cttccttgcc aatcattatg attatcggtt tgtttgttct
    30481 tcgattcgtt ttttccatga tttcttacgg tgggggtctt ccagggggga ttttccttcc
    30541 gatattgaca ttgggggctt taatcggtag cttttatgga aacattatgg ttgatttcgg
    30601 gatggatcca atctacatca aggattttgt cgttattgcc atggctggtt atttcacagc
    30661 gattggaaaa gcaccgctga cagcaattat tttagtgaca gaaatggttg gctctcttaa
    30721 tcatctgatg cctttaggat tggctgctct tgttgcttat atcgtcaatg accttctcgg
    30781 cggtaatcca atttacgaat cattattgga acgtctactt ggaaatactc agaccgcctc
    30841 actaaaaggt gcaaggacaa cttttgattt tccagtaacg gcagagagtt ctttagacgg
    30901 attaatggta cgagacttca attggcctaa aaatatgctg ctaatctcta tacgcagagg
    30961 aaatcaggaa atcttgactc acggtgatac gatcatgaat gtaggagata ttttggttat
    31021 cttgactgat gaagcaaact tgccaagaat caagcaggaa atcgaggcta aatcttctat
    31081 cacaaatttg aagcaatcat tttaacgaac atggaaaaac tattttacaa caactattca
    31141 tctacactac ttgattctat atgattctat aaaaaaagaa gaggctggga aaacccagcc
    31201 tcttcttttt tatttctatg cttctgctac tgcaggagct tttctcaata aaccataaat
    31261 cacaccagat acgactgctc cgatagcaat aaacaccaaa tacatgaccg ggtgactcag
    31321 taagaataca acgaaaatcc cgccgtgagg agccagtaat ttgattccga aagctccgac
    31381 taatccgcca gttaaagcag aaccaataat aaaacttgga atggcacgaa tcgggtcagc
    31441 tgctgcaaat ggaatcgatc cttccgtgac aaacgatagt cccatcacga tgttcgtcaa
    31501 accagcatct tgatctgttt tgctgaattt attcttaaac aaacgtgtag caacgaaaat
    31561 tgccaatgga ggtaccattc ctccagccat aacagaagcc atcacgacac ttccgccagt
    31621 tgcaacagtc gtagctaaag ttgctgtacc aaacacataa gcggctttgt tgattggacc
    31681 gcctaaatct gccgccatca ttccagctaa taatgcacca agtaaagcag cattcgaacc
    31741 actcatactt gtcaagaaac cgttcaatgc atcattgatt gctttcatag ggacattgac
    31801 cagcaacatc aagaaaccag taatcagtaa gccaaacact ggataaaaga ggatcgtttt
    31861 aattccatcc aatgattttg gtaagttctt gaataattta cgtaaaaata cgatgacata
    31921 accagctaag aatccaccaa tcagtgcacc taagaatcct gcaccgcctg tattagctaa
    31981 tgcaccagca gcaaatccaa cgatcaaacc agggcggtca ccgatacttg aagcaataaa
    32041 tcctgctaat accggcagca tgaaaccaaa tgcagcttgt ccgatttggt tgaaccagct
    32101 agcagcttga ttgtaattcc ctagatttga taattgatca tgtggcacgc caataaattg
    32161 gtcgatcatg aaagaaagcg caatagcgat tcctccacca ataacgaaag gtaacatgtg
    32221 tgatacgccg ttcatcaaat ctttgtaaat tctttgtccg atagtaccat ccgcatttcc
    32281 ttcttctgaa ctgctttctt ttccatcacc atggaatact ggtgcagtac cactaagggc
    32341 tagattgatc aactcttctg gtttacggat accatcactg acaggacgat tgatcagttc
    32401 ttttccgtca aagcgattca tctctacttt tttgtctgct gcaacgatta caccttctgc
    32461 acgggcgatg tcttcagcag tcaatcggtt tttgattcct tctgaaccgt tcgtttcgac
    32521 tttgatctcc acacccattt ctttcgcttt tttcttcaaa gcatcttctg ccatgtaagt
    32581 gtgagcgatc cctgttggac aagctgtaac ggcaacaacg aattttcttt ctggatgagc
    32641 agaagcttct gcagcttgtg tttcttgttc tgctttttct tctgcttctt tttgctcttc
    32701 agctgtttgg aacaagtctt ggacttcttc tggtgtattt gcttctttta attttcctac
    32761 gaaatctgca tcaatcagca atcgagatag tgctgctaat gcttgcaaat gtgtgtcgtt
    32821 tgcgccttct ggagctgcga tcatgaaaaa cagataagtt ggctgtccgt ctaaggcttc
    32881 ataatctact cctttttggc tttttgcgaa taaaactgtt gcttctttaa ctgcactgtt
    32941 tttcgcgtga ggcatggcga ttccatcacc aagacctgta gaagtttgtg cttcgcgggc
    33001 tagaattcct tctttatatg tttcgatatc ggaaatccgg ccggcatcaa acattttttg
    33061 gaccatttca tcgattgcgc cttttttatc agtcgcttgc aaatccatga tcatcgcttc
    33121 tttgaccatt aaatccttga tattcatgat tatcctcctt gaaactaatc aatttttgtg
    33181 atttcaactt ctggcagcaa ctcatcaatc aattcgcgag ctgccaagtc atcagaaaaa
    33241 gctgttgcgc ttccgcaagc aactccccat ttaaaggctt cgattggatc tttcgtacgg
    33301 ctgtaagccc caataaagcc agcaatcatt gaatcccctg cacctactga atttttcaat
    33361 gggcgaacta atacattcgt tttataagtt ccttgttccg tgaagagcaa agcaccgtct
    33421 ccagccattg agatcagcac attctgtgcc ccttcagcta gtaatttttt cccatacgga
    33481 atgatttctt ctacagattg gaaggttgtg ttgtaaagct cagctaattc gtgattgttt
    33541 ggtttgatca acaacggttt cttaacaagt gcatttttta ggtcatcacc tgttgtatca
    33601 atcacgaaat ctgcaccttt ttctcgaatg atatcaatca gttcttgata aaatcctttt
    33661 cgcaggctag ctggcgcgct tcctgacaat acgacgatat ctccttgtcc tactgttcct
    33721 aatgcttgct tcaactcatc gatttcttca tctgaaatag atgggcctaa gccattgatc
    33781 tcagtttcag tgtctgattt tagtttgata ttgattcttg tatcttcttt tacggttgtg
    33841 aacaaagact ggatttgttc tttttcaagc cacttcgaaa taaattcgcc agtgaaccct
    33901 cctaaaaacc ctagtgctgt agattgagaa tcgatccgtt tcaaaatacg ggaaacattg
    33961 atcccttttc ctccaggtaa cttgaagtct tccttcattc ggttcaaatc gcctagtttt
    34021 aacccatcta cgcgtacgat ataatcaatt gatggattaa gtgttactgt gtaaatcatt
    34081 gtattgcctc cttaattgtt gtcttttcac ggaattcatt aacgaattct aatggacaac
    34141 gctcggttat gatcgtggct gcttccaaag gtgcaacaga ggtgaaggac aactggttga
    34201 atttggtatg atcaatcaat acaaatgtct cttcggaatt tttcatggct aaacgtttaa
    34261 cagctgcctc ttctggatcc ggagtcgtga atccttgttc aagatgggca ccgttcattc
    34321 ccataaatgc tttattaaaa tgaaaatttt ttagttgttc tatcgcatta gatcctaaaa
    34381 cagcattagt tgccagcttt atcgttccac ctaaaataat ggtagcaagc tccaaatcaa
    34441 ctaaatttgc cgcatgttga acggaatttg tcactactgt gatctgtttt ccttgcagaa
    34501 aaggaatcat ttctaacgta gtagagccag cgtctaaata aatgacatct tcgtcattaa
    34561 tggtttcagc tgccgatttg gcgatttgct gtttttcgtg aacgtttttg agtgattttt
    34621 cactcatatt ctgctcaaaa ttcaataatt gctctttctt agctccgcca tgaactcttt
    34681 ctagaaaccc actattctcc agctcttgta aatcacgtct tatcgttgat tcagaagcat
    34741 tcaaaaggtt tacaagatct tgtgatttca cgattttctg ctgttcaagc aagtctagga
    34801 ttttttgtct gcgttcctct gtaagcattt tcacgatcac ctcaatacaa ataatagcat
    34861 attttt
//
LOCUS       DACDSY010000009        34467 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDSY010000009 DACDSY010000000
VERSION     DACDSY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.009"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            212..604
                     /locus_tag="IWK44_000418"
     CDS             212..604
                     /locus_tag="IWK44_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9548974.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IWK44_000419"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IWK44_000419"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP9548975.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="IWK44_000420"
     CDS             3165..4775
                     /locus_tag="IWK44_000420"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP9548976.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="IWK44_000421"
     CDS             5067..5936
                     /locus_tag="IWK44_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP9548977.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="IWK44_000422"
     CDS             6101..7372
                     /locus_tag="IWK44_000422"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9548978.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="IWK44_000423"
     CDS             7369..8664
                     /locus_tag="IWK44_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP9548979.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="IWK44_000424"
     CDS             8763..9026
                     /locus_tag="IWK44_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP9548980.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="IWK44_000425"
     CDS             9471..9920
                     /locus_tag="IWK44_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9548981.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="IWK44_000426"
     CDS             9977..11395
                     /locus_tag="IWK44_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9548982.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="IWK44_000427"
     CDS             11395..12255
                     /locus_tag="IWK44_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP9548983.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="IWK44_000428"
     CDS             12429..13202
                     /locus_tag="IWK44_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9548984.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="IWK44_000429"
     CDS             13349..13714
                     /locus_tag="IWK44_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP9548985.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="IWK44_000430"
     CDS             complement(13862..15070)
                     /locus_tag="IWK44_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9548986.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="IWK44_000431"
     CDS             complement(15089..15367)
                     /locus_tag="IWK44_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548987.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="IWK44_000432"
     CDS             15560..16216
                     /locus_tag="IWK44_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9548988.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="IWK44_000433"
     CDS             complement(16658..17455)
                     /locus_tag="IWK44_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP9548989.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="IWK44_000434"
     CDS             complement(17452..18636)
                     /locus_tag="IWK44_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9548990.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="IWK44_000435"
     CDS             complement(18661..19908)
                     /locus_tag="IWK44_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP9548991.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="IWK44_000436"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="IWK44_000436"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP9548992.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="IWK44_000437"
     CDS             21888..22202
                     /locus_tag="IWK44_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP9548993.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="IWK44_000438"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="IWK44_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP9548994.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="IWK44_000439"
     CDS             22887..23531
                     /locus_tag="IWK44_000439"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP9548995.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="IWK44_000440"
     CDS             23546..23875
                     /locus_tag="IWK44_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548996.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="IWK44_000441"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="IWK44_000441"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP9548997.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="IWK44_000442"
     CDS             24863..25687
                     /locus_tag="IWK44_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP9548998.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="IWK44_000443"
     CDS             25680..26027
                     /locus_tag="IWK44_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP9548999.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="IWK44_000444"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="IWK44_000444"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP9549000.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="IWK44_000445"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="IWK44_000445"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP9549001.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWK44_000446"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWK44_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP9549002.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWK44_000447"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWK44_000447"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP9549003.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="IWK44_000448"
     CDS             31684..32451
                     /locus_tag="IWK44_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9549004.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..>34467
                     /locus_tag="IWK44_000449"
     CDS             32441..>34467
                     /locus_tag="IWK44_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9549005.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCR"
BASE COUNT        11074 a         6117 c         7239 g        10037 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcg
//
LOCUS       DACDSY010000010        33956 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDSY010000010 DACDSY010000000
VERSION     DACDSY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.010"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(87..668)
                     /locus_tag="IWK44_000450"
     CDS             complement(87..668)
                     /locus_tag="IWK44_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9549006.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(801..1484)
                     /gene="rpiA"
                     /locus_tag="IWK44_000451"
     CDS             complement(801..1484)
                     /gene="rpiA"
                     /locus_tag="IWK44_000451"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP9549007.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            1803..2216
                     /gene="rpsL"
                     /locus_tag="IWK44_000452"
     CDS             1803..2216
                     /gene="rpsL"
                     /locus_tag="IWK44_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP9549008.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            2300..2770
                     /gene="rpsG"
                     /locus_tag="IWK44_000453"
     CDS             2300..2770
                     /gene="rpsG"
                     /locus_tag="IWK44_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP9549009.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            2864..4951
                     /gene="fusA"
                     /locus_tag="IWK44_000454"
     CDS             2864..4951
                     /gene="fusA"
                     /locus_tag="IWK44_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9549010.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5123..6310
                     /gene="tuf"
                     /locus_tag="IWK44_000455"
     CDS             5123..6310
                     /gene="tuf"
                     /locus_tag="IWK44_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9549011.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            6717..7025
                     /gene="rpsJ"
                     /locus_tag="IWK44_000456"
     CDS             6717..7025
                     /gene="rpsJ"
                     /locus_tag="IWK44_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP9549012.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7056..7685
                     /gene="rplC"
                     /locus_tag="IWK44_000457"
     CDS             7056..7685
                     /gene="rplC"
                     /locus_tag="IWK44_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP9549013.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            7713..8336
                     /gene="rplD"
                     /locus_tag="IWK44_000458"
     CDS             7713..8336
                     /gene="rplD"
                     /locus_tag="IWK44_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP9549014.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            8336..8626
                     /gene="rplW"
                     /locus_tag="IWK44_000459"
     CDS             8336..8626
                     /gene="rplW"
                     /locus_tag="IWK44_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP9549015.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            8665..9498
                     /gene="rplB"
                     /locus_tag="IWK44_000460"
     CDS             8665..9498
                     /gene="rplB"
                     /locus_tag="IWK44_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP9549016.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            9541..9819
                     /gene="rpsS"
                     /locus_tag="IWK44_000461"
     CDS             9541..9819
                     /gene="rpsS"
                     /locus_tag="IWK44_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP9549017.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            9842..10189
                     /gene="rplV"
                     /locus_tag="IWK44_000462"
     CDS             9842..10189
                     /gene="rplV"
                     /locus_tag="IWK44_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP9549018.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            10203..10859
                     /gene="rpsC"
                     /locus_tag="IWK44_000463"
     CDS             10203..10859
                     /gene="rpsC"
                     /locus_tag="IWK44_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP9549019.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            10862..11296
                     /gene="rplP"
                     /locus_tag="IWK44_000464"
     CDS             10862..11296
                     /gene="rplP"
                     /locus_tag="IWK44_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP9549020.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            11286..11474
                     /gene="rpmC"
                     /locus_tag="IWK44_000465"
     CDS             11286..11474
                     /gene="rpmC"
                     /locus_tag="IWK44_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP9549021.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            11499..11765
                     /gene="rpsQ"
                     /locus_tag="IWK44_000466"
     CDS             11499..11765
                     /gene="rpsQ"
                     /locus_tag="IWK44_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP9549022.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            11823..12191
                     /gene="rplN"
                     /locus_tag="IWK44_000467"
     CDS             11823..12191
                     /gene="rplN"
                     /locus_tag="IWK44_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP9549023.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            12228..12536
                     /gene="rplX"
                     /locus_tag="IWK44_000468"
     CDS             12228..12536
                     /gene="rplX"
                     /locus_tag="IWK44_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP9549024.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            12564..13103
                     /gene="rplE"
                     /locus_tag="IWK44_000469"
     CDS             12564..13103
                     /gene="rplE"
                     /locus_tag="IWK44_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP9549025.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13122..13307
                     /locus_tag="IWK44_000470"
     CDS             13122..13307
                     /locus_tag="IWK44_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP9549026.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            13343..13741
                     /gene="rpsH"
                     /locus_tag="IWK44_000471"
     CDS             13343..13741
                     /gene="rpsH"
                     /locus_tag="IWK44_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP9549027.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            13773..14309
                     /gene="rplF"
                     /locus_tag="IWK44_000472"
     CDS             13773..14309
                     /gene="rplF"
                     /locus_tag="IWK44_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP9549028.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            14470..14826
                     /gene="rplR"
                     /locus_tag="IWK44_000473"
     CDS             14470..14826
                     /gene="rplR"
                     /locus_tag="IWK44_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP9549029.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            14847..15347
                     /gene="rpsE"
                     /locus_tag="IWK44_000474"
     CDS             14847..15347
                     /gene="rpsE"
                     /locus_tag="IWK44_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP9549030.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            15362..15541
                     /gene="rpmD"
                     /locus_tag="IWK44_000475"
     CDS             15362..15541
                     /gene="rpmD"
                     /locus_tag="IWK44_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP9549031.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            15585..16025
                     /gene="rplO"
                     /locus_tag="IWK44_000476"
     CDS             15585..16025
                     /gene="rplO"
                     /locus_tag="IWK44_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP9549032.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16025..17320
                     /gene="secY"
                     /locus_tag="IWK44_000477"
     CDS             16025..17320
                     /gene="secY"
                     /locus_tag="IWK44_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP9549033.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            17382..18029
                     /locus_tag="IWK44_000478"
     CDS             17382..18029
                     /locus_tag="IWK44_000478"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP9549034.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            18220..18438
                     /gene="infA"
                     /locus_tag="IWK44_000479"
     CDS             18220..18438
                     /gene="infA"
                     /locus_tag="IWK44_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP9549035.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            18471..18587
                     /gene="rpmJ"
                     /locus_tag="IWK44_000480"
     CDS             18471..18587
                     /gene="rpmJ"
                     /locus_tag="IWK44_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP9549036.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            18605..18970
                     /gene="rpsM"
                     /locus_tag="IWK44_000481"
     CDS             18605..18970
                     /gene="rpsM"
                     /locus_tag="IWK44_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP9549037.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            18998..19387
                     /gene="rpsK"
                     /locus_tag="IWK44_000482"
     CDS             18998..19387
                     /gene="rpsK"
                     /locus_tag="IWK44_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP9549038.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            19467..20405
                     /locus_tag="IWK44_000483"
     CDS             19467..20405
                     /locus_tag="IWK44_000483"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP9549039.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            20433..20813
                     /gene="rplQ"
                     /locus_tag="IWK44_000484"
     CDS             20433..20813
                     /gene="rplQ"
                     /locus_tag="IWK44_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP9549040.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21085..21924
                     /locus_tag="IWK44_000485"
     CDS             21085..21924
                     /locus_tag="IWK44_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9549041.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            21900..22769
                     /locus_tag="IWK44_000486"
     CDS             21900..22769
                     /locus_tag="IWK44_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9549042.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            22759..23556
                     /locus_tag="IWK44_000487"
     CDS             22759..23556
                     /locus_tag="IWK44_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9549043.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            23759..24505
                     /gene="truA"
                     /locus_tag="IWK44_000488"
     CDS             23759..24505
                     /gene="truA"
                     /locus_tag="IWK44_000488"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9549044.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(24838..27507)
                     /locus_tag="IWK44_000489"
     CDS             complement(24838..27507)
                     /locus_tag="IWK44_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9549045.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(27745..27933)
                     /locus_tag="IWK44_000490"
     CDS             complement(27745..27933)
                     /locus_tag="IWK44_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP9549046.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            28203..28565
                     /locus_tag="IWK44_000491"
     CDS             28203..28565
                     /locus_tag="IWK44_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9549047.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            28617..30299
                     /locus_tag="IWK44_000492"
     CDS             28617..30299
                     /locus_tag="IWK44_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP9549048.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            30416..32452
                     /gene="recG"
                     /locus_tag="IWK44_000493"
     CDS             30416..32452
                     /gene="recG"
                     /locus_tag="IWK44_000493"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP9549049.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            32501..33502
                     /gene="plsX"
                     /locus_tag="IWK44_000494"
     CDS             32501..33502
                     /gene="plsX"
                     /locus_tag="IWK44_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP9549050.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            33624..33866
                     /gene="acpP"
                     /locus_tag="IWK44_000495"
     CDS             33624..33866
                     /gene="acpP"
                     /locus_tag="IWK44_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9549051.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        11136 a         6237 c         7316 g         9267 t
ORIGIN      
        1 cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa acgaacagtg
       61 cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc tttttcaggt
      121 aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga aataaattgg
      181 atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt tgcatcgatt
      241 tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac taatgtacgt
      301 tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa atagattttt
      361 cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc gcgatctttg
      421 aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg aagttggcaa
      481 atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg aacagcagta
      541 gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc aaagtcagat
      601 aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc ataaccaata
      661 attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca gtaaaaatgt
      721 agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct ttttttattg
      781 gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc aacgcctttt
      841 tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc tactacccca
      901 acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc tagatgcaga
      961 tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc tgtacgcaag
     1021 acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc ataagggatc
     1081 acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga atcaacgatc
     1141 caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc agcgccgccg
     1201 cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt caaatcgact
     1261 acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg ttcttttgtc
     1321 gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg gcgaccaatc
     1381 tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat cccgtccttt
     1441 acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc gtccaccttt
     1501 ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt aaaaagatac
     1561 cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg ttgacatgtt
     1621 ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg aagaaacgtg
     1681 ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat tttttatcac
     1741 aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg aggaaacaag
     1801 gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg gaaaaatcta
     1861 attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact aacgtaaact
     1921 cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa aaaccgaact
     1981 cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt actgcttata
     2041 tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc ggtggtcgtg
     2101 taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat actgctggtg
     2161 ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa gcttaatttt
     2221 aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat gaacaatatt
     2281 tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg atgttttacc
     2341 agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa tggttgacgg
     2401 aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca aagaatctac
     2461 aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac ctgttcttga
     2521 agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag ttcgccctga
     2581 acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc gcggtgaaca
     2641 cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca caggtgcttc
     2701 tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt ttgctcacta
     2761 tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag atagtgtcct
     2821 tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca gagaattttc
     2881 tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta aaacgactgc
     2941 tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa ctcatgaagg
     3001 agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga tcacttctgc
     3061 tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac caggacacgt
     3121 agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg taactgttct
     3181 tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag caacaactta
     3241 tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg acttcttata
     3301 ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc aattaccaat
     3361 tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg ctgaaatgta
     3421 tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat accgcgaatt
     3481 agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg aagaacttac
     3541 tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag gtatccgccg
     3601 cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta agaacaaagg
     3661 ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag atatcccagc
     3721 tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag acgacagcgc
     3781 tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc gtttaacttt
     3841 cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg caacaaaagg
     3901 caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt cagaaatctc
     3961 tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa ctactgggga
     4021 cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc cagaaccagt
     4081 tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg gtgtggcttt
     4141 acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg aaacaggcga
     4201 aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc gtatgcgtcg
     4261 tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg aaactttccg
     4321 tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag gtcaatacgg
     4381 tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat ttgaaaatgc
     4441 gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg gtttgaaaga
     4501 tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag ctaaacttta
     4561 cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg ctgcttctat
     4621 ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa tcatggctgt
     4681 agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa ctgctcgtcg
     4741 tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc gtgcgatggt
     4801 tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc aaggtcgtgg
     4861 tacatttaca atgacatttg accactacga agatgtacca aaatcagtac aagaagaaat
     4921 cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt cctgcaaaga
     4981 gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta tcgatgatat
     5041 gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa aatatattgt
     5101 atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta aaccacacgt
     5161 taacattggt actatcggac acgttgacca tggtaaaact acattaacag ctgcaatcac
     5221 aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa tcgatggtgc
     5281 tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt atgaaacaga
     5341 tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta aaaacatgat
     5401 cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg acggcccaat
     5461 gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt acatcgttgt
     5521 attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag ttgaaatgga
     5581 agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg tagttgctgg
     5641 atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc ttgaattaat
     5701 ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac cattcatgat
     5761 gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag gtcgtgttga
     5821 acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg aagaaacttc
     5881 aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg ctgaagctgg
     5941 agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac gtggacaagt
     6001 tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag tatacgtgtt
     6061 gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc cacaattcta
     6121 cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg aaatggttat
     6181 gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta tcgaaaacgg
     6241 tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg ttacagaaat
     6301 caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg taatcgctcg
     6361 gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga tcaggaaaaa
     6421 agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt ttctaaatga
     6481 aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga gtttatatat
     6541 attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc agaatgattt
     6601 cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga acgagcgtga
     6661 cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg aacaagatgg
     6721 caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta gatcaatcag
     6781 cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt ccaattccat
     6841 taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac aaagattcac
     6901 gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac ccaacaccaa
     6961 aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc gaaatcaaat
     7021 tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc ttagggaaaa
     7081 aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta actgttgtag
     7141 aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc tacgaagcta
     7201 ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg aaaggtcatg
     7261 ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat gttgagctag
     7321 gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga gacattattg
     7381 atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc cacggacaaa
     7441 gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca atgggtccag
     7501 ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt ggcaaccgcg
     7561 taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt atcttgatca
     7621 aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct gtgaaagcta
     7681 aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta ttcaaacaag
     7741 atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt gaacctaatg
     7801 aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa ggaacacacg
     7861 cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt caaaaaggaa
     7921 caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt ggcgtagtct
     7981 ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt cgtttagcaa
     8041 tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc gaaggcttga
     8101 acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta tctatcgact
     8161 ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca gcacgtaact
     8221 taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt gtatcaaata
     8281 caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt gcataatgaa
     8341 cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg ccatggatga
     8401 caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa aacaagcggt
     8461 agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac gtgcgaaatt
     8521 caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga tcgttacatt
     8581 gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac taatagataa
     8641 ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac ctcaaatggt
     8701 cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc tgaaaaatca
     8761 ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat cactgtacgt
     8821 catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg taacaaagat
     8881 aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc taacatcgcg
     8941 ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg actagaagtg
     9001 ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc tcttccatta
     9061 gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg taaaggtgga
     9121 caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg caaatacgta
     9181 ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg tgctacaatc
     9241 ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg acgctctcgt
     9301 tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga tcacccacac
     9361 ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc ttggggtcaa
     9421 ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact tattgttcgt
     9481 cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag gaggttcacc
     9541 atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa aaaagttgaa
     9601 gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg ttcaacagtt
     9661 ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca tgtaccagtt
     9721 tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac aagaacttat
     9781 cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag gataaatcca
     9841 aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca cttcacctcg
     9901 aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg caatttcaat
     9961 cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga tgtcagcagt
    10021 tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa gcgaagcttt
    10081 tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat cagcttctcc
    10141 aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag gagggataat
    10201 cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc gactgggatg
    10261 ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta agaatccgta
    10321 aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc gaacgcgcaa
    10381 caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt ggtaaaggcg
    10441 gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa agaatccata
    10501 tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt gaaggaatcg
    10561 cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc atccaacgct
    10621 caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta aacggagcag
    10681 atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact ttacgtgcgg
    10741 acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga gttaaagtgt
    10801 ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga gggaaataat
    10861 catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa tgcgcgggga
    10921 agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg ttgattcaca
    10981 ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt acatgaaacg
    11041 tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca aagcaattgg
    11101 ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag taaaacgtgg
    11161 taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag cgttacgtct
    11221 agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa tgggtggtga
    11281 atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt gatcaagaaa
    11341 aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt caattagaaa
    11401 acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact gtgttgcgtg
    11461 aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga agaaatcaac
    11521 gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt accgttgctg
    11581 tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct aagaaataca
    11641 aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt atggaaactc
    11701 gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa gcagttatta
    11761 tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg aggatacaca
    11821 acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca cgtgaaattt
    11881 tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt gatgtgatcg
    11941 ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac gtcgttaaag
    12001 ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat attaaatttg
    12061 atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca cgtatctttg
    12121 ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca ctagcaccag
    12181 aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg tttgttaaaa
    12241 aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc gttgtactag
    12301 cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt aaaaaacacc
    12361 aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg ccaattcatg
    12421 tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc tacaaagaag
    12481 tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat aaataaaagt
    12541 ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt aaagaagtaa
    12601 ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct aaagttgaaa
    12661 aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac ttagataaag
    12721 cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa gctaaaaaat
    12781 caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt acattgcgcg
    12841 gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca cgtgtacgtg
    12901 acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact ttaggtatta
    12961 aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta cgtggtatgg
    13021 acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg ttgacacaat
    13081 taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa aaatcaatga
    13141 ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt tgcgaacgtt
    13201 gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc ttccgcgaac
    13261 ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa gtaaattcgc
    13321 ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt ttctaactcg
    13381 tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat caaaaattaa
    13441 acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg aatacatcga
    13501 agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg gcgaacgtgt
    13561 tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca aagctgacga
    13621 agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg aaggtgttgt
    13681 cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt acgtatggta
    13741 atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat aaagtcgttg
    13801 ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt aaaggaccaa
    13861 aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa ggaaacgaag
    13921 taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga acaactcgtg
    13981 cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg ttagaactaa
    14041 tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac gttggttact
    14101 cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca gctaacacac
    14161 aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca aacattcgtg
    14221 gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc gaatatgtac
    14281 gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag aagccagcag
    14341 caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta cttgtgccgc
    14401 agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa aaataaagag
    14461 gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag acatcgtcgt
    14521 gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt tcgttctaac
    14581 aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc aagtgcctct
    14641 accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc tgtcggtaaa
    14701 ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga ccgtggtgga
    14761 tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa tggactagaa
    14821 ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat ttggaattag
    14881 aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga cgtcgtctac
    14941 gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt ggtacaggta
    15001 aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag aaaaacttag
    15061 ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct tttagtggtg
    15121 gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt ggaccagttc
    15181 gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta ggctcaaata
    15241 caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa cgtgcagaag
    15301 aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga ggacaacaat
    15361 aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc aaaaccaacg
    15421 cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta agccttctaa
    15481 tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg aagaaattta
    15541 atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa cttcatgaat
    15601 taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt acttcatctg
    15661 gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca ggcggcggtg
    15721 tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca aaacgaggat
    15781 tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg aaccgctttg
    15841 aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg aaaaacgaaa
    15901 aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact gtgaaagcag
    15961 caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca atcgaggtga
    16021 tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat cgaaaatcct
    16081 atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg taccaggcgt
    16141 aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt tgaacttggt
    16201 aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac catacattac
    16261 tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg ttgaatggtc
    16321 gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc tgacattagt
    16381 attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg ctcaattagg
    16441 tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc taacagccgg
    16501 tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg gaaatggcgt
    16561 atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca gagaaattta
    16621 tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta tttttgtggc
    16681 cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc aacaagctga
    16741 acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa gcagctatct
    16801 gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt cgtttattgc
    16861 tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa actggtacaa
    16921 agtgatgatg gagatcttta actataatac agcacctggt gctacgattt atacgattct
    16981 gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga aattagcgga
    17041 aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg ggactgaaga
    17101 gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc ttggtttagt
    17161 ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa tcggattagg
    17221 cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac agttagaagg
    17281 cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt tgaggtttcc
    17341 cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc attttaatgg
    17401 ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct tatggtattc
    17461 cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca gctcttggcc
    17521 tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta acaaatggaa
    17581 tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta gatggctttc
    17641 cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg aataaaaaaa
    17701 ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt ctagccggcc
    17761 gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct acaaaagttg
    17821 aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat gataaacctg
    17881 agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc ttggcttatt
    17941 ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat gctgtatttg
    18001 cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact tgcattatgc
    18061 agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac ttctagagaa
    18121 tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc gccattttat
    18181 cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga agatatgatt
    18241 gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt tgaactagaa
    18301 aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta catccgtatc
    18361 ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg tggccgtatt
    18421 acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc atgaaagtaa
    18481 gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa ggacgcgtta
    18541 tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg gaggtgctat
    18601 aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag tagtttcact
    18661 tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg atgctggtgt
    18721 atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta tccgtgcaga
    18781 agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga acatcaaacg
    18841 tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc ctgttcgtgg
    18901 acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta tcgctggcaa
    18961 gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa aagtgaatcg
    19021 taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt ctacattcaa
    19081 taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt catcagctgg
    19141 ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa tggcagcaga
    19201 aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa cagttaaagg
    19261 acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt tagaagtgac
    19321 tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa aacgccgtcg
    19381 tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac aagacacttt
    19441 tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga atcgcaaaaa
    19501 ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga gggtatggga
    19561 ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt gctgccatca
    19621 ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt gttagagaag
    19681 acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat ggccaagaag
    19741 aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc gatattatcg
    19801 ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc tctgaaggag
    19861 ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa gcagatgaaa
    19921 ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac acacctgttc
    19981 gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat tttgataaat
    20041 tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg agtttgtctg
    20101 cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa gcaaaaaatg
    20161 ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa atgacgatcg
    20221 aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga atcaatactg
    20281 tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac ttaggacgta
    20341 aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta cgtaaagaag
    20401 attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta ggacgcacat
    20461 ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc aacgaacgta
    20521 ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa atgatcactt
    20581 taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta cgtaatgaag
    20641 ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta caaaaactgt
    20701 tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc attttgaaga
    20761 cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc taaaatcaca
    20821 ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga agatattttt
    20881 ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa gcactcatca
    20941 taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga aggcataaaa
    21001 aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg ttagtaggaa
    21061 ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa gataaattat
    21121 aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac aattgataaa
    21181 ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact tgccaaaacg
    21241 atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca aatattggat
    21301 gaagaaaata tttggacgat ccgacagatg gttggaatga tttttcaaaa tccagacaat
    21361 cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa tcaagggatt
    21421 cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag aatggctgag
    21481 tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt tgccattgcc
    21541 ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag tatgctagat
    21601 ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga aagtcaattg
    21661 accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg gatactagtg
    21721 atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc tgctggtcct
    21781 gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag cgcactgaaa
    21841 gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt ggattggtta
    21901 tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca tttgaacaaa
    21961 gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca ttagtagggc
    22021 ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt aagccaacaa
    22081 gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat aagaatttaa
    22141 agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag ctattcgaag
    22201 aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc gaagaagaag
    22261 cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc tatttggaac
    22321 gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct ggtgtgcttg
    22381 ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat ccccagggca
    22441 gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc actattgtat
    22501 tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat gtgttggaaa
    22561 agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt gaatggctaa
    22621 aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta atggctaaag
    22681 ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct atcgtccatt
    22741 atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat gcctggagat
    22801 tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta ttttatcgga
    22861 atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt cacattactt
    22921 tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg cccattgatc
    22981 tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg agaaacttat
    23041 tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct ctttattttc
    23101 tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac accgcctttg
    23161 gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg gttcccagtc
    23221 cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt gatggacgaa
    23281 acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga aggaaaccta
    23341 gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag cagcttcaac
    23401 cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg ggaaggaaga
    23461 acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat tgcttttgta
    23521 ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc gctatctatt
    23581 cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa gaaatattag
    23641 ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa agaacgaaaa
    23701 aattttatag aaaaatcatt attccagata accttttagg aacaaaggag agaaaaacat
    23761 ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat tccaaaaaca
    23821 gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa tggcaaatgg
    23881 aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg cgatcgggca
    23941 agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt tcgcgctaga
    24001 tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag aagacttcca
    24061 cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag ggaaaccacg
    24121 cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt tggaaaaaat
    24181 tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt gtgcatcagg
    24241 aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg taaatgaaac
    24301 aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga tgatacggat
    24361 cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata gtatcccaga
    24421 tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg aaggacttta
    24481 tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct tcaagaaata
    24541 agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt attttcgaag
    24601 aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt aactctgagt
    24661 cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg acttgtggac
    24721 gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc aaaaaacgat
    24781 ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat cgtttcttta
    24841 ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg gatcaatcgt
    24901 gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca atgcaataca
    24961 taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga aagtgacagt
    25021 atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc tgaccttgac
    25081 tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg tcaccaacat
    25141 ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt ccatttcgac
    25201 acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta gaccaacgac
    25261 tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa atgtcactgt
    25321 tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga tttgcgtttt
    25381 tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg caaaattggt
    25441 tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc aagtgagctc
    25501 ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct ttgtgatcgt
    25561 cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt catttagacc
    25621 agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg gctgctcaac
    25681 atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat tctgatacag
    25741 tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg tgctttcgtt
    25801 tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat tattgttata
    25861 acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt aagaggaaga
    25921 attacgattt cttatagaag aaaacaaact gataccactg taaccatagt tgatcgaatc
    25981 gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat ctttttttgt
    26041 ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc gggaagcata
    26101 attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg cttcagcaga
    26161 catgatcaat aataacaata tactcagatg attgaatgaa agttttttat gctgataaaa
    26221 tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg aagtcatcgt
    26281 cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga gagcagctac
    26341 gaccaaaatg attcctaaca acagcccacc atcgtcttgt tctatctttt cccatccttt
    26401 tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata aaaacatatt
    26461 cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac ttgcgataag
    26521 tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc gactggcaaa
    26581 ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa tcgatccgta
    26641 ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat aagaagtcgc
    26701 ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa gatccagcac
    26761 tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga taccataagg
    26821 aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa aatataagaa
    26881 agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga gtaaataact
    26941 gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca aaatcactaa
    27001 tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag tgataaatga
    27061 gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct tgaacgtacg
    27121 tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca gaacatacag
    27181 tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga tcccgcctag
    27241 atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg tataaaaaat
    27301 actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa actgtgaaaa
    27361 ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac ttagataaac
    27421 aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc cgttttctct
    27481 aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta tgaagttgta
    27541 ttcagtttat catatccttg tttttctctg cttaaaaaga gtctagtttt cacaaaaaaa
    27601 taaaatttag aataaataac ttcataattt gttcgtataa caaaaataaa cgcaaaaaaa
    27661 gggtaggaat caaaagtccc acccttaaaa atttttatta aataaaaaac cgttcactat
    27721 ctgaacggtc caagtatcat tcaattaaac gcgttcaact tttcctgatt tcaaagcacg
    27781 agttgacacc caaacttttt taggttttcc gtcgaccata acgcgaactt tttgtaagtt
    27841 aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc cactttttgt
    27901 cttgcgacct gtaaagtaac aaacttttgc catggtaatt tcctcctttg ccttgatttg
    27961 gagcaataga ataattcagc tcataccaga ctaacatatc atatagaatg ggaatttgca
    28021 agtacatttt catagataca attgacaatt gagaagaata attgtaaatt aaacaaggtt
    28081 atacctgaaa ttttgcaagg tttttgagta ctttgggtaa ctgcctttta tttcaagttg
    28141 cgctatgata aaatatgata gaagaaaaat gggagttttt tacccgttaa ggaggctcat
    28201 tcatggctgt aaaaattcaa acgtcagctg gaaccattga aattaccaat gatgtaattg
    28261 caacagttgt tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg gccagcaaaa
    28321 atcaaattaa agataatatt actgagattt tgcgtaaaga aaattactca agaggagtag
    28381 ttgtccgcca agaagataat ggtattgctg tagatgttta tacgattatc agctatggta
    28441 cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat ctagaggcat
    28501 tattaggcgt aactgccaat tcagttaatg tctttgtaca aggtgttcgt gtactgccag
    28561 actaggcagg agactggctg gtaactcagc agtcgatctg aaggaggatt atctaggtga
    28621 gtgtaacaga aataagtgca agccagttcc aggaaatggt ccaggcaggt gcgaatcgtc
    28681 tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt ccctgtgcca gatggcgata
    28741 cgggaacaaa tatgaatcta tctatgacta gtggagctaa ggcagtagta gattcaacct
    28801 cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg attgctgatg ggtgcgcgcg
    28861 gaaattccgg cgttattttg tctcaattgt ttcgtggatt ttctaagcaa attccagatg
    28921 ttgtctcatt aaatgcttct gatcttgctg cagccttcac tcatggagtt gaaactgcgt
    28981 ataaagcagt aatgaaacca gtagaaggaa cgattttgac agtagctaga gaagcagcga
    29041 aagctggaga aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg acagctgttg
    29101 taaaaggagc aaagcgtgct ttagctaaaa ctccagactt gcttccagtc ctaaaagaag
    29161 tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt ttatgagggc tttttaagtg
    29221 cattgaatgg agaattccaa gcggatgaaa catatgaacc ttctccagca gaaatggatg
    29281 atatggtcaa tgcagaacat catcgcagtg tccaaggtca aatggcgaca gaagatatcc
    29341 attttggtta ctgtacagaa atcatggtaa aaatcggaga aggacctaca gtcgatagta
    29401 cattcgatta cgaagaattc cgtaactatt tagatggtat aggagattct ttacttgtgg
    29461 tcaatgatga cgagatcatc aaagtccacg tgcatacaga aaacccagga gaagtcttga
    29521 attatggaca aaaattcggt tcattagtca aaatcaaagt cgacaacatg cgcttgcagc
    29581 atgagacgat tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa gaaataccac
    29641 gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag ctgtttgaaa
    29701 gtttaggcgc taattatgtt atcagtggtg gacaaacaat gaatccaagt acggaagaca
    29761 ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca aacaataaga
    29821 acatcttcat ggcagctgat caagcagcag aagtagcaga tatcccagta gcagttgttc
    29881 cttcaagaac gatttcccaa ggattgacag ctatgctttc attcaatgat caagcttctt
    29941 tagaagaaaa caaagaaacg atgacagaaa tgttgtctag cgttgtgagc ggtcaagtca
    30001 ctcacgcgat cagagatacc tcaattgacg gtgtagagat caaagaagga gactttttgg
    30061 gcatgatcga tggaaaaatc ctgatctcta acccagatat cacagcaaca gcgttaagta
    30121 cactagaaca aatgatcaat gaggacacag agatcgtaac gatcttggca ggtgatgaag
    30181 gaacagttga acaagcaaat gaatttgctg agaaactaac agaaaaacat cctgatttgg
    30241 aaatcgagat ccaccaagga gatcagcctg tctatcctta tctattttct gctgaataat
    30301 ttctgtgtca gaatatcttt ctaacacagt attcattcat aaaaggcctt cggttagtga
    30361 tcaggtatga tcgctgacca agtccttttt ttgttaaagg agggaggaaa aatttatgcg
    30421 ttctttagat gatccaatca ctatgctggc aggagtcgga gaaaaaagag cagctagtct
    30481 tgccagcttg ggcatccaaa cgattgaaga cttactgact tattatcctt ttcgctacga
    30541 agatattcag gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta ctttgaaagg
    30601 aatcgtagtc tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc gattgcaatt
    30661 tcgtatgatg caagaacatg atgtgttcaa cgtttctttt tttaatcaac cttatctaaa
    30721 agaaaaagtc attttatcgg aggaaatcgc cgtatacggt aaatgggatg caaagcgtaa
    30781 agccttgaac gggatgaaga ttttaggttc tcaaacaatg gatgactttt cgccgatata
    30841 tcacgtcaac aaatcaattc gtcaaacgac attagttgat ctaatcaaaa aagggtttca
    30901 agaatacggc acattgattg aagaaaatct gccaaacaac cttgtggaga aatatcgact
    30961 gcttgaccga gcttctgctg taagaagtat gcattttcca aaagaccacg aagaaaacca
    31021 tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc gtcttcaagg
    31081 attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag atcctttatg atgtccaaaa
    31141 attgaaacaa tttactcaaa agctaccttt tgaactgaca aatgcacaaa aacgtgtaac
    31201 gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt tgcagggaga
    31261 tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta tacgctaccg ttacagcagg
    31321 tttccaaggt gcgttgatgg tgcctacaga aatcttggca caacaacata tggaaagctt
    31381 gcaacagctg tttgatccca atgaagtaac catcgcattg attacggggt caacgaaagc
    31441 aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg tcgtgatcgg
    31501 cacacatgca ttgatccaag atggagtcga atttcagaat ttaggtctag ttattacgga
    31561 tgagcagcac cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag gctggcgacc
    31621 agatgtctta tttatgacag cgactccgat tcctagaaca ttagctatta cagcatatgg
    31681 tgagatggat gtatctatca ttgatgaact tccagctgga cgtatcccga tcgaaacacg
    31741 ctgggtaaga acacctcaat tggacagtgt actggattgg acgaaaaaag aactggcaag
    31801 aggacatcaa atgtatgtca tctgtccgct gattgaagaa tcagaagcat tagatgtaaa
    31861 aaatgcagtg gaaatctatg aaaaacttcg tgaactattt gcaccaaaat atgaagttgg
    31921 cttgttacat ggtaaaatga agaatcagga aaaagaagta atcatggaag ccttcaaaga
    31981 caaccagatg cagatattag tttcgactac tgtaattgaa gtaggggtaa acgtgccgaa
    32041 cgcgaccgtc atgctgatca tggatgcgga tcgatttgga ttggcacagc tgcaccaact
    32101 tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt atcttagttg ctaatcctaa
    32161 aaatgaacta ggtgtggaac ggatgaaaat catgacagag accaccaatg gttttgtact
    32221 aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta tttggcttcc gtcaatctgg
    32281 tctgcctcaa tttgcgattg ctgacttggt gacggatgga aatgttttgg aggtagccag
    32341 agaagaggct gcagcccttt ggcaaataaa ggattggcag atgcttcctg agtatcagcc
    32401 tttagcgcaa agcctccaaa tagaaaatca ggataatcgt tatttcgatt agcagactgt
    32461 gctatacttt atactgatga cggaaaaagg agtggaaaca atgaaaattg ccgtagatgc
    32521 aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc gtggctttag cgcagcaaga
    32581 ttttccagaa gtagaattct tactttacgg aaaagaagca gaaatcaaga aatacttaac
    32641 aaatgaaaaa aacatcacga ttatccatac agacgaaaaa atcaatagtg atgatgagcc
    32701 agtaaaagca attcgacgaa aaaagactgc atcaatggtc cttgctgcac aagcagtgaa
    32761 aaacggtgaa gcagatgctg tattttcagc agggaataca ggcgcgctac tagcagcagg
    32821 gttgttcatc gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt ctaccttgcc
    32881 agtagtaggg aaagaaggtg ccggtttcga tatgctggat cttggcgcta acgcagaaaa
    32941 caaaccagaa catttgttgc aatacggtat tttaggttct ttttatgcac gtaaagtccg
    33001 aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa caaaaggtag
    33061 tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa acaagcttga attttattgg
    33121 aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat gtagtggtca cagatggatt
    33181 tacaggcaac gctgttttga aatctattga aggaacagca atgaatatga tgaatctatt
    33241 aaaatctgct attctaaatg aagggatcaa aggaaaaatg ggcgctatgc ttttaaaaga
    33301 cggcttacgt tctttgaaag cagaaatgga ttattccaaa catggcggtg ccgttttgtt
    33361 tggcttgaaa gcaccagtca ttaaaactca tggagcaaca ggccccgatg ctgttcgcta
    33421 tacgatccgc caaatccaca caatgcttga gacagatgta gttggacaat tagtcgaaca
    33481 atttgaaaaa agcgaagaat agtatttgga tatctgaagc gatccgttcc attgaaaaaa
    33541 aggtggacat tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa ttgacaagtg
    33601 tatgattctt ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa gtagcgaaaa
    33661 ttatttcaaa tcattttgag atagataccg ataaagtgac ggatgaattg aacatcaaag
    33721 atgatttaaa agctgattca attagtatta tggaattcgt tttagagctt gaagatgaat
    33781 tcggtacgga aatttcagat gaagacgctg aacaaattga aacagtcggt ggcgcagtag
    33841 attatatcag cagccatctt aaataatttc aaataaaagc aaagaaggat aaaaagctgc
    33901 atgcagcttt ttatccttct ttttttaatc tttttgacac gtaaaagtca tttttc
//
LOCUS       DACDSY010000011        32167 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDSY010000011 DACDSY010000000
VERSION     DACDSY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.011"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(105..485)
                     /gene="yajC"
                     /locus_tag="IWK44_000496"
     CDS             complement(105..485)
                     /gene="yajC"
                     /locus_tag="IWK44_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP9549052.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(582..1727)
                     /gene="tgt"
                     /locus_tag="IWK44_000497"
     CDS             complement(582..1727)
                     /gene="tgt"
                     /locus_tag="IWK44_000497"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP9549053.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1945..2643
                     /locus_tag="IWK44_000498"
     CDS             1945..2643
                     /locus_tag="IWK44_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9549054.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2811..5450)
                     /locus_tag="IWK44_000499"
     CDS             complement(2811..5450)
                     /locus_tag="IWK44_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9549055.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6069..7100)
                     /gene="queA"
                     /locus_tag="IWK44_000500"
     CDS             complement(6069..7100)
                     /gene="queA"
                     /locus_tag="IWK44_000500"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP9549056.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7293..8468
                     /locus_tag="IWK44_000501"
     CDS             7293..8468
                     /locus_tag="IWK44_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9549057.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8472..9113
                     /locus_tag="IWK44_000502"
     CDS             8472..9113
                     /locus_tag="IWK44_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9549058.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9110..10027
                     /locus_tag="IWK44_000503"
     CDS             9110..10027
                     /locus_tag="IWK44_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9549059.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10024..10695
                     /locus_tag="IWK44_000504"
     CDS             10024..10695
                     /locus_tag="IWK44_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9549060.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11236..11724)
                     /locus_tag="IWK44_000505"
     CDS             complement(11236..11724)
                     /locus_tag="IWK44_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP9549061.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11925..12191)
                     /locus_tag="IWK44_000506"
     CDS             complement(11925..12191)
                     /locus_tag="IWK44_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9549062.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12191..12454)
                     /locus_tag="IWK44_000507"
     CDS             complement(12191..12454)
                     /locus_tag="IWK44_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9549063.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(12447..12719)
                     /locus_tag="IWK44_000508"
     CDS             complement(12447..12719)
                     /locus_tag="IWK44_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549064.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            12930..13115
                     /locus_tag="IWK44_000509"
     CDS             12930..13115
                     /locus_tag="IWK44_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9549065.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13257..15080)
                     /locus_tag="IWK44_000510"
     CDS             complement(13257..15080)
                     /locus_tag="IWK44_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP9549066.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15678..18383)
                     /locus_tag="IWK44_000511"
     CDS             complement(15678..18383)
                     /locus_tag="IWK44_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP9549067.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18689..19636)
                     /locus_tag="IWK44_000512"
     CDS             complement(18689..19636)
                     /locus_tag="IWK44_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP9549068.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19826..20095
                     /gene="rpsN"
                     /locus_tag="IWK44_000513"
     CDS             19826..20095
                     /gene="rpsN"
                     /locus_tag="IWK44_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9549069.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20271..20633)
                     /locus_tag="IWK44_000514"
     CDS             complement(20271..20633)
                     /locus_tag="IWK44_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9549070.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20644..21765)
                     /locus_tag="IWK44_000515"
     CDS             complement(20644..21765)
                     /locus_tag="IWK44_000515"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP9549071.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21781..22131)
                     /locus_tag="IWK44_000516"
     CDS             complement(21781..22131)
                     /locus_tag="IWK44_000516"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP9549072.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22261..23772)
                     /locus_tag="IWK44_000517"
     CDS             complement(22261..23772)
                     /locus_tag="IWK44_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9549073.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24094..25446)
                     /locus_tag="IWK44_000518"
     CDS             complement(24094..25446)
                     /locus_tag="IWK44_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP9549074.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25599..26675)
                     /locus_tag="IWK44_000519"
     CDS             complement(25599..26675)
                     /locus_tag="IWK44_000519"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP9549075.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26871..28616
                     /locus_tag="IWK44_000520"
     CDS             26871..28616
                     /locus_tag="IWK44_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP9549076.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28896..29333)
                     /locus_tag="IWK44_000521"
     CDS             complement(28896..29333)
                     /locus_tag="IWK44_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP9549077.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29317..30690)
                     /locus_tag="IWK44_000522"
     CDS             complement(29317..30690)
                     /locus_tag="IWK44_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549078.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31012..31500)
                     /locus_tag="IWK44_000523"
     CDS             complement(31012..31500)
                     /locus_tag="IWK44_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9549079.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31522..32124)
                     /locus_tag="IWK44_000524"
     CDS             complement(31522..32124)
                     /locus_tag="IWK44_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9549080.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9195 a         6877 c         5940 g        10155 t
ORIGIN      
        1 aatattgagc aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta
       61 ggacatcttt ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc
      121 tcttctgtct cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt
      181 tcttctgttg tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc
      241 gttttgattg ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc
      301 cgtttgtcat tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa
      361 tcacctggtt tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa
      421 cgtgacataa aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt
      481 cccatttttt tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct
      541 gataacacta gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt
      601 tctttattga acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc
      661 atgattgctt gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa
      721 gtcaaacgaa tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta
      781 tagtttttac atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat
      841 ttcgcatttt tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt
      901 cgagtaggca agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa
      961 tcagcagcac cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg
     1021 aattctaata cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag
     1081 taaccaggga aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac
     1141 cctgctcctt ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat
     1201 cctcgttctg cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca
     1261 tcgaatggtg gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg
     1321 ttgattgctt tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc
     1381 acgccttctt cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa
     1441 tctgttaaaa tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact
     1501 aagtcttcac cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc
     1561 atttctttca actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg
     1621 aacattggtg tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta
     1681 tgtttttctt tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact
     1741 ttctatataa aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa
     1801 gttccctcgg cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat
     1861 aacaaaaaaa agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt
     1921 attaaagaaa aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa
     1981 gagagtctaa aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt
     2041 ggtattgcta ctattcttct tattatgatt cctattatct tgtttgccac caccatatat
     2101 gatggatcag cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt
     2161 ggcggaatta tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat
     2221 cttgatattt tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga
     2281 ttcaaaggtt tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca
     2341 acattatggg gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa
     2401 agctatttta tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat
     2461 acgatcactg caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat
     2521 ctttcattta tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg
     2581 ctcagcccat acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt
     2641 taaaagagag tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc
     2701 agtaaaagaa acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc
     2761 tcctttttgt atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct
     2821 tcttctgctt ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata
     2881 gataaaatcc ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct
     2941 tgcgtaaatg gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct
     3001 accaaagctt tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg
     3061 aacacatgca gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc
     3121 tgacttgcgt ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac
     3181 aaggttacga tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca
     3241 aagatccctt catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct
     3301 cttcctaaag caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca
     3361 gctgtaaatg gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa
     3421 atattacagc ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct
     3481 tgactgacag cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca
     3541 ctttgcgcca catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac
     3601 gctggtgcat cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa
     3661 gatttgacga tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg
     3721 acacgcgcat ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct
     3781 gacggatcat tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct
     3841 ccagtgatca tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt
     3901 tctggacgtg gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt
     3961 gcttcagaag aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact
     4021 ctcagtgccc gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa
     4081 ctaaagcgcg gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc
     4141 atttggtgaa ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt
     4201 ggatattttt cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg
     4261 gctacttctg ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca
     4321 tctgtacata gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg
     4381 atattcgtta cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc
     4441 agcgttcctg ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc
     4501 aactgtcgga tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac
     4561 gtaacaataa cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg
     4621 aacatctcaa ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc
     4681 gctaaagcga tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt
     4741 ttttgcatag tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca
     4801 atggcagtga cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata
     4861 ttaagttggt cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact
     4921 gcttcacttt ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa
     4981 cgggcatcgg cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt
     5041 tgctctgact caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc
     5101 atatcttgca aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc
     5161 gagttgatca caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta
     5221 gcaaatgcgg tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg
     5281 gacaagcttc gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc
     5341 aatccctgct tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct
     5401 gcttctgtta ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc
     5461 ctttaaaatc ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta
     5521 cttcaaataa agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata
     5581 tttgcaaacg ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa
     5641 tagacagaag aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt
     5701 ttcacccaga catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg
     5761 gatcacgaag tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg
     5821 tctgaaaatt gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa
     5881 tttttgttta acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa
     5941 tcatttctgt ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg
     6001 gctttttctt tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt
     6061 tcataacctt atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca
     6121 atcgcatgct ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg
     6181 actaaagtcg attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca
     6241 tagccaggcg taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat
     6301 ttcgttccaa tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg
     6361 ccatttttac gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca
     6421 ctatgcatat ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct
     6481 acatgtagtg tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct
     6541 ttcgtaaaat gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca
     6601 gtttgatagc gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct
     6661 cccaatgatt caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg
     6721 ccgtgttcca attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt
     6781 gtcccaactt tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta
     6841 ttcgttagaa gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga
     6901 gcaggcagaa ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg
     6961 atttcatgga aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga
     7021 gaatgatcgc ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca
     7081 aaatcttctg ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc
     7141 atgtttttcg ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa
     7201 ataaaaaaac taccctattt gtattttttc attgttttag gcatactagt aatagatagt
     7261 tcttcattga aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa
     7321 tttataaagg tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt
     7381 tcatttgttt tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc
     7441 gaatgactga tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg
     7501 atccagtgga attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc
     7561 acatgacgat ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag
     7621 aacggaataa aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag
     7681 atcgctaccc acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc
     7741 ttgcagcgaa tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca
     7801 ctcgtgattc cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg
     7861 tatttgtaac ccatgacatg gatgaagcga ttaaactagc aagccgtatc gccatcatga
     7921 gtgaaggccg tgtgatccaa tttgatacac cagaaaatat tttacgtcat cctgcaaacg
     7981 atttcgtaga agagttgatt ggagaagatc gcctactaca agccaagcca aatgcgacaa
     8041 gagtcggcga agttatgcta aatacagcaa tcacgattac accggaaaaa tcattacaag
     8101 aagcaatcaa actgatgagg gaaaaacgtg tagatacgtt gttagttacc gataattcga
     8161 atgtattaaa aggatttatc gatgtagaaa cgttgaataa aaaacgagga aaagtatcca
     8221 gtgtcggcga cattttgaat aaacatgttt tttacgtaaa agaatccgcc tatcttcgtg
     8281 ataccttgca acgcatttta aaacgcgggc tgaaatacgt gccggtcgtt gatgaacaga
     8341 acaaagtcgt aggtatcctg actcgtgcat cattagtgga tatcgtctac gatgtgattt
     8401 ggggagaaga tcccgcaact actgatgttg cagagtcagc tactcctgaa gaatctaagg
     8461 aggtctgacc tatgcagcag ttttttcatc agcacggttc agaaattctc tcaaaaggtt
     8521 ttgaacatct ttatatttca ggacttgctt tgatcattgg tatcatcatt gctgtaccgc
     8581 ttggtgtctt actgacacgt tttccaaaag ctgccacgat tgtgattggc ttaaccagtg
     8641 ccttgcagac aatcccttct cttgctttat tggcattgat gatcccgcta tttggcgtag
     8701 gtaaattacc tgcgattatc gcactcttta tttattcatt attgccgatt ttacgtaata
     8761 cgtacattgg gatgaaaaat gtcgattgga attatcggga tgtggcaaaa ggcatgggga
     8821 tgactgagtt ccaatccatt ttttccgttg aattgcccat tgctatgcca acgatcatgg
     8881 ctggtattcg tttagctgcc gtttacgtga ttgcatgggc tacacttgcc tcttatatcg
     8941 gcgctggtgg tttaggcgat ttgattttta gtggactgaa taattaccaa ccagatctca
     9001 ttttggcagg tacgatccct gtaacaattt tagcactatt ggctgattgg cttctaggtc
     9061 tcttggaaca ccggctgaca ccgatcgcac ttagagagga gaatgaagaa tgaaacgtat
     9121 actaaaaatg accatggttc ttttctctct tcttatactt gcgagttgtt ccttccctgg
     9181 tcttgccagc aatacagatg aagacaccat ttcgatcaca ggtggaatca cttcagaagc
     9241 acagatctta gcaagtctag ttgccggaat ggttgaacat tatacagata aaaataccgc
     9301 aatcatcaac aatctggcta ctacaactat caaccatcaa gcgatgatga acggagatgc
     9361 ttcaatttca gcggctcgct acacaggaac agatttaacg actacattga accttcctcc
     9421 tgaaaaagat ccgaaaaaag cgtttgctac cgtgaaagat gaatttgaga aaagatacgg
     9481 tcaaacgtgg ttcccgtcct atagttttga aaatacgtat gtctttcttg ttcgaaaaga
     9541 caccgcacaa aaataccatc tatctaaagt cagcgatctg aaaaatgtag cggatgaatt
     9601 agtagctggt gtagatactt cttggatcaa tcgtaaagga gacggttatg acggcttcca
     9661 agaaacttat ggcttctcct tcaattcgat attacctatg cagattggac ttgtttatga
     9721 cgcagtcgaa gcaggaaaaa tggatattgt tcttggctac tctacggatg gacggatcgc
     9781 cagttatgat ctagtcatgt tggaagatga tcgtcaattc ttcccaccat atgatgctgc
     9841 tccagttgta gacaacaaga tattagctga tacgcctcat ttaaaagaag ccttaaataa
     9901 gttaggtcat accatcagta cggaaaaaat gcaggaattg aactacgaag cagataataa
     9961 tctagtggaa ccatcagtag tagcacaacg tttcttacaa gaaaaccact attttgaagg
    10021 gaagtgaagc aattggataa aatgaatgtc tttcaacaat ttttctatta tttccaggaa
    10081 aatggctcct atatctttgc acaatttatc cgtcacttcc tgatttctat ctatggtgtc
    10141 ctctttgcag cagttgtcgg gattccggtt gggatcatga tttctaggag acgtaaatta
    10201 gcaaactggg tgattcgttt ggcgaatatc atccagacga tcccttctct agccatgatc
    10261 tccattttaa tcattggatt aggcctggga gtcaatgtcg tgattgttac tgtatttttg
    10321 tattccctct taccgatcat caagaatacg tataccggga tgatccaagt agacaaaaac
    10381 atccttgatg tcggcaaagg catggggatg actgctagac aacgactatt catggtagag
    10441 ttaccactct ctgtttctgt tatcatggca ggcataagaa acgcgttagt tgttgccatc
    10501 gggataaccg cgatcggtgc attcgtcggt gctggtggtc ttggagatat cattatccga
    10561 ggaaccaatg caacagaggg cacatcgatt attttagcag gtgcattgcc aactgcatta
    10621 atggcgatca tcacggactg gcttttaggt attttggaaa gacggctaga tccagccagc
    10681 cgtacatcac gctgatgtta ttgtaaaaaa caaaagcttc tgcgaccatt tgttggtcgc
    10741 aaaggctttt ttagttctgt catttttaag gcaataatcc actctgattc ttccctcctt
    10801 atgggtaaag agttgctttt cttagctatt tatccattcc gtatactcca acttcatact
    10861 gccaacgggt tcatatctgc atccaccgac taagtttttt tcttatccgc taaggcattt
    10921 tgccaatcca ttttcccatc actcgtcatg gaattcatac cacatgatct ctaaatactc
    10981 gtctgcttgt tctttgaatt gggcatcaat tcttacatcc tttggcgtgg ctttcattta
    11041 agccttttcc atcagtcctc gattgttcga tgccttcagc tgcatcatca aaactggtga
    11101 ctgccgtatc ggtaacttcg atcatctttt atacaatcgg gaacttgcca tcctcatcct
    11161 tggctaaaaa catatctagc cattccagct tctcgccatg ggaggtttgc ggttcctaaa
    11221 accggtacac gattcttatg gactgcatcc gtcgcaacta gacttggcga tacgatgatc
    11281 cttcaccaga agagcacccc cgtaaaccaa ttgatcgata tattgccaat aagaaaagac
    11341 attggcgtaa aagatatcga tccctcgtga tgcattgccg ttgatactgc tattcatggc
    11401 cgaaagcgcc acgactttaa cttcttccgt ttgagtgaca ttcatgttcg gcaatgtcgc
    11461 tttggatacc cgctctgcta aaggaacgac actggtattg tatttcgtat ctgtatcttt
    11521 tccaaaagac catgccaacg aatcctctgg gaaccaataa gacgactccg gttgattgtc
    11581 cattgctttt ttcaacatac ttttggctgt ttctcgataa acgacatttc caattgtctt
    11641 cgcctcactt tggcgataat cgatggtcgt tgccactgcc aacactaaca acgatgctcc
    11701 aattttactg attttccttg tcacacttct tcctccttgt tttacataaa cccgtcaaca
    11761 ttccattcct aaactcaaac aatgaccctt caaaggtgga gatataggaa caaattaaag
    11821 aaatcatgca ataaatccaa gaaggttggt tttagaacaa aaaaagagtc tcctttttgg
    11881 ggagactctt tgttaactat ctaaaaaggt tggctcttct cacttcactc ataatgtgtc
    11941 caggcagaat aaatttctac aactttattt tcctcatcaa ctttgtaaac cactcgatgc
    12001 tgtctattca gcctgcgtga gtatctgcct tcatgtgtag gtctcagctt ttcaaaagac
    12061 tgcgtgggca tatatggatc ttccttcaat gtctgaatca cttcttcaaa ttgtttcttt
    12121 aaattcgttt gcttgatttt tcttaaatcc actttcgcag agtttttgat tgcgactgta
    12181 tagttactca tcaaagagct tcccaatcaa tatcatcgat attcgtaaag ctactgtcgt
    12241 ctttttcgcg gtctcgtact ttatctaaag tacctgtttg ctcaagaaaa agggtttctt
    12301 gaatcgctct ccagtcttcc agcccaatca atacagcatt gtttccacta cgatcactaa
    12361 taatctcgat ttcagtatga ttttcattta cctctttcaa taactgataa aaatttttac
    12421 gagcagtgct aggattcatc gttgtattag ccattgtctc tcctcctttt tctgatataa
    12481 ttatagcgta cttgattaag tacgtcaaaa aaatacaata aagcacttaa attcagcaaa
    12541 cttttcctct tttttacata gagaaaaaga ccccttttta gggaggatct ttgtccacgc
    12601 tcacttctta ctcctttacg actagcggta gccaaacgta aagtaattgg taacaacgta
    12661 tcatctttag caaaggtcga atccaaagcc tgtgagtcta tgcaacaaca ccaatacacg
    12721 aaggttcctc gttgcagtgt gacttgattg atatcttgaa taacctgagg atacgcacga
    12781 aagatttgta acggttattc aaaactgatt ttggccgtca agtcttctac tgcctcaaaa
    12841 atgattgcct ctttcgtctc ctgtcattca tgttcactat cgatttgata cttgcttaga
    12901 ccgttgggtt tgtgaatcac caattaaaaa tggcactgag ttatggtaac aaaaaatggt
    12961 cacttgacga ggctgagatt cctacttatg aagacacatg gcacttaaga gtcgaggtaa
    13021 aggaagaagc tgaaaatata ggaggctgta cattatttgg caaaggattc ggaaattatg
    13081 atcaagacat caaaatcaaa atgttttaca gctaaatagg aagaataata acatttcata
    13141 cgctgttccc atctttaatg gcttatagag ggcaccttta gctgagaata agacaaaaca
    13201 gaccctctag cttcaattaa gagctagagg gtctgttttt gattttaaaa cttggtttat
    13261 ttcttcaaat ggtagctata ggtgctgaca acgatatttt cacgccaaga caatcgcaat
    13321 cttaaccgca aacttgtttg gttatggaga atattctgcc agcgtctgtt tggtacaaat
    13381 tgaggaatca acacagtagt tgtatagttt tgtttcgctg catttttact tacgatatct
    13441 acataacgga taattggatt ttcaatcgag cgataagaag agtggacgat tgaaaaacgg
    13501 acatctggga aatgcttttt aaattctgct tcgatttctt tttctttttc gacatcttcg
    13561 tctagcgaaa catgcatcgc aacgacataa tcaccgatcg aacgtgcgta attcaacgca
    13621 ccgatattta cccgtgtcac attcccgact aatacaagaa cggtattccc gtcataatca
    13681 tgtagttttg catcattttc taaacgaagc tgttctgcta cttttttata atgatcatgg
    13741 atcttataaa aaatgaacat aacgattggc ataatgatga agaaaggcca aatgtcccct
    13801 aaccgataga caaacaaaat cgcaatgatt gcatatgaga tgaacgcacc cgtaatattg
    13861 gcaatcgatt tgcttaacca tctttttcct tcttttttcc atttgacgac catccctgtt
    13921 tgagataaag caaatggaat gaacacgccg atagagtata acggaatcaa gcgttctgtt
    13981 gagccatgga aaataaacag caatacgatc gaacctagcg ctagtgtgat aatcccattg
    14041 gaatagccta gtcgatcccc acgatcttga tacatgtgag gcatgaattt atcttttgcc
    14101 agattgtatg caagaactgg aaaagcagaa aaaccggtgt tagcagcaac tgctaaaatc
    14161 agcgctgtag caaattgaag gacgtaatac aaaataccat gtccaaagac agctttacca
    14221 atttgagaca aaacagtgac ttctttttca ggaacgatcc cataccaata gttgataaat
    14281 gtgatcccca cgaagaagaa acctaagatc aatgccatca tcgtcaatgt agcagcggca
    14341 ttttttgctc ttggcttctt aaagaatggt acagcattac taatcgcctc caccccagtt
    14401 aaagaggaag aaccagaaga aaatgctcgt aaaatcaaag cgatagaaac acccggcaca
    14461 gccgctcctg gaagtgcagt agcttgcaat ggcacagcac ctgtcacgat attatataaa
    14521 ccaacaacga tcagtaacga gatcacaagg ataaatgtgt acaccggaaa taataggaac
    14581 gatgcagatt ccctcagccc acgaagattc agcatcatca gtagcaaaac gattgtcaca
    14641 gaaatagcga cttgatgacc gtagagtgct ggcaccgctg atgtgatcgc ttcagcacct
    14701 gctgagacag atactgctac tgtcaacatg tagtcgatca ataaagagcc accggaaatc
    14761 aagccagcat tctttccaag attctcactg ctgacgacat atgctccccc gccatgagga
    14821 taagcatgga tgatttgacg atatgatagc gttaatgaaa tcaataaaat aataacaaaa
    14881 gcggcaatgg gtagcgaata ccagatggct gcagcagaca aagcgactaa aacgacaacg
    14941 atttgttccg taccatatgc aatagatgat aacgcatcag acgacaataa tgccaaagca
    15001 gcaaatcggg ttaatttgtg ctcatcattt tcagcagatt ttaaaggctt acctactaac
    15061 aaccttttta agtaatccaa tttctaaact cctttacaca caattttact tcaagtttta
    15121 tagtcgaaaa tttatagtaa acaagatgtt atagtacgct cactgacctt aaaagtcaat
    15181 aaactttaag aaaataattg gaaaaaaaca gaaaaatatc aagcgctttt tcaaacgctt
    15241 taatacagca aaaatctaac atttttttat gggctttttc tttgaattct ataataaaag
    15301 gatagaactt gacttagaaa atcaagttct atcctttttg atatcttaac ttttggaaga
    15361 gagatgtaag gctgctttct acccatcatt cttactactc gaagcatagc gcagttgtca
    15421 caacctcttg ataaacggtg tttaaaagct tgcatctgcg gtaataagcc caaccaaatt
    15481 aaaaacataa gaagctgttt ttatttggtc gttcttatta cttgatgcag agcagctttt
    15541 ttacaacctc ttggtacacg gtgttcaatc agctgaccct cggtattagc tcaaaaaaac
    15601 ggcaaaatat gaggaactat ttttccattt ttttctctaa tactcaggtc aaaacactga
    15661 tttcacaacc tcttttttta atgaaaaata tggctctcat gcgccgcttc tttgaatttc
    15721 gtcatttttt cttcgatgaa cggatagatt gccgttccta cgattccaaa gacaacaaac
    15781 aaaccgatca tgatccagat tgctttcgta gcatttggcc aataaattcc tcctgcagat
    15841 tcccgtaaca agtttaccgc atgagtaaat ggcaagaacg gattgatcat ttggaagaat
    15901 ttccctgaga cttggatcgg atagttcccg cctccgccag agatcgacag tactaagatg
    15961 ataatagcga tccctttacc gacattatcg aataatgcag ccaagacata gacgatcatc
    16021 atgaacgcta aggcgatcaa gacagcaaat gacacgctgt agaccggatt ttggacatct
    16081 actcctagta agaaaatatt ccctaatgta acgatcaagc tttgcgcaat cgccattgtc
    16141 aaaaatgtca gcatacgagc aacgaattgt tctcgtttcg taaaattgtt tcggtctttc
    16201 tttcctaaat agtattctgt cgtagcaaca cttgaaagaa gaacagcgcc gacccacaga
    16261 catagtgcag tataaaacgg ggtgctagct gaaccgttat ttgcgattgg ataaagcgca
    16321 ttggtttgta gctcaacagg ttgtgtaaag aagtcacttt cttttgttgc gtctagtttc
    16381 aataatttga tgacttcgcc tagatcgact tcttgttcgc cttttcggat cgcgtctgct
    16441 gctttttgga taccagcttt gatatttggc cagtcattgt tgatcaagtc cgcagcagcg
    16501 ttcacagctg tttcgacttc tggcattttt tcatttactg ttgtcaatgt accggtgatt
    16561 tcatttttta gtgctggcca atcattttgg ataaaatcag cggccaatcc taatttttgc
    16621 tgaatgatcg gtaattcatt gttgtatagg tctgcccctt cattgatgcc attgacgatg
    16681 gtatccatat ttccattcag cattgtattg gcatcatgga tttcttgtcc aattgcaggc
    16741 atttcttttt gatatttttc taaaattgcc actgcattcg ttacagttgc tttcgttgag
    16801 ctgagaagcg aatcaaagtc gatctggtcc gctttgctca gtgcgccttg tgcagtccca
    16861 atcgtatcga tgacttggtc tagtagagaa gtgacagtgg aactgatttg acctgtattg
    16921 ataccagcaa tgatgccatt gatattatta acaacggcat caatttgatc tagcaattca
    16981 tcaatagcct tcgtgtcttc attttgaaca gctgtgttca aagcagatag gcggttttgc
    17041 agattgctta gcaagggttt taattgattt agctgatcaa taattggctg taatttcgta
    17101 tttccagcac ttgtttgcaa tttagtcagg aactcgacca gtttatcgat tgcattttgt
    17161 tgttgagata ttccgtcaat aaagccgttc aatacattgc tgatgttttt cttttcatca
    17221 tcagtcagct taccatcttc aatagctgtt cttacagtat ctattaacga acctgtcgtt
    17281 gttgccactt gtccaattga cttcaatgta acatctacac tgtttgtgat gcttggtatc
    17341 gcatctttca gctgagaggc acctgatttt gtttggtttg ctagatcact tgcctgattt
    17401 tctaaatttt tcacatctgg caagatatct tgtacttgtt ggatgatcgt caagccttgt
    17461 tttgcttcat cgattccttg gttcattgtc tcttggatcg aagcaaaatc gccgtcgatc
    17521 tctgctagtt gcttaccggc attttggatc tctgggattt tttcttgcaa atcaaggatg
    17581 atttttgctt gatctttcaa ctcaggcatt ttgtcattca atgcaacgac tttttcaccc
    17641 atctcatcta ctttagggat gtaatccacg aattcatttg ctttattcag tttttccttg
    17701 atttcaggta gttgactttg taattcgagg acttgttttg tgtagccgtc gatcgtgtcc
    17761 aaattttcat ctgttgataa aatcatatct ttaactttat tgatacttac taggttcgta
    17821 tcgatgtcgt aacctatctc attgaatact ttcaataaag tcgaactggc tgttttgata
    17881 aattcatttg tgatttgcga ttggatcgaa cttgctcctt tatcggtgat tttcggtgca
    17941 atcgcattga ttttttcatt taccgtatat tcgatttttg gtttcttgat atcaccacta
    18001 gtaaaactca gcagatcttc tgagaaatcc tttggcaagt aaatacctgc gtaatacttt
    18061 ccggaacgga ctccgtcttc taattgttct tttgaatcaa cgaattgcca tcctaattgt
    18121 ttatttttat gaagcgattc gatgacttgc tccccaatgg ccacttcttt accttgaaac
    18181 tctgctggct tgtctgcact gtaaacagca attggcagct ctccagtatt tccatatgga
    18241 tcccataatg ctttgatgtt aaaccacgca tatagcgaag ggatgatcat gatcgcgatg
    18301 atcagaagtg ttgcaatcgg gtttttgaaa atccgtttcc agtcaagcaa atataatcga
    18361 agcgtatttt tgattgactg catagttctc ccccttcttc ttttttcagc tgaattactt
    18421 taccatgtat ttcatacttt tttcaatcgg cttttttcaa aaaagtcgaa tttttggaca
    18481 tatcacggca attgtctcat tgtaatctct tacatattga gaaataatta aggattacag
    18541 cctttacttt atcatgaaat aaaagggaaa gataatttta tgccaaagtt tttatctatt
    18601 atacgtatac gcaaaaaagc taaaacattt tttgtctttg cccctacaaa aatgtttcag
    18661 cttttttcgt tttttattcc tgtatttctt atttttcgaa tgacaggatg attttcccag
    18721 caggaggatg tccttcaact agctgatgtg cttggatgac atgcttaagt gaagcagaca
    18781 gattcattga aataaaaaca tgaaaggctc ctttttggta ggctttgatt atgccagcaa
    18841 aggcttctgc atctagatat tcctttcttg gaacaaacga ttcataaaag ccttcctttt
    18901 tttgccgcaa atctagatct gggaggtcat ttaatgcaac atacctgccg cctggtttca
    18961 tgatctggat gccggtttct cctttgatac ttcctttcgt cgcatcgatt acaaggtctg
    19021 cctgatcagc aaattgcaga cctggatttg ttttatcata agcagcaaat gctgaagctc
    19081 ctaatttttt taccttttcc tcatttttac tggatgcact ggttaagatt cggattccct
    19141 tttcatggag aagctggatc agacttgacc cgaccgcacc tgatgctcct agtatcatta
    19201 cagtatctgt cggctggatt tccgtcaaat ggttgattag attataagcc gtaattcctg
    19261 gcgtgaccat tccggctgct tcttcccaac tcattttatc tggaattttt gctacttttg
    19321 ctgatggcaa cacgacttct tctccgtaag tcccgccgac tgcatggaca gctactcgat
    19381 cacctacatg aacagttgtg acatctgaac caatctctgt cacaatacca gcgccatcat
    19441 tacctggtac ataagggaat ttcaatgttc gtatctcttt catttctcct aaacgcaagg
    19501 caacatcata aggattcaca ctgaatgctt ttatttctac acgaacatgt ccagaaccga
    19561 cttctctggg atgagcatcg atttcttcaa aaacatcttg tgcttctccg taaccgttaa
    19621 ttgcaaatct tcgcatggtc accatctccc tttttcttta agtttacgtt tatgccttta
    19681 ttttgacaaa aaaaatgtct tttcgttttg gcgcatgatt caaaacaaaa tagttgtctt
    19741 ttttcgcgta acgtgttaaa ttatttatct aaaagcgtaa agattacgat ttacgctaat
    19801 aaaaaataat tggaggaatt tgttgatggc taaaaaatca aagattgcca aagcaaaaaa
    19861 acagcaagaa acgattgaac gctatgctgc tttacgccag gaattaaaag cgcaaaaaga
    19921 ttacgaaggc ttgcgtaaac ttcctttaga tgctcgtcca gaacgattga aaaatcgtga
    19981 tttaatcgat ggacgccctc gcggatacat gcgtaaattc gggatgtccc gtataaaatt
    20041 ccgtgaatta gcacataaag gtttgattcc aggagtaaaa aaagccagct ggtaatccaa
    20101 gtctggctgt aatgtaaagg aggagggcgc atggaccact ccttggatca tcaaaaaaga
    20161 tacgttcgct tcttctagca gaaatgctgg tactagaagc agacgtatct ttttttacgc
    20221 acagctggtc cttttttata tgtaaaggct gcatggttac ttcgctatat ttatttagta
    20281 acaggagtca gtgcttcgat acccacacta attttcaatg ctcggtcaac ttctgccata
    20341 atgggtcgat ccaaatggca gaccttttct ttcaatcttg atttatcgat cgttctgatc
    20401 tgctccaaca ggataacgga gtctctttct attcctgttc cagtagcttt gatcccaatg
    20461 tgagtcggaa gcttaggttt agccattttt gccgttatgg ccgcaacaat gatcgtcggg
    20521 ctaaaatggt tacctagatt attttgaata accaaaactg ggcgtgttcc gccttgctcc
    20581 gatcccacga ctggagaaag atcagcaaaa taaatatctc ctctttttac cataggatcc
    20641 tccttattct tgatattctc ttggtatccg ctgtcctaat ccgcaagcca cttcatagtg
    20701 gatcgtacct aattgatctg ctatatcctg catagtgatt tctttgtctg catttttgcc
    20761 gataagcgtc acttttgttc cgacaggaaa ttcttgaggc aatcggatca tcagttgatc
    20821 catacataca cgcccaatcg tttcacagta gttcccttct accaatagag aaaaaccctg
    20881 catctttcgt ggccagccat ctgcataacc gatcggtatc gttccgatcc actctgcttc
    20941 aggagtgata tatgtttctc catagccgat tccttcacct gctgggagtt ttttgacttg
    21001 aatcaattca gaaaccagct caagtgctgg ctgcaatgga taaacttccg gtagtgcgtg
    21061 cccagaggga ttaagtccgt acattgctac tccataacga atcatattgc cgattgtttc
    21121 atcatgccat aaagccgttg cactattgct gacatgtaca tatcgaggaa gttcgggaag
    21181 ttttttcagc acttctataa agcgttcttt ttgcagattc caatatgtat catctgcctg
    21241 atctgctgta gaaaaatggg taaataaacc ttcccattct aatacacgac tttctttgat
    21301 gaatgcagct gcctctttta cttcttcagg tgtacaaaat ccgatacgac ccatcccagt
    21361 gtctgctttt agatgaatcg aaagaggcgt ctctagctta cttttctcta aaacagcagc
    21421 tgctgcttgc aaccattccc gtgtacctgc agttactgat aaatcatgag caacagctac
    21481 agtcagtgcg tcgtaaggaa ccatattcaa gataaggatg ggctgagtaa atccagcttc
    21541 tctcaactcg actccttcat ctaaatttga aacacaaaag cccgaagctc cacctgcaac
    21601 tgctgcctca gctgtttcaa tagctccatg accgtatccg ttcgctttta ctactgcaaa
    21661 caattctttt ccttgcggaa gtcttgcaac ttcatttgct acattttcag ttatagcttt
    21721 tttatgtatc actgctttcg ttgggcggtg ccaagcgaca accatcgttt ttccttcttt
    21781 ctagtcttcc aaaataattt gtgcaaaagc tgtttcattt gtatgagtaa tactgaccca
    21841 tacattgcct tcatgaggtg aacgcgtcac aacaggctga ccattttcat tttttaaaat
    21901 ctcgacatct tgaaaggtaa cttttccgat cccagttccc caagctttag agaaagcctc
    21961 tttgcaagcg taacgcccgc ctaaaaattc tacttggcga tgaaaaggca aggactggaa
    22021 tagtttcatt tcatcagagg tcaagattcg tgcaatgaat tttggttttt cttcgatcag
    22081 ccgagtgatc ctaggaagtt cgactgcatc gatcccaatt ccttttatca tatgcttttc
    22141 tcttttcaat aaatttagaa cactctcatt ctaccaaatt ataaccattt tgccacaaat
    22201 aagtccacaa ataacaaaaa tgaaagtttc tcttaaaaaa gagaaacttt cattttatgt
    22261 ttaatcgtta tttgaacgga ttacaaatcc gcgtttttta tcattgtgac gtttaccgcc
    22321 atctttttgg ctgttattat ttttgctata accgcctttt gatttgtttc cacgataatt
    22381 accgccgtca cgacgattac ggttgtttcc gccaccacgt ttgccatttt tattgtatcc
    22441 ttttttctgc attggcaatg ggcgttctgg tgtgatcttc actggcactg catcagctgg
    22501 atcttttgct gtcgttttta gcaaaagtgc aactaaatct tgtgcagaat attcttctaa
    22561 taatttatct gctgtttgca ggtatttatc tagaccattt tctgcaagtt ttgtttcgat
    22621 ttgttcaact gcagcaccta attgaccttt gaatgcttct ttttcggttg gtggacgtaa
    22681 agtcgtcatc cgttttttcg tcaagttttc aatcacatgc aagtagtcca tttcatttgg
    22741 tgtaacgaaa gtcacagaca taccgccttt tccggcacgg cctgtacgtc cgatacggtg
    22801 aacgtagctt tctggatctt gagggatatc atagttgtac acatgagtga cacctgaaat
    22861 atctaatcca cgtgcagcaa cgtctgtcgc tacaaggata tctaaatgac cgctcttaaa
    22921 tgaacgcaat acgctcattc gtttttgttg agacaaatcg ccatggattc cttctgcacg
    22981 gtaaccgcgt gcttctaatc cgcgtgccaa ttcgtctaca cggcgttttg tacgtccgaa
    23041 gacgattgtt aattcaggtg tttgaacatc aaacaagcga gtcatgatat cgaatttttc
    23101 gtattctttt gcgcggacat agtattgatc gatcaagtca gctgtcattt ctttcgcttt
    23161 gatcttcaca tgatgtggat ttttcatgaa tttaacgcca atattcttga tagctggcgg
    23221 catcgttgct gagaaaagta atgtttgtct ttgatcagga acttgtgaaa tgattttttc
    23281 gatatcttcc aagaagccca tattcaacat ttcatctgct tcatccaaaa ctaatgtttg
    23341 aacggttcca agtttcaatg tgtggcggtt aatgtggtca agcatacgtc ctggtgtacc
    23401 cacaacgata tgtggacgat ctttcaagcc acggatctga cggccaatat ctgctccgcc
    23461 gtaaacggct tgtacacgta cttttttgtc gcgccctaaa cgatataatt cttcttgtgt
    23521 ttggatagct aattcacgtg ttggtgcgat aactaaacct tgtaattcgt gacgatctgg
    23581 atcgattttt tctaacattg gtaatccaaa tgcagctgtt ttccctgtac ctgtctgtgc
    23641 ttgtccaata acgtcttttc cagctaatgc tagtggaatc gtttctgctt ggataggcgt
    23701 tgcttcttca aaaccagcgc gttcgactga tttcaacaat tctggtgata attctagttc
    23761 tttaaatttc aaatgaatcc tcctatatgg ttgttaactg aggatgtagc ttttttgcta
    23821 ccccgtcgat ttgttcggtc aaagtgtatt gtttcttttt gacagtggcc acgcacgttt
    23881 ttatccattc ttagcccctg tggcaccacc gaaaaatcac tttgcgaagt ttaggcatta
    23941 gaaaagccaa acgggtcgcg ttcagcttta tcattattat gacatctggc ttcaacaagt
    24001 tgtttctatg gcaaaaccag tccaatttct cttcatcttt cggcaatagg ttattctaac
    24061 atagtttttc gttttctgca agtttacaga tttttatttc atttgctgaa gttgttctac
    24121 cacttcaaat aagcccatgc tgttgctgcc ttttagcata atactatctg ttgcttcaag
    24181 attttgtttg atagcatcaa tcaatttatt cttttctttt tcaaaataga acacaggtag
    24241 ttgggggtat ttttggttca attcttcttt tagtgcatgc atttgttccc cataaaggaa
    24301 aatcatggcg aatttctcag gatcgatatg ttcactcatt tgcgcatgca tatttaaaga
    24361 ctctttccct aattccagca tatctgctaa cacagcgatt ctgcgtcctt ttgttggcag
    24421 atttttaaaa gtatctaata ccagtcccat ggctgttgga tttgcattgt agacatcgct
    24481 caaaatagct gcaccatttc ctgccgttag ccattcggtt ctgttttgag tgatagaaac
    24541 atgggcgagc ccttggcgta tctcaggtat ggacagtcca aagaagcgcc cgatcgtata
    24601 cgcaatcaaa gcattcgtta cattataact tccaggcagc gggatcgtaa atgtttctcc
    24661 ttctactaca aaatcggttt gtgcttttcc ttttgctgtc acttccgcat gaacatcgcc
    24721 atttccaata ccgaatgttg tgactgtttg tactaatgtt gccgttaaag gtgtcagcaa
    24781 cggttcatct gcgggcacaa cgagtaatcc gtctgataca agtccgtcag tgatttccat
    24841 tttggcttta gcgattcctt ctcgcgaccc aagattttct atgtgtgctt caccgatcat
    24901 tgtgattgct gcagcttttg gttgagccat aagtgaaagt tccgtgattt cattggcgtg
    24961 atccatgccc atttccaaaa cgatcttttc tgtatcttgg ggcatgtgca aaatcgtgta
    25021 aggaagacca atattattgt tgtaatttcc ttgcgtctta tatgttttgt atttttcaga
    25081 caagacagac gcggttagat ctttcgtggt ggttttacca ttgcttcctg taattgcaac
    25141 gacttctgcg cctacttttt ccaagtaata ggttgccaat tgctgcatgg ctttcaaagg
    25201 atctgctaca ggaataactg gaaaattttt tggagcttgt tcaacaggca agctccaaaa
    25261 tgaagcagat gctccgtttg tgatcgcttg ctggataaat tcatgtccat ctcgcgtacc
    25321 agcaagagga acgaataaac ttccttcggt tatcttccgg ctgtcaaatt cgacattggt
    25381 aatctttgtc agtgcagagc attcgctgcc cacggcttgt gcaatttctt gtattgttag
    25441 attcattgag atctctccta aaaaaggttg tgaaaaaagc gtttggccta aacggcgggt
    25501 tcgcatgact ctcacttcca agtaaatttc tctattcgta tttagaagct agaatgaacg
    25561 cggttacgca atcactccag cctcttcttt atttctcttt attcattttt ttcaaaaaaa
    25621 gattgacgct gatggtatcg attcattcct aactggatca gttcttcaat caaatcaccg
    25681 tatttcaagc ccatattttc ccataagagt gggtacatac tgaactccgt aaatcctggc
    25741 atagagttta attcattcag gaataattca tttttatttg tcaaaaagaa atcgcaccgg
    25801 ctcaatccgc ttccacctaa catcgtgtaa gctaacttcg cgtactcttg cgctttttga
    25861 taaacttctt ctggcacttc ggctggaatc tgcatttcga ttttattatt gatatatttt
    25921 gcttcataat catagaatgc tacgtctttt acgacttcac caggcaaagt cgtccgaacg
    25981 tcttcatttc ctaatacagc aacttcgatt tcgcgcgctt caattccttg ttcaacgatt
    26041 gctcgagaat catactgata ggctgttgct aaagcatttt gcagctcttc tcggttttct
    26101 gcttttgtaa tgccgacact agaacccata ttcgccggtt tgacaaacat cggataaagc
    26161 aaagaacctt cacattgatc aaatactttt ttaggatttt ctttccattg attcttaagt
    26221 actggtacat aaggaacttg cggcacacca gcagcttgta aaatatactt ggtcatgatt
    26281 ttatccatgg cacatgcact ggtcaatacg cctgcgccga cataaggcat attcaatgtc
    26341 tctaagaagc cttggatcgt tccatcttcc ccgtttggcc catgtaaaac tggaaaaacg
    26401 atggctcctt cttctttgat ttcgcccgga ttgatcactt ttcctgtaaa gccttcctct
    26461 gtctgtccac ttgggtccca tgaaagatgc aagacatctt tgctggcagg tttttctgtt
    26521 agtaatggac ctttgaccca ttgtccttct tttgtaataa atacgagttg aacttggtaa
    26581 taattataat aaatggcatt taaaactgaa aatgcggaaa gaatggacac ttcatgctct
    26641 gcgctgcgtc cgccatatag taaagtaatc ttcaaaagat tgtcctcctg tattctaaat
    26701 cgttcagtga tttactctca tattttagca caaaacaaaa ataaacgaat aacttattta
    26761 taaaaaaacg agccagcttc taaaagaacg agcgtcattt attttttgtc agctagatta
    26821 tttgtcccgt tttttataaa aagttatgat aaagaaaagg agggatgatg atggcgcaaa
    26881 caaatccacg cagccgcttg gcagaaatca ttcgagtgtt catcaattac aatgtagtgc
    26941 caaactttgt acagcaaaaa aatcccgaac aggtaaaaaa agcctttgaa gaactaggcc
    27001 ccaccttcat caagatagga cagatgcttt ctgttcgaga agatttgctt tcttctgcct
    27061 tcactcaaac attcaaaaca ttacaagata gtgtacctag cgacacattt tcaacggtca
    27121 aaaagacgat cgaaacagaa ctttctcttt cattatcaga tatattcgat gatttttcaa
    27181 aatcgccttt tgcctctgct tctatggggc aggctcatcg tgccaagctg aagaacggtg
    27241 acgcggtagt cgttaagatc caacacccta acatcgccga agagatccga cttgatcttc
    27301 aactatttga acgagcgatc cctttgatca aatacattcc tgaaactagc gtagttgatc
    27361 taaaaggggt actccaagaa gtaaaacgtt ccttgatcaa tgaaatggac ttcttaaaag
    27421 aaagtcaaaa tggcgaacaa ttctatcaaa agaacaatgg ctggaaagag atccgcagcc
    27481 ccaagattta tgacgctttt tgttcgaaaa aagtgatcgt gatggaagaa atgtctggta
    27541 aaaacttgaa tcatttaatg aacgccgaaa acaaaactga aacttttatc actggaattc
    27601 aaaacaagca gctgaaacaa gaagttgcaa agcttttagt tgagaacttt atgaaacagg
    27661 tgtttgatga tggttttttc catgctgatc ctcatccagg gaatctgcta ttccatgtac
    27721 taacaaaaga agaacagaca caagctagcc gaaaaactga aacagtccat gaaaaagagt
    27781 tcggttcctt tgcttttcga gcctccactt ctgcagaaga ccctgttgcg ccatatacca
    27841 tcaactacat cgattttggt atgatggggc atctttctgc aggacttagg caaaaactca
    27901 cacaagccgt tttagcgctg tatacgaaag atgcttatcg aatcgaaaaa gcggtccttc
    27961 gactttgtca gcaagaaggc tcgtttgatg aaagtcggtt ccaccaagaa ctgacaagtt
    28021 ttcttgaaca gtattatgat tctcctattg atgaaatcaa cttgcaagaa gtattcacac
    28081 atgtcgtgac gatttgtcat caaaataatc tgcagttcga tcgtgatatc acgcttttgc
    28141 tgaaagcatt tgggacatta gaaggggtga tccgcgtcct cgatcctgaa gtttccctca
    28201 tggaagtcgc ttcgcctttt gctcaacatt attttctcac tcatctggat gtagaagata
    28261 cattgaaaca aagcggatta gacttattgg aagggatgaa ggctgcgcct aagatccctc
    28321 aacagctcca ccatctatta gaaatgtgga cttcgggaca aggaaaagtc aatttggaat
    28381 tgaaaaagca agacaaatta ttgagcagga tcgaaagtat gatcaatcgg cttgttttcg
    28441 gtatgatttt agctgcattg atcgtagggt cctccctgct tgtccaagca gcacctgtag
    28501 aaaacgcaga ggtcgtttcc ttattaggga tatttacgta tgctattgcc gcattcgtca
    28561 ttattttttt ggcaatcgat gcattgatcc aaatgtataa gaagaggaaa aagtagaaaa
    28621 aggttgtgac agaaagaatc agcttcaaga aataagaagg ggttttacat aaattgtttc
    28681 tcaaattttt gtgaaaattc ggcttatttt tcgaagaggc tacttctgaa acaccgagaa
    28741 taaaaaatgt gaaaaagctg ttctgcaccc agtaataaga acaatcaaaa agggaacatg
    28801 gaacacattt atcctgtttc cctgttccct ttttgatgta ttcgagcata aataaaatac
    28861 cttcgttacc tactatcttc tgtacttggt aaaccttagt caattgcctt ttttaacgct
    28921 tgacgaaaag acatatgatc tttctccgta aagggttttg gaccctttgt ccttatgcca
    28981 gcttttctta actttccact atgataacgt tgttctagaa gcgtgccgat attcgcttcg
    29041 tcaaattctt ttccagagtg atggaacact cgcacaccct tcggcaatac aagtgaagct
    29101 aaaccataat cttgcgtgat caaaaagtca ttcttcttaa ccataccgac gattttataa
    29161 tccgcactat cagctccgcg gtctacgtag acgatttgga catgattagc gtattctttg
    29221 gtcgtataat ggtccacact ggtcacgata accacgtcaa gaccatagga ttctcctaat
    29281 tttatgacat cttcttttac tggagaacca tctccatcaa tgaatagcct cattcttttt
    29341 cgccttcttc cataaatgat aaacagttac taatccgctc agtaaaagac caacattgat
    29401 ccattttgcc aagataagca gttgtacact ttctatcggc actgattcat ggttcagcgt
    29461 gatcaataat agatggattg gctctcccat ttcttttgct aaaacaaaca aaagcgtcat
    29521 caataaaaac tggtggatca cactcccttt attttgagta gtatacttga caacgatccg
    29581 gcgatccatt ggaatagctg tatcagtttg ataatattcc ttatttagcc agctgcctgt
    29641 tactacgtgc cagaaactaa gcagaaagac acctatcgca aaaccatttg gaatcaagag
    29701 gaccaataaa gaagaactca gtccaagcaa ttgcatagcc aatggagacc atgttaaacg
    29761 tttcaaaatc catggtccga ctatttttgc tgcaatcaag cctgctgcat aaggcaaata
    29821 cataaaaatc gaaagttttt ctaatccgta cagtatgcca acataaaaac tgctaaataa
    29881 aaaaaggaaa ttccctagca ttccatttag aaaagtaaac agatttagcc agcttggcaa
    29941 cttccattcc ttcttctggt gtcctaaaac aaacacgaac aagatgaaca gcagcaagaa
    30001 accataaata gcaagatcaa aaagcctttc atcgaataac aatcgggcat ttctcaaaag
    30061 aaacaataag ccaaagaata tcaaaaataa aacaagctct cgcgtagcta ccagatttct
    30121 tttgatgtct ttgaaatcca gttcgtaacg cggataatga ctaaccgtat ggtaggccat
    30181 gacgtaaaaa agtgtataaa tagtaaagct tgctgcaatc tgagtcggtg caggcaactc
    30241 aatcgcttga tacaggagaa atagccagat agctgcaaat aagtactttt tcccatccat
    30301 attgataaaa ccttgcgtct tttcgtgaaa attgaccgtg gtattagcag gtacaagcca
    30361 tgctgcactc agccctagca aaacacttga aaaataaaat aaaggaaaat aaaatacgcc
    30421 taatattcca agcaatgaac cagcaccacc aatcagcaga gcgatcatca gcaacgtgta
    30481 actatctaga ccaaatcgta gactgttgac taaaaatagt ccagtcatcc gaaaggcata
    30541 aaacagcgta aatggcaaaa tcgtcagcca attatgcgag tctgttagtc ccaaaaataa
    30601 aagataagga ataaaaataa ccgtgttact taagataaag ggcgttgttt gtgtaaaatt
    30661 acgcccaaat cgtttgagtt tttcgaacat gacaatggct ccttaaacag tcttataact
    30721 aatctgctct tcgattttag cattaaaggg gaaataaacc aattttacag aatatattct
    30781 tgtaaaaaaa aatacagaaa gaagcataag cttctttctg catttttacg gtacttgatt
    30841 actcaagcag acaagccctt ctccacaaaa gtcttgcccg acacccctgc atccggcagg
    30901 tgataaagat agaaatcaat tatcctgtgc ctccaaaatc aattaggagc tctgtaaacc
    30961 agagtccttc tcgacccgtc gcataaattt actttaacac agttcctatg tttatgcgag
    31021 agcttttttc attttataat cgaccatttg aaatccttga ccgatatctg aaataagttc
    31081 ttctgtaatc tcaaatcctt catgcttata aacctcgatt gcttgatgat tcccctgatt
    31141 cactcgcaaa aagatctctc tttcctttgt ttgcgcagca agttcttcat accagcgaaa
    31201 aactgaagaa gtcaatcctt gaccacgtac tttatttagc aaatataatt tacttaaata
    31261 aatttttcca tttatctctt cataagcagt gtaaccgaca gtcttttctt cgctttttaa
    31321 taaaaagtag gacacacctt ctgtcagttc tttttgaatc tgtttttttg actgataagt
    31381 tttcagcatg taatccactt gtttttgacc aatgatcggc acaaaaactt ccggccatac
    31441 ttcttgtatg actgccatca gctcatccag ttcattttct gttactttta ttttttccat
    31501 acacccacgt ccttttttac attataaatg accttgcgct tgatggaata aaaagagcat
    31561 gatcatgcta tacacaccca ccgaccacgg tttcaagaaa atcacaatct gcataaattc
    31621 cctaaaagat aatttcgtca aaccagctac tagacagatc aagtcgtctg gcgcaaaggg
    31681 aagcagcaag gtaacaacta gtactttttt aatatctttc gtttttcttg tcagcaattc
    31741 atcaaatttc tgatactttt ttggcgataa aataagctgg acaaaggcta cgccatagta
    31801 acggacaagc aaaaatccga ttgcttcccc aatcactagg ccaatacaac tatataaaag
    31861 cccaaatcca ttgccaaaca tcagcatgcc cgctactgag gagatccctc ccggcaaaat
    31921 aggaatgatc acttggatca cttgcaacag aacaaatatc atcactgcat acttgccaaa
    31981 ttgctggata aacgcttgca aagacgtgct cgatcgaaaa atcccatgat gataagcata
    32041 aagaatcaac acaaagaaaa aaaacagacc aataatgggc atgatctgaa gacatttttg
    32101 taaaactgat ccttttttag acatctatat tccccacatc ttttttcatt tattaaaact
    32161 gcctcct
//
LOCUS       DACDSY010000012        30835 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDSY010000012 DACDSY010000000
VERSION     DACDSY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.012"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            87..1511
                     /locus_tag="IWK44_000525"
     CDS             87..1511
                     /locus_tag="IWK44_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9549081.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            1972..3081
                     /gene="pdhA"
                     /locus_tag="IWK44_000526"
     CDS             1972..3081
                     /gene="pdhA"
                     /locus_tag="IWK44_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP9549082.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            3084..4061
                     /locus_tag="IWK44_000527"
     CDS             3084..4061
                     /locus_tag="IWK44_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP9549083.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            4086..5381
                     /locus_tag="IWK44_000528"
     CDS             4086..5381
                     /locus_tag="IWK44_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HAP9549084.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            5389..6795
                     /gene="lpdA"
                     /locus_tag="IWK44_000529"
     CDS             5389..6795
                     /gene="lpdA"
                     /locus_tag="IWK44_000529"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP9549085.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            6957..7232
                     /locus_tag="IWK44_000530"
     CDS             6957..7232
                     /locus_tag="IWK44_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP9549086.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            7238..8005
                     /locus_tag="IWK44_000531"
     CDS             7238..8005
                     /locus_tag="IWK44_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP9549087.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            8173..10002
                     /gene="typA"
                     /locus_tag="IWK44_000532"
     CDS             8173..10002
                     /gene="typA"
                     /locus_tag="IWK44_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP9549088.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            10263..12035
                     /gene="recQ"
                     /locus_tag="IWK44_000533"
     CDS             10263..12035
                     /gene="recQ"
                     /locus_tag="IWK44_000533"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP9549089.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            12149..12451
                     /locus_tag="IWK44_000534"
     CDS             12149..12451
                     /locus_tag="IWK44_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP9549090.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            12454..13617
                     /locus_tag="IWK44_000535"
     CDS             12454..13617
                     /locus_tag="IWK44_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9549091.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            13643..17071
                     /locus_tag="IWK44_000536"
     CDS             13643..17071
                     /locus_tag="IWK44_000536"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP9549092.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            17092..18216
                     /locus_tag="IWK44_000537"
     CDS             17092..18216
                     /locus_tag="IWK44_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549093.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            18206..18643
                     /locus_tag="IWK44_000538"
     CDS             18206..18643
                     /locus_tag="IWK44_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9549094.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            18627..18965
                     /locus_tag="IWK44_000539"
     CDS             18627..18965
                     /locus_tag="IWK44_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP9549095.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            19084..19644
                     /gene="rsmD"
                     /locus_tag="IWK44_000540"
     CDS             19084..19644
                     /gene="rsmD"
                     /locus_tag="IWK44_000540"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP9549096.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            19641..20132
                     /gene="coaD"
                     /locus_tag="IWK44_000541"
     CDS             19641..20132
                     /gene="coaD"
                     /locus_tag="IWK44_000541"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP9549097.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            20122..21165
                     /locus_tag="IWK44_000542"
     CDS             20122..21165
                     /locus_tag="IWK44_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9549098.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            21324..22052
                     /locus_tag="IWK44_000543"
     CDS             21324..22052
                     /locus_tag="IWK44_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP9549099.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            22168..22413
                     /locus_tag="IWK44_000544"
     CDS             22168..22413
                     /locus_tag="IWK44_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9549100.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            22410..22628
                     /locus_tag="IWK44_000545"
     CDS             22410..22628
                     /locus_tag="IWK44_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP9549101.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            22764..23303
                     /locus_tag="IWK44_000546"
     CDS             22764..23303
                     /locus_tag="IWK44_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP9549102.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            23296..25569
                     /locus_tag="IWK44_000547"
     CDS             23296..25569
                     /locus_tag="IWK44_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP9549103.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            25607..26653
                     /gene="holA"
                     /locus_tag="IWK44_000548"
     CDS             25607..26653
                     /gene="holA"
                     /locus_tag="IWK44_000548"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP9549104.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(26742..26996)
                     /locus_tag="IWK44_000549"
     CDS             complement(26742..26996)
                     /locus_tag="IWK44_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP9549105.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(27396..27665)
                     /locus_tag="IWK44_000550"
     CDS             complement(27396..27665)
                     /locus_tag="IWK44_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549106.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(28135..28590)
                     /locus_tag="IWK44_000551"
     CDS             complement(28135..28590)
                     /locus_tag="IWK44_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP9549107.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(28728..30656)
                     /locus_tag="IWK44_000552"
     CDS             complement(28728..30656)
                     /locus_tag="IWK44_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9549108.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        10302 a         5345 c         6517 g         8671 t
ORIGIN      
        1 cttatgtttt gcaaaaaaag tttttctttt ttctcgtaaa ttcggtaaaa tcaaaagaaa
       61 aaatagacaa tagaaaagag aagcttatgt gggaaaaagt agagaaaaaa caaggaccga
      121 tttatcgtca aattatggaa cagattatga gagaaatcga aaacggaagg atcaatcctg
      181 gaagtcaact tgaatcagaa cgaaaactag ggcaactttt tggtgtgaat cgttccaccg
      241 ttgtccatgc attggaagag cttagggagc ttggggtact aacgagcaaa cgtggcagcg
      301 gatggtttgt taatcagact gaatggggga aattcgctgt tccaagaatc gattggcggc
      361 aaatgatctc tcctcgttat gagcagacgg actggtatga gcaaaaaata agagaagcaa
      421 agcagatcca aaaaacatcg tttcttgatt tatacgctag cgagatgcca gatgagcttt
      481 tgccgaattt tgagtttcct tcttattctc ttgaagaaat cttagcggaa gaaaaagaaa
      541 tggatctttt aggctatact ccgttacgta acaaaatcaa acggtatttg gaaagtaaat
      601 ttgaattggc attttccccc aaccagcttc ttgtgactgg tggcggtcag caagcaatct
      661 ttttgatttt acaaacgatt ctttcagctg gagaggggat agctgtagag tcaccttctt
      721 ttttttaccg attacctctt ttcaaagctt caggaacgcg tttatttggg ataccgatgg
      781 atgaagaagg gatcgactta gaaaaactga aagaatcgat ccataaaaat aaattgaaag
      841 cagtgcttat caacccgaat tttcaaaacc ctacaggaaa agtgatgagt caaaaacgac
      901 gagaaaagct agtagaattg tgtcgacagt atcggcttcc aattattgaa gacgatgtat
      961 ttacggatct ttcatttcta aaccaagaag aagtccttcc tatacgaacg attgatccgg
     1021 ataatgtgtt atacgtcggt tcgttatcta gggttttagg aaaaacaacg aaaatcggtt
     1081 gggtgatagg acctgagact tttatctcgc aactagctca agcgcaagaa atgatggagt
     1141 tttcgatgaa tatccttaca caaatcacag tggctaatgt atttaatgaa gcaattgatt
     1201 tgaaaatgca agatgtaaga aaaaagttga aagaaaaaag ccgactgata agcgcttggg
     1261 gagattcaca atcgtttttc aagcttcatt caattttagg tggttactat gcctggatta
     1321 cttgggatgg gaaaaaacta gaccccgaac tagcagaaaa agtagtagat atgggtcttg
     1381 gaattgcacc aagtctactt tttgggcaag atataaatgg aatcagaatc aattacagta
     1441 ggctggatga aaaccaaata ccgttgtttt attcaaagat gaaaattcta gaaaagtggt
     1501 taacagaata atttcacaaa gtaaatagat taaagaatat aacagcttgg atttaaattt
     1561 caagctgttt cttttttttc ggaaaaagaa tacgatataa agaaactaag aaaaaaccat
     1621 tatttgtacg tccgttcacg atgtgtaaat aaaataaatg tttgcaacaa aactgtataa
     1681 caccaacgtt tttaaataga aacagatggt tgtaagtttg attagaaaat agaaaaaatg
     1741 cagatgaaaa ataaaacgct ttccttattg tgattttatt cactaatgct gttaatatgc
     1801 ggattggcat aatttccttt catcgtgaaa atttgtgctt ttagcttgta tttctcataa
     1861 atccatgata atttattaat gaagacggtt aaatagtgat acagttttat tcactgtttc
     1921 aatcgcagaa tgaagagatt ttcttataga tgagagagga atgtgtgaca aatggcaaac
     1981 aaaaaagcac cgatcgactt ccaagcactg ctagatgaag tcaatgcaga tttcccagta
     2041 tatcaagcac ttgatcaaga tggaaacgta gttaacaaag atttagtacc tgatttatcc
     2101 gatgatgaac tcgttgaatt aatgactcat atggtatggt ctcgtgtatt agaccaacgt
     2161 tcaacagcgc tgaaccgcca aggacgccta ggcttttttg caccaactgc tggacaagag
     2221 gcaagtcagc tggctagtgc ttacgcattt gataaagaag atgttcttct tccaggttat
     2281 cgggatgtac ctcaactagt aaaacatggt ttacctttat ctcaagcttt tttatggtct
     2341 cgaggacatg ctgcaggtaa ttcttatccg gaagaattga aagctctacc accacaaatt
     2401 atcatcggtg cacaatatgt acaagcagcc ggtgttgcat taggtttgaa gaaacgaaac
     2461 aagaaaaatg tcgtcttcac ttatactggt gatggcggtt catcacaagg tgatttttac
     2521 gaagcaatca atttcgctgg cgcttaccat gcaaatgccg tattttatat ccaaaacaac
     2581 gggtatgcga tctctacacc tcgtgaaaaa caaactgcag caaaaacact tgcacaaaaa
     2641 gctgtcgcag ctgggattcc tggtattcaa gtcgatggaa tggatccgct tgcagtatat
     2701 actgtatcta aaatggcacg ggaatgggcg atcagtggaa atggaccagt cctaatcgaa
     2761 acactgactt atcgttatgg accacataca ctatctggtg acgatccaac ccgctaccgt
     2821 acaaaagaca cagatgacga ttggcagaaa aaagatccat tgatccgctt ccgtaaatat
     2881 ttgacggaaa aaggactttg gtctgaagaa aaagaagaac aagtcatcga agcaactaaa
     2941 gaagaaatca aagcagctat tgctgaagcc gacaaagtac caaaacaaaa ggtttcagat
     3001 ttcttgaaaa acatgttcga agtatctcca caatcaatca aagaacaaat tgcaatttat
     3061 gaagcgaagg agtcgaaata atcatggcac aaaaaacaat gatccaagca atcacagatg
     3121 ccttagcact tgaactagaa aatgacgaga atgtcgttgt ttttggtgaa gatgttggga
     3181 aaaacggagg agttttccgg gcaactgaag gattgcagga aaagttcgga gaagaccgag
     3241 tattcgatac tcctttagct gaatctggaa tcgcaggatt gagtttcggt cttgcgttag
     3301 aaggatttcg tccagttcct gaaatccaat tctttggttt tattttcgaa gcaatggacg
     3361 aagtagtagc tcaaatggct cgtacaagat atcgaatgag cggcactaga aatctaccca
     3421 tcacaattcg ttcgccattt ggtggcggtg ttcatacacc agaattgcat tcggataatt
     3481 tagaaggttt gatcgctcag tctcctggta ttcgagtagt gattccttcc aacccttatg
     3541 atgcaaaagg actattgatt tcttctatta gaagtaacga tccggttgtt ttcctagaac
     3601 acatgaaact atatcgctca ttccgagaag aagtgccgga tgaagcttac gaagttcctt
     3661 tggataaagc agcagttact cgtgagggaa cagatgtatc tattatcaca tacggagcaa
     3721 tggtgcgtga agcaatcaaa gcagcagaca atcttgcaaa agaaaatata tctgttgaaa
     3781 tcattgatct tcgtacagtt gcgccattag atgtagaaac gatcattcaa tccgtggaaa
     3841 aaactggtcg cgtagttgtt gtccaagaag cacaacgaca agcgggcgtt gccgcgcaag
     3901 tagtttctga gatctcagaa cgcgctatcc tttcattaga agccccaatc ggacgagttt
     3961 ctgcacctga tacagtcttc ccatttggac aagcagaaaa tgtctggttg ccaaatgcaa
     4021 aagatatcga agacaaagta aaagaaatcg cggaatttta agaaaagaag ggacgaaaca
     4081 aagtcatggc ttatcaattt aaattgccgg atatcggtga aggaatcgca gaaggtgaaa
     4141 tcgtcaaatg gttcgtcaaa ccaggagata cgatcaatga agatgataca ttactagaag
     4201 ttcaaaatga caaatcagta gaagaaatcc cgtcacctgt tacaggaacg gttaaaaatg
     4261 tgattgttcc agaaggtacc gtagcaaatg ttggggatgt attagtagaa atcgatgctc
     4321 ctggacacaa tagtgcaccg tcaactagtg cgccatcagc tgaagcacca aaagaaaaag
     4381 tggaaacatc aggttcagca agtgtcgttg aagctgccga tccaaataaa cgtgtattgg
     4441 ctatgccatc tgttcgtcaa tttgcccgtg aaaaagatgt agatatctca caagtaactg
     4501 caactggaaa aggtggcaga gtaacaaaag aggacatcga aaacttcttg gcaggtggtc
     4561 caagcagtgc accagcgaaa tcagaagcgc cagaagcggc agcaccgaaa gaagcagctc
     4621 ctgcagcaga aagcaaacca gctgcaccag caaaaccatt caagtccaac ttaggagact
     4681 tggaagaacg tgtagcaatg acgccaacac gtaaagctat cgctaaagca atggtcaaca
     4741 gtaagcatac agctccacac gtgacattgc atgatgaagt agaagtgtct aaactatggg
     4801 ataatcggaa acgatttaaa gaagttgctg ctgcaaatgg tacgaaactt actttcttgc
     4861 catatgtcgt gaaagcttta acagcgactg ttaagaaata cccagtattg aatgcatcta
     4921 tcgacgatgc aaaccaagaa atcgtttata aacattacta caatatcggt attgcaactg
     4981 atacggatca cggactatat gtaccaaatg tgaaagatgc agaccgcaaa ggaatgtttg
     5041 ctattgcaga cgaaatcaat gagaaagcaa aattagctca tgacggcaaa ctatctgcag
     5101 aagatatgcg caatggaaca attacgatca gcaatatcgg atctgtaggt ggcggttggt
     5161 ttacaccagt catcaactat ccagaagtag ctattttagg tgtaggaacg attgcacaac
     5221 aaccaatcgt aaatgctgaa ggcgaaatcg ttgtcggtcg tgtgatgaaa ctgtcattaa
     5281 gtttcgatca ccgtatcgta gatggagcaa ctgcgcaaca agcaatgaat aatatcaaac
     5341 gtcttttagc tgatccagag ctattaatga tggaaggatg ataaacaaat ggtagttgga
     5401 gattttgctg tagaattaga tacagttgta attggtgcgg gacctggtgg ctatgtagct
     5461 gccatccgcg ctgctgaaat gggacaaaaa gtagcaatca ttgaaagaga gtatattggc
     5521 ggtgtttgtt tgaatgtcgg ctgtattcct tcaaaagcac tgatagctgc aggacaccat
     5581 tatcaagagt cactagattc tgaaatgttt ggagtaacta gtgaaaacgt caaactagat
     5641 tttgcaaaaa cacaagaatg gaaagaaaac aaagttgtcc atacgttgac ttctggtgtt
     5701 ggtttcctat taaaaaaaca taaggttgaa acaatcgaag gagaagcgtt cttcgtagat
     5761 gatcacacac tacgcgtcat tcatccagat tcagctcaaa cttattcgtt caataacgca
     5821 atcatcgcaa caggttcccg tccaatcgag attccaggat ttaaattcgg aggacgtgtg
     5881 ttagattcta ctggcggatt agcattgaaa gaagtaccta agaaatttgt gatcatcggt
     5941 ggcggtgtga ttggtgccga attgggcgga gcgtacgcaa acttaggagc agaagtaacg
     6001 attcttgaag gctcaccaca aattttacca acttacgaaa aagatctagt aaaattagta
     6061 gaagatgatt tcaagaaaaa aggcgtaaca gtcgtaacga atgcaatggc gaaagaagca
     6121 gtcgataacg gtgacagcgt gacagtgaaa tatgcagtgg acgggaaaga agaatccgtt
     6181 actgctgatt atgtgatggt cactgtaggt cgtcgtccga atacagatga tatgggctta
     6241 gaacaagcgg gtgtcgaagt aggagaacga ggattgatca cggtggataa acaaggacgt
     6301 acgaacgtac ctaacatcta tgccatcggt gatatcgttc caggggcggc attagcacac
     6361 aaagccagtt atgaagcaaa aatcgctgct gaagcaatct ctggcaaaaa agtagctgta
     6421 gattataaag caatgccggc agtagccttc actgatcctg aattggcttc tgtaggtatg
     6481 acgatcaaag aagcaaaaga tgctggtatc gaagcaaaag catataaatt cccattttca
     6541 gggaacggtc gggcattatc gcttggtaaa acagaaggat tcatccgtct agttacgacg
     6601 atcgaagata acgtgcttat tggcgcgcaa atcggtggag tcggcgcaag tgatatggtc
     6661 tctgaattag ctttagcaat cgaatcagga atgaatgctg aagatatcgc cttgacgatt
     6721 catcctcatc catctcttgg cgaaatcgtc atggatgctt cggaattagc attaggttta
     6781 ccaatccata tttgatttga ataatacagg cgaaacgcaa ctgaaaaatc agttgcgttt
     6841 tgttttttag aattatcaat aatattatta tgtaaactta aaaatatgac aacttcttgg
     6901 tttattacca cttcctatgc tacactgaag aggaatatga aataggagag ttgaagatgc
     6961 aagaatatca gtatccactt gatttagatt ggacaacaga cgaaatggtc attgtaatga
     7021 agatgtggga agatctggaa cgtgcgaatg aaaaagggat aaacaatgaa gcttttttga
     7081 agacttatca gcagtttaaa actgttgtca aatccattgg tgaggaaaaa cgattgggac
     7141 gggaatttga aaaagcttcc ggttattctt tatatcgtac agtgcagcaa gcaaaaaaaa
     7201 atccaaacaa aactttgaga atgaacgggt gagataaatg gaacagatga ttgatcatat
     7261 caagtcctgg cttgaagaag ctggtgagaa aatcaaatct tctcaacaaa cattaacaat
     7321 ctcgcaaaaa tcaaatcgaa cggatctagt aacaaatgtc gatcgaaaca tccagaattt
     7381 tctaatcgaa aaaatccatt cctttgatcc agaggcgaaa atattggcag aagaagaagg
     7441 gtgtaatcaa ttatcggatc tcgatggaag agtctttatc atcgatccta ttgatggcac
     7501 cttgaatttt gtgattgaag gggaaaattt ttgtattatg ctcgctgttt atgaatccgg
     7561 cgttggaaag cttggtttca tttatgacgt gatgagaaat gaactttttt ggggtggaaa
     7621 aggaataggc gtgtataaaa atgatgagcc attgcctaga ccagcaaata aatccttatc
     7681 ggaaggctta cttggtgtga atagttatct tttcggtcat aataaatatg gaatccgaga
     7741 aattgcggaa agcagcatgg ggacacgtat gtacggatgc gctggtcttg aactagtggc
     7801 aatcttgaaa ggaaaccaca ttgcttatat ttcaaacctt agcccttggg attatgcagc
     7861 gggaaatgtc ttattggaag agtttggtat ggtaacaagc ggtctttttg gtactccttt
     7921 gacatttgaa aaaagggaat actatttagc agggacttct tgtgcatatg cagaaatcgc
     7981 agaccttttg aaaaaagaag gctgaaaaaa aggtagaaat cattcacaaa aatgataaaa
     8041 acgagcaaaa gattaatttt ctcgaaaaaa tgaaaacatt ttactatatt tcatgaaaaa
     8101 agtctgctat aattagcagt cgagtaaaat ttgccctgtc ataaagaacc aataaaaaag
     8161 gagttagtta cattgaatta tagaaacgat atccgtaacg tcgcaattat tgcccacgtt
     8221 gaccacggaa aaacaacatt agttgatgaa ctattaaaac aatcagatac attagacgca
     8281 catacgcaat tgcaagaacg tgcgatggac tcaaatgcgc tagaaaaaga acgtggtatc
     8341 acgattttag ccaaaaacac agctgtagac tataaaggta ttcgcgtaaa catcatggat
     8401 actccaggac acgcggactt cggtggcgaa gtagaacgga tcatgaaaat ggttgacggc
     8461 gttgtgttgg tcgtagatgc gtatgaagga acaatgcctc aaacacgttt tgtattgaaa
     8521 aaagcactgg aacaacatat cacaccaatc gttgtcgtaa acaagattga caaaccttct
     8581 gctcgtcctg agcatgtcgt agatgaagtt cttgaactat tcatcgaatt aggagcagat
     8641 gatgatcagt tagactttcc tgtgatttat gcatctgcac taaatggtac gtcaagcttg
     8701 tcagatgatc cagcagatca agaaccaacc atggcaccta tttttgatac aattattgaa
     8761 aaaatccctg ctcctgtcga taacagcgac gaaccattac aattccaagt ttcattactt
     8821 gactacaacg attatgtagg tcgtatcgga atcggacgtg tatttagagg aacaatcaaa
     8881 gtaggagacc aagtagcatt aattaaatta gacggaacag ttaagaaatt ccgtgtaacg
     8941 aaattatttg gtttctttgg actaaaacgt ctagagattc aagaagcaaa agctggagac
     9001 ttgattgctg tctcaggtat ggaagatatc tttgtcggtg aaacagttac tcctgtggat
     9061 catcaagatg cgttacctat ccttcacatt gacgaaccaa cattgcaaat gactttctta
     9121 gttaataatt caccatttgc cggtcgagaa ggtaaattcg taaccgctcg taagattgaa
     9181 gaacgtttga tggcagaact tcaaacagat gtatccttac gagttgaacc aacaaactct
     9241 ccagatgcat ggacagtttc tggtcgtgga gaattgcatt tgtctatctt gattgaaaac
     9301 atgcgccgtg agggatacga attgcaagta tctcgtccag aagttattga aaaagaaatc
     9361 gacggtgtaa aatgcgaacc gtttgaacgc gtgcaaatcg atacaccaga agaatatatg
     9421 ggtagtgtta tcgagtcctt gagcttacgt aaaggtgaaa tgcaagatat ggtccataca
     9481 ggaaacggac aaatccgttt gacattctta acacctgccc gcggtttgat tggttattca
     9541 actgaattcc tttcaatgac acgtggttac ggaatcatga accatacttt tgaccaatat
     9601 ctgccaatgc ttcctggtca aattggcgga cgtcaccaag gggcgcttgt ttcgatcgat
     9661 acaggaaaag caacgactta ttctatcatg agtatcgaag aacgcggtac agtcttcgta
     9721 gaaccaggta cagaagtata tgaaggaatg attatcgggg aaaacagccg tgataatgat
     9781 ttgactgtca acatcacgaa agcaaaacaa atgacaaacg tacggtctgc tactaaagac
     9841 caaacatctg tcatcaaaaa accaaaacaa ttgacgttgg aagaatcttt ggaattcttg
     9901 aatgatgatg aatattgcga agtaacacca gaaagcattc gcttgagaaa acaaatttta
     9961 gagaaaaatg ctcgtgaaaa agcatctaaa aagaaaaaat aatctgtaaa aggatgaaga
    10021 gagaaaccag cttgttataa aaaatagctg gtttcttttt ttgtccaact cttttctgtg
    10081 gagaagtcat ctttgtcaat ggacataaat tcagaaaata cgccgaaaga cgtttaaaag
    10141 acttttaaaa aacttatttc tagagagttt ttgtttcata ataagataat gtttcacgaa
    10201 aggagatcaa cttgtccttc gaagaaaatc gaagtaaaat ggcaagagaa aggagcggga
    10261 agatgaacga cttattaaaa caatactttg gttatgatga gtttagacct ggtcaaaaag
    10321 aaattatcca gaaagtcatt gatcaggaaa atgtattagg aatcatgcct accggaagtg
    10381 ggaaatcaat ctgttaccag cttccagctt tactattaga tgggcttact gtggttgtgt
    10441 cccctttaat ctctttgatg aaggatcagg tggacgcggc gaatcaatta ggtatacccg
    10501 ccacttttat caatagctcg ttagatggtt acgaaacagc tagacgcttc caagaaatcg
    10561 atagacagca gtaccgttta ctttatatcg ctcctgaacg ttttatcatg ccggatttta
    10621 tccaagcaat gaagcgatgg aacgtccgta tgatcgctat tgacgaggca cattgtatct
    10681 ctcagtgggg gcatgatttc cgcccaagtt atttacagat ggcgaaccag ctagatcaac
    10741 tgccaaatcg tcctgtaatt gttgctttga cagctacagc gactgtgcaa gtagctgctg
    10801 acataaaaag acttttaaaa attccagaaa acaaccatat tcagactggt tttgaacgag
    10861 aaaatctacg atttcaagta attaaagatc aaaaaaaaga acaatatcta atcgaatatt
    10921 taaagatcaa caaaaatcaa tctggtatca tttatgcagc aacgagaaaa gaagtagacc
    10981 gtctctatca tttgcttaaa aaattcgatt tttccgtggg tagatatcat ggtggtctaa
    11041 atgagaatga gcgaacagaa atgcaagaag cctttttata cgatcgcttg cagctgattg
    11101 tagcgacaaa tgcatttggt atgggtataa ataaaagcaa tgtccgtttt gttatccatt
    11161 atcaaattcc tggctctcta gaagcatact atcaggaagc tggtcgggcg ggaagagatg
    11221 gtctttccag tgaagccatc ctgcttttct ctcctcaaga tatccaagtc caaaagtttt
    11281 ttatccagca gtcccaaaga gaagaagggc aaaagcaaaa agaatatgaa aaattaaaag
    11341 cgatgacaga atacgtgtat attgagtcct gtcttcagca atacatccta aactattttg
    11401 gcgagacaag cagtccgtgc aaccgttgtg gtaactgttt agatgacaga gaaatagtag
    11461 aagtaacgac agaagcacag atggttcttt cttgcttaaa acgtatgggt gagaactatg
    11521 gtaaacaaat gctgatgaaa gtcttagcag gatcgaaaga acaaaagctg caagctttag
    11581 gttttggaca tttatctacc tatggactga tgaaaaacca atcgcaaaaa gaaacgatgc
    11641 agttaattga atatttgata tcgaatggct atcttctgac catcaatggt gagtatcctg
    11701 ttttgaaagt aacagaaaga gggatacaag tattgaaagg acaagaatct gtttatcgaa
    11761 aggaacccaa gaaagtccag cagctatcgg atgaagaaac cgatacacta tttgaagtgc
    11821 tgcgggaact gcgaacggat ctagcatcag aagcaggcgt tcccccgtat gtcgtatttt
    11881 cagattccac attgaaagaa atgagccgaa tacgtccatc aagcagattg gagatgcttc
    11941 agataaaagg agttggccaa agcaagcttg ataaatatgg cgaagccttt ttatctcgga
    12001 taaaaaatgc ggactctaac gttctggata agtagaaaga acgaatacgt aaaaatgaat
    12061 aaaaacaact ggttaaatct gttttttttg ctccttatga aaatcaaata tgttataatt
    12121 aaggaattag aatgggaggt aagcacagat ggattctttg tcgaaaaaac ttgcgatcca
    12181 agtattaaat gatgaagcag aacggatcaa acgattaatc cgaaatcaaa acaacagtct
    12241 ctgtatctca caatgcaaag ccttcgaaga agtggtagat acacagatgt acggcttttc
    12301 ccggcaagtc ggttttgctg tccgtatagg agtaatcgga aagaacgagg gccagctgat
    12361 gttaagtgag ctagaacgag aattgaatca gttatatagt gaggtttacc aagaaattta
    12421 tgataaaaaa gaagttggca aggaggaata gacttgccta agaaagtgaa aaaaaggcat
    12481 ctattagact atagcatctt gattccctat ttaattttat gtgtattggg attgatcatg
    12541 gtatatagtt ctacgtcgta tctattatta gaaaacggac aaaatccgtc cgcatcagtc
    12601 attaaccaga gtattttttg ggtgcttagc ttgattgcta tcgcactact ttataaaatg
    12661 aaaacagatg ttttgaaaaa ccagcgtttg atcatggcag ccatcgcagt tttgacgatt
    12721 ttgctgctga ttgttgtgtt ttttggtaag gagatcaatg gggcgaaagg ttggctgcaa
    12781 attgcaggat tctcgatcca acctgccgaa tacctaaaaa tcatttctat ctggtacctg
    12841 tctctgactt tatctaaaag acaaaacagt gtacaaaaag attttcttgg gactgtgaaa
    12901 cgtccacttg caatggttat cggacttact gcattagtcg ctattcttcc cgattttggt
    12961 aatgcggcag ttatcttttt gattattttg gttctgcttt tagccagcgg agtcaattat
    13021 gtgtatactt tgattgtagg agtaggaggc ttttgtctaa gtacttttac gatctggctg
    13081 atcaatatca caaacggaaa gatcttaccc ggacgtttac agtatatcta taatcgattt
    13141 gctatttacc aaaatccttt ctcagacgaa ttaaataaag ggcatcaact agtcaacggc
    13201 tactatgcca tgttcaacgg aggactattt ggcaggggat taggtaacag tatccagaaa
    13261 aaaggatttt tacaagaagc acaaaccgat tttatttatg caatcgtagt ggaagaactg
    13321 ggagtgatta tggggatttt gatattagcg ttgctattct ttatgattgt gcgcattgtc
    13381 cttgtaggta tccgttcaaa agatccgttc aattcgcttt tgtgcattgg gatcggcgca
    13441 atgtttttga tccaagtatt tgtcaatctt ggaggaatta ctggcgttat tcctttgaca
    13501 gggatcacgt tcccattttt aagtcaagga ggctccagtt tgctgatgct ttcgatttgt
    13561 gtcggctttg tcctcaacat cagtgcggat gaaaaacgca agagcttagg tttataataa
    13621 aaaaagatgt aggagtggtt cgatgaaaaa agttttagtt gctaacaggg gtgaaatcgc
    13681 tgtacgtgta tttcgtgctt gtacagaatt aggaattaaa actgttggaa tctatgcaga
    13741 agaagatgaa tattccgtac accgtttcaa agcggatgaa gcatatttag taggacaagg
    13801 aaaaaaaccg attgatgcgt atctagatat tgaagggatc atttctatcg cgaaagaatg
    13861 cggagctgac gctattcatc cgggttacgg tcttttgtcg gaaaacttga attttgcgca
    13921 acggtgcgaa gaagaaggca tcatttttgt tggacccaaa ctgcatcatt tagatatttt
    13981 tggagataaa atcaaagcaa aagctgcagc tatcgaagcg gggattgctt cgattccagg
    14041 cacagatgga ccaatcgctt ctatagacga tgccttggag tttgcgaaac aatacggtta
    14101 tccgattatg atcaaggcag ctctaggcgg cggcggacgc ggcatgcgtg tagctcacga
    14161 tgaaaagagt gcaagagagg gctacgaacg tgcgaaaagc gaagcaaaag cagcctttgg
    14221 tagtgatgaa gtgtatgttg aaaaatacat cgctaatcct aagcatatcg aagtacagat
    14281 tctcggggat acgcatggaa acgtcatcca tctttttgaa agagattgtt ctgttcagcg
    14341 ccgtcaccaa aaagtagtag aagtagcgcc ttgtgtctct atgaatgaac agcaacgtca
    14401 aaaaatctgc caggcagctg tccaattgat gaaatatgtc gggtatgtca acgcaggtac
    14461 agtagaattt cttgttgaag gagatgattt ttactttatc gaagtcaacc ctcgcgtcca
    14521 agtggaacat acgatcacag aaatgattac agatattgat atcgtaacaa cgcaactgtt
    14581 gattgctcaa ggtttggatc tgcataaaga aatcggattg cctcagcaag aaggaattaa
    14641 attaaatggt tcagccattc aatgccgaat cactacagaa gacccgttga ataacttttt
    14701 accagacaca gggaaaatcg atacgtatcg ttcacctggt ggttttggcg tacgtctgga
    14761 tgtcggaaat gcttacgcgg gctatgtagt gacaccgtat tttgattcat tgctagtaaa
    14821 agtatgtacg catggtgcta cctttgaaac agccattcag aaaatggaac gttgcttgaa
    14881 agaatttaga atccgtggag tgaaaacaaa cattccattt atgctcaatg tcatcacaca
    14941 tcctgaattc caatcaggaa atgcaaaaac aaccttcatt gatagtacag caacattatt
    15001 tgaatttcct cgactacgag accgcggaaa caaaacgatg aaatatatcg gtgaaattac
    15061 tgtgaatggt tttccaggta tcgaaagtgg cgaaaaacca ttttatgaag aaccacgtat
    15121 gcctaaagat ctaatcacaa gaactgatta cgtaacggct aagaatgtat tagatgcgaa
    15181 gggtgccgat gctttggtcg aatggatcaa aggacaggaa aatttgttgt tgacggatac
    15241 gacatttaga gacgcccatc aaagtctgct agcaactcgt gttcggacaa aggatttcaa
    15301 gcagattgca cgtttgactg gagaaggtct tcctgaactc ttttcaagtg agatgtgggg
    15361 gggagcaaca tttgatgtgg cttaccgctt tttgaatgaa gatccttggc aacggttgag
    15421 aaaaattcgt tctttaatgc ctaatacgtt gctgcaaatg cttttcagag gttcaaatgc
    15481 cgttgggtat tctaattatc ctgacaatgt attggtggaa tttgtcaaag aagcagcagc
    15541 gcaaggaatc gatgtatttc gaatctttga tagtttgaac tggactccac aaatggaaaa
    15601 aagtatccaa gctgtacggg atactggtaa aatcgcagag gcagctattt gctatactgg
    15661 cgatatcaac gatccaagtc gggcaaaata caatgttcag tattataaag acatggcaaa
    15721 ggaactagaa caactgggtg cgcatatcat tgcaatcaaa gatatggctg ggctgcttaa
    15781 accgcaggca gcttatcgtt tgattagtga attgaaagaa acgacagatc tgccaatcca
    15841 tctgcatact catgatacca gcgggaatgg aatcatcact tattctgctg cttcaaaagc
    15901 aggagtagac atcgttgacg tggcaatgag tgcaatgagt gggaatacta gtcagcctag
    15961 catgagcagc ttgtattatg cactggtcaa tggtccacgg ctgccagaaa tcacgattga
    16021 aaatgcacag aaattaaacc attattggga agatgtgcgc atgtattaca aaccgtttga
    16081 aaacggcttg aatgctcctg aaacagaagt atatatgcat gaaatgccag gaggacagta
    16141 ttcgaatcta cagcaacagg caaaagctgt cggattaggt catcgctggg atgaaatcaa
    16201 acaaatgtat catactgtca acttgatgtt tggcgatatc gttaaagtaa ctccatcatc
    16261 taaagtagtt ggagatatgg cactatttat ggtccaaaat gatcttacag aagaagatat
    16321 ttatgaaaaa ggcgaaacac taagtttccc tgaatctgtt gtgactttct tccaaggaga
    16381 actagggcaa cccgttggcg gatttcctaa aaaattgcaa aagattattc ttaaaggcag
    16441 acctgcattg aatgaacgtc caggtctttt ggctgaatca gtcgacttca acgaagtaaa
    16501 aaaagaattg gcagaaaaaa ttggctatga accaaaacaa gacgaagtat taagctattt
    16561 gatgtatccg caagtctttt tggactatca aaaagcatat aatcaatttg gcgatgttac
    16621 attactggac acaccaacat ttttccaagg tatacgtttg ggtgaaacaa tcaatgtaca
    16681 aatcgaacgc ggaaaaacgc ttatcattcg tttagatgaa atcggagaac ccgatatcga
    16741 agggaatcgc gtactgttct tcaatttgaa tggacaaaga cgtgaaatca ctatcaaaga
    16801 taattcgatc atcagtacgg tccaaacccg attgaaagct gaaccgacaa accgagaaca
    16861 aattggagca acgatgtctg gttctgtttt ggatgtatta gtgaaaaaag gcgataacgt
    16921 taaaaaaggt gatacgttga tgattactga agcgatgaaa atggaaacgg ccatcgaagc
    16981 acgttttgac ggagaagtcg cacatgtata tgtgtcttca ggagacacta tttcgtctgg
    17041 tgaccttttg attgaagtga ctgagaaata ataagcgaaa ggtggggaaa catgaaaaga
    17101 ctgggtttgt tttggctgat tctgctatta gttattgtgg cattctacat ggaaccagtc
    17161 ttgtttccgc caaaacttga agtgaaggaa aactctgatc aacaaatatc aaaccaaatg
    17221 acaacgtgga aataccagga gcttaaagca actggttttt ctacttatat cggaaaaccg
    17281 atcgatcagc tggaaaagga ttttggccaa gcctatgatc gattaactag cggatttggt
    17341 tttgaaacaa gatattatga aaataaagct agtcatctca catttgaagc gaacatccaa
    17401 gacagcagag tcacaactgt taaagtactg caaacagggc ctgctgatat cgcgccgttt
    17461 tatttaggga tgacgatgca agatcttacg aaaatcacta ctatctatac taatttcact
    17521 tttgagtacc atgatacaga agttggaatc gaactaatgg aagaggatat gaattatcgt
    17581 ccgctaattg cttttgataa cgacactttt gctattttat tttttgatca aacaacaaac
    17641 ggcctatttt cagttgtgta tttagataaa gatagtcttt tgaaactatt gccatatcaa
    17701 gtgtttggtg aaggccttcc acattatcaa gcagataaat cggctgattg ggaatcaatc
    17761 aatcgcgtta aagaacgaaa aagtacgact ttattaaata tgcttagaag agaagacgaa
    17821 cttcccatat acaatcaaac acttgccttt caaaaccaca gtcagctgct actacatgat
    17881 tttttgacaa ggcctgagga aatattgtca gaggaacgta tggctgattg gcaaaaatcg
    17941 ctaggcagtg ctcaaacgac agtgaaattc tcactgacga atgatgaatt ggagaagtta
    18001 atcagtaaac aaaaattaga aaatactagt gggctattta ttcatccggc gattgacgct
    18061 actttttcgt ttttatattg gtacagcgat ccttacaacc atgaccgttt catggacccg
    18121 ggaacggatt ctgtaggcat tgctttttct aaagaaaata tgttagtctt attgcaagaa
    18181 gaaaacggag aaagtagtga tagcagtgat cgttaacgaa gagctgtttc aactggagga
    18241 tcaatgcctc caagtcgctg aaatgataaa aaagagtaaa acgatggctt cttatcttga
    18301 acataaacag tcaatggaag aatctgcaaa agtttgccag ttgaaagaag cgttcttgtc
    18361 taagaaagaa gcatttgaaa atgtagcggc atatggtgag tatgcacctg attatcgtga
    18421 aaaaaaacga gctgttcgaa aagcaaaaag acagcttgat atggatgagc atgttgcagc
    18481 tttccgatat agtgaaacac agctgcaaag tctgttggat caaattgtcc aagtacttgc
    18541 cgaagaagtt tctaatgaaa tcaaagtaga tgcaggtagc ccgttttttg aaacgaaaaa
    18601 acactttgga tgcggaggta attgtcatgc aattagacaa tagtgaattt gaaataacaa
    18661 aacgacgcgg gctgatcgta tgggtctaca gcttaaaaca attaaagaat ctgaaaaagt
    18721 tcggcttgtt gcattatgtc tcaagaagaa tgaaatatgt cgtgctttac ttaaatgaag
    18781 agacattgga agaaacagaa gaacgaatcc gtaaattaca ttttgttcga aacgtagaac
    18841 gttcttaccg accggatatc gaaatgaatt ttgccgaaaa aataggaaac aaaacagtat
    18901 taaaagacga aggtttcgaa attgaagagc taaatacaga aatccgatta gctgaacatc
    18961 tttaaaaatc tcttatgagc agtttttaaa taaaaaataa cttggataac gatcgatttt
    19021 tgatcattgt ccaagtattt ttttggtata ctagtaccag taccatttct aaggagtgag
    19081 tagatgcgga taatatcagg aaattatggc gggcgccgtt taaaaagttt agcaggggcg
    19141 aacaccagac caactacaga taaagtaaaa gaatccatat tcaatatgat tggaccatat
    19201 tttgatggag agacagtatt ggatctgttt gcaggaagcg gtggtttagc tatcgaagca
    19261 gtatctagag gctgcagtca tgcggtatgc gtagacaaaa attaccaagc gctaaaaatc
    19321 atcaaagaaa acatcgaaat tacgaaagaa ccagagaaat ttaccacaat aaaacttgat
    19381 gcggacaaag caatcgaacg gctagcagcg gagaaacaac aattcgacta cttgtttctt
    19441 gatccgccat atgcaaaaca aaaaataatc gatcaaatcc ataaaatgga agaactggga
    19501 cttttttcta aaaccgcagt tatcgtatgc gaaacggata aaacagtcga attgccagaa
    19561 actgtggctg gattccatca aataagaaaa cagatttatg gcataacggc cgtaactatc
    19621 tatagaaaag agggaaatca atgaaacgtg cattattccc aggaagtttt gatcctttta
    19681 caaaaggtca cttagatacg gtggaacgtg cggctaaatt attcgatgag gtagtaattg
    19741 gtgtttttat caatacttca aagaaaagtt tgtttccccc agaagaaaga atgacattga
    19801 tcacaaaagc tgtttctcat cttcctaatg taaaagtcat gcatcaagaa aatcaattga
    19861 cggtagaaac agcaaaagag attggtgcag atgcgttgat cagaggcata cgaagtatca
    19921 aagactttga atatgaacga gagattgctc agatgaatca tcatctatat gatgaattgg
    19981 aaactgtctt cttattagca aaaccagaat atagtcacgt gagttccagt attttaaaag
    20041 aagtactgca ctttggcgga gatgtgagca gctacttacc gccagtgatc aacgaagctt
    20101 tagctggaaa gcgtgaatca aatgaaagct aaacaatggt tgaaaacagt actgattatc
    20161 atatttggaa tgttgatcat tggatttgta gtagttccct tgccgtatta tgtagaatca
    20221 ccgggagcca cgatcgatct gaaagaacta attacggtca atgataagga agataaaggt
    20281 tctgggtctt tctctcttac ctctgttggt atcaggcaag ctacaggttt cttagcttta
    20341 aaagctaaaa tgaccccttt tgaagaattg atcagtgaag aagaattgac tggtggggcg
    20401 acaaatgaag aatataatca aatgcaacag tattatatgg attcttccca aaatgcagca
    20461 atcgaacaag cgttaaaact tgcagacaag ccttataaaa tggaattcaa aggcgtatat
    20521 gtcatgagtg tagagaaaac atcgaatttc tttggcaagc tttcagttgg agatacagta
    20581 acaaaagtag acgggaaaag ctttcaaagt acagaagcat ttatggatta tgtaaaaagc
    20641 caaaaagttg gacaaacaat cgaaatcagt tatttgcata atggtgaaga aaaaacagct
    20701 tctggtgatc tgatcgagtt accaacagat aaaaaggcag gaataggtat cacgcttact
    20761 gaccataccg agatcgaatc agatacagat gttcgtatta attctggctc tatcggtggg
    20821 ccatcagcgg gattgatgtt cactttagaa atctatgaac aagtcactgg taaaaacttg
    20881 cgacatggta agcaaattgc aggaactgga acgatcaaca gtgaaggaga agttggccga
    20941 atcggaggaa tcgataaaaa agtggcaagt gcggataaag ctggcgtaga gattttcttt
    21001 gcaccagatg atacgatttc agacgatgtt aagaaagaat atcctgaaat aaaaaccaac
    21061 tacgaagaag caaaagctgc cgctaaaaaa ttgaattcct caatgaaaat cgtaccagta
    21121 aaaacagtac aagaagcgct tgactatcta gagaaaatga aatgaaacta aataattaaa
    21181 gaggggttgt gacacgagtt ctgtcacaac ccctcttttt cgtcaaaaat cttcacgata
    21241 aaaataaaaa caaaagaaaa aattgacgta attagatgtt gaagggacga ttttgccaga
    21301 gctcactgtt tcttcaattt taaatgaaag gagtaatgaa tgatttttac tggtcattct
    21361 ttgaaaaaag aacaatgata gaaaaaatca gcaagaaaat actagcttca cccaaattga
    21421 tcatttggat gtctatgata gttcttatct tttgtatcgc aagtggtttt ttattatcaa
    21481 atttgctatt ttatagagaa acagaagaga ctgctgcaaa agaaatactt tcttcgttta
    21541 gttccagtgg agaagatcca tcagctgaag gaaacgatgt agaagaggat gctcgtccaa
    21601 aggtcatgta caccgatatc aaaggaagtg tgaaggagcc tggtatctat tccttttctt
    21661 cagaagaacg ggtctatgat gtactaaagc gtgcaggtgg attattagaa gaagcggata
    21721 gcgatcgaat caacttttct gcgaagatag aagatcagca agtcctatat atccctgctg
    21781 taggtgaaga gctgccagaa catcttgatc agttggcatc ctcggaagga aaacagccta
    21841 cagcagattc cgagcctccc aaaataaata tcaatacagc cactccatca gaacttcagc
    21901 agattcctgg gatcggatca gtcaaagcgc aagaaatcat tcgatttaga gaagaaaacg
    21961 gatccttcca gaaagtggaa gacctgcagg agatttctgg tatcggtgaa aagacagttg
    22021 aaaaactaaa aaacttcgtt acaattaaat agaatgattc aaaatcgact ggcacgagca
    22081 ataaatggac gctaatggat actaagaata atccaattct aagagcttgg caggacgagg
    22141 aggtagtatt tttggatagg aaatagcttg attgttcgca aagaaacatt aaaaaaacca
    22201 atgctgaatg tctatctcca aaataaaata tctgggattc atattatgaa tacggctgtc
    22261 agcggcaata acagtcaagc tctacgagaa cgctttgcca aagatgtact tagctataca
    22321 gcagataaag tatttattct cattgggacg aatgatctag cagagcataa acaactttct
    22381 aaagaaacgt accaaaaaat ctgcagtgga tgatagttca gctgaaaaaa gactatagtt
    22441 ctgatcatgc tttctttatc acgccaccag cagtcgatga gacaaaacaa aaatatcgga
    22501 ataacaagtt gatcaaagaa tatatctctg ttctcacaga agtagcaaaa cgccatgatt
    22561 gtgaagtatc gatttttata cggaatttct tgaacaaatg gattcgaaga atttttataa
    22621 tgggctaatg gatgacggac ttcatttcgg cagtgaaggg taggatcttt tgtcagacag
    22681 gatgattcgt tatatttaaa gaatattttg tttaagcaat gagtactttt taatcaactt
    22741 gaaaaaacgg aggaatataa aacatgttag aaaaatcttc aaaagcatgc gatacgaata
    22801 aagaaaaagt tactgataag tcacataatg aacgcattcc ttgggaccag tactttatgg
    22861 ctcaggcagt actgttatct ttacgtagta cgtgtacacg tttagaagta ggagcgacac
    22921 tcgttcgaga aaaaaggatc attgctggcg gctataatgg agcagtcagt ggagatgtcc
    22981 actgcataga tgaagggtgt tacgtcgtgg atggacactg cttgcgaacg atccatgcag
    23041 agatgaatgc actcctacag tgtgcaaaac ttggtatccc gacggaaggt tcagaaatct
    23101 atgtcacaca ttttccatgt ttggcctgta caaaggcttt gttgcaagct ggtgtgaaag
    23161 ggatcaatta tctccatgac tatcgaaatg atccttatgc ccaagcgttg atcgaacaat
    23221 taggtgtatc tgtacaccaa gttgctttag actcacagca tttttcaaaa cttcaaaatg
    23281 aattaacagg aaaacatgtc tagcgtttta gaaaggcatg ttcgaaatcg gttgattttt
    23341 ccggcaatta cgtcgatgat tatctgtttg ttgtattaca ctgacaattg gggttatcgt
    23401 ttgcttcttg ccggaatttt tttatggttt tcgctaaaag aaggacgttt tatcgcgtac
    23461 ttgtgtattt tgtgtagcat aacggtatcc atttcttata ggctacaaga gtattcattt
    23521 ttagaggaaa aaacaacatc tgcgcaaatt actgtattta cagatacgat tcgtgcaaat
    23581 ggtaattttt tgacaatgga aggaaggtta tcagagaata aacaaaagct ccagctttct
    23641 tatcaagtaa acacagaagc agaattgaaa acatggctta acttccgagg aaagggaata
    23701 gaattgactg ctgaaggcga gttcatctct ccagcaaaaa agcggaatct ttataatttt
    23761 gaccaagaag cttacttcag gaaccataga atttctggac ggttccaaat tcaaaagatt
    23821 gaacagatca ccatgcaaaa tcactggcga aactggcttc agagaaaaag aggatctttt
    23881 atctcctttg tccaatctga atttccgcaa aaaacaagtg tatatattaa ttctttatta
    23941 tggggctata aagatgcagg attcagagaa ccagaagaaa tttttaggga gagtggtttg
    24001 ctgcatctgt tcagtctatc gggtctacac atccaatttt atttagggtg gctgtattat
    24061 ttatttcgac gtatcggatg gccactacac gtcagtatta ttccgttgag tctactaact
    24121 atctgttatg tttctattgc aggaagttct atttctgttt tgcgtgctag tttgctgttt
    24181 ttattgcgat tgctagtcaa attgctgaat aagcattatt ccaccatgga cttatttgca
    24241 gttgtctttt ggcttctttt actatttgat cctcgatttc tttttcatgc gggtggacag
    24301 cttagttttt tcatgagttt tttgtttatc ctgatttctt ttgatggaga ttggttgaaa
    24361 aaaacggcct ctcacgtcct gtttactatg atcacgatgc cgttgattgt atggcatttt
    24421 tatgagtggc ctattttagg tagtcttttt acattactga ttgcaccggt attcaagttt
    24481 ctttttcttc ctggtgtgtt atttctcaca ctcttcaatc gctggctgcc ggaaacttta
    24541 cttttgctga tagaagaggg attaatctta tttgagcgaa taatcgattt ttcttcaggg
    24601 tcgtctctca ttataggacg tttgcccctt gtgctatctg ggatgtatat tttcggatta
    24661 ctgatttgta tggataggtt gaaagaacaa cctgtgaaac acttgtttta tgttggtctt
    24721 ttttctttta tttttttgac tttgccattt ttacgctttt cttcaacgat cgcttttatc
    24781 gatgtcggtc aaggagacag tatctttttg caaagtcctt ttcgaaaaga aacgattttg
    24841 atcgatactg gaggtcagtt acactttgaa agagaaaagt gggcgcaagg tatgatacgt
    24901 cctcctgctg aaagaaatat cgttccatat ctaaaaggtc aggggataag tgtaatcgat
    24961 aaactcatct tgacacacga cgatacggat catgtgggag aattacccac gttatctcag
    25021 cattttacta tcaaaaaaat ttatatcggt tggggcgctg gacgaaatga gccattacaa
    25081 aggcatttat ctgaagctca aaaaaatggt actgagatca ttgaaatcaa acatggggac
    25141 aggatcagcg gttattatga tctttatgta ttgactcctt ttgaaaaggg agagggaaga
    25201 aatgaagatt cgattggtct atggatggaa tataacaata ggcgcttttt atttttagga
    25261 gatttgaatc aagcaatgga aaaacaattg cttttgattt atcctaatct gaaagccgat
    25321 gtagtcaaat taggtcatca cggtagccgt acgtcaagta acacagattt tctttcccat
    25381 attgaagcaa aacatgggat tatatcctgc ggcgtaaata atcgttatgg tcacccgcac
    25441 agcgaagtat tggagacact agaagaaaat cagataaaaa catggcgaac ggatcaacaa
    25501 ggaatgatca cctatcgctg gcatccagtt tttcatcctc atgggctagt ggaaacaatg
    25561 atagactagt aattctcatc tgctcatggt aggataattt ttgaaagtgg tgagtaattt
    25621 gagtatacaa gcagagttac aaaaaatacg taaagaaaaa cttgcatctt gttacttgat
    25681 tttaggaaca gaaaagtttc tccaagatca agttcggaca gaaattgtca aaaaaatcca
    25741 attagagggc gaagatgact taaatttttt aagttttgac atggaaaatg caagtttaga
    25801 tgaggtagta gctgaagcag agacgttgcc gtttttcgga gatcaacggt tagtattcgt
    25861 agagaatcct tattttttaa caggtgaaaa agtaaacaat ggaatcgaac aaaatactga
    25921 tttattgacg gcttatttga aagaaccatt agaatcgact gtattagtat tctttgcgcc
    25981 atatgaaaag ttggatgaac ggaaaaaagt aacgaaacaa ttgaaaaaaa cagcaattat
    26041 catcaatgtc caacagttga atgaaaaaga agtacgtcaa tatctgatga ataccttaga
    26101 gaattcctca atcaaaatgg aacgaagtgc aattgattta ttcttgaggt tgaccgactt
    26161 agatttatcc aaaatgatga gagagttaca gaaattggtc ctttatggac aaaatcaaca
    26221 gcaaatcacg gtaagagaag tagaagaatt agttcctaaa acgttagaac ataatatttt
    26281 tgacatgacc caatatattt taaaagggaa gacggaacaa gctttgcgtt tatacgaaga
    26341 tttagttatg caaggggaag aaacaatcaa aattaatgcg attttgcttt ctcaattacg
    26401 tttattttta cagacaaaat ttcttgtaaa gattggttat cagcaggcaa atattgctga
    26461 aacattgaaa atccatccat atcgtgtaaa acttgccatg caggaagtaa aaaaattcga
    26521 tgaacatttg ctggtacagt tgtttgatca attagttgag atggattatc aaatcaaaac
    26581 gggacaaatc gaaaaagaat taagttttca actattcgtt ttgcgcacag ggcagttgtt
    26641 aagaaatcga taagaagaaa gacgagggca aagcatgcgg gaaggtcgtt tgtttttgtc
    26701 ctttttttga caaataaaaa aatcggccga agccgattct tttatttagc gatttttttg
    26761 cttaaacgag atttgtcgcg gttagctttg tttttgtgga ttagaccttt tgattcagcc
    26821 atatcaatag ctttaacagc ttctttatat aacgcatcta cgttgtcagc acctgaagct
    26881 acagcatctt caaatttttt gattgcagta cgcatagcgc ttgtttgaga tgaatttttc
    26941 acattagcgt tttcgcttgt acgaacacgt ttgattgcag attcaatgtt tggcattagt
    27001 ttcacctccg ataacttaag ttcttacgcg tgttaccgca taattcaaca ttcattatta
    27061 tacctaaagg cagcgtgctt tgcaatatga acatgacaag tttttttcct taacagtctt
    27121 ttattaggat tttttttcag taaaaacgga tagtacaagc tgaaaaaaat caaaaagacg
    27181 ctattgtggg aggcaatagc gtcaaaccaa actagatttt tatttatcta tttaaacaaa
    27241 gtttgaatgg gaatgtttgg tactaagtta ttatataaca aagagaaccg tttgcaaagt
    27301 tttttttgaa aagggatacg attccctgtt gttctttgtt tttctattaa gaaactgcag
    27361 ttattttata accagtatcc tttgatcagt aaaggctatt ctggtgtacc gattatttta
    27421 atgtattcat cgatattttt tatcaacatt ttttttatga ccaactggtt ccgacggttc
    27481 catttgccac ttacttccag cacatattta tttaggggca aacgtaagat atattttgcc
    27541 aaatagcgat ctttaagcag gccattgatc acacggtcct caagctgcaa gttgatccca
    27601 gtcagcatca ttggctgttc ttgtaaaacg tacgtatcaa ttaaaagacc ttcaaaagtt
    27661 tccacaagcg tcttcctttc ttttcaaagt aacctgaaaa caggcactaa ttcttccatt
    27721 ttgatattcg ttgaagtgga tgcgatcatt ctttcttttg tccactgtaa tctagtataa
    27781 caaaaaagag cagaagagcc aaaaagaaaa aagagcatcg aattctatag gaagtctcat
    27841 tggcacggcc taaattatta caaaacattg attaaaaaaa gcccctgcat cgcaggagca
    27901 tcccctatat cgttttaata ggtcaatcaa aaagcagtat caaacacatt ttgtatcaaa
    27961 tcaggaaata atagataagc taagcccaga atcaaaagta ccgctagaag aaaccagccg
    28021 attgttccta tgaaatactt taatttttcg aacattgtat cacctcgttg tcagtatatc
    28081 acggatcagt aaattggggg tgttaagaat ctcctaaaat agacaaaatt tgttctattt
    28141 tcctaaagca aatacttgta agatacagta agtgatacca gaagcaatga ccggtccggc
    28201 ggcgattcct cttaagaaga caacacctaa aatcgtgccg aaaacaagtg caacagtgat
    28261 ttgtggatca gaagcaatca atcctacacc cttggaagat aagacagcca ctagcccacc
    28321 gcaaaaaaca gctacccagc caatagggga tttaaaggct tctaatagat cacgtagacc
    28381 gatttctcct gttgcgatag ggattagaat agccgctgtg atgatcgtca ccccaatatt
    28441 gattcctttg ccttgaagcc aagggaaaaa ggaacgggag gctggtatga gcttcataat
    28501 caaaacaaaa gctgccgcta tcatcagact ttgattttta gctaaaattg ctacaagtaa
    28561 aataattcct aaaaaaagcc aactttccat gaaatatatt cctcactttc ttcgaaacaa
    28621 tcataacatg tttttagaaa gttacaaaag taggaattca tccttcaaga cctattcata
    28681 agctgttttt ttcataaaat aaacaaaatc ataaaaaaac actttgctta tttgacagaa
    28741 gacagccgcg ctttgtatag tggacaagac ttacattttt ttgaagaatc agaaagatca
    28801 ggtaaaggtc ttagtaaacg caagccattc agaataacca atattgtgct tccttcgtgt
    28861 cctacaactc caagaggtag atttaaaaat tgcaggaagt tagaagcaat caacaagaga
    28921 ataacaagaa tcgaaaaaat aatattttgt ttgatgatcc gccgcatttt taaagatagc
    28981 cgatgactgt agacaaattt atttaagtcg cttttcatta agacgatgtc tgcgatatcc
    29041 atagcggcat ctgtcccttg ccccatagcg acacctaaag aagcggctgc taaagcaggc
    29101 gcatcattga ctccatcacc aaccatcgca tttacttggt aaagttcatt ctcttttttg
    29161 actagttgtg ttttttcttc cggagtacag cttgcatagt aatgttcaat ccctacttgt
    29221 tcagcaatgg cctttgctgt gccagaatgg tcgccggtta tcatacttgt atggatgttt
    29281 tgggatttaa agtaggaaat tacttgttgg gtatttgctt ttggaatatc tagcagacct
    29341 aaaacagcaa ttaactgctg atctttagaa agataaatca cggttttgcc ttgattttcc
    29401 aaacgttcga tcatttccgc aatctctttg ctgattatca tcgtctcagg atcatatgcg
    29461 tgcttgccaa ctttccattg cgcacctaaa taggtagttt gtaatccaaa accagtaatt
    29521 tcttctattg gtaagtattg gatttcttca ggtattgtgc aatcgaaata ttgcaaaatc
    29581 gcatgagcaa gtggatgcgt cgttttttgt tccatagcaa caagaagttg ttgtgccaaa
    29641 tccttttcat caaagaaaaa gctgtcagtt acaactggtt ttcctttagt gagcgttcct
    29701 gttttatcaa aggcaatcgc ttttaattca gaaagctttt ctaaatggat accgcctttg
    29761 attaaaatcc cattttttgc agaatttgaa agagcagcta gagaagctgg tgtagcagat
    29821 gcgacaagcg cacaaggaga agccacaact aataatacca ttcctcgata aaagctttca
    29881 tcccaactcc aacctaggaa aaagtggggg agaaggatca ttagaggaac agcaagcaaa
    29941 atcaatttta cataattatt ttcgattcgt tcgataaatg aagctgtttg agttggcgta
    30001 ttctgcgctt cttctactaa ttggataatc ttagcaaaga cagtttcgga actcgttttt
    30061 gttactcgaa tcgtgatggg atttccttga ttcagcgttc ctgcatacat ctcatcgcct
    30121 aaatattttt ctacaggcac agattcaccg cttaatgcag actcatcgat cgttgcctgt
    30181 gtgctttcta aaaagccatc tatcggaaca gctgctcctt tagcgacaaa taccaagtta
    30241 tccaccttta gttgagaaac atctacttct tgcatttgtc cattttcttt taataattgg
    30301 gctttttgtg gttgaagatt catcaaagca gtaatttctt tttgactttt attagttgtg
    30361 tattcctcta atgcaccgct taaacaaaag atgaatgtca acattgctcc ttcaaagtaa
    30421 ttaccgatca aacaggcacc aatagcagct aaagccatca ataaatccac attcagtgtc
    30481 cgatcattcc acaattcttt caagccttcc gatgtttgtt tatatccacc gatgataatg
    30541 gcactgatat aaaaaagtgg ggtgatggta agagagaatc ggtcgaagat aaaaccgaag
    30601 atcataaata aaaggcagcc gattgttgct gcaaaagctt ttttctgatt agtcatcgtt
    30661 attagacctc cttgtcaaaa caaggatagc ataataaaaa tgtaattgat aataataaca
    30721 gctaaatgag aacgataatg gttatcattt ggtgattaag cttgatgttg tttttattat
    30781 ttataatgat tattattaaa gaaaataaaa aaccagaaca tttctcaaat aagag
//
LOCUS       DACDSY010000013        30108 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDSY010000013 DACDSY010000000
VERSION     DACDSY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.013"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(405..1349)
                     /locus_tag="IWK44_000553"
     CDS             complement(405..1349)
                     /locus_tag="IWK44_000553"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9549109.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1645..2589)
                     /locus_tag="IWK44_000554"
     CDS             complement(1645..2589)
                     /locus_tag="IWK44_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9549110.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2810..4024
                     /locus_tag="IWK44_000555"
     CDS             2810..4024
                     /locus_tag="IWK44_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9549111.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4046..6061
                     /locus_tag="IWK44_000556"
     CDS             4046..6061
                     /locus_tag="IWK44_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP9549112.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6144..7517)
                     /locus_tag="IWK44_000557"
     CDS             complement(6144..7517)
                     /locus_tag="IWK44_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9549113.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7543..7827)
                     /locus_tag="IWK44_000558"
     CDS             complement(7543..7827)
                     /locus_tag="IWK44_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9549114.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7829..9844)
                     /locus_tag="IWK44_000559"
     CDS             complement(7829..9844)
                     /locus_tag="IWK44_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9549115.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10023..10328)
                     /locus_tag="IWK44_000560"
     CDS             complement(10023..10328)
                     /locus_tag="IWK44_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP9549116.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10417..11448)
                     /locus_tag="IWK44_000561"
     CDS             complement(10417..11448)
                     /locus_tag="IWK44_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9549117.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11655..12530
                     /locus_tag="IWK44_000562"
     CDS             11655..12530
                     /locus_tag="IWK44_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9549118.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12682..13524)
                     /locus_tag="IWK44_000563"
     CDS             complement(12682..13524)
                     /locus_tag="IWK44_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9549119.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13616..15493)
                     /locus_tag="IWK44_000564"
     CDS             complement(13616..15493)
                     /locus_tag="IWK44_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9549120.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15490..16911)
                     /locus_tag="IWK44_000565"
     CDS             complement(15490..16911)
                     /locus_tag="IWK44_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9549121.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16914..20303)
                     /locus_tag="IWK44_000566"
     CDS             complement(16914..20303)
                     /locus_tag="IWK44_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9549122.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20560..21951
                     /locus_tag="IWK44_000567"
     CDS             20560..21951
                     /locus_tag="IWK44_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9549123.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22024..22671)
                     /locus_tag="IWK44_000568"
     CDS             complement(22024..22671)
                     /locus_tag="IWK44_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9549124.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22679..23365)
                     /locus_tag="IWK44_000569"
     CDS             complement(22679..23365)
                     /locus_tag="IWK44_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9549125.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23371..24444)
                     /locus_tag="IWK44_000570"
     CDS             complement(23371..24444)
                     /locus_tag="IWK44_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9549126.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24666..25340
                     /locus_tag="IWK44_000571"
     CDS             24666..25340
                     /locus_tag="IWK44_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9549127.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25345..26685
                     /locus_tag="IWK44_000572"
     CDS             25345..26685
                     /locus_tag="IWK44_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9549128.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26834..27820)
                     /locus_tag="IWK44_000573"
     CDS             complement(26834..27820)
                     /locus_tag="IWK44_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP9549129.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28544..29575
                     /locus_tag="IWK44_000574"
     CDS             28544..29575
                     /locus_tag="IWK44_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9549130.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29684..>30108
                     /locus_tag="IWK44_000575"
     CDS             29684..>30108
                     /locus_tag="IWK44_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9549131.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT         8927 a         5635 c         5405 g        10141 t
ORIGIN      
        1 cgaacggttt tttatcaaaa atcgtcattt ttccatcaaa atctgcttat gcaaaattat
       61 aaaaaaattt tgcagttttt cttacttttt ttgtcgttta attgaatgat aaaaaccagt
      121 ttctttattc agttaatcaa atatgaacga attagatatg aatcattgta tttgccgttg
      181 cggaaggatt ttttaattaa ccacagataa acattgataa cgattcgctt attgtaatca
      241 acagattaaa tttcatatgt tcatatgtac ggatacattt tcggaccgat taattttaac
      301 cattctgttt atttgcttac ctattagata tcaataaaaa actgcttaac agctatttca
      361 gctgttaagc agttttagga tttttagttc atattcattg tttcttataa tacagtgttc
      421 aatacttctc ttaaagcact tgttgatttg ctgaacattg ctttttcttc ttcgttgatt
      481 gataattcaa caacttcacg aacaccattt tcgtcaacga ttgatggcac acctgtaaag
      541 atatcttttt cgtcgtattc accagttaaa taagcagata caggcaatac agcttgttcg
      601 ttattcaaga tagcttttac gatacgtgca gtactcatac caataccata gtaagttgct
      661 tttttacgat caatgatctc ataagcagca ttacggactt tatcagcaat cacatctaat
      721 tcatcttggg caatacgatg gtctttttct acaatttcaa acactggttt accaccgaca
      781 gttgtatgtg accatgcagc aacttctgaa tcaccatgtt cacctaagat atagccgtgg
      841 acgctgcgtg ggtcaacttt taatttcaat gcgatttctt tacggaaacg agttgtatcc
      901 aatgttgttc cagtaccgat aacacgtgat gttggcagac cagattcgtt ccaagcgata
      961 tatgtcaaaa tatctactgg gtttgaagca acaacgataa taccgtcgaa tcctgatccc
     1021 atgatttctt ttacgatttg gcgcatgatt gacgcatttg ttttaactaa atctaaacga
     1081 gattgaccag gtttttggtt gataccagct gtgataacaa cgatatcagc atctttacat
     1141 tcttcatagc cacctgacca tacagccaca ttttcatcgc cccaagccat accatctaaa
     1201 aggtccaatg cttccccttc tgctttttct tgattcacat cgatcaatac taattcatta
     1261 gagattcctt ggttgatcat tgcataagcg atacttgttc caacaaaacc tgtaccgacg
     1321 attactactt tacgacttgt ttttttcatg aataaacagc tccttttcat aactatttgt
     1381 tactttcatg aaaagtatag tcttgtttat tcattttatc aacatgttaa ttataatagt
     1441 tgttattata atttttgatg gaaacgaatt cctgaatacg cataacagat aaatgacaaa
     1501 tgtttacacc tatatcccct gataatacaa ccaaaagttt agaactgaaa ataaatccaa
     1561 aaaaattcgc tgcaacgata taataagaca gcaatcgcac ctaaaacaat tttccttcat
     1621 atgctttagt gaaagatttt cttcttaatg tttatttctc cattcattgg gagtacatgt
     1681 tgaatacttc ttgaataatt tgaataaaga agtcgtttgt ttatatccga tacgattagc
     1741 gatttcttcc aacgaaaaac tcgtttcttg aatcagttcc aacgcaaagt cgaatttttt
     1801 ctttagaacc agttcttgaa aggaagcatc aaaatacatt ttcaatttat tactaagata
     1861 gaccggattg tagccaaaat gagctgctgt ttcttttaac gtacaagaag taaaatgctt
     1921 atcgatataa tctaatactc taagcatctc attttctgcg catccatctg ctgatgaaaa
     1981 gctttgttgt tctaataaat gagtcaattc gatcagtaat agtgatgtat agagatttaa
     2041 cgaacggatc ttatgactgt atgtatcaaa ataaatcaac agcatatttt caatcaaatg
     2101 cgtcaaacga cttgtttttc cgccgcgaaa atgaatgaat tgtgtatgtt cccctttttc
     2161 ttttgatgca tttatcaaaa aagaagaaag tagattattt gtgcttgcca aatgattcaa
     2221 tatctctgta gataaggttt cttcttttaa taagatgttg attaaaatat cctcttgccc
     2281 tgtgtattcg attctttgag tagcttcttt atcaatgatc accacgtcac tttcttccat
     2341 ctctatcctt tctccattaa taatttgtaa acatgtaccg ctatacatat aattgatttc
     2401 aacaaaatca tgtgtatgct cgggaacttc cgcatatcga cggtgtttac tgatatgaat
     2461 atttccctgt tgaaatagat cccctcggtg gatttttggc ggtgtctgaa gatggtaagc
     2521 taagtttaaa tcattgatat tcattccttg ttcacgctgc agagtttcga ttgattcttc
     2581 gttacgcaaa aaaaatgtct aattcagcta gttccaattg actccctcct ttttgtaagc
     2641 ggtttctttc gcttattagt tcacgctgta ctgctattat atgcaaagct atttttggtt
     2701 tcataaagac tttctatggt tgggacatga acttcctgtg attccgctat tgagagaata
     2761 accacttcat actatatttt tgataagcgc ttacaaaaat gaaaggaaca tgcttatgaa
     2821 taaaaagatg atgaaatttt ctctgctgtc agtctctctt attttgactt ctgctggtgc
     2881 agtcacgtct gcaattccca gtatgtcagc tacttatgcc acgcgttctt tatctgctgt
     2941 tgaattatta acgactgctc cctcttttat ggtcactatt tttgttttga ttagtagttt
     3001 tattgctagg aaaatcggac aaaaacaaac tgtgatcatc ggattagtga ttgcatcgat
     3061 ttgtggtatc ctccccctct tctcacaaag ttacagtatt gtcttagctt caagagctgg
     3121 actaggtatt ggctttggat tgatcaattc tttggcagtc agcatgattg ctgcctttta
     3181 tcacggagat gaacgagcct ctatgattgg ttttcaaagt gcatttcaag gatttggtgc
     3241 agctttaatg acatttgttg caggacagct agtacagttc ggttggcaat ataccttttt
     3301 agtctacgcc atcagcttac ctattcttgt cttgtttgtt ttgtttgttc ctacacctga
     3361 aacaactgaa cttcaatcgc atggcacagc cccaacaaga caagtaatca aaacgaatcc
     3421 taaaatgatt agttatggta tttttctctt gctggtcatc gtggtatata atgctgtatc
     3481 gattaaatta gccacagtcc tgacgactta ttctctagga aacgaaacta atgcagcgaa
     3541 tatcttaaca atcatgcaat tggcaagtat ggctgccgga gtttgttttg gctggcttca
     3601 gacaaaagca catcgctata tgcttacact ttctttacta ctgatgggaa tcggctttgt
     3661 attgattgcc actgtgataa acctgtatgt gattgggttt ggtgcgatcc taacaggaat
     3721 ttctttttcg ttattcattc cttacttatt caaccaagta agtatccaag cgcccaagta
     3781 cgcggagagt ttcaatactt caatcttatt ggttggcgct aatttaggta ctttcattgc
     3841 accatatggt ttgctctttc tctcttttgc ttctcttggg agtaaaacac ttgctgtttt
     3901 cgtcaatggc agtatcttgc tgttgattag cgcgcttatt agccttctag tagtacacag
     3961 gacgaataga aagacagtga taagagtaga tgaagaaata aaagaacaat tgacaacaaa
     4021 ataaatttga tggaggtcgt gtaaaatgac aaatgaaaaa cataaagata gatggctttg
     4081 gtatcctgga gactttgaga tccgccatgg actgctacaa aatttccaac gggaagaacg
     4141 aggttttgac tggccagcgt actggtacat ggatgattgc catcggaacg tcaagtttaa
     4201 gagatattac tttcttgatc aaccttccat gtttaaagtg acgatccaag gagttgggta
     4261 tgtagaaatt aatggacaaa aacatccatg tggaaaatgg ctgacttgtc cagctggtaa
     4321 agcaaaaatt cgtatctttg taggacatac atcagggcta ccagcgatgt ttatcgaggg
     4381 agacgaagta aaatcagata ttggctggac agcaagtaat tttattgaag aatatccagc
     4441 tggctggagt ccgctttatg tcgatatcgc aaaagatcca aatcaaatct attatcaaaa
     4501 agaatgcgtc catccagtca ctgaaacgga gattggcgga ggaatcttat tcgattttgg
     4561 gcgagcagtg aatggctgtg tagaactaat atctctggat gaccatatcg aatctatcac
     4621 tctatgttat ggagagtccg atgtagaagc tatggatatc gattattgct actataagca
     4681 aataggaatg acaagaaaaa tgaaagctcg taaacgcgct tttcgctatt tgtttatacc
     4741 agaggtttct actggaatgg ttcaacttca agtttttcat gaatttttat ctctttcgaa
     4801 acaagctgct ttttcaagcg attcttcttt actgaatcag atctggtcag tatcagaaga
     4861 agccttcaag ttatgtagtg atttgttttt tatcgacggt atcaaacgag atcgctggat
     4921 ttggtctgga gatgcgtatc aatcattcat gatcaatcaa tatctttttt ttgacgagga
     4981 aatcaataaa cgaacgatat tagcattaag aggacaaaat gaaatcaagc agcatatgaa
     5041 tactatcgtt gactattcga tcttgtggtt gattggtatc gaaaaccact atatgatgac
     5101 gaaagactat gaatttttac gcatcgtcta ccctaagatg caatcattaa tgagatactt
     5161 gatggagcaa acgaatgaat tagggtttat ttatgggaga gcaaaagatt ggatatttat
     5221 tgattggtct gaaatggata aagaaggaac gcttgctgca gaacaagtct tattactgaa
     5281 agcctatcat tccatgatca cttgcggaga tatcttagct gaaacatatg gagagaacga
     5341 aaattatcat actcatctct atcaagaaaa atatcaacta ttgaaacaaa acctatttga
     5401 ctacttttgg gatgaagaac ttggcgcttt tatcgattgc tatgaatctg ggagaagaaa
     5461 tgtgacgcgt catgcgaata ttttcgctgt tttattcgat tttgctaaca aagaccaaca
     5521 gcaatcgatc ttaaagaatg tcttattgaa tgacactatc acacagatta ctactccata
     5581 tttcaaattc tatgaacaag atgctttatg caaactcgga cagacgaata tcgtctacca
     5641 aacgattctt gattattggg gagggatgtt atctcatgat gcggtaactt tctgggaaga
     5701 atacgaccct actcaaacag gaaatgaaaa gtacagtatg tatggagatc cttatggaaa
     5761 aagcttatgc catgcatggg gagcaagccc aatctatctt ttaggacgct attttatcgg
     5821 tttgtatcca acaaagccag gttatgaaga atttatgata caacctaatc tagattcatt
     5881 tgaaacactg aattgtacgc tacctatcaa agatggaaag atttcgcttg aaaaaacaaa
     5941 agataagatc gtgattactt ctgatagaac aggcggaaca gtgaaaatga atggtaaaag
     6001 ttatcctctt ctagcaaaac agccacttgt cttaacaagc tattcaaaaa taaatgcata
     6061 aaacaaaaaa ctgcttgcga catatcagaa gactttaaat cttcttctga tattgtccaa
     6121 gcaattttta ttttgaagtt tgcttaattc tgttctttag aaacaatgtt tgttcttttt
     6181 ccggcaaaaa gaaagctaga taatacgaca agtaaaaaga aagcgatgat tccaactgtc
     6241 aaggctattt gaccgtattc tcctagtttc ccaagtacaa gtcctggtac tagataatca
     6301 gtatcggcaa atgtagaaga agcgatttta agatctccta atactggaag gataaataat
     6361 ggcagccaac taatcaataa gctattgaaa gcagaaccta ataccgcccc tcttctaccg
     6421 ccagtcgagt tgccgaagac accagaagca gctccgcaga agaaatgtcc tactacccca
     6481 ggaatgataa ctgtagtttg caagcctatc atagcaaaca tggatagtgt tcctacaaag
     6541 aagctaacaa aaaagccaat taatacagca ttaggtgcat atggaaaaac gataggaaca
     6601 tctaatgctg gtttagaatc gggtacgagt tttttagcaa tcccttggaa tgctggaacg
     6661 atttctgcca acaccattcg aaccccttga aggacgataa caaacccagc tgtgaatgtt
     6721 ccagcttgga tcagtgcaaa gattacagcg ttgtctccat tgcttagttc tctttcaaca
     6781 aatccaggac cagctaacaa agcaagtgcg acatatacga tcgacatcaa gagcatgatc
     6841 gatacagtag aatctcgcaa aaagctgaaa cttttaggaa tctttatgtc ctctgtagaa
     6901 cggtctttgt ttccaaaaaa ctttccgatc catccactag aagcataacc gatatttccg
     6961 gtatgcccta acgcaacttg attgtttcca gtgatgttct tcataaacgg ttgacaaaga
     7021 gcaggcgtaa tagttaatag tgttccttcg aagaccccgc ctaataagat catccacaaa
     7081 tttgcagtaa accccgcgct tactaaaatg actgctgtta gacaagaaac ataaagcatt
     7141 gcttgacctg taagaaaaat gaatttatat cttgtgaatc tagcaagtaa aatattcacg
     7201 atcatcccta ctagcatgat caaagcagta ggtgtaccgt attgttgcaa agcaatcgcc
     7261 actactgctt cattgctagg aactactcct tgagtatgca gagctgtttg gaacatcgaa
     7321 gcaaaagggg ttagtgaatt actgattaaa ccagcaccgc cgatcagtac taggaatccg
     7381 acaaatgttt tgatagttcc ttggatcact tctgaaaatt tcttcttctg aagaacgaga
     7441 ccaagcagtg caatcaaaga aacgaggatg gagggctgac tgacgattga cacaaacaaa
     7501 ttcaacaatt tatccattga tagttcctcc cttgaatcat ttttattctt ttgacaagat
     7561 cttcgttact ttttctttta cttcttcgct gtcaatgatc gattgcaaag gtacgagctt
     7621 ttcatcaggt atagaagtta gcgtatcttt caaagaatgc tcaatgaaga aataatcagc
     7681 catatcgcta gatacacttc caacatccgt atgacttact tcaaactcgt ccacatctgc
     7741 tccaagatct gcaagtgctt gtttgatgtt catctctacc ataaaacttg ttcctaatcc
     7801 actttgacat actgcaaaaa ttttcatatc attcgtctcc ttttcttatg atcgctaata
     7861 tttcttcttt tgttgatgcg tgtaataaat cttgaagatt ttgtttatta gaaagtagct
     7921 ttgttaaatt tgccaaagcc cgaagatgtg cctcattatc gatagcagct aaacagatga
     7981 acaaactaat gggatgggct ggattatcaa gcagatccac aggttgttga agtttcagca
     8041 aagacatccc tacttcgttt acgccatctt ctggacgagc atgaggtaat gcgatgtgat
     8101 cgccaatatg gataaacgca ccatactgct ccacgcgctc gatcatagct ttgatataac
     8161 tttcttctat tttattttct gataatagcg gctgtgcggc aaatgtgatt gcttcttgcc
     8221 agttttttgg ttgatccgat aattggatca tttcttctgt taataattca gatagcatag
     8281 gacgttgatc ctccttcttt tctaaaagtt tgttcatttt acgatttaat acttgataga
     8341 gcttttcttc gtctacgcct tcttttaatt cgatataagg cagtagcgcc gacaacaatt
     8401 cttgggcaga aggcattgaa aatcctggta tcagccaatc tctttgtact tgattgatca
     8461 gctgattttt ttccaagtca gtcatgattg gatgcagaac atagagagac tttttcttat
     8521 cagtaagtgt gagaggtaca gtagaaaaaa taatatcgta agaagtttcg tcaacctcgt
     8581 gtaagcggtc agctgaatta gtctctgtaa aaaagatcat cgggaataat tgctgaagtt
     8641 cactttttaa gatcaatgaa gagctaatgc cacttggaca gacgatcacg gctcttattt
     8701 ttctatttct ttcttctgcg tccgcttttc tgatctctcc gccaaatagt atagtgaaaa
     8761 aaccaatctc ttcttcaggg atgctcttcc ctattcgtct ttctaacgga agtaatgctt
     8821 ttcttgtcat ttcgtagatg gaagcatatt gatgcttaat ttgatcgatc atcacattag
     8881 gtaaataaaa agaataggaa atacgaaaat aggcagggac caagtgataa aagacattca
     8941 tcaataattc acgaaacgaa gcaaattcta cggccatcag gaattccatg cgatggatga
     9001 cttctgaaga acattccagc aaaaaatcaa gtgcaggctc ctggacatct ccttcaacga
     9061 tcgatgctaa gcaaagagag aaaaatataa tctccgaatt ttgatgttcg gaatagccaa
     9121 ggccagtcaa tacttgtcga ctaatgtcag tgactgcaag agaagccaac agttcagcat
     9181 cttttaccag ttccatggat gtttttccaa ttcgaatggt tgtcaaactc aaataataag
     9241 ctaattcatc cattcgattt ggtgcgaatc gaagatctga tagtgaaatc gttttatcta
     9301 gcaaatctct aactttggcg taataatcaa tggaaagggt tgcggttaaa tacgtgagcc
     9361 caaatcttcc agcaagtgac tgcatcaaac gagcagtgcg attttttgct tctcttctta
     9421 atattgcttc tgaccctagc agcctaaaac cagttttcct agtatacgac agctgacaat
     9481 cttccacttc taattcattt cttatctttt tgatatctga caggactgtc cctttagaaa
     9541 cctgaagaaa atcttgaaaa tgaaaaacag aaacagacgt tgcagtcgaa taaaccatca
     9601 aataaagaag atctcttcgc tcttgttcgg tatacacagc ttcaaatcga gtaatatcca
     9661 aaaatttttc tgcccagtcg attgctcgga atttctttat acggatacgg gtatcatcca
     9721 catattcggc aaaaggagtt aattgtttga tcgcctcgct tgtatttact tcgatgatct
     9781 ctgctgcttt ttctagactg atttctcttg gtgttttcag gagataacta attttgtagt
     9841 tcacagcgct gttcacctcc tacttatatt ctagtatggt agcgctttta aatcgagggg
     9901 tgtttggtac aacttacagt tggatttttt ttacgactgt accgaacgat ttctatcgaa
     9961 tggatacaga aaaaaagcag gtgaccaaat gtctcctgct tttatgaatt gatttcgtcc
    10021 gatcaatgat gatcatgttt attttttgag gtcagttctt caaatttttc tttgacatct
    10081 tcaaccgctt tttcgatatc tttttcaaag tgattgggct cattaggctt tgtttcctct
    10141 tctacagaat ttgaaccaga taaagctgtt tctttatttt ctgtttcacc agagtttgtg
    10201 ccagctaatg attcttcttt gtcttctgtt tcaccagaat tagttcctgc taaagccact
    10261 tcttcttctg ttttaggggc cattgaacct cttaatcgtt tgtgtttttc accgtcataa
    10321 tttgtcatgt tggttcctcc tagtagttct atactttttc atcgtacaat aagacagttt
    10381 atgcgtcaaa aaacaaaccc gattcttaca aatatgtcat atttttcctt ctgattcttg
    10441 aatgcttttg attccttctt ccaaaatttt catttgtaag attgtttctt catctttgat
    10501 gactttttct gcgccattaa ctaatgtctc gtaaatccct tcgtagacac ggctgtaatc
    10561 accaacctcg gaaattactt tttcttcatg ataactccct tgttcatcta tataggtcaa
    10621 tacaccataa tgttcgggca aatcgatacc aaagtcttca tgatcaggca tgtaaaacat
    10681 tttcagatgt tcttcttgtc gatcttttgt ttgtttcacg aacatccctt tttttccgta
    10741 aacgacgaaa cttggacgtt ctttgatgcg gaaataactg gattttatcg aaattttcat
    10801 atttccatag aaaaaatcca gatcgaagta atcattcatt cgtcctttcc ctaaaagttg
    10861 acgtacatca tagctgatat catctggttc gccgaaataa gaaagaactt gatctactgt
    10921 atgacaagca tgtccatata aatagctgtt cgcagtagaa aattcatgcg tattttctgg
    10981 tacttccggt ctgaaataat cataatgcat ttctacttct aaaatatccc ctaattttcc
    11041 gctttcgatt actttttgga ctgtcaagaa gtcagaatcg aaccgtctgt tttggtaaca
    11101 ttggataaat agaccttttt cttttgctaa tgcaaataac tcttctgctt cctttgacgt
    11161 ttctgcaaaa ggtttctcta ctagtacatt cttaccatgt tcgagtgcca tttttccata
    11221 ttcataatga aattgactag gtgtagtgat gacaactaat tggatctcag gatcatccca
    11281 aagatcagac agctgatcag tatattcgat gtcttcgaat gttttccaca ctggttcaga
    11341 tggggaaaaa atttttttga tattgatttt gttcgtaagt tttcttgaaa aaggcaaatg
    11401 atagcgattt gtactctttc cattgccaag ataagcgatt gtaagcatga gtggtgcctc
    11461 cttttattga tgttttcagt atatcctaga aaagcgcaga aagtatttat aatagctatt
    11521 ctggtaaaaa agttcagaaa aacacttgtt atcattcggt ttggtgaaat taatgttcaa
    11581 agtaaggata aaaatcaaaa agatagtagc ggaaccaagt gtttatcagt aagatagaat
    11641 ttggaggtca gatcatgctt attgaagaaa aactaacaaa acaagagttg tttaccacga
    11701 cagagaaacg gattgtcgat tacatccgta ggaatatcga agctgcagtc tacatgacga
    11761 ttgaagaatt agcaaaagct acgtatactt cccattcagc tatcattcgt ttatgcaaaa
    11821 aaatgggatt cagcggattt aaagaattcc gtttcgagct ggggcgggaa gttcatcaat
    11881 tgattgctca gttcaatcaa acgattgatc ctaattttcc tttttcaagt gaagatagca
    11941 ctcagcaaat tgcaaagaaa atggcggatt tatcgatcca gtcaataaaa aaagcacaat
    12001 tgcagattga acatgaagat ttagatcaaa tcggtaaagt attgaacaag gcacagcgaa
    12061 tctttctttt tgcaaaaggt gattctcaaa tcacggcaag gaaattccag aataaaatgg
    12121 tcaagttgaa taagtttttg atcatggctg aagaatacag tgattcttct tggaatgcag
    12181 ccaatttgac tagcgaggat tgcgccattt ttattagcta cagcggtcgc atccatcatt
    12241 atgaacgaat aatgacctat ttgaagcatg tagaggcccc taccttgctg attaccggaa
    12301 atcagcattc agaaatggcc aaacaggctg atatgtgtct tgttatatcg caggaggaat
    12361 atgattttgc taaagtagcg actttctcct ctcaaattgc cttcgattac gtattaaata
    12421 cgttattctc agtgatatat tctcaaaatt ttgaagagaa tatccgcaac ttgaaaaaca
    12481 aacaagaatt gatgcaaaaa ggccttctta aagaatatcg gaaaacttaa gaaaagatcc
    12541 tcatttagta ataagaaaga tagtgtttga taggctctct ttgatatcat cataaaaaca
    12601 ggctgaagtt ttctttttca aacctcagcc tgtttgttta cggaacgatt cgaatagata
    12661 tattatagaa atgaggaata gctaacttct gtttcttatt ttgaacgatt gacgaaaaag
    12721 atatataaga aggaggaaaa tcagtataca tgcagctaac aatccataca ggaaaaatcg
    12781 tttgttctcg gatactttct gtacttcttt ttgaacttct tttgcatgat aaggaacccg
    12841 gtgccctctg acaagtaagc gatgactgtt gaccatataa ggtgtacaag ttaggagtgt
    12901 cacgaaatcc tgcccttttt cgattctcag atcttttgta tcagtcggtt ctaccgtctt
    12961 gatctgatcg acttgataag cgagtgtccg tccattgatt tcgatataga aagaatcacc
    13021 taaagtcagt ttcggtaagt ctgtgaacaa ctttgcttga ggcagtcctc gatggctgga
    13081 aataacggca tgtgtatttt ctccccccgt aggataagac gttccttcca ataatgaggc
    13141 acctttttct agcagcacag tattcgtttc atcaaaaatt ggcaaatgga cattgatctc
    13201 agggatcgtc aaaataccga tcgtatgttt ctcaaaatag gttctatcct ttttcgcagg
    13261 ttcttttttc ttagtaaagg gatctgcacc tggatttcca ccttcttttg ctaattgctg
    13321 gtttttcttt gtcatgtttt cttggatttt agccatgact tcttcatttt ctttgaccgc
    13381 ttgttgctga taatgagaga tgatctgctg atccagataa ttattcagcg cgtcgctaac
    13441 aaatggataa gctagtatcc ctattcctga gagaagcaag aaaagcatca gcagatctag
    13501 aagtttcctt ttctttggtt tcatttgtgt tctccttgtg atgactgttt gataataaaa
    13561 gaaaaaggag catttgcgta atccgctgct cctttttcca attcttgcaa aaagactaaa
    13621 tctgactgtg cttgcgtcta gcaaagtaca gtccagcaat cagtagaagg actactcctg
    13681 caccgatata cacatagatt cccttaccgc ctgttgaagg aagtgtacct ttgtgtttat
    13741 ttggtatttt ttcaggagaa atcaactgtc ctgctgttac ataagattgt tcatcgatag
    13801 taaatgctac acggtttgtt aatggaacat atttttctgg cgctttcgtt tcttccagat
    13861 agtacgtgcc atattttaga cctgtcacat cgattaaacc gttacttgtg gttgtaaaaa
    13921 ccgttgctga ttctttcgcc gatacccagc tgacggcttt tgtggatggg tcgatcgata
    13981 aatatttcgc tgtgtcacta tcttgatcac gaacgacgaa ttctgctcca gcaagtgttt
    14041 ggtctgatgt aacgtcacca tctactttaa caaatcgttt gcccccagta acgacatcga
    14101 ctgacggtgg tgtttgatca tttgtatgcc cgttatcgac atttgcttgg ttagaaaatc
    14161 ctttggttgg atctgctttt tcgttcaaat gcatatagta aacgaatttc aatgtaccgc
    14221 caggagttaa tgaaggaata taattcggat caactgaaac cgtgaatccg tctgtttgct
    14281 cagtgacaga ataatttact gggtcgattt ctttatttcc atcatataag gcataagcat
    14341 acgttcctga agaatcatta tcaaatgtta aagcagcgtc atgagtatcg ataagtttga
    14401 atgttgtgta cttgttttgc gttccttctt tatcagcgat acctaatggg atattgacag
    14461 aaatctcata ttgaattttt tcaccgattg cgacatcttt tccattcaat tgaggtgttg
    14521 ttttatcaac tttagacgta tcatttttga tggtcttttc tactggtgtt tggctatttg
    14581 ccaggatctc aaaaggcgtg attgtttgct tttcaatcat ttccgcatta tcaggagctt
    14641 ttacttcttc taaattatat tttccaactt ctaaatgatt aacgatcaac tggccttttt
    14701 caatagatgc ctcggcaaag tcattgttgc cgatgtcata agaatgacca gtaatgaaat
    14761 gtttggcttt ggtttgatca gttgtccaag tgtacaatcc atctgtgaca ctttggatgt
    14821 attttttgac gcttggtgtt ccttcttctt tagaaataat aaattctgct ccatttagtg
    14881 cttcgttttc ggcagtaccg atttttgtaa ctttcaacgt tccatcatta ccgactgtat
    14941 ttttagggta gagatggata gtatctagtt cttctgtccc gtatttataa gagccatctg
    15001 cttgtttgat catctcatat acagggaaag ctaaaaccat gtttgcggca gctgacactc
    15061 cgtcttttgg ttcttctttg atcgtgtaga ctgcatcttt cccattttgt tttttaggta
    15121 aagataaagt gacatttcca tcagcatctg tcgttcctga agcaactggt gtaccaggag
    15181 tcaaagactg gactgcttgt tttgctgcat ccacggacgc tcctttatct cgttgcgcat
    15241 aaaattcttg agtgacgtta taaactgaaa atgaaatatc ggctaatcct tggtattgat
    15301 cgaattcgct catttctttc ccgctgtttt ggattaaagg atcgggtaaa tcagtcattt
    15361 tctttttatg aaggatgact tgagctgctg tctcctctgc aaacactttt tttgcgccga
    15421 agctgtttgt gagtaatggt aaaataagga aaaggactcc taacataacg tttatttttt
    15481 tatgattttt cattttttca ctcctgttct ttttttgata aacaaccaga ttgcaaagct
    15541 tgcgaatacc ataccaacaa cgcctaaaag tgtatttttc gtttcgtttg tttttggaag
    15601 aaatggacgg ctattttctt ttttattgac tactttcaat atactgtccg cctctttatc
    15661 ctcagaaaga aaagttgaaa tggtaaacgg aatttctgtt tcacttaaaa tataaccaga
    15721 aggtgcttcg atctctttta atcgataact tcctgttttc aacccggaaa ttgaaaaagc
    15781 gccattttta tcggaaatca ggtgaagcgc atctgattcg ttctttgtcc acttgtatcc
    15841 gttggctgtt tgctggaggt aatttcctgc tttgttttta ataaggaata ctgcgccagc
    15901 taatcgttta tctggtttat ttgtctcgat tttgacaaat tgcttactcc ctgtatatac
    15961 agagactttc tttgtactga caccaaaacc aaaatccagt gttccgttgt tgttgatttc
    16021 cttgttcgcc tgtgctgtac tgcttagctg catctgataa gaaatggtca gtttttttcc
    16081 agcatatttt tccaacgaag ggatcgaaaa taaaacatca aatccgtgag gtttcttgtg
    16141 tatgacaaag ccttctgtca gcttttctcc attcgatgaa atcgttaaac tattaggtaa
    16201 aaacgttaat gcttcatccg cactatctga caattcgaac ttttgatagt caaggatatt
    16261 tacaggaatt gtcgtatgca aggaataagt gattttttca ccaatcgtaa aatcatttct
    16321 aggctcttct aataccttct caaaaggtgg atcagggtag tcgttttctt catttttagg
    16381 atacaaatgg atagttgaaa gtttttgatt gttttgtcca tgtacaggaa gaacaactac
    16441 catattctct gccttttctt tgacgacttc tggtactttg gattcaatga aaagatacgc
    16501 tttatctctt ttttcttgat ctttagcggc cagagaaaaa gaagcgatac catcttcgtt
    16561 gttaagagtt gtagttgttg cttctgcggt aaacataccg gaagacgcac cgttttttgc
    16621 gatctctgct tgtgcttctt ctaccgtttt gcccttttcc cgtagatggt aaaaagaatc
    16681 tgtgacatca taaacttgga atgtgacacc atttaatcct cgatacgttt gtaataaagc
    16741 tttttcttgt ccgtcatttg gatgattttt cggcagttgc ccgtttggga aaagcaattt
    16801 gtgtaaagta atctgaacca tttctgtttc tgtttctgtt gctacctgag taaaagctgg
    16861 tttaaatagt aacaagaaga gacacatact aagccaacca agttttttca tatttagctg
    16921 ccccctcttt cttttttcaa aaaaagatac caaatagaaa acgcacaacc aatcatccct
    16981 actagataga ttcctaaacg gccaattcct cccgtttcag gtaatggtac ttttgcctga
    17041 ttcgtgatgt ctaaagaaat ctggttgttt ttggctcctg gtgacagaac agattcatga
    17101 tcttgtccat ccacttgaat tgacccatct tcgtgtataa ctatagtaac tggctctttt
    17161 agaccttggt atccttctgg tgtaaaagtt tcggtcagcg tataagttcc aggttttaaa
    17221 ttttcgaata gaaaggtatc tgtttcttgt ccatcttttg gcgattctaa gtccatttct
    17281 ggtccctgca gtctgaattt tgctcctttt aatgtctgtc cgttgtcttc ttttttgtgt
    17341 accgttagat caaacgcttt tagtcgattt tgatgagtga gcgtccatac attatttgtc
    17401 atctcggtat tttttcccgt gtaaatgatt tttccatatt tatcgatttg gaattcataa
    17461 tttcccggcg aagtatcata tccggcagga ccagctgttt caacgactcg atatttacca
    17521 ggtgtgagtg aatcaaaact ggcaagacct ttttcattcg ttgtttggct gtcaattggc
    17581 tgataagtac catttgcttc ttttttctgt agggaaaatg ctgctccttt aagatttatc
    17641 tctttgtccg tcccttgcat cgcatactta cgtactgcta caggaacttc aacaaaagga
    17701 ttttcaatag tcaactggat cgtatcatcg gaagtagtga ctgtttttcc atcaatggtt
    17761 accgtaccat cagaagcgat cttgatttcc caagtagtct ttttgcttaa tccatgccct
    17821 tctggagctt ttgtttctgt cagcgtatag gtcatttctt tttgcaattt gacattttct
    17881 ggaagagaat aggttccgtc gccatgatct gttagtaaag tatcaataga atctcctgtt
    17941 aatttgaaaa cagcgccaat aagatccttt tcacctgtag aggaattttt cgttattttc
    18001 aagtttaacg gtgtgaattg tttagtattt ttccatgtca ttgcatctat ttgttgagaa
    18061 tcgtatcctg gtacaggtaa ttctttgatt gtttggtaac tgaatgcttg accttgatta
    18121 ttgtattgag gtagtgataa tatctcttga taactttctc cgctatttgc agataatttg
    18181 tcaatgtctg cacgttccca cgtatttgtt gtatctttag ctggtttaat gattcgaata
    18241 tatccgtttt tccaagctgc agcatttgtc gtatgttccc gttgaatctc aaaagtaact
    18301 tgatctggac gatcagctag attgttgtca aattcttccc ataacttttt gatgtgaaga
    18361 ctgactccgg gagctttagc agatggtata ccgaattcag ctaattcatt ggtatcaatg
    18421 tttggctgga aagttgtcct gccgttcatt tgataccaga aatttggatg gaagtcctca
    18481 ttttctgttt ggattctcac ttggtaatgg atttggattt cttggtcttt tcctaaatag
    18541 atctgattgc tcttgatggt atttccttct atggaaatag atggagtgac cgttgtagga
    18601 cttgtcccca cactcttgac tgaaagtgta ccaggctgat aaatgaatgg ttctgcgatt
    18661 ggatcattta tttgtccatt gcttacagtt gctgagatct gtacagcttt tttggctagg
    18721 tattcagaga tatctgttgc atgatcagct gattcatagt aaagatcgcc tttttcatct
    18781 gatgaaacca tttgtcgcat acgagactct acttctgctt ttgagagacc agctgccgga
    18841 tcgctttgaa gttggatgcc aagaccatgt atttcgattc ctcgttcttt gagtgccatc
    18901 gcttctccga tggttgcgat aaacgtactg tcgattcttc tggatagatt gttttggtca
    18961 ggtgcatcat agattcttga gattagagaa gtatttcccg gccgatcttg cgtattagaa
    19021 aactgagttc cgtaataatt gctgcttgat tgtgcgtgta cccgctgtac tttataggaa
    19081 aatgttggta caccatccgt cagcaaaacg atcacttttt tatgtccatt tggaacggat
    19141 agcatgcttc ctgcatctct tagtgctttt tgtgtaaaag taccaccatt tgtccgtgaa
    19201 ggtgtaatgg attttacttg atttttcact gaatcaaatg aacccatctg tactgcaccg
    19261 ttactgtagc tatatccttc gcttgagtag ccgacatatc ccatattgat tttgtctgtg
    19321 ataccgctat ctgctaaagt atcgacaaaa cgatcgacac caatctttac ttcaccgatc
    19381 cgattattgt cgttcatact tcctgaccag tctacgacca atacgagatc aagaggcgtg
    19441 atatcttttt gtacatttcc acgagcattc aaataaacat caaacaatcc gggtgtactt
    19501 gtttcctttg catactttcg tatcgcatag tctgcttgat taccggttcc tccatattcg
    19561 atatacgaat gggtccgatc attaggatct ccattccaag aatttatacc atcccattga
    19621 tttgttccgt ttttattgcc ttgatgatca agaacatttg tatttcctgt aggttgccaa
    19681 ctggctttag gataagtgcc ctgatcatct gttgtatatt ctgtaggaat cgtttgatta
    19741 gatggcacac tcacagctgg caaggataat gtaggcaatg taaatgggct gacagaattt
    19801 tcgttttctt ttgatgattc ttcttctatt tcgtctggct ttggtactga cacatctgta
    19861 tctttcttat tttctgtctc atcaataatg tcatattctt tattatcgac gatcagcttg
    19921 tttgcctggc tgtggtaaag tgctggatcg attggaaaac tcagatcgat agtttgttta
    19981 gtgtttgatg agatttccaa cgtaattttc ccgctattta cctgataagc atagccatct
    20041 ccttcttttc tttccacatc acttaagtaa ttttcgacta gaccaatttc tttttctatc
    20101 ttttcagcac tggtgttatt cgcatttagc ttgatgttca gtgctttttt tccagactga
    20161 tccgaaatga cttcagctga aattgtttct aaaactgttg gatcatctgt agtgtctgct
    20221 attgcagata atggcacaaa gtttgataaa ctcaatatta tcagcatgaa aataactttc
    20281 agtttcttcc ctgtggtcgt catttttccc ctcctttttc tttttttctg ttttgacgtg
    20341 aatagtgaaa attattaaaa taaacaggcg ttacataatt ttatttattt taatatgata
    20401 attattatac gaagggcgct aaagggaatc acaccaattt tcataccaat tggtttcatt
    20461 tatgaagttg attccttcga ttaatgagcg tttcattgaa agaaaagggt tgagggagaa
    20521 aaacatatga tcaggttgtt ggaatgaaac tatagaaaga tggaaaaaat cattcgaatt
    20581 ctgtccattt ctgacaagtg gatgactaaa aaagaacttg ccaaacgaat tggtagttca
    20641 gaatctacat ttgtaaggta tatcgaagag atcaaaatcc gctggggaac ggatgtaacg
    20701 attcaatctt cacaaaaatt ggggtatcgt cttgaacatt tgaatgtacc agcttattta
    20761 aatgttctga tggaaatgtc gcaaagttct acaaacagtc gtttattaaa tgagattatt
    20821 gtcaatccgg gtaaaacgat tgagtattat tgtgaaaaaa tttccatcag ccggtcttct
    20881 tttacacgta agttgaaaca atgcaatcaa attttagaaa gctatttatt aaaagtgatc
    20941 gttgaccaag gttttcagtt aactagcctt aaaagtgagc tccagttgcg tatctttgtt
    21001 acctttttct ttttaagtta ctacggaaga cgcacgttgc catttccaat cgaaaaaaag
    21061 aagatcaaag aaattatggt caagaataat tgtcagatga atttgtttca ggaagaaagc
    21121 ccatatgagc aatctttctt tattatgtat tttgttgtgc atctcatgag agaatcccaa
    21181 ggaactccat ttgaatggaa agctggagac tcttatgtac agttgataaa tatcgataaa
    21241 aaagacttct taatcttgaa aaatctctta ggaaaattga agtacagcag ctatataaaa
    21301 gtagtgaact attttgtcaa tacgattttt tcagaatttt cggagaaaga aaaaaatgaa
    21361 atcagtgagc agatacgaaa aaatatgtcc tactgttcgt tttcatccgc tgattgcaaa
    21421 gaaaaaaacg aagaatatgt tgttcaatta ttaacgcaaa tttatttttt ctcccgtatt
    21481 ttcccatacg atacgactca tttgacgatc cgtgcaactt actttgcaca gcagatgcaa
    21541 caccgccaga tacgtctata tgctggattt cgtgcagaac ttcaatttta ttccactcta
    21601 ctgaatcaga atctgattta ttatttatct agctatatct tttggatgct tatcggtaat
    21661 cctgaaattc accattatac ttctgataaa aaaatactga ttatcagcga cttaagtctt
    21721 cgacatagtc aatatattga agaatatatc agtgatattc ttgctgtgca taaaattcac
    21781 tctgagacaa ctgctattac tgaagatcag ctgtcgaaat acaatcttct cgagtatgat
    21841 ctgatcgtta cgaatcaacc cattttgaat gcccatgttc ctcatatatt gattgatgat
    21901 agtgtgtcat tcgcaaatga agaagaactc atccgcttat tcgagttatg aataatcaaa
    21961 aaacagctat gtataaacca ccctgataaa aatttcagga caaatacaca tagctgtttc
    22021 tttttattgt attccttcag caatccaacg agatttatag cgaaagtctt ttaacaactc
    22081 ttcttttgtc caattatttg caatgagatc cgtcaaagtg ttcattacga gggtcatgga
    22141 aaagtatagg aaactaactg cttcttccga cgattgaatg gagaaatgat tgattcttaa
    22201 taattccagc catttttcgt tcattggaga atcagccgat agttctctac gtttggttgt
    22261 ctgctgatca tttcgctggg ttaacaagta taacctttgc caatagatac tttcgtgatc
    22321 taagtcgcag caccactcta aatatttttc gatccccaaa aagaaatgtt gtttgttttc
    22381 gctaatgaat tttaagatat cttgatggac tatgtgagaa ctgcttttga tcgtatacaa
    22441 ataagcatct tccaaatctt gaaaatattt ataaaaagca ccgcgcgaca tccccatctt
    22501 gtcaacgatt tctgagactt tgacttggct gatgtgctgt gagcaaaata tctccagtaa
    22561 taggtcatcg attctctgtt tctttgcgtt cgataaattg aaaaaagttt cttttggcat
    22621 ccacatctct cctttatctc tgattatacg tggatagccc ttcattgtca tgaaggattc
    22681 attccttctt tttctcttct tctgtcaagt acccatcttc cattcgataa acgcgatcac
    22741 tccattgaat catacggaca tcatgagtaa ccataatagt cgctttttgt ttctcatgag
    22801 cttctttaac tagtaatttg acgacttcat atgcatgatc tgtatccaaa ctagctgttg
    22861 gttcgtctgc taagatcaag cttggctcat tatataaagc gcgagcaatt gccactctct
    22921 gtctttctcc accagaaagg tctttaggat aactattttt aagtgactgg atatctaaag
    22981 attgcagcaa ggaatcgatt tttgttgaat cttctttttt tgaagtaact tggtcaacta
    23041 gttcaaattg ttcctttacc gttaaaaaag gaatcaaatt agagctttgg agaataaatc
    23101 ctatttcttt aaagcgtaaa tctgctcgtt ttttttctgg aagctcagtt agtgagtgac
    23161 cgttgatcac tatttctccg gaagtcggtg tttgcaatcc tcctgaaatg gtaaggaaag
    23221 tacttttacc tgaaccagaa ggaccaatga tgctgacgaa ttctccttcc tttacataaa
    23281 aatcaattgc tttcaaagct gagacctttg tatgaccaga accaaatgtt ttggtaactt
    23341 ttttcatcgt taatattttt ttcattttta ttaacctcct attgctgtaa ttgggtctac
    23401 tttggttatc gtacgtatcg aaaacaatcc gcctagcatt gcgacaatga tcaaaatccc
    23461 actatatagc aaccattgtg accaaaaaac agcaaaaggc atcgcgcttg gtaaaatcag
    23521 actagtcaaa taagcaaaca aaagagcaag cagaacgcct accaccccaa caataaagga
    23581 ctgtgctact aaagatttag cgatgaagaa atttttgatc ccttgtgctt tcattactcc
    23641 aaaaatagca gtcttctgaa gtgtcatcac gtacatgaaa ataccgacca cggcagcaac
    23701 cactaagaac aaaaagtata tcatcgcatt taaggtcata ttttgggcag aatagccagg
    23761 aatactttca atgaatgtcc caatagttaa taactgtaga cccttcgatg ctttttcttt
    23821 cgaaatttta gctgaatcct ttattacgat cccattgatc ggctgttctt cttcagaagc
    23881 aaacggctgt tgtccatact tcaaagcagt ccacgtatct agggacgcat agatgacggg
    23941 agaaactgtg taataagttt caggaaagat accgacaatc gtcaaaggat cgtcgtaact
    24001 gccaattttg atcgtatctc caattttata gcctgaatca gccagatttt gagagatgat
    24061 gatttcattc tttgttttaa attcatgccc tttcgtgagt tttggtaata aaaatgcttc
    24121 tttcgttgtg ccgaaaactg taacattttc tttgtttttt cctttgattg ctccgctgta
    24181 caatccgatt ggtgctttct ctcctccttc aatgtatttc agatctcctc ttgttaattg
    24241 tgaagctgca aatacttgat ttgcatcctc cgacaaaaca atctcttgtg cgtcccaatc
    24301 atcgattgct tttttaaact cttctgaaag tccattcgct agtcctgaca acatgaatac
    24361 gacatacgca atcaagaaca taataccgac gatcaagcca tatcgtaatt tagaatagcg
    24421 aatttctttg atagctagaa acatttgttt ttcctcccta tgatagattg atagtgacaa
    24481 aatgtcactt gttgaatagt gtaactctaa agtgacaatt tgtcacttgt gttgtttttt
    24541 tcttatcaat aaattgtcta agacatcttt cgataaaacc ttaacatgag tcataaaaaa
    24601 tttatttaag ccttatttat gctttccatg ataaactaaa gtgaccaaat caatgaggtg
    24661 ggaaaatgat taaactatta gtagtagaag acgataaagt attatccaat aatataaaag
    24721 aaatactcgc tgcaatcggc gagataacac aagtttatga tggtagtgaa ggactgttcg
    24781 agttggaaag tggagtttat gatcttgcaa tcttggattt gatgatgccg gaaatgaatg
    24841 gatatgaagt gttagcaaaa atacggaaag aaaaaataca gactccagta ttgattttga
    24901 ctgcaaaaga tagtttggaa gataagattg aaggatttga acgaggtgca gatgattatc
    24961 taacaaaacc tttctatcga gaagaactga tcatgcgcgt gaaagcatta ttgaaacgct
    25021 cgctaggtct ctttgatgaa aatgttctga agtataagga tatcagttgt gatttaaaaa
    25081 gcaatgaagt cttgtataaa gaacaagttt tgcccataca aggaaaagag tttgaattgc
    25141 tcgtttattt tttacaaaat aagggaatca ttttgacaaa agagcagata tttgaccgga
    25201 tctggggatt tgattctgat acgacgatta cagttattga agtttatatg agcaacttac
    25261 gaaaacatct gcgaagtact ggattggata aagaaatcaa aaccttacgt aatgtcggtt
    25321 atatactgca aagggtgtga tgcaatgaat ggagatatta cacgaaagca acgtagtcgc
    25381 ttttttgcga caaatgtagc agcatttgct tttatctttt ttcttttggg attcattact
    25441 ttacagatat taaaccaatc tgcatacaga gaaacagatc tgtcattaga gaaaataaat
    25501 tttgattctc gaacagtaca attagagata caaagatatc agcaaggcga tcccttttta
    25561 aacccgactc aaacggaaca aaacagaaca aacatggaac agccattaat ggaaccatca
    25621 aatgaccgtt ttaacactca agtgatcttg tggtctaaaa atggtgagat attgaataaa
    25681 gcagctattg gtgggcgttt caaccaaatc aaagaattaa aaatggatac gaaaaatctg
    25741 aataccatcc aaaatattga attaaatgaa gacagtggct catctgccct ctctttccgt
    25801 tcgattacga aaaaagcgga gacgaatcaa gatggcgttg cctacatcca aattttagaa
    25861 aatacaaatc aaatcaagaa ttcattagcg acttttcgca cgattatgat tttgtgtatg
    25921 attgtttttt ggattctctc tattggtatc agttattact tgtctagtct aagcatgcgc
    25981 cctattattc tttcttggag aagacaaaaa gaatttgttg agaatgcttc tcatgaattg
    26041 cgcacaccgt taacgattat tcaaaatagt ttggaacatc tttttaccaa gcctaatcat
    26101 actattattg aagaatccga aagcatcgct caagcattgt cagagacgag gcgcctcact
    26161 ggattgactt cggatttgct gacaatcgct cggaatgatt ctgatcagca gctgctttca
    26221 aagcaaatga tcaatacaca agaatatatc gaacaacttg ttaaaccatt tcaagagatg
    26281 gccatcatag atggaaaaga gtttattctc cataacgatg caacattaga agtaccaatt
    26341 gatgaaaaaa aaatccatca agttttggtt attttgctgg ataatgcatt aaaatatacg
    26401 aatccagaag ataagatcat tttggattca tctgttaacc ataaagaatg gctgattcac
    26461 gtaaagaata ctggcccttc tatcagtgaa gaagatcaag caaggatttt tgatcgattt
    26521 tatcgggaag atcaatctcg ggcgaaagag actggcggat acgggcttgg cttggcaatt
    26581 gcaaaacagg taatcgaaca gcatcatgga cagatttctg tatccaatta tcttccacaa
    26641 ggagtcgatt ttaaagtcca attgccgttg aagcctaaaa aataatgctt atccctaaaa
    26701 aagggctgtg acaaaaatct tgtcacagcc cttttttgtt gcgaataaac ggtgttccag
    26761 aagtagcttc ttcggaaata agccgaaatt tctttaagct gaccacttct gtcacaacct
    26821 ctttagctta cgcttacgat tttttattgc ggtcttcaat gtgtacatca ccgcttgtgt
    26881 caatatccaa ttcttctcgg ctaacatctt ctgtcacttt ttcaacatct tcatgttctt
    26941 ctttatgaat cccaacttct tcacgaataa ctgtatgttt gcttacatct acttgctctt
    27001 ctttgattgg gatagtaatc gtgtcatctt ccaatttttc gtctgttccc tgtgaagatt
    27061 gatctgttac tggtttgcgt tcgatgacga tttcttcacg tttgaccggt acttctaccg
    27121 tttcagtatc gttgaccaca tgtttgtgga tgtcgacttc tccagttgtc acatcatgtg
    27181 tatttacatc taatttttct tcttttagtt gaattgtatc atcatctaga tttggtttgc
    27241 gatcgatgtc ttcagtcttg cttgtaccca ttgtatcagt ctcgccaggt acgtccatcg
    27301 gatcagttcc tggtgtcata ttcaatggat ccgttgtatt ttgaaagcct gtcgtgccac
    27361 ctacgtccat tggatctgtt ccagtgccag tttctaatcc agtccccatt gtgcttgctg
    27421 ggctaacagt atcttgtgta tccattgtat cttctgttgt ttctggctga cgatagcctt
    27481 ttactgtgat gacaaatttt ccatcagaaa tatctcctct atattgatat aaaggatcat
    27541 tttctggtgt agccgtctca gaatcatagg tatcaaaaga aaaagtatct tttatttttt
    27601 cccataaaga acggtcttct tcaccatcca tccgattttc atctgtttca acatctatac
    27661 cagacaatcc gtcaaatcct agtctagatg ctgcttcatc agtagtataa ataacgatat
    27721 catctcgttc atacccttcg ttacgcaacc gttcaattac tgataatgct tcttccctcg
    27781 tactatagct tccgatgacc gttaaatttt tctcttccat gtaaattcct cctagcaaat
    27841 agatttatga ttggtatatt gttacttata aatttattct ataggagacg attgttttct
    27901 acaaatattt agattggaat ttgattaaaa caatttaatt gaaatttgaa aacatttatg
    27961 ttaatgaaag tcatattcta ataaaatgtc atttgtttct atatgaattt ttatgaaaaa
    28021 tttcatataa tttccactcg ttgtgatggt ttaatttatc caatccatca ataagcaaac
    28081 aacttatata aaaaaggatt aattctcaat ataattgctc ctaccataga ataaatcttc
    28141 atgtaaagta atgaagtacc aactatttct aatacttctt taagaattat cccaacaata
    28201 tcgttagaca aaccattatt gaataaatgg agagccaatc gattctgaaa aaatgttttc
    28261 taatttttcg gaaataaaag aaagagcctc ctgccattag aacgaaccaa atccatttca
    28321 ttgctcatga agtaaccaca tagctaaaaa gaggataaat caattaaaag atcaaaagaa
    28381 tgactgcact aataaaataa ctaaaaatgt atcttcttac ttcatatctt ttagctagta
    28441 cttagttaaa aatttgcttc ctaagatcaa gacaaactgt ctatcttcat ttgatgaaat
    28501 gcggtaaaat atagaaaatg aacactattg gagggaattt gtaatggaca gcaaaactag
    28561 aaaacaactg caagaaaagt taaagcaacg tacacacttg ctcccttccg tctttcaaca
    28621 gtttttagaa gaaaatcgtt cttccctttc attaggcagt cgctatgaat acgcaaaaga
    28681 ttttcatctg tttactgatt atttgagaaa agtattttct gaagaagtgg atgatcagct
    28741 gattatccat ttagaaaaac aagattatca gaatttctta gctaaaatct atcgacatac
    28801 tcgttcgttc caaactacag cgaaccaaga aacggagcaa ttgtttgaaa atagctatta
    28861 cgggatttca aggaagcaat attcattaaa tcatttttta cgatttttgt atcaaaaagg
    28921 actgattgaa gaagagattt ctcttttggg tacaagttta ccggaaacaa ccaaagcagc
    28981 tccgcgatat atcgatgcag aacttttcaa agattttgag catttgttta tcccgattga
    29041 aggctttcaa acaagcagag aagctagctt tgaagaaaag aatagaccga ggaaccttgc
    29101 aattactgcc attcttctgt attgcggatt aaagatccat gaagtagtag aacttaaacg
    29161 taaagatgta tctttgacca agcagattct taatctgaat agaaaagaaa acagactgaa
    29221 taaagtacca ataccaaaag aagcgatgcc atttctggag aactatttta agttaacagc
    29281 acaaaatacg gacgaaaatt cttttgtttt tcgttcctca catgatcagc aaatttctcc
    29341 tcggactatt cgccaaattt tgagcaaaat tacagtatat aaaaacgtat cgccagaact
    29401 atgcagaaaa acatatcgtt attatgcaga actatattta gatgatgctg acgcggtaaa
    29461 ttatttatgt ggtaatcgat acctctcatc ccattctttt caagatatct gggaaaagat
    29521 ggttgatttt tcttatcacg aattagcggc attcgttcgg gtttcttcca tataaaacaa
    29581 atgtatgatc tttttttcac aataaactat gttattatat aaaggattat gttacgatta
    29641 tcttgaattt gtatattact taacaaaaga taatggagtg tgaatgatgg agaccataac
    29701 gtattcccct ttgaacttat ttacgaattt tcaagaagct gcagctaact tttccgagac
    29761 cccgattatc tttgatgagc cactttcagc tttcccagaa ttaggacttg aaacaactta
    29821 caaaaaatgc tgcgaaagtg tgttgaaacg ggcttatcaa ttggctcatc ttggagtgaa
    29881 atccggagac aaagttatta tttataagag ttccgctttt gacacatacc ttctagctgt
    29941 ttctgtgtct taccttggtg cagtgcctgt catgacttct tatcatctac caacaacaac
    30001 aatggaagta tttatcgatc gtttagaaga ccctttcatt ttatttgatg atgagacaaa
    30061 aaagcgagtg agagaaattt caaatggaac aaaatcaaaa cagattcc
//
LOCUS       DACDSY010000014        29291 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDSY010000014 DACDSY010000000
VERSION     DACDSY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.014"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(47..895)
                     /locus_tag="IWK44_000576"
     CDS             complement(47..895)
                     /locus_tag="IWK44_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9549132.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1119..3059)
                     /gene="thrS"
                     /locus_tag="IWK44_000577"
     CDS             complement(1119..3059)
                     /gene="thrS"
                     /locus_tag="IWK44_000577"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP9549133.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3396..3575)
                     /locus_tag="IWK44_000578"
     CDS             complement(3396..3575)
                     /locus_tag="IWK44_000578"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP9549134.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3622..5445)
                     /gene="cadA"
                     /locus_tag="IWK44_000579"
     CDS             complement(3622..5445)
                     /gene="cadA"
                     /locus_tag="IWK44_000579"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9549135.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5723..8002
                     /locus_tag="IWK44_000580"
     CDS             5723..8002
                     /locus_tag="IWK44_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9549136.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8095..8742)
                     /locus_tag="IWK44_000581"
     CDS             complement(8095..8742)
                     /locus_tag="IWK44_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549137.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8921..9640)
                     /locus_tag="IWK44_000582"
     CDS             complement(8921..9640)
                     /locus_tag="IWK44_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9549138.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9771..10922)
                     /locus_tag="IWK44_000583"
     CDS             complement(9771..10922)
                     /locus_tag="IWK44_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP9549139.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11139..11879)
                     /locus_tag="IWK44_000584"
     CDS             complement(11139..11879)
                     /locus_tag="IWK44_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9549140.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11883..12206)
                     /gene="rsfS"
                     /locus_tag="IWK44_000585"
     CDS             complement(11883..12206)
                     /gene="rsfS"
                     /locus_tag="IWK44_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP9549141.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12263..12856)
                     /locus_tag="IWK44_000586"
     CDS             complement(12263..12856)
                     /locus_tag="IWK44_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9549142.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12840..13487)
                     /locus_tag="IWK44_000587"
     CDS             complement(12840..13487)
                     /locus_tag="IWK44_000587"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP9549143.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13500..13811)
                     /gene="yhbY"
                     /locus_tag="IWK44_000588"
     CDS             complement(13500..13811)
                     /gene="yhbY"
                     /locus_tag="IWK44_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP9549144.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13827..14936)
                     /gene="yqeH"
                     /locus_tag="IWK44_000589"
     CDS             complement(13827..14936)
                     /gene="yqeH"
                     /locus_tag="IWK44_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP9549145.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14933..15460)
                     /locus_tag="IWK44_000590"
     CDS             complement(14933..15460)
                     /locus_tag="IWK44_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP9549146.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15640..16425)
                     /locus_tag="IWK44_000591"
     CDS             complement(15640..16425)
                     /locus_tag="IWK44_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP9549147.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16418..17287)
                     /locus_tag="IWK44_000592"
     CDS             complement(16418..17287)
                     /locus_tag="IWK44_000592"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP9549148.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17288..18667)
                     /locus_tag="IWK44_000593"
     CDS             complement(17288..18667)
                     /locus_tag="IWK44_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP9549149.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18675..19094)
                     /gene="fabZ"
                     /locus_tag="IWK44_000594"
     CDS             complement(18675..19094)
                     /gene="fabZ"
                     /locus_tag="IWK44_000594"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9549150.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19119..19595)
                     /gene="accB"
                     /locus_tag="IWK44_000595"
     CDS             complement(19119..19595)
                     /gene="accB"
                     /locus_tag="IWK44_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP9549151.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19598..20833)
                     /gene="fabF"
                     /locus_tag="IWK44_000596"
     CDS             complement(19598..20833)
                     /gene="fabF"
                     /locus_tag="IWK44_000596"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP9549152.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20852..21589)
                     /gene="fabG"
                     /locus_tag="IWK44_000597"
     CDS             complement(20852..21589)
                     /gene="fabG"
                     /locus_tag="IWK44_000597"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP9549153.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21601..22518)
                     /gene="fabD"
                     /locus_tag="IWK44_000598"
     CDS             complement(21601..22518)
                     /gene="fabD"
                     /locus_tag="IWK44_000598"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9549154.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22625..22849)
                     /locus_tag="IWK44_000599"
     CDS             complement(22625..22849)
                     /locus_tag="IWK44_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9549155.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22902..23864)
                     /locus_tag="IWK44_000600"
     CDS             complement(22902..23864)
                     /locus_tag="IWK44_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP9549156.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23874..24314)
                     /locus_tag="IWK44_000601"
     CDS             complement(23874..24314)
                     /locus_tag="IWK44_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9549157.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24620..25585
                     /locus_tag="IWK44_000602"
     CDS             24620..25585
                     /locus_tag="IWK44_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9549158.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25642..25872)
                     /locus_tag="IWK44_000603"
     CDS             complement(25642..25872)
                     /locus_tag="IWK44_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP9549159.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25958..26845)
                     /locus_tag="IWK44_000604"
     CDS             complement(25958..26845)
                     /locus_tag="IWK44_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP9549160.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26934..28157)
                     /locus_tag="IWK44_000605"
     CDS             complement(26934..28157)
                     /locus_tag="IWK44_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9549161.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28174..29253)
                     /locus_tag="IWK44_000606"
     CDS             complement(28174..29253)
                     /locus_tag="IWK44_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9549162.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
BASE COUNT         8115 a         6417 c         5049 g         9710 t
ORIGIN      
        1 tacgttttgt tattctaaaa aacttatata aaaaatagta aggtttttat ttcgaatcca
       61 taaaatattc ttggattgct tctcgtgttt ttttcacttt ttcgctaagc ttttcgtttt
      121 tagccatata gaaataaaaa atgatatcga tcgtagcaaa ttgggccaaa agtgaattag
      181 ttgccgcact tcgattcgtg atttcttttg gtctagatgt ttgtacgagt acatcggcta
      241 tcttgctaag cgggttattt ccaaattgcg tcaacgcaat gatttcgata ttcatgcttt
      301 tcgccagctc ggctaatgcg actacatctg cacttctacc tgagtttgaa acaagccaaa
      361 gcactttctt tttctcattg ctgacaagtt gcgggagcaa gacgtggata tctttttcaa
      421 aaatgattgg cttaccgaca cgagaccatt tttgcagaat atcttctact actaaagaag
      481 aggcgccaac cccatataaa aaaacagtct ctgccgattc caaacactct acgacttttt
      541 ggatcgtttc ttcttctaga atatctactg tttcttttat cgctacttgt gcattgttac
      601 caagtttgtt tttgatagag taaaaggact catttgcttc tatatcacta aaaccttcat
      661 cgattccagt ctgtagttct gttgaaatcg tgattttgaa gtccgtcaga ctttgaaaac
      721 cgattttttt ggaaaaacgt acaactgtcg gcgcactcaa cccggaagct tctgctaatt
      781 cattagctgt cattgtaggg accttctcaa tattaccgat aatgtagttg gccaactttt
      841 tttctgctga agaataatct tccatcatac tttcaatacg actaattacg ctcataagtt
      901 cctccaccct tttaatgata gtctatgtaa gtataccata tcagaccctt attcacggtc
      961 gtatactgga taaaaaacaa aaaaacaatc cgcccaacag aatatcttat cccatcaatc
     1021 ccacagattc tattgagcat ctgtgtcttt gaggaatacc tgttatctat tgttcggatt
     1081 gctttctatc tactactttt ttacaggaaa tttacctatt agttttctct gctgtaattt
     1141 ttgacttctg cttccatagc atcaataaag atattcaaat cttctacagc tgtttcttta
     1201 cttccataac gacgtacgtt cactgttgca tcttccaatt ccttgtctcc aacaactaat
     1261 tggtaaggga tcttttgtgt ttgagaggca cgtatcttat agcccatttt ttcattacga
     1321 tcatcaactt ctacacgcaa ccccttcatt tgcaaacgtt ctttgatttc ataagcataa
     1381 tcgccatgtg catcaacaga aacagggatg attgttgctt gtataggtgc caaccatgtt
     1441 ggaaaagcgc ctttgtatac ttctgttaaa taagcgacaa aacgttccat tgtagaaaca
     1501 attccgcggt ggataactac tggacgatgc gtattttccc cgtcttctcc aacatatgtc
     1561 aaatcaaagc gctctggcaa taagaagtct aattgtatag ttgataatgt ttcttctgta
     1621 ccaagagctg ttttgacttg aacatctagt tttggtccgt aaaaggctgc ctcgccttct
     1681 gcttcaaagt aatctagttc taattcatcc atagcggctt tcagcattgc ttgggctttt
     1741 tcccacattg cgtcatcatc aaaatattta tctgtattgt ttggatcgcg atagctcaag
     1801 cggaaacgat aatctgtaat attgaagtca gcatatactg ccaccatcaa ttcaagcgta
     1861 cgtttaaatt catctttgat ttgatcagga cgaacaaatg tatgaccatc attcaacgtc
     1921 atttctcgta cacgttgtaa acctgacaaa gcacctgatt tttcatagcg atgcatcatt
     1981 cctaattcag cgatacgaat tggtaattca cgataagaat ggatcgtgtt tttgtagacc
     2041 atcatgtgat gagggcagtt cattgggcga agaacaagca tttcgccatc tcccatatcc
     2101 attggcggga acatgtcttc ttgataatga tcccagtgtc cagatgtttt gtacaactca
     2161 acatctccca ttattggtgt atatacatgt tggtaaccta gactaacttc tttgtctacg
     2221 atataacgtt cgatcgtgcg tcggattgtt gccccttttg gaagccagaa tggcaaacca
     2281 gaacctactt ctggggaaac catgaacaag tcaagttctt ttcctagttt acgatggtct
     2341 ctttcttttg cttcttcacg gagacggata tattctttca aagctttctt gtcaaaaaag
     2401 gctgtcccgt atacacgttg catcatttga ttgtttgagt ttccacgcca ataagcacct
     2461 gccactgata ataatttaaa tacttgtata cggccagtgg atggaacatg tggtccacga
     2521 cacaaatcga tgaattcgcc ttgttgataa gcagtaatca cttcttcttc tggcaattca
     2581 ctaatcagtt ctactttgta cggatcactt gcaaaaattt ctaatgcttc ctgttttgaa
     2641 agaacacggc gttcgatcgg cagattttct ttgacgattt tcatcatttc tgcttcgatt
     2701 gccggcaaat cttctgcagt tacttggttt tgtccattat ctgtatcgta gtaaaaccct
     2761 gaatcgatag ctggtccaac gccaaagtgg atatttggat agaggcgacg catggcttgg
     2821 gccatcaaat gagctgctga atgacggact aagccaagtg catcttcatg gtctggtgtg
     2881 atgatctcaa tacttgcatc ttcctcgatt ggacgagtca aatcaacgag ttttccgttc
     2941 actttaccag ctaaagcttt ttttgctaag ctttttgaaa tgctttcagc aattgccaat
     3001 gttgttgttc cagactcaaa ttctttgact gcaccatctg gaaaagtaat ttttatcata
     3061 atagtttcct ccttgaaatg ttttgattga tcagatcatc aagctgaaaa acaaaaaaat
     3121 cctagcatct cttgaaaaga gatactagga cgaaaagttc gtggttccac ctaatgttca
     3181 aacgcaaatg cgttcctcaa gcgttgtaac ggtccgaccg cccttgcttc aacaagggtt
     3241 tcgaaagtgg tcatcctttg gttttctcct ggaaaagttt cagcctagct tttcctctct
     3301 tatcgaagac tctcaaattc agttggcttt ctcatcaatc aattatgtgt ttatttaacc
     3361 actaataaat agaaaaatca agtgggaaac taaacttatt ttttccattc cccatttaca
     3421 ccgtatgttc cttttttcat ctcattgatg atgacatgga tgtgttcttt tggtgcctgc
     3481 gtgtttttat gaacagcttc cgtgatatct ttcatcatat tttctaattg ttccggactg
     3541 cgaccttcta ctaattcgac atgtacgaat ggcataatgc tctctcctct ttctgatcgt
     3601 tcttttttca actcgattct atcatactat gtttgtcttg ggtactgaat caggaaaaat
     3661 ggctggatct ttttttgctc tcaaggcact caaaatagag accgtatcca cgacttcttg
     3721 caaaacagct ccgaataatg cggggattag tcccgtactt gcaattaaca tcaaaaaaat
     3781 acaaataaag atgccaatca gtacagactg tttcgctatt ctcatcgtat ctcttgaaat
     3841 cactactgct tttgctacac gcaagagatc atctttcaag atgaccgcat ctgcactttc
     3901 actagcagct gtagaaccat gagcccccat tgcaataccg atatctgcag cagcaagtgc
     3961 tggtgcgtca ttcacgccgt ctcctaccat cgcaacaggt ctttgatctt tagggatact
     4021 ttctaaaatg gtcagtttat cttgaggcaa gctacgggca tgtacagaat ctaattggac
     4081 tttttctgca atcgtttgag caatggcaga tccatccccg gtgatcatca tcaaattttt
     4141 cacatcaagt tttcttagct ccttgattgt ctttgctgct tctgggcgaa tctgatccga
     4201 aaataggatc gttcctgcaa aagatcgatc aattgttaca tagattgctg tattggacgt
     4261 ttctatttct tcagctgaaa caaaagaggc acgtccaatc tttacttcat gattattgac
     4321 tactgcttgg attccttgac cggtcacttc tttcagttga ctaacaggat acagttggct
     4381 gtttttcacg ttgttcacta acgaacgagc taagatatgg ctagattcct gttctgcact
     4441 agctgcatac agaagtagct cttctttttt tattccattg actggctgaa tcgcctctac
     4501 ttccaatatc cctttggtaa gcgttcctgt tttatcaaaa gcaatcgttt ttgtacgtgc
     4561 gagcttttca atcgttgttc cagtttttac aacgatcccg tttttacttg aacggctcat
     4621 tcctgcaact aaagcaatgg gcgcagctag aatcagtgga caaggtgatg ccactaccag
     4681 tacctcagca aatcgtaaag ggtcttttga gaaccaccaa gaaataccac cgatcaaata
     4741 agcaatcaac gtgaaaggga ctgcataacg atcagctaaa cggacaaatc gggcaggctt
     4801 ttcttttgat tcttcgacta gtttaataat catttgatac tggctgtctg cggcacgttt
     4861 ttcagcgatc atttggatcg atccgtctcc atttaaagac cctgacataa tctgatctcc
     4921 aggtttcttg gtttccgggc gggactcacc tgtcaaagaa gattcatcga ccgaagattc
     4981 tcctttggtc actcgaccat ctgcgggtac aagttcgcca ggcttgacta gtaactggtc
     5041 atcaatattt atttcatcga ttactatatc ctgcaatttt ccttctttga atcgatgggc
     5101 aattcttggg gtgttatcta acaaagactg cagttcttta cccgctcgtt tattcgcgta
     5161 atcctctaga ctatctccgc ccgtaagcat gatcaatatc atcaaactag cccagtattc
     5221 tttgacggct aatgttgcaa ggattgcggt aatcgctaat acatctacgc catatcttcc
     5281 tgaacgtaac gtcttgatca tttccaaaaa catcaagaag gcgagaatcc ctccagtaaa
     5341 tgcgacaatc gcaaatgcaa gggccggctg gtgaaaaaca aattcactta ataaagctat
     5401 cccaccaata agcaaagtaa gtaaaaattt tttgaaatgt gtcattgttt tttctcctct
     5461 tttatgtgac ttagagaggt tagtaataaa tttccagctg cctctttcat cttaagcata
     5521 gtccttgact agcacttttt aaaacaatag gccttaattg aaaacaacta tcactatcag
     5581 cgtttccaaa attcatcagt tctacaaaca aaaatattca gcaaggatta ctttttcgat
     5641 tcatcagtta aaaagtacct tttttcgaaa aactatgcta tacttttaat tcgttattta
     5701 cggcggaagg agcaaacatg cgatgaatga gaaacaacta gaacaaacgc acgtggatga
     5761 aacgatcgaa ctaatccaga tcgaacaaaa tatgctggat acaaaacaac aagaattgac
     5821 tcaggaaatg aatcattcga caaaagacac agctaatcac aagattcgag ctggttccaa
     5881 tgaagctttt tatgaatctg ctgttgaata tcgccaacat gagcaggaat tgcttttgaa
     5941 ataccatact cttgaagcac agcaaaaacg cctgcaaacg ttgtctgtca tgaaagatag
     6001 tccttatttt gcccgtatcg actttaaaga agaccgtgaa acagaaacat tatatctagg
     6061 tatcgcttct ttaagagatc tggcagaaga aacaatcgtt attgactggc gcgcaccaat
     6121 tgccaatctt tattatgaag gtgagattgg tcccgcattt tatgaaactg aaacagatag
     6181 aatagatgtc gatctgcagt tgaaacgaca atttaaaatc caagaaggac gtattttatc
     6241 tatggtagat acgtcggaag taatcaatga tgactttttg ctagagatat tggatgaatc
     6301 atcaagcaac catatgaaaa acatcgtctc tacgatacaa aaagcacaaa atgcaatcat
     6361 tcgggacaca acaagtaaag taatgctgat tgaaggaatc gccggaagtg gaaagacatc
     6421 cgctttgctt caacgtattg cttttctgtt gtatcataat cgcaagtggc tcgaggctga
     6481 aaacgttctt ctattttcac ccaaccattt gttttctgat tacatctcga ctgtattgcc
     6541 ttcattaggt gaaagcggcg tgcctacaca gacattcaaa aattatttgg tgcagctgct
     6601 gccagagttt tctttactag atgaacagca acaagaaagt ggttttttgc taggggaaaa
     6661 agatccgata caagtgatga aaagcggatt gaccttagtc gatcagattg atcgctatat
     6721 ccaaggcatc acaaactacg gtcccttgtt ccgtgatatg aaaataaatg gccgtacgat
     6781 cctatcaaag gaatcgattc gccaatggta taaagaaaca aatgaacaat tacctttaca
     6841 tcatagactt tctctattac aaacgaagct cctgaaaaaa ttaggcggtc tgcaaaaaga
     6901 cgaaacacgt cagcaatggg tcaaagatct ggcggaagaa cagctccaag aactatacgc
     6961 aacagatcca aatcttgaat atacagaaca aaaggagcgt tcattacgta aaaaactggc
     7021 caaccaaatc gtacgtaaaa gattccgtcg aatccatcga gggatcatga attatcaatt
     7081 tgtcaatata acgaaacaat atctccactt tctacaaact gtgccacttt ctcttctaac
     7141 acgacataaa atcagcgaga ccaggtggca ggaacatttg acaactgttc gtacacactt
     7201 gcgtcaaaga gaattgcgtc aagaggatgc tgtactattc tttttactga tgcgccgaac
     7261 atatccagtg acggttacac acaaagctcg ctatatcttt attgatgaaa tgcaggattt
     7321 cgcaccatca caagtggcat tgctgcaagc cttgttccca aaagcaaatt tgactttctg
     7381 tggagatcta aatcaaaatg tctttggaaa tgaaacgatc gttggatcat tggaagacct
     7441 tttcgtggac cgtgaagtca ctcgtttcca attaacgaca agctatcgct ccacaaaaga
     7501 aatcactgat tttgctaatc attttattgt gaaagaacat caagtagaaa caacagcaag
     7561 aaaaggagac cttcctttgc tcatccaagg aacaacagat gatatcaaat tgaattggtt
     7621 agcagatacc attcgaacga ctcagggaaa agcccggtat tggcgtacag caatcatagc
     7681 taaaacacaa gaagaatgcg aagctcttta tgctcaactg ccagacgagt tgaaacaaca
     7741 agtccagctg attttaaatg agagtgactt tatgaagcgt tccattattt tacttccagc
     7801 ctttttagca aaaggtctgg agtttgaccg agttttcttg tgggatgtag atgcagaaca
     7861 tttttcttct cagcaagaca aattgatcct ttacaccatg tgtacacgtg cgatgcatga
     7921 attgaccctt tttacctcca ataatgaaag tcctttgtta aacaatatgg atatttcatc
     7981 ttatgagaaa aaagtactgt aagtaaaaag acaactgcta tcattaatgt tagcagttgt
     8041 ctttttattt acaataataa ttgcagaaaa agtaattcaa tcatttaagg tcatttattc
     8101 tatatataaa tccaaatctg tttcaggata ttttaatttg acaagcgaaa taatcatttt
     8161 cagttcttcc atgttttctg gatattctgg gtcaaatacc ttgctatgac ggccaatccc
     8221 gccattgtcg atcaatcggt aaattcgatt ccattcaatg aattggtcaa ttgattggaa
     8281 attgctgatt ggatagccgc cacaatccaa aatctctgat acacgaaatg ctaggtatgc
     8341 actatcgatc actttatttt ttttcgtgtc tatcttctca tttctttcga taagaatttt
     8401 ttcaggacga tgtttcaagc tccactctaa tagtagaaag aaagcctcca tccgatactc
     8461 ttcttcaaat aaccgaaagg ctgaatcaga acgacgaaaa agcagcggaa tcagataatt
     8521 ttgtctataa atcagcattg gtgcgcggtc caccatacac tctaaataac ggttcagata
     8581 ccctgtaccg acatagtttc ctaatcgata gccttcaacg ccgaatcttg cgtatttttg
     8641 cgctaatttt gaacaatcag ttacgttcac agctaactct tctactttgc ggtaaccgct
     8701 taagatacgt tcaaaaggtt ctaaagtgat ttttgcttcc atattcttcc ccgtccatct
     8761 tccacatact tcatatcatt tcacatgata cttatgctct catcatacca caggttgaat
     8821 tagaaaagaa aataaatcac acaaaaaagc aagacaatct gttagtgaga ttgtcttgct
     8881 tttcgaaaaa gactgaatac ttttcgttaa aactggttgc ttagtcttct aatgccacat
     8941 tatggaacac atgctgtaca tcttctagat cttccagtgc atcaatcatt ttttcaaatg
     9001 tttcttgatc ttctggagat agtgttacct cgttttgagg gatcatttgg atctcagcga
     9061 ttgaaaaatc agtgattcct ttttctttta atgcttcttg tatactatgg aaatccgcta
     9121 cttcaccgta aacgatgatt tgtccttctt cttccattac atcgcgaaca tcgatgtctt
     9181 tttccattag gtattcaagg atctcgtcag cgtcttctcc tgcaaaagca aagattgctg
     9241 tattgtcaaa catgtaagaa accgctccgg aaacacccat gttcccgcca tttttcccaa
     9301 atgctgcccg tacgtctgct gctgttcgat tcacattgtt cgtcaatgta tcaacaatca
     9361 ccattgagcc gcttggtcca aatccttcat agcgcaattc agagtatgtt tcatctcctg
     9421 caccttttgc tttttcgatt gctcggtcaa taatatgttt tggtacatta taagttttag
     9481 cgcgatcaat gacgaaacgt agtttttggt ttgcatgcgg atcaggatca cctgattttg
     9541 ctgccgcgta gatttcaatc ccaaattttg cataaatccg gctattatta gcatctttag
     9601 cagttttctt tgctacgata tttgcccatt tacgtcccat attttcactt cgctttctga
     9661 tttgatagtt tcataacgca aaaacgtcct gttccattat acttgtgaat cacgattctg
     9721 tgaagctttt tttctttctt ccttcattct tctcattttt agtcaattat ctatactcgg
     9781 attgggattt gcccgaaatt ctgttccgga atattgcctc ccaattgata gatttgatca
     9841 cttctaacta acagttccgc ttcttgtgcc tctttttttc cgattcggga ataaatagaa
     9901 atctcttttt tttcttttaa gaatcttctt ccagcatcgg taaatccaag tagtcgaaga
     9961 taatttttat tttgttggac aggaatctct tgctttttga tattcagcaa gacataacaa
    10021 gataatcgtt ggatccgtgt ccatgtatat ctttttgttt ttacctttga cacaaaatcc
    10081 tcaaaactat cagcagttgc agctgctgtt ttcatgcgcg attctattcc ttcggtcatc
    10141 tgatagatat ctgtcagttc cagcaaagta gaagataaca accgataacg caaatacggc
    10201 caataagatt cccaagtctg cagcgaacaa gttgaaagat cttcttctgt ctgaggcggc
    10261 acaagtccag ctattctttt tccttcttct aacgcatggc gaatggctgt tgcactggct
    10321 atcctctgat ctgtgatgat tttttcatga tagcctgcac ttttacgtcg gatcggatgt
    10381 aattccatag ggtggacata cttggcattt tcctttgcat aacttaatcc caagatatga
    10441 tttggcgaag aaaaatcaag cgtaatatcg ggatacagac tgcgaaaaac ctcagtcatt
    10501 ttttgcggat aactatacgt ttgatcagac agttcttgaa atgcctcttc tatcctatct
    10561 tgattctctt ggacaaagcg tccgaaactg ctgtaatcaa acgaagtctc ttcttctgtt
    10621 ccaaaacaca aactgtcaca gtccagtgtt tgcaagattt ttactgcacc tttagcaaaa
    10681 tagtcagctg actgaaccgc ccattcgacg ggaagctcca ctactaaatc cacaccattt
    10741 tgtaaagctt cattcgcgcg atgccattta tcgatgatag ccggttcgcc tctttgcaaa
    10801 aaattcccgc tcattacagc aatgacgacc tctgctcctg ttatttttcg tgccatttcc
    10861 acatgatagc gatgtccatt gtgaaaagga ttgtactcta cgataatccc gcagcttttc
    10921 atgcgcatcc tccttcatag ttatcgataa aaaagaaata gagacaagta aaaaaaggca
    10981 aaaatcggaa aaaatacccg aactatataa actattctcg caaacacaat acttctgcga
    11041 cgaaaagctg cactgcaaga gcaatgccta tatcgttcgg atatataatt tatgttgaag
    11101 atttattctt ttcttgttaa ctcgagcagg caaaaccgct agtctttgta tgctacaaag
    11161 aaccagcgct ttgatttctc tgatgctgct tcgtctgtaa aatcagaaaa tgcttcgact
    11221 ttcgaaaagc cgctgttttc cagcattctt aaataacttt ccaatggata ggtacgctct
    11281 tgatgtagtt catcttcacg aataaatcga ccagaccctt cttgttcttc aacgaaaaag
    11341 gtaagaaaat gttcgatgct gtgtttctcg cttcctggat aactatccca caaaaaagca
    11401 aaattatccc cttgataatg atagctatat tctggaaaaa cctcatctac ttgataggtg
    11461 gagtgtacgt caaataaaaa tcggccattt tcttctaacg cttgatagac ttcatcaaac
    11521 acttgctgta cctcttgtct atttttcata tagcacagcg aatcagaaaa acaggtaatt
    11581 gcctgatatt gtccgatttc tgataaatcg agcatattgc cttccacaaa ttggatcgat
    11641 gcgtcttctt catatgctcg ctggctggca atcatcaaca tctcttctga aaggtctaac
    11701 cctgtcactt tatatccatc ttttgcaaag gcaaccgcta atgctccagt accgcatgcc
    11761 agttccaaaa tattttttgt gtcttcttgt aaatgacgtt tcgaaaaagc taaccattgc
    11821 tgataaagtg tttcgtccat cacttcatcg taaatgaaag caaatgtttc ataagccata
    11881 tgttaatcca accattcact aagatcgact agcggagcat ctgaccatag tttttccaga
    11941 ttatagaaat ttcgttctgg cgcatggaaa acatgtacga tcacatctcc aagatcgatc
    12001 aatacccatt ttccgccttc ttttccttca atacgtttta cttcatattt gttttcttct
    12061 tctttatcaa tgatttcttc tgtaattgcg ttgatttgac gttcgctatt tgctgaacag
    12121 atcatgaaat aatctgccaa caaggaaaca tttcgaacgt ctaaagcgat aatgtcttct
    12181 gcccgttttg aatctgctgc ttcaatcgca atttttaatt gttctttact atctatgatg
    12241 atttcctcct aatatgagca gcttattttt ttgctaccca gtaattatat gtttcgattg
    12301 tttttggata aatctgttgt tcttgcgcaa gcaaatgggc caatgtatgt tttgtttcaa
    12361 atgctacagc ttcgtctaga tcagcccatg caatggctcg tgcatcttgt accccaggaa
    12421 aatctcgacc tggttcaata tagtctgcta cataaatgat tttgtcaagg agactcatct
    12481 tagcagcccc agtcgtatgc acccggattg catgaagaat ctctgcatcg gtgattccta
    12541 attcccgctc tacaaacgaa gcgccaacaa gaccatgcca gattgcatta ttataattca
    12601 ataactccgg atcaaaaccg tctcttacga ttaccatttt gaactcctca tctggtcgtt
    12661 cttttgcata atcatgtgtc aatgcagcaa tactagcttt ttcaggagaa gcaccgtatt
    12721 ttttggcaag agccacggct gtttcttcta ctccaagcac atgcttgaag cgttgttcgc
    12781 tcattctcat ctgtattttt tgcatcaggt tttctctaga ataagcagta tattcattac
    12841 tatagtccat attcatacaa cccttcattt tgaatataat caattacttt atctggaaca
    12901 aggtagcgga tagaacagcc ttctttgatc ttttgacgta ttttcgtgga actgatgtcg
    12961 atctcaggta catctaccca aattacagga tatggagtat ccgttgtgta tcctggtcga
    13021 cgtataccca caaagttgac catagaagtc agctcatcga ttttatacca ttttggcaaa
    13081 tattctacca tatccccacc gataataaag taataatctg tatccggatt gttttgcgtc
    13141 aattccttca tagtatcata ggtgtaactt ttcccaccac gagctaattc aatcgtttca
    13201 atctgcaaaa aaggattgtc ttccaccgct aattccaaca tgttgagtcg atgttttgca
    13261 tcaatcgtct gtttctcatc aacatgaggt ggctgataag aaggcatcaa aaatacgcgg
    13321 tcaagaccta aattccggcc agcttgttca gccatcacta aatgtgcaag gtgaacggga
    13381 ttgaatgttc cgcccaagat ccctacttgc tttttcttct ctaagaattg aggcatttct
    13441 tcaggaaata attgtgaacg aacaaatgtc ttagcttgcg tattcatcat ggtcactcct
    13501 tatattgctt ttacttcttt tgatagcctt tgatactttt cttttgtgga aggtttgtat
    13561 aatacaataa cacgtccaat gatttggacg atttcgcaat ctatttcttt ttctaatact
    13621 tgagctgctt cttctgcaat ctcatctgta ttttgaagta acgatacttt gatcagttct
    13681 cttttttcta aagcttcttc aatttgaacg atcattgctg aattgatccc accttttccg
    13741 atttgaaaaa tcggctgaag atgatgcgct ttgctacgta aaaaacgctt ttgttttcct
    13801 cttaaactca taattttcct tcttccttaa atcaatgctt tgcgtttcaa tacatctaca
    13861 ccctttggcg cccagcccgc aattactact ggcgctgtta cagtgatcca gcctagaccg
    13921 gcaaaaacga tgtctgtttt ttctttcaca gaaaactcaa aacgaacaag ctccggaaat
    13981 gcctcgactt catcaggacg aggaggctgt aataatccac ctacatgttt ttgataaaat
    14041 tcgtctgctt tttccagttt cgttcgatga atatttacat catttgacac ataagcaata
    14101 aatgaagcac gattgccgct gataaaatca aatcttgcaa gtccgcccaa gaaaagtgtt
    14161 tgcccttcat tcagctgata aactttaggt ttgatttctt ttaacggtgc aatgatccgc
    14221 aaatcttttt tacctaagta gtgcgccatt tgatggcgat ggataattcc cggtgtatca
    14281 atcaaaaaat gcccgtcatc taaaggaatc tcgattttat ccaaggtcgt tcctggaaac
    14341 tgcgaagttg tgattacatc ttgtactccc gctgtatttt tgatgatttg attgataagc
    14401 gtcgattttc cgacatttgt tactccgacg atatagacat cccgtccatc gcggtattct
    14461 tcgatttttt ctaatagttc cgtcatctca tgagatttct tagcactagt taataataca
    14521 tctactggac gaattcctgc ttcgtaagca cgttccttca tccattgtgt taatttagtt
    14581 tttttcaatg atttaggtaa tatatccact ttgttcccaa ctaaaagtac tggattgtca
    14641 ccgacaaaac ggtgtagtcc tgggattagt gaaccattga agtcaaaaat atccaccaca
    14701 ttgacgatca atgcatcttt ttgccctaat tcattcagta agcgcaaaaa gtcgtcatcc
    14761 gtcaatgaga catcttgggt ctcattataa tggcgtaatc tgaaacaacg ctgacaatag
    14821 acttccccgg aagctaaacc tttttccaaa gccgtttgcg gcgtgtatcc taattcaccg
    14881 ggtttgtttg tttgtataac ggcaccacag ccgatacatt gtaattcttc tgtcattcga
    14941 ttccacctcg ccagcccata tcagggtgtt ttgctaatag ataggccata attctttttt
    15001 cccaaaaacg attaaattgt gtcttccaac tatctgttgc gataatgggt tttacaagga
    15061 tgctacgtat ccctgcacga ttggctccac gtatatcagt cataatctga tcgcctacca
    15121 ttactatctc atcatctgct aaatccaatt gtttctttgc tatgttgatc cctcttgcta
    15181 aaggtttcat tgctcgtgca acatacaaaa gatcaaattt ttcaatcgct cgcgcgacac
    15241 gttttgaatt attgtttgaa acaacaacaa cagggattcc tgcatttttc atttctaaaa
    15301 tccaagtaag caactcttct gttccatctg gattattcca tgcaatcaac gtattgtcta
    15361 aatcggtcaa tactgctttg atgcctagat tctttaattg ggcaggtgta atctgataga
    15421 tggcttctac catccatgtt ggtttgtaat ttgaaaacat gctgtctcct catctgcaaa
    15481 aaatgaagga gcacataaag tactcctcaa ccccgtatta tactgcatat cagcaaaaaa
    15541 aactacctct atactcgttc gtctttcaga aaaagcattt ctttacaaaa tgaccaaaaa
    15601 taaagattga tcacagaaaa atttagaaac aaaaaacaac taatattggc ggaacctctt
    15661 gtatcgtttt tccagtaatt tatgctgatc taatcgaaca agttccgtca atctttcaat
    15721 caacgctttt ttcataatgt gtgcgacttt atatcgtggc aactgctcgc cgttgaacgt
    15781 ttcaggaata actcgatcaa cgattcctaa ctcgcgtagt tctggcgcag taattttcat
    15841 caactcagct gcttctgcgg cgcgactacc gtctttccac aaaatcgacg caaatccttc
    15901 tggagaaagc acagcataaa tcgtttcttc cagcatccag acttcatccg ctaaacctag
    15961 agcaagggca ccgccacttc caccttcacc aatgatgatg gcgatgatcg gtacctttaa
    16021 atcagacatt tcgagcagat ttctggcaat agcctctcct tcgccgcgct cttctgctcc
    16081 tacaccgcaa tatgcacctg ctgtattgat caaggtaatc accggtctat tgaacttttc
    16141 cgcttgtttc atcagtcgta aagcttttcg atagccttca ggatggggag aaccaaaatt
    16201 tctgcggata ttttcctgca gatcccgtcc tttttgaata ccgatcactg ttaccggttt
    16261 gccatctaat cgagcgattc caccaacaat cgcttcgtca tctgcaaata aacgatcacc
    16321 atggcattcc tcgaaagaat cgaagatctc ttcaaaaaaa tccaagcttg tccatcgttc
    16381 tttagctctt gctaactgaa cgacatcatg tgctgtctta tccacgatct atccatcctt
    16441 tctgtgtatg caattcaaga agcgtatgga ttttctgttt caattccatg cggggaacaa
    16501 tctgatcgac aaaaccatga gataataaga attcagcctt ttggaaatct tctggaagtt
    16561 cctgcttgat cgtctgttcg atgacgcgtc ttccagcaaa accgatcaag ctttggggtt
    16621 ctgctagaat aatatcgcct tccattgcaa aactggcagt tactcctccg gtcgtcgggt
    16681 ccgttaaaac agttagataa aaaagacctt cattgctatg ccgtttgaca gcagcagaga
    16741 tttttgccat ctgcattaga gaaaagattc cttcttgcat ccgtgcgcca cctgaagctg
    16801 tgaacaagac gactggaaga ctttcctttg ttgctcgttc gaataatctg gtgatctttt
    16861 ctccgacaat cgttcccata cttcccatga taaaattagg atccatcact ccaataatga
    16921 agggaatccc ctgaatggtt gctttaccag tgaggactgc ttcatgaagt cctgttttat
    16981 cttgcatttt tttaattttt tccgtatatt cgggaaatgc tagcggatct ttggttacaa
    17041 gatccgtatc ccattcttca aaactttttt catcaataat caaagctaga cgctgccatg
    17101 caccgattcg aaaagcgtat ccgcaatgag ggcatacttt ttcttcaccg atgtctttcg
    17161 tataaaggat atgtttgcaa tttggacact ttgcccacat gttatcagga acagatggcg
    17221 agttagcagg tttgctctct ctattaggat tgatccgaat atatttcttt ttcttaaaca
    17281 gagccatcta tgcgcctcct atctccgttt ctggtgtcca attcggtaaa aattcttctt
    17341 gtaaaaatgc tgtgctgtaa tcacctgcta tgactgcagg atgactaatc aaatccatct
    17401 gaaattctgc gttcgtgatg attccttctg tgataaattc acttaaggct cgctgcatct
    17461 tcattaatgc ttcaaaacgc gtatttccat ggacgattat ttttgcaatc attgaatcat
    17521 aatatggcgg gatcgtatat cccgggtaaa ctgcactgtc tactcgcagt cccatcccac
    17581 ctgctggaag cagtaagttt tggatcttcc ctggagaagg agcaaaatgg aaagcaggat
    17641 tttctgcatt aatccggcat tcgattgcgt gtccttggaa tactacttct tcttgacaca
    17701 cattcaaagg ctcaccggct gctatctcca cctgtttttt tactaaatcg attcctgtga
    17761 ccatttctgt tactggatgt tccacttgga ttcgagtatt catttccatg aaataaaaat
    17821 cgccttcttc atccatcaga aactcgatcg ttccggcatt ttcataattg actgctttag
    17881 ctgctcgaac agcagcgtct cctagtgctt ctctttttgt ttgtgagatc actacagaag
    17941 gagattcttc cagtactttt tgattgttgc gttgtaaaga acaatctcgc tcgcctaaat
    18001 gaatgacatg accaaatcga tcacctaaga tctgaacttc tatatggcga gcaggataaa
    18061 tgattttttc caaatacata tcatcatttc caaatgccgc tgctgcttct tgctgagctg
    18121 aagaaaaatg ctgtgggagc tcttcttttg attgaacttt ccgaatgcct ttcccgccac
    18181 cacctgcagc tgctttcagc atcactggat aaccaattct gtctgctatt tccaatgctt
    18241 cttctactgt agataataca ccagtacttc ctggaatgac tgggacattt gcttcttgca
    18301 tcaattgacg cgcattgatc ttatttccca ttgcatcgat cgttgcactt ttaggaccaa
    18361 taaatgtgat attgcactct tcacacatgg aggcaaatcg gctattttca gataaaaaac
    18421 caaatcctgg atgaattgct tcggcttttg tcacgattgc agcgctcagt acttcctgaa
    18481 cattcagata tgaatcaacg gcttttgccg ggccaatgca gattgcttca tctgctaact
    18541 gtgtatgaag tgcttcggca tctgcttctg aatatatcgc tacggtttgg atccctagtt
    18601 cgcggcatgc acgaatgata cgtacagcga tttcaccgcg gttagctatc aatactttag
    18661 aaaacatatt tcacctatcc aatcataaag gttaattccg cttcggctac ttttttaccg
    18721 tctactttag cgattccttt gcctattccg gctgaagctt tcactttcaa tatctcaact
    18781 tcaaggtata aggtatctcc cggtgtaact ttcttacgga attttgcttt gtccaatccg
    18841 ccgaaataag ccgtctttcc cttgaattgt tcaagagaaa gtaatgcaac tgctcctgct
    18901 tgagccattg cttcaacaat caatacccct ggcataacag gttcttctgg aaaatgtcct
    18961 tgaaaaaagg gctcattgat cgttacattt ttcttagcaa cgatccgttc tccttcaacc
    19021 atttcttcca ctcgatcaat caaaagcatt ggataacgat gtggaatgat ttcttttatt
    19081 tcttgtatgt tcattgtttt ccctctttcc tgctttgctt atttttgctg aatcctaaag
    19141 agtggctgtc catattcgac aactgactcg ttttctacaa ggatttctgt gatcactccg
    19201 tcggcctcac tagttatctc attcatcagt ttcatcgctt caataatgca gataacttct
    19261 cctttggcaa cttgatcacc tacttttttg aaagctggtt tgtcgggcgc tggttgcaga
    19321 taaacgattc ccacgattgg cgaaacaacc tcgattccat caaatactgc cgattctttt
    19381 tctttcatta tttctggagc agttgttgct tgttcaggta tgacattgac tggctccgaa
    19441 actggcaacg tttcgtttgt ttgaaccata ttcatgcctc gacttgtttt atttttattc
    19501 atatacaatt caaattgacc ttcacgcaaa tcgaattccg ttagcgagga ttgatcaaat
    19561 tgactgacta attctctgat ttcattgata tccaaaatta ttcctcccag cgtttcatgc
    19621 acagaaccgc attatgtccg ccaaacccta atgaattact cattgcataa gtaaaacttg
    19681 caggtttgct ttcgtttttg acaactgtca agtcaatctc aggatcttgt ttttctacat
    19741 tgatcgtagg tgggataaat tgatgttcta atgccagcaa agttgccaag gcttcgatcc
    19801 cgccggctgc tcccaataaa tgtccagtca tcgatttcgt gctgctgacg taagtatctc
    19861 tgaaattttc acctaatgcg atttggattg cttttgcttc tgcctggtca ttggctggtg
    19921 tacttgttcc gtgagcattg atatatccga cttgttccgg ctgtacacca gcttcttcca
    19981 tcgcaagctt cattgcttta gatgcaccag aaccatctgg agtaggagct gtcatatggt
    20041 aagcgtcaca gtttgcaccg tatccaacga tttctccata gattttggca ccgcgagctt
    20101 gtgcatgttc taaagattca agaactagaa cgcctgctcc ttcacccatc acaaaaccat
    20161 tccgattttc gtcaaacgga atcgaaccac gattaggatc agttgattct gagagtgctg
    20221 ttaaagctgc aaatccagca atccccatct cacaaatcgt tgcttctgtt cctccagcaa
    20281 gaatgacatc agaatagcca tgtttgatgt tacggaatgc ttccccaatt gaattcgttc
    20341 cgctagcaca agctgttacg atagctgtac aaataccttt tgcacctaca cgcaaagcaa
    20401 tatttccagc tgccatgttg gcaatcgcca ttggtacgaa cattggagaa actcgttttg
    20461 cacctttttc atgtagacga ataacttggt tttcgattgt tggaagacca ccgatcccag
    20521 atccgatgat gacgccaaac cggtcttgat tcattttttc tttatctaaa ccgctcatct
    20581 ccattgcttc taaagcagcg tagattccat agatcgaaaa catatccatt cgtttgctat
    20641 cttttttaat aaaatattta tcgtaaggga aatctttgac ttcacccgct aaggtaatcc
    20701 ctgtttctga agcatcaaat ttagtgatcg gtgcgatgcc attcgtcccc tcttttaagc
    20761 tattcaaaaa gctgtctgcg tcatttccaa taggtgaagt gactccatag ccagtaatta
    20821 ccactcgatt catttgtttg ctctcctttt cttatccgtg catgaccaat ccgccatcga
    20881 cattgatgac ttgtccggtg atatatggat tttgtgctaa gaaaactgct gtttgtgcaa
    20941 tatcttctac ttgtccgaaa cgctgcatcg gaatttgctt ttccgcttgt tccttgactt
    21001 tgtctgctaa aacttccgtc atatcagttg tgatgaatcc tggcgcaatg gcattacatg
    21061 taatgcctct agttgcagct tctcgtgcca cagattttgt taatcctact acgccagctt
    21121 tactagctgc ataatttgct tgaccaatat ttccaatcaa cccagataca ctagagagat
    21181 taatgatggc cccttctcgc tgcttcatca tttttttcaa tacgtgctgc gtcatattga
    21241 aggttccaat caaattgata tccagacatt ttttgaaatc ttctgcatcc attcgcatca
    21301 caagtttgtc atttgtgatt ccggcattgt ttactaacac atggatcgat ccaagctttt
    21361 cttctgcttc tttgatcatt tgtcccgctt tctcataatc agagatgtca ccggaaactc
    21421 ctacgcattt tacaccgaat gcttcgattt cttctatctt ttcttttgga atctctccgc
    21481 gaccattcaa aatgatattt gctccagctt ttgcaaaagc taaggccatc gctttgccga
    21541 ttcctcttgt actacctgtg ataaatacat tttttccttt tacatccata acgttcctcc
    21601 ttatagtatt gctattgttt catcaaatgt ctgcttattc tcaacacgca gaactggtat
    21661 tgttttatcg atttttttca taaatccgct taatactttt ccggggccga cttcgatgac
    21721 ttgttcgatg ccgattgttt ttaacttatg gatactctca tagaaacgaa caggttgcat
    21781 gacttgctgt tcaagtagtc ccgcgattgt ttctttcttc attatttccg ttgttgtgtt
    21841 gcttatgata ggaaaagaag gttcagagaa ttgaatctga cttaggtcct tcgccagttt
    21901 ttttgctgcc ggttctaaaa tagctgtatg aaaaggcccg ctgacattta gagggatcat
    21961 tcttttgaac cctttttctt ttaatagtaa aacagcttcg tctactgctt tttcttcgcc
    22021 accaatcacg atctgctgag gtgtattata atttgcagga gatacgatgc cgtacttgct
    22081 agcttcatga cagctttcct cgatcgtttc aatcggcgca ttcatcactg caaccatttt
    22141 cccgcttcca gctggggcag cttctgtcat ataagcgcct ctttttgcta ctaacgcaac
    22201 tgcttcagtg aaggatagcg cattactagc tactaatgcc gaatattctc ctaaactcag
    22261 tcctaaagct gcatctggga tgattccatg ctccttcaat aagcgataat acgcgatgct
    22321 gactgttaga atagctggct gtgtatattg cgtctgatcc aaacgttcat tttctgtaaa
    22381 gcaaagttca gccatatcgt agccgagaat ctcacttgct tcatcaaatg tctgctttac
    22441 aacggcttct tcatataaat cttttcccat accttgatat tgtgcacctt ggccactaaa
    22501 taagaatgct gttttcatgt ttttctccaa tatatccaga atatatgttt gattcttcct
    22561 attcaaaaag gccgagacat tcctatttca ggaaatgtcc cagcccccaa cctaatttta
    22621 atgattatgc agtttgtttt tctacgtaag tcactaaatc ttgaaccgtt ttgattccgt
    22681 cttctgtttc gattttcacg tcaaattcat cttcaatttc attgatgatt tggaataaat
    22741 ccaagctgtc agcttctaag tcttcttgga tgtcagtagt caatttgatt tcttcagttt
    22801 cttttcctaa ttcttctgcg atgatttctt gaattttttc gaataccata ataatatatt
    22861 cctccgatat tgtttgcttt ttatttttat aatttaacag tctacaactc catgagaata
    22921 cttccccatg ttaaacctcc gccatagcct gtaaatatta ctttctgctg ttttcctaag
    22981 gtgatctttc cactagaaac tgcttcatcc aataagatag ggatgcttgc agcagatgta
    23041 ttcccatatt tatccatatt ggtcaggaac ttttcttgcg gcatctttac ttttcgggca
    23101 attttctcaa taattctgag gtttgcctga tgcaaaagca gataatcaac tggtttgtct
    23161 ttcatgactt cttggatatt gtttgcaacg tctcgtacgg caaaatcaaa aatatctctg
    23221 ccagtcattt gcaaataata cgaaccttct gaatcttctg aatagaacgg atttttattt
    23281 tgctggtagc cagcggtcaa tgagctactt ctggtaccgt cagctgctaa tttttctttc
    23341 agcaattttt tctgtggatt cgcttctaag agaatcccac ctgctccatc gccaaacaac
    23401 acagcagtcg tacgatcaga ccaatcgatt agcttagata gtgtttcacc accaatcacc
    23461 agtcccttag ttgagccgca gcggatcatt ttttcagcca tgcttaacgc ataaacaaat
    23521 cctgagcacg cagcactgat atcaaaagcg atagcatgag ttgctcccaa tttcccttgt
    23581 acttgtgctg ctacagatgg tgtcgtgtaa tcgggcgaca ttgttgctac taaaataaaa
    23641 tccaaatctt tagggtttag atttttctgt tttatcaatt tttccgccac taaaatgcat
    23701 aaatcagaag tctcctgatt tgttacacat cttcgttcac gaatacccgt ccttgacgaa
    23761 atccattcat cagaggtatc catcatcttg gaaagatcat tattagtaat aatcttttca
    23821 ggaaggacgc tggctgttgc agtaatcatt ccaaattgtt ccattaaata tcctcactta
    23881 tagtcttgca agaagtcgtg caagtttttc agtgcatcca gcaaggcatg ctgttcctcc
    23941 gtagccattc cgtctaagat gttcttgacc atttcacgat gaaaatgctg atggacccga
    24001 aatagtaact ttcctttttt cgttagtccc aactttacaa cgcggcggtc atcttcactg
    24061 cggatacgtt cgacatagcc tttttttact aacttgttaa tagcagtggt caaggtccca
    24121 acagtaattg atagttcttt ggctacttct gaagttgttt ttttcttgta catcccgatt
    24181 gcctcgatcg tgtgcatctc tgtgattgac agatctttga attgagattt tttcagctca
    24241 gactcttcga ttgtcaaaat atcattgaag acactaacaa gataatcatt tactgttttt
    24301 aaatctggtt ccatgttcca ccgtccacta attagtttga ttatcaaatc ttttgatgtt
    24361 caaactatat cttaactttt ttcaaaatgc aaccttaaaa gatcattttt ttactatatg
    24421 agcttttttt aagattttac tcatttaaaa caactgataa atcagcattt ataatttctt
    24481 ttattagaaa aatcatctat ctcacatatt ttgattatca aagtattttc tgtgaacaga
    24541 actatagaat tccctaaaac ttgtccatta tttcaaaatt tgttatagtc tttttattca
    24601 aagcgtaaaa ggagatccta tgttaaaaga aaaaatgaaa actctcccca tgctgccggg
    24661 tgtctatctt atgaaagacc gagatggtaa ggtcatctat gttggtaaag caaaacggct
    24721 caaaaataga gtaagcagct attttcatca gaataaacag cattcaaaaa aagtattacg
    24781 tatgattcat catataacag attttgattt cgtcgtagta gacacggaat tagatgcttt
    24841 attgctcgag tgtcaattga tccaacatta ccgtcctttt tataaccgac aaatgaatta
    24901 tttcagtaat tataattatg tacatattac caataaggga tttgtattaa cagatactcc
    24961 tacggctcga acatacgggc cttttcgtct ctataaaaaa atgccgtcta ttttgcgtat
    25021 aatggaagaa acttatcaaa tgccttggtt gtctgaaatt agtttgttag ctttgcgagt
    25081 ccagcttcct gacctacagg aaatgacttt tgaacagaaa aaaaaggaac tccaagggct
    25141 gtttcaaggc cgaaataaaa agctactgac ttacttgaaa aaaagacaac aacatttcat
    25201 ctatcagttg aatttcgaaa aagccggtat gcttcaaaaa gatatcgaat tagttactta
    25261 ttttattaga cggatccagg aacaaaagca gtttttaaga acaccatcgc tcaccttcag
    25321 catgccatta gcagcagatg agagtcagaa aaagcactac ctcgtttgtt atggtcaact
    25381 tgcagaaaca atgattacct ctggtgacgt ttctcctgac ttctattatg aaaagaaaga
    25441 agctcattta tctttgaaac gtcaattgtc aaaagaagaa atcgatcctg tacaaatctt
    25501 gatcagttac cgcaaaaaat tagaaaaaga acagtttgaa attgaactgt taaacaaaaa
    25561 agaagctgaa aagcaactaa attagttgct tctcggcttc ttttaggcat cttcaacttc
    25621 aaatgtcaat tatcctaaaa tttattggat taagtcatag atttccatcg ctacgatatc
    25681 gatattatca aattcgtatt gctgatgagt atctgtttca gttaattcaa atgtatcggt
    25741 cgctttatca taagttactg tacacttctc tacgccttct ttttcaaaac ggcgaacttg
    25801 cagttcatta tctgctgact cgaccatagc ctccagtcgt tttatgattg cgactaattg
    25861 tgaatgtgtc atcggaggtc ctccttttct tatttctgtt atattgtatc aaaagcatag
    25921 cctacttgca ttgattttct ctaaaaaaac tcgtttttta tgattttttt tctggtattt
    25981 ttccttcagg ccaccaaagt ttgatcaaag cttgggttcc gtcattctca aaacctttat
    26041 ctgccaactc ttcatatagt ttcaatgctt gggtagttgc tggcaataca agatccattt
    26101 tttctgcttc ttctaatgca atctttaaat ctttgataaa atgtttgaca aaaaaaccag
    26161 gtgtgtaatc ttcttttaaa atacgaggag aataattgct taaagaccag ttagctgcac
    26221 ttccacctga aagcgtctca atcactttct ttaaatcaag tccgtttttt tgtgcataga
    26281 caagcatttc cgtcattcct gtcatcgtac ctgcaatcat gatctgatta gccatttttg
    26341 tatgctgacc tttcccagca gaaccatgca atgtaaatgt cgtaccaaat tctttgaata
    26401 aaggcagtac ttgatcataa acggcttctt ctccaccgac catgatcgtc agtgtaccat
    26461 tctttgctcc taagtcacca ccggataccg gtgcatctaa cgcatctgcg cctttttctt
    26521 ttgctgtttt tgcaattttt tctgccaggc ttggtgtact tgtagtcaaa tcaactaaaa
    26581 tatgtcctga gatatctgca gaaaaaatcc ccgactgtcc atagtagatt tcttctacat
    26641 ctttggggta acctaccatt gtaaagagaa tattactttg ttctgctacc gcttttggtg
    26701 tatctgccca gatagctcct tctgttacta aaggatcagc ttttgctttt gtccgattat
    26761 agacagctac ttcgtgtcct gcttctagta gatggcgtgc gacagcactt cccatgacgc
    26821 ctgttccgat aaaaccaagt ttcatttttt cgacctccta aaaatcaaaa atccttgctt
    26881 ctatgctagt atacgctaga aacaaggatt ttttcataaa aaatgtttga attttatctt
    26941 ttaaatgaat ttaattttat tttgattcgt tcaatcgagt cttcttcttc ttttcttcgc
    27001 atctcttctt catgataaac aaccatgtct tgataaaatt cgatcatagc ttcgccgaaa
    27061 tgtgtagatg aaatatcata aagtttagct tctaaaactt ttggatcttt aggaacttca
    27121 ctggaaatat aatcaataag tgctggtgca aagtcttcgt cggtttcaaa agttttccct
    27181 aatgaaggat gatcgaacaa tcggttcaaa taagcatttc cttccacgac acaaggtgtt
    27241 ccggctgcca tcgcttctgt ataagtaagc ccttgtgttt ctgaagtaga agcactgaca
    27301 aaataatctg ccgctttata ataaagtgct acatcattgt tgtccacttc tcctgtgaac
    27361 tgcacaaatt cttctaactg cagttctgag atcaattcct ctaactcttc tacataaggt
    27421 ccttttccta cgataactaa tctggcgtta ggataagcgg agatgacctg aggaaaccca
    27481 ttaatcaagg cttggatatt tttctcataa gaaatcctgc ttaaagaaag aatcatcaaa
    27541 tggttttcct gaagacctag tgactccctt aatttgctga tatcctgctg agtgatatca
    27601 gaacgtttga atttttcaat gtcgatacca gtaggaatca cgcgaagcgg tgctattaca
    27661 ccatagctgc gtaatgtatc aataacacgt tcacttggac aaactacacc tgtcgtatga
    27721 tttgcaaata atctagacaa gtattttaca tgggattgcc tgatcacttt tcctttaccg
    27781 atataatgaa ggtagtcttc atacatcgta tggtaagtat ggatcagagg tattttcaac
    27841 ttcttagcta ccatcttccc aagtatccct gctccgaatt ctgtatgggt atgaataaga
    27901 tctagttcta gttctttggc aaccaaataa gcataccaca tgcctctaac aacaactctt
    27961 cgatccttga aagaaataaa tggtacactt ggcattcgaa tcacatcttt ttccaactta
    28021 tccgcattag gatcggtcgt tgtaaaaata tagacttcat gcccatgtct ttctaattct
    28081 tgttttaatg ttttgatcga tgtagcaact ccgctgactt gcggaaagta ggtatcggta
    28141 aaaaagccga ttttcatgtt tttcgcctcc aaactagtcc ttgacctgat ttactttttt
    28201 taatttgcta gagacttttt cattcagtac actttcgtat acatttttaa gttcttcacc
    28261 gattttattg atacttcgct cttttgctgt ttgataaccg gcttgtgtcg tactcggtag
    28321 ttcttgattg gtaatctgtt ggatcagtcg gataaattct tcgtttgtct tccccatata
    28381 gcagttcttg ctatcttcta accagccatt atacactgga atatctcgaa caagtacgtt
    28441 ttgtttgctt gctaaagctt ctaaaacaac gatcccttct gtctcttcat aagaagggaa
    28501 aaagaataga tctgcacctg aatacgcacc ttctatgatc tctccacgaa tgtaaccagg
    28561 aaacaggaca ttttcaggat gatcttcttt tacaattttc cggatgttcc gaggaatcga
    28621 gtacatggga acatgaccaa accaaataaa tgtgtattcg ggcatcttct tagcaatttc
    28681 tacgaaatca atgattcctt tacgttcgaa aaacaaaccg acacaaatga ttactttttg
    28741 atccggagac agtttgaaat attctctgaa cttttgttct ttcattggat cagggtgata
    28801 tttctctaag tcgatcccat tggaaatagc ttgaatcggc acacggatac catagctttc
    28861 caacaactgt tttgaataag gtgttggcgt gatcagatag tccgcttttt ggtacaatcc
    28921 taccagatat ttttttacga taggagaaag ctggttcgat ccgataaagg aatttcggaa
    28981 atcttcctct gtagaatgcg catgatagac aacttttttt ccttctcttc tggctttatt
    29041 gaccatatta tgactattga ttccgtatgt attgatgtgc aggatgtcat aatcttcttt
    29101 ttcatctagt gtataagaga taccgacttc cgtcagagca cgtttttgat gatcaagcgc
    29161 acgcccgatt ccagattttg caagcatctt ttcactttca aaatataata ataccttcac
    29221 cttacgtccc ccttcactta tatagtataa catagcctac aaactgtcac tcaaacttaa
    29281 gccctatttt t
//
LOCUS       DACDSY010000015        27946 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDSY010000015 DACDSY010000000
VERSION     DACDSY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.015"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="IWK44_000607"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="IWK44_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP9549163.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IWK44_000608"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IWK44_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP9549164.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="IWK44_000609"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="IWK44_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP9549165.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            1642..2274
                     /locus_tag="IWK44_000610"
     CDS             1642..2274
                     /locus_tag="IWK44_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9549166.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="IWK44_000611"
     CDS             complement(2276..3181)
                     /locus_tag="IWK44_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9549167.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3919)
                     /locus_tag="IWK44_000612"
     CDS             complement(3266..3919)
                     /locus_tag="IWK44_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9549168.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="IWK44_000613"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="IWK44_000613"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP9549169.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="IWK44_000614"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="IWK44_000614"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP9549170.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="IWK44_000615"
     CDS             6168..7253
                     /locus_tag="IWK44_000615"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP9549171.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="IWK44_000616"
     CDS             7250..8314
                     /locus_tag="IWK44_000616"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP9549172.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="IWK44_000617"
     CDS             complement(8563..9366)
                     /locus_tag="IWK44_000617"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP9549173.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IWK44_000618"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IWK44_000618"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP9549174.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="IWK44_000619"
     CDS             complement(10244..11251)
                     /locus_tag="IWK44_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP9549175.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="IWK44_000620"
     CDS             complement(11258..11902)
                     /locus_tag="IWK44_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9549176.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="IWK44_000621"
     CDS             complement(12002..12760)
                     /locus_tag="IWK44_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP9549177.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IWK44_000622"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IWK44_000622"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP9549178.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IWK44_000623"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IWK44_000623"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP9549179.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IWK44_000624"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IWK44_000624"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP9549180.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IWK44_000625"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IWK44_000625"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP9549181.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IWK44_000626"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IWK44_000626"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP9549182.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="IWK44_000627"
     CDS             18158..19027
                     /locus_tag="IWK44_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9549183.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="IWK44_000628"
     CDS             complement(19153..20052)
                     /locus_tag="IWK44_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9549184.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="IWK44_000629"
     CDS             complement(20217..21041)
                     /locus_tag="IWK44_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9549185.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="IWK44_000630"
     CDS             complement(21052..22347)
                     /locus_tag="IWK44_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP9549186.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="IWK44_000631"
     CDS             complement(22363..22842)
                     /locus_tag="IWK44_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP9549187.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="IWK44_000632"
     CDS             complement(22848..23876)
                     /locus_tag="IWK44_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP9549188.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="IWK44_000633"
     CDS             complement(23893..24999)
                     /locus_tag="IWK44_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9549189.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IWK44_000634"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IWK44_000634"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP9549190.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         7882 a         6131 c         5040 g         8893 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaa
//
LOCUS       DACDSY010000016        27740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDSY010000016 DACDSY010000000
VERSION     DACDSY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.016"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            714..2630
                     /gene="ltrA"
                     /locus_tag="IWK44_000635"
     CDS             714..2630
                     /gene="ltrA"
                     /locus_tag="IWK44_000635"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9549191.1"
                     /translation="MERDKTMTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSN
                     FKNLMKFIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKD
                     YRPRKVKRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNC
                     STENAIADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRK
                     MLKAPIVMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHY
                     RKNGAWDKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERL
                     KLPISMEKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIK
                     KIQYAGAKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARG
                     MTKNGIYGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRK
                     LIHQEQQAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEI
                     ECHHINPYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTY
                     RKKAANFPISIKMN"
     gene            2747..3547
                     /locus_tag="IWK44_000636"
                     /pseudo
     CDS             2747..3547
                     /locus_tag="IWK44_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            3544..6075
                     /locus_tag="IWK44_000637"
     CDS             3544..6075
                     /locus_tag="IWK44_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549192.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            6125..6457
                     /locus_tag="IWK44_000638"
     CDS             6125..6457
                     /locus_tag="IWK44_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549193.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            6458..7051
                     /locus_tag="IWK44_000639"
     CDS             6458..7051
                     /locus_tag="IWK44_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549194.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            7214..8908
                     /locus_tag="IWK44_000640"
     CDS             7214..8908
                     /locus_tag="IWK44_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9549195.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNGATCSAVKSCGLQVV"
     gene            9596..11473
                     /gene="ltrA"
                     /locus_tag="IWK44_000641"
     CDS             9596..11473
                     /gene="ltrA"
                     /locus_tag="IWK44_000641"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9549196.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            11604..11981
                     /locus_tag="IWK44_000642"
                     /pseudo
     CDS             11604..11981
                     /locus_tag="IWK44_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            11981..13186
                     /locus_tag="IWK44_000643"
     CDS             11981..13186
                     /locus_tag="IWK44_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9549197.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            13202..13846
                     /locus_tag="IWK44_000644"
     CDS             13202..13846
                     /locus_tag="IWK44_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549198.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            13857..14663
                     /locus_tag="IWK44_000645"
     CDS             13857..14663
                     /locus_tag="IWK44_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549199.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            14685..14915
                     /locus_tag="IWK44_000646"
     CDS             14685..14915
                     /locus_tag="IWK44_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549200.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            15461..15736
                     /locus_tag="IWK44_000647"
     CDS             15461..15736
                     /locus_tag="IWK44_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549201.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            15778..16200
                     /locus_tag="IWK44_000648"
     CDS             15778..16200
                     /locus_tag="IWK44_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549202.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            16219..17811
                     /locus_tag="IWK44_000649"
     CDS             16219..17811
                     /locus_tag="IWK44_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549203.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            17823..18158
                     /locus_tag="IWK44_000650"
     CDS             17823..18158
                     /locus_tag="IWK44_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549204.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            18305..18499
                     /locus_tag="IWK44_000651"
     CDS             18305..18499
                     /locus_tag="IWK44_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549205.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            18531..20903
                     /locus_tag="IWK44_000652"
     CDS             18531..20903
                     /locus_tag="IWK44_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549206.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            20964..22424
                     /locus_tag="IWK44_000653"
     CDS             20964..22424
                     /locus_tag="IWK44_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9549207.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVANKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVI
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEVISLKD
                     ICREVAKELKDCERSNGTYDFDRFYQGKGDINSQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            22435..24639
                     /gene="topB"
                     /locus_tag="IWK44_000654"
     CDS             22435..24639
                     /gene="topB"
                     /locus_tag="IWK44_000654"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9549208.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            24885..25478
                     /locus_tag="IWK44_000655"
     CDS             24885..25478
                     /locus_tag="IWK44_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9549209.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            25494..26393
                     /locus_tag="IWK44_000656"
     CDS             25494..26393
                     /locus_tag="IWK44_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9549210.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            26396..26881
                     /gene="ssb"
                     /locus_tag="IWK44_000657"
     CDS             26396..26881
                     /gene="ssb"
                     /locus_tag="IWK44_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9549211.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            27247..27507
                     /locus_tag="IWK44_000658"
     CDS             27247..27507
                     /locus_tag="IWK44_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549212.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT        10404 a         4483 c         5756 g         7097 t
ORIGIN      
        1 tgtgacctgt ttagcctaag gataaaagag gctagttcca acgaacattg gaataacttt
       61 actatcgaga aatcaaatga cggggtaacg ccctgaaggg tttccactga tactgcgaca
      121 tgcgttagta ggatgtgcat ccaaacgcgt atgaagctcg ctgaagtcgg cagaagggta
      181 ccgtaaggaa agacacgata cgtatctacc acgcaagtgc tctagaggta gggtatgtac
      241 ctgatatgtc gggtgtccta aaaagttcaa taaggtgaga atgatttgat tatctgacaa
      301 acttgcgaat ggaatgtctg aaacgaataa ctcatagaaa tgtgaggggg aagggggata
      361 cgtcctcttt ccattgactc gaggttgagt aaaaatgtgt ttctatgaaa aaccttgcat
      421 ggttaaaggc tatggagtag tcaacaggca aaagcaagca cctaagttga aatctatgga
      481 tagatagtaa ggaacagggg aagcatctag cacggaggga gcataccgaa gatgtgtttc
      541 taaggaaaaa ccgaaatcgg ttataactta ggtgaataga tgtgaaagta gagccacgac
      601 cagtgaaaat ttcgtaatga aattggagga acaggctcaa gtcgttattt ataatataag
      661 aaacctattt aaaatcatgg attctcgtaa gactaaaaag gtcacgaacc agaatggagc
      721 gtgataaaac catgactaac acaaaaggaa ttagcttaaa gaacaaacaa ctcagacacg
      781 ccgaatacta cgacatgact gagactttcg atgaattgca cgcaatgagt gttgcaaaat
      841 caaattttaa aaatctcatg aaattcatta ttgatgacaa taatatcttt ctagcgtata
      901 gaaatatcaa aaagaataaa ggaagtgcaa cggccggata cgatggtgtc actataaaag
      961 acattgaaaa attggcatgt ggtgactttt taactattat taaaaggcga tttaaggatt
     1021 acagaccaag aaaagttaaa agagtagaga tcccgaaacc aaacggaaaa cttcgacctt
     1081 taggaattcc ttcaatatgg gacaggatag tccagcaatg tattctacaa attttagaac
     1141 caatatgcga agcaaaattt aataagcata gctatggatt cagaccaaat tgttctacag
     1201 aaaacgccat agcagattgt cttgctagaa tcaatcaaag taagatgaat tatgttgtag
     1261 atattgatat aaaaggattc tttgacgaag taaaccatac caaacttatg cgtcaaatat
     1321 ggacaatggg gattcgagat aaacaactcc ttgtaattat tcgtaagatg ttaaaggctc
     1381 cgattgttat gcgaaatggc aatgttgttt ttccaaccaa gggtactcca caaggaggga
     1441 ttttaagtcc actgcttgcg aatatatgcc tgaatgaatt tgattggtgg atagctaatc
     1501 aatgggagga acgaactgtc aaagagctga aacaccacta tcgaaaaaac ggagcatggg
     1561 ataaaggaaa tatctattcg aaattaagaa gccacacaag tttaaaagag atgtatctgg
     1621 tacgctatgc agatgatttt aaaattttct gccgaacagg agaagaagcc caaagggctt
     1681 tttatgccac acaaaaatgg ctcagagaaa gattaaagct cccaatttca atggagaaat
     1741 ctaaagttac gaacctaaat aaagctgaaa gtgaattctt gggtttttca ataagactcc
     1801 acaaaaaggg aaaaaagaaa gtggctttca cgtacgtttg tcgcaaagca gaagaaaaca
     1861 ttaaaaataa actaaaacaa cagattaaaa aaattcaata cgctggagca aaaaaaaata
     1921 ggcaccgagc aataggtgac tacaacatga tggtcatcgg tatccacaat tactatcgga
     1981 tagcttcaca ttgtagtgtc aatttcactg agatttatag aagtgttagg acagtaatag
     2041 aagcaagact gaaagctaga ggaatgacga aaaatggaat ctatggacaa gatcatgaag
     2101 gataccgtcc gtatctaaag tctaagcaaa tgagatatat tgaaggtttt ccgatacttc
     2161 caattggcta tattaaaaaa agaactccac tcaacaagcg gagaaatcta aataaatata
     2221 ccaaggaagg gagaaagctc attcatcaag agcaacaagc tatagaaagc tggaaaatca
     2281 agtatcttgt aaaaggtttt agtaacaata agaaatacac ggtggaattt tctgataaca
     2341 aaatatctaa gtttatttcc caaaaaggga aatgcgctat cacaggaaac gaacttttga
     2401 tggaagaaat tgaatgccat cacattaacc catatcattt gtccaaagac gatagttaca
     2461 agaaccttgt cattatctct agagaaatcc atagattaat ccatgcaact agtgaagaaa
     2521 ctattctgaa actgcttcgg ttactgaaac taaatcacta tcaaattaga aaactaaaca
     2581 cgtatcgaaa aaaagcagct aattttccaa tatcaatcaa aatgaattag ataggaaaca
     2641 aattatattg taattaacga tggaacgccg tgtgcggtga aagtcgcacg cacggtgtgg
     2701 accgggggaa aagacggaga taatatcaaa ctcttaccta tcggaattgc caccaatgga
     2761 taatatggaa ggaatcatga cggtcacagc tggccgaaac atgttgtggg atctgtttat
     2821 tcaatcttat cagcaacttt atgcgaagta tggagaacaa aacgcagcga ttatcaaaga
     2881 aaattgtcag acacatattt atattatgtc gacagatgat aataccatcg aagaattttc
     2941 taaaaaagtg gggaataaga cggttgaaca agagaacagt accatcaatt cattaggtat
     3001 gaatactcat atcaatcgca atgttgattc agatcgtatc ttaactcctg aaagaatttc
     3061 tactttttta gaaggtgaaa cgattgtact cgctccttta aaacgacaag atttgaaagg
     3121 tcataaaata cggccgtatc ctatttttaa tcatggggaa acgaatcttc cttatgctta
     3181 tcaatatatt gaagatttca atccatcgtg ggatatcaat gatatagaca tacattgtac
     3241 acatgcggac ttggatctta aggctttaca aattgattat gattatttcc taccagatga
     3301 atatgctaga aatctctatc atggactgca tgatcaatta gaagaaaaaa acgaagaaaa
     3361 tatgttttat aaaatcgttc aatctcttga gaatgagacg gctaaaaaat tgttaattca
     3421 attttacgaa gaacaagcag cagatcgact tcaagaatat gttgaaaagt tgatcaaaaa
     3481 tgaagaaatt tctgaaatga ttgggaaacg gttgttattg gaatctgaaa aggtggtgaa
     3541 agaatgatca ttggaggaat tttttctggc atagccgact ggtggtcagg cttattcgga
     3601 gaaggggatg caaaccccaa aaccgtaacg aaagtcttac aagattatgg taattatctg
     3661 cagtatcaag atgtgttcag ttggatttgt aatacttttt tatgggggtt gatcaaaggg
     3721 ttatataatc tcaatacttt actagaaaaa accatttatc aaagctttga tttaaaagac
     3781 ttattgaacg ccgctggggt aaatgaacta tttcaaagtt tgattagtaa ggtattggct
     3841 cttttttgtg tcgtcacatt aatttattta ggcttgaaat tttttatgag taaacaccca
     3901 ccaaaagtca aaaatatctt ggttaattta tttatggcca tgatcttgat ttttggtggt
     3961 agttctttaa tcgatgaagg gttaaatatc agtaaaagct tttacggaga cgtcactgca
     4021 gcaaataaag cagataaaac gggttcacct gcttttcaaa ttattcaaaa taatgtgatc
     4081 gatgtcagta cattactagc aactgacccg aataaagtag aatccctgcc aacagacaaa
     4141 agaaatgctt taacagcgaa aaattttaaa acagcgaata tcaatgggat tattactcct
     4201 aaacaagcta aagaaatggc ggataaagat aagagtggtt tatcagattt acaaaaggat
     4261 cggttaagag cgttacaata ccgactggaa ctggatgata atggaaaaga aattcctgtg
     4321 caaatcagtg atgaaggctt agccaaatat gtttatactt cagggtatcg tcgatacatt
     4381 agtagtccag ggattatttt agctggtgaa tcttctttgg cggtggccta tttatttatc
     4441 ctatttacga tcgttacttg cattattgag ctggtattta aaaaattcta tttagtgatt
     4501 gccgcgtcaa cggactttga gacaggacaa cggatgaaaa gtgctatcga agatatttct
     4561 caatccttct tgttattggc atttacaatt ttagaattac gaatctatac cttaatgctt
     4621 tcaggtattg gtgatttgca tgcggcagga aagatcaatg gattcttgta tgttgtcgct
     4681 ttgattgtac taaccattgc gctattcaaa ggctctcaat cagttacgaa aattttcggc
     4741 gttgatacga gtctgaaaaa cggcagtaat tctcttctta gcatgtttgc cttaggaaat
     4801 attgcgaaaa atgtgggtag cggagctaaa aaccttgcaa gtgctggtaa gagtggttta
     4861 agccatttaa atgatattcg taaaaatgga tttggccatc gaaaagatgt tacccctgat
     4921 aacggggaag aacaatcaga cgataaagct acaactgtac ctaatagtaa gaaaacagtt
     4981 tccgaaatgt tcgggaaagc gaaaaatggt gtttctaaag ccgcagaagg tcttggttac
     5041 gtcaatgaac gtgggttaaa aggaacagct gaagatctta cagaaaaagc caaagacacc
     5101 ttagaagata aattagacgg cacgagagct aaagaattgg ctgaagctgt gaagaatcca
     5161 tccgaagcac tagaaaaaca aaaagaaaaa gcttcagaag tcaaagaagg actaaaagat
     5221 gcgtatggtg aaggacaagt caatgcagca gtgaaagcta atacaacaac cgaaaaagaa
     5281 aatcaagaag gtatagtgat tccttcagtc aaagatcacc aagaagcgtt gcactctaat
     5341 gaaaattcga ttgaagagaa aaattccata gaagaaagaa attcagaaga aggaaaaaat
     5401 ggacagttac caaaaactga tccagaacaa tatcgctata aacctaacag tgaccttgaa
     5461 aatgagaagc gaatttcaga tttatcaaaa gtaaatcaag aagacaaagc agaaaatgag
     5521 ggacagttac caaaaactga tccagaacaa tatcggtata gatctgttaa ctccgaaaat
     5581 gaaaaacgca tttcagattt accaaaacga aatcaagaag tacaaacgga tgaggattcc
     5641 atcgttctgc caaatgataa tggaatcgaa gttggaactg aaaaatcaac gaaaattggt
     5701 ttacctaagg cacaaaattt accaatgaaa gatagtcgtt tgaatcaatt caaggataat
     5761 gatcaaagtt ttgttcatca atcacctgca ggtactgttc aaatgaaacc aagtcgtttg
     5821 cctgaaaatg gaaatctgac aactgtttct tacaatttat ctgaaagtgg tgagacaggg
     5881 aatgcgtccg ttcaaaatgt tgtgacaaga gaagctgcac caaaacctat tcaaaacgtt
     5941 catacaggtt acaagccagc aaatgtggca tttactgcac caacaaaaat aaatgtgaca
     6001 agagaaacta aaaacaggaa caatgcattg aaagaacaac tattgaggga tatcaaacag
     6061 gatatcaaac cataaaaggc ctacaagtta ggccttttct ttatatgaaa aggagaaatc
     6121 aattatggat aaatatataa acatgaaaaa ccctccaaga tttagtggag aatggaagtt
     6181 tttcgctttt ttctttttag atctattaac gcttggtggc tgcttttttt gggcgaaaat
     6241 ggtcaataat cttgtccact taccactctt ttttgcactc ttgaattatg gcttatggat
     6301 tgcgatagcg ttagtgattg tttggcgacc ctataataac ccaggtcaac gacaaatttt
     6361 tgcgattatc gagtctattt tttttgcgga caagaaccat tatcatgcgt tagatcctaa
     6421 ccgaaattat catggtacgt ataaggagga tgaataaatg cgagtcaaag gacaaaattc
     6481 agttgaaagg aaatccattt tgcagtttgg aaaaccagaa gagcgctcta tcttggactt
     6541 atttccgtat aaatcctgta cagaagaggg agtcttaatt actagagaag atcattttca
     6601 acgttattat cgtgtagttt ctacagacgt ggaaggatta aatgaacaag agaaactaga
     6661 acgaatgaat cagttgacca ccgtcatgcg tacgtatgtg ccaacaatta agttagttag
     6721 tttgaccaca gagacggatc taacggaaca aatcgtgcaa aagcggcagt tattagataa
     6781 aaatcgtatg gaacagtcct taggaagaaa tctacagcga ttaagaaaat atgaaaagaa
     6841 attagtagag gaaatccagg aattgaaacg agcagaacag gaacgccctg atttaagctt
     6901 ctttttcgtc attgaaggaa agacaatcaa ggatttgcaa accagaaacc gtcagttaat
     6961 gcgatctagt ggcattttag ggttaaaacc actctacaaa aaagaattga tcaagattct
     7021 ttatcgaatg aataatatga atgatgagta ggaatgggga agagagaatg aaatatccgt
     7081 tgatatggtt agaaaaagat gttccgaaag aagagaccac tagaaaattt cctcacactt
     7141 taagcctggg taagttggga aaaggaaaat cttttaacca agtaaagaat caaacaagaa
     7201 gaggtgactg tacatgttag aagcaattaa aacagctttt tcaccacaaa atgatcaaaa
     7261 ggtacttgat ccattaaagc aactaggaaa tcgggaaata gaaattttaa aatccaaagg
     7321 gtatgattta gactttttaa aacaagtgat gccacaaggt ggattaaatt tcgatgaaga
     7381 gtatgtgacc tatggaaatg gttattcacg atgtgtgaca gtttatcact acacaaaaaa
     7441 tcctacgctt ttttggttgg ccaatctcat gaacaatcct catacagtgg caacactaga
     7501 tgttagcaca gatgaaaagc aaaaagtgat tgatcaaatc aatcgtgcct tagatgaatt
     7561 aaatgatcgg tcagataatg aacgaaaagc gactgaccga aataaaagcg cggaagaata
     7621 cctagaacta gcggaatatg cccgaaaatt gacttctaat ggggaaattt ctaaacaagt
     7681 caaagtgaga gtacatgttt acggaaacac gattcaagaa gtagatgaac gagcacagga
     7741 actgatcgaa aatctcaaag ggatggatta tcgtgcagtg acctatctgc acaaaacgaa
     7801 aagagactgg caatcgatgt tcaccatgtt cgatgaacaa gctgaatggt ttggaaatat
     7861 caaagggcaa tcactaccat cgctaaacat tggcggaggt ttcccgttcc accaccaaac
     7921 gctaaaagat ccaggtggaa cacaatatgg ggaaaccatg acaggcggcc cgtttgtttg
     7981 gaatgccacg tataaaaata gtgtgcggtt gtctaccaat accttgcttc taggaatgat
     8041 gggagctggt aaatcgaccg ttcttaaaat gatcgcggaa gcccatttag ctgcaggaga
     8101 ctttatttgg ggctttgaaa agggaaaaga ctttattccc tttttaaagg aatataacgg
     8161 cattatggtg cgtttagacg gatctgacgg gatgatcaat cctttagaaa tatttgccac
     8221 acgtacgtat gatgaagctt ccagtttata tgacgatgga tcagtcaaag atttgaagat
     8281 caatgaagca gcgtcctatc aaacgcactt agacaaagtg gtctatcagg tacagttagt
     8341 gagtcctcag ctaaaaggaa cgatgaaagc agaatttaag acctatttaa atcgttttta
     8401 tgaagagtat ggaacagtac cacgcggctt tacttcctca aattcgcgtt caaatactga
     8461 tacgcaagtc acaggtaaag atccagaagc ttatccgacg ttcaaagaat ttttagactt
     8521 tttaggacaa ctggaattac caggtgcttc ccaagagaaa aagaatcgaa aagaagaaat
     8581 ggaatccatc gtagaatcct tatgtgagac gtatggcatg atttttgatg gtcatagtac
     8641 gattcgacac cttgaccaac aacagttggt cttttttgac attgactcta ttacaggctt
     8701 agatcgaggg gtacaacaat gtcaaatgta catggctatg acgttgattt ggaatcaggc
     8761 attgattcaa ggaagaagac aacgacgttt aattaaagaa aaacaaatgg aagaacaaga
     8821 cttcaaatgc ttcttggcga tgattgatga gtgccataat ggtgcgacct gttctgcagt
     8881 gaaaagctgt gggttgcaag tagtctaaaa actattgtaa taactcttat aacgagaagt
     8941 caaatgagtg ggtaacgcca caaagggctt ccactgatac tccgacatgc aacaagtagt
     9001 ttatgcagac caacgttgtg tgaagctcgg tgaagtcggc tgaaggatac cgtaaggtaa
     9061 aagaaatagt gaataccacg aaagttctct agaggtaaag ggtgtatccg ataagtcggg
     9121 tgtccaaaaa aacttaatat ggtgagtatg tacaaacgta ctgacgaact agcgaatgta
     9181 ctgtccgaaa ggcatagctc ctagaaatgg gagagacagt gagcaaaaaa tcactcattg
     9241 tgctaacttg tggttgagta aaagtcgttg ttatgaaaga ccatgctgcg ttacaggcgt
     9301 aggagaagtt agggggcaaa agctagcatc taagttaagg catgtggata gatataaggg
     9361 aacaggggaa gccatcaaca tggagagtgc atactcgatg aagtggtagt aagaaatatg
     9421 tgaatgacaa ataacttact ttagtgatgg tgaaagtaga gtcacgactg atgaaggttc
     9481 tgtaatggaa ctggaggaac aggctcgatt cgtctgtaaa agtcatgaaa cccgttcaac
     9541 catgggattc tcgtatgact aaaaaggtca cgagccaatt ggagcgtgat aaaccatgac
     9601 aaaagaaaca agtccaaaag ataggaagtt gcggcatgca gaatattatg gcatgacgga
     9661 aatcttcgat aagatatact ccaaaagttt gaagggagat aatttcaaga atctaatgca
     9721 gattatcatt tctgataaca acattgtcct agcatatcgt aatatcaaga gaaatcacgg
     9781 tagtgtgaca agaggagaag atggagtaac gattaaagat attgaaaatc tcaaccgtaa
     9841 tgaattcgta gaaattgtta ggaagcgatt cgcacattat aaccctcgga aagtaaggcg
     9901 tgtagaaatt cctaaatata atggtaaatt aagaccgttg ggtattccat caatatggga
     9961 tagaatcgcg cagcaatgca tcttacaagt attagaacca atatgtgaag caaaattcaa
    10021 taatcacagc tatggtttcc gcccaaatag atcagcagaa cacgcaatag cagattgtat
    10081 ggcccgaatg aatcaaagtc acatggaata tgtggtagat gtagatatca aaggtttctt
    10141 cgacgaagtc aatcatacta aactcatgag acaaatctgg tctatgggta tacgagacaa
    10201 acagcttctc ttaatcatta ggaaaatgtt aagagcaccc gtagtacttc caaatgggaa
    10261 gttgatatac ccaacaaaag gaacaccaca aggcggaata cttagcccat tacttgcgaa
    10321 catcaatctc aacgaatttg actggtggat agcaaatcag tgggaggaaa aagatgccaa
    10381 agagctaaaa catcatttta ggaaaaatgg agcttgggat aaaggtaata tctatcacaa
    10441 gctcagaaag agtacaactc taaaagaaat atacctagtg agatacgcag atgattttaa
    10501 aattttctgt agaaattata atgaagcgaa aaaagttttc tatgcaacta aaatgtggtt
    10561 acaggagcgt ctaaagttac caatatctga agaaaagtca aaaatcacaa atctttcaaa
    10621 agaagaaagt gaatttttag gctttacgat gaaattagtg accaaaggta acaaaaaggt
    10681 tgttaatacg catataggta caaaagcaca aagcatgatt aaagtgaaac taaaaaaaca
    10741 aatcaaacga attcaaaaaa gtcctaatag caaagatacc tttatctata ttggagtcta
    10801 taacagtatg gttattggga ttcataatta cttcaaaatt gcgactcatt gtagtgtagc
    10861 aatgcgaaaa attgcaagag aactcagacc aagttttgaa atacgactac gagaacaagg
    10921 aatctcaaaa gaaggacaat acggaaagta cgaaggatac aagccttacc tacggtcagg
    10981 acaaatacga tatataagag ggtttccaat attaccagta gggtatattc aacacagaca
    11041 tccgatgtgt aaaaaaagaa atattaataa atacaccgaa gaaggtaggg aagccattca
    11101 taagaagcaa caaaccgtag aagaatggaa gatatcgtgg ttgtgtagca atccagtgct
    11161 tagtaagaga gcaacgattg aatttgcgga caatcgaatt tcaaaattca tcgctcaaaa
    11221 gggaagatgt gcagtaacag gtgaggagct tatcttatct gaaatgcatt gtcatcatat
    11281 tatcccttat catgaaagca aaagtgatag ctatgaaaac ctagtgattg ttactgagga
    11341 ggttcatcgg gtaatacacg ctacacaaag tgaaacgatt gaggaacttc taaaatatct
    11401 aaaattaaat ccaaaacaaa aagaaaaact caatgagttg agattaaaag ttggtaacga
    11461 agaaatatct taataaatat ttgtaggacg ggaaaacaga atgtctttta tagacgaggg
    11521 aacgccgtat gcggtgaaag tcgcacgtac ggtgtggact gggggaaaag acggagataa
    11581 tatcaaactc ttacctatca gtatcgatta atccagattt tattgaaacg gtaaattatg
    11641 tcacaaactt tcaacgagaa atgcgtaaag tagaagcaat gactgtttta gctacacaat
    11701 ccccacaaga aatggtgcca gaaaatatgt cttctgataa cttctcgaaa ctgaagaaag
    11761 tctttgaatt ttcttctacg aagatcttta tgcgtatgga tgaatctatt ttacgtcata
    11821 tagaaggatt agttggtaaa tcaatgacaa actcagaatt agaatccatt cctcaacaaa
    11881 atcaaggaga cgctgtgatc aattttggct caaaagaaac cattcgtgtt cattttagtc
    11941 cgaacaaacg tcaattaaaa ttgtttgatg gaggaaaata atggaaaacc aaaatgaaag
    12001 tttaatcaag cagtatgtta agcgtcgtgc aaagcgacgc ttatttttgt ggttgtttgg
    12061 tacttctgca ggtttgatta ctattttgat tactgtgttc gtgacccttt ttctaatact
    12121 tgcggcgggt tcgattgata attctgattc agattcaagt agtggtggcg aagcttttac
    12181 aggagaatat tcggaaggct taccgattta taaagaaatc aaaggaagag gccctttttc
    12241 tgatgagatt gcgcaatatg ctgtaggtgc agcagtgaaa tataaattat taccaagtgt
    12301 gatcctttcg caatacggtt atgaatcagc gtttggtacg tcggcgtccg caagaaatga
    12361 tctcaactat tttgggatta cttggtttga tggttgtctg tttcctaaag gaactgcgcg
    12421 aggaatcggt ggtattgaag gtggctggta catgaagttt cccaactcaa aagcagcctt
    12481 ctcctattac ggctttatgg tagccacaca atccaatttt aatgcatgtg taggaaataa
    12541 aagccctggc gcaagcttat tgatcttagg acgaggtggt tatgccgctg caggaattac
    12601 tgaagatagt ccgtattata caggttgtat gtccattatc acttctaata aattaaccga
    12661 atatgatgaa ttcgccatca aacattgggg tgaaggtggc aataacaacg gcacgatcac
    12721 tggtgaatgg acgaatccat tccctggcag cagtttagat aaaagttcct tcagtggtgg
    12781 tcaattattt ggaacaaatc caggcggtga attccgtccg aatggcttcc atgatggatt
    12841 agactttggt tcagtcgatc atccaggaag tgaaattcac gcggtgcatg gaggaaaagt
    12901 cgtgtacgtt ggcaatcctg gtatttcagg tttaggtgca tgtgtgatcg tgattaatta
    12961 cgacggtttg aatatggtat atcaagaatt tgcgaatagt acaggaaatt ctcgtgtgaa
    13021 ggttggcgat caagtgaaag ttggtcaagt aatcgggata cgagatacag cacacttgca
    13081 tttaggtttc actcgaatgg attggcgtca agcacaagga cacgcattca cagacgatgg
    13141 tacatggatt gatccattac catctttgaa tagcagcaaa aaatagatca ggagaaaaaa
    13201 tatggaaaat aaaactttaa aaatcgtctt aacagctgca attagcgtta gtttaatcgg
    13261 caatatcgcg ttgttttggc agctgaaaga agaacaagag acaaatacct ttaaaattga
    13321 agaagtggct aaaaacgcgt ctgagaagct caaaaaaaat tcctctgagg cacaggcgaa
    13381 gatcgacaag ttaaatcaaa ccatcaatga gaaaaaccaa gaaatcgatc agctgaagca
    13441 acaagatggc acaaaagcaa aagaggaagt aactgaagca caaagaaaga cggcagaaga
    13501 gttcggtcaa ttggccattg ataaatcatt aagtagaaat gaattaacgg aaaaactaaa
    13561 agatgtcgca acccaagaag tgatcgacaa attaagtcct gcagacgatg atcaccaaca
    13621 tgatgattac ggaagttact cctttacatt aggtgacgtg caaacttatg ctcaaacaag
    13681 cagtgtaaaa gaggaacaaa attttgtcgt atttgttgat tatacaatcg gcaatccgca
    13741 attcaaagat gtcaaaccac aaaaaataaa aggtggatta actgtcactg aaaaacaagt
    13801 agatggtcaa tggaaagtca tagactttag ttatttcact cgttaggagg aagaaaatga
    13861 gtaagaaaaa aggagaaaga caacgtttca aaaaacgaaa aagttatctt caatggctgg
    13921 gaatctttat gattctcggc tttttgattc ttgctagtcc gatttatacg aatcagaagg
    13981 tagatatacc agatgaggta agcacgaacg cctttttaaa cacgaaagag ctgacaatgg
    14041 tttcaaaaga aaaaggagcg aaccagcaat tagtttttga atttgcgatc gatgatgaag
    14101 atcaaagctt gttaaaagaa ctggctaatc ttaattatcg cgtagaagta aagacagcta
    14161 aaggggatta tcaagcaata cgagccaaag tgatcaaggt gtctgatgat tattttgcag
    14221 tgaagctaac gcatgtacca gaagaatata tcgccatgcg tttaaccatt attcctgaaa
    14281 aaattgatcc aaaagtggat atgcaagaac cacaagatct gatttattac gtgcacgaag
    14341 ataaggttaa agatcaagtg aaagatgaag attatgaaca acacgcgatc aattacaaag
    14401 tgaaaggata caagaaagaa caaaaagcag ctcaaaaaca aatggaatct ttccaagcaa
    14461 ccattgattt gaatgaagaa ttagtgaaga agctaaaaga tcaattgcct tatcaagtcg
    14521 cagatgatca ggaaaatact gaaaacaaga ttaatagcta ccagcaagaa attgaaacat
    14581 cgaaaacgca actgaaagat caagaagaga tcattcaaaa acttcaagaa aaaattgatc
    14641 gtatgacaaa agaaaagatt tagaaggaca ggtggagtga aaaaatgaag cgaacaaact
    14701 tggctgacaa agatactttc gctgagcaga aagtattgat taaagcctat ctcaaagttg
    14761 taaaagctaa agaaatatgt cgagaagtga atttatctaa cacaacaata tctgagttga
    14821 agaatggtcg aaagaattta gatactttaa aattcagatc atttgaagct ctatatgtag
    14881 ctgctaaaaa atattttaca gctgataaca aataaaaaac aggagtattt tatgtgaaaa
    14941 aagtatataa aaaatgctta tgaatcagct atatcacctt aaccatttgt taatgagatg
    15001 ggagaaaaca ttaaccacga ttctaacgca aacatcaagc acataaaaca tttaacactc
    15061 ctcctttctg gctgaagatt tcaaccaaac aggaagacag cgtatcaatt atagcataca
    15121 ctttttctta aattcatttt tcatatgaca aaatatttcc ttctaatcga caaagttaga
    15181 aggatttttt attgtctaaa tacacaaata accttaattt ttaaagagca taaggaaacg
    15241 tgatatcact ttctcacgaa aggtgttagg taggctgtgc ctaccacgtt tctaagaaag
    15301 ataaatgata tcaaaatttt ggatttgtgt gtagatttgt gggtaggatg aaagcgaatg
    15361 tgggcagtaa aataagcgtt tgtgtgtagc aaaatcaaaa aatgtgtagg gatatgtgta
    15421 gctacacata tccctacaca taataaattt ggaggaaaat atggacttat taattagaaa
    15481 tattgaagaa aagtatatta ataaaattga caaacgttgt gaagaactta cggtcaagac
    15541 tggcaaaaaa tggagtcgaa atcaatacct aaaagttttg atcgaaaatg attttgatca
    15601 cgctcttctg aattataaaa aagaccaatt tgatcggttg ttagaaaaat ttgtggatat
    15661 tcaaacttat aatacgaaaa ctttagaaga atatattgca acaaataatc aattaattgg
    15721 tttgttaatt gaataataaa aatagtattg tgaatttata aaaggaggaa atcaattttg
    15781 gcaaaagcaa caagaaaaca tgtatatctt tacgatatgc atatcgaagt acttgatgaa
    15841 ataatttcta ctcatgaaga tatatctaat tatagtgaag cagtcagaca tttgtgtatg
    15901 atgtatgatc caaaaatcga agaaaaaata aatccaattc aaattaaatt gaatgcaatg
    15961 ggaaaagaaa tttctatttt aaccgaaatg gtttcagagt ttgcggatct tcatttgaaa
    16021 gatacaggaa tactaattgg tacaaattca actgtttatc aagaagcaga agaacgtgtg
    16081 gaaggaaaaa ttaaatccaa tcaaaccaaa aaatattctt ctaagttaaa gaaaccagtg
    16141 aatgaatctg ttcataccag agtagatcca tttgatccaa cgaagattct aaaaagataa
    16201 aagtagaagg agaacgtgat gaataaatcg cctgcagtta ttcttacttg tcaatttacc
    16261 ttgcccaata aaaaatcttt ttcaacatac attgattata tgacaagaga aaaagcactg
    16321 gaagaaatgg aacgacgtac gcccgaagaa gaacaggaac tgcaacgaat caagaatgct
    16381 ttagaggatt tttatattcc tgaaggagaa actttttcgg aaagtaagac ggataaagaa
    16441 ttcagtgaaa cgtcgaatga agctcaatcg ttgatggaac atggaatcaa ttttgacaag
    16501 ttagaggaac aagattttac caaatatctt tcttatatga cccgtcacta tgcgttagaa
    16561 caaaagaaag aactgacgca aagggaacaa aaagaatacc aacggttgaa ccaagcaatg
    16621 aaaaaatatc aaacagagcc acataaaaaa gcttcaggaa atcttcctgg agttttttct
    16681 atggcttcag acgaagtgaa aggaagtgat ctgaaggaga tcaaacaaat cttccaaaga
    16741 ggacaacaaa aaggctcaat catttatcaa gatgttatct ctcacgacaa ttcgtatctt
    16801 gaaaaaatgg gcttgtacga tcccaaaaca gataccttag atgaagaagg tttgaaagca
    16861 gctggaagaa aaatgatgga gacattattc gagaaagaac aattgaatga aacaggttac
    16921 tggatggcaa cgattcaccg taacactaag catatccata ttcattttgc agttgttgaa
    16981 aaagaaaata cgcgtaaacc gtatactgag attgaaaatg gtgtgagtta tatcgtaccg
    17041 aaggggaaga gaagacaagc aacattagac gcaatgaaaa ccaattatgt acatgagtta
    17101 gaacgtttct cttatgaaaa ggatcgaatg atcggtatgg agaaacgaaa tttattgaca
    17161 cgaaaaagtg atttgcgtaa caccttaaca aaagtagtaa aagaaccagc acgttacgat
    17221 cgacacgcca tgaacttatt aaatgaagta tataaaaccc tacctgaaaa acgttcggaa
    17281 tggaattatg gagatgaaaa tcgaaccaag ctttcaccat ttacaagggg aaaacttgat
    17341 gagttaacca aatatgtttt aacaaaagaa cctggatatc aagaatatat acagatttca
    17401 aaagcattga aagaagaagc caaaagcctt tatggggaaa gcaaaagaga aacaaagaat
    17461 gcagaaaaaa acgccctctt tgatttaaaa aagcgcacag gaaatgcgat tttaacagaa
    17521 ctgaagagac aggatagtca aataaaacca ttaatagaaa gtgtcaatca gatgcaatct
    17581 atttacaatg aacgatcctt tcgaacactc aaacagttgc caacaaatga atacaagtat
    17641 agttatcaag aattcgtaaa gcgtaggaaa caattccaac aaagagttgt cacgcagcga
    17701 agcttgcgtc aattaaatcg aacaattaat gcacatgaag aaaaatacca cgcaagtaaa
    17761 gcatatgaag agcaacagcg aagaatccaa atggaacaag aaagacaata ggaggaagag
    17821 aaatgaaaca atatttagat caatggaaag tcatagaagg atcactaaaa gaagaacgaa
    17881 tcgaacaact tcctgattgt ttagaaaaag agcatttgtt tcagataagc gaaatgttga
    17941 gaaacgaaca gtttgatcca aatcaatttt tagttgttga gtacccagct actggtgtgt
    18001 attgttgtaa tcatgtgaag ggtgaaaaat attttattat tcaagaatac gaaggtaagt
    18061 tagcgcctta ttatactact tgggaaataa atgaagaggg aatcaataat ttcccatgtg
    18121 aaagcattga agagtctatt tctctcacgg aatgttaaat aaatagagac gtaaaagagt
    18181 cctgaacgtg attgtccagg actctttttc aatgaattgt tttccgccaa ttcactgagt
    18241 ggtgcgattc aattttatta tatcaaaaga aagtgagatt ccaaagtgaa gggaggaaga
    18301 agaaatgact cagacaacga ttggtgttgt ggttgaaaaa agcagggata atctcgtttt
    18361 tgtgacagaa attcaaacag gtagagcttt tgtagtcaca gacaaagctg caaaagcgta
    18421 tcaaaatggc aatattttaa cgttaaatct gactacgaag atgttcgttg atgcagtaga
    18481 aaattatcca tttgtttaga caaacgatat tttacaagga ggaaacggac atggcagaag
    18541 aaaaagaacc actagataat agtcgtttgg gaaagtccaa aagaaaatta gtacggttgc
    18601 aaaacgaatt aaataatcaa tttcaaaagc tatttgatca ccaaaaattg acaaatgggc
    18661 aaccaatgaa tgacaaacga aatggtcgtt catggtttaa ccagcaagaa aaaatagaaa
    18721 ataaaataca aagtttgaga gaagagatca agcaacaaga aaaacaggta gaaaaacttg
    18781 aaagacaaga agagctaaag gaaatgggtt ataacaagta cggtggcctg gacatgacga
    18841 tcgaaaatat tccaagaatc aaagaagaaa tcgaacgctt tgaaaaaggt gaatcgactt
    18901 tttcagctgc cacgatccga aaataccaac gaaaattgga aacattagaa cagctgaaag
    18961 aaaaatcaga gaaaggaaaa gaaaatcttc ttcctgaagt acaagcgatc attgacagcg
    19021 gacgagtgac gcaatggaaa aagaatccaa ctatctattt tctgaaaggc tatcgaaaag
    19081 tcgctttaga actcaatgaa aacgggggat ttgaggaatc taaaaaatac caagccacgt
    19141 cggaagaaga aaaggcagct gttcgtgaat tacttgaaga agctacacct actgattctg
    19201 tgaaggagaa agaaaccgat catttctatc gagtagaatt caacgaaaat gatccagcaa
    19261 aagggttgaa aagttatggt ggggagatcg taacactaga attgatcgat caactcaaaa
    19321 cactggataa ccaatacgcc gcagaagcag gctattataa attctatttt gaagaagtga
    19381 agaatggaga agtagtcgat cagatacgaa tggatctagg ggatggcgtc aatagtaatc
    19441 accccatcta tttagagtta gctagattgt gcgaacaatc acctgaaaag gaacaagaac
    19501 aaacagtcaa aaatgaacct atttcaaaag tacaaaacga gattaaagag acaaaaacaa
    19561 aacaaaaggg ttatcaaaaa cgaacggcag aagagattaa gcaagaagtc aaatcacttt
    19621 cccaacaggc gcttgatgcg gtgaaaaagc atactcattc acctgaagac gtcaaagaac
    19681 ttttgaattt tatgtctcgt tttccagaac gatcctttag aaatcaatta ttgatcgagg
    19741 aacaatttcc aggcgcaacg gcttgtttag gtcgtgccgc gttgaaaaaa gaaggaatct
    19801 atatcaaaaa aggagaacaa gggaacaaga tctttgtcag aaaaacggtc aaaggatttt
    19861 atgagaaagg acgtggcttt gttcgagaaa cagaggctac accagaagat aaaaaacgaa
    19921 tcgaaagtgg tcaaatcgaa gtgatcaaaa aaccttacta cacgattgaa aaagtgtttg
    19981 atatcacaca aacacaattg aaacctgaag actatccgaa aatcttcccg aaccgcgtct
    20041 ttgattttca attggatcaa acagggcaga cagaacttca agttgggatt caagcggtag
    20101 ccaaaaatat tggaattcaa attcgagata tgacagaaag tgaagtctat caaagagaat
    20161 taggacaagc acgtggggcg tacgttcgta acgaatttac tggcgaagaa gaaatcgtca
    20221 tgaatactcg aaatactcct acgcagcatt tagccacaag tattcacgaa ttagcccatg
    20281 ctagaatgca caaattttca gaattagata ctgcaacaaa agaactacag gcagaaatga
    20341 cctcctatat tgtgtgcaag cattttggga tggacacctc ggagaaagct attccttata
    20401 ttgcgacatg gacgaagaat agtcagacgt tggatgataa ggaagctgcg gaacgtggga
    20461 aaatcatgaa tgatgtcagt cgtgtcgcaa atgaattcat tcaaaccatt tctaatgaaa
    20521 tcaatcaata cagagaaaga gaaccagaga tccagccagc agaagtgaag caggagcata
    20581 aaaatactgt agaaacggca gaacaaaaga atattgtgcc cgttggctca ctttggatcg
    20641 acaagaaaga cgggaaagtc ctggaagaag acacgggccg aacgttacat ttgaaggatg
    20701 cggcgtttat tgaagatccg cagcataatc aagtgatttt aaaaggaaaa agttatggaa
    20761 aagagatcca aacggcgtta ccttcagaag aatttgcgct aggaaatcca ttccgatttt
    20821 cacttgagac agtgtcagaa gtggcaaaac gaaaaaatga aatctattat gaacaacaat
    20881 tgtcacaaaa agaacgcgta taaaacaagc aagaaagact atctgaaaaa tggatagtct
    20941 tttttctttt gggaggtttg aaaatgacag agaaaaaaat gagtctcatt gatcgatgca
    21001 agcaaatgga tattgtggat tttgcacgaa acaatggttt agcggtggcc aataaaggac
    21061 gagactatcg cttagaagac catgattcat ttgtttttga tcgaagaaaa caacgttttt
    21121 attggaacag tcaaaatatt agtggcgaca ttattgaatt agctaaactt tttttcatag
    21181 acaaagagat ccaagaccct aagcaacaat ttaaagcagc cactgatttt attctaaaga
    21241 atgaagacaa aacagaacga gtggaaaatc ttcattttga gacagaaaag tacaaagatc
    21301 accccgttga ttatcaacca ctcacagaaa aaggaaggaa ttatctgaag gaagaacgaa
    21361 aactgcccga ttggttgatt gattatgcag aaaaagaagg attgattgcc gaactaaagc
    21421 caaaacatga acggcaaaat ttcttagttc gagacgatcg tttggatcat gcggtcgcct
    21481 ttttatggaa agatccacag acaaaagaga cagtaggggc gagttatcaa ggaacgttca
    21541 ttgattatga acgttttggt gagagaggca catataagca tatagataaa aattcgacag
    21601 cgaatcatgg ctttaaccta aagattggtg atcctaaaca gttaaaattt tttgagagta
    21661 gtattgattt acttagctac gctgcactga atcgtgatca gttaaacgat acgtggttag
    21721 tctctatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag gcagtatctg
    21781 aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat aatgaccgtg
    21841 caggacatat tttctacgaa aaagaacaat taatgggcgc agttgatcca tttacgaatc
    21901 agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaga gaatacaaag
    21961 tcatttatga agaagtcgca aaagaagaaa aggtcacacc agaagctatt atggccatac
    22021 acaagacaga aaacaatctg caattaacga accaactggt ttccgctcac aaagtcaatg
    22081 cgtcttttgg tcagcagctt tctgttaacg attcaataga agtcattagt ttgaaagata
    22141 tttgtagaga agtcgcaaaa gaattaaaag attgtgaacg ctcgaatggt acgtatgatt
    22201 ttgatcgttt ttatcaagga aaaggggata ttaattctca gattcttttc tcttataaag
    22261 cagaacaata ctacaaaggc tataaaaatc atgaacatga atttgtacct gaagtgaaaa
    22321 aggattggaa cgatcaattg aagcatgaga tccatcaaca ggaaattaga aagcaaaaac
    22381 gtgcgatgtt gtttcaacaa ggcagacaac aagaaaggga gtgagatcgt cacgatgaaa
    22441 actgtcattt tagcagaaaa accgtcacaa gcgaaggcct atgccgatag cttttctaaa
    22501 gccacccgaa aagacgggta ttttgaaatc caagatcgat tgttttcggg tgaaactgtg
    22561 atcacgtatg gttttggtca tttagtcgag ttagattcgc ccgatatgta cgatgaaaat
    22621 tggaaacaat ggtcattaga gcatttgcct attttcccta cacattatca ctatcatgtt
    22681 cctaaagata agaaaaagca attcaacgtg gtgaaacagc aacttcaatc ggccgacacc
    22741 attattattg cgacagatag cgatcgtgaa ggggaactga tcgcttggac gatcattcaa
    22801 caagcaggcg ctgatcaagg aaaaaccttt aaaagacttt ggatcaactc cttagaaaaa
    22861 gaagccattt atcagggatt ccagcaatta cgagacgcag aagagaccta tcccaaattt
    22921 gaagaagcac aagcacgcca gatcgctgat tggttgatcg gcatgaacgg cagcccactg
    22981 tatagtttac tcttgcagca aaagggcata ccaggaagtt tttccttagg cagagtccaa
    23041 accccaacgc tttacatgat ttatcagctg caggaaaaga ttagaaactt ccaaaaagag
    23101 ccttattttg aagggaaagc ccaagtaacc gctcaaaatg gcatatttga tgcgaagctt
    23161 gatcccaatg aaacccaagc gacgcaagaa gcatttgaag cctatttaaa agaaaaaggt
    23221 gttcaagtag gaaagcagcc aggcacgatt cttgaagtcg aaaccgagaa aaaaagcgcg
    23281 gcaagtccac gtcttttttc tctatccagc cttcagtcta aaatgaatca gctcatgaaa
    23341 gctagtgcca aagatacttt ggaagccatg caagggttgt atgaagggaa atatctaagc
    23401 tatccacgaa cagacacccc ttatattaca gaaggagagc atgcgtatct gttggatcat
    23461 ttagatgaat acaagcattt tttaaaggct gaatcattcc ctactcctat tcatacaccg
    23521 aatagtcggt atgtgaataa taagaaagtc caggaacatt acgcgatcat tcctacaaaa
    23581 acagttatga cagaagcaac gtatgaaaag ctgacacctt tacaacaagc gatttatgat
    23641 caagttttaa agacgacggt ggccatgttt gcggaaaaat atacctatga agaaactacg
    23701 attttgacac aagtccagca gcttcagcta aaggcagtcg gaaaagtacc agtagatcta
    23761 ggttggaaaa agctatttgg caaagagagc gacagaaaag agaaggaaga agaaccgcta
    23821 cttcctaaag taacaaaagg agaaacggtg acggtagatc tacaaatctt tgaaaaagaa
    23881 accaagccac cacaacctta cacggaaggt acactaatca ccgccatgaa gacagctggt
    23941 aaaaccgtgg atagtgaaga agcacaatcc atattgaaag aagtggaggg aattggtaca
    24001 gaagcgacac gagcaaatat tattgaaacc ttgaagcaaa aggaatatat caaggtagag
    24061 aaaaataaac ttgtggtgac gaataaagga attttgcttt gtcaggctgt tgaaaaagaa
    24121 ccgctgttaa caagtgccga aatgaccgcc aaatgggaaa gctatcttac aaagattggt
    24181 gagcaaaaag ggacacaagc tacttttcta gcaaatatcc aaaaatttgt ttcccacttg
    24241 ttggaagtcg tcccaggaca aatccaagcc actgattttg gagcaacgtt acaagaggtg
    24301 aaagtagcca gtgaaaagca agaagcggcg cgtcatttag gatcttgtcc aaagtgccag
    24361 gaaaaagaag tcatacttta tcagaaggtg gcgacttgca cgtctgaagc ctgtgatttt
    24421 aaattgtggg taacgattgc caaaaagaaa ctcacagcga cacaactgaa agagatcatt
    24481 cagaacggcc gaacaagtca accagtcaga ggactaaaag gtcagaaagg ttcgtttgaa
    24541 gcaacgatcg ttttaaaaga agactttaca acaggatttg agttttctga aaagaagaag
    24601 accacctaca aaagaaaagt gagacgtaaa acgagttaga attgacggag ggaaaaacga
    24661 tgagaaatac aacgcataaa gaacaagaaa tcgaaaatca aaaagccaaa atacgttcag
    24721 agcaggagaa aatgcgtgcc agcaaacgaa gactacagca attgaagaat caacaacgga
    24781 ttcaaaaaga acttgaatta gatcgataaa tcctcctgtt gttaatatta aactaacatg
    24841 ttatagttat gttagtttaa tattaacatt cgagagggaa agcgatgaaa gataaagtca
    24901 agtgcatttt tgagaaatac aatggtaatc tatccttgaa ggatgctaga aatgaagggt
    24961 tatcacctat gacgttagat cgtttagccg aaaaggacaa aatagaaaaa atagcatcag
    25021 gtttttatac cttaaaagat agttttgtgg atgaattata tctcgctcaa agtatctata
    25081 ctagaggaat cttctctcac gagacagcgc ttgatctttt tcaagtagga acatatatcc
    25141 ctaaaaaaat taatatgatg tttcctaaag gatacaatgt ttcgaaagag aatttagatc
    25201 agtatgccat tcaaccgcat tacacgaata aagaaaactt tgaactggga atcacagagg
    25261 cagaaagctt ttacggcaat aaaattttcg tctatgattt agagcgaacg ttatgtgata
    25321 tgtggaatcc cagatataaa gcaagcgtag aagtaaaaca agaagcatta aaagaatata
    25381 tgacaagtac cagcagaaat acaaataagc taagaagata catgaatatt ttaaaatcac
    25441 caaaagaaat gactttgtat atgttgccac tttattgaga ggagtaaagg tgaatgacac
    25501 cgacacaatt taaagcgaaa acaagaaatg ttgcagctga gaaacattta aacccacaaa
    25561 ttgttcaaag atattatatg atggaaaaat tattagaatg tattgttgaa tcgccttatc
    25621 atgatgattt tgtattaaaa gggggatttc tgatcggatc aaaatacggg ttagaaaacc
    25681 gttcaaccgt tgatattgat acgacttatc gaaattcgaa actaacaaaa gaaaaattag
    25741 aaactgtttt ttctgaatta acagctgctc ctacaaaaga tggcattcgg tttgaattaa
    25801 agggcttaac agagacgagg gaagccgatt attacccagg ttttcaagca aagtttattg
    25861 cttttcttga aaatgcacgt gtacctttta aattagatat tacttcaggt gattctatta
    25921 ttccagcagc attgatatat gaacataaat tgatgtttga agataaaaca attcatattc
    25981 cagcttatcc tacggaacaa attttagctg aaaaattaca tgccacgttt agtttcggaa
    26041 tagataattc acgtatgaaa gatttctatg atctgtatat gatccctaaa ctagaacgta
    26101 tcgatgagat aaatttgtat aagagtgttc aaggaacatt taaggaaaga aataatcaat
    26161 cagtgtttaa gaatttgtat aatcaggaaa tgcctcaatt gatgaagaat gagggattga
    26221 gggagaaatg gaaaagatat cagcaagaaa actattttgc caaagatatt tctttcgaat
    26281 ctaccttaac ttctgttgat tcgctgatgc aatcaattgt cgagcaagag gaagtagaac
    26341 gaaaacaaaa ttttaagaga tcacaacaaa tgaatcaaga aatggagaga taaagatgat
    26401 caataatgta actttagtag gtcgattaac caaagatccc gatttacgtt acaccgcaag
    26461 tggcactgca gtgaccactt ttaccttagc cgttaaccgt aattttacta atcaaagtgg
    26521 caatcgagaa gctgacttta tcaattgtgt gatttggcgc aaaccagctg aaacgatagc
    26581 gacattagct aaaaaaggca gcttgatcgg cgtcgtcgga cggattcaaa cgcggactta
    26641 tgacaaccag caaggacagc gtgtctatgt gactgaagtt gtggccgata atttccaact
    26701 attagaaagt aaagcagcga cagaaagccg tatgcacgct gatcaatcgt ccagcagtcc
    26761 aagccctacc acctttgaac aacgggatac agcgacgcct aacaacaatg gattgaacgc
    26821 ttcacagaat ccatttggtg gacaatcaat cgatattagc gatgatgatc taccgttcta
    26881 agtagcattt tctattggtt gcatttgcta cggtttatgt tataatttag acaacaaaaa
    26941 gaattttcaa tctataacca aaaaccacta gctggcgggc tagtggcttt cttttacgtt
    27001 tctattataa gaattaacgg gtaggcttac tgtctttctt ggcaaattta caatcaagcc
    27061 atttgcagat atagtgcgcg gctacttctg ccatgattgc aaaaacaaaa tcaaaaagaa
    27121 ttttcaaacc tataacctcc tccccttgcc agattaggcc ggggtagcag acagcaagac
    27181 tattatagca taattgaaat atccaaatga ttggattttt gctataattt tagcgaggtg
    27241 attgtgatga acaaatatgt tggtttgctt gataaaatcc gagtaataaa aacgcaaccg
    27301 ttattggttc gctttacttt acaaacgatt catgaatcga ttaattgtgt agttgcagat
    27361 attgaaatta tcgataaatt gctaattatg gatgatggaa aatacaatat tgccgtcaca
    27421 ggtcacttca ataaacgaaa tcagctagtt attgctagca tgtatgttag aaatcccgat
    27481 cattttacgc gatcgatggg catataaata aaagaagagt tgtagtccta aatgaggatt
    27541 gcagctcttt ttgttttaca tagaaacagg aaaagacctt tttaggaaga cacaagtgcc
    27601 ttctcaattg aaaaaccttt ctctcactcc aaccgccacc gccgttcgag aagaaaggaa
    27661 gtgaacgctg cacgttcact aagatatggt gtcttgcaaa gcaagacaaa atagacaaaa
    27721 cttcctctca ccaaaaggtt
//
LOCUS       DACDSY010000017        27363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDSY010000017 DACDSY010000000
VERSION     DACDSY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.017"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..159
                     /locus_tag="IWK44_000659"
     CDS             <1..159
                     /locus_tag="IWK44_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP9549213.1"
                     /translation="SLRSLRIETFFRPMHNTQIRMAIVLFSIFLGYTASNFFLEVIAL
                     CRNLFVSL"
     gene            256..1557
                     /gene="murA"
                     /locus_tag="IWK44_000660"
     CDS             256..1557
                     /gene="murA"
                     /locus_tag="IWK44_000660"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9549214.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1570..1746
                     /locus_tag="IWK44_000661"
     CDS             1570..1746
                     /locus_tag="IWK44_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9549215.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1881..2177
                     /gene="yidD"
                     /locus_tag="IWK44_000662"
     CDS             1881..2177
                     /gene="yidD"
                     /locus_tag="IWK44_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP9549216.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2361..2828
                     /locus_tag="IWK44_000663"
     CDS             2361..2828
                     /locus_tag="IWK44_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9549217.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            2913..3797
                     /locus_tag="IWK44_000664"
     CDS             2913..3797
                     /locus_tag="IWK44_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9549218.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            3914..4147
                     /locus_tag="IWK44_000665"
     CDS             3914..4147
                     /locus_tag="IWK44_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP9549219.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4235..4315
                     /locus_tag="IWK44_000666"
     tRNA            4235..4315
                     /locus_tag="IWK44_000666"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4269..4271,aa:Tyr,seq:gta)
     gene            4322..4393
                     /locus_tag="IWK44_000667"
     tRNA            4322..4393
                     /locus_tag="IWK44_000667"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4354..4356,aa:Gln,seq:ttg)
     gene            4908..5615
                     /locus_tag="IWK44_000668"
     CDS             4908..5615
                     /locus_tag="IWK44_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9549220.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5675..6319)
                     /locus_tag="IWK44_000669"
     CDS             complement(5675..6319)
                     /locus_tag="IWK44_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP9549221.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6446..6733
                     /locus_tag="IWK44_000670"
     CDS             6446..6733
                     /locus_tag="IWK44_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549222.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6841..7161
                     /locus_tag="IWK44_000671"
     CDS             6841..7161
                     /locus_tag="IWK44_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9549223.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7242..8555
                     /locus_tag="IWK44_000672"
     CDS             7242..8555
                     /locus_tag="IWK44_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9549224.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8552..9271
                     /locus_tag="IWK44_000673"
     CDS             8552..9271
                     /locus_tag="IWK44_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP9549225.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9399..9950
                     /gene="raiA"
                     /locus_tag="IWK44_000674"
     CDS             9399..9950
                     /gene="raiA"
                     /locus_tag="IWK44_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP9549226.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10172..12706
                     /gene="secA"
                     /locus_tag="IWK44_000675"
     CDS             10172..12706
                     /gene="secA"
                     /locus_tag="IWK44_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP9549227.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12832..13933
                     /gene="prfB"
                     /locus_tag="IWK44_000676"
     CDS             join(12832..12894,12896..13933)
                     /gene="prfB"
                     /locus_tag="IWK44_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP9549228.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14038..14724
                     /gene="ftsE"
                     /locus_tag="IWK44_000677"
     CDS             14038..14724
                     /gene="ftsE"
                     /locus_tag="IWK44_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP9549229.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14708..15601
                     /locus_tag="IWK44_000678"
     CDS             14708..15601
                     /locus_tag="IWK44_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9549230.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15753..17201
                     /gene="cls"
                     /locus_tag="IWK44_000679"
     CDS             15753..17201
                     /gene="cls"
                     /locus_tag="IWK44_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9549231.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17538..18395
                     /locus_tag="IWK44_000680"
     CDS             17538..18395
                     /locus_tag="IWK44_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP9549232.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18510..19430
                     /gene="pstC"
                     /locus_tag="IWK44_000681"
     CDS             18510..19430
                     /gene="pstC"
                     /locus_tag="IWK44_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP9549233.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19430..20314
                     /gene="pstA"
                     /locus_tag="IWK44_000682"
     CDS             19430..20314
                     /gene="pstA"
                     /locus_tag="IWK44_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP9549234.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20329..21135
                     /gene="pstB"
                     /locus_tag="IWK44_000683"
     CDS             20329..21135
                     /gene="pstB"
                     /locus_tag="IWK44_000683"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9549235.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21151..21909
                     /locus_tag="IWK44_000684"
     CDS             21151..21909
                     /locus_tag="IWK44_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9549236.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            21922..22599
                     /gene="phoU"
                     /locus_tag="IWK44_000685"
     CDS             21922..22599
                     /gene="phoU"
                     /locus_tag="IWK44_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP9549237.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            22837..23868
                     /locus_tag="IWK44_000686"
     CDS             22837..23868
                     /locus_tag="IWK44_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9549238.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            23865..24632
                     /locus_tag="IWK44_000687"
     CDS             23865..24632
                     /locus_tag="IWK44_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9549239.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24732..26300
                     /gene="liaX"
                     /locus_tag="IWK44_000688"
     CDS             24732..26300
                     /gene="liaX"
                     /locus_tag="IWK44_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP9549240.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26600..26917
                     /locus_tag="IWK44_000689"
     CDS             26600..26917
                     /locus_tag="IWK44_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP9549241.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            26917..27267
                     /locus_tag="IWK44_000690"
     CDS             26917..27267
                     /locus_tag="IWK44_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP9549242.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
BASE COUNT         9049 a         4636 c         5921 g         7757 t
ORIGIN      
        1 tctttacgtt cgttacgcat tgaaactttt ttcagaccaa tgcataatac gcaaattaga
       61 atggcaatcg tacttttttc aattttttta gggtacactg caagtaattt tttcttagaa
      121 gttatcgctt tatgccgaaa tcttttcgtt agcctgtaaa ttttaaaaaa aggtactatt
      181 ttaaaagcaa ggatgttata atagaaaaga ttttgagtaa gtgtatgctc attggaattt
      241 tttggaggga acaacatgga agagatcatc gtaagaggtg gcaatcaatt aaatggaaca
      301 gtaagaattg aaggagctaa aaatgctgtt ttgcctattt tagctgctag tttgttagca
      361 gaagaaggta ttacgacatt agacaacgtt ccgattcttt ctgatgtctt cactatgaat
      421 caagtgattc gtcacttaaa cgtagatgta gattttgatg aacaaaagaa tcaagtaaca
      481 atcgatgctt caagacaatt agaaatcgaa gcgccttatg agtatgttag tcaaatgcgt
      541 gcttcaatcg ttgtgatggg tccgctactt gccagaaatg gacatgcaaa agtagctatg
      601 cctggtggat gtgcgattgg taaacgacca atcgatttgc atttaaaagg atttcaagct
      661 ttaggagcaa aaattattca aaaaaacggc tatatcgaag caattgcgga tgaattgatc
      721 ggtaacacga tttatttaga tttccctagt gtaggtgcta ctcaaaatat catgatggct
      781 gccgtaaaag caaaaggaac aaccatcatt gaaaacgtag ctcgcgaacc agaaatcgtt
      841 gatttagcaa acattttgaa taaaatgggc gctcaagtct atggagctgg tactgaaaca
      901 atgcgtattg aaggcgtgga tcatttacat gcagtcaacc actcaatcgt acaagatcgt
      961 atcgaagcag gtacctttat ggttgctgct gcaatgacac aaggcaatgt gctgattgca
     1021 gatgctatct ctgaacataa ccgcccattg atttctaaat tgatcgaaat gggtgcagaa
     1081 atcattgaag aagaaggcgg agtccgtgtc atcggaccaa aacatatctt acctacagat
     1141 gtaaaaacga tgcctcatcc tggtttccct acagatatgc aagctcaaat gacagctatc
     1201 caattagttg cagaaggtac aagtgtagtg actgaaacag tgtttgaaaa tcgtttccaa
     1261 catttagaag agatgcgccg tatgaacgct catgtcaaaa tcgacggaaa tgtagcaatc
     1321 atggatggaa accatgaatt gcaaggtgca gaagtatatg caacagactt gcgggcagca
     1381 gcagcattag ttttagctgg cttgaaagca aatggaatca cacgtgtgcg taacttgaat
     1441 tatctagacc gtggatatta taatttccat atcaaactac agcaattagg tgcagacgtt
     1501 gaacgtgtag acatggatca aacttctgct gaaaaaacag cacaaacgat tgcttaagga
     1561 agtggctgaa tgagtaacca acgatatatc ttattaactt tgataaagat tttagtggtg
     1621 atcttattgc ttatccttct ttttgtggcc ggaacgatga tcggatatgg tgtcatcggt
     1681 ggcggcaatc catttaaagt attccagcca agtttgtgga ttcatattcg agactttttt
     1741 cattgataaa taagaatcac agtggttcta gtcgatatga agtgagccat gaagcagaac
     1801 atgacaaaag aaaacagaag ggaacgttga cgttagtcgc gatccctttt tttagtaaaa
     1861 gaaaagaagg aatctaattg atgaagaaga tattgattta tttagttaga ggatatcagc
     1921 gctttatttc gcctgcattt ccacctagct gccgttatta cccaacctgt tccaattata
     1981 tgataaaagc aattcaaacc catggtgcac taaaagggac ggtaatgggg actgcacgga
     2041 tcttacgctg tcatcctttt gtcaaaggcg gtatcgatta tgtaccattg acattccaat
     2101 taacgcgaaa taagaaagat aaaaaagaac cagattataa aaaggttgtg aaaaatacga
     2161 gtagcactga gcgataaaga aacaatttgg aaaatagctt cttatatttt gcaaaattgt
     2221 gagttattgc cgaagtgttg gattttgaac accgtcaaaa aaaggttgtg aaaaatacgg
     2281 gtagcactga gcgataaaga aaaagaggaa aattattgtc aaagtaacaa ctccgcaact
     2341 acttatatgg gggacaaaaa atgatcgaag aactaaacaa gaaagattta ccatggtcct
     2401 tgttattaga tgcagatcct gataaagaaa aagttcaagt atatgtttcc agagggagtg
     2461 gctggatatg gaaagaaaaa gaaaaaacaa taggtgtact gatctatgtg gcaagggaaa
     2521 aagaatacga aatcgtcaat gtagcagtag ccccaacgta tcaaggaaag gggattggcg
     2581 gaaaattact tgagacagct tttcagaagc tttcacaact cgcttccagt caaactcaga
     2641 tcatcatacg aaccggcagt actagcagtg cggcgttaca tctctatcaa aaaatgggct
     2701 atgtggagat tggtcgagtc aaagactatt ttatccatca ttactctgag cccatttttg
     2761 aaaatggaga tcaattaagg gatcaagtaa ttttagcaat tcacttacct aaaaatagct
     2821 ttaactaata aactttcaat tcgagaaaat gctttcaaat gctaaataga tacattacaa
     2881 tagctttagc aaacaaaaag gagaatattt ttatgggaat attagttttt gattttggtg
     2941 gatcggctgt caaatatggt tgttgggatg gcaaagagat taaaggtaag ggacaatttg
     3001 caacgcctga atcttgggaa gaaatgaaag cgcaattatt tcaagtttat aaaaaaatac
     3061 tcgtttcttt tgaagtcgaa ggaattggaa tcagctctcc tggagtagtg aataatgaga
     3121 aacaggtgat tgagggaatc agtgcgattc cttatattca tggatttaat attttccgag
     3181 atttggaaac tctgtttcaa ctcccagtaa cgattgaaaa tgacgccaac tgtgccggaa
     3241 tggctgaatt ttatgaaggt gctgcccgtg attatcaaga tgttgctttt gttgtggcag
     3301 gaactgggat tggcggggcg ttgttcacga aaggacaaat caataaaggg gcacatcttt
     3361 atggtggaga atttgggttg atgttcttaa atggcgataa gacattcagt aaacttggta
     3421 cagcggtaca gatggcttgg cgttattgtg aacgcaaagg tctcgataaa aattcttata
     3481 ccggaaaaga tgtctttgaa ttagcagaaa ctggagatgc aatagcaaaa gaagaagttg
     3541 agagttttta tacgtatctt acaaagggat tattcagtat tcaattttcg tttgatccag
     3601 aagtgatcgt attaggcggc ggtgtttctg caaaagaagg gctcattgat gagatcaaaa
     3661 gccgaatgaa gaagctgaca gaacaattcg atctgcatga tttcgaaccg cagattttat
     3721 tatgtgaata cagaaacgat gctaatcttg taggtgcagc agcaaactat ctcgctgtac
     3781 gtcaagggga gaattaagag ctttagagaa ggtgcagctt gcataaaaga agtgaatgaa
     3841 atgaatagga gatttttacc taaggaatgt tttatttgct gcagtatgat aaaatatctg
     3901 agaggtgaaa atgatgtcaa aaaaaaataa agatgttgaa gtgcgtatag aagaagtgaa
     3961 aaaagatatc aaaggtaaga attatgaagt cactcagtta tttataggga aaaaaatgat
     4021 tggtgagatc ttagcctatg ggccaaaaga atatgaaatt ttctttggtg aagaagattt
     4081 tggaaaagaa aaaagtcttg aaaatgcgat agaaacagtg attcgacact ggaatctgca
     4141 cgaataaaaa aatatcaaaa aaactttcaa taaagggttg caaaaatctg gaaaattagg
     4201 tattataact aagtcaggtt gatacaaacc tttcggagga gtagcgaagt ggctaaacgc
     4261 gacggactgt aaatccgttc cttagggttc agtggttcga atccactctc ctccatttca
     4321 ttggggtata gccaagcggt aaggcaaggg actttgactc cctcatgcgt tggttcgaat
     4381 ccagctaccc cagtatacca caatcgtaag ctctcgttag ttcgagagct cttttttttg
     4441 ctttgctacc ttgttccttg aagattctgc tgatttaaga tagagttaag ttaactacaa
     4501 gaattggagg gatccacatg aaatggaaag aaaaagtaga cgaagcagct gaaaagttat
     4561 atgatttggt caaatcagaa aaatacaata ttgaagtgaa tattccgaaa aaaggcggaa
     4621 aagcggtcca ggtaaaatca aaacgcccaa cgaatcatac aaaaaaatgg atggctaaaa
     4681 accgctaata ataacaaaag atccataagt cggcattttc atcgatttta gggtcttttg
     4741 ttattttcta ggttgaacat atctaaaaaa ttgtttctga caataggagt aggaagaacg
     4801 atgtgtgtct aaatcctctg ttgatggata gaaaccttta tctttttcag ttgtgaatca
     4861 ggtatatacc agttataata gaagaaacga aaggacgggt gtacgttatg tcgaatcaat
     4921 tacctgtata tatacagatt catgaccaat taaaaaatga aatcgaacaa ggtatatgga
     4981 aagtcggaga ccgtcttcct tcagaaagag aattagcagt gaaattcaat gtcagccgta
     5041 tgacgttgag acaagcaatt caaaacttgg cagatgaagg aatcttagaa agaaagatcg
     5101 gttcgggtac atatgttgcg cgagaaaaag tacaagaaaa aatgtctggt gcaacaagtt
     5161 ttacagaaat tatggaatcg caaaatcgag taccgtctag tcggacaatc tcttatttct
     5221 tgacttcgcc aagctcaagt gaaatggaaa aactaaactt gagtaaagat gacacgatct
     5281 tgcgaatgga acgtattcgt ttcgcagatg atgtccctat ttgttttgaa gttgccagtg
     5341 tgccacaaaa gctgatcgat ggctacagca aagcagagat tactgaatcg ttgtatcgca
     5401 cgctagaaga aaaaggcggg aaaaagatcg gtccagcgaa tcaaacgatt actgctgtgt
     5461 tggcttctga gaaaattgct gactatttag aggtaaaacg aggagatgca gttcttcgtt
     5521 tgcgccagat ttcttatttt gaagacggta cgccgtttga gtatgtgcgt acgcaatacg
     5581 taggaaatcg ttttgagttt tatttggaaa aataaaaaac aggtgatccc tgattcctta
     5641 cttagaaata aaagagaatc agaatcacct gtttttatat atgatcagtt accagttgct
     5701 cgtgataaga aacaggaccc tcttcaaatt ggacttgtcc gttcagcagc tcaatgatgg
     5761 attctttaaa gtggtcagca cttttttcat ctacagcaca gatgacgcta acttgatcag
     5821 tatattggat ttcttttata aaaaaatgtt ggagttcaag ataattctgt aattttccta
     5881 gttgcggata cgtgatcatc acagagatct cctgttgcaa ggtaccttct acgattccaa
     5941 tgtcagacaa tgcttgagag acagcatgag tgtatgctcg tattaatccg cctgtgccga
     6001 gtttcgttcc tccaaaatat cgagtgacta ctgcagctac attgatcagt tgattttttt
     6061 tgaggacttc caacatcggt acgccagccg ttccacttgg ttctccatca tcgctgcttc
     6121 gctggatttc atttttttct ccaatgatga aggcgctgca gttatggttc gctttccaat
     6181 gttctttttt tatttcttga atgaaacttt ttgcttggtc ctctgattct attcgtctaa
     6241 gagaacagat aaagcgtgat tttttgatct caatctcact ttgtccattt tcttttattg
     6301 tacgatagcg ctcgatcatc ttgtcacctc tttttttcat tttctttatt cagtataaat
     6361 aaagttctag gttgtttcaa cactgtaacg gttgcgcaat cgctgtagta tgcgataata
     6421 aagacaaagg taggtggatc tggggatgca ggaacgaatg aaaaagtacg atgcaattac
     6481 gcattatttg aagaacaatg gcggctctca agtaacgttg acctttaccc aatttgacga
     6541 actgctgttt cccagtaacg gacttcccaa aacagcaagg gaaagtacag attggtgggc
     6601 aaatgattat aaacaccctg aaaaaggtgc ctatggctgg ataaatgcag gctatgaagt
     6661 agttgtcatc aatttggaca aagaatatgt cgtgttcaat aaattagtga aatctagttg
     6721 gctatttgat taaataaacc gcgtttagaa aacgttgaca aatttctttc tttttatata
     6781 atagagtaac agacgcgaag aaaacgtttg cgtagtttgt tttaaaaagg agagaaaagt
     6841 atggctaaaa aaacaatcat gcttgtatgt tcggctggta tgagcacaag tttactagta
     6901 acaaaaatgc aaaaagctgc tgaagcaaaa ggaatcgact cggatatttt cgctgtgtcc
     6961 gcttctgacg cagataacaa tcttgcaaat aaagatgtag atgtactgtt attaggacct
     7021 caagttcgct ttatgaaagc tgattttgaa aaacgtctgg aaccaaaagg cattccttta
     7081 gacgtcatca atatggctga ttatggtatg atgaatggcg aaaaagtgtt agatcaagca
     7141 atcacgctca taggtgaata aatagaacag tataactatc gaacaagcga tccaaaaaat
     7201 agatcgcttg ttttttcgta tattttttta gggcgaataa tatgaatcat ttttggggta
     7261 gaaggcttct gacgagagat attaaagcaa tagactgtga ggaggtaaat ttaccaaatt
     7321 atgagaagat cgcagcagtg gaacgtttgg gcaactggat tctttgtcat cgctgtggaa
     7381 tgaggacgtc cgtcattgat ggacagcttg cagattacgg ttatttttgt acacattgtc
     7441 tatatttagg gcgatgcgac actcaacaag atttatattt gtttgatcag cctgaagcaa
     7501 aacctcgaga agtcgtcttt ttttggacag gacagcttac tgaaaaacaa acggaaatcg
     7561 caaagagaat actttgccat tcagaaaaaa gacatcatct tatttgggct gtaacaggtg
     7621 cagggaaaac agagatgctt tatcctattt tagtggaaac tttaaaagct ggaggaagag
     7681 tagcgatttg tacaccgcga gtcgatgttt gtaatgaatt gtttttacgc tatcgttatg
     7741 tttttccaga agaaaaaatc acgttgctcc atgggaatac agcaacggct tataccttta
     7801 cttcttttgt tatctgtacg attcatcagc tataccgttt ttatcggtcc tttgatttaa
     7861 tagtgatcga tgagatcgat gcgtttcctt ataacgggga tgcaggatta gctcatgctg
     7921 tgcaaacagc gattaaacca gacggacgat tcatctactt aagtgccaca cctgataaaa
     7981 agttactaaa ggaaatcaag cagacgtttg acatccacaa acttgctttg cgctttcatc
     8041 ggcgtttact tcctgaaccc gtcttggttt tcatgaataa ttggcgacaa aaatgcaaga
     8101 acaagaagaa gataaaatct ttggtgcgga tcattcatca attaattaaa gaaaaccata
     8161 tccttttatt ttgtccaagt atcacgatga tgcatcgttt gaaagccaca cttcaagagg
     8221 ctcttccgag atatgagatc acagatgttt cttcaagaga caatcagcgg gcggaaaaag
     8281 tccagaaaat gcgagaagca aaatatgaca tactgttaac cacgatgatc cttgaacgtg
     8341 gagtcacatt tgaaagaata tccgttatcg ttttaggagc ggatcatccg gtttttacta
     8401 aatcttcttt ggtacaaatc gcaggaagag ccgatcgtaa agggccttat acgaacagtc
     8461 aagtatactt tttctatgaa gaaaaaactg cagctattcg aaaagcttgt caggaaatca
     8521 aagaaatgaa tgaagaaggg aagaagatgc tgtgaggtgc agttgctgca gtaaagatat
     8581 cgtccaaaat ttaacattca gcgagattat tggtgtttcg aaatacagat tacctcagac
     8641 ttgttcagtt tgtcgcgcac aatttcattt attggaaaca agaggctgtt ctggctgtca
     8701 aaaaccaatc catttcaatc tagagagtac gaaaaagaaa gtaaagtcta ttcccgttta
     8761 ctgcagtgac tgtcaagaat ggaagaagaa atatccgacc tatgattttc agcaccgtgc
     8821 cttttttgtg tacgatgaaa atatgaaggc ctggctcaat caatacaagt ttatgggaga
     8881 tatccgacta gcaggcacct ttgcatataa atggacgcag ataaaaaaag aatacccgac
     8941 atacatctat tgtccaatac ctttatctga aaaaaggcga acagaaagag gattcaatca
     9001 agtaagcgag atgttgaaag cagcaagaat tccttttcag atgctactca aaagagagaa
     9061 gcatctgctg gctcaagcaa aaaacacgcg aaaggaaaga ttggagatgc ctcaaccttt
     9121 tgaacttgca gtcacctttt ctaagataca aaataaaaaa atattgatta ttgatgatgt
     9181 ctatacaaca ggacagactc ttttccacgc agcagattgt ttgcaggtcg cacagccgaa
     9241 aatcatccga accatttctt tagcaagatg aattagatgt ggaaatttga tagcgattta
     9301 atttgcaaat aacctagagt tcgatataat agaattaaga agagggaaag agtggtcctt
     9361 ctccttcttg attgtggtag acgaaagggg taatttttat gtttagatat aatgtacgcg
     9421 gcgaaaatat cgaagtaact gaagcgattc gcgactacgt tgagaaaaaa gtaggcaaac
     9481 tagaacgcta tttcagtgac gcacccgatg caacggcgca tgtgaactta aaagtttata
     9541 cagaaaaaac agcaaaagtg gaagtaacga tcccattacc ttatcttgta ttacgtgccg
     9601 aagaaacttc tccagattta tacgcaagta tcgatttggt tgttgacaaa ttggaacgcc
     9661 aaatccgcaa attcaaaaca aaaattaatc gtaaatcccg tgaaacgggc atgaatactg
     9721 cagatgctac tgctttattc aatgaagagg acgaaaacga aaatgattct gaattagata
     9781 tcgtacgtac gaaacgcctt tcattgaaac caatggatag tgaagaagca gtcttacaaa
     9841 tgaacatgct gggacataac ttctttattt ttgaagatgc agaaacaaat ggtacaagta
     9901 tcgtttaccg ccgtaaagat ggaaaatacg gactgattga aacagactga tcagccttac
     9961 ttaaataaca acgaaaactt gcagcagcta ctgacagctg ccgcaagttt ttttgagttt
    10021 cctcctcttt tagatgaaat atgaataaat tatgaagttc ttactttaat acgcttaaga
    10081 tatttcaatt atctataact aatgataaaa tgaaaagtaa agtctacata gtctggataa
    10141 tacacagaag gaaaaggaga ttacacaacg catggccaat ttgattcgtt cacttatcga
    10201 aaacgataag aaagaactaa aacgtttaga tagcattgca aaaaaagttg aagcatacgc
    10261 tgaccaaatg gcagcattaa ctgatgaaca attacaggca aaaactgctg aattcaaggg
    10321 cagataccaa aaaggagaaa cattagatca attacttccg gaagcttttg cagtcgtccg
    10381 agaagcagca aaacgtgtat tggggttata tccacaccac gtacaattga tgggtggaat
    10441 cgtgcttcac gatggaaata tccctgaaat gcgtactggt gaaggtaaaa ccttgaccgc
    10501 aacgatgcca gtatatctaa atgctctgac tggcgaaggg gtacacgtag ttaccgtcaa
    10561 tgaatattta gcaacccgtg actcgacgga aatgggcgaa ctttataatt tcttaggatt
    10621 aacagtgggg ttgaatatca actcaaaatc ttcggatgaa aaacgcgagg cttataattg
    10681 tgatatcact tatagtacga ataatgaatt aggatttgac tacttgagag acaatatggt
    10741 cgtttaccgc catcaaatgg tacaacgtcc gctgaattat gccattgtcg atgaagttga
    10801 ctcaatcttg attgatgaag cgagaacgcc gctgatcatt tcagggcaag cagaaaaatc
    10861 cacagcttta tatacaagag cagacaactt cgtgaaacgt ttgaaagaag aagaagatta
    10921 taaaatcgat gtacaatcaa aaacaatcag tttaaccgaa gcagggatcg aaaaagcaga
    10981 agaaacattc ggtttggata acttatacga cattgaaaat actgcattga cccatcactt
    11041 ggatcaagca ttgagagcca actatatcat gcttttagat atcgattatg ttgtccaaga
    11101 cggaaaagta ctgattgttg accagttcac tggtcggatc atggatggac gccgttattc
    11161 agatggactt caccaagcaa tcgaagctaa agaaggcgta gagatcgaag acgaaacgaa
    11221 aaccatggcg acgatcactt tccagaacta tttccgtatg tacaagaaac ttgcgggaat
    11281 gactggtaca gctaaaacag aagaagaaga attcagagaa atttataaca tccaagtata
    11341 ccagattccg acaaatcgtc cggttatccg tgatgatcgt ccagatttgc tttacccaac
    11401 attacaaagc aagttccacg ctgtcgtgca agatatcaaa gatcgctacc gcaaaggtca
    11461 acctgtgtta gtcggaactg ttgcggttga aacatctgag ttattatcag atatgctgaa
    11521 tcaagaacgt attcctcatg aagtgctgaa tgcgaaaaat cactttaaag aagcagagat
    11581 catcatgaat gctggtcaaa aaggtgctgt gacgattgct accaacatgg ctggtcgggg
    11641 aacagatatc aagttaggac ttggggtaag agaacttggt ggtctagcag ttatcgggac
    11701 agaacgacat gaatctcgtc gtatcgataa ccagcttcga ggacgtgcag gacgtcaagg
    11761 agaccctggt atgtcacagt tctacttgtc attggaagat gatttgatga aacgattcgg
    11821 ttcagaacga atcaaatctt tcttagatcg gatgaagatc ggtgaggaag atgctgttat
    11881 ccaaagcaag atgctgacga aacaagtaga atctgcacaa aaacgtgtag aaggaaataa
    11941 ctatgacaca cgtaaaaacg tcttacaata cgatgatgtg atgcgtgaac aacgtgaagt
    12001 aatctataaa cagcgacaag aagtaatcat ggaagaaaaa agcttatcca agccactaat
    12061 gaacatggtc aaacggacaa tcagtcgtgt agtagatgcg catacacaat tagagaaaga
    12121 aaattggaat ttggaaagta tcatcgattt tgccggcagt actcttgtcc atgaagatag
    12181 tattactctt gcagatatcg aaggaaaaac agctcaggag atcaaagagt atctaaacga
    12241 acgagcacaa gaagtattta ataccaaagc ggctcaacta aatggtccag agcaactgtt
    12301 ggaatttgaa aaagtcgtga tcttgcgtgt tgtggactct aaatggacag accatattga
    12361 tgcaatggat caattacgtc agtcaatcgg cttacgtgct tatggacaga acaatccatt
    12421 agtcgaatat caaacagaag gctataagat gtttgaagat atggtaggcg ccatcgagta
    12481 tgaagtcact cgaatcttta tgaaagctga gatccgtcaa aacgtacagc gtgaacaggt
    12541 ttctcaagga acagcttctc actcagaaga cggagatgcc aaagaaaata gtaatactag
    12601 cgcgaaaaaa cagccaatcc atgtgaacaa ggtcggaaga aatgatcctt gtccttgtgg
    12661 aagcgggaaa aaatataaga actgtcatgg aaaaaatgca aactaatttc tgacagcagg
    12721 attggtgaga caaattcttt ttttaagaat tcgtctcacc ttctttctgt tatttgctga
    12781 ttctgttaga ctagatataa tgaaaaaagt atagtgaaag aaggaaaaaa catggaaata
    12841 tccgaaattc gaaatctcct gacagaaatg caggaaaagg tcacaagctt caggaggtct
    12901 ctttgactta gaccagctgg aagaagacat tgcggaagca gaagcaaaaa tggctgaacc
    12961 tggtttttgg gatgatagcg aagctgctca aaaagtgatc agtgaaaaca atgcaaataa
    13021 agaaacttat gatcaattcc attctcttgc tgaagaactg gaagaaatcg aattgatggt
    13081 ggagatgttg caggaagaac ctgatgcagc catgcaagaa gaagcagaaa cacgtatcaa
    13141 tgcgctagat gaaaaaatga agacatatga attatctatg ttgttagatg gtccttatga
    13201 tcgaaacaac gccatcgttg aattgcatcc aggggctggc ggaacggagt cccaagattg
    13261 gggcagtatg cttttacgta tgtatacacg ctgggcagag cagcatgggt atcaagtaga
    13321 gacactagat tatcaagcag gtgatgaagc aggaatcaag agtgtgaccc tactgatcaa
    13381 aggacacaac gcatttggtt atctgaaatc agaaaaaggt gttcatcgcc ttgtacgtat
    13441 ctcgcctttt gactcagcga aacgacgtca tacttctttt tgttcggtag atgtgatgcc
    13501 agagcttgat gaaacgatcg atatagaaat caatccagat gatttgaaaa tcgatacgta
    13561 ccgagcaagt ggtgcaggtg gtcagcacat caataaaaca gaatctgctg tacggatcac
    13621 tcacttgcct acaggaacag tagtagctag tcaggcacaa cgttcacaat tgaaaaaccg
    13681 tgagcaagcg atggggatgt taaaagctaa gctttatcaa ttagaagtgg aaaagaaaga
    13741 acaagaagca gctgcacttc gaggagaaca aaaagaaatc ggctggggtt cacagatccg
    13801 ttcttatgtt ttccatccat attctatggt aaaagaccat cggacaaacg tagagacagg
    13861 aaatgtccaa gcagttatgg acggtgacct tgatttattt attgacggct atttaaaaat
    13921 gcatctacaa taagaaccca ttgttaaatt gaaataaaat tgtaagattt tgcaagcatg
    13981 ttgaaacatt gccatgctat aatagctaaa gactgaaaaa gatatggaga gaacgccatg
    14041 attgaaatga aggatgtaat gaagaagtac tcaaatggta cgaccgctat ccgcaatctc
    14101 tcaattgtca tcgaacaagg agagtttgtc tatgtggttg gtccgtctgg agccggtaaa
    14161 tctacattca tcaaattgat gtatcgagaa gaaaaagctt caaaaggaac tttgaacgtc
    14221 gcaggtcatg atctaatagc aatcaaaaac cgcgaagttc cttatctacg cagagaaatc
    14281 ggcgtggtgt tccaagacta taaattatta ccaagaaaaa cagtatatga aaatgttgcg
    14341 tacgctatgc aagttatcgg cagaaaacct cgggatatca aaaaacgagt aatggaagtg
    14401 ctggaccttg ttggtttgaa acacaaagtt cgagtctttc ctagcgaatt atccggtggg
    14461 gaacagcaac gtgtctctat tgctcgggca atagtgaata cgcctaaagt acttatcgcg
    14521 gatgaaccga caggaaatct ggatccagaa aactcatggg aaatcatgaa attattagat
    14581 cggatcaatg cccaaggaac tactgtcgtg atggctacac ataacagtac gatcgtaaat
    14641 acgattcggc accgagtaat tgctatcgaa aacggccgca ttattcgaga tcaagcggaa
    14701 ggggaatatg gatacgatga ttagaacctt tttccgccat ttattagaaa gtatcaagag
    14761 tttgaaacgt aacggctgga tgacagttgc ttcagtcagt gcggtaacga tcacattgac
    14821 tttagtcggt gtattcatgg cagttatcat gaacgcaact aaattagctc aagatataga
    14881 aggaaacgtt gatgtctctg tattcgtaga tatcggtaca aaagacgaag agatgaaaaa
    14941 gctagaaaaa gaattgaatg acttagatca tgttaaaaaa gttgaatttt caagcaaaga
    15001 acaggaattg aaaaaaatcc aaaacgaaat gggtgattcc tggaaattgt ttgaaggtga
    15061 caataacccg ttatatgatg tgtatgtagt aagtgctact gatccttctt atacaaaaca
    15121 agtagcagaa gaagcagcag atttgaagaa tgtttctcgt tctgattacg gtggagcttc
    15181 ctcagatcgg attttccaaa tcgctggtgc agttcggaca tggggattag ttgcagcggc
    15241 attgctgctg ttcgtagcga tgtttctcat ttcgaacacg attcggatca cgattttgtc
    15301 tagacagcgt gaaattcaaa tcatgcgttt agtcggtgca aaaaatggat atattcgctg
    15361 gccgtttttc cttgaaggtg gctggatcgg tctgatcggt gcgattcttc cgattttgat
    15421 catcacattt ggttatgcat ggtttttcca attgatcaat ccatcattat tacgttccca
    15481 ttattcattg attcatccac aaaatatcgt ttggaaaatc aatcttttaa tggtcggcat
    15541 tggtgtgatc attggttcac ttggctcgat catttctatg agacgtttct tgaaattcta
    15601 ataccacaat ataaataaaa tgagaaagtt ggacagtttc gttccagctt tcttttttta
    15661 ttggctagga gaatagaagg aagcggcttt tttcccttta ctttaaaaaa caacttgggt
    15721 attcttaagt tggtagaaga agaggtgaag ccgtgaaaat tatcgttgac aattttttta
    15781 cgatcctttt gattatgaat attttgcttt cctttatcat cgtttttcga gaaagaaagg
    15841 aaactgctca aacatgggca tgggtgctcg ttttgatgtt cattccagtt gtaggattta
    15901 ttttatatat ttttttagga agagggattt ccaaagagaa aatctttgat ttgaagatcc
    15961 aagggaaaat cggtaagaac ctggaaattg aagaagacaa acaggcattg atgcgagggc
    16021 tttatcctca tccgcctact ggacaagtag atgtgaaaca attgatctat atgctcacgg
    16081 tatttgaaag tacgctctat acgactaaaa atgaaatcac tttatttaca gatggtcgtg
    16141 aaaaattcga tgcgctgatt gaagatatca agcaggcaga agaccatatc cacttccagt
    16201 attatattta tcgcagtgat gcattaggtg aagaagttcg agatgcattg acagaggcgg
    16261 caagacgagg ggtaaaagtc agagtccttc ttgatgcatg gggttccaca caagtttctt
    16321 ccagcttttt tcagaatttg aaaaaagcag gcggagaagt cgcatttttc tttcctctat
    16381 ttgttccgta tattaatcct cggatcaatt atcgcaatca tcgtaaaatc gtagttattg
    16441 atgggaaaat cggatataca ggtgggttca atgttggaaa tgaatatcta ggattggtaa
    16501 agaaatttgg ttattggcga gacaatcatt tgcgtattta cggagaagcg gtgtatacct
    16561 tgcaaaaccg ttttttgatg gactggaatt ctcagcatac aaaagaagta ggctattctc
    16621 ccaaattttt tccgtccatt cactctactg gtgatatagc tgttcaaatc gtgacaagcg
    16681 gaccagatac cgaacatgaa caaatcaaaa tgacttattt gaaaatgatt tcgttagcca
    16741 aacgggagat attgatccaa acgccttatt acataccgga tggatcgatt catgaagcac
    16801 tgaagttggc tcttctttca ggcgtaaaag ttcatatcca aatacctaac aaaccagatc
    16861 atcttctcgt ttactgggca acgtattctt tcgctgcaga gctaatcgag tatggagcaa
    16921 ggatcgaaac atatgagaat ggatttattc atgcaaagac gatgattatc gatggtggca
    16981 ttgtttcagt tggctcagca aatatcgatg ttcgttcatt tcgcttggac tttgaggtca
    17041 atacactggt ttatgatgaa cgaatggctt cacgtgtaag aaaagctttt tttgaagact
    17101 cgaaaatttc tacgcatctc acaaaagaaa tgtatgaaaa ccgaggaata atcatcaaga
    17161 tgaaagaagg attagcaaga ttgatttcgc cgctccttta aaacataaaa aggacatgag
    17221 aaccatcaac agatggagat ctcgtgtcct tttctaacgg ggccatttct gagagtacgt
    17281 cagaaatgga gaaagcgtct cgcatgagcg aaattaactt tggggtatct cttgtttttt
    17341 ggggaaagca agaaataagc taatatatgc tattaaaaga gacacatttc aaagccagta
    17401 tagcatagaa acttttaatg agatagtttt ttttacaatt atgagaattt attttgttat
    17461 ttaatgattt tgattcgttt tcatcaagta atgaaaatga taaaataaac atactttagg
    17521 attaaaggag atttctaatg aaaaaactta aattattctt accaataata ggattagcta
    17581 ttcttttatc aggctgttca aagtggattg atcgaggaga atccattacg gctgttggct
    17641 cttctgcttt acagccttta gtagaaacag ttgctgaaac ttaccagaca gaaaatcctg
    17701 gaaaattcat caatgtacaa gggggcggaa gcggaactgg tttgtctcaa gtacagtcag
    17761 gagcagtcga catcggaaac tctgatttgt ttgcggaaga aaaatcgggg atcgatgcct
    17821 cagcattagt cgatcatcga gtagcagttg ttgggatcac tcctattgtg aacaaggata
    17881 ttggcgtaaa agacatcagt atggaaaacc tgaaaaaact atttacaggc gagatcaaaa
    17941 actggagtga agtcggtgga aaagatcagc cagtagttat attgaaccgc gcttcaggaa
    18001 gcggaacccg tagcacgttt gaaaaatggg tattggacgg agaaacagct gttcaggcac
    18061 aagaacaaga ttcaagcgga atggtgcgcc agatcgttgc cgacacaccc ggtgcaatca
    18121 gctatgttgc tttttcttat gtttccgatg atgtagatac gttgaatgtt aacggtgtag
    18181 aaccgacaga tgaaaatgtg acaacgaaca aatggacgat ttgggcatat gagcatatgt
    18241 acacaagaga tcatccaaaa aaactgacaa aagaattttt agactatatc ttatctgatg
    18301 aagtacaaga taacatcgtt ggagaactag gttatatccc tgtttctcat atgaaagtcg
    18361 aacgtgattg gcaaggaaat atcgttaaag agtaatcttt acacaaaatt tacaaactta
    18421 gctaaacttt tacatgttat taaaaccaaa ttgatgttca atttgtacac tgagtgagta
    18481 tgttaaacct gattaagggg gagtcatctg tggaagatgt gaaaaaagta ttattaacaa
    18541 aatcaaaacg ttcaaaaatg gaacagcgtg gaaaattcat cagtttttta tgtatcgcgc
    18601 tgatcgtgtt ggttgtatta tctatttttt attttgtagc tagtaaagga ttggccacct
    18661 tttttactga taagatcaat gtatttgatt ttctgtttgg tacagattgg aatccaagcc
    18721 aaatcggagc aaatgggaaa ccaatggtag gggcattgcc tatgatcaca ggttcgttta
    18781 ttgtgacctt tttatctgct atcgttgcca cgccatttgc cattggtgca gcagtattca
    18841 tgacggagat ctctcctaaa aacggaacga agatcttaca gccggtcatc gagctgcttg
    18901 taggtattcc ttctgtcgtt tatggtttta tcggtttatc agttatcgtg ccttttgtca
    18961 gaagtatttt tggcgggact gggtttggta tcttagctgg tacatttgtg ttgttcgtta
    19021 tgatcttgcc gactgtcaca acgatgactg ttgatgcatt aaaggcagtg ccacggcact
    19081 atcgggaagc ttcattagcg cttggtgcca caagatggca aacaatctac aaagtcgtgc
    19141 tacgagcagc cgtgcccggg attttaacag cagtcgtttt tggtatggct cgtgcgtttg
    19201 gtgaagcctt agctatccaa atggtcatcg gtaatgcagc attgatgcca acaggtctta
    19261 ctactcctgc ttctacattg acgtccatct taacaatggg aatcggaaat acaatcatgg
    19321 gaacagttga aaataatgtg ttatggtcat tagcgctgat cttgctattg atgtcattat
    19381 tctttaatat cgtgatccgt atgattggga agaaaggaga gctgaaataa tgaacgcaaa
    19441 acgctcagat aaaattgcta caggtgtttt atatatagtt tcggggatca tcgtattgat
    19501 tttagcagct ttactacttt atatcttagt tcggggaata ccgcacattt catggtcctt
    19561 cttgaccgaa ccttccaaag cctaccaaga aggcggtgga atcgggatcc agctatttaa
    19621 ctcgatttat ttgcttttga tcacgatgat catcagtttt cctatttctt taggtgcggg
    19681 tatctatctt tcagaatatg ctggaaaaaa ctggctaaca gatgtgattc gcacgtcgat
    19741 tgaaatcttg agctcgttgc cttctgtcgt tgtcggttta ttcggtttct tggttttcgt
    19801 tattcaattc cagtatggtt tctcgattat ttctggtgca ttggctttga ctgtattcaa
    19861 cttgcctctt ttgactagaa cagttgagga gtctttacaa gcggtccatt acacacaacg
    19921 agaagcagga ttggccttag gtttgtcacg ctgggaaaca gtgataaaag tggtggttcc
    19981 agaagcgaca ccagggattt tgactggtgt gatcctaagt tctggaagaa tttttggtga
    20041 agcagctgca ttgatctata cggctggaca aagtgcacct gcgttagatt ttacgaactg
    20101 gaatccatta agtgtttcaa gcccgatcag cattttccgt caagctgaaa cattagctgt
    20161 ccatatttgg aagatcaata cagaaggcac gatgcctgat ggtgtttctg tttcggctgg
    20221 cgcttctgcc gtgttgatta ttgctgtttt actgttcaac ttcggcgctc gtgcgattgg
    20281 caaacgatta taccgaaaaa tgacttcagc ctaaggagaa gaagcaaaat gaaaaaatat
    20341 aactggaatg aaacaaatgt catcaccatg ccaaaagaga aagaagtagc actatatacg
    20401 gatgatctac atgtctggta tggcgacaat gaagcaatca aaggagtgga tctggaattt
    20461 gaaaaaaata agatcactgc actgattggt ccttccggct gcggaaaatc tacgtatctt
    20521 cgttcgttga atcgaatgaa tgatgggatc gctaatacga aaatcactgg gaaaatcatg
    20581 tatcaaggcg tagatattaa tgcgcctcaa gtggatgttt atgagatgcg caaacggatt
    20641 ggcatggttt ttcaacgtcc taatccgttc agcaagtcga tttatgacaa tattacattt
    20701 gctttgaaac agcacggcga aaaaaacaaa aagaaattag atgaaatcgt tgaaacaagc
    20761 ttgaaacaag ctgccttgtg ggaccaagta aaagacagct tggacaaaag tgccttggct
    20821 ttatctggcg ggcaacagca acgtttatgt attgctcgtg cgatcgcgat gaaacctgat
    20881 atcttgctgc tagacgagcc ggcaagtgct ttggacccta tttcgacagg aacggttgaa
    20941 gaaacacttg taaacttgaa agaagactat acgatcatca tcgtgacgca taacatgcaa
    21001 caagcagctc gtatcagcga ctatactgcc tttttctatt taggaaaagt aatggaatat
    21061 gacaaaacta acaagatatt taccagacca aaaattcagg caaccgaaga ttatgtgtcc
    21121 ggtcattttg gttaagacgg aggcctcaac atgacaaaag agattatttc atctaaagat
    21181 cttcatctgt attatggaaa gaaagaagca ctaaaaggga tcgatctaag cttcaatcaa
    21241 ggggaaatca ctgcgatgat cggtccttct ggttgcggaa aatccactta tctgcgttct
    21301 ttgaaccgga tgaatgacct gattccgaat gtaacgatca ccggaagtgt tttatataaa
    21361 gacaaagaca tctatggtcc aaaaacagat gttgttgaac taagaaaaga aatcggcatg
    21421 gtattccagc agccaaaccc atttcctttt tctatctatg aaaatgtcat ctatggattg
    21481 aaattaaaag gggaaaaaga taaaaaagtc cttgatcaag tagttgaaga aagcctgaag
    21541 gcagcgtctg tttgggatga tgtgaaagac aaattgcata aaagtgcttt atctttatcc
    21601 ggtgggcagc agcagcgtgt atgtatcgca cgtgttttgg cagttaatcc ggaaatcatc
    21661 ttattggatg aaccaacaag tgcgcttgat cctgtttcta ctgggaaaat cgaaagtatg
    21721 ctgttagaat taagagagcg gtatacgatg atcatcgtga cccataatat gtctcaagct
    21781 tcacgtattt cagataagac ggcattcttt ttagatggtc acttgatcga atacaatgat
    21841 acgaagaaga tattcatgaa tccagaaaaa caagagacag aagattatat ttcaggtcga
    21901 tttggataag gaggaaacgg aatgctacgt acacaatttg aagaagaatt gctgaatctc
    21961 cataatcagt tctacgaaat ggggatgatg gtgagcagcg ccgtgcacaa gtcagttcgt
    22021 gcatacatca aacacgataa aatcttagcc caagaagtta tcgaaaacga tataaatatc
    22081 aataacatgg aaacacgctt ggagaagaaa agttttgaaa tgatcgcatt gcaacagcca
    22141 gttacaactg atttgcgtat gatcattacg gtcatgaaag ctagctcgga tttagaacgt
    22201 atggccgatc atgcggtgtc tgttgctaaa tccacgattc gcgtaaaagg gcagacgaga
    22261 ataccggaaa tcgaaaaaga gatctccgat atgtccgatt atgtgaaaaa aatggtggac
    22321 aacgtcctag ttgcttatgt caaaacagac gaaaaagacg cacgtacgat cgccaatatg
    22381 gatcaaagag tcaatgacta tttcaatcga atctataatg caacaatcaa aaatatgcag
    22441 gaaaatcctg agacagtcat cagtggaaca gactacttga atgttgccag ttatctagaa
    22501 cggatcggag actatgtgac aaacatctgt gaatggatcg tctacttggc tacagggaaa
    22561 atcactgagt taaatacgaa ccacaatgag attttctaaa ctgaagctgt gattatgccg
    22621 tttgtttagg aaaagaggtt gtgaaatcag cggttgtgaa aaagctgttc tgcatcaagt
    22681 aataagaaca gtcaaacaaa aaatagctcc tcatattttt cgtttggctg gacttattac
    22741 cgcagatgca agcttttgaa caccgtttat tcgggaaagg gtttgtgaca aaacatgtca
    22801 caaacccttt tcatcatttc atagaaagga taaagaatgc aaaaaaaaat tagcatcatc
    22861 actatatttt ttatttgtgt gcttacttca tgggttattc agcatcaagc cgctttatat
    22921 gaagtacctg tcatcgagat agaaaaagta cgaataaaag aggatcatca attgattaca
    22981 ggaaaactga tgaacacgga tcaaaaagga gaatcgattt atttatcggt tccttatcaa
    23041 aaaaatcaaa tagaaacagc aaatataaaa gcgagacaaa tgtatctagt ccaaatgcat
    23101 ggagatgaat ggcagctagt agcaaaaaaa aatgatggct ggcttttctt cttcacaagt
    23161 atcttcattg gcgccatgtt gcttattggt ggaaaacagg gtctatttgc tttaggaagt
    23221 ctcatcatca atatcgtttt acttttactt ttgttgctca tctttactaa aacagaaggg
    23281 atttctctat taaatatatc tatcctgttc gtcgttatag ggatgattat tagcatattg
    23341 gctttagatg gatggaatag aagcagtata atcaaaattt cagctgcagt tacgagtgtt
    23401 tttttagcat tttttatttg tcttttgaca atgaatcatt ggaaagacca aggactgcgc
    23461 tatgaggaac tgaattatct gacacgaccg tatcgttctg tttttctttc tagcgtactg
    23521 attggaacag ctggaggaac actcgatacc gttatcacag tcattgcgac attagaagaa
    23581 ttgactaagc gacaaccaga aataacagcg aaacagttat ggctttcagg aaggcaagtg
    23641 ggtcgagatg tgattggctc gatgaccaat atccttttgt tcgtctatat cagtgggtca
    23701 gttccatcgc ttcttttata tttaggaaat caatggtcgt tcaaagaaac gatagaacag
    23761 catttatcat tggaattttt acgcgtcatt gctggcagct taggaatagt attatcgatc
    23821 cctatctcgg tcctcttttt tctgactgta aggaggctga aacgatgaat gtacttatta
    23881 tacttgttgg gattttagca atcagcgtgc tacttattgg cggtacgcaa ggtatgtata
    23941 ttcttttagg cttattcatc aatttaggga tctttttcct gttgttattc ggttatcatc
    24001 agaagtggcc gatactggca ttgtcggtca taggattttt attgattgct gtggttatct
    24061 tattttttat caacggttat aatctaaaaa tgcgtgcagc ttttgtgagt atactgatct
    24121 ttttattttg tttcctacta ttgcttccta tcactgattt tttagcaatc caaggtttta
    24181 catcggtaga actagaagaa ttatctggct tagataagac tttagcgatt gattttagat
    24241 tacttacgag atcgttgctt ctgattagtc ttagtggtgc tgttttagat gcgagtgtag
    24301 caatttctag tgggacattc gaagtatatc aggcaaatcc gcaattatct ttttaccaat
    24361 tgcgccattc tagtttcgct attgctaaaa aaatattggc gtcaacagta atgacacttc
    24421 tttttgcatt catgggaagt tctttagcgt tgatcctctg gtttattgat ttagacattc
    24481 cttttcagca agtgatcaat gaaaaatcat ttgttctaga atatacgatg gctattctta
    24541 ccacattgtc cgcactactt gttcttcctt taacctgcgt gactacagca tatctcttta
    24601 caaaaaagaa agaacaactc aagatggagt aaaagttaca acttaagtcc tatgacattt
    24661 ttactgaatc agtgcataat atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg
    24721 aggcaatttc catgaaagaa agagaacgta ttttagattt agtgaaaaaa ggaatccttt
    24781 cgactgaaga aggcttagat ttattagaaa gcatggcaac tgaaaaagac gaaaagcaaa
    24841 tcaaaaaaga agcagacaag gtaactgcta gtcataaaga aaaagatcag gcaagtcaat
    24901 tgatcgataa acttgaaaac ggcgaagaag aaatttctga gccagttgat ccaaaggaaa
    24961 gagaaagaca agatcaagaa aacttagaaa agatcttaga tgaactagca actgaagcga
    25021 ataaaacttc ggcgcgtcta gatgaagtaa atgctcaatt cgctgacaat aaggcagcac
    25081 gcaatgagaa acaggaagca ttgatgcagc tgaatacaaa agaagaatta ggcgagttga
    25141 cagaagaaga attagcacag cgtcagacgt tagaagcaga aatcaaagaa ttagaagcta
    25201 gtggcgatac actgttagaa gaacaattga aattagaagc agaattaaaa gatatccgta
    25261 agaatcaatg gtctgagaaa aaagaaacgt ttactgataa gtttgaattg ccagatgatt
    25321 ggaaagatca agcaacagat acactgaatc aagtaggcga aaaaatgtca gaagctggat
    25381 cacagttagg caaattcttg aagaaaacat tccaaactgt ttcagaaaca gtgaatgaca
    25441 acatggaatg gaaagatgtc agcttacgtg tccctgggat tgcgacaacg aaatttgagc
    25501 atgaatttta ctatgaagct cctgcagcaa gtatccttga tatcaaggca gcaaacggaa
    25561 acgtgacgtt aaaaacgtgg gacagcaacg atgtgaaagt tgaagctaag atcaaacttt
    25621 acggaaaaat gggtgctgaa ccatttgaag cattctctga acggagccag attgaggtta
    25681 atgaagatca tatctccttc caaattccaa ataaacgtgt gcgtgcagac cttgttttct
    25741 acttacctaa acgagtatac gaccacgcag ctattaaact tctaaatggc aacatcatga
    25801 ttgaaacatt ggaagctaaa gatatttata caaaatctac gaatggtaac atcatcgtgg
    25861 accaattaac tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa
    25921 acgggaacat cttagattct attatcgaaa cagtaaatgg tacagtgaca tttggtgcga
    25981 cagcagaaaa cctttctgtg tctttggtaa atggtgatgt acgtcttacg attaaagaag
    26041 ataatttgaa aaaagtagaa gcaagttcag tgaacggaaa tgtcaaagtc gctttaccag
    26101 atggcatcgg tttggaagga catgctaaaa caagtcttgg aagtatcaac agccgtctgt
    26161 ccaattacga agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg caattccgtc
    26221 gtgtttcaga tggtgaaatc gcgcaagtgc aactttcaac gacaacagga agtatctatt
    26281 taaaagatac agataaataa agaggttgtg aaagagatgt tcagccttga gcaataagaa
    26341 gcgattttgt aaaatagctt cccctatttt acaaaattgt gccttattgc cgatagtcta
    26401 tctcttgaac accgtttacc aagagattat gaacctgcca attatcgccg agtcacaagg
    26461 aataatttca gaaaaagctc gtcacgtttt atgaatatgg taatttgtga ccgagaattt
    26521 tgcagaaaga caccgattaa gaagaaggtt gtcacaacga ttttcttcat ttaaaatagt
    26581 agaacagagg tgagacatga tgaaaaaaaa gttgacaaaa tctaataaaa atgttgtttt
    26641 gacagggact ttagcaggaa taggagaata catcgggatt gatccgactg ttatccgtgt
    26701 gctttatgta ttcctgagct tagttgcttt tggaagtcca attattttat acattttatt
    26761 agctttgatt atcccatctg cgcgttctgc acgaacaaac tatggacata acaatgcata
    26821 ttatgaaaaa aacaattatc gtgaacaaac tcgtgaacaa acaaaaaaac gcaaagaagc
    26881 ggaaaaaatc gatgatgacg aatggagtga cttttaatgt cgtactttca acgtatttta
    26941 gttaatacgt taacattcat atcattggct gttctttttc ctgcgatggt ccatgtgagc
    27001 agtatttgga cggccgttct tgccagcttc gtattgtctg tgttgaatat gtttgtcaaa
    27061 ccgattttga cgatactgtc cctgcccttt acattagtca cattgggatt gtttacgttt
    27121 gttatcaatg cagcgattct taaaatgacc tccttttttg tgggagcaga acattttagt
    27181 ttttcttcat tcggtgcagc gttgttagta gctatcttga tgtcgcttgt caatgtaatt
    27241 gtcagcgaac ataacagtag aaaataaaag aaagctgtgc cgaactatcg gcgcagtttt
    27301 tttatttaga atcaggaatg cgttttctat tacattggta attaaataaa aacgtttttt
    27361 tta
//
LOCUS       DACDSY010000018        26823 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDSY010000018 DACDSY010000000
VERSION     DACDSY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.018"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..376
                     /locus_tag="IWK44_000691"
     CDS             <1..376
                     /locus_tag="IWK44_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tra protein"
                     /protein_id="HAP9549243.1"
                     /translation="INSNNKYACEYVLSFEREMRKMRAGVYFATQTPEEIIPKDSGSE
                     VADVIRNIFNLCTFKCFFNLDSALLEDIKKVLGNTITDTEIMLLPELEVGQAVVQTSS
                     EDTYMINFDPYPEQIERFDGGQ"
     gene            398..1534
                     /locus_tag="IWK44_000692"
     CDS             398..1534
                     /locus_tag="IWK44_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549244.1"
                     /translation="MEQNKNNNVASTLARQGMKQVSKKIGLALKIKIGVVIGLITLVV
                     LLVIGSLVTLQGTQTATNTGGVEVIGGNKPLSPEVKKWQKMVEKYAEKYGIKELVPYA
                     LAIMQCESGGNLPDLMQSSESAGLPVNTLQYEESIDQGLKHLGNVYNIAKSYGMESNY
                     DGIVQAYNYGIAYMHYLGKNKKPHTIETAEAYSKNVVAPSLGNTTGQTYSYVNEVSIK
                     YGKTYLYLNGGNFYYAELVKQYIGGGSGNPANPSEFWGKMKTEMEKYQGWPYVWGGKN
                     PTTGFDCSGLTAYLYQFSAGITIESYTVTQYEATEAVSIKEAKAGDLIFFRGTYGAPD
                     FISHVGIYVNETTMYDSNSSGIGYHNWQDSYWQSHSPEIRRVKK"
     gene            1548..2198
                     /locus_tag="IWK44_000693"
     CDS             1548..2198
                     /locus_tag="IWK44_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP9549245.1"
                     /translation="MIKRKNDNKWKYICGVLLVILVLGGLLSQLHTSTIQAKNKKLDQ
                     EVTQLEEQNSKLSKNINDLNANIEANLTNDDGSELNKQIAEVAKKFVELYPVYDIEKV
                     EEKKAELEKVSTTSVADSIVPDDMITTSKKTLESKSTTQKGEAYSSDPTFKSKYKDSK
                     LYTNYVNANTINYFAEVNYKTESSSGDTENTVYMLFEVTNKDGRVLVDNYEIKYLK"
     gene            2212..3108
                     /locus_tag="IWK44_000694"
     CDS             2212..3108
                     /locus_tag="IWK44_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549246.1"
                     /translation="MNKKYSIHNKKETNKKNKSQIDKVYKKTKGMKLYLGAILGFLAV
                     GMMIVFSPYVMGKSYDFETVKMNSAQNVTSSLNMYIDDMEINREDGLFKLVLRYQDDT
                     GTKSLSNIKSDYRLNFITNKNNEGVETKIIQLSDEYTVIYYQNLPKNFGVISVTVHPR
                     YIYPELEPSNDLKEKEIKFYAVDKDIKSNNKLEVESTSDLQKDNFKYQIKAIEKSIKD
                     EKKSIEKMNLANEVVQKDIDKANQNLEFQTLDEQTDTKNEISSMQTTIENNKSSIEES
                     ENKIKQLEEKIKQLEKSASRFE"
     gene            3144..3374
                     /locus_tag="IWK44_000695"
     CDS             3144..3374
                     /locus_tag="IWK44_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549247.1"
                     /translation="MCNSIVTIKEKAEEELMRIEAEYKEKRKAIKRKKNAELKKYKEE
                     QALKLGMMLCKYYDCYDINEVIKKANLNQICE"
     gene            3726..4022
                     /locus_tag="IWK44_000696"
     CDS             3726..4022
                     /locus_tag="IWK44_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549248.1"
                     /translation="MDVSIRGIDPQYVKEIDKKVNKIQERTGKKFSRNDYFLAIIRQD
                     SEIDLIDYKKSEFDLVVEKLLLSNEQMVESISELTSVYERLFNFLISERSDLNK"
     gene            4019..4486
                     /locus_tag="IWK44_000697"
     CDS             4019..4486
                     /locus_tag="IWK44_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549249.1"
                     /translation="MNYPYFKVSATEDVKEKFNKCFEVNKSVFQTKSNMFRYVVSNLP
                     MLTKANQNFRDPEKEELAKNLEEAKLKISELETIIELQKQQLENFNKKEDSTHKDYSE
                     QLNSIEESVYKLLINSGLASKELEEERAEPEQESSRLGEGTMFSSPSLRFTTD"
     gene            4553..6088
                     /locus_tag="IWK44_000698"
     CDS             4553..6088
                     /locus_tag="IWK44_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549250.1"
                     /translation="MVGDDMTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDY
                     ISRQEAIRQDKLDHDYSEDELNELARIEKALEKIEQETDQPVIKDLREMDKYIDYMTR
                     KKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNGSVMFQDVISFDNDFL
                     VREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHVTAM
                     ERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLEL
                     KQSVKDSLIQLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNY
                     MTKDNPKRKEYDRMTKEIDELYQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLL
                     KQSEEKNLKSNELKNLLDGKKLNVKFDREKPEFKTKYAKQSYDDFQKRLEERQLAREK
                     NEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMIEQEKMRQAREFEI
                     GMN"
     gene            6110..6439
                     /locus_tag="IWK44_000699"
     CDS             6110..6439
                     /locus_tag="IWK44_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549251.1"
                     /translation="MKNFDIVCSNTKNIYLRELLNSDSETIEDVKKIIVLFEKENMEL
                     ENWGLFEIPISGNYCFYNWKTEDDVAFANYFFDKNYFSPLYIDKHSNEQVASSIKEAI
                     KLERVRK"
     gene            6441..6704
                     /locus_tag="IWK44_000700"
     CDS             6441..6704
                     /locus_tag="IWK44_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549252.1"
                     /translation="MIDFRTVMDKLSNGDFRVLIELYLNVTSTVKEFLYSNPKTALVL
                     VAVLIVFAVISFIKKMIPLLIIGAVLILGLYFPEVLDQIKNIF"
     gene            6879..7745
                     /locus_tag="IWK44_000701"
     CDS             6879..7745
                     /locus_tag="IWK44_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549253.1"
                     /translation="MKKLRVIATIPMIFSFALLLSACGKEEAGAKSNEAHNISFIDSA
                     EQKDSRCWLVVETSSKNKTPIDKNEYVKAVIQTKDGKAKVFKTDMEQLTLEKVSKMSD
                     EEIIEQALNADKQKFNDKKKSDIDSYNATLDSVKTELENEEKQEFQNDQKIADFQDSI
                     PKIQADIEEIDGVQYSEPEWKMVSATIKMDNENKSPSMETVKLSYKYLDSPIWNLNSF
                     GEVSSEGELSYESTSMDLKLSIPVATTKVLDNFYSGYSNDELDQLLLTKVASKDNKVQ
                     LDSKGKNVSIVE"
     gene            7900..9726
                     /locus_tag="IWK44_000702"
     CDS             7900..9726
                     /locus_tag="IWK44_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9549254.1"
                     /translation="MAYKKGNWKPKTKEQKQKELDDLIKASNSKIEQYQSNPEDMIEF
                     AKFMSKIHNYSPLNLSLIDEGFEGAIAVASYDGWKKLGFQVKAKEKGIGIYAHAPVTV
                     FIDENGEEKTLKEATNEQKEKIKNGELRSKKIKHFKKGYVFDISQTNATEADLPKIFP
                     NRVWNFNIDGELSVSQLEKGVNHLAESMNISIKDMNESGRGEMGSARGEYIQYVDGRE
                     EIVLNSRNSRTQNLTTSIHELAHKKLHNKNEHGSSYSTPIKEFQAEMTSFIVCHNYGI
                     DTSDFTIPYIANWTKNNEKIDPKNFRQIMQEVRETSIDFISTIDKKVLEEQQTAFNAL
                     KNSFVPNENKEQIQQKSDLEFHNFYDSTINDPDRTIHGSYLEAMVDYYRGKYGENSIE
                     LTHSNYDVTAKIGENYVTYYSDTKQFDVCDVYGVVFENPSENKHDRLLLDRSPENCDY
                     VEKFVVESLKACENHELKNDTLKLSEPLRSVVINEAVYNLSENANNYYNEKCWQEEIE
                     NQTNINDVKELNSCMTAEDFYESHKQEIENTITDFESVSGTNLRDTIDNESYKEVASV
                     VAYKLVATKVKYEMDEGTFKIPENSNLEKPTVKTAERSFEME"
     gene            9735..10808
                     /locus_tag="IWK44_000703"
     CDS             9735..10808
                     /locus_tag="IWK44_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549255.1"
                     /translation="MSNEQNYGTGGTYRLFHGSMNKFDEFSLDHKGLNGTANGFGVYL
                     TPNKEMATMYAEQGSNGNNGFVYEVNVELEKPISMNERTITDEELSKVIDKLQESNDI
                     LNDYNDVSYYGEKVVKREVIASLSENENDVDLINDIGNTIGDHEATAKAFYDVGKYTH
                     IIAEEQLRQKDVVIVVLQPDKVKIETIHDLNEERNSLESSEDMTDKFVGHKVSEVPIR
                     EVVIKDFEQELETTNYYDKQAWQERIEYLENMEEPVSRLALDSDVNEFYDSHKEEIET
                     KLTNLSEKLEIDKEELLGFPVDTSSTEYKKATSIMAYNGAMREIYDEIENDTFEIPPV
                     SEEMENKILKLTNFENTTKFEME"
     gene            10817..11077
                     /locus_tag="IWK44_000704"
     CDS             10817..11077
                     /locus_tag="IWK44_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549256.1"
                     /translation="MIGQENRNFLETQGEYRQINNSHFVICEDVQIAFKMALKYGKSI
                     RKKIIREKKSKKIGFWELSLRDGLNFIFCTEDYYEKYKCTLS"
     gene            11114..12118
                     /locus_tag="IWK44_000705"
     CDS             11114..12118
                     /locus_tag="IWK44_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAP9549257.1"
                     /translation="MDLKKAKKVSIVDFCKQNGIGLKQDSREYYRLEDHDSCVISDRK
                     NLFKWNSKDLGGDIFDFIKAYYDCSFKEAKQKLEDSDYEVHSFNTKVKKSPYQYQKEN
                     EVPVSVAKSYLVNERRIDIALVEDFLSKGLIKQDKKGNVLFLWKKDQEIVGCTEQGTR
                     KFFHEGKQKDVTWKKIQENSKEYSGFNVTFGQPDKMYFFESEIDLMSYITQKPEKAEN
                     ATFVSMNGLKVGNVLHGVTEHYNKFKSLPSETVLCVDNDKAGRTFIETEFKEKGIGRE
                     GIGISEVKVDVPLREGFDWNDNLTRGKEAFKRFKLEDVSKIVNFTHKLSLNTPVYEME
                     "
     gene            12143..12499
                     /locus_tag="IWK44_000706"
     CDS             12143..12499
                     /locus_tag="IWK44_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549258.1"
                     /translation="MNDTIAILSDSLIDLDKTSTDYMIIEEQLKNKNPEFDIQEKSLR
                     IELPKFKQKKLITILFEIIERYGLTYYQIWTDKKDGQYTKGVSIEAVREDDQVSIKAV
                     QPLMGYNEELLNCLNV"
     gene            12661..12852
                     /locus_tag="IWK44_000707"
     CDS             12661..12852
                     /locus_tag="IWK44_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549259.1"
                     /translation="MEQTRMFWSDRMQDHLDCIYKTREQLNKLDTVRYYFTYFSENDE
                     QRWDIFHDGNYEEFFYTES"
     gene            12961..13179
                     /locus_tag="IWK44_000708"
     CDS             12961..13179
                     /locus_tag="IWK44_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549260.1"
                     /translation="MVNKKETFKVGIEVQGVVIGSGELIEGEVISLLANVAVVKAIGK
                     APVVMKKCSMMCLDGLNNPITQRFENVI"
     gene            13216..13533
                     /locus_tag="IWK44_000709"
     CDS             13216..13533
                     /locus_tag="IWK44_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549261.1"
                     /translation="MKDYASRKFIHLEPMKELVINGNEYNDYKSLFVKEQPKGGIRMK
                     NLPDFRLIEVNNLGIIEDYLAKLDEAVKQSIEKNGEDHVLTVVLKDLKDRLEAEAENF
                     NKD"
     gene            13544..13741
                     /locus_tag="IWK44_000710"
     CDS             13544..13741
                     /locus_tag="IWK44_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549262.1"
                     /translation="MRDMTEAVKELKKMYPDVLNMTVDDFHEALKNAESEEERTFYLT
                     LSSFVTRVDQKKVINQKDFKI"
     gene            13738..14706
                     /locus_tag="IWK44_000711"
     CDS             13738..14706
                     /locus_tag="IWK44_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9549263.1"
                     /translation="MSLGNEKPYYVVIGGVNGAGKSTIYQIQNLKRKELDTDRINADE
                     ILRSFGGNWKKQADINKSMREVIREIKDHFKEGKSFHHETAFVGNEYPHKKRLLNAKK
                     LGYESFLIYVGLDSPEKAIERVKSRVAKGGHGVGEDLIRERYVSSLRNLAGLEKYFDN
                     VSIYDNSDEFSYIYVKENHTVVANNTQEYNWVPKAIQEKRAEIPISKQIIKDISQEGL
                     SPKETLERIYSESDDFISDRFFKAEQVDDFYMKHKNEIKERVLAISFSTGVSEDELVG
                     QDKKRNSCKLAYINSVGELLEKTDSELSSMEASRDRSIKKTDFEIE"
     gene            15205..15768
                     /locus_tag="IWK44_000712"
     CDS             15205..15768
                     /locus_tag="IWK44_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9549264.1"
                     /translation="MKFGGHFLNKKQDQEYYIEKLEKENLELKERIRYYESKFHKRSD
                     CMKPNLIETGKRIKSIRSNLGMTMEQFAILTDSSNTSAVNNWERGYNLPNKTKLKKIA
                     ILGNTTTDWIKWGTLEEYITSYLIGIGYELYIKDFPEIPHKVFKDIQEKYSDTFSLDK
                     DYELLDSIIKNIFTKYYSKEFEVNQIP"
     gene            15782..16564
                     /locus_tag="IWK44_000713"
     CDS             15782..16564
                     /locus_tag="IWK44_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9549265.1"
                     /translation="MKPNPVETGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYIASYLKDSGYELYIKDFPEIPHKVFND
                     IQERYSNTFSLNKDYELLNPIIKNIFTKYYSKDFEDYRDIEVKPDPKKVGRKIRSIRK
                     KLGLTMQEFGCEVSNSPRSTVSTWEHGGNLPNKAKLKKIADIANCSVEDLLFDEGFIS
                     EKSQVKLINELKEENSKLKEKIKYYECLFDGIESLLNSRKNS"
     gene            16604..17215
                     /locus_tag="IWK44_000714"
     CDS             16604..17215
                     /locus_tag="IWK44_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9549266.1"
                     /translation="MLVGYARVSSDDQNLERQLEEFRKIGVEKVFAEKKSGRNTVERP
                     EFNKMLAFVREGDTLVFESLERLGRNSDEILDTVSFIDKNGMGLMVLNLPILDTKFGD
                     PNLEKLVRSLVINIFSWTAQNEREEIKRKQKQGIAIAKKKGVYKGKPYEYSSTAKNPQ
                     KRFIYNEIVRMLGEGVPIKRIAEKTGTNRNLVYRIKKRISESD"
     gene            17454..17642
                     /locus_tag="IWK44_000715"
     CDS             17454..17642
                     /locus_tag="IWK44_000715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549267.1"
                     /translation="MAYETSEGKEIKTVEIEVGKVQQLFDNFAQKLPESLLKELDDLE
                     YEIIGMARVISVRDSNEQ"
     gene            17743..17931
                     /locus_tag="IWK44_000716"
     CDS             17743..17931
                     /locus_tag="IWK44_000716"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549268.1"
                     /translation="MEILYGILRAIAVILGEIIESLTGLINSIFGTMLGKLGLVILLI
                     VAMCLDNYFRKKERRINE"
     gene            17924..18175
                     /locus_tag="IWK44_000717"
     CDS             17924..18175
                     /locus_tag="IWK44_000717"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549269.1"
                     /translation="MSKCANGRDNGIGGRIQMQLKNRIEVETPYYFKGSSCLSLFIEQ
                     VEGEHDQYNLSSEYERLFTGIPFGEVINEYKKIKSDFYA"
     gene            18216..18407
                     /locus_tag="IWK44_000718"
     CDS             18216..18407
                     /locus_tag="IWK44_000718"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM--benzoic acid carboxyl methyltransferase"
                     /protein_id="HAP9549270.1"
                     /translation="MPPEDYQPPFCFECDGEILKSGNLMVCEECGKVYTHEEYQKLTD
                     EEAEKYIDFMKKKSPELFG"
     gene            18437..18640
                     /locus_tag="IWK44_000719"
     CDS             18437..18640
                     /locus_tag="IWK44_000719"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549271.1"
                     /translation="MPQIRVECRYCDNPCKPRNVDGDLVCSNCGAEWASAKCEIKVSD
                     RELERECKEQAEFDQWMAQYGED"
     gene            19137..19700
                     /locus_tag="IWK44_000720"
     CDS             19137..19700
                     /locus_tag="IWK44_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549272.1"
                     /translation="MTAIYKALKNSNVSEYVKSRVLNNNLDYILENGVADLISKLSDP
                     IDIDHLVVWQDGTLEVSIYGKFYSRNKVTTFKNSISGREDLKGINKNSKFYRTNKELL
                     EKISKCVSQLVNPFSKKRSKELFGIFYDGFNAKSNVEKFFYFNDSAYIAVKDSKRIPE
                     ATLEYLEPIKLSVFYSLYEKEIANEAI"
     gene            19687..20571
                     /locus_tag="IWK44_000721"
     CDS             19687..20571
                     /locus_tag="IWK44_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HAP9549273.1"
                     /translation="MKRFKGSRVSKKVGKELLSLGLKKEIVKFRNMYREPEFRLIFQL
                     DIVRGLKISFSCLGYLVWLFIARVGWSNAYSFYKLFSGTDRLKESLEVKEALNNLGSI
                     AFSLFIFALFILQVGKIMMSTNDNYIDYLKPFQKGIKTICRQGTVLFLLGLYEHEVHF
                     QNYYNTTAKLGSFLLLYLVSNIAEKILFKYIVKQVFSDIQKESYCLKTPYGNLKNVDL
                     FLFKIDSKWDKYIYRSKEVFLTGSYNEILQVSLYDTGAIFNNEPMVLKDFFGDYELIW
                     NPKQFSEQGAENEFAEVP"
     gene            21069..21323
                     /locus_tag="IWK44_000722"
     CDS             21069..21323
                     /locus_tag="IWK44_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549274.1"
                     /translation="MYNVGDQIDEVVKIGNVKLDKSKYYISETEITFNTESGCTLIWR
                     LPFPKDEATLKKINTLKIRYCIYEVIDKNTYVITEVKVIG"
     gene            21722..23323
                     /locus_tag="IWK44_000723"
     CDS             21722..23323
                     /locus_tag="IWK44_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9549275.1"
                     /translation="MLNSCEYADILAKILKNGIKQFDSKNLVAFKKNSNKIGAIAGFT
                     SKNNMIKTKGLIYRSIDRVLADSDVLTHWTPNVYSWLGGGENKTIFGHKEANLIQINT
                     FVADIDYPIGEKKTDLNLLVLYLLEEGLLPTLIVDTPKGYHCYFMIQNYNQENDSFDK
                     ASYISNTNDFKSLRVAKRISENIRKSIKNRLPQVDMGCNHFGIFRFPTKKNIVHYEPN
                     FVDTFEGYLKWSKDFEKKEYALKRSNLSLLAPNYTGKKGSTRQIDTKWYDFLIHTDIA
                     EGDRNKTIFTLALACKSSGLSIGECMDQLDQVQFNNDLSYSEVERTIRSAYRGDYKGA
                     KSSYVNEIIENYATPTEFEKYSINVNGQAVKRKGNRVDPSNWVKFAKPREERKYSHFE
                     ESKADLLAYLERKQRSLKKNELLLTISMATISKETLIPVSTLKVILKELRTANTVILQ
                     TSRGRYGSTKIATSKFVEAKILNAVIQNKKDKQVFVLELLGTANKHVETVLRRYEDKT
                     VPFHTNILKEREKSKHAPIRGANTG"
     gene            23545..23844
                     /locus_tag="IWK44_000724"
     CDS             23545..23844
                     /locus_tag="IWK44_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9549276.1"
                     /translation="MSVKKYVYSHPINVFIIGKLGLSVEVFCELYGFKQGTLSSWITR
                     DREIDDFPVSFLYALSLASGENMNEVYDCLSEYEKEYKEYLKVSAPKRKKKYIEE"
     gene            23977..24351
                     /locus_tag="IWK44_000725"
     CDS             23977..24351
                     /locus_tag="IWK44_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAP9549277.1"
                     /translation="MNIDGIYNESDFKKGMVDIDRKEYLFPDEVGFFKIEGETVCRYW
                     GKKGNIILLVHTVEGRKLKFSFWSNTKYCCEPVPKRKIDAAKESVRFLPDRSKITLIC
                     ERSSNAKFTRIRDILFETTSSQ"
     gene            24400..26763
                     /locus_tag="IWK44_000726"
     CDS             24400..26763
                     /locus_tag="IWK44_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAP9549278.1"
                     /translation="MAVVILAEKPDQGAKFASALAGKKLSKKSGKYEFKSEIFGDTIV
                     TWGIGHLVGLSLPEKYEWLPNKDKWHLDNLPFLPKFNELKYEVSKGKNQQFSIVKSCL
                     ENADEIIIATDPDREGENIAYNIFKLCKQSIFNKPIKRLWINSLVQKEIIKGFKNLRN
                     SEETISYFKEANARQIADYLVGMNYTELYSLKLQEKGISGVVSLGRVQTPVNTLVVEN
                     DLAIRNFRPEKYKTIECHTTDKQPKAIFKNSLEYFKLEKFEEDTKKFGLDSASTGIIK
                     KIEVSKKQTEPQKLFSLGGIQEYANKKWKYSAKKTDKIIQSLYDRGYLSYPRTDSELI
                     TTNEFEYLKANLDVYKSVLGISIDTPCLEPKKRFVDNDKVLEHYALIPTMDIPDIANL
                     TLDEKNIYEIVTRRACLMFAEPYKYENTKVTLDVNGLEFTTSGNVPISLGWKQVDTNL
                     DESDDKEETLPKFHEQETINIDIRFIDKLTKEPKRLTEGKLLGKTGIMAKLGLGTPAT
                     RSNIVDTLLNREYIKIENTNVYPTPKGYLLWDLTKNKDLLIGKPENTAIWEESLGKIA
                     QGNYTQEAFLRNIYNYINMTVSDLKKIEFTSDYLEKTVNQNKFDIGNYQVEEKNKVFS
                     ILNNTSGEKFIIFKRISGKAINKKILQELLQNNRTDKVLSGFKSKEGKAFNARLLFDP
                     NVMKVTMEFEKNSPNGDKKSIHDIVNGYEVVEKTKVFEIKELATGDIFIFYKNNSGKT
                     MSLKMVKELLENGKTKEKITGFISKDNKKYSAFLIFDSKNKIIKKEF"
BASE COUNT        10649 a         3163 c         5112 g         7899 t
ORIGIN      
        1 tattaattca aataacaagt atgcatgcga atatgtacta agttttgagc gagaaatgcg
       61 gaaaatgcgt gcgggagttt actttgcaac tcaaactcct gaagaaatta ttccaaaaga
      121 ttccggatca gaagtagcag atgtgattag aaacattttt aatctatgta cttttaaatg
      181 cttctttaat ttggactctg cattgttgga agacattaaa aaagtgttag gaaatacaat
      241 tactgataca gaaatcatgc ttttaccaga attagaagta ggacaagctg tagttcaaac
      301 ttccagtgaa gacacctata tgattaattt tgatccatat ccagagcaaa ttgaacgatt
      361 cgatggtggt caataataga gatcggaggt gaatttaatg gagcagaaca aaaataataa
      421 tgttgcttca acactcgctc gtcaagggat gaagcaagta tcaaagaaaa taggtcttgc
      481 tttaaaaata aaaatagggg tagttatagg gttaataaca ttggttgttc ttttagttat
      541 aggttctttg gttacattac aaggaacaca aactgcaact aatacaggag gtgtagaagt
      601 cattggtggc aacaaaccgt tgtcacctga agttaaaaaa tggcaaaaaa tggtagaaaa
      661 atatgctgaa aaatatggga ttaaagaatt agtaccttac gctttagcaa ttatgcaatg
      721 tgaatctgga ggtaatttac cagatttaat gcaaagtagt gaatcagcag gattgcctgt
      781 aaacacactt caatatgaag aaagtatcga tcaagggctt aaacatttgg gtaatgtata
      841 taacattgcc aaaagttatg gcatggaaag taactatgat ggaatagtcc aagcctacaa
      901 ttacggtata gcttacatgc attacttagg aaaaaacaag aaaccacata ctatcgaaac
      961 ggctgaagcc tattctaaaa atgttgttgc tccaagttta ggaaatacca caggacagac
     1021 ttattcatat gtaaatgaag tatcaatcaa atatggaaaa acatatttgt atttaaatgg
     1081 tgggaatttt tactatgcag agttagttaa acagtatatt ggtgggggta gtggtaatcc
     1141 ggccaaccct tcagaatttt gggggaaaat gaaaacagaa atggaaaaat atcagggttg
     1201 gccatatgtt tggggcggta aaaatccaac aacaggtttt gattgtagcg gattgacagc
     1261 atatctttat caatttagcg ccggaattac gatagaaagt tatacagtaa cccagtatga
     1321 agcaactgaa gcagtatcaa ttaaagaagc aaaagcaggt gatctgattt ttttcagagg
     1381 aacatacgga gcgcctgatt tcatttctca cgtaggtatc tatgttaatg aaacaactat
     1441 gtatgatagt aattcaagcg gaattggtta tcacaattgg caagattcat actggcaatc
     1501 acacagtcca gagattcgta gagttaaaaa atagggggaa gaaaaagatg attaagagaa
     1561 aaaatgataa taaatggaaa tatatttgtg gtgttttact tgttatttta gttttaggcg
     1621 gactgttgtc tcaacttcat acaagcacca ttcaagcaaa aaataaaaaa ttggatcaag
     1681 aagtaacaca attagaagaa caaaatagca agttaagtaa aaatattaat gatctaaatg
     1741 caaatataga agcgaatcta actaatgatg atggtagtga attaaataaa caaattgctg
     1801 aagttgctaa gaaatttgtt gaactatatc cggtatatga tattgaaaaa gtagaagaaa
     1861 agaaggcaga attagagaaa gtatctacta cttctgtagc tgattctatt gtgccggacg
     1921 acatgattac aacgagtaaa aagactttag aaagtaaatc tactactcaa aaaggggaag
     1981 catattcaag tgatccaaca ttcaaaagta aatataaaga ttcaaaatta tatacgaatt
     2041 atgttaatgc caatactatt aattactttg ctgaagtcaa ttacaagaca gaatcttcta
     2101 gtggtgatac agagaataca gtttatatgc tctttgaagt aactaataag gacggccgag
     2161 tgttggtaga taactatgaa atcaagtatt taaaatagtg aggtgaagta aatgaataag
     2221 aaatattcca ttcataataa gaaagaaaca aataaaaaaa ataagtcgca aattgacaaa
     2281 gtatataaaa aaacgaaagg aatgaaatta tatttaggtg ctatacttgg attcttagca
     2341 gtaggaatga tgatcgtttt ttctccttat gtgatgggaa aaagttatga ttttgaaact
     2401 gtaaaaatga attcagccca aaatgtaacg tcttctttga atatgtatat cgatgatatg
     2461 gaaataaata gggaagatgg actgtttaaa ttagtgttgc ggtatcaaga tgatacagga
     2521 actaaatcac taagtaatat taaaagtgac tatagactaa atttcataac gaacaaaaat
     2581 aatgaaggtg tggagacaaa aattatacaa ttgtcagatg aatacacggt aatatattat
     2641 caaaatctac ctaaaaactt tggtgtaatt tctgtgacag ttcaccctag atatatttat
     2701 ccggagttag agccttcaaa cgacttaaaa gaaaaggaaa taaaattcta tgcagtagat
     2761 aaagacatta aatcgaataa taaattggaa gttgagagta cttctgattt acaaaaagat
     2821 aattttaaat atcaaattaa agctatagaa aaatctataa aagatgagaa aaaatctatt
     2881 gaaaaaatga atctagcaaa tgaagtagtg caaaaagata ttgataaagc taatcaaaac
     2941 ttagagtttc aaaccctaga tgaacagaca gatacaaaaa atgaaatcag ttctatgcag
     3001 acaactattg aaaataataa atcttcaata gaggaaagcg aaaataaaat caaacaactt
     3061 gaggaaaaaa taaaacaact agaaaagtct gcaagccggt ttgagtgatc gtgtttaaat
     3121 tattttaagg gagtttagag aaaatgtgta attctattgt cactattaaa gagaaagctg
     3181 aagaagaatt aatgagaatt gaagcggaat ataaagaaaa aagaaaagca attaagcgaa
     3241 aaaaaaatgc agagcttaaa aaatataaag aggaacaagc gttaaaatta ggaatgatgt
     3301 tgtgcaaata ttatgattgc tatgatataa acgaagtaat aaaaaaagcg aatttaaacc
     3361 aaatttgtga ataggaaaaa gagtggtaag gtaaaacttg ccactctttt tttgtgcgca
     3421 aaagctttag tttctatgac caaaactacg gacgaaacta tgaccaaaag taggtactat
     3481 attatggccg aaaataggtc gaaaatagat atttttgtaa tagcaaaaaa atatgaggtg
     3541 attttgaaag aaaaccagtt ggcacagcca acttagccac cttcctttgg gaaggtgcta
     3601 tcagttttcc cttatgctct ttgtattaat tccttggtct tgtctttaaa tctttaattt
     3661 tcctttggac tttgatataa taatatatac cagatagata aaaacagctt gaaagaggtg
     3721 tgtgtatgga tgtttcaatc agaggaattg atccacaata tgtaaaagag attgataaaa
     3781 aagttaataa aattcaagaa cgtaccggaa aaaagtttag taggaatgac tattttttag
     3841 caatcattcg ccaagatagc gagatcgatt tgattgacta taaaaagtca gaatttgatt
     3901 tagtagttga aaaactttta ttgtcaaatg aacaaatggt agaaagtatt agcgagttaa
     3961 cttcagttta tgaacgctta ttcaattttt taattagcga aaggagtgat ttaaataaat
     4021 gaactaccct tatttcaaag tatcagcaac agaagatgta aaagaaaaat tcaataagtg
     4081 ctttgaagta aacaagtcag tatttcaaac aaaatccaat atgttccgtt atgttgtttc
     4141 aaatttacca atgttaacaa aagccaatca aaatttccgt gatccagaaa aagaagagtt
     4201 agctaaaaac ttagaagaag caaaattaaa aattagcgaa cttgaaacga taatagaatt
     4261 gcaaaaacag caacttgaaa actttaataa aaaagaagac agtacacata aagattatag
     4321 tgaacaatta aatagtatag aagagtctgt ctataaacta ttaattaata gtggattagc
     4381 tagtaaagaa ttagaagaag agcgtgctga accagaacaa gaaagtagtc gtttgggaga
     4441 gggaacaatg tttagttcac cgtcattaag atttacaacg gattgaacac taaaaaattc
     4501 taatgcaact tagcattaag tacaaaaccg cctgtagaaa aaatttttga aaatggtggg
     4561 tgatgatatg actgaacata tctttaacag aaaaagtgcg agcattattc ttaaagcaaa
     4621 atttgaaact ggaaaaagca aaaagaataa tcacatggta gaacttcaaa aatttgtaga
     4681 ttatatttcc agacaagagg ctattaggca agataaacta gatcatgatt attcagaaga
     4741 tgaactaaat gaattagcta gaatagaaaa agctttggaa aaaatagaac aggaaacaga
     4801 tcaaccagtg attaaagatt tacgtgaaat ggataaatat attgattata tgacacgtaa
     4861 aaaagctatc ttagaaaatg tagataatga aatagtaaac ggagcattta gcaatacaaa
     4921 aagatatatc actaaaaaag acatttcaaa aataaaagaa tcggtcatag aagcaaaaaa
     4981 taatggatcg gtgatgttcc aagatgtaat ttcattcgat aatgattttt tagtaaggga
     5041 aggatattat aatccagaaa caaatgaatt aaatgaaaat gttctatatg aagcaacaaa
     5101 gtctatgatg ggaaaaatgc aagaaaagga agaactagtt gatccttttt ggtttgcaac
     5161 gattcacaga aatactgaac atatccatat tcatgtaaca gctatggagc gtaaaaatac
     5221 tcgtgaaata atggaatatg atggtgtttt acaagctagg ggtaagagaa aacagtctac
     5281 gttagacgat atgattttta aattcggtag caaaatatta gatagaacga acgagtttga
     5341 aaaaatttct aagttgagaa aagaagttcc tttagaactt aaacagtctg taaaagattc
     5401 tttgattcaa ttatatgtag aaaataattc aagatatgac aaagaattag taaagtacct
     5461 aaaggaatta aaaaaagaaa tacctagtac aacacgtggc tacaatgagc ttcctgaaga
     5521 aacaaaagaa aagattgatg aagtcacaaa ttatatgaca aaggataatc caaaaagaaa
     5581 agaatatgat cgtatgacta aagaaattga tgagctatat caaacaacct acggtaaacg
     5641 ctacaatcca caagaatatt atttaaatag aaaaaaagat tttaatgctc gaatgggtaa
     5701 tgctgtagta ggacaaatca aacttttaaa gcaatctgaa gaaaaaaatt tgaaaagtaa
     5761 tgaacttaaa aatctattag acggaaaaaa attaaacgtt aagtttgata gagaaaaacc
     5821 agaatttaaa acgaaatatg ctaaacaaag ttatgatgat ttccaaaaaa gattagaaga
     5881 acgacaatta gcacgtgaaa aaaatgaagt ccacttcaag aagatttctg aaaaatttaa
     5941 agataaaaag cttattagaa aaattgatag agtaatcaat gatgatttaa atatgtatcg
     6001 tgcgaaaaat gattatgaag tagcgcaacg aatgatagag caagagaaaa tgagacaagc
     6061 acgtgaattt gaaataggaa tgaattagta gaaacggaga atagaaatta tgaaaaactt
     6121 cgatatagtg tgcagtaata caaaaaatat atatttacgt gagctactta acagtgattc
     6181 ggaaactata gaagatgtaa aaaaaatcat agtgttgttt gaaaaagaaa acatggagct
     6241 agagaattgg gggctttttg aaataccgat tagtgggaat tactgttttt ataattggaa
     6301 gacagaagac gatgtagctt ttgcaaatta cttttttgat aaaaattact tttccccatt
     6361 atatatagat aagcatagta atgaacaagt tgcttcttca ataaaagaag caataaaatt
     6421 ggaaagagtg aggaaatgat atgatcgatt ttagaacggt tatggataaa ctaagtaatg
     6481 gagattttcg cgtattgatt gaactatact taaatgtaac gtctacagtc aaagagtttt
     6541 tatattctaa tcccaaaaca gctttagtat tagttgcagt actgatagtt tttgctgtga
     6601 tctcttttat taagaaaatg attccgctat taattatagg tgctgtgttg atattaggtt
     6661 tgtatttccc tgaagttttg gatcaaataa aaaatatatt ttaggctcta aaagcttcca
     6721 gtagaaaatt ttgggctttg atatttgtat taagcggttt gaaatagaaa tttgataaac
     6781 tacatgaaaa gacattatat aagagtatct gtgtattgga ataaaaatat gataatataa
     6841 aattatatat cagaccgata aaaaagaggt gcttataaat gaaaaagtta agagttattg
     6901 caacaatacc tatgattttt agttttgcgc tattattatc tgcatgtggc aaagaagaag
     6961 ctggtgctaa gtcaaatgaa gcacataata tctcattcat agatagtgca gaacaaaaag
     7021 actcacgttg ttggttggta gtcgaaacaa gtagtaaaaa taaaacgcct attgataaaa
     7081 atgaatatgt aaaagcggtg atccaaacta aagatggcaa agcaaaagta tttaaaacgg
     7141 atatggaaca gcttacattg gaaaaagttt ctaaaatgag tgatgaagaa ataatagaac
     7201 aagcattgaa cgctgataaa caaaagttta atgataagaa aaaaagcgat attgatagct
     7261 ataatgccac tttagattct gtaaaaacag aattagaaaa tgaggaaaaa caagagtttc
     7321 aaaatgacca aaaaattgct gattttcaag atagtatacc taaaatacag gcggatatag
     7381 aagagatcga tggtgttcaa tattcagagc cggagtggaa aatggtatca gcaacaatta
     7441 aaatggataa tgaaaacaaa tcaccttcaa tggaaaccgt taaattatct tataaatatt
     7501 tagatagtcc aatttggaat ttaaatagtt ttggtgaagt ttcgtctgaa ggagaattat
     7561 cttatgaatc tactagtatg gatttgaagt tatcaattcc agttgccaca acgaaagttt
     7621 tagataattt ttattcagga tattctaatg atgaattgga tcaacttttg cttactaaag
     7681 tagcttctaa agataacaaa gttcaattag atagtaaagg taaaaacgta agtatagttg
     7741 agtagatggt aaaataagaa gggataagat ggttactata actatcttat ctctttttta
     7801 tttgcattaa gtcagtgaaa ttagatagaa gagtttttat atatctggta taatttaatt
     7861 aggtaattta acttacctaa aaaagaaagg agatagaaga tggcctataa aaaaggaaac
     7921 tggaagccga aaacgaaaga gcaaaaacaa aaagagctag acgatttaat taaggcttcc
     7981 aacagcaaaa tcgagcaata tcagtctaac cctgaagata tgatcgagtt tgccaagttc
     8041 atgtctaaaa tacacaatta cagtccttta aacctatcgt tgatcgatga gggatttgaa
     8101 ggagcaattg ctgtagcaag ttatgatggt tggaaaaaac taggatttca agttaaagcg
     8161 aaagaaaaag ggataggcat ttatgcgcat gctccggtaa cggtttttat tgatgaaaat
     8221 ggtgaggaaa agacgttaaa agaagcgaca aacgagcaaa aagaaaaaat caaaaatgga
     8281 gaattaagaa gcaaaaagat caagcatttt aagaagggtt acgtatttga tatttcccaa
     8341 actaatgcaa ctgaagctga tttgccaaaa atatttccta acagggtatg gaattttaat
     8401 attgatggtg aattaagtgt tagtcaatta gaaaaaggtg taaatcattt ggccgaatct
     8461 atgaatatat ctataaaaga tatgaacgag tctggtagag gtgaaatggg aagcgctaga
     8521 ggtgaatata ttcaatatgt tgatggtagg gaagaaattg tattaaattc aagaaatagc
     8581 agaacacaaa atcttactac cagtattcac gaattggctc acaaaaagct tcacaataag
     8641 aatgaacacg gttcttccta ctcaacaccg attaaagagt ttcaagcgga aatgacttca
     8701 tttattgttt gtcacaatta cggtattgat acgtcagact ttactattcc ttatattgca
     8761 aattggacta aaaataatga aaaaattgat ccaaaaaact ttagacagat aatgcaagaa
     8821 gttagagaaa cgtctattga ctttataagt acgattgata agaaagtctt ggaagaacag
     8881 caaacagcat ttaatgcact aaaaaattcc tttgttccta atgagaataa agagcagata
     8941 caacaaaaat cagatttaga atttcataat ttctacgata gtacaatcaa cgatccggat
     9001 agaacaatcc acggtagtta tttagaagcg atggtggatt attatagagg aaagtatgga
     9061 gaaaattcta ttgagcttac tcatagcaat tatgatgtta cagctaaaat aggagaaaac
     9121 tatgtgacat attattcaga tacgaaacaa tttgatgtat gtgatgttta tggtgtagtt
     9181 tttgagaatc caagtgaaaa taaacatgat cgtttactat tagatcgatc acctgaaaat
     9241 tgtgattatg ttgaaaagtt tgttgtagaa agtttgaaag cttgcgagaa tcatgaacta
     9301 aaaaatgata cgttaaaatt aagcgaaccg ctaagatcag ttgttataaa tgaagcggtt
     9361 tataatctat ctgaaaatgc taacaattac tataatgaaa aatgctggca agaagaaatt
     9421 gaaaaccaaa caaatattaa tgatgtaaaa gaattaaact cttgtatgac tgcggaagat
     9481 ttttacgaat ctcataaaca agaaatcgaa aatacgatta cagattttga atctgtgtca
     9541 ggaacaaatt taagagatac aatagacaat gagtcatata aagaggtggc ttcagtggta
     9601 gcttataaac tggttgcaac taaagtaaaa tatgaaatgg atgaaggaac tttcaagata
     9661 ccagaaaatt ctaacttgga aaagccgact gtaaaaacag cagaacgttc ttttgaaatg
     9721 gagtgaaaaa aattatgagt aatgaacaaa attatggaac aggcggaact tatcgcctgt
     9781 ttcatggaag tatgaataaa tttgatgaat ttagtttaga tcataaaggt ttaaacggaa
     9841 cagctaatgg atttggtgtg tacttaacac ctaataaaga aatggcgaca atgtacgctg
     9901 aacaaggtag taacggaaat aatggtttcg tatatgaagt aaatgttgaa ttagagaagc
     9961 cgatttctat gaatgaacgc actattactg atgaagagct ttctaaagta atagataaat
    10021 tacaagaaag taatgatatt ttaaatgatt acaatgatgt tagttattac ggagaaaaag
    10081 ttgtaaaaag agaagtgatt gcttctctta gtgagaatga aaatgacgta gatttgatta
    10141 acgatattgg aaatacaatt ggagatcatg aagctactgc taaggcattt tatgatgttg
    10201 ggaaatatac acatattatt gcggaagaac agttaagaca aaaggatgta gttatcgttg
    10261 tgttacagcc tgataaagta aagattgaaa ctattcacga tttaaacgag gaacggaata
    10321 gcttagaaag tagtgaggac atgacagata aatttgttgg gcataaggtt tcggaagttc
    10381 ctattcgaga agttgtgata aaagattttg agcaagagct agaaacaact aattattatg
    10441 acaaacaagc ttggcaagag cgcattgaat acttagaaaa catggaagaa ccagtttcca
    10501 gattagcatt agatagtgat gttaatgaat tttatgatag tcataaagaa gaaattgaaa
    10561 cgaaattgac aaatttatcc gaaaagttag agatcgataa agaagaactg ctaggttttc
    10621 cagtagatac ttcttctacg gaatacaaaa aagctacgtc tatcatggct tacaacggtg
    10681 caatgagaga aatttatgat gaaattgaaa atgatacgtt tgaaatacca cctgtttctg
    10741 aagaaatgga gaataagatt ttgaaattga ctaattttga aaatacaaca aaatttgaaa
    10801 tggagtagat aaatgaatga taggacaaga aaatagaaat tttttagaaa cgcaaggtga
    10861 atatcgtcaa attaataata gtcattttgt aatttgtgaa gatgtacaaa tcgcttttaa
    10921 aatggctttg aagtacggaa aaagtattag aaaaaagata attagagaaa aaaagagcaa
    10981 gaagataggg ttttgggagt tatctcttag ggatgggcta aactttattt tctgtacaga
    11041 agattattat gaaaaataca agtgtacctt atcgtaattt tactagtgat ggtctagtaa
    11101 agggggtttt tgtttggatt tgaaaaaggc aaaaaaagta agtatcgttg atttttgcaa
    11161 acaaaatggg ataggtttaa agcaagattc aagggaatac tatagattag aagatcatga
    11221 ttcttgtgta atcagtgatc gcaaaaatct ttttaaatgg aactcaaagg atttaggggg
    11281 agatattttt gattttatca aagcctacta tgattgttca tttaaagaag caaaacagaa
    11341 acttgaagat agtgattatg aagttcattc gtttaataca aaagtgaaaa aatctccgta
    11401 ccaatatcag aaagaaaatg aagtgccggt atctgttgca aagtcttatt tagtaaatga
    11461 gagaagaatc gatatagctt tagtggaaga ttttttatct aaaggactaa ttaaacaaga
    11521 taaaaaagga aatgttttgt tcttatggaa aaaagatcaa gaaatcgttg ggtgtacgga
    11581 acaaggaact agaaaatttt ttcatgaagg caaacaaaag gatgttacat ggaaaaaaat
    11641 acaagaaaac tctaaagagt attcaggatt taacgtaact tttggtcaac cagataaaat
    11701 gtattttttt gaaagtgaaa tagatttgat gtcgtacatt acacaaaagc cagaaaaagc
    11761 cgaaaatgct acgtttgtta gcatgaatgg attaaaggta ggtaatgtat tgcatggggt
    11821 tacagagcat tataataagt ttaaatcatt gccttcagaa acagtccttt gtgttgataa
    11881 tgataaagcc ggacgtactt ttattgaaac tgaatttaaa gaaaaaggaa ttggaagaga
    11941 agggatagga atcagtgaag taaaagtcga tgtacctttg cgagagggat ttgattggaa
    12001 tgataattta acaagaggaa aagaagcttt taaacgcttc aagttagaag atgtatcaaa
    12061 aatcgtgaac ttcacacata aattatctct taatactcct gtatatgaaa tggaataaca
    12121 agaagaaagt agttgatgtg ttatgaacga tacaatagca atcttatcag attcattaat
    12181 tgatttagat aagacgtcta cagactatat gattattgaa gaacaactta aaaataaaaa
    12241 tccagagttc gatattcaag aaaaatcatt gcgtattgaa ttaccaaaat ttaaacaaaa
    12301 aaaattaata actattctct ttgagattat tgaacgatat gggcttactt attatcaaat
    12361 ttggacggat aagaaagatg gccaatatac aaaaggggta agcatagaag ccgttagaga
    12421 agacgatcag gtaagtatta aagcagttca acccttaatg gggtataacg aagaattatt
    12481 gaattgttta aacgtctagt gtaaactagg cgttttttta cattatttgg ctacgaatag
    12541 acagttaatt aaactaaaga aaactattga ctacaagtag tcattcctgt atgatatatc
    12601 catagactac aagtagtcaa agactcaaac ccttgattta ataaaaagga gagataatag
    12661 atggagcaga caagaatgtt ttggtcagat cgaatgcaag atcacttgga ttgtatttac
    12721 aaaaccagag agcaattaaa taagttagat actgtcagat attattttac ctacttcagt
    12781 gagaatgacg aacaaagatg ggatattttt catgatggta attacgaaga atttttttac
    12841 actgaaagtt aattatttgt tgaaatgatg aaggattcta tttgatgaaa gggaacggaa
    12901 aaccgttccc ctaaaaaata ttcaaatgac tacaagtagt catagaaaga gagcggaata
    12961 atggtaaata aaaaagaaac gtttaaagta gggatagaag tacagggcgt tgttattggg
    13021 agcggagagc ttatcgaggg agaagtaatt agtttgttag ctaatgtagc ggtagttaaa
    13081 gcaatcggaa aagcaccggt agtgatgaaa aaatgcagca tgatgtgtct tgatggttta
    13141 aacaatccaa taactcaacg ctttgagaac gtgatataat agaaaaaaga agattttcta
    13201 ctgggagaga ttattatgaa agactatgct tcacgtaaat ttatccactt agagcctatg
    13261 aaagaattgg ttattaatgg gaatgaatat aatgattaca aatctttatt tgtaaaggaa
    13321 cagcctaaag gtggtattcg gatgaaaaat ttaccggact tccgacttat tgaagtaaac
    13381 aatttaggaa ttatagaaga ctatttagct aagttagatg aagcggtaaa acaatcaatt
    13441 gaaaaaaatg gtgaagacca tgtacttacg gttgtcttaa aagacttgaa agatagacta
    13501 gaagctgaag cagagaactt taataaagac taataagaag ggaatgagag atatgaccga
    13561 ggctgtaaaa gaattaaaga aaatgtatcc tgatgttttg aatatgactg tagatgattt
    13621 tcacgaagcc ttaaaaaatg cagaaagtga ggaagaaagg actttctacc ttacactttc
    13681 tagttttgtg acacgagtag atcaaaaaaa agtgattaat cagaaagatt ttaaaatatg
    13741 agtttaggaa atgagaaacc atattatgta gttattgggg gagtaaacgg cgccggtaag
    13801 tctactattt atcaaattca aaatttaaaa agaaaagaac ttgatactga tagaattaat
    13861 gcagatgaaa ttttgagaag tttcggcgga aattggaaaa aacaagcaga tataaacaaa
    13921 agtatgcgtg aagttataag agagatcaaa gatcatttta aagaaggaaa atcttttcac
    13981 cacgaaacag cgtttgttgg taatgaatat ccccataaaa agagacttct aaatgcaaaa
    14041 aaattaggct atgaatcatt tttaatctat gtagggttag attcaccaga aaaggcgatt
    14101 gaaagggtta aaagtcgtgt tgctaaaggt ggtcatgggg taggcgaaga tttaataagg
    14161 gaacgatacg tatcttctct aagaaactta gcaggattag aaaaatattt tgataatgta
    14221 agtatctatg ataattcaga tgagttttct tatatttatg ttaaagaaaa tcatacagtt
    14281 gtagccaata atacgcaaga atataattgg gttcccaaag ctattcaaga aaaaagagct
    14341 gaaattccta taagtaaaca aattatcaag gatatatccc aagaagggtt gagtccaaaa
    14401 gaaacccttg aaagaatata ttcagaaagt gatgatttta ttagtgatcg tttttttaaa
    14461 gcagagcaag ttgatgattt ttatatgaaa cataaaaatg aaatcaagga acgggttcta
    14521 gcaattagtt tttctacagg agtatctgaa gatgaattag taggccaaga taaaaaaaga
    14581 aattcttgta agttggcata tattaatagt gtaggcgagc ttttagaaaa aactgatagt
    14641 gagctatcga gcatggaagc aagcagagat agaagtataa aaaaaactga ttttgaaatt
    14701 gaataaaaga agatggagct gaatcagtta atagtaaata aatttaataa ggagtgcgaa
    14761 agtacttctt attttttaaa taaaatgact acatgtagac gaaagggata atatattatt
    14821 tttagttctt gtttttaaaa ttactatttg ctataatgaa cttaaataaa tttttctcag
    14881 aattttattt gctataatag actcaaataa agttttctta gaactttatt cattatcgag
    14941 tgttcccctt tgtctgcaaa actatcggga gcactctttt tcattttttc aataagttct
    15001 gtaaccatat agaacagcac gcttctgaaa tgtgtagata aacagctctt aaatatttat
    15061 gagtcagtat atcatttaag tcttagttac atgctaagac tatttttttg ttttgtgtgt
    15121 tacgtaaggg tataccctat tgtatcatat ggtaaagtac agtataataa ggtttggagg
    15181 tgtaacatta gggtgtaaca caatgtgaaa tttggaggtc attttttgaa caagaaacag
    15241 gatcaagaat attatataga aaaattggag aaagagaact tagaactcaa agagcgaata
    15301 agatactatg aatccaaatt ccataagaga agtgattgta tgaaaccaaa tcttattgaa
    15361 acaggtaaaa gaattaaatc aattcgttct aatttgggaa tgacaatgga gcaattcgct
    15421 attttaactg attcttctaa tacgagtgct gttaataatt gggaacgtgg ttacaactta
    15481 cccaataaaa ccaaactaaa aaaaatagct atactaggaa atacaactac tgactggatt
    15541 aaatggggaa ctttagaaga atatataact tcatacctta tcggtatagg atatgagcta
    15601 tatattaaag actttccaga gatacctcac aaagtcttta aagacattca agaaaaatat
    15661 agtgatacgt tttcgttaga taaagactat gaattattgg attctataat aaaaaatata
    15721 tttacaaaat attactcaaa agaatttgaa gtaaaccaaa ttccataaga gaggtgattg
    15781 tatgaaacca aatcctgttg aaacaggtaa aagaattaaa caattacgcc tttcatgtgg
    15841 ttttactatg gaagaacttg gaaggaaaat agataattca ccacgtgcga ccatatccaa
    15901 ctgggaacgt gggaccaatt tgccaaatcc acaaaagctg aaattattgt caactatcac
    15961 taattcaaca attgactgga ttaaatgggg aactttggaa gaatacatag cttcatacct
    16021 taaagattca gggtatgaac tatatattaa agacttccca gagatacctc acaaagtctt
    16081 taatgacatt caagaaagat atagtaatac gttttcgcta aataaagact atgagttatt
    16141 gaatcctata ataaaaaata tatttacaaa atattactca aaagattttg aagattatcg
    16201 agacatagaa gttaaaccag accctaaaaa ggtagggaga aaaattcgtt ctattagaaa
    16261 aaaattaggc cttactatgc aggaatttgg gtgtgaggtt tctaattctc ctagaagcac
    16321 tgtgtcaaca tgggaacatg gtggtaactt accgaacaaa gccaaactaa aaaaaattgc
    16381 agacattgcg aattgtagtg tagaagattt gctttttgat gaaggcttta tttctgaaaa
    16441 atcccaagtt aaattgataa atgaactcaa agaagaaaat tcaaagctaa aagaaaaaat
    16501 aaaatactat gaatgtttat ttgatggtat agagagtctg ctgaactcac gaaaaaattc
    16561 ctagttaatt aaactagagt aaaattattt gtaggtgtta aatatgttag tgggttatgc
    16621 acgggtcagt tcagatgatc aaaacttaga aagacagctt gaagaattta gaaaaattgg
    16681 tgttgaaaaa gtatttgctg agaaaaaatc tggccgtaac acagtagaac ggccagagtt
    16741 caataaaatg ctagcatttg ttagagaagg agacacgctt gtttttgagt ctcttgaacg
    16801 gttaggaaga aattcagatg aaatactcga tacagtatct tttatagata aaaatggtat
    16861 gggcttaatg gttttgaatt taccaatttt agatacaaaa tttggtgatc caaacttaga
    16921 aaaacttgtt agaagcttag taatcaacat tttttcttgg acggctcaaa atgagaggga
    16981 ggaaattaag agaaaacaaa aacaaggaat agcaattgcg aagaagaaag gagtctataa
    17041 aggaaaaccc tacgaatatt cttctactgc aaagaatcca caaaagaggt ttatctataa
    17101 tgaaatagta agaatgcttg gagaaggtgt tccgattaag cgaattgcag aaaaaacagg
    17161 aacaaataga aatttagtct atagaataaa gaaaagaata agtgaatctg attgatttta
    17221 agttctttga gaaatcaatg ttgcgagagg acgattctaa gcctttttcg agcttttaag
    17281 atgtacaatt atgcaaagaa accttttgat gaaagagtat gatagttttc taacgaagga
    17341 aacagcctac aaatggggta tcaaggagga tttttatggg atttgattta cttgtatata
    17401 gtggaaacaa agaaaagaag tagatgatct aataggaaag gaagtagtca aaaatggcgt
    17461 atgaaacaag tgagggaaaa gaaatcaaaa cagtagaaat cgaggtgggt aaagtacaac
    17521 agctttttga caatttcgca cagaaattac cagaaagtct gctaaaagaa ttggacgatt
    17581 tagaatatga gataatagga atggcaaggg taatttcggt acgtgattct aacgagcaat
    17641 agtaaagagg aaatttcggc actattacat gtagaagata aaacaaatga aaacccctct
    17701 tagtttccgc taaaagggta ggtaattagg aggaagctag aattggagat tctttatgga
    17761 atactaagag ctattgctgt tattttgggt gaaattatag agagcttaac aggcttaata
    17821 aatagtattt ttggaactat gctaggaaag cttggtttgg taattctgtt aatagtagct
    17881 atgtgtttgg ataattattt tagaaaaaaa gagagaagaa taaatgagta agtgtgctaa
    17941 cggaagagat aatggaatag gaggaagaat tcaaatgcaa ttaaaaaata gaattgaagt
    18001 agaaacccct tattatttta aaggatcaag ttgcttatca ttgtttattg aacaggtcga
    18061 aggagaacat gatcaataca atctcagcag tgaatatgag cgcttgttta ctggaattcc
    18121 tttcggggaa gtaattaatg aatacaaaaa aataaaaagt gacttttatg cttgaagttc
    18181 atgattgaca gagattgcgg aataggaggg ggcacatgcc gccagaagat tatcaaccgc
    18241 cattttgctt tgagtgtgac ggtgagatat taaaatcagg aaatttaatg gtatgcgaag
    18301 aatgtggaaa agtctatacg catgaagaat accaaaaact gactgatgaa gaagcagaaa
    18361 aatatataga cttcatgaag aaaaaatcgc cagaattatt cggttaatgg aagaaataat
    18421 ggaatggggg atggaaatgc cacaaattag agttgaatgt agatattgcg ataatccttg
    18481 taaacctaga aacgttgatg gtgacttagt atgcagtaat tgcggtgccg agtgggcttc
    18541 agcaaaatgt gaaatcaaag tttctgatcg ggaattagag cgtgaatgta aagaacaagc
    18601 cgaattcgat cagtggatgg cacagtatgg tgaagattaa agagtgagaa tatgggggag
    18661 cttatttatt tagtattata cttaatagga aaatcgactg attttgtata caacaataac
    18721 gtaatattta tattgttcgt aattgtctgt attatcatgt ttgatgtcta tgatttatta
    18781 aatcatagaa acgaaaatag caatgttggc agggttataa tcacaaactt aatactaatt
    18841 actatcggta ttacaacatt aacgaataaa gcattaatat ccagtatgat tttaatagtt
    18901 tttgacttca ttatcattta tagtttgcta tttttagtca agaaagtaac tgaatatttt
    18961 aatcgatagg cactaaaaaa ctttaatata ggttgaataa tagcagtaaa tcatacaaaa
    19021 tagaaatgta gtcttgttaa gcgtcttgac tatctaattt tgaatgagga gggttgtata
    19081 tatatatata aggtagttaa attcgtagat caaaaggcta cgaaggagaa taaagaatga
    19141 cagctattta taaagcacta aaaaattcta acgtatcaga gtacgttaag tcgagagtat
    19201 taaataataa tttagattat attttggaaa atggtgttgc tgatttaatc agcaaattaa
    19261 gtgatcctat tgatatagat catttggtgg tgtggcaaga tggcacgtta gaggtatcaa
    19321 tatatggaaa attctatagt agaaataaag taactacatt caaaaatagt ataagtggtc
    19381 gggaagattt aaaaggaatc aataaaaata gtaaattcta tagaacaaat aaagagttgc
    19441 tagaaaaaat ttcaaagtgt gtttctcaat tggtaaatcc attttcaaaa aaacgtagta
    19501 aagaactttt tggaatattt tatgatggat tcaatgcaaa aagcaatgta gaaaaatttt
    19561 tttattttaa tgattcagct tacattgcgg ttaaagacag taaaagaatt cctgaagcaa
    19621 ctttagaata tttagagcca attaaattat ccgtttttta ttcactttat gagaaagaaa
    19681 ttgctaatga agcgatttaa gggcagtaga gtgagtaaaa aagtaggcaa agagttatta
    19741 tcgctaggat taaaaaaaga aatcgttaaa tttagaaata tgtatagaga accggaattt
    19801 agactcattt ttcaattaga tatagtgaga ggtctcaaaa ttagttttag ttgcttaggt
    19861 tatctggtct ggctattcat tgctagagta ggatggagta atgcttatag cttttataaa
    19921 ctgttttccg gaactgatcg attaaaagaa tcgctagagg tgaaagaagc tttaaataat
    19981 ttgggaagta tagcttttag cctattcatt tttgctttat ttatcttaca agtaggaaaa
    20041 atcatgatgt caacaaatga taattatatt gactatttga agccttttca aaaagggata
    20101 aaaactattt gtagacaagg cactgtttta tttctacttg gtctatatga gcatgaagtt
    20161 cattttcaaa attattataa tacaacagct aaacttggtt cgtttttact cctatattta
    20221 gttagtaata tagctgaaaa aatactgttt aaatatattg taaagcaagt cttttcagat
    20281 attcaaaagg aaagctactg cttaaaaaca ccgtatggga atttaaaaaa tgttgatcta
    20341 tttttgttta aaatagatag taagtgggat aaatatatct atagaagtaa agaagtattt
    20401 ttaactggta gttataacga aatccttcaa gtgagtttgt atgacacagg agcaatattt
    20461 aataatgaac ctatggtttt gaaagatttc tttggagatt atgaattgat ttggaatcca
    20521 aaacagttta gtgaacaggg ggcagaaaat gaatttgctg aagttcccta acatagtgta
    20581 cagaaaaaca ctatctgaaa gaagatcgcc gtctaaagaa attataaaaa caatctggcc
    20641 ttacaggaga ctaacaaaag attgcgaaaa aaaggaaaga caatgaaaat gtctttcctt
    20701 tttatttgac tacaagtagc ctgtaaatgg agtttccaaa agactacttg tagtcttttt
    20761 tgcaaaaata aagagccaac gaaagctacc aacgatcgtt gactcgcaag actgttcgta
    20821 gtaagaaaaa ctttggcaat ccacgaagtc tttatttcat aatttatata tatgattata
    20881 cagccattgt ctcaattttg caagtaaaaa agttaagaaa aattgtttaa gcgtttactt
    20941 aaaataatct ttagatacaa atttaagcga ttggaagtat gactacaagt agccaaaatc
    21001 tttcgttcag cgttgactac aagtagtcaa aatggtataa tatgtatatc aagagaggag
    21061 aaacagaaat gtataacgta ggggatcaaa tagatgaggt tgtaaaaata ggcaatgtta
    21121 aacttgataa aagtaagtac tatatttcag aaactgaaat aacattcaat actgaatcag
    21181 gatgtactct aatttggaga ttgccttttc caaaagacga agcaacactg aaaaaaataa
    21241 atactttaaa gattagatat tgcatttatg aagttattga taaaaatact tatgttatca
    21301 ctgaagtaaa agtgatagga taaaaatctt tttaaaaaaa actcttgtta aaagtaattc
    21361 tattagctta taatgttggt attagttctg aagccaacgg aacactaaaa aattctagta
    21421 gaaaagttag ttacagactt tctactatgt atgaatgtaa cgaaaaaaat agcgaaaaag
    21481 ataattaaat aagactacca atcttattta atggaacttc tgtagtaaga aaaacgattt
    21541 ggcaatccac gaagtccacc aagatgagct accaactcat tttaattttt gcacgtttaa
    21601 agtttagcat ttaagtgctt actctaaaga catagtgtaa ttttaggact ccttattacg
    21661 gaaactaaag ttatcctatg tcttttttgc tattcaaaat tgatagcaag gagaaagtgc
    21721 aatgttgaat agttgcgagt atgcggacat tttagctaaa attcttaaaa atggaattaa
    21781 acagtttgat agcaaaaatc tagttgcatt taaaaagaat agtaataaga tcggcgctat
    21841 tgccggattt acttctaaaa acaacatgat aaagactaaa ggtcttatat atcgttctat
    21901 tgatcgtgtt ttagcagatt cagatgtttt aacacactgg acccctaatg tgtattcatg
    21961 gcttggtggc ggtgaaaata aaacgatttt tggtcacaaa gaagctaact taattcaaat
    22021 taatactttt gtagcagata ttgactatcc gataggagaa aagaaaacgg acttgaattt
    22081 actagttctt tatctattgg aagagggatt attgcctaca ctgattgtag atacaccaaa
    22141 aggttatcat tgctatttta tgattcaaaa ctataaccaa gaaaatgatt catttgataa
    22201 ggcaagctat atctctaata cgaatgattt taaatctctg agagttgcta aaagaatatc
    22261 agaaaatatt agaaaatcta tcaaaaatcg tttgccacaa gttgatatgg gctgtaatca
    22321 ttttggaatt ttcagatttc caactaaaaa aaatattgta cattatgaac ccaatttcgt
    22381 agatacgttt gaagggtatt tgaaatggtc aaaagacttt gaaaaaaaag aatatgcttt
    22441 aaagaggtct aacttgtcat tattagctcc taattatacc ggtaagaaag ggtctacacg
    22501 acaaattgat actaaatggt acgacttttt aattcatacg gatattgctg aaggggatag
    22561 aaataaaact atttttacct tagccctggc atgtaaatca agcggcttat ctattggcga
    22621 gtgtatggat caattggatc aagttcaatt taataatgat ttgtcctatt ctgaagttga
    22681 gagaacaata agaagcgctt atcgtggtga ttacaaagga gctaaaagct catatgtcaa
    22741 tgagattatc gaaaattatg ctacgcctac cgaatttgaa aaatactcaa taaatgtaaa
    22801 tggacaagct gttaaacgta aaggaaatcg tgtcgatcct tctaactggg tgaaatttgc
    22861 taaacctaga gaagaacgaa aatatagtca ctttgaagaa agtaaagctg atttactagc
    22921 ttacttagaa agaaaacaaa gatcattgaa aaagaatgaa ttgcttttaa ctatctctat
    22981 ggcgactata tctaaagaaa cactaatccc tgtatctacg ttaaaggtga ttctaaaaga
    23041 gcttagaaca gcgaatacag tgatattaca aacgtctcgt ggtcgatatg gatcgactaa
    23101 aatagcaaca agtaaatttg tggaagctaa aatattaaat gcagttattc agaataaaaa
    23161 agataaacaa gtatttgtct tagaattatt aggaacagca aataaacacg tagaaacggt
    23221 tttaaggcgt tatgaggaca aaacagtacc attccatacg aacatactga aagagcgaga
    23281 aaagagcaaa catgcgccaa ttagaggagc taatacaggt taaacctata tttatttttt
    23341 agatttttaa atagctaaat tgccaattaa catatcttgt gcttttgctg cttaccacgt
    23401 cctgttttaa cgtatactat tcttagtatt ctcttttatg tagggggaga aaatattctt
    23461 atttgtgtct atcctttgag caacgtgtac ggaatatact ctataactgc gtatgcagtt
    23521 atagttggag gaggaagatt tggtgtgtct gtaaagaaat atgtttactc tcacccgata
    23581 aatgtattta ttatcggtaa gttaggttta tctgttgaag tgttttgtga gttatatgga
    23641 tttaaacaag gtactttatc gtcttggatt actagagatc gtgagataga tgattttcct
    23701 gtttcttttt tgtatgcttt gtctttagct tcaggagaga atatgaatga ggtttatgat
    23761 tgtttaagtg agtatgaaaa agagtataaa gagtacttaa aagtttcagc accaaagcga
    23821 aagaaaaaat atattgaaga atagggaagc aagaagaaaa tagtttgttt tttgactaca
    23881 agtagactga tagtaactct gcttttgata atttgatatt gtctgttttt ttgttagaat
    23941 taatatatat caggtatata aaaaaggagg ctattaatga atattgatgg gatttataat
    24001 gaatcagatt ttaaaaaagg tatggtagat attgatagaa aagagtattt atttcctgat
    24061 gaagtaggtt tttttaaaat tgaaggtgaa actgtctgta ggtattgggg gaagaaggga
    24121 aacataattt tattggttca tacggttgaa ggaagaaaat taaaattttc cttttggagt
    24181 aatacaaaat attgttgtga gcctgtacct aaaaggaaaa tagatgctgc taaagaaagt
    24241 gtgcgatttt tacctgatcg ttcaaaaata actttaatat gtgaacgatc tagtaatgct
    24301 aaatttacga gaattagaga tatattgttt gagactacaa gtagtcaata gagtaattat
    24361 ttttggttaa aaatcgattg aagaaagtgg aggaagtata tggccgtagt tatattagca
    24421 gaaaaaccag atcaaggagc aaaatttgct tcagcattgg ccggaaaaaa attatcgaaa
    24481 aagagtggga agtatgaatt taaaagtgaa atatttggag atactattgt tacttgggga
    24541 ataggtcatt tggtaggatt atcattacct gaaaagtatg agtggctacc gaataaagat
    24601 aaatggcatt tagataattt gccttttcta cctaaattca atgaattaaa gtatgaagtc
    24661 tctaaaggaa aaaaccaaca attttcaata gtgaagtcat gtttagaaaa tgcagatgag
    24721 ataataattg ctactgatcc ggatagagaa ggagaaaata ttgcttataa tattttcaaa
    24781 ttatgcaagc agtcgatttt taataaaccg ataaaaagat tatggattaa ttctttggtt
    24841 caaaaagaaa ttatcaaagg atttaaaaat ctgcgaaatt ctgaagaaac gatctcttac
    24901 ttcaaagaag ctaatgctag acaaattgct gactatctag tagggatgaa ttatacagag
    24961 ttgtattcat taaagttgca agaaaaaggt atatcgggtg tggtatcact cggtcgagtt
    25021 cagacaccgg taaacacact tgtggttgaa aacgatttgg caattagaaa ttttagaccg
    25081 gaaaaatata aaacgattga atgtcataca accgataagc aaccaaaagc aatatttaag
    25141 aatagtcttg aatactttaa attagaaaaa tttgaagaag acactaaaaa attcggcctt
    25201 gattccgcca gtactggaat cataaaaaaa attgaggtta gtaaaaagca aacagaacca
    25261 caaaaattgt ttagcttggg tggtattcaa gaatacgcta ataaaaaatg gaaatattct
    25321 gctaaaaaaa cagataaaat tatccaaagt ctatatgatc gtggatattt atcttatcca
    25381 agaaccgata gtgaattgat aacaacaaat gaatttgagt atttaaaagc gaatttagac
    25441 gtgtataaga gtgttttagg gatcagtatt gatacacctt gtttagagcc taaaaagcga
    25501 tttgtagata atgacaaagt attagagcat tatgcactaa taccaacaat ggatattcct
    25561 gatatagcaa atctgacatt agatgaaaaa aatatttatg agatcgttac taggcgtgcg
    25621 tgtctaatgt ttgcagaacc ttacaagtac gaaaatacaa aagtcacatt agatgtaaac
    25681 ggcttagaat ttaccacaag tggtaatgta cctatatctt taggttggaa gcaagtagat
    25741 actaatttag atgaatctga tgataaagag gaaacattac ctaaatttca tgaacaagag
    25801 acaataaata tagatattag atttattgat aaattaacta aggaacctaa aaggttgact
    25861 gaaggtaaat tattaggtaa aaccggtatt atggcgaagt tagggctagg aacgcccgct
    25921 acacgttcta atattgtaga cactcttcta aatagggaat atattaagat agaaaacacg
    25981 aatgtttatc ctactccaaa gggatattta ttatgggatt tgacaaagaa taaagaccta
    26041 ttgattggga aaccagaaaa tacagcgata tgggaagaat cacttgggaa aattgcgcaa
    26101 ggcaactata ctcaggaagc ctttttaaga aatatttata actatatcaa tatgactgtt
    26161 tcagatttga agaaaataga atttacttct gattatttag aaaaaacggt aaatcaaaat
    26221 aaatttgata taggtaatta tcaagtagaa gaaaagaata aagttttttc cattttaaat
    26281 aatactagtg gtgaaaagtt cattatattt aaaagaatat caggaaaagc aatcaataaa
    26341 aaaatacttc aagaactatt acaaaataat cgtacagata aagtcctttc cggctttaaa
    26401 tcgaaagaag gaaaagcttt taatgcaaga ttacttttcg atccaaatgt aatgaaagtg
    26461 acgatggaat ttgaaaagaa cagtccaaat ggcgataaaa aaagcataca cgatatagta
    26521 aatggttatg aagtggttga aaaaacaaaa gtatttgaaa taaaggaact agctactggt
    26581 gatattttta ttttctataa aaacaatagt gggaaaacta tgagtttaaa aatggtgaaa
    26641 gagttattag aaaacgggaa aactaaagaa aaaataacag gatttatcag taaagacaat
    26701 aaaaaatata gtgctttcct tatttttgat agtaaaaata aaattataaa aaaagaattc
    26761 taatgcttga tggcagaaaa acaattgttt ttctgcctaa ttttattcaa aaatattgaa
    26821 aat
//
LOCUS       DACDSY010000019        25940 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDSY010000019 DACDSY010000000
VERSION     DACDSY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.019"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..192)
                     /locus_tag="IWK44_000727"
     CDS             complement(<1..192)
                     /locus_tag="IWK44_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP9549279.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSI"
     gene            342..824
                     /locus_tag="IWK44_000728"
     CDS             342..824
                     /locus_tag="IWK44_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP9549280.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            861..1871
                     /locus_tag="IWK44_000729"
     CDS             861..1871
                     /locus_tag="IWK44_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP9549281.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1986..2699)
                     /locus_tag="IWK44_000730"
     CDS             complement(1986..2699)
                     /locus_tag="IWK44_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549282.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(2889..3479)
                     /locus_tag="IWK44_000731"
     CDS             complement(2889..3479)
                     /locus_tag="IWK44_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP9549283.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            3963..4190
                     /locus_tag="IWK44_000732"
     CDS             3963..4190
                     /locus_tag="IWK44_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP9549284.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4206..5372
                     /locus_tag="IWK44_000733"
     CDS             4206..5372
                     /locus_tag="IWK44_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP9549285.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5606..6649
                     /gene="hrcA"
                     /locus_tag="IWK44_000734"
     CDS             5606..6649
                     /gene="hrcA"
                     /locus_tag="IWK44_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP9549286.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            6743..7306
                     /gene="grpE"
                     /locus_tag="IWK44_000735"
     CDS             6743..7306
                     /gene="grpE"
                     /locus_tag="IWK44_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP9549287.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7357..9186
                     /gene="dnaK"
                     /locus_tag="IWK44_000736"
     CDS             7357..9186
                     /gene="dnaK"
                     /locus_tag="IWK44_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP9549288.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9337..10503
                     /gene="dnaJ"
                     /locus_tag="IWK44_000737"
     CDS             9337..10503
                     /gene="dnaJ"
                     /locus_tag="IWK44_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP9549289.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(10585..11250)
                     /locus_tag="IWK44_000738"
     CDS             complement(10585..11250)
                     /locus_tag="IWK44_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP9549290.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            11226..11399
                     /locus_tag="IWK44_000739"
     CDS             11226..11399
                     /locus_tag="IWK44_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9549291.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11444..12181)
                     /locus_tag="IWK44_000740"
     CDS             complement(11444..12181)
                     /locus_tag="IWK44_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9549292.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12183..13685)
                     /locus_tag="IWK44_000741"
     CDS             complement(12183..13685)
                     /locus_tag="IWK44_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9549293.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13944..15938
                     /gene="uvrB"
                     /locus_tag="IWK44_000742"
     CDS             13944..15938
                     /gene="uvrB"
                     /locus_tag="IWK44_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP9549294.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            15948..18764
                     /gene="uvrA"
                     /locus_tag="IWK44_000743"
     CDS             15948..18764
                     /gene="uvrA"
                     /locus_tag="IWK44_000743"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP9549295.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19027..20292
                     /locus_tag="IWK44_000744"
     CDS             19027..20292
                     /locus_tag="IWK44_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP9549296.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20296..21027
                     /locus_tag="IWK44_000745"
     CDS             20296..21027
                     /locus_tag="IWK44_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP9549297.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            21162..22046
                     /gene="rapZ"
                     /locus_tag="IWK44_000746"
     CDS             21162..22046
                     /gene="rapZ"
                     /locus_tag="IWK44_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP9549298.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22043..23041
                     /locus_tag="IWK44_000747"
     CDS             22043..23041
                     /locus_tag="IWK44_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP9549299.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23075..24010
                     /gene="whiA"
                     /locus_tag="IWK44_000748"
     CDS             23075..24010
                     /gene="whiA"
                     /locus_tag="IWK44_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP9549300.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24553..25203
                     /locus_tag="IWK44_000749"
     CDS             24553..25203
                     /locus_tag="IWK44_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549301.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            25390..>25940
                     /locus_tag="IWK44_000750"
     CDS             25390..>25940
                     /locus_tag="IWK44_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP9549302.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGV"
BASE COUNT         8568 a         4381 c         5532 g         7459 t
ORIGIN      
        1 gatagaaaaa tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa
       61 cataggttgg ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc
      121 aaaaaggtct ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg
      181 aaatttttcc attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca
      241 atagttatgt ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg
      301 ataagatgtc tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga
      361 aattcttaca cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg
      421 aagatgcttt ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat
      481 tcttaaaaga taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg
      541 atttcaaagc attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca
      601 tggaagaaca aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga
      661 ttccgattat tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta
      721 gaccagatca aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg
      781 agatgccgat atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc
      841 atacttggag gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg
      901 gggttactgc accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt
      961 taaagagaat ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag
     1021 ataaggaaaa atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag
     1081 atgaaaatat ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg
     1141 attttgtcga ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta
     1201 cgttgctact tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta
     1261 ttgatttaag aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa
     1321 caagagaagg agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc
     1381 atgagcattc atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca
     1441 gcggagatgc agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc
     1501 atttaatcgg tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa
     1561 agccgatctt gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac
     1621 ttgttcagat gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa
     1681 gtgcggcaga ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg
     1741 aattaaaact accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat
     1801 tgattaatgg tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa
     1861 cgtttattta aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata
     1921 gaaaagaaag tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat aacatattag
     1981 aaagtctaat tatctataac tgtcacatcg attaactgtt tcacgtcatt gggatttcct
     2041 gtaggaatag ctactacaga tgcattatca atgatttctt gagttgtttc tctaatgaac
     2101 gctggataag atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg
     2161 attgtcccgt catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg
     2221 agcataacca catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt
     2281 cctgcaggat aatgaataac atgtaattcg aaatcacccg atgtatctct aaaagtatta
     2341 cctctaatag ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac
     2401 ggatctattg actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga agaaatattt
     2461 gtctcaactg tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt
     2521 gaagatgtat tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg
     2581 gtttgtctga ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga
     2641 gacgtacatc ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata
     2701 taaaatacct cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt
     2761 aaaaccttgt ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg
     2821 tgacaaataa tcatcagtaa aattacttgt cacactattg ataaggagta tttaattgaa
     2881 gagaatgatt aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg
     2941 tatagacgaa tcgttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc
     3001 aagtctgtga attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca
     3061 gcttgttgtt ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat
     3121 tgcgcatttc cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact
     3181 gccccacaaa tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat
     3241 aaatgatgat tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt
     3301 tcaggtattt gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc
     3361 tctgattgga tagaagcagt tttgttttgc catttctcat tttggtttag tgtgctagta
     3421 atataagcac cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt
     3481 ttcaagctct cctttactat ctataacaag gacaacagtt ttaacctaca gacattcatt
     3541 aaaattgggt agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagata
     3601 gtcaacttaa ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc
     3661 attataacat tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa
     3721 tattaaaaga aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg
     3781 cactcagaca tgaaaacaac cctagagata tataactagg taaccagttt aatgaaagaa
     3841 aaaatcatcc aagaagtcga ttcttggatt ttttagtgga tttgttcgcc aaatacgttt
     3901 agtatgcgta tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat
     3961 ctgtgaaaat agcttttcat atttttgcag attttgactt aatgccgaag gtcagctttg
     4021 cgattaccgt ttatcgagag gctgtaaaag caccgtgtag ctccgatgac aataagcaag
     4081 catttttaga gctaggtttc cttgattcta aatatgcggc ttatttcaat gagttggtgc
     4141 ttgaacgcga ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa
     4201 tttcaatgat agaaacaata aatcccgcag aaaacaaggt ttctgcgtac attcatatcc
     4261 cattttgcga gcatatctgc tattactgtg attttaataa agttttttta gaagggcagc
     4321 cagtagatga gtatgtggaa atgctgctga atgaaatgaa aataatgcta gaaagtcatc
     4381 cgacagcgaa tttagaaaca ctttacgtag ggggcgggac accaacttct ttatctgcta
     4441 aacaacttga tcgactgtta tcaggtgcta gagagatcct gccttttcaa aatggtaatg
     4501 agtttactgt agaagcgaat ccaggtgatt tgacccgtga aaaacttcaa gtaatgaaaa
     4561 actatggcgt caatcgttta tccatgggag tgcagacatt tgataatcgc ttattgaaaa
     4621 aaattggacg taaacacacc gcagaagatg tgtatcaaac tatgcaattt ttagaagcag
     4681 aaaattttac caatgtaagc atcgatttga tttatgcatt accgggacaa acactagaag
     4741 gatatctgga tactttggaa aaagctttgg ctttggatct gcctcactat tctttgtact
     4801 cattgatcct cgaaaacaaa acaatgttca tgaactgggt acgacaagga agactcgaac
     4861 tgcctgatca ggaaacggaa accagaatgt ttgaagagac gattgaagca atggaaaagc
     4921 atggacgtca ccaatatgaa ataagtaact ttgggctctc cggacatgaa tctcaacata
     4981 acctcatgta ctggaacaat gaccactatt ttgggtttgg agctggtgct agcggttatc
     5041 ttggagatat tcgttaccgc aacaaaggac caatccaaca ctatttacgc ccattacgag
     5101 caaatgaacg cccggtattg gaagaagaaa aattgacacg aaaaaatcaa atagaagaag
     5161 aaatgttctt aggactaaga aaaaaaacag ggattactaa aaaacatttc tatgatcgct
     5221 atcaaaaaac aattgaatcg gtgtacggtg atgtgctaag ggaattagag caagaagggt
     5281 tacttgttaa tgaagaagac cgtgtttatt tgacaccaag agggaccttt ttggggaaca
     5341 atgtgtttga gcgctttttg tttgaaaaat agtttaaaga atatttgttt tttatctgaa
     5401 tttaaataaa aaaagttcaa aaatacaata aaggactatt gaaacgttga tataactgga
     5461 tttacatcaa ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa atggagtctt
     5521 tctcttgaca ggagaagggc ttcttgctat atttataagt ggattagcac ttagaatata
     5581 tgagtgctaa tgagaggtga ggaagatgtt aacaaagcga caaaacgaca ttttacgtct
     5641 gatcatccaa cactatacga gtagtggcgt gcccgttggt tccaaaacat tgatgtctga
     5701 gggagtcaag gccagctcag caacgattcg caatgatatg aaagcattag aagatgaagg
     5761 attgctgttg aagactcact cttcttctgg gcggattcca tctgctttag gctatcgtta
     5821 ttacgtggat catttgttaa aacctgctcg cgtagcagaa gatgatttgc tgctgattcg
     5881 tcagtctcta ggcagagaat ttcatgaaat caatgagatt attaagcaat ctgctgagat
     5941 attatctgag ctgacaagct atacgacttt ttcattggga cctgaaataa aagaccgccg
     6001 actgactggc tttcgtatcg tacccttgaa taatcgacag attattgcta tcgtcgtaac
     6061 agatatgggg aatgtagaaa gccaagtgtt cactcttccc gaatatatgg gaagtcagga
     6121 actagaaaaa atggtacaga ttgtaaacga ccgtctagtc ggagatcctc tgatgacggt
     6181 gtaccataaa cttcgtacgg agattccgat gattcttcat aaatatttcc aaacgacaga
     6241 agggatgtct cagttatttg acaccgttct tggtcatgtt tttgaagata aggtttatgt
     6301 cagtggacaa atgaatctct tggattatga gccccgtcaa gatcttgatc agttcaaatc
     6361 gatgttttct tttatgaaag actcagacgg acttacacaa atgatcactc cgatggatag
     6421 caatattcat atcaagattg gtgctgaatt aggcaatgat ttattgcaga atatgagtat
     6481 gattcaagca agttacgaga tagatggtca tggaagaggt acaatagcct tgttagggcc
     6541 tactagtatg ccttattctc ggatgttcgg tctagtcgat gtttatcgaa gagaactagc
     6601 agctaaactt gctgattact accgttcgct tgacttgtca aactcgtaat gtgaataacg
     6661 aagctatttg cagctgatcg ttctctcttt cttagtaaga gcaatcaaac agctgactga
     6721 ataatgaaag gaagactgta tcgtgaaaga aaatcaagaa gaactagata aagaaatgac
     6781 agatgctcag ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg gtatttcaga
     6841 agctgaagcg gaagaattcg aaacagcgaa attaaaagct gagttagaag aaatggaaga
     6901 taaatacttg cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa aaaatgagcg
     6961 tgagcaatta caaaaatacc gttcacaaga tttagccaaa aaattacttc catcaattga
     7021 caacctggaa cgtgcattag ccacagaagt gtcagatgat caaggggcag gtttgaaaaa
     7081 aggcgtggaa atggtcttgg aaagtttgcg taacgcactg gaagaagaag gaattgaaaa
     7141 aatccctgct aaaggagaag cctttgatcc taatcttcat caagcagtac aaactgttcc
     7201 agcgactgaa gatacaccag ccgatacgat tgtagaagta ctacaagaag gctacaaatt
     7261 acatgatcgc gtgttaagac caaccatggt cattgttgca caataattgt gcggatataa
     7321 aacttacact ttcaattaaa gaaggagacg ttaattatga gtaaaattat cggtattgac
     7381 ttaggtacga ctaactctgc agtagcagta ttggaaggaa acgaagcaaa aatcatcgct
     7441 aacccagaag gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg agaaatccaa
     7501 gtaggggaag ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc atcaatcaaa
     7561 cgccatatcg gtgaagcagg ttacaaagta gatgtagaag gtaaatcata tacaccacaa
     7621 gaaatctcag caatgatcct tcaatatatc aaaggattcg cagaagacta tttaggtgaa
     7681 aaagtcgaaa aagcagttat cactgtgcca gcttacttta atgatgcaca acgtcaagca
     7741 acaaaagatg ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa tgaaccaact
     7801 gcagctgctc ttgcctatgg tttagataaa acagatcgcg acgaaaaaat cttagtattc
     7861 gaccttggtg gcggtacttt tgacgtatct atccttgaac taggagacgg tgtctttgat
     7921 gtattgtcaa cagctggcga caaccatcta ggtggggacg actttgataa caaaatcatc
     7981 gactacatgg tagcagaatt caaaaaagaa aacagcattg acttgtctca agacaaaatg
     8041 gctttacaac gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc tggcgtatca
     8101 agcacacaaa tcagcttgcc atttatcaca gcaggagaat ctggaccatt gcatttggaa
     8161 atgacattga cacgtgcaaa atttgatgaa ttaacagctg acttagtaga acgtacaaaa
     8221 gtaccagtac gtcaagcgct aaaagatgca ggtttatcac aatcagaaat cgacgaagtt
     8281 attttagtcg gtggatctac tcgtatccct gcagttgtag aagcagtacg taaagaaaca
     8341 ggaaaagaac caaacaaatc agttaaccct gatgaagtag ttgctatggg tgctgctatc
     8401 caaggtggcg tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt tacaccattg
     8461 tcattaggta tcgaaacaat gggtggagtg ttcactaaat tgattgaccg caatacaacg
     8521 attccaacaa gtaaatcaca agtattctca actgcggcag ataatcaacc agcagtagac
     8581 atccatgtat tacaaggtga acgcccaatg gctgctgaca acaaaactct aggaagattc
     8641 caattgacag atattccagc agctcctcgt ggtattccac aaatcgaagt aacattcgat
     8701 attgataaaa acgggattgt aaatgtatct gcgaaagatc taggtacaca aaaagaacaa
     8761 aaaatcacga tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg tatggtaaaa
     8821 gatgcagaag caaatgcaga agcagataaa gctcgtaaag aggaagttga cctacgtaat
     8881 gatgtagatg cattgctatt ctctgtggac aaaacattga aagaattaga aggcaaagtg
     8941 gacgaagaag aagtgaaaaa agcagaaaca gctcgcgatg aattgaaagc cgctgttgaa
     9001 gcaaacaaca tcgaagaaat gaaaactaaa cgcgatgcat tgaacgaaat cgttcaaaac
     9061 ttgactgtta aactatacga acaagcggca caacaacaag ctcaagaaaa cccagaagca
     9121 gctcaaggcg gagcagatga tgtcgttgat gctgattttg aagaagtcga tggcgacgat
     9181 aaataatcag ttcactgaat ttcgagagaa ggccaaagtt gctactttgg ctttttcttt
     9241 tgaaaaatag tttgaagtat cggtttttgt ttcaaacaaa atatgatatg ataacaagcg
     9301 acgtgaaaat gatgagaacc aataggagga aagcctatgg caacaaaacg tgattattat
     9361 gaagtcttag ggctctcaaa aggagcatca gaagatgaaa taaagaaagc ttatcgtaag
     9421 ctttcgaaaa aataccatcc tgacatcaat aaagaacctg atgcggaaga aaaattcaaa
     9481 gaagtatcag aagcatatga gattttaagt gacccgcaaa aacgcgctgc atatgaccaa
     9541 tacgggcatg caggcacaga tccaaattac ggtgctggtg gagctggtgg cggatttggt
     9601 ggattcggcg gcttctctgg tggcggcttc ggtggttttg aagatatctt cgactctttc
     9661 tttggaggag gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc tgatttgcaa
     9721 tatacggtcg acttgtcttt tgaagaagcg atttttggtg tagaaaaaga aatcaagtat
     9781 aatcgggaag aaatctgtca tacttgtggt gggaacggtg caaaaccagg cacacaacca
     9841 actacttgtc ataaatgtca tggtgcaggt acgattaatg tagaacgcca aacaccactt
     9901 ggacgagtga tgagccgtca aacttgtgat gtttgtcatg ggacagggaa agaaatcaaa
     9961 gaaccttgtc caacctgtca tggctcagga catgagaaga aagcacattc agttaaagtg
    10021 aatgtaccag ctggtgtaga agaaggacaa caaatgcgtt tagctggtca aggagaagct
    10081 ggagaaaacg gcggaccatt tggtgatttg tatgtggtct tccgtgtaga agaaagtgat
    10141 atctttgatc gtgaaggttc tgaaatttac tatgaattgc ctttaagttt tgtacaagca
    10201 gctttggggg atgaagtcca agtaccaact gtccatggag atgtgaaatt gaagatccct
    10261 gctggaacac agacaggtac gaacttccgt ttacgtggaa aaggcgctcc gaagttacgt
    10321 ggtggtgcaa caggcgacca acatgtcaaa gtaaaactga tcacaccgaa aaatctaaat
    10381 gaacaacaaa gagaagcatt acgtgcattc gctgaagctg gcggcatcaa agttgaagaa
    10441 cagcaagaag acggtttctt cgataagttt aaagatgctt ttggcgggaa aaagcggaag
    10501 tagtttttct agtaaaaacg ctaagtcatt aataattaaa aaccagacga acaatcatat
    10561 gttcgcctgg tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca gctttagaat
    10621 atgataatcg tcatagtttc taggggtata accagtgaga gaatggaact tgttcaatcg
    10681 atattgcaag gtgtttttat gaataaataa ttcttctgag caatttttga tgcttccgtt
    10741 atttttactg aagcatgcca aagtcttctt gaacaaatgt acttcttctt ctgtcaggct
    10801 tttcaaaata cgttttacat ataatgttcg ttgatctgac gggattgctg gtaaaacgat
    10861 cccgaggtct aaatcttcaa aaaagcggat ctcgttcaca gattgatgga aagctatcca
    10921 ttcgcaaact tgtttggcat gaaggaaaga ttgcggtgtc tcttgctctg tataacaaag
    10981 cgacccaatt ccaaagttta gctgtaactg atacatttta ttaaactttg tctggatttt
    11041 atcaagcaat gatagcaatg aggctctgga aatccgattg actaggataa tgatttgttg
    11101 tctatgtaca gtgagcaaag cctgtccgta ataagagaag accctttcga gcaactccaa
    11161 taaaggattg atccgttctt gacacaattg ctgattgtca gaaagttgtc ccacaactac
    11221 ataatatgga gaagaaaaat caagctcaat taagtgaggg tggtatcgac tatgtacttg
    11281 atatcatcaa tgaaccgatg gtactttctt atgcgatgga acaggtagag cgattgcttg
    11341 tttgtgcagg aaaacaagcg atgcggctga tacgattagg tgagaaaata agaagataaa
    11401 aatacacggt cccttcatgg cgaccgtgca tttttggttt ttattaaata tttaatactt
    11461 tattcaagaa atcttttgtc cgttcatttt gcggattttc aaagatttgt tgaggcgttc
    11521 catcttctaa aaagtttccg tcgtcaatga acatgacgcg attggccact tctttagcga
    11581 accccatttc gtgcgtaacg ataaccatcg tcataccttg ttctgctaac tttttcatga
    11641 cgttcaagac atcaccgacc atttcaggat cgagtgcaga agtaggctca tcaaaaagca
    11701 tgatgtcagg gttcatcgct agagcacgag caatagccac acgctgtttt tgtccaccag
    11761 aaagcatttc aggatacata tcttttttat cttctagacc aactgtctgc agtaatttga
    11821 ttgctttttt ctctgcttct tctctagatt ctttcttcag gtccatcggt gctaaaatga
    11881 tgttttccaa aatagaaaga tgagggaata ggttaaagtg ttggaatacc attccgatat
    11941 gttgtctcac caagttgata tctgtgcttt tatccatcaa gtgggcgccg tcaataataa
    12001 tttcaccgtc agaaggttct tctaaacgat tcaagcaacg caagaatgta ctttttcctg
    12061 aacctgaagg gccaatgata cagacgacat ctccttcgtt gatggataca ttgatgtcgt
    12121 ttaatactgt gttatcgccg tattttttga ctaaatgctc aactaagatt ttttctgcca
    12181 tcttacttca ccttcttttc taaaactttt gctaattttg tcaatgctgt gatcaaaatc
    12241 aagtacatga tcgcaatgat caagtaaaca tacgtacttt gtgttgtacg tgcaacaatg
    12301 atcttaccag tttgcagcaa ttcgactaca ccgattgcgg aaatgatcgt tgtgtctttt
    12361 aatgagataa cgaactgatt gacgaatgat gggatcatga tgcgtatcgc ttgcggcaaa
    12421 atgatttttt gcattgtccg attataagaa agacctaaac ttctggaagc ttccatttgt
    12481 ccgacaggaa cggcgttgat cccgcctcga acaatctctg cgatataagc acttgcgtta
    12541 agagtcaagg tgataacacc agcaaggaag tcaggaatcg taaatccaac gataccaggc
    12601 aacccaaaga aaataaagaa cgcaagaacc atcattggaa ttcctcggat gatatcgaca
    12661 taaatactag caattgtgcg aagagcgcgt attggtgcta cactgaataa tccaaaaatc
    12721 acaccgatta ctatagccaa agcaaaggag atcaaagtta ggagaattgt tctccaaagt
    12781 cctttcaaca atgcttgata gttgttttgg ataagtccaa gaaatgttga ttcatcagca
    12841 gtggaagact cactggaagt ttctgaacca tccgctacat attctttaat aatcttatcg
    12901 tattcgcctg ttctttttaa ttctttcaat ccttcattga acatattaag aagctcagga
    12961 ttttgtcctt ttttaacggc aaatgcatat tttccgccag attctttttt gatcggtgtt
    13021 tccaattctt gtccttgttt gatcgcataa ccgattaccg gataatcatc catcattgca
    13081 tcgattgcgc caacttttaa cccgtcgtac aattgatcag cagcatcaaa atatttgata
    13141 gaatagccat attcagattc atggtcgctt aaaaattcag cactttcggt accaactttt
    13201 gctccgactg tttttccttt caaatcttca taagacttga tcgaagtatc tccttttttg
    13261 acagctaatt ggatcccgct atcaaagtaa gaatcagaaa aatcatagct tttctttcgt
    13321 tcatctgtta cagtcattcc ggcaatcatt ccgtctgctt gtcctgattc caaagcttgc
    13381 aaagcagcag agaaaccacg gtagtccatt cggatgttaa atccttgcag tttggcgatg
    13441 ttttccatca attttacatc gatgccttcg tactcgccct ttgcgttttg aaattcaaat
    13501 ggagcaaaag cagagtcact ggcaattacg tatacgtctt ttttgggctc tattttttgc
    13561 attgtctcgc ttgtgcttga ctctgccgtt gtagtgttgt cagcttgtgc aacatgacca
    13621 aacgcaaatg tcacaagtcc tgtcattaag aaaacaaatg agtgaaataa atgctttctt
    13681 ttcataatca acctcctaaa tttccaatat tttattatta taggcgaaaa gtgctgaatc
    13741 agacaagact gtaagctcga aaaaatgaaa aaaatgcgag tttctaacat tttatgttat
    13801 aaaacataac atgtataata agataaaaaa gtggcgaaag tatgttcggt gtggtataat
    13861 tttttagtaa ggaatgcctt tgctttcctt ttctttttat tggtcgtaaa atgaaaagga
    13921 atgaatgaaa aggaggacga agaatgattg agagagatac ctcccgacac tttgagcttg
    13981 tctcgaaata ccaaccggct ggtgatcagc ctgaagcgat taaccaatta gtggatggcg
    14041 tagtcggcgg gaaaaaagca caaatcttat taggtgcgac tggaacaggg aaaacatata
    14101 cgatttccaa tcttattgag aaagtgaata aaccaacact gattattgcc cataacaaaa
    14161 cattggctgg tcagctgtac ggggaattca aggaattttt ccctaacaat gctgttgaat
    14221 attttgtttc ttattatgat tattatcagc ctgaagctta tgtgccttca agcgatacgt
    14281 atatcgaaaa agattcaagt gtaaatgatg agatcgacaa attacgacat tcggctacta
    14341 gttcattatt agaaagaaat gatgtgatcg tcattgcatc cgtttcatgt atttttggat
    14401 taggctcgcc ttttgagtac caaaaacaag tagtttcgat tcgacaggga gcagaactag
    14461 atcgcaatca gttgattcgt gacttagtca gtatccaatt cgaaagaaat gatatagatt
    14521 ttcagcgcgg tcgttttcga gtgcgcggtg atgtcgtgga gatttttccg gcttcaagag
    14581 atgaacgtgc cttacgtgtg gagttttttg gagatgaggt cgaacgtatc cgagaagtca
    14641 atgcactgac aggtgaagtt ttaggagaaa cggaacacgt agcaatcttc ccggcgactc
    14701 actttgtgac gaatgatgaa catatggagc atgccgtagc gaatatcaaa gcggaattag
    14761 aacagcgttt aactgtcttg cgaaatgaaa acaaacttct ggaagctcaa cgtttagaac
    14821 agcgaaccaa ttacgatatt gaaatgatgt tagaaatggg ctacacgtcg ggaattgaaa
    14881 attattctcg gcatatggac ggacgtaaag aaggtgaacc gccttatacg ctattggatt
    14941 tctttcctga agattttttg atcgtggcgg acgaatcgca cgtaaccatg cctcaaatca
    15001 gaggaatgta taatggtgac cgagcgagaa aacagatgct agtagattac ggtttccgtt
    15061 tgccatctgc tttagacaat cggccacttc ggttagaaga gtttgaaaaa catgtcaatc
    15121 agatcattta tgtctctgcg acgccaggac catatgaaca tgaacaaaca gataccgtta
    15181 tccaacaaat catccgtccg acagggctgc ttgacccagt gatcgaagtt cgtccaatca
    15241 tgggacaaat cgatgatcta gtaggcgaaa taaatgaacg agtcgaaaaa gaccagcggg
    15301 tgtttgtaac gacattgacg aaaaaaatgg cagaagacct cacagattat ttcaaagaat
    15361 tagggatcaa agtcaaatat ttgcatagtg atatcaaaac gctggaacga acagaaatca
    15421 ttcgagatct tcgattaggt gaatttgatg tgttagttgg aatcaatttg cttcgtgaag
    15481 gattagacgt acctgaagtt tcccttgttg ctatcttgga tgcggataaa gaaggctttt
    15541 tacgtagtga acgttcctta gtccaaacga ttggacgagc tgctcggaat gaagaaggta
    15601 aagtaatcat gtacgcagac aaagtaacag attctatgcg tctagcgatg gatgaaacgt
    15661 caagacgtcg tacgattcag cagaagtaca atgaagaaca tgggatcgtt ccaaaaacga
    15721 tcattaaaga aatccgagat ttgatttcta ttacgaaaga atcggaagat gatacaaaag
    15781 aagcggtaca agtctcgtac gaagaaatga cgaaggaaga aaaagacacg ttattgatga
    15841 aattagaaaa agaaatgaaa gatgcggcaa aagctttgga ttttgaaaca gccgcgaatg
    15901 tgcgagatat gattttagaa ttaaaagctt caaagtagga ggacaacatg gctaacgata
    15961 aaattacgat tcatggtgca cgtgcccata acttgaaaaa tatagatgta acgattccac
    16021 gagataaatt cgtggtcgtt acaggtttgt ccggttctgg aaaaagttcc ctagcatttg
    16081 atacgctgta cgctgaaggg cagcggagat acgtagagag cctttctgct tatgctcgtc
    16141 agtttttagg acaaatggat aagccggacg tagatagcat cgacggattg agtccggcga
    16201 tttctattga tcagaaaaca acaagtaaaa atcctcggtc gacagtaggt actgtgacgg
    16261 aaatcaatga ctatttacgc ttgttgtttg cacgtgtagg acatccgatt tgtcccaacg
    16321 atcatatcga gattaccagt caatcagtag atcaaatggc agatcaagta atggctttgc
    16381 ctgagcgcac aaagatccaa gtgttggcac cgatcgtggt aaagaaaaaa ggacaacata
    16441 aaaaaatatt cgaacgaatc caaaaagagg gatacgtccg cgttcgagtc gatggtgaga
    16501 tctatgattt aagtgaagtg cctgagctgg agaaaaacaa aaaacatgat atcgcgatcg
    16561 tcatcgaccg gatcattgtg aaagaaggga ttcgttctcg cctatttgat tctttagagg
    16621 cggccctgcg attagcagac ggctatgctc tggttgacgt gatcggtgaa gaagaaatgt
    16681 tgtttagcga acactatgcc tgtccttatt gcggatttac tgttggcgaa ctagaaccaa
    16741 gactgttctc attcaatgcg ccttttggag cttgtccaga ctgtgatggt ttaggtgtca
    16801 aattagaagt cgatcaagat ttagtgattc cagatagaac gaagacactt agtcaaggtg
    16861 ctatcgttcc atggaatcca atcagctctc agtattatcc tcaaatgtta gcgcaggctt
    16921 gtgaaagttt cggtatcgat ttagatacac cattcgaaga attaccggaa gagcatcagg
    16981 agatcatctt gaatgggaca gatgatcttt tccatttcca ttatgaaaat gattttggtg
    17041 gcgttcgcga tgtagaaacg actttcgaag gggtattgaa gaatatcgag cgtcgttatc
    17101 atgatacgaa cagtgatttc acgcgtgagc aaatgcgtct ttatatgaca gaattaactt
    17161 gtcagacatg taaaggttat cgattgaatc cccaagctct atctgttcaa atcaatggaa
    17221 cgaatatcgg gcaaacaaat gagttgtcta ttcaaaaagc agtagatttc ttctcaaact
    17281 taacgttatc tgaacaagag aaagtaattg ccaaaccgat tttaaaagaa gtaaatgaca
    17341 gactttcatt cttggaaaat gtgggactag actatctaac tttgagtcgc gcttccggaa
    17401 cattatctgg gggagaagcg cagcgaatca gattggctac acaaattgga tcgaatcttt
    17461 ctggagtttt gtatatttta gatgaaccat cgatcggatt gcatcaacgc gacaacgatc
    17521 gactgattgg ctcattaaag aaaatgcgtg atcttggcaa caccctcgtt gtagtcgaac
    17581 atgatgaaga tacgatgtgg gcagcagatt atttgattga cgtcggacct ggggccggag
    17641 aacaaggcgg acaaatcgtg gctgcaggca ctcctgaaga agtagctaat gatgaaaatt
    17701 ctttgacagg gaaatatctt tcagggaaaa aatcaatccc tgtaccaaaa gaacgccgta
    17761 aaggtaatgg gaaagcaatc aagatcactg gtgcttcaga aaacaatctg aaaaatatta
    17821 gtgttgaatt tccattagga gaatttgtag cagtcacagg tgtgtctggt tctggaaaga
    17881 gtacattggt caatagtatc ttaaagaaat cgttagcgca aaaattaaat aagaattctg
    17941 ctaagccagg taaattcaag acaatttccg gctacgaaag tatcgaaaag atcatcgata
    18001 tcgatcaaag cccaatcggc cggacgccga gaagtaatcc agcgacttat acaagtgtat
    18061 ttgatgatat ccgtgggtta tttgctcaaa cgaacgaggc aaaaatgcgg ggttataaga
    18121 aagggcgttt tagtttcaac gtaaaaggcg gtcgttgtga agcttgtcgc ggggatggaa
    18181 ttattaagat cgaaatgcac tttttgcctg atgtctatgt tccttgtgaa gtatgtcatg
    18241 gtaaacgata taattctgaa acattagaag tgcattacaa aggaaaaagc attgctgata
    18301 ttttggaaat gacagtagag gatgctgtag aattcttcaa gcacattcca aagattcatc
    18361 gcaaactgca aacgattgtt gatgttggct taggttatgt gacgatgggg caaccagcaa
    18421 cgacattgtc cggtggtgag gcacaacgga tgaaacttgc cagtgaattg cacaaaatct
    18481 ctaatggaaa gaatttctat atactagatg aaccaacgac aggacttcat agcgatgaca
    18541 tcgcccgctt gttgcatgta ttacaaagat tagtagatgc tggtaacaca gttttagtga
    18601 ttgaacacaa tctagatgta atcaaaacag cagattatat cattgattta ggaccagaag
    18661 gtggagaagg tggaggaacg atccttgcga ctggaacacc agaagaaatc attaacgtaa
    18721 aagaaagtta tacaggtcac tatttgaaaa aaataatggt ataaagagga tagatagctg
    18781 agatagttaa aaaaacataa aaaaatgaat ttttgcttgt tttattaata tcagtgttct
    18841 agaatcatta catggtagtt ctttctacac tgattggtat agataaaaac ttcctataag
    18901 ttcaatccgt tcaaaaaaga attgagcaat cggaggactt tcctttgatt gcttttttct
    18961 ttatggtatg atattccttg tgtagttgga aaagaaaaac aagagattat gagtaagtga
    19021 gagataatga tgaacagtat tgaaaatgaa gaatttatcc ctatattatt aggaagcgac
    19081 atgaacgttt atggtatggc acgttctttt aatgaagcat acggcaaaat ctgccaagca
    19141 tatgcttcgg atcagttagc ccctacccgc tacagtaaaa ttgtcaatgt agaagttatt
    19201 ccaggatttg ataaagaccc tgtatttatc gagacgatgc ttcgtttggc aaaagaaaga
    19261 tattctgata agagtaaaaa atatcttttg attgcttgtg gtgatggata tgcagaattg
    19321 atctcccaac ataaacaaga gctgtcagaa tattttatct gtccatatat tgattattct
    19381 ttatttgaaa ggctgatcaa taaagtaagc ttttatgaag tttgtgaaga atatgatctt
    19441 ccgtatccta aaacattgat cgtcagagaa gaaatgttag taaatggaca tttggaacag
    19501 gaattgcctt ttgaattccc agttgctttg aaacctgcaa acagtgtgga gtatctttct
    19561 gtccaatttg aaggaagaaa aaaagctttt attttagaga ctagagaaga gttcgattta
    19621 attttaggcc ggatatacga agccggttat aaaagtgaaa tgattgtaca agattttatc
    19681 ccgggtgatg atagtaatat gcgtgtgcta aatgcgtatg tagatgaaga tcatcaagtt
    19741 cggatgatgt gccttggtca tccactatta gaagatccta caccagcatc gattggtaat
    19801 tacgttgtga tcatgccaga ttataacgaa aaaatctatc aaaccattaa ggcatttttg
    19861 gaaaaaatcg aatatacagg ctttgccaat ttcgatatga agtatgatcc tagagatgga
    19921 gaatataaat tatttgaaat caatttgaga caaggtcgta gcagtttctt tgtgacattg
    19981 aacggcttga atctggctcg ttttgtcaca gaagatcgag tgttcaacaa accctttgtt
    20041 gaaacgacct atggaacgaa tcaatcagat aaagcaagat tatggatggg tgtgcccaaa
    20101 aaaatctttt tagaatatgc tcgggaaaat gaagacaaaa aactagctga gcaaatgatc
    20161 aaagaaaatc gttacggtac tactgtattt tatgaaaaag accgatcaat caagcgctgg
    20221 ttgttgatga aatatatgtt ccataactat attccaagat tcaaaaagta ttttcatgtg
    20281 aaagaaggct agttcatgga gaattttttc agtattttag gcggaatggg cacgatggcg
    20341 acagaaagtt ttgttcgctt gatcaaccac cgaacgaaag ctacaaaaga tcaagaatat
    20401 ttgaattatg tcttattcaa tcatgcaaca gttcctgatc gtacagctta tattttagat
    20461 cgatcagaag aaaatccaat gccgtttctg ctggatgata ttgagaaaca aaatttattg
    20521 cgaccaaact tcattgtgtt gacttgtaac accgcacact acttttttga agagttacaa
    20581 gcggcaacgg atattcctat cttgcatatg ccaagagaag cagcaaatga acttgtccgt
    20641 cagcatacga cagggagagt agcgattcta ggaacagaag gaagtatgaa agctggaatc
    20701 tatgaacgcg aagtcaaaaa tcttggtttc gaaacagtga ttcctgatac tgcgcttcaa
    20761 gaaaaaatca attacctgat ctatcatgag atcaaggaat cggattattt gaatcaagag
    20821 ctctattacg aaatcttaga ggaagcggta gagcgcctaa actgtgaaaa agtcatccta
    20881 ggttgcacag aattatcttt gatgcatgaa tttgctgaag ataatcatta cccagtgatc
    20941 gatgctcaat ctattttagc ggatcgaacg atcgaacgtg cattagctga aagaagcgag
    21001 gcattggata cagcatcgga aaagtaaaaa aattggattt ggggcaaata tcctaaaaga
    21061 tatttggtcc aaatcttttt gtaaaagaaa aagaaaactg gtaatgatac gcccgggatg
    21121 atataatttt attatgcaca atgtgagagg ggttcgtaaa tatgacagat aatttgcaat
    21181 tagtcatcat aacaggtatg agtggtgcag ggaaaacagt agctatccaa agttttgaag
    21241 atatgggcta tttttgtatc gataatatgc cgcctcgatt gattccaaaa ttctgggaat
    21301 tgatcaaaga atcaggtaaa gtcacaaaaa tggctttagg agtggatttg cgttctcgtt
    21361 ccttctttga agaaatccaa aacatgctga tcgaaatcga aaatacgaac ttcatcgaca
    21421 cttctatctt atttttagat gcttcagacg aagaactagt ggctcgctac aaagaaacaa
    21481 gacgagcaca tccaatggcg atggacggtt tgatcactga agggatacgt aaagaacggg
    21541 cgatactaga cgacttgaaa gcccaagctt ccgtgatcat tgatacgaca acattgacac
    21601 cacgacaatt acgtgaaaaa atcaatgagt cattcaaatc aaagggagac caaggatttc
    21661 ggatcgaatt tgtatcattt ggttttaaat acgggttgcc gatcgattcg gatattgtga
    21721 tggatgtgcg ttttttgccc aaccctcatt atatcccgga actaagacca ttgaccggaa
    21781 aagatcagcc agtctatgat tatgtgatgt cctttgatga aacgcaatca ttctaccata
    21841 aatttttgga tcttctggaa actgtattgc ctggatacat taaagaagga aaaagcagct
    21901 tgacagttgc aatcggctgt acaggcggtc aacaccgttc tgtggcattg actgaacgtt
    21961 tggcaaatgc tttagggaaa aactataaag tcaatgtgac acatcgggac aaggataaaa
    22021 ggaaagagac ggtgaaccgg tcgtgaaaat gaaaacctac cggataagaa aaccaaagat
    22081 cgtagtggtc ggcggtggca ctggtctgcc agttatcctg aaaagtttac gaaatcaagg
    22141 agcagatatc acagcagtag tgactgttgc agatgatggt ggaagcagcg gtgcaatcag
    22201 agaatcgatc gctatggctc ctcctggtga cttgagaaat gtcttagtgg cgttatcgga
    22261 tatgccacaa ttctatgaag atattttcca ataccgtttc aaaaaggaag atcagttttt
    22321 agccaatcat acgatcggta atctgatcat tgccgcagtt tccgagatgc ggggaagcac
    22381 ttatgaggcg attcaattat tagcaaaaat gatgcatgta gatggacatg tttatccttc
    22441 ttcagaaact cctttgacct tgcatgctgt ttttaaagac ggaacgactg cagtaggaga
    22501 atctaaaatc gcgatcaacc gtaaaaccat tgaccgtgtg tatgtctgca acacaaatga
    22561 taaaagtgaa gccaaagcgg ctcgaaaagt cgtttcagca attatggacg ccgacatggt
    22621 cgttttggga cccggaagtt tattcaccag tatccttcca aatttagtga taccagaaat
    22681 cggcgaagca atgctcaaga caactgctga aaccgtctat atctgtaata tcatgaccca
    22741 aaaaggggaa acggaacatt tcacagatgc cgatcatata cgtgttttac acaagcattt
    22801 gaatcagcct tttatcgata cagtattggt caatacggaa aaagtgcctg aagattatat
    22861 ggacccagag atctatgatg agtatctagt acaagtcgca cacgacttca acggattgag
    22921 aaatgaagga tgtcgagtga tttccaccga ctttttagaa ttgaaagatg gtggtgtctt
    22981 ccacgatgga gacaaagtag tagaagaatt gttccgttta gttttcggtt caaaatatta
    23041 agcaatttat tgttggagaa aggaggagaa taggatgtca tttgctgcag atgtgaagaa
    23101 agaactaact ggtttggaag ttcatcgaga acatgcgaaa gcggaattag ctgctttgct
    23161 gcgaatgaac ggctctttag gtattgtgaa ccagcagttt attttgaacg tccaaacaga
    23221 aaatgctgca attgccagac gtatctattc tcttttgaaa gatcattatc aggtacgaag
    23281 tgagctactc gttcgtaaaa aaatgaaatt aaaaaagaac aatgtctata tcgtccggtt
    23341 gaagcaaggc acaaaagatg tgttgatgga tcttgatatc atggatggta tgatgttcaa
    23401 tggacacgtt tcggacgaga ttatgggaaa ttcgcaaaaa atgcgttctt acttacgtgg
    23461 tgcttttatg gcctcaggtt cagtcaataa tccggaaacg agtcgttatc atttggaaat
    23521 attttctatt tatgaacagc ataaccaaga catttgcgat atgttaaatt attacggtct
    23581 acatgctcga acgcttgaac ggcgcaatgg ctatatttcg tatttaaaag gagcagaaaa
    23641 aattgctgat ttcttaacat tgattggtgc aaccaactca atgctccgat ttgaagatgt
    23701 tcgaatcgta cgggatatga gaaattcggt caatcgtcta gtcaattgtg agacagcaaa
    23761 tctgaacaag acgattgatg cggcttctaa acaaatcgaa aacatcgaat tgatcgaagc
    23821 aaaggtcggt ctacatgccc ttcctgaaaa acttcaggaa atcgcagaac ttcggcttca
    23881 acatccagag gtcagcttga aagaattagg ggaaatgatt ccctcaggtg caatatcaaa
    23941 atcgggaatt aatcaccgaa ttcgcaaaat aaatgaattt gcggaaaaac tgcgtgaaga
    24001 agcagtttaa tccaaagaaa aaactgatac atgaagcagg agatgcgaat gtatcagttt
    24061 tttttaaaag ctatacttgc ttttcttccg tcaaactata attccaactt tactggagta
    24121 acataatgtt gaaaggcttc tattgttttt taatacaatc gtataaaaag cggggagaca
    24181 actgaatgaa gattttagtt ttatacataa acttttgctt aatatagaag tcctgatata
    24241 gataggatca agtatttatc tataactgga gattaattaa aaactggaag gaactgctcg
    24301 atcaaccttg agaactgttt ttcttacttc tggtcaagat tctcgtggtt ggtggataag
    24361 gatggttgat aagttcattg gattagctag tcgaaaacaa ccaggtgtaa ttaatgtaga
    24421 agttttaaca aggggatgga gtgatttatt cagtgataat cataagttca taaatagtaa
    24481 ttgggatgta tggttggtat acgttatacc tgaacttgaa ctatcaagat ttggtttaga
    24541 atttgagaat cgatgtttat aaagggaggg caaaaaatgt gcttttttac aacttttact
    24601 atcattatga ttgcagtact ttttattttt ctagatatag caaagcgaaa tacagcattt
    24661 ttattatatc gggttttact tcgagcaggt ctcataacat ttatatcgat cgttggattt
    24721 tttctattca cagttattgt atttatttgg agaactacag caccaccatt gccagagatt
    24781 acatatggag aatttccgtt tagtcttgaa tatgaaatag atgaggaaag aattttgatt
    24841 gaagatacgg tgattgcaga atttatagga agttttagag ggaatgtgac aactcaacct
    24901 agacgaagtt ggagtataaa acttttaaat aatagtggag agttgaaagc atttcattat
    24961 gaattcttaa taatagaaag tgaagatgta acaatcactt taagtagtgg ctctgctgga
    25021 tattttatga atgatggtgg aagcaagcca cagctcacag tccgtgatcg tattgcaggt
    25081 tttatgcatt tttctcacta tgacttagaa gaagctcgta aaatacttgc agaatatggg
    25141 atcacattaa tcaattggga atatgcgcca ccgataagaa attttttcaa tgattattat
    25201 tagtcttata aaaaacagaa tgtaaaattt atgtgcgcga gtatttagtt ttccattttt
    25261 tgtttgatag atatatagca ttgttgaagc atgggatagt ggtgccaatt ttatcaatga
    25321 ggttggtcag gcagttaaca taattggtga tgctataaat ccatttaatt ggaattggag
    25381 gaattaggtg tggaaacagt gagattatgg atattcatga aaaattttcg ttcggctttg
    25441 agatatgtta ttctggaatc aaaatcaggt tattatctga ttgacacaga tcactatact
    25501 ttttggggag gctacctttt tccgattttt aattggataa tcccccataa ggttatgaaa
    25561 attaacttgc ctgcagaaga aataaataga ttaatactca acaaagattt acaaaggaaa
    25621 agagagaaag aacattctaa tgttgcttgg ataggtccag catttgcagt agtgacttct
    25681 tcaattgtcg gtcctatgct agagagtatt tatagtgatt tttctattgg aatacgttat
    25741 ttatttgtta ctttaagtat agctgttctt ttgtgcataa agggttatgt tagcaagaag
    25801 aataaagata ttttaacact aatcggtgta aacaatttat ctagtactaa aattatactg
    25861 tttcctgaat ctataaaaga aggatcaagg ttgttgataa tttttataat gcttatactg
    25921 ttttctgttt tatttggggt
//
LOCUS       DACDSY010000020        25467 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDSY010000020 DACDSY010000000
VERSION     DACDSY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.020"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(186..875)
                     /locus_tag="IWK44_000751"
     CDS             complement(186..875)
                     /locus_tag="IWK44_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9549303.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(920..1684)
                     /locus_tag="IWK44_000752"
     CDS             complement(920..1684)
                     /locus_tag="IWK44_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP9549304.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(1712..2410)
                     /locus_tag="IWK44_000753"
     CDS             complement(1712..2410)
                     /locus_tag="IWK44_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP9549305.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(2423..3202)
                     /locus_tag="IWK44_000754"
     CDS             complement(2423..3202)
                     /locus_tag="IWK44_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP9549306.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            complement(3218..4162)
                     /locus_tag="IWK44_000755"
     CDS             complement(3218..4162)
                     /locus_tag="IWK44_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9549307.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4203..4982)
                     /locus_tag="IWK44_000756"
     CDS             complement(4203..4982)
                     /locus_tag="IWK44_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549308.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            complement(5366..7441)
                     /locus_tag="IWK44_000757"
     CDS             complement(5366..7441)
                     /locus_tag="IWK44_000757"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP9549309.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
     gene            complement(7443..8360)
                     /gene="glyQ"
                     /locus_tag="IWK44_000758"
     CDS             complement(7443..8360)
                     /gene="glyQ"
                     /locus_tag="IWK44_000758"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP9549310.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(8744..9556)
                     /gene="recO"
                     /locus_tag="IWK44_000759"
     CDS             complement(8744..9556)
                     /gene="recO"
                     /locus_tag="IWK44_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP9549311.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(9699..10598)
                     /locus_tag="IWK44_000760"
     CDS             complement(9699..10598)
                     /locus_tag="IWK44_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP9549312.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(10613..11014)
                     /locus_tag="IWK44_000761"
     CDS             complement(10613..11014)
                     /locus_tag="IWK44_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP9549313.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(10992..11468)
                     /gene="ybeY"
                     /locus_tag="IWK44_000762"
     CDS             complement(10992..11468)
                     /gene="ybeY"
                     /locus_tag="IWK44_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP9549314.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(11485..13677)
                     /locus_tag="IWK44_000763"
     CDS             complement(11485..13677)
                     /locus_tag="IWK44_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP9549315.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            complement(13701..14672)
                     /locus_tag="IWK44_000764"
     CDS             complement(13701..14672)
                     /locus_tag="IWK44_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP9549316.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            15378..16757
                     /gene="brnQ"
                     /locus_tag="IWK44_000765"
     CDS             15378..16757
                     /gene="brnQ"
                     /locus_tag="IWK44_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9549317.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(16911..17357)
                     /locus_tag="IWK44_000766"
     CDS             complement(16911..17357)
                     /locus_tag="IWK44_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP9549318.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(17384..17560)
                     /locus_tag="IWK44_000767"
     CDS             complement(17384..17560)
                     /locus_tag="IWK44_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP9549319.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(17722..18150)
                     /locus_tag="IWK44_000768"
     CDS             complement(17722..18150)
                     /locus_tag="IWK44_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9549320.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            18242..19102
                     /locus_tag="IWK44_000769"
     CDS             18242..19102
                     /locus_tag="IWK44_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP9549321.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            complement(19194..19487)
                     /locus_tag="IWK44_000770"
     CDS             complement(19194..19487)
                     /locus_tag="IWK44_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9549322.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(19559..21286)
                     /locus_tag="IWK44_000771"
     CDS             complement(19559..21286)
                     /locus_tag="IWK44_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9549323.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EENHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(21500..22426)
                     /gene="trxB"
                     /locus_tag="IWK44_000772"
     CDS             complement(21500..22426)
                     /gene="trxB"
                     /locus_tag="IWK44_000772"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP9549324.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(22571..24004)
                     /gene="ascB"
                     /locus_tag="IWK44_000773"
     CDS             complement(22571..24004)
                     /gene="ascB"
                     /locus_tag="IWK44_000773"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9549325.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(24004..25407)
                     /locus_tag="IWK44_000774"
     CDS             complement(24004..25407)
                     /locus_tag="IWK44_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9549326.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
BASE COUNT         6863 a         5461 c         4508 g         8635 t
ORIGIN      
        1 catcccaaaa tttcgaatat tttcttttat ataagaataa tgatactgaa ttgccccatt
       61 cacctttctt tttttagatt ttctacaagt gattcaaacc ctccataatt acagttatta
      121 cttttgaaac gataatatga acatgtgtaa tcttttttat taacaatcca tcattctatc
      181 cctaactaat atgctccatt gggcttgaca ataacataaa acgtttttaa aatcaacctc
      241 aagtcgtaca ttaaacttcg atgactaata tactctaaat ccaactcaac cattttatgg
      301 aaactaacat tatttctatc actaacttgc cacaaacctg aacaaccagg ttttacatac
      361 aagcgttgaa gatcatatct cgtgtactca gctacttccc tctccaaagg tggccgtgga
      421 ccaattagac tcatatcgcc ttttaaaaca ttccatagct gtggcaattc atcaatgctt
      481 ttggcacgga taattttacc tattttagtg acacggggat cttctttcat cttgaacatt
      541 gcaccttcta tttcattttg atcaagtaat tcttccaatt tttcttcagc atctaaacac
      601 atcgaacgga atttatacat tttgaatgtt tttccatttt taccaacacg tgtatggcaa
      661 aaaaatatcg gacttcctgg ctcttctttt ctgattcgat aagctacata gaacatcaaa
      721 ggagaagcta aaatcaaacc aataatgctt cctacaatat caatcattct tttaaaaaat
      781 aaatagatat atcgtttttc aactttttct ttatccaacc aaacttgatt aggatgtact
      841 tcactaatac gagataataa atcttcactt tccatacttt ccctctctcc tcatttcctt
      901 tttatctacg aataaagaac taaaataaac caaacttctt cttttttacc tcttcataga
      961 ctggataact gatttcatcc ccgttgatca gatcctttgc gacttgtttc atctgtacta
     1021 ctttttcctt accaaagtcc ttagcaatct gctcatagca ctcttttaag taaaaggtac
     1081 gcttagagac cccatgggca tcagaagcag ccatctgcac aagattatgt ttgaccatca
     1141 ttttggctgt tttctggatg tcttttccaa atacaccgac ataacttggt gcggtgagtt
     1201 gggccaagca tcccatatct aggaatggca gcatatgatt cgggtcttta cgaaaatgcg
     1261 cgttgcgttc tggatggacg atcactggtg tctttcctag ttgtaacaac tcaaaaaaga
     1321 gctgttctgc atataacggc acttccagtg tcggaaattc gattagaaga tatgtatcat
     1381 ctaaatctgt aaataaaatt tcatctcggt cgatgtcttc gatcagtgtt ccggtaatac
     1441 gtacttcttg tccttccaat aacgtcaaag gcaattgtct tttctctaat tctgcttgta
     1501 acgaagcgac tagagaaatg acttgtgatt tttcattgct gtattttcca ttattatgat
     1561 ggggtgtgca taaaatatgt gtgatccctt gttgaatcgc tttttcagcc atggcgatac
     1621 tggcatctat gttttctgct ccgtcatcga ttcctggcag gatatggcaa tgtagatcga
     1681 tcatttctct tgtctcctct ctttttattt cttaattccc gtagtaatag ccagcatctt
     1741 tgctgtgttc tgccccattg tatacaacac caaggactcg cgcttgtacc atatccagca
     1801 attcttttgc tttagtcaat gattctttcc gtgacatatt ttcacgtacg accaataagg
     1861 tgccatccgt tttggaagac atgatctgtg catctgttac ggcaactact ggcggcatgt
     1921 cgaagatcac tacatcaaat agttgtcttg cttctgctaa aatctgatcc attcttggcg
     1981 aacttagtag ttctgatgga tttggtggtt taggtccact tggcagaatc gacaaattct
     2041 cgactggtgt tcgttggatc tcatctgcta cacttcctga tgaactaaga acggtactta
     2101 atccgcttgc gttgttcaac ttgaacgttt tatagatgac gggcttacgt aagtctgcat
     2161 cgactagcag cacccgttga ccagatttgg caaaaacaac ggcaatgttt gctgcagtgg
     2221 tcgatttccc ttctccaggt cctgatgaag tcacaacaat cgttttaatt tgctgaccag
     2281 ctgcggaggc aaactggata ttcgtccgaa tcgtacgata ttgttcagcg attggtgaag
     2341 aggggtttgt gagtgtgacc aaactgactg catgagtttg ggctgctttt tgtttctgtg
     2401 ttcgtgccat ttttctttcc tcctagactc ttgagcggct tctgcgtgat ggcgctgttt
     2461 tatcgtttgt agcttttggt tgtgtcggag ctggtccttt tttacggata ccagcattca
     2521 attctttcgc tgacatttct ggtacagtcc ctaagatcgg gaagcccaat gtatccgtca
     2581 catatttttc atcttttact gtccggtcga gtaattcaaa taagaaagct agacctacgc
     2641 ctaccatcat tcctaacact aagccaatca ctagattcaa tttattattt ggcgaaacag
     2701 gtgtcgtatt ggcaactgca cctgagatga tcgagatttt atcgacattc atcacgtctt
     2761 tggcgttctc ttggaatact tgtgacgttg tattagcgat ttgttgagcc gtcactgcat
     2821 ttgtgcttgt tgcttggatc gagaacattt gtgaattttg tgactgattg acggagatgg
     2881 catctttgat ttgtccagca gtcattttta cattgtcttc tgtttctaaa cgatctttga
     2941 cttcatttag tactagatca ctgacgatca tatccttata cgtattgatc atttgcaggt
     3001 tcgtattgac atcattgaca tttgcagtat ctgattgcgg cagtgtcacg atcaattggg
     3061 cttgtgagct gtattttggc gtcaagatga aaaacgtcac gatgcctgag atggccaagc
     3121 cagcaaacat tgagatgagg attgttatca cgtgtttgcg taatgtttca aaaatttctt
     3181 tcaagctgat tgtctcttcc attttttctc cactttccta tttattctct agattcattt
     3241 gatcttttaa ttcttttttc acacgatcaa gttcttgatc gctgactttt tggtatgaga
     3301 tattgtcttg catgaagcct tctccttgca gctgatcttg cttgatcgta ccaaatgcat
     3361 cccgataatc taaagcaatc tttttcattt catcaaagct catgtctgtt ttcatattcg
     3421 actctagagc attcaaaacg gattgatagt tgcttacacc gttcagactc aacacttttt
     3481 tcgctacacc ttctacgatc tttctttgac gttcttgtct gccgtagtca ccatttggat
     3541 cttcataacg catacgagaa taagacaatg cctgatcccc gttcaatgtg attttaccga
     3601 tcgtaaaatc atagccatct tgtgagaagg tgaggtcatt attgacttcg ataccaccaa
     3661 ctgcatcaac gagttctttt agacctttca tattgattga aacatagtga tcgattggga
     3721 tgtctacata tttttgtacg gtatccatcg ccatggcagc tccgccgaat gcgtaagcgt
     3781 ggttgatctt gtcttttgtg tcatgtccaa cgatgtcgac atacgtatca cgaggaatgc
     3841 tgacaatcgt tgtctgtttg tcttcaggac tgactgtggc aaccatcatc gtatctgatc
     3901 ttccttgatc gacccgacct aaatctcccg tatcaatccc catcaataga acagagaaag
     3961 attcttgttt gtccagatca actggttttg ttcgttctcc ttgggttcgt tccactgatt
     4021 cgtaagtttt ttcaatagaa tgtgagagat ctagatataa ttttgctcct actcctacga
     4081 ttgctaatac taatacgaca agaattgcta aaataattag aaatatcttt ttccctcttg
     4141 atcttcttct tcgttttgcc atagtttcct ccgtttgcta gccttgcttg aattctcgct
     4201 tattaatagc tcgtattgct atagcccatt tgctgagcga ctgtttctgc ttttgacgcg
     4261 tatgcggctc ttattttttc attccggatc tgttcgatga gactgagtgc ggtcaaatat
     4321 tgatcgtaag tagcagtagc tgttacttga ccgtctttca gcatcgtatc gaatagctga
     4381 tctagttgcg tgacttgtgc aatttgggta tcagcgaatc ctagatattg cataacgacc
     4441 gttttccaag cactatcctt aaaaaagctc atattttcac gtacatgggc gacatctgca
     4501 cttgccaact tttctttgat gatgacatca tccacagctt gctgccagtt tgatacgttt
     4561 ttagtaaata atttattgac agccgtttgg atcttcagtt ttgattctgc atcctctaac
     4621 tgtgttaaga ctgctttctt ttcttcttgg atcgcagcag cttctttagg taattctgat
     4681 tccttgatgt cataatcatc tgctgaaagt ttgatagaag atagtttatg tgacaaattt
     4741 gaaactgttt cttctgttat ttcaggattt aggaaaattt tttcttttgt actgtacagc
     4801 ttatctactt cttttgataa agaggtcacg gtgtcttttt gattggtgat ggcagattct
     4861 gcgtaattga tccgttgatg atgttgccgc tgattattga gatggtatcc aaacaagcaa
     4921 atcacaatag cgcctgtgcc taccgccaca gtagccaata acttgacgaa cggcttttcc
     4981 atagattcac tcctaaatat tgatgataga taaataaaga tgtgattcat tcacatcaga
     5041 atgagcgctt tttattttaa aagcataatt aaactaatat attcatagaa atatttataa
     5101 acaggttttc cctatataat cataccacaa acagttttct tgactaggga aattcctaat
     5161 ttaaaagtaa ataaaacagt aacaagatta ttattaatct ttttcatttt tatttcctca
     5221 cttttcatga gcgttttgaa aattctagca aagaataaaa taacaaaaat tattaaaata
     5281 gaattaataa cagcttttat atttataaca aaaaaacaga gacacagcac gtttcgtgca
     5341 tgctctctgt ttttcaaaca aaagactatt ttgtgatgat ttgatccaag cttgctaatg
     5401 acaaagccat ggcagttatt tgactaagtt gtgttagtcg gttcttacgt actgctggtt
     5461 catcagccat gaccatcgtt tgatcaaaat aattttcgat cagtggacga agagcgacaa
     5521 gctggtcgta gttttcgtgg atcgtttggt cagcaaaact tgctttcatt gcttctactg
     5581 ctttgtgtaa ttctttctct gcgtcgtttt cgaacagctc agggtctact tctgattgtt
     5641 tttcaagctg cgcttttttc gctaagttca cgacacgtgt caatgcttcc attgaaggtt
     5701 tgaagtcttc atctgcaaga tgttgtttga acaattgtgc tgctgcaaat acttttgtca
     5761 gatctttttg ttctgcattc aaaacagctt cgatgacatc atgacggata ttcttcgttg
     5821 tcaataattg gcgcagacgg cctttgataa agtcgatcac ctgttgttgt cccgctgaca
     5881 agccaatgcc aaaacgatcc gtatctttac tgataacttc actaatcgtt tcttgcagaa
     5941 cagacaaagg aaaggcccat tttttcgatt cgatgattcg aacgacacca tatgcttgac
     6001 ggcgcaaagc atatggatcg tttgagccac ttgggatcat cccaactgca aagaaagaaa
     6061 agatactatc taatttatca gcaatcgcta aaacagaacc tactgtagtc tcaggtaaat
     6121 ctccttcact ggatataggc atgtaatgtt cacggatcgc tgttgctacg gctggtttct
     6181 cgcctttaag cagagcatat ttttctccca tgatcccttg tagttcaggg aattctccta
     6241 ccatgttggt tactagatca aatttataga tttcagcagc gcgttgcaga tccatcaatt
     6301 cttcctcaga tagtcctact tgttcgccta tgatgcgagc gatgacttgt acccgttgca
     6361 ttttctcata gatgctaccg attttttcat ggaaagtaac agtttttaat ttttcaacgt
     6421 attggtcgat tgaagctttc ttgtcttcat tataaaagaa ttccgcatct tctaaacgag
     6481 cagtcaatac tttttgattg cctcgtacca cattttccaa ttttacccga tcgccattac
     6541 gagcagaaat aaaatgagga agcaacatac cttgatcgtt ccgtacttcg aaataacgtt
     6601 gatgttcttt catagaagtg actagtactt cttcaggaac agatagatat ttttcttcga
     6661 agtcgccaga aaagacagtt ggatattcga ctagattgtt gacttcttct agtaaatctt
     6721 catctagatc aatgatccag tgattttttt ctgctaattc gtttgcttgt gtgatgatca
     6781 tttcttttct tttttgcggt gaagcaataa caaattgttc attcaatttt tcttcgtatt
     6841 cttttgcatg cgtaaatgtc acatcttcgc ctaagaaacg atgacctctt gaaactctgt
     6901 ctgttttgac atccaagatc tcaaacggga ttatttcgtc atctaacaat gccacgatcc
     6961 agtgaatagg gcgaatatat tcaaaatcgt attctgccca gtgcatcgtt actgggaaag
     7021 tgaggctttt aatgacatct gctaaacctg tcaatacttc ttttgcttct ttgccgtgag
     7081 tgtatttcgt tacatagaca tattccacgc cttttaattc tctaaaagtg atagcatctg
     7141 tcgtcagacc ttgtccgcgg acaaatcctt cagctgcttt cgtccagttt ccatctgcgt
     7201 ccaaagcaat ttttttggct ggtcctttga cttcttcttc gctgtctgct tgacgttctg
     7261 gaatgtttgt tactttgatt gctaagcggc gcggtgtaga aaacgtttcg atcgtatcat
     7321 aagtcaattg gttttcatct aaaaattttg ctgttttttc ttctaattgt ttcatgcttg
     7381 gtgtgactac atgagcaggc atttcttcca gcccgatttc aagtaataag tcttttgcca
     7441 tcttattcag cctccttcac gttttctgga tttgctgtgt ttttattcaa taaagggaat
     7501 cctaattttt cgcgttctgc cacaaagatt ttggctacag cacgagccat gttgcggata
     7561 cgggaaagat aacctgcacg ctctgttacg gatactgcac cgcgagcatc taataaattg
     7621 aatgtatggc tacatttcaa aatataatca tacgctggat gaacaagatt ttcatcgata
     7681 cagcgtttag cttctttttc aaaacgatca aaattgctta gtagcaggtc ttggtcgctt
     7741 acttcaaaag aatatttaga atgttcatat tctggttgtt tgaagatctc accatatttc
     7801 acgccatcag cccattccag atcataaaca ctttcaactt cttggatata agaagcaaga
     7861 cgttctagtc cataagtgat ctcagaagta actggtttac aaggtagtcc tccgacttgc
     7921 tggaagtacg tgaattgtgt gatctccatc ccatcaagcc atacttccca gcccacaccg
     7981 gcacaaccca tcgatgggtt ctcccagttg tcttcaacaa agcgaatatc gtgttctaat
     8041 ggatcaattc ctaaaagacg caagctttct aaataaagct cttggatatt ttcaggtgaa
     8101 ggtttcatta cgacttgaaa ttgatgatgc tgatacaaac ggtttgggtt ttctccataa
     8161 cgcccgtcag ctggtcggcg ggatggctct acataagcag cattccatgg ttccggtccg
     8221 attgcccgta agaatgtata tgggctcatc gtacctgctc ctttttctgt atcataagct
     8281 tgcatcagca tgcagccgtt tgatgaccaa aatttttgta atgttaaaat catatcttgg
     8341 acggtcaatt ttttactcat ctgttttgcc cctttcaatt tttaagcgtt gctgtcaata
     8401 agaatacagg aaagaaaacg acaaaaagtc cctatgtctg attacagaca tagggacgaa
     8461 taatttcgcg gttccaccct acttccgaat aaagattcgg cagcttcatt tatcgtactc
     8521 gcaagtgcct cttcaaaaat cgtttgggtc cggcttccac tctcccgaac tcgctaaacc
     8581 acgtgttttt tactgatctt ggtcaatgta cgtattcatt ttgcagtttt ctggtttgta
     8641 taatagatcc tctcagtgaa cgatctattt aatctacggt taataataga cggaagtccc
     8701 tgtatttgtc aaatagtttt gatttttttg ttgtatcttt catttattct tctttttcag
     8761 gtatctcttt ttcaggcata tttttctctg gcgtctttaa tacgtgtgcc caagacttca
     8821 tttgatcaat gaactttttg ctttttagat gaataccgac atattcatca tacaactggt
     8881 caatgaggtc acggattgcc tgcttcgttt cttctttcaa gtggattcca ctgatcttat
     8941 cataagaaat agccgaaaac attcgaacga aataaacagc gcgaggatct gcatgataac
     9001 ggtgttcgtc cattggccag tgtttctcgc atatcacacc gccatatttg gaagaatagt
     9061 caaattttcc ttttgtttcc ccgcatacga cacagtgcgt ccaaattggc gcgattccga
     9121 agcgttgcag tatttgcact tcaaagatat tcgtgatgat ttctgcatca tctccttggt
     9181 ccatccgctg cagagcttgt tgcgtaaatt ggaataaatg cgggtcatat tgatgatctt
     9241 cgattgctgc atctactaga tttagcaaat acgtaccata accgctcaaa aagatatctt
     9301 cttggatgtt ccgaaacgca tggacgtctt tgacactgct taaaaacgac aaaccttcac
     9361 ttcgaaaatt accgatatag actgcttcgg taaaggggag caaagccgga gctaatggct
     9421 gattttgccg atggattcct ttgatataaa acatcatttt gccagaagat tccgtaaaga
     9481 ttttgactaa tttatctttt tctttgtaat tacggctaga taaaatcaag cctttcgatt
     9541 ctacaagctg gctcatattc tccctcctaa aaaataaatc tggaaaagca gattctgccg
     9601 atccagatta tttatgtttc gatcttccgc tgtttttctc aacgtacgga tttttatcca
     9661 gtttatttct atacctaacc tataagaagt ccagaatatc agtactcgtc ttttcggtag
     9721 ccgaagtctt gcagatagac ttttttgtct cgccagtctt tttggacttt gacccaaagt
     9781 tctagaaata ctttgtctcc taaaagattt tcgatatcct ttcttgcttt cgttcctact
     9841 tgcttcagca tttttccgcc tttgccgata atgattcctt tttggctatc tctttcgacg
     9901 atgatcgtgg cctggatatg gattttatca ttctcgtttc gcttcatcga atcaacaacg
     9961 actgcgacag aatgaggcac ttcgtctcgt gttaaaaaca agactttctc acggatcaat
    10021 tcagaaacga tgaagtattc aggatgatca gtcacttgat catctggaaa atattgtggg
    10081 ccctctggca tttcactgac taatacatcc attaagcgtt cgacattgtt tccttctgta
    10141 gcagagatcg gaatcacttg tgcaaaaggc atctgcgtcg aataatcttc gatgattccc
    10201 agcaactcgt ctggatggat cttatcgatt ttattgataa tcagataaac aggagaatgc
    10261 acgtttttta gacgttcaat aataaaatcg tctccccggc cacgtttttg atcagcactg
    10321 atcataaata agactgcatc cacttctttc aacgcactgt aagctgcttc taccataaaa
    10381 tctcctagac gatgctttgg tttatggatt cctggtgtat cgataaaaat cagctgagct
    10441 tctggtgtag tatatactcc ttggatttta tttctagttg tctgtgcctt gtcactcata
    10501 atggcaattt tttgacccac gattcggttc aataatgtgg attttcctac attcgggcgg
    10561 ccgacgattg cgacaaaccc tgatttatgt tctggcatat acgttctcct ttttaattaa
    10621 acaacaaatg atatatttta gggacaaata aaatcaaccc aatgatgaca gcaaaacaag
    10681 cggtcactaa aactgctcca gctgccatat ctttgatttt tttcccaatc ggatgaaaat
    10741 gaaaatccgt aaacatgtcc actacatttt caaaaacagt attgatgatc tctactatcc
    10801 aaacgagaaa aacagctaaa agcagccata accactctat ccgatcaagc tggaaaatca
    10861 cgcccataaa taaagcaagg ataccgaacg caacatgttt cctcatattg cgttcttcct
    10921 caaaaacggt tttgactcct tggacagcaa attccacaga catcaggaaa tgtttgtttt
    10981 tctctacgtt tttatctttt gagtccatag gcatccaaga tctctttctg taaaccaaac
    11041 atttctttct catcttctgg tgtcatatgg tcatacccgt tgatatgcaa aaatccatgt
    11101 accgctaaaa agccaagttc ccgatcatat gaatggccgt aagcttctgc ttgttcagca
    11161 gcacgttcaa tagagatcat gatgtctcct agtacacgag gtaatggatt ctctccatct
    11221 tcaaaaatca ctgggatttc gtcttcgcct tcttcttcca gagcaaaact gatgacgtct
    11281 gtcggtgcat cttttccacg gtaatcacga ttgattactt gaattgccgc attgtccatg
    11341 aaagtaacag acatttctgt ttcctctggc agctttaaat aagatgctgc aaattgcagc
    11401 aagtcatcga tttctttcat tttctcttct gagacttggt ttgtttcatc cataaaggta
    11461 atatccatta tacatcgctt ctttctattc ttcttgtact tcctttatct tctcaacttc
    11521 tgttttcatt ttaggatatt tgatccgaga atggaaggtg ctacttaaac cgctgattaa
    11581 tgatttttcc acttttcgga tttctttcaa ggtcagtcca ctatcgtcta attgaccatc
    11641 ggaaatacgc tcttcgataa gattatggac aaaactttcg attttttcgc tcgtcggatg
    11701 atccattgcc cgtactgcag cctcacaact gtcagcaata ttgacgatcc ctgcttcacg
    11761 actttgcggt tttggtccag gataacggaa ttgctcttct gttacttccg gattgcgttc
    11821 tttcgctttg atgtagaaat aacgcattag cgtggttcca tgatgctgtt tgcaaatatc
    11881 gatcaccatt tgcggcatat tataatcttc caatattttg gctccatctg tcacatgtcc
    11941 aaagataata tgtttactgt cttctggcaa taagaaattg tgcgggtttt ccgctcctga
    12001 aggcaagttt tcaacgaaaa agctagcatg tttgatcttg ccgatatcat gataatagca
    12061 agctactcga gttagcaaag aacgtccgcc gatttctgct acagcgttag cacttagatt
    12121 agctaccatc atactatgat gataagtacc tggcgcttct tccagcaact gtttcaataa
    12181 aggatgattt ggattgctga gttcatttaa tacaattaca ctgtcatcat tgaccatcaa
    12241 ttcaatatat gggtgtagac cgatcgtcag caagaatgag aagagtgcac cagtgaaccc
    12301 gcagacgagc attaaccaag tcgttgcatc accaaagctc attccttgat agacgatcaa
    12361 aatgacatcc atcatcacag gaaaaataat gacccacatg gcagcagata atccttgttc
    12421 tgagatccgt ttgcgtctga caatcgttgc catgagccca gagaaaacat aagacatcaa
    12481 gataatcgtt agaaagttcg tgccaatcga atcataaaaa atgaaaagtg cggatactgc
    12541 ttggaaaata ccagccatga tccctgctct tcgattcaag aaaaagctta aaacaagtgg
    12601 tgcaaaagca gcgggataaa acagcgggat ataagtagct tgttccgttt ggaataattg
    12661 gaaaaatttc atcagcacta tgctgactga catagctgtc acatagaaaa gcacgtattt
    12721 tgttctttct cctgcatttt ggaattgaag tgaattataa atcagtacgc cgatttgaag
    12781 caaaacagct aaaaccatgg caattagcgg aaaaatcgaa gttgtttgat tagtcagtcc
    12841 tagtaattcc aattttttca tagctgaagc atcgatttga ttaccttcac gaacaatgat
    12901 ttctccttgg aagatcatca ctggttgtac agcttctcgt gcggattgtt tcaattcttc
    12961 tgtctttttc tcattcaata cgtcattcac tacgatacct tgatccacta agtagcgaat
    13021 catttgttgt tgtgctggtg tgacattcaa atattggatt tgctcttctg cttcttgttt
    13081 aaaagcttcc aattcacttt cacgaacttg tttgctcatc tgttcgtcaa tgagcttcag
    13141 actctcatcc ctcacagtat gaatctcagt ttcagtcatt tcaaatatcg tcgaataaaa
    13201 attgttcggt agtttttgat aaaacacaac attttctgca ttcacatttt caaaattttt
    13261 cttcagcgcg gcgatccgct cttctgtagt aggctttggg atattttcat cttcctttgc
    13321 cttatcaatc ttttcgtcat aagatttgtt gatggcatca ttcgtcttat cgatcaattc
    13381 aaatagttgc ttgatccgat tgttctggtc atctgcaaga tctttttggt aagtgtattc
    13441 tggagtgact gcttctgctg ccagttttct tttttgttcg gtttcttccg tgttctcgat
    13501 cgttttgttc gcacggatac tttcttctgc tacttggcct tctctgaaat cattggcctt
    13561 ttgtttgaca ctactaaatg taacgccgca gataatgata aaaaacaaga acagcaggaa
    13621 aggcaatgct gcctttccaa gtttttcttg tagtttctgc agtgttttat tcaacatgtg
    13681 cttatcctcc taagcaaagc ttattcttga tggagatctg ccttttcata agctttgatg
    13741 atttcggcaa ctacagggtg ccgaacaacg tcacttgctt caaaattaac aaaatcgatt
    13801 ttcttgattt gctttaatgt tcgttctgca tgaacaagac cacttgtcgt ccctcgaggc
    13861 aagtctatct gacttgtatc cccattgaca atcatttttg attgataacc gagacgggtc
    13921 aagaacattt tcatctgagc gactgtcgtg ttttgtgctt cgtccaagat aacgaatgca
    13981 tcatccaatg ttcgcccccg catgtaagct aaaggtgcga tttcgattac gcctcgttcc
    14041 atcaatcggt tcgtatgatc caatccaaaa atttggtata aagcatcata aacaggccgc
    14101 agataaggat ccactttttc ttttagatcc ccgggaagaa agcctagatt ttcacctgct
    14161 tccacagcag gccgcgtcaa aatgattttt tgcacttcac cttttttcaa ggcagcgatt
    14221 gctaatacta ctgcaagaaa agtttttcct gtcccggctg gtcctacgcc aaatacgata
    14281 tcatgtttgg ctaccgattc gacatatttc ttttggccgc tgttttttac ccgtataggt
    14341 tttccattac ggtctttgac gatctcttcc tcatacatct caacaaaata atctaatgta
    14401 cctttttgcc ccattttcaa tgccgtcaca acatcgggcg tacttacgtg gattcctcgt
    14461 ttgatcaatt cctgtaatgt ccgaatgact gaggcagcca aggaaatctg ggattgctca
    14521 ccaatgatct gaatgatttc tccacgtgta tggatggttg tttccgttgt ttcctcaatc
    14581 agttttatat gcttgtcgtg tgcacctaaa agcatgctga catcatcagc agccgtcaat
    14641 ctaatctcta aagaactaga ctcttccgtc aaaaatagac atcccctttt tttcgattaa
    14701 ttctttccta ttttaccata ctcagaagaa actatatagc aaataagaaa aggttctaaa
    14761 agagttgctt aaccataagt accaagaaac gttaaaaaac tagccgcttc cattctctaa
    14821 aggtctcgtt tgaataaaaa aagctgatca tgtaagctat gcataagata gaggacaagt
    14881 attccgtcta acttagcaag tattctctgg gtatttttct tgtttcaaaa aaagaaaaca
    14941 aaatatttga aatattcttg catttcaaaa aaaacaggtg tattataacg tcaacaaata
    15001 aatatacagc aaagcgtcga ggaaaacagt aggctattca gtgatttatt cccagtgagt
    15061 catgtaagtg agaaatgaca ataaatcgaa tagacgaaca tcatttccga gtcaatgtcg
    15121 aaaacgtaga tcattgtggc tgcctccatt atcaggctag cggataactt tatcgaaaag
    15181 cgtccgtgaa tgaagaagct tttttaagcg aaaaaaggtg gtaccgttct agaatctaga
    15241 gcccttttcc caagtggaaa agggcttttt ttatatttta aggtggtggt tttcatggaa
    15301 tctgatatta gtggtatttc tagtttctgc aacgtttatt ttgttactca aaatgataat
    15361 tattggagga aaaatttatg gaagaaacaa aaacttattc atggaagcaa ttattgatgg
    15421 tcagttcact tatcttcggg atgttcttcg ggtcaggtaa tctgatcttt cctgtacact
    15481 taggtcaaat ggctggatca aactggatca gcgctggtgt aggatttgcc atctcaggcg
    15541 ctcttttccc attattagct atcttagctg tagtagtgac aaaaagtgat ggtctgtatg
    15601 acttagctaa acctgtcgga aaatggtatg ctgccttgtt cttgatcctt gttcatttaa
    15661 cgatcggacc attctttggt acaccgcgga cagcagcaac tgcttttgaa atggctgcta
    15721 aacccttttt gcctgaaaaa tatgattcat taggtatgtt tttattctct gctcttttct
    15781 ttttacttgc ttatttcctt actgtacatc caacacgttt ggtcaaatat gtcggaaagt
    15841 atttaaactc cttgtttctt gcattattag ctatcatttt tgtcgtttct ttattccaac
    15901 ccatggggaa tctaaaccaa tctgctgcag attcttatca gacgaacgct ttgacaaatg
    15961 gcgttttaga aggatacaac acggttgatg cagtagcttt acttgcttta agtgtcactt
    16021 ttgtccatgc agttcgtgat cttggtttta aagataaaaa agtcacagaa ttaacagcaa
    16081 aagctggtag cctgagcgtt gcgttagaag tcatgattta ttttggtctg gtccttctcg
    16141 gtgcgatgag tttgaataag atgtctttat cagaaaatgg cggaactgca ctttctcaga
    16201 tcactaattt ttatttaggt aacttcggtg ctagcttctt aggggtaatg gtcacattag
    16261 gtgtcttcac tactgctatg ggactcgttg tttcttttgc acaagatttc cataaactgt
    16321 tccctaaagt cagttatatg acatggctgc ggttaacgac ttttgtctct tttgtcgttg
    16381 cgaatgccgg attggataat atcatccaat ggtctttacc agtattgatg cttttgtatc
    16441 cattgtcact tgcgttaatc ttactttcct taacagcaaa attcttccaa aagagtccat
    16501 ttgtctatca agtaacgatg ttcttttctg ccgttccagc cgtattggat atgttggcaa
    16561 attcgcctgc attagtgagt cagcaaactg tcgtagcaag cttactagga ttttatcatc
    16621 attatgttcc ttttgctgca ttaggtcttg gctgggtcgt cccaacattg cttggttacg
    16681 ctggcagctt gctcttttat tatgtatatc gcgtatcagg atataaacaa gaagcaagtg
    16741 agttaccaga agaataacca aatcaaacac aatttaacat aagagatatt aaacaggaaa
    16801 aaaacagcta gaagaaagaa taagattctt tcttctagct gttttttagt tcttttttta
    16861 cgaacacaaa aaactgcgga taatcactgt ccgcagttta aatgataaaa ttaagattgt
    16921 aatagttctt tcacaatcgc attcacttgg tttccatctg cttttccttt gactttaggc
    16981 ataattgcac ccataacttt gccaaattct ttcattgatg aagcaccagt ttgagtgatt
    17041 gcttcttgaa caagttgtcg aatttcttcg tcggaaagtt gttccggcat atatttttcg
    17101 actattacaa tttcactttt tactttttca gctaaatcgt cacgtcctgc ttcttcaaac
    17161 tcatgcaaag aatcacgtcg ctgtttcatt tcacgagaca acaccgttag ttcttcttct
    17221 tctgtcaagt cgtgaccttc tttaatctgc tcgttttgta tagatgactt gatcattcgg
    17281 atgacttgta aagattcctt atctttcgct ttcatcgctg ctttcatgtc atcgtttaat
    17341 ctacttagta gtgacacact accaactcca attcatcaca gaactagaat tttttgcgtt
    17401 ttctagctgc ttcagatttt ttcttgcgtc ttacacttgg tttttcgtag aattcacgtt
    17461 tacgagattc ttgtaaagta cccgcttttg acacggaacg tttgaagcga cgaagagcat
    17521 cgtcaagtga ttcgtttttg cgaacgactg tttttgacat gttaattccc tccctccgag
    17581 cttaaaaagt aaccgtaatt gttattgaaa tatatctcaa aaaacaagac tgtcctgtaa
    17641 atattatatc taatgctgtt ttgaccgtca aggaaatctt gctttttttt aaaattatca
    17701 aatgttattt tatttaaatc cttattgaca attttcacat cttccgaaaa tctcgaatct
    17761 atgcccttcg atcgtgcatc cacctagctg ttcttcgaaa taattcatcg gacacatatg
    17821 gatttctttc gttgttccgc acactgtaca gataaaatga tggtgatggc cgattttccc
    17881 ctgacagcaa tgaaaacgaa acttcatttc tccatttaaa tcggtttctt ccagtaaatc
    17941 gatctctgca aaatcatgga gattccggta aatcgtatca taacttaccc ctttgaattc
    18001 ctgattcata tagtcaaaaa cttctttagc agatacgtag cggttacgct tcactaaata
    18061 agttaacaat gcttctcttt ttttagtata tttaaaaccg ttatttttca atgtttctaa
    18121 tgattgttcg atcaatgtct gttttttcat gacttatccc tccaaatcaa aatgattacg
    18181 aagtacgcat tctagagtat ggtataataa acataaaaaa aatgcaagtg aggttttatc
    18241 catgacttcc gattctacag aaatcgttac tttttttgtc atctccgact ctgctggaga
    18301 aactgcgaca aaactagcgc aagctactat ggctcagtat cctaccgttg agttcaacct
    18361 attccgcaga acgttcgtga cagatgaaaa aacattaagg caggcattgc aagatgcatt
    18421 agaagaaaaa gcacttgtcc tgcatacatt aatcaatcaa gaactgatcg agataacacg
    18481 ttctttttgt gaagaacatc aattatttag ttttgatgtg atgacgccac cgatatcaga
    18541 aattgaacgt ttaactggta tcaagccaat tcggcaacct ggtgccctcc atttattgaa
    18601 tgagaactat ttcaaacgga tcaaagcaat ggaatttgct gtaaaatacg atgatgggaa
    18661 agatcctcgt ggttttttag aagcagatgt cgttttgcta ggcgtatccc gaacttccaa
    18721 aacgcctttg agcttgtttt tagctaacaa aaatttaaaa gttgccaatt tacctttgat
    18781 cccacaagca catattccca aacagctttg ggagatcgat cctaaaaaaa tcgtgggact
    18841 aacaaataac ccagatattt taaataatat ccgtaaagaa cggatgcgct cttacggatt
    18901 aaaccctgat actgcttatt ccgatatcga aaaaattaga gctgaactcg attttgcgaa
    18961 tgatctttat gaaaaactag gatgtgtagt gatcaatgta gcatctttat ctattgaaga
    19021 aacggcttcg cttattttga atgctttaga cctagaagat catagttatt atggaactga
    19081 aacaacagaa aaaagcgatt gatcattttt gtctttttct taaaaaacac tggagtgtga
    19141 caacgaaatt tcttcgttgt cacactccag tgtttatcta tttgcaccta tcgttattga
    19201 tgttccacat gtttgagtgt ctggttcaaa atatccagta catgagaatc atcaagacta
    19261 tacaaaatcg cttttccctc gcgatgagac ttgacgacat gattttctct taataaacgt
    19321 agttgatgcg atacagcaga ctgctccatt tccactgctt gggtgattgc tgtcacattt
    19381 tgttccccgt ctttcagata tagcagaatt ttaagacgag tgggatcact caacattttg
    19441 aataattgac tgactttctc tacttttgat ttttgcgtgt ctggcaatcc attcacctcc
    19501 aacttgaaaa aagactgact tctaaagttt accacttttt ccatcagtct caagtttttt
    19561 attcttctgt catctttttg ataatcgctt cataagaggc tactttttcg gctgcgtctt
    19621 ctgtagaact tccttttaca ccaatataaa acttgatttt cggctcagtt cctgaagggc
    19681 ggatagcaat ccagccattt tcttccaatg tatatttcaa tacgtcggat ggcggtgttg
    19741 tgagtttttc tacgtttcct tcacggtctg ttctcgtcaa ttctttaaag tcttctgttt
    19801 gaataacttt tgctccggca aattctgtcg gtgcttcttt acggaatttt tccatcaaag
    19861 cagagatttt cgcagaacct tcttgtccgc tcattgtcac agaaatcgtt ttttctgcga
    19921 aatagccata ggtttcaaaa atcgtttgca agccgtcata caacgttttg ccttgttttt
    19981 tatagtaagc tgctacttct gctaataaaa gcaatgcttg gatcgcgtct ttatcgcgaa
    20041 caaaaggttt aactaaataa ccataacttt cttcgaatcc aaacataaaa gtatgtgaat
    20101 ggttttcttc gaattgttgg attttttcag caatgaattt aaaccctgtc aacacattga
    20161 acatggtagc accgtagtgt tccgcaattg ctgtaggcaa ttcgctggaa acgattgatt
    20221 tcaataccgc tgcgttttct ggtaaagtgc ctcttgattg atgtgcttcc aaaatatatt
    20281 gagcaaacaa tgctccgatt tggttcccag tcaatacttc ataagaacca tctggcaaac
    20341 gaaccgctgc gcctaaacgg tctgcatcag gatctgttgc aatcagcaag tcagctcctt
    20401 ctttttctcc aagtcgtata gcatattcaa aagctgaatg ttcttctgga tttggcgatt
    20461 tgaccgtagt gaaatctgga tctgccacag cttgttcggg aactagtacg aattgttcga
    20521 atccagcttg tttcaacgca cgttcgccca acattttccc tgttccatga agtggcgtgt
    20581 acacaagttt caaatctttc cccattgttt caaccaattc acgatcaatc gtcactgctt
    20641 tgatttcttc tagataagca gcgtccactt cttctccgat gatcgtgatc aacccgctat
    20701 gtttagcttc ttcgtcagat aatacttcga cttttagcgg attgctgact tcacggacat
    20761 atttcgtcaa tgcatctgcg tctgcaggag gcatttgtcc gccatcttct ccgtagactt
    20821 tatatccatt gtaagccgct gggttgtgag atgcggttac catgattccc gcaaacgtct
    20881 tcaaatgacg aactgcaaaa gaaagctctg gtgttggacg cagactttcg aatacataag
    20941 acgggatatt gtgatgtgcc agtgttttgg ctgcttccat tgcaaattct ggtgactgat
    21001 ggcgtgagtc ataagcgata gcgacacctc ggcgttttgt ttcttcacct tgtgtatcca
    21061 tgaaacgtgc tagtccttct gttgcttgtc ttaccgtata gatgttcatt cgattgatcc
    21121 caggtcctaa gattccccgc atgcctgcag taccgaattc caacggtgca taaaaagcat
    21181 cttcaagttt ttcaggatct tcacccagat catttaattg tttttttaag ttttcttcta
    21241 aattttcttc atgtacccat tgttcataca cttgttccca agacatgaaa ataacacctc
    21301 tttctgaaat atggttctta ataagtatag cattcactta ttagatatga aagtatgagg
    21361 accaaaaaac gcttaaatca agaaaggatt cttttatcaa gcatgattgc aagtattcac
    21421 ataaatcgga ttcagtggat ttcacgcgaa aaaaaagagt aaaagaacaa ttgaaaccgt
    21481 tctttcactc tttttaaact tatgctgttg tttcttctaa agactccaag tatttataca
    21541 cttgttgtcc agcaataccg ccgtcaccaa cagctgtagt gatttgtcgc aaatcttttt
    21601 cacgaacatc tccgatggca aaaacgcctg gcatattcgt tttcatttcg ttgtctgttg
    21661 gaatccagcc agcttcgttc gtcaaacctg cttctctaaa aggtgcagtt aatggatcca
    21721 gtccaacata gatgaatact ccgtctgctg ctaaatcaaa ttcattgccg gttttgacat
    21781 tacgtgcttt cacacctgtc actaccattt cgtttccaac gatttcgtca acgactgtat
    21841 cccacataaa ggaaattttt tcattcgcaa atgcacggtc ttggatgatc ttttgtgcac
    21901 gtaattcatc tctacggtgg acgatgacta cttcagaagc aaattgtgtc aaatagatgg
    21961 cttcttctac tgctgagtct cctcctccga tcactactag acgtttgtta cggaaaaacg
    22021 caccatcaca aactgcacag taagaaacgc cacgtccagc aaattcttcc tcgccaggta
    22081 cgcctaattt acggtggata cagcctgttg cgataatgac tgtttttcct tcgtaagttt
    22141 tatcttcaca aataacttct ttgtggctgc cgtgatcctt gatatcttgc acgattccat
    22201 aagcattttc tgtcccaaat cttgaaacac cttcatacat cttataagct agatcaggac
    22261 ccatgattga atcgaaacca ggatagtttt cgatttctgc cgtattattc atttggccgc
    22321 caggggcgcc ttgttcgatc atcaatacgg ataagtttga acgtgaagca tataaagcag
    22381 cagtcatccc agcaggacct gcgccaataa cgattacatc atacatatgt agattctcct
    22441 ttacttaatc aagtaagcat actttacagc caatgcgaaa aaaagtctat tgttctgctt
    22501 gcgtttctcg aatagcttta ttatactcga aaaatccgct gaaaaacaat agacttttat
    22561 aaatcattga ttaatcattc gaaagatttt ctccatttga atcaattact tttttatacc
    22621 agttgaatga tttcttcttc gaacgggcta atgtgccatt tccttcgttg tcacgatcga
    22681 cataaatgaa tccgtacctt ttcttcatct cacctgtacc agctgatact aaatcaatac
    22741 atccccaagt cgtatagcct aatagatcga ctccatctaa ttctactgca tctttcattg
    22801 cctggatatg tgctgccaga tagtcgattc gatagtcatc ttccacataa ccattctcat
    22861 cgggcgtatc gactgcacct aagccgtttt ctacaataaa tagtggtttt tgataacgat
    22921 cataaatatc attcatcgtt attctcaagc ctaatggatc aatttgccaa ccccattcac
    22981 ttgcttccag atatggattt ttgacagaag caaagatatt tcctgcagtc tgttccaaga
    23041 gttcgttgtc agtagtcgaa actcgagaag aatagtaaga aaaggagata aaatcaacag
    23101 tatgttcttt cagcaatgcg gcatccccag gttccatttc gatctgaatg ccatttcgtt
    23161 ccatttcttt caatgcataa gcgggatatt ctcctcgtga ctggacatca atgaagaaat
    23221 aattttctcg atctgatttt ctagaagccc aaacatcttc aggattgcaa gtgtatggat
    23281 aatatgctcc tgctgccagc atacagccta ctttgttctc cggatctact tcatgagcaa
    23341 tcttcgttgc gatggcactt gcaaccaact catgatgtgc cgcttgatat ttgatttgtt
    23401 cttcattttc tccttcttca aaatacagcc ctgctcccat gaatggtgca tgaaggatca
    23461 tattgatctc attgaatgtc agccaatatt tgacaagtcc tttatagcga ttgaagatga
    23521 ctcgacacag attttcataa aaaccaacga gttctctgga acgccacgca ccatattctt
    23581 tgatcaaatg cattggacaa tcaaaatgcg taatcgtcac aagtggctca attccgtgct
    23641 tttggcattc tttaaaggca tcctcataaa atttcaagcc agcttcattc ggctctttct
    23701 catcccccat tggaaaaata cgactccagg cgatggacat tcgataagtc ttgaacccca
    23761 tttctgcaaa caaagcaata tcttctttat aacgatgata catatcgatt gcaatttttg
    23821 ctggataaaa atgatcctca tcaaattcaa acattttttt ctttccggtg ataacaggaa
    23881 aacggtcttt tccgattggc acaacatcta cgtttgctag tccgcgtcca ccttcatcat
    23941 aaccgccctc acattgattg gctgctgtag caccgcccca taaaaaatct tttctgaatc
    24001 ccattatact tcctccatta tatgtgtatt acctgataat tctcttgcca gttcatcttg
    24061 aacttcgatt ccttcttctt ttaatcggct gattgtctcc ttgaattgcg gtaaatactg
    24121 ctggtgtgca ataaacaact catccatgat ccgttttgct gtctttcctg atcgtataag
    24181 tggattgatc gtaaacgcct gtaaagctgt cccataatca ccagtcacag ctgcttcgat
    24241 cgtcagcaat tccatattct tcattacttg caaccagcct ctttcagcag caggaagaga
    24301 accaaacgct acatttctcg ctccttgtgc gccaacatag gcagtgactt ctactacaca
    24361 atctgccggc aggtcaggaa cagctccatc attctttgta gataccacga tctgtgtttc
    24421 tttatttcca tagattgaag caatcgtttc acaggcagcg tctgaataat aagcgccacc
    24481 tcgtttttgc aattgttcag gtttatggtc taattctgga tctttgtata attcaaatag
    24541 ctctgcttct gtttctttca cttgctgcgc tcttgtacca atcgtttgat attcttctaa
    24601 agcatgttcc agcatttctt cttcacgata gtaatagcgg tggtagccac aagggatcat
    24661 gttcatttgt tttaattgtt ctttgaaaaa cgggatatca aagatatttt tcggtaatcc
    24721 attctcttca tcgtataaac gatcaatcaa ttctgccgtg cggtcatttc ctttattatc
    24781 tgctactttg tgccaatgga aatgatttag accggcaaac ttgtagatca gctcttcttt
    24841 tttcttgcca agcattgctg gttctgtcat catcgccatc actggcacat tgcataatcc
    24901 aatcacttta tcccatttgc catatctgat aactgcttct gtcaccatcc cacttgggtt
    24961 cgcgaaattg atcagccaag catttggaca taaacgcttc atgtcctcaa cgattgccaa
    25021 aacgactgga acggtacgaa aagctttgaa tatcccgcca gcaccatttg tttcttgccc
    25081 aatcatccca taataactag ggatccgctc gtctttgatt cgtgcatcta attgaccgac
    25141 acgaaactga gtcgtaacaa aatccgcatc tttcaatgct tcttcccgat ccagagttgc
    25201 atagatcttg acttcatacg gagaagcatc ccacatacgc tgagacatct gactaacgat
    25261 ctcaagcttt tcttttcctt cttctacatc tacgagccag atttctcgta tcggtaattc
    25321 ctcatatctt ttgataaatc cttccatcaa ctcgggggtg taactgctgc ctccaccaat
    25381 cgtagcgatt ttgattcctt ttgccatgtt ctcactcctt tactttttta cactttcatt
    25441 atcttttttg taaacaaaac aaacaat
//
LOCUS       DACDSY010000021        25457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDSY010000021 DACDSY010000000
VERSION     DACDSY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.021"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            79..2139
                     /locus_tag="IWK44_000775"
     CDS             79..2139
                     /locus_tag="IWK44_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549327.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2222..2791
                     /locus_tag="IWK44_000776"
     CDS             2222..2791
                     /locus_tag="IWK44_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9549328.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2966..3772)
                     /locus_tag="IWK44_000777"
     CDS             complement(2966..3772)
                     /locus_tag="IWK44_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549329.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3910..5424
                     /locus_tag="IWK44_000778"
     CDS             3910..5424
                     /locus_tag="IWK44_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP9549330.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5827..6810)
                     /locus_tag="IWK44_000779"
     CDS             complement(5827..6810)
                     /locus_tag="IWK44_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP9549331.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6815..7462)
                     /locus_tag="IWK44_000780"
     CDS             complement(6815..7462)
                     /locus_tag="IWK44_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9549332.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7547..8179)
                     /locus_tag="IWK44_000781"
     CDS             complement(7547..8179)
                     /locus_tag="IWK44_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9549333.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8195..8746)
                     /gene="coaC"
                     /locus_tag="IWK44_000782"
     CDS             complement(8195..8746)
                     /gene="coaC"
                     /locus_tag="IWK44_000782"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP9549334.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8743..9480)
                     /locus_tag="IWK44_000783"
     CDS             complement(8743..9480)
                     /locus_tag="IWK44_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP9549335.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9477..10466)
                     /locus_tag="IWK44_000784"
     CDS             complement(9477..10466)
                     /locus_tag="IWK44_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9549336.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10724..11266
                     /locus_tag="IWK44_000785"
     CDS             10724..11266
                     /locus_tag="IWK44_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9549337.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11283..12368
                     /locus_tag="IWK44_000786"
     CDS             11283..12368
                     /locus_tag="IWK44_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9549338.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12380..13186
                     /locus_tag="IWK44_000787"
     CDS             12380..13186
                     /locus_tag="IWK44_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9549339.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13187..14014
                     /locus_tag="IWK44_000788"
     CDS             13187..14014
                     /locus_tag="IWK44_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9549340.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14011..15084
                     /locus_tag="IWK44_000789"
     CDS             14011..15084
                     /locus_tag="IWK44_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9549341.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15512..18208
                     /locus_tag="IWK44_000790"
     CDS             15512..18208
                     /locus_tag="IWK44_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9549342.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18285..18773
                     /locus_tag="IWK44_000791"
     CDS             18285..18773
                     /locus_tag="IWK44_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9549343.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18785..19279
                     /locus_tag="IWK44_000792"
     CDS             18785..19279
                     /locus_tag="IWK44_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9549344.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19300..20109
                     /locus_tag="IWK44_000793"
     CDS             19300..20109
                     /locus_tag="IWK44_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9549345.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20106..20927
                     /locus_tag="IWK44_000794"
     CDS             20106..20927
                     /locus_tag="IWK44_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9549346.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            20978..23077
                     /locus_tag="IWK44_000795"
     CDS             20978..23077
                     /locus_tag="IWK44_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9549347.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23074..24192
                     /locus_tag="IWK44_000796"
     CDS             23074..24192
                     /locus_tag="IWK44_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9549348.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24231..25109
                     /locus_tag="IWK44_000797"
     CDS             24231..25109
                     /locus_tag="IWK44_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9549349.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25300..25452
                     /locus_tag="IWK44_000798"
     CDS             25300..25452
                     /locus_tag="IWK44_000798"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549350.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8034 a         4434 c         5403 g         7586 t
ORIGIN      
        1 attttattta tataatgaaa tcatcttatg aaaattacta cttcatgttt agacaagagt
       61 agggaggact aagaataaat gtgttcaaaa agacgttcaa aacaactaaa taaacacgct
      121 tgtcgtttag ataaggaacg gtcgtgttct gaaaaacact acgttaagtt tatgatgcgc
      181 acgatgctga tcagtttatc tgtaggtgtc tatatgcatg tacccgttca ggcaacgaca
      241 caaaccatca aggtcgtacg tacctctgtt tctgagacca ctgattcccc tatcactatt
      301 actcatactt tcactgatgc aaatggaaaa cagctacttc ctgcattgac ttcctataat
      361 aaagtcagtt atcctccaac gataaaccac tactggttaa ttcctaacaa aacattatgg
      421 acatcggaaa ccggaagcag tgtgccgctt tctatctttt ccgatatgac tgatcctaca
      481 aaaggtgatc agttaaaaca gatcactgat attttgaatc atgctttgac agatataaag
      541 gacggtggaa gcgcacaagt ttcttatgta tatgaaaaag attattcaca gtttgaaaga
      601 aagaaaatcg atgttctggc agggacaaaa gtttcgatat ctgatctggt agaaaggctg
      661 aagaacgcag atggaacaca aggagatatg tccaaagtaa tttcaaacaa tgggaatatc
      721 gtggatacga ctaatacagg aacatctact atttccttgt cctattttga tccaattgct
      781 ttaaaaacaa tgactgcatc tgctgtgatc catgttcgtg ctgatcaaac agctatcaaa
      841 ggaaaagatc ttcacttaag aacaggagag agttacgccc ctaaagatct tgttgaatat
      901 gtaacaaaaa gcgatggaac ttcaggaaat aaagaagaaa tcaaaatgaa cgggacagta
      961 gatactacaa ctccgggaga atatcccgtt gtattaactt atatagactc gaaaacatca
     1021 aatgtggcag aaacaacagc tgtagtttat gtagaagcat ctgctgcttc atcgactgtc
     1081 atcactcatt ttatagatag tcaaacaaat aaagaacttc aagcagaaac acatatgagt
     1141 agtgtaaatc gtattcctga tttgcgccta cctgctgaat tttctgatcg agtgccaaat
     1201 atagatttat cgacttatga acaaaatggg aaagtcactt ctatttctga ttggatgcaa
     1261 gcaaacggat tagacagaaa aagtcactgg tcggagatcc ttgaagtact tcactcagaa
     1321 ctgatgaata ataggaagca atcaggaggg gaaattacct ttacttatgt ttatatacca
     1381 gacaatcgtt cattagaagg tcatgatctt attgttcatc caaacgaaaa aataaacaaa
     1441 gaagagttga tccaatcagc aacaaattcc ttaggtaaac ccgtaccgtt aaaggatgta
     1501 tcggttacaa tcaatgaagt accattggag gatggttatt tgaccaaaca aacaggtgat
     1561 tttacgatta tgtatagtta tcatgacccg tattcactcc aagaactcca tgctcagtca
     1621 ctattacatg ttttaccatt aaacaaacag atagatcaaa aagaacaaaa aagacgagaa
     1681 gaaagcttgt cagaaaaaaa acaacaaatg gctcatgcag tttctccaac agggaaagta
     1741 aaacaaacaa gaagcctaga gttagcaaga atatctagaa aaatctcaga atctaataaa
     1801 acgataaggc aagaagctca tttggcccca caagtttctg gattggaaaa tgaaatttta
     1861 gagcctaaaa taaaacaaca atcagaaaaa gaatttgttg ctaaacagtc ggaagatcag
     1921 aaggaatcag atgaccagaa ggaaacgaag aaaaaacgct tgtcaactat tgattccacc
     1981 gatgagcatc tgcagccgca agtaccactt cctagtccgg gaacacccgc acctggtgga
     2041 ggtattcctt cttctgcttt aggagatttc atgcgaggta tggcaggaac atgggtatac
     2101 gctaatcgaa atgaatttga cgcagatgac tggttctgat ttatatctct atctgttata
     2161 gtcaaataag tagctgagtc atatgttatt tggcataatg gagacgagga gggatagaaa
     2221 aatgttagat aaaacgttac cttacgcaga aatctggatg acaagaccac ttcaacaaaa
     2281 tgttccaatc acgcctttag cggaaggttt tgaatttcga ttttatcaaa aagaagatga
     2341 atctgcttgg gcggagattg agacttccgt tggggaattt caaacgacag atgaagcact
     2401 tgagtatttt caacaaaact ttgctcctta tcctgaagaa cttgaaagaa ggatgttttt
     2461 tgtagagaca aaaaaaggag aaaaagtagc aacctgtacc gcttggcgaa aaaaatcaag
     2521 agagcaagtg tatccggttt ttcattggtt agcagtgaaa ccagattacc aaggaaaagg
     2581 gctcgcaaaa gcattgactg ccagagtctt gcaagaattt cctaccctcc atacggacgg
     2641 accaatctat cttcacacac agacatggag ccatcaggcg attgctcttt ataaaaaaat
     2701 gggttttact tttattgctg aaaatctaga cggcacacct aatccagatt atgaaaaagt
     2761 tatgcaggtt ttaagtaaat tagataaata acgaaatggt gaaataagtc ctcagtattg
     2821 ggataaaaaa cgaaatagtc attcctcata tcttactttt ttgagttaat actatcggat
     2881 aactaatggc atgccataca ttcaaaaaag aaggggcagt gacaaaagta ttgtcactgc
     2941 cccttctctt gttttttagc atgttttatc gtttgatttt tttaggaata aaattcttga
     3001 tgaacgtaaa tttttggtaa tcgtttcgac tatagaggta accaaataca ctgaaaacaa
     3061 ctgctccaat aaaattaaca aataaatcct tcatagtatc catgattccc acgtctaagt
     3121 aaccattttg gatgattgtt ttatctgttt taccatctgc attttgactt tcaatgattg
     3181 tccgagtgat atctggaaca tgatagacag tgtttccgtc tttacttaac tccacactat
     3241 tgatttgctg gatgatcgta tctttttgca tatcgaattg cagtaattta tctgctgaaa
     3301 attccacaaa ctcccacata acaccaactg tcatcgaaaa acagaaggaa acaactgcta
     3361 gtaataaagg agtcaagttc atggtttttg tatttttatt gagcaaataa acaagggaaa
     3421 agccgacacc cccagctaaa aatccgctga atgcatgtag aaaagtatcc cacattgaaa
     3481 aatagccata aaaatcactt aactctccta aaactgttga actaaaaata aaagataaca
     3541 aaatgatttc tagcatattt ggaatctgta tctttaatat cttttccaga aaataaggga
     3601 tttcaaataa aattaatgtc agtaataaaa gcattgtatt tccccagtgt tggttccaga
     3661 cctgatccac tagcataata acgacgatga tacgtaataa aatatagatt ccgtttgtga
     3721 ttctacctgc agtttttgta ctgttcgtaa aaaaggtttt caaatgattc ataaaataac
     3781 tccttcatgt atatccttaa ttatactaag aagcgtgtgt ttaccaaagc tatatgactt
     3841 aaagaaaaac agagttgata ttgtgaaaaa aatcacttaa tatagtataa ggagaaaagg
     3901 gggatacaaa tgaaaaaatt agttgctggt ttattaacag ccgttgcact tcttgctatg
     3961 agcgcatgcg gcgcagatca gaaaaataca gcgcagacaa gttctagtac gaaggaatct
     4021 gccgaagtat catcaggcgc ttctgaacaa gtatatacag atccaaaaga aatgaaagaa
     4081 tcttatgatg tcgtgattat tggagctggt ggtgcaggga tggcagcagc tatcgaagca
     4141 aaagatgctg ggttgaatcc agtaattttg gaaaagatgc ctgttgccgg aggaaacaca
     4201 tctaaatcat caagcggtat gaatgcttca gaaacaaaat ttcaaaaaga agaaggaatc
     4261 aaagactcga atgatgcctt ctatgaagaa acgctaaaag gtggtggagg aacgaatgat
     4321 aaagaaatgc ttcgatactt tgtcgatcac tctgctgatg cggtcgattg gctagatcaa
     4381 aacggtatca aactggacaa cttaacgatc actggaggga tgagtgaaaa gaggacacat
     4441 cgtccgtctg atggctcggc tattggtgga tacttagtgg aggggttatt gaataacgtc
     4501 catgagcggg aaatccctat ttttgtcaac gcagatgtga cagacattaa gaaaaatggg
     4561 gaaacggtca atgaagtgac tgtacatgtt caaggagaag aaccaaaaga ggtcaccgga
     4621 aaagcaatcg tagttaccac aggcggtttt ggtgccagca aagaatttat tgaaaaatat
     4681 cgtcccgatt tagctgatta tattacgaca aatcaagaag gatcaactgg agatggtatc
     4741 accatgattg aaaaattagg tggacaaacc gttgatatgg ataaaataca aattcatcca
     4801 actgtgcagc aggatgaggg ggtcttgatt ggtgaggttg ttcgcggaga aggagcaatc
     4861 ttggtcgatc aagaggggaa aagatttgtc aatgaaatgg atacaaggga taaagtatct
     4921 gcagcaatta cagcattgcc tgaaaaatca gcttatttga tttttgacca aggcgtgcgt
     4981 gatcgtgcca cagctattga attttatgac caaaaaggat atgtcacaac tggaaagaca
     5041 atggaagaat tagcagaaaa aatcgattta ccaaaagaaa cactagcaaa aacagttgat
     5101 ggatggaata cggatgttgc ggagaaaaaa gatgctcaat tcaaccgtgc cacagccatg
     5161 gaacatgact tatcgtctcc gaattattat gcgatcaaaa tcgcaccagg aatccattac
     5221 acgatgggcg gtgtgaaaat caatacgaac actgaagtat tagataaaga aaaccagcca
     5281 attagggggt tatatgctgc tggtgaagtc acaggtggct tacatggaaa caaccggatc
     5341 ggtggaaatt cagttgcgga gatcattatt tttgggcgac aagcgggcat acaagcagct
     5401 cgatttgctt cggaaaatga atgaaataga acgtaaataa aaagaggttg tgaaatcagc
     5461 gttttgagct aataccgagg gtcagctgat tgaacactgt ttagtgaaga ggttgtgaaa
     5521 aagctgccct gcatcaagta ataagaacag ccaagtaaaa acagcttctt atgtttttaa
     5581 cttggttgga cttattaccg cagatgcaag cttttgaaca ccgtttattc ggaaaaagag
     5641 cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcggaa
     5701 gcagaagaaa ttccttcgga aataagctga aattcacaaa aatttgaaaa gcaattttcg
     5761 tgaatttctt cttatttctt ggagttaaac gcttctgtcc cagcctcttt tttgatcagc
     5821 atgattttat tcagttatct tcaaaatgat ttttccgatg tttttgtttg cttccatata
     5881 ttcatgcgct tgtttgactg cgctgaattg gaatgtacgg tcaatgattg gccgaatctt
     5941 gccattttct agaaaaggcc cagctttttg cataaaagtt tgtgcgagct ctgctttata
     6001 ctgatcactt ctaggtgtca ataatgtacc aagcagttgg acacgtttcc caatcaatac
     6061 accaaggtct atctcaggca cgatggtccc acctagcata ccaatcaaga cccaacgtcc
     6121 atcaacggct atagaagcta aattcttttc ccagtaagaa gcgccgatga aatccaaaat
     6181 aacatcgact ccccgacctt cagtgatttt tcgtacctct tcttgaaagt cttgtttttt
     6241 atagttgatc aattcatctg caccaagttc tttacaaaag gcgagttttt cttctgatcc
     6301 tgcagtaacg atgattttcg cgtgagaaag ctgtctagct aattggatcg ctgctgttcc
     6361 cacaccactt gctcctgcat ggatcaagac agtttcgcct tcttgtaatt ttccaagcca
     6421 gtaaagtgtt tgataagcag tcaaaaaaac ttctgagatt ccagcagcag aaacatagtc
     6481 tagcgtatca gggagaagga ttgcacgatc ggcaggcatc acagcatatt cggcataacc
     6541 acctagattg actaatccgt atacttttgt cccaggagta aaagtggaga tatcactttt
     6601 attctcaact acctctccag ctacttcgac tcccaaaata gggtagggag gtttcaaaga
     6661 gactgattct cttttcataa tatctgttcg attgacagct gctgtatgga ctttgatcag
     6721 taattcgcct tgttttggtt taggaacagg cacttcaatt tgttctaata catctacaga
     6781 accaggatgc ttgattgaaa tggcacgcat ggatctattc ctcctttgcc cattcttgga
     6841 atgctgtatt caactctttg ctaccttcag aaagtactag agtcgtacga gaaatatccg
     6901 tttcttccac tagagcggca agtacagcag caactgtaga gcgagtgatc gtttttgatg
     6961 ccttctcatt aggcttagtt aattgaatgg aagtaacttc ttcatcgtta gtcaatgtga
     7021 ctggttgaag aatgacaaaa tctaagttag ttcgtttcag ccattcatct gcataatgtt
     7081 tagtaatata ataatctgtc atggactcag gccattttgc tggttcatct gcataaacag
     7141 cactgaccat cagatagcga gagacatgcg cattttcagc tgcgatcact gttttgattg
     7201 cgccatccaa gtctacttgc agaagattct ttccttgaga gccggcagca aaaatcaata
     7261 catcgatgcc tttgaatgct tcggtcattt tttccacgga ccaagttaaa tcaaaagaga
     7321 catacgaaac attctctttt tttactaatg tttgggaagc aacatctctc acccctgcaa
     7381 aaatttcatg ggaacttgga gctaaatctt ttatcaggtg tcggccaatt tgtccgtttg
     7441 cacctacaac aaaaattttc atttggatca tcctttcgac gtcaaatctt tccattcaat
     7501 agtaacacca aaagagaaaa aaatcagaga tcggctacga tgactatcag ttagtaagag
     7561 aagggtgctt gaagtatctt aacaagacat tgcatactgc tgcagtcgca agacctgcga
     7621 cgattccttc aggtatacca cttgtaaaaa taaccgctag aatagctgaa taaacagcat
     7681 ttaaatttgt tccgatgact ttggcataat cttgctggaa taaaaagtag atcatattca
     7741 tcactaaaat cgtattggtc aatgaaccgg aaagtccagc taagaataaa gaaacggaat
     7801 ctgctttttg tttcattagt ttgcgcagac ctttataaac gaaataagga accaccccaa
     7861 tcaagatgcg aggaataaac gcgatcagca aggctttcca gcttccatgt tctgttccga
     7921 tgactgggat aaacggagaa aagacaaaag acaaaggtgt ttggataact gtgctacgga
     7981 tcatgctgat cagtccaaat gtaccgccaa gaaaagcacc tagtcttggt cctaaaacga
     8041 tcgaagcaat gattacagga atatgcatgg ttgttgcatt gattggaccg atagggataa
     8101 aacctaaagg cgtaacagct aataaaatca aaatagctaa aaacatcgca gttaatgtaa
     8161 agttttttgt atttttcata agtactcctt ttttctaagc atttctttca ttaagagtca
     8221 gcatgacctg ttcgatgatc gtctgaatat cagctaaggc accttttcca aaatctccac
     8281 aagctaaaag agattctctt ggttcaattt cctgatagcc gacagatctc aatgtttcca
     8341 gattacgctg agtgatcggg ttttgataca tattcgtgtt catggcaggg gcaatcaatt
     8401 ttggtgttcc cactggaagc gccattgcga ctgttgaaag aagatcgtct gcaatgccgt
     8461 ttgctagctt gccgataatg tttgctgtag caggcgcgat gagaaacaag tcagcttgtt
     8521 ttgctagttc gatatgattg attacctgcg gatttgattc ttgcatgacg tctgtatgga
     8581 ctgcgcgctt cgacagagat tgcaaggtta atggcgtgat aaaatgtgtg ctgcttttag
     8641 acataatcac atctacctgg gcacctaatt tgacgaattg acttgtaaga tcagcgcttt
     8701 tgtaagcaga aatacttcca gtaacgccta gtaagatctt tttcattttg attcctccag
     8761 tctaattttc tcagtaatca atgcagcaat ctcagattta ctttgagctt cttccactgt
     8821 tccatcttta gataagagat agccgtggtg tgtctcgcca tggatttccg tcaaatcatt
     8881 tgcgaaaata aaatcagttt tgttatttat cagactattt tttgcgactg ccagaagaga
     8941 ttcctttggt acatctacta atagtttgaa accaatgatt tttgcttttg gctgccattc
     9001 acgtagataa gaaatgattt ttggtgtttt cttcaaaacg agcacaagat gctcggtgtt
     9061 agaagagatc tttgaaacag tggtatctgt ctgttcattt tccgcaaacc aattgtccag
     9121 ttcttcttgc gttgaagaag tgggtaggtt ttttgccagc tgttcttctg aaaaactaaa
     9181 agcaggagta aaatcactga ctgccatact gtggataatc gcatcgtagt gttctttttt
     9241 cgttagttct tctaattgta aaaataagtc cagtgcagat tcgatcgtat aaatcgttat
     9301 attggagtgt cttttcggtt tcagtgcttg tttcgtagtc acgtaatcga ttgtggtatc
     9361 cggattggca gctaaataat cagctaatgc ttgccctaat cgtcctgtcg aatgatttgt
     9421 aatagcacgt acttgatcga ttttttcagt cgttcctcca gctgtgatta gaatcttcat
     9481 ttatggtcac ctacatttac tagtgttctt ccttggtgtg ttccagcaag tagtttagat
     9541 gctgtttcag gcaattgatc cagagtaatc tcttggacga ccagttcatc tgcaatctgc
     9601 aaatccgcca tctgttgcca taaagaaaga cgttgatcta ttggtacatt gacagaatca
     9661 attccaatca actggatgtt tcgcagaata aaaggcaaga cagtcgcgtt tagtttgatt
     9721 cccccagcat tgccacataa agctactgca ccgttataag agatcaatgg aagaagactg
     9781 cttagctgct cgccacctac tgtgtcgatg acatagtcga tttgctgttt tcctaaagct
     9841 tttgttgtct ctggcagaaa ttcatcaggt cgtacgactt gcaaagcacc tagacttttt
     9901 agccatgtga cagactcttc ttttctggaa aaggcaatga ttgactggaa tcctagttta
     9961 tgtaacaggg ctatcgctgt actgcctaca ccgccagaag caccagtaac gacaactttt
    10021 ttatcggagg tcataccttg acggattaaa gcgttcacac acaacatagc agtaaaccct
    10081 gctgtaccaa ggatcatcgc ttgacgcagc gtcatatttt ttggtagagg aactaaccag
    10141 tctcctggta ctttttgata ttggctataa ccgcctggat gggtgacgcc aagcccaaaa
    10201 ccagtagcaa gtacaagatc ttcttttgaa aaacgtggat tgttactttg gacgacagtt
    10261 ccggataaat caatccctgg agtcatcgga tatgttcgta tcacctgacc agactcgctc
    10321 attgccaatg catctttata attgacatca gaataggcaa tttttaccaa gacttcattt
    10381 tcagccaact cagtaaatgg cagatcactg attgctgtat ggaaaacagg ttcctttatt
    10441 acctgaaaag atcgaaaagc gttcattcgt gagacactcc ttttaaaatt ttcttttatc
    10501 ataccataaa gcctactctt ttattcttgt tcttccgtaa atttagacaa aaattaggtg
    10561 aaaaataaag aatcaaacaa ctagaattta ttccaaaaga taaagaaata ttcagcaaaa
    10621 aaaattttac aaagggttgc ctttttcaat aaatttcgta tactaatacg agtggtagta
    10681 aacaggaagt ttactattag aaaacttcga ttcggaggaa attatggaaa ttggtgagaa
    10741 gttaagaaat ttgcgcatcc aaaagaattt gacacaagaa gaactaggcg aacgcacaga
    10801 tttgtcaaaa gggtatattt cccagttgga acgagattta agttcaccat caatggaaac
    10861 attcttttct attcttgaag tcttaggtgt gactccagaa gaattttttc atcaagaagc
    10921 agcgaatctg caagtggttt attcaaaaga agatcatacg gtttatttcg atgaagaaaa
    10981 cgggtatgaa ttggaatggc tcgtggctga ttcgaatgaa aaagaaatgg agcctgtctt
    11041 gctgactttt gaaggtgcag gagaatataa gacgtttgaa ccttcattat ctgaaacgtt
    11101 tatttttgta ttagaagggg aattagagtt aagcttagga gagacggtct atgtggcaaa
    11161 agcaggacag tcgatctatt atcaagcgac aaaacaacat caattaagga atcatctcaa
    11221 aaaacgaaca aaagtattga ttgttgcaac gcaatcatat ttgtaatata gggggtaaaa
    11281 acgtggggaa accaatcatt tcatttgatc aagtagtgaa acgctatgat gatgaaacag
    11341 tattaaaaaa agtaagtttt gagatcgaac aaggaaaatt ttatacactg cttggtcctt
    11401 ccggctgtgg taaaacaacg atcctacgta ttatagctgg attcaccgaa gctacagaag
    11461 gggacatcta ttttgaaggg aaacggttga atgatttgcc agctaataag cgacaagtaa
    11521 atacagtctt ccaagattat gcactttttc ctcatatgaa tgtttttgat aacgttgctt
    11581 ttggattgaa gatcaagaaa atgtccaaat cagatatcga aaagaaggta aaagatgctt
    11641 tgcgtatggt ccagcttcct ggatacgaaa aacgggagat tagtgaaatg tctggtggtc
    11701 aaagacagcg tgtagcgatt gcacgagcaa ttgtaaatga accgaaagta ttgctattag
    11761 atgaaccgtt atcggcgctt gatttgaaac tacgtacaga tatgcagtat gaactgcggg
    11821 atctgcaaca acgattagga atcacgttta tttttgttac tcatgaccaa gaagaagcgt
    11881 tggcaatgag tgatgagatt tttgtcatga ataaagggaa aatcgtccaa agtggtacac
    11941 ccgtagatat ttatgatgag ccaatcaatc atttcgttgc tgattttgtt ggggagagca
    12001 atatcgtgga cggtgtaatg atcgaagata atctagttga gtttgttggt aagcaatttg
    12061 aatgtgtcga tggcgggatg cgtctaaatg aaccagttga agttgtttta cgcccagagg
    12121 atttgacgat cacgacacca gataaaggaa aactagtcgt aacggtcgat actcagctgt
    12181 ttcgtggtgt ccattatgaa atcatctgtt atgatgaaca gcaaaatgaa tggatggtcc
    12241 attcaacgaa aaaagctaaa gaaggaagca aagtaggatt ggcctttgag ccagaagata
    12301 tccatgtcat gcgtttcaac gagtctgaag aagaattcga tgcacgtctt gacagctacg
    12361 aagaataggg gggacaagaa tgaaaacaat gcgcaggatc tattccgtcc cttatatttt
    12421 gtggctcggt ttattcgtga tcgcgcctgt attaatgatc atttatcaat ctttttttga
    12481 tatgaatggg caattcacgc tgaataatta tctatcttat tttacgtcgg ggacatcgct
    12541 ttctatgacg atcaattctg tatggtatgc atttttgatc acgtttttca cgctgatcat
    12601 cagttacccg actgcttatt ttttaacaaa actgaagcac aagcaattat ggctgatgct
    12661 gattattttg cctacatggg tgaatctgct gcttaaagct tacgcattta ttgggatttt
    12721 cagtgtgcac ggcagcgtga atcatttctt tgattttatc gggatcgggc cgaaacagat
    12781 attattcaca gacttcagtt ttctgttcgt ggcaacgtat atcgagattc catttatgat
    12841 tatgccaatt ttcaatgcat tggaagagat gaatccttct ttgatcagtg ctagtagaga
    12901 tttaggcgcg aatagctttg agaccttccg acgagtgatt tttccgttat cattaaatgg
    12961 tgtgaaaagt ggtgtccaag cagtatttat tccttctctt tctttattca tgctgactcg
    13021 tctgattggc ggaaatcggg tgatcacttt aggaacggct atcgaacagc atttcttggt
    13081 tacacaaaac tgggggatgg gttctacaat cggtgtgatt ttaatcattg ctatgttcat
    13141 cgtgatgctc ctgacaggcg agaagaaaaa aggaggtcgc tcataaatga aaaaatttaa
    13201 atggtcgtat ctttatttag ggatcgtctt tttattctta tatgcaccga tcttctatct
    13261 gattttttat tccttcaatg aaggaggaac gatgaacagc tttacaggat ttacttggga
    13321 aaattatgaa gctgtttttg aagatacacg tttgatcatt atcgtattga atacgttcat
    13381 gctggctttc ttatctgcat tgatcgccac gatcatcggg acatttgggg ctatgggaat
    13441 ctattacacg aaaaaacgac gtgcacggac agcgttgttg agcatgaata acatcttatt
    13501 agtctctcct gatgttatta tcggtgctag tttcttgatt ttctttacac tactaggatt
    13561 cggattaggt tttcagagtg tattgctttc ccatattgct ttcagcattc caatcgtcgt
    13621 cttgatggtc ttgccaaaat tgcaggaaat gaacgattca atggtcgatg cggctagaga
    13681 ccttggtgct aataatttcc aagtagtcaa aaatatcatt ttgccttatc tttctccagg
    13741 aatcatagct ggatatttta tggcatttac ctattctctt gatgattttg cggttacatt
    13801 tttcgtcaca ggaaatggct ttacgacgct ctcagtagag atctattctc gtgcaagaca
    13861 aggaatcagt ttagaaatca atgcgctaag tgcgttagtt ttcttgttct ccatgatatt
    13921 ggtcattgga tactacttta ttagtaaaga aaaccgtcca aaacggctga agaaaaacag
    13981 aagagtacgc gagggggtgg cacggctgcg atgaaaaaat tacaatctct gtttttaggt
    14041 attctcatca ttattgcggt gctgctgctt ggtgttcgtc aacttgaaaa aacaagtggc
    14101 atgagtggag caaaggtact taccatctat aactggggag attatatcga tcctagtttg
    14161 atcacgaaat ttgaaaaaga atatggatac aaagtgaatt atgaaacatt tgattctaat
    14221 gaagcgatgt tcacaaaaat ccagcaaggt gggacaaact acgacatcgc tataccaagt
    14281 gaatatatga tccaaaaaat gatcaaagaa aaactagtac ttccattaga tcattcgaag
    14341 atcaaaggat taaataatat tgatccacga tttttagact tggattttga tccagataat
    14401 cagtattcga ttccatattt ttggggaact ttagggatcg tatataacga taaattcttt
    14461 gatgcaaatc aaattaagca ttgggatgat ttgtggaagc cggaattaaa agacagtttg
    14521 atgctgattg acggtgcacg agaagttatg gggctttctt tgaatagttt aggctattct
    14581 ttgaatagta agaatatggc acagttgaca gaagcagcaa ataaattgaa tcatttaaca
    14641 ccaaatgtca aagcaatcgt ggcagatgag atcaaaatgt atatgatcaa tgaagaagcc
    14701 tccgtggcag tgacattttc tggcgaagct gccgatatga tgtgggaaaa tgaacattta
    14761 cattatgtga tcccaccaga aggatccaat ctttggtttg ataatatcgt tatgccaaaa
    14821 accgtgaaaa ataaagaagg tgcctatgat ttcatcaact ttatgctaga accggaaaat
    14881 gcggcacaaa atgcagagta tattggttat tctacaccaa atgaagaagc aaaagcgtta
    14941 ttgccaaaag acatttcgtc ggacgaacaa ttctatccat ctgatgacac gatctcccat
    15001 cttgaagtct acaaagatct aggatcgaaa tacctaggca tctataatga cctattctta
    15061 gaattcaaga tgtataggag ataatggcaa aactcagtaa gaatggggac ttatcaacgt
    15121 tctaaagagc taaaaatcgt cctttttcta tctgagtttt ccaatcttct accaaaagcg
    15181 gatgaaacgc cgcttttttt gtgttatttt attttttcta ccacgttttt ctaccagaga
    15241 aaagtggaca aaaccattgt aaaatagggc ccgagacggg ctagtaaatc taagattgaa
    15301 aaagttacag aaggataaag taattggaaa ctatgcaaat atacttaacc cttctgtaat
    15361 ctattaataa aactaatata tgaatccagt tcaatatttg tagctatgaa aaaacataaa
    15421 aatcatatta gataaaaact aaattggtag ataaattatt ttgtcagata tctgaggcaa
    15481 atattcgact atttgtgttt caaagtaaaa attggaatac aaatatagaa tagacatatt
    15541 gcagccgata gtttattatg cttttagaca cattaaactg gaggaaaaaa tgaaaaagag
    15601 cgctaaagat cgagtttttg atgagctgat acgactaaca gggaatatgt cttctgtcac
    15661 tgccgagaaa atttcgaaga atctaggtat ctctcgtcag aatgccagcc attatttgac
    15721 gagattagtg gaagataaaa aggtcgagaa attaccggga aagcctgttt tgtggaagcc
    15781 tttagatgaa tatgcggttg tagataatac ggagcaaata aacgaagcct ttcattctgt
    15841 agttggtcat gacggaagtc tgagagaggt cattcaaaaa tgtatagccg ctgtcaaata
    15901 tccgcctaac ggactcagtg tattaataaa tggagccacg ggtgttggaa aaagtttttt
    15961 agccacaaaa atttttgaat acgctatcca tgagcaaatt attgaaaaag atgcaccatt
    16021 tgctatctta aactgtgccg attatgcgga caatcccgaa ctgctttcgg caacactgtt
    16081 tggatacaag aagggggcct ttacgggggc tgaaaaggat accgatggtt tattggcaac
    16141 ggccaacaac ggctatcttt ttctcgatga ggtccatcgc ctatcgaaag aaaatcaaga
    16201 aaagctcttt ctatttattg atactggaaa ctatcgtcca gtcggtgaaa atgtaaattg
    16261 gcattctgct aaggttcgtt ttattttcgc cacgacggaa aaaggtgaaa attatttatt
    16321 agacaccttt gatcggcgta ttcaaattag tgttatgttg ccaacttttg aagatcgccc
    16381 cattcgagaa cgattagagc tgattcaatt attttttcaa aatgaagcgg atgttttaca
    16441 aaaagatatt attgtttcaa gagaagcctt gtctttaatg ttatctcatc cctttagtgg
    16501 gaatattggt aagctgaaga atatcattaa aatcagttgt gcggatgtgt atagtcgaac
    16561 acaagaagaa ccattaacga ttgggaaaaa tgagatcttg attcagttaa atatgctgga
    16621 atccgaagta gaaagcttgc cgattgctag tttattgata aagaaaaatc aaaagataaa
    16681 aacggatcga atcaaaccgt ttgattatga ttcgattttg gaaaaaatta ctcagctgat
    16741 aaatgaaggt aatttcaatg aaaatttttc ttcgataaag aatgaagtac agcatttggt
    16801 gcatcacatt gataaggatt cttttttatc aatgagtaga aaattgatga ttcagatgtt
    16861 tagaaaggtc tgggtatccg ttttaggaaa aacgtacgga ttgacgacga gtggacctgt
    16921 tgcagaaatt attagcagat cctatgtcat ttccccaagc tataagacag acttaaccgg
    16981 gataaatcgt acattagctg caaatctacc tcggacgact tacttatgta atcagttctt
    17041 ggagcattta ccaccgctct cagaaaagga cgagaacttt ctaagactgc ttattgtcgt
    17101 gactttatct gattatgtag atgaatccat cgagctgaag ggcctgttgc ttgctcacgg
    17161 agatagtacg gctagcagca ttcaagcagt agtgaatcaa ttatgcggca attatgtttt
    17221 tgaagcgttg gatatgccag tagagagtaa tgtgtcagag attgtcgaat cgacaaggaa
    17281 tttcgtgaaa cggcaagtga atacagatag tctagtgctg atcgtagata tgggctcttt
    17341 aaatcaaatt tacacacaga tcaagaacga tttaaaaggg gagttacttc ttctgaataa
    17401 tttgacgact tccattgcac ttgatattgg cttgaagatg acgatgaatg cgccatttaa
    17461 acagattgcg gaggatgcag cagacaagta ttcaatcaat gttcaatact ttgaaggctt
    17521 ttctcaaagt agcaacatta tcatttcctg tatgtcgggt ttggggattt cggacaaatt
    17581 gaaagatgtt tttcgtcatc atttatcgga tgaatccatc gaagttttca cgaaggacta
    17641 cagagaacta cgtgaattaa tcgaccaaaa tgatgaaact tattttgaca agacaaaatt
    17701 ggtcatcacg acttcggatc ttccacgttc cttttcaatt cctaatatta atgtttatga
    17761 catcttagac ggtgatggag cagccaattt ggagcatatt ttgagtcctg aaatgaagcc
    17821 ggcagaattt aaccgcttga ttcaggagct agtaaaattc ttttcgttgg aaggaattgc
    17881 ggaccgtttg aactttctga acccaacgat cgtgatgagt gaagtggaaa cagtcatttc
    17941 taaatatgaa aattactatc atttcacttt aaatggccgt gtaaaactaa atctctatat
    18001 gcatacggca ttgatgatgg aacgtttatt cttggctcgg acagaaaaat ctgaagaacc
    18061 gaattatgat ttgaaaccag atgaagatga attttattat gtttctagaa gtatttttca
    18121 atcaatggag atgaaatata attttaaagt caatgcttat gagctttcgc tgctctatga
    18181 attattagga ccgtatattc aaaaataaag tgtctaaaaa agtgtctaat ctgttttttt
    18241 taggttagac acttttttta atgtgaatta acgtgattaa accaatgttt ttaaaagttg
    18301 gcacaggaat tgcttatata aaagtgaaca gaaagaaagg aattaaccat atgaagaaaa
    18361 ttctaatagc gactcacggt cacttagctt caggctttct tagcagtatt caattactga
    18421 cggggaaggt tcaagagatt acggtgatta atgcttatgt ggatgagtgt gattttgaac
    18481 aggagttgaa tgtatttgta aaccaggtga acgagcagga tcagattttt gtttttaccg
    18541 atttatttgg cggaagtgtc aatcagaaga ttaccaaaac ctttttggag aaagagattg
    18601 ctgtaactat tttgtctggc tttaatctgc caattctgct cgaaatagta ttggcaacca
    18661 aggaattaac aaaggaggat attcaggaac tggtaaataa atgccaacag gaattaaaga
    18721 tgaccattct tgatgaggta catgtagtaa atgaagagga agattttttt taggaggaag
    18781 aaaaatgatt aaagcagtgc gaattgatga acgattaatt catggacaag tagcgatgac
    18841 ttggacaaag agtctaaatt taaccgggtt agtagtagca agcgatgagg cggctaccaa
    18901 cgatattcaa aagatgacat tgaagatggc tgcaccatcg aatgtcaaag tcattattaa
    18961 aacagttgat gacgcgattc atttgttaaa agatccgcgg gcgaaggaca tgcgcttgat
    19021 ggttttagtc cctaatgtca aagatgcggt aaaagttgcg aaagctttcc caacggaaat
    19081 cgaattaatg aacgtgggga atgctgggaa aatgacaccg ccatcggaag ataagaaaac
    19141 cttatcaaag gaagtgatgt taacgacgtc agaaattcaa gacttaaaag aattagttga
    19201 actttatcca gataccgttt ttcagcctac accggcgatg gaaaaacgtt cagctatctc
    19261 tattttgagt gatttttaga aaaaggaagg agaaaaatta tgtatgatgc atttgtagtc
    19321 gctttggccg tttttattgg aattgctgga catgaaatgt tcgggatgtc catgctgagt
    19381 cgtccgattg ttgttgctcc gttagcagga gcgttgatgg gggatttgca gacggggttg
    19441 attgtcgggg cgtcactaga agccatcttt atgggtgtcg tcaatatcgg aatctctagt
    19501 acggcggagc cttctttggc tgctgggctg gctacagcgt tcgcaatcaa aatgggagga
    19561 aacgtggatg cgattattcc cattgtcttt ccactagcag tattaggatt acagttaatg
    19621 aatatgattt tttcttttgt atgtggaccg atggctgcta aatttttcaa atttgcaaaa
    19681 tctggtgatc aaaaaggaat catcaatctg cacttcttta tgtggtttgt ccactatgcg
    19741 ctttatgctc ttattccatt ctttgccgtt ttatttggag cagatgttgt caaaagcgtc
    19801 ttggataata ttccagaggt catcatgaac ggattaacag tcgctggaaa tctgctgccg
    19861 gcagttggga tggcgatgct gttacggatg ctttggagta aaaatattgc tgtgtggttt
    19921 ttctttgggg ccgttgttat ggcgtatctc gagttaccat tgattgcaat tgccgcagtt
    19981 ggaacgatta ttgcaattgt tatagcccaa agagatctac aactaaaaaa tatatctgtt
    20041 catgcagttc aaagcgatgc tgctgtaaat gcaacgcttg accaagaaga ggaggatttc
    20101 tttcaatgaa attagctgat ggaatttcta aagatgagaa aaaattggtt cggaagatgt
    20161 tttggcgctc gatgactatg tatgcttcgg tgaatccggt gacgatgggc ggcggtggtt
    20221 tcgcctattc tatgatgccc ttcattaatc atttctacaa aagtgaagaa gatcgagctg
    20281 cggcgctgga acgtcataca cagtattttt cgacgaccat tccttgcgcg tcctttgtca
    20341 tggggattgc ggcatcgatg gagaaggaaa attctgaaaa agctggtttt gacgtggaaa
    20401 gtatcaacgc gatcaaaacg agtttgatgg gaccattggc tggaattggc gattcattgt
    20461 tctggggagt ttggcgtgtc gttaccgcgg gactagcgat caacttagcc aatgccggaa
    20521 atattttggc accaattatc tttttagtta tgttcaatat acctaattac ttgatgcgat
    20581 actatggcgg cgttttaggc tattcattag gctctaaata tattgaaaag ctgtatgcga
    20641 acgggttaat tgaaattctg acaaaagctg ctagtatctt gggtctgatc atggtaggtg
    20701 caatgacgat cagtatggtc aaattcaata ccgttttgag cttttcaatg aagggcgaag
    20761 aactaatgaa tgtccaagaa atcctcgata gtatttttaa aggaattgta ccgttattgg
    20821 taacattgag ctgtttcaaa ttactagata aaaaagtcgg cgtcatcaaa attatttttg
    20881 gaatggtcgt cttgggaatt ttattgtcat tacttggaat cgcatagtag gaagctgcag
    20941 ctactaggct gcagtttttt tagcaaggag ggaaaagatg actattaatg aagtaattta
    21001 ttcacccaaa cacccatttt ttaatgctga gttaaacgac ggaaatgtga ctacttcaaa
    21061 cgaatacagc tactatcgga atattcctga tgtaaaggga ttgaccttcg atgtaagtaa
    21121 tcaaaagaca ttagcaaacc ttgatgtttg cgggacgatt caaacgttga ccttttatcg
    21181 agaaaattta ctaactgagg aaaaaccagg tgtttgggtc aataaacagc tctctcaaac
    21241 caattcgtta tccttaaagg ttgaagtaaa cgggattgtc taccatttgt cagaatcgga
    21301 tcatcgtatt gaagtcgatg tgattcaaga ctgtttgcct agatatattc atcattattc
    21361 gacgtttaag gtcattgtag tatcctttgc tcccatactg gctaataagc gtttaagtat
    21421 gcttgttcaa caggtttaca ttgtgaatga aacacacgaa agccttcaag tttcagtgaa
    21481 agatgtgcct tcgtatcaag agaagtattc ggatcagcaa aatgtgttga tttctcaaaa
    21541 aggaaataag gacacgatcg cacaacagga agtggcaagc ttcgccattg cttttgtgga
    21601 cccaaatgct tttgaagaag taatgacttt tcaggaatct gatatcgaaa aatggctgaa
    21661 ggaaacattg aactattttg ctcagctatt tgggcagctg tcaatgccaa acgatagagt
    21721 tgtccatctc tatcgtcgtg cactttatca aagcttttct agtttcggta tgaatcgaca
    21781 gggacaggtc gtcggcagca attggggcag ctatcctgct actaatcgaa tttggaataa
    21841 ggatatgtat tacgcatcat tgccattcac catgtttgaa ccggagttat gtcaaaaaac
    21901 ggttctatgg tttgatcaat atggcgttaa gtttcctggt tcaaaattta caggaggtat
    21961 caatcattct ctgagtaatt cgttatccag tgcgttactg gctagtcttt attttgaaca
    22021 tactggggat acaaaattct ttgagcggtg tccgcaggtt ctactgaatg cttcaagaat
    22081 tattgaggac atattagctc aacgtcggtc aggggaacca atgctttttc catctgtttg
    22141 gttatcagac gcctttgcac ttggaaagta tcataccggc agcaatcttt gtctttggaa
    22201 ggcctgtatt ggacttgcag aaatttttga agccctagaa caactgagtc ttagcaagcg
    22261 atacaaaaac attgcctgta aactaaagga atcaatttgt aaacaaatga ctatcgatgg
    22321 tccttttggt gagcaatttt tggagggaat cggtgatgag gagaaaacaa cctattcagt
    22381 gaagcactat caaaaaccga ttcttgagca agggttaatc tttttaagtg atgtgattgt
    22441 ggatggaaaa attaatttat tgatgcatga tggagaagaa tcagacacta cgttgatgcc
    22501 tttttatcaa tttttagata ataaagatca tttatatcag aacaccatgg ccttttctgc
    22561 tagttctttc aatccaacct atagcgagga aatcaaaggc atcacttggg gattagaatc
    22621 cggcgccact tttcctggct ttatcactgt tttaatgagc gagctgcaca accctcaagc
    22681 tttcgtaacc cgattagaag aactaatttg tttggcagat ttagacggtt cgtggtggtg
    22741 gtggccgtat cgcttagagg ctcaacaagg agaagtcgtg cgagactttg gctgcggcaa
    22801 atgcggctgg gcatcgggtc tttttgtcag cctaatgatc agtcaatatt tcggattgaa
    22861 attcaaaaaa ggagtattgc aaattgatcc tgttgataat ttaacgtttt cttggaagaa
    22921 tttgaagttc ggtcaagcct tcatttcgat tgaatgtacg caatccgagt tgagtatttc
    22981 aaatttgtca gaaaagccat tgaaaataag tgatacgaaa aaaattcttc acgtagaaaa
    23041 gggaaaaacg attcacattc aaaggagaaa aagatgagta tactaaaaaa tattacccaa
    23101 tttaaaaaaa tccagagtcg agcaatcact cccgaaaatc caacaggcga gaaaggtaaa
    23161 gctgcgatgt tgccaagcga gctaggacca agccgcaaag gtcgtggcgc aattccgctg
    23221 acttctggtg aagaaactgt gattgcggat attactggaa cgggaataat tcgtcatatg
    23281 tggatgacga ttcgtgatcg aacagaattt ggcagttttg ttttacgtga tttaatctta
    23341 agaatttatt gggatgggtc ggatagccct gctgttgaaa gtcctttagg ggatttcttt
    23401 tgtaatgggt ttggagagcg ttgcgaggtg aactcgttgc cgatagtggt taatcctaca
    23461 gggggcttta attcctattt cgaaatgcct ttccaaacat ccgctaaaat tacgatcgag
    23521 aatttacatc cgaaggatat gtcctcattt ttttatacga taaattacgc tttggtggac
    23581 gaattggaag aaaatagcct atattttcac gcaaattggc atcgggaacg cattaccaaa
    23641 ttacaggagg attataagtt aattgatcga ttaaagggtc aaggctatta cgtgggtact
    23701 taccttgcgc ttacctgcct agaacgttat tggtggggcg aaggcgagtt taaattctac
    23761 attgatgggg atagtgagta tccgaccgta acctcaacag gttcagaaga ttattttggc
    23821 ggggcgtggg cctttcatga gtacgatgag ttcggacgtc cacatgcgaa aaactattca
    23881 acagcctttt tagggtatcc ttaccaatca aatagagatg ccacaagaga tcgttttgaa
    23941 acgggaaaat tgaatgccgt tcatgccttt ggtgacgacg caatgccacg acatggtctt
    24001 tatcgttggc atcttcaaga ccccatctgt tttaccgaag atttatcggt cactttacaa
    24061 caaatcggca acgatgatgt tcgcttattt gagcggcaag acgatgttgc tagtgtcgct
    24121 tactggtatt caacagaagc ttcaggaaat actaaacgtc atttcactga tcgagaacgc
    24181 ttgccgcgtt aatgaagtgt ttttcacata ttcgaaggga ggaacctatg atgatatatg
    24241 gggcaattga agctggaggg actaaatttg tctgtgcagt cggtactgag gaattaacga
    24301 ttttggaaag agtcagtttt cccacaacaa ctccagttga aacaatgaag caggtcattg
    24361 atttttttaa accttttaag gaacaattgg caggcatcgg ggtcggctgc tttggtccaa
    24421 ttgatattca atgcaaatca acgagtttcg gtcatatcac atcaacacca aaacttgctt
    24481 ggcaaaattt caattttgta ggaacgctaa agcaatattt caatattccg attgaatgga
    24541 caacagatgt caatgcggct tgctatggtg aatatgttgc tggttctggc aaagggttag
    24601 caggtgttgc atacttcact gtgggaacag gtatcggagg tggtgccttg ttcaatggga
    24661 ctttcgtgga agggttcagt catccggaaa tggggcatat gttgatcaag cgacatccaa
    24721 aggacaattt ttcaggaaac tgtcccttcc accaagattg tttagagggg atggcagcag
    24781 gaccagcgat tgaaaaacgg ttgggtgtaa aaggccagaa tttattggct gacgattcgt
    24841 tttggcagat tgaagccttt taccttgccc aatgtgcata caatacaaca ctgatgtttt
    24901 cgccagatag aattattttt ggcggtggtg taatgaaaca agaacacatg aagaaaaaag
    24961 ttcaggataa atttgttgag cttataaatg gatatgttga aataccacca attgatagct
    25021 acattattac gcccgaatta ggtgataatg ccggaataat cggcggatta gcattggcca
    25081 gaaaagcggt aagaaataag caaccataat taatgaatgt ttcattgata ctcatgtcaa
    25141 aagtatttaa ccgagtaatc aaatgatcaa atgaagttgg ataaagcgaa aatgaattca
    25201 aataaaaagg ctctaagtca aatcgtgttg ttttcattaa atcactgaaa ggaaatgctt
    25261 ctgcgtttcc ttttttcttt ttgttgtgcg ctgtatacct tgaggatatt cactggtggc
    25321 gtacacttaa atagtaatat attgtgttac tttatgtctg tggtaatagt tatagttgct
    25381 taccaatgta tgttcatttt tcgaaatgtt gacttcataa tccttggctt aatgtttgaa
    25441 tttttttggt gatagat
//
LOCUS       DACDSY010000022        24919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDSY010000022 DACDSY010000000
VERSION     DACDSY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.022"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(368..1486)
                     /locus_tag="IWK44_000799"
     CDS             complement(368..1486)
                     /locus_tag="IWK44_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549351.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..3264
                     /locus_tag="IWK44_000800"
     CDS             1957..3264
                     /locus_tag="IWK44_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP9549352.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3383..4582
                     /locus_tag="IWK44_000801"
     CDS             3383..4582
                     /locus_tag="IWK44_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9549353.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4609..5877)
                     /locus_tag="IWK44_000802"
     CDS             complement(4609..5877)
                     /locus_tag="IWK44_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP9549354.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6040..6686)
                     /locus_tag="IWK44_000803"
                     /pseudo
     CDS             complement(6040..6686)
                     /locus_tag="IWK44_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7094..7597)
                     /locus_tag="IWK44_000804"
     CDS             complement(7094..7597)
                     /locus_tag="IWK44_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9549355.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7720..8484)
                     /locus_tag="IWK44_000805"
     CDS             complement(7720..8484)
                     /locus_tag="IWK44_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP9549356.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8591..8866
                     /locus_tag="IWK44_000806"
     CDS             8591..8866
                     /locus_tag="IWK44_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP9549357.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9582..10532
                     /gene="yidC"
                     /locus_tag="IWK44_000807"
     CDS             9582..10532
                     /gene="yidC"
                     /locus_tag="IWK44_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9549358.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGGAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGRQQKRK"
     gene            10654..11863
                     /locus_tag="IWK44_000808"
                     /pseudo
     CDS             10654..11863
                     /locus_tag="IWK44_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466553.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(12173..12394)
                     /locus_tag="IWK44_000809"
     CDS             complement(12173..12394)
                     /locus_tag="IWK44_000809"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9549359.1"
                     /translation="MFKNYTINQKILPLDMEQYISETDVAFAVNALVEEKIKVDLYVS
                     SKFTNVKVVGADLFVVNVRKQKVSETDKF"
     gene            complement(12555..13076)
                     /gene="tnpA"
                     /locus_tag="IWK44_000810"
                     /pseudo
     CDS             complement(12555..13076)
                     /gene="tnpA"
                     /locus_tag="IWK44_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631987.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(13167..13533)
                     /locus_tag="IWK44_000811"
                     /pseudo
     CDS             complement(13167..13533)
                     /locus_tag="IWK44_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
     gene            complement(13547..13832)
                     /locus_tag="IWK44_000812"
                     /pseudo
     CDS             complement(13547..13832)
                     /locus_tag="IWK44_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(13838..14032)
                     /locus_tag="IWK44_000813"
     CDS             complement(13838..14032)
                     /locus_tag="IWK44_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549360.1"
                     /translation="MAETRPKGYPKLKEYTPSRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            complement(14032..14508)
                     /locus_tag="IWK44_000814"
                     /pseudo
     CDS             complement(14032..14508)
                     /locus_tag="IWK44_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            complement(14976..15329)
                     /locus_tag="IWK44_000815"
     CDS             complement(14976..15329)
                     /locus_tag="IWK44_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549361.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            complement(15457..15996)
                     /locus_tag="IWK44_000816"
     CDS             complement(15457..15996)
                     /locus_tag="IWK44_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549362.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGTDVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            16026..16217
                     /locus_tag="IWK44_000817"
     CDS             16026..16217
                     /locus_tag="IWK44_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549363.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCEKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            complement(16259..17125)
                     /locus_tag="IWK44_000818"
     CDS             complement(16259..17125)
                     /locus_tag="IWK44_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9549364.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(17149..18801)
                     /locus_tag="IWK44_000819"
     CDS             complement(17149..18801)
                     /locus_tag="IWK44_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9549365.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            complement(18806..19708)
                     /locus_tag="IWK44_000820"
     CDS             complement(18806..19708)
                     /locus_tag="IWK44_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAP9549366.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSISFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            complement(19792..21105)
                     /locus_tag="IWK44_000821"
     CDS             complement(19792..21105)
                     /locus_tag="IWK44_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP9549367.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVICASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            complement(21173..22117)
                     /locus_tag="IWK44_000822"
     CDS             complement(21173..22117)
                     /locus_tag="IWK44_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAP9549368.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWENGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            complement(22154..23167)
                     /locus_tag="IWK44_000823"
     CDS             complement(22154..23167)
                     /locus_tag="IWK44_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549369.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            complement(23247..24293)
                     /gene="gmd"
                     /locus_tag="IWK44_000824"
     CDS             complement(23247..24293)
                     /gene="gmd"
                     /locus_tag="IWK44_000824"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAP9549370.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
     gene            complement(24565..>24919)
                     /locus_tag="IWK44_000825"
     CDS             complement(24565..>24919)
                     /locus_tag="IWK44_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9549371.1"
                     /translation="EVEIFHTDRGKEFDNQAIDELSTTFDINRSLSHKGCPFDNAVAE
                     STYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNRLRLHGTLGYETPVGYRNQRLAQR
                     ILDNELGCANASEAV"
BASE COUNT         7089 a         5641 c         4744 g         7445 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcaacactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc gttatactgt
     3361 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
     3421 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
     3481 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
     3541 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
     3601 ttatttcaca gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag
     3661 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact
     3721 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
     3781 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
     3841 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
     3901 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
     3961 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
     4021 gcctcgatac aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac
     4081 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
     4141 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
     4201 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga
     4261 cattcttgtc gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat
     4321 catggatcag caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat
     4381 ttttagagcc ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa
     4441 aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat
     4501 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
     4561 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
     4621 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
     4681 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
     4741 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
     4801 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
     4861 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
     4921 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
     4981 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
     5041 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
     5101 atttcgacaa acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga
     5161 acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
     5221 cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata
     5281 gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
     5341 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
     5401 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt
     5461 atccaatcgg aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt
     5521 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
     5581 tggaataagc cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
     5641 gtttccacga ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
     5701 aactggtagt agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
     5761 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
     5821 cagccacttc agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa
     5881 aaattcctcc tcaataaaaa aacgcctact aaagaaaggg gctgtcccca gactttagta
     5941 ggcgtcatta gctatctttc gttagcattt aaaggcgaac accatcacct attcacttcc
     6001 attaaaagat agcatatttt tctgtaaaaa acaataaaat tatcgttctt tctttacacg
     6061 ttcagcaaat tcatagaaag gagccaattt cgttaactgt tgattacatt ggtcactatc
     6121 atgacaaata taattcccct ttttcgtata cgtgccatca gaacctgctt tcgttgtaga
     6181 aagaaaaagc gaaacattcg atgttttctg acagattgca cagattccct tatttatcgt
     6241 tggtgataaa gtaccgctaa tcccttttaa tttttcatct tcgtaataga ccatgaattt
     6301 tttctgcgta cctggatcat tccagccgat gtacgtatgt tcttgcaagt cgatttcatt
     6361 ccaagctggt tgtttgagtt ttttcacttt tcggaataat ttctcgattt gtggtttgct
     6421 aggctgcata aacggctgaa cgatttgctt cagctcctcc aatgctcttt ctgcttgcgc
     6481 ttttgtcagt tttacgtcta aaactgtcgt taaaaatgta tcgattctgg cagttcgtga
     6541 tctaatatgt ctcgaatctt ttcagctgct agtgcctgaa ctgtttgtac cgtttttgga
     6601 tcattgacag atgagtacgc atttaataac tgttctactt ctcttattat aaaaaaatat
     6661 tggtatggtt ttattgtttg ttccatgatt ttaaagtcct ttcttctaac caaagtgcat
     6721 actttgtaga aaaaagtgat cttctgcaaa gtatgtgtta cctgaagatc ctggactctt
     6781 ttaaataaga tacgttttgc atcttatcat cacccccttt aatgcttatt attctatgta
     6841 catgattcat tcgataaata aaagtaaacc gtatcttgaa cagatagtca actgcaaaca
     6901 gaaaaaagga ctgtgacaaa agtcgtgtca aagtcccttg tttcgaatga atggtgttca
     6961 aaagcttgca tctgcggtaa taagccgaaa cttcacaaaa atttgagaag taattttcgt
     7021 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcaata tggtctatta
     7081 ttggttttac ctgttaaatc gaggattctt tcccaaagta acgttgccat ctcttacgca
     7141 tacttgcaga catgggctga gccgcttctt tgactgcgca gtatttgtcc acgaatgtca
     7201 caatatatcc ttctttatac ttaggcggtg cgatcgttgc accccacatg tgcttcaaaa
     7261 tgatatctcg ctcaagatcc gacaaatcgg tgattttttc tgcattcttt acagcaattc
     7321 ttggatggat atacgcatgt gtcccctcgt caaatttcgt cgtacgccaa tcatagtaga
     7381 acagatcatg caaaagtcca gcacgcgctg ttgctttagc attgccaccc catttttttg
     7441 cgagcagata actatagtaa gaaacactga tggaatgctc caaacgagtg gaatgaacat
     7501 gctgtgtgta attcgctaat tttttcactg cttctgtttc taaaagatct tgtacataag
     7561 acatatattc ttcgtcttca cgccattttt ctgtcataat tatcgatttg ccaccttttt
     7621 gaatttcatt ttcttctgaa attgatctaa gtataccacc agtgttcgga ttattctagt
     7681 cgctctagat aaaatagcta aagcttaata gttccttaat cataaagaaa aatgataaag
     7741 aacgatccct gctgcgactc cgacattcaa tgattccgca tttcctttca ttgggatata
     7801 tacgttctga tcagtcgaag acaagatttc ctgacttact ccctgacctt cgtttcccat
     7861 gattatagcg aaatcctctg ttttttctac tttgtctaat gcatgtgctt cttcatttaa
     7921 ttctgtaccg tacaccgtaa accctttgcc tttcattaca gggatcactt ctgccaaatc
     7981 tttttgtaac acaggcaaat gataattgct tccctgcatg cttcgcagca cctttgtact
     8041 ataaatatct gccgttcctt tacctaataa aacaccagcc ataccaaacg catctgccgt
     8101 acggatcatc gttcccacat ttccaggatc ctgtacatta tctagtaaaa gccagcctcc
     8161 tgaaaaatct ggcgtatctt cagatgttaa cgtcatcact gctaagatcc cttgcggtgt
     8221 aggtagatct gacatagcgg tcatgacctc atctgaaact agaaaagtag aaatctcttt
     8281 gtttacagcc agccagtctc cccattcttt catacctctc tgtgtcacaa aaagttcttt
     8341 tatttctgca tcagacgacg cagcttcttc gatcagatgg aacccttcca aaagatattc
     8401 tgccttttct tctctgtatt tctttttatg tagttttttt aattctttga ttcttggatt
     8461 ttttactgat tggatctctt tcacattcct cacctcaact gattatatca tggaataggt
     8521 gcttgagctt gtttcatcct aaaaaaatcg gtatcattga tttataaaac aggaaagaaa
     8581 ggtgacgaac atgcgcaaag ttaaaatgaa tgtccaagga cgtgtccagg gcgtgggatt
     8641 tcgctatatg accaagatgg ttgctgatca gttaggcgtg accggaacgg taaaaaacga
     8701 agacgacggc tcagtcacaa tcgaagcaat cggaaatgac gacatcatcc aaaaatttat
     8761 cgaagaagtc aagaagtctc cgagcccaag tggacgagtg cagtatgtgg atgtccaaga
     8821 agatccgatg attgaggaac gaaagaaatt tgatgtggtg ggataaggtt gtttttgaag
     8881 cgctttgcct cgagtattaa ggagaaacat tttccaaata gcttctcata ttttgaaatg
     8941 taatgactta ataccgagag gcagctgata aaacaccgtc aataaggttg tgaaaaagct
     9001 ggtctgcatc aagcattaag gactggttgt gaaaaatagc tctccatatt tttcaacagg
     9061 ctggacttaa tgccgtagat gcaagctttt gaacatcgtc gataaggttg cgaaaaagct
     9121 gatctgcatc aagcattaag gactggttgt gaaaaagtcg cttagttttg agtattaagg
     9181 aaaattttgt gaaaatggct ttccacattt ttgcataaat ttgacttaat accaagaaac
     9241 tgacttttga acatcgtcga taaggttgtt tttgaggcac tttgcctcaa gtattaaaga
     9301 caggtagtaa aaaagctgat ctataacttg caataaattt gcttaaccca tattttccag
     9361 acagaacaaa aaagttttta gttttttaaa taagaaagaa acacttggaa tcactctttt
     9421 ctttctaagt atttctttct tgattttttg tcttttttca aaatttttcg ctacgaaaaa
     9481 gaacgaatat ttatatgaaa tttctatgaa gttgattgaa ttccatgcgc ttttttagta
     9541 tagttaagtt tgtgtaaaaa ttatagaaga ggaagtattc tatgaataaa tggaaaaact
     9601 ggttattggg atccggactt gtcagtatcc tgctcttttt atctggctgt gtgcggatgg
     9661 attcaaacgg aaaccctgac acttcaggaa tcgtttatcg agtactcgtc catcctatgg
     9721 gccaagcgat tacatatcta gttgagaatt tcaattggtc ttacggctgg gcagttattg
     9781 caatgactgt gatcgttcgt atcatcatct tgccactagg tatcagtcaa tcgaaaaaaa
     9841 caatgatcca atctgaaaaa atgcaagcgt taaaaccaca agttgaagca gcacaacaaa
     9901 agctaaaagc tgcaacaaca cgtgaagaac aaatggcagc acaagcagaa atgcagcaag
     9961 tatatcgcga aaatggattg agtatgaccg gcggaatcgg ctgtctgcct ctattgatcc
    10021 aaatgccgat cttctctgcc ttgtatttta cggctcgcta cacagaaggt atccgtgaat
    10081 catcttttta cggcatcgat ctaggctcac caagtatggt gcttgtggcc atcgcagggg
    10141 gcgcttatct gcttcaagga tatatttcaa caatcggtat tcctgaagaa cagaaaaaga
    10201 cgatgcgtac gatgctgatc gtctctcctg ctatgatcgt cttcatgtcg atcagtgcgc
    10261 cagccggtgt tacactttac tgggtagtcg gtggtgtgtt cagctgtctg caaacattca
    10321 tcacaaacgt tataatgaaa ccgcgtttga aagcacaagt ggcagaagag ttgaaacaaa
    10381 acccgccaaa acaagtcgtt actccaagaa aagacgtgac agaatcagca aagccagctg
    10441 acaacaaaca agcaacaaaa caaatcaaac aaccaaaaaa cagtacaaac aacaaaggcc
    10501 gcaacgcagg aaggcagcag aagcggaaat aaaaatatat gacaaaaaac tgtagacaaa
    10561 taaaaaaatt tctttgttct acagtttttt tgttacaatt aaagaaaacg ttcgcatttc
    10621 gaggaggaat gtataatgat gtccaaataa tcaatgtata gtcgaatgga agaagacaaa
    10681 atttcgctag atgcacttgt tcctaaaaat catttagtcc gtaaaattga caaattattg
    10741 tcatttgatt ttatttatct cattgttgaa tcaacttact cgaccattgg tcgccctagc
    10801 gttgatcctg tcgtcttagt aaaattagta ttcattcaat atttatttgg aatttgttcg
    10861 atgcgtcaga ccattaaata agttgaaaca aacctagctt atcgttggtt tcttgggctt
    10921 agtttaattg ataaagttcc tcatttttca acttttggta aaaactatgc acggcgtttc
    10981 agagaaaccg atttgtttga acagatattt atcattattc tcgaataagc agttgatgct
    11041 agatttattc atgaagaaat tttgtatatg gattcaactc atattaaagc gaatgcgaac
    11101 aaacgtattt ttacaagaga tatggcacat aaagaagcca ttttatacca aaaacaatta
    11161 gaagaagaaa tcaatgaggc aagaattgcc gaaggcaaac gtccttttgg ttcaatcatt
    11221 cgtcaggagg tggtcgaaag aaaatttagc atcgcagatc cagaaagtgg ttattatgta
    11281 aaaacagaac gagaaaaaca gtttgcttat tctgctcata ccgttggtga tgaaaatggt
    11341 tttgtattag atgtcatgat tacgcctgga aatctacatg atagccgaat gctcgtgcca
    11401 caaatcgaac gcgttaaatc acgttatggt gtagctgcta atgctgcgta caaaacacct
    11461 tggaacgtca agtatttaat cgaacgaaaa ctacgaccca ttctccctta tatcagtttc
    11521 gcacaactac tcgagatggc tataaaaaat atgtatctaa atcttttatt tgtgaaagtt
    11581 gtccattatt gaaaaattgt acagaaagcc aaaaacacca aaaagaaata catcgacata
    11641 tttgggaaga ctacttagat gaagcagaac atttacgcca tacggtgtac aatcgtgaaa
    11701 aatataaaaa aagaaaagaa acgattgaac gcatttttgc agacgcaaag gaaaaacatg
    11761 gaatgcgcta caccaagtat cgaggcttgg aaaaaatcag gtgcatacaa tgcttgtttt
    11821 tgtcgcaatg aatctgaaaa aattagctac ttggcagtgg gagcatatgt cgttatatct
    11881 tttattatac aaaaatagac aaaagtatca gtctacactc ttagtaaaga ggaaactgat
    11941 gcttttgtct acagtcttag agagtgggac aatagttttt tcttctttac gtcttttcct
    12001 ctccatgtat tttcctaatt ttcttaattt aacgccatga gtgccaagcc aatgtcaatc
    12061 ttggctcctt gtttccttca taagtggaaa tccgtttccg atagatcgaa ccttcttttt
    12121 cctctaaaag tttttttaca ttctgctttg aaataacgcc atgtattatt cactaaaatt
    12181 tgtcggtttc gctcactttt tgctttcgta cattgactac gaacaggtca gcaccgacca
    12241 ctttcacatt cgtaaatttt gaagacacgt ataaatccac ttttatcttt tcttcgacta
    12301 aagcgttgac cgcaaaagcg acatctgttt cagaaatata ttgttccata tctaatggta
    12361 aaattttttg gttgatggta taatttttaa acataaagca ctcgcttcct ttctgattgg
    12421 gtttggtcac tttaatccta cagggatgag tgctttttgt gtaccttaaa atcagtaaat
    12481 atccatccgt gaaaacgggt ggctttttaa cagccctaga agggctcacc actcacagcc
    12541 ctatctctct ttaactattt aaatggatct acatactctt gtttacttat actgtctcta
    12601 attcgatcat ctgcttcttg ttctcaaata tatatttcaa ttgttttttg atttaaccca
    12661 accgtactca cgtagtatcc tttggtccaa aagcttttat tcccatactt atatttcaaa
    12721 tttgcatgtt tttcatggat taaggttgca cttcgtcctt ttaagtagcc cataaaacta
    12781 gaaatactaa tctttggcgg tattcttact aacatatgaa tgtgatcagg cattgcatga
    12841 gcttcaatga tttcaacatc tttcctctca cacaattttc ttaatatctg gccaatactt
    12901 actcttaact ttccataaat cacttttcgt cgatacttcg gggtaaagac tatgtgatac
    12961 ttacaatccc atctcgtatg tgctaaactt ttatcgctaa agctatttcg tccacgcgta
    13021 gaaacgcgcg tttttttgtt tcgtacgcta cgccaacgaa actgactaaa gtcaataaaa
    13081 aaagtggggc aataatcaga tttttttctg actattgtcc cactctcttt ttattactct
    13141 tcatcttcta ctattccact aattaattag ttgacagata caatgctttc acatcctgca
    13201 cgaacaaact cgggctctga accaatcact tgcgagttga taaattcaat gaccatagga
    13261 agattaatcc gacataaagt ttaatctgtt gtccttccat tatcagttta gaaatgatat
    13321 tagtaggtgt accgcctaaa agatcgcata gtaccacaaa atcatttaac gaatctatga
    13381 cagtaagagc ttttgtctgt atgtttcagt gccatcttct ggtaataaag gtaacgtgta
    13441 gatattctct tgaggtccca taatcatttt tgtacttttt ttcaattcct cacaaaattt
    13501 tccgtgacta atcaatatca attttctact catttttggc tctcccttat ttcgaaatca
    13561 cacacaatgc agataaagcc acacacaagt aatacgataa aaatcgctcg agttgggtca
    13621 tatttttctt tcctaacaac caataaacac ctcgacgatc ccagcgagta agattttaga
    13681 taatatcata tcgatatagt tttgcatatt caatgtcaca tcgccaatcg ttggtaccat
    13741 aaaaatttaa tatcaatcat tgttgcaact aatgctccta ccatgaacac tcttaataaa
    13801 gtcgctacgt ccgttaatac agttaagcga gattacacta gatgatctgt tcctgccaag
    13861 gcaagagcca gaagcggttg cctgcccatt tgcctttggt gtgtcggagc tgtccgataa
    13921 agttcaccgc ccgatccgct ctggctttgt cgtagtgaca ttccgaaagc atgaatcggc
    13981 ttggtgtgta ttcctttaat ttgggatagc cctttggtct ggtttctgcc atcagctacc
    14041 acctcccaac agagcctcca tcgcatcttc tgctccgaca ccaccgccat caccggcaat
    14101 gatacggctt ctggacgaag gcgtcagacc gaactgctcg gaaaatcggt tcataatttt
    14161 cagataggtc tgggcaataa acacctgtgg tacctgctgc caatagccgg atagggtttt
    14221 cacgatgata ccgtgctggg taatgaattc ctcggcttcc ttccagcggg cgtaggcttg
    14281 gcactaaccg gcaaaggcag ccatatccac atctgtcaag attccgatgg cctccatctg
    14341 ctttgccaga cgcttccatt ccttctttgc ctctggttcc agccacttcg gacaggtcgg
    14401 tgccttcttt tccggcgttg actcttttgc attgagtggg cgattgcccg gatttccctc
    14461 cagcttcttc atggctgtcg gggttgattt tcttcctctg gttgccacag tcgtcacctc
    14521 ccttcaatgt atttctgcca gtagacagag aacagagcca agtcgaaacc tgactctctt
    14581 ctctatctgc taacttgtat ttcagtccac tcttctgatg aatcgcaccc gcaggatttt
    14641 caagatgccg caggcaaatt ccaagaagtt tctgaccgca tcataaccat cgtagccgtg
    14701 tagccacatc cttccacctc gccgccgtcc tcgtcgctga tataaatgat tctgtattcg
    14761 ctcatctgtt gttttctcca gtcttttgtt tttttgtagt acatatatca ctctaaaagt
    14821 gattattatc aagtcgtttg aaagccataa tgtacacaat cattcgactg gtttttggtt
    14881 cataattgac cgccaaagtg agcaaaagaa agggacctct gtctgtttca gaagtccctt
    14941 tcggtatttg gttcttagtg gttcaggctg tattctcaaa cctcatactc atcttcgagg
    15001 tctgcctctg caatgatctg gccgatgctg tcgtaacgct ggacctgcat ctggatggca
    15061 tccttgtctg ctccctcgtc catcatcttc agcagcttgt ctgattcctt tttgtaggcc
    15121 cagaaaagga tctcctgaag gctggtctcc tgctttttct tgcgaagggc gaccttcttc
    15181 tcttccaccg cctcgttcaa cttggtgaac tctgcccaag caaggatacc gtctcgcttg
    15241 attgcgatct ccacatcctt cagctccggg atggtctttg cacccttgat ctccttcaaa
    15301 tatttctttg ccttttcgct cattgccatt agcgtttcct ccttgttttt gggattttcc
    15361 cctttggttg tgtgtatatt gccatagtct ctcgactata tcaagtcaat ccacaggcat
    15421 atactacaca atcatttgga gctgtatttg tatacattat gccgatttat gagagaagaa
    15481 aaggctccag ccgttcgact ggaaccttgg gggggtcggt ttcttaggcg gcgtggcggt
    15541 gtagaccttg aggtagctga tgttcttcca gacgtccgtg cccttcagtc tgaccatgcc
    15601 gtccagctct cggtcgaggg tgaacttgcg gaaaagcttg atgcggttga ggctgtttgc
    15661 tagctccttg ccgttcttgg catccagaac tgcgtcctcg atctccgcaa tactaatttc
    15721 ctgtctaaac caaaggttgc tcaaggcttc gggaatgccg tttgcggtct gcagggggtt
    15781 gaaagtttcc aatacagcag gggtcgaaac cacctgtccg agctggaatt ttgcgttccc
    15841 taaaatcatc tgcataattt cttccatgtg aatcatccat ttctgccgtt gtggtattcc
    15901 ctttggcagt acagtactat atatcactct gaatcgtaca tttatcaaga agcggatttt
    15961 tgtggaatca ccaattcgat ataatagtaa ttgcatgaaa aatctaagca ctggattggt
    16021 aggtgatgat tatacgtgac gatcacattt acacctgtga ttcctgccac tacagcttcc
    16081 ctgccgacga acagccagag cgttgtcctg actgcgaaaa gacagcaact cggctggata
    16141 ccgaaatcga aactgaagat tactatcgtg ttcgtgctga aatcaaagct gagattaaag
    16201 ctttgaatgc gggttaacga caggtactga ccggcggctc aacaccgccg gctttttatt
    16261 accacatctt gcggaattcg ctgggacgaa caccttccac cttgaaaaaa gttctggaaa
    16321 aatagtttgc atctgttatg ccaatttttg ttgcaatctc ttccatactt agatttgtaa
    16381 agcgtagcaa gtgcttggcc tgtgtgattc gtttctgatt cagataaccg acaacagttg
    16441 taccgtaagt ttcacgaaaa atcttagcta agtactgttt attgataaaa aactctgcac
    16501 tcaactgatc cagcgtaatc ttctccgtgt aatggtcatc cagatattgt tttatgttct
    16561 gcaattccat tttttttgta gagaaattcg gcactttgtc aggattccaa gaatcttcca
    16621 tcaaatgcat cagcagttca ctgagtagaa cattgatttt catatcccgg acatactcct
    16681 gtccctctgc caccatatga aggctcctca gcaagtcagc atacttatct gtttttgatg
    16741 ttttaaagac cggttgacca cctcgttcaa ggtatttttt gtagacttcc gtcataccaa
    16801 tgccattaaa atggcaccac cgcaaagtcc acaaatcatc ttctgatgac tcatggacat
    16861 acggcttcaa acagtcaata aacaccatat ctcctacttg aagcgtatat ttctgcccct
    16921 tatactccaa tgtgcctgaa ccaacttcta ccaggaaaca aaggtaagat gacaatcctg
    16981 tacgcctaga tgtatgtgcc ttcagagcag tcagctgtcc aatttcctgc aaatacaaaa
    17041 gagaggatct tgcaaagaac gatgccgtgt acagcacacg atttgacttc accgatttca
    17101 aagcgttaga aaacagttcc tccattacaa gatttctcct ttcttttctt actcgaacat
    17161 ttccgccagt tcagcagcca taatagcttc catagcttcg gcggcttcct tgttctctgc
    17221 gctgacgctg atataggtct tcagcttcgg ctcagtacca gaaggacgaa ccacaacaga
    17281 gcagttatct tccagcataa acttcaaaac atcagacttc ggcagaccat tcaggccctt
    17341 actgtagtct aaagtttcaa taaccttctt accaccaatt atatcgagac ccttgtggaa
    17401 ttcaaccata atttcctgca tcttggtaaa acctgccgca ccgtcaaact cgaaggaatg
    17461 cagggtgttc aaacaatagc catactttgt ataaatctcg ttcagcttat ccagcaggct
    17521 gataccctga gtcttgtagt aagagaacat ttcacagatt agataagcag cgttaacacc
    17581 gtccttgtca cggacatagc taccggacag atagccgtaa gattcctcaa agccgaagat
    17641 atagctgtca accttaccag ccttttccaa cataccaatc tgctcaccga taaacttaaa
    17701 gccggtcagc acatcgatgg tacgaacacc atagttggat gcgatacgtt cacccaaatc
    17761 catggtgacg atggtcttga ccatcacagg gtcagcaggc atttttccat gcttaattct
    17821 ctgagaacag atgtagtcca gaagtagcag accagtctca ttaccagaca acagaacata
    17881 gtcaccatgt gcgctcttaa ctgcgatacc aacacggtcg cagtcagggt cagtggccaa
    17941 cagcagatcc gcattgtact tcttggcata ctccataccc agagacatag cttccttaat
    18001 ttcagggttc ggataaggac aagttgggaa attaccgtcc ggcagacgct gttcttccac
    18061 aacggtcaca ttgctgtatc ccatctctgc cagggtgcgg gtaacaggca ccagaccggt
    18121 accattcaga ggagaataca cgatagcaac attcttgtcc acttcttcgc caaacaggac
    18181 ggactgcccc ttcacttctt cgatgaaagc agtcagaact tccgactgaa tggatgcaat
    18241 cttagaagca tcgccatcca tctttacatc agcaaagatg tccagctttt cgatttctgc
    18301 cagaattgca gtagcagcct cggtagtaat ctgacagccg tctgcaccgt ataccttgta
    18361 gccgttatat ttagaagggt tatgagatgc cgtaatcata acaccggcat ctgccttcag
    18421 ataacgagtc gcaaaagata ctgtaggaac aggaagcagt tctggccaca agtgcacctg
    18481 aataccattt gcagcaaaga tgtctgcggc agtctttgca aacacatcag atttgatacg
    18541 ggaatcatag ccaaccacca cggaagcggc ttcatagttc ttattcagat agtctgccag
    18601 accttggctt gccttaccta cggtgtgtac gttcatgcga ttagtgcctg caccgatggt
    18661 accacgcaga ccaccagtac cgaaagacaa atttttatag aaagagtcct caatgtcctt
    18721 ctcagaatag gaacgcagtt cttctataat atctgcattg gcatgattca gccaacgctc
    18781 atattctact ttataattca tattattatc ctctcacatc cagaaactga atcgacccgt
    18841 gaatgctgat ttcagtagaa tcggccggca caaaaaggca gtccccctta tagatgtcca
    18901 acgattcatt atcaaatcga acggtaccac agccattgac acacagcaac acacggaagc
    18961 tgattgaatc cgcacgatag tgtaccgtct gtcttctctc agtgttgaca atcatacgag
    19021 agacctcgaa atgcttgcat ctgcaaagca attccgcagc tacaccagca cgatatttta
    19081 gaacacgaag cggctgtttc ggttctgaca accctttcaa gttcgctact tccagcgctt
    19141 tgtctacatg aagttctctc tttttaccat ttttgtcaac acggtcataa tcgtagagac
    19201 gataagtcag attggaattt tcctgaattt ctgcaattag tgcacccgca ccaatcgcat
    19261 gaatcgttcc cgcctcaata aagaacacat cattcttttt gaccggaacc ttctgaagat
    19321 aacgctcaac agttccctga gcaatcgctt cacggatcac agcttcttct gttattttct
    19381 gcagcccata gattaagctg gcatccagta cataccacat ctctgtcttt ccaagctgcc
    19441 cgttttcttt ttcaattgca tattcatctg tgggatgtac ctgaacagac aaatcctttt
    19501 tcgcatcaat gaatttgatc aggatcggca attctccttt ggtctgcgga tgcgttccca
    19561 gaaactctgg gtgttcttgc aacacctccg tcaaggtcat cccctgatac tctgaccaga
    19621 cacaacaaag ctcggtccat ccgggtgcgt agagcattcc aagtttccgc aagaggagac
    19681 atctcaattc cttttgcaaa ttcatcattc aggcggttcc cccccacaga taatcctttg
    19741 cggaaggctt caataaaaac ggtttctgta taacgagccc tcctccgttt tttaatactc
    19801 taactcaaat ttctgtttat ctttaacatt gatatccttg ccaagctgta cttcaatcag
    19861 cttcagctca gtatctgcca taatggtatg ccgacagcca gcactcatgg tgatcacatc
    19921 gccagcctta acaacctgct catatccatc cacgatggtc ttaccagtac cggccagaac
    19981 cacccacacc tcgtcacggt tcttgtggct gtgataattc atggagtgac tagcattcag
    20041 agtaacctta atggtcatgc tctctgcttc tacatccata accttataac taccccaaga
    20101 cttctctgcg aacatgatct gctgctcgaa cttgtcaacg aaaggcttaa tatagctgga
    20161 ctgttcctta tcggaaacca gaataccttc ccgagatgca cagatgacca catcatgcag
    20221 acccatagcc aacacaggaa catccagttc gttcaggatg tgtacgccat cgcaattctc
    20281 attcataaca cctttaccga taatggattc ctccatacct tcggtcagag tattccaagt
    20341 ccccagatcc ttccacatac cagcaaaacg ctgtacctga atcttgtcct catgctcaac
    20401 aacggcatag tcgaaggaaa tcttagtcag agtatcgtat ttgttgaaca gatcctgata
    20461 atctacaaag tcaatcagct gatgtgcacg ctccatgaca tacttcagct tgtagacaaa
    20521 cacaccaccg ttccacagtg caccctgtgc gatatattcc tttgccacag cctcagaagg
    20581 tttttccttg aaggtattta cgaaagcagt ctgctctgcg ctctcaggaa tgatgtagcc
    20641 gtacttctcg gaaggataag tcggttcaat acccatgagc accagattag cttcgccttt
    20701 gtcagcctga tcggacagag atttcagggc ctcaaaatag tcatcttcta cgtaagggtc
    20761 aaccgggcaa acaactacgg actcttctgg atcaatgccc atcacatcta ccagataagc
    20821 ggtagccaat gcgattgccg ggaaggtatc gcggcgacaa ggctctacag atacacccac
    20881 atcctcacca agttggttat ggatagcaga aacctgtgtc ttagaggttg caatcgtaac
    20941 agttgcgtcg ttatctacct tcttaatctg gcggtagaca cgctgaacca tggattcata
    21001 tccatcttca gttttgaaaa tcttaatgaa ctgcttggaa cggatgtcat tagacagcgg
    21061 ccaaaggcgc ttaccagaac cgccagacag taaaacaata ttcataatga tctctccttt
    21121 ttataagaaa gaggctgctg caatcctcaa aaatcacagc aacctcggta aattagcgtt
    21181 cagcacgcat cgggttattc aggaagtcct cgtaggacag acggatgcca tcttccaact
    21241 cagtcttata tgtccatccc agcttggttg ccttggaaac atccagcagc ttacgaggtg
    21301 taccgttcgg cttgctggta tcccacagaa tctcgccttc gtaaccaata acctttgcta
    21361 ccatctcggt cagttccttg atagacagtt ccttgccagt acctgcatta acagtctcgt
    21421 caccgctata gttattcatc aggaacacac acaggtttgc cagatcgtca acatatagga
    21481 attcgcgcag aggagaacca tcaccccagc aggtaacagt aggcagacct gcttccttag
    21541 cctcatggaa gcgacgaatc aaagcgggga gcacatgact gtgagtagga tgatagttgt
    21601 cgttaggtcc atacaggtta gtaggcataa cagagatata gtcagtaccg tactgcttgt
    21661 ttagaaactc acagtacttc aggccagaaa tctttgccag tgcataagcc tcgttggtct
    21721 tttccagctc acttgtcagc agacagcttt ccggcatcgg ctgcggtgcc atacgcggat
    21781 agatacagga agaaccgagg aactgtaact tcttacagcc attctcccat gcggagttga
    21841 tgacgttcat ttccagaatc atgttttcat acatgaagtc tgccagagcg ttctggtttg
    21901 cgataatacc gccaaccttt gcagcagcga gaaatacata atccggcttt tcctgtgcga
    21961 aaaatgcttc aacagcgtcc tgacggcaca ggtcaagctc cttatgggtg cgggtgataa
    22021 tattgttgta gtcctgacgg ttcagctcgc gcacgattgc ggaaccagcc atgccacgat
    22081 gaccggctac gtagattttt gcgtcttttt ccatcataat aatgcttgct cctttctaaa
    22141 tttagagcat ctctcattgc atgagagaaa tcaatttgtc tgcgatttcg ctcgcagcat
    22201 cataaaatgt atcaaaattt gaggtgtcta gctgtttgag gtcctcaatc acccgctcta
    22261 cgcggtggag gttttccggt gtgacggaaa ttgccagacc cagtttttgc aatttacaaa
    22321 tagttgttct atcttccaaa actgtatcgc gggcgaacag tgccatcgga cgctgtgcga
    22381 gaatacactc cgcaacagtt ccccatccag cctttgtaat aacgtaatct gccgccgcta
    22441 tatagtcctg tgtattgggg gtgctgttag aaataacggt tacgttttct cccacaagcg
    22501 gaacacctag cgttgtaatg aaatcatacg gcgcgtgact gacatcaatc ggctgtttga
    22561 accgcgcgga cataccgagt gcgacaaata caggcggttt ttcatgctgt gatttaatgc
    22621 gttgcacttc gtcgttatga atcggacgcg ccacaacgga agtcggtgtt ttttcgctgt
    22681 tttggagaaa ttccaacatt tcttcgttat ttagcgcata aagcatggtg tgctgaattt
    22741 taccgtaacg cttggcatat tctttccaaa tatgttcggg taaaaattcg cgatacagct
    22801 ctgtccaagt gaagtttcct acatacagga aaggtactcc tgccatatcg caggcatcca
    22861 ttgcccagat tggcatatcg caaagcactg cgctgacagc ttctcgcttg agccattcgg
    22921 cttctgtctt tgccaaagaa ggaacctttg ccaaaaaata ttcacagatc tcggtcagct
    22981 tacccgtgtc caccagtagg gtgccctcac ggttaatcag accgatatct gtgtttattg
    23041 gacgatagcg cacgcggtct atcagctctt tgggcagttc ctgctccaaa ttgcgctttg
    23101 caaaatccag atgatttgcg ccgcaaacca gtgtaatctt cgcttcgcat cggcagagtg
    23161 ttgccataat aatgggcaga ttccgcgcga tatgtccgaa gccgtgactg gaaagataga
    23221 aacagatatg tttattcaat gtcagcttac ttgactgcgt tcttcattgc agcttcgcgc
    23281 tttgccaatt cacggtcatg ctttgccatg atgttaacca gttcctcgta agaggtcttc
    23341 tgcggattcc aaccgagaac ggtctttgcc ttagtcgggt cgccccacag gttgtcaacg
    23401 tcagtcggac ggaaccactg tggatttaca caaacgagca tcttgccggt ttcaacatcg
    23461 tagccctttt catcaatgcc agtgccttcc caacgaatgg tcatgccatt tgctgcgaat
    23521 gccttctcgg tgaagtcacg aacagtgtgc tgctcgccgg tagcgatcac gaagtcctcg
    23581 ggagtctcat gctggagaat catccacatg cactcaacat agtcctttgc atagccccag
    23641 tcacgcaggg agtccatgtt gcccagttcc agatggtcct gcaggccttc tgcaatgcgg
    23701 ccggcagcga gggtgatctt acgggtgacg aaattctcac cgcgacgttc ggattcatgg
    23761 ttgaacagaa taccgttgac agcgaacatg ccataagctt cacgatattc cttagtgatc
    23821 cagaagccat actgctttgc aacagcataa gggctgtaag gatgaaacgg agtggtttca
    23881 cgctgaggaa cttcttcaac cttgccatac agctcagaag tagatgcctg ataaatctta
    23941 gtcttcttcg tcaggcccag aatacggcaa gcttccagaa tacgcataac gcccaaagca
    24001 tcaacatcgc cggagtactc aggaacatca aaagaaacct gaacatggct ctgtgcagcg
    24061 aggttataga tttcatcagg ctgaacaatg ctgatgatac gaatcagaga agatgagtcg
    24121 gacaggtcac cgtcatgaag ggtgatcttg cccatcaaat ggtcaatgcg agcggtattg
    24181 gaaatagaag cacggcgagt gataccgtga acttcgtagc ccttctctag caggaaatct
    24241 gccagatagg aaccgtcctg accagtaata ccagtaatca gcgctttttt catgatacat
    24301 tatcctttcc tatgtaagtg ataaggccat ataaatggct cgtaatttct aatacttttc
    24361 aagaatacac gataattttc gatatttcat agaacaatct acgcacgcat tagcacaata
    24421 ttagtttttc gtgaattgga atggcaacag tttttttgac aaattttata aggtgcagaa
    24481 cttctttccg tatgctattc cgattgtcct tgacaatgag cctcctcgga tgtgacgatc
    24541 ttcggcagga gctaacagtt aaagttagac tgcctcgcta gcgttagcac atccgagctc
    24601 attatcaagg attcgctgcg ccaatctctg gttacggtaa ccaaccggtg tctcgtagcc
    24661 aagtgtaccg tgcaaccgaa ggcggttcca ccaattgaca tagtcaaata actccaaatc
    24721 caattgttgt aaggtttcaa atgtgtattg atagacaaat tctactttca acgacttata
    24781 agttgattca gctacggcat tatcaaaagg acagccttta tgactcaatg atcgattgat
    24841 gtcaaaagtt gttgataatt catcaatagc ttggttatca aactcttttc cacgatcagt
    24901 atgaaaaatc tcaacctct
//
LOCUS       DACDSY010000023        24710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDSY010000023 DACDSY010000000
VERSION     DACDSY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.023"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            40..1938
                     /locus_tag="IWK44_000826"
     CDS             40..1938
                     /locus_tag="IWK44_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP9549372.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2237..2956)
                     /locus_tag="IWK44_000827"
     CDS             complement(2237..2956)
                     /locus_tag="IWK44_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP9549373.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            3293..3625
                     /locus_tag="IWK44_000828"
     CDS             3293..3625
                     /locus_tag="IWK44_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549374.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3612..5615
                     /locus_tag="IWK44_000829"
     CDS             3612..5615
                     /locus_tag="IWK44_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP9549375.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5634..6104
                     /locus_tag="IWK44_000830"
     CDS             5634..6104
                     /locus_tag="IWK44_000830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP9549376.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6125..6709
                     /locus_tag="IWK44_000831"
     CDS             6125..6709
                     /locus_tag="IWK44_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP9549377.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6724..7710
                     /locus_tag="IWK44_000832"
     CDS             6724..7710
                     /locus_tag="IWK44_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP9549378.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7700..8011
                     /locus_tag="IWK44_000833"
     CDS             7700..8011
                     /locus_tag="IWK44_000833"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP9549379.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8033..9814
                     /locus_tag="IWK44_000834"
     CDS             8033..9814
                     /locus_tag="IWK44_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP9549380.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9807..11183
                     /locus_tag="IWK44_000835"
     CDS             9807..11183
                     /locus_tag="IWK44_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP9549381.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11190..11825
                     /locus_tag="IWK44_000836"
     CDS             11190..11825
                     /locus_tag="IWK44_000836"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP9549382.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11855..13210
                     /locus_tag="IWK44_000837"
     CDS             11855..13210
                     /locus_tag="IWK44_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP9549383.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13402..14796)
                     /locus_tag="IWK44_000838"
     CDS             complement(13402..14796)
                     /locus_tag="IWK44_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9549384.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14888..15859
                     /locus_tag="IWK44_000839"
     CDS             14888..15859
                     /locus_tag="IWK44_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP9549385.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15856..16638
                     /locus_tag="IWK44_000840"
     CDS             15856..16638
                     /locus_tag="IWK44_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP9549386.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16807..17178)
                     /locus_tag="IWK44_000841"
     CDS             complement(16807..17178)
                     /locus_tag="IWK44_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9549387.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17312..17755
                     /locus_tag="IWK44_000842"
     CDS             17312..17755
                     /locus_tag="IWK44_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9549388.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17798..18607)
                     /gene="yidA"
                     /locus_tag="IWK44_000843"
     CDS             complement(17798..18607)
                     /gene="yidA"
                     /locus_tag="IWK44_000843"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP9549389.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18626..19993)
                     /locus_tag="IWK44_000844"
     CDS             complement(18626..19993)
                     /locus_tag="IWK44_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9549390.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(19977..20807)
                     /locus_tag="IWK44_000845"
     CDS             complement(19977..20807)
                     /locus_tag="IWK44_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP9549391.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            20970..21407
                     /locus_tag="IWK44_000846"
     CDS             20970..21407
                     /locus_tag="IWK44_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP9549392.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21483..22094
                     /locus_tag="IWK44_000847"
     CDS             21483..22094
                     /locus_tag="IWK44_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP9549393.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEEEEFSDSEKE"
     gene            22283..22789
                     /locus_tag="IWK44_000848"
     CDS             22283..22789
                     /locus_tag="IWK44_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP9549394.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            22806..23705
                     /locus_tag="IWK44_000849"
     CDS             22806..23705
                     /locus_tag="IWK44_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP9549395.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            23792..24676
                     /locus_tag="IWK44_000850"
     CDS             23792..24676
                     /locus_tag="IWK44_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9549396.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVHPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT         8028 a         4318 c         5284 g         7080 t
ORIGIN      
        1 tttataatgt tgagtatacc aatttttgga ggcgttttaa tggaccgcta tttagattta
       61 ctttcagaga agttccctac aacggaagat gtcatcacgg aaatcatcaa tctcgaagca
      121 attttgcagc ttccaaaagg aacagagtta tttttgagcg atatacatgg cgaatttcct
      181 gcttttgatc atattctgcg tatcggttca ggaaatctta aagaaaagat caaagacctt
      241 ttctctgaac aaatgacgga aacggagatc agccaattca ctatttttac ggcttatcca
      301 gaatatgtgt tgacgactga ttggtacaaa aaacaagata aatcccagct gatccatcgt
      361 ttgatcgatc ttttacggtt tacgacagtc aaatacactc gatcaaaagt tcgaaaagga
      421 ttgccgaaag aatatagcta tatcattgag gaactcttgt atatcgatga tcgtatcaac
      481 gggaaaaaag actatgtaaa aaaaatcatt gaacaacttg tcttgatgaa ggaagaagaa
      541 aaatttttaa caaaacttgc gcagacgatc caaaaatctg tcatcgatca tttgcatatc
      601 gtgggtgata tttttgatcg aggaactcaa gcagccaaag taatggatcg aatcatggat
      661 ttttcctctg tagatatcca gtgggggaat catgatattt tgtggattgg tgcgtattgc
      721 ggctctgagg catgtttatt gacgctttta cggattgccg cacgctacaa ctatttattt
      781 gaattagaga aagaatatgg attaaattta cgtccattat tttcttttgc ccatcaaacc
      841 tatcagaaga atccagtatt tacccctaaa aacaggaaag atctatctga aagagagcaa
      901 gaagaattag aaaagatcca tcaagcactt tctatcctcc aattcaaatc agaacatcag
      961 ttgttgaatc gacgacctga atttaaaatg gacgatcgta aattgttgga aaaaatcaac
     1021 tatgaggaat caacgatcgt tttagaaggt cagctctatg agttgaaagg gacttgtttt
     1081 caaacgatcc agccagatga tcctaaaaaa ttcttggaag aggaacaaaa agtgatcgat
     1141 agtttgatgt attcattcca gcactcgtta agaatgcaaa aacacatgac gtttttgatc
     1201 gaaaaagggg gaatgtatct cacgaacaat cagcatgtac tgttccatgg ctgtatacct
     1261 gtagatgaaa aggggcagct cttagcattt gaattagacc aatcttatcg cggaaaagaa
     1321 ctgctgggat tttttgaaaa acagataact gaaagcgcaa aagatttgac tgcaaatgaa
     1381 gatttggcga ccgatttgat ttggtatgcg tggagcgggc cattttcgcc tttattcggc
     1441 aagagtaaaa tggcaacttt tgagcgctat tttctcacag agcctcatac gcacgtcgaa
     1501 aaaagcaatc cctattatca cttgcgagat gaagagtggt tcagtgaaaa aattttggca
     1561 gaattcgatg caaaagaaga aggttcagca atcatcaatg gacacacgcc agtaaaagta
     1621 aaaaaaggag agtcaccgat caaagcaaac ggaaaagcat ttgttattga cggaggtcta
     1681 tccaaagctt atcaaaaaac aaccgggatt gctggctact cattactatg caattcttac
     1741 ggtttccaaa tcgtgacaca ttatccgttc ttgcctattg aacaacaatt tgcaaaaaaa
     1801 tctgatcaaa cgaatgtaaa aaaagtgatc gagcagcagc tcccacgcaa acttaatcgg
     1861 aatacgacaa aaggaatgga attacagcaa cagattactc aactccaaag gctgttggat
     1921 tatcgaaagg acgattgaga aaaatgctag ccaacattgc tcaatagact aaaaagcgac
     1981 tgcaacaaaa ggcgtgtcgc agtctccttc ggaattagct caaaaaacgg taaaatatta
     2041 gaagctattt ttctgtttat ttctctaata ctcaggtcag ggcgctgatt tcacgacctc
     2101 ttccttaaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccaacaaaca
     2161 tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac
     2221 ctaatcagaa aaacgattaa aattcattca caaagaaatc gaataaacgc tgttcagatg
     2281 aatctacacg atgagaaagt tccggatgcc attggacacc aaggattttg tgcatattat
     2341 ctttactttg gactgcttca atgagtccat cgttggaaaa ggcgatggct tttaacaaag
     2401 gagaaagatc tttgactgcc tgatgatgat aggaattcac aaagtatgaa tcagggagaa
     2461 ggtcacttaa taaactgtcc gaaacgattt tgacttcgtg agttgcaaac tgcggggcag
     2521 tgggctgttg ttcgtgtttt acggaccatt ttggatataa tgaaagatct tgataaagtg
     2581 ttccctccaa cacgacattc aatagttgaa gtcctcggca gactgcaaaa atcggttttc
     2641 tttgtttcaa tgcttccaag atcaatgcct gttcgaattg gtctcgttga atatttgttt
     2701 ccgtcagttt tggatgaggg tcttccatgt aaagttgcgg actaacatcc tgaccgcctg
     2761 caagaatgag tttgtcgatt ttctcaatta gttgaggcgc tgaatctgga gacatgactg
     2821 gcagaataag aggtaatcct tgagcatcta aaactgcatc cacaaatccc tgcggtgtat
     2881 agcttacttg atttccttgg aaagtatctg tcgctcgaat cagttgattc ccggcaatgc
     2941 cgatgattgg tttcattttt attcccccta aagtagacta attgtaacaa ggatttaaaa
     3001 aaatttaaac tatgtaagac ttgcccctat acttatctgt tcttgctgtg ttactatctg
     3061 taaagagaat tttcgtgtga aattcatgac gatagttgaa aatattgctt aatttgttat
     3121 aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct tcaaggatct agttcttcaa
     3181 ggggtgagaa ggacatttta ttgaaggggc ctgcctatac taagttatct ttagaggtac
     3241 atactttcaa tgaaaaaaga aagcatgtac ctctttttta aatcgaaagg aagtgaagca
     3301 agtgttagaa gctttggaac aaatccgaaa agcagaagaa gaaaatgaga agcgtgctcg
     3361 aaccctacgg cagcagttga agacctatgc agaagaaaaa gagcaagaat tggagataat
     3421 ccaaacgaac agtcggcaac agctgaaaca gttggtagaa gaaaaagaac aattcgaaaa
     3481 gcaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa gcagacaagg tgcaagcaac
     3541 tttgaaagac caatatcaaa agcatcagag tcaagcaatc ggtgcaatca ttgaaagggt
     3601 gaaagaaact tatggccgtc actaagatgg aaaaagtgac cctcatttca gataaaagaa
     3661 atcaggagag gattttacaa gcagtacaag gaatccaaaa tgttgaaatc cgggatctgt
     3721 atcaaaatag aacaaacaac cagtgggtag aaaaatattt tccagatacc acgattattg
     3781 ataaagaagc aaaattaagc gctttaggca atcggctgaa agacatccaa gaagctatcc
     3841 aattcataca acaccacggc gatagccgtc aaaagaaact ccatttgaaa cgtcaagaac
     3901 tttccttaca agacttggaa gcaaattatt ccgaagaaaa tttcatgaat aaattagaag
     3961 aagtccttga tttaaaagaa aagtgggaac agctaactga aaaacgtgat cgtttagaag
     4021 aagaggaaga atggcttctt cattggcaac agctggatat tgctccaagt atgtatgcaa
     4081 gtaagacgac catttttgag atggggactg tgagtgcagc aaattttgaa agtttcaagg
     4141 aagaactaag taagcttgat gacgtttata tggaagaaat agattatacg ccaaaacaag
     4201 tttatctttc gtatgttgct ttaaaaaaat cagttgctgc tatagaagaa attgctggga
     4261 aatacgggtt caataaagaa acgtatcctt atgaacaaaa tcctaaagaa aaattgcaag
     4321 aaaccaaaca gcaattatcc attgttcacg agcagcaaaa aaagataacg acagcgttag
     4381 gtcagtgcag cggctatatc caagaatttg aatgggtaga agaagtcaca cttgccgttg
     4441 cagaacggga aaaagtaaaa gaacggttta tccatgccac ttatctgatc gttttgcaag
     4501 gttgggtaga tgcagaagaa aaacagaatt tgatgcatgt gttaaacgaa actgtatcag
     4561 aagaagatat atacgttagt ttcgaaacac caaactcaga agagattcag caggaagtcc
     4621 ctactaaatt gaaaaaccat ccgcttatcg aaccttttga attgctgact gaaatgtaca
     4681 gtctgccaaa atatgaagaa gtagatccaa cgccttggat gacaccattt tatcttgtct
     4741 tcttcgggat gatggtcgca gatgttggtt acggattgct gatgctcatt gggacaatac
     4801 tagctcaaaa actatttgtt ttaccccgcg ggatgaaacg tttcgtaaaa ttctttgaga
     4861 ttctctcggt accatcgatc atctggggat tgatctacag ttcatttttc ggacaggcat
     4921 taccaaaaga actatttggt atccgcttgc ctttcccaat cctatctacg acggatgacg
     4981 tcaatacgat tttgatttta tccgtgattt tcggtctgat acagatcttg gttggattat
     5041 tcgtggcagc aaaggaaaat atcaggagga aagattactt gagtgctgtc agtgatggtt
     5101 ttgcctggca agggatcctg attgggatcg ttattgcatt gattggtgtc atgttgataa
     5161 aaaatacagc atttgtttat cttggcgcgg ggctatcgat cattgctgct ttatgtatct
     5221 taatcgtgcc gattatccaa tcgccgtcta aagtcaaggg tgctgccaaa ggagcgtata
     5281 atctttacgg attgactggt tatatcggtg atttagtcag ttatacacgt ttgatggcac
     5341 taggtatttc aggcggaagt attggcgcgg cattcaatat gctagttgct ttcatgccgc
     5401 cagccgctcg attcagtgta gggatccttt tgatcatcgc tttacacgca ctgaatatgt
     5461 ttttgacact gttaagtgcc tatgtccatg gtgcacgatt gcagtatgtc gaattttttg
     5521 gaaaatttta tacaggaggc ggacgtgcct ttgacccgct gaaaacggcg gaaaaatacg
     5581 tcaacatcaa tcataagaaa aaaaacaata aataattgga ggatttttat acaatgatgg
     5641 attacttaat tagtcaaaat ggtggaatgg tatttgcagt attagcgatg gcaacagcga
     5701 cgattttttc agggattggt tcagctaaag gtgttggaat gacaggtgag gcagcagcag
     5761 cgttaacaac aagtcaaccc gaaaaattcg gacaggcttt gattctgcaa ttgcttccag
     5821 gtacgcaagg gttgtatgga ttcgttatcg cctttttgat ttttatcaac ttaggaaatg
     5881 atatgtcagt tgtacaagga ttgaactttc taggcgcttc tctgccgatt gcttttacag
     5941 gtttgttttc aggtatcgcc caagggaaag ttgcagcagc aggtatccaa attttagcga
     6001 aaaaacctga acatgctaca aaaggaatca tttttgctgc gatggttgaa acatatgcga
     6061 ttttaggttt cgttatttcc ttcctattag tattgaatgc ctaagcataa aggaggaaaa
     6121 gcagatgaac gccattgaaa aaatcatctc tcagatgaat gaagcagcag agcaagaaag
     6181 agcggcgtta gagcaagaag agcgaatgaa gatcgatcaa aactttgaac aaaaacgtac
     6241 gcaaatcgaa acagaacatc aaaaacaaaa agaaaaacaa atcgaattac ttgaaaaaaa
     6301 ataccgtcag cttaggaatc gccaacaagt agaagtacga caagaaaatt tgaatgcgaa
     6361 gcaggaattt ttacgacgat tgtttgcaga tgcaatagct gaaatggaga attgggatga
     6421 atctgaacaa atccagttta tcaaaaattc attgtattca ttacctttga caggcaaagt
     6481 aacattcatt gctggagaaa aatcagcagc ttatctttct cagacattgc tggatgaatg
     6541 gaacaatgaa cttcccttta tgatggtttt aagtgatgaa actgttgctg atcaagcggg
     6601 ttttctgata aacgatcaag gagttcagta taattttctt ttcagcagcc ttgtacaaga
     6661 tatccaagga acgatgagtt ttgagattgc caatcaatta ttcgaataag gagggatcag
     6721 tgtatggaat accacgaact aaatcccttg attcgcggaa gagagcttga acttttgtcc
     6781 aatgagacat ttgaacggat gattcaaaca aattctgtcg aagcacttgg tgaaatattg
     6841 aaatccacga tttacagtcc atatatttat gacggatatg aatatgactt cgaacagaat
     6901 cttttaaagg agcagagccg tctatttcgc tggttaaagg aatcagcacc ggagcctgaa
     6961 atcgtttgga tctatacgat gcgctatact ttccataatc tgaaggtttt gacaaaagcc
     7021 gagatgacaa atcaaaatct ggaccatttg tatatcgacg atggatttta ttcattagaa
     7081 accttgaagg atgccattca tacgcaagtt tcaaatgaac tccctgaaag cattatggac
     7141 agtatacgag aagtacgtga ttattttgaa gagtctacca ttcttcaggg aattgacgtg
     7201 atttatgatc gacagttttt gacagagcag cgacggcttg ccgataagct aggatatcaa
     7261 gaattgctga cagaagtcat tgcttttatt gacttgacca atatcacaac gatggctcga
     7321 ggaatattgc agaaaagaac atatgggttc atgacgacgg tactttccag ctcgggaagt
     7381 atcgaaaaag aagtattttt gaactttgta gataaagata tggaagatta cacgaatttt
     7441 ctattagaga cagattatgc tgattttctg cgccctgtca tccgtgaggg aaatcttgat
     7501 ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt cattgtatca agaagcacaa
     7561 acccaggctt tcggaccact gccactacta gcctttttga acgcaaaaga ggtagaaagc
     7621 aaaaaccttc gtttactggt tatcggcaaa agaagccatt tcgaaccaga aacaatcaga
     7681 aagcgggtga gacaagtcta tgacgcataa gatcggtgta gttggcgaca aagattctgt
     7741 tttgccattc aaattgtttg gctttgatgt tcgttatggt acaaccaagc aaaccgttcg
     7801 tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg atttatgtca cagagcaatg
     7861 tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa caaatgatac ctgctatcgt
     7921 attgattccc aatcatcaag gatcattagg gatagggctg gaagaaattc aaaaaaacgt
     7981 agaaaaagca gtaggtcaaa atatcttata gtatgaaaag gagatagaaa ttttgcaaat
     8041 tggaaaaatc ataaaagtct ccggacctct tgtaatggct gagaacatgt ctgatgccag
     8101 tatccaagac atgtgtctag tgggagatct aggagttatc ggagaaatta tcgagatgcg
     8161 tggcgatgtg gcgtctattc aagtatatga agagacttct ggaattggtc caggcgaacc
     8221 ggttcgctct actggtgaag ctttatctgt tgaattagga ccagggatca tttcacaaat
     8281 gtttgacgga atccaacggc cattagatac ctttatggaa atcacacaaa gtaatttctt
     8341 agggcgcgga gttcaattgc ccgcattgga ccatgagaaa aaatggtggt tcgaaccaac
     8401 agtagaagcc ggagaaacgg tgagtgctgg agatgttatc gggatcgtag aagaaacaaa
     8461 agttataaaa cataaaatca tggtgccaaa tgggataaaa ggaacgatca aaacaatcga
     8521 aaaaggttca ttcactattg atgaaacagt atgtgtcatt gaaaccgaaa atggtgacaa
     8581 aaatttgacg atgcttcaaa aatggccagt aagacgtagt cggccaatca aagaaaaatt
     8641 aaacccggat gcacccatga tcactggaca gcgagtgatt gatacattct tcccagtgac
     8701 aaaaggagga gcagcagctg tacctggtcc ttttggtgca gggaaaacag tcgttcagca
     8761 tcaaattgca aaatgggccg atgtggacat ggttgtctat gtgggatgtg gcgagcgtgg
     8821 aaatgagatg acggatgttt taaatgaatt ccctgaattg attgacccta atacaggaga
     8881 atcgttgatg gaacgtacgg tattgattgc taatacatca aacatgccag tagcagctcg
     8941 agaagcttct atctatacgg gaatcacaat tgcggaatat ttccgtgaca tgggttatga
     9001 cgttgctatc atggcagact ccacttcacg atgggcagaa gcacttcgtg agatgagcgg
     9061 acgtttggaa gaaatgcctg gtgatgaagg ttatccagct tatcttggtt cacggcttgc
     9121 ggaatactat gaacgatctg gaagagtcat tgcattagga tcagaaggtc gcgaaggaag
     9181 tataaccgca atcagtgctg tatccccgtc tggaggggat atttcggaac cagtcacaca
     9241 aaatacattg cgggtcgtaa aagtattctg gggtcttgat tccagtttag cccaaaaaag
     9301 acattttcct tcgatcgatt ggatacaaag ctattctctt tattcaacag aagtagggaa
     9361 gtatatggat catatccttc aagctgactg ggcatccatg gtcaccgagg gcatgcgtat
     9421 tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg gtagggattg attccctttc
     9481 agataatgac cgtctgacat tagaagtggc aaaatccatt cgtgaagatt atttgcagca
     9541 aaatgctttt gatgatgtcg atacattcac ttcaagagag aaacaattca aaatgttgaa
     9601 gatgatcttg acctttggtg aagaaggacg ccgtgcgttg tcgttaggtg cttatttaag
     9661 tgagatcatg gacggtaccg tatcgattcg tgaaaggatc ggacgaagca aatatatccc
     9721 ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa atcaagcaaa cgatgcaaac
     9781 gatcgtttca gaaggaggca tgagcaatga ttaaagaata tcgtacgatc ggtgaagtcg
     9841 tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa atatgaagaa ttaatcgaag
     9901 tccgtatgca aaatggcgag attcgtcgtg gacaagtatt ggaagtccaa gaagataaag
     9961 caatggtcca aatattcgaa ggaacaagtg gtatcaattt acgtgactct tctgtacgat
    10021 ttttaggcca tcctttagaa ctaggtgtat cggaagacat gatcggtcga gtattcgatg
    10081 gtttgggacg accgaaagat aacggaccag agatcctgcc tgaaaagcat ttggatatta
    10141 acggagaagt aatcaatcca gtagctcggg attatccaga tgagtttatc caaacaggta
    10201 tctctgcaat cgatcatttg aatacattag ttcgtgggca aaaattacca gtattttctg
    10261 gctctggtct tcctcataaa gaattagctg ctcagatcgc gcgacaagca acggtactcg
    10321 attcatcaga tgatttcgcc gtagtctttg ctgctattgg aatcactttt gaagaagctg
    10381 aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga ccgttctgtc atgtttatga
    10441 atttagcaaa cgaccctgca atcgaacgta tcgctactcc tcgtatggct ttgactgctg
    10501 cagaatatct tgcttatgaa aagggaatgc atgttttggt tatcatgaca gacatgacca
    10561 actatgcaga ggcattgcga gagatttctg cagcacggcg agaagttcca ggaagaagag
    10621 gatatcctgg gtatctttac acgaacttag ctactttatt cgaacgagca ggaagaatcc
    10681 gcggactaaa aggatcggtt actcaaattc cgattctaac aatgccagaa gacgataaaa
    10741 ctcatccaat ccctgacttg actggttata ttacagaagg acagatcatc ttgactagag
    10801 agctttacaa gagtgggatt cagccaccaa ttgatgtact cccgtctctt tcgcggttaa
    10861 aagataaggg aactggaccc ggaaagacaa gagaagacca tgcagctacg atgaatcagc
    10921 tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt agccgtagtt ttaggtgaat
    10981 ctgctttatc tgatgtagac aagatctatg cgaagtttgc tgagcggttt gaaaaagaat
    11041 atgtcaatca aggattctac acgaaccgtt cgattactga aacacttgat ctagggtggg
    11101 aactgctatc gatgttgcca agaaccgaat taaaacgaat caaagacgac atgctggaca
    11161 aatatctgcc agaagggaag tgagttctga tggcacgatt gaatgtgaat ccgacccgca
    11221 tggaattgac tcgattgaaa aaacagttga caactgccac aagagggcat aaattattaa
    11281 aagataagca agatgaactg atgcgtcaat tcatcctgtt gattcgaaag aacaaccagc
    11341 tgcgtcaaga aatggaagag tctatgacag aagcaatgtc tgattttgta ttggcaaatg
    11401 cttcgatcac ggaacctttc atcgaagaat tatttgtttt gccggcggaa aacgttgatt
    11461 tgtctatcgt agacaaaaat atcatgagtg taaaagtacc tgtgatgaat tttgaatatg
    11521 atgaacagct gacagacaca cctttggaat acgggtattt gaattccagt gccgaactgg
    11581 atcggtcaat cgatcggttt actaccctgc ttccaaaact tttggaactg acagaaatcg
    11641 aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac acgtcggcga gtcaatgcat
    11701 tagaatatat gacgatccct caattagaag aaacgatcta ttatattcgt atgaagctgg
    11761 aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt aaaaaatatg ggtaccactg
    11821 actaagtggt atcttttttt aaaagaggaa aacgatgatt aaacaaagca aacaaataaa
    11881 gaaacgtttg ccggctgttc agcttctcgc attaggattt tttctcctga ttttagttgg
    11941 cggcggccta cttaccctac cattttttag taatagtggg catgggacaa attttatcga
    12001 tgcgcttttc actgcaactt ctgctgtctg tgtaacggga ttgacaacat tgaatacagc
    12061 agagcattgg aacagtgctg gtcaattttt gatcatgctt ttgattgaga tcggcggtct
    12121 tggttttatg atggttccga ttctatgctt tgcattgata aagaagaaag tcagtctcag
    12181 cacacgtatt atattaaaag aagcgctgaa tctagaagaa gtatcaggtg tgatgaagct
    12241 catgctttac atcctaaaat tggcctttgt tatccaattg attggtacgg ttgctttatc
    12301 ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc tggtacagtt tgttccatgc
    12361 aatctctagt ttttgtaatg cagggtttga tttgttagga gacagtttag ccaatcacca
    12421 gcaaaatgtt tatttgatca tggtggtatc tgctttgatc atttctggtg ggttaggctt
    12481 catcgtatgg cgtgatttac tggaatatca tcagaaaaag aagatgacga ttcattcaaa
    12541 aatcgcatta accgttacag gtaccctttt attaggcgga ttcgttgtgt tctttttcac
    12601 agagcgcaat ggggcaacgc tagtggaagg gaatatgttt caacgattgg caaacacctt
    12661 ttttatgagt gtgacaccta gaacggctgg atattattca gttgattacc tccatatgag
    12721 ccatgcaggt ctgattttaa cgatgttttt gatgtacatc ggcggaacat ctgggtcgac
    12781 ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg atccagatgc gttcgatgtt
    12841 gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc cgacaaggcg cagtgttgcg
    12901 ggcacttact ttattttttg ttacgttgac gttgtgtgta gtcgccatca tgattttatc
    12961 ggtgactgaa acgataccta aaacttctgg catcgaatat attgcttttg aggtcttctc
    13021 tgcgtttggt acagtaggtc ttacgatggg attgacgcca gacttgactg caatagggaa
    13081 attgatcatt atagcgctta tgtacattgg acgagttggt gtattgactg tggtcttctc
    13141 tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa tatccagaag aaagtgtcat
    13201 gattggataa tttcatcttc tgacagaaac ctagtgattt agtaaaataa gaaaataaaa
    13261 taatttttct tgcttcttgg atactttaag tgcaaaatgt tcgtttattt tttgaggcac
    13321 tacccgttgt tgaacctcgg gtagtgcctc ttttttattg aaaggaatca ataagatttg
    13381 attcctttat ttatcaatag gttataattc atattttttt attttttctt ttacgatact
    13441 tagtccagaa gctaaaattt caaggttatt agaggatagg gaaattcgaa cgaatgcatc
    13501 tggttgcgca cgtgtagtaa agcgtctgga atgataaagt cggataccgt tagcaagaaa
    13561 gtcattctca attatcttag ggtcaatctc aggatcaact ggaagaattc gatagtaagg
    13621 gacacgattc gttggttttt ctactttgaa aagagaatca aaaagctgat tggctctttt
    13681 tgtaagactt aatttagttt ctaagatcct atttgcttca tttgaatgga ggatttgagt
    13741 gatgacttcc acatctaatc cagaagtttt cacatttata ttaaaaattg cttgttctat
    13801 cttgctttgg acatgtactg gaactacaag ataagcaact cgcaatccag aacaaataaa
    13861 ttttgtcatt ccagctaagt agattgtttg gttaggtaat aaattttgga aaggaggaat
    13921 gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata tcatcttcaa taacccatag
    13981 accttctttt tcaatcactt cggctaattg ctctcgtctt ttgtgagaga tttgaaaccc
    14041 tataggatta ttacaagaag gcataagaaa gacaccatga atcttctttt ttctacattc
    14101 ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa tcaattggaa cgatctctaa
    14161 atgatataac tgagccaatt caatgaaatt agcgtatgta tatcggtctg ttgctattct
    14221 atcacctgga gagaaaagtg cagaaagtgt gatagccaat ccattttgaa caccggaaac
    14281 aatagataga gattctaagg acggaatgac tccttgagtt tttaaccaat ttgctgcagt
    14341 agtcaattga aaatttgtgc ctttgggatc ttggtaatta agcaaaccaa caagatttgg
    14401 gttattacta gttgattgaa taaacgggac aacgaattta ttacattctt caaaagaact
    14461 aaccagacca aaatcaatga tatgttcggt tacttcattg gtagagatgg tgatggactc
    14521 aaagacattg ggagaaataa atgtgccact tccaatattc gtatacagca aacctttctc
    14581 aattccatat ttataagcct tgccaacggt agtaaaattt aaatctaaat aatcagctag
    14641 ctctctttgg gagggtaact gagtgttttt ctgtaattta ttctttaaaa tatcttgttc
    14701 taattgttga atcaacgatc gataataagg acgcttcaat ttttgtttgt ctggtcgcca
    14761 agttaacggg taattgtcat aagaattaat aggcattttc tccactcctt aaaattgtat
    14821 accatacaat tttaccattg attgtattga ttttagaagt taatcttttg tgaggaggga
    14881 attgtctatg aaaattatta attttgagga agtagtagct tcttttagtt ttgaagaagc
    14941 gattcaagta atgaaaaaat gtttttttga ttatgaaagt ggtaaaatca gccaaaatcc
    15001 tcgattggtg gaaattttac ctgacggaaa aggcaaaaat atgtttgcga tgatgcctgc
    15061 ttatttgggg gagaacagat attttggcac aaaagtcatt accgcttttc cggataatca
    15121 tcagtatcat ttaccttctc atttaggaga gattttcttg tttgatgctc aaaacggact
    15181 acccaaagca ttgataaacg caaatgcagt cacctggatt cgaacagctg ctgtatctgc
    15241 gctggcaaca gattacttag caaatccgca tgcagaaaca cttgctttaa ttggagctgg
    15301 tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt cgtcccatca ctacagtgtt
    15361 tgtttacgat attttagaag aaagacgaaa tacttttatc gctaaagcta aaaaacagta
    15421 tcctaaaata acgtttataa atgcaatatc agtagaagat gcagtacgtc cagcggaaat
    15481 catttgtacg ttgacctcta gtaaagaggc atttcttcaa aaagagtggg taagggaaga
    15541 cgcacatatc aatgctgtgg gcacatttac tcctgataca agagagataa gtacatcgtt
    15601 gatggaaact tctcaaatct atgtagatga ttactcagct gccttaacag aaagcgggga
    15661 cctgcttata gccatggcag aaggagattt agaggaatct tgtattgttg gtacattagg
    15721 cgaattagtt tctgagaaaa aaacatttta taaaggagaa tcgaggccga cgattttcga
    15781 cgcagttggt ctagctattg aagatctttg ttgtgcagaa tatatttata acaaattaga
    15841 gaaaggggct ttaaaatgaa cttagattat tacgtagttg atgcatttgc agatgaggtg
    15901 ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat ggctgccaga aggtacgatg
    15961 caaaaaattg ccattgaaaa caatttatct gaaacagcat ttacagtgaa gaaaaaccaa
    16021 gaatttgagc ttagatggtt tacgccagat cgagaaatcg atctttgtgg tcacgcgaca
    16081 ttagcaacag catttgtttt atttaactat tacaaaatac ctgatgaaac gattaaattt
    16141 tctactcaaa gcggtaatct atttgtgaca agaattaaag attattatta catggatttt
    16201 cctagtatta tgccgaaaaa agtgccgata ttgtctgaat atgaagaagc gattggggct
    16261 aagattaaag aagcctactt agcacgtgat ttatttttcg tattagaaga tgaaaaaacg
    16321 gtggctaaat tgcagccaga ttttacagct atcaaaaatt ttgaattagg aataggagtc
    16381 atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta gaaccttctt tccaaaatta
    16441 acaatcaatg aagatccagt atgtggatca gcacattcta atcttattcc ttattgggca
    16501 gaaagaatga ataaaacaaa attgtcagct tatcaaatgt catcaagggg cggggatcta
    16561 gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa aagcagtttt gttttctaaa
    16621 ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa taaattggtg gagagtggca
    16681 accttatagc tacaatggtt gttaaaaaaa gacgcaaact ttttcaattt aatgaaaagt
    16741 ttgcgtctca ttttaattat tggtgtttgt tttttattca atcaagagta aaatgcccca
    16801 tactaactat cctcggatga cttcgatttt ataaccatca gggtcaatga caaagtagta
    16861 agaaggcgca gtgccaggta atccttttaa gtcagtaatc tcaagaccgg cagctttatg
    16921 ttgctcgtgt aatttttcta gatcatcagc tgctatagcg atatgaccat agccgtctcc
    16981 tagatcatac gcaccatgat catagttgta agtcaactct aattcatagt catccccagg
    17041 aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa tcacggcggc ggctttcttc
    17101 aaaaccaaat gccttttgat aaaattcgat tgatgcttca agatctttca ctcgaacaca
    17161 tgtgtgagcc attttcatag attcatctct ccttttcctt ttactcaatc ataacaaaaa
    17221 gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa tttctcatgt aaaatggata
    17281 gaaaataaaa ctgttaaggt ggaaactatt catggataaa ccaatttttg gtgaaaaaaa
    17341 gcaatttgct gattatcaaa cccgttatgc agcatatatt gtcatagaaa gagatcagca
    17401 gattgcagta atcgaggctc caaatggtgc tttttttctt cctggcgggg aaattgaagg
    17461 agaagagacg aaagaagaag cgattgtccg tgagctgata gaagaaatgg gaattgtggc
    17521 agaaatttct tcttatctgg gagaagctga tgagtatttt tactcgaatt atcgtgcaac
    17581 ttattattat aatccaggat atttctttgt tgctgattct tggaagcgta taggagaacc
    17641 aactgaaaag accaataaaa tttggtgggt gagccctcaa gaagcgcttg tgaaattgaa
    17701 acgtggtagc caccgatggg cagtcgagag atggatcgaa aaaaataaaa actgaaagaa
    17761 agcgagtaga tcagctttct ctcagttttt ttatttatta cagtacatat tttttgattg
    17821 cttcagctac accgtgctcg tcgtttgatg aagtgatgac atttgctgct tctttgacaa
    17881 gagaaacggc atttcccatt gctactccta gaccagcata ttcaatcatg gataagtcgt
    17941 tctcgttatc tccgatagcc atgatttcat cttgatcaat accaaggtgc tgtgcgagat
    18001 tcgcaactgc cgcaccttta cttgcttctt tatttagtac ttcgaagtaa tagggcgcac
    18061 ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa acgcgcaatt gctgcatcta
    18121 aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt catgtctggt gtcatttctt
    18181 caggtgttcg atattttagc ggcatcttga ctaaacttgc ttcgtggatc gtataagcgc
    18241 tgatatctcg attaggcgtg tagatcgctt tgtcatcgat cgaatggaga tgactgccga
    18301 cacggcgtgc catcacttcg atctctagga aatcttcatg tgtcagtccg tgacgggcga
    18361 tgattttacc tgatttcgtt tgttgtacga gtgccccgtt ataagtaata acgtaatcat
    18421 tatcttgaaa aaggttcaac tcgtttaact gttcctggac acctggtaag ggacggccag
    18481 tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc aatcgcttct tttacttctt
    18541 ttgttacttc tcttttctca tttaataaag taccatcgat gtcgatagcg actagtttga
    18601 tagacataat ttcctccaat atattttaat ttatttcaat caatgcgcca ttacggatat
    18661 ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata atgatcacgc aaggacggat
    18721 caaccatttc ttttggaaag aaaaatcgct catctccttg ttcttgtccc gctaatgcag
    18781 caacaagctg actaacctgt gataattcta tcaaagttcc gtcatttcgc aaaatctcga
    18841 tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc ataaggtaga tcgtagctag
    18901 aattcacagc agtgtaataa acaggattat atccgacctt ttctactaac aaagtgagtt
    18961 cttggactaa ttttgaatcc cggttgccag aaaaagtagc agatttcagt ggtttacgat
    19021 tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc agattcagac cattgagtaa
    19081 agtaagtgcc caagacccca tcatcaagtt tgaggtattc ttctaaagta aagcttccgt
    19141 caagaaatgg cagaagtaat tgagaatgga gttcaaaact gctggcatct tcttgataca
    19201 actcgtgtgc gcgatgaagc agatgctcca aaattacttc cataccacgg gaaacaggat
    19261 ggaagtacac ttgcacatac atttgatatc ggctaacgat ataatcttcc actgcatgca
    19321 tgccactcat ggaaaaagcg atcccgcctt cataaggacg aatcacacgt aatattcggg
    19381 taagatcaaa tgttccatat tctgttccag tgaaataagc atcccgaagc aaatagtcca
    19441 ttcgatctgc atcgatctga ctagaaatca tttggacaac ttgtgggttg gggtaagttt
    19501 tttggatcac actagctacc ttttcaggaa aaccttcttc tactcggtta agaatctgat
    19561 acacttctgt ttctggagaa gtgatgatct ctacagtgat ggcttcatga ttcgtatgga
    19621 agatatgttc aaatgtatga gagtaaggac catgaccgac atcatgcaac agtgccgcac
    19681 ataaagtcac cagccgttcc tgatcatccc agcctccgtt gcctattttt tcttttgaaa
    19741 aatttcgaga aaagatatcg cagattcgtc ttgaaatctc ataaacgcct aatgaatgag
    19801 taaaacggct atgttctgct ccgtgaaaag tgaaagaaga agtgcctaat tgtttaatgc
    19861 gtcgtagacg ttggacttct ttcgaattga tcaaatctaa gattacttga tgctggacat
    19921 ggatatagtt atggactgga tcacgaaaaa ctttttccat aggcagcatt tgatatttat
    19981 aaggggttgt cataatgtag ctcctttatg ttattacgtt gttccattct ctggatttgg
    20041 ttgttccatt gctccagctg gatttctttt tttagccagt cggtagaatc ctgtaaatca
    20101 cttgcagaat acagcttttc aaaagctttt gcaaatgctt ctttgacttc ttttacaaaa
    20161 aggggttggt caagcagttc ttcaagtgtt gccattgtag caggatccac tgcaggatag
    20221 ccatttgtac caaagtcttc ttttaaagca gattgataaa atcgcttcat gacttgcccg
    20281 cgtttttcct gattaccgtt gacacttaag tacatcataa cagaaatccc atttttgatc
    20341 cgacgctgag cgataccggc aaattttttg ccatggatgc ttaaatcata agttccagga
    20401 caataagaat gggtgatttc gaatgcttgt atcggtgcct ttggaaaagc gtatttagtc
    20461 aataggagca tttgctcgta agcgtcatct atggttattt tttttgtgtc actttggggg
    20521 aaaatcaaag aaatattcaa aattccttca tctgatacga tccctaatcc acctgaattt
    20581 ctgatcactg gagcgtaacc tgcatctttc agtgtttgga tcccgtcact tagaaaagga
    20641 gttcgaacat ccttcatacc taagatgact gtgcagtcat attgccagaa atgtaaaaag
    20701 atttgctggt gttctttaga atattccgtt aatatatctg ttaatgcaaa aggggaaaag
    20761 tcgttttttt gataaatgtg ctgatctagt cgaatcgcag ggttcattca accggctcct
    20821 ttgtataaat tcatattttc tacaatatta tactctaaaa tcaaagagtt gttttggaaa
    20881 aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac aaaagaaact aggaacaatg
    20941 tagctatgag aagtaaagga gaatgaataa tggaattatc acaaggaaca ccgatttcaa
    21001 tcaaattgcg tacaaaagta aaacagcaag gggaagtgca agatttttat tttgatttga
    21061 aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg ttataaagag attcaggagg
    21121 agacgggcga tgagattcct gtaacgatca aattagttcc agatggtcat gtacagctga
    21181 tccgtgcagg agaaatgcgg atgcgtttga aatttggcta taaagaacgg ttagaaacat
    21241 cctatcgaac accatacggt atgatccaga ttgctacttt tacgaaagaa ctgcatgtta
    21301 gtttgaaaga ccgtcccaca ggagggaagg ttcgaatcga ttatgatctg ttcatgggac
    21361 cggaaaaaat cggcgaatat tatttgacct tggattttac agcgtaaata taaaagaaaa
    21421 gtttgatttt ttgtgtctga ttttgtatga taaggagtag actgtgaaag gacgtgtcct
    21481 gattggaaat tagtgtattt gaaggtgcaa acaaaagcga attatcaatg atcgaagtag
    21541 cacacgcgat cttagaacaa cgtggcgatg tcatggattt ttctgattta gctaaccaga
    21601 tccaaaacta tcttgggaaa tcagacagtg aaattcgtga ttcactcgca caattttata
    21661 ctgacttgaa tattgacggc agctttatct cattagggga taatcgttgg ggcttgcgtt
    21721 catggtatgc tatcgattcg atcgatgaag aagtaaacca tggaatggac gaagaggatg
    21781 aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc ctttatcaat gatgatgaag
    21841 acgcgatcga ttacaatgac gatgacccag aagatacaga tctgacagaa gaagatgacg
    21901 atgatctgtt tgatgacgat gatgatgaag atgaagaaat cgctgcttat aattctgact
    21961 tgcaagaaat cggagcagat gacccgacag atgatgaaga agacttgcca ggaaatatcg
    22021 aagaagattt aagtatcata gacgaagacg atgatgatga agaggaagaa ttttctgact
    22081 ctgaaaaaga ataaacatta ttttcttacg agaggttgtg acagagacga tcagcctatc
    22141 agaataagga ggtgtgacac gatttttgtc acaccttctt ttttgtatat aaaaatccca
    22201 agtaaataca taaaaaataa aaaaattgaa acttttagcg caccttgtac gtcatattaa
    22261 gtgaggcaga aaggagtgga gaatgatttt tactggatat aagaaaaaac ggcagatcga
    22321 aaaataccgt cacctgctga atggactcat cgaaactgat tatcaaaaaa tgttccgtat
    22381 cacgatgaat tacgtacatg ataaagaaga ggccttagat gtgatgcaag atagttttca
    22441 taaagccctg aactattttg aaaaagaaaa agagatcgag cattttagtg catggtttta
    22501 tcggatattg atccgcacgg cattagatag ttggcgcaga caaaaaagga cgcctgtgga
    22561 cttatttgaa attcaagata tcccccaaca gttaccttta gaaacttccg ttgcagagct
    22621 ccatatgatt ttagataaaa tagacagtcc ggaaaaagag attctgatcc tctacttttt
    22681 tgaaggattt cgtttgaaag aaattgcgac tatcttggat atgaatgaaa acacagttaa
    22741 aacgaaaatg taccgctcat tgaatgcttt acgtcaaatc ctagaatgaa ggagagaaga
    22801 gtgccatgta ttcccaaaaa gaaaatttaa aaaaactaaa gaaaacttat aaagaacaag
    22861 caattcccaa agggcttaaa gaggagatcc gccagcgttt tattatcgaa gagcaatcat
    22921 ttataaaagc aaagcggaga aagagacgtc tccaagggtt cggagtatct ttggctatcg
    22981 taatgattac aacaagtagc cttcttttta atagtcaagt tcgaagcttt gcagaagatt
    23041 tacccatctt aggatcagtc attgaattga tcttaggcga acgctttact gatcgatctg
    23101 aaaaaatcga tattcaggta ccgaagatca atacacaaaa tgagaaagaa aataaaacaa
    23161 ttcatggatt aaatcaaaaa tactttcgcg aaggacaagc agattttgaa aaagctgaaa
    23221 aagaatatgg aaattttgaa acggatcatt accaagtttt gggagattat cagaaaatcg
    23281 tagacgataa ccggtttctc gtgattgaaa ggcagatcac ccaaacagca gcagatagcc
    23341 atgttgaaaa aagatacgac acaatcgaca agaaaaacag tgtccagctc tcacttccac
    23401 ttttattcaa agatgacacc tatctatctg ttttgacaaa agaagtcaaa cggcagatgg
    23461 ccgaacaagt caaggaagat ccatctaagt actattggac tgagcaagat attcaagaag
    23521 ggacgataga aaaaccaaca cttgttacgc ctacacgaag tttttatttg aacaaagagc
    23581 atcaattagt tttgactttt tctcaatacg aaattgctcc tggttatatg ggtacaccag
    23641 aatttgtcat tcctaagtcc gtgacaaaaa cgattttggc atcggaagat tatttggatc
    23701 attagacgag aagtagttgc gactagcagt ttcaattttc aagtaagtac aagtaaataa
    23761 taagaagaga aaaagagaaa gaagagatat agtgaaaaga aattatttag ttatcagtat
    23821 tattgcttta tccgtgcttt tgtttttcga aattacttcg gtggcctcct tgatcaccga
    23881 agtaaaagga caaacacact cttcggagca ttcgtcacaa tctttcaaac aatcagaaca
    23941 atcaaccatt aaaagccaat caactgaaac agctaacgtc aaagaagcca ttcaagaatc
    24001 agcggaaaag gtacaaagtc agtcatcaca atcaacacaa gacagtacaa aaacgagccg
    24061 agatccggct gtcaaaagcg tcgcaatcag ctttgatgat ggaccaggag cgaccacaac
    24121 accgcagctt ctgcgaatct taaaagaaaa aaacgtgcat gccacttttt tcgtcttagg
    24181 agaaaatacg gcgcagcatc ctgaaatcgt gaagcagaca gctgaagcag ggcacgagat
    24241 tggcaatcac acatacgatc atcagaatct ggccactctt tctgcccagt cgatgacgga
    24301 ggaagtgacg aaagcagaca ctgagatcaa aaaagtcact ggcaaaaccc ccacttttgt
    24361 tcatccgccc tatggttctg tcacgaatgt aggagcaacg atcatccagc gaccgatcat
    24421 cgaatggtct gttgattcag aagattggaa gacaagaaat cctgatttga ttttgcagaa
    24481 gatccaagca acagtatacg atggggcaat tattttattc catgatatct atcctgaaac
    24541 gattcgtgcc gttcctcaag tgatcgatta tctgaaagag caagggtatc gcatcacgac
    24601 agttggggat ttattagggc atccaacggc tgtcgagaac tattatggca gaaacgatca
    24661 tcggccagtt cagtaagtga gacaagtatc tttaataaat aacacatttt
//
LOCUS       DACDSY010000024        24557 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDSY010000024 DACDSY010000000
VERSION     DACDSY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.024"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            99..476
                     /locus_tag="IWK44_000851"
     CDS             99..476
                     /locus_tag="IWK44_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549397.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            complement(656..1951)
                     /gene="purB"
                     /locus_tag="IWK44_000852"
     CDS             complement(656..1951)
                     /gene="purB"
                     /locus_tag="IWK44_000852"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP9549398.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1964..3097)
                     /gene="purK"
                     /locus_tag="IWK44_000853"
     CDS             complement(1964..3097)
                     /gene="purK"
                     /locus_tag="IWK44_000853"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP9549399.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(3081..3569)
                     /gene="purE"
                     /locus_tag="IWK44_000854"
     CDS             complement(3081..3569)
                     /gene="purE"
                     /locus_tag="IWK44_000854"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP9549400.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3862..5169)
                     /locus_tag="IWK44_000855"
     CDS             complement(3862..5169)
                     /locus_tag="IWK44_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP9549401.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(5172..5753)
                     /locus_tag="IWK44_000856"
     CDS             complement(5172..5753)
                     /locus_tag="IWK44_000856"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP9549402.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5869..5966)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(6325..7617)
                     /locus_tag="IWK44_000857"
     CDS             complement(6325..7617)
                     /locus_tag="IWK44_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9549403.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7614..8237)
                     /locus_tag="IWK44_000858"
     CDS             complement(7614..8237)
                     /locus_tag="IWK44_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549404.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(8240..9076)
                     /locus_tag="IWK44_000859"
     CDS             complement(8240..9076)
                     /locus_tag="IWK44_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9549405.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(9093..9980)
                     /locus_tag="IWK44_000860"
     CDS             complement(9093..9980)
                     /locus_tag="IWK44_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9549406.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9988..11223)
                     /locus_tag="IWK44_000861"
                     /pseudo
     CDS             complement(9988..11223)
                     /locus_tag="IWK44_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(11906..12589)
                     /locus_tag="IWK44_000862"
     CDS             complement(11906..12589)
                     /locus_tag="IWK44_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9549407.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12607..13224)
                     /locus_tag="IWK44_000863"
     CDS             complement(12607..13224)
                     /locus_tag="IWK44_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP9549408.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(13234..14058)
                     /locus_tag="IWK44_000864"
     CDS             complement(13234..14058)
                     /locus_tag="IWK44_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP9549409.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(14051..14611)
                     /locus_tag="IWK44_000865"
     CDS             complement(14051..14611)
                     /locus_tag="IWK44_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549410.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14785..15741
                     /locus_tag="IWK44_000866"
     CDS             14785..15741
                     /locus_tag="IWK44_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9549411.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(16046..17002)
                     /locus_tag="IWK44_000867"
     CDS             complement(16046..17002)
                     /locus_tag="IWK44_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP9549412.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            17106..17708
                     /locus_tag="IWK44_000868"
     CDS             17106..17708
                     /locus_tag="IWK44_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9549413.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17796..19217)
                     /locus_tag="IWK44_000869"
     CDS             complement(17796..19217)
                     /locus_tag="IWK44_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP9549414.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAALAKGKAEAEAKQKIANAFKEYGEAAVLSMVIDMLPQLMREAAQP
                     LGNIEKISVVDTGSSSGETSGANRVTNYATNLLSSTQETLKATTGLDLKEIVENLSTR
                     GTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(19233..19757)
                     /locus_tag="IWK44_000870"
     CDS             complement(19233..19757)
                     /locus_tag="IWK44_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549415.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            20055..20804
                     /locus_tag="IWK44_000871"
     CDS             20055..20804
                     /locus_tag="IWK44_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP9549416.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20795..21580
                     /locus_tag="IWK44_000872"
     CDS             20795..21580
                     /locus_tag="IWK44_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9549417.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21549..22265
                     /locus_tag="IWK44_000873"
     CDS             21549..22265
                     /locus_tag="IWK44_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9549418.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(22367..23386)
                     /locus_tag="IWK44_000874"
     CDS             complement(22367..23386)
                     /locus_tag="IWK44_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP9549419.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23405..24463)
                     /locus_tag="IWK44_000875"
     CDS             complement(23405..24463)
                     /locus_tag="IWK44_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP9549420.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7317 a         5254 c         4277 g         7709 t
ORIGIN      
        1 aatcttatac catcaacaag cgtttacatt tatacttaat gatggtatat tagaattaac
       61 taaaaatttg gataatgggt tcgaggaggc acttgccgat ggaagatgca ataaaacaag
      121 gtcagctttt tttatcagaa ttaacagctg aagaacgttc ctgtctatat aatgatttgc
      181 tgtcgatcaa agacgtttat ttatgcaata aagatactag tttttataca ttccgaaaaa
      241 tccgccgagc aaccaagcgc tgtgctgaat gccgtaaaag gacttttctt tatcagacag
      301 aacacaaagg cgaactttca atcagtcaga ttcatgaaca gaagcgtttc aaaaatcatt
      361 atcgtatcca gatcttattc aatggacaag agatggaata cgcttttctc gaaaacttgc
      421 tgcaacatct ccataaccag cattttagtt ataatagcca agccatttac gcttaattcc
      481 tctatttttt gtatgtaaag aggcacggta aagaggtagc cgcttttaaa atcaggttcc
      541 tattattgat aagaaaatat aataaactaa aagaagcaga accgctaacg gatctgcttc
      601 ttttttggtt atttttgcta ttctatttta attgaatttt gcaactcact cttctttatt
      661 ccaaacctac tcgtttgaat atcacgtcaa cgtttttcaa atgatgatga taatcaaagg
      721 catcgtctat ttcttcttta gataataccg atgtgatggt tggatcttct tctaacagcg
      781 ggcggaactg cacttggttg tcccaagcgt acgcagtttt tggctgaaca agatcataag
      841 cttcttctct tgtcattccc ttatcgatca atttcagcat aacgcgctgg ctataaatca
      901 aaccaaatgt tgcagacata ttccgcttca tgttttcagg aaagaccgtt agatttttaa
      961 cgatattgcc aaaacgattt aacatgtaat tcaataaaat cgtggtatca ggcaagatga
     1021 ttcgttctgc tgaagaatga gaaatatctc tttcatgcca taatgtgaca ttttcatagg
     1081 cagtgaccat gtgtccgcgc accactctgg ctagaccagc catattttct gaaccgatcg
     1141 gattcctttt gtgcggcata gcagaagaac ctttttgacc tttcgcaaaa aattcttcta
     1201 cttcacgtgt ttcggatttt tgcaatccgc gtatctctgt tgcaaaacgt tcaatacttg
     1261 tagcaataag cgccatcgct gagaaatatt cagcatgtag atcccgaggg agaacttgag
     1321 tagagatttc ttgtggacgg atccccaatt gtttacatac atactcttca ataaatggcg
     1381 ggatatttgc gaacgttccc actgctcctg agatttttcc tgcttctact ccttttgccg
     1441 catgttcaaa tcgttcgatg ttccgtttca tttctgaata ccatgtggca agtttcaatc
     1501 caaaagtagt tggttcagca tggacaccgt gtgtccgacc catcatcact gtatatttgt
     1561 gttcttttgc tttttcgcca atgatttccg taaaacgttt caagtcttcc cgtaaaatat
     1621 catttgcttg cttgatgaga tagccataag cagtatctac gacatccgta cttgttagac
     1681 cataatggac ccattttctt tcttctccca atgtttcaga aaccgctcgt gtaaacgcaa
     1741 cgacatcatg gcgtgttaat ttttcgattt ccaagatccg atcgatatca aatgatgcat
     1801 gctcacgaat ttttttcaca tcttcttgcg ggatttcacc aagttctgcc caagcttcat
     1861 ctgctaaaat ctcgacttcg agccacgcac ggtatttgtt ctcttccgtc cagatattcc
     1921 ccatttcagg gcgtgtataa cgttctaaca tgttttttct cctctaatcc caaatattcg
     1981 tttgtttgat ttcttccagc gtttgctgga tgtcttctgt caagatagtg atatgtccca
     2041 tcttgcgacc tttttttgct tgtttttttc catagaaatg gaattgccag tctggcttat
     2101 tctcaatcaa gcgatagctt tccagcagtt gttctcccaa aacattgacc attactgcat
     2161 tgcttatcaa acgtactttt tctcctaatg gccaaccaca aatacctcga atatgggcat
     2221 cgaactgact catagaacaa gcttcaatag aataatgtcc agaattgtga ggacgaggag
     2281 ctaattcatt cacatatagc ccgcctgttt ttgttaaaaa catttctacc cctaaaacac
     2341 ctgaaaggct cactgcttct gcaatcacac gggcaatacg ctcagcctcc tcgatcacat
     2401 cctgatcaat cgcggctgga gcaatcgttt catgaagaat attgttgtgg tggatgtttt
     2461 ccacgaccgg aaaagtcgtg aaatctcctt gaccatttcc tgccaccatg atcgaaatct
     2521 ctttttcaaa cgggatccat gcttctaaaa cacaggttcc ttctcttaaa aggtccatgg
     2581 caggagctag atccgcccga cttttcaaaa catactgccc cttgccatca tagccgcctc
     2641 gagtggtttt caaaacacaa ggatagccga tcccgtcaat cgcatcttgg atatccgtcg
     2701 gactgacgat cgttgcataa ggagcaatca cgatgttatt cgtttccaaa aaagattttt
     2761 ctaacaggcg gtcttgcgta atcgcaagca agtctgtccc ttgaggaata aatgacatgg
     2821 gtaaaatcgc atttaacgcc tctacactga cgttttcgaa ttcataggta atgacatctg
     2881 ttcttctggc aagctctttc agcgcaaagg tgtcatcata gacactttca atgtgccaat
     2941 ctgcaacttg tgcggctgga caatcgctca caggatccag cacacctact ttaaagccca
     3001 tttcttttgc actaatcgcc atcatacgac ctaattgacc gccaccgacg atcccgatcg
     3061 ttgaaccagg caacaaaggt ttaaccaagt tgatcactac tttctataac agttttagcc
     3121 aacattgaac gtctttcagc caatttagcc tccaattcta aatcatacat ggaaagcatc
     3181 tgtatcgcaa gtagtccagc attaattgct cccgcttttc cgattgctgt agtagctaca
     3241 ggtactcctc caggcatttg gacaatcgaa agaagcgaat ccaaaccatt taatgttcgt
     3301 gattggaccg gtacaccgat aaccggcaaa gttgtttttg atgcgaccat acccggcaga
     3361 tgcgcggctc cccctgctcc tgcaataatg attttgattc ctttttttcg agcgttttgt
     3421 gcatattcaa acatcaaatc tggggttcga tgagcagaaa cgaccttttt ttcataagac
     3481 acaccgaatt catccaatat ttgacatgct tctttcattg tttcccaatc tgaagtactc
     3541 cccataatca cagagatttt tgctgtcatt gtttttcctc ccagtttttc tacattcata
     3601 ttttaacaat cacttttttt gatgtcaaag aaatttcgaa tgttaacaaa gaaaaacatt
     3661 ttattgttcg tgtttattaa agatgaaaaa aaggaatgtg acaaaagtta ggtcacattc
     3721 ctttttatgg ataaacggtg ttcaatcagc tgaccctcgg tattagctca aacaaacggc
     3781 aaaatacgag gaactatttt tccgtttgtt tctctaacaa tcaggtcaaa acgctgattt
     3841 cacaaccttt cttttgctct attacttctt gaaggcttct tccaatattt cttcatcttt
     3901 tttcaatcca ttaaagatca aattcaatat gatagccgta atgctactca ttacgatgcc
     3961 atttccagtg aacatttgca atgtttgcgg catttgactg aataatgtcg gcgtaacatt
     4021 gaaacccagc ccaaatccaa cagaaacagc aacaatcaat agatttttat cattgctgaa
     4081 atcaacttcc aacagcatac ggattccttg gactgccacc atcccgaaca tgactaacat
     4141 tcctccacca agtaccggct cagggatgat ttgtgctaac gcaccgattt tagggaataa
     4201 tcctaaaaca atcaagaaga atgcagagaa atagatgggt tttcttgttt tgattcccga
     4261 aagttgaacc aatccgacat tttgagaaaa gccagtataa gggaatgtat tgaaaatacc
     4321 gcctaagata acggcaagtc cttctgcacg atatcccttt ttcaattctt cttctccaac
     4381 atttttccca gtaatgtcac caagtgcaaa atatacacct gttgattcta ccatgctgac
     4441 catggaaatg atgatcatta gtaaaatcga ccatacgtcg aaagttggtt taccaaaata
     4501 aaagggttgt ggaacatgga acaacggcgc atctccaatc gcgctcagat caatcattcc
     4561 catgaaagca gctaagatgc ttcctccgac taaaccaatc aataccgcga ttgaacgaat
     4621 gaaccctttt gccagcattt gcacgcctac aattaggaga atcgtcacaa atgcaagtag
     4681 cagatttgtt ttgtcaccaa aatcagctgc tgttgcatta cctccaccca ttttctcgat
     4741 tgctacagga atcaaagtca aaccaataac ggtgataacc gttcctgtta ctataggagg
     4801 gaatagtttt ttaattttag aaaagacacc agaaactaac acaacaaaga tcccagaagc
     4861 gataatagat ccgtagatag ctcctactcc ttcattggtt ccgatcaaga tcagtggagc
     4921 tactgcttgg atcgcacatc ctaaaacgac aggcaagcca attccgaaaa atcggttaac
     4981 tgtcaattgc aataatgtcg ctaatccaca cataaagata tcaattgaaa tcaaataagt
     5041 catctgttct tgattaaagc ctagtcctgt accgatcaaa agaggcaccg ctactgctcc
     5101 tgcatacatc gctagcagat gctgcagccc taaaaccgca gcttttccat tttgtgtttc
     5161 tttttccacg attatgcgtc ctcctcaaga aactcaactt gtccattcgt caaggaagca
     5221 atacgcgcaa gagaaacgac tctcaatccc atttcttcaa gtaactgacg tccatcttgg
     5281 aaagactttt cgatgacgat cccgattcct tctacttgcg ctccagcttg ctggcataat
     5341 tcgatcaatc cttttgccgc ttgtccgttt gccaagaaat cgtcgataat caatactttg
     5401 tcttctgaag ataaaaattt acgagaaata gaaatcgtac ttgtcacttg tttcgtaaaa
     5461 gaataaacag aagctgtcaa taattcttca tccatcgtca aactttttgc ttttctagcg
     5521 aagatcattg gaacatctaa ttctcttgca gcaaataaag ctggtgcgat cccagaagct
     5581 tcaatggtca caactttcgt gattccttgt tctttaaata cttctgcaaa ccgttttccc
     5641 atttgttcca tcaatacagg gtctacttgg tgagtgacga agctgtcgac ttttaatacg
     5701 ccctcgccta atatacgtcc gtctttttga atacgttcaa ctaattcttt cactttattt
     5761 gctcctttat catttattaa taccgaaata accgcttttg tgcaaaaacc tactatgttt
     5821 ctgttaaaga aaaacaccct ccactttatc ataagaaaag aagggtgaaa tagccaatct
     5881 tttacttata gtccagcatt tacggtactg gctagaaact gtcatccata ttatgacgat
     5941 atataagtag gtatgtaaaa ctaataatag tgtaaattca ttttcttgca actgttttgc
     6001 acgtgaaggc agtttgccat atttgaataa aaaatgttgt ttcaaatatt gaaaacagtc
     6061 taacactact ttctgaaact gacaacgtgg tttacttatt tttttgtaaa atctaatgat
     6121 ttttaatgca ttattccttt ttgttcgggt ttaaatagtt atttttattt tctcctatct
     6181 aaaaaagcag cccacataaa aagaaggcat gacaaaaagc atgtcatagc ccctttttcc
     6241 gaataaatag tgttcaaaag acagcctatc ggcaataagg caagatttta taaactacgg
     6301 gaagtaattt ccgtaaatct attcttattc ttttaagctg acaagctcgt caatcactac
     6361 tgcttttcct gaagcaatag aatgatttgc tgcgattcct gtcaaaatcg acaaaatacc
     6421 atctacgtga gttgctgccc gattccacgg atcttcttct ttttcttcac caaatagatc
     6481 atctaataaa cgcggatctc ctccgccatg tccgcccgct tcttgttcga cgattacctc
     6541 atatggctca ccgaacatcg gatagacatg gatggactga agaacggctg ctccttcatc
     6601 ctttttatct ccacctgcat tgatgtaaga cttttcgatc actttcattt cgatccgtcc
     6661 tttgctgcca ttgaaagaaa cattgaatcc ttcccacggc atatacgcgt ttagcgaata
     6721 cgtcaaaatc gcaccagttt catatttgac cgttacgcct agtgtatctt caatagagat
     6781 atcatcggaa aatacactgc ggtcacgaat atatccactg tctttttctg cttctaaata
     6841 catttctcgc aattcctcat tggctgacag gtctatagca aatggatcag ctttagctag
     6901 ttcgcttcca tagcatcgac tgtaaaatat ttctactccg cgtttctccg cattatgcat
     6961 cccataaaag tttaatccac cgaatgcaaa taccttttgg ggacggctgt tcaaccaaaa
     7021 attcactaga tcaaaatgat gggtagattt atgaacaaga agcccgccgc tattgtgctt
     7081 atttcgatgc catcttctga aataatctgc cccgtgttgc gtatttaaca gccattcgaa
     7141 gtggatagaa aacacatcac caatcacatc actttctatc agttccttta tcttcgtgtg
     7201 atacggtgca taacgataat taaaagtgac tcgcaggctt cttcccgtct gtttttggac
     7261 cgttattatt tcttgagctt tttcctcatc gatagtcaga ggtttttcgc aaataacatc
     7321 atatcctttt ttcattgcac gaatgatata atcatgatgc gttcgatcta ctgacgtaat
     7381 aatgacttca gccggccgta cgtcatccag tagttgctca aattgtgatg gcatacatgt
     7441 ctttactttt ttctgtccat acttgttcac aagtatccga tttgcataat ccatccttgt
     7501 ttgagataca tcacaaaaac cgactagttc acaagtttga tgatacgtag aggcaattgc
     7561 ctcataaaaa aaacgcgctc tcccaccaag tccgacttgt acatattttt tcttcattta
     7621 aagtcattct ccttttcttt taagcaaaat actgtgacaa atggcagccc tggatacagt
     7681 aatgcagtga gtaaaaaatc aggctgtata caccagaaca gtccatacaa aaggcataaa
     7741 taccacagaa accccttgac gttttttgac aacagccaga tattcctttt gatcaaataa
     7801 acaagactat ctgctttatc ttctttgcag atatcaatta gcgtactaaa aaaactatgg
     7861 gatacaaaaa gcaacagaca caatagaaaa ggaatcagat accaagctcc aatctttccc
     7921 actaataaat accaatcaac tgcaaaaata atgacaaaca gctgaaacaa cgtaatgatg
     7981 aaacacatcc gtttgatttt ccctttcctc caacatacct ttttttctgc caagtaatgg
     8041 acagatagat aataagaagg atacatcatc aaaaaataaa tcagatagac aacacgagca
     8101 gacagcacta atcctaaagt gaacaaacat cctagaacta aagcgaaaag agcattactg
     8161 ataagactgg ttaaaaaaat ccgacagaca tagtagttga gcttgtaaag aaacatctct
     8221 tctattggct ttttcatatt cagcccttca gaccgcttgt actgatcccc tggacaagat
     8281 atttattgaa gaataagaaa atcagaaaaa ccggtagcag cgacaacgta gacatcgcaa
     8341 acatactcgc gtaagtcgta ctagatccag cgtctgcaaa taatttgatg gcaatcgata
     8401 ccgtgtaaga tctcggcttg tttaaataaa tcaatggtcc catgaagtcg tcccatttcc
     8461 aataaaactg gatgatgatc gtcgtgatga tcgaaggttt cagcaaagga aaaataattt
     8521 tggtaaagac actatacttg ctgcatccgt caataatcgc tgattcatct aactctttgg
     8581 ggatgctgac catgaattgc atgatctggt aaatgaaaaa tgcttgtcca aaaaaatgtg
     8641 gtaaaatcag cggcacatac gtcccaacca aattcagccg attgtaaata ataaactgcg
     8701 gaatcaaaat cacttgtgca ggaatcatca tcgttactaa catgacgacg aaccagaatc
     8761 tttttccttt gaaatcgatt ctcgataatg cgtaagcaat acaagtcgag ctcagaactg
     8821 tccctatcgt cgaaactgca gagatgacaa acgaattcct aaagaaagta aaaaatgaaa
     8881 ttccagcaaa ccccgcccat ccttttgcgt aattttccca ttgaaaagtc tctggaatca
     8941 aattcagcga accactcata atctcttgat ccgttttaaa agaacctgcg atcatccata
     9001 agaccggata aatcatgact agacctaata gcaaaataaa accgtattga aaccatttcg
     9061 ttactgtatt attcatttct tcctcctctc attcacacaa cgacccattt ttttgatgat
     9121 ttgaaaatca gaaaggtgac agctcccata atcaacagca tgatccacga aatagcacta
     9181 gcatacccca tagaattaaa agaaaaagct tggttataca cataaagagc ataaaagttc
     9241 gtggcatcat tcggaccacc ttgggtaatg ataaatgcct gcgtaaaagt cataaacgcc
     9301 gcgattgtct gcataaccaa gttatacaaa atgatcggcg aaagaagagg taacgtgatc
     9361 ttgtaaaacg tctgccattt gttcgctcca tcgatctctg ccgcttcata atacgagcct
     9421 gggatctctt tgatacctgc agcaaagatg atcattgccg aaccgaattg ccaagcagac
     9481 atcaaaacaa gagggatcat gaccagtttt tgatcgctaa accaggaaag cgcaggtgcg
     9541 cctaatcgtt ccaggaaaat attcactaac ccgtttttag aaaacaactc ccgccaaact
     9601 agagcgatag aaatactgcc accgatcaaa ctaggcacat aatacaaaga acgaaagaaa
     9661 gtcgttcctt tgatattcct cgttaaaaga taagcaatca atagaccaaa gatcagtttc
     9721 acaggtacag atgcaaatac atagagaaaa gtgactcgga tggagttcaa aaaacgggca
     9781 tcactcagca tctttatata attatccaag ccgatccatg agactggatt tcccaaacga
     9841 taatcggtga acgaataata aagtgacaag atcatcggta tcactgttat caatagaaat
     9901 cctaggatga atggagaagc aaatacgtaa ccgaccacat tgtcttgatc caagaaccgt
     9961 tttagttttt cttttttcat gccttccttc tgctgttact tcttcataaa tgactttttc
    10021 aactaaaaga ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac
    10081 ttcgccgttg tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc
    10141 atcatctgca cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc
    10201 ttttaaaatt tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc
    10261 ttctttatgg gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc
    10321 agattctcca tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg
    10381 agcccctgac aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct
    10441 atccctaacc gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg
    10501 atgggcccct ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg
    10561 attttcaaat cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc
    10621 aacttgtggt atttgaataa tacagccagc tataaaatct tcgaatcttg aaatagcata
    10681 gattcctaat tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt
    10741 tgaagctggt tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa
    10801 cgaattcacc ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata
    10861 ggattcatct gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc
    10921 aatctgattg atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc
    10981 tgatgctgct agtgtaccta attttgtgta atatccttca taatcataaa attcataatc
    11041 gatcgtcaca tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg
    11101 tcggacttgc gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc
    11161 acaagccgtc aagaagaata acataacact taaagctaag atgattctca ttcttttctt
    11221 cattctctgc acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac
    11281 atatttatta aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg
    11341 cctaggatga acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat
    11401 cgctaaaaac acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat
    11461 cagacaactg cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct
    11521 tccctaaaaa acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt
    11581 cgatgcacaa gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc
    11641 ccgacaattt agctgctact aaattgtcgg gtgccttttt tagtttttat tcgtttttca
    11701 ttctatagaa agcgcttcaa taaatcaaga catttttttg ttagaatgct aataataaca
    11761 aaaggtttta actcttctta tgtacattac aggtaaaaaa acaaaaaaat ttctcttttt
    11821 tttgaattct gaacaaatag tactggagta caaaagacga ggaagagcca ctatctgctc
    11881 ttcctcgtct tttgattctc acctgttatt catgattctt tttctcagtt tcccgatatt
    11941 tttccatttt acgactaaaa agctctccgc tacttggtgg cgtatatgtg atggcatttg
    12001 ctcctgcttg aatggtctca gcaatacttt cttccgttgg accacctgtc gcaatgatag
    12061 gaagttcagg atatttttta cgaaaatggc gtactgcttc tgcagtttct ttacctgcac
    12121 tgatattgat aatcgaaata ccagctgcta acttttcatc tatttctgta tatttcgacg
    12181 ttaccgtact gataactgga atatcaacta ctcgacaaac ttcttttact gtctctaaag
    12241 gcgtaggacc gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac atgctcatgt
    12301 aggaagaacg agcaccttgt gtcaaaccac cacctacacc ggagaaaact ggaatgtcag
    12361 ctgcctcaat aatgctttta gtaatcgctg gatgaggtgt aaaaggatac acagcaatta
    12421 ctgcattcgc atttgtatta cgtatgatcg caatatccgt agtaaagatg atcgaacgga
    12481 tcaattttcc aaatatcttg atcccgcttg cttctaaaat gatttccggt actcttacga
    12541 tatcttttct caattccgtc atgatttctg gcgtaaattg ttctgccatc agccacttct
    12601 cctttgctat ctccggtcat actgattcaa ttgatacgtc tcgatgacct ggatgatttt
    12661 ttgcgcataa ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg cggcttcctg
    12721 atagttggtg gccgtaatca ctttttcata cagcgcagga ttccaatcta ctccttggac
    12781 gaacagttgt gtatggtcat ccatggactg ttcccatgaa tcatacactt tgaagtctcc
    12841 ttggatagtt atccattctt cgttaacaaa ttcctttgtt tccaggctta ctttcttctg
    12901 atccccataa gccttgatcc cgaaaagatt attgtatttg ctagccaagg tactttttcc
    12961 ccaatttgat tctaatatag cttgtcctaa aatgatacta ggaagcactc cataaccatc
    13021 ttgtagttct tgggcatgcg gaacaagccg ttcaataaat tgctgatggg attgcgcttc
    13081 attttcttgc tgattatcag taaaaggaga agacagattc ttcaaggaaa aaacaaaggc
    13141 taagagtatc actgctacac cggcaaccac tgctggccaa cggatattct tgcgcttttg
    13201 ctttttccac ttttttttcg ccatgatcaa ttcctacttt tcatttaatt cattcagata
    13261 ttcttctagc gtcaattggt gtttggtgat atactcagcg ttttctttac cgacataacg
    13321 taagtgccaa ggttcatatg tgatcttagt tatatcttcg tgtccttttg gataacgaac
    13381 gataaatcca tactgcgcag cattatctgc gatccattta gctccaggct gatctccgta
    13441 acttgcatcc aatacagtag aaggataact gttgtaccag ttttgatcga cgacatcgac
    13501 tgccagacct gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt tagtaatctt
    13561 tgtcgcctcc tcttctgata caccttgacg actcatcaca tcttggacat tttgttcgaa
    13621 aacttgttgt tgggatgata cagaccggta agctgagacc atcactaatg gaaatccagc
    13681 tgcttcagca gccttggcaa atgcttcata ttctggagca atgcgtttgt cgatcaagta
    13741 tccgttactt aatgatgcca attgagtaga ctcatcaatt tctttttcta atttgtgatc
    13801 tggaccaacc aaaactagct tccaatcgtc tgttttaaca tctggcaatt gtttggacgt
    13861 acgatctttt gtttctttgt cctttacctt gctgctggtt gtttgactgt tcttttcttt
    13921 actagcagtt gttttggtgc ttgttcgttc gactgcttga ttgccggctt gaacatgcga
    13981 atcatcatcg ctaaaaataa catagcccat tgctgcgatc ataatcacaa ctgctccaaa
    14041 tcctaaaaat ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc ccattcgcct
    14101 catgaacgta agtttcttcc agattttcat tcacccaatc gagaagttcg attttttctc
    14161 cttcgatctg tttttgaaca tctgctggca gacgaaaatt caaaatatca tcatagcccc
    14221 attcatcata ttgaatactt actttcaagt caaggtatct accagcctgc tgattatttt
    14281 tcaaagggat cagctcgact tttgctgctt tgctgctaag ccatttttgc gcgatgcgtg
    14341 ttttgatttg cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa ataatcgttt
    14401 gacgcaaaat cttttgcgtt agcatccatt cataatcagt gaaaagattt tttactttct
    14461 gcatcgcttc agatggcaga tttttctcgc catctttcca tttttgccat tgttctggac
    14521 tgatcagcaa gtattcatct aaaaaatttt tttcagtttc aaactgttct aataatacac
    14581 tcataataag atcaatatat acttgatgca cgtttatccc ctccttttaa ctattctact
    14641 taagttttga tcaaaaagca cctcttaaat cgctgctttc atttttttta atgtttcaag
    14701 tcctctcctc tttattcttc gtgatttttg ttataataat agacagacaa gaaactcaat
    14761 ccaactatga aggagcgttt tcagatgaaa aaatatcaat ggggaatcgt tggtcttggt
    14821 accatcgcac atgagtttgc cgaaagcttc aatcaagaga cgagcgaact aacagcagtt
    14881 gcttccagaa cttcagaaaa agcggaaaac ttcgctcacc gttacaatat tccaaaagct
    14941 tacggaagct atcaagaaat gttagacgat gctgaaatcg acatcgtgta cattgctgta
    15001 ccaaatagac agcatatcga ccatatcttg gcagcattgg aagcaggcaa acacgttcta
    15061 tgtgaaaaag ccatcacgat gaataaaaaa gaactggctg acgctatgcg cttagcagaa
    15121 gaaaagaatt tgatcttagc agaagcgatg acaatcttca acatgccact ttatcagcag
    15181 ttacgctcaa taatggatac aggaaaactg ggggctttaa aaatgatcca agcacctttc
    15241 ggcagttaca aagaaccaga ccctaaaaac cggttcttca atccagaatt agctggagga
    15301 gctctgttgg atatcggtac gtatgctgta tctttcgctc gtttcttttt gagctctcaa
    15361 cctgaagtag ttgcctctac aatggttcct tttgaaacag gcgtggacga acaatccgtc
    15421 actatcttgc gtaacaaaga aaatgaactt gctgctgtca gcttgacttt tcaggcaaaa
    15481 atgccaaaag tcggtatcgt tgcctttgag aacggttatg tgacgattgc tgattacccg
    15541 cgtgctgatc gtgcggagat cttatttaca gatggtacaa aggaattcat cgaaagcggg
    15601 aatacatctg aagcaatgaa ttacgaaata aagaatatgg tacacatgat tgaaggtgac
    15661 ttgcccaatc gctctctctt cttgacaaaa gatgtcatcg aaatcttgga tcaaatgcaa
    15721 gttctatgga aaaatatgta attttaaaag gttgtgaaaa agctgttctg catcaagtaa
    15781 taagaacagc caaacaaaaa tagctcctcc tatttttcgt ttggctggac ttattaccgc
    15841 agaggaaagc ttttgaacac cgtttaagaa agaggttgtg agcctaccgt tcagctccga
    15901 gtcacaagga gcaatttcaa aaaacagctt ctcatgtttg ttgaatattg tgacttgtga
    15961 ccgaggagtt gacaggagaa caccgtttaa agaagaggtt gtgaaatcag ctttttgacc
    16021 tgaatattag ctgaattcac aacctctatt tttggatttt ttgttgggag aattctgcct
    16081 tgatttcaat aaaactttta aaagactgca aaaactggaa caattctgct cgatcctcga
    16141 attcagggcg actagtcggc agaggcattt gccgatacaa tacataaact tcttcaatac
    16201 gtgttaaaat ttgtctgcca tcatttgact ctgaaaatgt ttctgcagta taaatcaata
    16261 aaccatagat atgttttcca taataagctg gttgctggat tctctctaga ttttccagca
    16321 tgtctttaat cacattggcc tgtgctcttc tcatagagaa gtaagtttcg tagtatagcg
    16381 gctgtctgag ccagtagttt tcttgatgtt ctctggcatc ttcttggctt tcgcgaatga
    16441 atgtcagtaa atgttcgcat ttgatctgca catcttcttt attttctgat aaaagaaatt
    16501 ctgccatttg tctcaaaata tttcgaaatt ctttttcgat ctgtatttgg ttttccttca
    16561 gctgcttcgt acgatctggc atgtaaacat ttgcaagcaa agcaaatact aatccaatcg
    16621 ccattaatcc cgcttcattg ccgatcagct gccaagacat gttttcctcc actaaataat
    16681 gagtgaccaa aaccgaattt actactatcc cttcggttaa tttgaagcga gaggcaagcg
    16741 ggatgaacag gaataaaaaa agactgaagc caaaaatagt atacccaaca gcaaaaaaac
    16801 tgaacagtgc tgttgccgta gctaatataa aggctgcgat tcgattcatg cccgtacgca
    16861 atgtagagcg cttggtattt cctacactta ataatgcaat gatccctgca gctggccaat
    16921 acaacagcga taagctaccc gctgtgacca tcgaaaagaa gacactgatc actgttttga
    16981 ctgttcgcat tcctatacgc atttacttcc ccctttttta ttttaattat acttgaataa
    17041 tgaaaaacta gctatcagac aaaagtttag gtatgatagt aaaaagacca aaaaggagaa
    17101 tctctatgca atttggccgt tttcgactag gtatgcgtac gatcaaaagt gccttagctg
    17161 tttttttatg tatcttgttt tttcatttga ctcaacgagg aatcccgatg attgctgcat
    17221 tatctgctgt tttttctttg cgtcaggact tgactacaac cgtttctttt ggaaagtcta
    17281 ggatcatcgg caatacatta ggtggtacat tagctatcat ttattttttt gtcaaaagct
    17341 attttcacaa tgattttttg gtagaattga tcttgcttcc tattctagtt attattgtga
    17401 tcgtcgtgtc agatggtatt aacaataatt ccggtatcat ttccgcaata gctacgttgt
    17461 tgctgattgc gttgagcatc cctcaaggcg aatccgcttt gtatgctatc caacgtgtac
    17521 tagatacttt tatagggaca ttcattgcaa tcggtatcaa tttctttctt cgtcctcctg
    17581 aaacagaaaa gaaggaagaa ctatcagaag atttggtcga actccaaaag aaagaacgat
    17641 tgctaagaac aaatttacaa gagatagaag ctaagatcca aaaacaaaag aaaaatagcg
    17701 aggactaatt ttcttttgca ataaaataaa aaaatggaga gaagaacttt aaaaagtctt
    17761 cctccatttt tgattgaacg gtgaagtgta ctactttatt cttccttcat cgagttttgt
    17821 tctgcctctt ttggcgcttc atacatattt acttggctag tagtcccacg tgtcgacaaa
    17881 ttctctacga tttccttcaa gtcaagtcct gttgtcgctt ttagtgtttc ttgagtagaa
    17941 gaaagcaaat ttgtggcata atttgtcact cggttcgctc cactggtttc tcctgaactg
    18001 cttcctgtat cgacaacaga gattttttcg atatttccta gcggctgtgc tgcttcgcgc
    18061 atcaattgcg gtagcatatc gataaccata ctcaagacag ctgcttcgcc atattccttg
    18121 aaggcattgg cgatcttttg ttttgcttcg gcttctgctt ttccttttgc tagtgctgct
    18181 tcggcttcag tcacttcttt gactttttga gcttgtgctt cttgttcttt agcgtaccga
    18241 tcggcatctg cttttttctt cacttccgaa tcatattgct tttcacgacg tgtgatttct
    18301 ttttcttcca gttcgatttg tttttggcgt tctacgattt taatctgcat ttcctgttcg
    18361 attacttgtt gttgtgctcg ggcactttct aaattgtagg cttggtctgc ttttgcttta
    18421 gcaacatctt gttcttgttt gtaggttgct aatttcagtt ctttttcttt taatgattct
    18481 gcgatctctg tttgtcgctg taattcagct gcttgtgatt ctttttctgc ttgagctttt
    18541 ttgatacgcg tttcttttag agcttccgct tctgctatat ctgcatctct tttgacttgg
    18601 gcgattcttg gtttccctaa agagtcaaga taaccgtttt tgtctctgac ctctttgata
    18661 gtgaaagaaa cgatgatcaa ccccattttt gcaaggtcta cagaagcgac ttcttgtacg
    18721 ctttgactga acttatcgcg attttgatag atttcttcta cagtcataga accaagaatc
    18781 gagcgcaagt gtccttctaa tacttctctt gcttcatttt ccagttcttc tcttgtcttg
    18841 cctaaaaact gttctgctgc tgtagcaatc tcttctacag atgaaccgat tttgatgatc
    18901 gaagtaccat cacacatcac cggtacgcct tgttcggtat acacttcagg ggtggagacg
    18961 tccaatttac tcgataatag actgatccga ttcgaacgtt gaaaaactgg caagacgaat
    19021 gcgccacctc cacgcacaat tttgattttg ttattgcttt catcaatgtg cacatttttg
    19081 cttcctaaat aacttccact gatgatcaac gcttcatccg gttttgctgt ctggtacttc
    19141 gctacaaaga cgatcagcaa catcagtaaa atcaaagctg cgattgcgat cgcgataaaa
    19201 acaactggta tttccattta acacacgctc ctttaagctt ttatcatgct ttcataaggc
    19261 atgacataac agattctttc cttgatctca atgatcaata ctttcgttcc ccgctcagct
    19321 gtctgttctt gggggctgta aaaacaagct ggacgactaa tggaaccggt aacacttttc
    19381 aactgaattt ctcccatacc tcgtacaggg atcggtgtga tcacaacagc tacttgtcct
    19441 tccagagttt tttcagaaga cgacatcgtt gattcggcat gtttcatcgg catcaatata
    19501 tagaaattaa gcaaaaatac caacactatc gatatgcctc cactagcaat aaataaaaat
    19561 aaagatgatt gatctgttaa acgctcacct agaaaaccaa ataagcttgt gaaagttaac
    19621 caagggataa taagcatcgg atcaagcggg ccatcaaaat cgaatacatc tccaaaaata
    19681 atcaatagga ctgccaatcc tgcacaaatc agcaatgtat accaataaat cgtctcaagt
    19741 gcgattcccc ctatcatata cacgcctcct tccatttcta cgtctatcat accaatgttt
    19801 gaaaattctg tatagcttct tttaccgact cagtggtgtt ttttatagga aggttgtgtt
    19861 tatcgttgac aatttgttaa atttttgttt tcatgttaaa attttcacaa taaattcttt
    19921 cgaggtagcc tatgtttcct ttttttaaga aaaaaccatt aaagctccag aacacgatca
    19981 tccttgtcgt ttgtttctcc acattcgttt ctttgattgt ttctgccata ttgatcagga
    20041 attttattct ttcgatgaac cgatctagtg tgtcagataa aatctctagc attgctaaaa
    20101 tcgttgccaa tgataaactc atccaaaaaa agctgcagac tggtgacccg acacatgcga
    20161 ttcaaggaat aacaaatgaa atcatgattg catcgaattt agattttatc gttgtcatgg
    20221 accctgaagg cattcgatat tctcacgcag acccaagtta catcggtctt cctttctcaa
    20281 gtcatgaaga tgtgaaaaaa gtgattggag gacaagaatt tttctctgaa aaagaaggaa
    20341 tgttagggtt cggcttccgc tatttcgtcc ctatttatta tgatcaagag attattggtg
    20401 cagtttgtgt tggcttaaca aacaacacga ttgacaatca agtcaaagat ggacaaaaaa
    20461 tgattgtttt ttcattgatt attggactta gcgtaggcat cataggcgcc gtttttcttg
    20521 cccaaaaaat gaaaaaagta ctgcttggac ttgaaccaca agaaatagca gaacagttaa
    20581 gtgaaaaaac gattatcgaa aatgaagtag atgaaggaat cttagccatc tcttctgata
    20641 aaactatcct cttgatcaat acacctgcaa aaaacatttt ctaccaaaca gagcagctat
    20701 tggatttcga agtaggaaaa aaatcagtga taatctatat gacgttttgt ttaaagagac
    20761 gattgagcat caaacgaccg ttcggactca atccatgtat ttaaatggga tagaaactat
    20821 ttctagcgtt acacctcttt ttagcaatga ccgtttttta ggagcagtcg caactttacg
    20881 aaatcgatca gaagtaatga aattaatgga ggagcttggg gggacgaaac aatatataaa
    20941 ctccatgaga gctcaaaccc atgaattcat gaataaactt catgtcattt ctggtttgat
    21001 tgatttgaag aagtatgaag aagtcaccca ttttatccag cagctaaatc aaaattataa
    21061 aaaagagatt ggccaagtct caagtctgat caaagtacct gcaattgccg gttttttatt
    21121 aggcaaaata aacgaagcca aagaacaatc tgttgaaatg ctgttagaaa gcgattcttt
    21181 tatccctaaa atggagatgg atgactccat ccattcactt ttgcaagtgt tagggaactt
    21241 acttgataat gcaaaagaag ccgtgctaca aaaacatgaa agaaacggac aggtccggat
    21301 tcatttaaac tatgacacag aagctgaagt attcatcgtg accgtctgcg atggcggtgt
    21361 cgggatatca gaagaaatct cagaaaaaat atttacgcta ggtttttcga cgaagggaat
    21421 caatcgtggg tttggattga atctcatcca ttcaatcgtc aaacgccacg gtggattttt
    21481 agaattttcc aaacaaacaa atggcggtac aatcgtctat ctagaattac caaaaaagga
    21541 agatcaaaat gatgacaaat gtcctgataa tcgaagatga tccaatggtt gcagaattga
    21601 atcgtacttt tgtggaaagc atccctaata tgaacgtaat cgaaagtttc agaaatgtag
    21661 aagaaggaaa aaaatatttc agacaaaaaa acgcagatct gctgctgctt gatgtataca
    21721 tgcctggcga aagtggtatc cattttttat cttggcttag acagcaagga aatgatatcc
    21781 cagtgatttt gatcactgct gcagatgata tcgaaaccat aaagaatgtg atccgctacg
    21841 gtgccttaga ttatttgatc aaaccgttca ctttgcaacg ttttcagctg gcgatcgaaa
    21901 agtatcttgc tttctctcag acaatccaaa cgactgaaaa agccgatcag aaattgatcg
    21961 atcaagtatt caaccacacc actcccattc acgaaatacc atcattcatc gatttgccta
    22021 aaggtctgtc aaaactgact ttaaaaaaaa taagtgcatt tattctaaaa gaagatacta
    22081 ttttttcaac aaaagaactg gctcaaaaaa gctcactgtc acgtatatcc gtcaagaaat
    22141 atttgaattt tttagtagaa caagatatat tgaacgaaag aatcaaatat ttggaaatcg
    22201 gccagccgct aacctgttat gaattgacaa agaatgggcg gaaaaagctc acagattact
    22261 gttgagtcat cgaattatcc catacaaaat agtccaagta tacagaagta acaactgttt
    22321 acttggacta tttttaaaca ttttattgac gaatatccat gttttcttat cttaattgac
    22381 gggctttagg aagagatacc tcatttttcg tttttccagt ttctattatt tcacgaaagt
    22441 tctcgaaact tgtcgaaatc atatttgata atgcctcatc tgtatttgaa cctacatgcg
    22501 gggttacaag aactctaggg tacatttcta ctaattgctg gatagcaggg tctggaatcg
    22561 tctcccatgg cttgaaggat cggaaaaaaa cctctgtttc atttgcaaaa acatcggttg
    22621 caaaaccagc taaatgattg cttttcaaag ctcttaaaat agcttcatta tcttgaagct
    22681 ctccacgggc tgtattaatc aagatgctac cttttttcat ttgagctaaa aatccttcat
    22741 tgatcatttg atcgttttca cctgggatat gaggtacatg aatactgaca atatcgcttt
    22801 tggctaataa ctcttcaagc gagtggaacg tcaatacttt tttagcagct tctgtctgat
    22861 gaatatcgta gccgatcact ttggctccta gtcccttaaa caaaacagct tccgtcaacc
    22921 ctatcctgcc tgttcctatg atccctactg tgcaatttct gatttcacga ctaaacattt
    22981 gctcatccac tcgaaaatct ttgaaagatg ttcggatcgt cgtatacgca gtatttctca
    23041 aaagcatcat ggcaaaagtc aaagataact ctgctatcgc atttggcgaa tacgatggta
    23101 cacgggctac tgtcatacca aaatcagctg ccgcttgtag atcgatatga ttgactccca
    23161 cagtccgtgt aaaaacataa cgaatcccat attcctgcat ttttgcaaga ttggtctcat
    23221 ctgctataca atttcccctc aacaatacag catcatggtc ctttgctgac acgatattgt
    23281 catgtgtcaa cagttcttca taaagactga gatcataatg gtaaatattc aatttttcaa
    23341 aaaaatctat ctcgttcgga cgaacaccgt aacatgcgat tctcatactt tcttcctcca
    23401 tttcttatga aaataaacct atgccatgaa tcatttgtaa tacaacgatc catacaggtg
    23461 tcagtaccac agcaaaaata gttgacagta aggatgcatt ggaggctaaa agtgcctccc
    23521 tatcaaaact gatagcatag gctgctgcca ctgttgcagc tggtgtagcc atcatgataa
    23581 cgattgttgc cagtgcttca aaattgactg gtagaatatt cgttgccgtc aaaactgcta
    23641 gaatcacgat attcaatgca ggtacaaata ccactttgac tagactgtaa taccatgaag
    23701 ttttttcaga tgcagctttt ttgaagctta cttcacctaa tgttgcgccg attgctagcc
    23761 acgccaatgg tgaacttaaa ccagctaaat aatccatcgg ctgaaatagc cacggtgcag
    23821 tctggtcaat tcgcaaaaac gctacttggc ttatctctcc ttctgcacct gtaactgaca
    23881 cttgtggcag atacccttgg aacagccaaa tgaaaaggcc ggcaaaagta gcgataatga
    23941 ttggattgac gaacatcgct ttgatatttt tagcttccat cttcaagcca ctcattttta
    24001 tgtatccata agaatacaaa aagattcggt agccaatatt gaaaattgag gaatacatca
    24061 cgccgacagg accataaatc gcactgacga taggcgttcc gaaaaaggtc gtagaaccaa
    24121 agatcgtcag gacacgcaac gttgtttgct tatccccttt atagcttaag aaaaaaggtt
    24181 ttgaaactag aataagaata atataaataa ctaatcccca aatcaagaca ttgattcctt
    24241 gcatcagtaa ttctgtattg atatcttgca tgaaagctcg aaatgccagt gctggcaaag
    24301 ccactgttaa gacgactttc gatagaattt ttccaacaga ttcagaaaaa atcccctttt
    24361 ttctacaaac aaaccctaat aatataataa aaactgttga agtgattgca ctgacaatcg
    24421 acatatctgt caaagtcgat cgaaaaatgt caaataagtt catctttctc ctcctaatta
    24481 tgttcattta ttcacaataa aaaaagcttt tattttgcta atacgtatta acatttttaa
    24541 ttttatcttt acattgt
//
LOCUS       DACDSY010000025        23831 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDSY010000025 DACDSY010000000
VERSION     DACDSY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.025"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(55..789)
                     /locus_tag="IWK44_000876"
     CDS             complement(55..789)
                     /locus_tag="IWK44_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9549421.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="IWK44_000877"
     CDS             1116..1526
                     /locus_tag="IWK44_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP9549422.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="IWK44_000878"
     CDS             1519..2220
                     /locus_tag="IWK44_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP9549423.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="IWK44_000879"
     CDS             2220..3806
                     /locus_tag="IWK44_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9549424.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="IWK44_000880"
     CDS             3825..4046
                     /locus_tag="IWK44_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP9549425.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="IWK44_000881"
     CDS             4043..4804
                     /locus_tag="IWK44_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP9549426.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="IWK44_000882"
     CDS             5184..5693
                     /locus_tag="IWK44_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9549427.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="IWK44_000883"
     CDS             complement(5768..6307)
                     /locus_tag="IWK44_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP9549428.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IWK44_000884"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IWK44_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP9549429.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7374..8171
                     /locus_tag="IWK44_000885"
     CDS             7374..8171
                     /locus_tag="IWK44_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP9549430.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8199..8834
                     /locus_tag="IWK44_000886"
     CDS             8199..8834
                     /locus_tag="IWK44_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP9549431.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="IWK44_000887"
     CDS             8854..9627
                     /locus_tag="IWK44_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9549432.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="IWK44_000888"
     CDS             10035..10832
                     /locus_tag="IWK44_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP9549433.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="IWK44_000889"
     CDS             10810..11223
                     /locus_tag="IWK44_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549434.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="IWK44_000890"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="IWK44_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9549435.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="IWK44_000891"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="IWK44_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP9549436.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="IWK44_000892"
     CDS             16000..16560
                     /locus_tag="IWK44_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP9549437.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IWK44_000893"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IWK44_000893"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP9549438.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="IWK44_000894"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="IWK44_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP9549439.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="IWK44_000895"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="IWK44_000895"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP9549440.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="IWK44_000896"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="IWK44_000896"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP9549441.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="IWK44_000897"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="IWK44_000897"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP9549442.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="IWK44_000898"
     CDS             21907..22485
                     /locus_tag="IWK44_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9549443.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="IWK44_000899"
     CDS             22537..23019
                     /locus_tag="IWK44_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP9549444.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="IWK44_000900"
     CDS             23136..23510
                     /locus_tag="IWK44_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549445.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7783 a         4285 c         5114 g         6649 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt t
//
LOCUS       DACDSY010000026        23440 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDSY010000026 DACDSY010000000
VERSION     DACDSY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.026"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(309..731)
                     /locus_tag="IWK44_000901"
     CDS             complement(309..731)
                     /locus_tag="IWK44_000901"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP9549446.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(745..2151)
                     /gene="atpD"
                     /locus_tag="IWK44_000902"
     CDS             complement(745..2151)
                     /gene="atpD"
                     /locus_tag="IWK44_000902"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP9549447.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2174..3076)
                     /locus_tag="IWK44_000903"
     CDS             complement(2174..3076)
                     /locus_tag="IWK44_000903"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP9549448.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3090..4646)
                     /locus_tag="IWK44_000904"
     CDS             complement(3090..4646)
                     /locus_tag="IWK44_000904"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP9549449.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4669..5211)
                     /locus_tag="IWK44_000905"
     CDS             complement(4669..5211)
                     /locus_tag="IWK44_000905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP9549450.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5198..5722)
                     /gene="atpF"
                     /locus_tag="IWK44_000906"
     CDS             complement(5198..5722)
                     /gene="atpF"
                     /locus_tag="IWK44_000906"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP9549451.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5813..6028)
                     /gene="atpE"
                     /locus_tag="IWK44_000907"
     CDS             complement(5813..6028)
                     /gene="atpE"
                     /locus_tag="IWK44_000907"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP9549452.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6081..6800)
                     /gene="atpB"
                     /locus_tag="IWK44_000908"
     CDS             complement(6081..6800)
                     /gene="atpB"
                     /locus_tag="IWK44_000908"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP9549453.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7102..9945)
                     /gene="rnr"
                     /locus_tag="IWK44_000909"
                     /pseudo
     CDS             complement(7102..9945)
                     /gene="rnr"
                     /locus_tag="IWK44_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(9948..10694)
                     /locus_tag="IWK44_000910"
     CDS             complement(9948..10694)
                     /locus_tag="IWK44_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9549454.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10806..11042)
                     /gene="secG"
                     /locus_tag="IWK44_000911"
     CDS             complement(10806..11042)
                     /gene="secG"
                     /locus_tag="IWK44_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP9549455.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11160..11438)
                     /locus_tag="IWK44_000912"
     CDS             complement(11160..11438)
                     /locus_tag="IWK44_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9549456.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11551..12519)
                     /locus_tag="IWK44_000913"
     CDS             complement(11551..12519)
                     /locus_tag="IWK44_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9549457.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12734..12778
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12802..13776
                     /locus_tag="IWK44_000914"
     CDS             12802..13776
                     /locus_tag="IWK44_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP9549458.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13781..14356
                     /locus_tag="IWK44_000915"
     CDS             13781..14356
                     /locus_tag="IWK44_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9549459.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14423..15562)
                     /locus_tag="IWK44_000916"
     CDS             complement(14423..15562)
                     /locus_tag="IWK44_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP9549460.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(15962..17260)
                     /gene="eno"
                     /locus_tag="IWK44_000917"
     CDS             complement(15962..17260)
                     /gene="eno"
                     /locus_tag="IWK44_000917"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP9549461.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(17376..18131)
                     /locus_tag="IWK44_000918"
     CDS             complement(17376..18131)
                     /locus_tag="IWK44_000918"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP9549462.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(18235..19425)
                     /locus_tag="IWK44_000919"
     CDS             complement(18235..19425)
                     /locus_tag="IWK44_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP9549463.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(19535..20536)
                     /gene="gap"
                     /locus_tag="IWK44_000920"
     CDS             complement(19535..20536)
                     /gene="gap"
                     /locus_tag="IWK44_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9549464.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(20577..21614)
                     /locus_tag="IWK44_000921"
     CDS             complement(20577..21614)
                     /locus_tag="IWK44_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP9549465.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            complement(21817..23151)
                     /gene="rpoN"
                     /locus_tag="IWK44_000922"
     CDS             complement(21817..23151)
                     /gene="rpoN"
                     /locus_tag="IWK44_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP9549466.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            23211..23282
                     /locus_tag="IWK44_000923"
     tRNA            23211..23282
                     /locus_tag="IWK44_000923"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23244..23246,aa:Arg,seq:ccg)
BASE COUNT         6295 a         5014 c         4242 g         7889 t
ORIGIN      
        1 agtcttgtcg cagtccctta ttccaaataa acggtgttca atcatctgac cctcggtatt
       61 agctcaaaaa acggcaaaat atgaggaact attttccatt tgtttctcta atactcagga
      121 caaaacgctg atttcacaac ctccgatata cggtgttcca gaagtagctt cttcgaaaaa
      181 taagctgaga tttcacaaaa atttgagaag caattttcgt aaattccttc ttatgtcttg
      241 aagctgacca cttctgtcat aacctctttt tactgttctt aaacacggat aaacatccgc
      301 atctcatttt atgagtgctt agatactttg atacgattga ttgctcggtg caacgcaaca
      361 gttgcccgtt tcaattcgtt cgtatcttgt tttgcttttg cttcttcgat ttggcgttct
      421 gcgcgttgtt ttgcacgttc cgcacgagat acatcgatat cacgttcacg ttccgcacta
      481 tcagcaacga ttgaaacaac attatcacga acttccatga ttccgccatt aacagcgatc
      541 caatcgacgt gggtatctga atctgttcgt tttacacgaa cttcatcaat cgtcaatgga
      601 acgatgattg gtgcatgttt aggcaaaatc ccaagctcac cagctgtcgt acgtgcaacg
      661 acgatagctg catggtgatc atagaccaaa ccggcaggag ttaccacatt tactgttaaa
      721 tattgatcca tggggcaccc ctttctagta gcctagtgtt ttcgcttttt ctacgacgtc
      781 ttcgattcgg ccaacacttc ggaaagcttc ttcaggaaga tcatcgtatt tgccttccaa
      841 gatttcgcgg aatcctttca ctgtttcttc tacagggaca tatgaacctg gaagaccagt
      901 aaattgttca gccacgttaa agttttgtga caagaagaat tggactctgc gggcacgaga
      961 aactaacact ttttcttgat cggataattc atccatacct aagatagcaa tgatatcttg
     1021 taattcacga tatcgttgca atacgtgttg gacttctgtt gctactttat aatgttcttc
     1081 tcccacgatt tcaggtgcta aagcacttga agaagaagcc aatggatcta ccgctggata
     1141 aatcccttgt tctgttaatt tacgttccaa gttggttgtc gcatccaaat gggcgaatgc
     1201 tgttgccggc gccgggtcag tgtagtcatc ggctggcaca tagattgctt ggatcgatgt
     1261 gatcgaacct tttttcgtag atgtgatacg ttcttgcaat tgacccattt ctgttgccaa
     1321 tgttggttga taaccaaccg cagaaggcat ccgtcctaac aaggctgata cttcagatcc
     1381 agcttgagtg aaacggaaaa tgttgtcgat aaacaacagt acatcttgac cttctacatc
     1441 acggaaatat tccgcaatcg tcaaaccagt caaagcaaca cgcatacgtg cacctggtgg
     1501 ttcgttcatt tgtccgaaca ccatggctgt tttttcgata actcctgaat ctttcatttc
     1561 ataatacaag tcattacctt cacgagtacg ttcacctaca ccggtaaata cagaaatacc
     1621 accatgttct tgggcgatat tatggatcag ttcttggatc agtacggttt taccaacacc
     1681 ggcaccaccg aatagtccga ctttcccacc ttttaaatat ggggcaagca aatcgataac
     1741 tttgatccct gtttccaaaa tttctgtact tgtacttaat tcgtcaaagg ctggtgcttt
     1801 tttatgaatt tcgcttcttt ccgcatcttc tgggaaaggt gtttctaaat cgatcgtatc
     1861 tcctagtacg ttgaatacgc gacctaatgt ttctttacct acaggaacag agattgcttt
     1921 gcctgtatca atgacttcca ttcctctttg taaaccatct gttgattcca tcgcaatggt
     1981 ccggatcact ccgtccccta attctaacgc tgcttcaaga acaacttttg atttattttc
     2041 gtcattttta taaacaacta atgcgttgtt gatatctggt aaggattggt ctaaagaaaa
     2101 ttccacgtcg acaacgggac cgattacttg aacaatcttg cctgaactca tcttttttcc
     2161 tccaatctca atactattct agtgccgaag ctccggcaac aatttcggta atttcttggg
     2221 taatagcccc ttgtcttgca cggttgtaag aaaccgtcag ctcatcaatg atattagcag
     2281 cattatcagt tgctgttttc attgctgtca taccagcagc atgttctgct gttttggcgt
     2341 caacgatcgc accgtagatc aaactttcag catactgagg caataactga gacaagattt
     2401 cttcttttga aggttcaaaa atatattctt gttcgaaagt agttgcttct tctggatcta
     2461 agtctgagat tggcagcatt ttttctacac gaaactgact agttagcgaa ttgatatgat
     2521 ggttataaca tacgtataat tcatcgaata cttcgttttg gtacatagtt gttgccatat
     2581 ttacgatttt gcggacttca tcaaagcttg gctgatcaga tagattgcgc agctcatatg
     2641 ctagattgat accgcgtgct ttaaagaagt ctgcaccagt tccgccaatt gcgatcatca
     2701 cgtaatcatc gggtgattgg tggtcttctt ctaaaataga catcgtttgt ttcaatatcg
     2761 agctgttata tccaccgact aaaccgccat ctgcagtgat gacgatataa cccgttttct
     2821 taacaggacg gctgagcaac atgctgttgt aattgatatc ccctctagga ttttctgagg
     2881 caatatcatt taactgagta gcagtcaaat gcgtaacgat ttctcttact tttgttgcat
     2941 aaacctgaaa cttctgtgaa gaagcttcag attttgtcaa cttagaagca gacaccatct
     3001 gcattgcccg cgtgatttgg ctcgtttttt tcgttgaagc gatacgtgtc ttgatttcat
     3061 ttaatgaagc acccattcat ctcacctcac tatgcatttt gaatggattt caatttatct
     3121 tctgtagagg aattatttcc ttttgtagct aagaacatgt ctttatattc ctgaatcgca
     3181 ctgttcaacg cttcttcttc tggaagatct tttgttgtac ggattgtttc aaagatatct
     3241 gaatgattgg tatccagata ttcgaataat tcatgttcga agtctaaaat actgtctaca
     3301 gggatactat ctaagaatcc atgtgtcaaa gcatacaaaa tcactacttg tttttcaaca
     3361 gctagcggtg catgcaattt ttgtttcaag atttctactg tacggcgtcc acgatttagt
     3421 tttgcttgtg ttgctgcatc caagtcagaa ccgaattgtg taaatgcttc taattcacgg
     3481 taacttgcta agtctagacg taaagtccca gcaacttttt tcattgcttt gatttgcgca
     3541 gaacccccta cacgagatac agatagtccg gcatcaaccg ctggacgagt acctgcgtag
     3601 aacaagtcgc tttctaagaa gatttgtcca tcagtgatcg aaataacgtt tgttgggata
     3661 taagcagaga tatctccagc ttgtgtttca acaaatggta aggcagtcat tgaaccgcca
     3721 cctaaatcat cacttaattt tgctgcacgt tccaataagc gtgagtgtaa gtagaaaaca
     3781 tcccctggat aagcttcacg acctggtgga cggcgaagca gcaaggaaag ttcacgataa
     3841 gcaacagctt gttttgaaag atcatcaaaa atgatcaaga catgtttccc gttgtacatg
     3901 aactcttcac ccattgcagt accagcatat ggtgcgatat aaagcaacgg cgctggttga
     3961 gacgcaccgg cattcacaac gattgtgtaa tccattgcgc catatttttt caatgtttca
     4021 acttgtgtac gaactgtaga atctttttgt ccgattgcta catagataca gatcatatct
     4081 tgaccttttt ggttgatgat cgtatcgatc gcaatggaag ttttccctgt tttacggtca
     4141 ccgataacta attcacgttg gccgcgtccg attggtacaa gagcgtcgat tgcttttaga
     4201 cctgtttgca ttggttcgtt aacagattta cgttgcataa cgcctggtgc cattgcttct
     4261 actggacgag ctttgtcagt aacgatttca cctagtccgt caattggctg acctagcgga
     4321 ttaactaccc gtccaatcaa ggcctctcca actggaactt ccatgatctt tcctgttcgt
     4381 tttactttat ctccttcacg aatggactcg aaatcgccaa ggataatgat acctacatca
     4441 tttgattcta agttttgcgc cattccgtat gagccatttg aaaattcaag caactcacca
     4501 ctcatcgcgt tttctaaacc atgtgcacga gcgatcccgt cacctacata agttacggta
     4561 ccgatttctt cgactgaaag cacgttttga taattttcaa tttgttcttt aatcaaggca
     4621 ctaatttctt ctgctttgat ggccattcga ttcacctacc tctttgtact atctatttaa
     4681 ttgattacgc atattttcta attgcgtgcg aatgcttccg tcgattactc gatggttagc
     4741 ttcaacaatc gcgccaccaa taatagaaga atcaacgatt tgttttaatt cgactgattg
     4801 atagttcatt gtttttgcaa cattcttttc taattgtatt cgttgttcgt ctgaaagtgg
     4861 aactgctgtt gtgacacttc ccaacagcaa accttgatgt tcatcatatc gacgttcata
     4921 ttcttcgatc atgaacaaaa gatcatccat tcggttatac gcataaacaa cttctaaaaa
     4981 gtttttaacg attccatcat agccactaac aagcttgtcc atgatttctc gtttttcatg
     5041 aggttctaga cgaacatcac ttaacatatt acctaatcca ggtacttgtt catagatttg
     5101 acgtaagctt acgagatctt gataaatttc ttctgcttta tttgaatcga tcgccagttc
     5161 aaataaagct ttcccgtaac gtcttcctac tgtatattta tctagtttca tttgaagaac
     5221 ctagaccttc gatatattga ttgattaatg attcgtgcat ttctggagat aattctttgt
     5281 tcaagatttt ttccgcgatt tgaagagata gttctgcaac atcgtctttt acagagttca
     5341 gcgctgaatc acgttctaca gtgatatcag cttgggcttt actttttaga cgagctgctt
     5401 cttcttgagc atccttcaaa atattttggc ggcttaattc tccactttct ttcgcatttt
     5461 tgatgatatc agctgcatca gaacgagagg ctaacaattg ttgttcgcgt tcttgttcca
     5521 ttttcgctga gttgatgcga gattgttctg cagaatccaa atcattggcg atcttgtctt
     5581 cacgtttttt caaaatatcg ctgattggtc cccaagcaaa atgcttcaag agagccagta
     5641 gtatcaaaaa tgagccgctg acaacgatga catttcctaa catcgcattg ccaacttctg
     5701 caattgccaa ttgattcagc ataggcgagc cttcctttct ttgaagtact tctagttttc
     5761 atgtaaaaaa gcagtaacaa accaaattgt ttctgcctta atactgattc aattaaacac
     5821 caaaaactag aatcaaggcg acaactaccc ctaagatagg aacagcttcg actagagcta
     5881 ccccgataaa cattgttgta cggaattgac cagacatttc aggttggcga gccattgatt
     5941 cgattgtttt agagataact tgaccgttac cataaccggc tccgattgct gctcccatga
     6001 ttgcgattgc tgctgcgata taattcataa ttgttttcct cctaaattag ataaatagta
     6061 gtttgttttt aaaacgaata ttattcttcc acttcaatct tatggctcat ataaaccata
     6121 gataaagtaa cgaagacaaa tgcttggatg cttccgataa acaaggagaa tgcgatccag
     6181 accatctcta atggaattgc taacggcaat gagaaccagc ctaagcttat taccattttt
     6241 gcaatcaagg ttagcaaaac ttcccccgca taaatattcc cataaagacg taaagcaagt
     6301 gttaataagt ttgtaaattc ttcaatcagt ttcatcggaa acataaaact atatggtttc
     6361 aagaacgaat ttacgaaata tcctttgaag cctaactttt tcaccccaaa taagtgagta
     6421 agtaagatca ttataaatgc caaagttaaa gtaacaaacg ggtctgctgt cgggcttttc
     6481 caaagtgttg tttcgttagg taaaacgatt tttgttacta aaccgatgtt gtttgcaaca
     6541 aaaacaaata aaaacaatgt aaatgccaac aaatggaaat tgctcaattc ttttctaggc
     6601 agattatctg ttacgattcc tcgactaaaa tcaatgaccc attctatcgc attttgtttg
     6661 cctgttggtt tcaacttaag atttcttgta aaaaagtaag ctaagaagaa aacaatcaaa
     6721 caggtcagta acaccattat acaaactgta ccatcaaacc aaattggtcc aatgtggaac
     6781 gttagcgagc gttcatccaa tccatttcac ctcttttccc cgaaattcac ataaaatcaa
     6841 ttcatgtgcg tatctggttt tttccaatcg gtttctaacc acacggtatc ataccaccat
     6901 gaaacagaaa attcaaaaca ttttcatgaa aattagacac ttttttattt cagtcaaaaa
     6961 cgaagcgaat ttttctttat acacggtgct cctgatctaa gaaaaaacct atgataatca
     7021 tgttttatca gattaaatgc taacaaattt tatcattaag taaaagcaaa agcaccgaat
     7081 atgcgaaaaa ggataatgaa attatctaga gaaatttttt aatgttcgat caatttgtcg
     7141 atcgatatct tttcgtttca aatcttcccg tttatcataa gattttttcc ctttcgccag
     7201 accaatcaat accttggcat agccgttacg aatatagact tttaaaggaa caacagtgat
     7261 tccagtattt ttcaattcat tttcaagccg gatgatttgt tttttgtgta ataataactt
     7321 tctcgtgcgc aacggatcat gattaaaaat atttccttgt tcataaggac tgatatgaac
     7381 attatgcaag aaagcttctc cgttgcggac gcgaataaat ccatctttta gattgatccg
     7441 gctgtttcgt atcgacttga tctcggttcc ttgcaagacc atccctgctt ccatcgtgtc
     7501 gatgatcgaa taatcatggc gagctttctt gttttgtgca attaattttc cctcgccttt
     7561 tggcatagct gtttcctcca actcatttta ttttttcttt ttcttctttt taacaccttt
     7621 gtaaaatggc ttcttccctt ttttcttacc gaacgtttgc ttactatcgc tatatcgttt
     7681 gttcttcttg ccatttcttt ctgggcgttt tcctgcttgt tttccttcgc gttttcctga
     7741 ttttccattt cgtgtttttg gccgttccaa cgctgtaacc tcttctgcgg aaacaagttc
     7801 aaaatctact gctcgttcat ctggatcagc tttgactact tttactcgca ctttctgtcc
     7861 gattttaaaa gtcagaccag ttctttcacc tacaagagct aaatgatttt caacaaaatg
     7921 gaagtagtcc tgtttcaatt cattgatatg gatcaatccc tctactgtat tcggcagttc
     7981 gataaacagg ccgaatttca caacagagct gatgatccca tcgaattctt cccccacttt
     8041 gtccatcatg aattctgctt tcttcatgga atctacttca cgttctgttt ctactgcacg
     8101 acgttccatc ttagaactat gatctgcaat ttcaggaagt tccgtagccc acgaactttg
     8161 attgctttca cttttatctt cactgtaaga acggatcaga cgatggacga ttagatcggg
     8221 atagcgtcga ataggagatg taaagtgtgt gtagtaatct gccgctaagc cataatgccc
     8281 aaaattgtct tctgaatagc gtgcttgttg catgcttctt agcaacatag tattgataac
     8341 tgcagcttct ggtttgtctt ctactccttc gatcacatgc tgtaaatctt ttggtgtgat
     8401 cgttcctttc gttcctcgga ccagaatgcc caaagctgaa gcaaaatcaa agaagcgctg
     8461 cattttttct tcttttggct gttcatggat tcggtaaata aacggcagat ttaatttatt
     8521 ataatgttca gctaccgttt cattggccgc aagcatgaaa gattcgatca agcgttcacc
     8581 aattcctctg cttcttagct ggatatcatt tgggtgtcct tcagcatcaa caaggatttt
     8641 tgcttcgcga tcctcaaaag aaatcgcgcc acgtttgatt cgcatcgttt caaggattcg
     8701 atgcaattca cccatttctt ggaacatagg tacgagctga gcatagcgtt gcatcgtttc
     8761 ttcgtcttga tcttctaaga tttgattgac agctgtgtag gtcatccgtt ctgttgtttg
     8821 gatcacactt gggaaaatat catgagagac tacttgtcca tttggatcaa tctccatttc
     8881 gcaactcatg gtcagccttg gaacatgggg attcagtgaa cagataccat tcgataaacg
     8941 ttgcgggatc atcggaatca ctcgatcagt caaatataca cttgtccctc tttcatatgc
     9001 ttctctgtcc aattgactgc cttgtgtcac atagtaagaa acatctgcaa tgtgtactcc
     9061 aagaaaataa ttaccgttat ctaatttttt aaccgtaact gcatcatcta gatcttttgc
     9121 atcttctcca tcaatcgtaa caatgagctg atttcttaaa tctctacgtc cagttatatc
     9181 cttttcgctg atttgatctg gaacttcatc tgcttcagac aatacatcat caggaaattt
     9241 cgtagggata ccattagcca cgacaattga gaggatatcc atgccagggt cattcttatg
     9301 accaatcact tgcttgatta tcccttctaa actagtcgca tacccttttt cagggtaatg
     9361 ggtcagttcg atcagcacaa tacttccgtc aactggacga actccctctg ctgctgcata
     9421 tactttaaat tggttgagct ttctatcttt cgggataaca acgccgtaaa gatccgtttc
     9481 ttgtatttct tcttctgaat aagcaacgaa ttcccctgct aactgagtag tcgcccggtc
     9541 tcgaatctcg actactttcc cctctgctcc gcgatccata tctgtatttg ctcgcttcat
     9601 gatatcgatt gctacggtgt ctccatccat tgcaaaacca gtcgcttctt ttgggatata
     9661 gatgtcatct tcttcgggat caatcgttac aaatccaaat cctcgttcat ttgctcgaaa
     9721 tgttccttcg atcaagattg gttttaaagg aagtttcact tttccttttt tattgaaaac
     9781 tatactctgt tctctttcca tttgagcaat cgtttgtact agtaatttaa aatcatcgct
     9841 tttttggaac cctaaacctt cagcgatttc ttccatagaa aaactgcgtt tgcgactgct
     9901 ttccatgaaa aagagaattt tctcttttat cgtttctttt gtcatattta ttcctcattc
     9961 caaggtaatt tttctaaaaa ttccaagacg tctttttcta attcgcgtct tgctgtacca
    10021 actgtaatca catgaccact ttcaggatac catcgaagtg tcactcgctg acggctcaac
    10081 ttgcgtgcag tttcaaatac gccattaggg tcgatcattt catctttccc agcttgagct
    10141 aaaaatatcg gctgttggat atctgacaac ctactttcta cgattgctgc ctgatcttgt
    10201 atatccatta attgttgttc agctaacgag cggtaagcaa ctgctttttc attgatttct
    10261 ttctctgatt tacctgctgt cttcagcact tgacgtacgt atttttcaaa gttttcaggc
    10321 acgtttgttt ttacaggaga caaaggcgaa cagaaaaaac cgccgccaat cactgattct
    10381 tcttctagtg ctcgtacagc aaagatgccg cccattgata atcctaatac ggcaatatca
    10441 gaaaactgct cagactgtaa aaaatggatg gcttttttag tgtctgccca ccagctttct
    10501 gctttttctt gcaaaatatc atatggctcc attgttccgt gtcctttaaa aagcggcgca
    10561 taaactgtat aatctgcttt ttccaaaaat cgtgcgagca ttcgaacatc gtttggactt
    10621 cctgagtaag cgtgaagcag caaaaccgct cgctttccat gttttgcata aaggggtttt
    10681 ggtaattgtc tcactcgtct cacctctttc gcctcatttt accatagttt ctttcataac
    10741 ttcttcttaa gcgttctctt tgcaataaaa aaagccgccc gccgttttgc gagaggctta
    10801 ttcaactact tcgaagataa atatgctagg acaaataata agatcatcca gatagctccc
    10861 ataactgcag ttgatcgctg catgacagct tcaaagccgc gagctttttg tttgccgaat
    10921 agttgatccg caccgccggt aaatgcactc gctgcactat tttgcttgct tggctgcatc
    10981 ataatcgcaa tgaccataac aatcgataaa acgatcacga ttcccaaaat taaattatac
    11041 atatcgcagc ctccttccca aaagatatcg tctctttaac tttatcatag ataaaagtcc
    11101 gtgacaactc tatataaatg gaataacagc tgaaagttca aaactttcag ctgttattct
    11161 tatgactgac gtaagacgtc ttgatattct ggtttctttt caaactcttt ttttgcataa
    11221 gggcataaag gaatgatctt tttcccgtct cgtcttgcta attctactcc agtatacact
    11281 agcttttcag ctaacttttg accacgatat gctggatcta caaatgtatg gtcaatgatc
    11341 atgatgtctg ggcccgcatc tgaccatgtc atttctccga tctcttgttg ttcatcattg
    11401 aataaagcaa gacgattttt ttcttctttg atttccatat gccgcactcc tcaaataact
    11461 gatatttttt tattcctacc ttattttagc agtctcctga tctataaaca attatttatc
    11521 ctcgaattac ttacaaaaaa gaagagaaaa ttaattcatc gttattcttt gttcaataaa
    11581 tgaaaaaatc actgtccaat aggttttcgg atctttcatc gatgcttcca agtgttctgc
    11641 ttctggaaca agcagcagtg ctttatcccc tgccgtagca ttttgaatcg tgtacgctgc
    11701 ttctgtaggg acaaaatgat ctctttcacc atgaatgacc aataaaggca gatggttgct
    11761 gccaagctgt ctcgtagctg atgcttgctt taaggagtaa ccggcgtatt tctgggcata
    11821 atgattcgca tatctcatga ccattttttt aggaaagaca gtgattttac ttaacatata
    11881 tcggaattct gcataaacag aaacatatcc gctgtctaaa ataaatcctt tgaccgctga
    11941 tggaagtttt tcaccacttg ccatcatgat cgtagaagct cccattgatc cgccgtgtaa
    12001 aataatggac gcattcggct gttcatctaa gatgagttgg atccaggcaa ttaaatctag
    12061 ccgatctagc catcccattc cgatgatttc tccttcactt tctccgtgcg cacgtaaatc
    12121 aggtacaaga acgttgaatc ctttctctgc atatctttta cctatatgag acatgtctct
    12181 tttccctgtg cttcggtaat catgtacgca aatgacccat ttattctgct tatttggatg
    12241 gatgaacatc tgaccagcta gtttcaaccc gtcataactt ttgattgttt tgggtgttcc
    12301 ctgctcccaa aagctttcac actcttcttc taatttttgt cgtttttttt ctactgacgt
    12361 gactgcagca gggtccatca aacgatgacc agtgtcttca taccagtcat atcctctctg
    12421 tactcccgct cttactaatt tctttgcata aaaataagaa agaaaatggt aactgatccc
    12481 tataattatc agaataagaa tgaaataaaa aataaccatt gtacacccgc cgctttctgc
    12541 caaactataa tctaactatt atccaatgta ccgatccata ggattcttta ttttcggcac
    12601 acgtaaattc catttctttt tcattgtatc atttacttaa agttctcctc aatagattta
    12661 cttgtaagag agcatttaca ttcgcaataa aagccagtat actaatttcg tgcattataa
    12721 tcaagacgct cgtcttggac tggttcggaa acttcccaga ataaaaaact aagtatctct
    12781 aaagaaagga gattggaaat aatgcgaaaa gtaattattg attgcgatcc agggatcgac
    12841 gacactttag ctttatcatt agctgtcaaa tcccctgata tcgaagtagt tgctattacc
    12901 gtagtttgtg ggaatgtccc tgcagatata ggtacgcaga atgtgctgaa atgcctggaa
    12961 cgctgtgatc gtttagacat tcctgtttat caaggaatgg aaaaaccttt gaagcaacct
    13021 ttcatcagtg cacaagacac tcatggtatg gatggtttag gggaaacaaa tttcccgatg
    13081 atcttgagaa aacaggcaga acctctccat gccgttgatt ttttggctga ttatttcaaa
    13141 gaaaagacaa atacatcagt cattgctttg ggtcctttga ccaatatcgc ttttgctttg
    13201 aaagtcaacc caaatattgg gaaacatatg gatcgttttg tctcgatggg tggaacttac
    13261 aaaagccatg gtaactgttc gccagttgct gaatacaatt actggtgtga tcctgaagca
    13321 gcttcttatg tattcgagaa tctcaaacaa acgatagaaa tggttgggtt agatgtgaca
    13381 agagaaatcg tcttgacacc gactatcctc gaatactgct gtcaaatgaa tcctgaagag
    13441 ggagagtatc taaaagcgat cactcgtttt tattttgact tccactggaa acaagaaaga
    13501 attttaggat gtgtcatcaa tgatccgtta gctgttgctt attttatcga gggggccatc
    13561 tgtgaaggtt tcaaaagctt cactgctgtg gaaactcaag gaataagtcg tggacaaaca
    13621 ttggttgatc gttatgaatt ttggcagaaa cctgctaaca gtaaaattat gacaaaagta
    13681 gatactcgtc tttttttccg aaagttttta accgttcttt taaatgcaca agaagaaacg
    13741 attatgaaag atttggaaag gttaaagatg ggctaacaaa atgaataaaa acaaaatggc
    13801 aactcgcgta cttacagtga tggctttgtg tattgggatc aattatattg gaggaacgat
    13861 tgctttatgg cttcgtttgc caatctatct cgattctatc ggaacgattt ttgctggtgc
    13921 attgcttggg cctgtacctg gaatgctgac aggattgtcc agcggtattt taagtggagt
    13981 gactacagat attttttctc tgtattattc gccaatccaa attctcacag gcttgcttgc
    14041 tggattgatt ttgccaagat atctcggcaa agataaaaac agcttgaaac tgttattact
    14101 cgctttgatt ttgtcggtac ctggaacgat cgtttcttcg atcattaccg ttcagttatt
    14161 tggcgggatc acctcctcag gatccagtat gatcgttcag ctattacatg ggttaggttt
    14221 aagtcagaca acaagtgtga tcttggtaca ggcaggtaca gactaccttg atcgtttgtt
    14281 gtctatcgtc gttgtttcat tagtcatcac tatgttgcca caacgcttga tatattcaaa
    14341 aagaaaaata ggctgaataa acagagctgg attagcaaaa taatgctaat ctggctctgt
    14401 tttttcattt attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt
    14461 caatcatatc caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa
    14521 cgacaccagg cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg
    14581 catccgttgt tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc
    14641 aatgaatcaa aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca
    14701 attccaaatg atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa
    14761 tgattttatc atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc
    14821 ttgggatgct gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc
    14881 ctaggacagg attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa
    14941 tgaattcatt taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt
    15001 cttttccgtg cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt
    15061 aattagttgg ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga
    15121 gctgttcccc acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc
    15181 cgttaaatgg ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc
    15241 cactcttcat atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa
    15301 agggtgcata ggtccaagac gattcatcac cagccgccac gacatccata tgcccagata
    15361 ggcctaacac ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat
    15421 ctctgtaaga aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg
    15481 ctgctacttc accttcgttt ccattcactg atttaattcg gatgatctct tgtaaaatcg
    15541 cgattttttc ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg
    15601 agtgtgctcg aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt
    15661 tatgaaaaat atgctgctac ttccctccta ttttatttat acgagaggac ttgctcatag
    15721 attcctattc tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt
    15781 tcctttttca aggatgatct tttcttgtac cattctttgt aaacatcata tacagaagct
    15841 agttgcaaaa aaaactctct acctgctgat atttcagcaa gtagagagtt cgattactta
    15901 actaagtgat catactcgac tatctttcaa tagtcgatca tgtatcttca ttagaaaaga
    15961 attatttgtt ttttaagttg tagaaagatt tcaaaccttt gtattctgca acttcaccaa
    16021 gttggtcttc gatacgtagt aattggttgt atttagcaat acggtcagta cgtgatagag
    16081 aaccagtttt gatttggcca gcgtttgttg caacagcgat atcagagatt gttgaatctt
    16141 ctgtttcacc agaacggtga gatacaactg ctgtgtagcc agcttcttta gccatttcga
    16201 tagcttcgaa agtttctgtt aatgtaccga tttggttcac tttgataagg attgagttag
    16261 caattccttt ttcgatacct tcagataatt tttgagtgtt tgttacgaac aagtcgtcac
    16321 caactaattg aactttgtcg cctaatacgt cagttaattt cttgaatccg tcccagtcgt
    16381 tttcgtctaa tccgtcttcg attgagatga ttggatattt agaaactaat tcttcgtaga
    16441 atttgatcat ttcgtcagtt gttttttcgc cttcgcctga atcagctagt acgtaaacac
    16501 ctttttcttt gtcgtagaat tctgaagaag cagcatccat agcaagaaca acgtctttac
    16561 caggtacata gccagctttt tcgattgctt cgatgattac ttcaaaacct tcttcgtttg
    16621 aaccaaggtt tggagcaaat ccaccttcgt caccaacaga agttgctaat ccgcgagctt
    16681 tcaagattga agctaatgcg tggaatactt cagcacccat acgtagagct tctttgaatg
    16741 ttggagcgcc aacaggcatg atcatgaatt cttggaagtc gattgagtta tcagcatgtg
    16801 atccgccgtt gatgatgttc atcattggtg ttggcaatac ttttgtgttg aatccgccta
    16861 ggtagtggta taaaggtact tctaggtagt cagcagctgc acgtgcaaca gcgatagaaa
    16921 cacctaaaat agcgttcgca cctaatttac ctttgttagg agttccgtct aaagcgatca
    16981 tagctttgtc gatagccatt tggtcacgaa catcgtagcc aatgattgct tcagcaatga
    17041 tgttgtttac gttgtcgaca gctttagtta cacctttacc gccataacga gctttgtctc
    17101 cgtcacgtaa ttcaactgct tcgtattcac cagttgaagc acctgaagga accattccgc
    17161 ggccaaaagc tccgctttct gtgtatactt ctacttcgat tgttgggtta ccacgtgagt
    17221 ctaagatctc gcgagcgtaa acatcagtaa taattgacat gttttgtctc tcctttgagt
    17281 ttgttttttc ctaagggatt tccctcatat acgattttag ttaattttgc tcattagtgc
    17341 aaacatttct tgcatttttc gcaaattttc aacaattatt ttacagcgtc taacaatgac
    17401 aagaatgaat ctgcttgcaa gcttgcgcca ccgactaaag caccgtcaac attttctttt
    17461 gccatgtatt cagcaatatt ttctggtttt acagaaccgc cgtattggat acgaactgct
    17521 tctgatactt cttttccata aagtttttca actgtttgac gtacaactcc acaaatttcg
    17581 tcagcgattt gtgcgtcagc tgatttacca gtaccgattg cccagattgg ttcataagca
    17641 ataaccatgt ttgacacttg ttcagctgaa agatcagcta aaccagctgt aatttgtcct
    17701 tcgatccatt cagcagtttt gcctgcttca taggtttcta aagattcgcc acagcatagg
    17761 attggtgtca taccatttgc aaagatagct ttcgcttttt tattgatgtc ttcgtctgtt
    17821 tcatggaaat attcacgacg ttctgaatgt ccgatgatca cataatgaac acctagatca
    17881 gcaagagctg ctggtgaagt ttcaccagtg aacgcacctg agttttccca gtagcagttt
    17941 tgtgctgaga tttttagttc tgtaccttca gctgatgtca ttaattcttg taagaataat
    18001 gcaggagatc cgatgactga atcaactttg tcatttgaag ggattttgtt ttttactgct
    18061 tcagcaaatt cttttgcttc tgatgcagtt ttgttcattt tccagttacc tgcgataata
    18121 ggtttacgca ttgaggaaca cactccttaa tttattgaaa aatggctagg gagtcatgaa
    18181 aactcccttc accttttgat cgattactcg tcagaattga gcaaacttgg tcgtttattt
    18241 atcgttgatt gctgctagtc ctggtaattc tttaccttca agcaattcca agcttgcgcc
    18301 gccacctgtt gagatgtgtg tgaatttgtc agcaaatcct aattgttctg ctgctgcggc
    18361 tgagtctccg ccaccgatga ttgttgttgc gccttctaag ttagcgattg cttcacaaac
    18421 accgattgta cctttagcaa agttactcat ttcaaataca cccataggtc cgttccaaac
    18481 aactgttttt gcaccttgta atgtatcagc aaataatttt actgatgctg ggccgatgtc
    18541 taaagccatc aaaccatcag gaatgtctcc ttcgattact tgtgtttcaa catcatttga
    18601 aaattctggt gcacagacat tgtcgattgg caagacaagt ttgtcgcctg ctttttcgat
    18661 taattctttt gctagttcca ctttatcagc ttctactaga gagttaccga tcttgattcc
    18721 ttttgcttcg tagaatgtgt aagtcattcc gccaccgatc aagattttat ctgctttagg
    18781 aatcaagttt tcgattacgc cgattttatc agaaactttt gcgccaccaa ggattgcaat
    18841 cattggtcgt tttggttctt ctactgcttc accgatgaac ttgatttctt tttccatcaa
    18901 gaatccagca actgttggga taccagttga agcgatacct acgttagaag catgtgcacg
    18961 gtgagctgta ccgaaagcat cgttaacaaa tacatcgcct aatgaagccc agtatttacc
    19021 tagttctgta tcatttttgc tttctttttt accgtcaata tcttcaaatc gtgtattttc
    19081 gaatacgaca acgtcgccgt cattcatgtt gcggattgct tcttctaatt gttctccgcg
    19141 agtttcaggt acgaatgtta cttctttacc taataattcg cctagacgtt ttgcaactgg
    19201 agctaaagat tttccttctt tatcttcttc tgtttttaca cgtccaaggt gagagaaaag
    19261 gattgctttt ccgccttgtt ctagaacgta gttgattgtt ggcaatgctg ctttgatacg
    19321 agtgtcgtca gtgatcacgc cgtctttcaa aggaacgttg aagtcaacac ggacaagaac
    19381 ttttttgtct tttaaatcga tatctttcac tgttttctta gccattttat ttcctcctga
    19441 ataaacttat tatcaaaaaa agcggaagaa gcgcgattgc ttccccgctt ttaaatgtac
    19501 atgtctataa tttacacttg tacgtttgtc tatcttataa gttagcgaag tattctaaag
    19561 tacgtactaa ttgtgcagtg tatgacattt cgttgtcgta ccaagcaaca gttttaacta
    19621 attgtttgtc gccaactgtc attactttag tttgtgtagc atcaaataat gaaccgtaag
    19681 tcataccaac gatatctgaa gaaacgattt cgtctgtgtt gtatccgtaa gattcgtttg
    19741 aagcttcttt cattgctgca ttgatttcgt caacagttac ttctttttca agaactgtta
    19801 ctaattctgt taatgaacct gttggtacag gaacacgttg agcagctccg tctaatttac
    19861 cgttcaattc aggaattact aaaccgatag ctttagcagc acctgttgag ttaggaacga
    19921 tgtttgcagc agcagcgcgt gcacggcgga agtcaccttt aggatgaggt ccgtctagag
    19981 tcatttggtc acctgtgtaa gcgtggatag ttgtcattaa tccttcaaca acaccaaatt
    20041 tgtcgttcaa tgttttagcc ataggagcta aacagttagt agtacatgaa gcacctgaga
    20101 taactgtttc tttaccagtt aatgtttcgt ggtttgtgtt ataaacgatt gttggaacat
    20161 cgtttccgcc aggagcagag ataacaacgc gtttagcacc agctgttaaa tgtttttcag
    20221 ctgctgattt agaagtgaag aaaccagtac attctagaac gatatctacg cctagttcgc
    20281 cccatggtaa ttcttctggg ttgcggttag ctaatacttt aacttctttt ccgtttacgt
    20341 tgaatgaacc ttcatgaact tcaactgttc cgttgaaacg tccttgagtt gtgtcgtatt
    20401 ttaacaagtg tgccaacatt ttagcatctg tcaagtcgtt gattgctact acttcgattc
    20461 catctacatc ttggatacga cggaatgcta gacgtccaat acgtccaaaa ccattaatac
    20521 ctactttaac tgtcatttaa gatttcctcc ttatgaaaat caaaaaaata tttattttaa
    20581 agggttaccc cttttaaaat ctcatttgca gcagcttcat cagtgatgag ccacgtttgt
    20641 tttggtgcat ttttcatata agctcgaatt gctttggctt ttgattttcc gcccgcgatt
    20701 gccatgatga tcgggacttt ttccaaatct tgcaattcta tgccgattct agggacctta
    20761 taaacgactt cacctttttc attgaaaaag tagccaaaag attccgcaac agcatttgct
    20821 tgtttcagca tgactaattc tttttcagtc atcttacgac gtgctgccat gtgtaatgca
    20881 cgcccaatac tatgaataac acaattcgct tcactaatca agtttaagac ttcttggata
    20941 gatggctcat tgagtaaaga attgtaagtt gccagactta attgttccgg aacataaagt
    21001 gcacggtggt taccgtttgc tttagcagcc attcttgcac tgacagaatt tgcctgtaca
    21061 gttatcgctt caccaatccc gcctcttgca gggacaaaca ggttatgccg tttcttattt
    21121 tccagattag acaattgttc ggctaccaca gccatagtag ttcctcccat cacagcgatg
    21181 atattctcgc cttctggaag tagtcggtct agggcttcgc tcagcgcttc gccgaatgca
    21241 tcagccactt tggtctgctc ttcgctgttt cctgaaacaa cgatacagcg ttggttacca
    21301 aaatattctg ccaactgttg ttcggtttga tgcgtaccta aaagctgatc catgaagttt
    21361 tcaaggcttt gataaacttc ttcgccctta gttgtcagcg tcataccgct ttttgaggtg
    21421 tcgatcaaat tcagattttt caaaagatct gtttctgttc gcaatactcg ttctgtgatc
    21481 cccatacttt ccgataaact tcgacgtcca atcggctgca tccaatagat atttcgtaaa
    21541 attttatagc gttcttgcaa cacttcgata atatctggtg caacagattc aatcattttt
    21601 atttcatcca gcatagcgat ctccttagtt ggacacttta cgtccaatgt agacttaaaa
    21661 cgacccatat acgataaaaa aagaaattaa ctattcgaat ttcacatcaa taaagggaaa
    21721 tctcaacctc acgactgtaa gtctaacagt tttgctctcg tttttcaact aaaaagacta
    21781 gaaaaagtga aatacttttt ctagtctttt gactgattag ttatcataac gttttctttt
    21841 tgatgaactc gggattccta ataaatcacg atattttgct actgttcgtc ttgagatcgc
    21901 tatctcatct tttttcagca attcaaccaa tttctggtca gacaacggct tgtttttgtc
    21961 ttcttgatcg actaattcct gcagcttttt cttcgctgta tctgctgaaa ggtcttctgt
    22021 ctgttctgta ctattcgtag gaattcgtgt agtgaaaaat ttttttaatt caaatacacc
    22081 gaagtctgtt tccaaatatt tcccgttgac tgcacgactg accgtggatt catgcacatc
    22141 tatctcagtt gcaacttcct ttaacgtaag aggcttgatt ggacgttcct tgtcgataaa
    22201 gaaagctttt tgccgggaaa caatgacttg agcaacccgc aaaattgtat ccccacgttg
    22261 taggatcgtt ttcttcaacc attcgaattc ctgtttccgt tcttttaaat aattttcagt
    22321 ttctttatct gcttgttgtg acatttgttt gaagtaactc tcttgaaagc gtatatttgg
    22381 ctgatttttt cggtttgaga taacttgtac atttttatcg tcgtccacta atacccggac
    22441 atcaggaata atgtacaatt cgctgctacg gtcgaaaatc cggccagggc ttggactcaa
    22501 cgtttgtaaa tagtcgaata tctgctgaac agcatgtaga tcaacagaaa aacgttgtgc
    22561 gatttctttc cattttcgct ccaccagttc ctcaaaacat tcttccaaca cgatataagc
    22621 taattccggc gcataatcat ctcgttctgt ttgaagcatg agacattctt gcaaactacg
    22681 tgcacctact ccagctggtt ctaattgctg aatcagagtc aacgcatcga gcatttggat
    22741 aggcgtagct ccaatctctt ttccggcttc ttctaaacta atcgtcaaaa atccgttcaa
    22801 atcgatgtat tctacaagat aaagaacgat tttcctcaaa aaagtatctc ggtaattcaa
    22861 atggacttgt tcgatcaatg aatcaaataa agaccctttt gtatcaggaa tctgatttag
    22921 gtaatttgtc tcttctcctt cataagaaga tgaagaagct tttatgtaat ctggctgcca
    22981 ttccggctca acaacttcca ccaatggatt ttccattgct tgattctcaa cgaatgccaa
    23041 cagctcctca ctgttatatt gtaaaacttg gatggactgt tgcaactgtt gcgtcatggc
    23101 tagtttctgt gtctgctgct gtttttgtga aaaattttgc tcaaatttca tttttttgtc
    23161 caatcccctt gtaatttttt atctgtttag ttacaattat cccagtgctt gcgtccttag
    23221 tgtagtggat atcacgtaag attccggttc ttgagacggg ggttcgattc cctcaggacg
    23281 cgttctactg ctattttagc ataatttacg aatagttgct tctttatttt taagtgaaaa
    23341 ctgtccgcca aagaaaattg gtggacagtt ttttgttgta cctgtataga tattgttaag
    23401 cctttgcaag taaggtagtt atgtgttcaa ctacttatta
//
LOCUS       DACDSY010000027        23036 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDSY010000027 DACDSY010000000
VERSION     DACDSY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.027"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            476..727
                     /locus_tag="IWK44_000924"
     CDS             476..727
                     /locus_tag="IWK44_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549467.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            761..1039
                     /locus_tag="IWK44_000925"
     CDS             761..1039
                     /locus_tag="IWK44_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549468.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1636..1893
                     /locus_tag="IWK44_000926"
     CDS             1636..1893
                     /locus_tag="IWK44_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549469.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2006..2617
                     /locus_tag="IWK44_000927"
     CDS             2006..2617
                     /locus_tag="IWK44_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549470.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2796..3353
                     /locus_tag="IWK44_000928"
     CDS             2796..3353
                     /locus_tag="IWK44_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549471.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4052..4573)
                     /locus_tag="IWK44_000929"
     CDS             complement(4052..4573)
                     /locus_tag="IWK44_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9549472.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4772..8464
                     /gene="nifJ"
                     /locus_tag="IWK44_000930"
     CDS             4772..8464
                     /gene="nifJ"
                     /locus_tag="IWK44_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP9549473.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            8653..9540
                     /locus_tag="IWK44_000931"
     CDS             8653..9540
                     /locus_tag="IWK44_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP9549474.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            9537..10706
                     /locus_tag="IWK44_000932"
     CDS             9537..10706
                     /locus_tag="IWK44_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549475.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            10730..12085
                     /locus_tag="IWK44_000933"
     CDS             10730..12085
                     /locus_tag="IWK44_000933"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP9549476.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(12157..13962)
                     /gene="glmS"
                     /locus_tag="IWK44_000934"
     CDS             complement(12157..13962)
                     /gene="glmS"
                     /locus_tag="IWK44_000934"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP9549477.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            14371..14661
                     /locus_tag="IWK44_000935"
     CDS             14371..14661
                     /locus_tag="IWK44_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549478.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            14674..15699
                     /gene="sppA"
                     /locus_tag="IWK44_000936"
     CDS             14674..15699
                     /gene="sppA"
                     /locus_tag="IWK44_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9549479.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            15702..16361
                     /locus_tag="IWK44_000937"
     CDS             15702..16361
                     /locus_tag="IWK44_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9549480.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            16518..17450
                     /locus_tag="IWK44_000938"
     CDS             16518..17450
                     /locus_tag="IWK44_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549481.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            17604..18893
                     /locus_tag="IWK44_000939"
     CDS             17604..18893
                     /locus_tag="IWK44_000939"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP9549482.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            19140..20390
                     /gene="clpX"
                     /locus_tag="IWK44_000940"
     CDS             19140..20390
                     /gene="clpX"
                     /locus_tag="IWK44_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP9549483.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            20473..21060
                     /locus_tag="IWK44_000941"
     CDS             20473..21060
                     /locus_tag="IWK44_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP9549484.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            21425..22753
                     /locus_tag="IWK44_000942"
     CDS             21425..22753
                     /locus_tag="IWK44_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549485.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            22764..22973
                     /locus_tag="IWK44_000943"
     CDS             22764..22973
                     /locus_tag="IWK44_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549486.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT         7660 a         3920 c         4914 g         6542 t
ORIGIN      
        1 atacaaataa ttggtttatt ctggcaggcg taatgctttt ttctagtttc tatttattta
       61 gtaatgctct attagcacag cgagggaaat ataagtttaa aatattagat tagatattat
      121 ttttagaggg gataaatata aaatgaaaag aggttattta taatggaaaa agtattacca
      181 attaagagct actgtattcc aaggcttttc atcatagaat ttcaaaagta aagacatgtg
      241 tttcacaaat agtgtctatc ctggctaaat aattcttcca ctatctgctt taatttcagt
      301 agacttttcg gcaagttatt tgctaatgag atgaatagag attacaactc gcctataacc
      361 gcttaaaaca agctttttta aagtatagtt agcaagtcgt attcatatgt tcaaagagga
      421 gggcttatga actttgaatt cattccgtgt gaacagaaca gtagggggaa gtaaaatgaa
      481 agaaaagcct ataaataaat ggtcattgta taaaaattca atgtttggag ttgttatctt
      541 tggtgtttgt gctggattat tccttttcat attattacaa acggtgactc ttttctcttc
      601 taatcctact tatggatgga gagacagtat agccttaggg atgtttgcgg gaggatatgg
      661 attcttattg ttgttctcaa ttctcacact aagtccaatc catggactca ttcaactttg
      721 gaagtaagaa cgagccttaa acttttcgtt ctcggaagaa atggaaaaac acaacataac
      781 aggcagtatg tatgtggatg atcaatggtt tatcagcact gatgaggtaa aaatagctgc
      841 cattaggaga gattacctgc agaaaattca taaaataaaa gatcctaatg ggaaaaaggc
      901 aaggttaatt ataactttga taacatctaa tggtaagaag ttaaaacttg caggatcata
      961 tgataatgtt aaaaagttcg aacattggta tctgcaaaaa gacagcatta gtcttaataa
     1021 acaatttgaa aaaaattaat caaagtcatc ggcaacacga ataatttgac atcttgatat
     1081 tgtgatataa gcaaaaacgg catttctgag accgaacagc ataaaaaatt taaatgttgg
     1141 aatttatata agcaaaaccc aagaagctag atgcgataga gcatttaatt tcttggggtt
     1201 ttgcttggga atcagcatat caattaaaat gatcatctcg gtatttcaaa agtgggtacg
     1261 attacaaata gttggagcaa aatagtgggt aaaattacca ttccagtgtc tgtcttgcct
     1321 ctttttcgtc aatcacaaat agacgagatt cataggaaaa atcgatacag ggtatgattt
     1381 caatgatatt atgaccaatt acaaaataga ttgataaagc attttttgca tttttataat
     1441 agtttattga taataatgta tgagatacag tataatgatt taaaaggtaa cgtgttgtga
     1501 ccgaattaaa tgaattaatg cctatttttt atattcctta gtggacaaaa atggagacag
     1561 tgaagttagg agcattgtat cctttgttgc gtagttcaaa aaagtaagcg atttctataa
     1621 tggtcagggt aaacggtgag ggctgtccac gtaaatccta ctattgaaat cacaatgatc
     1681 ggacattcaa aaggtggtgc tgaagcagca aattcagtag ctataaatac gaatgcgatt
     1741 atatttaatc cagcgacagt aaatttatct gcttatgaat tagactcaaa cacatataca
     1801 gcaaacatgg tagcttatgt tgtcgaggga gatgcattaa atgttgtttt tggaggagta
     1861 tacacctatt ggagaattaa tcatgttaga tagacaatat gatgcacctt agtggagaat
     1921 gatatctcgc ataggtaact cagtaaaaaa tcaaggtatg gaagcaatta tctctgcatt
     1981 ggaagatcga tatagatagg cggatatgaa tagtaatagg gggaataaaa tggaatacaa
     2041 tggatcaatg agaattgtac aaattatatt ttggattaca tcgggaattg tgatcatagg
     2101 aggagttttt ttgatgttgc caagcttaat atttcctttc tttgcattaa tctcacctaa
     2161 aatacccgaa ccggaaatca cctatggaga atttccattt aggattgaat acgaactaga
     2221 tggacagatt aacataattg aagatactgt catagctgag tttaacgggt gtgagttcag
     2281 cgcaggttct atgaaaagag aacgaagatg gagaagtaga ttagctagtg gtagagaaga
     2341 tctacctttt ggggacgatc taggaatcta ttttagtcga ggaagtgcgc aatattatat
     2401 gggggaaaat gtacaatcaa tgtcattaaa accacatata gcccttagaa atctcgagga
     2461 aggttttaaa agagaggtag actttatatt aggagaaagt ggatacataa gaactgtact
     2521 taatttcgga gaagcgcaag aagttttgac acaatacgga attaccttaa tcaactggga
     2581 aatatctgaa ccgattgtta acaattttgg tgattagaaa acaattacga taaatagaaa
     2641 caagccaaat catcagcaag taaaagctga tgatttggcc tttcttatac gagtagtagg
     2701 aattcatagc gaatattatt acgaccagta gatattttga tattaaagga gtattacgaa
     2761 ctaatatgtg agaatgtaga ggtgatgtgc gaataatgag aaatttatca aatagaaaca
     2821 aaatattaat cattattgtt gttatagctg tgtttcatct gggaacgaat gcggttttaa
     2881 gtagaataat attgggtcct aagccaccaa gaccagaaat tacccgaggg gagtttgatt
     2941 ttagattaga atatgaagta gatggggaaa ggattgtgat agaagataca attgttgcat
     3001 tatttgatgg attttctgca gatgcaggta gcatggcatg gtatcgcacg tggcgtctac
     3061 atttagcaag tgatagacga tcacgaaata tcttattaga tgaattagag gatggtcgaa
     3121 gaatatatta tgtcccagaa agtgctaact attctatggg agatgtacag aaaaaaagag
     3181 agcctaatcc taattggtat ccatttaatg gcgtaacaat agaatatcca cgcaataaaa
     3241 cacctgaaat aggtgcaaaa tttataagtg gtttagaaga tctgtacgat aggtttggaa
     3301 tccgtcttat tagttgggaa cacgatccac caatagaaaa tcgctttgaa taatcgaaat
     3361 ctactaatcc atttcatcag ccagtgaatg ttgtaatgga acaatttggt ttagtgttca
     3421 aaaccattga aatactaggc tgtgatcagc aaaaatgaat ctgaataaaa aagagagcaa
     3481 ctattagctg agaggagtta atagttgtta gctttatacg aatcaaagtg gcaaatacag
     3541 atgcgtatgc atttgtgttt gccatcttgc gtttggtaga cgagataggt ataagctaaa
     3601 gaaacaactt gtttaaaata aacgacctag ctcaacaaat gaccatctaa tcaactaaca
     3661 gcatacataa aagcaactat ttttgaagta gaattgaaat cgggataact taaaaagtga
     3721 ttgggatact agtgagaatc accgtacata aatattactc gttaagggaa attgagaaat
     3781 tttgtatgaa aatttaagaa ttagttagta tgcgcaagtc aaaaatatga ataaattatt
     3841 tggtggagat aatttgctta gcttgtcata agaaatgaga taatctggca gatgcaagct
     3901 tttggacacc atttattcgg aataaggagg ctgggacaga agcgtttaac tccaagaaat
     3961 aagaaggagt tgcttctgct tccgccgttt atacgtgttt agagcgtgag acaaaagtgt
     4021 tctttacttt tgtcccacgc tccttatttt tttattttgt ttggatcaat tcttttaatt
     4081 cctctattga aatagattca tcaaagattt ctttacccaa gatgacagtt ggaacaaatc
     4141 gaatatgtgc ttgctcagct tcgttgatga cagcttggct cagctgttcg tctttttgtt
     4201 ctgtcagctt tagtttatct actgcaaaat ccgctacttc ttggagagaa agatgtttcc
     4261 actcgtcttg agtatcgaat atttgtttga tctctttgat cgctttttgg ccatcgctga
     4321 ttgtgagata ccggtgcatc acgttccctc gctgtaagct ttctttttct ttatcgaaca
     4381 atttgatcac tcgctgcaat tgcccgcttt ggactgcttc ttctaatagc tcataagatt
     4441 cttcaaacca ttgtcggcaa tacggacaag ctaagttgat gaattcgacc atttttttag
     4501 gagcattcga tgaaccataa aggattcctt tttgtgtatt tgttttcgtt gcatcaatca
     4561 ctgatatatc catgttaaaa cacctctttc gcttatagtt tatacatgtc tgacaaaaaa
     4621 aggcaacgat ttagactgtt tgtctaaaaa ttagatgacc aattcaaata gatacatgta
     4681 aaatattttt ataaataaaa gaaaaaaatg gcatgtttta cgttataatg gaacagataa
     4741 taagagagaa tatgaacaag gatgatgaat catgcgaaaa atgaaaacaa tggatggcaa
     4801 tacggcagcc gcatatattt cttatgcatt taccgagatt gctgcaatat atccaataac
     4861 acccagttcg accatggctg aattagtaga tcaatggtca gcagcaggaa aagaaaacgt
     4921 attcggtcaa ccagtaaaag tagtagaaat gcaatccgaa gcaggagcag ctggtgtggt
     4981 ccatggatca ttgaaaactg gtgcactgac aactacatat accgcttcac aagggctgct
     5041 tttgatgata ccgaatatgt acaagattgc tggggaacta ctgccaagtg ttttccacgt
     5101 tgctagtcgg gcagtgacga caaatgcgtt gaatattttc ggagatcacg gagacgtgat
     5161 ggctgcaaga caaacaggat ttgctatgtt agctgaaagc agtgtacaag aagtgatgga
     5221 tttagcacct gttgcccatc ttgcttctat tgaaggagaa gtcccttttt tgaatttctt
     5281 tgatggattc cgtacgagtc acgagattca aaaaatcgaa gttttggaat atgaagaatt
     5341 agctcctctg atcaatcagg agaaattagc ttcatttcgt aaacgaggga tgaatcccaa
     5401 tcatccgact gttagtggta cgaaccagaa tccagacatc cattttcaac aacgggaaac
     5461 gatcaacccc tattatgata aattacctga aatcgttcaa aaatatatga aagagatcaa
     5521 ccgattacga ggaacttctt atgacttggt gacttattac ggagcagacg atgcggaaga
     5581 agtcattgtt tctatgggat cagttgcaca gacgattgaa caaacgattg attacttgaa
     5641 tgcaaacgga cggaaaacag gttttttgaa tatccatctt tatcgtcctt ttccagtgga
     5701 aacattcgtg caaaagttgc ctcatacagt aaaaacaatt gctgttcttg atcgaagtaa
     5761 agaaccaggg gcaggtggag aacctctctt attagacgtt caaagtgcgt tatatgattc
     5821 tgaacttcgc ccggctgtta ttggtggtcg ctacggattg gggtctaaag atgtgactcc
     5881 cgaccaaatc tcagcagttt ttgatgaatt gaaaaaagac ccaagcatac gcaaaaaacg
     5941 gtttaccata gggatcgtgg atgatgtgac ttatcaatct ttggaaccaa aggaaagtct
     6001 ggatttgaca gaaccgcaaa cattccaggc caagttttgg ggatttggat ctgacggaac
     6061 agttggagca aataaatcag ctatcaagat tattggagat cacaccgata aatatgcgca
     6121 aggctatttt tattatgatt cgaaaaaatc tggcggtctg actgtctctc atcttcgttt
     6181 tggagatacg ccgatacgtt cagcgtatct tgtcgagcat gcggaccttg ttgcctgtca
     6241 cacaccagct tacctgcata gctatgactt ggtcaaagga ttgaagccag gtggcatttt
     6301 tttattgaat actctttgga gcgacgaaca attagaaacg catctgccct taaaattaaa
     6361 acgttacttg gctgaaaata acattagatt ctatacgatc aatgccatga gattggccca
     6421 agaagtcggt ttgggacgcc ggatcaatac ggcaatggaa acggcttttt ttaaattagc
     6481 tgacatcatt ccttttgatg aggtgcttcc gctgctaaaa gaagaagccc tgaaaagcta
     6541 tggacataaa tcaatggcta tcgtagaaaa aaacatacaa gcaatcgatc ggacgaccga
     6601 actgcttcat caagtcgcga tcccagacca ttggagaacg ttggagatgc ctgaaaaaat
     6661 caataaaagg aacactggtt atatacaaga aatacttgaa ccagtgaatg cacaggaagg
     6721 aaatcaactc tctgttggta cacttgtagc aaatggaatg acaactggcg aaatgccttt
     6781 aggaacggct tctatggaaa agcggggcgt cgctttagaa gtacctgagt ggattagtga
     6841 tcgctgtacg atgtgtaatg aatgtgcctt tgtttgtcct catgctgcca tccgtccatt
     6901 tttagcagat gaagaggaaa tggaagaagc gccagaagga tttatcgtga gagaaatgcg
     6961 aggcgcagac ggcgtaaaat accgtatcca agtttcagtg gaagactgta caggttgtgg
     7021 tctgtgtgta gaggcttgtc ccgcaaaaga aaaagcttta gtgatgaagc cttatgaaga
     7081 acagaaagaa caagctgtga attgggcttt tgcaatgaca ttaagacaaa aagcaaatcc
     7141 agtcaaagga aagaatactg tcttgtctac acaattcaat caaccgcttc ttgaattctc
     7201 gggcgcttgc tcaggctgtg gcgaaacacc ttatgtaaaa ttattgacac aaatgttcgg
     7261 tgatcgaatg atgatcgcga atgcgacagg ctgttcttct atttggggcg gtgcttctcc
     7321 tgcttctcct tatacgacca atgaatgtgg tcaagggcct gcatggtcaa attctttatt
     7381 ggaagataat gctgaatttg gttatggtat gcttctagcg gcacagactc gtcgcgaggc
     7441 tttggcaagt tcaatcaaag aagtattgcc aaaagtatca gaagaactac gagaattgat
     7501 ggaagactgg ttagtacaca tgtttgagag tgaaggaacg cagcaaagag cagcaaaact
     7561 gcaagctgcg atcgaaagcg aacgatcaat tttccctgaa ctggaggata tatacaagag
     7621 aaaggatcta ttcgtaaaaa acagccaatg gatgattggt ggagacggat gggcttacga
     7681 cattggtttt ggcgggatcg atcatgtact tgccagcgga gcagacgtga atatccttgt
     7741 tttggataac gaagtctatt ccaatactgg cggtcaaacc tcaaaagcaa caccagcttc
     7801 ggcaatcgct aaattttcgg ctagtggaaa gtatgcttca aagaaagatc taggaatgat
     7861 ggcgatgaca tatggaaatg tttatgtggc gcaaattgct tcaggtgcca atcaaatgca
     7921 aacgatcaag gcatttgaag aagcagaaaa gtatcctggt ccttctctga ttattgccta
     7981 tacgccttgt atcacacatg gcttgcttgg cggaatgaaa gaatcgctta aagaagcaaa
     8041 agaagctgtt tcttctggtt attggtcatt atatcgatat aaccctacaa tggtcgaaaa
     8101 aaacaagaac ccaatgactt tggactacaa aaaacctgat ttctctgcta tgcctgattt
     8161 catgaggaaa caagttcgct tttcctcgct agataacgca aatccaaatc ttgcaaaagc
     8221 tttatttgaa aagactgtcc acgacgctaa aacacgtttt tacaactatg ctgcactgac
     8281 gggagaatta gaaaaaatca agaaacgact tgaaccagag gaatctatcg atgtacccgt
     8341 taagactaga aaagaaaaaa cgacggacct tgaagcagaa gaaagaagag ctgctagaag
     8401 agctgccaga gcagaacgac gtaaaaaaat ggaacaagct acaaaagaaa aaaaggaaaa
     8461 ataacagaaa aaatataatg aataattctc tgaagtttaa aaatatttat tgcagagaaa
     8521 aataaaacgc atacttgcaa ttttcttgaa aattatgcgt ttttctcaag aaaaatacga
     8581 gcatatcagc ctctaaaatt aaaacatatg atatactaga gaggaaatct caaaagagaa
     8641 gagaggcttt ttatgtcatt tcaaatcggg gaacttttta atttggacta ttggcagcaa
     8701 atgttttccg gtgacttgtt ctctctgaat tttttggtga acatacttga tattctggtt
     8761 gtctggtatc ttgtatacaa actgatccaa ctgctgcgag gcacaaaagc gattcagctt
     8821 ctgaaaggtg tagctatttt tattgttatc cgaatcgtag cggaaatcat tggtctgcac
     8881 accttgtctt ggctgatgaa ccaagtcatt acgtacgggg tgatagctgc tatcgtgatt
     8941 tttcagccgg aaatccggag aggactggaa catcttggac gcagttcgct tttcaaacaa
     9001 tcaaaaagcg aacagcagga agatgagaag atgatcttgt cttttgacaa agccatccaa
     9061 tatatgtcca agcgaaaaat cggtgcgttg atcactatcg aacgtcatac tggattagat
     9121 gagtatattg agacagggat tgctttagat gcagatatca ctggggagct gctgatcaat
     9181 atctttattc ctaatacacc gctgcatgac ggtgcagtta ttgtaaaaga aggaaagatc
     9241 gcagtagcaa gcgcctatct tcctctatca gaaagtatgt tgatccctaa agaattcggg
     9301 actcgacacc gggcagctgt cggtataagc gaagtcagtg acgcgattac catcgttgtc
     9361 tctgaagaaa ccggggacgt cagcatcacg ttagataatg aactgatggc tggcttatcc
     9421 cagcaagaat atttggcaat cttgcgtaga gaactaattg ctgaagagaa aacaaaaaac
     9481 aagaaaaacg tgattcaatc tttcctagac ggtatgacta agggagggga tcgcaaatga
     9541 tctcaaaaga gaaacggaca agcattcttt acagttttat tgcattgctg ttcagtattg
     9601 cgctgtactt caatgcaaat ggccagtccg ttccaaatac gctttctgga aatgaagcgt
     9661 ataatcaaac agttacgggg gtgccgatca agatttctta tgattcaaag cgctactata
     9721 tccatggata tgaaaatgca gtgactgtca aactttccag tgcaaaccga gttcagttga
     9781 atgcagaggc aaacgaagat acacggatgt tccgcgtcac agcagatatc actaagctgg
     9841 gcgaaggaac acatgaagtt ccgctaaaaa ttcaaaattt gagcagtgct gtcacagcaa
     9901 agatcgaacc atcgactatt acagtaacgg ttgagaaaaa ggtaataaaa gatttcgatg
     9961 tggaaacgtt gattccttca acgttcacac catctggata tgaacttgat gatacttccg
    10021 ttagtcctaa aaaagtctct atcactacag gtgacaaaac attggcagaa atcaaacgag
    10081 tagtagctga tgtggacgct tcgatggtca cagatgatgg gattaatagt gaggtcccaa
    10141 tacaggcgct gaatgcagca ggggaagtat taagtatcgt ctctgatcca gtgcaagtaa
    10201 cagtgagagc tgattcgatc aagccaagca agtcggtacg actttatggg atacagcaag
    10261 gaacaccagc tgccggggta gaaagctatg atttcagttt ttcaaaactg gaagcagaag
    10321 taagcggctc tagcgatttg cttgcatcga tcggtgacag tattgctgta ccgatcaatg
    10381 tctcaggtat caatcatcga acaactcgta aaattgaaat accagtagaa gaggggttaa
    10441 gtatcaagcc cgaatcagtt agtgtggaga ttacacccgt gttgcaaacg tcaagcagta
    10501 gctctgcttc gacgagcagt cggacacaag aaaatacaag tacgagtaga agtatttcat
    10561 ctgctgattc aggaacggaa ccatcgacaa caaccatttc ttccagttcg gaatctggga
    10621 caagcagctc gaccacaagt acagaagaaa catcagaaac aagcgaatca acacaaatga
    10681 gcagaacaaa ttcctcagaa aattaagtta aaggagtaat tttacaacta tgggtaaata
    10741 ttttggaaca gacggcgtac gcggtgaagc caacaaggaa cttacgccag aacttgcatt
    10801 taagctggga cggtgcggag gttatgtcct aagccagcat gaagaagaag gaaaacgccc
    10861 acgcgtgtta gtagggcggg atacaagaat ctcaggtcaa atgttagaag aagcgctgat
    10921 ttcaggactt ttatcggtgg gtatcgaagt gttccagcta ggtgtgatct ctacaccagg
    10981 tgttgcttat ctgacacgtt tgcaaaaagc aagcgcaggg gtcatgattt ctgcttctca
    11041 taatccagca caagataatg gaatcaaatt tttcggtgca gatggtttta aactagtaga
    11101 tgaccaagag ctagaaattg aagcattatt agatgctgaa gtggatgaat tgcctcgtcc
    11161 atcagctgaa ggattgggaa ctgtagaaga atttccagaa ggcttattga aatattctca
    11221 attccttgta caaacgatca acggtgacct ttccggtttg accatttgtg tcgatgcagc
    11281 aaacggagca acttcaacaa gtgttaatcg tttgtttgcc gatcttgaaa cagattttta
    11341 tacaatgggc acaaatccag acgggttaaa tatcaatgac ggcgtaggct ctacccatcc
    11401 agaacgttta gctgagatgg tcgttgaaaa aggagcagat gctggactag ctttcgatgg
    11461 cgatggcgat cgtattattg cggtggatga acttggaaac attgtcgacg gtgataaaat
    11521 catgtttatc tgtgctaaat acttggcaga aaaaaatcga ttgaaaaaag atacgatcgt
    11581 tacgactgtc atgagtaact taggattcca caaagcggtg gaaggaatcg gtttgaaaga
    11641 tgtcgttaca caagttggtg atcgttacgt tgtagaagaa atgcgcaaaa acgattataa
    11701 ctttggcggc gagcagtctg ggcacatgat tttcttagat tttaacacaa ctggagatgg
    11761 gatgctttca ggaattcagt tactgaatat catgaaacag acaggtaaga aattatcaga
    11821 gcttgcagca gaggtaacaa tctatccgca aaaattagtg aatatccgcg taagtgataa
    11881 aaacggtgcg atggatgttc cggcaatcaa aaaagtgatt gaagaggcag aagctgaaat
    11941 gggatcagaa ggaagaatcc tagttcgtcc ttccggtaca gaaccattac ttcgagtgat
    12001 ggcagaagcg cctactgatg agaaagttaa ttactatgtc gataaaatcg cggcagtagt
    12061 caaagaagaa atcggactgg attaagagat attttcataa aaaataaaag aacaaggacc
    12121 agtgtcagat gtctgacact ggtccttgtt cttttattat tctactgtca ctgatttcgc
    12181 taaattacgt ggtttgtcaa cgtcgaatcc ccgtaatagc gttgcgtaat atgcaagtaa
    12241 ttgagtcggt acgactgtgg ttagagtagt tagataaggg tggacagttg gtagcaccag
    12301 ctggtcactt tccttttcta atccctctga gacaataact agtgttttag cgccacggct
    12361 ttcgacttct ttcaggtttc ctcgagtatg cgcaccagtc acttcttcac tgatgattcc
    12421 gatcactggt gttccttctt cgatcaaagc aatcgttcca tgtttcaatt cgccagcggc
    12481 aaaaccttct gcctggatat aactgatttc tttcaatttc aaagcagctt caagagaaac
    12541 gttgtagtca gcaccacgtc caatgtaaaa ggcatttctt gtatcgctga gatatacttc
    12601 tgccaactct tctaaataat ctttttgggc gatgaccgat tccatcgcat tagcaactaa
    12661 actcaattca tgagtgatat cgaatacaag agaagtttca aattcttttt tatcaccgat
    12721 agctttggct aataaagtta gtacagctat ttgtgcagta taagctttcg ttgatgctac
    12781 tgcgatttcg ggtccagcat gcaatagcag tgtaaaggat gcttcccgcg aaagagtaga
    12841 accagcaaca tttgtaatcg taagtgaagg gtagccgaga cggttgattt ttaccagcac
    12901 ttggcggctg tctgctgttt cgccactttg actcaagaaa ataaagaatg gtttttttga
    12961 aagaagaggt gtgttatagc caaattcact ggctaaatga acctcaacag gaatctgtgt
    13021 caatttttcc aaagtctgtt tacctgctaa ccccgcatgg taactagttc cgcaagcaac
    13081 gatatagatt cgatcgctat cagacaatgt atcgaccagc ttttgatcaa gagagacacg
    13141 attgtcttct gttacgtatt tttgagcgat acgacgcatg ataataggct gttcgtcgat
    13201 ttctttcagc atataaaaag gataggtacc tttctcgata tctgaagcat ctacttgtgc
    13261 ttcgaatgaa gaacgttcta cgatgtcacc tgcaaaattt tgaataataa ctttttctga
    13321 tgtgacggta accatctctt catcttcaat ctcaacgaat tccttcgtat gagcaagcat
    13381 agccattgca tcgctggcaa tcacattgaa tccatcgccg agtccgatta atagtggact
    13441 tttattttta gcgacgtaaa taacatcagg tgctgtccga tcaatcaaag cgaaagcgta
    13501 agaacctttg atgacccgca aagcttttaa aaacgcctct tgcgttgtag tatccttcgc
    13561 gaaagtttca attagatgag cgataatctc agtatcggtc tcaccaataa agtggtcatt
    13621 tgctaaataa gcaattttaa gttcttcaaa gttttcgatt acgccgttgt gaacgagcac
    13681 gaagcgtcca ctttgggaag tatgtggatg ggcgttttct tcagaaggtt gcccgtgagt
    13741 tgcccatcgt gtatgaccaa tacctatatt tcctgtagta tcaatattta ttttgttttt
    13801 caaattttga attctgcctt gtgctttgac cagataatca gttgtgccat ctgagacaaa
    13861 gataccagct gaatcataac cacggtattc taatttttct agaccgtcaa tcaatcctgg
    13921 tgttacattt tttaatccaa tcattccaac aattccgcac ataaaaaaca tcctactttc
    13981 tataatgaat tcgtatgaat gcttccagct gcaggaaaat ccttttgttt tatctttgca
    14041 gacatttttt gtggattttc tgtagagtgc agtccctagg gccatccgcc gaatctttcg
    14101 ataaaccctc tcctcgtcat ctgtttacac agacctggcg ctatcctaaa aggtataact
    14161 atcctccttt ttgtttttta tctggaaaca tttaaaatca taaccttttg cgaatgttct
    14221 tgtcaacaaa aaaagaataa aaaataaatt ggtatatatc aaattctttg tcaaatgtcc
    14281 tttttatgtc aaaaaataat gggtttttat ctatcatctt tttcaggagt tcggtacaat
    14341 agaaagtaat cggatagagg aggagtaaaa atggattcaa aaattgagat catgacactg
    14401 ggaatgttaa aaaaacaatt gtccaaattc gaagcatcag ctggtgtatc agatgatacg
    14461 aaaatatttt tagatactgg ctgggatagc atccaagaaa tcgcacctga tgccttggaa
    14521 gttgctcaag ctcgtgagtt tacggtagaa gatgaattaa caaaagagtc gttttccggg
    14581 tatgcgcggg aagaaaaagc agagcggttc gacacttctg agcagtcaga aactgtgatc
    14641 gtcataaaaa acttgtatta gaaaggagaa agaatgaata aaagacgttg ggcagcagtt
    14701 ctgattgctg caggattatt tgtggtttcg ttagtttcag ctagtttgac taagcctcag
    14761 gaaaacgatc aaattgaggg attgaacagt tggttatatg gggatgaaga actagctcca
    14821 aatgtgttgg aagagggaga cagtacgcaa aaaatcgtaa aattatcagt agatggaacg
    14881 attgctgcgg gagggtcttc aggtttattt gcttctgaag gttacaatca taaaaatttt
    14941 atggaacaac tgaaggcgat tgaagaagat ccaacagtca aaggtatctt tttagaagta
    15001 aattctcctg gtggcggtgt ctacgaaagt gcggaaatcg ctaagaaatt ggatacgatt
    15061 cgtaaagaac atgatatccc aatgtatgtc agcatgaaaa acatggcagc tagcggtggc
    15121 tactatattt ctgctcaagc agataaaatc tttgcaacag aagaaacagt gaccggctca
    15181 attggtgtga tcatgtcagg attgaattat tcaggacttc tagaaaaact gggtgtggaa
    15241 gatacaacag ttaaaagcgg agcgttgaaa gatatgggtt caggaacgcg accagagaca
    15301 aaagaagaag aagcggtgct tcaagcatat atcgacagcg catatagtcg ttttgtagca
    15361 atcgtcagcg agggacggaa taaatctgaa gaagaagtca aaaaaattgc tgatggaagg
    15421 atttatgatg gtgtccaagc aaaagaagta ggtttagtcg atgaattggg atttcctgaa
    15481 gatgctttaa aagctatacg aaaggaacaa cagcttgaag atgcggaact agtagaatat
    15541 tcttccacgc catctgggtt tgctagtaca tggttaggaa gtaaactagc agaattccag
    15601 ggattgaagc cttctgagac tagtcagatc ttatccgttc ttgagaatct agggacagcg
    15661 gaatcaccaa aagcaatgta ttattacgga ggtgagtaag catgtcagag ctttcaaatg
    15721 aaaataagga acaagaaaaa gataagcaac agcagcctgc tgtaggacct gactccaact
    15781 tttcccaaaa ggtactgagc tcttttgaaa gagagccttt gacgcccgaa gaaatcaaaa
    15841 agaaacaacg taaatggaaa aagtatacgg acacaagaaa tgaaccgcca aaagcaaatg
    15901 attttcctaa gtatttctat gcaggttttt ttatccgaat gtttgccttc ttggtcgatc
    15961 ttttgtgtat cggagctatt acgcgtttaa cacttggtat tgccaaaaat ctaggttggc
    16021 ttaatttttc agattcttat ctgagtctct acggattcac agccttactt atttatctag
    16081 cgtattttat tttattgacg aaattcaatc atggccaaac gattgggaaa atgatctttg
    16141 gtattcgagt catcagcttt gatgaagaag aacttagctg gtcaaccgtt ttagttagag
    16201 aaggtgcttg ccggttcatt ctaaaatttc ctttgctgat gttagggtat cttccagcag
    16261 tctttaatca gaaaaagcaa catctgggcg attttttcac taatacaagt gttgttacga
    16321 tcaatctgat caaagcgttc aaccaggaag tcaacagctg atgagcaata aaaagcagta
    16381 acagttgaaa gcaccttcta aaagtcagat ctaaaatcta gcttttagag ggtgcttcag
    16441 ttatttttaa ttgtcatgct ttttttgttt tgctactatt gaacatgagc tgaatatgag
    16501 caagaggagg acactgcgtg aagccgattg attttccaga aaaatatgag aatttaatgc
    16561 gagtggcaca acaagcacta gcaaaccagc aatatcagca ggcaaaagaa ttatttcaac
    16621 gagcttacga gttgaaagaa agctttgagg ctaatagcct gcttgttttt tgtctttacg
    16681 agttggacga gaaaaaagaa gcattgaagc aagcactctt gcatgaaaaa cagtatcttc
    16741 aaaatgagga gtttgctgaa ttttattttg acttgttaat cagtacacaa gattttttat
    16801 atgctagaaa attgatagca agtacagatt tttatgaagc tttcgagcag cgggtcattg
    16861 aaaaaatcca atttgcagaa gaattatccg gacaaatgga gaggcagaag atcaaggcac
    16921 tccacaataa atcggaggaa cttccttcgt tggaacctgc acgtcagctt gcgtctattg
    16981 atgagatcga acagcttcct tatcatgagt ttatccaaac agcgagtaaa cttattgtat
    17041 cgccagaggt ccatatttta gcaagggcaa aattactgga gacacttagg caattgaacg
    17101 aatgccatcc tgttttctat ttgacgattg aagaaaagtt agtcaaagtc ataccaaaag
    17161 atctgccaaa gccgcagcag caggcaagtt accagcaatt atgtgtattt tcagatcgtt
    17221 acgggaatga agatgcgctg cttagttccg tgttaaaaga agaattcacg ttgcaatctg
    17281 caattgttta tcctgtctat gatacataca tcgaagaccc gaagcgctgg tttcaactga
    17341 ctgtagaggc gtgtacagga aaaacgatgt atgaaggtac agaagatgaa aaacaagatt
    17401 ttttaaaaaa aagagaaaaa attttgcagc aaatgatctt cttccattag attttatcag
    17461 tgaaaaagat aaaaatgtta tgaaaaacaa ggattctatc cattcaatat gtttacaatt
    17521 tgaaagggtt agtgtactat ttaaaggtat gcatgaaacg tttttgatgt ataaaatgaa
    17581 tttctaaact cggagggaat taaatgactg caacatttga aaaaaaaggc acgaatgatg
    17641 gtgttttaaa atttacgatt gcgcaagacg aaatccaaaa aggattaaca actgcgttta
    17701 ataaagtgaa aaaaagcttg aacgtacctg gattccgtaa agggaaagtt tctcgtcaag
    17761 tattcaaccg tatgtacggt gaagaagctt tatatgaaga tgcattgaat gctgtattac
    17821 cagaagctta tgaagctgct gtcaaagaag ctggcatcga tcctgtttct caaccaaaaa
    17881 tcgatgttga aagcatggaa aaaggtcaag actgggtaat cactgctgaa gtaacagtga
    17941 aaccagaagt aaaattagga caatataaag atctagttgt tgaaaaacaa gaccgtgaag
    18001 taacagatgc tgacgtggat gcacgtatca aacgtgaaca agaagcacaa gctgaattag
    18061 ttatcaaaga agatgctgca gctgaaaatg gcgatacagt agtaatcgac ttcgaaggct
    18121 ttgtagatgg cgaagcgttt gatggcggaa aaggcgaaaa ttactcactt gaattaggtt
    18181 caggctcatt cattccaggt tttgaagatc aattagttgg tcataaatca ggtgaaagtc
    18241 tagatgtaaa tgtaactttc ccagaagatt atcaagcaga agatcttgct ggaaaagaag
    18301 cagtcttcaa agtgacaatc catgaagtaa aagcaaaaga agtgcctgaa ttagatgacg
    18361 aattcgcaaa agatgttgat gacgaagtat caacattaga cgaattgaaa gaaaaatatc
    18421 gcaaagaatt agtagaaaca aaagaaaaag cagcggacga tgcaaaagat gaagcagcga
    18481 tccgtatggc tgttgaaaac gcagaaatcg ttgaattgcc acacgtgatg gttcatgatg
    18541 aagtacatcg ttcaatggac gaattcttga acaacatgca acgtcaaggt atctctcctg
    18601 aaatgtacta tcaattaact ggttctacag aagaagactt gcataaacaa ttcgaaggtg
    18661 aagcagaaat gcgcacaaga acaaatcttg tgatcgaagc aattgcagct gctgaaaatc
    18721 ttcaagcaac agaagaagaa atcaatgcag aaatccaaga attatcagaa acatacaaca
    18781 tgccaatcga acaaatcaaa cgcgtgttga cagaagatat gctggaacat gatgtgacaa
    18841 tgaaaaaagc aatcgaattg atcacaggta cagcgacaga agcgaaagaa tagaaatcaa
    18901 gtaataaatc atttctgaag aagctacttc tagaaaattg tttattcgaa ataagggact
    18961 gtgacacgat tttgtcacag ttccttattt tttgttaaga gtaaaaaatg caaaggtata
    19021 ggtcagttga tcctttgtta aacacttttt ctttttacta aactatgtta tgatagtaaa
    19081 agactgtaaa gtctttgata gtttcttgca tactttttgc aagttaaggg gtgaagacca
    19141 tgtatgacaa tccaagtagc aatgagaccg tacgctgttc tttttgcggg aaaacccaag
    19201 aagaagtgaa aaagatcgta gctggtccag gggtctacat ctgtaatgaa tgtatcgacc
    19261 tatgtaaaga aatcattgat gaagaatttt atgaagaagc tgtacgcgaa ttcactgaag
    19321 tccctaaacc actagaaatt cttgaagtat ttaataacta cgtgattgga caagatcgcg
    19381 caaaacgctc gttagctgtt gccgtataca atcactataa acgtgtcaat cagcaagcac
    19441 aagaaaacac agatgatgtt gaactgcaaa aaagcaatat ctgcttgatc ggacctacag
    19501 ggtctggtaa aacattttta gcacaaacat tagcaaaaac attgaatgta ccttttgcaa
    19561 tcgcagatgc gacaagtttg acagaagctg gttatgtggg ggaagatgta gaaaacattt
    19621 tgcttaaatt gcttcaatct gctgattaca atgtggaact tgcagaaaaa gggatcatct
    19681 atattgatga gatcgataaa attgcgcgta aaagcgaaaa tgtatctatt actcgtgacg
    19741 tttccggcga aggtgtccaa caagccttgc tgaaaattct agaaggaact gttgcaagtg
    19801 tgccacctca gggcggacgt aaacatccac atcaagaatt tattcagatt gatacgacga
    19861 acatattgtt catcgtaggt ggtgcatttg atggcatcga aacgattgtg aagaatcgta
    19921 tgggcgaaaa gacgattgga tttggtacac aaaaccaaaa actttctgaa gacgaaagtg
    19981 tcatgcagca tatcattcct gaagatttac taaaattcgg attgatccct gagtttatcg
    20041 gacgattacc tgttatggca gctttagaga aattgacaac agatgatctg gtacggatct
    20101 tgactgagcc gaaaaatgcg ctagtcaaac aatatcaaaa acttctttct ctagataata
    20161 ccgaacttga attcgaacct gaagcgttga gagccattgc gaaaaaagca atcgaacgca
    20221 ataccggtgc tcgaggactt cgttccatca ttgaagaaat catgatggat gtgatgtttg
    20281 atatcccttc aaatgaaaac atcgaaaaag tgattatcac aaaagaagca gctgaattgt
    20341 ctggtgagcc aacagtaatc tataaagatt cagagaaaaa agcgggttga tgaaaagagg
    20401 ctgccgtaaa agtcattttg gcttttgcga cagccgcttg ctttattcat caccttgtta
    20461 aggagagaga ccatgaaagt ccatcaagct gaaattatca taagtgcagt ggcaccaaaa
    20521 caatatccag atacacaatt gccagaaata gctctggcag gacgatcaaa tgtagggaaa
    20581 tcttctttta tcaacacatt gattgatcga aagaatttag cccgtacttc cggtaagcca
    20641 ggaaagaccc agacattgaa tttttatttg atcgagaacg cattacattt tgtcgatgtt
    20701 ccgggatatg ggtatgcaaa agtatccaaa actgagcgag caaaatgggg aaagatgatc
    20761 gagacatatt taacgactag agaccagcta cgtgcggtcg tttctttagt agatttgcgt
    20821 cataagcctt ctcaagaaga tatccagatg tatcaatttt tgaagtatta tgatatccct
    20881 gtaattgttg ttgccactaa ggcagataag atccctcgtg gtaaatggaa caagcatgag
    20941 agcgaaatca aaaaagcgtt ggattttgat cgcaatgaca cgtttattct cttttcttcc
    21001 gttacaaaac agggaaaaga agaagcgtgg gcagcaatcg aacaagcaat tgaatcataa
    21061 aaagaataaa aaagacttac cagtcaaact ctttcataaa agtttggctg gtaagtcttt
    21121 ttttgagctt aacttacttt tgtttcttgt cttttttctt ttcattttgt ggctgtggtt
    21181 ttagaaatcg atcgatctct tcttttggtg caactgtttt ttccggatcg acagcaaatg
    21241 attcgaaaag tttatctaaa ttatctgtac gttcaaattt caaccccatt gtaaacacct
    21301 ctttgtttct ttttacttag tctatcatgt aatcgtccaa attggtttct aagacataaa
    21361 gttttagttt ttctttatca ttggttataa ttaaattata aaaagatttg aaagaggtga
    21421 gaaaatgatt ggcgaagata aattattgat gactatccag ctcacagact taaatgaaga
    21481 agaaaaaaaa caggaaataa gcgattgggc tattttgaca cgtctgttga tccagccaac
    21541 atttatccgc tctttcacac aacaagcgga tccgtatgat cttcgaaagc ttcaagaaaa
    21601 aataatgcta gcatcggtaa gagacgaaag ttggttagtt gtaggaaatg acgagaacga
    21661 atgtagtttt cgcttggaag ataatcaatt attgataaaa aatgtattgt ctatctctgt
    21721 tttcaaagaa aagcaatttt tgattcgaga cttcattcag aaaaaaatga ttgctcatgg
    21781 ggtgttcgcc tatatgcgag cttatagcga gtttatctat cacaatacaa aacagatctc
    21841 tcaacggcta ttgtttgaga aaaaggatga aatcgaacat ttacctaaaa tgaaacaaca
    21901 aaacggggaa gtagtagtcg attgcaacca atttccagga tacgatcttt tttaccaagg
    21961 tttatgtttc acttcgtgtt gggaaatgta ttatagtcgc tattatcatc aaatcatccc
    22021 taaaccgatt tttctcgaca tccaacaagt agaaaaagtg aaggaattag aaaacgaagt
    22081 catatgtatc cagctttatc gagacccctt taactggcaa aagccaaaca atcaaatgtt
    22141 tcaatcctat tttagggacc agcttgggtt tgatcatctt gcttgggaca atggtgtcgg
    22201 tttattgaaa ccgccattcg ttgaatacat ttacactgac catacgatcc aatctgtcca
    22261 gtatcagaat gcacaaatgc aacctgttcc taaaaagaat gcttcatttt ttgtaacaaa
    22321 aagttatgat atccaacaag gagattataa agaaaggcgt gttcgcggaa cactaaatgc
    22381 tcaggcgtat tttccttggg ttgacgagaa tcgatcacgc atgatgtgct ataaagttat
    22441 cgatccgact gttgctttgg ataatggaat cgaagcttat tgttattata ttagggaata
    22501 tctagaagta gaggtaacag atgaaaaata tcaagagtat cttttatcct taagaatcta
    22561 tgttccttca gaatccctgt cagaattacc gttaaaggaa atcaaacata gactctctga
    22621 tgttaccttc aaacgaataa agaaaaagcg gcgaagtatc caattcgatg tcaagaaagg
    22681 tgagcagcat ctgcgtgtgc aatttttaga ttaccgagaa ttagaacaat tgattacttt
    22741 acaaaaaata tgaggtgaag aaaatggata aacaactcta ttatcgaata atggttccgg
    22801 catcattatt atccgtctgt ctcttttttg ttcctaattt tcctattcga tcagtcatcg
    22861 gttttttggt catgtttggc gggatgggac tttattatta tcttcttcat aaggaaagaa
    22921 aacctgcttc tgtttatatg aaaaaaacaa gaagcgaaaa taaaaaacga taaaacggct
    22981 tgatgataga caaaaaaata aaaaaccagt ctttctgact ggttttttat tttttt
//
LOCUS       DACDSY010000028        22854 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDSY010000028 DACDSY010000000
VERSION     DACDSY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.028"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            62..286
                     /gene="nrdH"
                     /locus_tag="IWK44_000944"
     CDS             62..286
                     /gene="nrdH"
                     /locus_tag="IWK44_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP9549487.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            445..2604
                     /gene="nrdE"
                     /locus_tag="IWK44_000945"
     CDS             445..2604
                     /gene="nrdE"
                     /locus_tag="IWK44_000945"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP9549488.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2654..3619
                     /gene="nrdF"
                     /locus_tag="IWK44_000946"
     CDS             2654..3619
                     /gene="nrdF"
                     /locus_tag="IWK44_000946"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP9549489.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3708..4847)
                     /locus_tag="IWK44_000947"
     CDS             complement(3708..4847)
                     /locus_tag="IWK44_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9549490.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5156..5869)
                     /locus_tag="IWK44_000948"
     CDS             complement(5156..5869)
                     /locus_tag="IWK44_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP9549491.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6123..6824)
                     /gene="nagB"
                     /locus_tag="IWK44_000949"
     CDS             complement(6123..6824)
                     /gene="nagB"
                     /locus_tag="IWK44_000949"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP9549492.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7058..8590
                     /locus_tag="IWK44_000950"
     CDS             7058..8590
                     /locus_tag="IWK44_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9549493.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8781..9983)
                     /locus_tag="IWK44_000951"
     CDS             complement(8781..9983)
                     /locus_tag="IWK44_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9549494.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(10065..10754)
                     /locus_tag="IWK44_000952"
     CDS             complement(10065..10754)
                     /locus_tag="IWK44_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9549495.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(10860..11228)
                     /gene="rplL"
                     /locus_tag="IWK44_000953"
     CDS             complement(10860..11228)
                     /gene="rplL"
                     /locus_tag="IWK44_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP9549496.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(11281..11784)
                     /locus_tag="IWK44_000954"
     CDS             complement(11281..11784)
                     /locus_tag="IWK44_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP9549497.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(12194..12883)
                     /gene="rplA"
                     /locus_tag="IWK44_000955"
     CDS             complement(12194..12883)
                     /gene="rplA"
                     /locus_tag="IWK44_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP9549498.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(12987..13409)
                     /gene="rplK"
                     /locus_tag="IWK44_000956"
     CDS             complement(12987..13409)
                     /gene="rplK"
                     /locus_tag="IWK44_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP9549499.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            13752..14420
                     /gene="sdaAB"
                     /locus_tag="IWK44_000957"
     CDS             13752..14420
                     /gene="sdaAB"
                     /locus_tag="IWK44_000957"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP9549500.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            14433..15305
                     /gene="sdaAA"
                     /locus_tag="IWK44_000958"
     CDS             14433..15305
                     /gene="sdaAA"
                     /locus_tag="IWK44_000958"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP9549501.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(15396..16415)
                     /locus_tag="IWK44_000959"
     CDS             complement(15396..16415)
                     /locus_tag="IWK44_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9549502.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(16588..17475)
                     /locus_tag="IWK44_000960"
     CDS             complement(16588..17475)
                     /locus_tag="IWK44_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9549503.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(17552..18097)
                     /gene="nusG"
                     /locus_tag="IWK44_000961"
     CDS             complement(17552..18097)
                     /gene="nusG"
                     /locus_tag="IWK44_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP9549504.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(18198..18368)
                     /gene="secE"
                     /locus_tag="IWK44_000962"
     CDS             complement(18198..18368)
                     /gene="secE"
                     /locus_tag="IWK44_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP9549505.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(18392..18544)
                     /gene="rpmG"
                     /locus_tag="IWK44_000963"
     CDS             complement(18392..18544)
                     /gene="rpmG"
                     /locus_tag="IWK44_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9549506.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            18706..19350
                     /locus_tag="IWK44_000964"
     CDS             18706..19350
                     /locus_tag="IWK44_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9549507.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(19406..19909)
                     /locus_tag="IWK44_000965"
     CDS             complement(19406..19909)
                     /locus_tag="IWK44_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9549508.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(19922..19968)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            20506..21471
                     /locus_tag="IWK44_000966"
     CDS             20506..21471
                     /locus_tag="IWK44_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9549509.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(21570..22742)
                     /locus_tag="IWK44_000967"
     CDS             complement(21570..22742)
                     /locus_tag="IWK44_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9549510.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(22729..>22854)
                     /locus_tag="IWK44_000968"
     CDS             complement(22729..>22854)
                     /locus_tag="IWK44_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9549511.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         7145 a         4587 c         4108 g         7014 t
ORIGIN      
        1 tactaagagt tttatactat ctattataga taataagaat gtgttagagg aggcactcac
       61 catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa tgtaagatgg tcaaacggtt
      121 tttaacagaa aatcaaattg cttttgaaga agtcaatatc gatgaacagc ctgaagcagt
      181 agactggttg aaagcacaag gattccaaag tgttcctgtc attacttctg atgcagaaac
      241 agttatcgga ttccgtcctg atcagttgcg taaacttgcc agctaacatg actaaaagaa
      301 tcaaaacgtg atacatggat ttgaagggct tgagacagca ccagtttttg tcgcaaactt
      361 ccaaatcctt ttttaaagct taaaaacaat gcaatgattt gcaatcaagc atcatcaaat
      421 acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt tacaagatgt cagttatttc
      481 aagctgaata atgaaatcaa tcggccagtc aatggtcaaa tccctttgaa taaggataaa
      541 gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa atacgatgac ttttctatct
      601 attatggaaa aaatcaatta cttgatcgat caagaatata tagaagcaga attcattcaa
      661 ttgtattcac catcatttat tgaaaagcta tatcaatttt tggacgagca acatttttct
      721 ttcaaatctt ttatggctgc ttacaaattt tattctcaat atgcgttgaa aaccaacgac
      781 ggttccgctt atctggaaac ttatgaagat cgagtagcct tcaatgcgct ttactttgcg
      841 aacggggatg aagaattagc acttgctctt gcagacgaaa tgatccatca acgatatcag
      901 ccagctacac cttctttctt gaatgctgga cgtaaacgtc ggggcgaatt ggtgtcttgt
      961 ttcttgactc aagtcactga cgatatgaac tctatcgggc gctcaatcaa ctctgctttg
     1021 caattgtccc gtattggtgg cggcgtaggg attactttat caaatcttcg tgaagccggc
     1081 gctcctatca aaggatatga gggtgctgca agtggtgtcg ttccagtcat gaaattattc
     1141 gaagatagtt tcagctattc taatcaatta ggtcagcgtc aaggagctgg cgtggtttat
     1201 ttgaacgttt tccatccaga cattattgct ttcctttcag caaaaaaaga aaatgcagat
     1261 gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg tacctgataa attttatgaa
     1321 ttgacacgta aaaacgaaga tatgtattta tttagtccat atagtgtcga aaaagaatat
     1381 ggtgtcccat tctcttatgt aaatattaca gctgagtatg atcatctagt tgcaaatcca
     1441 aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa atgaaatctc aaaattacaa
     1501 caagaatctg gctatcctta cgtaatcaat attgatacag cgaaccgcga gaatccaatc
     1561 gatggcaaga tcatcatgag taatttatgt tcagagattt tacaagttca aacgccttct
     1621 gtgattaacg gcaaacagga atatgaagta ttaggaacgg atatctcatg taacttaggc
     1681 tcgacaaata tcgtgaattt gatgcaaagc cctaactttg gtcaatctgt gcgtacgatg
     1741 actcgtgcat tgacatttat tacagatgca tctgatatcg atgtcgtacc accgatccaa
     1801 aacggaaata agctgaatca tacgattgga ttaggtgcca tgggcttaca cacttatctt
     1861 gcaaaagaac aaatggaata tggctcagag gattcattag actttacaaa tatttacttc
     1921 atgctgttga attattggac acttgttgaa agcaacaata tcgctcgtga aagaaaacaa
     1981 gttttccata attttgaaaa atcagcttat gctgacggca cttatttcga aaaatacgta
     2041 actggagaat tccagccaaa atcagaaaaa gtcaaagcat tgtttgaagg catttttatc
     2101 ccaactgctg aagattggaa tgctttgaaa caagcagtaa tgaaagatgg actgtatcat
     2161 caaaatcgct tggcagtagc accaaatggc tcaatttcat atatcaacga tacgagtgca
     2221 agtattcatc ctatcactcg catgatcgaa gaacgccaag agaagaaaat cggtaaaatt
     2281 tattacccag cgccttatct atccaatgag acgattccgt attacacttc tgcttatgat
     2341 atggacatgc gtaaagtaat cgatgtttat gcaatggcac aacaacacgt cgatcaaggc
     2401 atgagtttga ctttattcat gcgttctgaa atcccagaag gcatttatga atggaagaca
     2461 acaacgaagc aaacgacacg tgatttgaat attttaagac attacgcttt ccacaaagga
     2521 atcaaatcca tttattacgt acgtaccttt actgatgatg cagaagaaat cggaagcaat
     2581 caatgtgaaa gctgcgtgat ctaatatcat agatagattc gcttattcat attttaatta
     2641 aaggagcatt ctcatgtcag aaacatatta tgaagcaata aactggaatg aaattgaaga
     2701 tatcattgat aaatccactt gggagaagct gacagagcaa ttttggctag atacacgtat
     2761 cccactttca aatgacctag atgattggcg tacattgtct caattggaaa aagatacagt
     2821 aggacatgta tttggcggtt tgacactgct agatactgtc caatctgaaa gtggcatgga
     2881 tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc gttttgaaca atattcaatt
     2941 catggaatcc gttcatgcca aaagttattc ttctattttt agtacactga atacgaaaaa
     3001 agaaatcgaa gaaatttttg actggacaaa tacgaatcct tatctgcaaa aaaaagctga
     3061 gcgtatcaat gaaatctaca aacacggtac accgctagaa aagaaaattg ccagcgtctt
     3121 tttagaaaca ttcttgttct attcaggttt ttatactcct ctttattatt taggaaacaa
     3181 caaattggct aacgtagcag agatcatcaa actaatcatc cgagatgaat ctgtacatgg
     3241 aacctacatc ggttacaaat tccaattagg attcaacgag ttacctgaag cagaacaaga
     3301 aacgttaaaa gactggatgt acaatcttct ttatgagctt tacgaaaacg aagaacgtta
     3361 tacagaagaa ttatacgatc caatcggctg gacagaagaa gtcaagacat tcctacgcta
     3421 taatgccaac aaagcattga tgaacctagg gatggatcca ctctttccag atactgccaa
     3481 tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc acaagcaacc atgacttctt
     3541 ctcacaagtc ggaaacggct atttgttagg tcatgtagaa gcgatgaaag acgacgatta
     3601 tttgattgga ttggaataag gatttatttt aattcaaaaa tttttcgaaa aataaaaaga
     3661 gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta ttcacttgtt
     3721 tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct tctcaataaa
     3781 tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg tatgcaaatc
     3841 agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat gatcgtcaaa
     3901 ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc atctaattgt
     3961 tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat aaacaaaccg
     4021 acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat attcgtgatt
     4081 ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat tgccaaaaag
     4141 ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc agataatgtt
     4201 tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg aaagaagtga
     4261 gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat aaatggaacg
     4321 gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac acttgcagat
     4381 aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga tgctaattgt
     4441 tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa ccatttgctt
     4501 gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc cattaactga
     4561 ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa cgtcaataaa
     4621 aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg atcgaacata
     4681 taatagataa aaccagcaac aagcaatggt aaaaaaactg ccgaaagcat tttcaatatt
     4741 ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt gagtactagc
     4801 tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca tctcctccaa
     4861 ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag attttcattt
     4921 gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac ttaaacaaaa
     4981 acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa taaacggtgt
     5041 tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg aactattttt
     5101 atttggctgg gcttattact tgatgtagga catctatttt acaacctcag atcatttatt
     5161 gcgcaatact gactaaatgc aactttgtga tatttttgtg tacggacaac tcctcgatca
     5221 catcggcata agtgagccct tttggaagat caatcgtata gatatttttg tacactcggt
     5281 agtcatcaac gaattttacg tcaaaattca catcttcgat tacgatattt ttctcttcaa
     5341 aataatcggt aagaaaatct ttggtttctt ttctatggat aaattggatt tctaactttt
     5401 tagtcgtagg tacatggatc acgcgtttca ctactgtgag tgcaatagca atcccgataa
     5461 aactgatgat cgcaatggaa tagtaaccca tgccgatggc gatccctaat ccagcaattg
     5521 cccataaaga agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg atgatcgttc
     5581 ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat cagccttgct tcatctgaac
     5641 gaatcgttcc agcaagctgt ggatactcta aagcatctct taatgcacca ctagcgatct
     5701 caacttggat caatgcaatg atacatgctc ccatacaaac taaaatatgc gtacgcatac
     5761 ctgctggacg atttttatat tgtcgttcaa aaccgattgc tccgccaaat agcatagcga
     5821 tccctaaacg taaaataatc tctggaattg tcaataccat atcattcatc taaacaccct
     5881 ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt ttacctctta
     5941 cgaaaaaagg actgtgacaa aatttttgtc atagtccttt ttatgaataa acggtgttca
     6001 atcagctgac cctcggtatt actcaacaaa cggcaaaata tggggagcta tttttccttt
     6061 gtcttctcta atactcaggt caaaacgctg atttcacaac ctctttagcc tactattgaa
     6121 aattacaact tacttgctgc ttcttcatcg atgatcacaa caacattgtc gtgattttgc
     6181 aatacacttg ctggcaaatg attcgttaca ggtccttcaa tcatcccttt gattgcatca
     6241 gctttgcttt cgccaaatgc catcaagacg atttctttac ttttcataat tgaaccgatt
     6301 cccattgaaa cagcttttgt tggtacatct tctgctttat cgaagaatcg tttgtttgca
     6361 ttgattgttg attctgttaa gtcaactact gaagtcgttc cgtcaaatga agcaccaggt
     6421 tcattgaaac cgatatgtcc attttgcccg attcctaaga tttggatatc gattggatgt
     6481 gcatcgatga tttgatcata ctttttgcat gctgcttcca gatcttctgc ttttccatct
     6541 ggtacaaacg tttctttgaa tggtttttta tcaaataatt ggacatccat gaaatgacgg
     6601 tagctttgtt catctgaacc gcctagtcca acgtactcat ctaagtttac agaagtcatt
     6661 tcactgaaat ccaaatcgct tgaagtcatt tctttatata aagtaactgg tgtgcttcct
     6721 gttgccaatc ctaatacttt tgcacctttg ttcatacctt ctttgatgat ttcaaatgct
     6781 ttttttccgc cttcttcagc gttttttaca cgaatgattt ccatagtgat tgctccttta
     6841 tattttggta tagcccaatt atagcattct ttttttcttt ttacaataat tagtatagac
     6901 caaattgcat ttttttcatt ttcttgacaa taaataggaa tccgtgttag gtttcataag
     6961 atggttcaat caaacaatcc atgttgcatg aaaaccaagc aagtgattca ctgtggacgc
     7021 tttatgtctg cagttttttt atgaaaaagg agttattatg agtatactga cattaaacaa
     7081 cattacccag caatttggcg acaaaatcct ctatgaaggt gtcgatctgc aattaaatgc
     7141 tggcgaacat cttgggctga ttggacaaaa cggagccggt aaaagtacat tgattaaaat
     7201 catcactggt gagatcttac cagatgacgg acagatccat tggcaaaaaa atattcacaa
     7261 aggttacctc gatcaatatg tggaagtcga tgaaacatta acaatcgaag aatttctaaa
     7321 aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt gcttttcttt accaaacata
     7381 tagcgagacc ttcgatgatt ccttgctgga aaaagctggg aaactccaaa cggaattaga
     7441 ccaaggagtg ttttatcaaa tcgacacctt agttgctgag atgagcagcg ggttggggat
     7501 cgatgtctta ggtttagatt cactgttgaa aaatctaagt ggtggacaac gttcaaaggt
     7561 gattttagca aaactacttt tagaaaaccc agatgttctg atcttagatg aaccaaccaa
     7621 tcacttagat gatcagcata tccaatggct gacgcagttt cttcaggatt ttgacggtgc
     7681 gtatatcgtg atttcccatg accaagaatt tcttgatcag atcaccaccc atatcgcaga
     7741 catcgagttt ggaaagatta ctaagtatac tggccatcta aaacaagcat tgaaacaaaa
     7801 ggaacaaaat cgcgaaagct atttgcgcca atatcatgct caacaaaaac aaatcgaaaa
     7861 gaccgaagct tatattcgaa aatacaaagc cggttcccga tcaacgatgg caaaaagccg
     7921 acaaaaacaa ttagataaaa tcgaacggct gacgcctcca gcttcagcaa gtaaagctgt
     7981 ctttgatttt ccattctcgc ctattgtcac gacattagca gtggaaacaa ctgatttagt
     8041 catcggatat gatcgaccgc tacttgcacc catcaatttg accatccgct ttggtgaaaa
     8101 tattgccatt cgaggcttca acgggattgg aaaatctact ttattaaaaa cactgatcgg
     8161 tgagattgaa aaaataagtg gcgatttcca cttcccagat aacacaaaaa tcaattactt
     8221 ttcacaagat ctgtactggg aaaatccatt agaaacacca ctacaatatt taagcagtca
     8281 atttccaaaa gcgactatca aagaattacg tcgccaacta gctaaagcag ggttagttaa
     8341 tcaattagca agtgaacctc tcactacatt aagtggtggg gaacaaacta aggtgaaact
     8401 tgcacagttg acaatgaacc aaggtaattt actgattctc gatgaaccga cgaaccatat
     8461 cgatcaagaa acgaaagaaa gcttacaaga cgggatacag aaatttccag gaacagtaat
     8521 catcgtctct catgaacaag aattttatca agatcttgtg caacgagtca ttgagattga
     8581 accaaaataa acgtttgatt gaatagttga aaaaaaggag ctgtgacaaa agttttgtca
     8641 caactccttc ttctgaataa atggtggcca attagctgac cttcggtatt agctcaaaaa
     8701 acggcaaaat atgaggagct atttttattt ggctattctt attacttgat gcagaacagc
     8761 ttttcacaac ctcttttcgt ctatttttta ttttcgagat ttctctttcc tttaaaaaca
     8821 actgtttgaa agaagaacgc acatccttga aaaacaagcc cgctaaccgt tacacctaac
     8881 cagccatact gctgccacat tgtcgtccct agcaatgaac cagccgcacc tccaataaag
     8941 tacataaaca taaatacagt attatttcgg ttgcttgctg cttcacctag cccttgtact
     9001 cgtgtttgat tggcgacttg accaaattga ttcccgacgt ctaataaaat aatagctaaa
     9061 agcaaaatgg ccaaatggct tccgccaacc aataaagcaa taaagcttat tgtttgcata
     9121 acaagtccca tatacacgat ttttctttca gaataagaat ctgccagtcg tcccacgata
     9181 ggtgcagcaa ctgctccaga caaaccaaaa atcgctaata tccctgcttc gaatgttccc
     9241 caattgtagg cgggactgct gatataaaaa atcaacgtag accaaaagat cgaaaatgtt
     9301 ccaaacatga aaaatccact gactgccgct tctcttaata gacgttgaga agcaactaaa
     9361 tgcggcaaac ttttaagtga atcaaggtat gaaacagctg tactactttg ctggacagaa
     9421 cgcggcattt tccagtaaag cagaagtgta agaattccga ctgcacataa agcaaataaa
     9481 tagatcatcc gccaagaaaa aagactggca acaataccag ataccgtccg agaaagcaaa
     9541 atccccacga gtaaaccgct aagcaatgta cccatgacct tccctcttgc ttttggtccc
     9601 gctagtaccg caccgtaagg aatgatgatt tgcggcacga tcgaaagtaa tcctattaaa
     9661 aaagaagcgc atgcaaataa cgtaaacgaa ggcgcaaaaa aagcggccaa cagagagacg
     9721 gcagataacg ctgccattcg aatgatcaat tttggacgat ccaccacgtc ccctaaagga
     9781 actaaaaaca gtaaccctaa cgcatagcct aattgggtca acatcgtcaa tatcccgact
     9841 gcacttgtgc taacagataa actagctgct acttttgttc caatcggctg gatgtaatac
     9901 atattggcaa caacaacgcc acaagtgatt gataatagta aaataagcga agttgttaaa
     9961 ggtttttttt cattactctt cattgatttc ccccatttcc tatcttagtt tacaacttat
    10021 ggaaaagatt ggaaatagaa aaatacaact attcgccaca cttattattt gtgcttttgt
    10081 tttccttgtt ttttcttgcg atctttataa atgaagacaa ctaaccctac tagcacagta
    10141 acacttgcaa ccaacgtgac tgcagttttg ttatatccgt gaaacagagc ttctccacca
    10201 cacgcataca acacagacga agggatgact ccaatcacga tcgacaatat gatttgctgt
    10261 agaggcaatt tcatcgcatc tgcagcaaaa ttgaccatag aagatggaat aaccggtatc
    10321 atatagccta acattacacc tatttttgga tgtttcattt gtcggattgc ttgcacccaa
    10381 tgattcgttt cggttttctt atctaaaaat tttaagcgat gcataaggaa aattgcagtg
    10441 atgttaccaa gagcattacc caaaatgttg atgatcgcac ctgtcagtgg tccaaagctc
    10501 aaaccagcaa tgactccaac tactgaagta ggaatacctg gaacagcaca catcaccgct
    10561 gtcaatacga tcaagagtat cgatgcaaaa gtcccatgcg aacgaatatg tgcgagtagc
    10621 tctgccttac tcgctctcgg atttaaaaat aattggatgt ctgtctgata ttctaaatac
    10681 aaccgatagc ccactaccag tatcagaatg attcccaacg agatcaatat caatcggatt
    10741 attttctttt tcatatttcc tcctgaaaaa aatctatatt cattaaataa tagtttaaca
    10801 taaaaacaaa aaagcaccga aatccaacat aggatctcag tgcttttaaa tagtaaaaat
    10861 tatttaacag ttacagatgc gccaacttct tcaagtttag ctttcaattc ttcagcttct
    10921 tctttagata cgccttcttt aactggtgca ggagcgccat caactacagc tttagcttct
    10981 ttcaagccta aaccagttgc ttcacgaact gctttgataa ctttaacttt ttggtctcca
    11041 gctgcagtta attctacagt gaattctgtt tgttcttcag cagcagcagc gccaccagca
    11101 gcagctacag ctacaggagc agcagcagat acgccaaatt cttcttcaat agctttaact
    11161 aggtcgttaa gttctaggat agttgcttct tttaactcag caacgatgtt ttcaatgttc
    11221 aatgccatta taatttcctc cgtttaattg ttctttttta tttaatgctt tatgcggcaa
    11281 ttaagctgca tctccatctt ctttgtctgc cactgctttg acagcgtaag ccacgttgcg
    11341 gactggcgct tgtagtacag atagcaacat agatagaagt ccttcgcgat ttggcaattt
    11401 tgccaatgct gtgatttctt ccaatgaaga gactttacct tcgatgatac cgcctttgat
    11461 ttctaatgct tttgcttctt tagcaaattc gtccatgatt ttcgctggtg cgactacatc
    11521 ttcgttactg aatgcaacag ctgtaggtcc agtgaatact tcgtccatgc cttcaaggcc
    11581 agctttttta gctgcacgtg aaagaataga atttttgata actttcattt ctacgttagc
    11641 atcacgaagt tgtttacgta aacgtgttac ttcatcaact gttaatccgc ggtagtcaac
    11701 gactactact gatgcagctg ctgtaaattt ttcgcttact tcatcaacga ttgctgcttt
    11761 ttttgcgata attgcttcac tcatttttga gtttcacctc ctgaattttg atggaagagt
    11821 tttttgcact gaaaaggcaa gtcactttta tagttacttt gctcaaccag tattattcat
    11881 cacctattca cttcgttcat agtgattcta ctcgctttgc tcgaaaggaa actcctttcg
    11941 ctttgctcaa ccagtattgt tcatcatctc ttcacttcgt tcatagtgat tcacaataaa
    12001 aaactctatg ccaccgcaga catagaggga ctattgaata tattcaataa acgtcctcgg
    12061 taggaaatta aggcttgatg ccacctactg tcttcggtac aagttaatta ttaacctcaa
    12121 ctagcttagc atgctaaact agttgagtca agaattattt ttcagttgga tcaatcaaca
    12181 ttctaatatt taattagaaa gaagcttggt caacatggat accaggtcca aatgttgttg
    12241 tgatcgttac atttttcatg tattgacctt tagctgctga tggtttagct tttagcaata
    12301 catcatggat tgctgcaaag ttttcaacta atttttcatc gtcaaatgaa acttttccga
    12361 ttggtacatg gatatttcca gctttgtcca cacggtaagt tactttacca gctttcactt
    12421 cgtttactgc ttttgttacg tccattgtaa ctgtaccagt tttagggttt ggcattaagc
    12481 cttttggtcc taatactcgt cctagacgac ctacagtagc catcatatca ggtgttgcaa
    12541 caactacatc aaagtcaaac catccgtttt ggattttttg aaccatgtcg tcgtcaccta
    12601 cgaaatctgc accagctgct tcagcttctt ttgctttgtc tcctttagca aaaactaaaa
    12661 cagtttgtgt tttaccagtt ccgtttggta atacaacagc accacggatt tgttggtctg
    12721 cttttttagg gtctacgttc aaacggtatg caacttcaac tgttgcatca aattttgcaa
    12781 tgtttgtttc ttttgctaaa gctactgctt cggcaacagg ataaacttta cttgaatcaa
    12841 ctttttttaa tgcatcttgc atttttttgc tctttttagc cattttggtt cctccttgat
    12901 tgtggttata acggttgtac ctcccacgtg ttctatatct ttcaatatag agccaactga
    12961 gaagctgctg tcttagggtg tatagattat tctacagtga tccccatgct tcgtgcagtt
    13021 ccttcaacca tgcgcattgc tgcttcaacg ttagctgcgt ttaggtcttc catttttaat
    13081 tcagcgattt ctcttacttg atcgctagaa actttcgcaa ctttattttt gtttggttcg
    13141 cctgaacctt tttcgatttt agctgctttt ttcaatagaa cagcagctgg cggtgttttt
    13201 gtaacgaatg tgaatgaacg gtcttcgtat acagaaataa caaccggaat gatcaaacct
    13261 gcttgatcag ctgtacgagc gttgaattct tttgtgaatc ccatgatatt gatacccgct
    13321 tgacctagtg caggacctac tggtggagct ggagttgctt tacctgcagg aatttgcaat
    13381 ttaacgattt tttctacttt ctttgccacg agacatacct ccttaagtcc gtgatgtggt
    13441 taattggaga tgcatatttc tcctcccacg ttttatctga tctgaaaaaa tccagacaaa
    13501 taatgtgcaa aacaatgcac actggtacag tatagcaaat ttatttgttt tttcaagatt
    13561 tttttctgct atttttacat tttgcttgaa taaacgctaa atttattcta aaaaaacaac
    13621 aatttttcgt tctcatttct tcttttatgt tacacttgcg ctagcatggt tcaacagatg
    13681 aaagcaaaca ataacacggt tttttagacc cttgtacaaa gattcctaca taacaaaatg
    13741 aggtggagaa aatgaaagat ttaaggtata gaagtgtctt tgatattatt ggacctgtga
    13801 tgattggtcc aagtagctct cacactgccg gagctgctag aatcgggaaa atcatacgta
    13861 gtatatttgg cgaacagcct gattctgtag atatttatct atatgaatca tttgcaaaaa
    13921 cctatcgggg gcacggaaca gatattgcat tagttggcgg cctgttaggg atggaacccg
    13981 atgatgaacg tttagctgat tctttgaaat tggcatatga agccggtatg gaagtcttgt
    14041 ttgttcctaa gagtgaaaaa gccgaacatc caaactctgt aaagatgctt ctaaaaaaag
    14101 gaacacgaaa gctttcagtc actggcattt cgatcggcgg tggcaatatc cagatttctg
    14161 aattgaatgg attcaaaatt tctttaagca tgggtactcc taccttgatc gttgtccacc
    14221 aagatgttcc aggtatgatt gctaaagtga ctagtatact ttctgagacc aatacgaata
    14281 tcggcactat gacagtgaca cgggaatcaa aaggtgaaaa tgccattatg ataattgaag
    14341 tagatgatcc tcaagtagaa gaaacggtaa aaagactcaa acagctgcca aatatcgaca
    14401 gcgtaaatta tttcgattaa ggagctaaaa aaatgttttt atcgattgaa gaattagtag
    14461 atcaagcaaa agcttatcca agtgtagcag aattgatgat tgcagttgaa atggaaatga
    14521 gtggccgttc ccgtagtcac atcatcgaga tcatggaaaa aaacctctca gtaatggaac
    14581 aatcaatcaa cgagggaaaa gaaggtgtta cctccgtcac aggaattacc ggtggtgatg
    14641 caaaaaaatt agatgattat ttaaatgagg cagacttttt aagtggagaa acaatcttaa
    14701 aagctgtacg caatgctgta gctgttaatg aagtcaatgc aaaaatgggg ttgatttgtg
    14761 ctacaccgac tgccggaagc gcgggtgttg ttcctggtgt gctgatggct gtccgagatc
    14821 ggcttgattt gacacatgaa cagcaattgg aattcttatt taccgctggc gctttcgggt
    14881 tagtcatcgc aaacaacgct tctatcagtg gtgcagaagg gggatgtcaa gcagaagttg
    14941 gttctgcaag tgcaatggca gcagcagctt tggtttgtgc aaaaaatggg actcctcatc
    15001 aagcagcaca agcagtagcc attacattga aaaatatgat gggactgatt tgcgatcccg
    15061 ttgcaggctt agttgaagtc ccgtgtgtca aacgtaatgc tttaggctct tctcaagctt
    15121 ttatctctgc tgatatggca ttagcgggta tcgaaagtgt cattccgccg gatgaagtcg
    15181 ttgctgcaat gtaccaagtc ggacgtcaaa tgccgtcgat tttcaaagaa acagcagaag
    15241 gtggcttagc tgttacgcca actgctaaac gacttgcgga agagatttta aataagaggg
    15301 cgtgataaag ctccttaatt tcgagtatta agagattagt tatcttcata acaaaaaacg
    15361 aagagaggac taattgccct tctcttcgtt ttttattaat ccatcaattt cgtatttttt
    15421 acttcatcga tcgtttttgt tcctgcaagt tgcatcgtga tcgacaattc tttgttcagg
    15481 tgttcaaaaa cagatgttac tccttctgcc ccacctagat tcaacccata aatcactggt
    15541 cggccaatcg caactaagtc agcaccactt gccagagctt tgaagacatg ctctcctcga
    15601 cggacaccag agtcgaaaat cactggtaca cgtttgttga ctacttcggc aatttttggc
    15661 aatacttcaa aggacgcagg acctccatct agttgacgtc caccatgatt ggatacccag
    15721 ataccatctg cccctgcaga gatagcgatt actgcatctt ctggcgattg tattcctttt
    15781 acgataactg gaagatgggt gatttctttg attgttttga tgtcatctgg tgtcaagcct
    15841 tgttttgcag ctgcataaat ttctgcgatt ccttttcctt cgccattacc acttgcactt
    15901 tgttcgctat acgcagctaa gttaggcatt ggtaatggaa attggaattg attgatgacg
    15961 tcttcttcac ggtaaccacc gagcgttgaa tctgccgtta aaatgatagc tttagcccct
    16021 gcttctactg ctttgttcaa aatgaactca ttgaatctgt cgtctttact catatataat
    16081 tggaaaaact gcggtgcgcc tggcgctgca tcggctgcgt ctttgattgt cgtatttgca
    16141 taagtgctga tggaaaagat tgatccagca gcagctactc ctttagctgt atcagcttcc
    16201 cctttttcat gtgccaatcc ttgggccgct gatggcgctt ggataatcgg tgtatctaat
    16261 tcaattccga aaacagatgt atgcaaatcg gctgagtcga ttccgcgtaa tatccgtggc
    16321 atgattttct ttgtattaaa agaagtcgta ttttctttca tcgtccactc atcttcagaa
    16381 ccaccgcgaa tatagccaaa agctcctgct tccatgcgct cttttacacg accttctagt
    16441 taggcaatgt ttacaatatc gattggatgt tcttcattac ttgtatgata agtcatagta
    16501 gatccttctt tcatcttctt gattacatat aaatcatact aaacttacaa aaagtaagca
    16561 aacatttact ctcagatact tttatcgcta ttctttccaa ataaatcgac ttacaagctg
    16621 atctacttct tggttttcat gaagcatact atgagaggca ttttcacctg tcactacttt
    16681 ttcctctatt tcatttccgc gttgcttcaa caacgcatag accgctaatg cactgtttaa
    16741 cggtactgtt ccatcactca agtctgtttc atctagttga ccagcaatca agaaaaatcg
    16801 agtggttata ggaatattac cgatcatttg ctggtaatct tgatagcggt tagaaacgac
    16861 agcaggcccc tttgctaact catctgtcag agactgcgca ctatcatcga caaaatcatt
    16921 aaaaggcgcg ccaattgctg ttaatgtctg aacttgtggc aatgacgtat cctgtccata
    16981 cgttcctaaa tagcgtagtg cactaactcc tcccatggaa tgccccacta tgttgacttt
    17041 atctatgccg tagttttctt tcagatacaa aagacaagtt ttgatccatt ctgcttgatt
    17101 ccactcgtta tttttattat cttcaaacaa tacttggacc atcggattgt ctgcttttcc
    17161 agacaaatct cctgttgctt gtatttgtcc atctgttcct actgttaaaa ccaattcttt
    17221 tactgcttgc tgattttttt ccaaacgccg caacatccct ttaaaggaat tttctgtccc
    17281 gctgtagccg tggaaaaaaa gtgttggaat agtggttttt tctatcactt tactggattc
    17341 tgccgttttt tctttatcag aaacttcagt agtatttatc ttcgtcgtct ggcaaccact
    17401 taacgcaaac aaactgataa ggactaaagc tgctgcagtt gcacgtttgc ataaattttt
    17461 gattggttct gtcatacacc tcttcctttc catatagaaa aaagaaagac ttgaaaggcc
    17521 aatcatatga tcgacatttc aagtctcttt attaatcgat tttatctact tgatcgaaat
    17581 caagttctgt actggtttca cgtccaaaca tatcgatgtt gactttcaat ttttcttttt
    17641 ctccgtcaac ttctgttact tgtccttcta atccagcaaa tgcgccttcg atgattttca
    17701 cgacttcgcc aacttctact tctagatcat tttgtcgcag gctcataccg atagaacgca
    17761 agatatgatt gacttcttct ggaagtaatg gtgctggttt gcttccggct ccgtgtgaac
    17821 caacgaatcc tgtcacacca ggtgtgttgc ggacaacata ccatgaagcg tctgtcatga
    17881 tcatttcaac aagtacgtat cctgggaatg ttttatgaac gatttctttt tctttcccat
    17941 ttttgacttc agtttctttt tcttcaggaa caacgacacg gaaaataaag tcggccattt
    18001 tcatactttg cgcacgagat tcgatatttg cttttacttt gttttcatat cctgaatatg
    18061 tatgcaagac ataccagttt ttttcaaaag attccattga aggctcctca tttcattgta
    18121 aaataaaaaa acctcagtac acccgaagtt tttctttcgc ttcattatag cactaaaagt
    18181 acgttgtggc tagcgagtta ttttaaaatc cagccaaata agctttggat ggctgtatcc
    18241 attacgtaaa acatcaatcc gaaaataata gaagtttcga tgactactaa cgtatcttta
    18301 cggagttgtt ttctgcttgg ccaagttact tgtttgattt catcttttac gctttttaga
    18361 aatttcatga gacaacctcc cttgctactg tttatttcgt ttctttatgt aatgtatatt
    18421 gattacaata tttacaaaac ttattgattt ccaaacgttc gccgcttttt ccttcactga
    18481 ctgattttgt gtagttgcga gacccacaaa cggaacaagc gagtgctgct tttttctttg
    18541 ccatttactt ctgccccctt gctgagaatc agacttatac ttcataacag tatcacgtaa
    18601 ctttttactt gtcaatttgt gaaataaact atctattgtt ttggataaaa ttatgatatg
    18661 attaatttga aattaagcta aaatgagaca attggaggta tagcgatgct attaaaaaca
    18721 cttgtatata agaaaaaaga tctgacaacc gttacagaga ctagtacatt agaagaagca
    18781 ttaaaaattt tagaaacttc cggataccgt tgtgtgccga tcttagattc ttcaggtaat
    18841 attttccgtg gaaacattta caaaatgcat atctatcgtc ataaagcaaa tggcggtgat
    18901 atgacgcttc ctgtcactca tcttttgaaa aacgctacaa agttcattta cctggatact
    18961 tctttcttca aagtattctt tacgatcaaa gagcttcctt atattgctgt ccttgacagt
    19021 gaaaatcatt tttatggtat tttgacacat agtacgttgc ttaatatgct ctctcaatct
    19081 tggagcgtag aacaaggaag ctatgtactg acaattgctt ccacaggaaa gcaaggagat
    19141 ttggctgcta ttgcaaaaat catttcaaaa cacagcagca ttgccagctg tattactctt
    19201 gatatcgaaa aagacgaata cattcgacgt acattgatca cgctaccggc taacactgaa
    19261 gcgaaagtat gtgaaacgat cgtcaaccac ttagagaaaa agaattataa agtggtagaa
    19321 attgaaaatt tgcaaaacca agaagcataa tcaaaaagcg aaaaaagaaa acagctaatc
    19381 tgctttcttt tttcgctttt tataattaga cacgcttttc aaaatcaagc gcgcgatcta
    19441 aataatacat caatggtgct gaaacagcca tgacgataaa ctcacttaat gcaagcgtgc
    19501 cataggttgt ccaaaatgct tggccacctg ttggaactaa catataagca atcagtccca
    19561 tgctgatggt aaagaagatc gtgttcaata ctaaacgcat ctttacattt ttcactttgt
    19621 tttgcaataa agctgtcagg cctaaagaaa gcaatgtttg gccgccacca aaaaccacat
    19681 ccatgatacc aaaaccaaag aaggcattat agacgatgac cccgcctaaa actccccaaa
    19741 gcaatttacg gttaaagaca actaaatgat tcaaactttc tgaaatacga aactggatcg
    19801 gccctgagga tacaggcgca actaaaatag ttaacgctag ataaagagcc atgatgatgc
    19861 catttacaac gattacggat aaccgttgtc tgcttttcgt tttttgcatt ttctctcctc
    19921 ttttctccta gtttttttaa cgcgggatgg ttgatgaacc gcggttatgt gaaacaaaca
    19981 ataattataa cagacttttc atttatttca atccgtctta tctgctttta aaaaagcaaa
    20041 agcttaaaca atgtaaacgt ttgctaaaaa tcgtgggata tggtataaag taagtgtaag
    20101 gggtcataag aattcccagt aaagcagacg aagtaggatc tgcaagaaca gaattgttgt
    20161 gaaacttaaa taaataaaaa aggatgtggg aatttgagca acctcttact agagaacggt
    20221 ctagcaagca gctgaagatc taagtggaca tgccgaacct tggaacttct ccttacgtaa
    20281 gtaatggatc atgcgcttgc taggcttttt tgtgtttttc ggtcttcgga cctttttcat
    20341 tttcctagac ttctgctata ataaccaaga attaattttg taacagattt ttttgaaaaa
    20401 acaagtttct ttcattcaaa aaatagaaaa agcttgctaa ttattggtta accagcaaac
    20461 aggagtatta aaatatcttg ttttattgaa tagcaggagg aaatcatgaa attccattat
    20521 catatattga tcaatccatc ggcaggcagc ggaaatggct ataaagtagc agaaagaatt
    20581 ttaccggtat taaaaaataa gcatattgac tatacacttc attatagtga atacaaagga
    20641 cacgatgctg agattgctga aactttggca aaagaaacat tgattccttg gatagaagaa
    20701 gaacagaaaa caatcgatac tttccccctt ttgatcatcg taggaggtga cggcacctta
    20761 catcaggtac ttgatacttt ctatcagatg gaagtagaat tcccagttgc ttatattcca
    20821 gcaggttctg gagatgattt tgctcgaggg gcaggccttc caaaaaatcc caaaaaagga
    20881 cttcagctga ttttagcagc tcaatcacca gaaaaagtcc acataatggc ttatgaagaa
    20941 aaaataagtg aaaaaaaagg aatagctgtt aataatttcg gtataggatt agatgcagct
    21001 atcgtccatg cgacaaatca ttcttccaca aaaaagcgat tgaacaaata caacttagga
    21061 tcattttcgt atttattctc tattctccgt gctttattca ctcaaaaagg atttccaatt
    21121 ctggtagatg caggaggaaa acaattaagt ttttcaaacg catttttatg tacagcaacg
    21181 aagcatcctt tttttggcgg aggaatcgcg attgttccaa ctgctgatgc gtctaaacca
    21241 gtcattgatt ttgtcgtggt cgaacgaatc aatttcttta aaatcttgtg gctcattctt
    21301 ttacttatac agaaaaaaca attgaaatcg aaatatttcc atcactacac aacaaacaga
    21361 ttacgtatcg tttcaacaac accgcaacat ggtcaagaag atggagaaga aatggaaaaa
    21421 cgaccttttg atatcacaat aaccacaact aaacagttgt tttggtgtta ataaaagagg
    21481 ttttgtcaga agctacatct ggaacaccgt ttattcggaa aaaaggactg tgacacgact
    21541 tttgtcacag tccttttttt actatgcagt taattctttg cccaattggg ttgccatcga
    21601 tagtttcttt ctttacccaa aataaattgg atcattgaaa acaccgcagc aaaagcaact
    21661 aagacacaac cattgaacca aaaagattct gcaaatgttt gattgtaact gagtgtttca
    21721 ctgatcaagt tattattttg aacaaaccaa tatgttggtg tgaatgccgc tattttgcta
    21781 accatttctg gcaagtattc tgctggtaca aacaccccac cgataaagca actgcctgtg
    21841 ataaaaatat ctttgatccc accgctggtt tctgagtttt ttaccaatga agtgatacaa
    21901 gccgaaaagc taatgatcgg caataaaaac aagaaagtat ttaaaatatg ataccatacc
    21961 gttatatccc acgaggtgtg gatcactaac agaaccatta gaagtaagaa gccactgaac
    22021 aacagcagcg agtaactaat caaactaaag gttactttgc gattcaagtt tcgtttggta
    22081 atgggcgcac tctggtttcg tttactgatc tctttgcgat taaatgctag attgatgacg
    22141 ctgaaaccgc taaaaatgga tagaaacaac ccataagaca agaaattaaa ggttgtgccc
    22201 gtcaacagct ttttcatccg ctgactatat cccgcactta gctcaacttc tccagtaacc
    22261 gagagtgcca tttgtgtctg ttccatagct gcatcgattg ttttatcttc ttcttgaaac
    22321 agaatcagtg tttgcaaaaa ggtgttcact tgctgtgtaa caactgtttt agaaaaggca
    22381 tctgggcgac tctgtatctt gatcgtcggc atctttccta cttcgacttg ttcggtaaag
    22441 tctgcaggca gatgtaagat aatgtttaca cgattaaaat aaagagcatc atcgatttct
    22501 ttttgtgagg ttccatccaa tgtaacgact gtttccttgt cattcaaata tttacgaagg
    22561 ccatcgacta caggcgactc ttcttcacta atcaaggcga ttttcgcatc ttgcagagag
    22621 gtattttcaa catcaatatt ttgcgcaaaa aagaatgtgg caacaacagt gataattaca
    22681 cctatcagta aattcgattt atttgcttta agcaatgtcc aaatcgtttt ataaagcgtc
    22741 ataacgcacc cctctttcca tccaagcatt tcctaacgca aaaaggaaaa taaatccgat
    22801 caaccataga agattggtat aaaaactagc caaatcagtg tagaaataaa gttt
//
LOCUS       DACDSY010000029        22373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDSY010000029 DACDSY010000000
VERSION     DACDSY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.029"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            592..1920
                     /locus_tag="IWK44_000969"
     CDS             592..1920
                     /locus_tag="IWK44_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9549512.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="IWK44_000970"
     CDS             2122..2955
                     /locus_tag="IWK44_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP9549513.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="IWK44_000971"
     CDS             2971..3708
                     /locus_tag="IWK44_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP9549514.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4789)
                     /locus_tag="IWK44_000972"
     CDS             complement(3863..4789)
                     /locus_tag="IWK44_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP9549515.1"
                     /translation="MKENMLTGGVSPDVANPLLDAEITIPKHVELLVEDIFNLPSPHI
                     MPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIGNKLPIVLTGAMRSS
                     NELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTKTHTTNVATFRTPTL
                     GPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGDLLEAIAHTKVDGLV
                     IEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDYEGGGKELEKMGIIF
                     CNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5064..5897
                     /locus_tag="IWK44_000973"
     CDS             5064..5897
                     /locus_tag="IWK44_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP9549516.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5931..6770
                     /locus_tag="IWK44_000974"
     CDS             5931..6770
                     /locus_tag="IWK44_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9549517.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6796..7356
                     /locus_tag="IWK44_000975"
     CDS             6796..7356
                     /locus_tag="IWK44_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9549518.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7555..9276
                     /locus_tag="IWK44_000976"
     CDS             7555..9276
                     /locus_tag="IWK44_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP9549519.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9441..10583
                     /locus_tag="IWK44_000977"
     CDS             9441..10583
                     /locus_tag="IWK44_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9549520.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10599..11810
                     /gene="thiI"
                     /locus_tag="IWK44_000978"
     CDS             10599..11810
                     /gene="thiI"
                     /locus_tag="IWK44_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP9549521.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12383..13030
                     /locus_tag="IWK44_000979"
     CDS             12383..13030
                     /locus_tag="IWK44_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9549522.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            13423..16068
                     /locus_tag="IWK44_000980"
     CDS             13423..16068
                     /locus_tag="IWK44_000980"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP9549523.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16378..17691
                     /locus_tag="IWK44_000981"
     CDS             16378..17691
                     /locus_tag="IWK44_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP9549524.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17681..18334
                     /locus_tag="IWK44_000982"
     CDS             17681..18334
                     /locus_tag="IWK44_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9549525.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18389..18940
                     /gene="radC"
                     /locus_tag="IWK44_000983"
                     /pseudo
     CDS             18389..18940
                     /gene="radC"
                     /locus_tag="IWK44_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            19071..20156
                     /locus_tag="IWK44_000984"
     CDS             19071..20156
                     /locus_tag="IWK44_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9549526.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            20153..22045
                     /locus_tag="IWK44_000985"
     CDS             20153..22045
                     /locus_tag="IWK44_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9549527.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            22052..>22373
                     /locus_tag="IWK44_000986"
     CDS             22052..>22373
                     /locus_tag="IWK44_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9549528.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKEN"
BASE COUNT         7684 a         3639 c         4687 g         6363 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt attttttttc attttacaga cttccttacg aataattttg
     4861 catagccgtt aaatcttatt gtatactata taaaagagtt ttgctatcag tgaagaataa
     4921 cgaatagcct aacttatcat aaaaaagctg accttattaa aatggaaaag actcaacctt
     4981 gacctcctaa tacaatggag tatctggttg agtttctttt acaagaaagc agccaaggaa
     5041 atcaatcacg aaggtggccg gaaatgatca gaatcggatt aacatcgttt aaagaacatg
     5101 acaaattaac tggtaaaaag aataacactc tttatgaata tgcagggttt ctacccttag
     5161 tggaattaga tactgcctat tatggaattc cccgaaagtc ctcagttgag aattggataa
     5221 gtgaagtacc tgaatcattt cgctttgtag tcaaagtcta tcaaggaatc agcggtcaag
     5281 gagactggaa gccttattat cagaccgaag aagagatgat tgcgcatttt ttagatgtta
     5341 tgtcgccaat ggtagatgct ggaaagatgt tttgcttttt agtccaattc ccctcatcat
     5401 tcaaatgtac gaaagaaaat gtccagtact tgcgcaaact tagaaagtgg tttgagggtt
     5461 atcctgtttc tatagaattg cgggattatt cttggtacag cgatgaattt cttgaacgta
     5521 caagaaactt tatgtctaga gccgcttttt ctttagccat catcgatgag ccacagttgc
     5581 tgaatacaac gattcctttt gatccatttg tcaccaatcc gaactttgct ctttttcgat
     5641 tccatggtag gaatgcgcaa ggatggcaag caaatgataa agagtggcgt aaaaaaagaa
     5701 cgttatatcg ttattcagaa aaagaactgc aagaactagc acatgaagtc aaagtagttg
     5761 attctaagac aaaagaggta ggtgtgattt ttaataataa ttctggtggt gatgcggctg
     5821 aaaatgcatt agcactgcaa aaaatattaa atatcaagcc agatggactc aatccaaccc
     5881 aaatggatct gttttaaggg cttattttat tcaagaaagg ggcgaagtct atgatcaaag
     5941 ctattttttt tgatattgac ggaacattgg tcaccaatcg ttcaaaagcg ttggaatcaa
     6001 caaaacaagc aattgaatat gctcggaaaa acggcatttt atgcggtgta gcaacggggc
     6061 gcagcccagt aaagatcaaa gaaatcattg atgaattgga attagatatg tatgtcgtgt
     6121 ataatggtca actggttttt actgcagatc gcacgattat cgaccaccct tttgaacaaa
     6181 aagtattgga acatattgtt gaatttgcag atgagaacca tcgacaaatc gtttttggtg
     6241 cgagaaatcg gttagatgga tcaacgacga tgctattagg acaatcgatt tttatcaagc
     6301 gactagtcag ctttttaccg cgtaaattcc cggtacgtct gatgaaaaaa atcctccagg
     6361 tttttagccc tcaccgacaa aaggatcgtt atgaaacgtt agagatatta aaagaaccaa
     6421 tttatcaatg tatattgctc agtccggagt cagaacagaa aaagttagaa catcgctttc
     6481 ccgagtgtac gtttcagcgt tcaaatacgt atactgtaga tatcatccca aaaggtggtt
     6541 caaaattact aggcatccaa gctttcgcaa atgctgtcga aattgaaatg gaagaaatta
     6601 tggcattcgg cgatcattac aatgatatcg agatgctgaa aggtgtagga attggagtgg
     6661 ccatggggaa tgctcagatc gaagtaaaac atgctgcaga ctttgtgact caatcaaatg
     6721 aagaagatgg gatatacgaa ggactgaaac acttcggtct gatttattag tcaatgaaag
     6781 aaggaaaaaa tcagtatgga agcagagaca ccttttcaaa aagtagaaac attccacaaa
     6841 acttttgatc caagaagacc agagataccc acggctttta cagaaaatga agctcataca
     6901 cgtgctgcgt tcaaaataga agaacttgtt gaatttgttg cagctacttc acctaataaa
     6961 gaacaactgc atgaagctgt tatgctgctg cacgaagcat tagataaagc agaagaaaaa
     7021 atacttggta aaacacagtg gggaagtgat ccgctggttg aacaagtaga tgcactgaca
     7081 gatatccttt actttactta tggttctttt tcattactag gaatagtgcc tgatcgaata
     7141 ttcgaaatcg tccatcaggc aaatatgggg aaattatttc cagatggaaa acctcattat
     7201 gaccctgtaa caaataaagt gttgaaacct gaaaactggc agatggaata tgctccagaa
     7261 cctaaaatcg cggcagaaat ccaacgtcag attcaagaag cgattgctaa gaagaaatcc
     7321 gctgatatag gcatgtgcac aaatgaaaaa gagtgatcag agtttcacta tttttgttat
     7381 ctttatacga tggaaaaaca aaagattttc gtttaagtta gtgaatgcat gaaaatagta
     7441 tgctataatc tagaatagat gtcttttgca cgagagacgt attatttgga tttatgccaa
     7501 gcagaacgcc atctgttgca taaaagatgc atactgaaaa cggaggaacc ataaatggga
     7561 aataatgtta tcatcggaat cgtcattgca atcattatag tcgccattgt cgtctttttg
     7621 gttagttact tgatgaagaa aaaaaaccaa gatcgattgg atgccttaga aaaacgcaag
     7681 gaagaattat ttgacttgcc agtgattgaa gaagtcgatg atgttaaaaa aatgcatctg
     7741 gttgggcaaa gccaaaatac ctttcgtgaa tggagtcaaa aatggaatga catatctact
     7801 tcctcgttcg ctgaactaga aagtcaaatt ttcgaagtgg aaaacctcaa tgaaagtttt
     7861 cggttcttaa aaggaaaaaa agcagttgtt gaagcagaag ctacaatgga ccaaatggag
     7921 aaacaagtga cggaaatccg tgaaggtcta aaggaattgc gggaaacgga agaacgcaac
     7981 tcactagctg ttcagcaggc acttgacgtc tatgaagaac tgaaagaaaa agtcagagaa
     8041 gacgagcatg aatacggacc agctcttttt gaattgcaaa aacagatcaa agtgatcgaa
     8101 aaagaattta cgcagtttgt gactttaaac acttctggag accctgttga agcccgcgag
     8161 gttttggata aagcagaaaa acatacgtat gaagtggaag ctctgatgaa aaaaattcca
     8221 ccgctgtatg aggagctcca taccactttc cctgaacaac ttgaagaaat cagtgatact
     8281 tacgagaaat tgatggaaga acaatatgta ttcccagaag aagatcttgc tgaagatatc
     8341 gcaaaagtaa gtcgaagaat tgaaaattca ttagccaatc ttgaaaaaac agaagttgaa
     8401 acggtcgaat ttgaaaaccg tgaaacagct gatttgattg attcgttata tgatatctta
     8461 gaacgggaaa tggaagctca gagatatgta aaaactaacc aatcaacgat tgcagagtat
     8521 atcaaacata caacaaagaa taaccgtcaa ttattgattg agctggatca cacatctcaa
     8581 agctatacat tgaaccacaa tgaaattggc cgtgtacgag gtttccaaac cgaagtggaa
     8641 gaaatggaac gtcaaaatga acagatgatt ccacagatcc gtcaacacga aattccttat
     8701 tcagaagtgc gcacgttcta taaaacagtc ttcaaagtct tagaagatat cgaaaagcaa
     8761 caagtagaga tcgacgactc attacatgaa ttgagaaaag gcgaaaaaga agcgcaagaa
     8821 aagatcgata cattcgaatt caaattgcgc agtttgaaac gttttgtaga aaaacaacgt
     8881 ttgcctggct tgccgaatga ttacctagaa ttcttttttg ttgctactga ccgtattgaa
     8941 gaactaagtg ttgtcttgaa taaaattcga gtaaacatgg aagaggtcaa tcgtttagtt
     9001 gctctatgtg aagaagatct agaattatta gataagaaga cacatgattt agtagatgca
     9061 gcagcattaa cggagcaaat gctccaatat gcaaatcgtt atcgtcatac acacgaagaa
     9121 gtgagagaag caattgataa aagctactat ttattcaata aagaatatca ctatcaagaa
     9181 gcattagacg aaatcggtac agcattagag cgagtagaac ctggtgcttt caaacgcatc
     9241 gaagattttt attttaatca tccagactta gtataaagca aaaaagttag taaaccagca
     9301 agagcaagac ggattattgt cttgttcttg tctttttttg tatatttgaa aattgaaaca
     9361 agtgaaaatt tcacctgctc ttgaaagcac cttggacaac cgttataata gaaaagaatt
     9421 tcaaaaaaga ggggtttctt atgatatatt ttgataacag tgcaacaacc ccaatctttc
     9481 cgcaaagctt agatgcatat gtaaaaacca gtcagcggat tattgggaat ccatcaagct
     9541 tgcatgattt aggcaatcaa gctaaccggt tgttgcagca agcaagaaag cagatcgctg
     9601 atttgatcca tgtagaacca gaagaaatct attttacttc aggcggaaca gaaggagata
     9661 actgggtatt aaaaggaacg atgatcgaaa aaagagaata tgggaatcat atgatcattt
     9721 caggagtcga acatccggcc gtcagcgaga cagcagaaca attaaaagaa ttaggaatcg
     9781 aagtaagtat cgccccagtg gataaacgcg gatttgtacg tgtcgacgaa ttgaaagaat
     9841 taataagaaa agagacagta ttagtctctg ttatggctgt gaataatgaa gtaggatcga
     9901 ttcagcccat ccaggaaatc agtgatcttt tggatgattt tcctaaaatt catttccatg
     9961 tagatgctgt tcaggcgatt ggaaaagtac cattctcgca atggttgaca gaaagagttg
    10021 attttgcaac attttcagct cataaattcc atggcccacg gggaactggt ttcatttatt
    10081 ggaaaaaggg tcgtcgttta gcaccattac tgacaggtgg agggcaagaa agaaatcagc
    10141 gaagtgggac agaaaatgtt ccaggtattg tggctatggt taaagcttta cgtttatcat
    10201 tagagataaa ggaacaaaaa cctgagcaga cacaacgctt aaaagtagct ctaatcaatg
    10261 cgttgaagac gtatgagaaa gtgacgattt tttcagaagg aactgactat gcaccgcata
    10321 ttttatgttt tgctctaaaa ggaattcgcg gcgaagttct cgtccatgcc ttagaagaaa
    10381 aacaaatatt tgtttctact actagtgcct gctcctcacg taaacatatg gcaagcagta
    10441 cattgcacgc aatgcatgta ccaaatgatc tggctacatc tgctgttcga atcagcttag
    10501 atgaaaacaa ttcgcttgca gaagtcgagc aatttatgat tattttcaag cagctttata
    10561 agaaattttc tgcaatcaat taaaagagga gtgttcttgt gaaatacagt gaaattatgg
    10621 tccgttatgg ggaactttcg acaaaaggaa agaatcgaaa aagctttatc atgcaactag
    10681 cacaaaatgt cagacaagct ttgaaagact ttccagaaat caaaatccat gctgatcggg
    10741 accggatgca tcttttattg aatggcgcag acgggcagct agttattcca aaactagcaa
    10801 aaatatttgg gatccaaaat ttctctccaa gtattcgtgt agaaaaagat gtcgaagttt
    10861 tgaaacaagc tgttcaagac atcatgaaag aaatttatac tgggaaagaa acatttaaaa
    10921 ttatcgctaa aagaagcgat caccaatttg aattgacaag taatgaattg aatcaaacat
    10981 taggtaatgc tgtttttgat attttcccgc atatccaagt tcaaatgcgt caaccagata
    11041 ttccattacg tgtagagatt cggcgtgatg gcgcttatct atcgtatgaa acgatcaagg
    11101 gagctggagg attgcccgta ggaacaagcg gaaaaggaat gcttatgctt tctgggggga
    11161 ttgattctcc tgtagcaggg tactttgcca tgaagcgggg agtggaaatt gaagctgttc
    11221 attttgctag tccgccgtat acaagtgaac aagcactgca aaaagcaaaa gatctaacag
    11281 ccaaactagc accttatgtg ggaacgatcc attttatcga agtgccattt accgagattc
    11341 aagaagaaat caaaaagtca gtgccacaag gatattggat gacaattact cgtcgaatga
    11401 tgttgcgttt gaccgatgaa attcgccgta ttcgtcatgg tttagtcatt ttgaatggtg
    11461 aatcattagg tcaagtggct tcacaaactt tgcaaagtat ggtggctatc aatgaagtaa
    11521 cgaatacgcc aatcattcgg cctgtagcga caatggataa acttgaaatc attgaagtag
    11581 cagaacagat cgatacattt aatctttcta tccagccttt tgaagattgc tgtacgattt
    11641 ttgctcctcc acagccaaaa acacgccctc aactggaaaa ggtccatcaa tatgaagagc
    11701 gtttagcaat agatgaaatg atccaacgag cactagctgg cttgaagatg gaagagatac
    11761 aagcagaatc gtctaatgaa aaaactgaag aattttcaga attgctataa gaggttatga
    11821 aaatagtgtt ttgaccagag tattagagaa acaaaacgaa aaaatagctc catgtatttt
    11881 accgtttttg aactaatgtc gaagattagc tgattgaata ccatttatca agaagttatg
    11941 gaaaagccgg ttctttttta ctaactgaaa aataaaattt tatttagaga tcaagctaca
    12001 gttgataaat gatataaaaa agagaatttc ccatgaaata gcgaggttgt gacagaagtg
    12061 ggcagcttca agaaataaga aggaatttac gaaaattgct tttcaaattt ttgtgaaatt
    12121 tcggcttatt tttcgaagaa gttacttctg gaacaccgtg tatcagaggt tgcgaaaaag
    12181 ctgccctgca tcaagtaata agaacagcca aacaaaaata gttcctcata ttttacgttt
    12241 ggttgagctt attaccgcag atgcaagctt ttgaacaccg ttttatttgt ctcacaccct
    12301 tttccttcct acttgtaacc ttgctcccgt gttagaaaca tgttacacta aatatgtgaa
    12361 attattaaca aggagcgtcg ttatgcgaga aaaaacagat acactgccaa aagcaactgc
    12421 gaaaagatta cctttatatt tacgctactt gaagatgctg gatgactcag gtataagtag
    12481 aatcaaatca aatgagttca gcgaaatcac ccaaattcct tctgcaacga tccgcagaga
    12541 cttctcgcaa ttaggcgagc taggcagaag cggatatggc tatgatgtcc cttttcttat
    12601 cgatgtattc aataatatcc ttaatactaa agaagaaaaa cgaatcgcat tagtaggata
    12661 tggaaatctt ggcaaagcat tgaaacacaa caattttaga agaaatgaaa atctaaatat
    12721 cgtgtgcgtc tttgataatg atccagcact gatcaatcga gtaattgatg gagaaatgat
    12781 ctatccgatt gatcgtttcg cggagatagc caaagcaaaa aatgtgacag tagcaatctc
    12841 tacggtacca agtaaatatt cacaatctgc tattgatgaa attgtcaaag gaaatgtaac
    12901 cgctatactt aattttgcgc ctgacagagt aactgtccca gcttatgtca atgttcaata
    12961 cattgatttg acaaccgagt tgcagacgct tatttacttt gatgagaatt actcagaagt
    13021 tttttcttag gaaaatttga gtaggcttat tgatcttgcg agaactcgtc gtaattcttt
    13081 caaaaatttt ttagtactaa atgaagtggt tttttcacga ccacttgttg actaaaaaaa
    13141 aaaaccattg tagaatgaag cataacgctg aacaagagga gtaactggca cattcattct
    13201 tagagagaga actatctgct gaaaggttct caatgacaac cagtgaaggt agcttgggag
    13261 tttctgtctt gaatggagta gagatagacg agtcgtgatc gttaactcac ttgagaggca
    13321 ataatgattg attattgcaa tttaggtggt accgcgtaaa tcatacgtcc tagaatacga
    13381 aagtatttta ggacgttttt tagtttaaag gaggaaaaca gcatgacgga agaaaataac
    13441 ctatcaacaa aatataatcc gcaagaagtt gaagctggac gttatcagca ttggttagaa
    13501 caagatttgt ttaaaccgag cggagataaa aaagcaaaac cttattcaat cgtaatcccg
    13561 cccccaaatg ttactggaaa acttcattta gggcatgctt gggatacgac cttgcaagat
    13621 atgctgattc gtcaaaagag aatgcaagga tatgatactt tatggctgcc tggtatggac
    13681 catgcaggaa tcgcgacaca agcaaaagta gaagcgaagc ttagagagca agggatttct
    13741 cgttatgatc taggtcgtga aaagttcatc gatcaagtgt gggaatggaa agaagagtat
    13801 gcgagccaca tccgcgaaca gtgggctaaa atgggcttat ctttagatta cagtagggaa
    13861 cgttttacat tagatgatgg attatctgaa gcagtacgta aagtttttgt taccttatac
    13921 gaaaaaggtt tgatttatcg tggtgaatat atcatcaact gggatcctca agcacgaaca
    13981 gctctatctg atatcgaagt tattcataaa gatatcgaag gcgcctttta tcatatgagc
    14041 tatgaactag cagatggatc aggcgtagta gaaattgcga caacccgtcc agaaacgatg
    14101 ttgggagata cggcgattgc tgttcatcca gaagacgagc gttatcaagc gctcatcggc
    14161 aaaaaagtga ttctaccttt agtcaataaa caaatcccaa tcattgcaga tgaatatgta
    14221 gatatggaat tcgggacagg tgtagtaaaa atcactccag cacatgatcc aaacgacttt
    14281 gaggtaggta atcgtcatga tcttccacgc gtaaatgtta tggatgatga tggtacgatg
    14341 aatgaactag caggaaaata tgctggaatg gaacgttttg ctgctcgtaa agcaatcgtg
    14401 caagatctga aagaaatcgg tcgattgatt aaaatcgata cgatgatcca cagtgtgggt
    14461 cattccgagc ggacaggtgt cgttgttgaa cctcggctgt ctactcaatg gtttgtaaaa
    14521 atggcgccgt tagcgaaaaa agccattgat aaccaagaaa cagatcaagc agtggaattc
    14581 ttccctccaa gattcaatca aacgttttta cgttggatgg aaaatgtcca tgattgggta
    14641 atctcacgac aattatggtg gggacatcaa attcctgcat ggtatcacaa agaaacaggt
    14701 gaaatgtatg tagggatgga agcaccagcg gatagtgaaa actgggtaca agatcctgat
    14761 gttttagaca cttggtttag ttcagctttg tggcctttct caacaatggg ctggccagat
    14821 acggaaagcg aagattataa gcgatacttc cctactagca ctttagtaac cggttatgac
    14881 atcattttct tctgggtaag cagaatgatt ttccaaagct tggaatttac tggcgaacgt
    14941 ccatttgaga atgtcttgat tcatggattg attcgtgacg aacaagggcg gaagatgagt
    15001 aaatcattag gtaatggaat cgatccgatg gaagtaatcg aaaagtatgg agcagatgct
    15061 ctccgctggt tcttatcgaa tggttctgca ccaggacaag atgtccgctt cagctatgaa
    15121 aaaatggatg cttcttggaa ctttatcaac aaaatctgga atgcttcacg tttcgttatc
    15181 atgaatatcg aaggaatgac cgtagaagag atcgatttat ctggagaaaa atcagtggct
    15241 gaccggtgga tccttactcg tttgaatgaa acgatcgaac gagtaactga gctatttgat
    15301 cgttttgaat ttggtgaagc aggtcgacaa ctgtataact ttatctggga tgatttttgt
    15361 gactggtaca tcgaaatgag taaagaagta ctttatggag atgatgctgt ttcaaaacaa
    15421 accacccgta gcatcttagt ccatacactt gatcagatcc tacgtttatt gcatccaatc
    15481 atgccattcg ttacagaaga aatatgggaa catattcctc atcagggaaa ttctctagta
    15541 gtggcagaat atcctgttgt acgtccagag tttgatgatg aaacagcttc taaagggatg
    15601 gaagtattga aagaattgat tcgagccgtt cgaaatattc gttcagaagt gaatacacca
    15661 ttgtctaaac caattacgtt gctaattaaa acaaacgatg ctaaaatcga tcaattttta
    15721 gttgaaaata caagctatat cgaacgcttc tgtaatcccg aagagttgac gatatcaagt
    15781 gagatcactg cgccagatct agcgatgtca gctgttctga caggtgcaga aatcttcttg
    15841 ccgttagcag gattgatcaa tattgaagaa gaaatcaaac gattagaaaa agaactggaa
    15901 aagtggacaa gcgaagtaaa acgtgtccaa ggaaaacttt ctaatgaacg ttttgtttct
    15961 aacgcgccgg aagaagtagt agaggcagag cgtgcaaaag aaaaagatta tttagagaag
    16021 caaacggctg tcaaagaacg aatcgaaagt ttgcgtacta ttcaataaga atagaaaaag
    16081 agagtgtgaa atcagctagc tattactaaa agctagctga ttgaacatcg ttacaggaaa
    16141 gagtttgagc ctcactctta gcttcgattc ataaggagat gtgacataaa ttttgtcgca
    16201 tctctttttt agtctactct tattatgtag gagaaggagg gaggtggatc atgtatcaag
    16261 agtcaataaa gtagttgata aaatcgttcc tctctctgtt tcttttctct ttgtcatact
    16321 tttatttttc ttatgtgttc tatttgttat aataaaagag aaatggggga attgttgatg
    16381 acgattgaag aagcaataga atggatccat agtcgattac catttggttc acgtcctggc
    16441 cttgaccgag tcgaagcttt attagaagtg gtagggcatc cagagagaaa agtaaaaacg
    16501 atccatatcg ctggaacaaa cggaaaaggt tctactgtag cttatttgag aagcctatta
    16561 gaagaggctg ggctgacagt aggtacattt acttcacctt atatcgaaac gttcaatgaa
    16621 cggatcgcaa tcaatggcaa aagcattcca gacaatgatt tgatccagct tgtaaaaaaa
    16681 tatcagccac tgatcaaaca acttgatcaa tttgaagcag tagctggtat tactgaattc
    16741 gagacattaa cagcaatggc atttgattat tttttgatga agaaagtgga cgtagcgatt
    16801 atcgaagttg gcttaggcgg cttattggat tctaccaatg tagctgtacc aatgctgacg
    16861 ggaataacaa cgattggact tgatcatacc gaaatacttg gagatacaat tgaagaaatc
    16921 gctgcacaaa aagctggaat catcaaacaa ggcgttccag tagtaacagg aaatattgcc
    16981 ccagaagctt ttactgtcat ccaacaaaca gctaaagcaa aaaatgcacc gctttatgta
    17041 tacggggaaa attataaaat aaactatctg catcctgatg agatgtgggg agaagtattt
    17101 gaatttttta atgaaatagg aaagatccct caattaacca ctccgctttt agggcaacat
    17161 caagctgaaa atgcaggagt agccatcgaa ttgtattttg tttactgtca attggaaaag
    17221 ctgcctttcc gagaaaaaga tgttttgaag ggattgaaga aatctcgatg gccaggaaga
    17281 atggagcgtt tgagtaacga gccattagtt gttttagatg gtgcccataa tacgcatgca
    17341 atgaaccggc ttgttgagaa tttgaaaaaa gaattcaaag gatatcaaat caacattttg
    17401 ttttccgcct tacgaactaa aaatgttaga gaaatgctag aacgattgca gcaagtacca
    17461 aatgtccata tttatttgac gacctttgaa catccaaaag ccatcgaatt aagtgaagta
    17521 tcagatttag caagtgagag agtatcaatc gtttcattgt ggcaattcgg attagcagat
    17581 atattggaaa agatgacaaa tgaagacgta ttagttgtga ccggttcact ttatttcatt
    17641 tcagaagttc gccatttatt aatacaatta ggaggagatc atgaagtata atgcagtgat
    17701 ttttgacatg gatggtttgc tgttcgacac agagttagtg tattacgaag ccacacaagt
    17761 agtagcagac aagatgggtt ttccttatga taaagacctg tatttaaaat tcttaggtgt
    17821 ttcagatgaa gaagtatggg cgaattatca tatgattttc tcggaatttg gcaaaacaag
    17881 tgtacaaaga ttcatagatg attcatacca ggaaacgctt gaacgttttt cgcaaggagc
    17941 ggtacaaatg aagcccggtg tgttggaact cctctctttt ttagaagaac atcagattcc
    18001 caaagttgtc gcttccagta accaacgagc agtcattgaa cttttattga agaagaacaa
    18061 tctgtttgaa cgatttgaga cgattgtttc tgcagaaaat gttcaacgag caaaaccaga
    18121 cccagaaatt ttcttgttag ctaatcagaa gctaggtaca gcgaagaaag aaacattgat
    18181 tttagaagat tcacaaaatg gtattttagc agcttctgcg gctgagatcc cggtagtcat
    18241 ggtaccagat ctgcttccac catcccaaga attgatcaaa aaaacagctg gggttgtttc
    18301 ttctttacat gaaatccctg cattgttaaa ataaaaatcc atctcttttc gtatcttatt
    18361 ttagataaga tagaaaggag atgttttttt gggaaaacga ttgatcaaag aagtacctac
    18421 tagctcttta ccaagggaac gtatggaaat ttatggcgcg gaagctttat cggatcagga
    18481 gcttttggca atcttactgc gtaccggtca acacccttat agtgtgatgt cgattgctgg
    18541 aaatctgtta aaaacgtttg gaggactggc ctctttacga caggctactt tgcatgagtt
    18601 agaagagatt cgagggatag gacgcgtgaa agcaatcgaa atcaaagcct tgatcgaatt
    18661 aggtcgacga attcagacgg atcatgaaag agcggctcca ccggttcgta ccagctacga
    18721 attggcccag caattgattc tggaaatgaa agatcacaaa caagaacatt tggtttgtat
    18781 ttatttggac acgaagaatc aagtgatctt aaaaaaaaca gtgttcatcg gttcgctgaa
    18841 tcagtcagtc gctcatccta gagaaatttt ccattatgct gtacgttatt gtgcagcaag
    18901 gatcgtttta gcgcataatc atccttctgg taatgtttta accatagtat aataaataat
    18961 agtagttaaa gtagtttaag aggagatatt aaagggatat tttactttaa ataaaaatta
    19021 acaaggttta taatttgtag tagttaaagt atttaaagag gtgggaacat gtgaaggttc
    19081 aaaggataga agtggagaat aagccgtatc cattgtattt attactagat aaagaatacc
    19141 agctaataga accagtaatg aaatttatta aatacttaga taatactggt aagtctccta
    19201 ataccattaa agcatactgc tatcatttaa agttgctgta cgagttcatg gaacagagag
    19261 gtgttattct taatgatatt aactttgagt tgttagcaga cttcgtaggt tggttgagat
    19321 atccttcagc atcaaatgta attgatcttc agtcaaaaaa agccataaga gaagaaacga
    19381 cagtgaatac aattttaaat gtagttatga gttttcttga ttatttaagt agattaggag
    19441 aatttaaatc aattgatgta tttaaacaag caaagggaag aaatttcaaa ggatttttac
    19501 atcatgttaa taagggtaga taccaaaaga atgtcttaaa gttaagggtt aaaaagaaac
    19561 agataagaac attgagatca gaggaagtta agcaaattat tgatgcttgt catacgaaaa
    19621 gagataaatt aattttaatg cttatgtatg agggtggttt aagaatcggt gaagtgttat
    19681 cgcttaggct tgaagatatt gtcacttggg acaatcaaat ccatttaaca cctagagatg
    19741 ttaatgttaa tgaagcttat attaaattaa ggaaggaaag aacaatacat gtgagtaaag
    19801 aacttatgtc actttataca gattacttga tatatgagta tagtgaggaa ttggagcatg
    19861 attatgtttt tatttcctta aaagaaggct attttgggaa accactaaag taccaaagtg
    19921 ttcttgatct agttagaaga atagttaaaa ggactggaat agaatttaca tcacatatgc
    19981 ttcgccacac tcacgcaacg cagctaatta gggaaggatg ggatgttgcg ttcgttcaaa
    20041 agagattagg tcacgcacat gttcagacaa cgttaaatac ctatgttcat ctttcagatc
    20101 aggatatgaa aaatgagttt aataaatacc tcgagagaaa ggagcataag aaatgaatgc
    20161 ttctagtaaa aggaaaatta ttagtcagag tgagattagc aaaaaaatag ctgtaatgaa
    20221 tgaagaaatg caggggtttt gggctaataa tagttgggat ataagaaaat gtccacatcc
    20281 ttctgccata gaattaagta agaatcctgc tttaaggaat cgttgggttc gttttgaacg
    20341 tgttaaaaat ctgtggttaa gaacagaatt gaaatatttt tatttttacc atttaaacaa
    20401 tggaatatgg aatgcaaaaa ctgtctggat tagaaaagga acagtaatta ataaaatgtt
    20461 agatttctta gatttaaagt atcctagcat tacttcaatt actgaagttc ctattgaaaa
    20521 agcaatgacg gagtatagaa cttatttgac aaaacggggt gttagaatta ccactactaa
    20581 ttataagatt actgctaatc aagaaaaaac acctgtaaaa gctaattcct actatgttac
    20641 taatctaaaa caatttatgg agttttatga gaacttttat tttgatggag aggagtggga
    20701 taaagacgtt tgggatagac gtaacttacc tttgccagat gataaggtta acccaacaca
    20761 atatgaatat acaattaact ttaaagggtt tcggaataca tattttaaac aacttgtaaa
    20821 aagatattgt aagttgagat tgaacatgga tagcttttcc tatgtaagtg atattgccca
    20881 aagacttaaa gagttcttta attttctgga catgaaattt aaacaagttc agagagtaca
    20941 ccaattaacg agagtggaaa ttgaagcata tttaagtgaa ctaaacatga tgggaataaa
    21001 acctagtaca ataactggga ggatctctat attggaagga ctatttagta cccttcttag
    21061 gctagaatgg gatgatgttc cttccaaaat attaatttat tctgaggact atccgaaaat
    21121 accaagagca aaaccacgct ttatagatga attcgtccta gagcaattga acagtcatct
    21181 tgataaatta cccgaatata tagctacgat gactatgatt gttcaagaat gtggaatgag
    21241 gataagtgaa ttgtgcacct tgaaaaaagg ctgtctatta gaggacaaag atggagattt
    21301 ctttttaaag tattatcaat ggaaaatgaa aaaggagcat atagttccaa tatctaaaga
    21361 ggtagcttta cttattaaag ttcgggaaga taaagtttca gaggaatttc cagatagtga
    21421 atacctcttt ccaagaaaag atggatcgcc attaaaacaa gaaacattta gaggtgagtt
    21481 aaataaatta gcttatgagc aaaatatagt ggataaatca ggtgagattt atagattcca
    21541 tgcccatgcc tttcgccata cagtaggaac aagaatgatt aacaacggga tgccccagca
    21601 tattgtgcag aaatttttgg ggcatgaaag cccagaaatg acaagcagat acgctcatat
    21661 ctttgatgaa actctaaaaa atgaatttac taaatttcag gaaaaactgg ttaccaataa
    21721 tggagatgtg cttgatctag atgaagataa tgaagtcgat gatgtagagc ttcaatggtt
    21781 caagaaaaat ataaatgcac aagtgcttcc aaatggttat tgtagattgc cagtagtagc
    21841 aggtggttgt ccacatgcga atgcatgctt agattgcact cacttctgta ccagtaagca
    21901 attcttacca cagcacgaag aacagttaga gcgtacagaa gagttattag ccatagctaa
    21961 ggataaacaa tggcaaagac aagtagagac taatagccgt gttaaagagc gtttagaaca
    22021 aatcattgga agtttgacgg ggtaattatc aatggataaa caagttagaa atacaacaga
    22081 aattgtacgt ttggcgaagc agaaatcaaa aaagacaagg gaaaaagtag acaaagcgat
    22141 ttctaaattt tcgattgaag gtaaagttat taattttaat tcaatagcaa aggaagctaa
    22201 tgtttctaaa tcatggcttt ataaggaaca cgatattagg caaagaatcg aatcccttcg
    22261 tgagcgtcaa ataacagcaa atgtagtctc aaaacccaag aaaagttctc gttcggagga
    22321 aatccttatt aaaaccttaa aaagaagagt aatggaatta gaaaaagaaa ata
//
LOCUS       DACDSY010000030        22203 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDSY010000030 DACDSY010000000
VERSION     DACDSY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.030"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            14..256
                     /gene="yaaA"
                     /locus_tag="IWK44_000987"
     CDS             14..256
                     /gene="yaaA"
                     /locus_tag="IWK44_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP9549529.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            243..1367
                     /gene="recF"
                     /locus_tag="IWK44_000988"
     CDS             243..1367
                     /gene="recF"
                     /locus_tag="IWK44_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP9549530.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1364..3310
                     /gene="gyrB"
                     /locus_tag="IWK44_000989"
     CDS             1364..3310
                     /gene="gyrB"
                     /locus_tag="IWK44_000989"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP9549531.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3330..5801
                     /gene="gyrA"
                     /locus_tag="IWK44_000990"
     CDS             3330..5801
                     /gene="gyrA"
                     /locus_tag="IWK44_000990"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP9549532.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6037..6336
                     /locus_tag="IWK44_000991"
     CDS             6037..6336
                     /locus_tag="IWK44_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP9549533.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6386..6898
                     /gene="ssb"
                     /locus_tag="IWK44_000992"
     CDS             6386..6898
                     /gene="ssb"
                     /locus_tag="IWK44_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9549534.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6924..7160
                     /gene="rpsR"
                     /locus_tag="IWK44_000993"
     CDS             6924..7160
                     /gene="rpsR"
                     /locus_tag="IWK44_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP9549535.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7303..9276
                     /locus_tag="IWK44_000994"
     CDS             7303..9276
                     /locus_tag="IWK44_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP9549536.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9282..9734
                     /gene="rplI"
                     /locus_tag="IWK44_000995"
     CDS             9282..9734
                     /gene="rplI"
                     /locus_tag="IWK44_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP9549537.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10008..11375
                     /gene="dnaB"
                     /locus_tag="IWK44_000996"
     CDS             10008..11375
                     /gene="dnaB"
                     /locus_tag="IWK44_000996"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP9549538.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12155..13447
                     /locus_tag="IWK44_000997"
     CDS             12155..13447
                     /locus_tag="IWK44_000997"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP9549539.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            14183..15046
                     /locus_tag="IWK44_000998"
     CDS             14183..15046
                     /locus_tag="IWK44_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9549540.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(15175..16797)
                     /locus_tag="IWK44_000999"
     CDS             complement(15175..16797)
                     /locus_tag="IWK44_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9549541.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            17046..17567
                     /locus_tag="IWK44_001000"
     CDS             17046..17567
                     /locus_tag="IWK44_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549542.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(17753..19483)
                     /gene="spxB"
                     /locus_tag="IWK44_001001"
     CDS             complement(17753..19483)
                     /gene="spxB"
                     /locus_tag="IWK44_001001"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP9549543.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            19648..20466
                     /locus_tag="IWK44_001002"
     CDS             19648..20466
                     /locus_tag="IWK44_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP9549544.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(20569..20766)
                     /locus_tag="IWK44_001003"
     CDS             complement(20569..20766)
                     /locus_tag="IWK44_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549545.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            20935..21513
                     /locus_tag="IWK44_001004"
     CDS             20935..21513
                     /locus_tag="IWK44_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9549546.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT         7126 a         4010 c         4680 g         6387 t
ORIGIN      
        1 tgagggatgg gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca
       61 aatgttgaaa gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga
      121 aaacactgtg ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg
      181 catgatggtt gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga
      241 taatgaggct gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg
      301 aattttccaa gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt
      361 tggaaagtat ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat
      421 tgattggctg gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca
      481 ctcttccatt agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg
      541 aacagaaaaa gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag
      601 atctttcttt agtaaaaggc agcccgcaga ccaggcggaa gtttttagat atggaattag
      661 gccagatcaa tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa
      721 gaaaccagta cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata
      781 ttttgtccga acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg
      841 tcaagcgatt ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc
      901 agctggaaat cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa
      961 tccaagaaca atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa
     1021 caacgacagt tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc
     1081 agacttatgg atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa
     1141 tcgatttgat taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga
     1201 gcgagctgga tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa
     1261 cgttcttaac aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga
     1321 ttttttatgt tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag
     1381 tttagtagaa cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga
     1441 agccgttcgt aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca
     1501 ccttgtttgg gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa
     1561 aatccatgtg attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat
     1621 acctgtagat atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt
     1681 acatgctgga ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt
     1741 aggatcttct gttgttaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg
     1801 aaatgtgtac tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga
     1861 acaaacggat cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga
     1921 gacaactgag tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa
     1981 ccgcgggatg cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata
     2041 ccattatgac ggcgggatta aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat
     2101 ttttccagaa cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat
     2161 gcaatataca gacggctacc attccaatat catgagtttt gccaacaata tccatacgta
     2221 tgaaggcgga acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta
     2281 tgcccgaaaa caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg
     2341 agaaggattg acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac
     2401 aaaaacaaaa ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca
     2461 tttcatgaaa ttcttgctgg aaaacccaag tgtgggccgt caaattgttg aaaaaggatt
     2521 actagcttcc agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg
     2581 tgctttagag attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga
     2641 aaaatgcgaa ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg
     2701 taatcgagag tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc
     2761 aagcatggac aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac
     2821 agggtttgga gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac
     2881 tgatgccgat gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta
     2941 catgcgtcct attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt
     3001 aaaacaaggg aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa
     3061 acaagtcgtt gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact
     3121 tggagaaatg gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat
     3181 ggcccgcgtc agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg
     3241 ggatcgagta gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt
     3301 agatatttaa tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg
     3361 atgtcaatct aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg
     3421 tcgcacgtgc acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat
     3481 acggcatgaa cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg
     3541 tcggagatgt catgggtaaa taccatcctc atggagacta tgcgatctat gagtcaatgg
     3601 tacggatggc acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg
     3661 gttctgttga cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa
     3721 tcgctacaga aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg
     3781 atgatacaga aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg
     3841 gaacaacggg gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg
     3901 tagtagcggc tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg
     3961 aagtattacc tggaccagat tttccaacag gtggtttggt tatgggaaaa tcaggaatcc
     4021 gaagagcgta tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg
     4081 agatgccaaa tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag
     4141 caaaattgat cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag
     4201 atttgcggga cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg
     4261 ccagcgcatc tgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg
     4321 gattcaatat gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaattc
     4381 ttgaaaacta tatcgaacat cagcgcgaag tcatcacaag acggacagtc tttgaaaaag
     4441 aaaaagcaga agcaagggca cacattttag aaggtttgcg tattgctttg gatcatatcg
     4501 atgaaatcat tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa gcgacgatga
     4561 tcgaacggtt cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc
     4621 gattgaccgg tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga
     4681 ttgaagattt aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg
     4741 aattagcaga cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg
     4801 aggtcttaag tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga
     4861 caaacaatgg ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg
     4921 gacgtggtgt ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt
     4981 gttctaccca tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag
     5041 gatacgaaat tccagaatac gggcgcacag ccaaaggaat accaattatc aatttactag
     5101 gtatcgattc gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag
     5161 gccactatct cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt
     5221 ttgctaacat ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg
     5281 tcaatgtgct actgacagat ggtcaatcta atatcattat tggaacacat aacggctact
     5341 ctgtcacttt caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggttagag
     5401 gaatacgcct gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaagatcaag
     5461 aagtattagt gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag
     5521 tgaaaggccg cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac
     5581 tagctggatt gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg
     5641 tcattatccg tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac
     5701 gtttgatgaa gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag
     5761 ctgcagaaca aacagttgaa gaacaaggat cagaagaata gccatcaaga ctaattgtgt
     5821 aagctagcaa gaatgaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt
     5881 tgaatttctc ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag
     5941 tgtataatca gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa
     6001 cctaagagcc gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc
     6061 atgtacatta ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc
     6121 gatgcaatct tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc
     6181 cgtttcgctt atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct
     6241 tctccatcat cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat
     6301 atcattcgtc acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag
     6361 aaaacgatga gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac
     6421 gaaagatcca gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc
     6481 tgtgaatcgt aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt
     6541 gatttggcgc aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg
     6601 tgttactgga cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt
     6661 gacagaagta gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg
     6721 ccaacaaagc ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa
     6781 taactctaat caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc
     6841 tgacccattc ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac
     6901 gaatttgata ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta
     6961 aagtagacta cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac
     7021 taaaaagatt tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg
     7081 ctaaaaacca acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac
     7141 ctttcgttag cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct
     7201 attcaattcc tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa
     7261 agttatggta aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat
     7321 catttatccc cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc
     7381 ttaattttac catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt
     7441 gttcgctatt ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag
     7501 caggcttcga ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga
     7561 gtgattgctt atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa
     7621 atcaaagatc aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa
     7681 gaaaaaaaga aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca
     7741 gtaaagggtg ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag
     7801 acgaacagtc agccggtgat tggtatttta tctattgata actatgcaga tgccattgat
     7861 aaattggatg acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac
     7921 tggatgaatg aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta
     7981 gcacagatga tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt
     8041 cgagcagaag ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc
     8101 tcaagaaccc tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc
     8161 cgtggaggcg accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat
     8221 ggggggaaat ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc
     8281 gctttgcgtc ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg
     8341 gatatggatg ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa
     8401 aaagcttaca tcgttatcaa cgagaacgag atcattccag atgtagaacg gtgtcttgaa
     8461 gaaatccata agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag
     8521 ctgaaaaaag aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc
     8581 tctcagggcc tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg
     8641 gaagagtttc caagtaaacc tctgctgact tatatcgaat cttcagcttc ttcagcttca
     8701 gagctagttt ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt
     8761 gaggccacat tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca
     8821 actgcacgaa cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta
     8881 gtacaatatt tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct
     8941 ggaagcgact atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat
     9001 aatagtgtta tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca
     9061 gctttcgtca ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc
     9121 acgatcaacg tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca
     9181 gctactcaac tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc
     9241 aaagaaaata tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac
     9301 aagatgtaaa aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac
     9361 aaaattatct aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt
     9421 tagcaggaca aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa
     9481 agttaaaaga attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg
     9541 aagacagccg tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac
     9601 aatacaaaat caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag
     9661 gctacacaaa cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg
     9721 tcgtacaaga ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa
     9781 aaaagacaaa ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa
     9841 atcgatttca gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac
     9901 gagtataata aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct
     9961 ctcattttct tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt
    10021 ggcaggatcg aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt
    10081 ttcttgacgc agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc
    10141 gtcgtggcca tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga
    10201 ttgacgtggt tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg
    10261 gtttaagtta tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact
    10321 atgcgaaaat cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa
    10381 tcgttacgaa aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac
    10441 gcagtatctt ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag
    10501 tactgaactc cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag
    10561 gtctaccaac tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac
    10621 taatcattct tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc
    10681 aaaatgtagg aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag
    10741 aatctcttgt aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta
    10801 caggtcagct ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc
    10861 gagcaaatat ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat
    10921 gtcggaaact agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt
    10981 tgattgaagg taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac
    11041 tgaaaaaact ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg
    11101 gagtagaaca gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa
    11161 ttgaacaaga tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag
    11221 gcggagatga cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg
    11281 agaagaaccg aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata
    11341 agttttcgtc gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa
    11401 tcccgagagc tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa
    11461 tttaaacaga tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat
    11521 aaacggtgtt caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa
    11581 ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt
    11641 ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga
    11701 gaagcagttt tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc
    11761 tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat
    11821 atgagaagct atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa
    11881 cctctgattc acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa
    11941 aatttgagaa gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta
    12001 caacctcttt tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata
    12061 aaataaaaat aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg
    12121 ttcaggatta aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac
    12181 gcaatggggc gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt
    12241 gattgcacgg taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac
    12301 ttataaactg catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg
    12361 aaacggcgtt gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa
    12421 aggtgtagaa acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca
    12481 tatcaaattg gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat
    12541 taaaggaatc ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga
    12601 cttattagat aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg
    12661 tcaatttgta aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata
    12721 ttatgaatac ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga
    12781 tgctttggat gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat
    12841 cgaccaggga acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat
    12901 cggaagcggt gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac
    12961 gtcacgtgtc ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat
    13021 ccgcgaagta ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt
    13081 tgattctgta gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa
    13141 ctcgatcgat gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga
    13201 tggcgaacta atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat
    13261 ctatgaagaa ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact
    13321 gccagaaaac gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc
    13381 aacattttct gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca
    13441 aatctaagga taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt
    13501 ttatctatac gataataata acttgaacac tgtttattca gaataaggga ctgtgacaaa
    13561 aaccgtgtca cagtcccttt ttccgaacaa acggtgttca aaagcttgca tctacggcac
    13621 taagcccaat cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg
    13681 atgcagagca gctttttcac aacctctcca ctaaacggtg ttctcctgcc aactcctcgg
    13741 tcacaagtca caatattcaa caaacatgag aagctgtttt ttgaaattgt tccttgtgac
    13801 tcggagctaa acggcaggct cacaacctct tggtaaacgg tgttcaatca gctgaccctc
    13861 ggtattagct caaaaaacgg caaaatatga ggaactattt tttccgtttt tttctctaat
    13921 actcaggtca aaacgctgat ttcacaacct ctgattcacg gtgttccaga agtagcttct
    13981 acggaaataa gccgaaattt cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta
    14041 tttcttgaag ctgaccactt ctgtcacaac ctcttatttt gattagaact tgcgaaaata
    14101 aaccggttaa agtgtgtcta gcgcaagaga tttgctataa tagacgataa gggtaaaatg
    14161 taacgagaag gggttgaaaa atgtgtatca aattgtaaca gattcttgtt gtgatctgcc
    14221 ataccatgta ttagcaaaag aaaaagttga ttttttatca atgaatctcc aattgaatga
    14281 tcaagagtta gtagatgacc tcggaaagac attcaattat gaccgctaca tgaatcagct
    14341 gaaaaatggt gcgatgccta cgacatcaca aatcaatgtt ggccgatatt tagaattttt
    14401 ccgttcttat gcggaaaaac agattcctgt tttatatgtg gctttttcca gtggattaag
    14461 cggctcttac agtagtgcgc ttcaagcggt tgaattgctg aaggaagaat atgaagaccc
    14521 tgagatctat gtttttgata caaaagctgc aagtattggg gaagggcttt tagtattgga
    14581 agctgtaaga ttgaaaaaag aaggtaaaag catcgaagaa accctagagt ggctggaaag
    14641 taattatttg aaagtccatt catgggtaac agtagacgat ctgaaatatc tagaacgagg
    14701 cggtaggatt tccaaagcaa gtgctactct tggcagtcta ttgagtgtaa agcctattat
    14761 tacggtggat aaagaaggaa ggctgacgaa tgttgacaaa gttcgaggaa gaaacaagtc
    14821 gatccagaca atcgtcaatg agacggtaaa aacaatcgtt gcaccacttg atcaaatcgt
    14881 ttatgtggct tacgctgggg atctagagag cgcggagaag gcaaaacggc ttttggaaga
    14941 gcagataaag atgaaagatg tgaagcttta tcccttagga ccaacgatcg ccagtcacac
    15001 aggctatggc tgtatcgcga ttttttcgat gggaatcagt cgataaaagc agtaactcat
    15061 tctgtaaaaa agcccagaaa ttgattgtaa caaaaacagc gactcttaaa agatagatga
    15121 gcatcctatc tcttagaggt cgctgttttt ttatttttta agctaaatag gccattatgc
    15181 ccataactcg ttcaattgtt tttgaatatc tgcattttcc aaaaattcat catatgtcgt
    15241 atcagctcga tcgatcacac ctttatccga aacggcaatg atgcggtttg ccaatgtttg
    15301 gataaattga tggtcatgtg acgcaaataa gattgagcca gtaaatgcca tcaagccgtc
    15361 attcaatgct gtgattgatt ctaagtctaa atggttcgtt ggatcatcta atacaagtac
    15421 gttagctttc gaaagcatca tttttgaaag catgcaacgg actttctctc caccagataa
    15481 aacattcact ggtttcaata cttcttcacc agaaaacaac attcttccta agaagcttct
    15541 taagaaggtg ttatcatctt cttctttact tgcatattga cgcaaccagt ctaaaatcgt
    15601 tagatcagta tcgaattctt tcgttgtatc ctttggtaaa taagaattac ttgttgtcac
    15661 accccaacgt actgttccag tatctggcgt aatttcaccc atgataactt tgaatagcgt
    15721 tgttgttgtt atatcatttt ctgcgacgaa agcgaccttg tcttctttgc tcaaattaaa
    15781 agatatatca tctaaaattt tctttccatc aatcgttaca cttacatttt ctacctgcaa
    15841 taagtcgttt ccaatctcac gttcaggttt gaatccaacg aatggataac gtctagaaga
    15901 aggttggata tcgtctaaag taattttttc aagcattttt ttacgtgaag ttgcttgttt
    15961 tgattttgat gcattcgcac taaagcgggc aataaattct tggagttctt tgatttgttc
    16021 ttcttttttg gcatttgcgt ttgcttgaag tttagccgct agttgactag attccaacca
    16081 aaaatcataa tttcctacat aaagtttgat tttaccaaaa tctagatccg ccatatgtgt
    16141 acatactttg ttcaaaaagt gacggtcatg ggatacaacg atcacagtat tttcaaaatt
    16201 gatcaaaaat tcttccagcc aattgatcga ttggatatcc agaccgtttg ttggttcgtc
    16261 tagcaaaaga acatcaggtt taccaaatag tgtttgtgct aataacactt ttaccttttg
    16321 tccaccagtt aattcactca tttgctgatg gtgcaattct tcagggatgt tcagaccttg
    16381 cagtaaaaca gctgcttctg gttcagcttc ccatccatta agctcagcaa attctccttc
    16441 taattcagct gctcgaatac catcttcgtc agtgaaatcc tctttcatgt aaatcgcatc
    16501 tttttctttc agcacttcat acagacgctt gtgtcccatg atgactgttt caagtacagt
    16561 atagtcttca tagtcgtaat ggttctgttt caatacggcc atccgttcat ctggtcccat
    16621 agtaacggaa cctgtggaag gctgcagatc ccctgataac actttaagga acgttgattt
    16681 acctgcacca tttgcaccaa tcaagccata gcagtttccg ggggtaaatt taatattcac
    16741 atcatcaaat agtttgcggt ctgaaaagtg caaactcaca tcatttacag taatcaattt
    16801 ttttcctcac ttttatcatt attagttttg acaagtctta tagaattata taggggaaag
    16861 gtaaaagatt caaggcaatc gaacattctt tgtgtttcac gtgaaacaga atgaaaaaaa
    16921 taaaaataaa agcggagcga aaatagctac tataggattt attagtttgc aacagcataa
    16981 tttttgacgt tatttataag agggagtact ataaaactgt aaaagaaatg aaagaaggat
    17041 tgaaaatgga tgaaaaaaga accatcgact ggggctcatt gattttggga attttatttg
    17101 tacttgtctc attgttatca tttcaagacc ctgtcggaaa tttagttgca attgtagtag
    17161 tctttgcgat ttttgccttt attaaaggga tatttgaact gtttgtacgg aacagattaa
    17221 aagagctaac aggatataaa ggaaagatgc ccttaataat cgggatcatc gatattctag
    17281 ttggcgtctt tttcttattc aatattggtg caggagtcgc agcattgcct tttgtttttg
    17341 cagtatggtt tattgctgat tcagttttag cgttatttac tgctgacttg gcaagaggag
    17401 taagtgaagg atattattgg tttacgatca tcgtcaatat tttaggaatt ttattaggaa
    17461 ttttcttatt gtttaatcca atttcttctg cgttgacatt aagcttttta gttggattct
    17521 attttatgtt atttggtatc acacatatcg tctatgcatt tagataagta aaatcaaaca
    17581 aataaaaaag ccggcttccg attacagttt tactgttgta attggaagcc ggctttttta
    17641 tcatggacaa atatgcacga ggtgattttt aggtgtcgag acaaatttat gcaaaaatgt
    17701 gagaagctat atacacaaat tttatcttaa tactcgaagc atagcgcagt tgtcacaacc
    17761 tcttttcata taattcattc aatactggca aatcatggac ttcatatttt tctttgaatg
    17821 cttggatttc ttcttcagaa tattttcttg gatctaaatg cagatcttct actggcaatg
    17881 ggcgcttgtt attgatcttc acatcgatta ctacaggacg atctgatttt gcagctgcat
    17941 caaaggcaaa tcgaagttgc gagtattctg tgactgtaaa gctatttgcc ccgagcgctt
    18001 ctccaacttt accgaaatct gcatcttcta aaaatacacc aaatttcttt tgttccgtat
    18061 cttcttgttc tgcttcgatg aaaccaaaag aatcattcga aaagacaaca ttaataattg
    18121 gaagctgata tttgacttgt gtaatgatat cttgcatgac catagcaaac ccgccatcac
    18181 cacttaaggt aaatacttgt ttttctgggt agcttagttg tgcggcaatt cctcctggca
    18241 caccattacc catagttgcg aaccatccag atgtcgtatg tttttgttta ccgttcatat
    18301 tcaataaacg aatagaatgg atagtcgtgt taccgacatc tgtgacgaaa atcgcatcgt
    18361 cttcagcaat acgattgatt tctttataga cagcctctgg acgaatcggt tcttcctctc
    18421 gatcttcaaa agatttcaac caatttaccc atttttcttt attttcttga tttgctaggt
    18481 accagctatc agcagggcga gcatccccta actcgacaag tctttgcaaa gctgtgtttg
    18541 catcaccgag gatcgacaaa gatgtttcat ggcgtcgtcc aaattttgac gcatcgatat
    18601 cgatttggat aaattcagca gttggattaa aaaatgcacg tccaaatggg aagtcgctac
    18661 cgacaaataa gatcagatcg gcttctgcca atgcttcatt tccgggttta gtagcgacac
    18721 gtccagcaaa accaagaaag ttttcataat catcaggaat aattcctttt gctagaacgg
    18781 aagcgacaac aggaactgaa aaatgttctg agaattcttt gatttgcgga aatccgtttc
    18841 gcgttccttg accaacataa agaactggtt tttttgcttt ttcaagataa ggcaaagcag
    18901 ctagcaaatc tttttcttct ggtaatatca cacttgtttt gtgagtgtga gcggtggaga
    18961 aaggttgttc gtcaatttct tcaaaaccaa gatcaacagg gatagtcacg atagccacgc
    19021 ctttatgctc ataggcggct ttaatggctt catctactac atgcggaaga ctttgtggtg
    19081 tcatgactgt acgattgtat acgctaacat ctgcgaaaat aggattttca tttagttcct
    19141 ggaaagagtt atagttcatg gatgtagaag caacttgtcc taagaacgct actagaggca
    19201 catggtccat ctgtgcatca tacaaaccat tgatcaaatg tgtcgcacca ggtcctgcag
    19261 agccgaagac agcgccgact ttccccgtta attttgcatc tgcagcggct gcgagtgcgc
    19321 cgacttcttc atgacgaact tgaatgtagt tgatttcggt tttttcttga tatagcgcat
    19381 ccataattga attgaaagat cctccgggaa tgccgtaaat atggtcgata ccccagcttt
    19441 caagaacttt taccattgcg attcctgcat tgatttttga catggtgttt cactccttta
    19501 gcaatgatga tacataaatt ttttgtatct agagtataga actttttatc gaaaatccca
    19561 actaattcgt cttttcacaa aaatgtagtc atgtttttgg gaatatgcta gagtagtaca
    19621 tagatagatt caagagaggg gaatggcatg gttgaatggc tattgagact tgaaccttgg
    19681 cagcaagcac ttgctgggac agcttttact tattttatga cagctcttgg tgccgggttg
    19741 gtttttttct ttaaagaaat caaaaaagaa gtgctaaact tgatgttagg atttgcttct
    19801 ggtgtcatga ttgctgcaag tttttggtct ttgttagatc cggctattac aaaagcagaa
    19861 gaaaatggag atattgcttg gctagttgta agtattgggt ttggtttagg cggtgtgttt
    19921 ttatatatgg cagataaaac actgccccat atgcattttg gaccacaaca tgaagcagaa
    19981 ggattgccta cccatttaaa acggacgatc ttgcttgttt tttctattac attgcataac
    20041 attcctgaag gtttagccgt gggggtagca ttcggtgcag cagcaactgc ggataatccg
    20101 acagccgcta tattagccgc tgtttcggtg gcgctaggta ttggtatcca aaacttccca
    20161 gaaggagcag ctgtatcaat cccgttgcga caagaaggtc tgagtcgtaa aaaggccttt
    20221 gtttacggac aagcttctgg aatcgttgaa ccaattgctg gagtgattgg agcgctacta
    20281 gtaacgaaag tggagctgct gcttccttat gcacttgctt ttgccgcagg agcaatgatt
    20341 tatgtcgtgg tggaagaatt gatccctgaa gcacagcaga cattgtccag caaacgtcat
    20401 tttgcagttt ttggcgtcat gagtggattt atcattatga tgattttgga tgtggcatta
    20461 ggataaataa aaaaaatata tatgatatat caaatagggc cgcgaactta tatcgcgacc
    20521 ctattttttt agtaagttat atcataatga agtgaatggt atcatacatt aataatagtt
    20581 ttttctatgt gtatttcctt tgatccagcg aacaagccag atgataagtg cgattgggat
    20641 tagaactttg accgcaaacc ataaaagccc gcttaggaca ctgacaaaaa gactgatgac
    20701 gaagctagct gcaattagca ctaaaagaac catgaagata tttaagaaca tatttttttg
    20761 acgcatacaa aaccctcctc ttgttcattc tttactatag aaaaagtata acagggattg
    20821 tttaaaaaca cctccaactg aagactgatt tttacttcat ctacttaaat gatcaaacaa
    20881 ttgctttttc tctggtttat catctaaaat agagcggaaa aggaggagaa tatcatgcct
    20941 aaaaatcgtc ttgaagcatt tacagatgct gtaatcgcga ttatcatgac tattttagtt
    21001 ttagaacttc atgaacctaa cggagacacg tgggaggcgc tcgctgcatt aggttaccga
    21061 ttttttatct atattgtcag ttttatcatt ttagcgattt attggaacaa tcatcatcat
    21121 ttatttcaag ttgtacataa gataaatgga cgggtcttat gggcgaacag tttctttgtt
    21181 tttgcgcttt cgctttttcc gtttgcaact tcatggatca gtgagtacat caatagcctg
    21241 gctccagaaa tgacttatgg ttttgttacc ttgaccgcta atattgcgta ttatatcttg
    21301 gcaaaagaac ttgtcaaagc aaataaaggc cacgagaaag cattgaaggc gatgttgaat
    21361 aattacaaga aatcgtatct atcgattggt ttgaatgtgt taggaattgt cttagggtat
    21421 ttagtagcgc cagtagccgt gctgatcgca aacatcgtga ttctagctgc ttggatcatc
    21481 ccaagtcgta ccatcgaatc acaatatcgg taaaatgatc cgtgtttaaa atttaagaga
    21541 aaaatataga aaaaataaat aaacgatcaa atatccgagt gatactagta ttcaaagagg
    21601 gatgcagatg gatcaaaaaa gaattcatgt aggactgatt actagttttg tcacgatgct
    21661 tatttatttg gatcaaggtc atttgacttg gacagtagtt ggtgtcgtag gatttttgac
    21721 attattcaca gcaataaaaa aataaacatc tttatgtaat ctgatgttct atctatttaa
    21781 gaaaagcatg tttacagcag tatgattctg ttgtaaacgt gtttttattt gtggaaaatt
    21841 taatttttag aaaagtgtat cgaaaaatag atagttagtt tttccacagt ggataacttc
    21901 gtaagagata aaaataaaaa attagtcaaa gaagggcgta tagatttctt ttgataggag
    21961 atatacaaca gaaatgaata aatattaacg tgttttcaaa cagtataaaa cggtgtggtt
    22021 tttggaataa aaaaataaca tatctcattt tacgcttttg ttattttata cttaaaaaat
    22081 aacgacacag aaaataccgt gtctcagatt tctctactac ttctaaaaac tactgttttt
    22141 ttcctttgaa atgccttcaa aaagtaggat caggcagaat atactggcag aatcaaggag
    22201 aag
//
LOCUS       DACDSY010000031        22096 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDSY010000031 DACDSY010000000
VERSION     DACDSY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.031"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(147..773)
                     /locus_tag="IWK44_001005"
     CDS             complement(147..773)
                     /locus_tag="IWK44_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549547.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1068..1403)
                     /locus_tag="IWK44_001006"
     CDS             complement(1068..1403)
                     /locus_tag="IWK44_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9549548.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1390..1674)
                     /locus_tag="IWK44_001007"
     CDS             complement(1390..1674)
                     /locus_tag="IWK44_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9549549.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1730..3253)
                     /locus_tag="IWK44_001008"
     CDS             complement(1730..3253)
                     /locus_tag="IWK44_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9549550.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3246..4421)
                     /locus_tag="IWK44_001009"
     CDS             complement(3246..4421)
                     /locus_tag="IWK44_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9549551.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4425..4610)
                     /locus_tag="IWK44_001010"
     CDS             complement(4425..4610)
                     /locus_tag="IWK44_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549552.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4576..6270)
                     /locus_tag="IWK44_001011"
     CDS             complement(4576..6270)
                     /locus_tag="IWK44_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9549553.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6267..6740)
                     /locus_tag="IWK44_001012"
     CDS             complement(6267..6740)
                     /locus_tag="IWK44_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP9549554.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(6809..6964)
                     /locus_tag="IWK44_001013"
     CDS             complement(6809..6964)
                     /locus_tag="IWK44_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP9549555.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7098..7478)
                     /locus_tag="IWK44_001014"
     CDS             complement(7098..7478)
                     /locus_tag="IWK44_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9549556.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7482..7697)
                     /locus_tag="IWK44_001015"
     CDS             complement(7482..7697)
                     /locus_tag="IWK44_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549557.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7700..8107)
                     /locus_tag="IWK44_001016"
     CDS             complement(7700..8107)
                     /locus_tag="IWK44_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP9549558.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8373..9848)
                     /locus_tag="IWK44_001017"
     CDS             complement(8373..9848)
                     /locus_tag="IWK44_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP9549559.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(9838..10686)
                     /locus_tag="IWK44_001018"
     CDS             complement(9838..10686)
                     /locus_tag="IWK44_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP9549560.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10723..11085)
                     /locus_tag="IWK44_001019"
     CDS             complement(10723..11085)
                     /locus_tag="IWK44_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549561.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11385..12009)
                     /locus_tag="IWK44_001020"
                     /pseudo
     CDS             complement(11385..12009)
                     /locus_tag="IWK44_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12006..12317)
                     /locus_tag="IWK44_001021"
     CDS             complement(12006..12317)
                     /locus_tag="IWK44_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549562.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12361..12654)
                     /locus_tag="IWK44_001022"
     CDS             complement(12361..12654)
                     /locus_tag="IWK44_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549563.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            12848..13495
                     /locus_tag="IWK44_001023"
     CDS             12848..13495
                     /locus_tag="IWK44_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9549564.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13556..14701
                     /locus_tag="IWK44_001024"
     CDS             13556..14701
                     /locus_tag="IWK44_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9549565.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(14782..15087)
                     /locus_tag="IWK44_001025"
     CDS             complement(14782..15087)
                     /locus_tag="IWK44_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP9549566.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15112..15543)
                     /gene="ruvX"
                     /locus_tag="IWK44_001026"
     CDS             complement(15112..15543)
                     /gene="ruvX"
                     /locus_tag="IWK44_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP9549567.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15540..15806)
                     /locus_tag="IWK44_001027"
     CDS             complement(15540..15806)
                     /locus_tag="IWK44_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP9549568.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16061..16786)
                     /locus_tag="IWK44_001028"
     CDS             complement(16061..16786)
                     /locus_tag="IWK44_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP9549569.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(17147..17217)
                     /locus_tag="IWK44_001029"
     tRNA            complement(17147..17217)
                     /locus_tag="IWK44_001029"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17183..17185),aa:Gly,seq:ccc)
     gene            17334..18086
                     /locus_tag="IWK44_001030"
     CDS             17334..18086
                     /locus_tag="IWK44_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP9549570.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18102..18581
                     /locus_tag="IWK44_001031"
     CDS             18102..18581
                     /locus_tag="IWK44_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP9549571.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(18832..20412)
                     /locus_tag="IWK44_001032"
     CDS             complement(18832..20412)
                     /locus_tag="IWK44_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP9549572.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20405..21148)
                     /locus_tag="IWK44_001033"
     CDS             complement(20405..21148)
                     /locus_tag="IWK44_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9549573.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(21324..>22096)
                     /locus_tag="IWK44_001034"
     CDS             complement(21324..>22096)
                     /locus_tag="IWK44_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9549574.1"
                     /translation="TRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQFV
                     EFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVINR
                     KITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHYDE
                     ILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENITSI
                     SRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
BASE COUNT         6477 a         4472 c         3576 g         7571 t
ORIGIN      
        1 gaaacccata tcaaagaact ataaaggtta tcaaaacagc gtttatgact aaatgaatta
       61 gagttatcta tcactatttt tcaagcgatt taaaccaaat ggcatacaat atggcataca
      121 aaaacttcaa taagccgtgg gggtttttaa atttcttctt gttctttaaa atcaatgagt
      181 ggagctacat aatttgaatc cactttttca aacgtcactt tttgcatcga cgaatgtttt
      241 ctttcccaag ataacgtaaa tagatttgac tctggactta taaagtctac ttcaccttct
      301 agtattactt tcttatgcaa atattcctct aataaatata gacttgctaa agcatacaac
      361 acattttcca aatttgcttc cttataattt gaagaacgac catgttttac tctgttatat
      421 attttccacc aatctggtgt atcttcaggc cacccatctc caaaaggttt tagtgaagtc
      481 aatgttaaag aattcataac cctaacacca ttatccaaac acattaattc gctagattta
      541 ataacagctt taaaatcagt tatgctttta ttgctactgc tatcaaatcc gcaaagttct
      601 ttgaaaataa tctcacactc cgaaccaatc gctagtagta gtttttgata ttcaatagag
      661 aatgttttga aattattctc atcgagcatc acaaaatctg tggtattaat aaactcattt
      721 tctaaatgta gataatactt ccaataaaaa tttgtaaact ctgctttgtc cattaaatca
      781 cctcttttga attataacaa aaaaaacacg tccacaacag acgtgttctt tgatttctac
      841 acaatcagct tgccaaaacc atccaatcag tcctagcatg cctgtattaa aattgaacgt
      901 tcatcatcta tcatctttct tagtccagca tgtttgcaac cacacctaca attttcagtc
      961 caacaataga tttcgactag ctaagtcttg caaaattcag gcagctaacc cttcatttac
     1021 taccttaatt atactaaata cccacaacgt ttacaagtgt aatcattcta agaaatctgt
     1081 ttagctatga tcgttgtata atctttaaac gccgttcctt cattgattga aataatttta
     1141 taactcttgt ttttccaagc tactttcata tcgtttgtta gttcataatc ttgatcgtat
     1201 cgaataataa acgtcaacgt tccttcaaga atggtaccta cactagcaag cacgtctttt
     1261 agcatttgtg aacgcacatt agcccaacat tcaaatacag tttcttcact atccaccatc
     1321 tgcccatctt catcttttac ccgttttgtc tttttaaact tgactctctc agtcaattca
     1381 tttacattct taatgattgg cataataagc acctctcatt tgttgtacta atgcttgtac
     1441 cgttactggt atccgttcgc tgctggcttc ttgacggttc aaataccaat gttgagtaag
     1501 taatgacacc gccaaatcaa attgctgata attatccata tctttatccg ttgcgctact
     1561 atcaatagca ctttttatat attcgcctgc tgtatcaatc aattgttgaa taaagtcatc
     1621 atcaatagtg tgatcgatcc gcatactttt ttttatgcta tccaaaatta ccattttatc
     1681 tctcctaatg aaaaaaaggc agtgggataa accgccgccc aatatttttc tatacttttg
     1741 gtgttacaac tggcgtaaac tcaatataca ctgaagcatt ttgatcaatt ttcttataat
     1801 cgttgcgaac gatcactgca agtccttgtg agtagtaatc gaatttttcc cattgagtcg
     1861 ttacttgatt tctacgagca acaaagatag attgtgcaat atcccccata atcataggga
     1921 atgttccagc tttaggattg gcaaacaatg tatctgcaat caatactact ggcataccga
     1981 ataatgattt accactaggt gctgtcacat cgggttgtaa aatgtaacgt ccatctgaat
     2041 ctttcaatgt atctaggtgg taatatccgc tttggtttag aattaccatt ttatttaatg
     2101 ctgggtctaa tgccacattg tatagttgtt tcaaatcgtc caacgtagca gccgttttct
     2161 tagtgaatgt ttttaataaa tccatgatat gcttattgtc agtgttttct accaatttcg
     2221 ctaactgatc tttgacctct tgtacaatat tcactgctga atcttccaca acctcatttg
     2281 ataaggcaat cttaccagca cgagtttcta ctttataatc aactgaagta aacatttcag
     2341 cgtcaatatc accaatttca gcaagttcag cttttgttgc taacactgct tgttggttag
     2401 ttgctactgg atatttacct tgtccatttg atactgtttt tactgttgca tattgagcta
     2461 agttataatt tgaacgtttc aaatcaaaga cttcaccgat tacttcttcg ggaacaactg
     2521 ccgctgcatt tacagtagtg actccatcac gcacttcgcc ttgtgaacgg atatattctt
     2581 catacccacg caattctgtt ttttcgttat cgattaaagt tttttccatg ttattactct
     2641 ccttttgtgc tttagaagtg gattctaagc tattgtttgt ttgttttgct tggttgttac
     2701 tcataaacga ttcgtaagaa cgcttattta cttggacatt tgatgaatca taagcgggaa
     2761 cggtcacgac actaatttca ttaagtgctt tgattttatc tattgatcgt tcaattgtgc
     2821 catcttcttt tttgtcgaat gaatctaatc caagcacgaa cccaaaggac atagaatcaa
     2881 caaccccttt tgagatattc tcatacacat catttgaata ggtcgtgtcg gttagctctg
     2941 cttcaaaatg taatcctgta tcatcgacat tcaatttcaa cgtacctgct ttgacactag
     3001 ctagtggctt tgaataatcg tgtccatata ataagaacac atttgataaa tccacctctt
     3061 ttaatgcttc gggtgtaatc acttctacaa agccgcctaa atcttttgac ggttgcccga
     3121 atttcaacgc atagcctgaa atagttttgc cgtctgttgg ttgtttttca ttttctttct
     3181 ctactttggg agaatcggct gttagctcag cttcttctgt cagccgtttt tccttttctt
     3241 cttcattatt caactggtgt aactccttcc tgttctttta attgatattt ctccaacgta
     3301 tctaaatacg taaagttcaa acttgctagt aaacgatcgc caccatccat ggggggtagc
     3361 cccattttcg accgcccctc attaatcgtg agaagtgacc ctttgacctg atccaataca
     3421 ttttttactt ttgtttcagg atcagtctct agtaactgat cggaattaaa gcggatattg
     3481 gtttcaagtt tcgtatctaa ttcactcaca aacacattaa agtaatgaat cagcgtactt
     3541 tggatatact ggagattact ttgaacggtg ctagaatgtt cattttcaac gcctaaacgc
     3601 tcaatcggta caccaaacgc cttggcaatt tgttttgtgt tccaatcatt ggaattgata
     3661 agcttcaaaa catcggtgtt tatttctaat gttttgtagt ccattgtttc atcaagaatg
     3721 atcgttctaa gagcattatc tccactactt gaaccgttgg cttcttcaaa cttttcacga
     3781 attgcgttct ttgctgatcc gtctaaatct gacttatgaa ccttcaaaat acctgaccca
     3841 ctgacaccac gagtaaagaa attgtgcaat gtgcggttgc cagcttgttg aattttcagt
     3901 tcatcacgta acgcataaag tggtggtaat cctgttaaac catcttgggt gaaatatttg
     3961 aagtgcaaaa tatcgctaga cttcacacgt ctttttttat caccaatctg ataagacaac
     4021 gtaccattat ctaattgagt aaccgttaca cttgagttag gtaataagtg aatttcttct
     4081 accttttcac cgttgcgttt aatctctgca aagctattac cgtttagcaa catattgacc
     4141 gccaaagcaa actttaaatg ccaaccatcc atttctgaat taggtttctc gtttagtaac
     4201 ttcaccagtt cattatcagc ttgtggcata ttgtttttga ccaattgaat cggacttgaa
     4261 gcaatatcac tagcaataat tcgaacggct gcaaacacat cactatttct taatgcacga
     4321 acgcttgtaa agctggtggt gtaatcatcc gattggattg ataccacatg ttgtaggaaa
     4381 ggatctcccg ttgtattttg tctcgcttta aaaaatgcca tcttttaacc tcccttctgt
     4441 tctgtattta gcatgaccgc taccccaata agcactatgc ccagcgctaa caagccatag
     4501 tagacatttg ttagtaaggt gatagcaacc aacacacaca ccaatccaag aagtaaaaga
     4561 acggtatgaa tgtaattaga aagaaaattc ttcacttgta taaaattcat tgtctgcttt
     4621 cgctccttcc gttgtggtga agtaatccat tgcaaacaca taggcattga ttaatgcagc
     4681 tattggatca attttgttac tgttctttga tttattaatc tgaatgccgt tgttatccgt
     4741 ttttaaaata gcgttgttca ctgcatgggt gagaatcgta tttttattgt gaataacgtt
     4801 gccttcataa acttgttctc taaaagttcg ggtcggaacg tttagagtaa tcgttccttg
     4861 tcttacttct aacattgggt agttggcttt ttcagcttta ctgataagcg aattagcgtt
     4921 atacgggtca taacaaatcc ccatacattc caaatcattt tcttgaatca aatcttgaat
     4981 aaactgaaat acttggtcat agtccacgat tccactttct aattgagtaa tggaacactc
     5041 accagctttt tctagttctc gataaggcaa accgtcgcgc ttttctttgt cttgaagccc
     5101 atatttggtg gctacaaagc tatgtgaatc acaataaagt tcgccgttat ctagcggtac
     5161 gatccatgaa acgctagtta aatcatctgt tttagataaa tccactccga tataaacggg
     5221 cttgcctgta atatcttgtg gttctacttc ggttgcctgc cagtcttcag caatcatata
     5281 gctgtcctca ctcgcttgtc tccataaatt gaagtttttc accaatacag aattaaggtt
     5341 attttgtttc aaagcaaggt tcacatcatc ttgaatggtt ggaatcatta cttttttgat
     5401 ttcttcactt tcaaaaatcg gattggcttt gatccatttc tcttgatcgt gaatttcttc
     5461 gggatcgtcc aattcccata tcgctataaa gtacctgtcg gcttgttctt cgccttttag
     5521 cacacgatct aacaataagt attcctcgta cataggaacg tttaaatcaa gcccagcggt
     5581 actaataaca cacaataagc cgttcttttg ttgggtcatt cctgacttaa tgacgttgta
     5641 cgttttacga gtttttgctt cgtgccactc gtcaagaatt gcggttgttg gtgcaaaccc
     5701 atctagcgtg ctggtttcac tagccagtgc catagcgaac gaattagagg gcaagtgctg
     5761 aatcttagag ttcataattt tcagttgtgg tcttaaaaac ttactagact tgaccacgtt
     5821 tctaagtgaa ttagacagca tatcatagcc caatttagct tgtttcaaag cgttacttac
     5881 aaacaatact tgccttgctt ctgctggttc ttgttccata atcaacgcat tggcagccat
     5941 gccacttgcg atatacgttt tcccattctt acggctcata ctggtaaacg ctcgattgaa
     6001 tcgcctgtaa ttcccttctt tagtacgcca gccataaaga ctgcctagaa tgaatttttg
     6061 aaatcctaac atcttgattg ctttaccgtc cgtagatggt agcaactcaa tgaatttaat
     6121 cgctttggtg gcttgttctt catcgaacac ataagggaag ctatctgact tagaacgctc
     6181 caaatcgctt atatgacgct cacacgcttg gataattttc ttgggtgcaa tcagcttacc
     6241 tgataggact ttctcaatgt aatcattcat gaattcatca actctttgaa cgggtcttcg
     6301 ggttcatcat ttttaacctt gttcaaagct agttttgatc gtgattctaa ggtcatacct
     6361 agtgaggtgg cttgtttttt caaatcactc atagcttgtg attgaacccg aaccgctgga
     6421 ttggctttct ttactccgtt ttcgcatgtg ataaacgtac ctgattttct tatctcggct
     6481 tcggctgttt tcattcgagc aaaggctaaa caataggcta ccaatgaacc ataatctgct
     6541 tcactcaatg gataatcttt ttttaatgtt ggtacgatac gattccattc acttctagcg
     6601 ctcgctgcca tccagtcagg gggcgtgata tttattaatg gttgctgctt gaacaattct
     6661 tctctagcgt ccacacggat tgctttttct tcacttgata tattgccttt tgtatcgtgt
     6721 aatattttag ggtgtcccat gttgtcctcc tttcagacaa aagaaaaagg aacgtaagca
     6781 attaagctca cattccttcg tttttcgatc agaatttttc tttatctaat ttatctatat
     6841 aaatgatttt tccgttctgc atttgaatgg tcacttgacc aaaatgaggt aactcaatgg
     6901 tgcgctgttt ccctgtttta tcagtaataa caattgtatt tttcaataaa tctcctactt
     6961 tcattatata ctttttcact tgtgattaca agtgtaatcg ttatttgtaa ttacccttat
     7021 gccttttcac aacggttatt tgtaccaaaa tgagtaggca tcgattgtca gtaagtttca
     7081 cactatgccc ccttaactca aagggtactc ttttctcgtc tttgtctttc attggctgtc
     7141 ttggaattat ggtgtgcatg gcataaagaa cgaaaattag tttcatctaa ccgtctattc
     7201 caatcatctt ttagttcaat tttgtgatct acaacatcag ctttccttat caagccttct
     7261 tctaggcaat cctcacatac tggatgattt aaccgataca agtatgaagc ctttcgccat
     7321 gttctacttt gatagaattg aaaatattta ccttcgcttt gctttctttc ttcacgctta
     7381 attgccgttt gctttgcttt atgcttccca caatacttct gcctataatc aatcaatgtt
     7441 ttacatccag catgattaca ttgtttttta actgccatag attaccaacc aaagcgatct
     7501 cgttcgtcag aatcatcttg ttcccatttt gatttgtatt caatataagg cgaaactatc
     7561 ttcaagcacg ttccgtcttt aaaattaact ttagtgattt tatcttcctc aaaactaata
     7621 aagtctacat cttcttgagt gtttgaatac tcaatgtgtt tctcaccatc gaagtatcta
     7681 atactttcca tatgcataat tattctacct ccaataaatt agttatcaat tcatcatctg
     7741 ttagtagcaa agccaagaac ttatcatgga tgtttaaaaa caaattatcc tcattcgatg
     7801 taagctctcg ttgtctcgct cgatcatata ggttaagcag acaattaaat tcatccatta
     7861 gctcacctgt atcatcatag ttgccactat ctaccaatga tcccactact agcgattgac
     7921 accacgctac tgactcctgc agccaatcag gttcttcttc cattatcttt aatacttctg
     7981 gatcaaatgt ggaataatct tctgttacca atcccacact aaatgccagc gtgttcatta
     8041 gaaaccgatc catttcatct gtatattgaa agttcggtgc tttgtttgtt gaaataatat
     8101 tgagcattta ctttcctcct tgattcgtaa cacgtggtaa cacctacgta acaggtaccc
     8161 gttaccactc aatcccaacg gggacaaggg ttctagcgtt ttggtaacag gtaacgggta
     8221 aaatccagta acattattcc ccagcatatt agctaactat ttcaaaagtt ttaaggaaat
     8281 aaggtgttac cccgttacca aattgctgta tcccttgggg cagtaagggt aaactagtaa
     8341 cgggctaggt gttactgacc cgttactacc cgttactttt tgtactttcc agctcgattt
     8401 ttattttcat aggcatttga cttattgtaa aatcctcgtc gtttatttct ttttggcagt
     8461 tgcttccgtt cccaaccagg aatactgcta ataatcaagg caatcttttt agtttctgca
     8521 tctgatcgac cattcaataa atcctttgct tgtttatcaa aaacagcttc caaaatatca
     8581 gcggtgagta cgctatcaac taaataaaac tcttgttgca tagtagctcc attatagaaa
     8641 tgctgtaaat ttttctcatt acatttactg tcaggatagt tctgataata agaacgtctc
     8701 gcccacgctg gagcttcttc ccatttatac gggatttcca tttccaaata tttagtgatt
     8761 gcttctttaa ttggattctc catcttggca tcctcttgat agtcttttgc aatttctaag
     8821 acttctttat cttcatcggg actaaaatag attcgttgtc ctttatcaaa aagcaccttt
     8881 gcttcagcta acacttgaag aaaatattca tcctttgttt taaacggatt catctttggc
     8941 ttattcttac aaggcaaagg ataaaaacgg cggtttcctg tatcatcttt caaatactgc
     9001 ccatcattcg acgttccaat aaatacacat tgccttgccc aagctatgac attccgacca
     9061 taaggaggtc taattttatc gatcttggca ctgatgaagt tttttatctg ttcaatttta
     9121 gttttgttca ttgaagataa ttctccaagt tcaataatta cattgccttg aagctgcata
     9181 taatcatctt tcttttcacc tagcgaatct aatgaatcgg tgaaaaaatc atccgtgtaa
     9241 agtaatgctg gaacggtact cttaccaatc ccttgcggtc catccaaaac tggaacgatc
     9301 tcaaacttta ttcctggttt atagatacgt gcgactgatc cgactatcca acgttttgtt
     9361 acttctcgtg tgtaaaggtt gtcgtctacc cctaaaaggt caataaacag tgtttccagt
     9421 ctaggctgtt gatcccattt aactttttcg atacgttctt ttactgggtt gaaaacattc
     9481 tttctagcca catttactac tgctttgtgt attttgttgt catcaaaaac tacattgtag
     9541 ttttcttcaa tatatgccgt taaggcatcc tcaaaactgt cttcccattc tccagcggta
     9601 cgattgatcc aaggagaatc ttttaatagg tgaatgtatc cgctaaattc gttataagca
     9661 attgttccag ctaaattgct atcattggtt aaaatcaatt ctgcattcac aacactgtta
     9721 cttttaatct gatcgtcttt attgcatctt aatcgtgaca tccaggtttt caattcaatg
     9781 atattgtctt cacgctcgct ggcttcggga aagtctaaaa tactatctaa tttatgctta
     9841 tgagccatga gctttccccc tcaaatcttt tttaatcatc gatcgaataa tttgattcac
     9901 ttctttatct tttagcggtg gttcattgta cgcatttgct gtataaatca attctgctaa
     9961 agtttgatta tctacaccgt gccatagtaa ccaaccgcat aacttagtta aatgatcgtt
    10021 tctactacct tcgccagcgc cttgaacaat catatctaat ttttttgctg tccctgtttt
    10081 tgctacgggt cggtattgag tctttatttc ttgcggtttt tttagcagta ggttcactag
    10141 ccaatcggga gctggtgcaa tattatttag agatacgttt tcaataggtt catatgctct
    10201 cccaacactt cttgaaataa tcatagttgg cgctattgga actcctttag tgataatatc
    10261 aatcccgctt aaacttttac ctttatcatc acgcccgaag atatcagcaa atgcacctgt
    10321 ttcttgttgg ataattacac catcaggaat tttaaagaag aaatggattc ctttacctgg
    10381 tgtgatttcc gtataagtag aaggaaatgg atcatacatt gaagcaagtt ttttataatt
    10441 ctcaacgccg tttcgtgttt cagaatgttg atctaggtca actaccacca tttgatgatc
    10501 ttcaagcatt aacccaatat ttctagttgc atcttccgtc caccattcat taatttgatt
    10561 agaatcgtta gttgcatcat tccaaccact agagcctttt attggaattt tagttccagg
    10621 agataaggga taaattgacc atcccgactt ttgtaattcc gttgctgctt gataattatt
    10681 tgccaataaa cctctcctcc tttttcaaaa tactggtata aatcattcag acacctccat
    10741 gagcttcaac tgctcaagtt cgtatttcaa cccatcccga acaaattcag caccttttaa
    10801 ttgattctga tactttaccc atgtattaaa tgttttcacc cttgcttctt gcatatcagt
    10861 aatgtttaac catgcggtta aaatctgatt ttttagctcg ttgatttctc gacaaattat
    10921 tttgtgttct gcatgttttt tgagtacata caaaactgtt agtttttcat tgaatgaatt
    10981 tccaaagtag gttttcccga tattattttc tagttcagcg atcccttgat taatttcctc
    11041 taattcaagg ctatcgagtt ggtttaatag ttttgttttg tacatctact tatgccctcc
    11101 agttcttaat tgatagtcgt actcgtcata agcagtgaag agatataacg ctaatcctgc
    11161 aaagatagct atttttgcaa ttaatggtaa taagccgaaa attccgccga ttaaaatgct
    11221 tcctataatt aagtttcgtt tcataatggt tccctccgat tttttaatat ggtataatcg
    11281 gaacaaagaa tatcgggttt tatcccaatt aagttgcctt tatcctgtcc gccaagactg
    11341 ttatggataa ggctttttct ttgttctcga tcatgttaat gctcctaagt tgtctgcaat
    11401 tcagcagaac gattataaat tttctcactc actgaaaact gcaacccata tttttcttta
    11461 acagtcatta attctactgt ctcatctaaa ataggttcac gatcaaccaa catttcagga
    11521 gtcatatctt tcttttttac cattttggaa tgaccataaa tagttgatac agctttattg
    11581 gctatcacat tcgctttaat gtagtctact ggttttggtt cagtaatagc gtggcttaat
    11641 ttagtcattg cttctttttg gtgttctttg tctagcatgc ggaatacttg aaagccttca
    11701 agtcctgtgg actggcgtag ttctttgatg atgtcgaaaa tccagtcttg aaatgcctcg
    11761 gcttcttttt tacggctttt gaatgccatg cggtaaatat tttttttcgt tgatgatgtt
    11821 tacatcttgt gttctgccca atgaatcgat gaccttacta gtagtaaccc catctttagg
    11881 caaagagtga atggctctag taacctgttt aagacctaac gcttccgcaa catctttcgc
    11941 cactgcccac cattcatcat taatatcgac aaatcttata atgtgtccgt tccaaatttc
    12001 agttttcatt caacgtcctc cagttcaagc cctagcaagc tcgctaacaa ctcaacttgc
    12061 tgatacaaaa tttcttgata ctcttcaata gaaagtcgtt ctttaaactc tgcttgtact
    12121 aaatcgttca agccacgcaa tactccgctt accgttggtt cttgtggtag caatttttga
    12181 tagtccattt gttcgtacaa acattcaaac gtttcttcta gcgctttgcc ttccaagttt
    12241 gctaattctg ccgctgtgtc ttctgtaaga attgttgata atactttttc gattgcttta
    12301 ttttgtgtcg attccattat tatttcctcc tattaatccc tttgccccca cggcttgcgt
    12361 ttatttttgg tattcttcca taaagcgatc acattcatct tgtgaaattc ttacgactcc
    12421 atcaactata atcaacttta aaccgttagg aacatatttc tgcatcatag tattgtatga
    12481 aacgttcata tacttagcag cttgtgcctt gttcatatat tttggaaact ggtacttttt
    12541 ttctaataat gaaagtaacg ttccttgagt ttgctctact tgttgaacaa ctcgatcgtc
    12601 aataatttta aaagctggat catcaagcgt gagtttgtca atttttaatt ccatatttat
    12661 cccccctcgt tagcatacat ttctatacaa agattttaaa aaaatgcaag aggctcttga
    12721 atacatttct atacctctta taaactcaat ataccatcat accttttcat ttgcaagaat
    12781 tttaacatac ttttttgaac ttttatgttt cacacatatt taaatcgtga tatagtagag
    12841 aaaatatatg aatggtggtg aaaatgtggc tagaaaaaaa taccccgaag cagtggagac
    12901 aggacaaaga attagactgg ctagaattga aaagggactc tcaatggaac aacttggagg
    12961 taaattatcc ccaccggcca gtaaaggtgc cgttagtaat tgggaaaatg gctataatct
    13021 tcccaataac accaggctta aacaactttc aaaggtatta gatgtatcca ctacctatct
    13081 attaaacgga tcttatacat taagagatat gcatatgatg cccgaaatta agcgaaatgt
    13141 gcttgaaaag caattgaaaa aaactattga aaaaaataac tctataattg attctaatct
    13201 ttctaatgct atcaataaca tcgaagaaga taacctgact gtctcacaga agatttattt
    13261 aacaaacgca ataaaatatc ttaaaacagg atctcgaatt gaatttcctg acgggagtac
    13321 cccaattgct tatctcgcag ctcttctaaa tacgctttta cgatatgact cattaattaa
    13381 tgattcagaa aaaaatacag atgagaaaaa agagtatcaa gaattttatg aaaatgatct
    13441 tctaaaagag ttcacagctc ttgttactga gctaaataaa cactattcaa aataataata
    13501 aactgcctcc tccaaccgcc cccacggctt gctacactat ggagggaaaa ctattatggc
    13561 aacattcaag caatatgaaa ctaaaaaagg taaattttgg ctctatgaag cctatcttgg
    13621 aatgaacaaa atgactggca aacctgataa ggttcgtaga cgaggttaca aaacaaaaaa
    13681 agaagcccag cttgccttgt cacgcttgca agtggactac gataaaaacg gtattaaaaa
    13741 agcgaacaac gaaacgttta aagaagttta tgacttgtgg ctagaaacat ataaaaccac
    13801 tgtccgagaa gccaccttca tgaaaactga aatcaagttt agaaagtgga ttcttccaaa
    13861 atatggaaat cttcgtgtga atgaagtcac ggtaaaaaaa gcccaacaaa ttgtaaatag
    13921 ctgggctgaa actactgacc aatacaaggt acttcattca acttctaagc gtatttttag
    13981 ctacgctatc aatcttggga ttattgagac taatccacta gacaacgtat taatgcctaa
    14041 aagaaccacc actaagcata aagaaaaggt caaagtttac agtaaagaac aactgatcac
    14101 cttgtttgat tacctaaaca gcaaacctga aacttatcga aacgactttg ataaaacgtt
    14161 actacgcttt ctcttttata gcggggttag gatcagtgaa gcccttgcat tgaattggtc
    14221 agacattgat tttcaagaaa atacggtcac catcaataaa acactaagcc aatccaaaaa
    14281 tggctataaa atcagtgatc ctaaaactga ttgtagtgct ggggttcttc cactagatga
    14341 aagaacaatt tctatattaa aaaaatggca gattaatcag cggaaatata tgctgacact
    14401 tggtgtaact gatccgacta tgattttttg tggtatctat aaagaactaa tcacacacca
    14461 cgctatttat gcaagattga ttactatttg tgaaaaagct atggttccat ttctcggtgt
    14521 gcatgtagca agacacactc atgcttcaat gctcttggac gctggagcta ctatggtaga
    14581 agttcaaact agattacggc atacaaaaat tagtcagacg atcgattgtt acggtcactt
    14641 ggcaaaagaa acgaaagaaa agactgttga aaaattagta caacacttaa atattgacta
    14701 gatggcatac aatatggcat acagtaaaaa ataaaaaaag gagaaaccct atcatatcaa
    14761 ggtttctcct agctttatag tctattcttc ttcttccgcc atgaatgtat tgaatacttc
    14821 ttcaatcatg tcccattctg cgtctgtttc gatttgttct aactcgcctt ctgttccatc
    14881 ttcgttttcg atgtatgcat aagcttgtaa ttctacatct tcatcttctg aaacaccagc
    14941 aggatataaa agaacatagt tacgtccaaa ttcttcttgt ccgtcaactg ttagtaggat
    15001 ttcatataat gtttcatttc cttggtcatc caccaatgtg atgtgttcgt gtccttcatg
    15061 gtcgtgggaa tggttatgtt ctgtcataag attacctctt ttcttttatt attaaaataa
    15121 ttgcttacct gagccgtcta aataattttg taagatcatc acagctgcta atttatcgat
    15181 gacttttttt cgtttggctc ttgaagtatt tgcctgctct actagcatac gttctgcctg
    15241 aacagtggtt aatcgttcat cttgataatc aactggcaag gagaatagtt ctttgatctt
    15301 ttctccgtat gacatggaag cttctgccct tggtccgatg gaattgttca tatttttagg
    15361 taatccaact acaaaccggt ctacttcata ttgtgctact aattccttca accgatcaaa
    15421 gccaaactct tcttctgctt cattgatccg aatgatctct actccttgag ctgtccagcc
    15481 aaaggggtca ctgatagcaa cacctactgt cttagaaccg acatccaagc ccattactct
    15541 cataaattca ctccatgatt tgagaggtag tccttgacaa ttacttccat gatctcatcg
    15601 cgttcgtgac ggcgaatcaa atttcgagca tcacgataac gaggaatgta ggccgggtct
    15661 ccagaaagca agtagccgac aatctgattg attggattgt agcctttttc ttccaaagcc
    15721 cgataaacaa tcgctaatgt ttcgctcact tcttttttgc gactatcgtc aaaaccaaaa
    15781 cgtacggttt catctgtaaa acccatgatt ctacacctct ttcccgcaag gtaattccag
    15841 cttaccttct acaaccttat tttactcgaa gaaagggaaa actacaaaca aatattccaa
    15901 acttccgaat aaattaagaa acactatttg tatgtttttt tccttttttc tgtagcttta
    15961 tcaaaagtac tcttttcata aagtatacgc tatatttctt tgaatgtatg tttttacaca
    16021 attttttcat aatattcata aaaaattcga atagctgcta ctaatttttt gtttttgttg
    16081 cttgtcctgt ctcataattg atcgtgtagc catcttgaac attgtagata aagacattga
    16141 attcaatttt tttgtcttca atgctttctg cttccaattg cactcccctg gctaccagtt
    16201 cattcccctc aaaatgagga gtgacacggt agcgtacatg atgatttgtt tctttgatat
    16261 atgccgcaat atcattttcg tgagcaagca tgtatggagc attcaacgaa cgtgtccctg
    16321 tcatcaagtt tttaatgttg ttattttgac cagtcagctg gaaaccaatt aaatggctgc
    16381 gattatataa ccattgcccg tcacttaatt tcttttgttt ccaacctgtc gggtcaatat
    16441 acaaaggctc tctcttttca gtagggaaca aatcttttcc aagcatagca ttggcttgtc
    16501 ctactctgtt caatttatca atatttgaat agctttccca tgttcctttg gaaaggctaa
    16561 gatcctcttt tgtaaattcc ggctgattcc cattgatttc tatttcttga tgttttccat
    16621 catattctgg cacatccgct acttgagaag cttgtggtgt ggaatcaaaa atagttgtaa
    16681 tctgctgaaa gatatctgaa tctttttgtg tggtggaaac accgaatgcc cctgcaatca
    16741 cagcagcgac gatccccacg atcgccttgg ttgttttttt gctcatttct ttactccttt
    16801 actaacagct ccctaaatct taacatatgt tcccctataa ataaatacta gaaagaaata
    16861 gaaacagcaa aaaataaaac ctgttttaag atggcttaaa aatacaaaaa agagacggat
    16921 tcctcgtctc taatcagtca ccactctatc tgtaaggtat ttattattat tcaaaaattt
    16981 tcttcatact caagacaata cactgatttc acaaccgctt ggtaaacggt gcgcaaaagc
    17041 ttgcatctgc ggtaataagt ccatccaagt taaaaacata agaagttgtt tttgcttggc
    17101 tgttcttatt acttgttgca gagcagcttt ttcacaacct attttaagcg ggtgacggga
    17161 atcgaacccg cgacatcagc ttgggaagct gggattttac cactaaacta cacccgcaat
    17221 acaaaaacag tatacgcctc ttttattcct tcctcaagtt tttttattct ttacgcttct
    17281 ggtatgtttt gtagtaaaat aaaactaatc aatcaattat tggagaagat gctatgacgg
    17341 gaaaagaatt agatctgttg ctggacactt gtttactcgc tgggaaaatc atgatggaaa
    17401 gcaatgcgga gatgtatcgg gtagaagata cgatgagtcg aattgcttta gcttctggaa
    17461 atttccgttt ggtcagctat gtcactcaaa ccggtctctt tattggtctt gatcgtacct
    17521 cgaccatccg aatggaacaa attaccaacc gttcgattaa tttagaaaaa gtagtaaaag
    17581 taaatgatct ttcacgtaaa tatgttgctt cagagctaac attagaagaa ctttactgcg
    17641 aactgcaagc aatcgaccga gaccgttctt tctttcctgt ctggttgcaa atcgttagcg
    17701 cggcagtgat cagtggaaca attatggtgt tatttggcgg aaagatgacc gatttacctg
    17761 tgactttagt cattggagga ttgggatacg cagcttatct atatagtttg aaatttttta
    17821 gaatcaaatt tttatcggaa tttttatctt ctttattgat tggcatagcc gctatctaca
    17881 gcgtacgttt tggcttagga agcaatcagg atctaatcat tatcggatgt gtgatgcctt
    17941 tagttcctgg agttcaaatt acgaacgcta tccgtgattt gctagctgga cattatgtct
    18001 caggagtttc tcgcggaaca gaagcgatga tgactgctac gatgatcggt tttgcgattg
    18061 cttttatttt ccagcttttt tattaggaaa ggaacacaag tatggggacg atatttattc
    18121 agttttcttt tagtttttta gcgactgcgg catttgccgt tattacaaat gtacctaggc
    18181 gttcattgat ttgttgtggt ctttctggtg cttttggctg gatgatctac tggatctgtg
    18241 ttcaaatagg cggatctgct gcttttggtt cattgcttgg ttctttagga gtagcattca
    18301 tcagcgatct tttctctaaa cgtttgaaaa tgcccgtaac gattttcaat attcccggga
    18361 tggttcctct agtccctggt ggtttagctt atcaagcggt tcgaaatctc gttactgaat
    18421 cctatcgaga agcggctcat tatgcggtcc aagcaattat gattgctgga gcaatcgctt
    18481 taggtcttgt attatcagaa gtctttaacc ataacatccg taatttcaaa gtaaagcgtg
    18541 acgcaatcat gatgaaacga aaaaaacggc tttttaaata aacgaagtgt aacaaagaaa
    18601 aggttgtgaa agagacgttc agccttgagc gatgacaaaa tatctcacaa acattgcgac
    18661 aagtaaccgc aggagcaata agaagtaatt atgagtatta aaaaaagttt tgtaaaaata
    18721 gcttctcaca tttttacata aaattgactt aatactgaga aactgacttt tgaacaccct
    18781 ctatctgtaa aaaaggggct gcgacatgat ttttgtcacg gcccctcttc tttattcaaa
    18841 ttttttccaa aacgttcgtt gataataaac gatccataga gcggaaagta ttagaactaa
    18901 accgatcaca ggaatattca ctaaccaaaa tggataaaac aaagccaaca ttccgtataa
    18961 gacgataaga ataatactga tgatcaatga acccatcata gttgcgacta aacccaaact
    19021 tcctgaacct ctgttaaata attggttaat attggtccag ttcagcagca gcaaccgata
    19081 atctctagcg aaataacgta gtgacaataa gtagcagccg aataaagaac caaatagcaa
    19141 gctagggata aaaaacaaag gcagctgaaa agacagtccg cttaataatg cgatcccacc
    19201 agttaaaaaa ctctgcaata tccatccgaa tctgaacttt tcttttaaat attggttcat
    19261 agagattggc aaagaacgga taaacagaaa attttcttga tccaaagaaa taagattgct
    19321 gataaaagaa gtctgattaa ctgttaagaa agccaatgcg atccctacca ggaaaacaac
    19381 accgattaac ttattatcta aatcagttaa ttttatttcg cctcctaggg caaaagcaag
    19441 aataaaaatg attggagtta acaaggaaga agaaagaact tgcataatca aattaggttc
    19501 ttttactaat tgagcattgt aactaaataa aagctgatgc acattttggt tctttttatg
    19561 ggttcttttt attttgaact cactgtttga atcagtcagt tgctctgtaa gctttggtac
    19621 tatcagctgt tttacagcaa ggaaaaatcc cagagccgtg atagctagta ttccccaacc
    19681 caataaacct tgttgagaaa gtggctgact ggcagcatca tagaatggca ataatatcga
    19741 aatcggatgc cgatctgtta actcattttc aatagtcccc gtttgatgat tcatccacat
    19801 gattccagca aatgcgatca gcatcgagct gccaagcaag atactagaaa ctgtttttct
    19861 atgtttttta aacactgctg ttcgagccaa accaaaaaca atcaaactag ccagacaaaa
    19921 aagaataatc aaaaatagag aaaacaaaag gattgagata atgatagtaa gcaaaatccc
    19981 tagatcagct cgccatccgg ttaaaataaa catgactaga agcggaaaaa caaaaggaat
    20041 gatggtcaaa gcaactacca aaatctttgc tgaaaaaacc tgttcttgtc caaaaggcag
    20101 aggcaaaaat acttgcaggt catttccttc aaaaaagata ttataaatga ctgatatact
    20161 ttgagttaaa cctaacatac caaaaagtgc cacgtaataa gtaaagaaac ctggcaattt
    20221 aacaaagttc atagcaaaca tagcaaatcc gtaaatcagt aaaaaaatca gccctgaaaa
    20281 aagattttga gacaaaagat agatagttaa tcgctttccc ttttttcctt tctctcttgc
    20341 tttattcgtg agctgtggat taacatataa aagattaact cgaattaatt ctaataattg
    20401 tcgcttatac atacggtcca tctcctgtcg cttgccttcc agccattttc aaataaatct
    20461 tttctagcgt ttcatcaggt gagccattca ataattcact aactgccccc tgataaatca
    20521 attcaccttt cttcaaaata gccagtttat cgcataactg ttgtgcggta tccaatacat
    20581 gtgtagaaaa aatgactgtt tttccttttg cggcatgagc cttcatcatt tctttcaaat
    20641 caaatgctgc ttgaggatca agtccttgca aaggttcatc caatacccaa atatctggat
    20701 ctggcagcaa cgtgccgatc aaaatcgttt tttgacgcat tccatgtgaa aaacttgcga
    20761 ttgtttcatt ttgatgacta tacatatcaa aaagcgctgt caattctgct aaacgctttt
    20821 cttttttttg tgtgtctaat tcataggcag ctgaaatcaa gtcccaatat tctccagccg
    20881 tcaattgtaa aaaaatatct ggggaatcag gcacataacc gattttttgt ttgatactca
    20941 atcgattttc cgtcaatttc attccatcta ctaaaatcgt tcccgatgat ggttcaataa
    21001 tactaactaa acttttaatc gtagttgatt ttccggcacc attatgtcct aaaaatccaa
    21061 aaatttcccc ttgtcttatc gagagagaca agtcttttaa tgcttgtttt ttcccgtaac
    21121 ttttacttac attgatgaat tcaatcattt ctgtactcct tttgtttgtt attacttttt
    21181 attgtaccct tcgttattgg tggagtaaat gtcaaaaaga gtgcataaca aaaaaggata
    21241 gaaacgacta ggctgctact tgggttgttc tcttctacag gaaaacactc ttctaacagg
    21301 taagatatga gtctttgttt tctttaaaat gattcatttt ctatcttttc ttgcataaaa
    21361 ttctttagtt tatcaatact tctctttccc atctctagtt gttctccatt tgcaaatgtt
    21421 actatttcgt ttactctgct aatagatgta atgttttcag tattaatgat ataagctttt
    21481 aaatctttac ataaatactc atattcgagt gattttttat attttttaat tgtgccgttt
    21541 acgagaatct cttcatcttt caatactagg cctattttat ttttataacc tggcatgctc
    21601 attagataaa ggatttcatc gtaatggata tagtgatcgc tattaccata acggacagtt
    21661 gtccaccttt ttttcgtagc atgttgaaca ggatagtttt ctttttcttt caataagtaa
    21721 ctttggatgc tttttttatt tttttcataa gtgaattctt ttatcaaata accactaggc
    21781 gtaatctttc gattaataac agttattgct ttattcggat gagacgttag ataaacaatt
    21841 ttacactttt cattcttttc tctaagcatt tcgcctagtt cgataccgct ataataggcg
    21901 ttcaaatcga tgtctaggaa aaaaatatct gaggaataga tttcattatt tttaatgaat
    21961 tcaacaaatt gtataacgtt caatccctga acgatctcaa atttccaagg acttaataaa
    22021 attctggatt cttccaaaat ttctactagc attttttcaa aaaccaaact atcatccaaa
    22081 ataaatatac gcgtta
//
LOCUS       DACDSY010000032        21988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDSY010000032 DACDSY010000000
VERSION     DACDSY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.032"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IWK44_001035"
     CDS             <1..395
                     /locus_tag="IWK44_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549575.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="IWK44_001036"
     CDS             398..1147
                     /locus_tag="IWK44_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9549576.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="IWK44_001037"
     CDS             1159..1428
                     /locus_tag="IWK44_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549577.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1590..1892
                     /locus_tag="IWK44_001038"
     CDS             1590..1892
                     /locus_tag="IWK44_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549578.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="IWK44_001039"
     CDS             1889..2050
                     /locus_tag="IWK44_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9549579.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="IWK44_001040"
     CDS             2047..2352
                     /locus_tag="IWK44_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549580.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="IWK44_001041"
     CDS             2352..2708
                     /locus_tag="IWK44_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP9549581.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2668..2913
                     /locus_tag="IWK44_001042"
     CDS             2668..2913
                     /locus_tag="IWK44_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549582.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="IWK44_001043"
     CDS             2910..3329
                     /locus_tag="IWK44_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP9549583.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="IWK44_001044"
     CDS             3326..3883
                     /locus_tag="IWK44_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9549584.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="IWK44_001045"
     CDS             3880..4176
                     /locus_tag="IWK44_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549585.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="IWK44_001046"
     CDS             4253..4666
                     /locus_tag="IWK44_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP9549586.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="IWK44_001047"
     CDS             5012..5386
                     /locus_tag="IWK44_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549587.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5616..5960
                     /locus_tag="IWK44_001048"
     CDS             5616..5960
                     /locus_tag="IWK44_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9549588.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="IWK44_001049"
     CDS             5965..6246
                     /locus_tag="IWK44_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549589.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="IWK44_001050"
     CDS             6349..6663
                     /locus_tag="IWK44_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP9549590.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="IWK44_001051"
     CDS             6641..8335
                     /locus_tag="IWK44_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9549591.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="IWK44_001052"
     CDS             8355..9533
                     /locus_tag="IWK44_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9549592.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="IWK44_001053"
     CDS             9496..10182
                     /locus_tag="IWK44_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP9549593.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="IWK44_001054"
     CDS             10182..11342
                     /locus_tag="IWK44_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9549594.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="IWK44_001055"
     CDS             11352..12227
                     /locus_tag="IWK44_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549595.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="IWK44_001056"
     CDS             12224..12535
                     /locus_tag="IWK44_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549596.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="IWK44_001057"
     CDS             12525..12878
                     /locus_tag="IWK44_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9549597.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="IWK44_001058"
     CDS             12868..13269
                     /locus_tag="IWK44_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549598.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="IWK44_001059"
     CDS             13262..13666
                     /locus_tag="IWK44_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549599.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="IWK44_001060"
     CDS             13678..14286
                     /locus_tag="IWK44_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9549600.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="IWK44_001061"
     CDS             14306..14668
                     /locus_tag="IWK44_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549601.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="IWK44_001062"
     CDS             14671..14853
                     /locus_tag="IWK44_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549602.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="IWK44_001063"
     CDS             14870..18289
                     /locus_tag="IWK44_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9549603.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="IWK44_001064"
     CDS             18340..19077
                     /locus_tag="IWK44_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9549604.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="IWK44_001065"
     CDS             19087..21378
                     /locus_tag="IWK44_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549605.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="IWK44_001066"
     CDS             21402..>21988
                     /locus_tag="IWK44_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549606.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3246 c         4705 g         6104 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatctg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DACDSY010000033        21435 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDSY010000033 DACDSY010000000
VERSION     DACDSY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.033"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            126..1394
                     /gene="rseP"
                     /locus_tag="IWK44_001067"
     CDS             126..1394
                     /gene="rseP"
                     /locus_tag="IWK44_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP9549607.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            1462..3171
                     /locus_tag="IWK44_001068"
     CDS             1462..3171
                     /locus_tag="IWK44_001068"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP9549608.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            3383..7741
                     /locus_tag="IWK44_001069"
     CDS             3383..7741
                     /locus_tag="IWK44_001069"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP9549609.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            7886..8359
                     /gene="rimP"
                     /locus_tag="IWK44_001070"
     CDS             7886..8359
                     /gene="rimP"
                     /locus_tag="IWK44_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP9549610.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            8382..9557
                     /gene="nusA"
                     /locus_tag="IWK44_001071"
     CDS             8382..9557
                     /gene="nusA"
                     /locus_tag="IWK44_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP9549611.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            9579..9872
                     /locus_tag="IWK44_001072"
     CDS             9579..9872
                     /locus_tag="IWK44_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP9549612.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            9869..10180
                     /locus_tag="IWK44_001073"
     CDS             9869..10180
                     /locus_tag="IWK44_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP9549613.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            10193..12499
                     /gene="infB"
                     /locus_tag="IWK44_001074"
     CDS             10193..12499
                     /gene="infB"
                     /locus_tag="IWK44_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP9549614.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            12526..12873
                     /gene="rbfA"
                     /locus_tag="IWK44_001075"
     CDS             12526..12873
                     /gene="rbfA"
                     /locus_tag="IWK44_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP9549615.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(13030..13203)
                     /locus_tag="IWK44_001076"
     CDS             complement(13030..13203)
                     /locus_tag="IWK44_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9549616.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            13651..16013
                     /locus_tag="IWK44_001077"
                     /pseudo
     CDS             13651..16013
                     /locus_tag="IWK44_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            16381..17304
                     /gene="truB"
                     /locus_tag="IWK44_001078"
     CDS             16381..17304
                     /gene="truB"
                     /locus_tag="IWK44_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP9549617.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            17308..18249
                     /gene="ribF"
                     /locus_tag="IWK44_001079"
     CDS             17308..18249
                     /gene="ribF"
                     /locus_tag="IWK44_001079"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP9549618.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            18601..19386
                     /locus_tag="IWK44_001080"
     CDS             18601..19386
                     /locus_tag="IWK44_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9549619.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            19399..20250
                     /locus_tag="IWK44_001081"
     CDS             19399..20250
                     /locus_tag="IWK44_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP9549620.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(20374..20826)
                     /locus_tag="IWK44_001082"
     CDS             complement(20374..20826)
                     /locus_tag="IWK44_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9549621.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7337 a         3757 c         4638 g         5703 t
ORIGIN      
        1 agacgcaggt ttttattttt cattatattg atactattgg tgtctttggt atactatggt
       61 aaaattatag tgttttatcg ttgttttata aaaaacacaa aaaacaagta aaggagaaaa
      121 aagctatgaa aacgattctg acatttatca tcgtttttgg tatattagtg attgttcatg
      181 agtttggtca tttcttcttt gcgaaacgat caggaatcct cgttcgcgaa tttgctatcg
      241 gtatgggtcc gaaaatttat gggcatcaag caaaagacgg gacaacatat acgcttcgcc
      301 tattacctat tggcggttat gttcgaatgg caggcaatgg ggatgatgaa acagaaatgg
      361 caccaggaat gcctctttct cttttactaa attctgacgg catcgtagag aagataaatt
      421 taagtaagaa aatccaattg acaaatgcta tcccgatgga attgagccgt tatgatttag
      481 aagatgagct gactatcact ggttatgtga atggagacga aacagaagta gtaacctacc
      541 ctgttgatca cgatgcgacg attattgaaa acgacggaac agaaatcaga attgcgccaa
      601 aagacgtaca gttccaatcg gctaaattgt ggcagcgtat gctgaccaac tttgctggac
      661 ccatgaataa ctttattttg gcaatcctgt tattcatcat tctggcattt atgcaaggtg
      721 gtgttcaagt gacgaacact aatcgcgtag gagagatcat gcctaatgga gctgcggcag
      781 aagcaggctt aaaagaaaat gatgaggtag tcagtgtgga cggaaaagaa atccattcat
      841 ggaatgatct gacaacagtc attacgaaaa atccggataa aacattagac tttaagatcg
      901 aacgagaagg tcaggtgcaa tctgtcgacg tgacaccgaa atctgtagag tcaaatggcg
      961 aaaaagttgg ccaacttggt ataaaagccc caatgaatac aggttttatg gataagatca
     1021 ttggagggac gcgtcaagct ttcagcggtt cattggaaat tttcaaagcc ttaggatcac
     1081 tatttacagg tttcagttta gataaattag gcggacctgt catgatgtac caattgtctt
     1141 cagaagcagc aaatcaaggt gtaacaactg ttattagttt gatggcgctt ctttcaatga
     1201 atctcggaat cgtcaatctg cttccgattc ctgccttaga tggcgggaaa ttagtcttga
     1261 atatttttga agggattcgc gggaaaccat taagtcaaga aaaagaagga atattgacat
     1321 tagctggctt tggattttta atgttgttga tggtgctagt tacatggaac gatattcaac
     1381 gattcttttt ctagaaaaag ttgacaaata gggtataata acaaacgata actattttga
     1441 ggaaataaag gagtatatag aatgagacaa tcaaaaatgt tgattccaac attacgcgaa
     1501 gttccaaatg atgcagaagt attaagccat caaatcttgc taagagctgg atatatccgc
     1561 caagtatcag cgggtattta ttcatatctg ccattagcaa atcgtgtatt ggaaaaactg
     1621 aaacgcatta tgcgggaaga atttgaaaaa atcggagcgg tagaaatgct gatgcctgcc
     1681 atcttgccag cagaactttg ggaagaatcc gggcgttacg aaacatatgg accaaacttg
     1741 tatcgtttca aagaccgcaa tgatcgaaaa atgatccttg gaccaactca cgaagaaact
     1801 tttactgatt tgatgcgtaa tgaaatcaat tcatataaga aattaccttt aaatctttat
     1861 caaatccaac ctaaatatcg tgacgaaaaa cgacctcgct tcggtttgct tcgcggaaga
     1921 gagtttatca tgaaagatgc gtattctttc catgcaacgg aagaaagttt ggacgagaca
     1981 tataaagatt acgaaaaagc ctacaccgaa gtattcaagc gttgtggctt agaatttcgc
     2041 tcgattatcg gagacggtgg cgcaatgggc gggaaagact caaaagaatt catggcgatc
     2101 tcagaaatcg gggaggatac gatctgttat tcgactgaaa gtgattatgc tgcaaacttg
     2161 gagatggcta caagctacta tgtatcaaaa aaatcgcatg aaacacagct tgaattggag
     2221 aaagtagcta cacctaacgt aaaatcaatt gacgaagtag cagatttctt ttctgttgaa
     2281 cctcaaaaaa tcatcaagtc tgtattgttc attgcggatg aacaacctgt ccttgttttg
     2341 gtacgcggag accatgaagt caatgacgta aaactgaaaa actttttatc tgctgacttt
     2401 ttagaagaag caacagaaga agaagcacaa aaatatctag gtgctgagtt tggttctgtc
     2461 ggaccagtcg gtgtttcaga tgacgtaaaa gtatatgcag accgttatgt ccaagattta
     2521 gcgaatgcca ttactggagc gaacgaaaca ggattccact ataaaaatgt gaatccagaa
     2581 cgcgatttca ctgtactttc ttatgaagat cttcgttttg tacaagaagg agatccttca
     2641 ccagacaaca atggtgttct tgcctttact cgaggaatcg aaatcggaca tatcttcaaa
     2701 atcggcactc gttacagtga atctatggga gcaactgtac tagacgaaaa tggacgagaa
     2761 aaatttgtga ttatgggctg ttatggtatc ggtgtaagtc gtttattatc agcaatcgta
     2821 gaacaacatt cagatgaaca aggaatcaac tggccaaaag gaattgcgcc atttgatttg
     2881 catatcgtcc aaatgaatct aaaagacgaa taccaaacaa acctgacgaa tgaactagaa
     2941 gaagcgatga caaaagctgg ctatgaagta ctggttgatg acagaaatga acgcgcagga
     3001 gtgaaattcg cggattctga tttgattggc tgtccaatca gaatcactgt cggtaaaaaa
     3061 gcagtggatg gaatcgtaga agtgaaaatc aagaaaactg gcgaaatggt tgaagtacgt
     3121 aaagaagaat tggtgaatac tttaccaatc ctcttgaacc aaggcgaata ataagcgtta
     3181 aaacacagaa aataaaaata gctgaatcag ctgtttttga caatctgatg attcgctttt
     3241 aaaattggaa ctgcgatttg aatcgcagtt ccaatttttt aatgtaaaac tcagaaaaaa
     3301 cgagtatact aaaaggtact gaaaaagaag aggataagat gcgatcaatt gcattgtttc
     3361 tcatcagaaa gggcgtatat ctttgtcaga aaaacgagat ttatttgaaa aactgttgga
     3421 acaaatccaa ctagaggaag cagaaaaaaa tcatccactg cttactgctg gtgaaatgga
     3481 tagtgtagtg gtccatcgta aatcgcgttt atgggaattt acgctgcatt tttccaaaat
     3541 tttgcctatt atgctatatc ggtcattgac tcagcatctg actatcgctt ttaaagatat
     3601 tgcacaagtc aaactgaata tcttagcaga tgaccagaca ttcgatgaac agctcttgca
     3661 agattactgg gcccaagctt tggaaaatca gcaatgcgat acaccactag cacagcaagt
     3721 attgaaaacg caggtacctg tgataaaaga tcgtaaagtc atcttgccaa tcgacagtac
     3781 aggagctatt tcttatttga agcaacaata cttgccatta gtagaagagt tgttcgtcag
     3841 ttacggattc ccaaaatttc gtatcgaacc agaagtggat gagcagcaag cggaacgagt
     3901 attgaaactt tttgaagaac gcaaacaaga acaggcagaa gcctttatga agcaagcagc
     3961 tgaaagtcta gttgtccacg aacaaaagaa aaaggaaaga aaagagcaat cgccagctct
     4021 tgaaggaccc atccacatcc agctaggacg aaatattcca gccgatgaac caacgacacc
     4081 aatgatcagt atcgttgaag aagaacgaag agtcacactt gaggggtacg tattcgacaa
     4141 agaagtcaga gaactacgtt caaaacgcaa gatcttgact ttgaaaatca cagattatac
     4201 ttcatcattt atcgtcaaaa aattctcgaa cggcgaaaaa gatgaacaag tatttgatgc
     4261 gatcagtgtc ggtagctggt taaaggtacg aggaagtatt caagaagata cgtttgtccg
     4321 tgatttggtc atgaatgcgc aagatatcat agaagtcaaa catacacctc gcaaagatta
     4381 tgctcctgaa ggggaaaaac gagtagagct tcatgttcat agcaatatga gtacgatgga
     4441 tgcaacaaac agcatctctg atcttgtggc acaagcaggg aaatggggac accgagctat
     4501 cgccattaca gaccacggtg gtgcgcaagc ctttccggaa gcacatagtg ctggaaaaaa
     4561 agcaggtgtg aaaatcttat acggcgtgga agcgaatgtc gtagatgatg gtgtcccaat
     4621 tgcttataat gatgcacatg aagcattgag tgaagccaca tatgtcgtat ttgacgtgga
     4681 aacgacagga ttgtctgcag tatatgacac gatcatcgag ctagccgcag tcaaaatgta
     4741 taaaggaaat gtgatcgaaa gctttgatga atttatcgat ccaggacatc ctttgtcacg
     4801 aacaacggta gatctgactg gtatcacgga tggaatggtt cgaggatcaa agtcggaaga
     4861 agaagtattg cggatgttcc ttgaattttc aaaagataca attttggtag cccacaacgc
     4921 ggcttttgat atggggttct tgaatactag ctacgcaagg tatggtattc ctgaagcggc
     4981 caatccagtc attgatacgt tggaattagc tagatatctg tatcctcaat tcaaacgttt
     5041 cggattaggt gtgttatcta aaaaatttgg tgtcagcttg gaacagcatc accgagcaat
     5101 ctatgatgct gaagcaactg gtcacttagc atggatcttt gtgaaagagg caatggacaa
     5161 tcataacatg ctttatcatg atcagctaaa tgagcatatc ggagaaggag attcttacaa
     5221 aagggctcga cctttccatg taacgatttt agcaaaaaat caggcaggac tgaaagattt
     5281 gttcaaactg atttcaatgt cgaatgtcga atacttcgaa cgcgtcccac gaataccacg
     5341 ctcccaattg aaaaagatgc gtgaaaatct attgatcggc tcagcttgtg ataagggaga
     5401 aatattcgaa gccatgatgc aaaaaggggt agaagaggca agaaaccgag caaaattcta
     5461 cgattatatt gaagttatgc caaaagcggt ctatgcgcct ttgatcgagc aagaattggt
     5521 aaaaaatgaa catgatttgg aagaaattat ccaaaatttg gtagaaatcg gaaaaagttt
     5581 ggataaaatc gttgttgcaa caggtaatgt acattatctg aacgaagaag atgccattta
     5641 ccgaaaaatt ttgatcaatt ctatgggcgg agcgaatccg ttgaatcgtc atagtttgcc
     5701 ggatgtccat ttccgaacaa ccgatgagat gctaactgca tttcactttt taggggaaga
     5761 aacagcgaaa gagatcgtag tagaaaatac gaataaaatc gcagatatct gcgaagaagt
     5821 gattccagtc aaagatgaac tttatacacc gaagattcca ggttcagaag atgaaatatc
     5881 agaattgagt tacacaaaag caaaacaaat gtacggagat ccgttacctg agatcattca
     5941 aaaacgatta aaaaaagagt tgaattctat taatggtaat ggcttctcgg ttatttatct
     6001 gatcgcccaa aaactagtgc ataaaagtaa tgaagatggc tatctagtag gttcacgtgg
     6061 ctctgttggc tctagctttg ttgcgacgat gacaggaatc actgaagtta atccgttagc
     6121 ccctcattat tattgtccag agtgccaata ttccgagttt tttgaagatg gtacgtatgg
     6181 ttctggattt gacatgccag aaaaacaatg tccaaaatgt ggtgcacgct taaacaaaga
     6241 cggtcatgat attccatttg agacattttt aggattccat ggagacaaag tgcccgatat
     6301 tgatttgaac ttctcaggtg attatcaagc agaagcccat aactatacga aagtattgtt
     6361 tggcgaagac tatgtttacc gtgccggaac gattggtaca gtcgccgaca aaacagcata
     6421 tggctatgta aaaggatacg aaagggacaa taatctgcag tttcgaagtg cagaagtcga
     6481 cagattagca aaaggcgcta ctggagtcaa acggacaacc ggccagcatc caggggggat
     6541 catcgttatt cccgattata tggatgtcta tgattttacg ccgatccaat atccagcaga
     6601 cgatcaaaat tcggaatgga aaacgactca ttttgatttt cactcgattc acgataatgt
     6661 attgaaatta gatattctag ggcatgatga cccaactgtt attcggatgc ttcaagattt
     6721 atcagggatc gatccacaaa cgatccctac ggatgacccg gaagtgatgc ggatatttgc
     6781 cggtcctgaa gtacttggag taagtcagga acaaatttat tccaaaacag ggacattagg
     6841 tattccagaa ttcggcactc gttttgttcg agggatgcta gaagaaacgc atcctacaac
     6901 ttttgcagaa ctactgcaaa tttccggact atctcatggt acagacgtat ggttaggaaa
     6961 tgcggaagaa ttgattcgac gaggagatgc gacactggcg gaagtaatcg gctgtcgtga
     7021 tgatattatg gtttatttga tccatgctgg attagatagc gggatggcat ttaaaatcat
     7081 ggaaactgtt cgtaaaggac aatggaacaa gatccctgat gaattgcgag aaacctatct
     7141 atcggcgatg aaagaaaata atgtgcccga ctggtatatc gattcttgtt caaaaatcaa
     7201 atatatgttt ccgaaagccc atgctgcagc ttacgtattg atggctcttc gtgtcgctta
     7261 tttcaaggtt tatttcccta ttttgtatta ctgtgcatac ttttctgtac gtgcagacga
     7321 ctttgatcta gtctccatgt gcaaaggaaa agatgctgtg aaacaagcaa tgaaagaaat
     7381 cacggacaaa ggattagatg cttccgtaaa agaaaagaat caactgacag tgctggagtt
     7441 agccaatgaa atgctggaac gaggattcaa atttggaatg attgatttgt ataaatcgga
     7501 tgccgttaac tttgttattg aaggtgatac gttgatcgca ccatttagag ctgtcccaag
     7561 tttgggaacc aacgtggcaa aacaaatcgt tgaagcaaga aaagacggtc catttttatc
     7621 caaagaagat cttgccaccc gcggtaaagt ttcaaaaaca ttgatcgaat atatgaacga
     7681 caatggcgta ttgaaagatc taccagatga aaatcaatta tcgttattcg atatgctata
     7741 agcatctgtt ttcctgtctg cttgtaattt taagcagagt atggtatagt tcttataggt
     7801 aatgatacta actgtgagtg agcggatttt tcgctcactc tttttaatgg aattacgcac
     7861 ttaatttttt aaggaggcga aacttttgag tagcgtcgtt gaaacagtca cagaaatggt
     7921 gacaccgatt ttagatgaac aaaattttga acttgtcgaa gtcgaatttg taaaagaagg
     7981 aaaaagctgg ttcttacgag tatttattga caaagaaggc ggtatcgata tcgaagaatg
     8041 cgcctttgtc agtgagaaac tgagtgaaaa acttgatgca gcagatccag atccgattcc
     8101 tcaagcttat ttcctggagg tatcctcccc gggagcagag cggccattga aaaaagaaag
     8161 cgactatgaa aaggcacttg gtgattatgt ccatgtctcc ttataccaac cagtagatgg
     8221 cgaaaaacaa tatgaaggat ttcttcaatc atttgatgct gaacagctta cattgaaaat
     8281 acgcataaaa acacgagaaa aagaattgac ttttgaccga aaaaatattg caaaagctcg
     8341 cttagctatc caattttaat cacggagagg aatcagaaaa aatgagtaaa gaaatgttaa
     8401 atgcgttaga cgctttagaa gctgaaaaag ggatctcaaa agaaatcgtg atcgatgctt
     8461 tagaagcagc attggtttct gcatacaaac gtcactatgg ccaagcacaa aacgtcgaag
     8521 tggaatttga tcagaaaaaa ggaaagatcc atgtctatgc ggtaaaagaa gtaacggaag
     8581 aagtgatgga ttcacagttg gaagtctcat tgaaagatgc acttttaatc aatccagcat
     8641 atgagatcgg tgatacgatc cgttttgaag taacaccaaa agattttggg cggatcgctg
     8701 cgcaaacggc taaacaagtt attttacagc gtgtacgtga agcagaaaga acgattattt
     8761 ataacgaatt cagtgcttat gaaaaagaca tcatgcaagg aatcgtcgaa cgccaggata
     8821 aacgttatat ttatgtcaat cttggtaaaa ttgaggcagt tctttctaaa caagatcaaa
     8881 tgccaaatga attttatcaa ccacatgatc gaatcaaagt ttatgtttcc cgagtagaaa
     8941 atacgtctaa aggtccgcaa gtatttgtta gccgcagcca tccagaccta ttgcgccgct
     9001 tatttgagca agaagtacca gaagtctatg atggaatcgt tgaaattgtc agcatcgccc
     9061 gtgaagctgg cgatcgttca aaagtggcag ttcgctcaaa tgatccaaat atcgatgcag
     9121 tcggtacatg tgtcgggcca aagggacaac gagttcaagc aatcgtcaat gaattaaaag
     9181 gcgaaaacat ggatatcgtc gaatggaatg aagatccagc tgtattcatt gcaaatgcat
     9241 tgaatcctgc acaagtcatg gatgtgatct tcgatgagac aaatccaaag gcctgcacag
     9301 ttgtcgttcc agactaccaa ttatcgctag caatcggtaa acgcggacaa aacgcacgtt
     9361 tagctgctaa attgactaac cataaaatcg atatcaaatc agaatctgac atgacggaat
     9421 tttatgaaaa tcaagaacaa caaaaagaaa cagaagaact gcatgatgaa gcaatcgttc
     9481 agtcagattt aacagacgat gaatatgaaa caattgcatt caacaatgaa acggtcgaag
     9541 aaaaaccaga agcttaactt tagtaaggag gcaaagagat gaaacaaagg aaaattcctt
     9601 tacgcaaatc cgtggtttca ggtgaaatga aacctaaaaa agagatggtc cgaatcaccc
     9661 gttcaaaaga aggggaagta tcgatcgatc caactggaaa aatgcctgga cgaggagcgt
     9721 atgtctcact tgagccggaa gaagtacaac aagcttggga taaaaaaatt ctcgatcgag
     9781 tactcgaaac aacattaaca gatgagtttt atcaagaact tcttgactat gtcactcacc
     9841 aaaaagcacg taaagagtta tttggaaaat gaatacagtt aatcggcaaa aagccatgaa
     9901 tctcatcggt ctagcgatgc gcgcgggaaa actaatcacg ggtgaagaac tgaccattgc
     9961 tgaaatccgc aaacaaaaag caaaattggt gtttgtggca accgatgcaa gcgagaatac
    10021 aagaaaaaag ataaaagata agagttcata ttatgaagtt ccttgctttg agctattttc
    10081 tgaagcagaa atcacgcaga tgatcggaaa acctcgcaag gtattcggaa taatggatgc
    10141 cggatttgcg aagaagacca aggagctaat tgaaggttag gaaggtgatt gcatgggcaa
    10201 caaaagaatc tatgaattag caaaagaact caaaaaatct agtaaagacg tcgtggaaaa
    10261 agcacaacaa cttggcatcg atgtgaaaaa ccatatgggt gcgattacta gcaatgatga
    10321 gaagaaattg caacaagcgt tcaaaaaccc aagcacgaaa caacaagctg cacaacaagc
    10381 aagcagaaaa ccagccaatc aacagccgaa cgatcaacgt aaaaaagaaa caacgaacaa
    10441 tcaacaaaaa aataaaaata accgcagtta tcaagatcgc ggtcaaggga gcggtcaagt
    10501 gaatcaagga aaaaaaccaa caacaaacca aaataatacg ggaaatacca gcagccaaaa
    10561 tcgtcaagga acgagccaag gcggcaatca aaaccgtact aacaataaca acaaaccaaa
    10621 caataacaat cgcggcaaat ttaataataa taaccgtaat cgttataata aaaaaggtaa
    10681 aaaaggcaaa cagcaacaat caactaaacc agctgtacca ccacgcaaat tccgtgaatt
    10741 gccagatgta ttagagtata ctgaaggtat gaacgtagca gatattgcga aaaaaattca
    10801 tcgtgagcca gcagaaatca ttaaaaaatt atttatgctt ggtgtcatgg tcaatcaaaa
    10861 ccaagcactg gataaagata cgattgaact gttagcgacg gactatggca tggaaccaca
    10921 agaaaaagtc caagttgaca ttgctgatat cgataaattc ttcgaggcag aagaagtcga
    10981 tccagataaa ttagtctcac gtccgccagt cgtaacgatt atgggacacg tagaccatgg
    11041 gaaaacaact ttattggata cattacgcca ttcacgtgta acaagcggcg aagccggcgg
    11101 aatcacacag catatcggtg cctaccagat cgatattgat ggtaaaccaa tcactttctt
    11161 ggatacacca ggacatgcgg cctttacaag tatgcgtgca cgtggagcaa gtatcacaga
    11221 tatcacgatc ttagttgttg ctgcagatga tggcgtcatg ccacaaacag ttgaagcaat
    11281 caaccatgcc aaagctgcag gcgttccaat catcgtcgct gtaaataaaa ttgataaacc
    11341 aggtgcaaac cctcaacatg ttatgcaaga attaagtgag tatgagttaa ttcctgaagc
    11401 atggggtgga gaaacgatct ttgttgagat ctcagcgaaa ttcggccaaa acatcgaaga
    11461 attgctggaa atgatcctgt tagtcgcaga agtagaagat ttaaaagcag atccaacaca
    11521 acgcgcaatc ggaacagtga tcgaagcacg tcttgataaa ggtaaaggtc ctgtgtcaac
    11581 cttacttgtc caagaaggaa cacttcatgt tggtgaccca atcgttgtcg gtaatactta
    11641 cggacgtgtc cgtgtaatga caaacgatct tggccgtcgc gataaagaag caggacctgc
    11701 aacgccagta gaaatcactg gcttgaatga tgtacctcaa gcaggtgacc gtttcgtagt
    11761 cttcgaagac gaaaagacag cacgtgcagc gggtgaagaa cgcgcgaaac gtgcattatt
    11821 agaaaaccgt gctgccacaa gccgtgtgac tttggataac ttatttgaaa gcctgaaaga
    11881 aggcgaattg aaagaagtta acgtaatcat caaagcagat gtacaaggtt cagcagaagc
    11941 actggctgca tcattgaaga agattgatgt ggaaggtgtc cgtgtaaaaa tcgtccattc
    12001 agctgtcgga gcaatcaatg aaagtgatgt aaccttagca gcagccagca acgctattat
    12061 catcggtttc aacgttcgtc ctacaccgca ggcaaaagtg caagctgata ctgaagaagt
    12121 cgatattcgt cttcaccgta tcatctataa agccatcgaa gaaatcgaaa ctgcgatgaa
    12181 agggatgcta gaccctgaat ttgaagaaaa aatcaccggt caaatgatcg ttcgtgaaac
    12241 cttcaaagtt tctaaagttg gtacgattgc aggtgcctat gtaacagaag gatatattcg
    12301 tagagatagc ggtgtccgtg taatccgtga cggaatcgta atctatgaag gccaacttgc
    12361 aagtctgaaa cgattcaaag atgacgtgaa agaagtcaaa atgggttatg aatgtggtgc
    12421 aatgattgaa aaattcaatg acatcaaagt agatgacgta attgaaggat ttatcatgga
    12481 agaagtcaaa aatgactaaa aactgataaa aggaggccaa tcattatggc taactaccgt
    12541 gaccgccgag tcggtcaaga aatcttaaaa gaagtcaatg atattttgcg caaaaaggtc
    12601 cgtgaccctc gtgtggagaa cgtaacgatc acagatgtga aagttacggg ggacttacag
    12661 caagcaacga tctattacag cattttatct gacttagcat cagataagaa aacagcacaa
    12721 gatggattga ataaagcatc aggattgatt cgtcgagagt tagggcacaa tctaagtatt
    12781 tataagacac ctgaattgtt ctttgaactt gatgaatcag ttgtctatgg caaccatatc
    12841 gatgaattgc ttcgcgactt gaacaaagac taatcaaaaa ctcccgcaaa tcctgtgaaa
    12901 tcaggggttg cgggattttt ttgttttcat taagacgtat tacctaagag ttccatgact
    12961 ttagtcttgc ggcgtgtcag ctaaccaaaa aagagaaaaa gaaaagtaaa aaattcgact
    13021 ttcttttttc tagtagtata taaatttttg catatagata aagatcaaga taccgataac
    13081 ggaagctaga ttgtaatcta ttttagtgac agtcaggtat agccaagtaa ctagaagatc
    13141 agtggaacct gcaccataaa agtttgagtt tgtcggtccg ttgacagtta aaagaaaaat
    13201 cacgttgaag ttattgatat ttccgattac gccgcttcga tttggtcacg atccaaattt
    13261 tggataatcc ctgtagaaat tagcatagta gctgggatac caattcacat attgacaaag
    13321 ataacagaga cttttacata aaacggatca atcaggaaag gaattgctcg atccagcccc
    13381 agttttccag caatgtgtta actggcccag cgtcattcag caaattatgc atgatgagga
    13441 gcgaaatgaa ctgcggaaca gccattgtga taacgacaag ttttttggat caatgtaaaa
    13501 aacgtcagcg aaaggaaaaa tttcgtggtt ttttatacct gttcatttgg ctagatataa
    13561 aatccaaaca aatgaacaga agtgcttatt aggtttcaat tgggagtatc tgcgctagaa
    13621 taagagtaat aatgaaaagg agtgttatgt atggattatt ctagtaaaga atactttgat
    13681 aaaatgacgg cttggtggcg tgctgctaat tatctttctg ttgggcaact ttatcttaaa
    13741 gataacccct tactgagacg tacactgaaa cccgaagatg tcaaaaaaca tccaatcggt
    13801 cattggggaa cgattcctgg ccaaaatttt atttatgtcc atttgaaccg agtcatcaat
    13861 aaatatgatt tgaacatgtt ttatattgaa gggccaggac acggcggaca agtcatggta
    13921 tcgaatgctt atttagatgg aagttacaca gagatctatc cagaagtaac ggaagatgag
    13981 acaggaatgc aaaaattatt caaacgattt tcatttcctg gaggcattgc ttctcatgca
    14041 gcaccagaaa caccaggttc tatccatgaa ggcggagagc ttggatactc tctttcacat
    14101 gcagtcggag ctgtattaga caatcctgaa gtaatctctg ctgtcgtgat tggtgatggt
    14161 gaagcagaaa ccggtccttt agctggaagc tggttctcaa atgtctttat caatccagtg
    14221 atagatggag ctgttttacc gatccttcat ttaaatggtg caaaaatagc caacccaacg
    14281 atcttggcac gtaaatcaga tggagaatta gcaaattact ttaacggact tggctgggaa
    14341 cctttcttta ttgaaggaaa tgattcagaa aaattaaatc ctgttatggc tgaaaaaatg
    14401 gatcaagcaa tcgagaaaat taaatccatc caaaaagaag ctcgcctgaa gacagcaaca
    14461 gatgtcgtga tgccaaaatg gccagtattg atcgtacgca caccaaaagg atggaccggt
    14521 ccaaaagaat gggacgggga accaattgaa ggaaccttcc gagcacacca agtaccgatt
    14581 cctgtagatc aagaacatat ggatcatgca gatgctttac tccgttggct gaaatcctat
    14641 gaaccagaaa aattatttga tgcacaagga cgtattttag aagaaatcag agaaatcgcg
    14701 cctactggtg accaaagaat ggcaaaaaat cctatcacca atggtggaat cgatccaaga
    14761 ccgcttatta tgccagattg gaaaaaatat actttgcaat ttgaaaaacc aggctccgtt
    14821 aaagcagaag atatgaccga acttggtaaa ttcgttcgcg agatcattga gaaaaatcca
    14881 gaaaatttcc gtatatttgg accagatgaa acaaaatcaa accgattgaa tcaagttttc
    14941 aaaacgacaa atcgccaatg gatggaaaaa atcgaaccag aaaatgatga atggctttcg
    15001 ccatctggtc gcgtcatcga cagtcaatta tcggaacacc aagatgaagg gttcttagaa
    15061 gggtacgttc tgactggacg gcatggtttt ttgcaagtta cgaatcattc ttgcgagtgg
    15121 ttgactctat gttgactcag cacttcaaat ggatgcggaa atcacatgac ctttcttggc
    15181 gaaatgatta tccttcgcta aatctcattg caagctcaac agtcttccag caagatcaca
    15241 atggatatag ccaccaagat ccaggaatat tgacacattt ggcagaaaag aaggcggagt
    15301 tcatccgtga atatctgcct gcggatgcaa atacattgct cgcagtcatg gataaagcat
    15361 tccgctcgtc agaaaaaatc aatttgatca tctcttccaa acatccgcgt gctcaatttt
    15421 actcagcaga agaagcagcc gtcttagtga atgaaggctt gaaaatcatt gattgggctt
    15481 cgacagcaaa agaggaggaa cctgaattgg tcattgctgc tgcaggaacg gaatctaact
    15541 tagaagcatt agcagctgta actttgctgc tggaagaatt cccaaaacta aaaattcggt
    15601 tcatcaatgt cgtagattta ttgaaattac gtcatccaag tcaagatccg cgtgggttga
    15661 gcgatgaaga atttgatcaa tattttacaa aagacaaacc gatcttattt gctttccacg
    15721 gttatgaaac attggtccgc acgattttct ttgatcgtca taaccatcat ttaatgattc
    15781 atggctacaa agaaaatgga gatatcacga caccatttga catgcgtgta gtaaatgaac
    15841 ttgatcgtta tcatttggca aaagacgcag ctttaaaaat caaaggaagt caagcagaag
    15901 attttgcgaa aaaaatggat cagaaattac aagaacatca aaactatatt cgtgaaaatg
    15961 gtattgatct gccagaagta ttggattgga aatggaaaaa tcttgaccaa taaaatgata
    16021 aaatcgatcc cgcaaaatag aattttttac attgattaag aaactgaaaa aataaataaa
    16081 aaaaacggaa aaaagactgg aagaatggcc agtctttttt ttcgtttttg ggaagtgaaa
    16141 accaaactat ttacctgtaa gccacccata aatccaatca aatggtgcaa agaaaaaatc
    16201 ttccattcta ttccctcctt taaaaaagca taacatgatt tctatttacg tgagcatgaa
    16261 ttttgttacg tttttattgc cgaaaaataa aaaccgttca ttatgatgtc aaattcgtca
    16321 gtaattgatg caatgtgtta taataattca gttaaatcaa aaatgaaatg gagaaaagac
    16381 atggacgggt tattaccttt atggaaagaa cgcggaatga caagtcatga ttgcgtattc
    16441 aaattaagaa aaatcttaca tacgaaaaaa atcggacatg ggggtacgct tgacccagac
    16501 gtagacggtg tgttgcccat ttgtattgga aaagccacaa aagttatcga atatttgaca
    16561 gattctggga aaatctatgt aggcgagata actctaggtt tcagtacaac gacggaagat
    16621 cgatccggag aacgggtaga aacaaaacca gtagatatgt ctttatctga aaaaaaaata
    16681 gatgaagtga tggcttcatt tattggtgag atcacccaga taccaccaat gtactcagcg
    16741 gtaaaagtca acggtaagcg gttatatgaa tatgcccgca ataatgaaac agtggagcgt
    16801 cctgttagaa aggctcagat ttaccgtttt atccggacaa gtgaattgac aagagacgaa
    16861 gtgacaggaa ccatttcttg gcgcttcgaa gtagaatgcg ggaaaggaac ctatgtccgc
    16921 acattagctg tagatacagg caagaaactt ggttaccctg cgcacatgtc tgatctgacc
    16981 cgaacagcaa gtgccggtat gtcccagaag gatgctctga cattagaaga agtagctcaa
    17041 gctgtgaaaa atgggacaat cgatgattgt ttgctgccaa tagaaaaagg agtggaacgg
    17101 ttcaaacgag tagatattac tgatgaagta tggcagaaag taaaaaacgg tatgagactt
    17161 gattatactg tatttgcgct cccagaaatg ccgtcagaag aaattgcttt gttttataaa
    17221 ggaaaagttg tcagtattta tcagccaaac ccgaaagaaa aaaacaaatt aaaacctagt
    17281 aaagtattga gaaatgagga ataacgaatg aatatcatca agatcagaca tccttaccaa
    17341 tcagaacaaa ttccagaaga cgatgtcgtt ttagtgctgg gatttttcga cggcgtgcat
    17401 atcggacacc agaaagtcat aaagacaggt atggaaattg cacaaaaaga aggattgaaa
    17461 ttagcattga tgactttcaa tcagcatcca tctattgtct ttaaaaagat cgatcctagc
    17521 agcgtgaaat atttgactag ccttaagcaa aaagaagaaa aaatggccgc tttgggtata
    17581 gattatttat atgaagttga ttttacttct tcttttgctc atttagcacc gcaggaattt
    17641 gttgatcaat acatcgtagg gttacatgca aaatatgctg tttccggttt tgattatacg
    17701 tatggaccaa aagacattgc agatgtcgct cattttcctg aatatgccaa aggaagattc
    17761 aaaatcgtca cagtttctaa agaagaagaa caaggtgaaa aaatcagctc tacccgtatt
    17821 cgtggattac tagacagcgg acaagtaaaa gaagcagcaa aactattagg ttgcacatat
    17881 gaggtagaag gggtagttgt ccatggcgat gcacgaggca gactactagg attccctaca
    17941 gcaaatgtca aagtaaagag cacggttcat ttgcctaaag aaggtgttta tgtgtcggaa
    18001 ataaaaattg gggaaacatg gtatccagcc atgggttcaa ttggacataa tgatacattc
    18061 ggtgaaggac gggagctgac tgtagagttg tatattttgg actttaatca agatatctat
    18121 ggtgaacatg tcgcggtacg ctggaatcaa tttttaagag atcaagtgaa attttctagt
    18181 gtagaggaat tgattgaaca actcaaggag gatgaacaag caacagctga atatttttct
    18241 cagcaataaa acattatgca taaaatctct tgtttatgta taactagcaa ttacaataaa
    18301 atgggtgata aataagccca ggaaaggggc tgtgacaaaa gtcttgtcac agcctttttt
    18361 tccaaataaa tggtttttca gaagtagctt cttcggaaat aagccgaaat ttcttgaagc
    18421 tgaccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag caattcatga
    18481 aaacgattgc tagagcgtat catttgcttt agactatata tcttggtgta cactcacttt
    18541 aaggcggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg agtgggtact
    18601 atgtacaagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa ggggattgga
    18661 acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa ctataactcg
    18721 gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg aaaagcagtc
    18781 tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga tacggcttta
    18841 gaaaattttg ggacactgga cgtttgggtt aataatgctg ggatggaaaa tcagcatcca
    18901 acccatgaac ttagccttga agattgggaa agagtgttaa aagtcaattt gactggcgtt
    18961 tttatcggaa cgaaagcggc tttaaattac tttacagaga ataataaaaa aggaaatatc
    19021 atcaatatgt cttctgttca tgaacagatc ccatggccaa cgtttgctca ttacgctgct
    19081 tcaaaaggcg gggtaaaatt atttaccgaa acggtagcaa tggagtatgc accgaaggga
    19141 atcagaatca atagtatagg ccctggtgct atccgtacac ctattaatgc ggcaaaattt
    19201 gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg cgtaggtgaa
    19261 ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag ttatgttaca
    19321 ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca aggtggaaaa
    19381 ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt tggctggggg
    19441 atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat attaggaact
    19501 ggtattggag cggtgcttgt cggcttactc gttcaattat tcgcttctcc agctggtatt
    19561 tcagtgacta catttttgat tagtttactt tctggtgcat tttgggttat tggacaaaca
    19621 ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat ttcgactgca
    19681 ttacagttag tcggaacctc gttgattggt gtcttcgctt ttggagaatg gtctggaagt
    19741 atggggaaaa tcattggagc agcagcaatc attcttcttg tatttggttc tgctttaacg
    19801 gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaattag tctattagca
    19861 tcaacaagta ttggttattg ggtatacagt gcattgccta aactagttga tgcaagcgga
    19921 ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat ttatgtgatt
    19981 ttccgtaaac caaccgcatt ttctgaagac aagagttgga aagcaacgat cgttggagcc
    20041 atctttagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg ggttgctact
    20101 gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat gtacatcctg
    20161 catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt gctgatcgta
    20221 gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaattt catttattaa
    20281 aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct agaatttcca
    20341 acatctctgg aatttctagc gctttttaat tgtttaagat acaagggaaa tatctgtctg
    20401 gatcaataat ttttcattga tccattgact aaagtttacg acataattga agttaccacc
    20461 gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac ggtctttttt
    20521 atttgcgtaa acatcgttat ggtactggac taattgttct cggaattgat tgattgctgt
    20581 aaaatcacga tattgaaggt tgaaacgcat cgtgattggt gcatttagca tgaatcgctg
    20641 catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa gattacgttc
    20701 agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat gaagataatt
    20761 tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg agtaaaaatc
    20821 tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat ttgcttaatc
    20881 acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt cagaaaattc
    20941 taacttttat ttctctaaag aaattcgttc tctcctaaag tgatattata tttaaatata
    21001 cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat tttttcctta
    21061 ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actatgtaga aaattcaaag
    21121 ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat agcagatact
    21181 ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca aaacctgtat
    21241 aaggtgatga atagtttcct cgccagtaaa aggtttattc gtataaagct ttatcgccaa
    21301 aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc aatcaattcc
    21361 cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac gtagcctgct
    21421 tcttggatat ttttt
//
LOCUS       DACDSY010000034        20903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDSY010000034 DACDSY010000000
VERSION     DACDSY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.034"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..2551)
                     /locus_tag="IWK44_001083"
     CDS             complement(<1..2551)
                     /locus_tag="IWK44_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9549622.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQE"
     gene            complement(2563..3612)
                     /locus_tag="IWK44_001084"
     CDS             complement(2563..3612)
                     /locus_tag="IWK44_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP9549623.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(3638..3952)
                     /locus_tag="IWK44_001085"
     CDS             complement(3638..3952)
                     /locus_tag="IWK44_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP9549624.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(3968..4414)
                     /locus_tag="IWK44_001086"
     CDS             complement(3968..4414)
                     /locus_tag="IWK44_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9549625.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(4415..6391)
                     /locus_tag="IWK44_001087"
     CDS             complement(4415..6391)
                     /locus_tag="IWK44_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP9549626.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(6549..7445)
                     /locus_tag="IWK44_001088"
     CDS             complement(6549..7445)
                     /locus_tag="IWK44_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9549627.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(7438..8118)
                     /locus_tag="IWK44_001089"
     CDS             complement(7438..8118)
                     /locus_tag="IWK44_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9549628.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(8119..8901)
                     /locus_tag="IWK44_001090"
     CDS             complement(8119..8901)
                     /locus_tag="IWK44_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9549629.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(8894..9811)
                     /locus_tag="IWK44_001091"
     CDS             complement(8894..9811)
                     /locus_tag="IWK44_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9549630.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(9975..10298)
                     /locus_tag="IWK44_001092"
     CDS             complement(9975..10298)
                     /locus_tag="IWK44_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9549631.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            11086..14313
                     /gene="fss3"
                     /locus_tag="IWK44_001093"
     CDS             11086..14313
                     /gene="fss3"
                     /locus_tag="IWK44_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP9549632.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            14426..14740
                     /locus_tag="IWK44_001094"
     CDS             14426..14740
                     /locus_tag="IWK44_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9549633.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            14753..15127
                     /locus_tag="IWK44_001095"
     CDS             14753..15127
                     /locus_tag="IWK44_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9549634.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            15137..15472
                     /locus_tag="IWK44_001096"
     CDS             15137..15472
                     /locus_tag="IWK44_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9549635.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            15555..16895
                     /locus_tag="IWK44_001097"
     CDS             15555..16895
                     /locus_tag="IWK44_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9549636.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            16973..17647
                     /locus_tag="IWK44_001098"
     CDS             16973..17647
                     /locus_tag="IWK44_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9549637.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            17880..18314
                     /gene="dcm"
                     /locus_tag="IWK44_001099"
     CDS             17880..18314
                     /gene="dcm"
                     /locus_tag="IWK44_001099"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9549638.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            18315..19022
                     /locus_tag="IWK44_001100"
     CDS             18315..19022
                     /locus_tag="IWK44_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9549639.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            19012..19302
                     /locus_tag="IWK44_001101"
     CDS             19012..19302
                     /locus_tag="IWK44_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549640.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            19561..20745
                     /locus_tag="IWK44_001102"
     CDS             19561..20745
                     /locus_tag="IWK44_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9549641.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            20760..20879
                     /locus_tag="IWK44_001103"
     CDS             20760..20879
                     /locus_tag="IWK44_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9549642.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         6539 a         3913 c         3947 g         6504 t
ORIGIN      
        1 attcttgttt attctccagt gcattgacaa ctgtcccaag ggaaaggata gcctcaggta
       61 ctattttttc ttgttctgtt ggattagcta agcgaagaac aaatccttgt tcatgaaaat
      121 aagaaggata aatcgatgat acaattaagt cttcaggtaa ttcaatcaat gaaaatttac
      181 gaggaacatc aagttcattt tgtaatggcc aaatcttgtt atctaacctg tgaataaata
      241 cattcagttt ctgcttttga taagaaatag attgttctaa ccgtttttct gcctgttttg
      301 ccactttgaa ctcattaaat cttccttctt ctacattaaa tccaaaagtg actttatact
      361 cacctatctc ttgagctagc ggtgtttcca tcatgacatg cccttcattt gtcgtatctc
      421 ctgaagctcg cccaggtcgc caagcaagat taggttttcc aagttcccct gttgttgaaa
      481 ataaggttat gattaattga ttttctatac gttcatactc ttttaaacca tctgcataaa
      541 acgtaaaata atgatcattt tctgccacag agacactctt atcgaatatt tctaaattaa
      601 caggtttctc taccatagtt tctgaccaat tcggaggaat gacatcaggg gtattttcaa
      661 taaatccgtt ttgaatttgt gcaattgatt tttcagattt atctaaaaca ttaattgcta
      721 agcgcatacg atgcgagtaa acagtattat gaattttgag tgtgccttca acgatttgac
      781 tttctcccag actgaacgat atttcataag atatttcttc catttcgggt ttttcagata
      841 aacgatcttt taaatcttta ggaagttgag cttttccgta aacaactaat gtttcttgta
      901 aagaatcttt atatacttta gctgtttcta attttagaga tagttctgta tctccttcta
      961 atggtgaata atcatacgta tcgccatcat ttgctgaatc gatcaaatga acgaaatcaa
     1021 gatattgtct atctccaact atcaaattca attgcccatc tttaaaagaa agtttatatt
     1081 tattgttact tatctcagtt ttgtctatct cgattgctgt ttctaatttt tcattatttt
     1141 tctcaaattc aacaacagtg tatcctagtg ctggcacatt tacatttatt tttatgtcaa
     1201 gttcataata agctggttca tcaaaatatt cgaaacctgt agcaatcatc cgataagcat
     1261 ttttccgttc tggaaaataa acttcttttt caatgatatt atagggacta cctttgaagt
     1321 gtattttctt tgaatcagct actacatgca cgattttcga tccactataa ttctcagtcg
     1381 ttgtgttgaa aacaaggact tcattcgatt ttagctttaa tgcatcagcc atccgtttta
     1441 caataaggtt ttcgattccg tcagctatct cattagcttc tttgattcga tggaaaatat
     1501 cttccgcaac attatctgaa acacagccac caatactatc atgagcctgg ttttgcaaaa
     1561 tctttttcca taaccgacga agtaggcctt tactgatatg gataccattt ctttctgcaa
     1621 tcagcatgag cggctcaact cgttttagaa ttttttgttc caatttaaaa ttttctattt
     1681 tcatttttgt tcttactgag ccaatagaac gatgaacacg agagtaaacc ggctgacgaa
     1741 gttcaccttt atattcaggt aattcttttt tgtttctgat aacttctaca taagaaggat
     1801 aatctgaaat aatattgtgg aatttactgt atttattcaa cttctctacg acttgatcaa
     1861 aatttaaaat cattgacttt tgatctacac ccgaaggtac taaaatatct ttgttttttc
     1921 cttgcatatg aagcatttca actgcttcat ccaacttttc ttttgcaaat tcaggaatat
     1981 ctttaattgc gtctaaggtc atcattccag tcatataacc aaatggaaaa ttcatggcat
     2041 aaacaaattt atctcccaat cctttccaga taaaataagg agatggaacc atcgtttcaa
     2101 agtttaatcc tcgccaacac atgaaattat cgatttctac ttgatgcaaa agtgtaggca
     2161 aattcgcatt gaacccaaac gtgtcaggca aataccctac catcattgga tctccatatt
     2221 taaggatcgt atcactgatt ccaatcatta agtttcttaa aattgattca gcatccacta
     2281 ataaggcatc tgtttgtgca taccacggtc ccacgaatag ttgtcctgtt ttcatcagtc
     2341 gttggataac cttaagcttt tgcggattga tctccaaata ctcatcgacg attgaagttt
     2401 gtccatctaa acaaaagttt actttagggt tcctttccaa ttcatctaga acttcagtaa
     2461 agacctgatc agacaatacg atggcatctt catttgtaaa ataccattct ctgtcccagt
     2521 gcgtgtgttg aattatatgt gcatttacca tatcagattc tcctactttc cttcttctac
     2581 tgaatttact tgttgttctt ttttatactt gatggatttt agtgcattga ccattaacgc
     2641 tgtaaccact cccccaataa caattgatac taaatataaa ggtatattcg taacaaaagg
     2701 aattgccata aaggttgaaa gcattgtttc ttgagtaaca ccaaaaaatc catttagacc
     2761 acatgctact gcagtgccca ccacgatgct tggtattact cgtaaaggat ctttcacggc
     2821 aaacgggatc gtcccttcag taatccccac aaggcccata atcatagctg tctttgcatt
     2881 atctcgttcc gattcatcat aaagtttttt gtttaaaatt gttgctaaaa acattccaaa
     2941 tggcggaata gcaattccta ataacatggc tgtactaggt ccaaagcttc cgcttgaaaa
     3001 agaagcaacc ccaaaagcat atgcgatttt attgataggt cctcccatat caaacgccat
     3061 cattcctcct aaaatagccg acatcaaagc agcatttgct cctgacatgc cttctagcca
     3121 attagtcagt cctgttgtca ttgcttgaat aggcgtagct agtacatacc ataatatgat
     3181 tccaacaact aatgtagtaa caaaaggaat cagcataatt gattttagac taataacttg
     3241 catcggtact tttacttttt tcaatgcatt agctaaatag ccacaagcta atccagcaac
     3301 cattcctcca ataaatccca taccaagatt ttgacacatc atcccagtga taaaagctgg
     3361 tgctagaccg ggacggtcag caattgaata tgcgatatag gctgaaatca tcggtacgat
     3421 aaactgtaat cccgtctgac caatcattcg caggactccc cagaatgtat ccgtctggtc
     3481 ccaaacacct gtgccaccaa aaatgactgc aatagaagtt agtaaccctg ctgctacgac
     3541 aagcgggatc atataggaaa ccccactttg gaaatgattt agaatttctc ttccttgttt
     3601 cttgaattcc attagtaact cccccacgat atatttttta tttgttaatt aatgcaattg
     3661 cttcatcaat cgtctcttca ccatctttta ctacctctga ggtccctact gttaaaatcg
     3721 gtttaccgtc aaagcgacct cgttctgaaa catttgtatc tactgcaaat atgacaacat
     3781 ctgcctcgga aacatccgaa attgaaagtt tgttttctat ccccatagcc ccctgtgttt
     3841 ctactttaat atctaaccct ttcttcttcg caaacttttc taaattagct tttgccatat
     3901 aagtatgggc aatccctgcg atacatgctg ttactgctac tatttttgtc atatgaaatt
     3961 cctcctttta aaatgataga gcttttacca attctttttt atttctcgaa tcttttattc
     4021 ttttaacaaa ctcgtcatcc atcaatcttc cagataattc tgctaaaata cgtaaatgat
     4081 tagtaccttt atcagtgtct tttattgcta acaaaaagac gagatcaact ggttgatcat
     4141 ctaatgcgcc ccattcaaca gaccgcttta acttggctac agccactgtt gattcaatta
     4201 ctgctaggct tttgccatga ggaatggcga tatttttccc aattcctgtg gtcatatggt
     4261 tttctctgtc aaatacgtct ttttgaaatt gtttgtggtc agacacaaac ccattagtct
     4321 ctaacaattt cgataatttt tcgactacac tttttttatc agatgcattt aaatctatca
     4381 ctatattatt tatattgatt ttttcactga tcatttataa gccttctttc aatatataaa
     4441 ctaatttttc aaacgacgaa ctcgttttta aatttgctaa taactcatga ttcgaagcga
     4501 tttcattgaa tgtttcataa attttattca attcaggttt tactttttca ttaagtgcaa
     4561 aaaagaaaac agaatgaaca agtgtcccat cccaatcaat tccttctgga ttaacgtaga
     4621 cacagattac tggctttttt ataaaatcta cttcgatatg cggcatagca aaatcattca
     4681 tcgctgttga agatagtttt tctcttttga ttgctccctc tagtatccct tctttcgcgt
     4741 aattttcttt taccaatttt ttggtaatta tatttaaaac gttcacttga ttatccttta
     4801 tttggtgggt aaaaaagcaa aatcgtttat ctaataaatt tagaaaagca tcttctgttt
     4861 gttcatttaa ctgtagatac tgttttactt ttctgacatc tgcccctaac atcaaaggac
     4921 taacaacaat aacaggaaaa tttgtatttt caatcggaat cgtgcttata acaagttcat
     4981 cagtgacttt tctatcttgt agttcattga tgcttagtac atcttctatc tcaaccatat
     5041 ctgcaaaacc gttatttatt ctttgctcta ataattttga tgttccatat ccgctactac
     5101 aaactaatat cactttcgtt ttgtcaggtt tgtaacgatc caaaagtgat tgaatatgga
     5161 gtgttacgta agcgatttca tcttcattaa aatgtataca gaatctctct tcgatttcct
     5221 ctaataaatc tacacttatc ataaatgacc ttttaaaaga atatttgatt ttgtccgtat
     5281 aagggttata gatatttact cccaatttga gtcttttaac tgctgcattg agatgtaata
     5341 ctagattatt gatcaattct tcatcaggat aaaattcatt taatttaatt cttactaact
     5401 cttgtagctc aacacccata tctaaatagg aaagcttcgt ttgatctaag tcgtgattca
     5461 tagcattttt ttcaatcgaa gtaatgtgaa tgtcaacata agcttgttca tctattggca
     5521 caataattga aaatgctttt tcaatcattt tgattaaaat aagtgtattc tttgagattt
     5581 cttcttcaga attattacta cgtgataaga aattgcttgt ttcaatcctt tctatcgcaa
     5641 tagcaaggtg gatagctaaa gtttgaaatt cataatctgt aaatttcaat tgtgtcttat
     5701 tcatgtatcg attaagcaat tcggctactt ttttcaatat gtccttattt aagaaattat
     5761 tcgcttcacc aatgaaattg atattcaagt ctaagagatc tgttttactg ttagatgctg
     5821 taaagccacc ccaataatag ttcacaactt ctgacatcaa ccttctttta cttttttcat
     5881 cagctttgat gcgcacgcca tttcgatctg aaactaagga catcccatgc ttgtttagtc
     5941 tttttctaac atctttcatt gtgctttcta ccgtcgacct gctcacgtat aattcgtcag
     6001 caagattttg aatctttaga gaatcattag tattcaataa tttagcaaac acataagtta
     6061 gacggtcctc cggagagtct gcgatattga ttttatgact ggataataaa tttcgaagtg
     6121 tttctttttt tccaataagc gaaacgcctt ttttaggttg aaccaacatt tgtacatctg
     6181 gcattttttg gattatttct ttcaaaatta aaatatcttt gctaattgtt ttcgtagata
     6241 catataattg ttctgataat tctttgtaag ttgtgtgctc attttcaatc aaaaagtata
     6301 gtattttcat ttgccgttcg ctaaattcga tgcccattat cttcacctcc tgaatacagc
     6361 ttaagtgtac aattattatc tctattttca tacagaagaa agttcttgat taatgtagaa
     6421 gtttctaata ttcttgatta tttcaaatca ataaaaaagc ctgcaatatt tatatcacag
     6481 gcttttttct ctattaaata tatataagat aatacatttt tctactagtt ctcactaatc
     6541 ttgtataatt aaaccctccg caacttgacg gagatggaga agatgccccc gtctaatcta
     6601 gcggtcatct ctcctcccat tccttccacc aatttttttg acaggtaaag cccgattccg
     6661 ctagattccg tatttgtgcg gctggtattt tcggtataaa aacgctggaa gactttttca
     6721 acagaggttt gcgttcctgg tttgatagga ttggccactg ttaatcgaat gaatggatcc
     6781 tcttttgaaa atgtcacctt tgcttgtcca tctgcataac gcaataaatt ctgtatgata
     6841 ttttgcagca ctcgcagcaa catgagcgga tctgctaata cagttgtatt tatcgagtgt
     6901 tcagcgaacg ctacctcgat atttttctca gaaaattgtt cataaaaaga gaaaaatact
     6961 tcttccacct ctttagaaaa agaaagcgta tggagatcca tttctttttg cttcgtctct
     7021 attcgagcaa gatcataaaa ctgatccgtc atactgagta atcttgcaac agattgtttg
     7081 atgatcgtta aatacttctc ccgtttctcc gtatcctcta cctgttccag taattgtaca
     7141 taaccgttaa tagcagtcaa aggagttctg agatcatgag aaaggccgac gatcgatagt
     7201 ctgatgtttt cttccatttc ttgaaatgct tgtccttttt gttcattttc atccaaccat
     7261 gtattcagtg catctactag gtccattaga ttcttttctc gaaattcgac tgaaagacga
     7321 ccaccatatt cttgtcgttc agaaagagtg gcgattttct tagttaatct cttgatttcc
     7381 ctttgcagga agaaaataaa acttcccata aagataacaa cgagaagcaa cagccattta
     7441 atcaaatcta aaccccactc cccacaccgt atgaatatac acagcatgtg cctttgctag
     7501 cttccctcga agattggaaa tatgcatatt cactgttttc tcactatcaa aatatggttc
     7561 ttgccaaact gtttcataaa gatttgctcg cgaaaatact tttttcggat gttctaaaaa
     7621 aagcttcagc agatcgaatt ctcttcctgt caaatggaca gccacaccat tcacatttac
     7681 atccctcgac tctttgtcca gtaagatttc cttgtaatag atgcgttgcg acagatcttt
     7741 tacttggaca gaagtctgat gacgaagttg gatgccgatt cgtaacaaaa gttcttcgat
     7801 atcaaatggt ttcgtcacat agtcatccgc tcctgctgcc aacaaggaaa ctttatctct
     7861 ttgatcgctt tttgctgaaa gaacgatgat cggaacattc gatgtctgtc ggatttctgc
     7921 taacaatgct tcacctgaaa gccctggaag catgagatct agaaggatca aatcgacagg
     7981 atatgtgctt aacaataatt ttccttccgt acctgaataa gcatcaagaa gtttgtatct
     8041 ctgttcaaga acctcctcca gtaaggaatg aacagcttca tcgtcttcga taatcaaaat
     8101 agtttccagc aggaccacct acttaatgtc agatttttgg aaaataaaca ttcctagagc
     8161 tgatgatacc acgattgtta ctagactgac cagaatcatt ttcggtgctt gcgaataagt
     8221 tgcttgttgc gcaaagtccg tatacatgag ccacgtttcc gtaaaccact ccgttagaaa
     8281 ttccaagcct ttgatttttg cgaaaacacc gggaatgaag gccgttccca tgatataaag
     8341 aaaagaaaga agcaaactga cactggtact tctagtgatg aatgtcaaag ccactggaac
     8401 agataatgtc cctaatgtca gcagaagcaa tacaggaaat acttttagaa cataatttcc
     8461 atagtttttg attccctcaa cttcaccaaa tccgaaagca agtgtgtaag caagcatggc
     8521 tagtcccgtg atcaaaacca cgccaagtgt tgtgatcaaa agcgtaacga gaagtttcga
     8581 cacataataa gtcattcgat tccgtcctaa agataacgta ttgcgaatcg taccgttttg
     8641 aaattcttcc ccaatataaa agcacgcaaa tgctgcgaaa gccagaagaa tccatgcagc
     8701 cattttttct accattaaaa tccctgttgc accgttgatg actgtatccg ttgcttggtt
     8761 taattcattg ccgtattttg tatgcatata ccattcgttc caagaataaa aaagcggaag
     8821 tacgaccatc agtcctagtg agacccacat tttacggctc ttccatactt ttattttttc
     8881 agcagttaat agattaaaca tgtggattgc ctccagttaa ttgtaagaaa taatcttcca
     8941 gtttttgccc aactaggtga ataccatcca caggaacttt tgcttctacc aatgcttgat
     9001 tgatggctgc tacttggtca attccttcaa aaagctggat aacacggcta gattgtacaa
     9061 aataatccct ataaccaaga gagtcgagaa ccgttactgc ttgttccgtc gccgaagtgt
     9121 caatttttat aaattgacga gattcttgtg ccaattcttc tttcgataat tctttgatca
     9181 agtgtccttc atgcaaaata ccatagtgag tggcgatctg tgacaattca tctagcaagt
     9241 ggctagaaag caagacggtg atgccccgtt cttttaccaa acgttggatg atttctcgca
     9301 tttctgccat ccctgctgga tccatcccat tcgtcggttc atccaaaatc aaaaattctg
     9361 gattcgtgat cagtgcattt gcaatagcta aacgctggcg catacctaaa gagaaattct
     9421 ttgctttctt tttccctgta ttttccaatc gcatcatttt cagcaggtct tcgatttctc
     9481 tatccgacac accgccgttc gctgcttgga tacgtaggtt atctcttgca gttaattctg
     9541 gataaagagc aggggtttca atcgattgac ccatacgtcg tcttgctgcc tgcaaatccg
     9601 tcgttccaaa taattcgatc gaaccttcat caatcgagat caaacccata atagttctca
     9661 taaaggtaga cttacccgct ccattttccc caatcaatcc atagatcatt ccacgtttga
     9721 tttcgattga tactttatct aatgctttgg cagcaccgta ttttttggtt ataccagttg
     9781 ctttcaagac cgtttcattt ccaaaatcca tacttatcct tctttcttat tgatgagtga
     9841 agtatagttg acgggtataa agaaagcagc cagaaatgta taaagaaagt gtaaattttc
     9901 caattgaatg tcaaaaaaaa caatcggaca atacgtgtct ttcctattat caggcagttt
     9961 gttatacgct tgtatcagtt tcctgattct ttcttttcga ccaaagtcgt ttctctgtat
    10021 tcctcttcac tgaaaatttt tgcttctttc agatcaagct tgagtaattt taccagaccg
    10081 gttataagga taactatagc aagactccct cccagaaaaa ttagactgtc tcgcagacgt
    10141 ggttgcgttg tcttccaatc aaagagcgca ccaaacagtg ctaatccaag tggactggcc
    10201 accatggtaa tcgttgttat cgttgacata agtcgtcctt gtatatgcgt tggaacttct
    10261 cgctgaagcc agaccatata aaaagcaaaa gaaaacatta atccttaaaa acggacttat
    10321 gaaaaataat acatgccgtt tgggatttac aattagctac caaataagag gttatgacaa
    10381 aagacttttc acaatccctt cttccgaata aacggtgttc tcctgccaac tcctcggtca
    10441 caagtcacaa tatttatcaa acatgagaag caatttttgt aaattccttc ttatttcttg
    10501 aagctgacca cttctgtcac aacctcagaa gcggataaat aatattgttt gatgatactt
    10561 tatctttttc ccgtttgttt atagatcgaa tgatattata gaccaagaat cctacctatt
    10621 aacatagcta aaaacacaaa aaaggcactt tccaaattgt ggaaagtgcc agaaaagtta
    10681 ggattaaggt aactgcgctc agcattgctt cgcccagtac gattctcgta actcgtgaac
    10741 tcgaacgata atcgcaatta acgttttgag aactgtgaag ctttacgggc tttcttaaga
    10801 cctggttttt tcttcgtatc attgaataag tttgaaaaac cttgatttat aaaggttttc
    10861 ccgtaaatat attttattat atttgattaa atttgaaatg tttgtgttct aaaatgtgtt
    10921 ctaatttaaa ggcagtatat ttcgtatttt agattaaatt tttaggctca ttcattctca
    10981 aatccattct attcagtcaa taaaaatttt aaaaagactt tataaacaaa gatgttcaag
    11041 cgagcatctt atttttttgt gcttaatttg aaaggagaac atttcatgta taaaaaacaa
    11101 aaaaagaaat tatgtgtatt aactattttg agtttggctg ttcaagcaat tgttggagca
    11161 attgctccta cgattacttt tgctgatgaa attacccatc cacaaacggt aaccgtagaa
    11221 ctagatttag cacatcaata tgctgttgaa ggaactttta gtgatggtcg tccaatgtca
    11281 gaagtcactg ttccgcatta tgcagtgtac aacggcttta aacaagatat tttctgtatt
    11341 gaaccaggag tcccgattta caatgagttc actcctggct atgagaaaaa cccgttacca
    11401 gatatgtccg ataaagccaa attagtttcc gttttatgga aaaaagtagg tacagatgca
    11461 gatactcata ttgtggcaca gaagatgatc tggcaagaag ttaatgggta tacccttcac
    11521 tcaataaaac gatcagatgg tagtgccgta aatatcgcag ccattgaggc aaaaataaat
    11581 caagccattg acgattatca gaagaaacca accttccata atagtacggc taaaacagta
    11641 ttagggcaat cgactactgt gacggatacg aataatctga atttgtcaga gttcgatgag
    11701 gttgtggaaa atgccgcaaa cattgattat cgtgtgaatg gtaatcagtt gataatcacc
    11761 ccaaatgcca attcaaaaga aagtggtgtg ctgacgctta agaaatctgc tggtaccgga
    11821 acacctgtcg cctataaaaa ggctggccaa cagactttaa tggccggggc gattgataaa
    11881 cctaatacct acacaatcaa aattgatgtg gaaacggagg gttctttaaa gatcaaaaaa
    11941 gtcgataaag aatcgggtga tgttgtacca ggaacggttt tccatttaga ctttggaaaa
    12001 actttacctg caaaagacgt gactactgac aaagaaggca ttgctacatt ggatgagatt
    12061 cctcatggta caaaagtaac cattaccgaa aaatcagtac cggcgcctta tacgattgat
    12121 accaccccca tgactaccac cattaaagca ggcgaaacca tttacgtgac ttcaaaaaac
    12181 gcacgggaaa aagggcaaat cattctggat aaaagcggag ttgaaacagg gagtgatctt
    12241 tggaatgaca attattcctt agctggtaat acgtttgcca ttcgcaaaga cagtccaacc
    12301 ggtgaaatcg ttcaagaaat gaccacggat gaaaacggtc atgcggaaac accaaaagag
    12361 attgctaatg cgttggaatt gggaacctat tatgtgaccg aaactaaagc tagtcatggg
    12421 ttcgtgaata cgttcaaacc tgtaaaagtc gaattgaagt atgccaatca aaccgtagcc
    12481 cctgttacca gtaacgtaaa agggcaaaat caagaagtca ctggcgaaac cactttgacg
    12541 aaagaagaca aagatactgg ggataaagca caaggtaagg cagtctttga gggtgctgaa
    12601 tacaccttgt ttactgctaa ggatggaaaa gctgttaaat ggagtgaggc ttttaaacca
    12661 gagttagcga agggaacgaa aggttctgat gaaacggtca ctttgacttt agacgaaaag
    12721 aaccaagttg ccgttaaaca tttagctatc aacgagtatt attggcaaga aacaaaagcg
    12781 cccgaaggat atagccttga tgaaacaaag tatcctgtat ccatcaaaaa ggtggatgat
    12841 agcgaaaaaa atgccgtgat tacccgaaat gttacggcaa aagaacaagt gattcgcttt
    12901 ggctttgatt tctttaaatt tgctggatca gctgcgggca ctaccgaaac tggcttcaat
    12961 gatctgacct ttaaagtgtc gccattggaa gggaccaatg aaatcacagg tgccgaagat
    13021 gaagcgacca cagcttataa cgagcaatta gggtttgatg gttatggcaa gtttgaaaat
    13081 cttccttatg gggattattt acttgaagaa gtagaagccc cagaaggttt tcaaaagatt
    13141 acgccattag aaatccgttc tacttttaag gaaaacaaag aagactttgt gaagagtgag
    13201 tatgtcttta ccattaccga acaagaccaa aaacaaccaa ttaaaacggt gaccgttcct
    13261 tacgagaagc tgacgaacaa agcattttct gttagtttga accgtttaat gctctatgat
    13321 ttgcctgagg aagaagatag tttaacttct cttgcgactt ggaaagacgg aaacaaagaa
    13381 ttaacttctc tggattctac cgagctagtt gataaattga gttacaatct acatgaaatc
    13441 aaagaagact ggtatgtggt agcgcaagcc attgatgtgg atgcgacaaa agctgcccaa
    13501 gaaaaagatg aaaaagccaa accagtggtg attgcagaaa caagcgcaac gttggcgaac
    13561 aaagagaaaa caggaacttg gaaaattcag cataaattaa ccgccgaaca agttttaaat
    13621 aaaactattg tcttattcaa ctatgtgtat gaaaataagg aagcttttga agcaggcgat
    13681 aagccagtag cgaaggatgt tagcttgaat aaccaagccc aaactgtcag ttgtacagtg
    13741 gaacaccatg tttctatcca aacaaaggcc cacctagaaa acggttctca gacctttact
    13801 catggtgatg tggtggatat gtttgatgat gtgtcgatta ctcatgatgt actggatggc
    13861 tcaaaagaag ctttcgaaac aattctgtat gctttattac cggatggtac aaacaaagaa
    13921 atttggaaat ctggcaagat tgattatgaa gtaaatgata aagaattcac caaaactgtg
    13981 cttgcgaaaa aagtagatac cggaaaatat ccagaaggaa ccaagtttac ttttgcggaa
    14041 atcaattacg ataaagatgg gaatatcaat ggaaaacaca atgaagattt gaaagaaaaa
    14101 tctcaaacct taacaccaaa agaagttcca accatactaa gtacgccgaa acaaccggaa
    14161 acaccaactg ttccaagtgg ctctcaagaa tctagtccca cagtgaagac cttccctcaa
    14221 accggggaga aaaattccaa tgttttactg ttcattggtt ttactttgat ttttgcgacg
    14281 gcgggctatt atttctggaa tcgccggaac taaggtgatg agatgataca agtaaatcct
    14341 aagaaccaac gtgcgcgacc gcctcctaaa aaagtagtcg cgctattttt gtatccaaaa
    14401 ctaacaatga aaagaggaaa agaagatgga attgaaattt gtcgtgccag atatggctga
    14461 aacctttgga aagttaagtt atgccggtga aggagaaatt ataacagaag gatatggacg
    14521 gaatactacg gtgattggtc gcagttatca tttgtattcc agtaagcaac gggccgatga
    14581 tattgaggtg gtagtagctg cggaagccgg tgaaaaggat tttgatcaag accaaccgct
    14641 aaaagccgtg aatccccatt tggttgccaa aggctatgag attgagaatc gtgggtttac
    14701 tgattacgtg ttgtatgttg atgatttagt gaaagcatag gaggaaaaga agatgagatt
    14761 aacagaaggt attgtcgtag attcaggttt aacatttggg aagttgcgtt tttccgcatt
    14821 acgccgagaa gtacggaagc aaaatgagga tggaacaatt ggtaatgagg taaaagaacg
    14881 aacctataat ttaaaatcct ctgcacaagg gcgaatgatt caagttagta tcccagctaa
    14941 tgtaccctta cgcgagtttg cttatgatgc agaggtagag ttggtcaatc caatcgtgga
    15001 taccgttgcc aattatgttt ttcgagaagg aactaccgtt aattggttca ttaaggcgga
    15061 tgatttagtc ttgaagcggc agccgaatca aggaaatcct acgaaccaaa atgaaagaaa
    15121 gaaataggtg aagattatgg aaattaatgt tgaacatatt ttagattgtc tcgatcaata
    15181 tggaaaaggc gagctgactg aggaacaact cgttaaagca ttaacctctg atgaaaaaat
    15241 gtttctgatt atacatcaag gtttacttgg agaaaatagc gacagtgaag aagactctga
    15301 tgtactagac tggttaggag aagaagagtc tttctttata gttgtcgaag taaatgaaaa
    15361 cttatgtcgg caggcagaat ccattttgga agaaattggt gtggagatgc cggatgctat
    15421 tgaaggcttt ttaaatcaat tggttgaaac gaaacaactc ccctcctcgt agcagtaaat
    15481 gactagcata actttttgaa attgcctaat gaaatcctat tgggcttttt cgattggagg
    15541 tggaaaccat caaaatgtac aaaggacatc gcatacgcgc aggagatcag cacttggttt
    15601 atcactttgt tcttggctgg ctactcgctc tgttcatcgg ttggatgagc gtcttttatt
    15661 ttcaagagtt tagacaattt gatatttcta agctatcact ttctactatt gaaatcgttt
    15721 ggtctataaa agatttagta tgtctgcttg gaagtctcgc tttttcagga gctatgatat
    15781 tgctttatat tcactttttt cttgatcatt ggcgaagctt gtggcatcga caaaagctag
    15841 ctcggatgat tctagagaac cattggtatg aagtgaaaca aactcaaagt gaaggctttt
    15901 tcaaagattt gaatagtagc cgaaccagag agactatcag ttacttcccc aaaatctgtt
    15961 atcgaatgaa agatggatta ctgtctattc gcgttcaaat ttcacttggg aaataccaag
    16021 atcagctttt aaaattggaa aagaagttag aaagcggact gtattgtgag ttagtagaaa
    16081 aggaactcaa agactcctat gtggagtata ctttactgta tgatatgatc gccaatcgaa
    16141 ttgggataga cgaagtagtg gcagaaaatg ggactctgcg attgatgaaa aatcaagtat
    16201 gggcttatga ttctttaccc catatgttaa tcgctggtgg cacgggtgga gggaagacct
    16261 atttcctcct caccattatc gaagcactat tgaagtcaga tgcagaattg tttatcctcg
    16321 atcccaaaaa tgcagattta gctgatttag gtacggtgat gcctcatgtt tattcacaaa
    16381 aggaagaaat ttctgcttgt gtggaagatt tctatgaacg tatgatggct cgtagcaagg
    16441 caatgaaaga aatgtctaac tacaaaacag gagagaatta tgcctatctt ggacttccac
    16501 ccaacttttt aatctttgat gaatacgtgg cttatatgga gatgttaacg acgaaagaaa
    16561 gtgcggtgat tttgaataag ctcaaacaaa tcgtgatgtt gggtcgtcaa tctggtttct
    16621 ttctgatttt ggcttgccaa aggccggatg cgaaatattt aggagacgga atccgcgatc
    16681 aattcaactt tcgcgtggct ttgggtcgta tgagtgaact cggatattcc atgatgtttg
    16741 gggaggtcga aaaaaacttc tttatgaagc acatcaaagg tcgaggttat gtggatacag
    16801 gtggcagtgt gattagtgag ttttatacgc cacttgtacc aaaagggtat gatttcttag
    16861 attcaattag taaattagaa aagatgaatg attgagtgat cttcaatact gactttatac
    16921 ttcaagtaac gtataatact tttatatagt aggaagaaag gggataatta atatggaaag
    16981 taatgattct tatttagagt atgcaaaagc aaataaggat aagtttattt cttatattat
    17041 caaagggaaa tcccccgaag ttggacgaga cgcagttttt atggcaggga gtcctggggc
    17101 tggaaaatca gaggttgcca ttggattagc tgagaattat gataaccatg tgattattga
    17161 tgcggattac tttcgcaccc agtttcctga ttataatgga aaaaactcta gtctctttca
    17221 aaaagcatct tcttggttgg tagagcaggc atttagatat gtcttagatc atggctattc
    17281 ttttattctg gatgcaactt ttgcaatatt aagtgctgaa aaaaatatct cgcgggcgtt
    17341 gaaaaaacat tttcgagtga cgattttcta tgtttatcaa gaccctaaaa tcgcttggaa
    17401 atttacacaa gaacgtgaaa ttgcagaggg acgtcatgtg ccgaaaaagg catttattaa
    17461 cgcttttttc aaatcaagag agaacattgc aaaagtaaag agtaaacatc cagatgtatt
    17521 gcttcatatc ataataaaag attatacgaa taatatttcc gaagttcatt atgctacgga
    17581 taatctagaa ttatttttac cactagggta tagtaggaaa gatttggagg ttcaattaca
    17641 tgactgagaa agaacaagta acgaaaattg taaagaaata caataagagc attgcggatt
    17701 tatcggaaaa tgctactgca aaagagttca aaacggtgat taaatatgta gccgatcagg
    17761 ccaatgaaaa acaacggaaa ttagttggat taaataaaaa atagtaggac aaattaataa
    17821 tgtatgagcc aagagcaaaa tgttcttggc ttttttcttt gcaatgaatg aggtaatgta
    17881 tgagattttt agatttattc gccggtattg gtggctttcg tttaggtatg gaacaggcga
    17941 gccatcattg tatcggtttt tgtgaaattg ataaatttgc tagacgtagt tataaagcaa
    18001 ttcatgatac aagtaaggag gtggaaatgc atgacatcac aagtgtatca gatgagttta
    18061 ttcaatccct cggaccagtg gacatccttt gcggcggatt tccgtgccaa gctttttcaa
    18121 ttgcgggaaa gcggcaaaga ttttccgata ctcgaggtac tttattcttt gaaatcgctc
    18181 ggttcaccac tcttctccga cctaagcttt tattccttga gaacgtccga ggattgctca
    18241 atcacgaagg aggggctacg ttcgagacga tcctccgaac gctggatgga ttggggtatg
    18301 acgtggagtg gtaggtgctt aactcaaagg cctacattcc ccaaagccgg gagcggatat
    18361 tccttatcgg acattctcga gacgcatgta ccgaacaagt atttcctatc attggaccgt
    18421 cttcaacatc tgatcaaaac atcagaaatt tggtaaatat caatccttcc aatcgaggaa
    18481 tgggtggaca agtctatggt tcagatggcg tagcgccaac cttaacaggt gatgaaggaa
    18541 tcaaaatcgc actgccagca agggatggaa tctctgtagc aggactatta ccgggaaagt
    18601 ttgaacaagg taatcgagtt tatgaagtta ctggaaccgc ccccaccttg tcaacgaaac
    18661 aaggtggtac aaaaatcatg attcgaaaaa atgaaacaga atatcaagag gtgaagccag
    18721 aagacagtgt gaatctggct tttcctaatt ccacaagtcg ccgaggtcgt ctgggaaaac
    18781 agagtgttca taccttacta acaggagacc aacaagcgat cgtcacggat cagtatcaaa
    18841 ttcggaagct aacacctaga gagtgttggc gacttcaggg ctttcccgat tgggcttttg
    18901 atcgagattc tcaagtcaat tcagatagtc agttgtataa gcaagccgga aactctgtaa
    18961 cagttccggt catttttgat attgccagaa gattaaaaga gttggatgta catggtttat
    19021 gatgagttga ttggagaaat ttattgggtg attggaaaga ttcagtcaga cccagaatta
    19081 gaagaacaat tgcgacggtt aaaatttgat attcgcaaga atggtgtcaa agtcgctggc
    19141 gatccatatt taatgaatga ggagacggat gctcgaattg aaataaatca agtaattgca
    19201 gaatttgagc ggattgcgga ccaagcaaaa gaaccagata ttcgtcaata cttgttagaa
    19261 atgaaagcag agttggaagt aaatggtatt acagatgagt aacctaaacc aacacaactg
    19321 actgttaggc gctgaccgcg gaggattcgc gaagcagaaa tcctccgtgg tgctgtgcct
    19381 gttcatggac ggcgttagcc gaccatgatg tctcaccccc gtatctaaca ggggggtaca
    19441 aaaacacaaa aaataagtaa gcaaccatta agaatgcttg ttgtatcaaa gatagtaagt
    19501 aatttgtata ggtgttaact catttgaaaa agttaacacc ttgaatttgg aggtgagaac
    19561 ttggcacaac ggaatttaga ttatcggcta ttaaaagacc gacgcaacga atacggcgtt
    19621 tcacaaaata aattggctac ggcttgtgga cttagtcggc cgtacttaaa tcagatagaa
    19681 aatggtggcg taaccgcatc tataaaaacc atgaggaaaa tttttgacca actggaaagc
    19741 tttaatcccg atttgccttt gacgttactg tttgactatg taaggattcg ctttcccaca
    19801 acggatgcac gaaaaattat tcaagaaatt ctccatttga aatttgagta tatgctccat
    19861 gaagattatg ccttttactc ctatcaagaa cagtatgtca tgggtgatat tgtggtgatg
    19921 ttgtctcatg aagaagataa aggtgtgctt ttagaattaa aggggcgagg ttgccgacaa
    19981 tttgaaactt ttttactcgc tcaaaagcgt agctggtacg actttttcga ggattgtcta
    20041 aaggccggtg gcgtgatgaa acgcttggac ttggcaatca atgatcgagt ggggctattg
    20101 gatatacctg agctaacgaa aaaatgtcag aaggaagaat gtatctcctt gtttcgtacc
    20161 tttaaaagtt atcgttccgg ggaactttta aaggctgatg aaaaggacgg catgggcaat
    20221 accttatata tcggcagtct caagagtgag gtttatttct gtttgtatga aaaagactat
    20281 gaacaatata tcaagttagg gattccatta gaccaaactg agacgaaaaa tcgttttgag
    20341 attcgactga aaaatgaccg tgcttttcat gcgattcaag atttgttaaa aggtcgtagc
    20401 attgagagca ccacgttttc cattatcaat cgttacttgc gatttgcgga taaagtagaa
    20461 ggtaaaaggc gaaccaactg gcccttgaat gaacaatggg ggcgttttat tggacggaat
    20521 cggaaagaga ttcaattaac ttccgagcca aaaccgtata ccattgaacg aaccttgaat
    20581 tggctaggcc gacaagtcgc ccctacgtgg aaaatggcta aggagttgga tcgattgaat
    20641 cggacaacct atatccagga tatggtacag aatgcacgct tatcagaccg acataaaaag
    20701 attttggagc aacaaagtat ggcaattgaa aatttgatta tatgaaaaga ggaatcgtca
    20761 tgggaattgt aaaagatatt tttttggtat ttggtggcag tatgcttggt gttactgtga
    20821 tgtgcttgat gaatgcaagt gcagcagcgg atcgagcaat ggaaaagaaa ggaaagtgac
    20881 aagatgaatt tcggacaaaa ttt
//
LOCUS       DACDSY010000035        20666 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDSY010000035 DACDSY010000000
VERSION     DACDSY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.035"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            352..1041
                     /locus_tag="IWK44_001104"
     CDS             352..1041
                     /locus_tag="IWK44_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9549643.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="IWK44_001105"
     CDS             1028..2236
                     /locus_tag="IWK44_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9549644.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="IWK44_001106"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="IWK44_001106"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP9549645.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="IWK44_001107"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="IWK44_001107"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP9549646.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="IWK44_001108"
     CDS             complement(6339..7034)
                     /locus_tag="IWK44_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP9549647.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="IWK44_001109"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="IWK44_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP9549648.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="IWK44_001110"
     CDS             complement(8233..8424)
                     /locus_tag="IWK44_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP9549649.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="IWK44_001111"
     CDS             complement(8445..9335)
                     /locus_tag="IWK44_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP9549650.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="IWK44_001112"
     CDS             complement(9322..10089)
                     /locus_tag="IWK44_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9549651.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="IWK44_001113"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="IWK44_001113"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP9549652.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="IWK44_001114"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="IWK44_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP9549653.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="IWK44_001115"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="IWK44_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP9549654.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14705..15043
                     /locus_tag="IWK44_001116"
     CDS             14705..15043
                     /locus_tag="IWK44_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549655.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="IWK44_001117"
     CDS             complement(15211..15969)
                     /locus_tag="IWK44_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9549656.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="IWK44_001118"
     CDS             complement(15987..16805)
                     /locus_tag="IWK44_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9549657.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="IWK44_001119"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="IWK44_001119"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP9549658.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="IWK44_001120"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="IWK44_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP9549659.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="IWK44_001121"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="IWK44_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP9549660.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="IWK44_001122"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="IWK44_001122"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP9549661.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5921 a         4215 c         3796 g         6734 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggcc
//
LOCUS       DACDSY010000036        20490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDSY010000036 DACDSY010000000
VERSION     DACDSY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.036"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            86..1165
                     /locus_tag="IWK44_001123"
     CDS             86..1165
                     /locus_tag="IWK44_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9549662.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            complement(1362..2063)
                     /locus_tag="IWK44_001124"
                     /pseudo
     CDS             complement(1362..2063)
                     /locus_tag="IWK44_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(2077..3001)
                     /locus_tag="IWK44_001125"
                     /pseudo
     CDS             complement(2077..3001)
                     /locus_tag="IWK44_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3118..4197
                     /locus_tag="IWK44_001126"
     CDS             3118..4197
                     /locus_tag="IWK44_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9549663.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            4229..4822
                     /locus_tag="IWK44_001127"
     CDS             4229..4822
                     /locus_tag="IWK44_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9549664.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(4932..5159)
                     /locus_tag="IWK44_001128"
     CDS             complement(4932..5159)
                     /locus_tag="IWK44_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9549665.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(5262..7850)
                     /locus_tag="IWK44_001129"
     CDS             complement(5262..7850)
                     /locus_tag="IWK44_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP9549666.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(7870..8529)
                     /locus_tag="IWK44_001130"
     CDS             complement(7870..8529)
                     /locus_tag="IWK44_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9549667.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(8578..9081)
                     /gene="trmL"
                     /locus_tag="IWK44_001131"
     CDS             complement(8578..9081)
                     /gene="trmL"
                     /locus_tag="IWK44_001131"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP9549668.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(9302..10168)
                     /locus_tag="IWK44_001132"
     CDS             complement(9302..10168)
                     /locus_tag="IWK44_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9549669.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            10277..10918
                     /locus_tag="IWK44_001133"
     CDS             10277..10918
                     /locus_tag="IWK44_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP9549670.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(10980..12341)
                     /gene="mgtE"
                     /locus_tag="IWK44_001134"
     CDS             complement(10980..12341)
                     /gene="mgtE"
                     /locus_tag="IWK44_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP9549671.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(12359..13258)
                     /locus_tag="IWK44_001135"
     CDS             complement(12359..13258)
                     /locus_tag="IWK44_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9549672.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(13260..14057)
                     /locus_tag="IWK44_001136"
     CDS             complement(13260..14057)
                     /locus_tag="IWK44_001136"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP9549673.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(14035..14739)
                     /locus_tag="IWK44_001137"
     CDS             complement(14035..14739)
                     /locus_tag="IWK44_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP9549674.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            14870..15445
                     /locus_tag="IWK44_001138"
     CDS             14870..15445
                     /locus_tag="IWK44_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP9549675.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            15563..16240
                     /locus_tag="IWK44_001139"
     CDS             15563..16240
                     /locus_tag="IWK44_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9549676.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(16319..18127)
                     /gene="pepF"
                     /locus_tag="IWK44_001140"
     CDS             complement(16319..18127)
                     /gene="pepF"
                     /locus_tag="IWK44_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP9549677.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(18303..19301)
                     /locus_tag="IWK44_001141"
     CDS             complement(18303..19301)
                     /locus_tag="IWK44_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP9549678.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(19527..20180)
                     /locus_tag="IWK44_001142"
     CDS             complement(19527..20180)
                     /locus_tag="IWK44_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP9549679.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5939 a         4456 c         3647 g         6448 t
ORIGIN      
        1 agccatttaa aataaaaaaa acgccacgtg aattccatgt tatattgaag ttaccacaac
       61 tctcaataga caaggatgaa ttcacatgac gcaaacccat gataacacag tttcacgcaa
      121 gggaaaacat ctttcctact cagaacgttg ccaaatcgct atcctgaaaa aggaaggcta
      181 ttccaaccgt caggttgcca aagtgctggg acgtgttccg caaaccataa acaatgagat
      241 tgatcgcgga accatcaccc agttaaaacg ccaaacgcaa aatggaaaag tgtatgacac
      301 ctactattct gtttatgacg ctgatgcagg acaggccttc tatgaaaagc aacgactgaa
      361 ctgtggcaga cgccccaaat gggcgaacac aaatgccttc attgattggg cagatgacaa
      421 aatgttaaat gaaaaatggt cgccggacgt cgtggtcgga tttgccagaa aacacgagct
      481 gttcgattgt gccatcattc ctggcacaac cgctctttat caatggattg accgcggaat
      541 catgcgaaca aagaacatgg acttgcttga aaaactgtcc cgtaaaatca aagcggtccg
      601 cccaggacac cgtcccaaca agcgtgttct cgggacgtca attgaagaac gccctaaaga
      661 agttgaaaca cgggaaacgt tcggtcattg ggaaatcgac acggtagtgg gaaacaagac
      721 caagtcggat gccgtgctgc tgacgctggc tgaacggcag acacgctttg aagtcatcct
      781 aaagttgaac gggaaggaca ccgaatcggt ggaccaagcg gttcaacagc taagagaacg
      841 ggctggcgct aacttcgatc aattatttaa gaccgttaca tccgacaatg gctcggagtt
      901 ttctgggcta cacgatgctc tgcaagatgt agctgacgtc tatttcagcc acccctatgc
      961 ttcttgggag cgtgggacga gcgaaaacca gcacaaatta attcgccgtt tcgtccccaa
     1021 gggaagcccg attagtgagg tgagcgagaa ccagattttg cggatccagc gttggatgaa
     1081 cgattacccg cggaaaattt tgggttatgc gacaccacat gatacattcg tccaggcatt
     1141 caagcaagaa cggttggtgg cttgacccat tattccttga taacgatcct cactcgggac
     1201 atggcttcat gtctggaaag ctggcctggg gccagaatcg tctgaagact atcatacctc
     1261 agttcattat caaggcctca ctgatttaga ataacgaggc agttacggta tcaaacaaat
     1321 gagttacctg tagtggctaa cttaaacttg aaatttacgg acgtaaagct tgctgccaaa
     1381 ttagcgattg aaactaacga ttgctttcta ggaatcaagg acaagcgaat accatttgac
     1441 gattgatttg cttgctgatc ctgtacattg cggacccccc acatcgtccc gtagagaatc
     1501 gccattccaa tcagagtgaa gaaataaaaa cttttgaaac tcatattacg acctgcatga
     1561 acttcttgaa aagatacttc ttgttcaaaa acagtcatga tgggcgcgag atcaacgttc
     1621 tccacttctg ccactttact cgaaattata ccaatacgct gtaaaaattg atccatgacg
     1681 tttttcatca acgtctgact cattcctgct tgacccacat ggagcgaaac agatgaacca
     1741 tcaatgagat aaaaaccagc caaatcatct gcatttagct tttcattcgc ttctacttcc
     1801 gatagttcct gtacttcaaa taatggttta tcattgactt cgacttgttc taagactgtg
     1861 gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta caattccagt attaatcgtt
     1921 tcgagattgc caccttgatc aatctcgcca aaggccgcca taaacatcaa tcctaaaaga
     1981 attggaaagg ccaatgtcca aaacaacatg gatttatttc gcagcagtat ttttgtgcga
     2041 tatttataaa aatgccaaaa cattatttcc cctcctctag tctctcaaat ccttgccagt
     2101 aatctctaaa aagacatcat tcaaggacgc ttctttggtt gctatttttc cgtaactgat
     2161 cttttcttct tgtaagattg taagaatcgg caacaatact gtatttagtt cagtagttgt
     2221 tacttggagc tgaccttttt tataagcgat tccatcagaa gctattgttc gctgacaaca
     2281 cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc cgccgtccgt accatatctt
     2341 ttagagattc tttggttcct ttagcaatca cctgtccttg atccatgatc acgatctggt
     2401 cgcaaagaat ttctacttct tccatgtaat gtgttgtata gacaatggtt gctccttgtc
     2461 gattcagctc cttgatactt tctaaaattt tattccgact ttgggggtct acagctactg
     2521 tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat cccacatgcg atgttcaacc
     2581 gacgtttcaa gccaccacta agttctcctg gacgaaacga aacatatttt tctaatccca
     2641 ctaattgaat cacctcttca atcaaggctt tccttttctc tttttcggaa acatataaac
     2701 cgcaaaaata atcgatattt tcttgcacac ttagttcgtc aaagaccgca acgtcttgag
     2761 gaacgacccc aatcattgcc ttgacagcat aattttcagg agtcattgtt gtcccgaaca
     2821 atttaatggt tcctttgtca taatttaaca aagaaagcat acagttgatg gcagtcgatt
     2881 tcccactgcc gtttggtcct aacagaccta agacttctcc ttcttgtact tgcagatcaa
     2941 aatgattcaa tgctacaaaa tctccatagc gtttgactaa tttatcaatt tcgataatca
     3001 tcatgaaaaa ctccttccaa atttcttgtt gatctgacta taataaagtg caagaaaaga
     3061 aactagtgac gattggctaa accacacatg acatttgtca caatgaagga gtctgctatg
     3121 cataaatctc ggctactcaa cgaaactagc tttctactgg ttatctcatt gcttttgctg
     3181 gtaagtcaag gattcgttgc tatgcaagtt atctatttat tgatcgccgt tttattcact
     3241 ggtgtcagtt atttactgcc tacaaagcca aattggattt tccaagttat tctatttctt
     3301 ttatgtgcct ttttacctga tcttgtcctg tttctgccag cgacgatgcg agtgatcgcc
     3361 cctgaagaaa gccaccttga ttacttgttt ttgttaggtt ttttgttgat ttcgacggtt
     3421 ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt tgctgttttt ttccttttac
     3481 ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa agtacattgt ctccaaagat
     3541 actcattggg aaaaacaaca agagttatcg gaacaaaata aagcattggc cctgtcacaa
     3601 gaaacacaat tagccttgca aattgtggaa gaacggaacc gaattgcccg agacatccat
     3661 gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa ttggtgcgct tgaaatgatc
     3721 aatcaagaac caaaactaaa agaaccatta gaaaatttaa gtgccaccgt tcacactggg
     3781 atggatcgga ttcgtcaaag tgtccatgac ctccatgaga catcgatctc ttttcaacac
     3841 tccttagagt ttctgatcgc tgattttcct tcttctgtta cgattgaagg aaacttgttt
     3901 gaaggattga atgaaacgca gcaaaactgc ttcttaatgg tcatcaaaga agccttaacc
     3961 aacagtatca aacatagtca tgccaaaacc gttgttttac attttcgaac gttgccggcc
     4021 ttttatcgct tgcaaatcac gaatgatggc accagcccac aaaaaaattc tgctcccggt
     4081 attggtttga aaacgatgcg tcaacgtgtt caagaacaaa agggacaagt ccatattttc
     4141 caagaaaacc agcaatttca gttaacgatc attttaccaa gggagggaca ccaatgataa
     4201 cagtagtcat tattgagatg atccatttgt gacgaaatcg ttacaaacca ttttagaaag
     4261 cacgaaagag atccgtgtgt taggcattgg tcattgtgcc aaggatgcgc tagtcttata
     4321 cgaaacccat cgtcccgatg ttttgctgac ggacatccgc atgcctaaac aaacgggcat
     4381 cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc atccttttgc ttttgacgac
     4441 attcaaagat gaagaataca tccgcgaagc attttccatc ggtgtcaaag ggtatttaat
     4501 caaacagaac ttgcaagcga ttattccttc tgtcaaatcc gcttataatg gacaggccgt
     4561 atttggtaat gaaatcatcg agactttcac tcaaatgatg aagaataaac cgacgattga
     4621 tagtcatgct tatgcttctt ttagtgaacg tgagcttgca atcatcaaag aagtagctgc
     4681 cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg agtgacggca ctgttcgcaa
     4741 ttacatcagc caattactag aaaagctaga tttacgggat cgcacgcaat tggctatcta
     4801 tttttatcag catttgcaat aagtcttatc taacacaaaa aaacctgaat gatgctcctt
     4861 cgcatcattc aggttttttt cacatcttct attcaattgt cccgcaaacc atttcctcac
     4921 gattgaaaga atcaaggatt cgcagggctg ccaggattcg ttggattgtt cggcgtgtta
     4981 ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt ttgattcttc acgatcgtct
     5041 cgttctacat caggactttc attcaatttg aattcttttt ccatctcatc taacttttta
     5101 aagtcattgc cagattcttt tgctaatttc gctaatttat cttcggtttc tttagccata
     5161 aagtaacgcc ccccttttga tttcagctta gcatatcaca ctgaaaaaac aaaagaaaac
     5221 gctaaccaac gatcattttt agctatttct catctttaaa atcaagcatt ttttgccggt
     5281 ataaaatcgt agggagaact gttttccata ccaatcatcg gatctactaa ctggttatcg
     5341 atcacttctg ctgtaagaac gaaatcttta gaaacagcag gttctacctc ttctttcttt
     5401 tcttctccaa caaatgaaga aaacagatct ggttccactg tttccattac ttggtttttc
     5461 gtttcattcg tcccactgtc atcttctaca ggtagctttt cttttacaac ccgtggctct
     5521 tttgtatagt tcccatcttc aaatggatca tttccgacca aagaatcttc ataagctgct
     5581 agtttggtcc ggagagtgat attcatttta tcattatcca tgatccttga tttcaacgta
     5641 gtcatccggc ttgctgattc attcgctaca caagtgacat aggcttgctc ttcccctaac
     5701 aaaatcaaca gttccttact gcgagtcact gccgtgtaga gcaaattacg ctgaagcatc
     5761 cgctgatatt gatggaccat gggcaaaata accatcttga attcactgcc ttgtgattta
     5821 tggattgagc aacaataaga aagagtgatt ttattccatt cgtttctttt ataagtgacc
     5881 tcatttgcat caaactggat aactaattca tccactttat cttcagaatc ttttgccaag
     5941 atgatacccg tgatttctcc catatctcca ttgaatacat tcaattcggg agtattgact
     6001 aattggagga ctttgtctcc tatccgataa acggtgtcgt tccaatgtac ttcctttttt
     6061 ccaggtttcc ctggattgaa tatctcctgc atcattttat ttaacgcatc gatccctgca
     6121 ggtccccgat acattggtgc taatacttgg atatcttgtg cacggaatcc cttatttttc
     6181 gctttttcca caattttaga gatcaagggt tcaatttgat aagcatcact tcggaaatag
     6241 gaacgatctt tttgattctt tgtgaaatct tttggcagac gtccttcttt gatctcatgg
     6301 gcaagaggaa taatactaga accatcacct tgccgataaa tctgattgag ttccattttg
     6361 ggaatttgtt cgatttccaa aagatcatgg agtacttgac caggaccgac agagggaagc
     6421 tgatctttgt ctcctactaa gataacttgc atattcgtcg gaatcgcttt gaataatgtg
     6481 ttagccagcc atgtatcgac catagacatc tcatcaacaa tcaaaagtcc gccttctaat
     6541 tctttcgtac tgactgaagg tgttttttcc cgtccattca gccccagaag acgatggatc
     6601 gtactggcag gcagacctgt cgtttcgttc attcgtttag ctgctcggcc agttggtgct
     6661 gcaagtagaa taggaaaaat ttcttgggta tattttgaag gatccaattc cagtccgttc
     6721 aactctgcaa aaagattgac gattccgttg atcacagtag ttttccccgt acctggtcct
     6781 cctgttaaaa taaatagagg ggaacggatt gcttcagcga tagcttgttc ttgcgaaggt
     6841 ccatactgta tgccgaatat tttttcgata cgccgaatat tcttctgcac ttctttttca
     6901 ggataatgga tttctttttt acgactgaga aggcgctgga tcgaattacc gatcccccat
     6961 tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt cttgttgtat ctttccttct
     7021 tcaacaagtt ggataattac agtagcaacc tgatcaggct ggatttcaat tgggcgactt
     7081 gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg ccggtacgta tgtatctcct
     7141 gtttgaactg catgctgaaa aatctgatgc aagatagctg ctcgaattct tctcggtgaa
     7201 tctgctgcaa taccgatttg ttcggctaga ttatctgcac gtttgaaccc gattccttct
     7261 atgtcttcga ctagctggta cggattttct tcgatgatat ccaaagactc gttcttatag
     7321 gcctggtaga tactgaaagc taactgacta ccgaacccgt aacgattcag tcccatgatc
     7381 acttgatcca tcccgtggtt tagccgaatc gtctcagcaa gcatttctct ttttggcttc
     7441 gtcaaaccgg agatttgttt taataccgaa ggatcagcaa tgattttttc gatagcatct
     7501 tctcccagca attctacaat tttttctgct gttttcttcc cgattcctgg gaatttttcg
     7561 ctggaaagat acgctacgat cccatttgca gttgttggct tttcttgctc atatctttcg
     7621 agtttaagct gccttccgta tttaggatga tccactaatt ctccataaaa acgataagtc
     7681 tcattttctt gaatatcgcc gaaacttccg gtgactacaa tctctttttc acggtaatca
     7741 gctgtggttt cactgatgtg taccaaaagc actttgtaaa aattactagg attttgaaaa
     7801 aaaatcgcgg caactgtacc aacgaaataa ggcatttctt ctggctgcat cggaagccgc
     7861 ctccttcttc tatagtaaag gttctggtgc tgtttcagtt cctaaaaaat ctgtttggtt
     7921 ccatactttc agttcatatg gcaataatct gcaaggctct tcagtctcta aaatcgtcaa
     7981 actactattg aacaatccac ccatttcacg caactcactt agctcttttc ctgtcatcca
     8041 ttgaatggct gcagttaaag aagctccatg tcctacgaat aaaagtgggc catctttatg
     8101 ttgggcaacc gcatctgcta caacagtttc gatacgtgtg attgccgatt caatcgtttc
     8161 tccgtgaaaa ggagaaggat catacagatc taaatgatta cgcaaattcg gcagattatc
     8221 cggatatttc tggtacattt catcgatcga ctgaccttct aaagtaccca gccctagttc
     8281 tcttagctta tctgtataaa aaatctctac cggacgagca agctctttgt tgataccttc
     8341 tgctgtatca cgtgctcgct ttgctgtact ggaataaatt gctttaaaag gaacatattt
     8401 tacctttttc ccaaatgctt tgatttcttc gtaactttgt ggcaaaagcg gagaatcgcc
     8461 attgctccct tgaaaacgtc tttcctgatt ccattgtgtc ttcccatgtc gggtaaaata
     8521 tagttccata cgcagcaaac tcctgtcttt cttttatttt actctataat aaactgtcta
     8581 atccagcttg tcattttcat agtgatgcgt caattctaaa ttagggaatc cttgctggcg
     8641 aagtgcttca tacactatca ttgcagctgt attcgataaa ttcaatgagc ggacatgctc
     8701 atcattcata gggatgcgta aacacttttc ttcgttttca cgcataaatg tttctggcaa
     8761 accggttgtt tcttttccaa ataaaaagaa gtgctcttct ccatcagcaa aattttcgtc
     8821 actatatact ttatgggcaa attttgagat caaatgtagt ttacgattac ctaaaaatgt
     8881 taaaaaagct tctaaattac tgtggtaagt aatatttaca tcattccaat aatccaaccc
     8941 tgcccgtttt agatgtttgt catcagttga aaaacctagt ggctcgatta aatgaagcgg
     9001 agtattcgtg gctgcacacg tacgggcaat atttccagtg tttgcaggga ttaatggttc
     9061 aaataaaacg atatgattca tcaggatcat tcctctcata tttatgttcg tttctatttt
     9121 ctgacaattt gatttttgca taaaaaaaaa cgtatcaact cggtgtcagt cactgcgagt
     9181 cgttacgtta gtgaagctct tctcactaac ttttatcaga acaggaacct gataaaaacc
     9241 aatgaaaaaa caacttgttg ctaatataac accctcaact tttttttgca acctctactc
     9301 attaaaaact gctagaaaaa cgttttctct tagttccttt taataattct atgtcaattg
     9361 taatcttctt caaaggattc ttatataagt ctgctttgat tcgctcttct acctgccatt
     9421 ctaaaggtga catggccaaa agatcttttt gattttcctc tggtatctca aattcatagg
     9481 ttacacgctg acgttcagtt aatttcattg cttcagcaaa cttggctact acacgttcat
     9541 tggaataact ctgtttttca ggttgattcg gataaaaagc ctgacgtagt tcctttaaat
     9601 aattttcttg tggcaccacc ttcaacactt ctccatcttc tgagagtaca cgttggaatt
     9661 cttggtaatg agaaggcgaa aagatattta aaaccgcgtt gaatgtttga tcagcaaacg
     9721 gcaaattggt caaatcagct acacaccaaa atgcttcggc agattgttct gtagctgcgt
     9781 agactccttc ttttgaaata tcaaatccaa cagcattttg aatctttttc aattttgaaa
     9841 gttcattcaa gaagctacct tctccgcagc caacatctaa tagcgagact tcttcaggta
     9901 aatgctgcgc aatagtctca ataacgggct gatacatgcc agattggatc attctacgcc
     9961 ttggaacgaa cattccttga tcgtattctg ttttaaccgc atgggataaa aaataaagtg
    10021 ttcctttttt ggaaaggtca aaacggtgat tatttttgca tatcaagcca ttatcagact
    10081 tttttacttc ttcttggcaa accggacagc ggaacagctg atgattttct gccaaaaact
    10141 ggcgtcctcg gtcaattttt tttaacatag ttccccctca tttctgtttt atcttaccgt
    10201 tagatgctca ctttggcaac tgctaaaaat atgatacaat gcctagaaga aagtgaaaga
    10261 taaaggagaa gattagatga tcaaagaatt ttgcgctgaa aactacacat ctattcctct
    10321 agcaattgct agaggagccg atcggatcga attgtgtgac aacttagcgg tcggtggcac
    10381 aaccccgagt actggtgtga tcgaagaaac gttaagttat gctggggaaa aagatatccc
    10441 cgtaatgacg attattcgtc ctagaggggg cgacttcgtc tataacgata tcgaattgaa
    10501 aatcatgcat actgatttga ttgaagcaaa gaaattaggg acggatggcg tagtatttgg
    10561 ttgtcttact ccttcaggct ggctagacga agaggcctta gaactattga tcgaaacagc
    10621 agaagggttg cagatcacct tccatatggc ttttgacagt attcctgaag atcggcaatt
    10681 cgaagcaatc gattggctgg tagaacatgg tgttgaccgt atcttgactc atggcggtcc
    10741 tgctgacaaa actattgtag aaaatttccc acggcttaaa gaattgattg cctatacaga
    10801 tggccgctta atcattttgc ctggtggtgg tgtcactgca gagaatgcag cacatgtagc
    10861 taaagaattg aacgtaagcg aagtacacgg aaccaaaatc gtggatcttc agttataatc
    10921 ttataaacaa aaaaacattt gcaacaggca taatgcttat tgcaaatgtt tttgtttttt
    10981 tacataaaat agcccataaa taaagaagca atattgaaat aaatcagtac actcgtcaaa
    11041 tcgctcaaag tcgtgataaa gggaccgctt gctactgctg gatcaaatcc tagacgatcc
    11101 attagcatcg ggattaaact acctgcaaga ttcgccactg tgattgcaaa tagcatagcc
    11161 atccctatga caaaccccaa gataaaatta tgtttccaaa aacctacgat tagaaaaatc
    11221 gtgatccccg taacagctcc tgtgacaacg ccggtcaaga cttcagccaa aacaagtcga
    11281 acaaatccat gatctttctc atcagaaatc gcaagtcgac gaacagccac agctagcgac
    11341 tgggttccag cattaccggc agttcctgta atcagcgaaa tgaagactgc taaaatactc
    11401 gcttcactta ccaagtcttc atagtgactg attaaagaag cggtcgacat acccaaaaac
    11461 aacagagtga tcagccaagg caaacgtcga gacgctgcct ttactggatt ttcattaatt
    11521 tcttccacat tgaccccggc aagtcctgaa taatcgctag cagcttcatc atcaataacg
    11581 tcgatgatat catcgactgt gacgattcct aacaaatgat catcataatc ggtaacagga
    11641 agagctaaga agtcataatc tctaaatgtt tgtgccacat cttcttggtc gtcgcctaca
    11701 tgaactgaaa gcactcgttc gctcatcaga tcagaaatca ttgcgtcgtc atcattcacg
    11761 attaggtcgc gcagagaaat aacccctact agctgacctt cttgattaac gacatagatg
    11821 taatagattg tttcagctac atctgcttcg ttcttcagca cgtacatcgc agagcgaaca
    11881 gtttgattgg ctacgatcga aacaaattca gttgtcatga tcgcgccagc cgtttcatcc
    11941 tcgtaatgga gaagttcctt gatttcactg gcatcgtctg ccgacatcaa acttaaatat
    12001 ttagcaattt gctttttatc cagcgtatta agcaagtcaa cagcattatc tgtatacatc
    12061 tcagaaagca tatcagctgc atagctagga cgcatttcat ctaggtattc tttcatatgt
    12121 tcgttgtctt cttcaatcac atcgaacata tccgctaatt ctttaggcga cagataggaa
    12181 tagatcagct gcctgttctc ttctgtaaga gattggtaga attgcccctg ctcatagata
    12241 tgcatctcta aaaaactgtt tctgaatgcc tgaatattat tttcccttaa atcttgagtc
    12301 aactgggcaa atctttcctc taattcttga ttttcatcca aaccaatctc acctcgcctt
    12361 attcttcaaa ccgtctcaca actgcctcca tgtctgcggc taaaggctgc ttgaactcca
    12421 gcaactcttc tgtaaatgga tggtaaaaac gcagatcata acaatgaagg gcttgtcttt
    12481 ttattcccag atccattcgc ccgccataca tatcatcacc gagcagggca cagccaattg
    12541 cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg cagctggata tcaactaacg
    12601 cgatttctgt tagtcgcttc tctatccagt actcagtctg tgccgaacgt ccttcatcag
    12661 agaccattcg cttcaatagc gaagaataat ctcgggcgat cggcaaatcg atatgtccat
    12721 gtgccgataa atctgaaagc cttccactga ctaaggcctg atattttttc acaaacttct
    12781 tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc atgctttgca aaaagcatca
    12841 aaccagatgt atcccgatct agtcgcgtca ccacatggat tacttggttt tcgtaacctt
    12901 gttttttata ataagccttt acacgattag ccattgtccc atttggatgg tattgggcag
    12961 gaatagacga aacgcctgct ggtttattta ctaccagaac gtgttcatct tcatacacga
    13021 tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc tccttcagca ggaatcacaa
    13081 tggttacctt atcatcctta gctagagaaa acaagacatt ttctacttga tcattaactt
    13141 tgatttgacc gccttgaaat tttattttcg ctaacagacc ctttgagatc cctttttctt
    13201 ttaaaaaata ttttacttgc tgagaatgat ctttctcata atcccatgta aactgcatat
    13261 caatcctcac taataaaggc atctttcaca cgatgccaaa aatgcatatg tcgataagaa
    13321 gcaaaatgga tccgttcttc tgctattcga taataaattg agcggatatt tgcttttgcg
    13381 acattcaatt gatcaaccgt tacaagataa tcttctgtat tttccaattt tatttctagc
    13441 cattcatcat gggcaatcac gatcggagaa cccaaagtac gaaacactcg attattcagt
    13501 gaagcaatct ctgccaactg aaaggcattg atactaggat ggatcacagc accgccgaca
    13561 cttttattat atgctgtcga accagtaggc gtagaaatcg tcaatccatc tcctctaaaa
    13621 ctttcaaaca gctcgttttt gatgtataca tcggctacca tcgtacgatt ggcccgttta
    13681 atcgttgatt cattcagtgc taaaaaatgc tggtccggtg tttcatccaa ataactgatc
    13741 cgaacatcca gcaagggata actaacactt tgttcccggt tcgttcgtaa gctgtctact
    13801 agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc ctaaatgacc tgtatgcacg
    13861 cccaagaaac gcacatcatt cagacaatga ctgtagtgat ggaacgccga caataatgtg
    13921 ccgtctccgc cgactgagat gaccaattct ggctcatgat catctcgttg aatccctgct
    13981 tgttctaaaa gtgcgttcaa acgttctgtc acttctatag tctttgcttc tttattatga
    14041 acgatcgcta ctttcatccc ctgcctcctc tctttcgctt tctttcttca aaagcgttcg
    14101 atagtagaca tcatctgatt ttcctcttcc atgggagaat aaccgctgtg cttcttggat
    14161 ttcttcacgg atactggaca tctcttcatc caactgatag gcagcttctg ccgcacgctg
    14221 aagtcgttca ctcatttctt ctggaaaaac gccttgatat ttataattca gcgaatgctc
    14281 aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg atttccgcca aaattttctt
    14341 ctcaccgctg atcagctgta caggatactc gatcaccaga tgataagaac ggtagccact
    14401 tgctttttta tttgtgatgt aatctcgttc ttggagaact ttcatgtctt ttcttttacg
    14461 gatgatctca acaacttgat ggatatcttc tacaaactga cacatgatcc ttaacccggc
    14521 aatatcagac atttcttctt ctaaacgtcc cattggaata tgccgtaatt gtgattttgt
    14581 tacaatacta tcgactggct tgactctccc agtgacaaat tcaatcggga catgtcgatt
    14641 ttgttcacgg aactgtttgc ggatcccccg cagtttcact ttcaattctg atacagtttg
    14701 ttcatatggt gctaaaaatg cttcccaatc tcgttccata gctgatacct ctacttcatt
    14761 aacattcatt gtttattcta ccatatatgc aaagtttttt cgtactcttg atttatgcat
    14821 tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga gtcgatcaaa tgacacaaat
    14881 gcttgaaatc gaatataaat ctatgctgac aaagcaagag tacgagatat tcatcgctca
    14941 ctatcaatta acagataaag atttccgtgt acagactaat atttatttcg ataccgttga
    15001 tgaacagctg aagaaacaaa attgtggcct acggatccgt ttcgttggtc atcaagctga
    15061 atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa acaacggata cctttataac
    15121 agaagaagca gaagcattta tcttcgaaaa acgtctgcct cgttcaggcg cagtttttca
    15181 aaaactttct gatttgaata tcgatcctgc ttcattaatc caaaccggaa agcttacaac
    15241 aaaacgggct gaattcccta ttgaagaagg acttttagct attgatgaaa gctggggaga
    15301 caatctacac gattttgaac tggaactaga ggtaggggat gcatctgtgg gaaaaattgc
    15361 ttttataaat tttttaaaac gtttttcttt accttatcgt cctgctaaga acaagatcca
    15421 acgtatgctt gaagctaaaa attagaaacc tttcattttc acaaggcact tgagttcatt
    15481 tttttacttt tttctgttaa gttataggat gtgaaagaat ctagtcacta gttgtataga
    15541 atctattttg ggggagattc atatgatcga aatctattta tttgttaatc ctttagggga
    15601 atactgtttc gaaatggaac aacagttatt acaatttatc gaagaagagt acgggacacc
    15661 atcaaaagaa aagatgcaat ttcgctttct tcctttggtc aatttgcaga caattgggga
    15721 tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag gaacgtaatc acttattctc
    15781 aatgacttat tctgcagcat tggactgcaa agcggcgcag tttcaaggaa agaaaaaagg
    15841 ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt aatgacatac catattctca
    15901 agaacttgct gaatccatat ttgaagaaat cggcggcgat ttgaatatgt ttaaagaaga
    15961 tcgtcagtct gacttcgtta aagaagtatt cttttctgat caaaaaattg ctcgggaaat
    16021 gggcatcaca aaacaccctt ctgcagtcgt ttacaattat gcctgcgacc gtgattttgg
    16081 tgttttagta gaagtaaaag gtacaagtag cctttggaga atcacagagc tgtgtaaaac
    16141 aacagataac tcatatcaag tttttaatgg tgaacattta catacaaatg attctcagtt
    16201 ccattcaaga actagccatt tacacttgct ttctaattaa aaaaatacgt cgactccctt
    16261 acaagagagc cgacgtattt ttttcgtttc tattcgagaa aatcaaaaat agaaagcctt
    16321 ataaacgatc gatcaattct tctaattcat ttagacgttg ttcaaacatc gacattgcat
    16381 cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt tttcattact tccacaggat
    16441 aatcgctgtt acctgctttt aggtaagcca agtagttctc tagtgcctct ggttcctggt
    16501 ttagtatttt cttcgccagt gctgaagcag cagaaaagcc agtcgagtat tggaaaacat
    16561 agtaattata ataaaaatgc ggtatccgtg accattcgaa tttaatttct gggtcttctt
    16621 cgactgctgg accataatat tttgcattca atttaccata actatcactt aggtattcac
    16681 tagtcaatgg cacacctttt tcatcttccg tatgcatgaa atgctcgaat tcagcgaatt
    16741 gtgtctggcg gaaaactgtt cctttaaacc catccaagta atggttgagt acataagccc
    16801 gtacacgagg gtctttttct gtttccaata aatactccgt taggatattt tcattcgttg
    16861 tcgaagcaat ttctgctaaa aagatggagt agtcgccgta cacataaggc tggttcgaac
    16921 gagtgaaata actatgaaca ctatgtccca tttcgtggac aagcgtaaat agctgatcca
    16981 gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc atagcttccc gaagaataag
    17041 caccgcttcg tttcccttta ttttcgacaa catcgatcca acgttcagag aatgcttttt
    17101 ctacgatggc catgtattct tcacccattg gtttcagtgc ttctaaagct ttttcttttg
    17161 cttcttcgta cgtaaaggta attggagctt cccctaagac cggtgtataa agatcataca
    17221 tgtgcagttt ttccacttct aataaacgtt tccgtaattc catgtatcga tgtaacaaag
    17281 gcaaatgctt gtttaccacg tctaccaaag tatcgtatac actttcagga atatgattat
    17341 tgctcaaaga cgcttctctg gcagagctgt aattacggac tttcgcttta aaattatgtc
    17401 cttttatatg tgtgcctaaa gtagaagcaa atgtatttct aaattgttcg taaacactgt
    17461 acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt gctttctagc aactgaccat
    17521 acacgccatg agataattgg actatttcac cattttctcc ttcaatcgtt ggaaaaacta
    17581 gatctgcatt attcaaaaca gcaaatgtat cacttgaagc atcaaagatt tcacctgctc
    17641 cagcaagaag agattcctgc tcagcagata agacatgggc tcgattatct actatttgct
    17701 ggatataatg gcgatagact tccaattttg gttcttcttt gaaatattgc caaatctggt
    17761 catctgacaa ttgcaatatt tctggttcaa accaggaaac ggcttcacta actttcgaaa
    17821 acagactgct tgctcttgca taaagcgctt ggtaatctgt atttccagtg tcttgatcgt
    17881 ttttaaggtg cgcatataca taaatgactt cagtttggcg ataaaccctc aatacgaatt
    17941 caatcgcatt taaaaattga gaagcgcctt gatcaagtgt tcctttgtgt ttttcagatt
    18001 gttttaactc ttctgataat tccaaatatt tctcatcaaa ctcttggtcg cttgaaaaga
    18061 tcttggtcaa gtcccaagtt aaattttcag gcaattcttc tcgttttggc aactgcttta
    18121 cttccatgaa atccactcct tttcttccct tagctctatc tatcaaaata gatcatagtc
    18181 taacatccac atgtcacagc atctaacttt tctactcatt catgtgtctg cttccatcaa
    18241 ttttagcata actaaatctt gttttacata gaaaaccgat tcctagccta tttttatact
    18301 tactattttt tcaaagaaga caagtgtatg cattcgttaa aaaactgctg atagaccatc
    18361 tcttcatcaa tcataggaaa aagccattcc cttttatgtt cttttatttg gcaaagccat
    18421 gagttgaaat caggaacttt ctggttttcc aaggcttctt cgtacaacat tcgataaaga
    18481 aaaacctgtt cttgtaaata aaagaaaaac cgactttttt gatagatcca cgatgataga
    18541 tgcaaaagat gtttatgacg caagtagcat tgttcttgta tggatactgt attcttttgc
    18601 ttgtgaataa gttggcgtgt cagccatttc ttcccatctt cactcacttt ttctattttt
    18661 ttcatttggt tcattttaat ttcgttacta gtgaatattt ctttaaggtc cctactagaa
    18721 aaagtccagc agctactgca aaaggaaata tgccctgata cagtctctgt aatatgatga
    18781 tacaaataaa gcttttgttt cttgagatca gctttccagc aatgcactcc tctttttcta
    18841 ttatagtagc agcaactttt ttctatcaat gagaattgcg attgtccaag aaaagaatta
    18901 ccaagtatcc accatacttg gtaactgtta ttcaaataat tttgggttct ttcaataaat
    18961 ctctggtgag acagtctaga acattggatt tctattgcta gattcgccaa caaaatatcc
    19021 ggcctttgcc gcaacctcgg caaataggct tccaaaaata ctggttctac tgactgtccc
    19081 agccaatcca tgaagcattc ctttaactgt aagtgctctt ttgattctct ttcactaaag
    19141 accgaacagt tatctgattt ttgatgggca aaatgagcac attttttctt tccctttttc
    19201 aaaatcactc tttttcgaca gccacgacaa taatatttct ttcctttttc tgcatgttta
    19261 gcaaaacagc acttattata ttcattcatt gcaagcagca tttttctccc tctgaactta
    19321 tttacgtcta agaatttatt tacacgaaaa aagagggcgt gaaaaagcta ttctgcatca
    19381 agtaataaga acagctaaac aaaaatagtt cccaatattt ttcgtttggt tgggcttgtt
    19441 accgcagatg caagcttttg aacacattta tacgaaaaaa agactgcgac acaatttttg
    19501 tcacagtctc ttactataat gctgatttat acaaaataaa atttcgttcg ctcgatagca
    19561 ttacgcgcca tcagacattg tccatattca ttcaatgttt ctggtgtacg atcgctcttt
    19621 tcagcaaact ccaaaattcg agcaatctga ttctctacat ccgcttggtt gagattttcc
    19681 atccagaagt gaacgactag gtaatagcgc tcttcgaata aatacaaatc agtccaagca
    19741 gactctagtt cgacatctgt tgcaagttgg atcacttgtt caaaatcttc aaaagcaaaa
    19801 gttgcttgtc tttcctgttc ttctagggcg cttccagata attcttcctc cggatcgctg
    19861 ccaataatct gctgacgaag aaagtcactc acatcttctg aatcaacagt agaatcttct
    19921 gattgagaaa tttcttcatc cggaagattt ttactgataa acagttctaa tccgtctcct
    19981 tttggcaaaa cttggaaagt gacagcttcg cttcctcgga actcctcttc gatatcaact
    20041 tcttccaaaa tactataaaa gaaattttcg atttctttgt gattgccaag taaatccaaa
    20101 aatgaaacgc cccgttcctc taaatcttca tgaccaatta gtacacggat cgtattttca
    20161 ttaatatgtt ccatttccat tttgctacac ctcactttgc cacaatgttt aatatagatt
    20221 cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc tcgctgttaa gcgaatttaa
    20281 taaaaccagc ggatttttca acctttcaag ctatcttatt taactataac atttttggca
    20341 gacgaatact atatagccga cttttcagct aattttccct ctctattttt cgtgcaatca
    20401 tcatcttcta ctaccttttt atacatctaa tcaagaatac tttttgtaaa aacaaaaaaa
    20461 cttggaaaaa ctatctttgt aatagtcttt
//
LOCUS       DACDSY010000037        20336 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDSY010000037 DACDSY010000000
VERSION     DACDSY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.037"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(13..570)
                     /gene="frr"
                     /locus_tag="IWK44_001143"
     CDS             complement(13..570)
                     /gene="frr"
                     /locus_tag="IWK44_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP9549680.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(573..1295)
                     /locus_tag="IWK44_001144"
     CDS             complement(573..1295)
                     /locus_tag="IWK44_001144"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP9549681.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1431..2312)
                     /locus_tag="IWK44_001145"
     CDS             complement(1431..2312)
                     /locus_tag="IWK44_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP9549682.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2411..3193)
                     /gene="rpsB"
                     /locus_tag="IWK44_001146"
     CDS             complement(2411..3193)
                     /gene="rpsB"
                     /locus_tag="IWK44_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP9549683.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3552..4031
                     /locus_tag="IWK44_001147"
     CDS             3552..4031
                     /locus_tag="IWK44_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP9549684.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4248..5339
                     /gene="phnW"
                     /locus_tag="IWK44_001148"
     CDS             4248..5339
                     /gene="phnW"
                     /locus_tag="IWK44_001148"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP9549685.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5332..5460
                     /locus_tag="IWK44_001149"
     CDS             5332..5460
                     /locus_tag="IWK44_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9549686.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5464..6009
                     /locus_tag="IWK44_001150"
     CDS             5464..6009
                     /locus_tag="IWK44_001150"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9549687.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6463..8154)
                     /locus_tag="IWK44_001151"
     CDS             complement(6463..8154)
                     /locus_tag="IWK44_001151"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP9549688.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8574..9521)
                     /gene="arcC"
                     /locus_tag="IWK44_001152"
     CDS             complement(8574..9521)
                     /gene="arcC"
                     /locus_tag="IWK44_001152"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP9549689.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9636..10655)
                     /gene="argF"
                     /locus_tag="IWK44_001153"
     CDS             complement(9636..10655)
                     /gene="argF"
                     /locus_tag="IWK44_001153"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP9549690.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10746..11975)
                     /gene="arcA"
                     /locus_tag="IWK44_001154"
     CDS             complement(10746..11975)
                     /gene="arcA"
                     /locus_tag="IWK44_001154"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP9549691.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12436..13137
                     /locus_tag="IWK44_001155"
     CDS             12436..13137
                     /locus_tag="IWK44_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9549692.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(13746..14762)
                     /gene="tsaD"
                     /locus_tag="IWK44_001156"
     CDS             complement(13746..14762)
                     /gene="tsaD"
                     /locus_tag="IWK44_001156"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP9549693.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(14759..15223)
                     /gene="rimI"
                     /locus_tag="IWK44_001157"
     CDS             complement(14759..15223)
                     /gene="rimI"
                     /locus_tag="IWK44_001157"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9549694.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(15230..15772)
                     /gene="rimI"
                     /locus_tag="IWK44_001158"
     CDS             complement(15230..15772)
                     /gene="rimI"
                     /locus_tag="IWK44_001158"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9549695.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(15756..16580)
                     /gene="tsaB"
                     /locus_tag="IWK44_001159"
     CDS             complement(15756..16580)
                     /gene="tsaB"
                     /locus_tag="IWK44_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP9549696.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(16669..17649)
                     /locus_tag="IWK44_001160"
     CDS             complement(16669..17649)
                     /locus_tag="IWK44_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9549697.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(17673..19157)
                     /gene="galT"
                     /locus_tag="IWK44_001161"
     CDS             complement(17673..19157)
                     /gene="galT"
                     /locus_tag="IWK44_001161"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP9549698.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(19169..20158)
                     /gene="galE"
                     /locus_tag="IWK44_001162"
     CDS             complement(19169..20158)
                     /gene="galE"
                     /locus_tag="IWK44_001162"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP9549699.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         5652 a         4247 c         3618 g         6819 t
ORIGIN      
        1 tttatgattt tattaaactt ccaataattc tttttcttta tcagcagcga tcttatctac
       61 ttctttgatg ctgttatctg tcaatgtttg tacgtctttt tctaatccgc gcaaatcatc
      121 ttcggtaata tcaccatttt tttgttgttt tttatattca tcgatcgcat cacgacggat
      181 gttacgaatc gcgatttttg cattttctgc ttctttttta acatctttag ctaattcttt
      241 tctgcgttct tctgtcagtt gcgggataac taagcgaatg acatttccat cattggttgg
      301 actgattccg atatcacttg ccataatagc tttttcaaca tcttgaagaa tgctcttgtc
      361 aaaaggtgtg atcattaaaa cacgtgcttc tggaatttgg attgatgcca tttggttcaa
      421 tggtgttggc gcaccgtagt agttcacagt aatacggtcc aaaaggctag cgttcgcacg
      481 gcctgcgcgg atttgcccaa gttcacgttg aagattttgt gctgcttttt tcattttttc
      541 ttttgcttct gtcataattg catctgccat ttttatttcc cccttacagt tgttccgata
      601 ttttcgccta agattgcacg gcgaatattt ccgtgttcat ttaaattgaa gacaagcagt
      661 ggaatatcat tatccatgct tagtgaactt gcagttgaat ccatgacttg taatcctttg
      721 gcaataactt ccagatgagt cagttcttca aattttactg catttgcatc tactttcgga
      781 tcagcagaat aaacaccgtc tacattgttt ttagccatca ggataacatc tgcgccgatt
      841 tctgccgcgc gtaatgctgc tgttgtgtct gtcgagaagt acgggttgcc ggtaccgcct
      901 gcaaagataa cgatccgtcc tttttctaaa tgacgttctg ctttacggcg aatatatggc
      961 tctgcgattt gacgcatttc aatcgaagtt tgcacgcgtg tcggtacacc cacgttttcc
     1021 aacgtatcct gtaaagctaa ggcattcata actgtagcta acatacccat gtaatcagct
     1081 tgagcacgtt ccatgcccat ttgtgctccg atttgtccgc gccagatatt tccgccgccc
     1141 acaacgattg ccatttcaac gcccagctcg tgaacttctt tgatttcttg gatgatttct
     1201 ttgattactg gtggtttgat tccaaatcct gcgtccccag ctaaagcttc tccactcaat
     1261 tttaagacta cacgctgata tttcggtgtg accatgttca ttcctccaat attttctatt
     1321 gccattttac catacttaga tgatttcgac actctaagac tggtttttct tgttttgctt
     1381 aaaaaaaggg agcgcacaaa ccgtgcgttc ccgtctgtta ttcaacagaa ttattttttc
     1441 acttggctca taacttcttc tacaaagtta tcttcgcgtt tttcgatacc ttcgccaact
     1501 tcgaaacgta cgaatgtttt aacagttgcg ccttttgatg caacgtattt ttcaacagtc
     1561 atatcaggat ctttaacgaa tggttggtcc accaaagcga tttctgcttt gaatttcttc
     1621 aagcgtcctt ctaccatttt ttcaacgata ttcgctggtt tgccttcgtt caatgcttgt
     1681 tctgtaagaa cagttttttc atgttctaat tcagcttctg gaatttgtga ttcgtttaca
     1741 taacgaggat tgatagctgc tacgtgcata gctacgtctc ttgcaactgt ttcgtcagtt
     1801 gttccatcta gaacagtcaa tacggcaata cgtccgccca tatgcaagta tccaccaaat
     1861 gcagcattgt cgtctttttc tacaacttca aagcgacgga agctgatttt ttctccgata
     1921 acttgagttg cttcgatcaa gtcagattca attgtacctt tatctgtttt gattttcatt
     1981 gcagcttcca tgtctgctgg tttgttttca gcaacaagtt ctgcaatttc ttttactaaa
     2041 tcttggaaca tttcgttttt agaaacaaag tctgtttctg agtttacttc aacgattgct
     2101 gcagtgttac ctttcacagc aactgaagcc aatccttcag ctgcaatgcg gtcatttttc
     2161 ttcgcagcct ttgccattcc tttttcacgt aaaagatcaa ctgctttttc catgtcgcct
     2221 tctacttcaa ctaatgcttt tttcgcatcc atcattccta cgccagtcat ttcgcgtagt
     2281 tcttttacca ttttagctgt aacgtctgcc atgtttaact cctcctgtgt gtttttcttt
     2341 ttaaaaaaag ctgcttcaaa ggagagtgag gcttgtcgcc cagcctttga aacagctcca
     2401 tcattcaaaa ttattctgct gaagaattgt cgccttcaac aacatctacg atttcttcga
     2461 ttgatgttgc tgtgttttct tctgtgaaat cttcttcaac tacttgatct tcaccttggt
     2521 ttccttcgat gaaagcatca gccatttttg aagtaatcaa tttaacggca cgaattgcat
     2581 cgtcgtttga agggattact acgtcgattt catctggatc gcagtttgta tcaaccatag
     2641 ctacgattgg gatatttaat ttgtgtgctt cttgaacagc aatacgttct ttacgtgggt
     2701 caacgatgta cattacatct gggattcttg gcatatcagc gataccgcct aagaattttt
     2761 caagacgttc acgttctttg ttcaaaccag caacttcttt tttaggtaga acttcaaaag
     2821 ttccatcttc ttccattgca ttgatttgtt tcaaacggct gatacgtttt tggatagtat
     2881 cccagtttgt caatgttccg cctaaccaac gatggttaac gaagtattgt cctgcacgag
     2941 ttgcttcttc tttgattgct tcttgtgctt gtttttttgt acctacgaat aatgctacgc
     3001 cgccttcttc agcaacgctc ttcatgtaat cataagctgc atctactaat ttaactgttt
     3061 tttgcaagtc aatgatgtag attccgtttc tttctgtgaa gatatatttc ttcatttttg
     3121 gattccagcg acgagtttgg tgaccaaagt gtacgccggc ttctagtaat tgtttcatag
     3181 aaattactgc catgtttgtt tcctccgatt tggttttatt tttcctcttc ttatcttcta
     3241 ccttgccggc aaactttttc aagcacctac cgacaatcca ataagaatgt ggatttgctg
     3301 atgcataaag caccctcagc tattatacag acaaaagatc cgtatcgcaa gagcaaatat
     3361 acttttctgc tcaacattca atagtttacc tgaaagataa aaagaattca agaaaaacag
     3421 tagatttctc tttgtttctt tattatcctt tttccataaa ataattttag gtataaaaga
     3481 cgaatattat tgggcaatcc ttcttttttt tgttatgata catgtgaaat ctttcgtaaa
     3541 ggagaaagaa aatgcgcaaa gctcaaagac atttgctgat taaacaagtc attgaagaat
     3601 tcccgatccg tacgcaagaa gaactattaa acaaacttgg gcattatggc gttaccgcca
     3661 cgcaggcaac gatttctcga gatatccgtg atctaaaaat cgtgaaggca cctgatgaaa
     3721 atggcgtttc ccgcttcgta ttgtttcgcg aaaaaggaac tcctgagact aggactgagg
     3781 aagaggatcg gctggttcaa atgattgaag acattgtatt gaaagtcgaa cgagtccact
     3841 ttttgacgat cgtcaatacc ttgccagaca atgctcattt atttgcatct gttttagatg
     3901 agatcaaacc tccctctatt gtcagtacaa ttgctggatt cgatacgatc atggtcatct
     3961 cggaaaatga ggaggacgca aaacgagtag aagaatttct tcaccatcca actgaagtat
     4021 cactttttta atcagagcac gtattcctaa tatggaatgc gtgttttttt attttcaata
     4081 ttttccttat tgaataaacc tttacactct ttacaaaaat acatcatttt ttattcaaat
     4141 ttttcaacac cttgattgaa tcacaataat catcatttat attggatgtt aacaagagca
     4201 gcttcttgct ctttgtgtat ttcaaaaaag aaatgaaagg aaatcatatg tataaattat
     4261 taacttctgg accattgacg acttctgaaa cagtgaaaaa tgctatgctg atcgatcatt
     4321 gtacttggga tcaagaatac aaagaaatta cacaatggat aagaaaaaaa ttattggatc
     4381 ttgctcaagt atcggaagga atatacacaa cggtcttaat gcaaggtagc agctccttcg
     4441 gggtagaatc ggtattaagt tctgtgatca ggccagaaga tactgtactg atttgttcaa
     4501 acggcgcata tagacaacga atgatcaaca tgtgcgaatg tctgaaactg aactataccg
     4561 tttatgaggt atcggaacat caaatcctcc aagctcatgt gattacacag ctgattgaag
     4621 agaatccgtc tatctccgca ctagcaatgg tccatagcga gactacttcc ggtattttga
     4681 ataatttgga agagattgct gctgtcacac aagaaagaga tcttttgttt atagtggacg
     4741 cgatgtcaag ctttggtggg ataccgattt gtgtagccga actagggatt gattttttga
     4801 tttccagctc aaacaaatgt attcaaggtg ttccaggctt ttcttttgta cttgctaata
     4861 aagcggaact ggaaaaacga aaaaatgtta gccgaagttt gtcacttgat ctatatgacc
     4921 agtaccgcgt gatggaaaac gagaatggca aatggcgttt cacttcccct acccatacgg
     4981 ttttggcttt tcaacaagcg ctgaaggaac tagaggcaga aggaggaatc gaaactcgat
     5041 attcacgtta tctcgccaat aatcatgtgc tacgacaaaa aatgacagag atggggttcc
     5101 agcctttcat tcaagaatat caggggccat tcatcacctc ttttctttac cctaaggact
     5161 ggcgatttga ttttaatcat ttttatcagt atttaaagca aaatggattt gtgatctatc
     5221 ccgggaaaat cagcgatcat gaatgttttc gtatcggaaa tattggcgag atctatccgg
     5281 aagatatttt ggaactgaca gcaattatca aagactataa ggagaacgtt aatgattaaa
     5341 actgtaattt ttgattgggc aggaacaact gtggactttg gctgtatggc tcctgtccac
     5401 gcttttagaa atgcttttct agaaaaagga atccaactga ctgacaaaga aatccgctaa
     5461 ccgatgggaa aattaaaatg ggatcatatc caacagcttc tttccctccc ttctgtcaaa
     5521 aaacaatgga tagctattta tggacaacta cctcaggaaa aagaggtgga ggaattgtat
     5581 caacaatttg aacattatct gtttaaagat ttggtcaagc attctacctt gaaacccgac
     5641 acattggaaa cgatcgaacg gttgagaaac gctgggatca atattggttc cacgaatgga
     5701 tttaatgctc ctatgatgac aatcgtagca gaaaccgcaa aaaaagaaag gtattcacca
     5761 gacttcatgg caacttttga agacgtcgaa gggtatggtc gaccgtttcc ttatatgatc
     5821 cataaaaaca tgcagtactt caaaaacaag tcggttgatg aagtaatcaa agtcggagat
     5881 acggtctcag atatccagga agggaaaaat gccggagtgt tgacagtagg tgtaattgaa
     5941 ggtagttcgc taatggggat ttcttatgat gagtaccaaa aattagagat ggggaaaaag
     6001 atcgtttaaa acagtctgtt gctcttagat tccaacaagc cggcgctgat atttgtgtaa
     6061 acaatttaaa agagttaaca gaatacattt tgtacgctga catgaatcca gaatgacttt
     6121 tatacaaaat aagaggttgt gaaaaagctg tcctgtatca agtaataaaa acagccaagc
     6181 aaaaatagct cctcatattt ttcgtttggt taggcttatt accgcagatg caagcttttg
     6241 aacaccgttt attcgaaaaa aaaaagggag ctgtgacacg acttttgtca cagtcccctt
     6301 atttgcaaac aaacagtgtt taagagacaa cctctccact aaacggtgtt cattcagctg
     6361 actctcggta tttgctcaaa aaacgataaa atttgaaaag ctattttctc ttttgtttct
     6421 ctaatactcg gatcagaacg ctgatttcac attctctttt tcttacatct tatctggtgc
     6481 atgaagtcct agcaaacgaa ggtcttcttt caataataca gtcactgcat aaactagagc
     6541 taagcgtgct tctttttgtt catcatcagc taaaatcttc gtatgagcat agtatttgtt
     6601 gaatgcttgt gctaatttga ttgcatgttt cgcaatcact gacggttcgt atttttcccc
     6661 agcagacaag accgtttcag ggtatttttg aactaattta accacttccc agctggtatc
     6721 gtcattcaat gcatagtctg ctgtttctga agggacaaat cctgcttttt ccaacaggct
     6781 gactgcacga gcatgtgtat attgcacata aggtccagtt tctccttcaa atcgaacaac
     6841 ttcttccagg ttaaagtcaa atgtattcag tcgatcgttt ttcaaatcgt ggaaaatgac
     6901 tgccccaacc ccaacttgtt tagctacagc atctttgttt tccaaatcag ggtttttctc
     6961 gctgatttgt tctttagcag attggattgc ttcatttaat acttcttcta ataagacgat
     7021 ctttccttta cgagttgata attttttacc accttgcgtg atcaaaccaa atggaatatg
     7081 gtgcatgtcg tcagaccagt caaagcccat ttccttcaag acagctttca actgtttaaa
     7141 atgatagctt tgttcattcc ctactacata tagagattgt ttgaagtcgt aagtacgttt
     7201 acggtacaat gcagcagcta agtcacgtgt aatgtatagt gtcgcaccgt ctgacttctt
     7261 aatcaacgct ggattcaggt cataagcaga taggtcaacg atctctgcac ctttatcctc
     7321 gttcagcaaa tgtttttctt ctaaaagttt gacgatttca tccatcttgt cattgtagaa
     7381 agcttcaccg ttcaacgaat cgaaacgtac ttccagcaga tcatagattt tattgaattc
     7441 tttcatcgat tcatcacgga accattgcca taattgaata gcctctttat cgccttcttc
     7501 cagacgtttg aaccaagcac gagcttcttc atttaattct ggttctgttt cagctacttc
     7561 atggaattgg acgtataaac gaagcaattc attgattggt tctgctttaa cagcttcttc
     7621 tgtaccccat tttttgtaag cgacgatcag ttttccaaac tgtgttcccc agtcacctaa
     7681 atggttgatg cggattggct gataaccgat tttttccata atgaaaccga tcgaattacc
     7741 gataactgtc gaacgcaaat gtcccattga gattggtttg gcaatattag gagaagacat
     7801 gtcgattggt actgttcctt gattcccgat gttgctatcc ccataatgtt ctttttcttt
     7861 cactactgtt tgcaaaactt ttttgctgat aagttcttta ttcatgaaaa agttcaagta
     7921 aggtccgaca acttcgattt tttcaaagtt ggcagaatcg attttttctg ctaaatctgc
     7981 agcgatttgc tgtggcgctt ttcggtaaac ttttgctaat gaaaaggctg gaaatgcaac
     8041 atctccgtgt tcagctgatt ttggattttc tagtaattgc tcgacttgtt ccagtgttaa
     8101 atcatctttg acagcgtcat agacagcttt tgcgacaaga tctttattat tcatctttat
     8161 tcctcctaaa gtgttccctt gattgttaaa caaaaaaaat ctctagcgac ggcatactta
     8221 gtatgccatc actagagacg agatttcccg cggtaccact ccaattgtcc taaaaaagga
     8281 ccagctccat ctgatatcgg tagacgaccg tctgtttaaa acagaattct ccaagtgcgc
     8341 ttcgttgtat cttcttcatt cggcttccac cacttccgaa ttcgctagcc aagaatgata
     8401 caagtactct cttgttcaac aaatcttagt cttattaaac tttcttgaat tatatcagaa
     8461 aaaaaaacga aaggctagtc gtttcataaa aaaacaatcc agatggccta aaggagaaga
     8521 aataccacct ggatagctat aaatcatttc gtaaatacga aagacttaac aaattaatca
     8581 gctacgataa tcgttccttc ttcagaagct aaaagattct cgatattttc taaagaagtg
     8641 atgattgcct tagcgtttgg ttttgcttca acaaatgcaa tagctgcttc tactttcgga
     8701 agcatgcttc ctggagcgaa ttgcttttca tcaatgtatt gtttcatttc tgaaacagta
     8761 acagtttcta atttcttttg atctggtttt tggtaattga catacacatt gtctactcct
     8821 gtcaatacga tcagcaaatc cgcatcgatg atttcggcta atttttctga agcaaaatct
     8881 ttatcgatta cggcttctcg tccttctaat ccagtagctg tttctacaac aggaatccca
     8941 ccgccaccaa cagagactgt gataactcct tgatcgacca gcgtttcgat gacacgtgct
     9001 tctttaatcg agatgggttt aggggaagcg actacttttc tccatccacg tccagcatct
     9061 tctttgaatg tcacagtgga gtcagcattc atttgttctt tggcttcttc ttccgtgtag
     9121 aatggaccga ctggtttact tggatttttg aatgaaggat cattttcatc cacaataact
     9181 tgagtgacta aagaaaccac atctttatcg atcccttttt ccttcagtac ttcgcccatg
     9241 gcgttttgta accagtaacc gatagatcct tctgtcattg caacacatgt atctaaaggc
     9301 attgccggtg ttttctcaga atcagcagct tgctgctgaa tcaataggtt accgacttgc
     9361 ggaccatttc cgtgggagat gatcaattca tctccttgtt cgataaattt cactaaatac
     9421 tttgctgttt ccattaacgc ttcttgttgg gcttttgcac ttgcatccgt agacaagatg
     9481 gcattgcccc ctaaagcgac aactactttt cgtttggcca tttcattcgt cctttcttca
     9541 gttctaccga attttctaga taacaaaacg agccagcgtt ttgtgatttt ccctcaatgc
     9601 tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa cgcgagggat aaacaagtta
     9661 cctaatgttg ctgccatgat cgctttgatt gagtgcatac gattttctgc ttggtcaaat
     9721 tgacgagcat atttgctgcg gaagacttca tctgttactt ccatttcagt gatgccaaag
     9781 cgttctttca tttgttcacc gtaaacagtg tttgtatcat ggaaagctgg caagcagtgc
     9841 aagaagatca aacgatctgt gttgtgtgtt ttttctacca tttccatatt gatttggtaa
     9901 ggtttcaaca atttgatacg ttcttcgaat ttatcttctt cacccattga tacccataca
     9961 tcagaataaa ggacgtctgc tccgtctaca cctttatcaa catcgtcggt aatcatcaat
    10021 tgtgcaccgg atttttcagc aaagccttct gccattttga cgatttcttc ttcaggttgt
    10081 aattcttttg gtgcaacgat acgcatattc gctcctaaaa ttgcacctgt gactaataaa
    10141 gagttagcca tattgtttcg accatctccg cagtaagcaa cagtgatacc ttctactgtt
    10201 ccaaagtttt cttggatagt taaaaagtca gcgatcattt gtgttggatg ccattcatca
    10261 gtcaagccat tccaaactgg aacaccagag aattctgcta gttcctctac cattttttga
    10321 ctgaatccac ggaattcgat tccgtcaaac atacgtccta gtactttagc tgtatcttct
    10381 gtggattctt ttttacctaa ttggatatca tttgcaccta gatattcagg atgtgcacct
    10441 agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg tagatgtttt ttcaaataaa
    10501 agcgcaatat tttttccttc tagatagtga tgtgggatac cacgtttttt caagtctttc
    10561 aagtgttctg aaaaatcgat caaatattgt aattcttctt ttgtaaagtc tttttctgct
    10621 aataagcttc ttccttggaa tactgattct ttcatattaa acattctcct ttagattgtt
    10681 tttatttaag attaaagagg aaatcccgac ggcagctgac agtaagactg ccgtcaggaa
    10741 acaaattact tcaagtcttc acggacaagt ggttggctca tgcagcgtgg accaccacga
    10801 cctcgtgaca attcacttga attgatttct aacactttga tgccatagct acgaagcaat
    10861 tcgttcgata cataatttcg gttataagtg actacaacac ctggagcgat tgccaatgtg
    10921 tttgatccat cattccattg ttctcgaggt gcaacgatct catctccatt accagttggg
    10981 atcagtacaa gatcatctaa tccaagagca gctttcatcg tttcgtgtaa gttatctgaa
    11041 tgagtaatct tgatatcatc accatctggt tcgattgtaa agacatccat cttaccgtct
    11101 ttactttgaa ttgctggatg gatagtgaat ttatcgtgat ctaccattgt aaatacggta
    11161 tccaaatgca tcattgcacg gttgttcgga attttgattg ccaacacttt ttcaaatcct
    11221 gaattttttg cgaataggtg gcgtgctaat ttttctaaag ctttcgcatt agttcgttga
    11281 gaaatcccaa cagctactac tttatcgctt aagatcaatt cgtctccgcc ttcgatatga
    11341 tctggatttt cacgatccaa ccatacttcg attcctttat ttgcaaatct tggatgatgt
    11401 ttcaaaatat attctgtaaa catcgattca cgacgacgtg cttcaaaagt catcttattg
    11461 actgtcatcc catttcccat tgatgcggaa gggtcgcgtg taaagtacaa gtttggcatt
    11521 gggtccatgt aaaatggata gtcatcgtct gctgacagat catatagact gctagaacgt
    11581 acttcgatat cttttgtacg aaccccagcc atgattttat ctaccatttc ttgcgtttcc
    11641 attgatagaa ggaactcatg aagaccctct cttacagcat tcgatgcaat atgagattca
    11701 tccaacattt tattcaagaa ttgttctttg acattgcctg cgtcaatcgc ttctgccgct
    11761 aatttttcca agtaaagagt ctcgacgcct tcattttgca atgtcttcgc aaagttgtca
    11821 tgttcttctt gagcaatcgg aagatatgga atatcatcga acaacagacg atccataata
    11881 tctggtgtca aattttccac ttcttgtcca ggccgtttca gtaaaactgt ctttaatttc
    11941 cctatttcag agaaaacgtg aataggttta tccatgttgt gttgcctcct tctttttgat
    12001 tacatgtata ggatatcctc cttttgtaat cactttcata attttgtctc attcaaaatg
    12061 agacattcct cacattaata ttcgataatt aaataattaa acgtttttct atttttaagc
    12121 gtaatttatt cattttttat tctgtataca ctttcaaaaa aatatttatt cattgaaaaa
    12181 atttttttga ataaattaaa aaaataaatt atattatcgt tgttagcgtt tactatatgt
    12241 attattgttg ttttttatac acaaatcatt cgcttcaaaa caaattattc aataccattg
    12301 tatatttttt acataataac aaattatatt tgtaaagtat taaaacttgt ttctaatgtt
    12361 ggtatgttaa aactttacaa attctttaaa aaaagtgaca ataaatatag tgaacaataa
    12421 ggagaggact ttttaatgcg tgttaataaa atcgattttc acttttcaaa actcagatat
    12481 caacctgatt ttgaacattt ctcggatgaa gagttccaac agatcaagga acatactgtt
    12541 cttcgttctt ataaaaaagg acagatcctt ttcgacgatg gtgatcccag acaacggttg
    12601 tatatcttga ccaaaggact cgttcgttta gaacgttatg atgaaagtgc aacttattac
    12661 tactatgact atgtgaatac aaataacctt tttcctatgt caggaatgtt tggagaagag
    12721 acctacactt atacagctca agcgatgact gatattgaaa cattctacct tcctgtttcc
    12781 tactatgaaa aattagcacg aaaaaatccc gaacagttga tctatatgat acaaaaaatt
    12841 tctaaagtag ttgaaataca tgaattgcgt atccaaatcg gattgacctc tagcgccttt
    12901 gaccgggtaa aacaaaattt attcattttg aaagaagaat tgggcgtacc aacaaaagat
    12961 ggtcatatca ccattcctta tccgatcacc ttaaaagaat tagcagtgaa tagcggcacg
    13021 acacgtgaaa ctgctggaca agtaatcaaa caactacgtg attccgggct gttagattat
    13081 gacaaaaagc gttttacttt ctgtaagaca gctactactc cagctatgag agaataacgc
    13141 gaacaaaaat aagcagtgac cctgataaat atcagggtca ctgcttattt tcctccctaa
    13201 aacttctgaa agatccggca gttttagatc actggacaag actcaaagat tttctatgtg
    13261 tgtttcagtt atgtaatatt cactgtttgc aaaaaatact gttagattaa ctagatgaag
    13321 cgtcttgtcc tcatccgatc tagattattc gatattcctc gacaataaac gattactcag
    13381 tgagggcaat cagaggaacg gataatgata aataagctgt aaaaaatagc aaaataacaa
    13441 taatgattaa aagtatgtaa aaaatagggc tttgacaaaa acgtgtcaca gccctatttc
    13501 gattaaacgg tgtgtacctg tcaactcttc ggtcacaagt cataatgttc ataaacatga
    13561 ggagctgttt tttgaaattg ttccttgtga atcagagtta aacgacaggc ttacgaactc
    13621 ttgattaaac ggcgttcaaa agcttgcata cgcggtaata agaacagcca ggtcaaaaac
    13681 atgagaagcc gtttttcctg ggctgttctt attacttgat gcagggcagc tttttcacaa
    13741 cctctttatt cagataaaac caagcttgga tcagcattca actgatagct agcaaaattt
    13801 cttttttgca tttccacatg tgctgctgct ccaatcattg cagcattatc tccacaaagt
    13861 cgaagagggg gaatcactag ttctacctca gggagtttag ctgccaaagc atcctgcaat
    13921 ccttcacgca acccttgatt ggccgctaca cctccagcta taatcagttg cttgactgga
    13981 taattttgac aagctcgaag tgttttagta atcaatacat cgatcacact tgcttgaaag
    14041 ctagctgcta aatcattttt gtctagatct tctccacgct gttgtgcatt atgcaccaaa
    14101 ttgataaatg cactttttaa tccgctaaaa ctgaaatcat aattatcttc atggatcatc
    14161 gctcgcggaa aatggtaatt atcttttccc tgatgagcta gctgatcgat ttcttttccg
    14221 cttgggtaag aaagacctaa tactcttcca actttgtcat aagcttctcc tgctgcgtca
    14281 tctcttgtct ccccaatgat ttcatatgat ccatcttctt gcatatagac taattctgta
    14341 tgtcctccgc taacaagaag tgccatcaat ggaaactgga atggtttaac cagacgggct
    14401 gcatagatat gccctgccat atggtttaca ggaataagcg gcaattggtg tgcccaagca
    14461 aaagccttag ctgcagaaat accaatcagc aacgagccga caagtcctgg tccataagtg
    14521 acagcgactg ccgataaatc ttctgccgac acaccagctt ctaccagtgc atcttccaag
    14581 cataaggtga tttgttctac gtgatggcgg ctagctactt caggtactac gccaccaaaa
    14641 cgtttatgac tgtttatttg agaagcaacg atgttagata agatctctgt tccatttcta
    14701 acgaccgcta cactcgtttc atcacaactt gattcaatcg ccagaatcaa ttcttcattc
    14761 atttcatcgt ctccgttttt aactttctgc tcataatgat cgcatcttcc gtcggattgc
    14821 gataatatga tttccgttta cctaatatac gaaacttttc tgattcataa agttttcttg
    14881 ctgcttcatt agatagacgt acttctaaaa aaacatgcag cgaatctttt atttgttccg
    14941 taatcagtaa ttgtaacaaa tgccgtgcat gcccttttcc ttgttctgaa actgcaacag
    15001 caatatttgt gatctcgact tcatctatta ctttcgagta tccaataaag ccacagatct
    15061 ggttatcttc agctaaaagc agataatcag agtgaggctg ttggatgtct gccaaaaact
    15121 gcattttcgt ccaaggtgaa ccatgtggat aagcattttc gctaatcatc catacgcatt
    15181 ctgccaagta cgcttcatca aaatcttttt ttctgcaaat caactaatct cactcatttt
    15241 ctaaaagttt tatcatatcc aacgcatctt cttttgtttc agtgtaatat ctttttttga
    15301 tcgttgttgc ttgaaacccc aatttccgat aaagtctttg tgcatcttga ttactcatac
    15361 gcacttctaa tgacatcgtc tcacagcgat tcattatagc aaagttctcg atttcgtcaa
    15421 taagcagcga accgattcgt ttacgctgat atgccggtcg aacggcaata ttcgtgatat
    15481 ggcaatctaa accgaacgct cttgaaccga taaaaccaat cattcttcca ttatcaaaga
    15541 tacctagata tagatgagga agcggtgact tcaattctga tataaaggca ctttttgtcc
    15601 atggcaattc gccattatag acttctcgtt cgacatccag cagctctttg atatcttctg
    15661 tagtcaattc tcttagtacg taacgacgat cgatttgttc gactgttttt tctgtataaa
    15721 tcattttatc ttgaaaaaag cttctggcat acgctctaaa ttttttcaac ataactttcg
    15781 tctccaggtg tgtgcgtctc caaccatttt tcttctgctt ctactcgttt taaatagcga
    15841 ggaagaaaag cttgtttctc tgcaactggt tcaactaaag cacctaattg agccacagtt
    15901 acaccacttg gaatatccca tgctggaact gtattaatca tcgcactagg caatatttcc
    15961 tttatttcag agaagaattt gtgacaatca ctcccgacga aataaatagc ttcattcagc
    16021 ttttgtagtt gtgtgaacaa atcagaagca gaaatatgta catcttcaag tacagtttct
    16081 aatctatttt ctttccagcg gtatgcccca gcgtatacat tttttcttcg agcatcaaac
    16141 atgggcacaa taatattatc cacaccgatg caattagccg cgatggtttt cagactagat
    16201 actcctacta gctctttatc taatgtataa gcaatcgttt ttgctgtcgt tacacctata
    16261 cgaagccctg tataagaacc gggaccatca gaaaccgcga ttcggtcaat gtcttttgga
    16321 cttatttgaa gatctgcaaa caattggtcg attgcaggca tcagtgttac actgtggttg
    16381 cgtttgatat ttgtttgtat ttgtccaaga atttgttcgt cttctaccac accgatcgcc
    16441 aaagtttggt ttgcagtatc tattcctaat gtaatcatat atttgatcct ttctgctatg
    16501 acattctcgc cttccattgt agcatacttt tttattctga ggatgaaaat accaaaggca
    16561 aaactagtac aataatacat gattatctgc tggcgggcaa attttgcccc ttcctgaaac
    16621 acaaaaaagc tgcggtatca ctccattttc ccacagcttt tccatctttt atttcgttga
    16681 ttctcgaata atcaactctg tttccacaat aatctttctt gaaaccggtg ctttggaatt
    16741 acttaattga agaatcgtct ccactgctag ctctcccatc cattcggtat aaacttttac
    16801 agtagaaagc gagggcgtta catatttcgc tacactgaca tcattgaagc ccatcacaga
    16861 aatatcttct ggtaccacta aacctttttc ttgaatcgct ttcagtgcgc caatcgctaa
    16921 tgcatcatta gctgcaaaaa gcgcatcagg aacatgcgga ttcgttttta aatattgctt
    16981 cactgcttga tatcctgact cgacagaaaa atctgcttcg ataattggac cttcgtcgta
    17041 gcctttttct gccaaaactt gttgaaatgc cataattctg ggatctttta gccgctgatg
    17101 gtttttcttc gtatattctg ttccagcaag catagcgata tgttgcgctt tatgggagac
    17161 aaagtgttca atgacttttt gaacacttga cttaaagtcg acaactagag agtcgattcc
    17221 ttgagaagtg ccgtcagaat ctaccaaaag caaggcattt tgggatcctt tcatttctaa
    17281 taactgagct tcgtcgaatt ttcctagtgc gatcgttcca tctgccggaa gatcactaat
    17341 ggaatcccac gtttctttca ataaagtaac gtttgcttct tctgcctttt tttcgattcc
    17401 taggcgaata gataaataat aaagatcttc aagctcttcc gtatcgtcgt accactggat
    17461 caggcgaagc gtttgcttag tatttgctgc atttttacga tgtttcgtat aatttaagcg
    17521 ttctgctgct tcaaaaattt tttgctttgt ggctaagcca acggaaagat caggatcata
    17581 gttgagaact cttgaaacag tagcaggaga aactcctgcc aactgcgcaa tgtctttaat
    17641 tgttgccatt tgtctctcct ccttttttac gtttataaaa tcgcagtaaa tcgttcaaac
    17701 gctgcctgtc ctttttcatc tcgcttgaat actccagcgt cttccagtac acgtgcaaaa
    17761 acctgaccta cagatttttg tacgacatcg gtgaccgtat ctttcgtcaa atcaggatgt
    17821 tcttttttta attgttctgc ccacggacga tgatattcag ccatctggtt ttcttctcct
    17881 aataagaaat gctctacttc tactagttct ggtttcaaac gaggtggtaa aacagcaagc
    17941 cccattactt cgatcaaccc aatgttctcc tttttgatat gttggacatc tttatgtgga
    18001 tgaaaaatcc catcaggatg ttcttctgaa acattgttgt ctcgaaggac aaggtccaac
    18061 tcaaagtaat tgtcccttct tcgtgcaatt ggcgtaatcg tatggtgtgg tgtaccatct
    18121 gctgaaaaag ctctgataga tactgtttca tccgaatatg ctttccattt ttccaatact
    18181 gagttagctg cttcaataag caagtttttc tctgttgatt gcaaacggat cacagacatc
    18241 ggccatttca cgactcctgc gatgacttgc ggataatctt ctagtttgat aagtttttct
    18301 atcggtgctt ttgccatagg aaattcatga cgtcctgcct gataatgatc atgagaaaga
    18361 atcgatcctc caacaattgg taagtcagca ttcgaaccaa caaagtaatg aggcaagact
    18421 tcagtaatcc gaagcagtct agcaaatgtt tcctttgtga ttttcatcgg gcggtgttgt
    18481 tctgataaaa tgatcgaatg ttcattgtaa taagcataag gggaatattg gaatccccag
    18541 ctttcaccgt ctagattcat tcgaataatc cggtgattgg tgcgggctgg atagttcaag
    18601 cgacctctat atccttcgtt ttccatgcaa agcatgcagg taggatagtc tacttttacc
    18661 gcctcgcgtt ctgctgcgat ttgttttgga tctttttctg gcttagataa attgattgtg
    18721 atttccagtt caccatactc cgactctgcc ggaaatttga tgttttttgc gatggctctt
    18781 gttttgatgt agtcattttt cttacttaaa ttaaagaaat actcagttgc ttcctctggg
    18841 cttttcgaat agtgctgtgc aaagaatgca ttgacgactg aaggtggcgg tgtgagaaaa
    18901 tccattaatt gcgcttcaaa aatctcttgt tctgctggca attctgaaag aacaccattt
    18961 tctcttgcct tagcgacaag ctgatccaat aagtctacag attcttcctt aactggtctt
    19021 acttcaatgc tttctagagc atcttctcct accatcccta aaagacgatt ctgtaaatat
    19081 aagcggtcca tttccatcca tccgccagct tcgatcgcaa gtgtggcaaa atcacaaatc
    19141 gtttgactga ttgacatatc tcattcccct attcttcata accatttgga tggctgacat
    19201 gccaatccca tgctgttttg ataatcgctt tgatatctgt gtattcaggt ttccagccca
    19261 gaatctctct cgctttttca cttgaagcga ctaatcggct aggatctcca gctcttcttg
    19321 gtgctatttc tgctggaatt tcttttcctg tcacttctct agctgcttct agcatttcct
    19381 tgactgagta tcctttgctg ctgcctaaat tgaagaagtt gctttcattt ccttctttca
    19441 agtattccaa agctaaaata tgtgcagcaa tcaaatcttc tacttgaaca taatctctga
    19501 tacatgttcc atctggtgta tcataatcgt ctccataaac agccaaggct tttctttgac
    19561 caagagctac ttgtaaaatg attgggacaa gatgcgtttc tggtgtatga tcttctccga
    19621 tagatgcatc tgcttttgct ccggctacat tgaagtagcg caaagcgaca tagcgcatgc
    19681 catatgcttg gtcacaccac ttcatcattt tttccatcat caatttactt tcaccatacg
    19741 gatttttagg attagctggt gtatcttctg tgattggaga ctctttcggc tctccataag
    19801 ttgctgcagt agaagagaat acgattttat tcacgttgaa ctcgtgcatg acttcaagta
    19861 agatttgcat accgtatacg ttgttgttga aatacatcaa tggtttttcg acagattctc
    19921 ctactaaaga gcttgcagca aaatggatta ctccttcaat cggttccttt tcaaatacgg
    19981 aacgtagaaa ttctttatcc cggatgtctc cttcataaaa atgagcatct ggatgtactg
    20041 cttgtttgtg acctgtgagc aaattatcta ccacagctac ttgatacccc ttttgaacta
    20101 gctggtctac agcgtgcgaa ccgatataac cggctccgcc tagtactaaa attgtcataa
    20161 aaaacactcc tttgattagt aaaattgtgt ttgttgattc tatataattt aaaaaacgaa
    20221 gtttactttt atggtgaaaa caaacaaagt aaaatttcgt aaattcatat ggtaaattta
    20281 taataaaaca tttagtaaac attttcaagg gttttttgca aaggtttaca aacaaa
//
LOCUS       DACDSY010000038        20335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDSY010000038 DACDSY010000000
VERSION     DACDSY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.038"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            3..551
                     /gene="hslV"
                     /locus_tag="IWK44_001163"
     CDS             3..551
                     /gene="hslV"
                     /locus_tag="IWK44_001163"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP9549700.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            565..1965
                     /gene="hslU"
                     /locus_tag="IWK44_001164"
     CDS             565..1965
                     /gene="hslU"
                     /locus_tag="IWK44_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP9549701.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1983..2774
                     /gene="codY"
                     /locus_tag="IWK44_001165"
     CDS             1983..2774
                     /gene="codY"
                     /locus_tag="IWK44_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP9549702.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2856..3731
                     /locus_tag="IWK44_001166"
     CDS             2856..3731
                     /locus_tag="IWK44_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9549703.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="IWK44_001167"
     CDS             complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="IWK44_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP9549704.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4628..5062
                     /locus_tag="IWK44_001168"
     CDS             4628..5062
                     /locus_tag="IWK44_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP9549705.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5228..7273
                     /gene="parE"
                     /locus_tag="IWK44_001169"
     CDS             5228..7273
                     /gene="parE"
                     /locus_tag="IWK44_001169"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP9549706.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7285..9735
                     /gene="parC"
                     /locus_tag="IWK44_001170"
     CDS             7285..9735
                     /gene="parC"
                     /locus_tag="IWK44_001170"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP9549707.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10012..12243
                     /gene="pflB"
                     /locus_tag="IWK44_001171"
     CDS             10012..12243
                     /gene="pflB"
                     /locus_tag="IWK44_001171"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP9549708.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12487..13248
                     /gene="pflA"
                     /locus_tag="IWK44_001172"
     CDS             12487..13248
                     /gene="pflA"
                     /locus_tag="IWK44_001172"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP9549709.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13333..14259
                     /locus_tag="IWK44_001173"
     CDS             13333..14259
                     /locus_tag="IWK44_001173"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP9549710.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14441..15334
                     /locus_tag="IWK44_001174"
     CDS             14441..15334
                     /locus_tag="IWK44_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP9549711.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15468..15593
                     /locus_tag="IWK44_001175"
     CDS             15468..15593
                     /locus_tag="IWK44_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9549712.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15727..16359)
                     /locus_tag="IWK44_001176"
     CDS             complement(15727..16359)
                     /locus_tag="IWK44_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549713.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16507..17049)
                     /locus_tag="IWK44_001177"
     CDS             complement(16507..17049)
                     /locus_tag="IWK44_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP9549714.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17184..17573
                     /locus_tag="IWK44_001178"
     CDS             17184..17573
                     /locus_tag="IWK44_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549715.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17577..18341
                     /locus_tag="IWK44_001179"
     CDS             17577..18341
                     /locus_tag="IWK44_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9549716.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18470..19417
                     /locus_tag="IWK44_001180"
     CDS             18470..19417
                     /locus_tag="IWK44_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9549717.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19547..20287
                     /locus_tag="IWK44_001181"
     CDS             19547..20287
                     /locus_tag="IWK44_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549718.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7139 a         3458 c         4298 g         5440 t
ORIGIN      
        1 acatggtcga atcaccattt cattcaacca ctatctgtgc cgtcgaaaaa gacgggaaat
       61 ttgctatggc tggagatggc caagtaacaa tgggtgaatc tgttgtcatg aaaggcactg
      121 caaagaaagt acgacgtatc tacaatggag aagttgttgt aggtttcgcc ggaagtgttg
      181 cagatgcgtt tactttagaa gaaaaatttg aaggcaaact aaacgaatat aacgggaatc
      241 tacaacgcgc agctgtcgaa ttagctcaag aatggcggac acagcaatct atgcaaaaac
      301 ttgaagcgat gctgatcgta atgaatgata aagaaatgct tcttgtttca ggtacaggag
      361 aagttatcac accagatgat ggtatcttag ctatcggatc aggagggaat tttgcgttgg
      421 ctgcagcccg agcaatgaaa aactatgctc aaaacgacat gagtgcgaaa gaaattgcag
      481 aaaatgcgtt aaatattgcc gcagatatct gtatctttac taatcataat attatcgtag
      541 aagaactgta agaggtgagt agatatgtat gaattaaaac aaacaccaag acaaatcgta
      601 agtgaattag atgaatatat cgtaggacag cacgcagcaa aaaaatctgt tgccgttgct
      661 ttgcgtaacc gctatcgccg tttgcagtta gatgaaaaaa tgcaacaaga tattacacca
      721 aaaaatatgc tgatgatcgg acctacaggg gtcggtaaaa cagaaatcgc acgacgtttg
      781 gcaaaaatcg tcaatgcacc atttgtcaaa gtcgaagcaa caaaatttac tgaagtcggt
      841 tatgtcggaa gagatgtaga atcaatggtt cgggatttag tagaaaatgc gatccaaatc
      901 gtagaaaaag agcagtacag ccgtgtatac acaagtgcta aaaaaaatgc agaaaaacgt
      961 ttggtaaaaa tcttggtacc tggtatcaaa aaagaacaga aacaatccac taatcaattt
     1021 gaacaaatga tgaatctttt taatgctagt cagcaaaata ccgctgaacc gcaagaagaa
     1081 gtgacagaag atattcgaat caatcgccgt acagtcttgg aacaactaca aaagggttta
     1141 ctggatagtc gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc accaatgatg
     1201 aacaatggat tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc tttgacaccg
     1261 aagaaaaaag tggaacggac ggtaacagtc aaagaagctt tagagttatt gatcaaggaa
     1321 gaatcagcaa aattagtcaa ggatgctgat atccatagtg aggcaatcaa attagcggaa
     1381 gaaagtggaa ttatttttat cgatgaaatc gataagatca cttctaaatc acagcaatct
     1441 ggagaagtat ccagagaagg cgtccaaaga gacatattgc caattgtcga aggttcacaa
     1501 gtaaacacaa aatacgggat catccaaaca gatcacgtcc tattcattgc ttcaggagct
     1561 ttccatttgt ctaaaccaag tgatctaatt ccagaacttc aaggacgctt cccgatacgg
     1621 gtagaattag atgatttgac agctgaagat tttgtgaaga ttctgaccga accaaataat
     1681 gctttagtaa aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt tacgtttaca
     1741 aaagaagcaa ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga cacagataat
     1801 atcggcgcac gcagactgca taccattttg gaaaaactac tagaagatct attatttgaa
     1861 gcaccagata tgcaaatggg agaaataacg atcacagaag catatgtgga tgacaaactt
     1921 ggctcaattg cggcaaatga agatttaagt cgctatattt tataaaaaat cggaggacga
     1981 caatgacaga cttgctaacg aaaacccgca agataaacac acttttgcag caaaagaata
     2041 cttttgacat gcaagcagat ttgccttacg ataaaatggc tgtcacacta ggtggtattc
     2101 ttgacagcaa cacctatatc ataggaagtg acggagtatt actaggttat aatgaacgtc
     2161 acgatgtgaa caatgaacga atcaaaacaa tgtttgttga gaaacaattt cctgaatctt
     2221 atactgatac agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt actagtgata
     2281 ttactgcatt tccggtagag tcaagagaga aatatccatt tggtctgacg actgtcgttc
     2341 cgatctttgg agctggcgaa cgtttgggta cgattatctt atctcgtatg gaacaatcgt
     2401 ttgcagatga cgatctagtc ttagcagaat atggagctac ggttgtcggt atgcagatcc
     2461 tttatcagaa gtctcgaagc atcgaagcag atgtacggag tgcaacggct gtccaaatgg
     2521 caatcaacac attatcttat agcgaattga aggctgtaca agctatcttt gaagcattgg
     2581 atggagatga aggtcgtctg actgcttcta atattgccga tagtatcggc atcactcgtt
     2641 ctgtgatcgt caatgcttta cgaaaattgg aatcagctgg aatcattgaa tctcgttcgt
     2701 taggcatgaa gggaacttat ttaaaagtat tgaattcccg ttttaaagac gagttagcaa
     2761 aacacagtta ttagaagtaa ggggactggg gcagatagaa atgtttgttc cagccttccc
     2821 attttaaaaa gtaagtcgaa gttggaggaa tggcaatgac tgttattatt caaaatgacc
     2881 agctgatcgc cgaaatcgct gaacacggtg cggaattgat cagtctgaaa tcaaaagaac
     2941 atggtttaga gtatatctgg caaggtgatc cagcgtattg gggcagacat gcaccggttt
     3001 tattcccttt tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg aaaacatatc
     3061 aaatggggca acacggattt gctcgggata tggattttga agtcattgaa catggacaag
     3121 aaatggctag tttcatctta aggagtacgc cggagacatt agaaaagtat ccatttgatt
     3181 ttgaattagt gatcagctat gaattaggag gagacggcat cacggttcat tatcaagtgg
     3241 caaataccgg tgaaaaagaa atgttctttt ctatcggtgg acatcctgct ttcaatgtac
     3301 ctcttgaaga aggattgtca tttgaagatt tctatctggc attttctcca ttgaaatcaa
     3361 gattacgcct accattgaaa ggcccattca tcgatatgga tcaaaagacg ttaggtcaga
     3421 caaatacgaa tctggctttg aatcgtgaac tttttgagca agatgccttg gtttttgaaa
     3481 caaggggtct aaatgcgtac gccattcgaa gtgaaaaatc ttcccatagc gtaactttaa
     3541 gctataaaga tatgccttat gtagggatct ggtctcctta tccaaaagaa gcgcctttcg
     3601 tttgtatcga accgtggtgc ggaatcgctg acacggtgaa cagtagtgga aacttgatgg
     3661 aaaaagtagg gatcaatcaa ctaggtgccc atgaaatctt caagacaaaa tattccatca
     3721 cagtgaagta agaggttgtg accgagaagt tagcagggaa caccgtttac caaggtgtgt
     3781 aaaaaagcta tcctgtaagt aataagggag tgtgacatcg atttgtcaca ctcccgtttt
     3841 atctctttgg aatgaaaaag tatcaggatt ttttcttgct gctattcaaa ccaaacggga
     3901 ttcgactttc agtatggttt ctgatacgct tgatattgtc tttatgccga taaaagataa
     3961 aggaagaaac taaaaaggca atgatcgtca gtaaccaatt gggcttagcc aaaatcacag
     4021 gagctacata aggaaggacg atcgtagata aagtgatcaa tactgcactg atcatactgc
     4081 tcaaactcac catacttgta caaaataaac taacgataaa gatgatacaa gaataaacaa
     4141 aaaatacagg attaaatcct aaaagcattc ctgcacttgt cgcaacagct tttcctcctt
     4201 tgaacccagc aaaaatcgga aatgtatggc caagaatggc gcatacccca aaccaaagag
     4261 ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt agccaatgta ccttttaaaa
     4321 tatccataag caggacaaca atcccggctg ttttgcctaa tacacgaaat gtattggtcg
     4381 ttccagtatt accgctcccg tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc
     4441 agacaccgga aggaatagaa cctaaaagat atgctaaaat aagtaataag atcaatttca
     4501 ttttttaccc tctttcaagt tgaaaactat cctttatttt agcatgaata ggtaacagga
     4561 acaattgaaa aaatgaaaga tacaagtata atagatggaa taaaaagaaa agcaaggggt
     4621 ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt ttcaactatt tggaagaagc
     4681 aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa cgaacaagtt acatggttgc
     4741 acaggtattg caagacaatg gatataaaat cataccagta aatccattat tggctggtga
     4801 agaaattcta ggcgagaaag tctatgcgaa tttgacagaa atcgatgatc caatcgatat
     4861 cgtagatgta tttagacgga gtgaattttt acctgaaata gcaggtgatt ttttgaaaac
     4921 tgatgctaaa gtgttctggg cacaattagg gattgaaaac aaagaagcag ctgagatgct
     4981 aagaaaagca ggacgtacag atatcgtaat ggatcgttgt attaagattg aacttggtaa
     5041 gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga gatgatcgat agaggggaat
     5101 gtcgaaaaag cggttaggag ataattaaaa ttgatttctc gtctaccctc ttttttttca
     5161 cgaccttttc tagtatgata atcgtggtgc ggttttgccg caaagtatta ttgaaggagc
     5221 gtttgccttg gcaaaaaaag taaacaacga atacaatgac tcgtccatcc aagtattgga
     5281 aggattagag gcagttagaa aaagaccagg gatgtacatt ggatccactg acagccgagg
     5341 cttgcatcat ttagtctatg aaatcgtcga taatgcagtt gacgaagcat tatcaggtta
     5401 tggcagtgag attgatgtga ccatccatga agacaatagt ataactgttg cagatagcgg
     5461 acgtgggatg cctgtaggaa tgcatgcttc cggaatacca acagttgaag tgatttttac
     5521 tgtgctgcat gctggcggaa agtttggtca aggagggtac aaaacttctg gtggactgca
     5581 cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag
     5641 agatggcgta gagtatcagc agaaatttaa aaacggcggt aaacctgacg gtactttgaa
     5701 aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc cattttttac cagatgatac
     5761 aatattcagt acaacgaaat tttcatatga aattttagcc gaacggttaa gagaatctgc
     5821 ctttcttcta aaaggcgtta aaatctctct ttctgatctt cgtggagaag aaccagtcaa
     5881 ggaaattttt cattacgaag aaggaatcaa agaatttgtt gattatctga atgaagaaaa
     5941 agatacattg acaccagttg tttatttttc aggagaaaaa gaaggtatcg aagtagaggt
     6001 cgcttatcaa tacaatgacg gttattcaga aaatgtttta tcttttgtca ataacgttcg
     6061 gacaaaagat gggggaaccc atgaagctgg tatgaaagcg gcgatgacga aatcttataa
     6121 tgaatatgcg agaaaagttg gtttgctgaa agaacgggac aagaatctgg aaggtagtga
     6181 tttcagagaa gggttagctg ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt
     6241 tgaagggcaa acaaaagaaa aacttggtac gccagttgct cgtaccgtag tagataacgt
     6301 catcagtgaa caaatgggct tttatttgca ggaaaatagc gaaatgagcc agatgttggt
     6361 ccgtaaagca atcaaagcca gagaagcgag agaagcagca cgtaaagcca gagaagaaag
     6421 ccgaaatggg aagaagcgta aaaaaggcga atctcttctt tcaggaaaat tgacacccgc
     6481 acaatcccga aaccctaaga aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg
     6541 atctgctaaa caaggaagag acagaaagtt ccaagcaatt ctacctttac gaggaaaagt
     6601 catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat
     6661 gatctatacg attggtgcag gagtcggtcc tgagttttct atcgaagact gtaattacga
     6721 caaagtaatc atcatgaccg atgcggatac agacggcgcc catattcaag tattactgct
     6781 tactttcttt tatcgttata tgaaaccgct gattgaagct gggaaagtat atatcgcgtt
     6841 accgccttta tataaagtat ccaaaggaca aggaaagaag caagtcattg aatatgcgtg
     6901 gacagatgac gaattggcag caatgattaa aaaagtcggc aaaggatata tgctgcagcg
     6961 atataaagga cttggagaga tgaatgcgga acaattgtgg gaaacaacga tggatccgac
     7021 ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg caagctgaac gtcgagtaac
     7081 cacactgatg ggtgacaaag tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt
     7141 tacgctcgaa gaagatggca gtatcttaga taaaaaagag gatactgaaa tctctccttc
     7201 agtttctaat gatttattag atgaagaacg agcagataaa aatgaaaaca atcagttatt
     7261 tgaagtagaa taggaggtca cctgatggaa aatcgacaag aagtacaaga attaacttta
     7321 gaagaagtga tgggtgaccg cttcggacgt tattccaaat atattatcca agaacgtgca
     7381 ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc ggatattatt tgccatgaac
     7441 aaagatggca atacgtatga caaaggattc cgtaaatcag caaaatctgt cggaaatatc
     7501 atggggaatt atcatcctca tggtgacatc agtatttatg aagcgatggt ccgcatgagc
     7561 caagactgga agttgcggga agtactgatc gaaatgcacg ggaataacgg gagtatggac
     7621 ggtgatccac ctgctgcgat gcgttatact gaagccagat tatccaagtt aagtggagag
     7681 atgctagcag atatcgagaa agaaacagtc gatctcgtgt ggaattttga tgatacagaa
     7741 aaagaaccaa cagtccttcc agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt
     7801 atatctgctg gatatgcaac agaaatcccg actcataact tagctgaagt gatcgatggc
     7861 acaatctata tgatcgatca tccacaagcg agccttgaaa aattgatgga atatattcct
     7921 ggacctgact ttccaacagg agggatcttg caaggaaaag atgaaatcaa aaaagcttat
     7981 gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa agatagaggc aatgaagggt
     8041 aataaacagc aaatcgtgat cacagaaatc ccatatgaag taaataaagc gaccttggtg
     8101 aaaaaaatgg atgagatccg tttgaataag aaaatcgatg gaatagctga agtacgagat
     8161 gaaagtgata gaacaggtct gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa
     8221 ggaattttga attatttgtt caaaaacact gagctacaga tcaattacaa tttcaatatg
     8281 gtcgctatcg accatatgac tccgcatcaa gttggactga aagacatttt aagaagttat
     8341 atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat tcgatttagc caaagctcaa
     8401 aaaagacagc atatcgtaga aggattgatg aaagctttat cgattctgga tgaagtaatc
     8461 gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt
     8521 caatttacag aagaacaagc agaagcaatt gtcacattgc aattataccg tttgacgaac
     8581 acggatatca cagagcttca aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg
     8641 aataaaatcc tttcaaatga caaagagctt ttctctgtta tgaaaaaaga attgcgagaa
     8701 gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc
     8761 aaaattgata cgcaagtctt agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa
     8821 ggctatgtga agcgaacaag ccttcgttct tatagtgctt ccaaaccaga agaaatcggg
     8881 atgcgagaag gcgactactt gctatactca ggtgaattga gtacattgga ccatgttctt
     8941 ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc atgaactacc cgatttgaaa
     9001 tggaaagatg ctggtgagca catttctcaa acaatctcca atctgtctgt agacgaatcg
     9061 attttagctg ttttcccata tcaaaaaatt caagcagaga aaacatttgt attcatcagc
     9121 aagaatggat tgatcaagca aacacgaatg acagagttcg aaccatggcg cacctataag
     9181 agccgtccgc tcagctgtat gaagctaaag gcggaagaag atgaactgat tgctgtgtat
     9241 ctgaaagaag atcagccagg cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg
     9301 cgctatcctt taacagatgt tcccgttgtt ggagcaaagg cagctggagt gaagtctatg
     9361 aatttgaaag atggagatag gattatcaac gggctgcttg tgttagcaga aggtgatacg
     9421 ccgatcgtaa tcgttactca gcgaggagct gttaaacgca tgcttgctca agaaatcagc
     9481 caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac
     9541 cgtattatct atatgtcgga aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg
     9601 cagcagttaa cgattgaccc aactgatttc cctataggtg atcgaacatc gaatggatca
     9661 tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt
     9721 ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga cgccatctgt attaatacag
     9781 gtggcgtctc tttttcattc atttttaata aatccttata cgtttgcaaa aaaagatgaa
     9841 agtgttcaca atgcttaaat gttggtatgt caacgttgat ttaaatatat gtaaataaaa
     9901 cataaaaatt gttaaataaa aaacaagaaa acccttgcaa agatagggtg ctgtgttata
     9961 ctaatcacgt aaaaactgtt acataaaaca gaaatttagg aggtccatac aatggagcaa
    10021 tggaatggat ttaaaggtac caaatggaaa gaaggcgtag acactagaga ctttattcaa
    10081 aataactaca ctgaatatcg cggtgatgac agctttttgg aaggcccaac agaagcaaca
    10141 gaaaaattat gggatgaatt gcaaaagtta aatgatatcc aattcgagcg taacggtgtc
    10201 tatgatatgg acacagaaat cgtatcaact attacttcac acgatgcagg ctatttggat
    10261 aaagatttgg aaaaaatcgt cggtctacag acagacaaac ctttgaaaca agcattcatg
    10321 ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac
    10381 gatgaattga cacatatttt cactgactgg cgcaaaacac ataatcaagg tgtatttgac
    10441 gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg gcttccagac
    10501 gcttacggtc gcggacgtat catcggtgac tatcgtcgtg tggcattgta tggaatcgac
    10561 tatttgatgg aacaaaagaa aaaagatcat gatatgactg gttatcatac aatgagtgaa
    10621 gaagtgattc gtttacgtga agaaatcact gaacaatatc gtgcattagc gcaaatgaaa
    10681 gaaatggctg cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct
    10741 gtccaatggc tatacttcgg atatttagca gcaatcaaat cacaaaatgg tgctgcgatg
    10801 tcaatcggac gtatctcagc attccttgat atctatatcc aacgtgattt ggataatgga
    10861 gtaatcacag aaaaagaagc acaagaattg atcgaccact tagtaatgaa attacgtatg
    10921 gtcaaattcg cgcgtacacc tgaatataac caattattct caggattccc tatctgggct
    10981 actttatcca ttgcaggtat gggattagat ggtcgttcat tagttacaaa gaatgatttc
    11041 cgtctgcttc atacattaac aaatatggga ccttcaccag aaccaaactt gactgttttg
    11101 tattctgaac accttccaga aggattcaga acatatgcta gcagaatcgc tatcgaaagt
    11161 tcttcgattc aattcgaaaa tgataatcag cttcgtgaac aatggggttc tgatgattgt
    11221 gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt ctttggcgct
    11281 cgtgctaact tggctaaaac actattatat gccatcaatg gcggagtcga tgaagtgact
    11341 ggcgcccaag ttggaccaga atatcgtcca atcacatcag aaatactaga ctttgaagaa
    11401 gtgaaacata acttcatgaa tatgatggat tggttagcag aactatatgt caatacgttg
    11461 aacatcattc attatatgca tgataaatat tcttatgaag cagctcagct ggctttgatg
    11521 gattctgact tgcaacgcac atttgctaca ggtattgctg ggatctctca tgcggcagac
    11581 agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca
    11641 gttgattttg aagtggatgg cgacttccct aaatacggaa atgatgatga ccgtgcagat
    11701 gaattagttt catggatctt ggaatacttc atgggacaaa tcaaacgtca taaaacttac
    11761 cgcaactcaa aaccaacaac ttcattattg acgattactt caaatgttgt ttacggtaaa
    11821 aatacaggaa atactcctga tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat
    11881 ccaagttaca atgcggaaca aagtgggtta ttagctagcc tgaactcaac agctaaaatg
    11941 aattacggct gtgcattgga tggaatttca aatacccaaa caatcaatcc ttcaggtctt
    12001 ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg atacttctca
    12061 aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg
    12121 gaaaatccaa tgaaatatcc gaacttgacg atccgggtat ctggttatgc agttaaattc
    12181 cgtgacttga caagagaaca acaattagat gtcttgatga gaacttcaca cgacagaatg
    12241 taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga atagccatcg
    12301 catcaaaatc gaataatcag tatgcttatt tgaaagaaac gatttacagg ggcaggactt
    12361 gtatgttccg tccctgtaaa ttttacacat aacgaagcgg caattgcgaa aaattttgtt
    12421 cagaaagctt cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga aaagatgtga
    12481 acttaaatgg aagaaaaaac aatcggctat gttcattcta tcgaaacttt cggttcagta
    12541 gacggacctg gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt
    12601 tgtcacaatc cagatacttg gaatattggc ggagggaaag aatacacagc agatgaactt
    12661 ttagataaag cagaacgctt cagaccttac tggggagata aaggtggtat tactgttagt
    12721 ggtggagaac ctctattgca gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag
    12781 cggaagatgc atactacatt ggatacatgc ggaaaacctt ttacgtatga agaacctttc
    12841 ttttcacgtt ttcaagaatt gatgaagtat acagatttac tgttatttga catcaaacac
    12901 atcgataatg aagagcataa aaaactaacg cattgggaca atgaaaatat tttagaaatg
    12961 gctcaatatt tgtcaaaaat caacaaaccg gtttggattc gccatgtact agttcctgag
    13021 cgaagtgatt atgatgaata tttgatccga ctagacaact ttattcaaac cttgtcaaat
    13081 gtagacagag tagagattct tccttatcat acgatgggaa aatacaagtg ggaaacactt
    13141 ggtttgaaat atccattaga agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct
    13201 aaacgattgc ttcatacaga agagtataca aaatatttaa cgcgataggt cgcatcggaa
    13261 agaagataag tattgcttct ttccgttttt ttgctattat taaataggag aaatcacaaa
    13321 ggagcgaatg acatgtcaaa aattttagtt tttggtcacc aaaatccaga tacagatgca
    13381 atcggggcag ccattgcgtt ttcacacttg caaaatgaat taggtaaaga aacagaagca
    13441 gttgctctag gtacacctaa tgaagaaaca caatacgcat tagaccactt tcagctatct
    13501 gcaccacgag tagtagagtc agctaaagca gaaaatgcag atgtcatgct tgtggatcat
    13561 aatgaattcc aacaaagtat ttcagatatc agcgaagtaa atattttagc tgtagtagac
    13621 caccatcgta ttgcaaactt tgaaacagct aatccattgt actatcgtgc agaaccagtt
    13681 ggatgtacaa gtacgattat tttaaaacta tataaagaaa accaagtaac aattcctaaa
    13741 gaaatcgcag gaatcatgct ctcagctatc atttcagata cattgttgtt caaatcacca
    13801 acatgtacgc ctgaagatga agcagctgca aaagaacttg cagcaatcgc tgaagtcgat
    13861 ctaaacactt atggacttga aatgttgaaa gcaggaacaa atctaagtga taaatcagct
    13921 gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc
    13981 gctcaagtca atacagtgga ccttaatgaa gtgatggatc gacaagcaga gctagaagca
    14041 gcaatggaag atgaaatgaa agcaaatggc tacgatctgt tcgtattagt tgtcacaaac
    14101 atcttagaca gtgattcaga attacttgtt gctggtaaag gaaaagaaaa agtagaagaa
    14161 gctttccaaa caactttgac caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa
    14221 aaacaagtcg tacctcaatt gacggctgta tttagctaat caaatggtag tatgaagcat
    14281 tcaaaatgaa tggataagcg attgatataa aaagaaacat agaacatact aaaggccgaa
    14341 gaaatggagg agaataacag tcaattatga ctcttgttct tcctcctttt tacgtaaaag
    14401 ccttttaagt aaaataaaac ggatagaaaa gagaacatat atgaactatt attcaaaaaa
    14461 taaggaaaac ttttatcaaa aactgaccga tgatccgctt ttttcttcat tgaccgatta
    14521 tctatatgaa catcgagaac aagaaacgat tctaagagaa ttgaaaaaag aattttcgca
    14581 aaataaattt tcccattttc ttgatttatt gatcgatgca ggattaatca agagggaaga
    14641 acgaagatat catttgaact ttccaatatt tgatcctaaa gattacttgc aacaagccac
    14701 ttcggctgct gaaaccatag ctgaacaatt gaagagactc tctgttgatg aacaaaaatt
    14761 agcgatggga gaagttattt gggcgtattg tttcgaagat gaacggaaag aagcgtattt
    14821 ttatggagta agaaacagcc gagagacaga attgctgagg acaactgcag gaaatgagaa
    14881 gtaccgtttt atcacacttt cctctataga acacttcccg cttactttgg ctaattactt
    14941 ttttgtacag aagaatcagc ttcctgtaac gaaggcattc aaagaattag ccgaacttat
    15001 tggagacgta aatgaatcgt acttttttga tcaaatagaa gttatcgttg acaggatacg
    15061 taagaataaa tataaaaatc gccggcctag tattttccat cagtctctac ttgtgacgaa
    15121 tactataaag gaagaagaat cattcacatt agaattacct attgtcgaaa aaaacaattt
    15181 tgagattgaa ttaccaacat tggatccttc attgacaatg gaggaaatgg cctttcttaa
    15241 acgacaaatc ttttctgagc tgagcaagaa atttataccg catgcatttt cttatatcaa
    15301 agaatatgga acagttttag tgagtaaaac ctaagagagc agtaagcaaa aattatactg
    15361 actcttgcag gaggagcctt tctcttcctt tatggttaaa agaattattt gagtattttg
    15421 cctatctacc acctattaga ttcactacat ggtctggaaa caaaggaatg caaaaaaatg
    15481 gtgagaaatg tggaatgaca aaggaagtag tgataagaaa agttagattt ttaaataatc
    15541 aatattatga ttcagtcaag tatggcattt tatgggaaga actagctgat tagattagga
    15601 agtccatttg ataatccaga atcactaaga aacgaatggc gagtcattaa taatttatcc
    15661 aatcttgggt tattctttta gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg
    15721 taatagtcag gcttcaaaat gctttttatt ttgtttgttt tttctttgcc acataaatac
    15781 acctagactg acgatgaaga gcatatccaa cagatttcct gcgacttgga tcgtcgaagg
    15841 gttggtcagt ggcattaaag caaggacaaa aaaggaaaac aagctgtaaa taaaacaggt
    15901 gacaggaact aatcgaaaga agagcggaca tttcaattgg ctaaaaatga gtgaaaaaat
    15961 aaaataagta aaaatggata aaatcacgac taaaccattc atgataaaga agaaaagcgt
    16021 attgtgatta aacgaataaa aaagaattgc taaaaaagaa aacgttttaa atgcaatcaa
    16081 tacgcttgaa gcttcgttta acgtaccagg atgtcgatga aaaaaatagg caccgaaata
    16141 aacagaaaac agtaagccgc aaagagaaga caaaatcaaa aaaaacggat tttcttcggc
    16201 ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat gcactagaag gcgcatgtga
    16261 ggtcttaagg tatgaataac atagaaaaaa taagatcgtc aaagttgcaa aaaaagagcc
    16321 taaatgtaaa aaattagtgg ttctacgttg aatttccata atatctccaa attaaattga
    16381 taagtatata aagtataata acaaattgat gagtatatgt cgatgttttt taatcaaaat
    16441 aataaataaa aactgtctaa catttaaaat ttaatttaga tattagacag tttttaagtt
    16501 tgtgagttat tttccttgtc gttttctcac ttttttagtc cagaaaccac ctgaaatata
    16561 ttttggctca taagaaatga taaaagcacg ctcttcgatt tctttgatca ggcgatacag
    16621 ttcacgttcg ctttttctag gagaaagtat ttcgagaact agccgctcac cttccagccc
    16681 cattgcagaa gtctgtgtca caccataacc gtgttctctc agtatacccg gcaaactttt
    16741 atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg atgtatccta atgccaattt
    16801 gtcttcaatc aaaattccga cactgatacc tactgcgtaa cctaacgcat agacaaataa
    16861 attcagtgga ttatctaaac gatctaaaac aagggataag ccgacgatat aaatcgtgat
    16921 ctcgaccata ctgacaagtg gagcaatcag tcgatagcct ttcattgtca acatgaaacg
    16981 taacgtgttc agagtgatat aagcaaaatt aatgaggaag atcatcccca acatttttgg
    17041 atcaatcatt tgtaaaactc ctttcattta tataacaaca atatcaaaaa aaatcgtaag
    17101 tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata aaatgattat aatagaatta
    17161 taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg
    17221 tgctaagtct ccttctcagt atatacttag gtgtctattc gattcaacag ccaattgagg
    17281 tagagaacat tgtttttcag atgtcgctga cagatggttt aattttaagc gtttttttct
    17341 ttttgatact gtttattggt aatattttcg gtagcgttgc cagttttttg aatttttcta
    17401 tttatccact cctttcgcta gcattaggag tggtgggact ttgttctctg tgcttatttc
    17461 ccgagccatt cagtcctgca tttatattat ttggtacact gaacctgttc cagacaactg
    17521 ttggagcatg gttgctatgg cgatcaggaa atttaatgaa aataggtgag taacaattga
    17581 atccaattat attgaaacgt tctgtccatc atattccagt gttagaagtt gttctgaaag
    17641 aattaagaga ggaacgtatt cccgttgtca tctattatca cggatggcag acaagtaagg
    17701 agttagtttt gacgcaagga agaaaaattg cgcagcaggg cattcgtgtg atcctgccag
    17761 atgcgatgaa tcatggggag cgcaaacagc cggtttcttc tattccatcc tatacatttt
    17821 gggatagtat ctacggaaat ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa
    17881 gaggtcttct acatgatcag cttgcagttg gcggggtttc gatgggggga atgacaacat
    17941 gcgccttgtt ggctaaacat cctcatatag tagcgggcgt gtgtttgatg ggttcccctg
    18001 cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac
    18061 caaaagacta ttttgatttg atttcatggg ttaagagcta tgatttgtca ttgcagccgg
    18121 aaaagttagc tggacgtcct ctgttctttt ggcatggaca agctgacgaa aaaatccctt
    18181 atatacaaac ggaacgattt gtagaaaaaa atcggcataa gtcttacgga aaagaaattg
    18241 tttttcgttc ctcaaaaaca cagcgacata tggtacaaat accactaatg gaagaagctt
    18301 ccactttttt agcaaaaaat ttgataaaaa agtataaata attgaaacgg aaaatcactc
    18361 atgacgatag aggattttcc gttttttatt ttgggctata ataaactctg ttaggaaaat
    18421 ttggtataat tgatagtaag aatgacaaca tgttagaaag gaagagagca tgcccacttt
    18481 agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg gtctctcgag tcatcaatca
    18541 tccggaacag gtgactgacg aattgaaaga gttagttttc tcagcaatgg ctgaactgga
    18601 ttaccgtcca aatattgctg caaaagcact tgtcacgaat cggtcgcaga tcatcaaatt
    18661 atttatactg gaagaaatcg acacaactga gccctattac atgaatttgc tgatgggaat
    18721 cgctaaacgg atcggtaaag cgcattattc tctccaatta gttacaaatg atgccttcga
    18781 cgtcggttct tgtgatggat acatcatcac aggtgtcaga aaaaaagact tcgattggat
    18841 cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat tctgaaggat atgattatgt
    18901 agacagcgat aatgaatatg gtacagcaaa agcaacgcaa tatgccttag aaagagatta
    18961 tcaaacgatt atttatatcg gtatcgaagt ggacgagcct tttgaaattt caagggaaac
    19021 tggttattta tctgtgatga aggaagttga tcttcctagt aaaatcttgc ggtttccaaa
    19081 tcattcgaca gaagtagaaa gatatatgac agaaaactgg gcagatttta cagataaaac
    19141 ttgttttgta tgtagttcag atcggctagc actaggagca gtaagaggga ttataaatgc
    19201 tggaggacac ttgcccgaag aatttggtgt gattggattt gatggggtat ttttagacca
    19261 aatcagttca cctaaattga caacgatcaa gcaagatatt atacgtatgg gtgaagtatg
    19321 tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg agtcaaggtt atcgtcgttt
    19381 ttttcctgaa ctagttatca gagagacaac gaattaagtc ttattactat atatatattt
    19441 atgaaattca tcgctatttt cttaaaataa tcagcgcatt gctactgaaa acaatgaatc
    19501 tgtgctatta ttgagaaaag ttccattttt gagaaggatg gtttgaatga aagaaatcag
    19561 acgaaaacat ttccgttttc ctgcgtacga tgatgagtta ggcgtaaaat taaccaacga
    19621 caagaagcga gtattatttg acggacaaga agatcttttg actgaacaga atcagtcccc
    19681 gtttgaacag atgaaagaat ttcatttcac gaacacgaac accgagaaaa tccgtcgcaa
    19741 gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag gaaaatttgg aaaagcataa
    19801 aggaaaatta ccggattata ccggccatta ccaatcagaa atcacatcga caggacgaaa
    19861 aaaacttttt ggtgattcct ctcgttctgt cagacaaagt gaaacaagga cagagtccaa
    19921 gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg gagcgttctt attttgttcc
    19981 taaatatgta cccgcatcga ttatcccaga ccctagagaa agcaaaattt ctgaggatca
    20041 tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg ttgttcgata cagaaccgac
    20101 gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga acattcaatc gaacaaaaac
    20161 gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa aaaagccatg gtatcttaga
    20221 acgctcatta caaggattga tcgatgatca aggaaactct ttgggagaaa atagctattt
    20281 taaataagtg atatacaaat ttatattcgg ctacagttgg aaaggaaacg ctgcg
//
LOCUS       DACDSY010000039        20328 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDSY010000039 DACDSY010000000
VERSION     DACDSY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.039"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..456)
                     /locus_tag="IWK44_001182"
     CDS             complement(<1..456)
                     /locus_tag="IWK44_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9549719.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
     gene            complement(561..1424)
                     /locus_tag="IWK44_001183"
     CDS             complement(561..1424)
                     /locus_tag="IWK44_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9549720.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1620..3719)
                     /locus_tag="IWK44_001184"
     CDS             complement(1620..3719)
                     /locus_tag="IWK44_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9549721.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="IWK44_001185"
     CDS             complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="IWK44_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP9549722.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5072..6913)
                     /locus_tag="IWK44_001186"
     CDS             complement(5072..6913)
                     /locus_tag="IWK44_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9549723.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7095..9488)
                     /locus_tag="IWK44_001187"
     CDS             complement(7095..9488)
                     /locus_tag="IWK44_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9549724.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9595..10455)
                     /locus_tag="IWK44_001188"
     CDS             complement(9595..10455)
                     /locus_tag="IWK44_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP9549725.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10514..11035)
                     /locus_tag="IWK44_001189"
     CDS             complement(10514..11035)
                     /locus_tag="IWK44_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP9549726.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11355..12074
                     /locus_tag="IWK44_001190"
     CDS             11355..12074
                     /locus_tag="IWK44_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9549727.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12100..14691
                     /gene="mprF"
                     /locus_tag="IWK44_001191"
     CDS             12100..14691
                     /gene="mprF"
                     /locus_tag="IWK44_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9549728.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="IWK44_001192"
     CDS             complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="IWK44_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9549729.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16434..16790
                     /locus_tag="IWK44_001193"
     CDS             16434..16790
                     /locus_tag="IWK44_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9549730.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(17031..18035)
                     /locus_tag="IWK44_001194"
     CDS             complement(17031..18035)
                     /locus_tag="IWK44_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP9549731.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            18201..19052
                     /locus_tag="IWK44_001195"
     CDS             18201..19052
                     /locus_tag="IWK44_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9549732.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            19069..19314
                     /locus_tag="IWK44_001196"
     CDS             19069..19314
                     /locus_tag="IWK44_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549733.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            19501..20205
                     /locus_tag="IWK44_001197"
     CDS             19501..20205
                     /locus_tag="IWK44_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549734.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         5746 a         4168 c         3560 g         6854 t
ORIGIN      
        1 tttttctttt tcatagcatt cctgacagat tccatggaac gttagccgat gatcttttac
       61 taaaaaatga taacgttctt ctactacttg ctccacttca agtaaaagat cctcttctac
      121 ttcctcgatc gtgccgcagt tcaagcatag taaatgatga tggaaatgtt tggcgccttc
      181 ttttctcaaa tcataacggg ctaatccatc attaaaactt actttatcga caattttcaa
      241 ttctgtcaag atttcaaggg ttcgataaac agtagcaagt ccaatttccg gactcttttt
      301 tttcactaaa aagaagattt cttctgcaga taaatgctct ttctcatttt ccaaaagcac
      361 aagcagagta gcttctcttt gcggtgtcag cttaaacccc gcttcatgta gctgctgttt
      421 tgtttttttc atcgccgaaa tagcattcat gaccatttac tcaaccccaa caatttataa
      481 taattacaaa taaaaaaaga tataacacta tgttaattaa aattattata agctagttaa
      541 gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac accgttttct
      601 tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc tcctttgctg
      661 ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt gatttgctga
      721 tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat agcttcatat
      781 gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat cactcgacct
      841 tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat aaacccaaga
      901 tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc tgttcctatc
      961 acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa tattttttca
     1021 tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat catcaagcga
     1081 tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga tacgacaaag
     1141 tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt agcagtaacg
     1201 attccaaaag cgtaatgacc gatacggctt ttaggaattt gtgtagtgat ccgattttca
     1261 tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg ttcaccttct
     1321 gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt ttcattttca
     1381 tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt ctccctctat
     1441 agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat ctacttgaat
     1501 agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt gtcatactcc
     1561 cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt tgcctaaaat
     1621 taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac gatgtaaaat
     1681 ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt ggcatataga
     1741 ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc tccttcaacg
     1801 atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg tgcccacgtc
     1861 catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat tggctgattc
     1921 gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc
     1981 gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt tgcttgttct
     2041 tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt tttctcttta
     2101 tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga tagatctgta
     2161 tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc
     2221 actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg caattcagca
     2281 tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc
     2341 gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc tagatgatca
     2401 tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg gatcttagat
     2461 tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt aagactggat
     2521 aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat aaattgatgg
     2581 tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg ttgctgtctt
     2641 tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata agcccagctt
     2701 cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt tgattttttc
     2761 ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt tttttcagta
     2821 tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg gttctcttct
     2881 ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt ttcagcatcg
     2941 gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc gagtatttct
     3001 tttttctctg cttccggcag tttattcgtt ttttcctttt ctaattgttt ttcagtaaat
     3061 acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt ttctttacct
     3121 gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg ctctactttg
     3181 ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg ctcttctttt
     3241 gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc ctcattatcg
     3301 acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt ttcttcttga
     3361 ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc atttttctct
     3421 atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc tttttcgaat
     3481 aattccatta cagattctag aggtaaatct ttcaattccg accattcgat tggatcgttt
     3541 aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc tgcatctgta
     3601 tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg atgaccgcag
     3661 tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca ttttttcatc
     3721 acttagcgat cggctccttt actaggttta cgctaccatt taatctatca tattctatac
     3781 gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat ttccttccta
     3841 tagaaaaaaa agcttgagac tgcgtagtct caagcttttt ttcttttaaa ataaaccaaa
     3901 cagttttact gatttctctt ctggtcaata gtccacgctt attctaagaa atcttttaat
     3961 tgttttgaac gagaaggatg gcgcaatttt cttaatgctt tagcctcgat ctgacgaata
     4021 cgttcccgag taacaccaaa tactttccct acttcttcca atgttctcgt ccggccatca
     4081 tctaaaccaa aacgtaagcg aaggacattc tcttcccgat cagtcaatgt atctagtaca
     4141 tcttccaatt gttcttttag taactcataa gctgcgtgtt ctgctggact tgttgcttcc
     4201 tggtcttcta tgaaatcacc taagtgagag tcatcttctt caccgattgg cgtttcaagg
     4261 gaaacaggtt cttgcgcgat tttcaagatt tctcggactt tttctgttgg aagatccatt
     4321 tccgcaccga tttcttctgg cgtaggttct cttccaaggt cttgcagcaa ttgacgttgg
     4381 atacgtatca atttattaat tgtttcaacc atatgaactg gaatacggat cgtacgtgct
     4441 tggtcagcga ttgctcgagt gatggcttga cgaatccacc atgtagcata ggttgaaaac
     4501 ttaaatcctt tgcggtagtc aaatttttca actgccttca tcagtcccat gtttccttct
     4561 tggatcaagt caaggaactg catgcctcgt cctacataac gtttagcgat acttactact
     4621 aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt ggtcgccttc ttcgattttc
     4681 aatgctaacg ctacttcttc ttccgcagtt aaaagggaga cacggccaat ttctttcaga
     4741 tacatacgaa ccgggtcatt gatttttaca ccggttggcg cagaaagatc ttctgttttt
     4801 gcttgttcag cttttttctc agcactcttc aagctgtgaa ttgatggatc accattttca
     4861 tccaccacac tgatacctgc gtcttcaatc ttttggatca atttgtccat ttcatctgca
     4921 ttcaaagtga atggtgtagc aagctgattg gataaatcat catatacaac ttgtcgtttg
     4981 ggtttgtttt ctttgatgaa agctgctact gctgcttcat acgttttttt atctgttcca
     5041 tttgccataa aagaaggccc cccttgagta gctatttggc ttgttttaac tgtttcgtta
     5101 aattgatgat ttcaatcgtc aattcaagct caagctgttt gtttccagtt cgtctggctt
     5161 cttgctgcat ttgttttttt aatgttattt catcagctaa gccagatttg ctgatcacat
     5221 ctagcaagtc ttggatttct cgtggcgaac tttcttctgc caccggtaaa tattcgattt
     5281 ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc taaaaactct gcaaggttga
     5341 attcataatg ttgtgagaca tatgcatcga ataaaaaata aacttctgca tatgcatcat
     5401 gaataaactg aaattctcgt tcttttaaaa tattacggac agaaacttct ttaaatgcac
     5461 gatataatag aatctgctct gccttttcta cttgcgtcaa aggtctcttt ttccgtgcag
     5521 tttcttgtct cgtaacctga gtgtgttgac tttcctgagg ttcttgacga ttttgctgat
     5581 ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc tctgccgatc tgaaactctg
     5641 ttgacagttg agatagatac atatcttgtt cgattggtga aacaactcgg ctaagttcaa
     5701 ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt atctaaattt ttcccttgtt
     5761 ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc atgagccact aattcttgga
     5821 aagatgcttc tccatactta cggagatatt catcaggatc aagcttttct ggaacgacta
     5881 cgacagaaag tgctaatcga ctgttttgcc gaagcaattc aatccctcga ttggttgctt
     5941 caaaacctgc gttgtctccg tcatagcaaa agaccaattc tttcgtcagc ttttccattt
     6001 gttggatctg ttgagctgtt agacttgtcc ccatagaagc taatccagtt ttgataccag
     6061 cctgccatgc agcaatcaca tccataaacc cttcgaacaa caaaatttct ccgtttttcc
     6121 ggatatcgct tcttgcttta tcaagattaa ataaaacttg tcttttattg aacagttctg
     6181 tttctggact attcaagtat ttgggctgat ctttttcctg ccctttttca ggtgcaagcc
     6241 agcgtcctga aaaaccgatc gtctggcctt gagaattacg aagtggaaac atcactcttt
     6301 gataaaaacg atcagacata cggccatcat catgcgtgac aaacaatcca gagcgttcta
     6361 acaattcatt tgaaattgat tcattcttga atacttgctg aagaaaagaa cgctcattag
     6421 gtgcaaaacc tatgttgaat tcttcaatta attctaaaga caacccacga ctttgcaaat
     6481 attctaaagc tggctgtcca gcttttgtat gcaatagcat gtgatgatag atttccgctg
     6541 ctttttcatg agcagcaatc agtttccccg tagcagattc aggattttgg acagctatag
     6601 tacgctgacg ccattgatct tctaacggta tctgttcgaa gtctgccact tttaccactg
     6661 cttctggaaa acttagacct tctagttctt gaagaaaagt aaacacgttt ccgcctttac
     6721 cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac agaaaaagac ggtgtacgtt
     6781 cttcatgaaa aggacacagc cccaagtaat ttttaccaga ctttttcaac tggacatatt
     6841 gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt ttcgataacc tcttgaggaa
     6901 tccgttgagc cattttttca ccccctgctc tattgatatc ttttgggttt aacagaagat
     6961 atttcaggag aataaccggt aaaaaatcgc actaactgaa atcagtgcga ttttaaagga
     7021 cacaattata catgtttgat tgtatcatat ttcattgctt tttcaagtat aacacatttc
     7081 tatctcatta agcatcaatt tgctactctt ttcagtaact gattttgaat atcagccttc
     7141 cagtacaata ctcgaagaac aatatttacg agcatgaata caaagaagta tcctacgctc
     7201 tctcccaacc ctaagtttac ttccaaaatt ccatggaaca gcattgcagc tacatatatc
     7261 cctacgatcg tcgttactgc taggaagatg cgcaatgggt tgaagggata acaagctttt
     7321 acaactgcca tgcaactgat cccaatcagc aagtaataca tcaaagtagt agtttcagca
     7381 tttccccatg cgaatgtttt tccaagtaga tacaccgcaa tgatattcac tactacaagc
     7441 aaggcatttg gtaaagcatt aataagtgct gtaggcaaat aacggtaagt gatttttctt
     7501 ttatcttgtt caaaagataa aaagaaggaa ggataccctt caatcgctaa atcaatcagc
     7561 gtgatttgga tagggataaa cggaaatcca attgctgtaa cggcacatat aatcgataaa
     7621 ataaatgaat aaatcgtttt gataaaaaag atgcctgaga ctttcgtcac attgttgacc
     7681 actcggcgtc cttcaaacag gacttctggc aatgtagtaa aatcagagtc caacaatacc
     7741 agattcgcaa tctgacgtgt tgctccatca ccttctgcca tagcaatgct gcaatctgct
     7801 tctcttaacg ccaaaacatc attcacaccg tcgcctgtca ttgcaaccgt tctgccgctt
     7861 tctttcaatt catttaccag caatttcttt tgctgcggac tcactcgtcc aaaaaccgta
     7921 tagcgatgag cagcttctcg cacttccatt tcttcagtaa tttgagaaag atcgacatac
     7981 gcatcataat tcggtaatcc tgcacgtcta gcgatattgg agacagtaaa aggattgtct
     8041 ccagaaatca ctttcagatc cacaccttcc tcatgaagat aagctagtgt ttcttgagcg
     8101 ttttttcgga ttggatcatc gatttcaaac agagcaaccg gctcaacaaa tgctggtttt
     8161 tcttcgttca attgctgatt tggagcgatc cccaacataa gtacgcgata gccattcgct
     8221 tgggctgttt ttagactgtc cggaagccga tcttcttcca ccaaacgttc aggcgctcct
     8281 acgtacacga ctcccagttc tggaaattcc atcgcgcccc atttgcgatc agaagaaaat
     8341 gcgactttgt ttgtcacatc ataccgatta gaaagctcaa aataatctct catagcccgc
     8401 atagtgatgt tgttatcact gctttctgcc agatacgtcc ccataatttc tgggatggct
     8461 ttctcgtaca gcggatgtag tgcttctacc cgctggatac tcattttccc ttcagtaatg
     8521 gtccctgttt tatccaagca taatgtatcg acatgggcca gcgtttcaat tgaatacatg
     8581 tcttgaacaa ggattcgttt ttttgccagt ttggttaccc ctgtcgttaa tgcaatactt
     8641 attagcaata ccaaaccctt tggaagcatg cctaacaaag ctgctgcaga tgcaataaca
     8701 gaaattttga tagaagcatc gcggatgaac agtgcttcca aaaatagtat gatccccaaa
     8761 ggaataatca cgaaactagt gaatttggat actttgcgaa tcgaatctac aagttcagaa
     8821 cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga cagcataatt atctgcacct
     8881 actcggataa cttcagccat gacagaaccg cttgtcaaaa agcttccaga aagcaattca
     8941 gctccagttt ctttgacaat caaatcagat tcgcctgtca aaagagcttc atttgcttct
     9001 gcccgtccag atataacctg taaatcggct ggtatctgtt ctcctgctcc gatttttacc
     9061 aaatctccta ataccagctc tttagtagaa atcgattgtt ctttaccgtc ccggatcact
     9121 agtacttctg attgggaaac aatcgataat ttccgtacaa gatttcttgc atgtatttct
     9181 tgataaattc caataacgat attgacgaca ataattgcta aatacgccat gttggaatac
     9241 gcacctacaa ataacagaca aatcccaata ccaaagttca gaaaattgaa taacgtcaat
     9301 atattatcgc tgaatatttg ttttgtactt tttgatgcat cttcctggta gtcattttgc
     9361 tggccagaag ccattttctg aactacctct tgctgtgtaa gtccattcat tttctttttc
     9421 tccattttcc gccaactttc aaaaataatc acgtgtttaa gtctagcgtg ttccaagatt
     9481 aaaaacaact tgtttttata aaatttatag aaaaacacac gaatgatttt acaaaaaaga
     9541 tgccaacctt tttgtttgaa tcacccgtgt gctgttttta ttatcaatct atctttactt
     9601 atattgttcc tccaaacggc tcatccacca acctagtgct gtaccggcta taaaaagaac
     9661 aggacaagct agtaccccag caactcccaa tcccgcgtaa tcggtaggaa tgatcataat
     9721 cgttgaagca aaaactacac ctaaaatgaa atggaatagc ttgctatatg cctttgagaa
     9781 aatgtaatcc atgatttttg acaatccaag aacacaaaga atcccaccaa ttccgatcgg
     9841 cacaatcact tcaagtcgta cttctttaaa tccgtcagcc atcgctttat acaatcccat
     9901 atacaccaaa aaattagaag ggctcaatcc tgggacgatc atacccaaac caatcaatcc
     9961 gccggcaatc agccatgttc caaaattctg atcgacttgc ccgtcaaata attgagcgcc
    10021 gtataataaa aacaaataag ccagaacaaa agtaacaacc atgatgatca catcagaagt
    10081 atctcttcct tttttccctg cttctttcca taacgcagga acagttccag cgatacatcc
    10141 gacaaaaaac cataatatcg ttgtttcata cgcacctaat aaaaaactga ctaaaaaaga
    10201 aagtagaaag atgcctgtca ctccgcctag accaacaggc agaaaaaaca aaacattttc
    10261 tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc cgctcataca tgccaaagac
    10321 agctgctaat gcaccaccgc tgactcctgg caagataaat cctgaaccga taaacattcc
    10381 cttcacaaac cgtaacatcc agtctttggt tgtgtacccc acttcttgat tttccatttt
    10441 ttgccttttt tccactattt gcgcactcct tctttcattt aattttattc atgattgaac
    10501 atttcctgct attttactta aagacttttt cataggtctc tattattttt tcattcattt
    10561 tgacaatatc aggataaaac atgttacctc cattatgata gagatagccg ccattgtaaa
    10621 gcaaggactg gatccgccaa tatcggtatt gtttcccttc ctcatttcct aaaagtgggc
    10681 tcagcacatc tttagaatac gcttctgcta attctactgt gttttctccg ccatgatttt
    10741 ttacataggc aatataatct ttcccaaaat tatatgcttg gacagctgtc cataaatcgc
    10801 atccttgttt ctttgcttct actatcattt ccgccaaata tttgacacct tgatcaatac
    10861 tgtcagctgg attatcaatg atctcagctt ctccatgcag actttccgag ctctgcatga
    10921 gatctgtccc ttttcctttt gattcggtca atattatcgc cttgatcaaa ttctcgtagt
    10981 tttccatatg ataatttttt gcagctgttg ttatttctat ttgatagctt tccactcgtt
    11041 ttaccgaacg ataagttctt cctcataaaa acaacaccgc accaataacc gtcagaatga
    11101 tcatgtaaaa aagaatacgg ataatagata atcgttttcg cttcatttca tcacctagtt
    11161 attttgacat ctttttttat cttactattt ctttatcttc ttggctattt cttccttgat
    11221 ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt ttttgacatc ttacgaaaat
    11281 gatcgttgtt tttcagtaaa aacaattgcc caatgactga tataatcgtt atactttaca
    11341 cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa aatgccagat gggagcgaaa
    11401 tttactatga aaagagcggt caaggcttcc ctctcttttt actgcatggc aatgacggca
    11461 gcggccgctt tttttcagaa caggtccctg tactcgaacg ctactatacc gtgtatctag
    11521 tagatagccg aggacatggt cgttcgacaa acgaagcatc tatgttgaat tttcagttaa
    11581 tggccgagga tctgaatacg atcatgttgc tggaaaaaat cgatcaggca gattttcttg
    11641 gatttagtga tggagcaaat ctagcactgg tatttgccag cagcttccct aaaaaagttc
    11701 atcgattgat tttaaattct ggaaacacgc ttgttaaagg cgttcgtttt tctgccagag
    11761 tcatcagtaa tattcattat gcatgggtat ggttactctc tttatttcgc ccaagtcttc
    11821 gcaaaaatct tttagttatc aaacttttgc tgcatgacat cgggcttaca gaaaatgatt
    11881 taaaaaaaat caactccccc actttgatca tcgtgggaaa aaaagatgta atcaagctaa
    11941 aacattcttt gtatatagct aaaactatac caaaagcttc ctttgtgctt gtgaaagaac
    12001 aaggacatga attagctaga aaagatcctg aacgcttcaa tcgagaagtt cttcaatttc
    12061 tttctgaaac ataactatta ggaatagaag gtgatttatt tgttaaaaaa taccatacaa
    12121 tggctgaaaa accacctagg actgttcaaa acaatctttt tgatttctgt cattgtcatt
    12181 attgtcggtg aattgatgtc catcggtaaa actctatcta tccagcaatt aggagaaacg
    12241 tttgccacta tacctgtatg gaaaagcgga ctaatgctat tgattggtct tatctctgtg
    12301 ttacctatgg ttggttatga tattattttg aatcggatgc ttgaacaaaa gcagaatcct
    12361 cgttacctgt ttgagacaag ctggctgatc aatacgatca acaatattgc cggtttcggc
    12421 gggtttgtta gtgtggggct tcgctctgaa ctatatggac aaaagaaaga gagcagaaac
    12481 gtgatccaag cattatcgaa agttttcttg tttttgatgg ctggactttc tatttacagt
    12541 ctgctttctt ttttacttgt agtcttcacg ccagttgctc cttttttgaa gcaatattgg
    12601 atatggttga tcggcggcgg tctatatttt cctgtcgtct ttttatttac tactttcaaa
    12661 aagaaaggat tccttggtgg tacctcactt aaaactcaag gacagctatt gctagtttca
    12721 ttgttggaat ggaccggtgt cctgtttagt ttcctttctg ttggttattt aatggaaatc
    12781 cggattgatc tgtggcaaac aattccatta ttcattgctg cttcagtgat aggaatcgtg
    12841 tctatgattc caggagaaat tgggagtttc gatgtaatga tgattatcgg gttgtccgct
    12901 attggtgtgc caagagaaat agtcgttgtg tggatcttgc tctatcgcct gttctactac
    12961 atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa atatcggttc cacttttgat
    13021 cagcgttatt ctggtatccc taaacaatta gcaacagaaa ttgcgcataa aatcgtggtt
    13081 gtcttattat acttttctgg aatcatgcta gtactttctg ccactatccc gcaagctttt
    13141 acagagtttc gctggcttca tagtttgaac ccattgaaat ttcattttat tatccaattt
    13201 cctagtatac tattgggatt tcttttaatc attatgggca gagggatcgc agcacgtgtg
    13261 aaacgagcat atttgcctac aatcttcttg attgttctag ctttacttta tgttttatta
    13321 agtgatttta gtttcacacc ggtggttttc ttgtctattc tgcttctgat cattcttgct
    13381 tctaaaaacg aattatttcg ggaacaattg atctattcat gggagtggcg tacgatcgat
    13441 ggaatcatta ttggtgcact gtccttgctt tacatcgtta ttggggtata caatctgccg
    13501 gatttccctc atcggcgtca ccattttatt tcctttttct tatttccttc tgaaaagatt
    13561 tggttttcgg gattgttggc catcatcgct gttagtttca tgatcgttct atttgttcat
    13621 ttccttcaag gagaaaagaa acagattggc gaagccttta atgaagaaaa ggccctgaag
    13681 atccttacaa cttatggagg aaactccgat agtcaattga tcttcttaaa agataagcgg
    13741 atgtttgcct atgaaaaaga cggtgaacca actgttcttc tccaattcgc ttgcttcaat
    13801 aataaatgta ttgtaatggg tgatccttct ggtaagaaag aagattttcc agaagcaatc
    13861 gaagctttta tcgaagaaac agatcgacta tgctatttac cagttttcta cgaaacaagc
    13921 gaagagattg ttatgatttt gcatgaattc ggctacgatt tcattaagat gggggaagaa
    13981 gcttatgtag acctgaatag ttttacaact tcagggaaaa aaatgaaggg cactcgtgct
    14041 gtcttgaatc ggatcgaacg agaaggattt acatttgatg tcttacaacc accattttct
    14101 actgagcaaa tgagtacttt caaaaatatc tctgataatt ggttgggttc acgaaaggaa
    14161 aaagggtttt ctttaggctt tttctctgaa gattatctac aaagagcgcc tattgcagtt
    14221 gtcaaaaata cccaagaaga aattgtcgct tttgcaacga tcatgcctac ttacacgaac
    14281 aataaagtag gaacgattga tctgatgcga tatgattcta aaaaagcacc ttctggcagt
    14341 atggatttct tgttcttgca tttgtttgcc tatatgaaag aagaacatat ccaatggttc
    14401 aatttaggga tggcccctct ttcaaatgtc ggaacttcca gaaagagttt tttacaggaa
    14461 aggattgcat acttggttta cgagtttggt tctcattttt attctttcca tggattaaga
    14521 gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt atactctata ttctagagat
    14581 agttggattg catatgttat gattgctttg ctgattattg ataatgcgcc agttgagcag
    14641 acacaaggta gaataagtgg tttgaaaaaa tggatacgaa gaaagtatta aaaactgcat
    14701 accgatagat gaacactctc atgatgaata aaagagactg aagtaaaacg aaagttcatg
    14761 cttctacaat ctcttcttat acaattgaag aacaaagagc ggtccaaaca gaaatcctgt
    14821 ttggaccgct cttttatttt aaacattttt cattaccaaa atattcgagt cgcgtttctt
    14881 atgcttcgat ctctgtaacg atacctgaac ctactgtccg tccgccttca cgaattgaga
    14941 atgtcgttcc gttttcaaca gcgattggat ggatcaagtc aacatcaatc gtcacgttgt
    15001 caccaggcat gaccatttct gtatcttctg gcaatgtgat tgttcctgta acatcagttg
    15061 tgcggaagta gaactgtgga cgatagttgt tgaagaatgg agtatgacgt ccaccttctt
    15121 cttttgtcaa cacgtacact tctgctttga attttgtatg tggagtaatg gaacctggtt
    15181 tagcaagtac ttgtccacgt tcgatatcgt cacgttggat cccccgtaac aacacgccta
    15241 cgttatcccc agcttccccg taatctaacg ttttacggaa catttctaca cctgttacaa
    15301 ctgctttttg tgtttcaggt ttgatcccta cgatctctac ctcatcaccg acacgaacag
    15361 caccacgatc aatacgacct gaggcaacag ttcctcgacc tgtaattgag aagacgtctt
    15421 ccactggcaa tagtaatggt ttatctgtat cacgttctgg tgttgggata tattcatcta
    15481 ctgtatccat caattccata atagcagctt cagcatctgg atcgccttgc aatgctttta
    15541 atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa accatattcg ctcaataact
    15601 cgcgaacttc catttctacc aaatcgatca attcttcatc atcgactaaa tcaactttgt
    15661 tcaagaaaac aatcaggtat tttacaccaa cttggcgtga caataaaatg tgttcacgtg
    15721 tttgaggcat cggaccgtca gttgcagaaa caactaagat tgcaccatcc atttgtgctg
    15781 cccctgtaat catgtttttc acatagtccg cgtgtcctgg tgcatcaata tgcgcgtagt
    15841 ggcgtttttc tgtttcatat tctacgtgtg cggtattgat cgtaatccca cgttcacgtt
    15901 cttctggagc tgcatcaata ctagcatagt cttgaggatt tgctaggcct tttttgccta
    15961 atactgtagt gatcgcagca gttaaagtag ttttcccatg gtcaacgtgt ccaatcgtac
    16021 caatgttaac gtgtggttta cttctgtcat aatgttcttt tgccataatt tacaaacctc
    16081 ctaaataata tagatcactg aaaaatttca gctctcacct ttttattata gtcttttttt
    16141 gactaaatca aaaagaatac gtccgactta ctttttgtaa gattcgaact aagcttatta
    16201 tacgtcaata ttggtcaaag tcaactaaga gcactcgtct gtcttatacg cgaaaaaagt
    16261 accttttata aataaaaaaa tgtgtttttt gagaaaaact tcaagatagt ccttaacttt
    16321 cttttgaagc aataagaaca aaatgaggaa aagtttaaaa ttcagctgtt gccaacaact
    16381 ttcaatagac gaaatcttct atttcgttaa taataaggaa agaggtgaag tacatgaaaa
    16441 aattaattgg cgtacttgtc gttttaggca tatttctcgg cggaagtttt gcagcttatc
    16501 attattttta cggcggcgaa gcttattata caaaaatcac gacgaatggc gaaatgtcag
    16561 ctacccaagc agataacgga gagaaattca cgacttatac ctacaagcaa gcggcttata
    16621 ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga aagagaacat ccattaaaga
    16681 tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg tgtattaagc tgggaagaaa
    16741 taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat cgataattag taaatggtct
    16801 taaaatttta ttagataaaa agaggctggg acagaagcgt ttaactccaa gaaataagaa
    16861 ggagttgctt ctgcttccgc cgtttatacg tgtttagagc gtgagacaaa agtgttcttt
    16921 acttttgtct cacgctcttt ttttgttata ttcgtctttt ccgatccttc tcttgacaca
    16981 attcatttaa ctgatcactg gtacttgaac acctaatatt ctagtcattt ttataaacga
    17041 ttcaatactt catccacatt tttgatcatc acggatatcg taccatccgg attattcaca
    17101 aattcaatca aatctgggtt gcgatataca tctactggta cgatcagttc gatcccgttt
    17161 gatagcttga atttttgttt tccaaatttt ttctcagaaa tttcttgtac ttcttttaca
    17221 ggaggtgctt ctgggacaaa tccttgctct tgtacttctt ctttaaaagc catttttgcc
    17281 gtgatgtttt ccttgaagac tttttccgca atcatctcat gatccaaccg acctttttct
    17341 tctatcgtat catagacagc ttctttcaaa tcagccatga tatcaaattc ttctgtctca
    17401 aatttttttc caagatgctt ggctactttt tggatcactt ttacattttc ttctaaagaa
    17461 ggtgcaggaa cactttcgat cactcttcca gaaaaataaa gctctttttt gccagaaaac
    17521 tcatattttt tttcaattaa ttcgtaggtc caatcagtca aattcacagc gaatgcttca
    17581 tctgcttttt gtgttttcgc ccctagaatt gctcgattca aaatcaagcg gttgtcgatc
    17641 cctgcttcgt ctgcttcaac aaagtgagtg taagcctcac gataattgac tttcaataaa
    17701 gataagtaca tcactgtatc taattcatac aagacaacaa atacatctgc actaggtgct
    17761 tcttcacttt cttggtagat atcgaaccac cgctgaacga ttgcttcact tgcttccaca
    17821 aaattttctt gagcgatttt aagtgattca acgatcatgc tttcttctgc tagttgtcca
    17881 gtttttgttt gagcagaaga gattttttgg atcttctttt gaagaaaatc acgaatatat
    17941 tcttttgtca gatccagctc tttttgtgaa aagactggag aaccagtttc tctgtcaata
    18001 atatgcagaa ttgctttttt taaatagata tccattttct cattcccttc tgtcttagtt
    18061 tacggaaaaa aaatcaaaaa taccagaatc aaaatcattt tcttttaaac aaaaatttca
    18121 agagtagaaa caaaatggat tctttttttc gaaaaatgcg ctacacttat agttgaacga
    18181 ataaaaaagg agctgaaagt atgaattcat taacaagcac ttatcgtcta tcaaatggtt
    18241 acgaaatccc ggtagtagga ttcggcacat ggcaaacacc tgatggggat gtcgctgttt
    18301 cctctgttaa agaagcgctg gctgcaggtt atcgccatat cgatactgcc caaggataca
    18361 agaatgaaga aagcgtcggc caagcgatta aggaaagtgg aattcctaga gaagagatat
    18421 tcttgacaac aaaattatgg aatgcaaacc acagctatga acttgtgatg tcttcttttg
    18481 aagaatcatt gaagaagctc caaacagatt atttagatct gtttttgatc cactggccaa
    18541 accctgttgc ttttcgtgat aattgggaac aagcaaatgc agatacatgg cgagcatttg
    18601 aagaactgta tgaagcaggt aaaatcaaag caatcggtgt gagcaatttc ttaccccacc
    18661 atctagatac actagcaaaa acagcaaaaa ttatgccgat ggtcaatcaa gttttccttg
    18721 caccaggcga attacagaca actgtagttg agtatgcaaa aaaacatgat atgatcctcg
    18781 aagcatatag tccattaggc actggtaaaa tcttcgatgt gcctgaaatg aaacaaatcg
    18841 ctgaagcaca tgacaagacg attgcacaag ttgctttgcg ttggtcgctg caacacgaat
    18901 tccttcctct tcctaagtca gtgacaccaa gcagaatcaa agaaaatact gaattatttg
    18961 attttgaatt aacagaagaa gaaatgaaac aaatcgatca gttagatggt gtagtcggca
    19021 aagcaaaaga cccagatact acccaatttt aagtcgaaag gtgattttat ggatccaatc
    19081 gaacgcttga attctctctc ggaagaagtt acccagactt ttcattccga ttttgtcttt
    19141 ttgatcgatg cagagaaaat ccaacatttt cctgcaagaa actggacaca tgatcaaatc
    19201 atcgaagaac tgaaaaaacg gttcgatcat tcgctaatgg tcaaaccctg gcacgaacat
    19261 gaagtcattt attcacctga actacctgtt tttgccttga taccgaaaaa atagaaagaa
    19321 tggaacggat tatgtatata tacataatcc gttccatttt ttatttttct ttcaaaaaac
    19381 gggaaaatac gcaaattttt ctgttgtaat ttgcgtatac gcaagttttt ttgcattact
    19441 ccttttcatt tggctgtttt tttcttataa tcgaaagggt atgagcgaag gagagaacat
    19501 atgaagaaaa aacagaaaca aaaattatta aatcaattcc gtccttcatt ggatggtgta
    19561 agaggacaat tattccgttc tctagaagaa gagagcttgc gacgttatgg tttgccgtta
    19621 caagtcatgc ttgatcccaa aaaccgtcaa atcttaacga tcggattagc tgggaaaaca
    19681 aatgaagata cacggatcaa cgtgccaatc gatgacaact tcaatactgt attaaagaga
    19741 attcgttcag gagaaaacgg gattttcgaa cgattccgtg acaatcttct tattgaaatc
    19801 gtgtcttact ggaatgatca acgattgaaa aacgatgaac caactgcgca agctgttgcg
    19861 acaccagtta cagaagaaac agtcagtgtg ccagaagaaa aaacggaaaa accaaaagcg
    19921 gacacaactg aaaatacacg ctcaactgat acgaatgccg ctttaagttt tgctgatttt
    19981 tcaaaagccg ttgctgagta tcctaaattt tacacagaac aaaatgaaca tgaagtagtt
    20041 attaatgaaa aaaatgctga tgaacctcgt aaattagcaa ctatttctat gactactgaa
    20101 aatgagttcg ttatcgaaaa agcactcgaa cggaaataca aagtcaaatt gactttgatt
    20161 cctttgatcg aacaatttgc agcgacagcg attactgatc gataaaaatt ctatttgtca
    20221 taaaaggctg ggatttaaaa tcccagcctt tttagtcata taataatttc tacagcaagt
    20281 aaaaagaaat tccttctaac atcaggctca aatatcagct tgacctgt
//
LOCUS       DACDSY010000040        20069 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDSY010000040 DACDSY010000000
VERSION     DACDSY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.040"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..470)
                     /locus_tag="IWK44_001198"
     CDS             complement(<1..470)
                     /locus_tag="IWK44_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9549735.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKA"
     gene            complement(559..1740)
                     /locus_tag="IWK44_001199"
     CDS             complement(559..1740)
                     /locus_tag="IWK44_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP9549736.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(1976..3484)
                     /locus_tag="IWK44_001200"
     CDS             complement(1976..3484)
                     /locus_tag="IWK44_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP9549737.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            3643..3834
                     /locus_tag="IWK44_001201"
     CDS             3643..3834
                     /locus_tag="IWK44_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549738.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            4037..4954
                     /locus_tag="IWK44_001202"
     CDS             4037..4954
                     /locus_tag="IWK44_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP9549739.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            4947..6020
                     /locus_tag="IWK44_001203"
     CDS             4947..6020
                     /locus_tag="IWK44_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549740.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            6168..6686
                     /locus_tag="IWK44_001204"
     CDS             6168..6686
                     /locus_tag="IWK44_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549741.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            6667..7059
                     /locus_tag="IWK44_001205"
     CDS             6667..7059
                     /locus_tag="IWK44_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP9549742.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            7135..8070
                     /locus_tag="IWK44_001206"
     CDS             7135..8070
                     /locus_tag="IWK44_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP9549743.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            8203..8982
                     /locus_tag="IWK44_001207"
     CDS             8203..8982
                     /locus_tag="IWK44_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP9549744.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            8975..9292
                     /locus_tag="IWK44_001208"
     CDS             8975..9292
                     /locus_tag="IWK44_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9549745.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            9536..11146
                     /locus_tag="IWK44_001209"
     CDS             9536..11146
                     /locus_tag="IWK44_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9549746.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            11303..12151
                     /locus_tag="IWK44_001210"
     CDS             11303..12151
                     /locus_tag="IWK44_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9549747.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            12382..12861
                     /locus_tag="IWK44_001211"
     CDS             12382..12861
                     /locus_tag="IWK44_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549748.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            12894..15122
                     /locus_tag="IWK44_001212"
     CDS             12894..15122
                     /locus_tag="IWK44_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9549749.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            15251..16894
                     /locus_tag="IWK44_001213"
     CDS             15251..16894
                     /locus_tag="IWK44_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9549750.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(16967..17512)
                     /locus_tag="IWK44_001214"
     CDS             complement(16967..17512)
                     /locus_tag="IWK44_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP9549751.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(17535..18020)
                     /locus_tag="IWK44_001215"
     CDS             complement(17535..18020)
                     /locus_tag="IWK44_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9549752.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(18010..18822)
                     /locus_tag="IWK44_001216"
     CDS             complement(18010..18822)
                     /locus_tag="IWK44_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP9549753.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(18961..>20069)
                     /locus_tag="IWK44_001217"
     CDS             complement(18961..>20069)
                     /locus_tag="IWK44_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9549754.1"
                     /translation="ILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAHS
                     MGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKVV
                     HMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTSM
                     AQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLPE
                     NTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLIE
                     TINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELPR
                     LNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         6556 a         3680 c         3943 g         5890 t
ORIGIN      
        1 atagctttca aacgtttcaa ctgtgctaaa tcttctacat aaaccgccag tctggctgaa
       61 atctgttcag atgaatctcc acgttttttc aggcgcgatt gcaatgtttc tttagagatc
      121 gtaataaaga taggacggac taccgtcccg aaatactcat ataaatgcca aaaaccattt
      181 gctgtcaaaa cggcgtaaca atttcctact gtcagtttct ttttaatttc tgctttactg
      241 gaaccatatt tatgttgagc atattggtcc cactctgcaa actcatttcc cttgatcatc
      301 tgattgaatg tattgtcaga aacaaaataa taatcctctt gatctttttc cccaggtctt
      361 ggtgatcgat tcgtgtaagt aatgatcttt tgttccggct gaaaaatagc ttctgcgagt
      421 gaagtttttc cagaaccaga aggaccgata aatacatagc aataattcat tgtcatttct
      481 cctttgatga ttgaacaagt gaaaaagctt tggaaaatat ttccaaagct tttaaaagta
      541 actttgcttt tttgtatatt ataccagcaa gccagtcaca atagcactta aaaagctgac
      601 taaagtcgct gttagcaaaa ttttcaatcc gtgttttgcg acaagatttc ctttttcttc
      661 atttaatccc ttcatcgcac cagaaatgat cccgatagaa gagaagttgg caaatgaaac
      721 gaggaagaca gatataattg ctgttgtacg ctcagtaaaa tggaaattac catttgctaa
      781 ttctgtcata gcaacaaatt catttgtaac cagtttagtc gccatgatac taccggcatc
      841 cactgcttct ttccatggga ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag
      901 gatctcttgg aaagtgatgc cgaatatccc gtggaagata gcattgatca tggcaatcaa
      961 agcaacaaaa ccgataagca tagcagcaac cgtgatcgcc acatggaaac catcaagaat
     1021 gtattctccc aacatttgga aaaatgtctg ttctttgttt tcttcaatga tcagctcatc
     1081 ttctttttct tctaatacat agggatttac aattgaagcc agaatataac cgccaaataa
     1141 gttcaaaaca agggcagtga tcacatattt agaatcgatc agtgccatat aagatccgac
     1201 gatcgacata gaaactgtag acatagccga tacactcatg gtgaacaaac gtttctcaga
     1261 taggaagggt aattcctttt ttatggaaat aaaaacttcc gattgtccta aaatagcaga
     1321 agctactcca ttataagatt ccaatttgcc catcccgttg attttactta atcctagacc
     1381 aacagctttc ataaataatg gaagaatctt gatatagcgt agaataccga taatagcaga
     1441 aataaataca attggcatca atacggaaag aaagaaaggt gcacttcctt tatttgctac
     1501 accggcaaaa acaaagtcaa caccttgccc agcaaaagca agaagatgat caaacatttt
     1561 tgccaataca gaaacaaccg ctgtaccaaa agttgtacgt aataatacga aaccaagaac
     1621 aagctgtaaa acgaacatca caattagtgg tttcaatttg attgcttttt tgttactgct
     1681 gatcaaaaaa gcaagcccta atgtgacaaa taaaccaata atacctaata aataactcaa
     1741 ataatcactc ctattccaaa ttccgtttta ttttacctgt tttgcaaata aaatactaat
     1801 ctttagtgaa attttcatta cttttttaga gtgaaacgca ttcattttcc attagaaaat
     1861 aaagcgaaca aaataaaagg tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac
     1921 gaaactatcg attaagctaa tagtttcgtt ttcaacattt ataagtaaac tttttctatt
     1981 ggtaatatag attttcggaa gggtagacag gtgcgcaggt aatatcgatt cctagttttt
     2041 tcaatgtttg ttcatcattt ttattgacca tcacagtaga gtgggcttgt gctccctcaa
     2101 gttcagctag tttatcataa gcaacttgag cgattggatt cgttactgca ctgattgata
     2161 aagcgatcaa gacttcattg atgcttagtg catccaaaga ggaatgaagt gtctttgatt
     2221 tcaatccttg tattgtttca agaactaatg gtgacaataa aggaatgtca tctgcgatat
     2281 gggctaaatg ttttactgca tttaagataa ctgccgctga cgcatccatc aatgaacttg
     2341 tacgcccggt aatgatgact tcatctggta attcgattgc aatcacagca gaaggatctt
     2401 ttgtaggact ttgcgcttga atcttatctg catagtcatg ggcagggact accacgtttc
     2461 ggtcttcagg acgtaaaccg acttcttcca taatcagttt gattcgatta actgcttctt
     2521 cgtcgatcaa tccttttttg aagtcgcatt ctgtttggaa ataacgacgg atgatttctt
     2581 gtttagaggc ttcttggaca acggtatcat ccacgatccc aaatcctaca cgattcacac
     2641 ccatgtcagt aggagattga taaacagatt ctttaccagt aattttttca ataatacgtt
     2701 tgattacagg aaatgtctca acatcccgat tatagttcac agctacttta ttgtaggcat
     2761 caaagtggaa ggaatcgatc atattgacat cttttagatc gactgtggct gcttcataag
     2821 caatgttcag cggatgtttc aacggaacat tccatactgg gaaagtttcg aacttggagt
     2881 aaccagctgc atgtcctttt tggctttcat gatacagttg gttcaaggaa gtagcgagct
     2941 ttccacttcc tgggccagga gctgtcacta caacgattgg tttagttgtt tcaatatact
     3001 cattttttcc aaaaccatct tctgagacga ttttttctac gttgactgga tagtcttcga
     3061 tttctgagtg tttatagacc ttgatgtttc gtctttctag tttattgata aagactttcg
     3121 tggcaggctg tccactatag cgagtgataa cgacactgtt gattaataag ccataaccac
     3181 gtaattcatc gattaaccgc aagatatcca tatcataagt aatcccataa tcaccacgga
     3241 tcttattacg ttcaatatct ccagcataga cgcaaatcag tatttcagct tgatctttca
     3301 atttttgcag caatttgatt ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat
     3361 gcttgtcgtc aactagcttc ccaccaaatt ctaaatataa tttgtcatag tggttcactc
     3421 gttccaaaat atacgctgac tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt
     3481 tcactaggtt ccctccttaa gtactaaagc attttttcat ttttttgagg attcgtcaac
     3541 tggaagagcg aaattcctca aaaaaacaaa gaaaatggtt taaaccaact tgtctagata
     3601 gttattcatt ggtatgatag tttttgataa ggaaggtgct acatgaaaaa aagacagaaa
     3661 aagaaaaacg catataaaca ctatatccgt tcgattttta ccggttatga aagaatgctt
     3721 gaagatcccg aattggaaca attgacgttt acttatctaa acgaagaaac gcagctaact
     3781 cgtgatgagc atcagcgcat tcatttcaca acaagagatc tgcctagtaa ataaaaaata
     3841 agataaaggt catcggattc gattctgtcc tatcagatgg aaagactagt tgatcgaaaa
     3901 aaaggctgta ccaaattttg gtgcagcctg ttttatggag cattgcccgc tcttttttat
     3961 aacaattttt tacagaaaat ggtactattg aagtattaat aagtgaaaaa cagatgagta
     4021 agaggaggca agtagtatga aagagccaga tacaaatgca aattatgagc cagacgaact
     4081 agaggagacg aatgcggatc aggatacagg acaagaatcg tcttcttttt acgatatcaa
     4141 agaaaaagca agcatgaatc tttcccaatt attagaagac ttacaggctt ttgaaaaagc
     4201 tgtccgcgaa gaagatatcc ctgagatcta ccgtttatac aatggccgat tgaatgaaga
     4261 attaaaaaga agctcaaatg aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca
     4321 tgatcgtttt ttacaggaat ttccttttat ggaacaagta gaaaacatct cgccaacgat
     4381 gagctattac aaaatcggca cttattacca tgatcggccc acggtcggta ttgatgcaag
     4441 tctgccagaa atttttgttt tgcctaagat cgatgaagag tgggagaaat attcccaacc
     4501 ggagtcaacg gactatgaca aaaaaatcaa tgagctagac gcaaaggtga tcactgctca
     4561 gacagaaatt gaacgtttag atgaacaaat aaaagaaatc aatcgacaaa tgactgattt
     4621 gaacgacaat aaaggattct tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga
     4681 gaagaagaag caagtactat ctaatgaaaa attaggctgg cttccttata ttgagacgcc
     4741 tgaaacaatc cagcagcaaa aagaagcatt gaagcaagaa gcaagagcag accagcttcg
     4801 tgcagccatc gtagaaaaag aacaaaggca aatcagacgc tatttcggta gcaaagaggg
     4861 attcggtcaa gcaatccatg aattcttgat gaattatctt ggcaatgaga atcctaataa
     4921 tcaatctaat gaaggaggtt ccgagtatga atgaacacga tgaagcagaa tggacaaaaa
     4981 atgaagaaca aacagaagat catgaagaaa cgaacacttc attggaagaa caaacaagta
     5041 atcctctgga tgacacagaa gaaatcccta tgctagagga aacaaaaata cacgaaaaag
     5101 aaaaagtaac tccgacgaat acgaatactg cagaaaacac ccatgcttac gaaataactg
     5161 aaaatcccgg gtatttagaa ggaaaagagc aagatcatca agatatcgtc agtttgattg
     5221 aaacagcgga agcagaatta gcggatttac gtatgagaaa agatttgatc gaatcagatt
     5281 ttccacaatt attagaatca tatgatcgtt ttatattgaa tgaagaacca atctccacga
     5341 tcggaaagac aagcctgatc gaatacttca cgtatgaatt actgaaaccg ttgaatgata
     5401 ttggtctgga acggtcaaat tcagcttatg acacatggaa aacaagacat ttttcgatca
     5461 attatacgtt gaatggacaa aacgaattag tcttttcctt tgtattaccg accatcgatc
     5521 aacgatacca agtggaatcg atgagtctcc taaacgtatc accagagaca atggaaatca
     5581 atgtccaaga tgaccgggtg ctttctttga tccgacactg gtcagtggat cgagtattct
     5641 ctgcagggca gatcacgata ttcaatcaca aattgaatca attattagcc catgcaagaa
     5701 cacttggttt taccgttaat cagacattac tagacaatac gaagccactt cgtttgaatc
     5761 ttcagagcga atttgaattg acgaatgaag tattggatga tatttttatc gtggcaatga
     5821 aaaatccatc ctacgatatg gaaaagatca acgaaactac ttatcaagta cgattagata
     5881 aaggacagtc tttaacgata acaacaaacg aaaaagatca aacagtcttg tcgatctcat
     5941 ctggagacta cccacgatct gtcattgatt tctttatcaa ttacgaattc ttggtcccat
     6001 taatggtgag aaagtcctga ttggataagt tggattacca gatgtcattc agataattct
     6061 gttccaaagg acttgcggca gttatccaga ataattgtca caagtctttt ctgtatgtac
     6121 taaatggacg tgaataactt gctgatgaag gagggatttc attaggaatg tccacaaata
     6181 aaatagcaca gcttagctta ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat
     6241 ttattccgaa tttccagcca atgactgcta tttttttatt tatagtactt tacttaagct
     6301 taaaagatgc gttaattgtc atgagtcttt ctattgcgat cagcagtttt tattttggtg
     6361 ttggaccatg ggtcatcggc caatggatca gctatactgt tgttctgagt ttgttttcaa
     6421 tcgtctgctt gagaaaaaca gtgcaaagga atctatggtt aaagggagtc tgtttttttc
     6481 tggcgggcat ggtttacgga ataggtatga ccgtttttga tacgctgtta tatcgtttgc
     6541 ctcaaccttg gatctattac cttcaagggg tgtcgtttga tttgatgcac agtataggca
     6601 atgttgtgtt ctttcttgtt tttctgccag tcgtcaaacg tttttacaat cattcaggag
     6661 gaaaaaatga aaaaaataat ttatagtttg cttgcaatga gtgtcagtat actcattttg
     6721 acaagttgca gtacaaccta tacacaatcg gcttccaacc aaaaggagga agtaaaaaca
     6781 tcgcaaaaag caacaatcac gttacaagaa aatggtcaaa acttttcgga aaaagaagtg
     6841 ttcttcaaaa aaggcgatga tttactgagt attttgaaac gaaattttga agtcaaagaa
     6901 gataacggct tcatcacttc gcttgaaggg cactcacagg atgagaaaaa tcaactctat
     6961 tggatgttta ctgtggacgg gaaaagtgct actactggtg ccaaagagat caaattgaat
     7021 gatggacaat cggtcatttt caatttgtct aaactataag tttcataaaa aactgttaaa
     7081 gggcgaatat tgccataatt cagtgagttc agttgatgaa agaaggaaaa taacatggaa
     7141 aattttaaaa aaagcggaat gttttccgta attgctgctc taggggctaa tattctagtt
     7201 gctatcagca aatttgtcgg ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc
     7261 atccacagta tcgttgattg cagcaatcag attttgctgt tattcggtga taaacaagcc
     7321 aataaaggac agagcgagtt gcatcaattt ggtgaaggac gagcaaaata ctttttcagt
     7381 acaatcgtcg caatgatgct tttcttcggc ggtggggcac ttggggtgat ggaagctttt
     7441 gaaaagttga cgcatccatc ccatgaagtg ggtaatccga tcattgttat agcaatactg
     7501 ttatttggtt tggtaatcga aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa
     7561 ttgaatactg aaaaactttc cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata
     7621 ctgattattt ttacggaaga cttgtgtgct gtaatcggtt tggtcttggc tttagcagga
     7681 acaatattga cttatgcaac cgggattgct gcatttgatg cgatcagcgg attgctgatc
     7741 ggtttcttgc tgatgggagc cgccattttc ctagcaaaag aattttatag tctgatcatc
     7801 ggtgaaagcg tgacagcaag cgatttatca aaaatcaagt tagcttttga acgtcctgag
     7861 atcagtcggc tgatcgatat aaaaacagtt catttaagtc caacggagat tttagttgca
     7921 gcaaaagcag atatcgttgg taacaaagag aatgaagctt actcggtcat caatgatatt
     7981 gaaaaaaata tgcgtcagtc tcttcctgac aagaagatgt atatatacat tgaaacagat
     8041 aaatatgacc caaattattc tcgtaaataa aacgtttatt catgaattga agcaaaaatg
     8101 agaaaaatat aatcttgttt ttaaataaaa cggctacaat ttagcgaatt tatgttacaa
     8161 tgagtccgct gtttatttgt tgtaattaaa ggagatattt taatgaacaa aaagaaaagt
     8221 gcgccatggc ttcaagcttt gcatgcagtg atgccacttt gtataagcta tataccagta
     8281 ggattagctt gcggagtatt gttgcaaaaa gttggatttg atcctctctt atcaggactg
     8341 ttatcgattc tggtcttttc cggtggcgcg cagtttttag ttgcatctat gcttacgacg
     8401 caagcatcat ttgcaacgac attattgatg gttttctttt tagaattacg ctatacatta
     8461 ttaggagcaa gtcttgccgg ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt
     8521 tctcaatcac tgaatgatga aaactatgcg gtgaactact taaagttttc aactgaccct
     8581 tcctggaata aaagaaaggc tttatatgtc aattggttct caatgactgc atgggccggt
     8641 agcaatatga tcggtaattt tttcggctcg gtgattcgag tagatgctga ccttgtccat
     8701 tttgctttga cggccatgtt cattttcatg tttatcatgc agatgaaaag cgcactactg
     8761 atctttacag gtttattctc aggcgtatta ggggtcgtat tcatggtatt attccaaaat
     8821 actttcggat tgattgcagc aacagtcatt gcttcatttg ccggatttat gctggagaag
     8881 gcattcaaaa aagaaaaagc taagaaacta agagaccgcg gtaaagatgt caatgagccg
     8941 ctcttcaact tcccggaaca ggaggccaat caccatgact aaactatatt tgctactttt
     9001 agttgcttgt ctgttcatcg tggcatatat cccgcgcttg ttcccgatgc tttattttac
     9061 tcatcgaaaa gtgccatcct ggttcagtga ctggatgaaa tatgtaccgg tggcattatt
     9121 tgcggcgtta gcttttaaag atgttttcat tactcatgaa catctggata ttgcttggaa
     9181 tatcaaaata gctgcaatga ttctagtagc aggcgtggct tacaaaacac gttcgatggc
     9241 gttgtcagtt ttgacaggac ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt
     9301 attttattaa aaacagagat tgcgacataa gttgttcaaa ctgatctatc cgaactgcat
     9361 ttgcaacagt caattctgga tgcaagttta ttcgtaggga taaaaaagat tagtgttggc
     9421 ttatgatgca agctcttttt tattttagga attagagagg gatttgtcct tctatgattt
     9481 tttgctataa tagggaagat gtttatttta tgaaagaata gttgagggaa tttaaatgaa
     9541 taaaaaattg atgcaaggaa cattttggct aacctttgct aatcttctat gtaaagtttt
     9601 aggggtagtc tatttgattc cctggctaag tatgatgggg aacaatcaag atggtatgct
     9661 tgctacgaca ttgtataatg ttggctatct tccttatgga ttgttcctga tgcttggtac
     9721 agtcggattt ccaaatgcga ttgcaaaaaa agtagctgta gccacaaaag aaggagataa
     9781 cgctgcttgt cggctgattt ttagaagtac gatcaatatc atgtttgtca tcggtatcgc
     9841 ctctgctgca ctgatgtatt tatttgctcc tttactagcc ggaatcagtc cgattgctaa
     9901 cgttaataat gggatactag ctatcagaag tttatgccca tctttgattg ctatcccaat
     9961 tcttagtgca atgagaggat atttccaagg aaaaaatgat ctgcggcctt atggaacttc
    10021 tttgattatc gaacaggctg tgcgagtgat cgttatcttg gctggtactt attacttgcg
    10081 cgtattgact gacggaacca tattagaagc tgtattgatc agtacagtcg cttctttctt
    10141 cggtgggtta gctgcgatta tccatatgta tatcgtcgga cgcagaaaag acttcttcga
    10201 acttaaagat tttttgattt ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt
    10261 ctctatcatt cgggaaacac taccgtttat ttttgtcggc tcagtcatta ctatcgttca
    10321 aatgatcgac caagtaacga tgaaaccact gcttcacttt ttccgtccag agatcgcaga
    10381 tcagcaactg gaattcttat tcagccgtgc ttctgtcaat ccaaataaat tgacattgat
    10441 cctgatctcc atggtaggaa cagtagctat aagcagcttg ccaatattaa gcacgatgaa
    10501 gaaaaaagat cggatacaaa tcgaaaaaac agtaggagat agtttttcga ttgcgttatt
    10561 gattcttctt ccttcattga ctggaatgtc tttattagct ggaccactat acacattgtt
    10621 ttttggctat gatcctgaaa gtgtcggata tttccaaatg gctttgctag cttctttgtt
    10681 tttctcttta ttcacgatcc tatctacgat gcttcaatct ttgaatcacc atcgttttgc
    10741 gatccgatta actgtagaag caattatcct gaaagtcatc ttccaagtaa tcggattagg
    10801 attagttggc ggatacggga tgagcttatc aaatggctta gcattcggta ttgtctttgt
    10861 tcgcggctat cactttatgt gcaaagaata ccgaatagca cctttagcaa agatcagcaa
    10921 ttttttctta aaaactttcc gctccaccct gattatgctt gcagtatgtt ttgccgtttt
    10981 ctttttgctg aatcagcgat tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt
    11041 ggctgtagga agtattggcg gcttagtatt tttattttct caattcggaa aaaatgggtt
    11101 tcgaatgttg aaaaatgtta aacatcatag cagaagcaaa gaataggtta actaatcgtt
    11161 ttttgctgtc acttgaaaaa cggcaactcg tatgattacg cttgtcgttt ttctattttc
    11221 caattttagt ttcatatcta atcttactta tgatatagtt ataaggtctg gaaaaattct
    11281 ttgaaacgag gaaattaaac acatgaaact tgcagtagtt acagatagtt cggcatatct
    11341 gccggaacgt atcaagaacc atcccgactt attcgttatt ccaatccctt tgattatgga
    11401 tgggaaaatt tataacgaag gaatcgatat cgaagcggat gaatattatg gacttttaaa
    11461 aaacagtaaa gattttccta ctacttcaca acctgtcgta ggggaagttt tgggtttata
    11521 tgaggaattg aaagagaaag ggtatgacac gattttaagt atccatcttt cttcagggat
    11581 ttctggcttt gtcaacacat tatttgcgat gaaagatgac atcagcggag taacggtcat
    11641 tccttatgac tctaagatca ctagtatgcc gatgggacac atggtagaag ctgccttaga
    11701 cttaaatgat gaaggtcagt cccttgatga aatacttgca catatcgatc gaatccgaga
    11761 taatacctac gcttatctga ttgtagatga tttgaacaat ttagtacgag gaggacgttt
    11821 gacaaatggt gcagcgctga ttggcagctt attaaaaatc aaaccgatcc tgacattttc
    11881 tgaagggaaa attgttttat tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc
    11941 tgaagaaatt attggtagaa gaaataacga aatacaaccg ccagtcaaac tttacgtgat
    12001 acatgcaaat aatttagaag tagcgattgc ggagaaagag aaattgcaga aaaaatatcc
    12061 aaatgcagaa atcgaaattg gtcatttcgg accagtcatt ggtacacatt taggtgaaaa
    12121 agccatcggc attgccattt cagcgcaata attcctttta ttcaaaaaac ataccaaaaa
    12181 tattccaaca tcatcttgga ctttctacat gttactaatg agcgtgtaaa agaacgagat
    12241 ggtgttttta tttctatcaa tgaagctctt tttctttgca atcctcactt caatcctcta
    12301 taatgtcgca tatagtttag taaatgagta aactactaat ttacttattt actaaaaata
    12361 aaaggatgag gagttaaaat aatggaaata ccaacaaatt taaaacataa accagtaatc
    12421 gttgtagaag actatgataa agttgatgga agaaatgcat taaatacgga tgcaaaaggg
    12481 ctttcactgg gccttgctca gtggaatgat cgtggaaaag tcgatatctc aggcaaggta
    12541 tggcgacata ctggagaaaa atggtcacga cagtcggaag aattaccaat cacacgtatt
    12601 ttagatttag cgatcttagc tgcacaagga agtttatact tccaagatgc ttatcgttat
    12661 gaaaaatttt atgatccaga aaatccagtc gtagacatca ttggattaca agggaaccga
    12721 atgacggtag aagtagatac caaaaatcca aagatcgatg aagacatcat tttgtattac
    12781 gatacactac aaaaagacgg agaactgatt ggggaacgat tccgtatttt aaaacgtctt
    12841 ttagatgagc taggatatta atagacagag atgatcaaaa ggggaagaga agcatggaga
    12901 atttctcact aacagaaaac cgaacctttt cacggctaga aaagcaaatg atctggaaga
    12961 aaccgaaaag ccatcggaca agtgcagaag aatggcggat cgcctccgaa atcaaaggga
    13021 attggcaaga tcctgaaatg aaaatcagta acgtactttt ggaaggagat gccgggtcgg
    13081 ggaagacaca attagccaaa gcactatctg ctgatctgca acttccctat acaaaagtga
    13141 cctgttttgc agatatggac aaatcagatg ttttcggagc cttattacct gtagtagatt
    13201 ctaataagga acaagaccaa gaattactag aagctattta ccaaacagat acacttgaag
    13261 ctgtactgac actgatccag aaccattacg gcattgatcc tttttctgct aaagaaaaat
    13321 tagcagagct agttcaaagg atcgaagctg attatccaga tgcaatccat tacagctatt
    13381 acccttctga aatcgttcga gcgattgaaa aaggatattt attagagatt caagagccga
    13441 ctgtcatccg agatgcctct gttcttgtgg cacttaactc agcgctcgaa ccaaatggga
    13501 tgttgaattt accgacagga atcattcgtc gccatccaga ttgcgtcatt gtgatcacta
    13561 caaatcgaaa ttaccaagga aatcgcccat taaatgaatc tttaagagat cggatgcaac
    13621 atgcggaaaa aatggatctt cctgccttag aagtcatggc agaacgtgcg atggcgaaaa
    13681 ctatgatcca aaaacaagaa cttctcttaa agatggcaga gattatccgt ttgttggatg
    13741 aaactgcaaa agctaacgcc atcaaaggtg tagcaggaat gcgttcttat ttttattggg
    13801 caaataccta taagcaagga caagatttac tccagtcgat ttatccaaaa gttttgtata
    13861 aattatccac tgattcagat gaattggcaa ttcttgaaca agctttgtct gacagtggat
    13921 tgctactgga acttcaggac atattgagaa aagaaaaatg gggtcatcct caatctgagc
    13981 atacaaaagg aagaaccatt agcgccgaag aagcaaatga acgaaacatc actgaaaatg
    14041 ctgaagaaat tctgcgctta gatgaaaaga aatcgttgtc tgctgaagaa ccaaatgagg
    14101 gagaagagaa acaagaagaa acaatagaaa aaaacgagat tcctcctcaa atgcaaaaag
    14161 aacgcagtag cgatatcgaa ggaaaaaatc aaaaagaaca acaaacagga gatgattctc
    14221 aagaaatgtc tgcttctgac atggaagcac atgataagga aatcaagaag caactgaata
    14281 aagaagcgcg acaaatcatg aaggaaacga ttcatgaaaa agaaggtctc atcgttcacc
    14341 gaccagcttt ccaaggaaaa acgccggaag cacgtgagct acacatgcaa gtaatgccaa
    14401 tagtagaatc acttagccgg caaattttag agttactgga taatgaacaa acagagacgt
    14461 accaaaaagg aaaatacgaa ggtcagcggt tcaatgcaag caaagtagca tatggtgatt
    14521 tgagaaattt tgacaaaaag aacccgccac atgagcaacc ttctttagcg gtagctgtac
    14581 ggatcgatga atctgggtca atgatacggg atgatcggat cgaagcggca aaaaaagcag
    14641 cgattgccat tgccgaattt tctaagaaag tcgatatccc attgttgatt tacggagata
    14701 cagcagaccg ttcgtcaaga gaaaagactt ctttgttttc ttataaagaa tttgaagacg
    14761 gatttcaatg gttaaatgaa aaattagtca ctatgaaacc aaggcaaaac aatcgtgatg
    14821 gtgctgcact tcgtttagca gcagaaaaat taaacagaca atccgcaaca accaaactgt
    14881 tgcttaatat tagtgacggt cagccaaaag cgttgccgga ttatacaggc aaaaaagcaa
    14941 aagaagatat ccagtctgtt ttgaaagaat atgaacgtca aggaatccta tttgtctccg
    15001 cagctattgg ccaagacaaa gaagaaatca agtcgatcta cggtgaaagt cgttttgtag
    15061 atattactga cttgaatgaa tttcccaagc aaatgattca actgattgcc cgctatctat
    15121 gataaaataa agctcaacga acgcacttag cagattcgtt gagcttttcg tttctagtta
    15181 ccggtaacaa ggatacttct aaaatagaat cggttataat caagagggac cgttaaagga
    15241 gtgaaacgat atgatacaag aacaaaaatg gtggaaagaa gcagtaggct atcaaatcta
    15301 tcctgctagt ttcaaagata gcaatggcga tggtcaagga gacatcaatg ggatccgtga
    15361 aaaacttaat tatttgaaag atcttggtat cggctttatc tggatcaatc cgatctatca
    15421 atctccattt gtcgataatg gttatgacgt aagtgactat caagatatag aagagagatt
    15481 cggaacgatg gaagattttg atttattgct aaaagaagca catgaattag ggatcaaagt
    15541 gattatggat cttgtcatca accatagttc ggatcagcat caatggtttg aagaatcgaa
    15601 aaaatctaaa gaaagcccgt atcgcgatta ttacatttgg gtcgacgggg tagatggcaa
    15661 agaaccgaat aattggacat cgattttcgg cggttctgct tgggagtatt ctcatgaaac
    15721 tggacagtat tatctgcatg tttttgcaaa agaacaacca gacttaaatt gggaaagcga
    15781 gaaattgaaa gaagaactat tcaatatgat ccgatggtgg ttggataaag ggatagatgg
    15841 atttcgttta gatgcgattt ctcatgtaaa aaaagatgaa tattcagtaa aagccacaga
    15901 gaaccctttc tcaccctttc aaaatgtctc aggaatcgaa gaacatttga ccgatttgaa
    15961 acatgttttt gaagagtacg atatcatgac tgtcggagaa gcaagtggtg tcactgcaga
    16021 agaaggacca caatgggtag ggaaagacgg ttatttcgat atgatattcg aatttgatca
    16081 tatccatatc tggcagcaag aaaaagaagg gcaattagat gtccttaaat tgaaacttgc
    16141 attaagcgct tggcaaactt cattggatgg tatcggatgg aacgctttat atatggaaaa
    16201 ccatgatgtt cctcgtgccg tttcagtgtt tggagatact cgtcccgatt tttgggctat
    16261 gtctgcaaaa gcaatcgcta tgatgtattt ctttttgcaa ggtacgccat ttatttatca
    16321 aggacaggaa atcggaatga cgaatatgcc ctttgaatcg atcgatcaag ttgatgcagt
    16381 agattcgaaa agactttata aaaggctgct tgcggaaggt aagacaagag aagaagcatt
    16441 ggatatcatt cgtgaaacaa cgagagacaa cagcaggaca cccatgcaat ggacttcgga
    16501 acaatatgct ggtttttcta cacatgaacc ttggctgatc actaatccaa ataccaaaac
    16561 gatcaatgta gaacaacaag aatatgaacc agagtcggtc ctgcaattct ataagaacat
    16621 gatccgcatt agacaaacca ataaaggtct gatatacggt tcttataaag aatatcttca
    16681 tgaacatcca caactttatg tctatgaaag atatttagaa gatgaacaat atcttattat
    16741 ggtaaactta acagaatcac ttgctgacta tgaacttcca aaagaagcag atcagtcctg
    16801 gacattactc ttgtcaaatt cctctagcgg agaatttgaa gctaaaggca tactagcacc
    16861 ttatgaagct cggttatata aaacaaacaa gtaaaacttc ctcgttaaag ttcattagtt
    16921 ggactttgcg aggaagtttt tcagattgct ctgtcaatta ctcatttcaa taattcgctc
    16981 taggaccacc gatatattta ggacgagagc gaagggctgt cacgtgagct tgtccaaggg
    17041 ttttcaaacg aggtctgaca ggtacttgga catgtttgat atgcataccg attgatgtgt
    17101 caccgatatc gataccggct tgtccaacta cgtgttccac ttcgacagga tcattgaact
    17161 ggtcaaaagc agcaacagaa caagcacctc cagcatgtag tgcaggaaca acagagacaa
    17221 tttcgaaatt ttttgcttct gccactgttc gttctactac taatgctcgg ttcaaatgtt
    17281 cacaaccttg gacagctagc gcaatttctt taggatccaa caactctttt aacgtacgga
    17341 tgatccattg accaacttcc gcactggaat tttttccaat atgtcctcca acgacttcgc
    17401 ttgtgctgca tcctaaaaca aaaagatcac cttttttcaa gtgtgcctca gccaatatgt
    17461 cattaactat ttcagtcaat tggtctttta attctttttc tgacaacttc attttctaaa
    17521 accttctttt tcaattaata ttgcttccgt gcttgaatac gttgaagact tgcagttata
    17581 ggaaaggcaa gtcccattcc aatcagattt tggaagatgt ttcctggtat agaagccact
    17641 ccagccggcc aatcatataa aatccaaccg gcgataccat agccgatgat cataactaag
    17701 cttcctaata aaagccctaa tccccacata cgagtagaag atttgctgaa agaaccagca
    17761 acatatcctt gtaacccgtg gatcactaat gaaaaaactg cccactgcgg atatcctgaa
    17821 atcaaatcga tcaatagacc actggaagca ccaacaacta atccacccgt tggaccataa
    17881 agaaaagcag cggtatagat cccggcctcg cataatgtca caaatccatt cgtagccggt
    17941 acagggatca aaattgttaa tgaaagggca actgtcaacg caatcagcat agcatagatc
    18001 gttaattgct tagttttcat ttctcattcc tcctttttga ataccgccag accagacact
    18061 tccggtatga tcgttcatca gcacgccatt agaaatacaa tgggcaacaa attttttact
    18121 ttctgctact gctttggtaa aatctgcccc taacacaaga ttcgctgtta ttgcagaaga
    18181 aaaggcacaa ccggccccgt ttactgttgt agtgtcgatt tttttatgag aaaatacctc
    18241 ataatgatca attgaataga gaagatccac agcttcatct ccagccatcc ttgtgccgcc
    18301 tttgatcaca atgttttttg ttcctgaagc agtgagttct ttagcgagat ggatcatttc
    18361 tgttaacgaa gagggaagag cttgaccaga aagtaaagca gcttctgcca aattaggcgt
    18421 aatcatcgta acaagtggaa aaagttcttc gatgagctgc gaaatataca attgattgat
    18481 cacagcttca gtttctttga atgccaaaac agggtctaaa atgattggga tattttgttt
    18541 tgtttttatg aagtctcgaa caaccatgat tgcttcactt gaattcaata acccaatttt
    18601 gataccatct aatgaagtca ttttatcaat cgaaactaac tgtcgtttaa gcaattccaa
    18661 agatatagat tccagcataa atcgctcatt aatatctagt gcccctatag aagtcaaggc
    18721 gcttagtccg aatagaccaa aattctcaaa agtttttaaa tctgtttgga ttcctccacc
    18781 agcaaatgga tcagaaccgc caattgtcaa gattcttttc ataacatttt cctccatgaa
    18841 taagagtata acgaaatttt caaaaaagaa aaacagccaa ttggcatatt tttatctcat
    18901 ccaatttaaa aaaacaaaaa gaaaagaaag accaaaatgg atctttcttt ccttaaatag
    18961 ttaggacttt tgtgatttta attctttctc gatttgaagc cgttgcactt tcattgtagc
    19021 tgttcgcggg attgcatcaa aatcacggat gatagggacg ttcaagcgag gaagttcatg
    19081 gacttgctcc caccatcgat tccaatccat ttctttatca ggagctaaag caataatcgg
    19141 ttgtggagaa tgatttttat cccttacaat cactacttct gccaaaaaat ctaacgaatc
    19201 taataaaaag tcttcaatag ctaatgtact attgatcgtt tcgatcaaat ccacttgccg
    19261 atctttcaaa aataggtggc catgcttatc cataaaaccg taatctccgc tatcccacca
    19321 atcgccataa acattttcct ggaaccgtgc atcttccttg taataagtca aagcccgtcc
    19381 tttagacagt aattgtatat gaccatctgt gttttcaggt aggacattcc caactgaatc
    19441 tgtaatccga gcgcttgtca tgtcttccag tccaacgccc atatctcttg catcacttgt
    19501 tttcaaactc tctagtgtat gagccttaag aatcattggt ccgcattcac tttggccata
    19561 tacttgaaga aataccgcat cctgtgccat cgacgttcgc aaaaatgtct tcattgtctg
    19621 gttgtttata gcatcaaaag tggagtgata gtatcggata cccgaaaaag cttctggatg
    19681 ctccttagca gtaaaacgcc actgaacaaa gttattcgga tgggtctcta gtgcaattgg
    19741 ccggtgagct tccaacatat gaacaacttt tgaactttgt gcgtttgcta aaggcatcat
    19801 aggaaatccc atggacatca aagaagagat accaatattg aatctggaat gcactggaga
    19861 gatgtgaaaa gcgattaatt tcttttccga aatttttgtg aaaactgttt tttgccattt
    19921 cgtccgccat cccatagaat gagcagaatg acaaataagt ttagggatac cagttgtacc
    19981 agaagtatgg gtcatataag aaatttcatt tttgtctaaa aatgattgag aaacagacaa
    20041 agcaggttgt tccattaaat aaagaatag
//
LOCUS       DACDSY010000041        20022 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDSY010000041 DACDSY010000000
VERSION     DACDSY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.041"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            90..386
                     /locus_tag="IWK44_001218"
     CDS             90..386
                     /locus_tag="IWK44_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP9549755.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(1030..1983)
                     /locus_tag="IWK44_001219"
     CDS             complement(1030..1983)
                     /locus_tag="IWK44_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9549756.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(2006..2761)
                     /locus_tag="IWK44_001220"
     CDS             complement(2006..2761)
                     /locus_tag="IWK44_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9549757.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2758..3717)
                     /locus_tag="IWK44_001221"
     CDS             complement(2758..3717)
                     /locus_tag="IWK44_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9549758.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3710..4660)
                     /locus_tag="IWK44_001222"
     CDS             complement(3710..4660)
                     /locus_tag="IWK44_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9549759.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4810..5262
                     /locus_tag="IWK44_001223"
     CDS             4810..5262
                     /locus_tag="IWK44_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP9549760.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5351..7618)
                     /gene="gshAB"
                     /locus_tag="IWK44_001224"
     CDS             complement(5351..7618)
                     /gene="gshAB"
                     /locus_tag="IWK44_001224"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP9549761.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7805..8803)
                     /gene="ftsY"
                     /locus_tag="IWK44_001225"
     CDS             complement(7805..8803)
                     /gene="ftsY"
                     /locus_tag="IWK44_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP9549762.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8814..9623)
                     /locus_tag="IWK44_001226"
     CDS             complement(8814..9623)
                     /locus_tag="IWK44_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9549763.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9632..13213)
                     /gene="smc"
                     /locus_tag="IWK44_001227"
     CDS             complement(9632..13213)
                     /gene="smc"
                     /locus_tag="IWK44_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP9549764.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13233..13919)
                     /locus_tag="IWK44_001228"
     CDS             complement(13233..13919)
                     /locus_tag="IWK44_001228"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9549765.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14267..16048)
                     /locus_tag="IWK44_001229"
     CDS             complement(14267..16048)
                     /locus_tag="IWK44_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9549766.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(16074..16988)
                     /locus_tag="IWK44_001230"
     CDS             complement(16074..16988)
                     /locus_tag="IWK44_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9549767.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(17015..17977)
                     /locus_tag="IWK44_001231"
     CDS             complement(17015..17977)
                     /locus_tag="IWK44_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9549768.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17977..18921)
                     /locus_tag="IWK44_001232"
     CDS             complement(17977..18921)
                     /locus_tag="IWK44_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9549769.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18922..19926)
                     /locus_tag="IWK44_001233"
     CDS             complement(18922..19926)
                     /locus_tag="IWK44_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9549770.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5575 a         4288 c         3556 g         6603 t
ORIGIN      
        1 gaaaaagaat tactttttgt tcacggatta ttaacaactc ttcggtagag taaacttatc
       61 aaataaaaga cttctttgga ggaatcacta tgaaggaata tataaaaaac atctatttta
      121 tcgaagaaac acaaaatatc gagggttctt atattgaagt aaaaacactc tttgtcaatg
      181 aagataaaac caaagcattg gatatttata aaaaattagc ttcaaaaaaa acgaatagtt
      241 ttggattgat attaagcgag tataaaatca aagcagaaga gagttatttc tatcaattat
      301 taaaacggtg gagtaagctt cccgccgatt tttatcgaaa aatgcagatc atcaactatc
      361 aaccgcttgc tgagacacat gcctaaatag aggttgtgaa atcagtgctt tgagctaata
      421 ccgaaggtca gctgattgaa cattgtttag aagagattgt gacaactgcg atttgctttg
      481 agtaataaga acgctggaca gaaaatagct tctcatattt tctagtctag tgggcttatt
      541 gccgagaagc agcagatgga acatcgttta gaagagattg tgacaactgc gatttgcttt
      601 gagtaataag aacgctggac agaaaatagc ttctcatatt ttctagtcta gtggacttat
      661 tgccgagaag cagcagatgg aacatcgttt aaagaagaga tcggtacaaa ataaaaatat
      721 ctaaatttaa gttttatcta agtttcgctt tgtacctttg attcaaatca aaggaggcga
      781 tcttgatgct gaaaaaatgg atcacactat tttttgccac tccccattct agaatcaacc
      841 cgtatgatgt agataatgaa ttcacgttga aaaaagattt ctcaaataaa taaaaaccat
      901 ccgtaaaatc atcaatacga ttttacggat ggtttttgat aacttctttg aggataggct
      961 cttgctattt aagaatgtaa gaaacattat tgttacatag tatgagagtt taatcctctg
     1021 ttatttatgc tatttaaatg cttgattcac atcttcgatc atcagtttca ttgattctaa
     1081 accgcccccg cttaaatacc agacatctgg ttcaagggag atgatctgct gatttttacc
     1141 agcattcgtt tgtgctacca gttcattggc agaaatatca tctttgctgt cgtctccacc
     1201 aatagctttt gttctgtcta ctacaaataa gatatccgga tttttttcca gcacatattc
     1261 ataagaaaca ctttgaccgt gagtggatgc ttcgatttga tcatctgctt gttcaaagcc
     1321 aaataaatca tggatgaaac cgaatctaga accagatccg taggctgata attgcccttc
     1381 attcacaagt gtgactagtg cttttttgtc agtagcttct gctttctttt tcgtctgatc
     1441 gattgcacta gataattcct ctaattgagt tttagcttct tcttgcttac caaagatctc
     1501 ccctaaagca gtgacatttt gctggaaaga ttcccatgga tttttcgcat ctaaagaaag
     1561 gaaaatggtc ggagcaattt gacttagatc tttttgaaaa tctctttgtc ggccagaaat
     1621 gatgattaga tctggctgaa gctgattaat tttttctaag tctggttctt ttattccacc
     1681 tgctgattct acttttttat aggatgaaag atactcagga aggctgcttg tagctgctcc
     1741 aactacctta tctccaacac caagagcatc catcgtatct aaagaaccat tgtcgaatac
     1801 gacgactttt tctggattat ttggcacctc gatttggtct ccattactat ctgttatcgt
     1861 caatgtagtt tcagtttgtg tgctgctttt ttttgtgtct gcctgactgg tgttgttgct
     1921 atttccacat gctgctaaac caaatgatgc tagaatcaag atacctacta ctaatttttt
     1981 catctcttct ctcctaatgt tagttttaga aataaagaca gaatctgcgg ccttcaatct
     2041 cacaaatccg tatgtccatt tcgtataatt catccaatac atcttttttt atcatttctt
     2101 gggtcgttcc ttgcgtaaat aactgtccat ctttcattgc aacaatttcg tctgcataac
     2161 ttgcagcaaa gttgatatca tgaagaacaa taacaattgt tttcccgatt tcttgtacta
     2221 aattttttaa aatctgcatg atttgaaccg caaaattcat atctagattg ttcaaaggtt
     2281 catccagtaa aatataatcc gtgtcttgcg ctagcaccat tgcaatatac gcacgttgga
     2341 gttgaccgcc tgaaagagtg tcaatcgatt gatccgctat ttcttctaac ccaaggtgtt
     2401 ccagtgattc ctgaacgatt tcctcgtctt ttgtcgtcaa ttttcctttc gtataaggaa
     2461 atcgtccaaa ttctactaat tctttcacgg tgatttggac atttatttca ttcgattgtc
     2521 gtaggatcga taattttttg gctaattcat tggatttcca agcctttact tcgttatggt
     2581 ctaaatagat ttcacctgta tcttttggta tcaaacgact gatcatcgac aacaccgttg
     2641 actttccagc accatttgga ccgataaaag cagtcaattt cttttcagga atcgttaact
     2701 gtacatcttt aaccactgat tttccatggt acagtttatt gaccttttgt aaatcaatca
     2761 taggatactc ttcctttcgc gaatcaattt tgtgacaaaa taaattcctc cgacaaattc
     2821 aacgacgaca ctcactgttg tattccagcc gaaaatctgc tctactgcga gctgtcctcc
     2881 tgtcagaaac agcatagcta ccagaaagct tccgataaat agctgaccat gacgataagt
     2941 agaaaagaca cgataactga tatttgcgac aataaaccct aaaaaagtaa ctggacccac
     3001 aagagccgtt gatgtccctg tcaaaagact gacacaacat aaaatcaaaa attcaaaacg
     3061 atctgtgtct atcccgaggt ttactgcttg ttcatttccg agatggaaaa cgtctagcac
     3121 atgtcgatat cgaaataaca ggtaccagca gccgataatg atcaaagaag caggcaaaag
     3181 atactgactc tggatattgc caaagcttgc aaataatctg ccttgaagca aatcatattc
     3241 attcggatcc atgatgactt gtaaaaaggt actgacacta ccaaaaagcg tacctaaaat
     3301 catccctgac atcagtaata aaaaaagatt cgctctcatt tttttcaaaa gaaagaaaaa
     3361 taacaataga ctcagcccga ccatcagccc aacgttcaat aagaaaaaaa ctgtacctgt
     3421 ctgctgaagc atcgtcactc ctccgataaa gaagaaaagg accgtttgga caaggacata
     3481 taacgactca agtcccaata ctgaaggagt caaaaactgg ttatgcgtaa ttgtctgaaa
     3541 gctgattgtg gcactgctga cagctagcga gacaaggata aatgcaaaaa ttttcttact
     3601 cctcaatgca catgcaaaat gccagtttcc atatgtatgg atcgtcaagt acattcctgt
     3661 aacaaataaa acaagaacaa gcataccaat aaaaatagat ggcttttttt taagcatatc
     3721 gtcgtcccct tatcagaaga taaaggaaga tagccccacc gatgattcca acgatcacac
     3781 tgactggtat ttcataagga aaaatcaaca cgcgagcgac aagatcgcaa aatactaaga
     3841 acaaactacc tgctaaagca gttacgccaa ttgttttgtg cacttgatct ccatatattc
     3901 tagagacaag attcggaatg atcactccga taaaaggaat gcttcctacc atgatcaaca
     3961 tgactgcact agccaatgat actaagccaa tgcctaaaaa ctgcatcttc tggtaatcta
     4021 ttcccagatt tactgcaaac gattcgccca gaccaatgat cgtaaatcgg taagcaaata
     4081 gccaaaccaa gagcaacaat ggaacaacag catacaataa ctcataattt cctcgtatca
     4141 cagtcgcaaa attcccttga agccatgaag tcatattttg taccaactgg aattgataag
     4201 caaaaaaagt agcaactgaa ccaatgatat tccctaacat gactccaatc aacggaagca
     4261 tcaattgatt tttctggagg aacaatcgcg taagggataa aaaaagcaat gttcctgcaa
     4321 aagcaaagca aaatgctgtc aaggatcgga caatcaagga tgcaccaggt aggaaaatca
     4381 tagccactaa aatacctaaa cgcgcactat ccatcgtgcc cgcagtcgtt ggtgaaacaa
     4441 atttattttg ggtcaaatgt tgcatgatca acccacaaat actactggtc gctcccacaa
     4501 ggatcaaaca aatcgttcgt ggcaaacgag tcactagcaa gatttgtaag ctttgcccat
     4561 ctaactggag cagttctgac aattgtacat tcgatactcc gatgaacaat gaaatactac
     4621 ttaaaataac taataatgct actagtcgtc cgtagcgcat ccaatccacc tttctaattg
     4681 agaatgattt tcattcttat tttaagtgaa tcgttgaaca aaagcaaccg ctttttcata
     4741 tgttttaggt attattggga atatgctata atcgtaagaa agacaaagag aagaaggagg
     4801 gtatttttta tggcacaaga tgatgtaaaa agccatttgg ataagggact ttatggtaca
     4861 cctttagtga accctgaaga acagcataaa tatttaggga cattcaggga acgctgctat
     4921 cttagtatga ctgttgctga aatgatccaa ccaaaaaaca aagaaaactt tttaaaagaa
     4981 ctcaaaaaac atccagatgc ttccatactc ttgaatgggg ctctacccct ttctgtccaa
     5041 aaccaatata tccagctagc aacgaaaaac aatagccgtt tcactgtagt caacgattac
     5101 gtcactgcaa atcctgagga attcggattg ctcttaacag caaaagaagc agtaaatgaa
     5161 cctgtgattg atatcgaaaa aaaataccca gaagaaaact cttcgccagc tgaaaaagaa
     5221 aaaccaaaaa agaaaaaaag cttctgggat cgtttatttt aaaacgaaaa aacacataaa
     5281 tttttacatg caaaaaaagc ttttgatcag attgacatca aataggacaa tctgtcaaaa
     5341 gctttttctt ttaaaatact tcaggatata acaatttcaa tacattcatc gtcaatcttc
     5401 tgccttttcc tgcaaaagga taaacatgca tatgcatggc tggattgaaa tttgcttcga
     5461 tgatcccata tgctttttta tcagttgtag gatcgatttc tttatctggt ataatcaaat
     5521 cgataccact aatcttagct cctagtgctt ctaccgcaga aacggcgatt tttttgtaag
     5581 cttcagaaaa ttcatcagtc atatcgatcg aatcaccacc tgtactgata tttgagttct
     5641 cccttagata aacaatttga ttggcttggg gaactgattc gatcgtcaac ccttgttctt
     5701 tcaacatcaa ttgttctaat tcacctaatt ggatcaattc taaaggtgca cgatgattcg
     5761 ttccacgcaa agggtcacta tttttttctt caacaagttc tttcactgtt cggatactgt
     5821 caccaataac attagcaggg actcgcaaca tgatggcttg gacttctccg tcgatcacaa
     5881 agaaacgata ttccgttccc ggcataaatt cttctactaa aaccgaactg tcttcacgaa
     5941 aagcaatggc taaacctgct tgataatctt ccagactagc tccttccttg aaaatcgtga
     6001 tacctaaccc gtaattggtt gatttaggtt tgatcacaaa tgcctgctcg gcaaatctcg
     6061 gataagcttt taccgcttct tccatggatg aaaattctgc gcctcctggt acccggaacc
     6121 cagcttcttt taagactttt ttcgttactg ttttgttttc catgataagt gggacgatat
     6181 agctgtcctt acttgtcatg tttgcatttt tcacatattc tacatggtct tggaattgca
     6241 gacgcaaaaa ttgatccgac tcatctaata ctttgacttg gacacctttt tggatggcat
     6301 cgaacataaa tatctgagta gaaagttcca tttcgcgaaa accggccaac tgatagggac
     6361 gttcatggct ttctttataa tattgatgtc ccaatgccac agcaacttct tgctgactcg
     6421 atagctgtgc ttcttgatag agtttcgctg ctaacgtttg ttcaggatga tccatttgag
     6481 tcaatgcttc ctctactaaa taaagagaat ccaaccaatc caattcaacc agcatttgct
     6541 tcattccttc aagaatttct ctaccttctt gatgaaattc tgtttgatct gatggttgtt
     6601 ctagtgctac tttgttattt cgagtcgtac cttctgctac ccattggtca cagtcttctt
     6661 tttcttctgt ccataacatg tacatcagga ataattgtaa gaaccgaact tgctctggat
     6721 tgatacccaa tcttgcataa ggattcaaat cgatattccg caattcaata taacgaacac
     6781 ctgttgttgc taaatctgcg actttctttc cgcctcggaa acgaagcggt gtataaaatt
     6841 ctttctcctc cgaaagtttc ccttcttcga tcattcgctc gatatctgct aaatactgtt
     6901 tcatagaagc ataggaaact ttcacatttg gatgattcgt atagcccagc gcactgttgc
     6961 gaatactgcg aacaggttct tgcggatgtg attcatctaa aaagtagttc ttctcactca
     7021 ttggtgaagc accgaataaa taagtgatca tccagcgata gcgcataaag tttctcgctg
     7081 ttttcaaata aagttctgtt ttgaattcgg aaaaatcttg aaattcttcc tgatggctga
     7141 acaatgttct tagcaactca tcgccaaatt caaaattgaa atgaataccg cttaccatct
     7201 gttttcgctt cccgtattct tttgctaaat aacgtcgata caagacatct tcgaaatttt
     7261 ttaattttgc aataatgatc tcttcgtctt tttccggtaa ggcaggtggc atacttaatg
     7321 gccagatcat ctcttctttc ggtatagaac gagcagtcac atcataaaca gcagcgagat
     7381 actgaaataa ttcgggaata gaatcggtaa caggggtgat catctctgtt tgtgtttcgc
     7441 taaaatctgt ttggatatag gggtgatagg atcgatcgcc aaagattgct ggatgatcgg
     7501 tttttgctag atttcctgca agatcaaccc gctgtccttc ccgttctata ccaaatcgag
     7561 cttggtcgat aaaagggcgc gcattgacat gcaataataa ttgcttaaaa ttcatcatta
     7621 agtgacctcc aattcatatg aatctaatta taatcaaaaa gtctaagaaa aagaacgaaa
     7681 ttgtttgaga accgcttaca aataagtata aaaatgaagg catcaaaaac acagctatgt
     7741 taaaaacaat cattcaaaca aggagctgca acttttgtca cagctcctgc tttttagaca
     7801 tgggttattc ttcttttagt aatcctttga acaatccaac gacaaaatca tttggatcaa
     7861 acggctccaa gtcatcgatt ccttcaccaa gaccgaccaa tttcactggt aaatgcaatt
     7921 cattacggat agcaagtacg ataccgccct ttgctgtacc atctaatttt gtcaaaacaa
     7981 gaccggtcac atctgttgtt tctttgaatt gttttgcttg aaccatcgcg ttttgaccag
     8041 tggttgcatc taatacaaga agtgtttctt ggggtgcttc tggtaattcc cgctggatta
     8101 ctcgttttat tttttctaat tcgttcatca agttcacttt attttgcagt cggcctgcag
     8161 tatcaactag taaaacatct gcctgctctg tctttgctct ttccaaagca tcgaatacga
     8221 cagctgctgg atcgcctcct gcattgccac gaacaacttc aacaccagcg cgttcgcccc
     8281 agacaactaa ctggtcgatt gctccagcgc ggaaggtatc tgctgctgcc atcaatactt
     8341 ttttaccttc gtttttgaac tgatgggcaa gtttaccaat acttgttgtt ttccctacac
     8401 cattaacacc gacaaacaaa ataactgtta gtccgtcttc ttggatattt agttcattta
     8461 cttcattgat tccttccgct tcatagagat ccacaagttt ctcgatgatc gcattttgga
     8521 cttctgccga ttttttcgca tttctgagtt tcacttcttg acgtaatgct tcggtaattc
     8581 gtatcgctgt ttcaaaacct acatctgcac cgataagtgt ttcttctaat tcttcaaaaa
     8641 agtcttcatc gactgttcga aaattggcaa acaactcatt catccgttca ccaaatgttt
     8701 tacgcgtctt ttctaaaccc ttgtcgtact tctcctgtac ttcaacttct tctgttttct
     8761 cttcgccagt aaaagcacgc ttgattttat caaaaaatcc catttgactc cccctactct
     8821 aatacaaatt tttggatcac tttggctacg ccattttctt cattgctggc tgtcacgaaa
     8881 tctgctgctt ctttgacgaa aggcacagca ttctccatcg ctacgcctag tccagcatat
     8941 tggatcatcg gtaaatcatt ttcctcatca ccgatcgcca tgatttcttc ttgtttcaaa
     9001 ccaagatctt ttgccaataa agaaatacca taagcttttg taacaccttt tggcataaat
     9061 tccaaaagca tacttcttgt tttgatgatc tcatacattt catggaattc tgctggtatt
     9121 tctttgattt taggatctaa ttcttcttgg tcataagcga ttactgcctt attaagcaca
     9181 aattcatctg taatatcgct tatatccgct ggttggaatt gcagcagctt attcaacaca
     9241 ttgtacaagg attgcttcct tggggcacta ggcagctgta aaaccacatt gtcagacaaa
     9301 acatctagtg gcagatccaa ttgctgagcc agtttgtata agcgttggat atctgatact
     9361 tccatcactt tcttttccat gatttcacca gtatcatttt tttgtaccaa accgccgttg
     9421 aaagtgatgc tgtagtcacc ttcatctacc agtcctaatt cttgcagata atgcgccatc
     9481 gcagctaagg ggcgtcctgt acatagaacg atcttgaccc cttgctgttt tgcttgtgct
     9541 aatgcttgtt tattctcatc tgaaatcagc ttttgttcat ttaataatgt accgtccaaa
     9601 tcgattgcta ctaatttgat cattttcgtt cctacacttt cgtttcgttg attttcgcct
     9661 cttcattgat atcttccatc cgtacagaaa cgatttttga tacaccggat tcctgcatcg
     9721 tcactccgta cagtacatct gctgcgacca tcgttccttt gcggtgtgtg actacgataa
     9781 attgtgtgtc atcttggaaa tcgctcaagt aacgaccaaa acgtttcacg ttggcttcat
     9841 ctaacgctgc ttctacctca tctaagatac aaaacggaac aggacagaca cggataatgg
     9901 aaaagagcaa cgcgatggct gtaagtgctc gttctcctcc agaaagcaaa cttagacttt
     9961 gcagtttttt gccaggaggt tgtacttcaa tctcaatacc tgttttcagc aaatcagttg
    10021 gttccgtcag tactaattca gctttaccgc cgccaaacat atttggaaag acaattttga
    10081 attcttgacg gatagcttca aatacttctt tgaatcttgt ccgcacttca tcatccatct
    10141 catccatcgt ttcaaacaat tggtttttcg ctgctaacag atcatctcgc tgtgttgcta
    10201 agaatgtatg tctttcactg acttgttcgt attgttcgat tgaatttaag tttactggtc
    10261 ccagtttttc aatctgttct tttaaggatg aaacctttgt tctactgtct tcgatatcgg
    10321 ttgtttcttg atagtcttgg gaagctttct caaatgtcag ctgatattct gactgcagat
    10381 aggtcaaatg attatctaaa atcatttcag ctcggtcttt ttggacttct aatttggatt
    10441 gttccgacag tcgtgctttt tgctcacgat tacgctctgc caagactgct tcaagcttat
    10501 cgatctcctt ttgccttcta tctcgctgct ctttggcttt tgccaactcg gcttttaatg
    10561 tttctcgctg agcagataat tcgttaattt gtttttccaa actttcttct gtgacttcat
    10621 gatcagaaaa gtctgctgtc aatgtcgcca actgcttttc tatcgcttct tgacgttcgg
    10681 ttgcctctgc tttttggact cgggcaccgc ggagctggat ctgcagatga ttaaactgct
    10741 ctttcaagac agcaagatct gcttgttctt gtgctttttg actttgtact tgtgctcttc
    10801 tggcttccat ctggtcgctt tcttgactta acgatttgat ttcttcgtcg attttttgtc
    10861 gttgcgattc aagatctgtt tgtttgtctt tcaattcgtt cgtttgcttt tcgtaatctt
    10921 cgataaactg ctgcagttca cgtgtctcaa aagttgagat ttgcttttct ttttcaaaac
    10981 gttcaagttc atttatgatg ttttgtagct gatttgtcgc ttcttgttct tcaaaacgta
    11041 attgttcgcc tcgtgtacgc aaaacttctt gttcttcggc taaacgagct gtttcttgtt
    11101 gcagctcctg aacttttttt tcttgtgctt ggagttgttt atctgcttct tcaaattcac
    11161 tagtcaactg tttcaattct tgattttgta caaacaaact gccttggttt cctcgtttat
    11221 tcgctccacc tgtcatggat cctccggcat tcatcacatc accttctaag gaaaccactc
    11281 gatattgata gcggatcgtt tgcgcgattg cattggcact agttaagtct ttggctaaaa
    11341 ggatcgttcc tagcaggttt tgaaccacag tttggatttg atcgggataa ctgacttgtt
    11401 cactggcaat ccctataaac ccttctacag ctgctgcttg tgtcaatata tgagccggta
    11461 attgtcttgg tttgattgtc gtcagtggaa ggaatgttgc tcttcctcct ctttgctgtt
    11521 tcaaatacgt gatggcttgc cgagcatctt tttcattttc aacgatgaca tgctgggctg
    11581 ccccacctaa agctgtttcg atagctaacg tgaaatctgc aggaacatcg atcagttcag
    11641 ccactgctcc gacaataccc gaaagctgct gtttatgctg caagaccaat cgaacccctt
    11701 ggtaaaaacc aaaatagttt tcttggattt cttgaaggct tttcttacga gcacgtagct
    11761 gctggacttg attcattaat tgatacattt taggctgttc ttttgctaat gcttcttgga
    11821 actttttccc gttttgttgg atcatttcta acttctcttt tgtttcagtt aaagatgatt
    11881 ttagctgcgc ctgcttttgt gtcaattctt ctttttgtaa gatcaatcgg tctactgatg
    11941 cttctacttc cgattgcttg gctaacgtct gcttgctttt tgctgtttct tgtatatatt
    12001 gacgttctag atacttcagt tcgtttccta cagctgcttg ttcttgcatc aaatcaacat
    12061 attgatctct taattctgcc aataattctt tcgttgattt ttggtatttt tctaattctt
    12121 gctgtgtttt tatgagattt gcttcagctt tttggatctc tgtttctttt tctgctgctg
    12181 ctttcatcaa gctttcttga agcttttcaa aatgtttaac ctttttttgt gcttctgcta
    12241 atgaagtttg atattcttgc gagctttttt gtgtatgctt tgtccgctcc tgcaaaacat
    12301 ctttttgtcc ttctgtctgc ttcagttttt ccgaaagatc taataatact tgctgatttt
    12361 tttcgatcag acgatcggct tgcgcatttt ctttacgctg cttcgccaaa atggattctt
    12421 gttcttggat cgactcagat agtttgccta attctagatt gaatttcgca agctgtgctt
    12481 gtttgttgtc ccaatctttt tttgctgtct tgatttcagc aaccatcaaa gagacatcgg
    12541 tttgagtcag tgtttctttt agccgtaaaa actcctttgc tgcctcactc tgggcggcca
    12601 aaggagttaa ctgttcttcc aattcgtgga tgatgtcttg gacgcggctg aggttgtctt
    12661 ctgtttcaaa aagcttctgt tctgctttct ttttccgctg cttgtatttc aagacaccag
    12721 ctgcttcttc aaagatccca cgacggtctt ccggtttact actgaaaatc gcttctactt
    12781 ttccttgaga aatgattgag aacgattctt ttcctaatcc agaatctaag aacaattctt
    12841 ggatatcttt taatctgcag ctttgtttat tgatgaaaaa ctcgctttcc ccagtccgtc
    12901 gataacggcg ggttacactg atctcattgt actccaatgg cagataatga tcactattgt
    12961 ctaaaatcac tgtgacttct gcaatattca gctgcttacg ggtatcggaa ccggcaaaga
    13021 tgatatccgg catttttccg cctcgcagac ttttagcaga ttgttctcct aatacccagc
    13081 ggatagcctc tgtaatattg ctttttccgc tgccatttgg tccaaccact gctgtgacgc
    13141 tattttcaaa atcaatgact gtcttgtctg caaatgattt aaaaccggct atttcaattc
    13201 gtttcaaata caaagctttc cctcacttac ttttacagtg ctgccaatgc attttccgcc
    13261 gcagcttgtt ctgctaattt tttcgatttc ccttgtccag tgccaatcaa ttgatcatct
    13321 acataaactt cgatccaaaa tactcgttca tgagctggac cttcttcatt gatcaatcgg
    13381 tattcgattg atacatcacc tgatttttgc aagacttctt gtagttttgt tttatgatcc
    13441 atctcatgtg aaaaagcacc tgctttgact ttaggaaaaa tcactttttc gatgaatgca
    13501 tgggtcgtgt cgaatccctg gtcaagatat aacgcgccta aaaaagcttc gaataggtcg
    13561 caaagcaatg ctggtcgcgt acgtcctcct gaattctctt ctcccttgcc caacaaaaca
    13621 tattgatcaa agtgacattc tttggcaaat ttcgacaagc tgtcttcgcg aacgatcgca
    13681 gctcgtgttt ttgtcaattt tccttctggc aaatgaggat ataaacggaa taaatactga
    13741 gaaaccatca gttctaaaac ggcatctcct aaaaattcaa gtcgctcatt atccgataat
    13801 tgcagattgc gatgctcatt cacataggat gaatgggtaa aagcttgttc aagcaaattt
    13861 agatcgtgaa aaacgatgtt atagttttct tttagttctt ttgtcagctg gttgtccatt
    13921 ttccacattc cttttatgga tattttttcg atacttcctt attatactgg ttttttgtca
    13981 aatttgaaat ggttcggtca aaaggtaagt aaagctttaa taatcctagt gattgacgag
    14041 aagttgtgaa aaattcgttt agtttcgagt atgaagaaaa aattttgtga aaatggcttt
    14101 ccatattttt gcataaattt gacttaatac cgagaaactg gactttgaac accgtttatt
    14161 ggagaagtca tgaaatcagc gccttgacct gaatattaga gaaataaaca gaaaaatatc
    14221 aaaaaaccga aaaaggacat cgcccttttt cggtcatttt tagtgattat gaacgtgaat
    14281 ctgctgtaac agaaacattt gcccatgggt ttggtacatc ataagaccaa tcccagtctt
    14341 tgacacggtc gttgactggc aatacttcat tacggaacaa cgttggaatc gcaaatgctt
    14401 cttcaaatgc atattgttgc catttttgat atgcttcttt ttggtactct tcatcaaatg
    14461 ctttgcttga actgatatcg ttgatcaatt ttgtattttc ttctgattca taacgagtgt
    14521 agttgaacgc agcatttggt ccccatagtc cacttggatt tggatcagtt cctgtattcc
    14581 aagcagcttg gtaaacatca atttcaggat catcgttttc taatttatca tagaaagaat
    14641 tgaattcgat cagacgacct gttgtgtatt ggacatccaa accaatttct ttccattgtt
    14701 ggatatagta atctgccaat ggttgcgctg tttccccacc ggacatcgaa gcaaagttga
    14761 ttgttagttt atttccatct ttgtcttctc ttaaaccatc gtcatccaca tctttgtagc
    14821 cagcatcatc taatagtttt ttcgctttgt ctagatcttg tgtgaatcct ttcatctcat
    14881 catcatggta tgactggaat actggaggaa tcaatgaagt cgcattgctt ctcaaacctg
    14941 catagaaacg ttcacctact gcattattat ctaatgcata gcccattgct tgacgtaatg
    15001 atttatcagc catttttgaa tctggattgt attccacact ctttgtatct gcattccatt
    15061 tacctaactt gaatcctaga taagtataag aagtttgttc acggcctaag tttgtatagc
    15121 cagcaatttc tttataggta tcgtaagtat ctgttggcat ttggaaaatc atatcatata
    15181 atttagagtt caatgcttca gttgcacttg ccatcggtac agatttcatc gtgatctttt
    15241 tcaatttagg cgtaccattc cagtagtgct cgtttggcaa gtattctact gattctcctg
    15301 caacgatatt actcatgtaa taagggccaa aagtcactgg atttttacgt acttgatcgc
    15361 ttgattccat atctttgatt gctacacctt ctaacgtatg ttttggtgct gcatatgacc
    15421 agataccacc acccgattga agcatgcttg ggtttacttc tttatattcg atttcaactg
    15481 ttttgtcgtc tatttttttg attccggaga ttgtgtcggc acttccatca tgatattctt
    15541 ccatacctac gatgttacgg aaagtgtcgt cataacggat accagtgtaa tctttattcc
    15601 caatgatttc gtaagggaaa atcacatcgt ccgccgttac atcttcacca tctgaccatt
    15661 tgacgttgtc ttttactttg attgtcgctt ttttcgtatc ttcatctaga tcaagcgttg
    15721 cagctccgtc gtctgtgatt ctgaaatcgt catcgtttgc aaatagcggt tcatgagaag
    15781 gagccataaa agctgcatca tatgaatctt ggtaaaactc ccactggaac aatcctttaa
    15841 actgactgtc cgttgcgacc gcagcttcta attcgccgtc ttcgattgca tcatcattat
    15901 tttttgttgc aattggaaaa gttaattcct ctttgttatt gccgttgcct ccagagttgt
    15961 ttccagaggt atctgtacct ccgcccccgc atgctgcaag aacgacagca gacatcagcg
    16021 ctgcaaatcc aaaaatcgtt ttcttcattc ctatttcccc cttcgcttct atactagcct
    16081 aaccgttggc gcgcatctgc agaacgcttg aatgcttgtc caacataatt gatactcaac
    16141 atcaaaacta aaataaggat tgatgcgggt aaccagatcc attgccgatt cactaagact
    16201 tctccgttac ttgcataacc gatcaatgta cctaaactag gtacatttgc cggcaagccg
    16261 aaacctaaga aagtcaaagt tgtctcgata ccgatgtttg ccgcaaagtt catcgtcaaa
    16321 ttagtaatga ttaatgaact tagattcggc atgatttcgc ggaacatgat tttaaaatca
    16381 ttggttccca ttgtttttga ggcactgaca taatctcttc ttgcttcaga taatgtcttg
    16441 cttcggaata atcgggcttt agctacccaa taaaatgcac tcatgatgaa tataaaggac
    16501 cagacattgt atctggaaat aatcgatacg aagacgataa tgatcagcat gatcggtaga
    16561 atcatgataa aatcaacgat ccgcatcaag atgttgtcaa tgatccctcc gtagtagcca
    16621 gagatgatcc ctaatccaac cccgatgatc gaagtaagga tcgtgatcgc aaatccgatc
    16681 gtaacagagt ttctagcacc tatgatcaat tgtcctaata catcacgtcc gccttcatct
    16741 gctcccagaa tgaacttttg tccttgtgtc gaaactgttc ctggtgcagc gtatttgtcg
    16801 aagatactaa catacatcac tttgtcttga tcggtcagca tcgcaccgat aaagatcact
    16861 aataataaaa ccactagcaa acctaatgaa aaaatagcaa ttttatcttt tttaaattct
    16921 cgagcaatca ttcgtatccc cataggagga atactttctt gaactgctgt attgtttttt
    16981 tcactcatcg atccattctc ctttctgatt ggttttattg aatccgaata cgcgggtcaa
    17041 cgatactcat gatgatatct gaaagtaatg tccccactag cgtcgcgatt cctaaaatca
    17101 atactaagga tgtgatcacg gtatagtccc gttggccgat ggaatcaata aatagttttc
    17161 cgactcccgg ataaccaaaa atcttttcga taactacaga accagcaatc aacgaagtga
    17221 tttcataccc catttgagaa gccatcggca acgctgcatt tcggaaaata tgtcgactga
    17281 atactttatt cgtcggtacc cctttggaac gggctgttct gacaaaatcg agcgatttcg
    17341 aatcgattac ttcacttcgc agatattgga ttgtgacggc agtcgccaaa aatgcttggg
    17401 taattgatgg taacagaata tatttcaaac ggtctatcca ttgagtgata aatccacctt
    17461 gcaaacctag tgtttgtgat ccacttgttg ggaaccagcc taaacgataa ccaaaaacaa
    17521 atagcatgat caagccgaag ataaataaag gaacagcaaa actaaagaag ttgtagatta
    17581 cgacaatctt atccaagatt gaattttgat accggccagc aagcatcccc agaggcacag
    17641 caatcaaata agtaaggaca actgtcaaga tcgatagata taaagtgttg atgatccgtt
    17701 cgccgatcaa gtcagcaaca gagcgtttgt ataagaaact ttgaccaaaa tcgccttgca
    17761 aagcatttcc gatccaacgg aagtactgga cataccatgg gtcattcaag ccagctgctt
    17821 gtcgcatctg ttcgatcact gccggatcct gatttggatt gatcaaaccg gtaaatggat
    17881 cccccggcat cgcttgggct aataagaaga tcagaacact taaaataata acttgcggga
    17941 tcatcaataa aacacgtcgc agaatcgttt tccacattag cttgcacctc catctttcaa
    18001 cgccactttg tgtgtagttg tgatcgtacg caaatcgtag actcgaccag tcgcatcata
    18061 gtattctttt tggttttgga tatactcttg ttctactctt cgtcgatttt ctttgtggct
    18121 ttccctattt tctacatcga tttgcggaat agctgaaagc agacgtttcg tatagatatg
    18181 tcgcgggtca ttataaatgt cttctcttgt accgatttct acaaatcgac ctttgtacat
    18241 aattgcgata ttgtcgcaca tgtgttttac tacacctaaa tcatgtgaaa tgaataaata
    18301 gctgaggccg tattcttgct ggattcgttt catgaaattc aatacttgcg cttgaacaga
    18361 tagatccaga gcagatacag gttcatcggc tacgatcagt tttggattgg tagcaacagc
    18421 acgggctact cctagacgtt gacgttgtcc gcctgaaaat tcatgaggat atttatatag
    18481 cgcatcttcc ggcatcccaa cgatatctag cagtcctttt acctttttct tttcttcttg
    18541 atcgcttaac cgttcaaaat ttcggatggg ttcagcaatg atatccaaca ctcgcttttt
    18601 aggattcaaa ctggacattg aatcttggaa gatcatctgc acatccttgt tataacccat
    18661 ttttttgcgg ttgcttcgct tcgttacatc ttttccttca tacatgattt gtccggctgt
    18721 taccttttct aatcccacaa cggcttttcc cgtagtagat tttccagagc cagactctcc
    18781 cactaaacca tatgtctttc ctttttcgat aataaaatcc acaccatcta cagcatatac
    18841 atgatccgtt actcgattga agaatccact gcgaataggg taatgaactt tcaggtcttt
    18901 gattgtgatt aattctgtca tctacacctc tcctttttcg tctctaaagt ggaaatgctt
    18961 atagcaggag caacgtacca agtggttcgg tgcgacttca tgaaggatag gctcttcttc
    19021 atgatcgctt gctggtatcc atggaatacg tggtgcaaac cgacaacctt tacgaggcat
    19081 gtttttcaga gaaggaacga caccttcgat cacatggagt tctgcatcat gatttccttc
    19141 ttgtggtata gagttcagta aagagcgtgt ataaggatgc ttaggatgat caaacagttc
    19201 ttttacacct gctacttcaa cgaactgtcc gccatacatc actgccactc gatcagccat
    19261 ttctgctacg actcctaagt catgagtgat caagatgatc cctgtttttg tttcttcctg
    19321 aaggtctttc agtaaatcaa gaatctgtgc ttggatggtt acatctaggg ctgtcgtcgg
    19381 ttcatctgca atgacgattg gaggtttgca ggcgattgcg attgcgatga tgacacgttg
    19441 tctcataccg cctgatagtt catggggata ttggcgtcca actctcgcag gattaggaat
    19501 ccctacttgt tctagcaatt ctagtgcacg agcttgacgc tgttctgcat tcatctttgt
    19561 gtgataggaa agtccttctt tgatctggtc ttcgattctc atgagaggat ttaatgcaga
    19621 caacggatct tggaaaatca ttccgatatc atttccgcgg attttattat agagcgtttc
    19681 gttcaggtcg attaaattca gatcgttata taaaatctca cctgtaattt ttgtgttatt
    19741 aggatcaagt aatcccataa tcgtagtagc caaggtactt ttcccgcacc cagattcccc
    19801 aacgattgct aaaatctcgt ttttgtctaa agtaaatgaa acgtcatcta ctgcatcgta
    19861 atagtcatct ttgagtcgga atcctgtatg taggttctga acttctaata attggttatc
    19921 ggacaatcga atacccctct ctgcttccta aatggaagtt tttcttaata aaaaaggaca
    19981 ttacacagaa taaatcgttt tataacaaag gttattctta tc
//
LOCUS       DACDSY010000042        19821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDSY010000042 DACDSY010000000
VERSION     DACDSY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.042"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWK44_001234"
     CDS             6..305
                     /locus_tag="IWK44_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9549771.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWK44_001235"
     CDS             378..1739
                     /locus_tag="IWK44_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9549772.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWK44_001236"
     CDS             1761..2021
                     /locus_tag="IWK44_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549773.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWK44_001237"
     CDS             2035..2787
                     /locus_tag="IWK44_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP9549774.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWK44_001238"
     CDS             complement(2881..3486)
                     /locus_tag="IWK44_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9549775.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWK44_001239"
     CDS             complement(3539..4513)
                     /locus_tag="IWK44_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP9549776.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWK44_001240"
     CDS             complement(4891..5670)
                     /locus_tag="IWK44_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9549777.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWK44_001241"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWK44_001241"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9549778.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWK44_001242"
     CDS             7161..8558
                     /locus_tag="IWK44_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9549779.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWK44_001243"
     CDS             8639..9079
                     /locus_tag="IWK44_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9549780.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWK44_001244"
     CDS             9227..9553
                     /locus_tag="IWK44_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9549781.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWK44_001245"
     CDS             9554..10303
                     /locus_tag="IWK44_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549782.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IWK44_001246"
     CDS             complement(10383..10838)
                     /locus_tag="IWK44_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9549783.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IWK44_001247"
     CDS             complement(11265..11540)
                     /locus_tag="IWK44_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549784.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IWK44_001248"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IWK44_001248"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP9549785.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IWK44_001249"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IWK44_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9549786.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="IWK44_001250"
     CDS             14770..17436
                     /locus_tag="IWK44_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9549787.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="IWK44_001251"
     CDS             17800..18552
                     /locus_tag="IWK44_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9549788.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="IWK44_001252"
     CDS             complement(18574..19728)
                     /locus_tag="IWK44_001252"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP9549789.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6330 a         3473 c         4060 g         5958 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat t
//
LOCUS       DACDSY010000043        19647 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDSY010000043 DACDSY010000000
VERSION     DACDSY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.043"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..152
                     /locus_tag="IWK44_001253"
     CDS             <1..152
                     /locus_tag="IWK44_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9549790.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="IWK44_001254"
     CDS             complement(230..763)
                     /locus_tag="IWK44_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP9549791.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IWK44_001255"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IWK44_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP9549792.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IWK44_001256"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IWK44_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP9549793.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="IWK44_001257"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="IWK44_001257"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9549794.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="IWK44_001258"
     CDS             complement(4488..5066)
                     /locus_tag="IWK44_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP9549795.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="IWK44_001259"
     CDS             5388..5858
                     /locus_tag="IWK44_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP9549796.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="IWK44_001260"
     CDS             complement(5966..7192)
                     /locus_tag="IWK44_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP9549797.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="IWK44_001261"
     CDS             complement(7946..8146)
                     /locus_tag="IWK44_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9549798.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="IWK44_001262"
     CDS             complement(8280..8888)
                     /locus_tag="IWK44_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP9549799.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="IWK44_001263"
     CDS             complement(8973..9443)
                     /locus_tag="IWK44_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP9549800.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="IWK44_001264"
     CDS             complement(9491..10405)
                     /locus_tag="IWK44_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9549801.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="IWK44_001265"
     CDS             10551..11354
                     /locus_tag="IWK44_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP9549802.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="IWK44_001266"
     CDS             11532..12479
                     /locus_tag="IWK44_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9549803.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="IWK44_001267"
     CDS             complement(12547..12840)
                     /locus_tag="IWK44_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP9549804.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="IWK44_001268"
     CDS             complement(12868..13533)
                     /locus_tag="IWK44_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP9549805.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="IWK44_001269"
     CDS             complement(13673..14305)
                     /locus_tag="IWK44_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9549806.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="IWK44_001270"
     CDS             complement(14312..15379)
                     /locus_tag="IWK44_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9549807.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="IWK44_001271"
     CDS             complement(15376..16107)
                     /locus_tag="IWK44_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP9549808.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="IWK44_001272"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="IWK44_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP9549809.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="IWK44_001273"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="IWK44_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP9549810.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="IWK44_001274"
     CDS             18270..19439
                     /locus_tag="IWK44_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9549811.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         5924 a         3950 c         3142 g         6631 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgac
//
LOCUS       DACDSY010000044        19475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDSY010000044 DACDSY010000000
VERSION     DACDSY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.044"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(21..995)
                     /locus_tag="IWK44_001275"
     CDS             complement(21..995)
                     /locus_tag="IWK44_001275"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9549812.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="IWK44_001276"
     CDS             complement(1075..1950)
                     /locus_tag="IWK44_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP9549813.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="IWK44_001277"
     CDS             2080..3987
                     /locus_tag="IWK44_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP9549814.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="IWK44_001278"
     CDS             complement(4528..7806)
                     /locus_tag="IWK44_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP9549815.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8077..8622)
                     /locus_tag="IWK44_001279"
     CDS             complement(8077..8622)
                     /locus_tag="IWK44_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9549816.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8634..9326)
                     /locus_tag="IWK44_001280"
     CDS             complement(8634..9326)
                     /locus_tag="IWK44_001280"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP9549817.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9345..9629)
                     /locus_tag="IWK44_001281"
     CDS             complement(9345..9629)
                     /locus_tag="IWK44_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549818.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9642..10205)
                     /locus_tag="IWK44_001282"
     CDS             complement(9642..10205)
                     /locus_tag="IWK44_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9549819.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10433..11095
                     /locus_tag="IWK44_001283"
     CDS             10433..11095
                     /locus_tag="IWK44_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP9549820.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11219..12415
                     /locus_tag="IWK44_001284"
     CDS             11219..12415
                     /locus_tag="IWK44_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP9549821.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12516..13892)
                     /locus_tag="IWK44_001285"
     CDS             complement(12516..13892)
                     /locus_tag="IWK44_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9549822.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13929..14795)
                     /locus_tag="IWK44_001286"
                     /pseudo
     CDS             complement(13929..14795)
                     /locus_tag="IWK44_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
     gene            complement(14884..15909)
                     /locus_tag="IWK44_001287"
     CDS             complement(14884..15909)
                     /locus_tag="IWK44_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9549823.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15927..16601)
                     /locus_tag="IWK44_001288"
     CDS             complement(15927..16601)
                     /locus_tag="IWK44_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9549824.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16785..17678)
                     /locus_tag="IWK44_001289"
     CDS             complement(16785..17678)
                     /locus_tag="IWK44_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9549825.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17752..19449)
                     /locus_tag="IWK44_001290"
     CDS             complement(17752..19449)
                     /locus_tag="IWK44_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9549826.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5684 a         4190 c         3444 g         6157 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttttact gtcaataaaa aacaaaatta taataagtgt attttataaa aagaaaaaat
     7921 ataacttaag ttgttgataa taaagagatt tattaacgta aaaagaataa gtttttttaa
     7981 aataacgtat aaaaaagagc ataaaacaaa gtcgtttgtt ttatgctctt cttttattat
     8041 tttgtgcttt ttattttatc aaatcacttt gtttttttat aaaatttctg cgcctagtgt
     8101 ggactcaaaa tgtctcaatg cccattcatg tccaacggga tcgaaacttg ccacggcatc
     8161 tttgaaaata tggagtttgt atcctagatt atacgcatcg actgctgtat gcaacacaca
     8221 gatatctgta catacgccgg tcaaataaat atctgtgatc tggcgttctc gcaaacggat
     8281 gtctagatct gttccactaa aagcggaata atgccgtttg tctatccaat aaacattttc
     8341 ctcttgctca tagtcttggt ataaactttt taaagagccg tacaattccc gtccacccgt
     8401 tcctatcaca ttatgcggag gaaataagcg gttctccgga tgaaaggaat cttttggatc
     8461 atgcgcatca atggcaaaaa cgacaaaatc cttttgatca ataaacttct ttgtatgagc
     8521 agtcagcttt ttttcaatta cttgtcctgc tgtcccagta gtcagctttc catcctctgc
     8581 aacaaaatca tatgtgtagt caatcgagat caatgcgttc atcttagtcc tccttatacg
     8641 agatgtttga caaattcgat caccatttct gctgatcgtt tgcctgcgtc ttcgataaat
     8701 tcgtcaaagc tttgtgtcgc cgagtgatct gctgtgtcgc tcattgcacg tacgatcaaa
     8761 aatgggatat tgaactgacg agctgtttgt ccaacagcgg ctccttccat ttcgcaagct
     8821 aatgcttcag ggaaattcgt taagatttct ttgattttat ctggcgagtc gacaaatgta
     8881 tcacctgtaa cgatcaatcc ttcttttgcg ttcaaattcg ttgcttttgc tgctttaacc
     8941 atttcactgc gcaaatatgt gcttgcttca taatatagag gcattcctgg taattgtcct
     9001 ggtttgtagc caaatcctgt tgcatctgca tcaaaataag ctactttgtc tgatataacg
     9061 atgtcaccta cttgtaagcc ttcaccgatt ccgccagctg aacctgtatt gatcaccatg
     9121 ttcacgccgt attgttggat caacagactt gtggtaatag atgctaagac ttttcctata
     9181 ccggaacgta cgacgatcac ttcgtggtta cctaatgaac ctgaaataaa taaagcgcct
     9241 gcgcgttccc atgataatgg ttcgcttagg ttctctctca aaatttttac ttcttcttcc
     9301 attgcgccga taataccgat tttcatcttt ttaattcctt ctttctatat aaatgcgatt
     9361 gctaaaaaaa ccacacataa cattaggata acaataataa gagatttcat caaaaaagag
     9421 ttccatttgg tcgtcttttc ccgttcgctg atccttgttt tcgtgattgg tttattcttt
     9481 ttgctttctt tacgataaaa gctggcaatc tttttttctt cttgctgata agctgtttct
     9541 gcttttcgct gtttttttaa tgactcgctt gcttgtgctt gttgtcgctt gcgaagttcg
     9601 cttcgcgtaa taagcggtcc tttggccata agccgatccc tctactttcc tttggtcaat
     9661 agttcccagt attgaatcgc ataaatcgtc tttgcatcac aaatcaactg atctttcatc
     9721 gcttgttgcg cctcttcaag agttagatga tacaactcca gtacttcgtc ttcatcttgt
     9781 gctaaaggat tctcgacctt ctctacccct tgtgcatgat aaatatgaag gacttcattt
     9841 gcaaaacctg gtgaaagata catcgaagta agatggctca gcgacttcgc acgatagcca
     9901 gtttcttctt ctaattctct agctgctgtc atttcgggat tttgtccttc tcctggatcg
     9961 atttttccag ctggaatctc caaaatcact ttttctaaag gttttcgaaa ttgtttgaca
    10021 agaagcagac gatcttgttc gtcaaaagca atgatcccga caccaccagg atgaaatacc
    10081 agttctctct tcgaagttcc tccatctggc aaacgaactt cgtctacagc aacatcaata
    10141 atttttccgc tatagatttc tttgcgactg attgtttttt cttcaaattg ctcattgttc
    10201 agcatatctt ctcctgcttt ctttgtaatc ataactgctt ttttttcatt tttcaatttt
    10261 tctcaaaaac tccttattag tttaccatag ataaacctta gattttctct aaatatagaa
    10321 aatccttctt tagtccgata ctctttctag tagtccgtat gctaaaataa gtatgtaatc
    10381 gaataacgaa aagaattcaa tactcaacga taaatgacat ggagggttta ttatgataaa
    10441 acgaaattgg ccgtggcttc tagcattggt cttgctattt ctctttgtcc gaaacaatag
    10501 tttttctttt gttctaggac ttttattgat aggtgtagtt ttagttttgt ggggactctt
    10561 tggttcgcgc aggaaaaagg gaaaacaaga agaaattcct gcactgactg acgaacttga
    10621 aactcactat gaaaaaagcg gtatgacagc gaatgaaatt tcattttttc gacaaacaat
    10681 gaatcaaaca aaaaatgaga tcaatcaatt gcaaatgaat atgcagcaga cagccaaact
    10741 taaagcaatc gatctccgcc atgatccagt caaagcagcg aaagctttat tcaaagaatt
    10801 ggtgaaagaa ccaaaccgac tgcatgaagc aagtcagttt ctttatacgc atctgccgaa
    10861 tctagttgat ttgacaaata agtataatga gatcaatgac catgagatca aaaacaaaca
    10921 gacctatgaa aaactagaag aaagcgcgca gatcatcgac cagctgtcag ctttaatcgc
    10981 acaagattat caaaaattcg ttgctgagga tctagatgat atcgacgtag agatatctgt
    11041 tgcgaaacag agcttaaagc gtgataataa atatgaacca gaacaaaagg attaactctt
    11101 ttgttcataa aagaagactc ggaacattca gatgatgtct ggctgtttcg agtacttttt
    11161 ctaaagtaga gacattcatg taagaatcga gaacagattc aacaaggagg aattttttat
    11221 ggatcataaa ccgactgaaa caaaagatgt tacaccagtt aacgatacac ttgaagattt
    11281 attgaacaac ccgttttcaa ctccaacaga tacgctgacc acttcacaac aagcggagat
    11341 tgatcagctc caagagcaac agacagctgc tcgtttaatt gacaaactac cggcggaacg
    11401 ccaagaacaa gctaaacagc tagcagcaaa aatcgatgct aatgatgcac agtctgtcat
    11461 cagctacgga tcggctgccc aagcaaaatt aagtgaattc tcacaatcga tgttgaatca
    11521 tgtgcaagca caagatattg gtcctgtagg agattcctta actgaactga tgtatcgtct
    11581 ccaagaagcc aatcctgacg aactgcgtgc cggagaaggg aacatcttcc agcgtgtctt
    11641 cgggaaggtc aaacaatcga tctatgaagt gacagcaaaa tatcaaaaga ttggtgcaca
    11701 aattgacaag atttcagtga aattagataa agaaaaagac ggtctgctaa aagataatct
    11761 gatgttagaa cagctttacc agaaaaacaa agactacttt gatgcactga atatttatat
    11821 cgctgctgga gaattgaaaa tggaagaact tcaaacaacg atcattccag aagcgatgaa
    11881 aagagcagaa gaaactggcg atcagatgga tgttcagatt gccaatgatt acacgcaatt
    11941 cctagatcgt ctggataaac gtacgcatga tcttcgctta gctagacaga tcacgatcca
    12001 gcaagcacca caaatccgtt tgatccaaaa cacaaatcag gccttggctg aaaagatcca
    12061 agcatctatc gctacagcca ttccgctgtg gaaaaaccaa gtcgtgatcg cattgacctt
    12121 gcttcgtcaa aaagatgcag taactgctca acgccaagtc tctgaaacaa caaacgactt
    12181 attgaagaaa aattcagaaa tgctgaaaat ctcagccatc gaaacagcga aagaaaatga
    12241 acgtggaatc gtggatatcg agacccttca aaccactcaa aatgatttga tcgaaacaat
    12301 ccaagagaca ttgcgtatcc aaaaagaagg aaaagaaaaa cgtcgtcatg cagaagtaga
    12361 gcttggacac atggaagaag atctgaaaca aaaactgttg gatttaaccc aatagatcac
    12421 ctttcgcttg ttactaaaac aaacgcccaa caacagttta ttacaactgt tgatgggcgt
    12481 ttttcttatc tttcttctaa cttctccatt ggagtctatt tctcattcat cttgctgtgt
    12541 ccatagccat agccaaaata aatgatcagt ccaataacca ttacgatacc gaacacgatc
    12601 catgtcacag tttgcagacg aagcatcaat acaatacaag tcaagatgga gacgattggc
    12661 aatagcggaa cgaatggcac tttgaattct ccagcttttg gtttgccaaa catttttctc
    12721 aacttcagta agcctagact aacaagcatc aaataggcga gcgccatgat attcgttagt
    12781 tccgctaata cattcaatgg gaaaaatccg gcaaagaata cagctgccac accagcggca
    12841 taagtcgctt ttttcggtgt acgatgcttt tgatcgattt ctgtcataaa cttaggaagt
    12901 aagccatcct tgctgacagc aaacagtaaa cgagcaagtg aatacatcat cgagatcgtt
    12961 accgtcagta gcgttaaaat tgcccctaca gcgatcaccg cgcccactgt tccatgtcct
    13021 acatacctca ttgcaaaagc cactgggtca ttgactccta gttcagtata cggaacgatt
    13081 cctgtcaaga tcagcgtcac gatgatgtaa aggacagtag caatcagtat cgaaccaatg
    13141 attcctcttg gcacatcttt ttgcgggttt ttgacttcct cggctgccat tgcaacggca
    13201 tcaaatccca agaaagcaaa aaacacaaga gctgcaccat ctagcacacc ggaaaaacca
    13261 aaaggcatga atggctgcca gttatcaggt ttaacgaaaa atattcctac aatgataaac
    13321 aacgcgatga taccaaattt gacatatacc atggcattat tcaaacgtaa tgctttcttg
    13381 gcttcaagcg acacgatgta agtgacgaat atcactacga gcaccgcaat caagtcaatg
    13441 tatgtaccat tttccggatt gtacccgcca gttaatgctt gtggaagttc ggcccctaaa
    13501 cttttcaaaa aaccttgcat atagccagac catccggaag caacagagga aaccgctagt
    13561 aaaaattccc cgatcaacaa ccaccctgtc agccaggcag caaattcacc aaatacaaca
    13621 tacagatagg catatggccc accaatcact ggcactcgcg aagcaaattc tgcgaaactt
    13681 agtccagaca aaatgacaac gattgcagct aagacaaatg aaagtgacag tgctggacca
    13741 gcattttgat tggccgcaac ccctgtcacc acaaatattc ccgtaccgat gatcgcacca
    13801 attccaagca tgatcaaatc agacgttttt agttcttttt tcatctcact cgtatgattg
    13861 agattgatct ctttcttacg aaacaaatcc attgaagttc ctccccattt taggttggaa
    13921 aacagcgatt aaactgtttt catataccct aaaaggatac cacctaaaat gactaaaagg
    13981 caaccaacaa taacataaat gagctctttt ttcgtttttc tttcgccaag gatgaaaatc
    14041 ccacctaaag tagagataat gatccccatt tgtgaaagtg agaagccaac agctaaacca
    14101 agtgatttga ctgttagaag catgcagatg tttcctaagc cccataataa tccacagatc
    14161 atgtttttcc acacaaactg atcgacttta actttacgga aagcaaagaa gcttgcgcct
    14221 aagatcattc cgatactttg aggtaagata attgacatcg catccaaatt cgcccagttg
    14281 acgatgatgg tatatacacc ataaccaatc gtagagaaga tcaatgctct aaaaccctta
    14341 ccaaaatcaa gcattttatt atctgtatct atttttcctt cgtcatcttg tcgggcagtc
    14401 aaatacgcac cgcttacaag aagagctaaa gcaataatac cctatacata atctttcgtt
    14461 tgtgtccatt catggaagaa aacggctccc gcaatcgtat tcaaaattaa ttgaaaacca
    14521 gtagacaatg gtagccctac agaaacaccc atgtacttca ttccatggaa ctgtccattt
    14581 tgtccaacac tccaagctaa gccggataaa aatccgatca agaaaatcca aggggtgatt
    14641 acaggactaa caacgaaaaa gacaactaaa gaaaacagaa atgcaccgat cgtcattcct
    14701 agtgtttgtt gattggcatc gccaccaatt ttaccactta ctaaaccaat actaccccat
    14761 gccaacatag ggactaatgc tattaaaatt tccataacca atctcctttt atttcacatg
    14821 ataagaaact tatcagcgaa aacgttaaca acacaagcac tttttttcgt ttctaacgat
    14881 tttctaacgt ttttcattat aaaaatttaa gattgggaaa aataaagtca ttgttgtccc
    14941 ttttccttca gttgattcag ccgtcaaatg gatacctaat ttttcggcca gattttttgc
    15001 taaataaagt cctaatcctg ttgaatgtgt tttgctaagt cgaccatttc tcccagtaaa
    15061 tcccttatca aagatccgtt tcaagtcttc cgctgggata ccgatccctg aatctttaac
    15121 tgaaaggctg actccttgcg cttccttttc tatgctcaca atgatgtttc cgtgatccgg
    15181 cgtatatttg atggcattac taagcaattg gttgaagata aaagcgatcc atttagcatc
    15241 ggtcaaaaca gtatgatcct caccaacgat ctcgtagcga atattttttt ggataaaata
    15301 atttgcctga ctgcgtatca caggctgaat gattcctttt agtccatatt cttggatgag
    15361 gtagtcccgc gagaaactgt cgagtcttgc ataatacaaa acttgttcta cgtattcatc
    15421 gatttttgat aattcatttt ctagtaatgt atacttttga tcgtcaatat cgaactcgat
    15481 cgaacgaact agcaatttag aagcggaaag tggcactttg atttcatgga cccatgagtc
    15541 aatataatct ttttggtctt gttgattact gattgctgat tgcattactt cttggtgttc
    15601 acggatcagc tgattgatat attcttctac cagtttttct tcttctgtcc tcccaccact
    15661 caaataattt tgcaaagggg aagttttttg tatgtcggct aattttcccc accatcttct
    15721 cttgctcaag taaccaccca gcagataaaa aatcagaaag acgccctcaa tcaaagctaa
    15781 ataagcaatt gtgctcaaat caactttgat gtttggcgcc agccacatca caaagatcgt
    15841 caatgcaata aaaaacagcc atcccaagat caatgtccac tgatctttca aatatttaaa
    15901 aaaattcatc ttaaccgctc atcctttcag ggaacgatat atccctgtcc tacttttgtt
    15961 tcgatatatc cttggatacc tgcctgttcg atttttcggc gtaaccgatt gatgttgact
    16021 gtcaatgtat tgtcatctac aaatcgttcg tcatcccaca gtgctcgcag caatttttcc
    16081 cgacttaaga tctttccatt gtgcttcatc aaaataaata atagacgata ttcatttttg
    16141 ctcaaatcaa tgatctctcc gttgatttcc atacttccgt tgtcaacatt caacgtaatg
    16201 ccattgtggc tcatcatttc actagataat tctgtgtagt tgtaagaacg gcgaagtaat
    16261 gcattgattt ttgcaatcag tacatcgatt tggaaatgtt ttgtcacaaa atcatctgcc
    16321 cccatgttca tagccatgat catatccata ttcgtattcc tgctggagat aaaaatgatc
    16381 ggtactttcg atacttcacg tattttttga caccaataat agccatccaa aacgggaaga
    16441 ttaatatcta gtaatacaag ctgtggttct tctctttcaa agtctgagaa cacttgattg
    16501 aaatcggtcg ttccaaaggt ttcaaattgc catttctcaa gttcctccat gatcaattgt
    16561 cgaatgatct cttcgtcttc gactaccatg atttttgcca taaaaaatcc ctccatcttt
    16621 cttcttgcta ctacaatatc aaagaagccc ttgtgaaaca agttggctta cctggaaaat
    16681 gtcattttag caaacgttaa aaaaaactgg aaatatgcga tttaatcgcg tttgtctctt
    16741 ccaagcagct taaatttatt atgttattct taataattgg tctattatct gttatttttc
    16801 tttcgttgtt tttgtctttt aaattttcga taacaccaat aaccgaaaag cgttagcact
    16861 agcaatatac ctagaaggag tataagtaat cgataaagat ggtatttttt tgtctgttct
    16921 atctttgaag agacttcttc tgtaaaggga actcgtcttc ctgtcactag taatcggtga
    16981 gtgttcacgg tgtaaggtgt acaagtgata agtgtgacta gatcctgctt ttctctaatt
    17041 gctaatgtat ttgtttttgt tggaaggaca acaattttct caacgacttc ataagcgagc
    17101 atccgaccat acacttcaag atagaatcgg tcatcttgct ccatttcttc aagatccgta
    17161 aagagttttt tctcagcaat accgctatgg gcggtaatca cggaatgtgt actctctcct
    17221 ccagttggaa aagaggtgcc ttgcaggact gtcgctcctc tatctaacaa aagatcattc
    17281 gtttcatcaa ataaaggcaa gctaacattg attttcggaa tatagatggc tccgatcata
    17341 tgttgttcgt aatattcttt tcctggattt tggacatttc taaccgcttg ctcaaaagga
    17401 tctttgactt gtcccattcc tgggattact gatgtatgag caagcgtctg atttttcttt
    17461 tcatgcactg ctaattgttg ctcatgtttt tcttctttat cgcgttcaag ctgcttttga
    17521 taattctcga tccttctttg atccaaataa ttgctgagag catctgctaa aaatggatat
    17581 gtaaaaactg cagctccggc aagaaacatc actgccataa ataattgtaa taaaagactg
    17641 atttgggttt gaggtctatt acctattttc tttttcattt tcttcacctt cttatttaaa
    17701 aaaactgcag caacctttat aaaaccgatt gctgcagctt atttttgttg cttatacttc
    17761 agacttcatt ttagatttac ggtaccagct gtacgctcca atcatcaaac ttattccgat
    17821 tagtaagaat gccaagagcc cgttcccacc agtagcagga agtgacccgc gtttcatatt
    17881 cggtactctt tctggccctg gattttctac tcctacgatc tgaatttctt cattatagct
    17941 tccataagag atgataaatt cagtaaaggc tgcctcacct gttggaagta cataaccttc
    18001 tggcgccatg gtctctttca tttgataggt tccataatct aacccttgaa cctcaaagta
    18061 tccatttcca tctgcgacaa attctgttgc ttgtccgctt gttgcccact caattctatc
    18121 tgctcctgat tcataaacag ctaaaggcga ctccccttta taaaatattg cgtattcaga
    18181 acggtcttca ttccacaatt cgaatcttgc atctttaagc aattcactgc ttgatgcatc
    18241 atatttttca aactttcttc cacctgttgt gactttctct ggcggctcta ctccttcttc
    18301 ataatcaaat tcatcatttc gtccgatttc aataaccgct gtgttgttat ggaaatcatc
    18361 cgcttctagt tctgtggaaa ccttcaaatt atacgtgatc gttaaatctc ctccacgtgc
    18421 atctgctaat ctacctaaag tatctacttt ggctactcca ttttcttctg tcaaaatcgt
    18481 cacgacaaac ccgttgctta ctacttctac tatataatct tgtccttttg ttaaaatcgt
    18541 ctcgccggct cgcacttcta tttcctgatc ataataagtt agaccaactg taggcgtatc
    18601 tgtgattttg taatttttga taacagcgct tccgttttca aggacagaat cgatgaaata
    18661 aggaataggt gctgtgatcg tgtaagaagc cgtttttcct ctttctagat caatataaga
    18721 aatcgtttct tgttctccag cttcattaat aatcgttact tgtctaaggt cgctttcctc
    18781 tacattcatc tgtttatctg cttctctgat ttcgtttttc ggatacaaat gcacatctgt
    18841 attgtatgtg tctttccatg tagaaccatc ccacacgcca tccaccatat caggcatcat
    18901 cactggcatc ataacaacca ttggagacgc gattctggaa atatgagctg gcgaataagt
    18961 ttctaagaaa agatagactt tatctctaga agaaggtgct tcttccgtca cttggacggt
    19021 agagaatacc gcttctccag cttcattcgt tgtttgtttg tcaatacttg tcagtcgata
    19081 ttttaaaaac caactttctg tgtgccgttt ttgaatccat tcaatcgctt cggcagaatt
    19141 aagaccgtct tctcgttccg gtgttaaagg attatctcga atcaaatcgt aatatacttc
    19201 cgttacgtca aacaaatcaa aatcgactcc tggaatattt tcttcaccaa aatccgtaga
    19261 aaccaatccg ctattcggtc gttcttccgg aacagatgag aaaccttttt tgtgtaatgt
    19321 aatcgtcact tcacttggtt tttgtgcgac ttcttcggct ttgactagtt ttccgttccc
    19381 gaaaatccct attcccgcag taacgagtaa cagtacactc attcccgtga caatcatttt
    19441 tttattcatt tttttctccc cgttcttttt ttatt
//
LOCUS       DACDSY010000045        19390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDSY010000045 DACDSY010000000
VERSION     DACDSY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.045"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWK44_001291"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWK44_001291"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP9549827.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWK44_001292"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWK44_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP9549828.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWK44_001293"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWK44_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP9549829.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWK44_001294"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWK44_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP9549830.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWK44_001295"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWK44_001295"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP9549831.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWK44_001296"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWK44_001296"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP9549832.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IWK44_001297"
     CDS             complement(8053..8523)
                     /locus_tag="IWK44_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP9549833.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IWK44_001298"
     CDS             complement(8569..8994)
                     /locus_tag="IWK44_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP9549834.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IWK44_001299"
     CDS             complement(9061..9330)
                     /locus_tag="IWK44_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP9549835.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IWK44_001300"
     CDS             complement(9341..10936)
                     /locus_tag="IWK44_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9549836.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWK44_001301"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWK44_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP9549837.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IWK44_001302"
     CDS             complement(14489..15049)
                     /locus_tag="IWK44_001302"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP9549838.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IWK44_001303"
     CDS             15402..16376
                     /locus_tag="IWK44_001303"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9549839.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IWK44_001304"
     CDS             16546..17946
                     /locus_tag="IWK44_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP9549840.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IWK44_001305"
     CDS             complement(18024..18746)
                     /locus_tag="IWK44_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9549841.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IWK44_001306"
     CDS             complement(18825..19331)
                     /locus_tag="IWK44_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9549842.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
BASE COUNT         5285 a         4318 c         3471 g         6316 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag
//
LOCUS       DACDSY010000046        19376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDSY010000046 DACDSY010000000
VERSION     DACDSY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.046"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..506
                     /locus_tag="IWK44_001307"
     CDS             <1..506
                     /locus_tag="IWK44_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9549843.1"
                     /translation="IDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVET
                     IERALVNQAKWQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGL
                     KKIRFHDLRHSNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALE
                     NAFGFKE"
     gene            1080..1649
                     /locus_tag="IWK44_001308"
     CDS             1080..1649
                     /locus_tag="IWK44_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP9549844.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1636..2496
                     /locus_tag="IWK44_001309"
     CDS             1636..2496
                     /locus_tag="IWK44_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9549845.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2686..3390
                     /locus_tag="IWK44_001310"
     CDS             2686..3390
                     /locus_tag="IWK44_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9549846.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3395..5230
                     /gene="walK"
                     /locus_tag="IWK44_001311"
     CDS             3395..5230
                     /gene="walK"
                     /locus_tag="IWK44_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP9549847.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5227..6543
                     /locus_tag="IWK44_001312"
     CDS             5227..6543
                     /locus_tag="IWK44_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549848.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6544..7413
                     /locus_tag="IWK44_001313"
     CDS             6544..7413
                     /locus_tag="IWK44_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549849.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7468..8277
                     /locus_tag="IWK44_001314"
     CDS             7468..8277
                     /locus_tag="IWK44_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9549850.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8380..9009)
                     /locus_tag="IWK44_001315"
     CDS             complement(8380..9009)
                     /locus_tag="IWK44_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP9549851.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9179..10471
                     /locus_tag="IWK44_001316"
     CDS             9179..10471
                     /locus_tag="IWK44_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9549852.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10547..11209)
                     /locus_tag="IWK44_001317"
     CDS             complement(10547..11209)
                     /locus_tag="IWK44_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9549853.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11434..11718
                     /locus_tag="IWK44_001318"
     CDS             11434..11718
                     /locus_tag="IWK44_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP9549854.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11769..13394
                     /gene="groL"
                     /locus_tag="IWK44_001319"
     CDS             11769..13394
                     /gene="groL"
                     /locus_tag="IWK44_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP9549855.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(13483..14631)
                     /locus_tag="IWK44_001320"
     CDS             complement(13483..14631)
                     /locus_tag="IWK44_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9549856.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(14733..15317)
                     /locus_tag="IWK44_001321"
     CDS             complement(14733..15317)
                     /locus_tag="IWK44_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP9549857.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(15444..15875)
                     /locus_tag="IWK44_001322"
     CDS             complement(15444..15875)
                     /locus_tag="IWK44_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9549858.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(15893..16213)
                     /locus_tag="IWK44_001323"
     CDS             complement(15893..16213)
                     /locus_tag="IWK44_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9549859.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            16951..17208
                     /locus_tag="IWK44_001324"
     CDS             16951..17208
                     /locus_tag="IWK44_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAP9549860.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(17179..17487)
                     /locus_tag="IWK44_001325"
     CDS             complement(17179..17487)
                     /locus_tag="IWK44_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549861.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            17565..18311
                     /locus_tag="IWK44_001326"
     CDS             17565..18311
                     /locus_tag="IWK44_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP9549862.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            18326..18529
                     /locus_tag="IWK44_001327"
     CDS             18326..18529
                     /locus_tag="IWK44_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9549863.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            18634..18933
                     /locus_tag="IWK44_001328"
     CDS             18634..18933
                     /locus_tag="IWK44_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549864.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            18933..19115
                     /locus_tag="IWK44_001329"
     CDS             18933..19115
                     /locus_tag="IWK44_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549865.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(19121..>19376)
                     /locus_tag="IWK44_001330"
     CDS             complement(19121..>19376)
                     /locus_tag="IWK44_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549866.1"
                     /translation="TCWTMLNKNQRDVENIAIFLIKITSSISIIIPMTVTFSKEQFNV
                     AATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
BASE COUNT         6448 a         3304 c         3904 g         5720 t
ORIGIN      
        1 ctattgattt tttaaacaaa aaaatcatga taaaccatgt aatcatagaa aatccagaga
       61 atatgtcaca gttaataaag aaagatcaaa caaagaactc ttccagctat cgtacattac
      121 ctttggttga aacgattgaa cgagcattag taaatcaagc taaatggcaa caagataatc
      181 gtattctttt aggagatgat tatattttag aagataatga gtacgtattt actatggaag
      241 atggacggtt gatgaagcct caatatgtaa cgcatcgttt gagtaagttg attaagaaaa
      301 atggattgaa aaaaattcgc tttcatgact tgcgtcattc taatgcttca ataatgttag
      361 atagtggcca gaatatgaaa tccattcaag aatggctggg acatgcatcg tattcgacaa
      421 ctgctaattt atacacgcat ttaaccgcag gagtaaaaga tcgtgcagca gaagcattag
      481 aaaacgcatt tgggtttaaa gaataaaaaa acaaaaaaca atatctttgg ggtatcgctt
      541 cttgttttta gaattttttt gttggcaatt tgttggcagt atgttggcaa tgaaaaaatt
      601 cacaaactaa aaacctattt ataaagcttt ataaaacttt tttcacaaat agagcatttg
      661 gttaagagtc agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat
      721 agtagcacta agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta
      781 cttccttata ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac
      841 caacaaacaa agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt
      901 tcccgccgtc tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc
      961 ttcttgtttt ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga
     1021 ttgaatgttt atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga
     1081 tggaaaaaat gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc
     1141 ctcactggga agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg
     1201 atcgttatct ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg
     1261 taaacaatta cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag
     1321 aacacgtagc atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa
     1381 tcaaagaagg gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat
     1441 tttgcgatga acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc
     1501 atccgcaaaa attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt
     1561 tatcgtttgc aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa
     1621 aagaggagaa aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat
     1681 gttccagaag aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat
     1741 aaagatcagg tatatacaga taaaattgac attacccctg aagagatcta tcaacgtttg
     1801 ccagaggaaa tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag
     1861 caaatcaaaa aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc
     1921 gggacatata atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta
     1981 ctagatacga aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg
     2041 ctggaagaag gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga
     2101 acaaaagttt tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt
     2161 ttagttgctt ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa
     2221 gaagggattt attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg
     2281 gttgcttacg tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat
     2341 ggggatgcga aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa
     2401 gcaaaccaaa tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga
     2461 ttattgggtg tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt
     2521 gaaaatcagt gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca
     2581 ctttcatttt aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg
     2641 gtataatgag aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta
     2701 gttgtcgatg atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc
     2761 tatgacgttt atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa
     2821 cctgatttga ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt
     2881 gaggtacgca aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt
     2941 gataaagtcc taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac
     3001 cgagaattag ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa
     3061 ccagaagagg cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct
     3121 tacatggtga cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc
     3181 ttttacttgg cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt
     3241 tggggttatg attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt
     3301 gaaaagatcg aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt
     3361 tactatttgc gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg
     3421 atcagtcaat tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat
     3481 tattggtgcc tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga
     3541 tatgaatcaa acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga
     3601 taatgcagat gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac
     3661 tagcgatatc atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga
     3721 tgtcaatgaa caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac
     3781 gactaaacgt tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc
     3841 gatcctgtcc ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga
     3901 acaaaaatat tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc
     3961 ggcatttatt tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg
     4021 tgaaatgaga gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt
     4081 gcatggacaa gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat
     4141 tgaagaagct caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca
     4201 tatgacagac ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat
     4261 ggctgtttcc ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact
     4321 tttagatatc gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt
     4381 acttgaccgt tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc
     4441 tatgatccgt cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac
     4501 agaacaagaa aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact
     4561 tcgtacaccg ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa
     4621 agacgaggaa gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat
     4681 tcgaatgatc aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca
     4741 attagaatat gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat
     4801 ggtgggaaat caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg
     4861 ggtcgaagta gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat
     4921 caagtattca ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt
     4981 gattttaagt atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt
     5041 cgagcggttc tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg
     5101 attagctatt tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa
     5161 ggaaggaaaa gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg
     5221 gtgggaatga gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta
     5281 agcatatact tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta
     5341 gatgagacaa acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt
     5401 cttccgcttc atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat
     5461 ctcgtgtacc gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta
     5521 gtttcaggag acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg
     5581 gcctataatg cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat
     5641 gtgtcgaact tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa
     5701 ggaaataaaa aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt
     5761 tcgttgaatt ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa
     5821 atgacaacag aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg
     5881 aagaagtata gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc
     5941 cagaaaccag atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg
     6001 aatgaaacaa tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca
     6061 gagaaaaaag aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc
     6121 agttctttag ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata
     6181 ttcgtggaag gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt
     6241 ggcggtgaaa cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa
     6301 gtgccgatac cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag
     6361 ttaaaagcta agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg
     6421 caaaatgtca aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat
     6481 aaagataact ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat
     6541 taattggatt tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg
     6601 tttttattca gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat
     6661 cagactgaat caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt
     6721 tcgtctgaga acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca
     6781 ttagcgaatt atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac
     6841 ggtgatgttt tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca
     6901 gataaaatta gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt
     6961 aatgattatg tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa
     7021 gcaagccaag agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct
     7081 ttagaaaaaa acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat
     7141 atcgagcagc tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg
     7201 tacatcaata acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc
     7261 tgtattctga aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa
     7321 acaagcgata aagtggtcca aattgagagc gtcaacgctt tcagcaatac gatcgtgaca
     7381 aacaatactg taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta
     7441 caaagtaaga agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt
     7501 atcctagcaa gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt
     7561 ttggtcgatg caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg
     7621 acaccggacc aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc
     7681 gtgggtgtct tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa
     7741 gcgatggaac cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg
     7801 aaagtcagaa cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg
     7861 gctccgcaat tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc
     7921 ggatattgca gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa
     7981 agtaatcatg atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt
     8041 attttaggag atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg
     8101 atcggtaatc ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa
     8161 gaacttgctc atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat
     8221 gattttgaaa tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca
     8281 gttcaatcaa taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt
     8341 ctatttttat ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat
     8401 gtgctgcttt tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc
     8461 catgctgatc gtactgtcgc tagcagcgtt tctttgtaaa taatcatcac ttaacaaata
     8521 agtacctgta cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg
     8581 tagattttta gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata
     8641 gaagggtgca tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa
     8701 taacgaagag gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat
     8761 caaatgggac aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc
     8821 aacgacaaca tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat
     8881 aaacggaaca tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc
     8941 tctggacaat gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc
     9001 aattttcata aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc
     9061 gtcaaagaat ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt
     9121 gggtatagtt aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat
     9181 gaaagagtgg ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc
     9241 cttaactgct ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct
     9301 gtttttctta tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg
     9361 attttctatg tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat
     9421 cgtttttgcc attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg
     9481 cataacagcc ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac
     9541 tgtctggaca ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg
     9601 gggctatgca cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga
     9661 aaatgtttgg cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct
     9721 tatcggttca gcggtttttc aacatttgtt cagaatgact gttgttcaag gagtatggac
     9781 atatgtccag cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg
     9841 tttttttgtt ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt
     9901 ggttagtccc gtcttgcaag cgaattcatt agcaaatgca gaaatcttag cctcaggaca
     9961 agagttgacg gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat
    10021 gacggttacg ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa
    10081 atcgaagaaa tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa
    10141 tgaatcaatt attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat
    10201 tttcgcaccg gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc
    10261 tttatttaca gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt
    10321 aggcggagtg cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta
    10381 tttcccattt ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa
    10441 tgatggagca gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat
    10501 tgggaaaatc accaatctag gatgacagga gtatttatag tcatgtttat actagccctt
    10561 tttggacagc taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg
    10621 atgtccagat ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt
    10681 agattagcag atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc
    10741 cgacccaaaa atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact
    10801 cttccatgat gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca
    10861 tctggatgat attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta
    10921 acatgacagc gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa
    10981 caatctttgg agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct
    11041 taaaataaaa aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga
    11101 tcaattcaga cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa
    11161 ttagaccata gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc
    11221 ttttaaggga taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata
    11281 atttcaataa gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa
    11341 agagggagaa tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac
    11401 agattaaaaa aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc
    11461 attatcgaag tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct
    11521 gctaaagaga aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa
    11581 aacggtgaaa aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac
    11641 gccggtacag aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc
    11701 atggcgattg ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg
    11761 aatcaatcat ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg
    11821 gggtagataa attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg
    11881 tactagaaaa atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag
    11941 aaatcgaatt agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat
    12001 caaaaacaaa tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa
    12061 tcgttcgcga aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg
    12121 ggatcgaatt ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg
    12181 attcaaaaga agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac
    12241 atttgattgc tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat
    12301 caaaagggat cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact
    12361 tatcacaata tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata
    12421 tcttgatcac agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa
    12481 tcttgcaaca atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc
    12541 caactcttgt attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg
    12601 gtttcggcga tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag
    12661 taattactga cgatctaggt ttagaattga aagatgcaac aatcgaaaac ttaggaaatg
    12721 cttctaaagt tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag
    12781 aagcaatcga agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt
    12841 ttgaccgcga aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta
    12901 aagttggtgc agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat
    12961 tgaatgctac acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag
    13021 tgaacgttat cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca
    13081 agatcgttgt tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg
    13141 aaggatcagt gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg
    13201 ctactggaga atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac
    13261 gttctgcctt gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg
    13321 ttgcagataa accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg
    13381 gcggtatgat gtagtaatta agaaagaacc ctattttaac agggttcttt cttctttttt
    13441 tgttttattt taagcaaaag gggcaaagaa ggggcaaaaa tatcaaaggc tatttagttt
    13501 atcaattact tgttgtttgg ctctttttgt tacatggtta taaatagaca gagttgtgtt
    13561 tgcatcagaa tgccctacac gctccataat ggctttaaga ggtacgccta attcagataa
    13621 tagtgaaacg tggctatgtc tgaatatatg tgaggataga cttttttcta attccaactc
    13681 ttcctctact ttatggagta tagcgttaaa tgagtgcagc gcaagcggag tgccacttgt
    13741 agatataaat atatattgat cgggatctgt gggtctacct gcaagaatat tgtcagctat
    13801 cacactttca atcaattctt ttgcacgatt gggtaattgc acttcacgtt gcgaataagt
    13861 attttttgga gttgttttta tagcattatc cattttcaca gatgtgtaat ctaaggtccc
    13921 attaatggaa attttcccat cctcatagtc cttcatttgc aaagctagca attccccata
    13981 tctcaaacca gttaaatata gaaactcagc tattatgccg tgtagttttc tgcgaggatt
    14041 ggagtatagc tgtttcagta tttgatcaat ttcttctttc tccagatatt ttttattcat
    14101 agaaagcctt cttttttctt cttccacttt tttaggatgg attttaactg ctagcgcagg
    14161 gtttctttga atgtattttc tatcgattgc atagtttagc ataacagata gagttgtttt
    14221 tgtttgtttt gtgtagttca gtgagaggtc accaaacgta tacatatctt caattatctt
    14281 attaatgagt gtctcatcaa tgtttctaac gatcgtatca tcgcttatgt gcttagaaac
    14341 atgtttcatc atcattggaa ccttcaaata gctagtacgt ttaacatgct gcttataata
    14401 ttcataccat tctttataca gctcaccaaa agtgatatct gatttattgt aatcttcgag
    14461 tgcttcttta atttttttat ctagaatttt ctgagctttt ttccacgctt gtggtgaatt
    14521 acttgtaagt gttgtagatt tttttcgtgt tttttctgta taaggatcta catatctttc
    14581 aataaacttg aattggccat ctttggtctg ttcaacccac acttttaaca tctcctatca
    14641 tttgctataa taggcataac aaatagacct atataggttt gttttctaaa agcacgctct
    14701 tactctggac ggtggggcgt gttttttatt tattatacag ttgctttaaa aatagctgct
    14761 ttttcaaatg atactaaagg agagaagtgg atttctattt ctcccttttg gtttaacgtg
    14821 aaatgagcag ttacatccat ttgttttcct ggagcaacag atcccattgt attttcgttg
    14881 gcatatgtct ctgatttttt gtcatctggt ccatatactt ccacatctgt acctacaggg
    14941 atatctgaat cgccatcatt ttttacggta taagtaattt ttactacttg tgcaggttgg
    15001 ttttcttcaa attgatttct ttcatcagtt agttctacac tatttagtgt atattcagca
    15061 tcaccaactt ttacagtgtc accaatctta tagaaggtat cgcttttttc ttctttagaa
    15121 gatgaagcag aggttgaaga tttagttact ttttcgccac cattatcact tgctttatta
    15181 ctatctgatc caccatttaa agcagaacca ataattataa ttaaaattac tgctaatacc
    15241 caaaaccata cttttttgta aaaaggttta ctaactttat acatttttcc gtcttgaccc
    15301 ataacttttt ttgccattta aatattcctc acttcttgtt ataatatatt tgcgatctca
    15361 gaaatgaggt atgagtccgt gtgccagcac gggctttttt aatgttttgc tgaaatcggt
    15421 ttttttaata attcttgatg tattcataca tattgccttg cgtaagaata ttttttctta
    15481 aaatagcatt ggcagacagc ataacaagtg tatctgagct tattctgaat agaataatac
    15541 tccataaatt tttcgagatt aaactgagat tcatcagtca gttcgttctc aatatagata
    15601 tttaataaaa ttagaatagc tattttatct gcttctgttt caaattttga gtgaaaagtt
    15661 gtagaagtat cgtacaaaac tgaatattca aaatgagaag cggtgaaatg cgcaagttca
    15721 tgagataaat gaaaggcttc tgcagtttcg ccatatagat tttcattcaa aaaaatgatt
    15781 ctaggcttcg gataatagaa gccaggctct ttcatttcca tgtaaactac tttcaaattg
    15841 tattcgctca acatttcttt caatttcaaa tacatacaac ccatcactcc aactattcat
    15901 tttcctctaa agctttagca attgcaatcg ctttacgcat tgtctcctta gatatttctt
    15961 ttccatcaaa agaaaaaaca gtatcgtttt ctgataaatc cacatgttta ggggtttctc
    16021 tttcttctct acctagaagg tagtctacag agactccaaa gtaatcagct aaccgagcta
    16081 actcaataga attaggagtt tgtcttttcc aacttcctaa atatccattc gaatacccga
    16141 aattcatttc gagctgtcta atggataatc ctttttcttt tgctaactcc tttattattt
    16201 cgtaagtatt cattgataaa tcaacctttc tgaatgctta caaaaaaagt ttagataaat
    16261 aatgtaaaaa tagttgactt atatacatta ttaatctata ctatgttttg taaacaagtt
    16321 taacaactaa aaagacaaca aaaaatacta ttgattaata aatgccaacc gccaagaaag
    16381 ctttttaaat caaagtttat atgtcttatt taactatgca tttagtatag attattaatc
    16441 taataaagtc aacgcagttt tttaaaaagt tgttaaattt gtttacgaat ataaaagaaa
    16501 ggagagaaga atatggaaaa aacagtctcg gcaaaaatcg aagatttgaa attagacata
    16561 ctgaaacaag caaaagtggc gatggaacac gcggtagata aagaagactc tgccatggtt
    16621 gcagccatag cagagatttt agctcacgtt tagtcatcgt ttttacggtc ttctggaagc
    16681 tgaccataga taacagcata atgaatatct aaataagctt gaacaatttc ttttggcgaa
    16741 attgattcgc ctttagtaga tacttgtgat tcgtgattta gccatgcaac aactaaatcc
    16801 gcagcgattt caaacggcgt tacactttgt gtcagttgcc ataaagaatt tcacaatatg
    16861 tatggtcgtg gtaataatac caaagagcaa tttgaggaat ttatggaaat taaaaaatac
    16921 aaaaaatcat ttattaaagg aggctaaaca atgaacaagg aaatgagcca aacaatcaag
    16981 gtacaaaaaa tgatagatga tctaacacat ggaattgata gccaagccga taaaatcatc
    17041 aaggaattgc aaggacaaaa agttaaggat gcaaaaatgc ttctcaagac tatcaacttt
    17101 gaaatgaatc caacaaaaag aaagctcgcc gatgtgttgg aagaaaagtt ggcttccgct
    17161 atcaacgagc aagaattact atttgaaacc gatactttta acagctgatg ttttatagat
    17221 gacagtttca tccgaatcta tatcatcgcc gagagaaaag aatgttgctt ttgacaaaaa
    17281 gccgataaga cctacaagcg gtcttgatgt tgcaagagta gataccccaa tatcaccttg
    17341 caaggtattc acgtaaattt gagaagaata gaacattcca tctttttctt cgatatttga
    17401 cgcatcatcg tatccaacga ccaaagtatc ggcaaatatt ttgatttttt ctccatcaag
    17461 catagtaatt acaaattctg gtttcataaa ttttcacctc cttaacaatt atttcagcct
    17521 gtcacactga taaggaaatt ataccaaaga aaggaatgag aaatatgaat acaccacaaa
    17581 tttttaattt cgagcaaaac gaagttcgaa ctattttagt aaatgatgaa ccatattttg
    17641 taggaaaaga tgtagcagac gttctaggat attcgaatcc gcaaaaagct attcgagatc
    17701 atgtggactt ggaggataag acgcagaacg attcgttcac cgtcaatgga acagcagttg
    17761 ttttgattaa cgagtcaggc ctttacagtt taatcttaaa atcaaaactt ccctctgcca
    17821 aaaaatttaa acgttgggta acgagtgaag tccttccaac aattagaaaa acaggtagct
    17881 attcaaacgt acctcaaagt tttgcacaag cattgcgttt agcagcggat ttagaagaaa
    17941 agaaccaatt actcgaacaa caaattgccg agtacgaacc aaagattagc tacttagata
    18001 cgattctttc atcgacagat acggtagcta cttctcaaat tgcagctgat tacggaatgt
    18061 cggcaattgc tctaaacaaa ttgctaaacg agttaggtgt tcagcataaa gttagcggac
    18121 aatggatact ttaccgaaaa catatgaacc aaggatacac aaaatcgcac acaagtgaga
    18181 taccgaaagc cgatggcggc actaaagttg taatgaatac caaatggaca cagaaagggc
    18241 gagtgtttat ttacaactta ttaatcgcag agggctatta ccctcaaatg gatttagagg
    18301 aaattggtta gaaaggagtt ttagtatgac tgacattgca gaaatcactc aacgagatag
    18361 agaaaaaatc aaagaatatg tcgaaagttc aaagttctta acttacacca tgcttgctga
    18421 aagatttgga attagcaaaa gctacttatc tttaatttta aacggtaaaa agacttctgc
    18481 agaagcaaac agaattatag attcgattat cactatgtac gaattgtaga gggaggtaaa
    18541 gtcgttggaa gataaaatca ttcttacaaa atcagagctt caagaaatgt taaacatcga
    18601 gtatggaacg ataattttta ggagggataa taagtgaaaa aaccaacact ttcagagctg
    18661 atagaagctg ctgagaaggc agtaaaacca gacgactggt accgacaaag tttaatcttg
    18721 gagaagttcc acggcatgtc aaaaactact ttagttgaat actgcaagga aatggaaaca
    18781 attcctgaat ttgcagaagg aatacttcgt ccaggtcact caaccacatt tattcattac
    18841 catactttta tttggttttt aaagtggaaa gacgcaaaca aatatcgtgt aaaaagttta
    18901 tcgccttcag atgttttgaa ggaggcaagt taatgggcaa attcaacaga gcactagtat
    18961 tcagcgcacc gctaatcatc tacgctttag gcctctgggg aagcagacaa gcgttgatag
    19021 ggacaatcgt ttacatggtt tggattttta tggggcttga tgaagctgag cacagagcga
    19081 aaaaagccag tcgggaggga ctgactaaaa tatgattttt ttatttttgt tcctttgggc
    19141 tgctattgcg tacttcatcc agctccttgt tgtcaaagtt gctcttattt cttttaggaa
    19201 gaacaaaaga aaatagcgct ataaatgaaa gataagtagc cgctacatta aactgttctt
    19261 ttgaaaaagt tacagtcatc ggaatgataa ttgatattga ggaagtaatc ttaattaaaa
    19321 atatagcaat attttcaaca tcgcgttggt ttttatttaa cattgtccaa catgtt
//
LOCUS       DACDSY010000047        19114 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDSY010000047 DACDSY010000000
VERSION     DACDSY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.047"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            418..840
                     /locus_tag="IWK44_001331"
     CDS             418..840
                     /locus_tag="IWK44_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9549867.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            837..1067
                     /locus_tag="IWK44_001332"
     CDS             837..1067
                     /locus_tag="IWK44_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9549868.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            1565..1765
                     /locus_tag="IWK44_001333"
     CDS             1565..1765
                     /locus_tag="IWK44_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9549869.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            1792..2985
                     /locus_tag="IWK44_001334"
     CDS             1792..2985
                     /locus_tag="IWK44_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9549870.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(3053..4618)
                     /gene="guaA"
                     /locus_tag="IWK44_001335"
     CDS             complement(3053..4618)
                     /gene="guaA"
                     /locus_tag="IWK44_001335"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9549871.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(4677..5600)
                     /locus_tag="IWK44_001336"
     CDS             complement(4677..5600)
                     /locus_tag="IWK44_001336"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP9549872.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            5796..6401
                     /locus_tag="IWK44_001337"
     CDS             5796..6401
                     /locus_tag="IWK44_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9549873.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            6709..7725
                     /locus_tag="IWK44_001338"
     CDS             6709..7725
                     /locus_tag="IWK44_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9549874.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            7866..9167
                     /locus_tag="IWK44_001339"
     CDS             7866..9167
                     /locus_tag="IWK44_001339"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP9549875.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            9213..9875
                     /gene="deoC"
                     /locus_tag="IWK44_001340"
     CDS             9213..9875
                     /gene="deoC"
                     /locus_tag="IWK44_001340"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP9549876.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            9896..10294
                     /locus_tag="IWK44_001341"
     CDS             9896..10294
                     /locus_tag="IWK44_001341"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP9549877.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            10360..11442
                     /locus_tag="IWK44_001342"
     CDS             10360..11442
                     /locus_tag="IWK44_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9549878.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            11654..13216
                     /locus_tag="IWK44_001343"
     CDS             11654..13216
                     /locus_tag="IWK44_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9549879.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            13209..14342
                     /locus_tag="IWK44_001344"
     CDS             13209..14342
                     /locus_tag="IWK44_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9549880.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            14342..15298
                     /locus_tag="IWK44_001345"
     CDS             14342..15298
                     /locus_tag="IWK44_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9549881.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            15397..16563
                     /gene="deoB"
                     /locus_tag="IWK44_001346"
     CDS             15397..16563
                     /gene="deoB"
                     /locus_tag="IWK44_001346"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP9549882.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            16587..17405
                     /locus_tag="IWK44_001347"
     CDS             16587..17405
                     /locus_tag="IWK44_001347"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9549883.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            17422..18129
                     /gene="deoD"
                     /locus_tag="IWK44_001348"
     CDS             17422..18129
                     /gene="deoD"
                     /locus_tag="IWK44_001348"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9549884.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(18427..19113)
                     /gene="gpmA"
                     /locus_tag="IWK44_001349"
     CDS             complement(18427..19113)
                     /gene="gpmA"
                     /locus_tag="IWK44_001349"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9549885.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
BASE COUNT         6064 a         3474 c         4158 g         5418 t
ORIGIN      
        1 tgaatccaat ttcaaataga aaggtcgctg attttattta cctatacaac tttataaaat
       61 ttagtcatac tcctagaaag tagataaaat tggcgactta atggagggaa aagtcgccat
      121 gagaaagata atctgtcatc tagttctctt aattaagttc ttatcaaaaa aacttatcca
      181 cctgtactgg atagatatga ccatcaatgt ggttttgcag attacataga tagaaaaatc
      241 aaagcatatg aacagagaaa acaatctgtt tatgtgcttt tttggtcact taataaatat
      301 cttttaggat ttctcttcat ttatgcaaca aatcccccgt tcgtgtcggg gtttagtatg
      361 aagggagata aagaagcaca tgccactccc tttcacgaca gaaagggggt gaaaagcatg
      421 aaaccatctt cttttcaaga aaccatagaa aatcagtttg actatatctg caaacaagtt
      481 attgaagatg aacgaaaaga ttacttcaaa catctagaca gactatcagc aaaagaagtc
      541 tcgttttctg atatagataa ctaccttgtc aactcttttt ctactgttga tacgtacgtc
      601 actgatttcc aactatttaa attatttgga ttaagaatca atgttgaaag tgatttatta
      661 agtgaagccc tgcgtcatct ttcagagaaa aaacggaaca ttattttact tcattacttt
      721 atggatatga gtgatgtgga gattgcggag ttactaaatt tgaatcgttc gactgtttat
      781 cgtcatcgaa taagtggact agccatgatt aaagaattca tgaaggagtg taaagaatga
      841 aaacagctta tccaagggtt cccttccccc tcattgtaaa ggcgacagat ggcgacgtgg
      901 aagcaattaa tcagattgtt aaacattatc gagggtatac ctcaaaacgt tcattacgtc
      961 gcatgacaga cgaatatgga aattctcata tggttattga tgaaacccta cgtggcagaa
     1021 tggaaacgaa actcatctct aagattttat cgttcgaaat taaatagtcc cattttttcg
     1081 tggaagcgtg tcgaatcgta tccacgcttc ccgatttggg aggtttgtta ttccatcaaa
     1141 gcatattgtg tgttcagtag gttttgatag gctaacaagc caaacgttct ttgacaacca
     1201 aataaaggca atcgaatacg tttcggtaag aaaagagctg atgaactaac acgccatgac
     1261 ctacaaaatt agtgagcgat tcatgttagt aattcgaaag taatctttag caggattgct
     1321 gccatgactt tcttatcgtg ataatgatac tcccgtatag ccatagtccg agcgttagaa
     1381 gcgtcacagg caatgggtac ggctacatga gaaccatgca gaagtgaaat tcctgtgagc
     1441 tttgctagag ctgttcgatt gctaagatat tcttctcttg tcagtgtatt attataaaac
     1501 tatttgatat aattataggt gaatattgga gttttcctag cctataattg ggaaaggagt
     1561 aataatgata acggtcacaa aaaaggattt agttgagcta ggatatggaa cttctttttc
     1621 agcagacatc ataaaaaaag caaaagaatt gatgattaca aaagggcata tctactatca
     1681 atctcgaaag ctagatagag tccctcgtga agctgttgaa gaaattctag gaattcggtt
     1741 ttcaaatgaa aacaggtctg agtagttttt gcacagtaag gagtaaaaac tatggttaaa
     1801 gacccaataa aaaaagcaaa gaatggaacc tattattttc gtgcaaatct aggttatgat
     1861 tctaaaggga aaaaaattca aaaatatcgt agtggattca ctactaaaaa agaagcaaga
     1921 gaagaatatt cgaaactgct attgatgaaa ccagaagaat taagtgagaa taaggataaa
     1981 atgacgttta aacattatat ctttgaaatt ttccttccat ggtataagac acaggtaaag
     2041 ctcagaacat atgaaaatcg cttaccaact attaagaagc atttctctta ttttgataaa
     2101 atggctgttt ccgatattga gcctattgat gtgcaaaatt ggcaactaaa attatcaaag
     2161 aagtgtaagt cttcttatgt aagagctgtt caaggactat tttcagttgc tatggatcga
     2221 gcaattgtcc ttgggataac caccacaaat ccatctaaga ttattggcaa tgtgaaaaag
     2281 caaaagtcta aaattgaatt ttggacaaaa gaagaatttg aaaaagtaat ctctctaatt
     2341 tacaaagaag attactatca acacttttta tttatctctc tgtggttttt atttatgact
     2401 ggaatgcgaa ttggtgaagc tactgccatt caatgggaag atattgattt tgactcagga
     2461 gtactaacca tcaacaaaac actctattac aagaatcaat cgaattatag atttgttgaa
     2521 ccaaaaacaa aggcgagcgt tcgtcatatt gtattagata aatgcacatt aacttattta
     2581 tccgaatgga aggaagtaca acaaaatcta attcagacag actttgtaat gagctataac
     2641 ggtattccta cccaaaaaca tacactatca tatgctattg aacgctatgc taagctggct
     2701 ggtatccatc gaatcagaat ccatgggtta cggcattctc atgcttcctt gttaatcagt
     2761 atgggagaaa atccacttat cattaaagat cgtctaggac acgaagatat tgagacgact
     2821 ttaggaacgt atggacatct ttatcctaac agtaatttcg aggtagctaa caaattaaat
     2881 ggagccattt cctttaaaga agcaaaacaa aattatgata cttcaccaaa gaatcaattt
     2941 actgtagact acttgcgaaa caaagaaatg aaaaaagtgc aataaaagtg cagtaaaaag
     3001 ataagaagag ccagaaaccc ttggtataag aggatttcta gctctcctac aactattccc
     3061 actccactgt tgctggtggt ttagaagtga tgtcatacac gatacggttg acgtgggcta
     3121 cttcgtttac gatacgtaca gagatttttt gcaagacttc ccatgggata cgggcaaagt
     3181 cagcggtcat tccgtcgatt gaagttacag cacggatacc gactgtgtag tcgtaagtac
     3241 gtccgtcacc cataacaccg acagagcgga tccctggaag aacagtgaaa tattgccaga
     3301 tatcacggtc aagtccagca gctgcgattt cttcacgcaa gatagcatca gattcgcgta
     3361 cgatttctag cttgtcttca gtgatttcgc caagaacacg aataccaaga ccaggaccag
     3421 ggaatggctg acgccaaacg attgaatcag gcatgcctaa ctgagtccct aattcacgga
     3481 cttcgtcttt gaataatgtg tttaatggtt cgatcaattc aaattgcata tcttctggca
     3541 gtccacctac attatggtga gatttgatcg tctgtgctgt ttctgtacca gattcgataa
     3601 cgtcagtata caacgttcct tgtgctagga aagagacgcc ttcttcacca gcaagttttg
     3661 ttgcttcgtc atcaaaaaca tatacgaatt cattcccaat gattttacgt ttttgttctg
     3721 ggtcagatac acctttcaac ttgtctaaga agcgatctct tgcatcgact ttgatgatgt
     3781 tcaagccgaa ttttccgcct agactttcca ttacttgttc cgcttcgcct ttgcgtagca
     3841 gaccgtggtc aacaaagata gatgtcaatt ggtcaccgat cgctttttgt aaaagaaccc
     3901 caacgacact agaatcaacg ccacctgaaa gaccaagcaa gacttttttg tcaccaactt
     3961 gttcacggat tttttctact tgcatatcga taaagttatc cattgtccag ttaccttcac
     4021 agtgacaaac atcaaaggca aagtggcgca gcaattcgat tccgtattcg gaatgacgta
     4081 cttctgcatg gaattggata ccataaaaat tacgtgttgt gtcttggata gaagcaatcg
     4141 gacaatcttt gcttgttccg actgtttcaa agccttcagg aactttagtt accaagtcac
     4201 catggctcat ccatactgtt tgttgttttg gtgtttcttt aaacaagaca gcttgatcgc
     4261 ttgttacttc taacaaggct ttcccatatt cgcggttttt agcaggctct acggaaccgc
     4321 caagtttgta cgtgatcaat tgcataccat agcaaatccc taatacagga atacctaaat
     4381 tgaagatctc aggatcgatc ccaaatgcat tctcatcata tacactgttt ggtccgccag
     4441 aaagaatgat tccctttgga ttcatcgctt ttacttcttc tgcagtaatg cggtggctca
     4501 ataattcgga aaagactcca agatcacgaa tacgtcttgt gatcaattgg ttatactgac
     4561 taccgtagtc taatacgata atcttttcaa cgttcgtcat atcggcaacg tttgtcaaat
     4621 ttattacccc tatcccttta aattttatct caaaatagtc aaactatttt aaaatgctaa
     4681 tattttcgca tgaaaatcga atcgatgata tgattttcag ttttatggag aattatatcg
     4741 gctcttcctc gtgtaggcaa gatatattcc tgtaaatttt tcaaattcac atttttccat
     4801 acatcttttg ccattttaaa ggcttcttca cgatctccga tagcaaaacg ataataataa
     4861 ttctctggat tttggaaagc cgtatccagt aacgcaccaa aacgatccaa ataccatttt
     4921 tcgatcagca cgggatcggc atcgacatag acagaaaaat caaaaaagtc gctgacgtag
     4981 atttgctgat tcgcaggtaa ttgaagcgta ttgatcccct caacgatcaa gatatcaggc
     5041 tgctggatca attcgtactt gtctggaatg atatcataca catcatgtga gtaaacaggt
     5101 acttttatct cttcctgacc agattttaca cggttcaaga aatcgatcag catctccata
     5161 tcataacttt caggaaatcc tttacggtcc atgattccac gctcttttaa tatacgattt
     5221 ggataaagaa atccgtcagt cgtgatcagc tgaacgtttc ttcgtttgaa ggtacgagac
     5281 aaaatcgtct gcagcagtct ggctgttgta ctttttccaa cggctacact tccggcgatc
     5341 ccgataataa aaggcggtac ttctacgtat ttatgaagaa ataaaccttt actcaagaac
     5401 aaagattcaa attctttcat ataaagataa atcagatgag caagaggtac atagatatct
     5461 cgaacatcct gcatggaaat gcggtcattc aaacttttga tattatctag ttcatcttgt
     5521 gtcaaaggtg cttgcccatt acgataaaat tctcgccatt cttctcttgc cacacggtag
     5581 tagttcattg gatcattcat attagcctcc tgcgttctcc catcaatgga actattttaa
     5641 cacaaaataa acgcaaaccc caccacttaa gagagattgc cgatcttttt gtataatctt
     5701 tataatagaa agaaacggag tcttattctt gacttgcgca cggatacata aaaggataga
     5761 ctgttgtcat gaaagaaatg agaaggatgg aagacatgac aaatcattac tattctgaac
     5821 gcccagatac ggcgcacgat tttgaacaat gggcttttga gttaaaaggc agaacctttc
     5881 aatttgtaac agattctggt gtattttcac gtgaaacagt ggattatggt tcacgtgtcc
     5941 tgattgatgc tttcgattgg gaagagttac ctgatgaagg aaagatctta gatgtgggat
     6001 gcgggtatgg accgattggg ttagctttgg catttgcgac acagcggttt gttgaaatgg
     6061 tcgacattaa tgaacgagcg gtaagtctag cgcaaggaaa cgcaaagcgt aaccaaatcg
     6121 aacatgttga tatccatcaa tcgaatatct atgaagcagt tcatgaagaa acctatgcag
     6181 ctatcgtaag caatccgccg atccgtgctg gaaaaaaagt tgtacatgaa attttgacag
     6241 gcgcatatcc tcggttgaag aagggcggta cactgacaat tgtgatccag aagaaacaag
     6301 gagcgccgag tgcgcaaaag aaaatggaag aaacatttgg taatgcagaa atcgtcacaa
     6361 aagataaagg atattatatc atcaaaagtg tgaaagaata actagctgaa aaatagtgga
     6421 gcgacgaaag agtggaactg ggcgtgcatg tacgggtacg tatcagttct ttttttgttg
     6481 agaagttttt aaaaacaaag aactattttt tcttcaaaaa gaaatagtag gaacagagtc
     6541 gttgtttttt acgataaaat aaataagata cagaacttgt atagaaggct gccaagtcgg
     6601 tagataaagg aggtttttat caaaaaacat actttccttt tttctctatt cttttcttgg
     6661 gggttttggt acactgatag ggaatttaga tagaagggag ccaataggat gaagctgacc
     6721 atcagagata tcgcagaaat ggcaggcgta tcagtaacaa ctgtctcaca gattttgaat
     6781 aacaaaggaa gcagatttag tgataagaca agaaaaaaag tgttgtcgat cgtgaatgaa
     6841 catcattata agcccgatta tttcgcttca aatgtgatca atcgacattc aaagaccatc
     6901 ggaatgatcg tccctgatgt cactgatttc ttcttctcca aactgatcga aggtgtagag
     6961 agctatttga atcctttggg ctatgtgatc atgttatgca attctcgtca tagccaagaa
     7021 aatgagatca agtatttaca agagctttcc caccgctcag tggacggaat cttgctggcc
     7081 acaccaaata ttttacctga gaaatatgca ttagacagcg gtttttatca aaaaatgcca
     7141 atcattttaa tcgatcgcgg attgaatcgt cgggataacg gacggcttat cgtaaaggaa
     7201 tatgagggcg cctatcaagc tgtttcgctg tttattgaaa atggccatac gaagatcggc
     7261 atgctgaagg aaaccactgg atactatcaa ttagaagaac gtttcaacgg atatcgtcat
     7321 gcgctgaaag atcatggcct gccttttaat gggaaatttg ttgaacatgg agagttgact
     7381 gtccaaggtg gatatactgc atcaaaaaaa ttactcaaac ataaagatat cacggctatt
     7441 ttttgcggga atgatgccat ggcgatcgga tgctatcagg cgatcgatga attgggcaaa
     7501 aaaatcccag aagatatctc agtcatagga ttcgatggat taaagctgtc agaatacatg
     7561 attccgcaat tgacgacagt gcagcagcca agttttgata ttggatttta tgctgcacgt
     7621 ttcctgatcg atacgatcga atttcctcaa agaaaagttc cgaacaaagt gttcgaaaca
     7681 aaattaatta ttcgtgagag cgtaaaagta ttgacaaagg gctaactcgg cggtatattg
     7741 cttatgtaag catttacaaa ggctagttag tgcttttttt atttttagta aaccagttta
     7801 ctaaagtggt ttacctttcc taaaaataat gtataatacc gaatgaagac aaggaggatc
     7861 aggcaatgcg aatggtggat ctgattgaaa aaaagaggga tggaaacgag ctgtcgaaag
     7921 aggaaatcga gtacatcgtc acgaactata cgaatggaaa gattccagat tatcaagtca
     7981 gtgcattatt gatggcaatt ttttatcaag acatgacaaa tgaagaaatc acgaacttaa
     8041 cattggcgat tgctaattct ggtgatgtca tcgatttgag ttctcttgaa ggaataaagg
     8101 tagataagca ttcgacaggt ggcgttggtg acacgaccac attgatttta gcccccctag
     8161 tagctagtgt cggagtgaca gtggctaaaa tgtctggacg cggtctaggt tacacaggtg
     8221 gtacactaga caagctagaa gcaattcccg gatttcagat cgagctttcg gatgaagaat
     8281 ttgtacgcat tgttaatgaa agtaaagtag cggtaattgg tcaatcagga aacttggcac
     8341 cggcggacaa aaaattgtat gccttgcgtg acgtcacagc gaccgttgat tctcttccgc
     8401 tgattgccag ttcgattatg agtaaaaaaa tcgcggcagg agcagatgca atcgttttgg
     8461 atgtgaccac tggagacggt gctttcatga agaacatcga agatgcaaga cgtctggcaa
     8521 agaccatgac gagcattggg aaactggcta accgagaaac agtggcggtt atctcagata
     8581 tgtcagaacc tcttggcgaa gctatcggaa atagtctaga agttgtcgag gcaatcgaaa
     8641 cattacaagg aaatggtcca gaagatctag tagaaatgtg ctacgcctta ggaagccaaa
     8701 tggttgttct tgctggcaaa gctaagacaa ccgatgaagc aagaacgttg cttcaagaag
     8761 ctctggaatc aggtaaagcg ttagcaaaat tcaaagaaat gatccaaaat caagggggag
     8821 acccagcgat cgtggaacat ccagaacgaa ttttgactgc acgctacaca atggagcttc
     8881 ctgctaaaca aagcggagtt gtgtcaaaaa tcgtggcaaa tgaattagga attgctgcaa
     8941 tgatgctagg tgctgggcgt aaaaccaaag aagaagatat tgatcacgct gttggattga
     9001 aactgcataa aaaaattggt gacactgtga caaaaggtga gtcactattg acgatctata
     9061 gtaacgacga agaaatcact tctgtcattg aattattata taaaaatatt gaaattggtg
     9121 aatcggcaat gaagccaacg ttgattcatg acatcatcac agaataaagg aaaaaagcgg
     9181 actttaccgt tttaaagaaa aggggaaaag aaatggaact aaaccgaatg atcgaccata
     9241 ctattttgaa agcagacgca tctaaagaag ctgtgatgca aattatcgaa gaagcaaaaa
     9301 aataccattt ttattctgtc tgcatcaatc cagcatgggt tgcgctagcg aaagaacaat
     9361 tacaaggtga gccagtagcc gtatgtacag taatcgggtt tcctctagga gcaaacacac
     9421 ccgagacaaa agcatttgaa acaacagatg ccatcaataa cggtgcagat gaagtcgata
     9481 tggtcatcaa tatcggtgct ttgaaatcaa aggatgatca acaagtccaa aaagatatcg
     9541 aagcagttgt tgaagcagca aaagatcgtg cgcttgtcaa agttattatt gaaacatcgt
     9601 tattagatag agaagaaatc atccgagctt gcgaaatagc aaaagctgca ggcgctgatt
     9661 ttgtcaaaac atcaactggt ttctcaacag gcggagcaaa ggtagaggat gtccgtttga
     9721 tgcgtgaaac tgtaggacca gaaatgggtg tcaaagcttc cggcggtatc cataacgaag
     9781 aagaagcaat ggcaatgatc gaagctggtg ctacaagaat cggaacaagc gcaggagtag
     9841 caatcgtatc tggttcaact ggtgaaggct attaattttt tcaaaggaga agctcatggc
     9901 agaactcaaa caagaatgga tcaacacagc tattgaagcg ttagataaag cctatgttcc
     9961 ttattcccat tttccggtag gcgcttgtct cgtaacggaa tcaggcaaga tttatcaagg
    10021 catcaacatt gagaatgcat catttggatt aacgaattgt gccgagcgca cagctttttt
    10081 caaagcagta tctgaaggag aaaaaacatt tacgcatcta gtcgtagcgg ggcacacccc
    10141 cgatcctatc tctccttgtg gtgcatgtcg acaagtaatg gcagaattct gttcccctga
    10201 tatgccggtt acgctagtag gagacaatgg tgtgacgaag gcaacgaccg ttcgtgcact
    10261 gctgccttat gcatttactg aaaaagatct ataactcatg ttcattcagt cagctgactg
    10321 ttgaaaaata aaacaattat tttaggaggc tttacagaaa tgaaaaaagc aaaattattt
    10381 ggtttaggtg ctgtcgcact agcagcaggg ttatttttag gtgcatgcgg caacaacggc
    10441 tctactgatt catccggtgg caaaagctca tcagacacaa caactgcagc attgatcaca
    10501 gacacaggtg gtgtagacga ccgttcattc aaccagtcag cttgggaagg actagaaaaa
    10561 tgggggaaag atcaaggact ttcacgagga aatgatggct tccaatattt ccaatcttca
    10621 aatgaatcag attacatccc aaatattgac caagcattga atgctggatt caaaacgatt
    10681 tttggtattg gttataaact aaaaccagcg atccaagaac aagcaacaaa caatacaggt
    10741 acgaactttg ttattatcga tgatgtcatc gatggattag ataacgttgt atctgcaact
    10801 ttcaaagaca acgaagcttc ttatcttgca ggtgttgcag cagcttatac gactgaaaca
    10861 aacgtagtcg gctttatcgg cggtgtaaaa ggtgaagtaa tcgatcgttt tgatgctgga
    10921 ttcaaagccg gtgttgatgc aggtgcgaaa gaattaggaa aagaaatcaa agtattgaat
    10981 caatatgctg gtgacttctc tgctccagat aaaggacgtt caattgctca aggtatgtat
    11041 gcacaaaacg cagatatcat cttccatgct tcaggcggta caggtaacgg tgtattccaa
    11101 gaagcaaaat cattgaacga atcaggcgac aaaaaagttt gggttatcgg tgttgaccgc
    11161 gaccaatcag acgaaggcga atacacatta aacggcgaaa agaaaaactt cacattgaca
    11221 tctacactga aagcagttgg aacagtagta gaagatttgg cacaaaaatc agcggacggt
    11281 aaattccctg gcggcgaaca cactgtttat ggattgaaag aagacggtgt tggtctaaca
    11341 gaaggacaac tttctgacga agcgaaaaaa gcagttgatg aagcaaaaga aaaaatcatt
    11401 tcaggtgacg taaaagttcc agaaactcct gaagaaaact aaaaccattt ctatagcctt
    11461 gttagacaca tgtttgacaa ggctatggtt tgattagaac aagaattgtt catttatcca
    11521 agctgccttg tgacagtttg gataagggaa caactccctg aagtaatgtt tgtttcaaaa
    11581 gaagaacgct cactaagcgt gaatcttagt caatcgttgc cctagataga aatcgttagg
    11641 atgtgaaaaa aaggtggttc aagaaaatat tgtcatagaa atgcagaata ttaccaaaga
    11701 atttggaaca ttcaaggcaa atgacaacat caatttacaa gtaaaggcag gagaaattca
    11761 tgcgttgctt ggagaaaacg gtgctggcaa atctacattg atgaacgtgc tttccggatt
    11821 attagagccg acatcaggga aaattttgat gcgtgggaaa gaagtaaaga tcacaagtcc
    11881 gaccaaagcc aaccaattag ggattgggat ggtccatcag cactttatgc ttgttgatgc
    11941 ctttactgta acagaaaaca tcgtgttggg aagcgagcct agtcgcgcag ggatgcttga
    12001 ccataaaaaa gcacgaaaag agatccaaaa agtttctgaa cagtatggat tgtcagtcaa
    12061 cccagatgct tacgttcgtg atatttcagt tggaatggaa caacgggtag aaattttaaa
    12121 aacactttac cgaggagcag atgtactgat ttttgatgag ccgacagctg tattgacccc
    12181 tcaagaaatt gatgaattaa tcgtgatcat gaaggaatta gtcaaagaag gcaagtcgat
    12241 cattttgatt acgcataagt tagatgaaat caaagcagta gctgaccgct gtacagttat
    12301 ccgccgtgga aaaggaatcg gtacagtcaa cgttaaagac gtaacctcac aacaattagc
    12361 cgatatgatg gttggaagaa cggtttcatt caaaacgatg aaaaaagaag cgaagcctca
    12421 agaagtcgtt ttgtctattg aaaatctagt ggtgaaagaa aatcgtggat tagaagccgt
    12481 gaaaaacttg aacttagagg ttcgtgctgg cgaagtactt ggtatcgctg gaatcgatgg
    12541 aaacgggcag tcggagttga tccaagcttt gactggtttg cgaaaggcag aaagcggaca
    12601 tatcaagcta aaaggggaag acatcactaa taaaaaaccc cgaaaaatca ctgaacatgg
    12661 tgtaggacac gtgccagaag accgtcataa atacgggtta gtcctagata tgacattatc
    12721 tgaaaatatc gcactacaaa cctatcatca aaaaccttac agtaaaaacg gcatgctgaa
    12781 ttattcagta atgaatgaac atgccagaga attgatcgaa gaatatgatg ttcgaacaac
    12841 gaatgaactt gttcccgcaa aagctttatc aggcggaaat cagcaaaaag caattatcgc
    12901 tcgtgaagtc gaccgagatc ctgatctgtt gattgttgca aatccaactc gtggactgga
    12961 tgtaggtgcg atcgaattta ttcataaacg tctgatcgaa caaagggaca aatacaaagc
    13021 agtgttactg atcagtttcg aattagaaga aattttaaat gtttcggatc gtattgctgt
    13081 tatccatgaa ggagaaattg tcggtatcgt tgatccgaaa gaaacatctg aaaatgaatt
    13141 aggtctattg atggcgggct ataccttaga agaagcacga aaagagttag aaaagggggc
    13201 tggcgcacat gaatgatcgc agcgaaaaat tccgtaattt attagtgcct gtactttcag
    13261 ttgttttagg attgatctta ggcgcgatat tgatggctgc ttttggtttt cagcccatcc
    13321 aaggttattc ttctatgcta gatgcagccc ttggttcgca aagagggata ggtgaaacgt
    13381 taaggcaagc aacgccgttg atttttacag cattaggttt ttctgtagct aactcagctg
    13441 ggtttttcaa tattgggctt tctggacaag cactttgcgg gtggatcgtc agtgtctggg
    13501 tggcacttgc gtttccagat ctgccaaaag ttgttttgct tccgttatgc gtgatcttag
    13561 gtgccttagc tggtgcgatg gcagcggctg ttccaggttt acttcgagca ttttttggaa
    13621 caagtgaagt gatcgtaaca atcatgatga actatatctt attatacgta agcacatttg
    13681 ctttacatga agtgatgcct gaaagctatc gctcaagttt ggactcttcc aatatgatca
    13741 gtcaaaatgc ctcactgcgg ttaccatggt taacacaagc ttttggcggc tctcgcgtaa
    13801 actcaggatt atttttagca atcatcgcgc ttattttcgt ttggctgctg atgaagaaaa
    13861 cgactttagg atttgaaatc cgttctgttg gattgaatcc ttttgcttct gagtatgcag
    13921 gaatgagtag taaacgtacg atcgttttgt cgatggtcat ttctggcgct ttggcagggc
    13981 ttggcggtgt ggttgaagga ttaggaacgt atcaaaactt ctttgttcaa acgacttcgc
    14041 tatctattgg atttgacgga atggctgttt ccttgcttgg ttctggatca gcaatcggta
    14101 ttcttttatc agcgatcttg ttcagtattt tgaagatcgg tggacttggc atgcagacag
    14161 gagcaggtgt tccgtttgag atcgtcaatg tatcgattgc tttgattatc ttctttgtcg
    14221 ggatcaactt cctgattcgt ttcgtcatgg ctaaagtctt ccaagggaaa aaacaagaag
    14281 agatcgttgc gacaatagaa tcgaaaccaa cgaatcaaaa ccatgaaggg ggcgaactat
    14341 aatgtcgact atcgaattaa ttgccttgat ccttacttct acactggttt attcaacacc
    14401 gctaattcta acgtcactag gcggaacttt ttctgaacga agcgggatcg taaacgtagg
    14461 gctagaggga atcatggtca tgggtgcttt tagttccatc gttttcaact tgacgatgag
    14521 tccatcactt ggttctatga caccatggat cgctatttta gtgggtggcg tcgtaggtat
    14581 cctattttct ctgattcacg cagtagcaac agtcagtttg cgtgctgacc atattatcag
    14641 cgggacagta attaatctga tggcacctgc tttaggtgtt ttcttgatca aagcatggta
    14701 tggtaaaggt caaactgata acatcacgca gaatctgggg tatttttctt tcccaggatt
    14761 agcagatatt ccggtcattg gaccaatttt ctttaaaaat acattcttac ctgcttattg
    14821 tgctattttg attgctgtcc tagcttgggt gatcatcttc aagacgaaat tcggcttgcg
    14881 cctccgttca gttggtgaaa atcctcaagc tgccgacaca ttaggtatca atgtatatgc
    14941 tatgcgttat tcaggcgtac tgatttctgg cttgttaggc ggtatgggcg gtgctgtttt
    15001 tgctcagtcg atttccggta acttttcagc tggtacgatc gttggacaag ggttcatctc
    15061 catggctgct atgatctttg gtaagtggaa tcctttaggg gcaatgggtg cctcattgtt
    15121 ctttggtttt gctcaaagct tgagtatcat tggcgcacaa ttaccagtta tttcttcgat
    15181 tccgccagta ttgctgcaaa ttgctccata tttgctgaca atcattgtgc tggttgtttt
    15241 cttagggaaa gcttctggtc caaaagcaaa cgggaagaac tatatcaaat caaaataatc
    15301 ttttagcgaa taaaaagggt gggttacggg aagtgttttt cgtctcgccc tttttgtttt
    15361 aaaggcaata aaaataaaaa gaaagaagga ataatcatgt ttaaacgcgt acatctgatc
    15421 gtcatggatt cagtaggaat tggagaagca ccagatgccg ataagttcgg tgatgttggt
    15481 tctgatacat taggacacat tgcgaaagaa gcaggattga acgttccaca tttagagcag
    15541 cttggtttag gaacgatccg tcctcttgaa ggtgtggaat cagtgaaaga tcatcaaggc
    15601 tatgcgacga aattagaaga agtttctgta gggaaagata cgatgacagg ccactgggaa
    15661 atcatgggat tgaatatcaa aactccattt cgtgtgtttc ctaatggatt ccctgatgat
    15721 ctgatcaaac aaatcgaaga attttctggc cgtaaagtcg tatgtaacaa accttatagc
    15781 ggaacagctg ttattgacga ttacggtgaa caccaaatga aaacaggcga tttaattgta
    15841 tatacttctg ccgatcctgt ccttcagatc gcggcacatg aagaaatcat tcctttagaa
    15901 gaattatatc ggatttgcca attcgttcgt gatataacaa aagatgatcc gtatatgatt
    15961 ggccgaatca ttgcacgtcc gtacgtgggt gaaccaggag acttcacacg aacaagcaat
    16021 cgccatgatt atgcgttgaa tccattcgga cacacagtcc ttgattcttt gaaagaagca
    16081 ggaaaggacg tgattgctgt tggaaagatc aacgatattt tcaacggaca aggaatcact
    16141 gaatctgtcc gcacgaaaag caatatggat ggcgttgacc agctgctgaa agttatgaaa
    16201 aacgattttt caggaatcag ctttaccaat ttagtagatt ttgatgcatt atacggtcat
    16261 cgtcgggatg tagtcggcta tgcgcatgcg attgaagaat ttgacttacg aattccagaa
    16321 ttgcttgaag cgatggaaga agacgacttg ctaatgatta cagccgatca cggaaacgat
    16381 ccgacgttta ctggtaccga tcatacgcga gaatatgtac cgttattggt ttacagtaaa
    16441 aaaatgaaag aacaaggggc attgcctcaa ggatattatt cagatatttc agcaacgatt
    16501 gctgagaact ttagtgtttc ttcgactgaa aatggcgaaa gcttcttgaa attattaaaa
    16561 taatcttttt aaataggaga aagcaaatga ccaagttaag cgagatgtta caagaaacga
    16621 cccagtttat ccttgataaa ggagtaaaag aaattgattt tggtttgatt ctaggttcag
    16681 gattaggtga attagcagat gaaatcgaag atgctgtaat gatttcttat gatgagatcc
    16741 cgttctttcc tgtttcgaca gtagttgggc atgcaggaaa gctagtttat gggtctctgg
    16801 caggcaagaa agtgttagcc atgcaaggga gattccacta ctatgaaggt cattccatgc
    16861 aaacagtgac ctatccagta cgtgtgatgg ccggactagg ggcccattca gtcattgtga
    16921 ctaatgcatg tggtggtgtc aacgaatcat ttgtaccagg agatttgatg ctgattaccg
    16981 atcatatcaa ttttactggt gaaaatcctt tgatcggtcc gaatgaagat gcaatgggcc
    17041 ctagatttcc agatatgagt caagcttata cgcctgacta tcaagcaaca gcaaaagcag
    17101 cagcagaaaa actaggtttg gatcttaaag aaggcgtata tatgggctat tctggaccaa
    17161 cttatgaaac gcctgcggag atccgaatgt ctcggacaat aggagccgat gcagtaggga
    17221 tgtcgactgt tccagaagtc atcgtagcag ctcacagcgg attaaaagtg ttaggaatct
    17281 cttgtattac caacttagca gcaggtatgc aagcgaatct gagccatgaa gaagttgtag
    17341 aaacaactca gagagtcaaa caatctttta aagcattggt taaagaaaca ttgacattat
    17401 tgtaatagga ggaacaataa aatgagtgtg catattgaag cgaaagaagg cgagatcgcc
    17461 gataagattt tacttccggg agatcctttg cgagctaaat atattgcgga gacttttttg
    17521 gaagatccag tttgttacaa tcaggtacgc ggtatgttag gctttaccgg caaatataaa
    17581 ggagaacgta tttctgttca aggaacagga atgggaatgc catcagccac catctatgcc
    17641 catgaattga tccagtcgta tggtgtaaag aaattgattc gagtaggtac atgcggtgca
    17701 ttgtcaaaag acgtccatgt tcgtgattta gtactagcac aaggtgcagc gaccagttct
    17761 tcgatgatcg aaaaaaattt ccaagccttt cattttcctc caatcagtga tttcaatcta
    17821 ttactgaaag catatgagat agctaaagaa aagggctata cggttcatgt aggaaatgtt
    17881 ctttcggaag attctttcta caaagatgat ttgacagaga cgttccaatt agctgaactt
    17941 ggcgttttag gagtggaaat ggaagcagcg gctctttatt acttaggcgc gaaataccat
    18001 gtgcagacat taagtttgat gaccgttagc gaccatttga tcaccggtga agaaacaaca
    18061 gctgcagaac gtcaatctac gttcaatgaa atgatcgaag taggattgga aacagcgata
    18121 gcagaataag aaggaaatga aaaagctgcc ctgcattaag taataagaac atccaagcaa
    18181 aaatagctcc tcatgttttt gacttggctg gacttattat tgcaaatgta agcttttgaa
    18241 caccgtttac caagaggttg tgaatctgcc gtttaactct gagtcacaag gaacaatttc
    18301 aaaaaacagc ttttcatgtt tgatgaatat tgtgacttgt gaccgagaag ttggcaggag
    18361 aaaaccgttt atacgaaaaa agggactgtg acaaatcgtg tcacagtccc ttttttatag
    18421 tgttttttat aggtagtatt ttttagcaac tgttaagtca tcatttaatt cataaacaag
    18481 tggttgacct gttggaattt caagatccat gatgtcttcg tcagagatac cttcgatatg
    18541 tttagctagg gcgcgtaatg agttaccatg tgcagctact aaaacagttt tgttgtctag
    18601 taatgctgga gcaatttcat cttgccagaa tggcaaagcg cgttctaatg tcactttcaa
    18661 gttttctcca ccaggaatat cgcgtttgtc taacattgcg taacgacggt catttgctgc
    18721 agaaccttcg tcagtttctt ccattaatgg aggaagagta tcgtaagaac gacgccagat
    18781 gtgcacttgt tcgtcgccgt atttttcagc tgtttctttt ttgttcaaac cttgtaattt
    18841 accatagtga cgttcgttta agcgccatga tttgatttgt ggtacccaaa gttgatctga
    18901 atattctaag atcaagttac atgttttgat tgcacgagtt aaaacagaag tgtaagcaac
    18961 atcaaattcg attccagctt ctttgatttt gcgtccgcct tcttttgctt cttcgattcc
    19021 ttctggtgct agatttacat cagcccaacc agtaaattgg ttcaatgcat tccattcact
    19081 aagaccgtga cgagaaaaaa ctaattttgg catg
//
LOCUS       DACDSY010000048        18739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDSY010000048 DACDSY010000000
VERSION     DACDSY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.048"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            256..531
                     /locus_tag="IWK44_001350"
     CDS             256..531
                     /locus_tag="IWK44_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549886.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="IWK44_001351"
     CDS             544..2616
                     /locus_tag="IWK44_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9549887.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="IWK44_001352"
     CDS             2781..4667
                     /locus_tag="IWK44_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9549888.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="IWK44_001353"
     CDS             4679..5626
                     /locus_tag="IWK44_001353"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9549889.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="IWK44_001354"
     CDS             5646..6173
                     /locus_tag="IWK44_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9549890.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="IWK44_001355"
     CDS             complement(6236..6889)
                     /locus_tag="IWK44_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9549891.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="IWK44_001356"
     CDS             7021..7863
                     /locus_tag="IWK44_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9549892.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="IWK44_001357"
     CDS             8021..8917
                     /locus_tag="IWK44_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9549893.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="IWK44_001358"
     CDS             8920..9633
                     /locus_tag="IWK44_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9549894.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDALNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="IWK44_001359"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="IWK44_001359"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9549895.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="IWK44_001360"
     CDS             10168..10395
                     /locus_tag="IWK44_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP9549896.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="IWK44_001361"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="IWK44_001361"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9549897.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="IWK44_001362"
     CDS             complement(11181..11723)
                     /locus_tag="IWK44_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9549898.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="IWK44_001363"
     CDS             complement(11960..12358)
                     /locus_tag="IWK44_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9549899.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="IWK44_001364"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="IWK44_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP9549900.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="IWK44_001365"
     CDS             13471..14238
                     /locus_tag="IWK44_001365"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP9549901.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="IWK44_001366"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="IWK44_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP9549902.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="IWK44_001367"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="IWK44_001367"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP9549903.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="IWK44_001368"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="IWK44_001368"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP9549904.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3230 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt taaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctctttt
//
LOCUS       DACDSY010000049        18604 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDSY010000049 DACDSY010000000
VERSION     DACDSY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.049"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            299..1960
                     /locus_tag="IWK44_001369"
     CDS             299..1960
                     /locus_tag="IWK44_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9549905.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="IWK44_001370"
     CDS             complement(2030..2377)
                     /locus_tag="IWK44_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP9549906.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="IWK44_001371"
     CDS             2461..3402
                     /locus_tag="IWK44_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9549907.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="IWK44_001372"
     CDS             3403..4446
                     /locus_tag="IWK44_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9549908.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="IWK44_001373"
     CDS             4452..5522
                     /locus_tag="IWK44_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9549909.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="IWK44_001374"
     CDS             5512..6465
                     /locus_tag="IWK44_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9549910.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="IWK44_001375"
     CDS             6477..7487
                     /locus_tag="IWK44_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9549911.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="IWK44_001376"
     CDS             complement(7772..8593)
                     /locus_tag="IWK44_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9549912.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="IWK44_001377"
     CDS             complement(8690..8968)
                     /locus_tag="IWK44_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549913.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="IWK44_001378"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="IWK44_001378"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP9549914.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="IWK44_001379"
     CDS             complement(11724..12311)
                     /locus_tag="IWK44_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549915.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="IWK44_001380"
     CDS             12486..13808
                     /locus_tag="IWK44_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9549916.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="IWK44_001381"
     CDS             13963..14739
                     /locus_tag="IWK44_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9549917.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="IWK44_001382"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="IWK44_001382"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP9549918.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="IWK44_001383"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="IWK44_001383"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP9549919.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="IWK44_001384"
     CDS             16629..17258
                     /locus_tag="IWK44_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549920.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IWK44_001385"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IWK44_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP9549921.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT         6078 a         3345 c         3784 g         5397 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt gttaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatg
//
LOCUS       DACDSY010000050        18295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDSY010000050 DACDSY010000000
VERSION     DACDSY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.050"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(110..565)
                     /locus_tag="IWK44_001386"
     CDS             complement(110..565)
                     /locus_tag="IWK44_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549922.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="IWK44_001387"
     CDS             complement(719..1927)
                     /locus_tag="IWK44_001387"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP9549923.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="IWK44_001388"
     CDS             complement(2039..2371)
                     /locus_tag="IWK44_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9549924.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="IWK44_001389"
     CDS             2519..3394
                     /locus_tag="IWK44_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9549925.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="IWK44_001390"
     CDS             complement(3476..4057)
                     /locus_tag="IWK44_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP9549926.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="IWK44_001391"
     CDS             complement(4063..5322)
                     /locus_tag="IWK44_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9549927.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="IWK44_001392"
     CDS             complement(5324..6370)
                     /locus_tag="IWK44_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549928.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6605..6880)
                     /locus_tag="IWK44_001393"
     CDS             complement(6605..6880)
                     /locus_tag="IWK44_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP9549929.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7123..8433)
                     /locus_tag="IWK44_001394"
     CDS             complement(7123..8433)
                     /locus_tag="IWK44_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP9549930.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="IWK44_001395"
     CDS             complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="IWK44_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP9549931.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9979..10659)
                     /locus_tag="IWK44_001396"
     CDS             complement(9979..10659)
                     /locus_tag="IWK44_001396"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP9549932.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10708..11343)
                     /locus_tag="IWK44_001397"
     CDS             complement(10708..11343)
                     /locus_tag="IWK44_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9549933.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11409..12812)
                     /locus_tag="IWK44_001398"
     CDS             complement(11409..12812)
                     /locus_tag="IWK44_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP9549934.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12799..13830)
                     /locus_tag="IWK44_001399"
     CDS             complement(12799..13830)
                     /locus_tag="IWK44_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549935.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13861..14082
                     /locus_tag="IWK44_001400"
     CDS             13861..14082
                     /locus_tag="IWK44_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP9549936.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14247..14984)
                     /locus_tag="IWK44_001401"
     CDS             complement(14247..14984)
                     /locus_tag="IWK44_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP9549937.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15137..16477)
                     /locus_tag="IWK44_001402"
     CDS             complement(15137..16477)
                     /locus_tag="IWK44_001402"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP9549938.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16714..18237)
                     /locus_tag="IWK44_001403"
     CDS             complement(16714..18237)
                     /locus_tag="IWK44_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP9549939.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         5152 a         3902 c         3097 g         6144 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc cttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat taaataaaat
     6481 acatgtgttt tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt
     6541 tctgctagga aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac
     6601 tgtcttattt aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag
     6661 ctggaatttc gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc
     6721 gaacttcaaa gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt
     6781 gaatagttga aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag
     6841 ctgaagcaac tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca
     6901 caagagatat gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa
     6961 aagaaatatt gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag
     7021 caaggttttt accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact
     7081 gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg
     7141 gataattcgg atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa
     7201 acgttcataa gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg
     7261 tttgacagcg acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt
     7321 tggattgatt gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag
     7381 attctggttc atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt
     7441 cactgcagaa acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc
     7501 ttgttcgaaa tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac
     7561 gataataatt ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg
     7621 aatcccttct tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat
     7681 tgcacgcatc acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc
     7741 agctgtatct atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc
     7801 gtctcgggtc gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt
     7861 gatcaaagag gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc
     7921 gtcctcttct tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat
     7981 ccctagtcca tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc
     8041 atagatatcg tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt
     8101 attacttctg tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc
     8161 gctgactaca caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat
     8221 ctgatccata aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc
     8281 tcgacctaac cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc
     8341 gacaatcgag atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg
     8401 gcggccaacg attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat
     8461 ctcacttagt ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag
     8521 tatttttatt ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaaagca
     8581 gctgctttca tctcagtcac agatacaaaa tcaattattt ttcagaatca tcggttgctt
     8641 tttcttctac ttcttctttc aacgcatcac ctaaaatgtc tcccatagta aatccagtat
     8701 tttcttctgg taattcataa gactcaacat tttttggttc ttcttctgaa gctggttttt
     8761 cttctaaagc tttgatactc aatgcaatac gatgttcttc tggatggatc tctaatactt
     8821 tcactttcac ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg
     8881 aaatttgaga gatatggact agcccttcta ctccagggaa cacttcaaca aaggcaccga
     8941 agcttgtcaa acgtttaact gttccgtcta atactgtgcc aactggagct tttgtatcga
     9001 tttctgacca aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag
     9061 gattgataga caacactttg acatttacat catcattgac agccaataca tcacttggtt
     9121 ttgctacatg actatgtgaa atctctgaga catggactag accgtcaatt ccgcctaggt
     9181 cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc actgagaaca tctccctcat
     9241 ggatagttga aagaatttct tttttctttt cttctctttc gttttccact accgctttat
     9301 gcgataagat caaacgattt tctgatggtt cgatttcgat gattttaaag ctcaatgttt
     9361 tgcctttata gtctgaaaag tctgcaacaa aatgatcttc caccattgat gcagggacaa
     9421 aaccacgtac accgacgtca acgactaaac cgcctttgac aacatttgta acaggtgctt
     9481 cgatgacctt gccctcttgg aaatctcttt cgatatcttc ccaaactttt ttagcatcta
     9541 aacggcgttt tgaaagtagg tagctgccgt tttctttatc tttgccgatc gtactgataa
     9601 cgactaaatc taatacatca ccgactttta ctacttcgct gatatcttct accggcaaag
     9661 ttgataattc ttttgccgga acgactcctt caactccagc cccttcgatt ccgacaatca
     9721 cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac atcgccaact tttacttctt
     9781 gtacgctttt cattgcgtct tccattgact ggttgttctc ttgattttga tcaaattctg
     9841 tcataacact tgtcctccta accaacattc tgccgtaagt acggtactgt ttttagttta
     9901 attgattatt caaaaaaaat aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt
     9961 ttcatttttt ttcttaattt aaaaaagaag ataacctttt ttcaaaacaa cagcttttat
    10021 tgctgcaacg acttctggaa tcgatttgcc agtcgtatcg atacgaacag catcctctgc
    10081 ttgcttcagc ggagagacgt cccttgttga atcataatgg tctctttcct gtatctcttt
    10141 cgtcagtgtt tcaagatcag tcggaattcc tttttcttgg ttctctttga aacgtctttc
    10201 tgcacgctca cttacgcttg ctactaaaaa gattttcact tctgcattcg gtaatacagc
    10261 tgtcccgatg tctcttccgt ccatgacaac gccaccgcgt tcccctattt ttcgttggac
    10321 agcaactaat tcttctctga cttttccatg tgcagagact tcagaaacag cttttgtgac
    10381 atctggttga cggatttcta aagtcacatc ttttgatgct atgaatacat gctgtccatc
    10441 ttcttgttgc gaaaaagaga taggatactt tttgatcagc gaaactagcc cttcttcatc
    10501 tgaaaaagat acattgttcg taatagctaa ataagttact gcgcgataca tggcaccggt
    10561 atctgtataa atataattca attcttttgc taatatcttg gcaacggtac ttttcccaga
    10621 tgaagcaggc ccgtcgattg caatactgat ttgtgtcatg atataactcc tttttctcag
    10681 aaaaaaagcc gcaaagcgcg gctattatta tttaattctt aattcttgac cagggtaaag
    10741 cataaagttg tttggatcta agccattcaa ttcaaacaat tgatcagtgg tgatccctgc
    10801 acgctcagcc acttgttttg gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc
    10861 agctgatgaa gaaggtgttg tttctgctgc attgctgctt gaatcccctg cttgataagt
    10921 tgcctcactt gaggaagtcg gtgttgtctc tgctgcaata ctgctttctg acgttacacc
    10981 tgtatcttga gacacagtag attcttccgc gattgtacta gattctactg ttgaagtctc
    11041 agttgactcc tcttttgttg tacttgtaga actttctact actgatgtcg aagtttcaga
    11101 agaagttgct gctgttgcat tgttgcttga tccatttctg atccagaaat aagcacctgc
    11161 tggaatagcg atacataaga ccagtaaaat caaaaatagc gaaagaaata atgtatttcc
    11221 tttcttttgt tgtctctgag aacttcttga tgaaacttca tcatctgtat cgtagattgg
    11281 ttgttcccat ggttcttgtg tttcgttatt ttctgtagat tgatgtctat cttttctgct
    11341 cactgtttta cctcctaaac tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc
    11401 ctaaaaaatc aatgttccat gtttttttta aataaattta gtaaaatcgt ttcccaatga
    11461 agttgttcag tcttagaaaa aagcttcctc tttggcagaa gttctgcttt tgctccatca
    11521 atatcacaac aattctgcgg cttttcggag agtttttcgc caaaataagc taagatgaat
    11581 tttctgcggc agttttcttc attaatatag cgaagcatct gctgcaggcg gaattctttt
    11641 tctttttcct gacgtttgag ccgttccacc caattttctg gtttttcaga ctgctggatc
    11701 agctccatcc attttttttg cagctcatca aagggagctt gctttgtggc atattccaaa
    11761 taaagctcaa aactttgtcg ctgttctcta gaaagttgat taaagaaata atggatacgt
    11821 tcatccccac tttgataaaa aagaattgct gcactttctt cctgatctct gcctgctcga
    11881 ccgatttctt gtacatagtt ttctagactg tctggcaaat cataatggac cacataacgg
    11941 atatcagatt tatcgatccc cataccaaat gcattcgtag caatgagaaa ctgcagctga
    12001 ttttttacaa actgctgttg aagttgtctt ctttgagctg catcaagacc accgtgatag
    12061 tatccaactg taaaacgttc cctgaataaa tgatacaatc gttcgacttc tttttttgtt
    12121 gcacaataaa tgattgccgc tccttgtgct ctttccatga agtgttcaag ttcttgttct
    12181 ttttgctgcg ttttccgaac aaaaagcgag atattttgcc gattgactga ttgtctgatc
    12241 tcttttggtg gtcgatcaaa tagtacatga cgtatatctc ttgctactaa atctgttgct
    12301 gtcgccgtta gtgccagtgt gaccgggttt cctaaaagct ttcggatttt tcctaactgt
    12361 tgatattctg gacggaaatc aactccccat tgagacacgc aatgagcctc atctactaca
    12421 tataacgcaa tattttgttt tttcagatgc tctaaaagag aaggattagt caacatttcg
    12481 ggactcaaaa acaaaaattt atattgcgat agatgacgga ggacgtatct tctttcagat
    12541 tgcgaaagta aactattgaa agcagctact cgtttctccc ctcgtttttg taattgcgtc
    12601 acttgatctt ccattagaga aagcagcggt gacacgatga tgaccagacc ttccattaaa
    12661 taccccgtca attgatagca aaggcttttt cccgttcctg taggcaaaat agcgagagta
    12721 tcttctccat caagcaacgc ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat
    12781 ccaaaatatt tttttaattc atttattaac attctgtatc gcttcctttc tcaatagata
    12841 gatttgatac aaacgaaatt ctccataatc taattgtcca tctacattcc attcgctaaa
    12901 gcgccactct cgcacggaat ccttatttgg ttccaaacgt tcgattgttt ctgcagagag
    12961 aatccactcg tatggaaaat catcaaaaaa caaggcccac tcaataagat gatctgttac
    13021 tgttcctctt tttaaatgac ggattgccat gatctggtca attgtctgac cattcataaa
    13081 catctgcttg gtgatcatca tgctttgatt gaaattttgc tgtaataatg aatcgatcaa
    13141 agcgtgccaa agcgaatctg gccgtttttc gatttgtgct aacaaaaggt ggatacatct
    13201 gctttgttgc agataaacct ctgactcatc attgtttttt gccagctgat aaggaagcag
    13261 ccctgttcgc tcatttccag ataattggtt agctaaaaaa tctgctgctt cttctggtat
    13321 ctccgaaaac agctgcacca aactctcata cgcagtatca atcaaaattt cacgtggcaa
    13381 ttggctttca ctaagccatt tttttagttg gaaagtataa aaaggacttg tctctgctgg
    13441 aagatagtct tgaatacccg ttgccagatt agaaattact tgaacagcaa atttaatgag
    13501 ccgccaacta gtttcccctg ttctgccata gcggtcaaaa cgcaaaccag tatgttctat
    13561 ttgatgttcg gacaacatat cagcccctgc aggcgttagc tttgcttcat tttcatttat
    13621 ttcgatccaa ccttgctgcg caattttttg caaaatttga taaaacttat cctgcttcaa
    13681 ttctggaaaa ctgttatgga taaacaaaag ttcgtgggta aatccataaa taagtactga
    13741 agtcgttcgt tttcccacaa ggagttggta aagagaacta atccgcagct tgtgtcctga
    13801 tgaaaatagc gataaaataa aaacaatcat attattctcc ttaagttagg atggttagaa
    13861 atgtcactac tatgtaaact tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc
    13921 gaagcgccag atattttcga ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca
    13981 gaggctcatc aacagttcgt gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa
    14041 aaatgtccag ttcgagcgat tttattagaa aataaaatat aaatataaac gaaactttaa
    14101 aaacgtacac taagttctct aaacttgaaa ataagccggg atctgtttct cttgttccgt
    14161 gaaatcccga cttattttga aagattcatt tgctatctat agttaattga ctagtttttt
    14221 cacggaccct ataggaccca tttatattat ttcaactttt tcagccagtt ttccaatgta
    14281 ttgacgatca cagtttgctg gtctgcgtta gaaatttctg cctctccatc tcctttttgc
    14341 tcaccatagc tgccaaaacc tccatgattt ccgccattga ttgatacata ctctgttctt
    14401 tccggtaaat actgcttagc ttcttgataa gcttcctgat tcaatacttt atcttttgtc
    14461 gccgtgatcg ataaaacagg aacagctgat tctgtcaatg ttcctttttt gtcgggataa
    14521 cttgccatga agaatacccc ttttaaatgt ttgtctgaat gattcttatt gacataacga
    14581 cttgccatta cgccacctaa tgaatggcca ccgatcacat aatcggaatt tggaaaatct
    14641 tctttgacct tatccgcttt gtttccgccc atcactgcta gatcaagtgg cattttgaca
    14701 atagcgactg aaaagccgtt ttttgctaaa tcttgcgccc aaatactata actttctggc
    14761 tctacgagag caccaggata aaaaataagt aatggagact ttccatgatt ctttttctct
    14821 ccttcaaaaa ataagtaatc ttctttgttt tttccttctt ttaccaacac ttgcgcttct
    14881 gacgatggtt cgtacgtatt ttgctgtata tagtaataac cggctccacc gagtaatcct
    14941 aaaatgatga ctaagccaat tagtattcgt ctaaatattt tcactttctc ccaccttttc
    15001 tatctgagtg accccttttt cttaatcaag tatgattata ccttaaaaaa ataagagaga
    15061 aatgcaaact ttccagcatc aaataaaaaa agacggaata tccatttgaa tatccgccct
    15121 ttttgtatgt ttatttttat ttagcacata aacgatacac agcttctgca taaatttcca
    15181 ttgctttata catactactt aattcccatc gttcattcgc ctggtgcata aagtcaggag
    15241 tatcagggaa catcgcaccg aatgccacac actggttcat cgtacgagca aaagtagcgc
    15301 cgcctgatac aaacggttca gtcatgtctc cagtaatatc gcgatatgtc cccaatagat
    15361 ttttcactaa ctcactgtct agtggcacgt ataatgaatc aaggaagtca aattcttcat
    15421 aagtcaaccc atatttttct gctgttttag ttaatttagc tactaaatca tctttcttga
    15481 atgtaacagg gattctcata tctacaccga tttttgtttg ttctggtgtg atttccaaac
    15541 ttgcaaagtt catcgttagc tcgccggatt gttcatcttc tgttttgccc acgacagctt
    15601 caccagttgc attttcttga actaattttc ctaagaaatt gattgggcca aaatcaaata
    15661 cttctgaaag agcaattgcc agacgagaaa tagcattcac tccttgtgct gcatctttcg
    15721 cgtggatact tttaccaaga acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt
    15781 gttttttcaa tgcttctttt acttcagaaa gcttttctcc agagtaagga gctgcatcag
    15841 gaacaacatt caacgctcct ccagctttta cagaaaactc gttcgttcct ggtcctgtca
    15901 agtaagcttg caataagcct ttttctgcat aaattaaagg aaattcagca tctggcgcaa
    15961 atccttgcgt aattccttct tctttttcgt tatatttttc taaacaacgc cataaatttt
    16021 cttcatctgt accaaagata aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca
    16081 aagctttgac ggcatacatt gccgcaatag aaggaccttt gtcatcttgg gatccgcgac
    16141 caactaacca gccgtctttt atagttggat cgaatggttt tgtttcccaa ttattttcgt
    16201 cccctgctgg aacaacgtcc atatgacaca agataccgaa taattcatct ccagagccaa
    16261 tctctgaata accgtaatag ccttctggat ctttaaatgt tctaaatcct agcttttcac
    16321 tgatttccaa tactttatcc aaagcttcaa tcactttttt accgaaggga tgtccttgtc
    16381 ctgaatctgc ttcatctagt acggaaggaa ttcggatcaa ttcgctcaat gattctaatg
    16441 cttgttcatg atgttgttct gtcacaaatt ttttcatgat attatcctcc taatatgtta
    16501 aaaagggtca tgtcaaattc ctccgacaca acctcttctt ctcaaataat gacgttttaa
    16561 gcctgctcca tcagcaagca gaatgttctt aacaaatgat gaaagaaaaa acactcactg
    16621 aatggagcta ttgacttgtc cacgtgctta tcgatagccg tttatcggtt tcttcattct
    16681 tatgatagac cttgtacctg tatttgtcta ggtttagaac attgctgcta cacctaggat
    16741 cacacagatg gctacaaata atatcgcaat taatttaccg gtaaatttcc accacactga
    16801 aacatcgaaa cgagcaattg ctacagcacc cattacgata gcagatgttg gtgtaattaa
    16861 gtttactaaa ccagatgcag attgataagc agtgattact aaatccttac cgacgccggc
    16921 aaattcaccc ataggtccca tgattcccat tgttgcagca gctaagccgg aagttgaagg
    16981 aatcaagaat gacataggga tatagaaaat aaatgtaata atgatgaata cacttgaaga
    17041 taatgaactt aatccttgtt cgccccaatg taagatggtg tctgtgatta atccattgtt
    17101 cataaccact tggataccac gagcaacagc tactacgatt gctacaccga tcaaatcaga
    17161 agcaccagct aaaaattcag agacaaattc agactctttc atgccataag tgattgctac
    17221 tacgatggac attactaaga ataacatagt aatttctggg aagtaccagt cacctaatgg
    17281 aagcatgctt ttacctagaa ccgtacctag taccggaagg tttgttaacc agtttgtcaa
    17341 tgattcaaaa atcgtgaaat tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat
    17401 gataccaaaa gtaataaaga acaatacatt aacatgtttt tgttttttcg tgattacttc
    17461 ttgacgaccg attgcttcaa tatcgaaatg cttcatatct tctttacgtt ggttgtatac
    17521 taatgatttt gttggatctt tttcgatctt tgatgcataa cgatacacat atacaataga
    17581 aatagctaac aaaataacga acatcaagaa tcgaagacca ataccttcac ccatcgaaat
    17641 cccaacagct tgtgaagcta cgcctgttgc aaaaggattt accgttgaag ctagacagcc
    17701 tacttgagac cctactaata cgattgctac agctactact gtatcaaagc ctacagccat
    17761 catgactggg atcagtaaag catagaatgg aatcgtttct tctgccattc cgtaagtaga
    17821 gccgcctaat gcaaataaca gcatcaatac tggaataagc attttttctt ttcctttgaa
    17881 tttcttcaca actgaagcaa tcccggcatc tagtgcccct gttttgttga taatacctaa
    17941 aaatccacca acaaccaaaa tgaagaaaga gattgggatt gcagcaggtg tttctttagt
    18001 tagctccccc gctggtacac ctagcatccc attgattggc gccatgaaaa tatcccacag
    18061 cccttgagga ttttgttgta ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc
    18121 tacactatag tgcccagctg gtataaacca agtaagtaca gcgattactg caataataat
    18181 aaataagacc gtatacgctg aaggcatttg aaattttttc ttttttgttt gttccatctt
    18241 cttcttcctt tcttttccgt tagagacaac aattttggct ctaatgttat tttat
//
LOCUS       DACDSY010000051        18202 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDSY010000051 DACDSY010000000
VERSION     DACDSY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.051"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            149..472
                     /locus_tag="IWK44_001404"
     CDS             149..472
                     /locus_tag="IWK44_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9549940.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            654..2024
                     /locus_tag="IWK44_001405"
     CDS             654..2024
                     /locus_tag="IWK44_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP9549941.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(2154..3455)
                     /locus_tag="IWK44_001406"
     CDS             complement(2154..3455)
                     /locus_tag="IWK44_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9549942.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(3809..5800)
                     /locus_tag="IWK44_001407"
     CDS             complement(3809..5800)
                     /locus_tag="IWK44_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP9549943.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(5944..6210)
                     /locus_tag="IWK44_001408"
     CDS             complement(5944..6210)
                     /locus_tag="IWK44_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9549944.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(6720..8846)
                     /gene="pnp"
                     /locus_tag="IWK44_001409"
     CDS             complement(6720..8846)
                     /gene="pnp"
                     /locus_tag="IWK44_001409"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP9549945.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(9034..9303)
                     /gene="rpsO"
                     /locus_tag="IWK44_001410"
     CDS             complement(9034..9303)
                     /gene="rpsO"
                     /locus_tag="IWK44_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP9549946.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            9554..10117
                     /locus_tag="IWK44_001411"
     CDS             9554..10117
                     /locus_tag="IWK44_001411"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9549947.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(10370..10891)
                     /locus_tag="IWK44_001412"
     CDS             complement(10370..10891)
                     /locus_tag="IWK44_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP9549948.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(10894..11736)
                     /locus_tag="IWK44_001413"
     CDS             complement(10894..11736)
                     /locus_tag="IWK44_001413"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP9549949.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(11729..12454)
                     /locus_tag="IWK44_001414"
     CDS             complement(11729..12454)
                     /locus_tag="IWK44_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP9549950.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            12630..12926
                     /locus_tag="IWK44_001415"
     CDS             12630..12926
                     /locus_tag="IWK44_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549951.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            13494..15086
                     /locus_tag="IWK44_001416"
     CDS             13494..15086
                     /locus_tag="IWK44_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9549952.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            15099..15839
                     /locus_tag="IWK44_001417"
     CDS             15099..15839
                     /locus_tag="IWK44_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9549953.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            16007..17356
                     /locus_tag="IWK44_001418"
     CDS             16007..17356
                     /locus_tag="IWK44_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9549954.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(17484..18074)
                     /locus_tag="IWK44_001419"
     CDS             complement(17484..18074)
                     /locus_tag="IWK44_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9549955.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         5235 a         3698 c         3496 g         5773 t
ORIGIN      
        1 tttatacttt gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa
       61 tgaaccctcg ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac
      121 attcgttgat tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg
      181 ttacgcttca gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt
      241 tattattgac ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt
      301 gatcgaaaat cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat
      361 ggatattgat ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga
      421 tgacggtacg agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag
      481 ataaacgtgg atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat
      541 tcttggcttc tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag
      601 gataaacgct atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg
      661 atttaacaaa aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg
      721 gaaatgtttt tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag
      781 gtaaaaatgc gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct
      841 ttgcccaagg tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag
      901 attatcgggg tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg
      961 tgattttaac tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc
     1021 ctcctgaaat acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg
     1081 catcagtcag tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac
     1141 cactattctt tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta
     1201 tctattttgg catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt
     1261 ccagcgtact gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa
     1321 aagatttcaa atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg
     1381 cgtttcaatc ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc
     1441 tagggacaga tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc
     1501 agccgatgat gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga
     1561 aagaatatgg ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta
     1621 gtctcttagc aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta
     1681 gcccatcaga aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc
     1741 tttactggat cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa
     1801 gcaagatccc tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg
     1861 ttcttacagg aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg
     1921 gttctgttgc cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg
     1981 gtgaagagga acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa
     2041 tcgttgcggc taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag
     2101 gttttttaga taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct
     2161 gtttttcttt ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat
     2221 accgtgaaac ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg
     2281 ctagaagcaa agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca
     2341 tgaaaataac gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct
     2401 gactgttccg ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt
     2461 tcttctaaaa tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag
     2521 atagcaatat gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt
     2581 tcagttgtta cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa
     2641 gattgcttga tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag
     2701 ataaccggat agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt
     2761 tctgaagctt cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta
     2821 tcatcaaatt caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt
     2881 tcataagaat tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca
     2941 gatacgagac gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa
     3001 ataaaaggaa acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc
     3061 ttctccgtat tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata
     3121 tacgtaacta aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa
     3181 ggtagtttac cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg
     3241 atgatcaaac cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg
     3301 tgtgtaacat gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat
     3361 cttgatcgtc tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca
     3421 atcatcaaaa ataaaataat gaaattgata accatattct cccgcctttc tctttttcta
     3481 ttgtaccctt tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa
     3541 aggagatgcg gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg
     3601 ttgctaaaca gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg
     3661 gcattaagcc caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg
     3721 cttgttgcag tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc
     3781 gacattagtt caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag
     3841 tatttccaat gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc
     3901 atgtttctgg cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt
     3961 gccattttcc tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat
     4021 cgccaacttc tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt
     4081 gtttcatcaa atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga
     4141 aacctaagaa cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt
     4201 ctttcgtata tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt
     4261 taggccgttt atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat
     4321 caggaaccaa gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt
     4381 ctctaagctg cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa
     4441 tgttcccttg ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta
     4501 caaaaccgcc tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac
     4561 cgaagaaaaa ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa
     4621 agtaaagcag tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt
     4681 ccatatgatg agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca
     4741 gattgactgc tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc
     4801 gaggcagcag tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg
     4861 cttgcgaagc aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt
     4921 gaggcatcat ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat
     4981 aaacattcaa caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt
     5041 aaggccagct cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct
     5101 ctgcacctgt cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg
     5161 cactaaaaag cagctggata gcataataag gattcagtgc acggataact gtccagtctt
     5221 gactgaagtt cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc
     5281 cgaatgcttt tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa
     5341 taactaaagt gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg
     5401 gaattcctcg caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat
     5461 ctgctagtaa ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc
     5521 ggacaagtgt gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa
     5581 tcacgacata tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc
     5641 cgataacaaa atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca
     5701 taacgtaaag cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc
     5761 cagtcattga tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt
     5821 tatatggata tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg
     5881 ctttcctaaa ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata
     5941 aaattattct ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag
     6001 gcaaaaaatt gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag
     6061 tggtcctagt aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga
     6121 tacgcctccc gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc
     6181 gataaataac gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca
     6241 cttttatttt acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa
     6301 aaaagaagtt gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg
     6361 tgaatcagag gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa
     6421 tagttcctca tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg
     6481 tttattggag aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa
     6541 aacagctcct aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac
     6601 accgtttatt cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga
     6661 ataattattg tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat
     6721 tatttttctt catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt
     6781 acgcgtcctt gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca
     6841 acatcttcga ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct
     6901 tttcctttga tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga
     6961 taaacttcac caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg
     7021 atcatttctg catcagctga agcaatactt acttttccat cttgatcgat atcgattttc
     7081 acaccagttt catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg
     7141 attttagcag gttcgatctg gatcatttcg atttttggtg catattggct taattcttca
     7201 cgtggagcag caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct
     7261 tgagttaatg cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt
     7321 aaagcagtga taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct
     7381 tccagccctt ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc
     7441 gcaataccgg cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca
     7501 gcacagatac ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg
     7561 atcgtataag gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat
     7621 gcgccatgtc cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa
     7681 aattgaggga aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca
     7741 atgatttggt gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct
     7801 cgtgtgaaca aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga
     7861 cggatttcat ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt
     7921 acgacatctt tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca
     7981 tcttcatgac ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg
     8041 atattatctt cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa
     8101 gaagcatcaa agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt
     8161 ttttctttcc ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg
     8221 tcaaaaccaa acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt
     8281 tcaaccatat tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct
     8341 gcttgttcta ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct
     8401 gcgattgggc cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt
     8461 gctgccatag ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact
     8521 tcattacgga agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc
     8581 aatgttgctt ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct
     8641 gctgcataca ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct
     8701 tctttagagg ccacagctgc acttaagact actgtatctc cataacggac taaaacggct
     8761 ccgtttgctt gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc
     8821 gttttgaata cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg
     8881 ctactaaaca aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta
     8941 ggagtccatc tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga
     9001 gattcatgtg aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat
     9061 caattcgcgg taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg
     9121 accaactttt ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg
     9181 ttcattcaag tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt
     9241 atccccttca tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc
     9301 catttctttc acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct
     9361 taaccaagtg gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt
     9421 gaaaatcaag tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt
     9481 tccatttttg tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag
     9541 gggtacatat tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg
     9601 cgaagttgca aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga
     9661 gatgcttgag ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg
     9721 aggcggtgtt ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca
     9781 cgtaccaagt cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc
     9841 taaaattttg agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc
     9901 tgttgatcga gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta
     9961 tgatatgaat ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca
    10021 acatgaaatc gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc
    10081 atttgcacta aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata
    10141 ctatgctaca agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt
    10201 tatgtttctc cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt
    10261 ctgtgtattg gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat
    10321 ctcagagatg atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt
    10381 agaggattcc tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt
    10441 agcggatcgt atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt
    10501 tttgataacg tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg
    10561 ctaactcacc gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa
    10621 cgattagtct cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga
    10681 tgtgcccagt attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac
    10741 ttttacctgc agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa
    10801 aagttttcgc aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga
    10861 tttgttttcg ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta
    10921 ttcgcgcgag tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt
    10981 atttttcagc agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac
    11041 tgcttggttt tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc
    11101 taataaagca accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga
    11161 ctcttctgtt agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac
    11221 tacattgtcg ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc
    11281 tagagtgaca cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt
    11341 aatgggtttt gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc
    11401 cgtgtaaagc tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac
    11461 atctaaaaat gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc
    11521 gtaatcaaaa ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc
    11581 tttcaacaat tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact
    11641 tcttgcaaac atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc
    11701 aatcattttt tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt
    11761 ttttgaaaac aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag
    11821 gcgtttgatt gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa
    11881 acggatgaac ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat
    11941 aagccgcctg ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc
    12001 gtatttgtcc tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg
    12061 tttgttgaca ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg
    12121 ggcttttagc ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt
    12181 ctttcgctag atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa
    12241 tctttttgac aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca
    12301 agtattccgg ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc
    12361 cagaaggtgt ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct
    12421 cttttgctgc ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata
    12481 gcccatactc gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta
    12541 tgggcatttg agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat
    12601 actgctgtat aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta
    12661 ttctggcttg tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac
    12721 gcaaagaaaa tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt
    12781 tagccattac attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt
    12841 tgatttatct aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac
    12901 gtgagccaaa gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc
    12961 tgttctagtt ctttttttat tgtattacat acatttttgt gtataattat actatcgttt
    13021 gcaatggata gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat
    13081 ttatcagtat tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta
    13141 aacttttttt gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg
    13201 agaatcatta accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc
    13261 gaaatgtgca cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact
    13321 gtcttagcag tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat
    13381 tttagcggcc tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta
    13441 ccgcatttca tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa
    13501 aaaatatggt ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat
    13561 ctctggcaaa tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg
    13621 ggtatgcacc cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag
    13681 gccaaaatag ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt
    13741 tagggaaaaa attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat
    13801 caggcaagat cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa
    13861 ttgattttac cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca
    13921 aatatgcaaa agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa
    13981 atacattcca ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg
    14041 ataatttctc agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg
    14101 aacgtccgga aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca
    14161 gcggtgcaga tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta
    14221 aaggcgaccc ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac
    14281 gaaccaaaat tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa
    14341 aacctggcat catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac
    14401 gaggtgctgg acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg
    14461 gattactgat tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct
    14521 tccaaaaaat cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga
    14581 tgatggtaca agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga
    14641 accgaacgat tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga
    14701 tcgttcgtgg ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg
    14761 gtatgaccca tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct
    14821 tgccagcaac aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa
    14881 tcggcgtagc tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct
    14941 tccaaacatt tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa
    15001 tcctgcgggc ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa
    15061 tcgacaattc aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa
    15121 tcaaacatct aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag
    15181 tcaataaagg tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct
    15241 tgcgctgtat caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa
    15301 atgtattagc tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc
    15361 aatccttcaa cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa
    15421 ctgtcctgaa acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag
    15481 ttggtatgga tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc
    15541 gtgtcgcaat tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta
    15601 cttcggcgtt agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac
    15661 atactggctt gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg
    15721 accgtgtcat ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct
    15781 ttgtccatcc gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg
    15841 atcaatagct gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta
    15901 ctcataaaag gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct
    15961 cttaatatgg aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca
    16021 agaactgcag cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat
    16081 tggaacgggg ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt
    16141 gctcgcctat cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact
    16201 tctgttatca aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa
    16261 aaaagcggct ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc
    16321 cgatttaaca gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct
    16381 tcctgaactg attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt
    16441 tggtgagtta gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct
    16501 cgtaggagct tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac
    16561 gaatctatgg tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt
    16621 ccaaatggta acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac
    16681 agaaaatcct gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt
    16741 attttactta ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc
    16801 agataacagt ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt
    16861 gatcaattta gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac
    16921 aggacgtatg cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt
    16981 aacaacacat catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc
    17041 tgttatcttg aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac
    17101 tacttgtttc ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag
    17161 tatcctcggc aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct
    17221 ggtatttctt tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat
    17281 cgctttatta ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata
    17341 tgaacgtaag aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag
    17401 gaatttacga aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa
    17461 aagcttctgt cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct
    17521 gcttttttat aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc
    17581 aacggtacaa aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt
    17641 cgacggtcct ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt
    17701 tgcctcatca agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc
    17761 tcaggccaaa aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc
    17821 agccaaaaga gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc
    17881 tgaaatatac gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa
    17941 gctaataatg tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg
    18001 atctctaata caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat
    18061 ctcgactttg tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac
    18121 gttattaaat aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg
    18181 aaattttgtt acttttaaaa aa
//
LOCUS       DACDSY010000052        18116 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDSY010000052 DACDSY010000000
VERSION     DACDSY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.052"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            62..403
                     /locus_tag="IWK44_001420"
     CDS             62..403
                     /locus_tag="IWK44_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9549956.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            712..1755
                     /gene="pheS"
                     /locus_tag="IWK44_001421"
     CDS             712..1755
                     /gene="pheS"
                     /locus_tag="IWK44_001421"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP9549957.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            1760..4180
                     /locus_tag="IWK44_001422"
     CDS             1760..4180
                     /locus_tag="IWK44_001422"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP9549958.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(4350..5360)
                     /gene="trpS"
                     /locus_tag="IWK44_001423"
     CDS             complement(4350..5360)
                     /gene="trpS"
                     /locus_tag="IWK44_001423"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP9549959.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            5614..6351
                     /locus_tag="IWK44_001424"
     CDS             5614..6351
                     /locus_tag="IWK44_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9549960.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            6365..7180
                     /locus_tag="IWK44_001425"
     CDS             6365..7180
                     /locus_tag="IWK44_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9549961.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            7193..7840
                     /locus_tag="IWK44_001426"
     CDS             7193..7840
                     /locus_tag="IWK44_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9549962.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            7856..8512
                     /locus_tag="IWK44_001427"
     CDS             7856..8512
                     /locus_tag="IWK44_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9549963.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            8674..9495
                     /gene="racE"
                     /locus_tag="IWK44_001428"
     CDS             8674..9495
                     /gene="racE"
                     /locus_tag="IWK44_001428"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP9549964.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            9498..10853
                     /gene="rph"
                     /locus_tag="IWK44_001429"
     CDS             9498..10853
                     /gene="rph"
                     /locus_tag="IWK44_001429"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP9549965.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            10854..11372
                     /locus_tag="IWK44_001430"
     CDS             10854..11372
                     /locus_tag="IWK44_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9549966.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            11479..11979
                     /locus_tag="IWK44_001431"
     CDS             11479..11979
                     /locus_tag="IWK44_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9549967.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(12259..13179)
                     /locus_tag="IWK44_001432"
     CDS             complement(12259..13179)
                     /locus_tag="IWK44_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP9549968.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            13344..14357
                     /locus_tag="IWK44_001433"
     CDS             13344..14357
                     /locus_tag="IWK44_001433"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP9549969.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(14467..15222)
                     /gene="fabI"
                     /locus_tag="IWK44_001434"
     CDS             complement(14467..15222)
                     /gene="fabI"
                     /locus_tag="IWK44_001434"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP9549970.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            15442..15882
                     /gene="fabZ"
                     /locus_tag="IWK44_001435"
     CDS             15442..15882
                     /gene="fabZ"
                     /locus_tag="IWK44_001435"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9549971.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(15997..16758)
                     /locus_tag="IWK44_001436"
     CDS             complement(15997..16758)
                     /locus_tag="IWK44_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9549972.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            16947..18041
                     /locus_tag="IWK44_001437"
     CDS             16947..18041
                     /locus_tag="IWK44_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9549973.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         5900 a         3152 c         3840 g         5224 t
ORIGIN      
        1 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
       61 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
      121 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
      181 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
      241 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
      301 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
      361 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
      421 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
      481 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
      541 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
      601 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
      661 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
      721 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
      781 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
      841 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
      901 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
      961 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     1021 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     1081 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     1141 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     1201 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     1261 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     1321 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     1381 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     1441 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     1501 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     1561 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     1621 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     1681 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     1741 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     1801 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     1861 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     1921 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     1981 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     2041 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     2101 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     2161 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     2221 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     2281 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     2341 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     2401 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     2461 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     2521 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     2581 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
     2641 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
     2701 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
     2761 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
     2821 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
     2881 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
     2941 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
     3001 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
     3061 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
     3121 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
     3181 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
     3241 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
     3301 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
     3361 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
     3421 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
     3481 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
     3541 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
     3601 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
     3661 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
     3721 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
     3781 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
     3841 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
     3901 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
     3961 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
     4021 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
     4081 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
     4141 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
     4201 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
     4261 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
     4321 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
     4381 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
     4441 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
     4501 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
     4561 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
     4621 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
     4681 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
     4741 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
     4801 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
     4861 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
     4921 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
     4981 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
     5041 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
     5101 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
     5161 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
     5221 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
     5281 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
     5341 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
     5401 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
     5461 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
     5521 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
     5581 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
     5641 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
     5701 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
     5761 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
     5821 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
     5881 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
     5941 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
     6001 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
     6061 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
     6121 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
     6181 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
     6241 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
     6301 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
     6361 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
     6421 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
     6481 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
     6541 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
     6601 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
     6661 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
     6721 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
     6781 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
     6841 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
     6901 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
     6961 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
     7021 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
     7081 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
     7141 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
     7201 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
     7261 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
     7321 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
     7381 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
     7441 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
     7501 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
     7561 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
     7621 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
     7681 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
     7741 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
     7801 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
     7861 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
     7921 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
     7981 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
     8041 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
     8101 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
     8161 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
     8221 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
     8281 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
     8341 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
     8401 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
     8461 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
     8521 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
     8581 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
     8641 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
     8701 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
     8761 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
     8821 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
     8881 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
     8941 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
     9001 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
     9061 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
     9121 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
     9181 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
     9241 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
     9301 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
     9361 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
     9421 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
     9481 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
     9541 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
     9601 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
     9661 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
     9721 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
     9781 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
     9841 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
     9901 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
     9961 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    10021 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    10081 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    10141 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    10201 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    10261 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    10321 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    10381 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    10441 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    10501 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    10561 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    10621 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    10681 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    10741 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    10801 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    10861 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    10921 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    10981 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    11041 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    11101 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    11161 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    11221 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    11281 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    11341 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    11401 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    11461 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    11521 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    11581 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    11641 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    11701 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    11761 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    11821 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    11881 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    11941 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    12001 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    12061 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    12121 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
    12181 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
    12241 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
    12301 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
    12361 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
    12421 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
    12481 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
    12541 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
    12601 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
    12661 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
    12721 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
    12781 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
    12841 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
    12901 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
    12961 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
    13021 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
    13081 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
    13141 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
    13201 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
    13261 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
    13321 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
    13381 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
    13441 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
    13501 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
    13561 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
    13621 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
    13681 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
    13741 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
    13801 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
    13861 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
    13921 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
    13981 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
    14041 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
    14101 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
    14161 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
    14221 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
    14281 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
    14341 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca
    14401 ggaagagact ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac
    14461 aaaagtttat gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct
    14521 ggaaagtggg ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg
    14581 ctgatcaggc gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt
    14641 gacagctaat gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga
    14701 gaactcagct gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat
    14761 gttataattc gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt
    14821 tggtgcaaga aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc
    14881 ttgagctaat tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt
    14941 gttggcgtaa gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc
    15001 attcattgct ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc
    15061 ttcgccgacc aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat
    15121 ttctgcccct tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc
    15181 gacgcccata atgacgattt tttttccttc tagaaatgac ataatgactc tcctttgttt
    15241 ctgataaaaa tttgatagtc gtaaaaaaat agagaataat agctaaaata acttcttgat
    15301 ttcttaatta ttagtcaaaa tgttttgatt gtcaaactat tttttcttaa attaaaaaaa
    15361 gattgctaaa tatttaggaa tatgatactt tgatagtcaa agtaatgaaa atggcaattg
    15421 cctgaaagaa ggagtttaat tatgtcttac gtattatctg ctactgaagt aatggaattg
    15481 attcctaatc gttatcctat ttgttatatt gattatgtag atactttaga accaggaaaa
    15541 aagatcgttg cgacaaaaaa tgtaacaatc aatgaagatt ttttccaagg tcactttcct
    15601 gacaatccag tgatgccggg tgcgttgatt ctagaaacac tggcacaagc aggttcgata
    15661 cttatcctaa aatcagaaga gtttctagga aaaagagctt atatcggtgg gatcaataag
    15721 gccaaattcc gtcaaaaagt tgttccagga gatgtgctga aatgtcattt tgaaatcacc
    15781 aaaatgaagg gatcagtcgg tacagcgtta tctgcagcat atgtagatga taaaaaagtc
    15841 tgtgagtgcg agttcacgtt catcgtcaac caaaatgctt gactataaaa cctgatatag
    15901 caaaacaaaa ggaagcttac ttgcttttta tgtaaaaaga agacgcctga atcctagcat
    15961 acgttgtcat ctatgcagga ttcagacgtt atttttttat ttttgttttg cttcttgcag
    16021 tatattgatt ttatcgattt ttacttcttc ttttggtttg tctgaactat ctgtttcgat
    16081 atttgcaata gcatccacga catccattcc ttcaatcact tggccgaaga cagtataatg
    16141 gccatctagt gaaggataac cgccgttttt ataagcgtcg atgattgctt gaggatagtc
    16201 gtcttttaaa agaccatcat gcatatcatc tgtattttga acgataaaga actggctgcc
    16261 gttactgtta ggatcttgtg agcgagccat agataacgca cctcttatat tataaaggtg
    16321 gttgttgatc tctgtttcaa atccttctcc ccaaatgctt tcccctccag tgccatcgcc
    16381 tttaggatcg ccaccttgga tcatgaaatc tttgatgacg cgatggaaag tcagtccgtt
    16441 gtagtaaccg tcttttgcat gtgtcataaa gttttcaacc gcttttggtg cgtgttcggg
    16501 aaataatttg attttgattg tgcctttatt cgtcaccatt tcgaccagat cttcattttc
    16561 ggcgacatct ttcgtcaatt gtggtaattc caatgcgttc aaatcgacgc tactggcagt
    16621 cgtgctttcc gcagtagatg tactttttga actttctgta gacgaactag aagaagtcgt
    16681 gttgtttgca gaacaaccag cagcaaacag caatgctgct gctaaactaa tacttaaaat
    16741 aactttattt ttcttcataa ctgttccttt ccaatttact attttgttat ctttaatgat
    16801 aacaaaatag agggaaaata cctacttaaa ttattggaat ttatcattta ttttaaaaga
    16861 aaaagaaaag gtgtagacca tttactttgt gtttgctata atgtaagtgg atacaaaaac
    16921 gaaaagatgc aggaggaaat ggtaatatgg gaaaattagg aatctcgatt tatccagaac
    16981 ggtcaacatt tgaaaaggac aaagcgtact tggatttagc tcataagtat gggtataaac
    17041 gggtttttac cagtttgctg cagatcaatg atgataaaga aaaagttctt tcagaattta
    17101 aagaagtagt tgattacgcg aatagcctag gcatggaagt aatggttgat atcaatccag
    17161 cgttatttga acagttggag atttcttacg atgacttatc ttttttccat aagatgggtg
    17221 cctacggggt ccgcttagat atcggattca caggagcaga agaagcaaaa atgacgcgca
    17281 atccttttgg tatcaaaatc gagatcaata tgagttcggg cacgaattac gttgacaaca
    17341 tcatgagtta ttcgccaaac acggataatt tattaggttc tcataatttt tatcctcatc
    17401 gatactctgg tttaggatac gaacactttg ttttttgttc agaaaaattc agaaaatata
    17461 atttgaatac gatggcattc gtcaattcgc aatctgctga gtttggccct tggcctacgc
    17521 aagatggctt gtgcacgctg gaagaccacc gagatcttga aattgcgaca caagtgaagc
    17581 atttaatcct tacaggattg attgatgata tttctattgg aaatgcgtac gcaagtgaag
    17641 aagaattgaa agaaatggca gaagcattta acgcggatta tccaacattg aaagtagata
    17701 cggaagaagg tattacagaa aacgaacgaa tctgcttgtt tgataacctt catagttatc
    17761 gaggggatcg ctcagaatat atccttcgtt caacgatgac tcgtgtatac tacaaagata
    17821 aagatttccc gcctcataat acgcgagata tgcatcatgg ggatgtactg attgacaatg
    17881 aagggtacgg tcaatacaaa ggagaaacac aaatcgcctt gaaggatatg aaaaacgacg
    17941 gacgtgtcaa tgttgtagga agaatctccg atgacgaatt attcctcctt gactttttga
    18001 aaccatggtc aagctttaaa ttaattgaaa ataaaaaata gataattagt tattgcagaa
    18061 ataatccgct agaaatagcc agttatttct gcttttttat tttgtttttt tatagg
//
LOCUS       DACDSY010000053        18052 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDSY010000053 DACDSY010000000
VERSION     DACDSY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.053"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            196..795
                     /locus_tag="IWK44_001438"
     CDS             196..795
                     /locus_tag="IWK44_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549974.1"
                     /translation="MNLKNPNSNKSTLYVISDDYINKLKKVDYKVQNNYREQRLYFKS
                     DLKISDRNEDINYYVPLASAKENQRSLTNKSVFKIYGDREEKDFLGVLHINNMIPVPK
                     DCAKVWSPNSPSVDEKYKMLVIKQAKYLKSHEPEINESCKLLYNSKLGKLDPSYFKKN
                     RKEVITYQKIMTNVESLEPVSIAESRKNTLVQTRSIDIE"
     gene            875..1099
                     /locus_tag="IWK44_001439"
     CDS             875..1099
                     /locus_tag="IWK44_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549975.1"
                     /translation="MISMARPRKYKTEEERKEALKKARKKWVEKNQETIAYIRAKSFA
                     KKFIEMSEDEDIELLEKWLDERKNNRQNDE"
     gene            1529..1735
                     /locus_tag="IWK44_001440"
     CDS             1529..1735
                     /locus_tag="IWK44_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549976.1"
                     /translation="MIENRVLGFVECKDNIDDTLEVLESIIFNTKVLDLKAVNGDMVT
                     HIILDEMSAKEIGETLIAWAKKEL"
     gene            1813..2667
                     /locus_tag="IWK44_001441"
     CDS             1813..2667
                     /locus_tag="IWK44_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9549977.1"
                     /translation="MMKSKFLGYVIAINMFKGGVGKSTLTQLMADILSARGFKVLVID
                     VDPQGTVTKKFQRQYDLKVVGKMTLFEAVINMSFLDCISPVKENLDIVLGSEQMSRFT
                     KVLRDMWRSTGFTDEGEEVFWNLFNYMIGNDNLRDQYDFILFDTIPTVSEFTDNVYVA
                     SDFLVIPTQTEQDSIDNLHSTVTNYQTAVKEVNGDLEILGIVPYLVDTKLKTGLELLS
                     ELQEEYGDLVYSNIVSERGVIKRWGREGLNKAIPHSKIAYNMYVNIVEETLIRLEKAT
                     GEKILEHV"
     gene            2680..3117
                     /locus_tag="IWK44_001442"
     CDS             2680..3117
                     /locus_tag="IWK44_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549978.1"
                     /translation="MMSKTKKVTSAGNNGSGNPFGIKSGSGGFNIGQQMLKGKAGIKS
                     TGDKRKSVRVPVDLYDEVVKDLSYGAKYSLMTEILAAFLKEFEIHENSIVTDFGKLKT
                     RPVKISEEAFKVLKVIKFETDQSNTNIIASALTWYLDKRKGKA"
     gene            3437..3880
                     /locus_tag="IWK44_001443"
     CDS             3437..3880
                     /locus_tag="IWK44_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9549979.1"
                     /translation="MQILEDIKIYWKRIIGYTLILLGVNLVLSMIALIFVKQGQENVT
                     EIVSFTSILISGIFAPFFEETLMRGIIQNQLKVKTNLSTRNIYIIVAILFSLFHLNLY
                     FVPYLMTSLILSFVYDNSEKRLIVPVTIHCFYNLIVILLSIVFYN"
     gene            4027..4350
                     /locus_tag="IWK44_001444"
     CDS             4027..4350
                     /locus_tag="IWK44_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549980.1"
                     /translation="MARSRIDKIEQTKKDQKGFKNKTKFFKAEDVKRIAWSVKTFDED
                     RYVKVSMNGLFVQLGNFSNFKTIDELYPEIRNIVDSKGLIVAKRKSRNSGLAYCKDKG
                     IMIPA"
     gene            4768..8406
                     /locus_tag="IWK44_001445"
     CDS             4768..8406
                     /locus_tag="IWK44_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549981.1"
                     /translation="MKKLKLISVALISLITLSTARQSVVSAEGTSTAEAVVEESGDKK
                     VAIDDKVKNEAEKATVIQGADGQFYVVANESDKDKLVSSETPQIAEKTEGDFTAVEVG
                     IDEKRQEKADQADQEIKDFDEIDLDDVESLQSAVNSEDLPFIEEDQVAEELSKAKDLE
                     ESEQILMQTRFFYAEEILNQTNNLNDVPSMLKESKVALLASVSKTVSETTINPPSGYP
                     WLMYIVRSNGYSYTEDRGIRRTIDGKDAFCLQAGVTAAKTYTDGGSSYLSTSQKEMIT
                     SIISAYRELGGWTNNDAGKQRWMAAQILVWENSNEKPKSITITPNHKAGVTDWKSKIA
                     TRASQIRTRPQWDATSKTVYEGEKATFTVKNSWGDHNLYVESVSGGAKATISGNKLTV
                     DTNGSTATKITVNMVKGSKTNEKYPTTVWVNGSYQKLVTGSFYVSTSVTVNVKRKGTV
                     DVLKVDAETKKPLANATFRFTYDGKTKDVKSGTDGKAKLGERLKAGTVVKVKEITAPS
                     GYQLDSSEFSITVKENQNVTTTRTNKKSTGSVEIEKIGDLDELGLPNVVFTIYSSDNK
                     IVKDNLKTDSNGKIKVDELQFGKYYALEKQGVTGYDPDGKKYNFEITQDTSSEKPAKV
                     KVTNIYEVSVNEKVSDMNENQVLKNVWSADPNDYLQYDITTGNLKKSGERNVQSFTIE
                     GFYDNKNVDVSKSEVYVGSTNVTNSFDINNDIGNGKIIAKAKATVLTNNDFYNKSYNL
                     RITMKIKKSTLENTEKQKPLTIVKNSKITVGSKISKSVNSNDVETVLYSRKITINHID
                     EKDKHLLKQDIDYKYDGETYEYKPRIDLFDNDGNNYKSAVVHKGKIEGKDTVLTTPYH
                     IPVFSVNVDQIQIDTARAVKNGYLPTELILSKKEEYEKELEKIVFKVKLTDIDNNKVV
                     YDENFKYKEFNSHMSIDLPTEYLTKNKKINYSIDILLVENPDKNKFETETKDLRTIGY
                     TSSEKVLTNKDMTNNQVNYSAVVRTVKERASNKPVKEFIESINYKFTDNTKTKTGYGI
                     SVDLEPTYRNEIGKQIDMKVYSYPMNELIDSKIDEIYQSGRKETEVKMDRLKNDGKVE
                     SGINTQNLSFKFPHINIERKTGNIYADSQKNDESIKYELIDGGRKLYVPIWCDLGNYS
                     IAIKSSNFGGNYISLDMNQNIDVYAYMYATIDSETKKEDELLLEPVYPESTLPDGWNK
                     NEIDWLNK"
     gene            8465..8827
                     /locus_tag="IWK44_001446"
     CDS             8465..8827
                     /locus_tag="IWK44_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549982.1"
                     /translation="MRVQSYGNSVFDVKREFQDSRRKTKKLIAYFILSAIVFYIVKFV
                     LELNSMVFTDMVHKMLNGESINFNFDYMLEKAQSNQVVAFLVSTYYFIEKSIFSLKDF
                     FMGLFTKLTTFYNKILKN"
     gene            9061..9483
                     /locus_tag="IWK44_001447"
     CDS             9061..9483
                     /locus_tag="IWK44_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549983.1"
                     /translation="MIYVALFLDGSDVMLRDMPKMILLMFVGFFLILVIAYYSLNGYT
                     QDSSVKGMQETLRTTAIANRDDSIRVEKGRFALIKSDFEKDFKEKFGKSKNLNNEITD
                     YKFDYLTDGKDGIKAIKVKVESGSKEYQATCILNVAND"
     gene            9495..10907
                     /gene="cpaF"
                     /locus_tag="IWK44_001448"
     CDS             9495..10907
                     /gene="cpaF"
                     /locus_tag="IWK44_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP9549984.1"
                     /translation="MRVVEEKKISPKIKESSKSKINIASIIKKNGIKENSEAIEGNLD
                     FLTKLLEEDSGNYEPPIENAKLNMIRDDLTRNYSQEVLQALTDDEIRQNLVQVVLKEH
                     GTFFKKDKEVAEYVVRECIGTGIIERIMTNDEITDVGWNGSQLSIETNTNKWLLDGDK
                     LNITQEYIERVISKYAVVNDRDFNSGNPVMDGMFKNVRISATHIDNSPDGSTFSMRVA
                     RPRLALNKENFEDFAPDYILELFEKMMKTKANICISGETGTGKTELQKLLLSFIDNHD
                     RMIMIEDVQETHAKELFPDKDIFAWITTPKKAISDLVKASLRNNPRWIIVSETRGSEA
                     YEMLQAVLSGHYVVTTLHAINARAIPRRFVNMCASGFKINENAVEEDINRYFDFGVHI
                     KKTVVDGKVIRYLNEIVEFSADEPTTVFKQKFINGKFVTENGSLSEEFKEKMAEKFIT
                     FDFPEEQTNAEESLPEEEVI"
     gene            10924..11739
                     /locus_tag="IWK44_001449"
     CDS             10924..11739
                     /locus_tag="IWK44_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549985.1"
                     /translation="MTKRKGLMDVSELNAYQVAYGKPLDYKDYLNYVGLPCLFMGIFS
                     FALCYYWWIALICAVIGGVYGFKVIMPKSIKRSYELKSLQERNKFVNNMTQILTDDSK
                     TVTKALAIAKQRTKGELREDIQILESRLQGADKFQIAEAFREINNKYYSDVVFTQYFE
                     QLETAIYEGRNNSDTMKQIKSYHNDVKRKTEGFMRIKDGYLQDMKQMMFIVAIFIGAI
                     TVAFGFSTFYEGFARSPIGWVFGSIYYLIMVSFVKSFFKHYFDDEIMSLGVSN"
     gene            11739..12536
                     /locus_tag="IWK44_001450"
     CDS             11739..12536
                     /locus_tag="IWK44_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549986.1"
                     /translation="MKIKALKRKRKSSVSPLKITKTEHKSFWIKLLGDVYYQKLREMN
                     NSNVAIIKWQKQRLKKSLIFIVVGFLMYLFLGKEWYYLAGGIAISVGFYVWQAKSING
                     IYNQFRFERHLQFSKFTRLLIPYLKQKRDGGNLYGVFAKIVKRLDYDTDKKLLMKLMQ
                     EMTDQPNDIRPFTEYAEQTSGTDMSILFMSTVYDIRQGSADLDVIEELDRLASEELMA
                     GIDSIIDYKSRRFVFFPTKIAMTSFILVVGFAVGVLFMNLKDINFGQ"
     gene            12554..12916
                     /locus_tag="IWK44_001451"
     CDS             12554..12916
                     /locus_tag="IWK44_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549987.1"
                     /translation="MQEQSVMEIIKWFIGLIMIMTMVSIGLFCVRLQDINTFKQQVNY
                     QIERKGGLTTEAVEAIDKYSATNYDGRFTIESDKLNQKVSYGEEIDYTIKATIPIQIL
                     PLPDVSLSFKGNSVSQIR"
     gene            13088..13309
                     /locus_tag="IWK44_001452"
     CDS             13088..13309
                     /locus_tag="IWK44_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549988.1"
                     /translation="METIQENRKDNQEIIEKNKKTRKKKVDKFTEMTDKIIEKKGLKP
                     KDFDRQAIKEARQKLYDDFVKENISQLLD"
     gene            13328..13603
                     /locus_tag="IWK44_001453"
     CDS             13328..13603
                     /locus_tag="IWK44_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HAP9549989.1"
                     /translation="MKNLVISKFSMLPRMDFFDNVSKFMSNMTSKLQGISLSVIIFCI
                     VVTGLMFLFGEGPSRTAKKWLTYIVIGAVIIFGASTIGQTIKSVGGF"
     gene            13712..15538
                     /locus_tag="IWK44_001454"
     CDS             13712..15538
                     /locus_tag="IWK44_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549990.1"
                     /translation="MNQRFEYNFEDVEPKLLKKGKGLKKKNKYTLKQAEALVGWIQHN
                     WSDKTTITLICSDTEEGTKYAFDGIEITPFSIGGQEKLLFSDIEGQILQAPDSMISDP
                     NKLILDINDQYKHEPDVAEPTKESKSKLNLKLPSVVLPFAKKKNKLMNELIEPEETDI
                     YEEELEEQEEIEELVVSSEETEEVEEIETVSENKEEIEEIFDFEDEEEVENVKKDGST
                     TSSQNGLVAVNDPQVPPYVPSQAPNYTPQYQAPNVLQPIRKHETVQLVTLKEYCDLGE
                     EIETSISQMVEKLKPESLFKFIGIPSTGLTNTRIDEYRSNHAMLRLAEVKFENLREHY
                     SRQIISFKAEALDRLRTAVDIAWKKPYDLDVKKDKAEELSKMEVEAEDKLNEFVKRQE
                     NNVNDKIKKFEIEQEIDLKNFIARQEADKAVFIATEKDRTQNLIDSKAESINADLLEA
                     KDKFIDEEMYKMKIEMNKNLFDGKQKIKSELATELAKANDNVWENALKFIQEIQEEIE
                     TKTPIWAAEIKEFNSLEEQEHKRMKQTKELELKEESIEIKRLEAEMNLKRMEELDHEN
                     KMLKIKLETALSKNELLAMEQDTKQVVNGSNRVGALNLFSKR"
     gene            15551..16264
                     /locus_tag="IWK44_001455"
     CDS             15551..16264
                     /locus_tag="IWK44_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9549991.1"
                     /translation="MSVFNNKVMLGNCYVNHVGFYDGGTYRVLFLRLSVFSYSKDGKG
                     YYENVNAQISCPVDRNGKPSTTSSHPKALMNKFIYEKLDRNLVEGMKISIHGNIRGTE
                     QVLLNGGWKNFGQLSSLEKEEYQKIPKNELVTRNQTYIYIEEVSMPEKEEFRKTQSKA
                     TKEDVEVVEPNQADEILSDDADPVNDEGEVIEVFEAEIIEEEEVKSVDKRMYREKSKG
                     YVNFEDMYYDEYSNEPPIN"
     gene            16276..>18052
                     /locus_tag="IWK44_001456"
     CDS             16276..>18052
                     /locus_tag="IWK44_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP9549992.1"
                     /translation="MKLVQNSEVKKRKNGFAKNSNKILVKLKKAGSLKKNKYHTGKIE
                     GEDLPKSLQDKLNVPLSFLGIFLGSIVFLAITYLLNFLKSLLVAIGQSESGLLGVEFD
                     PKFSLTHLRPLGDTKQYFMCAVVVVIGVAYLIYGKLNHESEKNLVYGQKGDSRLSTIK
                     EIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGKGETFVVPL
                     LDNLSRAKIQSNMVVNDPKGELFSASKETLERRGYRVLVLNIDDPLESMSFNPLQLVI
                     DSWANKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDC
                     IEKITMYNVAQMLNELGSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGD
                     KAKGSILSTASQGIEMFTSDLFGKLTSKMSIDLKEIGFPKSIQFKVNTNLVGKRISIS
                     FLRRENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLDIRYEEEKSRAWYRIEFPK
                     KQANSHDTSLVTKKFKSGANFSDLEISTLRLKYTDQPTAVFMVIPDYDPSNHVLASIF
                     ISQLYTELASNCKQTPDKKCFRRVHF"
BASE COUNT         6835 a         2291 c         3504 g         5422 t
ORIGIN      
        1 tgtagtgctg ttatattgaa tgagaaggta aataatacag ttttgatatg tatagtgtat
       61 ttcaccagta aattggcata aactcgaata taacttcatg aattaagtgt aaaataagtg
      121 caattagttt gaaaatagat tagtgcctgt taatcttaac atataatata caatccattg
      181 gaaaggggag atccaatgaa tctgaagaat ccaaattcaa acaaatcaac gctctatgta
      241 atttcagatg attatataaa taagctaaaa aaggtagatt ataaagttca aaacaattat
      301 cgagaacaac ggttatattt caaatcagac ttgaaaattt cagacagaaa tgaagatatt
      361 aattattatg ttccattggc ttcagcaaaa gaaaatcaac gttctttgac taataaatca
      421 gtgtttaaga tttatggtga tcgagaagaa aaagattttt taggagtcct tcatataaat
      481 aatatgattc cggtaccaaa agattgtgca aaagtgtggt cacctaacag cccaagtgtt
      541 gatgagaagt ataaaatgtt agtaattaag caggcgaagt acttaaaatc acatgagcct
      601 gaaataaatg aatcatgtaa attactttat aattcaaaac taggaaagct tgatcctagt
      661 tactttaaga aaaatcgaaa agaagtaatt acttatcaaa aaataatgac gaatgtagag
      721 tcgttggaac ctgttagtat agcagaatcc cgaaaaaata ctcttgtaca aactcgaagt
      781 atagatatag agtaagatag gctaaaccta tcttatttta ttatttatat cagacacgca
      841 tttaaacata gtaatatagc ataaaactta aaggatgatt tctatggcga gaccgagaaa
      901 atacaaaact gaagaagaaa gaaaagaagc actaaaaaaa gcaagaaaaa aatgggttga
      961 aaaaaatcag gaaactatag cctatataag agctaaatca tttgctaaga aatttattga
     1021 aatgtccgaa gatgaggata ttgaattact ggaaaaatgg cttgatgagc gaaaaaataa
     1081 tagacaaaat gatgaataat aatagttgac tacaagtagt caaaggtgta gaatgatttt
     1141 agggatagaa atatcccttt tctaaaagtc caatgactac aagtagtcaa aaagaatcgg
     1201 gaataagaat accgattttg gcctaacgaa aggaggatat atgtatttag gggaacgatt
     1261 tattaaataa aattctaaaa tatcaagtaa tataagagta tgatttttaa aatgagtgtt
     1321 gtttaattta aataaatatt ttcctaaccc ctatgtatta tttgagggtt atgtagaaaa
     1381 aatattgaaa ttatttaaaa accgctcata atggaaaata atgaattcta ttaataaatc
     1441 gttcactttt gatatttttt aatagccttt tttgtaggac ttttagagaa tttttttgtt
     1501 atcggtgatt tataaggagg ggctaaattt gatagaaaat agagttctgg gtttcgtgga
     1561 gtgtaaggat aatatagacg atactttaga agtccttgaa agtattatat ttaacactaa
     1621 agttctcgat ttgaaagcag ttaatggtga tatggtaact catatcatat tagatgaaat
     1681 gagcgcaaaa gagattggtg aaactttaat agcatgggct aaaaaagagc tatagagata
     1741 attttaaaaa aagaggccaa ttgactacaa gtagtctgta tagtataata aattatcaaa
     1801 agtgaggtat acatgatgaa atcaaagttt ttgggatatg taattgcaat caatatgttt
     1861 aaagggggag ttgggaagtc aactcttact caactaatgg ctgatattct atcggctagg
     1921 ggctttaaag ttttagttat tgatgttgat ccacagggaa cagtaaccaa aaaatttcaa
     1981 aggcaatatg atttaaaagt agttgggaaa atgacattgt ttgaagcagt aattaatatg
     2041 tctttcttag attgcatttc tcctgtaaaa gaaaatttag atattgtact tggatcagaa
     2101 caaatgtcga ggtttactaa agttttaaga gatatgtgga gaagtactgg ttttactgat
     2161 gaaggcgaag aagttttttg gaatttattc aattatatga tcggtaacga taatttaaga
     2221 gatcaatatg attttatttt gtttgacact attcctacag tttcagaatt tacagataac
     2281 gtttatgtgg ctagtgactt cttagtaatc cctacccaaa cagaacaaga ttcgatagat
     2341 aatttgcata gtaccgttac taattatcaa actgctgtaa aagaagtaaa tggagactta
     2401 gaaatattag gcattgttcc atatctagta gatactaaac taaaaacagg attagaactt
     2461 ctgtcagaac ttcaagaaga gtatggagat ttagtttatt cgaatattgt ttctgaacga
     2521 ggagtaatta aacgttgggg cagagaagga ctgaataaag ctatcccaca ttctaaaatt
     2581 gcttataaca tgtatgtaaa tattgtagaa gaaacgctaa tacgtttaga aaaagctacc
     2641 ggagaaaaga ttttagagca tgtttaggaa aggtgttgca tgatgtcgaa aactaaaaaa
     2701 gtaactagtg ccggcaataa tggttcggga aatccgttcg gaataaaaag cggtagtggt
     2761 ggatttaata tagggcaaca aatgctaaaa ggtaaagctg gcattaagtc cactggtgat
     2821 aaacgaaaat ctgttagagt accggtggac ttatacgacg aagttgttaa ggacttatca
     2881 tatggggcta aatattcatt aatgacagaa attttagctg cttttttaaa agaattcgaa
     2941 atacacgaaa atagtatagt tactgatttt ggtaagttga aaacgaggcc ggtaaaaatt
     3001 tctgaagaag cctttaaagt tttgaaagtt ataaaatttg aaacagatca atcaaatact
     3061 aatattattg cttcagcttt gacatggtat cttgataaaa gaaaaggaaa agcataatga
     3121 aatgaggtaa gactatggat cataaatcaa attattcata caaagaaaat tacgtttgga
     3181 agtatgtagt ggccgtcttt ctattggtag gcggagttat tgcactggtc aaatgggcta
     3241 taattcctaa ttcattagtg tttatcaatt ggtttgttcc aaatttttgg agtttttttg
     3301 gctatatatt gataatctgg tttatgttta aagtactttc ttttttctgg tcgcctatac
     3361 attttatgct tggaaagtgg tcttacctaa ttctaatagc aacagctatt tatatcatta
     3421 tgcagattag ctaattatgc agattttaga agacattaaa atttactgga aaaggataat
     3481 aggctataca ttaattcttc ttggagtaaa tttagtactg tcgatgatag cgttaatctt
     3541 tgttaaacaa ggacaagaaa atgtaactga aatagtatcg tttacttcaa ttttgataag
     3601 tgggattttt gctcctttct ttgaagaaac cttaatgaga ggaattattc aaaatcagtt
     3661 gaaagtgaaa acaaatttgt ctacaagaaa catatatata atcgttgcca ttttgtttag
     3721 tttatttcac ttaaatttat attttgttcc ttatctcatg acgagtttaa ttttgtcttt
     3781 tgtctatgat aattcagaga aaaggctgat tgttccagtt actattcatt gtttttacaa
     3841 tttaattgta atactgttaa gtatagtttt ttacaattga aaaaaatgga caagacccta
     3901 caaatacaag gtttttgtct attttttttt attagttttt atctatcagg ttgatagaaa
     3961 ttatcataaa tgatataatt aagtatgaac tgatttagtt ggaaaaatta aaggagttga
     4021 tagctgttgg caagatcaag aattgacaaa attgaacaga ctaaaaaaga tcaaaaaggt
     4081 tttaaaaata aaacaaaatt ttttaaagct gaagacgtta agaggatagc atggtctgta
     4141 aaaacatttg atgaagatcg ttatgttaaa gtttcaatga atggtctgtt tgtacagctt
     4201 ggtaatttca gtaattttaa aactattgat gaactttatc cggaaataag aaatattgtg
     4261 gatagtaaag gtttaatcgt agctaaacgg aaatcaagaa actctggtct tgcttattgt
     4321 aaagacaaag ggatcatgat tccggcctaa atcagtgaat ttgttccttg aaaacttaat
     4381 actactcgta ctgtagcaat gttgctatgt tacagataaa agcgtaaggc taacaagcaa
     4441 aatagcaacc aagcatactg ttatttcctt tgccaagagg tatacaaata cgttagcaat
     4501 agaaataata aggttagtat ttcacgccaa aaaatacaaa agtcgatcca ttgaaactac
     4561 taattatgag gtggcgtaaa tggtaattat tcaaaatttc tatctaggaa gaacctatga
     4621 aaatcgtcct agagatcaga atgttgacac taaaaaattc agcacagtaa taaatgtgct
     4681 agatacaggg taactcttta ctacaagaaa tgtagtaaag agttgatatg attctttatt
     4741 acatttcttt ctaaaggaag tgtgataatg aaaaaactaa aactgattag cgttgctcta
     4801 atcagcctaa tcactttatc aacagctcgc caatcagttg tttcggctga aggaacgtct
     4861 actgcggaag cggtagtaga agagtctggt gataaaaaag ttgctataga cgacaaagtg
     4921 aaaaacgaag cggagaaagc aactgttatt caaggtgcgg atggccaatt ttatgtagtt
     4981 gcaaatgaat cagacaaaga taaactagtt tcttctgaaa cgccacaaat cgctgagaaa
     5041 acagaaggag actttacagc tgttgaagta gggattgatg aaaaacggca agaaaaagcg
     5101 gatcaggcag atcaagaaat aaaagacttt gatgaaattg atttagatga tgttgaatcg
     5161 ctgcaatcgg cagtcaatag tgaagatctt ccatttattg aagaagatca agttgctgaa
     5221 gaattgtcaa aagccaaaga tttagaggaa tccgaacaga ttttgatgca aacacgtttc
     5281 ttttatgcag aagagatcct caatcaaacg aacaatctga atgacgttcc ttctatgcta
     5341 aaagaaagta aagttgcctt actggcttct gtatcaaaaa cagtatctga aacaacaatc
     5401 aatccgcctt ccggctatcc ttggttaatg tacattgttc gtagcaacgg atattcttat
     5461 acagaagatc gtgggattcg ccggacgatc gatgggaaag atgcgttttg tttacaagca
     5521 ggggttacgg cggctaagac ttatacggac ggcggcagta gttacttgag tacttctcag
     5581 aaagaaatga ttacgtccat aattagtgcc taccgtgaat tgggtggttg gacaaataac
     5641 gatgcaggga aacagcgatg gatggctgca cagattcttg tttgggaaaa ctcgaatgag
     5701 aagccgaaaa gtattacaat cacaccaaat cataaagctg gcgtgactga ttggaaaagc
     5761 aaaattgcaa ctcgtgcaag tcagattcgc acacgtcctc aatgggatgc aacatcaaaa
     5821 acagtttatg aaggtgaaaa agctaccttt actgtgaaaa atagttgggg agatcataat
     5881 ctctacgtag aatccgtcag tggtggcgca aaagcgacta tcagtggaaa taaattgaca
     5941 gttgatacaa atggatcaac tgcgacaaaa atcactgtca acatggtaaa agggtcaaaa
     6001 acaaacgaaa aatatccgac tactgtatgg gtgaacggtt cctatcaaaa actggtcacc
     6061 ggttcttttt atgtttcaac ttctgtaact gtgaatgtga agcgaaaagg gacagttgat
     6121 gtcttgaaag tagatgccga aactaaaaag ccactagcaa atgcaacttt ccgctttacg
     6181 tatgatggca agactaaaga cgtaaaatcc ggaacggatg ggaaggcaaa actaggggag
     6241 cgattaaagg ctggtacagt tgtcaaagtg aaagaaataa ctgctccttc aggctatcaa
     6301 ctggattcga gtgaattttc aattacagtt aaagaaaatc aaaatgttac cactacaaga
     6361 accaataaga agtctacagg ttctgtagaa attgagaaaa taggcgattt ggatgaatta
     6421 ggattgccta atgtagtatt cacgatctac agttccgata ataaaattgt aaaagacaac
     6481 ttaaaaacag atagcaatgg gaaaataaaa gttgatgagc tacaatttgg taaatattat
     6541 gcattagaaa aacaaggtgt tactgggtat gatcccgatg ggaaaaaata caattttgaa
     6601 ataacccaag atacgtcttc cgaaaaaccg gccaaagtga aagtaaccaa tatctatgag
     6661 gtatctgtaa acgaaaaagt atcagatatg aatgaaaatc aggtgttaaa aaatgtttgg
     6721 tcagctgatc caaacgatta tcttcagtat gacattacaa caggaaatct aaaaaaatcc
     6781 ggagaaagaa acgtccaaag tttcactatc gaagggtttt acgataataa aaatgtagat
     6841 gtttcaaaaa gtgaagtata tgttggatca acaaatgtaa caaattcttt tgatattaat
     6901 aacgacattg gcaacggaaa aataatcgct aaggcgaagg caaccgtgtt aacgaataat
     6961 gatttttata ataaatccta taatttacgg atcactatga aaataaaaaa atctactctt
     7021 gaaaacactg aaaagcaaaa accgttaacg attgttaaaa acagtaaaat tactgtcggt
     7081 tccaaaatat ctaagtctgt gaattctaat gatgtagaga cggttttata ttcaagaaaa
     7141 attactatca atcatatcga tgaaaaagat aaacaccttt tgaaacaaga tattgattat
     7201 aaatacgatg gtgaaacata tgaatataaa cctagaatag atttgtttga taatgatgga
     7261 aataattata aatctgctgt agtccataaa gggaaaattg aaggaaaaga tacagtttta
     7321 accactcctt atcatattcc agtgttttct gtgaatgtgg atcagattca aattgatacg
     7381 gctagagcag ttaaaaatgg ctatttacct acagaattaa tactaagcaa aaaagaagaa
     7441 tatgaaaaag agttagaaaa aattgtgttt aaagtgaaac ttacagatat agataacaat
     7501 aaagttgttt atgatgaaaa cttcaaatac aaagaattta atagtcatat gtcgattgat
     7561 ttgcctacag agtatttaac aaagaataaa aagattaatt atagtattga tattctttta
     7621 gttgaaaatc cagataaaaa taagtttgaa actgaaacca aggatttaag aacgatcggt
     7681 tatacttctt ctgagaaagt tttaacgaac aaagatatga caaataacca agtcaactat
     7741 tcagcagtag ttagaacagt gaaagaaaga gcgagtaata aaccggttaa agagttcatt
     7801 gaaagtatca attataaatt tactgataat acgaaaacaa aaacaggata tggtatatca
     7861 gttgatttag aaccaacata tagaaatgaa attgggaagc aaatagatat gaaagtctat
     7921 agttatccga tgaatgaatt gattgacagt aaaattgacg aaatctatca aagtgggaga
     7981 aaagaaacag aagttaagat ggatcgctta aagaatgatg ggaaagttga gtcaggtatt
     8041 aacactcaaa atctttcatt caaatttcca catataaata tcgaaagaaa aactggaaat
     8101 atatatgctg acagtcaaaa aaatgatgaa tctattaagt atgaattgat cgatggcggt
     8161 agaaaattat atgtgcctat ttggtgcgat ttggggaatt attctattgc tatcaaaagt
     8221 agtaactttg gtggtaacta tatctcgttg gatatgaacc aaaacataga tgtttatgcc
     8281 tatatgtatg caacgataga tagtgaaact aaaaaagaag atgaattatt gcttgaacct
     8341 gtttatccag aatccacatt gccagatggt tggaacaaaa atgaaattga ttggctgaat
     8401 aaataagttt gaaaagttat tatctatctg atagaattaa acaatgatat aggatggtga
     8461 tagaatgagg gttcaaagct atggtaacag tgtttttgat gttaaaagag aatttcaaga
     8521 ttcgagaaga aaaaccaaaa agctgatagc ttacttcatt ctatcagcta tcgttttcta
     8581 tattgtaaag tttgttttgg aattaaactc aatggtattc acggatatgg ttcataaaat
     8641 gttaaatgga gaatcaataa attttaactt tgattatatg cttgaaaaag cacagagtaa
     8701 tcaagttgtg gcttttcttg tgagtaccta ttattttatt gaaaaatcta ttttttcttt
     8761 aaaagatttt ttcatggggt tatttaccaa actaacaacg ttttacaata aaattttaaa
     8821 aaattaagga ggaagtttga atgttagatc agaatacaga cagaagttgg tggatgattg
     8881 gttccgtaat cgtaggtgcc attttgattg gaacagctaa agtagctttt cctgaagtat
     8941 ttgatacagt aattacattt ttcaaaaaca tgatccctac aaacttaggt taattccact
     9001 aaaaaattca cgttgttaaa tgcgtagaaa aacaataaag aagaaaaagg gctacataaa
     9061 gtgatttatg tagccctttt tttggatgga agtgatgtaa tgctaagaga tatgcccaaa
     9121 atgatccttc tgatgtttgt tgggttcttt ttaattttag tgattgcgta ctattcatta
     9181 aatggctata cacaagattc ttctgtgaaa ggtatgcaag aaacattaag gactacagcg
     9241 atagcaaata gggatgatag tattcgtgta gaaaaagggc gatttgcttt aattaagtct
     9301 gattttgaaa aagattttaa agaaaaattt gggaaaagta aaaacctaaa taatgaaatt
     9361 actgattaca aatttgatta ccttacagat gggaaggatg gtattaaagc aattaaagtt
     9421 aaggttgaaa gtggatcaaa agaatatcaa gcaacttgta ttttaaatgt tgcaaatgat
     9481 tagaggtgtg aaatttgcgt gttgtagaag aaaagaaaat ttctccaaaa attaaggaat
     9541 cttctaaaag taaaattaat atcgcaagta ttataaaaaa aaatggaatt aaagaaaact
     9601 ctgaagcgat agaagggaac cttgattttt taactaagct tttagaagaa gatagtggaa
     9661 attatgaacc accaattgaa aatgcaaaat tgaatatgat tcgtgatgat ttaacaagaa
     9721 actattccca agaagtttta caagctttaa ccgatgatga aatcagacaa aatctcgtac
     9781 aggttgttct aaaagaacat ggtactttct ttaaaaaaga taaggaagtt gcagaatatg
     9841 tggtacgtga atgtattgga acaggaatca ttgagagaat tatgaccaac gatgaaatta
     9901 cagatgttgg gtggaatggt tctcaactta gtattgaaac caatactaat aaatggttgc
     9961 ttgacggtga taaactaaat attacgcaag aatatattga acgtgttatt tctaaatatg
    10021 ctgttgtcaa tgatcgtgat tttaatagtg gtaatcctgt catggatgga atgtttaaaa
    10081 acgttcgtat atcagctaca cacatagata attcaccaga tggttcaaca ttttctatgc
    10141 gtgtggcaag gcctagatta gcattgaata aagaaaactt tgaagatttt gctcctgatt
    10201 atattttaga gctatttgag aaaatgatga aaacaaaagc aaatatctgt atatcagggg
    10261 agacaggcac aggtaaaacg gaacttcaaa aattattgct aagttttatt gataatcatg
    10321 atcgtatgat tatgatcgag gatgttcaag aaacacatgc taaagaattg tttccagata
    10381 aagatatatt cgcttggatc accactccca aaaaagcaat ttcagattta gtaaaagctt
    10441 ctcttagaaa taatccacga tggatcattg tttcagaaac acgtggttct gaggcgtatg
    10501 aaatgcttca agcggttttg tcaggtcatt atgttgtgac aacacttcat gcgattaatg
    10561 cacgtgcaat acccagacgt tttgtaaata tgtgtgcaag tggttttaaa ataaacgaaa
    10621 atgcagttga ggaagatata aatcgttatt tcgattttgg cgttcatatc aaaaaaacag
    10681 tagttgatgg taaagtgatt cgttatttga atgaaattgt tgaattttct gctgacgaac
    10741 cgactacagt ttttaagcaa aaatttatca acggaaagtt tgtcactgaa aatggtagtc
    10801 tttccgaaga atttaaagaa aaaatggctg aaaaatttat tacgtttgat tttcctgaag
    10861 agcaaaccaa tgcagaagaa tcgcttcctg aagaagaggt gatttaagtt gaaagtacag
    10921 gctatgacaa agcgaaaagg cctcatggat gtttcggagc taaacgctta tcaagttgcg
    10981 tatggaaaac cgctagatta taaggattat ttaaactatg ttggattacc ttgtttgttt
    11041 atggggattt tttctttcgc tttatgttac tactggtgga ttgctttgat ttgtgcggta
    11101 atcggaggcg tatatggttt taaagttatc atgcctaaaa gtattaaacg ctcatatgaa
    11161 ttaaagagtt tacaggaaag aaataaattt gttaacaata tgactcaaat tttgactgat
    11221 gacagtaaaa cagttactaa agcgttagct atagcaaaac agcgaacaaa aggtgaatta
    11281 agagaagata ttcaaatctt agaatcacgt ttgcaaggcg ctgataaatt tcagattgct
    11341 gaagcttttc gtgaaatcaa caataaatat tatagtgatg ttgtttttac acaatacttt
    11401 gagcaattag aaacagctat ttatgaaggt agaaataatt cagatactat gaagcaaata
    11461 aaaagttatc acaatgatgt gaaacgtaaa acagaaggtt ttatgagaat caaagacggt
    11521 tatcttcaag atatgaagca aatgatgttt attgtcgcta tctttattgg agctataact
    11581 gttgcttttg ggttttcaac tttctatgaa ggctttgctc ggtcaccaat aggatgggta
    11641 tttggttcta tttactattt gattatggtt agcttcgtga agtcattttt taaacattat
    11701 tttgatgatg aaataatgag tttgggggta agcaattaat gaaaattaag gctttaaaaa
    11761 ggaaaagaaa atcaagcgtt agccccttga aaattacgaa aacagaacat aagagctttt
    11821 ggattaaatt gctaggtgat gtgtattatc aaaaattacg tgaaatgaac aattccaatg
    11881 ttgctattat taaatggcaa aagcaacggt taaaaaagtc attgattttt atcgtggtcg
    11941 gatttttaat gtatctattt ttagggaaag aatggtacta cttagccgga gggattgcta
    12001 tttcagtagg tttttatgtt tggcaagcaa aatcaattaa tggtatctat aatcaatttc
    12061 ggtttgaacg acacctacaa tttagcaaat ttacacggct attgatccca tatctgaaac
    12121 aaaaaagaga tgggggaaat ttatatggtg tttttgcaaa aatcgtaaaa agactagatt
    12181 atgataccga taaaaagcta ttaatgaagc ttatgcaaga aatgacagat cagcctaacg
    12241 atattagacc ttttacggaa tatgcagaac agacttctgg aacagatatg tctatattat
    12301 ttatgtctac agtttatgat attcgacaag gttcggcaga tttagatgtg atcgaagagc
    12361 tagatcgatt agcttctgaa gaattaatgg ccggaatcga ttcaatcatt gactataagt
    12421 cgaggcgttt tgtcttcttt cctacaaaaa ttgcaatgac ttcatttatt ttagttgtcg
    12481 gttttgcagt aggagttctt ttcatgaacc taaaagatat taactttggg caataaagga
    12541 aaggagaaag tcaatgcaag aacaatccgt tatggaaatc attaaatggt ttatagggtt
    12601 aattatgatt atgacaatgg tttctatagg actcttttgt gttcgattac aagatattaa
    12661 tacttttaag caacaagtaa attatcaaat cgaacgaaaa ggaggtctaa ctacagaagc
    12721 tgttgaagct atcgacaagt attcggctac taattatgat gggcgtttta caattgaaag
    12781 cgataagcta aatcagaagg tatcttacgg agaagaaatt gattatacga tcaaagcaac
    12841 tattccaatt caaattttac cgcttcctga tgtatcttta tcgtttaaag gaaatagtgt
    12901 ttctcaaata agatgagagg aaggatagaa tgcaagagaa taatgtttcc agaatagttt
    12961 atttgattgg cggattgata attttatcct tagctattgc cggtcttgta gagcattggc
    13021 cggaaataaa gttgttaata aaaaattttt atgacaataa aatgattaaa atgtaggagg
    13081 tatagatttg gaaacaattc aagaaaatcg taaggataat caagaaataa ttgaaaagaa
    13141 caaaaaaaca agaaaaaaga aagtagataa atttacagaa atgaccgata aaatcattga
    13201 aaagaaagga cttaaaccta aagattttga ccgtcaagca attaaagaag cacgacaaaa
    13261 attgtatgac gattttgtta aagaaaacat atcacagctt ttagattaac aattaggggg
    13321 aaataaaatg aagaatttag ttatcagcaa gttttcaatg ttaccaagaa tggatttttt
    13381 cgacaatgta agtaagttta tgtcaaatat gacttcaaaa ctacaaggaa tttcgttatc
    13441 tgtaattatc ttttgtattg tggttactgg tttgatgttt cttttcggag aagggccaag
    13501 tcgtactgct aaaaaatggt taacgtatat cgtgattgga gctgtaatta tctttggagc
    13561 ttcaactatc ggacaaacga tcaagtccgt tggtggtttc taaggagaag cttcttagaa
    13621 ctaaaaaatt cgcaagtatt gcatgaaaga gcattgagtg attaatgctc ttttttattt
    13681 tgaaaaacta taaactaagg agatttaaac tatgaaccaa cgattcgaat ataattttga
    13741 agatgtagaa cctaagttgt tgaaaaaagg aaaaggatta aaaaagaaaa acaaatatac
    13801 tctcaaacaa gcagaagcac tagtaggatg gattcaacac aattggtcag acaagacaac
    13861 cattaccttg atctgttcag acacagaaga aggaacaaag tatgcttttg atggaattga
    13921 aattacgcca ttttcgatag gcggacaaga aaaacttctg ttttctgata ttgaaggaca
    13981 aattttacaa gcacctgaca gcatgatttc cgatcctaat aaactgattt tggatattaa
    14041 cgatcaatac aaacatgaac cagatgtagc agaaccaaca aaagaatcaa agtcaaaatt
    14101 aaatctgaag cttccttcag tagtattacc attcgctaag aagaaaaaca aacttatgaa
    14161 cgaattgatt gaaccagaag aaacagatat ttatgaagaa gaattagaag aacaagaaga
    14221 gattgaagaa ttagttgttt cttctgaaga aacagaagaa gtagaagaaa ttgaaactgt
    14281 ttctgaaaat aaagaagaaa tagaagaaat ctttgacttt gaagatgaag aagaagtgga
    14341 aaatgtaaag aaagacggat cgactacaag tagtcaaaat ggattggtag ctgtaaatga
    14401 tccgcaagtt ccaccgtatg tgccttctca agctcctaat tacactccac agtatcaagc
    14461 gcctaatgtc ttacaaccaa taagaaaaca tgaaacagtg cagttagtta cccttaaaga
    14521 gtactgtgat ttgggagaag aaatcgaaac ttcaatttct caaatggtcg aaaagttgaa
    14581 acccgaatca ttattcaaat tcatagggat tccttctaca ggtcttacaa atactcgtat
    14641 tgatgaatat agaagtaatc atgctatgtt gagattagct gaagtaaaat ttgaaaatct
    14701 aagagagcat tattcacggc aaattatttc ttttaaagca gaagcattag atcgtttacg
    14761 aactgcggta gatattgctt ggaagaagcc ttacgattta gatgtgaaga aagacaaggc
    14821 agaagagtta tcaaaaatgg aagtagaagc agaagataaa ttaaatgagt tcgtaaaacg
    14881 tcaggaaaac aatgtaaatg ataaaatcaa aaagtttgag atcgaacaag aaatagattt
    14941 aaaaaacttt attgcgagac aagaggcaga taaagcggta tttattgcta cagagaaaga
    15001 tcggactcaa aatttgattg attctaaagc agaatcaatc aatgcagatt tacttgaagc
    15061 aaaagataaa tttatagatg aagaaatgta caaaatgaaa attgaaatga acaaaaattt
    15121 atttgatggt aagcaaaaaa ttaaatcaga attggctact gaactcgcta aggcaaatga
    15181 caatgtttgg gagaatgcac tgaaatttat acaagaaatt caagaagaaa ttgaaactaa
    15241 aacaccaatt tgggcagctg aaatcaagga gtttaatagt ttagaagaac aagaacataa
    15301 acgtatgaag caaactaaag aacttgaatt aaaagaggaa agtattgaaa tcaagcgttt
    15361 agaagctgaa atgaatttga agcgtatgga agaattagat catgaaaata aaatgcttaa
    15421 aattaaactt gaaacggctc tttcaaaaaa tgagttacta gctatggaac aagatacaaa
    15481 gcaagtggtg aatggatcaa acagagttgg cgcacttaat ttatttagta aacgttaaag
    15541 gagaacggat atgagtgttt ttaataataa agttatgtta gggaactgtt atgtgaacca
    15601 cgttggattt tatgatgggg gaacatacag agttttattc ttacgtttat ctgtttttag
    15661 ctactcaaaa gatggaaagg gttattatga aaatgttaat gcccaaattt cttgtcctgt
    15721 agatagaaat gggaaacctt cgactacaag tagtcaccct aaagctttga tgaataaatt
    15781 tatctatgaa aagttggatc gcaatttggt tgagggaatg aagataagta ttcatggaaa
    15841 tattcgtgga acagaacaag tacttcttaa cggtgggtgg aaaaattttg gtcaattatc
    15901 gtctttagaa aaagaagaat atcaaaaaat tcctaaaaat gaactagtaa caagaaatca
    15961 aacatatatc tatatagaag aggtaagtat gccagaaaaa gaagaattta gaaagaccca
    16021 atctaaggca acaaaagaag atgtggaagt tgtggaaccg aatcaagcag atgaaatttt
    16081 atcagatgat gctgatccgg tgaatgatga aggggaagtt attgaagttt ttgaagctga
    16141 aattatcgaa gaggaagaag ttaaaagtgt tgataaaaga atgtatcgag agaagtcaaa
    16201 aggttatgtg aattttgaag atatgtatta tgacgaatat tctaatgaac caccgatcaa
    16261 ttagtggggg tcaagatgaa gctagtacaa aatagtgaag tgaaaaaaag aaaaaatgga
    16321 tttgctaaaa actcgaataa aattttggta aaactgaaaa aagcaggtag tttaaaaaaa
    16381 aataaatatc atactggaaa aattgaggga gaagatttac cgaaaagtct acaagataaa
    16441 ttgaatgttc ccctttcatt tctaggtata tttctaggtt caatagtttt tctagcaata
    16501 acgtacttat tgaacttttt gaagtctttg ttagttgcaa tcggccagtc agaaagtggt
    16561 ctacttggag ttgagttcga tccaaaattt agtttgacac acctaagacc acttggggat
    16621 acgaaacaat actttatgtg tgctgtagtt gtggtaatag gagtcgctta tttaatatat
    16681 gggaaattaa atcatgaaag cgagaaaaat cttgtttacg gtcaaaaagg agattcaaga
    16741 ctttcaacta tcaaagaaat taaaagagat taccatgaga taccagaaaa gacagaagtt
    16801 tatgatggta ttggtggtgt gcctattagt cactatcaag ataaatatta cattgataga
    16861 gatacagtaa acaccgttgt tttgggagct tcaaggtctg gtaaggggga aacatttgtt
    16921 gttcctttat tggataacct tagtcgagca aagatacaaa gtaatatggt tgttaatgat
    16981 ccgaaaggag aattgtttag cgcttcaaaa gaaacacttg aaagacgagg ctatcgtgta
    17041 ttagtgctaa atattgatga tccacttgaa tcaatgagtt tcaatccttt acaactagta
    17101 attgatagtt gggctaacaa ggactatcac gaagcgagta agcgagcgaa tactttaact
    17161 tcaatgctct ttgcttcagg aatgggtaca gataatgagt ttttctacaa atcagctaaa
    17221 tcagcggtca atgcgattat tttaacaatt gtagaacact gttttaacaa tgattgcatt
    17281 gaaaaaatta caatgtacaa tgtggctcaa atgctgaatg aattagggag cttattttac
    17341 actgatccca agactggaaa agaaaaaaat gctttagatg aatatttcaa tactttacca
    17401 caagggaatc aggctaagat tcagtatggt tcgacttcat ttgctggtga taaagccaaa
    17461 ggatcgattt tatctactgc ttcacaaggt atcgaaatgt ttacaagtga tctgttcgga
    17521 aagttaacaa gtaaaatgtc tattgatttg aaagagatag gttttcctaa gtccatacaa
    17581 tttaaagtca atacgaattt agttggaaag aggattagta tttcattttt gagaagagaa
    17641 aatgatgtaa ttagactaat taagaagtat cgagtgaaag taaaagcttt agggctatgt
    17701 gttttaaatt ttaatgaata tcttcaggat ggagatatgt tggatatacg atatgaagaa
    17761 gagaaatcga gagcatggta tcgtattgaa tttccaaaaa aacaagcaaa tagtcatgat
    17821 acttctttag tcactaaaaa attcaagtca ggtgcaaatt tttctgactt agaaatcagc
    17881 actttacgat taaaatatac ggatcaacca actgctgtgt tcatggttat tccagattat
    17941 gatccgtcga atcacgtact tgcgagtatt ttcatcagtc aactttatac agagttagca
    18001 agtaattgta agcaaacacc ggataaaaaa tgttttaggc gcgttcattt ct
//
LOCUS       DACDSY010000054        17747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDSY010000054 DACDSY010000000
VERSION     DACDSY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.054"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            113..1798
                     /locus_tag="IWK44_001457"
     CDS             113..1798
                     /locus_tag="IWK44_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP9549993.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            1812..3095
                     /locus_tag="IWK44_001458"
     CDS             1812..3095
                     /locus_tag="IWK44_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9549994.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            3165..4034
                     /locus_tag="IWK44_001459"
     CDS             3165..4034
                     /locus_tag="IWK44_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9549995.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            4031..4867
                     /locus_tag="IWK44_001460"
     CDS             4031..4867
                     /locus_tag="IWK44_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9549996.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            4893..5960
                     /locus_tag="IWK44_001461"
     CDS             4893..5960
                     /locus_tag="IWK44_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP9549997.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            5957..6784
                     /locus_tag="IWK44_001462"
     CDS             5957..6784
                     /locus_tag="IWK44_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9549998.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            6829..7854
                     /locus_tag="IWK44_001463"
     CDS             6829..7854
                     /locus_tag="IWK44_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9549999.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            7851..10034
                     /locus_tag="IWK44_001464"
     CDS             7851..10034
                     /locus_tag="IWK44_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9550000.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            10034..11164
                     /locus_tag="IWK44_001465"
     CDS             10034..11164
                     /locus_tag="IWK44_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9550001.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            11161..11838
                     /gene="pgmB"
                     /locus_tag="IWK44_001466"
     CDS             11161..11838
                     /gene="pgmB"
                     /locus_tag="IWK44_001466"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9550002.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            11922..12449
                     /gene="msrA"
                     /locus_tag="IWK44_001467"
     CDS             11922..12449
                     /gene="msrA"
                     /locus_tag="IWK44_001467"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9550003.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12650..13336)
                     /locus_tag="IWK44_001468"
     CDS             complement(12650..13336)
                     /locus_tag="IWK44_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP9550004.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            13416..14954
                     /locus_tag="IWK44_001469"
     CDS             13416..14954
                     /locus_tag="IWK44_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP9550005.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            15074..15541
                     /locus_tag="IWK44_001470"
     CDS             15074..15541
                     /locus_tag="IWK44_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP9550006.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            16060..16503
                     /gene="rplM"
                     /locus_tag="IWK44_001471"
     CDS             16060..16503
                     /gene="rplM"
                     /locus_tag="IWK44_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP9550007.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            16519..16911
                     /gene="rpsI"
                     /locus_tag="IWK44_001472"
     CDS             16519..16911
                     /gene="rpsI"
                     /locus_tag="IWK44_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9550008.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(16999..>17747)
                     /locus_tag="IWK44_001473"
     CDS             complement(16999..>17747)
                     /locus_tag="IWK44_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9550009.1"
                     /translation="ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTN
                     WIEAVNDDFNNHLLTFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDK
                     YQRKKFTKGRKDTVIQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGE
                     VNCPVHADYLNYRINSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSD
                     ISTTKIYVNTPDIVDKAVFEAFQRGLNKCD"
BASE COUNT         5771 a         3247 c         3808 g         4921 t
ORIGIN      
        1 tttgtatttt tgtgtatatc aaaattattt aaaaaaatgc tattgacagt aagcgttttc
       61 cataatatga tcaaatcgtt gggaacgctc ccaaaaaaga aaggggaatc cgatgtatac
      121 aaaactttta caagcgattt atcgcgactc agatttgtct gagaaaaccg cctctatgat
      181 tgaacaaaaa gtcagtgttt atcagcagaa gaaaggccag gcaggcaaac tgcattggga
      241 cgaagaagat attttcttga ttacttatgg ggatcaattt tttgaagagc agcaaccaac
      301 tttgcgtaca tttaagaaat tttatcaaaa gtatttggca gatacattta gagttgtaca
      361 tttcttgcca tttttcccat atacatctga tgatggtttc tccgttgtaa attatcaggc
      421 aatcaacgac cgtttgggca catgggaaga tatagaagaa atgagaagcg aaacacggtt
      481 aatgttcgac tttgtctgca atcatatgtc tgccgaaagt acttggtttc aggaatattt
      541 aaacttgaat gatacgtaca aagatttttt tattgatttg ccactggata cagatacaag
      601 cagcgtgact agaccaagga ccagcccgtt acttacaacg ttcattgaca aaaacgggaa
      661 agaaaaacac gtatggacga cttttagcga agatcaagta gacttgaatt ttaaaaatcc
      721 agaagtttta ttgaaaatga tcgatattct tttgttttac ttagaaaaag gcgcttcatt
      781 tattcgcttg gacgctgtgg gatttttatg gaaagaacta ggaacttctt gtatacacct
      841 gccagaaacg cataaaatca tccaattatt ccgtgcaatc gctgatgaag cagctccagg
      901 aacggtattg atcactgaga caaatgtgcc acacaaagat aatatcagtt attttgggaa
      961 tggcgagaat gaagcacaga tggtctatca gttttcgcta ccacctttga cccttcaagc
     1021 gattcgttcg ggtaatacaa gtaagttgaa agaatggtta aaaaccatcg atttcgcatc
     1081 gaaaaaaaca acttttttta attttttagc ctcccatgac ggtatcggat tgaatccggt
     1141 acgaggtatt ctgccggaaa aaacgatcct tgaattagtg gaacagctga agaaagaggg
     1201 tgcccgagta aactacaaga aaaacccaga tggaacgacc agtccatatg agataaacgc
     1261 tacgtatcta gatgcgttaa gttctccaac agatacgacg gaatggagaa gcaaacgctt
     1321 tttagctgct cattcatttt tggtaacgtt accaggcgtg ccggcaattt atattcaaag
     1381 tgtgctggga agtcgaaatg attacaaagg cgtagaaaaa acaggagaga atcgaaaaat
     1441 caatcggcaa aaatttttgt tagcagatat tgagaaagag ttgcagacag atccattaag
     1501 aacagcagta tatggtgaat tgacccagat gctatctatt cgaaaaaaag aaaaagcctt
     1561 ccatccgaat agtttgattt acttactgga ctcagcagat tcttttatca gttttattcg
     1621 cgaagcgaag gaagagaaag tcttagttat ccataatttt tccaatcaat cagcaggtta
     1681 tcttttacca aaaggagatt acctagacct gttttctgga caaattatcc attgcagtca
     1741 agaacgaaag ttgatattcc agccgtatga atatatgtgg ctgaaaataa taaaataaga
     1801 ggtggaaaaa aatgaaaaaa attattgtta ctacctgtct gctagctact ggatttcttc
     1861 ttactgcttg tggtgaaagt caaagctcta acgaatcaca acagacaacg attcaattca
     1921 tgcactcttc tgtcgaacag gaacgattaa gtgtgatcaa tgaactggtt gctgattttg
     1981 aaaaagaaaa tccagacatc aaaatcaagc aagtgccggt agaagaatca gcgttcaata
     2041 caaaaatcgt tacgttagct cgttccggta aattaccaga agtaatggaa gtcagtcagg
     2101 attttgctaa agtgatggat aaagatgaac tgattgatca gacggcagtc caaaacgtta
     2161 tcgaggaagc tggagaagat aattattatg acggagccaa aaaccttgtt cgttcagaag
     2221 atggaaaaag ctatattgct gcaccaatta gcggctgggt tcaaggtatt tggtatgaca
     2281 aagaaaaatt atcagaagct ggtttttcag aaccagaaaa ttgggatgat attttaaaaa
     2341 ttgctcaaca tttcacagat aaagagaata aacaatatgg aattgctatg cctactgctg
     2401 aaggaacaat gagtgagcaa gcgttctctc aatttgcttt gtcaaatgga gcaaatgttt
     2461 tggatgatga aggcgaggta acgatcgata cggaaaagat gagagaagca ttgtcgtttt
     2521 atcaaaactt atcccaatac acgatgccag gctctaatga tgtgacagaa atcaaagatg
     2581 ctttcatgaa tggaacagca ccaatggcaa tctattcaac ttacattttg ccttctgttt
     2641 atgaagcggg gaactcagaa aatatcggtt ttgctatccc aacacaaaaa gatcaagctg
     2701 tctatggcac agtatctgga ttgacgattt cttctggtct agacgagaaa caaaaagagg
     2761 ctgctgaagc attcacagct ttcttatctc aaccgaaaaa catggaaaag tgggtcttga
     2821 tgtctcctgg tggtgctcaa ccagtgaata aacaagtagt cgaactggat ggttacaaag
     2881 aaaatgaagt catcaagtct tttggcgagc tgccatctga gattgctagt gcttttgatg
     2941 aagtacaagt ctttggacta gtcggggaaa agaatttcac gaaaatggga gatatcacaa
     3001 gctcaggtgt gatcggaaaa gcagtgaacc aagtaactgt cggaaacgaa gatgtcgatc
     3061 aagctttagc tgacgcacaa aaaaatacga aataacaaga agttctgcca atggtgttcg
     3121 aaaaaagtag atagctattg gcagattatc agggggaggt ttcaatgaat cgtccaagga
     3181 aacattctga cttaaaatta gccatgggac ttttagcacc aagcatgctt ttattagctg
     3241 ttttagtgct gattccaatg ctttccaata ttcagatcag ttttttcgat atgccgctga
     3301 atcctaactc agacgccgtt tttatcggat taagcaatta tcttgaaatt ttaagtgata
     3361 gtgaatttta tcgttcttta ggtttaacag ttgcttacac attcttagtt gtagcaggca
     3421 gtacagcttt aggattagcc gtagctattt tgcttaaccg caagttcaga tttaggaaaa
     3481 cggtgcgttc actgatttta ttaagttatg ttacgccatc tatttctttg atttttgctt
     3541 ggaaatatat gttcaataat agttatggaa tcgtcaatga tctgttagta gataggttgc
     3601 agcttttcaa cgaagcaccg ctgtggttcg accagccagt tgctagcttc tttatggtcg
     3661 ttttgtttgc tatctggaga tactatccgt atgcgtttat ttcatttttg gctattttgc
     3721 aaacagtcga caaaacgctt tatgaagcag ctgaaatgga cggtgcaaat gcttggcaga
     3781 agttcaagat cgtgacactg ccggcaatca aaccagtact agcaactgtg attgttttac
     3841 gatctatctg gatgttctat atgtttacag atgtctatct actgacgaat aaagtcaata
     3901 tcttaggcgt ttatttgtat cagacagcct ttgcgttcaa tgatcttgga aaagcagcag
     3961 ctatatcgat tctattgttc atcattattg cagcggtcgt cttgctctca cgaaagaagg
     4021 tggacttgaa atgaaaaata aaaagagcag gaaaatctta ttttacgcga cgattttgtt
     4081 gctattagct gtcaatttgt ttccattttt gatcatgctg atgacatcat tcaaggattc
     4141 acaagaagca attgcaacga cacaaactat ttttccgaaa gaatggactt ttgaacatta
     4201 tcaggatatt ttcaatgctg agatttttcc attcatcatg tatttcaaaa atagtttagt
     4261 gatctccttg atagcctccg taactgcagt agtgattggg atcagcgggg catacgcttt
     4321 gtcaaaacta cgcttcaaag ctcgctcgaa aatcaatgct agcttttaca ccgtttatat
     4381 gttttccggt attttgatcg ttgttccatt atttaaaatc atttccggtc taggtttgta
     4441 tgataccaat acagcactga ttatcacgat ggtcgttcaa acacttccta ctgcgatatt
     4501 catgctaaaa agttattttg acacgattcc gactgatatt gaagaagcag caatgatcga
     4561 tggtcttagc cggatccaga tcattattcg gatcattata ccattggcta tttctggaat
     4621 catttctgtc ttcgtttatt gctttatggt ttcgtggaat gattatttat ttgcttcgat
     4681 tttcctgtcg acctccgaaa aatttacttt accaatcggt ttgaacacat tattcagtac
     4741 accagactac atttggggac gaatgatggc cgcttcattg gtgaccgctt taccagtcat
     4801 cattatgtat gcgttatctg aacgatttat taaaggtgat ttagtcgaag gcggcgtaaa
     4861 aggataaaaa gaaaagaaag aggaaagaaa agatgaaaaa attagttgca ttagaaccaa
     4921 gaatagccgg attcaaagat tacgaagacc gtgagatcga aaagaacgaa gtacgtatcc
     4981 gagtaatttt cgctactcca aaaccaggaa cagaaattgt cgactttaga ggattaagtc
     5041 catttatcga tgaagaattt tcagaagaat ggcgcctatt cttgccaaga gaagaaggaa
     5101 gccaacgcgg tgtggtattt ggcgaatttc aattaggaaa tatgattgtc ggtacgatcg
     5161 aagaaacggg cagtttggtg acagagtatc aaaaaggaga gttagtctgc acttatggac
     5221 ccattcgtga aacgctgatt acaaacggtg ttgataatta taaactacgt aaacttgatc
     5281 ggttggatca atggaaaaat gcagtatgtt acgatcctgc tcaattcgct ttaggtggta
     5341 tccgggaagc acatgttcga gcaggtgatg ctgtagcaat cttcggttta ggcgctatcg
     5401 ggcaaattgc gattcaatta gctaaaaaag caggtgctac taccatcgtt gggatcgatc
     5461 ccatagcgca gcgtagagaa ttagcagaaa aacacggtgc cgattacacg ctagatccta
     5521 tgaaagcaga tgtcggtttt cgtctaaagg aattgacaga taaacgtgga gtagattcag
     5581 ttattgaaac gagtggcagt gctgccgcat tacaagcttc tttaataggg atcggctatg
     5641 gcgggatcat ttcttatgtt gcctttgcta aaccttttcc agctgggttt aatcttggtc
     5701 gcgaagcaca tttcaataat gcaaaaatcg tctttgctcg agcatctagt gaaccaaacc
     5761 cagattaccc aagatggaac cgtaaacgaa tcgaggacac atgttgggaa ttattgatga
     5821 acggttactt ggattgtgaa gaaatcattg atccagttgt gccatttgca gaaagcgcag
     5881 aagcttacat gcgttatgta gaccagcacc cagaagaaag tatcaaaatg ggcgttcaag
     5941 tccgaggagg agaacaatga aattcggtac gcaagatcaa gcatttttcc caagagacat
     6001 tttagaaaaa tttagattta tcaaagactg tggatttgaa gaatacgaaa tcgacggtaa
     6061 gttgttaata gaaaatatcg acacagtcaa acaggcaatc aaagaaacag ggatcccagt
     6121 agtgacagct tgtaacggct acgatgggtg gatcggtgac tttatcgaag aacggcgtct
     6181 taatggtgta cgtcaaattg ctgagatttt gcgtgcacta agcgaggttg gaggcaaagg
     6241 aatcgttatc ccagcagctt ggggaatgtt cacttatcga ttaccgccga tggtttcacc
     6301 ccgaagcaaa gaaggagact ataaagcagt cagcgaatca cttcgttatt tagataaagt
     6361 agccgaagaa acaggtacgg ttatttattt ggagcctttg aaccgatacc aagaccatat
     6421 gatcaatttg ttagcggatg cgcgtcatta cattgactct aatcaattga aacatgtgca
     6481 gatcatcgct gatttttatc atatgaatat tgaagaagat gatatttcaa aagcattgac
     6541 tacgcaaaaa gagtatgtgg gtcatatcca tttagcagat aatcatcgct accaaccagg
     6601 atctggcgca attgatttta ctcgccattt tcaaacgcta cttcaaaatg agtatgaagg
     6661 ttatatggta tatgaatgtc gtttgcgagg agaaaatgaa gtgaaaattt atcaggaatc
     6721 attgcaatac ttgaaaaact gtctggaaac agcgcgtcat ttaaatgatt tagagaaaga
     6781 atagagaaag aataacaata agtaaataag caaaggagag agaaataaat gaagaaagta
     6841 gcaatcatag gcgctggaca agtagcagaa aaagtacatg ctgcttatta tcaaaaaaga
     6901 tcagatgtct tagaattggc cgctgtggtt gatccagtca gccagcgagc agaagacttt
     6961 tgccgacgta caggattttc tcatgcgtat acatcagtta cacaaatgct gaatgagatt
     7021 tctcccgata tcgtgagtgt ctgtacaccc aatcgctttc acttcgaaca tgtattggct
     7081 gccctcgaag ctaatgcttc agtgatgtgc gagaaaccac cagctatgac agctgatcaa
     7141 gcaagaaaga tggcagagac agccgataaa aacgggcaga ttttggctta tggattccag
     7201 catcgttttt ctgacgaagc aacaatgatc aagaatcatc ttgatgttct cggggatatt
     7261 tattacgtag aagcgaatgc gcttcgtcgg tctggtgttc caggctgggg aacgtttatc
     7321 gacaaagaac ttcaaggagg cgggcctttg atcgattatg gcattcacat gcttgataca
     7381 gcgatgtata ttactggatt tcctaaagta aaaagcgttc gggcgtacag cttccaaaaa
     7441 attggtacga aaaaaaattc agggaaattc gggaaatggg atccaagcaa attcacagta
     7501 gaagatgcaa tttttggcac actggaatta gaaaacggca agatcatcca actgaatact
     7561 tcgtttgctt tgaatatcag agaagacaaa ctgaatgtgg ctttttgtgg cgatcaggca
     7621 ggtgctaatc tgtatccagg cgaactattc gaagatgtag atggaaagct ggtttcctta
     7681 caaaagacca cgccagttaa tgacccacat gaactaagta tggctcattt tgtgaatcgg
     7741 gcgctcggtg accagactca gatgattgca gatggctggc aaggatatgt catccagaat
     7801 ctggtggaaa atttatatcg ttcggcagaa acaggagaag ttattacctt atgaaagtag
     7861 aagaaaaaca gtttgattta gagtatatca ataagtatgc gagtcagatg accattggaa
     7921 atggattttt tggtatcaga ggaagccagg aggaagacta tcggaatcaa gtaaggggaa
     7981 tgtttgcagc tggtgtatat aaccgacctt taggagcaga atcagccgag ttagtgaatc
     8041 tcccagatgt catcagatgg gaaataaaaa tcgacggtga acatttttct cttgagacga
     8101 tgaaagttct tgcatacgag cgggcactag atttagaaat gggagagctt aaacgctatg
     8161 cgctcattga aactgctcaa ggaaaaaaag tggaagtcat tacttgtcgt gttgctgatc
     8221 aaaataatct ccatcaggca gccctgaaag tggtcatcaa gccgttgacc gaagactgcc
     8281 aagtagatgt gaaaacgggt atcgatggac agcaaaccaa ttttggtaca cagcagttgg
     8341 ttgaaaaaga actgcgagta tttggtgacg atttgatcag tgcatcctac cagacaacag
     8401 aaagtaaaat tgatattggg attgcagcta aattcaataa agcaggtgta ttccaagcaa
     8461 aaaatcgccg gatcaccgct tccttttcag aaaagatcag aaaaaatacg tcctttacgc
     8521 tggagaagat catcgtgctc tctacctctc ttcagcaaac agacccgttt aagaaaagta
     8581 ttgcccaagc ccagaaaagc gttagttata cagaaatttt agagcagtcg atgtcttatt
     8641 ggacagattt ttggcatcag catcggataa cgatccaagg cgattcttct ttcgatcagt
     8701 tagcgatgga cttcgctgct taccatttac agattatgac tccccgcaat gatccaagtg
     8761 ttagtgtagg ggcgaaggga ctaactggcg aaggttataa agggcatgtt ttctgggata
     8821 cggaaatttt tatcctgcct ttctttttat atcagtttcc tgaagtagca aagaatttgc
     8881 tgctgtaccg ttatgatcga ttagaaggcg cacggcaaaa agcaagagat tatcactatc
     8941 aaggtgcact tttcccatgg gaaagcgcac acactggtca ggaagagaca cccaaatttg
     9001 ctgcattgaa tatcaaaacc ggaacaagac aagaagttgc ttcagctgcc gcagaacatc
     9061 atatcagtgc agaccttgct tatgccgttc aagagtatgt ccaagcaaca gaggatcaat
     9121 cgtttttaga aacaaaggga aaaacgctca ttcatgaaac agcagctttt tggctgagca
     9181 gagctgtaaa agtcgatgat catttagaga tacacagagt cattggtcca gatgaataca
     9241 cagaatatat cgacaataat gcatatacga actatctggc gcattataat gtggccttag
     9301 ctgttaaata tcaagctggc gatccgcttt ttttgaaaga atgtcaaaat tttttagata
     9361 agttatattt gccagtgcct aacaaagaac agctgattcc gcaagatgac acgtatctca
     9421 gtaagccagt tattgatttg accacttaca aagaacgtcc tggcagccag tctattttac
     9481 ttgattatgc ccgtagcgaa gtaaatgaac tccaagtcag caagcaagca gatcttgtca
     9541 tgctgttata cttattacct gagtattttt ctcaaccaat cgtttctaag aatttgcatt
     9601 actatgaaaa caaaacaatc cacgactcat cgttgagtaa agcgattcac gccatagagg
     9661 cttgccgtat ccaagaaatg gaatgggcgt atcatttatt ccaagaagcg tgcctgattg
     9721 atttaggacc agatccgcat tcatcagatg acggaattca tgctgcttca ttaggagcac
     9781 tatggctagc agttatattt ggtttttcag gtattacagt aacgaatgga actctttcta
     9841 tttctccaag acttccttca gcatgggaaa aaatttcttt tccttttatt tggaaaaatc
     9901 acaggttgct atttgttttg acaaaaaaac aaatagtaat aaaaaaagag acgaaagaaa
     9961 cattacctat ttttgtcaat ggagaccaat accagctgga aaaagaactg atcttggaac
    10021 ggaggggaaa ataatgcgta ttctgattgc gccagattct ttcaaagaaa gtctgacagc
    10081 cttagaggca gcacaagcca ttcaatcagg ttttcaagaa gtttttgttc aggctgaata
    10141 tgatctgcta cctataggcg atggaggaga aggaacggtt tcatcattag cagacagttt
    10201 aaggttgaag cggcaaacag cagtcgttac taatgcattt ggcaaaaaca aagaaatcca
    10261 gtacgcttca gacggtcaaa ctgcgatttt tgaaatggca gaaatcgtcg gattgaaaga
    10321 tgtcccacta aaccaacaac agccacttag gttatccacg cgaggagtag gagaactgat
    10381 acgacaattg ttgcaacaag gtgttcgaca catcatcatc ggcgttggcg gaagtagtac
    10441 caacgatggc ggcatcggca tggcagaagg tctaggttat gctttttacg atcaagataa
    10501 tcaaaaagta gaagcactgg gagaaaatct aggtaaaatt gctacagttg ataaaacgaa
    10561 cgtgcctatt gagctgatta aagccaaaat cactgtcatt actgatgtta ctaatctttt
    10621 atgcggaaaa gaaggcgctt cttttgtttt cggaccacaa aaaggcttga aagaaaatga
    10681 attagcagtg gctgaccaag ccatgcatga tttttatatg agatttgccc ctcaagtaat
    10741 cgaggcatcg ggcagtagtg ctggtggtgg aatgggtgca ggactgttag catttgcttc
    10801 agggaaaata gtctctggca tcgactacgt attggattta ctggagttcg ataaacgggt
    10861 acagcaagca gacctcgtaa tcgtaggcga aggacgtcta gatggacaaa gtctaaaagg
    10921 aaaagcgcca gttgggattg ctagaagagt accagagggc gttcctgtga tagctatttg
    10981 cggaagtgtt ggtaaaggaa gcgaaaaagc agcagatgtt ggcatttcgg ccgtttttcc
    11041 gatccttcct gcagctgtgc ctttacaaga agctttacag caagccactg ataatcttca
    11101 tcgcacagct aaaaacatcg catctttact tcaagtcgtt cattttcaag gaggtaatcg
    11161 atgaaaatca aagcagcact attcgattta gatggcgttc ttgtagacac agctcgttat
    11221 cattacgaag cgtggctggt tttagctaac cagctgtcca ttccattcac tgagaaagaa
    11281 aacgagcaac tgaaaggaat atccagaacc gagtcactag agcgattgct gtcatttgga
    11341 aaaatggaac agaaattctc agaaaaagag aaatcagcct ttgctgagca aaaaaacaat
    11401 ctatatcttc aagcgattca aaaaatggat gaaacatccg ttttgcctgg tgcaatagct
    11461 gttcttgagt acttgaaaaa aacaaacatc aagatcggat tgggatcagc aagcaaaaat
    11521 gcacgcctca ttttagaaaa aacgaatttg acaagttatt ttgatgtcct tattgacggc
    11581 acgcaagtat caaaagcaaa acctgatcca gaagtttttc tcaaaggagc acagcaattg
    11641 aatgtaccgc caaatgcatg cttggtcatt gaagattcag aagctggttg tcaagcagct
    11701 ttggctggta acatgcatgt attgggaata ggtgaaaaca taaatcttcc ttctgctgaa
    11761 tatgtgattc ctgatttaac cgtttttgat caagtacgtt cgttttggca tttatcagaa
    11821 gccgtatcac gtatataata gtttccgttt tttctgtcac aagcaaaacg ttatcctcct
    11881 tttggaaatc aaggtatgat gagaaaaaag ggggatttta tatgacaaaa aaagcagttt
    11941 tcgcaggcgg atgcttttgg tgtatggtca aaccatttga agagcagcca gggatcattt
    12001 ctgtgacatc cggttatact ggcgggcatg taccaaatcc aacttatgaa caagtaacaa
    12061 caggcacaac agggcataca gaagcagtag agatcgaata tgatccagaa gttatttctt
    12121 atgatcagtt ggtggaaatc tattggcagc aaaccgatcc gactgacgca ttaggacagt
    12181 tcgctgatcg cggtgattct tatcgtccag tgatctacta taatgatgaa gaagaacgaa
    12241 atattgcaga gcgttcaaaa caagcattgc aagacagcgg gcgattcgat aagccaatcg
    12301 tgacaaaaat cgaaccaaga agcgaatttt atcctgcaga agattaccat caggattatt
    12361 acaagaaaaa caagctccat tacaatcttt atcgagaagg ttctggacgt gcaggtttcc
    12421 ttcgtcgaca ttggggcgtc aagaaataag aagccgaaaa agctacttat ggaacaccgt
    12481 atatcagagg ttgtaaaacc agcattttga gctaatactg agggtcagct gattgaacac
    12541 catttagtgg agaggttgtg aacctgtcgc ctagctccaa gtcacaagga tttggtaaga
    12601 gaacactata tatcagaggt tgtgacaagt cgtttgtcac aacctttttt caatacctaa
    12661 gatgaaggac aatcagcaac gcactgctca aaaaaggaat gaaaggcagc ttttccaact
    12721 tttttttatg acagataaag taaacagaaa aaacggatat tccaaacaga ctagcaagaa
    12781 acaagatctg aagcaactct tctagagaca agccaccact ccaagaaagc atcaacagag
    12841 tatcgccagc tccgagatag gattgaccat agcgaaggca cacaccgcct acgataaacg
    12901 ttaacagcac actttgccat tggaagacgc cggcctgcaa ccaaaaaatc cataaaattg
    12961 cccaagaaca gaatagcgtg tgagaatgga ccatgaagta aaataaatcc atcaacgaaa
    13021 atagcaaagc cgaaagcatc catatgaacg tcgaaaaatt tcttgcggaa gaaaaagtaa
    13081 agaaccaggc aaaaagacca ccacataagg cttctgccaa caaatagtag aagggcaacc
    13141 gacacctgca atagcggcag cgaaatcgtt ggacgatgat cgagagaatc ggaatcaatt
    13201 cataccaaga aagcgaacga cagcatttga cacaatgaga tctaggataa ataatagagt
    13261 gacctaacgg aatccgctgg gcggttaaac ataaaaatga tccgatacag caaccaatga
    13321 taaaataaag taacacgcca tctccccctt ttcaaaacca agatacgcaa gcttaaacta
    13381 aatttttgta catacataaa atttgaggtg aaaaaatgaa cgtttcaatc ggattagata
    13441 tcggaaccac acaaaccaaa gcagttgctt tcacagatga agcccaagaa gcagcatccg
    13501 cttattttcg ctaccctttg atccaagaaa cacaagggat ggcagaacaa gatcaggaat
    13561 tgatctttca aggtgtctgc accgttataa aagaagtaat taaacagttg ccttcagaca
    13621 caatcattca atttgtctcc ttctcctctg cgatgcatgg cctgatattg ttggatgaag
    13681 agaaaacacc gttatctcga atgatcactt gggcagataa ccgagcagcg gacgaagcag
    13741 aaaaactgaa atataccgtg cttggcaaag aattttatcg gcagactggg acgccgattc
    13801 atccaatgac accattgaac aaaatcaagt ggctgcaaaa cagccaacct gaattattca
    13861 aaaaaactgc ttattttgtc ggtatcaaag actatatctt ttatagattg tttggcgaac
    13921 tgatcagcga ctacggaaca gcttcaggaa ctggatattt tgatatccat gaattccaat
    13981 gggcagaaga agtgcttgac tacttgaaca ttcgaataga gcaattgccg caagtctttc
    14041 cttccaccta tcaagtaaca ggtctaccat caaaaacagc agcagaacta ggtttgccaa
    14101 atgatatatc atttgttttg ggaggggcag acgggccgct ttctaatcta gggttaggtg
    14161 cttttggtga tacgatcgcc gctttgaccg taggaacgag cggagcactt cgttttatta
    14221 ctactcagcc tcagttccat ccagaaatgg agacgttttg ttatgttctg gatcagaaac
    14281 attgggtagt gggaggagcg actagcaatg gtgctggcat ctttgattgg gcagggcata
    14341 catttttaca ggaagtcgtt caatctgcta aagaaaaagg agaaaatcca tatgatgcac
    14401 tgctttcggc tatcgctttt gtgccagcgg gtgccaatgg tctgattttt catccctatt
    14461 tacttggtga gcgagcaccg ctttgggacg cagatgcgtt tggcagtttt atcggtttac
    14521 agcggcaaca tacagaaaaa gaaatgatga aggcgattct agaagggatc tgtctcaacc
    14581 tttatcggat tttaaaagga gtttgccagg aaaatcagct cacagagatc cgtgctacag
    14641 gcgggtttgc tcaatctccg atttttcgtc agatgatggc tgatgtactg ggttacccgc
    14701 ttgttttccc tactgtcaca gaagcatccg cattaggagc tgtgatttta ggctggagaa
    14761 gcatcggaaa gatccaaagt atcagtgaag cacaggaatt gatagaattg aaagagcaag
    14821 ttggagtaca agaaactgat cataaaaaat atcagaaaat ctatccatta tttgtttcga
    14881 cacaaaagca tttagcacag acttatcagc aatttgctca actgcgagaa gaattagctg
    14941 attctgaaaa atgattggct ttacggataa aaaagcgaac tgaaaatcgt tctcaggtaa
    15001 atgattttgt tgtacttttc attgaaagtc tactattatt ttaagtgtaa gaaaatgtta
    15061 ggaggcaaga aagatgaaat tccaacaaac caaagaagta ttaaatcaat tagttgcaga
    15121 tctaagccaa atgtctgttg ttgttcacca aacacattgg tacatgagag gaccaggttt
    15181 ccttacattg cacccaatga tggatgagtt catggatgat ttgaacgctc agttagatga
    15241 agtatccgaa cgcttgatca cattagacgg ttcaccttat tctacattgc gtgaatttgc
    15301 agataacaca aaaatccctg atgaaattgg ccgctgggat cgtactattc cagaacgttt
    15361 ggaaacactt gttgcaggct atcgttatct agcagatctt taccaaaaag gaatcgaagt
    15421 ttctggagaa gaaggcgacg atccaacaca agatatcttt atcgaattca aaacagccac
    15481 agaaaaacgt atctggatga tccaagctca tttgggcaaa gcgccagaga tcgacgcgta
    15541 aaaactgttg ttgaacttga ccgaaaaaga ggttgtgaaa tcagcgtttt gacctgagta
    15601 ttagagaaac aaacgtcaaa atagttcctc atattttgcc gttttttgag ctaataccga
    15661 gggtcagctg attgaacacc gtttaccaag aggttgtaag cctgccgttc agctccgagt
    15721 cacaagaaac aattttaaaa aacagcttct tatgttttat ggatattgtg acttgtgacc
    15781 gaggagttgg caggagaaca ccgtttattc aaaataaggg gttgtgacaa gacttttgtc
    15841 acagcctctt tttgtttttc attttgacag gaaacgaccc ttttatataa aagaaagaaa
    15901 agaagtaaag tttcatataa atttttcaat caagcagaaa aacgttgaca ttgactagat
    15961 tagcgggtaa tatgttaaat ggtattgttt gccccacaaa gagccccgga aactcgaagt
    16021 gtatgtcaaa catcagaact taaattggag gaaaataacg tgcgtacaac atatatggcc
    16081 aaaccaggcg aagtagaacg taaatggtat gtagtagacg caactgatgt tccattggga
    16141 cgtctttcaa cagttgttgc atctgtacta cgtggtaaaa ataaaccaac tttcacacct
    16201 catttggata ctggcgattt cgtcatcgtc atcaatgcag acaaagtgaa attaacaggt
    16261 aaaaaagcga ctgacaaaat ttattaccgt cacagcaact acccaggagg attgaaatca
    16321 atcactgctg gcgaattgcg tgctaaaaat tctcgtcgtt tgatcgaaac ttctgtaaaa
    16381 ggtatgcttc ctaaaaacac tttaggccgc aaacaattta caaaattgaa cgtatacggt
    16441 ggagcagaac atccacatgc agcacaacaa ccagaagttt tagacatcac aaacttaatc
    16501 taataggagg gaatttcatt ggcacaagtt caatatatcg gcacaggccg tcgtaaaaac
    16561 gcagttgctc gcgtacgtct agtaccagga actggcaaaa ttactgtaaa taaaaaagat
    16621 gttgaagaat acattccaca tgctgacttg cgtgaagtaa tcaatcaacc tttcgctgtt
    16681 actgaaacaa aaggcgcata tgacgtattt gttaacgtaa acggcggagg atacgctgga
    16741 caatcaggag ctatccgtca tggtatcgct cgtgcattgc tagaagttga tcctgacttc
    16801 cgtttagctc ttaaacgtgc tggcttactt actcgtgacg cacgtatggt cgaacgtaaa
    16861 aaaccaggtc ttaagaaagc acgtaaagct tcacaattct caaaacgtta atatccttta
    16921 tatatcaacg tttctggcac ttttcaatta tttggaaagt gcttcttttg tttttaagaa
    16981 cacatttaag aacacatatc aatcacactt attcaatcct ctctgaaaag cttcaaaaac
    17041 agccttatca actatatctg gtgtgttcac atatatcttt gttgtagaaa tatctgagtg
    17101 tgttaaagca tttgaaattt gattcatatc tgctccacct tgtctagcta tagttgcata
    17161 agtatgacgg agtttgtgtg gagataaatg aactaagtca ggatgccttc ttttaattga
    17221 attgattcga tagtttaggt aatcggcatg aacaggacaa tttacttctc cagatggttt
    17281 agtataagta aagagatact gatctaacgt ttgcttaatt tttaatttca acaattgatc
    17341 agcttgtacc gatttccatt cacttagcaa tgtcattaca acttctggta cttgaatgac
    17401 ggtatctttt cgaccttttg taaacttttt tctttgatat ttatctaaag tatgtttaag
    17461 acggaccgtt tgtttttcaa aatcaatata tttccattgc aaagcgtaag tctcactttt
    17521 tctatcacca agatacaggg taatcataaa aagtgtgtaa tcatgaaaag taagtaaatg
    17581 attattaaaa tcatcattta ctgcttctat ccaattagta agttcttttg atgatagtgc
    17641 ttgcttacct tcttgtattc ttttttcttc taaagcattt tttttaggtg cagtgatact
    17701 ttgaataatt ttagtagttc gattaaattc gatataattt agtattt
//
LOCUS       DACDSY010000055        17579 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDSY010000055 DACDSY010000000
VERSION     DACDSY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.055"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            59..808
                     /locus_tag="IWK44_001474"
     CDS             59..808
                     /locus_tag="IWK44_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9550010.1"
                     /translation="MVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQ
                     SGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENR
                     LTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQL
                     VETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPK
                     TGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="IWK44_001475"
     CDS             complement(1232..1402)
                     /locus_tag="IWK44_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9550011.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="IWK44_001476"
     CDS             complement(1596..1775)
                     /locus_tag="IWK44_001476"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550012.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2162..3274
                     /locus_tag="IWK44_001477"
     CDS             2162..3274
                     /locus_tag="IWK44_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9550013.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="IWK44_001478"
     CDS             complement(3352..3645)
                     /locus_tag="IWK44_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP9550014.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="IWK44_001479"
     CDS             3813..4127
                     /locus_tag="IWK44_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP9550015.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="IWK44_001480"
     CDS             4148..6127
                     /locus_tag="IWK44_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9550016.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="IWK44_001481"
     CDS             6140..6466
                     /locus_tag="IWK44_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP9550017.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="IWK44_001482"
     CDS             6482..7000
                     /locus_tag="IWK44_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550018.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="IWK44_001483"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="IWK44_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9550019.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="IWK44_001484"
     CDS             complement(8436..9941)
                     /locus_tag="IWK44_001484"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP9550020.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="IWK44_001485"
     CDS             complement(10033..10623)
                     /locus_tag="IWK44_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9550021.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="IWK44_001486"
     CDS             10802..12448
                     /locus_tag="IWK44_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9550022.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="IWK44_001487"
     CDS             12497..13216
                     /locus_tag="IWK44_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9550023.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="IWK44_001488"
     CDS             13695..14642
                     /locus_tag="IWK44_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9550024.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="IWK44_001489"
     CDS             14654..15574
                     /locus_tag="IWK44_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9550025.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="IWK44_001490"
     CDS             15615..17072
                     /locus_tag="IWK44_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9550026.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..>17579
                     /locus_tag="IWK44_001491"
     CDS             17417..>17579
                     /locus_tag="IWK44_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9550027.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEY"
BASE COUNT         5516 a         3012 c         3587 g         5464 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagaaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtata
//
LOCUS       DACDSY010000056        17344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDSY010000056 DACDSY010000000
VERSION     DACDSY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.056"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..368
                     /locus_tag="IWK44_001492"
                     /pseudo
     CDS             1..368
                     /locus_tag="IWK44_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            548..1489
                     /locus_tag="IWK44_001493"
     CDS             548..1489
                     /locus_tag="IWK44_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP9550028.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1558..2289
                     /locus_tag="IWK44_001494"
     CDS             1558..2289
                     /locus_tag="IWK44_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP9550029.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2433..4613
                     /locus_tag="IWK44_001495"
     CDS             2433..4613
                     /locus_tag="IWK44_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP9550030.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4633..5115
                     /locus_tag="IWK44_001496"
     CDS             4633..5115
                     /locus_tag="IWK44_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP9550031.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5131..5892
                     /locus_tag="IWK44_001497"
     CDS             5131..5892
                     /locus_tag="IWK44_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9550032.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6023..7357
                     /locus_tag="IWK44_001498"
     CDS             6023..7357
                     /locus_tag="IWK44_001498"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP9550033.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7785..8270
                     /locus_tag="IWK44_001499"
     CDS             7785..8270
                     /locus_tag="IWK44_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP9550034.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8263..8946
                     /locus_tag="IWK44_001500"
     CDS             8263..8946
                     /locus_tag="IWK44_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP9550035.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9072..11717
                     /gene="polA"
                     /locus_tag="IWK44_001501"
     CDS             9072..11717
                     /gene="polA"
                     /locus_tag="IWK44_001501"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP9550036.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11762..12598
                     /gene="mutM"
                     /locus_tag="IWK44_001502"
     CDS             11762..12598
                     /gene="mutM"
                     /locus_tag="IWK44_001502"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP9550037.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12562..13191
                     /locus_tag="IWK44_001503"
     CDS             12562..13191
                     /locus_tag="IWK44_001503"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP9550038.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13243..13575
                     /locus_tag="IWK44_001504"
     CDS             13243..13575
                     /locus_tag="IWK44_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9550039.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13887..14378
                     /gene="nrdR"
                     /locus_tag="IWK44_001505"
     CDS             13887..14378
                     /gene="nrdR"
                     /locus_tag="IWK44_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP9550040.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14401..15774
                     /locus_tag="IWK44_001506"
     CDS             14401..15774
                     /locus_tag="IWK44_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP9550041.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15774..16700
                     /gene="dnaI"
                     /locus_tag="IWK44_001507"
     CDS             15774..16700
                     /gene="dnaI"
                     /locus_tag="IWK44_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP9550042.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16829..17269
                     /locus_tag="IWK44_001508"
     CDS             16829..17269
                     /locus_tag="IWK44_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9550043.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5908 a         3032 c         3820 g         4584 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tcggaaaaag atcgtcaaga aatggatggg acgcttataa
      121 ttgaagtagg agttgaggac agtcaaacct catttctgtt atatgatcga gcatttgttg
      181 agtcccattc acctggcaca gctactagtg ccccatcttt gatgttttac agtaaagata
      241 tctttggact atataaaaaa atgcagacgc aatcagtcac attaggtgat atggtacagc
      301 ttggagaaga atatgtcttt aactttgctg atcccgatgg caactacttt gcggttacag
      361 gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa caggaaagcg aagtcttttt
      421 aattcaaata ataatattat gtaaacaaaa aactttccat gcgtaattat acacaagcaa
      481 ataaatcgtc aaatgcgaac gccttcttta tactgtttag gaacgaaaag aaggaaggaa
      541 gaaatacatg gattatcaag tactgcttta ttacaattat acaaaaatcg aagaaccaga
      601 ggaatttgcc aaaaatcatt tagccttttg caaatctttg gatcttaaag gtcgtattct
      661 agtagctgaa gaaggaataa atggtactgt gtctggaacc gttgaaaaca cgactgccta
      721 tatgaaagca atgaaagctg atcctcgatt tgctgatacg atgttcaaga tcgatccaag
      781 cgaaggacat gcctttaaga agatgtttgt aagataccgt ccagagcttg taagtttaaa
      841 gctagaagag gacattgatc caaaccaagt gacaggtgcc tatctttcac cgcaagaatt
      901 taaagaagct attttagatg aacagacagt ggtgattgat gcacggaatg attatgaata
      961 tgacttggga cattttcgag gggctgtacg cccagatata cgcagttttc gagagcttcc
     1021 agactggatc cgcgagcaca aagaagaatt tatggaaaaa cgggtagtga cctactgtac
     1081 tggtggcatt cgttgcgaga agttttctgg ttggcttgtc cgtgaaggat tcaaagatgt
     1141 cggacagctg catggcggta ttgctactta tggtaaggat cctgaagtcc aaggagaatt
     1201 atgggacggc aagatgtatg tgtttgatga acggatcgct gtcgatatca accaagtaga
     1261 taaacaaatc gtcggtcgcg actggtttga cggaacacca tgtgaacgat atatcaattg
     1321 tagcaatcct gagtgcaatc gtcagattct tgcttccatt gaaaatgaag aaaaacattt
     1381 aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat cgctatgttc aagaccatgg
     1441 aatctctcca gaagaagcag aactgcggtt tacagcttta attaaataga aaacaatagt
     1501 tttgattgtc gaccatttcc aatatgcgct atactagcgt tgaggatggt gatccacatg
     1561 aaatcattga tttatgctat tggggatgtc catggcaaac atgagctttt tgaaaaactt
     1621 attcactatt atgatccaaa gatccatcag cttgttttat taggggatct caatgatcga
     1681 ggtcctcgta caaaagaatg ttttttatta ggaaagcaat tagtcgaaga aacaggagcc
     1741 atttacctcc ggggaaacca tgaagaatat tttttgcaat ttctgcaatc accggaggac
     1801 tggtatgcgc cttatatgag aaatggtgga aaagaaacgt ttgaaagcct gattcaccca
     1861 ggggcatcgg aggaatactc cccaactgaa atctcgatga tgatccaatc gagatattca
     1921 gaactgatta aatttttagt ccagcgcccc ttgtattatg aatgggaaaa ttatcttttt
     1981 gtccatgccg gtgtggattt gacaaagaaa gactggcgta agacaagtct aagagacttt
     2041 atttggataa gggaaccatt tcatgaaggg aaaaacaata caggtaagac aattgtattt
     2101 ggacatacga ttacaccaat gctccatgga gatatgcaga caacagacct ctggattcag
     2161 gatcataaaa tcggaatgga cggtggggct gtattcggag ggtcattgca tggggttgtt
     2221 tttgacaagg aaggtattct tcaagatatt gaatatcaga acaacgaagg accttggcat
     2281 ggaatctgac taatggaaag aatgaaggag ctgtgacttt gttgcggctc tttttatttt
     2341 tatagagaat attgtttctt tcagtagttt aacccaggat aaaaagtatg atagaataaa
     2401 gaagcaattt ttagcagaaa agaggcattt taatgacgga aaatttgaac cagacgatca
     2461 ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa gcagttgact actgtactta
     2521 atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg ttaccgaaaa gaaatgactg
     2581 gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg gtatgcctat cttgaaaact
     2641 tagaaaaacg taaaactgaa gtgattcggc taatcgatga acaagggaaa ctgactcccg
     2701 agttagaagc agaaatcacc caagccgtga aaatgcaaca agtggaagat ttgtatcgtc
     2761 catacaaaca aaaacgacgg acaaaagcaa cgattgctaa agaaaaagga cttgaaccac
     2821 tcgcgaagtg gctgatgcag cttacagatg gagaggtgca atctgaagca gaaaaatata
     2881 ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt gcacggtgct catgagatta
     2941 ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg gattcggtct tatacgtata
     3001 acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc agatgaaaaa ggcgtatatg
     3061 aaatgtatta tgactttgca gaacccgtcc ataaaatggt ttctcatcga atattagcta
     3121 ctaatcgtgg agaaaaagaa gatattctga aagtattctt tcaagtagat gaagcagcaa
     3181 ttttagctta cttggatcgc caaatagtca aaaatcctgc ctctccttct tcttcattcg
     3241 tcagagaagc ctatcaggat agttataaac gctttattca gccagcaatc gagcgagaac
     3301 tgcgcaatga attgactgaa aaggcagatg agcaagcaat cgctattttc ggcgaaaacc
     3361 tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt tgtcctggga tttgatccgg
     3421 cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac agggaaagtt ttagcaatcg
     3481 aagttattta tcctcataaa cctgcagcgc aagcaaaaag agaagctgct ggaccagctt
     3541 ttatccaatt aattaataaa tatcaagtag atatggtagc tatcggaaat ggtacagcca
     3601 gtcgggaatc tgaacttttt gtggctgagc agttgaaatc cgccgaccat aaaacctatt
     3661 acgctatcgt caacgaagca ggcgcttctg tttactccgc tagcgaaatt gcacgaaaag
     3721 aatttccgca tctacaagtt gaagaaagaa gtgctgtttc tattgcccgt cgcctgcaag
     3781 atcctttagc tgaattagtc aaaatcgatc ctaaagctgt cggagtaggc cagtaccaac
     3841 atgacgtttc tcaaaaacgt ttggctgaac aattggattt tgttgtggaa acagcggtta
     3901 accaagtagg tgtggatgtg aatacagcaa gcccgcagct cttgcagcat atatcaggat
     3961 tgaataaaac aactgcccaa aacattgtca tttatcgtga agaaaacgga gaattcaccg
     4021 cacgaactca attgaaaaaa gtgccacgct taggaccaaa agcatatgaa caagcaatcg
     4081 gattcttgcg agtccctggt gggaaaaaca ttttggacaa cacaggtatc catcccgaaa
     4141 gttattctat tgcaaaagaa atattaactt cagtccaact ttcagaaaaa gagcttggta
     4201 cagaagaagc agtagaaaaa ttgacacgat tatccgtaga gaaactggct gaaagcctat
     4261 cagtcggaga agaaacgtta gccgatattt tagctgggct gacacaacct ggacgagata
     4321 tgcgtgatga gatgccagcg cccttattac gtacagatgt attgagtatg gaagacttga
     4381 aaccagggat ggaattgact ggaacggtcc gtaatgtcat tgacttcggc gcatttgttg
     4441 atatcggtgt gaagcaagac ggacttgtcc atatatcgaa actcagcaaa aaattcgtca
     4501 agcatcctac agatgtcgtg tcagtaggag atatcgtaac agtttggata gaacaagtcg
     4561 atacgaaaaa aggcagaatc agtttgacga tgctttcccc ttatgaagag taacaatgag
     4621 gagtaacaaa aattgacaca agaagaactg cagcagttag tagaagagat atcattaaaa
     4681 agcttccagc ttcctttcaa gcaccaagca atgttcaatc cacgcttacg tacaactggc
     4741 gggcgctatc atttaaacga tcatcattta gattttaatg cgaagctatt tgaagagtca
     4801 gacgaagaga cgattgcagg aatcatcaaa catgaacttt gccattatca tcttcatcta
     4861 gctggaagag ggtatcagca taaagatatg gatttcaagt atcttttgaa aaaaacagga
     4921 gggttgcgct atgcaccagc cttgacgaag aaacagataa agatggagta ttatcgatgc
     4981 caagattgtt ctgccacgat ctgccgcaag agaagaatcg atgtacgcaa atatcgttgt
     5041 ggaagatgtc atggagaaat caaatggatt gagacaaaaa gagaagactt taatgaagca
     5101 caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa gtgagcagag gaatctaaaa
     5161 gactggaatc agctattaaa ttttttagcc gcacctgaag agggaaattt tgaggaactg
     5221 acggcaccta cacttatttt agctggaaac tgtcttccta tcttagctga taaagcagca
     5281 caaatgtatt taaatggcca agtagatcaa ttgtttttgg tcggaggggt ggggcatgcg
     5341 actagaatcc tttatgagaa ttttgaaaag caaggatttc attttgaaga tggcatgagt
     5401 gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg atttgcccga taaggctttt
     5461 ctgatagaat cgaagtctac aaattctgga gagaacgcca ctttttcact ggagattctt
     5521 cattccttag gagctgtacc agaaaaaatc ttattgatga atgatccgac actgcaacgc
     5581 aggactcgag caacttttga gaaagtatgg caaaatgaac aaactatttt cgcgaatgct
     5641 gtgccatttg tgccggaaat ccttcacttt tctgaagaga tcatctttac agcaaaagaa
     5701 ctgaatcatc aatggcctaa ggaatatttc catgcgctag tattaggaga aatggaacgt
     5761 ctccatgacg atgagaacgg ctatggtccg aaaggaaaag attttattcc tcacattgat
     5821 atatcagagg aagtttggtc ttcttacacc agaattaaag agtcaataaa aacggatttt
     5881 tcccgtactt gaccatttta gcaagcgaat cctcctttcc aaataaattt ggttgaaatt
     5941 ttcaatcgtt tataagataa tgaggagcgt gttgaatgta atagctaaaa gaatggacgc
     6001 tagataagag aagaggattc aaatggaaaa tcaaaataaa ttgtatcatt ttgttggtat
     6061 caaaggttca ggaatgagct ccttggctct tgttttacat gaacaaggtc taaatgtcca
     6121 aggatcagac attgaaaaat atttctttac acaaagagat ttagaaaaag caaatatcac
     6181 gattctgcca tttaacgcag ataatgtaaa accagggatg acgatcattg caggaaatgc
     6241 atttcctgat tcacatgaag aaatccagcg agcaaaagaa ttaggtttgg aagtgattcg
     6301 ctatcacgac tttattggtc attttatcca aaattatacg agtatcgctg taacaggctc
     6361 tcacggaaaa acaagtacga ctggactgct ctctcatgta ttgagtggtg tccgtccaac
     6421 aagttatcta attggagatg gaacaggaca tggtgatccg caagcggaat tcttttcatt
     6481 tgaggcctgt gaatatcgcc gtcatttcct tgcctattca ccagactatg caatcatgac
     6541 caatatcgat tttgatcatc cggattatta tacaagtatc gacgatgtat ttacagcttt
     6601 ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg tatggtgacg atgcttattt
     6661 acgcaaatta aaagcaaatg tgccgattta ttattatggt gtcacagaaa acgatgatat
     6721 ccaagcacga aacatcgaac gcacaacttc tggttctgct tttgatgtct atcatgggga
     6781 tgaatttgtt gggcacttca ctgtaccagc attcggtaaa cacaatatct taaatgcttt
     6841 aggtgttatt gcagtagctt atttcgaaaa actggatctg aaagaagttg cagaagaaat
     6901 gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag atcgtagcag acatgaccgt
     6961 agtcgatgat tatgcgcatc accctgcaga gatcaaagca acgattgatg gcgcacgcca
     7021 aaaatatcca gataaagaaa taattgcagt tttccaaccc catacgttta cgcgtacgat
     7081 tgcattgatg gatgaatttg cagaagcact tgatttagct gataaagttt acttatgcga
     7141 tatttttggt tcggctcggg aagaacaagg aaacgttaag attgaagacc ttggagcgaa
     7201 aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta tctccattgt tagattatca
     7261 tgatgcagta gtaatcttca tgggtgctgg agatgttcaa aaatttgaac aagcgtatga
     7321 aaaactttta agcagcacga cgaaaaatgt actgtaatac aattactgca gtgtccttat
     7381 aaaaatagaa agagggggag tggcaaaaat cttgccaagc cccctttttt gcaacttatg
     7441 ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc caatcaaatc aaaaacatga
     7501 gaagctgttt ttcttggctg ttcttattac ttgatgcagg gcagcttttt cacgacctca
     7561 gataaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaaat gaaaaatatg
     7621 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7681 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7741 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7801 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7861 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7921 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7981 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     8041 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     8101 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8161 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8221 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8281 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8341 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8401 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8461 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8521 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8581 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8641 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8701 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8761 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8821 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8881 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8941 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     9001 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     9061 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9121 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9181 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9241 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9301 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9361 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9421 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9481 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9541 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9601 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9661 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9721 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9781 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9841 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9901 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9961 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
    10021 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
    10081 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10141 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10201 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10261 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10321 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10381 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10441 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagataaa aaccaagaag
    10501 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10561 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10621 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10681 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10741 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10801 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10861 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10921 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10981 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    11041 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    11101 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11161 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11221 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11281 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11341 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11401 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11461 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11521 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11581 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11641 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11701 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11761 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11821 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11881 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11941 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    12001 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    12061 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12121 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12181 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12241 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12301 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12361 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12421 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12481 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12541 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12601 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12661 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12721 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12781 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12841 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12901 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12961 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    13021 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    13081 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13141 aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13201 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13261 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13321 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13381 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13441 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13501 gaccacattt ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13561 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13621 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13681 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13741 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13801 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13861 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13921 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13981 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    14041 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    14101 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14161 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14221 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14281 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14341 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14401 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14461 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14521 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14581 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14641 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14701 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14761 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14821 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14881 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14941 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    15001 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    15061 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15121 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15181 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15241 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15301 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15361 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15421 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15481 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15541 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15601 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15661 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15721 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15781 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15841 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15901 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15961 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    16021 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    16081 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16141 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16201 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16261 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16321 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16381 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16441 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16501 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16561 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16621 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16681 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16741 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16801 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16861 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16921 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16981 atttgtcgga acgattgaaa gtattactca agtagaagca gaagaagaaa cgcttcagtt
    17041 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    17101 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17161 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17221 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17281 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17341 tggg
//
LOCUS       DACDSY010000057        17094 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDSY010000057 DACDSY010000000
VERSION     DACDSY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.057"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            12..218
                     /locus_tag="IWK44_001509"
     CDS             12..218
                     /locus_tag="IWK44_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550044.1"
                     /translation="MKQKVVGEVISKLENCVVICVDEYHDVDRNEILEKCGKVVAKYT
                     HVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(580..819)
                     /locus_tag="IWK44_001510"
     CDS             complement(580..819)
                     /locus_tag="IWK44_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550045.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1105..1419
                     /locus_tag="IWK44_001511"
     CDS             1105..1419
                     /locus_tag="IWK44_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550046.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(1853..2219)
                     /gene="ssrA"
                     /locus_tag="IWK44_001512"
     tmRNA           complement(1853..2219)
                     /gene="ssrA"
                     /locus_tag="IWK44_001512"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2390..3163)
                     /locus_tag="IWK44_001513"
     CDS             complement(2390..3163)
                     /locus_tag="IWK44_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9550047.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            3307..3657
                     /locus_tag="IWK44_001514"
     CDS             3307..3657
                     /locus_tag="IWK44_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9550048.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            3638..4354
                     /locus_tag="IWK44_001515"
     CDS             3638..4354
                     /locus_tag="IWK44_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9550049.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            4354..5802
                     /locus_tag="IWK44_001516"
     CDS             4354..5802
                     /locus_tag="IWK44_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9550050.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(5872..6381)
                     /locus_tag="IWK44_001517"
     CDS             complement(5872..6381)
                     /locus_tag="IWK44_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550051.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(6371..7096)
                     /locus_tag="IWK44_001518"
     CDS             complement(6371..7096)
                     /locus_tag="IWK44_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550052.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(7319..9439)
                     /locus_tag="IWK44_001519"
     CDS             complement(7319..9439)
                     /locus_tag="IWK44_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9550053.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            9669..10847
                     /locus_tag="IWK44_001520"
     CDS             9669..10847
                     /locus_tag="IWK44_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP9550054.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            10863..11621
                     /gene="aroD"
                     /locus_tag="IWK44_001521"
     CDS             10863..11621
                     /gene="aroD"
                     /locus_tag="IWK44_001521"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP9550055.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            11644..12129
                     /gene="ybaK"
                     /locus_tag="IWK44_001522"
     CDS             11644..12129
                     /gene="ybaK"
                     /locus_tag="IWK44_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP9550056.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(12200..13453)
                     /locus_tag="IWK44_001523"
     CDS             complement(12200..13453)
                     /locus_tag="IWK44_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9550057.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            13571..14920
                     /gene="gdhA"
                     /locus_tag="IWK44_001524"
     CDS             13571..14920
                     /gene="gdhA"
                     /locus_tag="IWK44_001524"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP9550058.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            15032..16378
                     /locus_tag="IWK44_001525"
     CDS             15032..16378
                     /locus_tag="IWK44_001525"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP9550059.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(16686..17072)
                     /locus_tag="IWK44_001526"
     CDS             complement(16686..17072)
                     /locus_tag="IWK44_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9550060.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5603 a         2989 c         3411 g         5091 t
ORIGIN      
        1 aacctattgg cttgaaacaa aaagtggttg gagaagtaat cagtaaatta gaaaattgtg
       61 ttgtgatctg cgtagacgaa tatcatgacg tcgatcgcaa tgaaatatta gaaaaatgcg
      121 gaaaagtagt ggcaaaatat actcacgttt atgagttgga aacaacagaa tatctatata
      181 actatagcaa caaatatgaa catactttag tttcataaag atttaaataa agtgccttca
      241 aaagcgagaa gaccaagctt ttgaaggcac tttatttagt tggcagtgat tgttcatgat
      301 ttttgattta ttcaaaaggt tcgcctagag aatctgtctc tgtaacgagg cctggtggag
      361 aaactcgata aaaacctata ctgccagaac tgtcctattt tcggatagac aaactagtag
      421 ttttaaaaag atcatccgat ccaatcattt caaaaaatgc aaaactgata tcaggatcat
      481 ttagctcagg cttgttatcg taaagcattt gtaaaggttg catttagtta ggagtaaacg
      541 cgaaactatc atcattattt gctgaagaag atgaagaagc taattcagag tttgtttgaa
      601 ataaaggaga cgtgctgaca gtttctttac cctgtttttt gcagaggtag aaacttttat
      661 atcggaagtt tctaccggag aatttttctc atttgtagaa attgttgaat cgatactatt
      721 cgaaatttgc gtatctgaag gtatgttctt tgatgcagaa gtcccacaag cagtaagtaa
      781 caataaattt gataaactga taaacagaat tcttttcatt atgtacacac tccttatagt
      841 atcgtttagc atacacctat gctcagtatt aggcaaaatc aatgcaaaac tatcaagtct
      901 tttatagtat ctattagtct tagtaataca tatatggaga gggtgatatc ccagttcaaa
      961 ccagatacat tggttaccat ctgtattaac gattatgcct ctaaacgctt tttttatgaa
     1021 aaaatttata agtgaaaacg taggtatgat attatctaga ggtaattttt ttgggtattt
     1081 aaatagttgc taggaggata gcttttggat ttttacagta attttatctt aataatagca
     1141 atattattac ttctaaatat ttggtttttt gataagagta gaaatgcagg gataggtttt
     1201 agaaccaaac gaagtacgtc ttctgaaaaa aagtgggtat attcacagac tattttttac
     1261 ggaggggtta tttcaatcag tttactttct tcaacacttt attcttttaa tgttatagac
     1321 gtttccatgt caaattttat atctattatt ggaatcctta tttctgcaat tattacacaa
     1381 ctacttcttg tgtttgaaga gaagtcaaaa aataattaaa gcaattatgc cttttagtac
     1441 aggaattatt acttcttaca aatgtatagc gaatgtgaag cctgctgttt taattgttct
     1501 agaaaaactt ttgagtgaac aattatttta tgttaggatg aattaatttc tctagagttt
     1561 ataattaaaa aacttatttg aaggaacatg cttaaaccat aattgtgttt ttccttccat
     1621 cagcataaaa gttcacctca aaaagagtgt acgaacaaaa gttctttttg taaaccttaa
     1681 aatagatttt cctgaatacg tattggggat ttctaaatgt ttgaatgttt ttttgaatgc
     1741 aaaatcaatg caaaaaattt tgtatttctc tacttttctc tccttgacca aaataaaaaa
     1801 acgttgattt aacaacgttt ttcatacatg tttatctgtc tcaaccatac atatatggag
     1861 acggcgggag tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc
     1921 aactcattta aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc
     1981 tgatgttctc tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga
     2041 cccttactcg agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct
     2101 aaagcgtaag agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag
     2161 acgtaaccta cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg
     2221 ttagctaaca agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata
     2281 aattcaataa aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag
     2341 aaaaaataag cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat
     2401 caaaatactt caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag
     2461 aaagttcttt taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca
     2521 acaaggcaaa atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa
     2581 gttctttttg cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga
     2641 tcgagtaagg accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt
     2701 cacctgattg agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat
     2761 caatttccgg aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac
     2821 cggtacacca gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat
     2881 gatcatacat tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt
     2941 ctactcgaat gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga
     3001 tagagtggat ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt
     3061 ttaatgcgtt catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg
     3121 ttccatcaag atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt
     3181 tattggaata agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt
     3241 cctgaacata ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg
     3301 gaaagaatga tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg
     3361 attctgccct caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa
     3421 acagacgctc attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc
     3481 ttactgatgg cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg
     3541 attgaaatgc ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa
     3601 ttacagaaat atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga
     3661 taatcaatct gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac
     3721 tttcaagtat gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga
     3781 tatgattaca ctagacctga atttgcccac actggacggt atgctagtag gaagagagat
     3841 ccgcaaagtt tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga
     3901 tcaggtgatc ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct
     3961 gacgcttatt gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga
     4021 agaagcagca gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac
     4081 gaaaacacga gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt
     4141 cgatctattg tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct
     4201 tgaattagtc tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa
     4261 gaaattacga caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt
     4321 cggctacaaa ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta
     4381 gctttttgcg gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg
     4441 acaaaacaaa cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt
     4501 gaaaagacaa cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt
     4561 caaaatgcat tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt
     4621 atcgataaaa acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta
     4681 acagctgatc aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa
     4741 aatattttag gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat
     4801 gaattctatg gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt
     4861 ccaattacac tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc
     4921 agctatgctt ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa
     4981 gaagtgacga atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat
     5041 gagttagcag atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt
     5101 gaagaacaag aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca
     5161 cctcttacga caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat
     5221 cagcgagaaa acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg
     5281 aatgaaaact tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa
     5341 ttcaatggaa cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca
     5401 gcaggagata cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac
     5461 cgatttgtcc aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc
     5521 caaatcacga ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg
     5581 ggaattggaa tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat
     5641 ccttcaagaa aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa
     5701 cttgtacgct tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa
     5761 ttcgctgttc gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa
     5821 gatcatgaat cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc
     5881 gggctctaaa cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa
     5941 gataccttga ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc
     6001 caatacacta agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa
     6061 ggcatgaagt aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt
     6121 aagcaaggaa ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc
     6181 tttttccggt acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca
     6241 atttttttgg atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg
     6301 ctccaatatt ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg
     6361 aatcaaatat tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa
     6421 aagtgaaagg gaatagacta agaatgagac agcatccact gagcccaata agagtttggg
     6481 caaatccctt aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag
     6541 tctgttgatc catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg
     6601 gcagttcagt agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg
     6661 actggaagct tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca
     6721 gctgccagaa aataaaaagg aataatttgg ttttagaaag accagtcagg cgccaattga
     6781 tcaattcctg cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa
     6841 atagaaaaaa taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg
     6901 gaagaagctc tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg
     6961 ctaaagccga ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc
     7021 ttgaaaataa acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat
     7081 agcggattat tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa
     7141 gtatgaaatt cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa
     7201 gaggttgtga agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt
     7261 attcgaaaag aggagcagcg acaataattt tgtcgctgct ccttttaaat ttattttgtc
     7321 attcggaaga acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat
     7381 caacagttga ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt
     7441 tttcagcttc ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg
     7501 ggtctaatcc actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa
     7561 cagctaactg tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag
     7621 taggttcagt aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat
     7681 attttggtgt aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga
     7741 atagatcttg tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg
     7801 taggcaaggc atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag
     7861 ggatatggat catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag
     7921 aacttgtttt tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat
     7981 ggtcaccgta aaggacgatg actgaattat catacaagcc gctttctttc aaataattga
     8041 agaattcttg tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt
     8101 catcatttgt attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat
     8161 aatggttaga tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt
     8221 gtacggattg ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat
     8281 taacatcata ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt
     8341 ttcgattcca gaatgtacca gcgttcccat ggaataccgc actagtgtaa ccttgtgttt
     8401 gacttaaaat atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg
     8461 agccttgatt caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc
     8521 ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac
     8581 tattgatgaa aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga
     8641 tcaggaattg ctggaagctt tctaaatgga tataaatcac attttttcct ttagcgagcc
     8701 cgaacaaatc gtcatttggt gctgcataat gttcttttac ataattttct acttctttca
     8761 tatcattggg acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat
     8821 aaaccatgaa agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag
     8881 tcagcaattc tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg
     8941 ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt
     9001 tttttgcaat cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg
     9061 ggcgaaacag tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa
     9121 tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa
     9181 gcaatgtcaa aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga
     9241 tataaagagc aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa
     9301 agtattgaat gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt
     9361 tcaaccaaag taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag
     9421 gtgtttttat acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtca
     9481 tgaaaccatg ttacacagct cacttattct catttatttt actagtatga caatatagtg
     9541 tcaattgaaa ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa
     9601 atgagcagta agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag
     9661 gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc
     9721 cactgatcca aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat
     9781 tagttgattc gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa
     9841 tcggttgggt attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt
     9901 ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc
     9961 gtatattcaa tggagaagga gacggaatcg gcggtctgat tgttgattat tatgcaggtt
    10021 atgctgtttt ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag
    10081 cttttcgtga aatctacccg gaaatcgtag gaggctatga gaaaattcga tttgaatcag
    10141 taaatctgcc tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa
    10201 aagaaaacgg agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt
    10261 tagaccaaaa agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt
    10321 tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg
    10381 aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag
    10441 tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca
    10501 aatacgccaa aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg
    10561 ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata
    10621 gtctggatat tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct
    10681 cgatagaaca attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg
    10741 aacaacttgc tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag
    10801 gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca
    10861 gaatggtttg ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc
    10921 ttatcggggc cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg
    10981 ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag
    11041 cagaaacatt ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc
    11101 gtaccttagc tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg
    11161 aacatgtgat acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa
    11221 gtctaggaaa aagactgata gaggaaatca agtctttaaa cattccttta attatcagta
    11281 gtcatgattt taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg
    11341 aacattttgg agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt
    11401 tgcggttgat ggagttgact agacgggcaa atgcttttgt ctccatgcct attattacga
    11461 tgtccatggg aaatcttggt aaaatttctc gtctggctgg cggaacgacg ggttccgtta
    11521 tgacatttgg ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag
    11581 cattgaagca aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat
    11641 tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag
    11701 attccctata cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc
    11761 gctgaacagc ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat
    11821 gtgacggggc ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta
    11881 gcaaaagttt cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg
    11941 accggataca tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat
    12001 ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta
    12061 cagatggagt tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata
    12121 aaacaataaa tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc
    12181 cttctttctt ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg
    12241 tagcgaaagc cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc
    12301 ccgcaaaata cagaccatca atgattttac tctccaatgt tttaggataa atttccttta
    12361 gagaaacccc tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata
    12421 gcttaaactc tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc
    12481 ctgtcagatg atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata
    12541 taccatgcca agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat
    12601 gaataagctc ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc
    12661 ccgttttctc taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa
    12721 taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat
    12781 ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat
    12841 acggttcatc cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca
    12901 ctcgcttcac gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac
    12961 cagttgttaa gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa
    13021 ttttatgttc tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta
    13081 gttctttcag acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa
    13141 acatccgtcc atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga
    13201 tatcgaaatt attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat
    13261 gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca
    13321 tcagtagttt ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt
    13381 cagctgcact gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat
    13441 acagtttttc cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga
    13501 aaaaaaatgt tttgagccaa aagagaaaat gagctaaaat gagggaagat aagcaaagga
    13561 gcgtggaacg atgacaaacg caacagaata tgttcaagca attcaagaaa agctacatca
    13621 aaaggatcaa gggcaagaag aatttttaca agcaatcgat gaattcatgc ccacggttat
    13681 gtcttttttg gacactcatc ccgaatatat cgaaaagaat atcttagaac ttttaacgga
    13741 accagaaaga gttattcaat ttcgagtgcc ttggcaagat gatgaaggaa actggcgagt
    13801 aaatagaggg taccgtatcc aatacaactc agctatcggc ccttataaag gtggattgcg
    13861 cttccatcct agtgtcaatc taagtatctt gaaatttttg gcttttgaac agattttcaa
    13921 gaatagttta accggtctgc caatcggtgg aggaaaaggc ggaagtgact tcgatcctaa
    13981 aggaaaatct gataatgaga tcatgcgttt ttgtcaaagt ttcatgctgg aattgtcaaa
    14041 acatatcggc ccatcgattg atgttcctgc gggagatatc ggtgtaggag ctagagaaat
    14101 cggctatttg ttcggcgaat ataaacgatt gaaaaaatat gatactggtg tgttgacagg
    14161 taaacctttg gatttttggg gaagtaagat acgtacggaa gctactggtt atggattagt
    14221 gtattatgtc aaacatctat tgaacgaaga aaaagattca tttaatcaaa aaacagtttt
    14281 tgtttccgga agcgggaatg tagccattta tgcaatcgaa aaagtgcaag agctaggcgg
    14341 gaaagttgtt acatgttcag attctagcgg gtacatctat gatccagaag gaatagatgt
    14401 gaaactattg aaagaagtca aagaaatcaa tagacaacgt ttgacggaat acgcaaacca
    14461 acgtccttct gctatttatc atgctggaga atctgtttgg acattgaaag aaaaagcgga
    14521 tatcgctttg ccatgtgcta ctcaaaatga aatagacgaa agcttggccg aaatcttagt
    14581 agaaaatggg atcaaaatcg tagcagaagg cgctaatatg ccatgtacag taaaagctgt
    14641 ttctgttctt catgaagcag gcgtgtggta ttgtccagga aaagctgcta atgctggagg
    14701 agtagcagta tctgctttag aaatgagtca gaacgtccag cgattgactt gggaaagaga
    14761 gcaagtggat gagcagctgg atcaaatcat ggaaacgatt taccatacgt gtcgccaaac
    14821 agcagccgag tacggcgacg aaaaaaatct gcttcttgga gcaaatgttg ctggatttga
    14881 aaaagttgcc aaagcaatgg cagcagaagg tttagtttga aaaatgaaac tttcttggaa
    14941 atattgtcag cattttgttt atttgcttga aaaataataa aaaaaaacgt aaagtattac
    15001 ctgtagagta aacctataag gaggagaaca aatggcacac attcattttg actattcaaa
    15061 agtagcaccg tttgtcaacg aacaagagtt agggtacatg caaagccaag taacagcagc
    15121 ccacaacgaa ttacgggaag gtactggcgc aggaagcgac ttccgcggct ggatcgattt
    15181 accaacgaac tatgacaaag aagaatttgc acgtatcaaa gaagcagcta aaaaaatcca
    15241 atctgattct gaagtattag ttgttatcgg tatcggtggt tcttatttag gcgctcgtgc
    15301 agcgatcgac ttcttgaatc acacattctt caatttatta gatagcaaca caagaaaagc
    15361 accacaaatc ttctttgccg gaaattcaat cagctcaaca tatattgctg acttgatcga
    15421 agtaatcggt gatcgcgact tttcagtaaa cgttatttca aaatctggaa caactactga
    15481 accagcgatt gcgttccgtg tattcaaaga gcttcttgtt aagaaatatg gacaagaaga
    15541 agcaaacaaa cgtatctacg ctacaacaga taaagcaaaa ggtgcagtta aagtagaagc
    15601 agatgcagaa ggttgggaaa ctttcgtgat tcctgatgac atcggcggac gtttctctgt
    15661 attaactgct gttggtttgt taccaatcgc tgttagtggt gcagatatcg atgcattgat
    15721 gcaaggtgcc gctgatgcaa gcaaagcata ctcaagcgac aaactagaag aaaacgaagc
    15781 ttaccaatac gcagcaatgc gcaacattct ttaccgtaaa ggcaaagtaa ctgagttgtt
    15841 gatcaactat gaaccaggaa tgcaatactt ctcagaatgg tggaaacaat tgtatggtga
    15901 atctgaagga aaagaccaaa aaggcatcta tccttcaagc gcaaacttct caactgattt
    15961 gcactcttta ggacaattca tccaagaagg acgtcgcaat atttttgaaa cagttgtgaa
    16021 ggttgaaaaa cctcgtaaat caatcacaat tcctgaacaa gcagaagatt tagacggtct
    16081 aggctacttg caaggaaaag aagtggactt cgtaaataca aaagcatttg aaggaacatt
    16141 gcttgctcat acagacggtg acgtgcctaa cttgctagtc aaaattccag aaatggatgc
    16201 ttatacattg ggttatacaa tgtacttctt tgaaattgct gtaggtattt caggttactt
    16261 gaatggaatc aacccattcg accaaccagg tgtagaagct tacaagaaaa acatgttcgc
    16321 acttctaggc aaaccaggct ttgaagatct tgctaaagaa ttgaacgaac gcctgtaaga
    16381 taagggtttt tcgctagatt taaataatca gtgatagaaa catgttcgct ttaaaatgaa
    16441 aactggtacc catttaggta ctcaaacata ctgcatcgtt tattcagaat aaggggctgt
    16501 gacaagacgt ttgtcacagt cccttgtttg tatagaaatc gattgattgc cttgctcttt
    16561 agtttgtttt aggactttaa gaacagtgag gctgacttcg aaggttaatt ctgttttttt
    16621 gttttctaga atcagtaagc aaaaaagcac tcacttcgag tgcttttttg cgatatattc
    16681 ttagcttaag ataggcctaa tttttctttt acagatgagg gtaccttatc gctggtaatt
    16741 acttttttct ctatgatccg cttttcgctt gctttcaact gcaagatcgt tccttcgggg
    16801 taagcagaac cattggcaga gtcaccagaa gtaatgacat cgaaatctaa ctgtcttttt
    16861 tcaccgtttt cagcataagc atctacgttg tatttaaaac ctcttccaat tacttcccca
    16921 ttatctgctt tgatatcagt ttctgcaggc attggagctt tgactaccgc ataataatct
    16981 ttacctacat aagtagactg atagtagtcc caacctttcc atcctataaa agcaatcaat
    17041 atgataacga tcaaccctaa aatttttttc atgaaagatc tcctttttta tttt
//
LOCUS       DACDSY010000058        16922 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDSY010000058 DACDSY010000000
VERSION     DACDSY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.058"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..968)
                     /locus_tag="IWK44_001527"
     CDS             complement(<1..968)
                     /locus_tag="IWK44_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9550061.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLK"
     gene            complement(959..1627)
                     /locus_tag="IWK44_001528"
     CDS             complement(959..1627)
                     /locus_tag="IWK44_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9550062.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(2083..3069)
                     /gene="lacD"
                     /locus_tag="IWK44_001529"
     CDS             complement(2083..3069)
                     /gene="lacD"
                     /locus_tag="IWK44_001529"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9550063.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(3082..4008)
                     /gene="lacC"
                     /locus_tag="IWK44_001530"
     CDS             complement(3082..4008)
                     /gene="lacC"
                     /locus_tag="IWK44_001530"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP9550064.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(4021..4536)
                     /gene="lacB"
                     /locus_tag="IWK44_001531"
     CDS             complement(4021..4536)
                     /gene="lacB"
                     /locus_tag="IWK44_001531"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP9550065.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(4555..4983)
                     /gene="lacA"
                     /locus_tag="IWK44_001532"
     CDS             complement(4555..4983)
                     /gene="lacA"
                     /locus_tag="IWK44_001532"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP9550066.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(5003..5776)
                     /locus_tag="IWK44_001533"
     CDS             complement(5003..5776)
                     /locus_tag="IWK44_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9550067.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            5941..6420
                     /locus_tag="IWK44_001534"
     CDS             5941..6420
                     /locus_tag="IWK44_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9550068.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            6477..6779
                     /locus_tag="IWK44_001535"
     CDS             6477..6779
                     /locus_tag="IWK44_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP9550069.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            6801..8258
                     /locus_tag="IWK44_001536"
     CDS             6801..8258
                     /locus_tag="IWK44_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAP9550070.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(8851..9603)
                     /locus_tag="IWK44_001537"
     CDS             complement(8851..9603)
                     /locus_tag="IWK44_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9550071.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(9818..10054)
                     /locus_tag="IWK44_001538"
                     /pseudo
     CDS             complement(9818..10054)
                     /locus_tag="IWK44_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(10064..11022)
                     /locus_tag="IWK44_001539"
                     /pseudo
     CDS             complement(10064..11022)
                     /locus_tag="IWK44_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(11228..12910)
                     /locus_tag="IWK44_001540"
     CDS             complement(11228..12910)
                     /locus_tag="IWK44_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9550072.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            complement(12921..13244)
                     /locus_tag="IWK44_001541"
     CDS             complement(12921..13244)
                     /locus_tag="IWK44_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9550073.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            complement(13267..14673)
                     /gene="lacG"
                     /locus_tag="IWK44_001542"
     CDS             complement(13267..14673)
                     /gene="lacG"
                     /locus_tag="IWK44_001542"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9550074.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            15140..16147
                     /locus_tag="IWK44_001543"
     CDS             15140..16147
                     /locus_tag="IWK44_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9550075.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            complement(16541..>16922)
                     /locus_tag="IWK44_001544"
     CDS             complement(16541..>16922)
                     /locus_tag="IWK44_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HAP9550076.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
BASE COUNT         5071 a         3600 c         2781 g         5470 t
ORIGIN      
        1 tgcttgagcc catgcactcg gtactgatga gaaaaaattg atgcctgttt ctcctgagga
       61 atgccaattc cctgatcttt tacttcaata atcacgctac ccttttggtt tgaaatggtg
      121 acaattacca gttttccttc accatatttg attgcattgt caactaaatt cgaaaccgcc
      181 cgataaatcc aacgatctcg tccatgaatc agggttggtt gatcttcagt aaacgcaaac
      241 aaaagattcg gataattcat tttgtaggca tcacaaactt ccgcacagat cagtgaaacg
      301 tctacgactt ccgtgctaag atcctctgag ttattggata atgtcaaaat atcgtcaata
      361 ctgtcggtca tccgatctaa aagcattaaa tttccttgat cggcatgttg ccgttctatt
      421 tccagattcg tacgcaaaat agcgatggca tttttctgct cgtgggtcag ataactattt
      481 aatcgtcgat aatcctccaa atgaccatcg aacttatcct ttagcttatt aaaagccgca
      541 tttaatgcct cgtccttcac agtattttta ggaacttgat caataaacgt taaatcacgc
      601 gtaatcgcta agatttgatt actgtaaatt ttgcgtaaaa ccaaccaaag aataaacgtt
      661 aataacatga cggcagaaca aataaccagc accatcgctg gtaaatattt aaatagttcg
      721 tcctggtaag ctttatctat catcgaaata gtcataaatt gattgttgga agatttcgaa
      781 gcttccatct gcgtctctgc ttgaaccgtc ttaccctctt ccataaaata aacatcttca
      841 cctaaggtag atgcgatact gattccctcc aaattatttc tcagaaagaa gaaactcact
      901 aaaatcacgc ctgtcaccaa cagtgaaaaa gccagcatcg ttaacgtcat tttaaaattt
      961 aatagcatag ctggtacccc tttgctcgaa tggtttttaa gacctctttt tcagctgctt
     1021 cagccagttt cttttttaga cgagccagat gcactcgcag aaccgacgaa aaaggatcaa
     1081 aatcttcatc gtagacatgt tcagaaattt cctcagacga aacaaccgct ggatgacgct
     1141 gagcgatgca cagtaagata tcaaattcct tgggtttcag gaccacctct gtcttttgat
     1201 agctgaccct tcgacctaat ggatcaatct tcaaagcacc aattgaaatc tgcggcagcg
     1261 cacgtccatg ataacgacga ataaccgcgc gtattcgtgc ttttaattct agtaactcaa
     1321 agggtttgac taaataatca tctgctccaa aatccaagcc ttttgctcgt tcttcaatct
     1381 catcacgtgc agtaataatg ataactggac tttcgacttc ttcagcacgg agactttgta
     1441 aaacagataa tccatctttt ttaggcagat ttaaatctag tagtatccca tcgtatgaat
     1501 tgatgaaggc cttctcttct cctgactcgc catcataagc tacatctaca gaaatattct
     1561 cctgctctag gccctttttt acggataaaa ccagctcacg attgtcctca accaccagta
     1621 cacgcatgta tattcactcc tcatacggat ttagtaatta cacttatgct ttttagcata
     1681 gtacacgctc ttcttcaaag tccacgttct ttttgtgaag atttgaagaa attcatacat
     1741 tctgttaaaa aacacataat taagaatgat tttactgttt gaaaatacct agaatagaaa
     1801 aagactggaa cctttcctcc agtctcacta ctttctattt gcttggtttt cttgtatata
     1861 cttttcaaag ttcgttggtt tatgaccaat aagcctcgtt agaatactga aaattgccat
     1921 caagtcatta agtaccacat gtaagaaaat ctctctaaag aaaaacccct tcgttttaac
     1981 tcacttacta ttttagaatt cgttccccta ataatgcagc atgattttac gctttatgta
     2041 taaaaaaaga ctgcaagcaa ttttgcttca cagtctgatt tcttatgata gtgagcttgt
     2101 taacacttcc accggtgtag cagtttcttt caatacggca tttaattctt caatattttt
     2161 acgcccttgc gtattcatcc actccatcgc tgcttctttt ccatcaacga tatagctttc
     2221 tacaccatct gcccaagtcg cacggccgca aagtacacca ttgaaggttg agccggcatt
     2281 tttcgcgaag cgcagtgttt cttggaacaa ctctgctttt acaccggcgc ttaggaaaat
     2341 aaatggcagg ttagtggctt cagcctgttc cttgaagaag ttagccgctt gtgcctttgt
     2401 gtacacgact tcgtctgtcc catagccttc cacataattc atattgacgg gtacttccac
     2461 ctttaacaca tctacgccat aacgtggatc agaaaattcc ttcatcatat cgttgacttt
     2521 atgaggtttc ttaatggcgt attctttcga agtcgcatca gagatgttcg cgtcataaga
     2581 aacaagctcc aagaagaagg gtaagttttc agcctgacat tctgagccga ttcgttcaat
     2641 aaaggcatgt ttttgattat tgatttcttc tgcttcatcg acatcatagt aaagtaggaa
     2701 cttacaagca tcggcgcctg cttcttttaa acgtttaact gaccagatcg ataataaatc
     2761 tggtaaacgg ccgggagttg acgcatcgta accagttttt tcataagcta acagcaagcc
     2821 ggcatttttt gctctaacgt cagctgctgg caagccatat tcgggatcta ataaaatcga
     2881 tgaggcatat ttcgttaatt gtgaagaaac aatttcttta aattcaacga tttctgattc
     2941 ttctgcttcg ctgcccttga attttgtgat catcttcttc aaagccccgc gttgatcaat
     3001 tgctaaggcc ccaatcactc catcttcatt gcatagttgc ttgatatatt gacgtttttc
     3061 ttcgttcatt tccgtatcct cctacacttt ctccactaag atttgatcat atagtttttg
     3121 ataattggct aattggacat agccagtcgt cgcttcctga gcattcagca ttcctaaaac
     3181 attaccctgt tttaacacgg cttcatccga aagaccttgt tgaatcgcag cggtcaatcc
     3241 ggcaacggtc gcatcaccag agccaactgg attgacgaca tcaatctttg gaattgtcac
     3301 gcaataaaaa gtctcgtgat gtttggcaaa ggctccgtcg ccccccattg aaaccactat
     3361 ccattcaatt tcatcaaaca tcgaatctga caaggcggct tttaaatctg ataattctgt
     3421 tgagatttct cgtcctagca gaccagccag ctcttcggta ttgggtttta ttagcttagg
     3481 ctttacactg cctcgcaaca cttcccttaa tgctgtacct gaacaatcta aaacgacagg
     3541 ctttggctga caaacttgca gcatttgtcg ataaaaatca gcaggcaatc cttctggcaa
     3601 gcttcctgaa aaagaaatga tttcggcttt aacagctaat ttttcgaaat gttgcaagaa
     3661 cgcccttgct tcttgttcag taataacagg tccgttttct aggatttccg tctgttttcc
     3721 gtcatgtaaa acagcgatgc aattacgtgt ctctccgctg ataggtaaaa agtcatgagc
     3781 aatctcattt tgagtcaggt cctttttaat ctcttcacct aaaaaaccgc cgattaaacc
     3841 actagcaatc acctccgtat ctaattgctt caagatacgt gtcacattta ggcccttacc
     3901 gccagctgtt ttgcgaacat ttcttactcg attgactgtg tcgagcttga aggtttctaa
     3961 tggataagaa atatcaatcg atggattcat cgttattgtt aaaatcattt gtccacccgc
     4021 ttaatcgtgg tattcgccac gatcccattt ctcgataaat tcatcgaaga aatgcggatc
     4081 agactgcttg tcactggctt tttcgacgtg atcgattttg gcgatcaatt ttttgttttc
     4141 ttcagtttct tgataatcgg cattgatgaa ggcctctaat atgtcacaga tcaaaaattc
     4201 tcccgtaatc cgagcgccaa agccgatcac attggcattt agttgttcct tcgcatagat
     4261 agcagtcgtc atatcgcgaa ccagcgcact gcggatacca ggtactttat tgacagcgtt
     4321 gttaatccca acacctgttc cacacaaaca aactcctaga tctgcttctc cgctagtcac
     4381 ggcctctccg actttttttc cgtagatcgg ataatgggtt ctggtaaagt cataggtccc
     4441 catatccaac acttcatgtc cttttgattt taagaaatct gatactgcca tttttgtatc
     4501 tgtgacgatg tggtcacagc caattgcgat tttcatttca ttcatccttc ctaattacgc
     4561 cattttattt aacatgtcga cacgtacttg atgacgacca ccatcataat ttgcttgtag
     4621 aaattcctta acaatattca acgccaactc atcgccaaca atttttgatc caagtgtgat
     4681 catccgagaa ttattatgtt tacgggtcat atacgcactg cgctcatccg aaacttctgc
     4741 tgctaccatt cctttaacct ttgttgccgt gataaagctg ccagcgccgt agccatcaac
     4801 cgcgattcct aaactacctt cgttttcatt cagatcattt gcgatcgcca aagttgattc
     4861 cacaaaatct tctgaagccg tttcactctt atctactact gtaatacctt ctttgatcaa
     4921 ttttttcttg attaattcct ttaattccat gccgtcactg tctgaaccaa ttactacacg
     4981 catttttgtt tcctcctatt ttttactgaa tgattttagt gaattgctga taataactct
     5041 tcagctcggc tgaaagagta gaatccgtaa ataatttgtc gatcttgcgc aaatcataga
     5101 aattataaaa atcgctatgg tcgaatttgt ttgcatctga aaccaaacat ttttctgccg
     5161 cattatctaa aacaattttt tgcaaggtac cttcttcaat actgaaattc gaaacctccg
     5221 aacccttcaa tccattcact ccgacaaaag ccttcttaat accgattttt tgaacagctt
     5281 cattggcaat tgaaccgaca aaggctccag tcttttcccg atatagccca ccgatcaaat
     5341 acagttcaaa tctttttatt tcacttaata gattgaaaac cggcagacta ttggtaacga
     5401 ttcggagaga tgacggcttg atgtatcgtg tcattaattc gatcgttgta cccgatccaa
     5461 gaaaaatcgt gtcgtcttct tgattgatat gagctgccgc atttttagcg atgtattcct
     5521 tctcttttgt atgcagcttc tttttttcag tatgagaaag ctcatttgct tctatagaag
     5581 tcgacatttc cagataatcg attttttgtg caccgccatg aacgcgtaaa agctttttct
     5641 tctgcgcgag ttcctctaga tctcggcgga tcgtcatatc tgaaacattc agtacttcct
     5701 gaataaattt gactgttatc gtcccatagc gatcacacat cgctaagatc gaacgctgtc
     5761 gttctgtttt cagcatattc tgtacctcca cgaccttata ataaaacatt tacgaacatt
     5821 tttcaacaca tattgttcgt ttattttcgt ttatgcgttg acaatgattt tgtaagcggt
     5881 tatactcaac ttgtaaacat ataaataaac ataaacgaac taataaagga gctgttttgt
     5941 gtgaatgaat tctttttcaa agacactgtc tatgtttctg acaagcagtc gcaggaagaa
     6001 gtgttcgagg aagtctatca agatctgtta aagaaagaat tggtaaccga agattttttg
     6061 gagaaccttt tagaacggga gcgtaattat ccgacgggat tagatctctc acctgtctct
     6121 aaaatattgc cagatatcgc tatccctcat accgaaagtg agtttgtgcg aaccacccgt
     6181 atcattccaa tcaaagtaaa aaatgaaatc cctttctaca acatgatctc gccaagcgat
     6241 cagatttccg cttcatttct tttcatgatc ttgaacgaaa atggtgaagc acaggctggc
     6301 atgttggcta atatcatgga tttcattaat tctgttgacg aagaaaaact gttttcgttt
     6361 ttcaaattag aggacacaga agcgatttat cattttttag aagaacattt ttatatatag
     6421 aagtctctgc ctaaccgcag gataaaaaat aaacttattt taaggggaga attattatga
     6481 tcaaaatttt agcagcatgt ggagcaggcg ttaattcaag ccatcaaatc aaaagcgctt
     6541 tggaaactga attgaaaaat cgtggctatc aagtcacctg tgacgccgtt atgatcaaag
     6601 atatcaatga agaaatgctg tctcattatg acattttcgc acaaattgcc aacaccgact
     6661 tgggttttga cattaacatc cctgtcgttg atgcaggtgc tattctttat cgtattcctg
     6721 ctatggctga gcctgtttac caaagtatgg aaagcgcaat ccaatcatta aacaaatagg
     6781 actaaagaaa ggaaaagcat atgaatacaa ttattgatat tgccaataaa gtatttaccc
     6841 ccctaattaa tttaggtgcg gcaccgttaa tgaccatcgt attaactgtt attgctttat
     6901 tttttaaagt aaaaccgtca cgcgctcttg aaggcgggct caaattaggg atcgccctga
     6961 ctgggatcgg agctattatt ggaattttaa ctactgcttt ttcagacgct atgacagctt
     7021 ttgttgaacg cacaggtatt tcattaaaca ttacagatgt cggttgggcc ccactagcaa
     7081 ccatcacatg ggggtcgcca tacacgcttt attttttact aattatggtg atcgtcaata
     7141 ttgtcatgtt agcaatgaaa aaaaccaaca cattagatgt ggatattttc gatatttggc
     7201 atttagcaat tgttggtttg ttcgcgatct tctgcggtgc taatctgttt attgcttctg
     7261 cattagttat tttcattggt gtattaaaaa tttggaactc cgacttaatg aaaccaacct
     7321 tcaatgactt gttaaatgcg ccagacagca acccgatgac cacgactcat atgaactaca
     7381 tgatgaatcc gattattatg gttttcgata aaattttcga caaaatcttc ccttggttgg
     7441 ataaatatga tttcgatgct gccaaattga atagtaaaat tggcttctgg ggatcaaaat
     7501 tcgcgattgg aatttatttg gggatttttg ttggattact agcaggacaa acaccaacgc
     7561 aaattttctc gctagccttt accgctgccg tctgtttgga attgttctct ttgataggcg
     7621 cttggttcat cgcagccgta gaaccgttat cacaagggat taccgatttt gccaataaac
     7681 gactaaaagg acgtaccatc aacataggtt tagactggcc tttcatagca ggccgcgctg
     7741 aaatctgggc tgccgctaac gtattagcac caatcatgtt gctagaagcc atcatcttac
     7801 caggaaataa attattgcca ctaggcggaa tcatcgcaat gggtgttacc ccagcactat
     7861 tggtcgtaac tcgtggaaaa ttaatccgta tgatcgtaat cggcgcgatc gaattaccat
     7921 tattcttatg gtccggcacc ttgatcgccc cgttcgttac tcaaacagct aaagcagtcg
     7981 gagcctttcc agccggcttg aacgggtccg ccatgatctc tcatacaacg atggaaggcc
     8041 cgattgaaaa attcctaggt tatttagtcg gtaatgcttc tcaaggacaa atcgaatttg
     8101 tgatatacgc tgccttagca ttaggtgcat accttttaat cttcatttgg tacgctcgtc
     8161 aaatgaaaaa acgcaacgct gcatacgcag ctgaaaaaga agagaatgca tctccggcaa
     8221 cggcgcataa tatgacttat gcagccgaag ctaaataagc taaaaacacc ccgaatgtct
     8281 ttagacttcg gggtattatt ttacctataa aaaataaata gatgtaaaat ccatttcaaa
     8341 aagcatagcc catctcttgc cacacccaat aaagaaatta tcaacgttta tgcttttttt
     8401 aactccttta agcttgcctt tgttggtatt cctcaacgat tctttttgtg ttcttgtttg
     8461 ttttgtgctt tttgactttt tgttgtttaa ttgcgaattc cttgatttct tttttcctga
     8521 atcgcttcgc atcccgcggt tatgcgtgcg atttcatcat tgctttttct gatctaactc
     8581 gtttcatgtc aaagagcttc agctgactta gtgtccatgg ttgttttgat atcccttttc
     8641 ctccttaata aagcggtgat tgatactgct attttttcct tcacataaac ataacatccc
     8701 aaaaaaatcg actatctttt ttcaaatggt gtattcaagt attcggagga attagtaatg
     8761 atgcacccta aaaataacat aattcgctta aattcaaggc taccaaaaaa ctaccaagtt
     8821 ctaattatct agagtttggc agtttctcat ttacacaaca tatttttctt ctttttcgat
     8881 tttttcatag tgcttttgtt catcatcatc cgttataact actgttacat cattcagatt
     8941 ataatatact gaaaaatctc ttttcccaat tttcgaatga tcaatcaata aaaagcgttc
     9001 aacagaatta ttcagagcga tctgttgagt ttggccctct tcaaaagtcg aagtcataat
     9061 tttctgttca tttaaagcat tacagctgaa aaatgcttta tgaaaattca tatcctgtag
     9121 cgatttattc gtgatttcac caacaaagca ttgagtcgtt tcccgcatct cgccacctaa
     9181 tagatagatt tttcgattcc cttttcgctt atttaattct tcaaaaacag gcaaacagtt
     9241 tgtaacaatc cgcagtgaat ccccttgaat gatttttgac atgagctcga ttgtagttcc
     9301 tggtccaaga aatatcgtat caccatcatt gatcatttca ttcgcctttt ccacaatccg
     9361 ctgcttttct tcttgcagca taatctgttt ttctctatga gaaagctcca catgccgata
     9421 aaaatccttc aatttcgctc cgccatggac gcgttctaat aatccttgtt tctccatttc
     9481 gtccaaatca cgtcggactg tcatatccga aaccgaaaac ttctccataa tatcgctgac
     9541 tcgtactgta ccgttaacct ctaataacga catgatcgct tggaatcgat ccgtttttaa
     9601 catttcatcg cctcttttct ttttaagact atcaaattct taagaaaagt gctagaacaa
     9661 gttttcttaa gaagccttct gcaggaagga aagcaaaatt tttcagaatt tgcaagctgc
     9721 tattctaaaa aataaaaccg aacgagcatg actgtcatag tgacagcctt gttcattcga
     9781 cttttatctt taataaatca ttttatttaa tctgaagtta ttgtgcaagt tcaaaaacaa
     9841 agtagttaat gtctcctcga tatttcgaaa tcgaatactc aacgggcaga cgttctagcg
     9901 aaaaaccctg tgtatggaaa tagaacattg gatctccttc ttccacatct aacaaatcgc
     9961 tgatcgtatc atccgctttg attacttcta gttttctggt gaccgtttta attggataac
    10021 ccatttcttc aaaaacttga tacatttttt cttttctgat ttgtcaaatt aagctagaca
    10081 atatgtcttt atctatgcag ctcaaaaata cttccaatgg tgttcgataa tttaatgatt
    10141 ttcttggaat atggtttctt ttcgaggcaa cggattgaat aaaggtttca tccatgtccc
    10201 taaagtccat tgttttaggt aagccatctc gtctaagcaa gccgtttgag tgctcattta
    10261 gccctctttg cgaaggtgtt cctggatctg cgaaaaagat ggcaatatcg ttcgagttac
    10321 ttatagatcg ccagttggaa aattcttttc cacaatcaaa tgtcatggat ttaaacaagt
    10381 gtttaggcat ggatttgaac caatcattta acctgttttc gatatcttcg gctcgtctgc
    10441 ctgtcggttt caaagtaata ataaccttgg ataaacactc aaccaatgta ataacagcgc
    10501 tcttgtgttt aactcccaca atggtgtcgc cttcgagatg accaaattct ttagtcaggt
    10561 gtggatagag actgttccgt tcatgaatcg ttctgcgaaa ggcctgtttt ccacgctttt
    10621 cttgatgatt attcggttta cgtttcccct tcataggtaa cgactgtgtg tcaaaaaggc
    10681 cttttttaaa catccgatag agtgtacgaa cagaacaatc aattggaaac tcgccacggc
    10741 cgataatcac atcgggtgac cacccttgag ctaccttttc ttgaacatat gttgattgtt
    10801 catcagataa aacggttgaa cgtcgtccac aacgagattt attttgtttg tattgacggt
    10861 aataatctag cacttttttc cttgtttgag aaacgaataa acattataaa tggtttgtct
    10921 agagcgattt aaactttgtg cgacaagagc gacaggtttc tcgatattga aataagactc
    10981 tatgattact agttcgtctg ttgtaagatg ggtgtaggtc atttgtactc actccttatt
    11041 aattctttgg tcggaactat tttgagtgta gcacaaatga tctttttagt tgtctagctt
    11101 aattttacaa tcgacgcctc aaaaagaata cccgaaattt aaacagcaaa aaaagatagc
    11161 gcttaccatt gtgtttaaga taaaacgcta gctaagttgg ctagcgcttt attaatctaa
    11221 gcaagctcta aaattgcttc aatacaaaat caactgctcc tttaggatca cgtgttaaat
    11281 tgatatattc tgcaccttta gttgcaatta cttttatccc taatttgtct gcatctttac
    11341 ggagatcatc caaataggaa ttcacttgag gtgctagaac gattaaatca aaatttttca
    11401 tcatatcata atgagaacca taagctcctg ctttagcaga aagattaaca cctgtctctt
    11461 ttgcgccctc ttctaatgca ttagccaaca ttgcactcgt tccggcacca gcacaaagta
    11521 ctaaaacgcg ctttccttca ccaaccgatg cttttgcttc atcaactata actgccgcag
    11581 ttgcaccaac ttcttggatt tcttcgacca attcttcttt tacttcttct gcagcaatgt
    11641 tttttaactc ttgttctact aattcagaat cataggcctt aacaaaaggt aaataaataa
    11701 tcgcatcgac aacaatcaat actaccgcta atacaatcgc caacacacta aacccggttc
    11761 ctaaaaccaa gccaatcggt gcgggcgtcg cccaaggcaa tacggatata aagctattca
    11821 tccccaagac atcgacaaaa agtttgaata tccatacgtt agcgattggt gctaataaaa
    11881 atggaattag aaagtacgga ttcaaaataa taggagtagc aaatagtaac ggttcattta
    11941 ccgcaaacga aactggaata aatgaagctt taccaactgc tttcaattgt tttgatttag
    12001 cgaacaacat gaacagaaaa ggaacaacta gtgtcgctcc agtaccaccc atcgttccaa
    12061 caaagtttcc tagaccaact gttaatactt ttgaggcttg tgcaccgttt ttaaataatt
    12121 caaagttagt ttctacgttc gcataaataa ttgcagcaat cgctggttca acaatcgatg
    12181 gtccatgaac accaacaaac cagaacattg ccatggctcc ccaaatgatt gcaataccga
    12241 catatccatc agcagcacta aataatggct gcaataattg aattacacct gctgcaaatg
    12301 atgttccaaa aaattgacga actcccgtat caatcaaaac ggcaataaat actgcaattg
    12361 aaaaggggat aatatctcta aacatctgtg tgattgttcc gggaacttct tttggcatac
    12421 ggatcgtcaa atctctttta acacagaatt tatacacatt tacagtaaca aacgcagaaa
    12481 cgaaagctgc taataatccc tttgtcccta aataatcagt agctatccca ccatcaattt
    12541 gatcagctgc catcattaca aaaccacaaa ttgacgctaa cataacagaa acttcattaa
    12601 tctgcttacc tgaatcagat aattgacggt tcattgaatt agctaaacat cgagctgttg
    12661 tagcaacaac caaaagacca actaatccca ttgaataacc ataaactttc caaagccacg
    12721 tcgagaatgc ctctggtaac gtcacgttaa atacttctgg aacagccgca atcataataa
    12781 aaatacttga aaatagaata gctggcatag cagttaaaaa gccatcgcga attgccccta
    12841 aataaatatt acgtgagatt ttttcaaaaa acggttttcc cttctcaatt tgcttaataa
    12901 tcgtattcat ttttcttctc ctatgcttta gcaaattttt ctttgtacaa ttcaacaaaa
    12961 tcattgataa gttctcttaa tagaatcgtt gtcattaaat ggtcttgccc atgaataaaa
    13021 attacgccta cttccatttc gttccctgac gcttcttcag ctaaacattt cgtttgtgag
    13081 ttatgcgcta aggttaaatt ttcatcagca tccgctaata atcgttcaac attatcaaaa
    13141 ttaccttctc tcacttcctt caacactgtt aataatgaac ttctggcatc tcctgcataa
    13201 gcaacaattt caaatcctaa taattgcaat tctttcctat ccatttcctt gccctcctaa
    13261 ctatttttat attatcgttt ttgtttcgct aacaaattta taccaatatg cagactcttt
    13321 cggatatctt ttttgtgtat cgaaatctac atagaatagt ccataacgct tagtgtatcc
    13381 attaacccaa gagaaaacat ccattaatga ccaaatgaaa taccccttta cattaactcc
    13441 cattttgata gcatcggaaa tcgcattaaa atgctggcgt aaataatcga ttcgatcatc
    13501 atctagaata atcccatctt caaaattatc cttataccct agcccgtttt cagtaatgta
    13561 tattttatta taatttggat attcgtcttg gattcgtaca agtaaatcat ataacccttt
    13621 tggataaatg atccaatccc aatcggtttt cggaagatcc tcacgcacga ttctctcgcc
    13681 aatcccttta atgcaataga cttggctacc tttttctccg gtagtgttgt agtgaagtgt
    13741 gctttctcct ttgtactctt taacccaatg gctaccatag ttattgatac caagaaaatc
    13801 atttcgattt gctgcttttt tcatctctct aaaatcatta gaatcaatat ctgcagattg
    13861 cccatttgct gctaaaatct cattaatatg tgccattgtt tcatctgagt aataccctaa
    13921 ataagtagca tctaataaga acttaattga taacgcatca tctaatttcg cagcatgctt
    13981 atcttcttct ttgtcacttg ctggatattt tgtctctagc gagtgaacaa caccaatttc
    14041 ccctttatat ccaccatctt taaaaatgtt tactgtcttt gcgtgcgcat acatcatgtt
    14101 gtgtaaacaa gttacagtct tctctatatc aaattgaact tctggaggaa acgatccgat
    14161 caaatattga tttgaagcaa ctggataaat ctcattaaat gtactccaat aatgaacctc
    14221 agaaaattca tcgaaacaga actgtgcata gtttacgaaa tgttcaacat tttctcgatt
    14281 taaaaaatct ccattatcaa atagttcttt aggggtatca aaatgatgaa gagttacaaa
    14341 aggaataata ccatgcttat tcaattcagc aaatacctta tgataatatt ccactcctgt
    14401 ttggttcact tccccatagc cttttggaaa aatacggctc catgcaatag acatcctaat
    14461 actatcaatt ttaaattttt tgcataattc agcatcaact ggatattgat tataaaaatc
    14521 actagctgga tcagcagtat agcgtccctt ttctacaagg aaagtatccc acgctacttg
    14581 cccttttcca ccttcttttg tagagccttc tgcttgataa gctgccgtag ctcctcccat
    14641 aataaagtct tctggtaaag ttttataatt cattttaatc tctccttgaa aattagtttg
    14701 atcaatatgc caataatcca taaatcacct cagcataatt ttaacatgta taagtaaatt
    14761 tttttacaca acgaaaaacc gacctcgaat atctatttta ttgcgatata caagcactta
    14821 tatcccttga tgtaaaataa atataccatt caaaaaccgt tttgtaaacg tttttttgta
    14881 aaataaattt atctttttac taatttaatt tacacgaaac aagaaaacgg actgattccc
    14941 actaaaatca atcccaccta ctaatcattc tccaagtggc tatccattat cgattcaatc
    15001 ttctctgtta atggattagg ctctctgctg atatcagagt ttcgtttcct gctagaaaaa
    15061 gtaatttaag ttactaaaag agcagtttcg atttacttta acaaagaaaa ggattattat
    15121 atccctagga ggatgaacaa tggttactat tagagatatt gcaaaaaaat ctggttactc
    15181 aacggctact atatcaagac tttttaaagg tgatgagtca ctctctatta catctgatac
    15241 taaaaacaaa ataatcgtta cagccaaatc tttaggatat gatctgacaa aaattaagac
    15301 tactctgtac aagattgcca ttcttttttg gatctcagag gaacagttaa aaaaggatgt
    15361 ctactatcaa catgtcaaag atgatttgaa aaattatagt aaaaaagcta atctagattt
    15421 gattttcatc tctaaaaacg atggtgtaaa aagcattcct gacgatatta ctggatttat
    15481 cgcaatagga caattgacaa aaaaggaatt gtactactta aatcaaaaag ggtataaggg
    15541 ggtaatccga ggaataaacc cattacctga acacttcgat actgtagaac ctgatttcca
    15601 cgaaatggcc agacaagcaa ttaactactt tattaatgaa ggtttcacaa aaattggatt
    15661 tattggagga aatttcttta acctagaaac gggtaaagaa gagattgata gccgtgaata
    15721 tgccttccga tatcatttag aaaagagcgg actactcaat gaaaagtata ttttctccca
    15781 aggatcattt actgttgacg aagggtatca attaactctt aaaatgaaag ctgagttaaa
    15841 tgaagatatg cccgaggcat gtttcatacc atctgacact atagctgccg gcgttcttca
    15901 gggctgccat gaattgggta ttaacatccc taaagatatg gcagtaataa gtattaatga
    15961 tgacgacatc gcaaaattcg ttgcaccacc acttacaact tttaagatag atatcgaaga
    16021 gatagctaaa acctgtgtag atttattaag tgatcaactg gtatattcgc gggacatatc
    16081 aaagaaaaca ttaatcggcg caaaattgac cgttaggaaa agttttatcc ctaaagaaga
    16141 ggtgtaaaat aagtaattga aacttatctt taaaactata attgtgtcca catagtagcc
    16201 gagcaatcgg gtttatgcct attatgtttt ccataaataa tttgatggtg atattttgac
    16261 gtaaatacaa tatgattttt tcatcgccaa attgtgcgcg ctaatttttg tttatcattc
    16321 agaacatttc ctccttttta tctaagtatg aggttggata aacgcttcat cttagtatct
    16381 tgggggattt tttatctcgt tgggaatata tcaagcatca gagcgcgcaa aaagaactgg
    16441 tcgaaaaatc aatctaccag ttcaatctca ttcactgtga tactatgttt tatacagata
    16501 acgccatatt ataaaacaag ttgtattccg caaatgggtt ctattcaact cttaaactat
    16561 ctataaaaga ttcagccaac tccattagcc tctcgtaatt tagttggtga acagcacttt
    16621 tttcaaaaaa ttttgaacca attcctacat aagaggctcc attgttcaaa tagttttgtg
    16681 cattagtctg atctacccct cctacagcca tcaaaggtaa acttcctaaa ggggcttgca
    16741 cttgattgaa atattccggt ccaagacagt tggcggggaa caccttaaca atatcagcac
    16801 ccatctcaaa cattttgaat atctctgtgg gcgacatggc cccaggaatt gttagaaggt
    16861 tctgttgcga agtttgaaaa tcaaacgcgc cctctctgaa ctccaaatat cttaggctgt
    16921 ta
//
LOCUS       DACDSY010000059        16820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDSY010000059 DACDSY010000000
VERSION     DACDSY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.059"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1001..2251
                     /locus_tag="IWK44_001545"
     CDS             1001..2251
                     /locus_tag="IWK44_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP9550077.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            2270..3280
                     /locus_tag="IWK44_001546"
     CDS             2270..3280
                     /locus_tag="IWK44_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAP9550078.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISTERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFENGGTRVIHYTPETYIEKQ
                     NG"
     gene            3290..3943
                     /locus_tag="IWK44_001547"
     CDS             3290..3943
                     /locus_tag="IWK44_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9550079.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            3962..4960
                     /locus_tag="IWK44_001548"
     CDS             3962..4960
                     /locus_tag="IWK44_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9550080.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWEPTVSLEEGLRESYNWIEKQIKG"
     gene            5036..5797
                     /locus_tag="IWK44_001549"
     CDS             5036..5797
                     /locus_tag="IWK44_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAP9550081.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            5815..6921
                     /locus_tag="IWK44_001550"
     CDS             5815..6921
                     /locus_tag="IWK44_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9550082.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRNVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            6911..7144
                     /locus_tag="IWK44_001551"
     CDS             6911..7144
                     /locus_tag="IWK44_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550083.1"
                     /translation="MINDMEVTKLMALRNRYALNIVDNCTRKIAKILGCCIGKGAQIG
                     NSVEFVHNSVGTVIHSDTILEDGVKVYQNVTCG"
     gene            7343..7798
                     /locus_tag="IWK44_001552"
     CDS             7343..7798
                     /locus_tag="IWK44_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAP9550084.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            7812..8585
                     /locus_tag="IWK44_001553"
     CDS             7812..8585
                     /locus_tag="IWK44_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9550085.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            8572..9702
                     /locus_tag="IWK44_001554"
     CDS             8572..9702
                     /locus_tag="IWK44_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9550086.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            9733..10968
                     /locus_tag="IWK44_001555"
     CDS             9733..10968
                     /locus_tag="IWK44_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550087.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFIPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLNVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            10993..12102
                     /locus_tag="IWK44_001556"
     CDS             10993..12102
                     /locus_tag="IWK44_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550088.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAEYEKYMDFVWEE"
     gene            12144..13478
                     /gene="gmhB"
                     /locus_tag="IWK44_001557"
     CDS             12144..13478
                     /gene="gmhB"
                     /locus_tag="IWK44_001557"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAP9550089.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            13513..15149
                     /locus_tag="IWK44_001558"
                     /pseudo
     CDS             13513..15149
                     /locus_tag="IWK44_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
     gene            15251..15646
                     /locus_tag="IWK44_001559"
     CDS             15251..15646
                     /locus_tag="IWK44_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP9550090.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            15664..16395
                     /locus_tag="IWK44_001560"
     CDS             15664..16395
                     /locus_tag="IWK44_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550091.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            16419..>16820
                     /locus_tag="IWK44_001561"
     CDS             16419..>16820
                     /locus_tag="IWK44_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9550092.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFS"
BASE COUNT         5455 a         2373 c         3724 g         5268 t
ORIGIN      
        1 tctttgtacc accaatgatt tatatcagct caacagagtg aattgtattt acgcaagaat
       61 gcgtaggatg aaatggatag ataattaaga tatatatgta tatggtgact gaagacaaaa
      121 aaacagaact aatgaaactg aataaaaata tagagtgaac tagtttctaa gagggatttt
      181 aaactagatt tcattggtaa taaaatttgg acagatggaa aaacatcaca taaggaagca
      241 gattttgttg tcatttttgt tcctacaaac tatggtagtg aatgtgttat tgaattagtg
      301 atgtctgtta cttcagaggc attggggata attaaatcca ctatacctgt aggatataca
      361 aaaagtggaa gaaaaaaata ttatcaatag attttattta gttcagaatt tttacgtgaa
      421 agtagattat taatgataat ctttatccaa ttagaattat tatcgtagga tgtgatgatg
      481 aaagccgtga aattgttcaa gtatttacca atgtctccct gccagtaaat ccgcttagaa
      541 tttgagcctt gaaaatgtgc aatgataaaa aacaaaaact cgctcaaaat agacagtttg
      601 gggtgggaat agaccaataa tgtaccaaac aaacttccat ttggggtgaa aataggtcaa
      661 taaagcgatg ttaaacatac ctttggagta agaatagagg gtaataagtg aatttataaa
      721 ctttttggtg actggttgag tggaaaatgt aggtacattt caatgcgaaa atgtattata
      781 accccaaacg aggtcaataa aaatgactca aacttgcaat ataaccctaa acgggtaggg
      841 gatttatgaa aaattgaatt tgccggcgta ggagctggct gttttaagta tgaatgaaca
      901 cagctttcgg gctgttttat ataaatagca ggttactgtt cacaaacagc attttgttaa
      961 gaaagtagtg accacagcag tggtggaaag aggaaatatt atgaagaaac agtataacat
     1021 tgcagtcgcc ggcaccggtt atgttggttt gagtattgcc actctgttgg cacagcacca
     1081 taaggttaca gcggtggata tcattccaga gaaggttgaa ctgattaata accgtaaatc
     1141 tccaatccag gatgattata ttgaaaagta cttggctgag aaagaactga atctgaccgc
     1201 aacattggat gcaaaggcag cataccagtc tgcggatttt gttgttattg cggctcctac
     1261 taactacaat agccgcactc aacattttga cacatccgct gttgaggctg ttattaggct
     1321 ggtcatggaa gtaaatcctg atgccattat ggttattaag tccaccattc ctgttggata
     1381 caccgcttct gttcgtgtaa agactggtag caagaatatt attttcagcc ctgagttcct
     1441 gcgtgaaagt aaggcactgt atgacaatct gtatccttcg cgtattattg tgggtaccga
     1501 tatggatgat gagcgtttgg ttgaggatgc acacactttt gctacactgc tacaggaagg
     1561 cgcaatcaaa gagaatgtgg acacattgtt catgggcttt actgaagcag aagctgttaa
     1621 gctgtttgcc aatacatatc tggctcttcg tgtcagctac ttcaatgagc tggacactta
     1681 tgcagagatg aaaggcttga acactcagca gatcattaat ggagtttgcc ttgatcctcg
     1741 tattggtact cattacaaca acccttcctt tggttacggt ggctattgcc tgccgaagga
     1801 taccaagcag cttctggcca actacgcaga tgttcctgaa aatctgatcg aggcaatcgt
     1861 tgaatctaac cgtactcgta aggactttat tgcggatcgt gtacttcaga tggctggtta
     1921 ttacaattac gcagaagcaa atcagtatga tgctggcaaa gaaaagcctg ttgtgattgg
     1981 tgtttaccgt ttgaccatga aatccaattc tgataacttc cgccagagtt ctatccaggg
     2041 cgttatgaag cgtgtaaaag ctaaaggtgc aactgttgtg atttatgagc cgaccttgaa
     2101 agatggagag actttctttg gcagtaaggt tgtgaatgat ttggaacagt ttaaggcaat
     2161 gtgtgattct gttatagcaa atcgctacga tgcttgcctt gatgacatta aggaaaaagt
     2221 atatacccgt gatatcttcc agagagacta agattaggag agagtattta tgattattac
     2281 aaaaacaccg tttcgtatgt ctttttttgg cggtggaacg gatatggagg attatttcaa
     2341 agagaatggc ggtgcagttc tttctactac ttttgataag tactgttatg tgaatgtgag
     2401 acatctgcct agattttttg attacagcgc tgagttatct tattctcgta ctgagagagt
     2461 aactagcatt gaagatattg agcatcctgc gattcgtaat gcgatgaaaa tgctcgatat
     2521 gcatgaaatt cggcttacat acgaagctga tttacctgct cgctctggtc tgggtacatc
     2581 cagttctttt gcagttggta tgttgaatgc tttttatgca ctggagggta agtatgctga
     2641 caagaagaaa cttgctgatg aagccattta tcttgaaaga gttctgtgta atgaagccgg
     2701 tggatggcag gatcagattg ccgcatctta tggtgggttc aatcgaatta atttcaatgt
     2761 ggatggttat gaggtgctgc ctgtcattat ttcgactgaa cgtaaaaagc agcttaacaa
     2821 caatctgatg atgttcttta ccggattcac tagattttct tctgatgttc aaaaggctaa
     2881 caacgtcagt ggcacagagg ataagagagt aagactgaaa aagatgtatg agcttgtaga
     2941 tgaagctgag gcaattctta ctgataagaa cagaaattta gatgatttcg gtagacagct
     3001 tgatgttact tggagattga aaaaaggtac tggtggagca attagtacag gaagcattga
     3061 tgaactatat gaaaagggta tggctgcagg tgctttaggc ggaaagttgc tcggtgcagg
     3121 tggtggaggt ttcttggttt tctatgtaca acctgagaaa caggatgcag ttcgttgggc
     3181 tatgcgtgat ttgatgtata ttccgtttga atttgaaaat ggtggaacta gagttattca
     3241 ctacacgccg gaaacttata ttgaaaaaca gaatggataa ggagtaacaa tgttagcgga
     3301 aaaacttaat aaacatattg acttattgat taaacgatat cctacacttg agacgattaa
     3361 acaggacatt atcgatggat acctaattat ggaggaatgc tatttgaatg gtggtaaact
     3421 tcttgtggca ggaaacggag gatcagccgc tgactcggaa catattgctg gcgagttgat
     3481 gaagcggttt aagacgccac gtcctgtaag taaggaattt gccgataagt tagttgcaat
     3541 tgaccctgta agaggtccgg aattagccaa aaatctcgag tgtggtttaa cggcaattcc
     3601 tttggtcgca cacgaagcat tgtctactgc ttatattaat gatgtcgatg gtttgggtgt
     3661 cttcgcacag cagctgtttg gctttggtag atctggtgac gtattccttg gtatttcgac
     3721 gtccggaaat agcaagaata ttatgagcgc aactgttgtt gctagggctc tcggtataaa
     3781 agttattggc ttgacgggcg caaagggtgg ggaacttgcc acggtagcag atgtttgtgt
     3841 taaggctcct gaagttgaaa catacatgat tcaagagtat cacttgccga tatatcattg
     3901 ttggtgtttg atgctggaag ataaattctt tggaaacgaa taaaatttgg aggaatgtta
     3961 aatgaaaaag acaattatgg ttaccggtgg ttgcggtatg attggctcca atcttgttaa
     4021 gagactggtg aaggaaggct gctgggatgt ttatgtagct gataatctgt ggagaggaaa
     4081 gttggagtac ctgaacgacg aagatgggca tcctgtcatc gacctggata ctcatttctt
     4141 caatgctgat ctgactgatt atgagcaggc taaaaaggtt attggtacga ctgagtatgt
     4201 agttcatctg gctgatgttg tggctggcat tgactatgtg tttaagaacc agggcgaatt
     4261 gttccgaatc aataatctga tcaattctaa tgtttttgat tgttgccgta aggctggcaa
     4321 ggagaaaatc aagggtgtta tctatgttgg taccgtatgc tccttccctc tgaccagaca
     4381 gaatactttg aatcctgagc ctctccgtga agaggagttg ttccctgctc ttcctgagtc
     4441 tgcttatggc tggagcaaac tgatgggcca gttggagatg gggtatttgg aaaaagaaac
     4501 cggaattccg tgctgtatcc tgatgctcca taatgtttat ggcacaccta ctgattttgg
     4561 cgaacgtagt caggttattc ctgctctgat tcgtaaagca atcaataatc ctgaagagcc
     4621 ttttaatgtg tggggtagcg gagagcaggg acgtgctttc attcatgtca atgatatcgt
     4681 aaatgcattg gtgctcgccc tggataaggg ttggggtcat ggtcatattc agattggccc
     4741 ttctcactgt acttccatca aggaaattgc atataagatc attgaaattt ctggcaaaga
     4801 catcaagcct ttctttgata cttctaagcc tgaaggtgat aaggctcgtt gcgcagatta
     4861 ttctaaggcg aaagagattt tggattggga gcctactgtt tctttggaag agggtcttcg
     4921 tgaatcctac aattggattg aaaagcagat taagggttaa taagtatata agctgttctt
     4981 cctccgtgta attagaggat gcggaggaag aacatatgag taaggatgta ctgagatgga
     5041 aaagaatatg tataaaagaa aacgtgattt cactgtgtgt ggaatagtag gtgtttgtgc
     5101 tactacaaag aacgagtttg taagggaagt tttttccatg ccaacatctc cggtaagtgc
     5161 gactgttgat ctcgtgggag tgccggccat tatttcggca aaggaaaatc ccaaaattgc
     5221 tgaaatgtat agccaggcta caatggctgc aatagatggt atgcctattg ttaaaattgg
     5281 acggcgcaaa ggatttacct gtgaacgatg tgctgcacca gatattatgg gaatggtttt
     5341 tgaagaaggc gttagacagg gaaaaacaca ttatttctat ggtggaaaag ataacgaagt
     5401 gttaaataag cttcgtgcaa acctagaaag agattacccg ggaatcaaaa ttgttggaat
     5461 gtattcccca ccatttcgtc cgttaaatga agaagaggat agcaagattt gcgaggaaat
     5521 caacaactta aatccagatt ttctgtgggt tggtattggt gcgcctaagc aagaaatgtg
     5581 gatgtgggaa caccagaaga agatccataa cacagtaatg ttcggtgtag gcgccggctt
     5641 taactttttt gcgggcactt tggaaaaagc tcctgaatgg atggaaaaag ctagtctaga
     5701 gtggttattt agactggtaa aggaacctcg tagactatgg aggcggtata tcttgggagg
     5761 tataaagtat atctactata ctattgaatc taagtagggg ggcaataaaa atagatgaaa
     5821 atacttatag tagttacaat gacgaataac tttggtaaga aggggtccta taattctcag
     5881 gaaataggat tagctcgagg gttagctgaa ttaggccacg aagttattgt atataaaagt
     5941 gtaaaaagtg atgaggaagt tggaacagag caattaagca atacggcgat gattcactat
     6001 atcaagtgtc gttatgtagg atcaaatgga ttcttatcaa caaagaattt tgataagaat
     6061 gcggatgttc taatacaatt tgcggacctt caactatcag tacctcatgt atactcatgg
     6121 actgtaaaaa atggaataaa atatatacct tataatggga taacgcgaag tcaaagtggc
     6181 aataaatata taaagacaat tattgaaata ttggcgcatc gaaattataa aatttataag
     6241 aaaaatgggt gtgttgccaa aaatgatcga gtttctgaaa aattagtaca attaggagta
     6301 aataaagtga aacttgctcc tgtttgtgtg gattttgaac agttacataa gaattacgaa
     6361 gcaatagatt ttactactct cagagaaaaa tggaatattc ctcgcaatgt tcgtgttatt
     6421 ttatatgtag gacggtttga aaaggataaa agaccgttag atatgattag tttatttaat
     6481 aaacttgatg agcataatta tttgctgatg gtaggaactg gtgctttact cgagcaaacg
     6541 aagcagcaga taaatttact tggactgcag aatagagtaa aaataattga gcgtattcct
     6601 aacaaagata tttgggagtt atattgctta tcggatgtat ttgtaaatct aaactatact
     6661 gaaatttggg gtatggtttt gctagaggca atgtactatc gcctcgggat tattgctgtt
     6721 aatgcacctg gcccacagaa tattattttg gataatagat atggaatgtt ggctaaatct
     6781 gacgatgaaa ttttgacatt cttaaaagac ggacgattta ccaacgatat catagatgca
     6841 gcatatcagc gtgtaactac agaatttaga tggtcaaagt cgggagaaat tatgcttagc
     6901 atgttggaag gtgataaatg atatggaagt aacgaaatta atggcattga ggaatcgata
     6961 tgctttaaac atagttgata attgcacgag aaaaatagcc aaaattcttg gttgttgtat
     7021 tggtaaaggt gcacagatag ggaactctgt tgaatttgtt cataattctg taggcactgt
     7081 gattcattct gacacaatcc ttgaagatgg tgttaaagtc tatcagaatg tgacgtgtgg
     7141 ttgagcagat agtaatgaac gtatcatcat tcgagtaaaa aagaacgctg ttttatgtgt
     7201 gggtgctaaa attctttgta aatctaacac agaattatgc gttggggaag gtgctgtagt
     7261 tgctgcaaat acagtggtat tgaggtctat tccaccctac gaagtgtggg gggtacctgc
     7321 taaaaagatg gggagataaa acatgaatgc atataaatta tacaaagtag aacgatggtt
     7381 ttacttacac aaaatggagt tttttgccaa gattgtaaag gcactaattt atgtacttca
     7441 taatagctca atttcatatc aaacagaaat tggagataat tgtaagttct tatacggtgg
     7501 cataggttgt gtaattggaa aagatacaaa aataggtaat cacgttataa tcggaacgaa
     7561 tgtactgact ggtggtagat ccaataagcc tggtatgcct acaattggaa acaatgtata
     7621 tatcgctact ggcgcaaaaa ttttaggtaa tatcacaata ggggacaatg taatagtagg
     7681 ggctaatgct gtggtcatta atgatgttga acctaattgt tcggtaggag gcgttccggc
     7741 tagagtattg aaaaaagata ttgatgtaaa tgattattgt agtttgaatc gttactaaaa
     7801 ggagagattg tatgttgtat tttgttattc ctttaatgag tgcgaaggca gcaaacaatt
     7861 gggagatggt tagtacctta tttaaccgaa cattatggtc gtgttataat cagactgatc
     7921 ctgatttcaa aatcattgtt gcttgccatg aaattcctaa actcacgcat gaatatgatg
     7981 acagagttga atttattcaa gttagcgaaa aggatgcccc tattcccaca aatcaagacg
     8041 aaaaaatgat tgataagggt tataaaacac atgctctcgc aatgcgtttg cgagagttgg
     8101 gcggcggttt cgctatgatg gttgacgcag atgatcttgt aagttctcat ttggcggaat
     8161 atgtgaattc ccatccccag gaaaatggat tttatgtcaa aacgggatac gtgtactttg
     8221 ttggagacga ttatatgaag gttctaccaa agttctcatc aggaagtgcg tgtatagtaa
     8281 aatatgcagt ggaagacctg cctgaggcat atccggaagt tatgacagca aattgtgatg
     8341 ataatccatg gatcattaga aaacgacatg ggggcgttgt tccagcatgt gaagagcaag
     8401 ggcgtccatt aaaacctctt ccattcaaag cagcagtata tgtattagga acaggggaca
     8461 atcactcgct ttatggtaaa acgacaaaat atcagacgaa aatgcgagag atactggaaa
     8521 tgtttatagc aaaacgtaaa attaaaggaa aactgaaagc agaattttct gttgattggt
     8581 tataaggtga gtattatgaa ggtattgatg tgtgaaagcc aaatgccaga gaattggtat
     8641 tcgtttgatc tttctaaaga acttaatcaa tatgttgatt taacattgct gtgtaagcgt
     8701 gatgttcctc ctttagatcc taaaattaag agcaaacata ttttgtatca taaaagtaca
     8761 aataaagtgt tttcggcact tgtttttctg aaaagcttgt tcttaatttg gaaggaaata
     8821 agatttggtg attatgatat tgttcatttg caattgatga aaagtctcaa atatgaaggc
     8881 ggattgtacg ttttatgtaa gagacactat aaaaaattaa ttatgacagt tcataatgtg
     8941 ttaccacatg aggttcgtga aaaggatcgc caagagcact ttaatatata ttcaaattgt
     9001 gatggtttca ttgttcacaa tcgagtaaca gaacagcatt tatatgctga attcccagaa
     9061 ttaaatgcaa agtcatatgt ttgtccacat gctatctatg gagatgttaa acatatatct
     9121 agagcgtatg ataaagataa gtttgaattt atcttgtttg gacaaattcg tccatataag
     9181 ggaattgatg tactgcttaa tgcaatcaca gaacttccaa tggatgtaag aaagaaagtt
     9241 cattttacca tagcaggtca acaacatcct aaacaggata gtactgacta taaaaaaatg
     9301 attgaagatt taaaggtttc agattgtgtt ctatataagc cagagagaat ttctgatgaa
     9361 gaaatggaca gtatgttcag aaatgcagat gccgcaatat ttccatataa agaaatctat
     9421 ggaagtggag cgcttcttat ggcatacact tattgcttac caactattgt tagtaatgta
     9481 cctgctttta tggaagaaac cgaggaagga aagatagggt tacttttcga aaatttgaat
     9541 tctaagagcc tagcagacac aattgtaaag tatgtgaacc ttagcccagc tgagaaaagc
     9601 aaaatagagg atgatatata tgagcttgta aaccgcaagt atacctgggc ggcatctgca
     9661 aaactcacat acgaggcata caaacaaatt ttgaatgagt agtaaaacaa ttagaaagga
     9721 ataataatta acatgcagat tagtaaatgg aaaatcatac agtttctaat ggtctttcta
     9781 atgattatct catcaagcat attgaagcct ttgggcggct ggttgggaga aacgatgaac
     9841 gttagacttg tgacagtcat tctagcaata gtatgtttgt taatagcctc attaccagca
     9901 tttagaaaaa atagatattt aaatttctgc actttactga tttgtgttgt tataatagca
     9961 gcttacatct actcagataa gacaatatgg gttaaatcta gggaaagtat gagtttatac
    10021 tatgtattat tggcgtatcc attgttctgt actttaatta accaagcgtg gaatttggat
    10081 tcaatgctaa atacgatatg tggtgtaaca tttctttcat acattcttag aacatctatt
    10141 tcacttattc agaaattttc aggagtactt ctctatgaaa acatatatcg tgagtgtgcc
    10201 ccggaaaatt ggtatcgtgg aggcttttta agaattaatc caccatgttt tacaattata
    10261 ataataccga ttgctttgta tatgattgag cgacaagttg tagctagaag aaaagtaaaa
    10321 tattatttac tgattgctag cgcactggca tattctgctg ttattcatgg ttctagatct
    10381 gtccttgtct atcaaattat tgtacttggt tgctatatcc tttttaagaa aggttcttcg
    10441 aaaagaaaaa ttgcaatgtg ggttttagct gggttgcttg tagtcatatt tattaattcg
    10501 accatgttct caacatttgt tgaaactttc acaaacagta caggtgaata tgcaggatct
    10561 gctgaatctc gatttgcttc agtttggttt tttattccta aatttttgca atcgcctttg
    10621 ttcggtactg gtattcttac tggtgaggaa tttacgttta tattaccagg aggagttaga
    10681 ggacacctat ctgatattgg atttttctat acaattactc aatatggtgt aggtatactt
    10741 atatttgata ttctgtttat tataaaaggg ttcaaaacag cattacttgc atcaaaagtg
    10801 ggtatgactg gctatcaata tttagcattt ggaattacat taaatgtctt gctaacaggg
    10861 attaatatag actggtttta tccgatattt actccagcta ttcctgtatg cttagcggtt
    10921 atagaataca tctatcaaga atgtagaagt gtggctaaca tagaatagga aatattaata
    10981 ggagtgcatc tcatgaataa gaaagagatt ttatataaca tattgaagaa gtacgaaaat
    11041 ttaccatatg tgtttaggaa catgtttggt cataatataa gtaaactcgt atcttggaaa
    11101 ttgactgctg attgtgcaac agtagaagaa aattctgaag tttatgcagc gtttcaattg
    11161 cgctcaaagg acatgtctga tgttccagtg atgggtttag ttaattgttg caaagatgta
    11221 gcaattgtaa tgcaggggcc actatgttta aaggatgatt tctcttataa tactctggcc
    11281 tactataaga aatgttatcc aaatgcttta attattcttt cgacatggaa ggacgaagat
    11341 attaaggcat tgaaaaggat agaggatctt ggagttatag ttatttgtag tgagaaacct
    11401 gaaaagcctg gacacttaaa tattaattat caagttggaa acactttgcc tggaatagtc
    11461 agagcaaagc agcttggtgc aaagtatgtt tgtaaaacta gaacagatca aagaatatac
    11521 catccaaatg caatggcgtt tttctgcagt ttacttgaac agtatcctgt taataacgaa
    11581 gatgattgga aattaataca aaatcagcga ttactgttac tatcaatgcc gtatggagac
    11641 atgtttttcc cgtattgttt gtcagacttc ctgtattttg gtgatgttga tgatatgata
    11701 aacctcttct cgattcctag tgatgtgaga gaaaagggag ctgtgtctag aggagtctca
    11761 agaagggaga tatctgaaaa taatttagct ccagaagttc agcttcttcg aagctatatt
    11821 tcacgaatgg ggggcaatga ggagtgctct attagggcat attgggagtt tgtaaaaaac
    11881 catgttatta ctattaacaa aaatcaaatt gatttgtact ggcataaata tgtaggtcgt
    11941 tattcaaata atactattta tggtacgtat tatattgatg atagccaaga tgcacttcac
    12001 tgctataatt ttgattttat aaattggttg aatttgtaca ctggaaaata cgaatacaaa
    12061 gcagaatatg aaaaatatat ggactttgtt tgggaagagt aattaacttt tatagtaaaa
    12121 agtactttag ggaagggcat attatgaaaa cagtaattat ggcgggcgga cgaggaagcc
    12181 gaatttcgga attgtttccg gatattccta agccaatgat tcctatagat ggcattccag
    12241 ttttagaacg agaattgtgt tcgcttagag atcaaggctt taatgatatt attttgactg
    12301 tttcctattt gcatgaaaaa atagaggagc atttcggaga tggttcaaaa ttaggaatta
    12361 aaatcaaata ttttattgaa aaaactccac ttggcaatgc aggtgcattg tattttttgt
    12421 tggatgaact gggtgatgaa ccgtttttac ttttgaatgc tgatgcagta tttgatgttg
    12481 atttcaggca gatgattaga tttcatgttg agaataacgc agatgttact ttgtttacgc
    12541 atcctaactc acatccgtat gatagtggat taattatatc cgatgacaat aagaaagtgc
    12601 ttcaatggtt agcgaaggaa gatcaaagac ctgaatatta taaaaaccga gtaaatgcag
    12661 gtcttcatgt tataaatccg aaggtgttga gagaaaatcc attaatggaa attgtaaata
    12721 aatcaatagt tgaaggtggg gggctaccat acaaaattga tttagataga atgattctga
    12781 aaccattggc taacacaggg cgtatgtatt gctacgattc tccagagtat gttaaagata
    12841 tgggaacacc ggaccgatat agagctgtaa ttaaggactt tcaaaaggga attattgttg
    12901 gtaaaaatct atccaatcca caaaaagcaa tctttttaga tagagatgga acgattaata
    12961 aatacgttgg ttttttgaga aatgcagaag aatttgagtt aattgatgga gtatcggagg
    13021 caattaaaat tattaattct ctaggttttc tgtgtattgt tataacaaat cagcctgtaa
    13081 ttgcaagagg tgaagttact gttgatgagc ttaatagtat tcacaataag cttgaaacaa
    13141 tgctagggca tgctggtgga tttttaaacg gtatttatta ctgtccgcac catccccata
    13201 aaggatatgc aggagaggtt atagaattaa aaattgattg caattgtcgc aagccaaaac
    13261 ctggactgtt attagaagca tcaaaggatt ttaacataaa cctaagtcaa tcatttatgg
    13321 ttggagatag tgaaagtgat gtaattgctg gaaaaaatgc tggttgtaag acagtactga
    13381 ttacagaaga catcaccttg cctgctaatt atggccaaga ctatagtgtc tgttcattgt
    13441 tagaatttgt taaaaatcat ttatgcacta tgcaataatt ggtttagtga aacttgtaaa
    13501 aggagtaatt agatgaaggt attaattcca gcagcaaaag ttgttgaaaa agaattgcag
    13561 agtataggaa tgttaccagc aattatctat ccaattaata aaaaaattgt ctttgattat
    13621 ttgtataatc agtataatgc aatctgcgat tcattggata ttatctgtta tgaaaaagcg
    13681 gataaggttc atagaagact gaaaaactat catgattcca gaattcatat atgtgacctt
    13741 cctgaattag gggatctagg gcaaaccgtg tattttgggt tatcagatga agatgagcct
    13801 gttctaatta acttcgcaga tacaattgtt atggagagta tgtctgatat agaaatggat
    13861 acattctatt attctgaaga ttttgtgtcg agtacttgga catactttaa aattaatgaa
    13921 ggaataatta ctgatatcca cgataaagag gaagcttacg tatccgataa acgtgaaaaa
    13981 ttatttattg gcgtatttat gttttcttcg tcaaaacttc tgaagagttg cttaagagag
    14041 gcagttgaga gtcggaaaca ggggggaata aactcatttt atcgggcttt acagctatac
    14101 agcgaacgtt gtccgctaaa ggccgtaaga acagaaaagt ggtttgatat tggacacatc
    14161 gataaatatt acaatacaaa gttggaagtt aaagcacgag agtttaatca tatctctata
    14221 gataaagatc gagggatatt gaagaaaact agcgatgaca aggataaatt tattggagaa
    14281 ataaagtggt atttaaaact tcctgctgat attgaatatg taagacctag aatttttgat
    14341 tattctacat cttacgtagc tccctatgtt gatatggaat attatgcata tcatacggtt
    14401 catgagttat acttatatgg tgatttaaaa tataaccagt ggcatgatct gtttagccgt
    14461 ataaagttta tctgtagcga ttttaagcga tacaccttaa aagatgaaaa gattagagag
    14521 tcattggaag atatgtattt gcgaaaaacc atgcagagat ttcaaaaatt gtctgaagat
    14581 aaaagatttt cagctttttt tgagaaacaa attcaaatta atggtcgtaa gtttatttct
    14641 ttaaccggtg taattgaact tctaaagaat cttatcccaa gtatgctcta tgatgtaaat
    14701 gaattttgta ttatacatgg agatttgtgt tttgctaata tgatggtaga ttccaattgt
    14761 tcctttgtta aggttattga tcctagggga aatttggaaa atatgatatt tatggggaca
    14821 gcagatatga attagcaaaa ctatttcata gtttagatgg taaatatgat tatatcatca
    14881 aggatctatt tactgtagaa tatgataccg agtgtgcagt gatagattat acaattaata
    14941 cgcgtgtaag agattacgat ttatttgaat tgtttaagtc ggtcttttcg gacgagatta
    15001 aggacgattt tgataagatt gtattgatag aagcgttgct atttcttagt atgattccat
    15061 tacacggtga aagttttgat caccaaatag tgatgcttgc tacaggtatg gaacttcttg
    15121 caagagtaag taataaagtt atactctaat ataaattgtg ccttaatgaa taacggttac
    15181 aatactgttt tagcaatgta ggcattaagg cacgattata ttaattaata caatagaagg
    15241 gaagagaaaa atgtatagtg aatattcttt tgtgtttgat atcgatggca ctttgtgtcc
    15301 gataaagaag aaggatgagc gatatgagga tttggtgccg tatgagaata tggttgaaaa
    15361 actgagattc tataaggaga atggtgctaa aattacattg ttcacctcac gcaatgttaa
    15421 ttcctataaa ggaaatttgg gaatgataaa tgctaatact gcaaaagtgc tcttggcgtg
    15481 gttggataaa tgggacattc cgtatgatga aatcctgtat ggtaagcctt ggccggggca
    15541 taaaggcttc tatgtagatg atcgaacggt tagacctgat gagtttttga gatgctctgt
    15601 agatgaatta aatgaaatct gcaacagttc aaaaaaaaga ttctaaataa ggaggttagt
    15661 tctatggaaa aaaagatgac agttatcatt acgatggcag gtttgggaag tcgttttcga
    15721 aaaattggtt atgcggttcc taaatatatg attaatgcca aaggaagaac tttatttgat
    15781 tgggccatgg atagtttggt tggatacaat aaaaatgttg ataagtatat ttttgttgtt
    15841 cgtcaggaag acaacgcttc agcttttata aaggagcatt gtgcttactt tggtattgaa
    15901 aacgtagaag tgattgaaat agactatatg acagatggtc aagcaactac atgtatgttg
    15961 gctattcctt attgcaatca agacaatgag attatggttt acaatattga tacatacgtg
    16021 gaatcaaatg agatgaaata cgaagatatt tgtggagatg gacatattcc atgctttcat
    16081 gctgatggcg atcattggag ttttgcaaag cttaacgatc gaaatgaagt aatagaagtc
    16141 cgcgaaaaag ttcgcatttc tgataattgt actcttggtg cgtattattt ctcatcggca
    16201 aagctgtata aagctttata tgaagagtat tatgcagact cctctaatat ggagaagaat
    16261 gaaaaatata tagcaccatt atataacttt atgataaaaa aagggttaaa ggtgactatt
    16321 agcatagtag atgcgaaaaa ggttcatgtg cttggtactc cagaagagct caatgtgttt
    16381 ttgaatcaag aataaatagt gtttaggaga aaatagcagt gaccagtaat aatctaattg
    16441 taaaaacatt aagcagatat aagaatactc cacttcctgt taaggctgca ttatggttta
    16501 tggtatgtgg ttttttacag agaggggttt caatgattac aaccccaatt tttactagag
    16561 ttttgtctac agaacaatac ggtttataca gcacttatct atcgtgggaa tccttattgc
    16621 tcatgattac atcgcttagt ttgttcaaag ccatgatgaa tctttatgta aaatatgata
    16681 atcaagaaca tattctttcg acagtatccg gattgacttt agtactgact attttttggt
    16741 ttgttattgc tatttttatt aaggatatat tggcagaaat tttaggattg acaccagttc
    16801 tagttgtctg tatgttctca
//
LOCUS       DACDSY010000060        16428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDSY010000060 DACDSY010000000
VERSION     DACDSY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.060"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..1163
                     /locus_tag="IWK44_001562"
     CDS             6..1163
                     /locus_tag="IWK44_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9550093.1"
                     /translation="MFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVLDFVAR
                     TMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIFTDDNQ
                     LLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTRGLFED
                     YAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIWNSFKT
                     NSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALIYGEKA
                     ELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLEYATQI
                     DKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            1150..1854
                     /locus_tag="IWK44_001563"
     CDS             1150..1854
                     /locus_tag="IWK44_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP9550094.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            1859..3019
                     /locus_tag="IWK44_001564"
     CDS             1859..3019
                     /locus_tag="IWK44_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9550095.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            3095..3373
                     /locus_tag="IWK44_001565"
     CDS             3095..3373
                     /locus_tag="IWK44_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9550096.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            3354..3725
                     /locus_tag="IWK44_001566"
     CDS             3354..3725
                     /locus_tag="IWK44_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP9550097.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            3741..4145
                     /locus_tag="IWK44_001567"
     CDS             3741..4145
                     /locus_tag="IWK44_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550098.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            4142..4552
                     /locus_tag="IWK44_001568"
     CDS             4142..4552
                     /locus_tag="IWK44_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550099.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            4613..5200
                     /locus_tag="IWK44_001569"
     CDS             4613..5200
                     /locus_tag="IWK44_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9550100.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            5273..5740
                     /locus_tag="IWK44_001570"
     CDS             5273..5740
                     /locus_tag="IWK44_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HAP9550101.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            5786..6094
                     /locus_tag="IWK44_001571"
     CDS             5786..6094
                     /locus_tag="IWK44_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550102.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            6289..13023
                     /locus_tag="IWK44_001572"
     CDS             6289..13023
                     /locus_tag="IWK44_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9550103.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            13027..13899
                     /locus_tag="IWK44_001573"
     CDS             13027..13899
                     /locus_tag="IWK44_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550104.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            13896..14523
                     /locus_tag="IWK44_001574"
                     /pseudo
     CDS             13896..14523
                     /locus_tag="IWK44_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14484..14945
                     /locus_tag="IWK44_001575"
     CDS             14484..14945
                     /locus_tag="IWK44_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550105.1"
                     /translation="MQSPGPGNENKLLIVDKAMKDIENPLVLSTWYLDDENNLTQDST
                     NKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGNAYSHEIKVNVVLNARNINVENI
                     ETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFGNVRSRFMDYFEDNGG"
     gene            14949..15773
                     /locus_tag="IWK44_001576"
     CDS             14949..15773
                     /locus_tag="IWK44_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550106.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            15791..16336
                     /locus_tag="IWK44_001577"
                     /pseudo
     CDS             15791..16336
                     /locus_tag="IWK44_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
BASE COUNT         5873 a         2533 c         3513 g         4509 t
ORIGIN      
        1 ttgatatctt taagcagtca ataagaaatg aagagccttc tgagtggata ccggattttg
       61 tatatggaga ggaagtatct gctcgggcat acctcaaaat tatggcaaaa aatactgttt
      121 tagattttgt tgcaagaact atgtcaacgt tagaagttaa gtttaaaaat aaagatggga
      181 catcagactg ggattatatt ctaaatgttc gacctaattc tgatatgtca gcagctacat
      241 tttggcaaaa atttttttat cggctcttag atgacaacga agtgttagta atatttacag
      301 atgataatca attgctgatt gctgatgatt ttagtcgaac ggagtatgcc gtgtatgatg
      361 atgtttttac aaatgtgatg gtaaaaaatt ttgtctttga acgttcatgg aacatgtctg
      421 acgtcattta tatggaatat aataacgaag aattagaaag gtttacacga ggtttatttg
      481 aagattacgc cgcgttgttc ggacggattc tagaagttgc aatgcgtaac aaccaaattc
      541 gtgggtctgt atccattgat tcaacgggaa cggcgaatga tgaaaaagga aaagatggta
      601 agactagatc tcaaagatta caagaatata ttgataggat ttggaactct tttaaaacta
      661 actcagttgc aattgttccc aaagtgaaag gctttgagta tgaggaatac accaataaac
      721 aaggcgtatc gaatcaatcg ctagatgaac tgaataagat gaagtcgtct ttaattgatg
      781 atgttgccaa cgccatagga gtacctacgg cgcttattta tggtgaaaaa gctgaactgg
      841 attcaaatat taaagctttt cgtaaattgt gtattgctcc actagttaaa aaacttcaag
      901 acgaattggt ggcaaaaata attagtcgtc aagagtacaa aaacggggaa cgtatagaag
      961 ttaaaaaagt tctccaaaaa gatctattag agtatgctac tcaaattgat aaaataacat
     1021 ctggtggtgc gttttatatt gacgaagttc gtgatgaagt tgattatgat gagttgccaa
     1081 ataatgaagg aaaaattcgt attcgtacga aaaactatga agaaactgtg aagggaggtg
     1141 agaacgacaa tgccgaaagc taaaaaagta ccatttcagt ttactaacga aattcagaat
     1201 ggtaagcaca ttctcacctt gagtggaaat gtacagaaaa aatattggcg tgatgatgat
     1261 gtcataaacg ctaaagatat tcgtgactct ctagatactg taaccgatga tattgtaatc
     1321 aagttgaaca gcccaggcgg tgatgtgttt gaagggattg agatttacaa ttatttaaaa
     1381 gatcacccat caaatattac tgtagaagtt acagggcttg ccgcaagcgc agcaacgttt
     1441 attgtcgctg gtgccaatga tgtgattatg aatgttggga cttctttgat gattcatgaa
     1501 gcttctacat ttgcttgggg gaataaacag gacatccaaa aaacgttaaa cgcattggaa
     1561 acgatcgatg attctatctt agctatttat tcagataaga ctggccaatc tgttgataag
     1621 ttgcgtgaat ggatgaatga agaaaaatgg tttacagcag atgaagctgt caaatatgga
     1681 tttgccaatt ctgtgaagcg tgaagaacca caaaatgaac ctcaaaatat tgcagcgatg
     1741 attcaagatg ctgttgccgt tgcaatagcg aatttaaagg aacctgttac taataaaatc
     1801 gaacaagaat caaaacaaaa atcattaatc gcacgattgc gaaaaggaga ataaagatat
     1861 gttaaaaatt acagataaaa ctgcagatgc aaaaaaggca tttaatgcta tttcttcgaa
     1921 agaagatgca acacccgaac aaattaatgc tgctctagaa gaatatgtaa ccgccattgc
     1981 agaagatgcc ggcaaacagg tgcgtgctga atatgaagaa ttgaaaaatg tgacggacaa
     2041 ccgtgtatta gaggctcgtg gaattcctac actaacagca gaagaaacaa agttttataa
     2101 tgaagccgta aaatctggag gtttcgattc tgacgtagtt tggccagaaa cgattttgga
     2161 acgtgttttt gaaaatcttc aaaacgatca tcctatttta ggcctcatta actttacacc
     2221 aactgtaggt cgtgtcaaag taattagagc acgtcgtaaa ggtgttgctg tttttggacc
     2281 attgcataaa gaccttgaag gccaactaga tgctgaattt ggtgcaactg aatttgttca
     2341 acttgcgttg accgcatttt tcttaatttc taatgatacc ttagatttag gaccacgatg
     2401 gattgaccgt tttatcaatc tttctttaag tgaagcagta cgtgatgttt gggctaaaaa
     2461 aattattaca ggttctggta aagatgagcc aattggattg cttaaagatc tagatggtgc
     2521 ggtcacaagt ggtgtatatc cagataaagc tagcgcaggg acattaactt ttgcgaaaga
     2581 gaagattgtt cttgaacttg caggggtgat gaaaaaatta tctaaataca catacaaagt
     2641 agatggtgac gatgaaggtg aagtgaaata ccgttctgtt gctggacgag tgtatctaat
     2701 tgttaatcct gttgattact gggatatcgt agctagagtt acatttgcaa atttaaacaa
     2761 tgtgtatggg actacgatgc catttatcgc tgttgatcat atcatcgaat ctgtagacgt
     2821 accagaaagt aaatgtattg catacgttga taaagagtat gaagctactc aatcaagagc
     2881 tgaaaaagtc tatgtatata aagaaacttt tgcaatgaaa cgcgcaactt tgtatgctgt
     2941 cgacatgctt ggtaatggct atccaacaaa taatgatgca gcacaagtat atgacttgga
     3001 ctttacagcg ggggagtaat gtcccctgca attggtgaga taacaccgac gactgatggg
     3061 gcagtcatta atctttcgta aatggggtta gagtatgaac actgaagaat ttataaaaga
     3121 atataaggcc cgatttcgta ttttccatac ttccgaagat gaggatatcg ggaaacagtt
     3181 agagagcggg tttgccgata taaaatctct aatcggcagt tttgatcctt ccacttatga
     3241 gaaaggaaaa gaattagttt atgaacgtac tcggtatcta cggaacgaat cactagagta
     3301 cttttacgac aattttcaaa caatgattat ggatgcatca attgatctgg caggtgctgg
     3361 atatgccaat taaatcaaat tataagaagc ctaaaattgt agctggtgat ctaaatacgc
     3421 cagttacttt ttttgagttt caacctaata agggtccaga acctggtgat gaagaaaaaa
     3481 aggaactgta tttttgtaca gcactagttt acaacccatc aatgaaagat cgtgacattc
     3541 ttggcacaaa agaaggcgtt acgataaaaa tacgggatcc ccatcaatct tatattccta
     3601 ctaacaaaca taaagtaagg atattagatt atcgatatgg agataaagtt tgggaaataa
     3661 tcgatgtctc accggatttg gaagaaaatt caattgtaaa gatagtatta gaattaatta
     3721 cttaaatttg gtgataaatg atgagtaacg taaagtttga aggcgttcaa gaaacaatcc
     3781 aagctttaga aaaaaagtat ggagaaaaga aaactaagac attaacaaaa aaagcaatca
     3841 atgttggcgc tgaaaaagta gaaaaacagt tgcaaacgga catgaccgtt tttaaagaca
     3901 aaggttatac gatcgctgag gttgttcgca agaacgctac gtatagagat tacaaggcag
     3961 aagctgaaat cggttggaat ggtccacatc aacgatatag aatcattcat ttgaatgaat
     4021 ggggatatac acgaaagggc aaacaaatta gaccgagagg atttggtgta ataacaaaat
     4081 ctcttaagaa ttccgagctg ttgtactttg atgctgtagg agaggaggtt aaaaagagtt
     4141 tatgaaagat atgcttaata tcatttacga ggctctttgt ttgaatgagt atattcattc
     4201 aatgacctac aacgagaata ccgagcaata tcgtattaaa ttttatgagc aacctgaaac
     4261 cgccgataaa acaggtccaa tgatcacaat tcgtcctgta gatacaccta acgaagcgta
     4321 ccacggaagt aacaaagagc tttctgtgga gcatatgata cagattgacg ttgaatcgaa
     4381 atatagagca acatgcaagc agattcaata tgagataaaa aaagagatga aaaaactagg
     4441 ctttggccaa atttccggtc aagggttaga tgaatacttt actgaaacaa accgctttgt
     4501 agacgcgcgt cgttacgaag gaaatacaaa aatttacgat accgaatatt agaaattaag
     4561 acacgaaaat aacgtgtctt tttttattac ccaaaattag gaggaaaatt ttatgacttt
     4621 agtaggattc aaaaaaatga caatcggggt ctttgacagc acagggaaga ttccagcagc
     4681 aaatttatat gtaattgaag gtgaacaaaa caaaggggcg actgtatctg ctgaaatcag
     4741 tgggctatca aaagaaccat caaaagttta cggatcgaac atcgcttact atgtctctca
     4801 aaaaggaaca ggcgatgttt cagctacttt tggattgtta gatttaccaa cagaagtaaa
     4861 tgacaaaatt cttggttaca aaaccgatac aaacaaaatc agcttcttgg gagaagatac
     4921 tgagccacca tattgtgcaa ttttgatgga atcggaggat ttgaacggcg atacagcaat
     4981 gcttgcgatg ttcaaaggaa aattcagtcg tgaatctatt aatcttaaca ctacgacaaa
     5041 tgaagctttc gaaccagaag cagaagaata tgtattttca gcgattgcca atgatgttga
     5101 aggtgatgct aaaggccaaa cagtcgcaaa atatgttgga gacgaagaag cttctattac
     5161 tggtttgaaa gagatggtat ttcctgcagc gggggagtaa ctagccccgt tgttggagca
     5221 atcactccca ctattaatgg ggcaacagtt gaattaagtt aggaggataa ctatgccaga
     5281 tacatttaaa atctataaaa aaaatggtac tgaatttaca aaagtcgcag agggtgaaag
     5341 tccactttct attactggaa ttgcagcaaa tactcaagtt gctaaaggag attatcaagc
     5401 tacgcgtgtg gtcggaggtc aagaatcagt aaaagtagat attcctgcat tcaccactct
     5461 tccaatttca gttactggtg tgacgttgga taaaacgaca gctgaagttg aacaaggcgg
     5521 aaccttaaaa ttaactccta ctgtaacacc agcaaatgca acagacaaaa caggaagctg
     5581 gagcagttcg aatacagctg tagctacggt tactggaggc aacgttacag taaaaacaga
     5641 cgctgaagtt ggcggaacta cagaagtttc atttactact acggatggcg gtaaagtagc
     5701 aaaatgtact attacagtta ttgaaaaagc agcgggctag tcttcggact agctttttta
     5761 tttggaaaat aatggaggaa tctaaatggc aaaagtaaga attgaattga aagaagaatc
     5821 aggaaagaaa gtctatgaaa acatagacac aacagggaaa gattatcgaa aagctttaga
     5881 aacaatcaaa aagctaaatg aagacggagt aatgatttgg aatcgtttag acatttattt
     5941 ggattttgct gtaggtatct tcagggatag caaattgaca gctgaacaaa ttcttgatgg
     6001 cttaccatct gagaaggtaa tgagcacatt ggacgacatc ctaggagaag taatggggat
     6061 tgaaagtaat ccagacccag acgcaaaaaa gtaacacccg aagaagcaga agaaatgtac
     6121 ttaaacctat gcagagagtt agtgaaagcg ggatggtcac tatctgacat agaagataat
     6181 tcctttgaaa tgatgatgaa aattgcgtgc acttcgccta agaaagagaa acaaaaagaa
     6241 gttgatctta aagacttctt gaaatccatg tagagaggag gtaaacatat ggcaaatgga
     6301 aaacctttag gcaatatgat tatcaaacta gacttagaca gttcagcttt ttcaaaaggt
     6361 ctagccggag ctaaaaacgc tgttaaccat caaatgaaag caatgaaatc acagatgcaa
     6421 gtaatgaact cttcaggaaa ttctctcggt gctttacaag caaaatacaa tggtttaggc
     6481 ggagttctac aagcaaatga aaagcaagta gaattgctca ctaaggctta taaggatagt
     6541 tttgactcta acggaaacgc tactgcttcg actgctaaat acgctaatca attgaatcaa
     6601 gcaacagcta gatcagcaag ttacgaatct cagatgaaaa caactgttgg acagattgct
     6661 cggatgaaag tggaaacgga agggtttacg gggaaactaa aggctaattc tgatgtattg
     6721 attaattctg ggaaaaaaat tgaagccttt ggtaaaggag tttctgggat aggatctgcc
     6781 ctaactgttg gagtaacagc tccaatagtg gcaggcgcaa ctgctgttac tagtgcagct
     6841 atttcatggg aaagtgcttt tgcaggtgta aaaaagacaa atgatgaagt tgtagactcg
     6901 aatggaaacg tagtttattc ctatagcgac ttagagaaag gtctgcgtaa tcttgccacc
     6961 caattacctg gtagccatca agaaatagca aatgtagccg aagcagccgg gcaattaggt
     7021 ataaagactg aaaacgtagt tagcttcact aaaacgatga tagacatggg tgaatcgact
     7081 aatatgtcgg ctgaaactgc ggcaacttct ttagcacgat ttgctaatat tacccaaatg
     7141 tctcaaaaag actttgataa attaggatca gtcattgttg atctaggtaa caattttgct
     7201 acaaccgaat cagaaatcac tgaaatggga ttgcggttag cgggagctgg taaacagatt
     7261 ggaatgagcc aaggcgaaat tctaggattt gctactgctc tatcttctgt tggtgtcgaa
     7321 gctgaagcgg gtgggtctgc cttttcgaag gtcatgatcc agatgcaact agctgtagaa
     7381 aaaggatttg gcgcttttga tcagctgaaa cagatggcag aagaacaagg tgttccgtgg
     7441 gttaatttag taaatgctgt gagagacggt ggaaaatcgt taaaagcagt atcagagcaa
     7501 atgggattca cttcatctga tttaaagaaa atgtataaag aagcggacaa ttcgaaaagt
     7561 tcgcttgaaa attttgcgaa tgtggcaggt gttacaagtg aacaattcca gaagttgttc
     7621 aagagtgatc cttcagaagc aatcatcaag ttcattcaag ggctgaaaga ttcagaaaaa
     7681 catggtactt cagcaatcaa agttctggat gacatggata taaaagaagt tcgactacgc
     7741 gacagtttat tgagagctgc aaatgctagt gacatattca aaggagcaat tgaaaaagga
     7801 aacggcgcgt ggaaacaaaa tacagcttta acaaatgaag ctaataaaag atacgaaaca
     7861 actgaatcaa agctaaagat attgaaaaac gaagtgattg atacagcaat cgatcttgga
     7921 ggtccatttg tagacgcgtt gcgtgatggt ctaaatgtat cgaaaccatt gataaaaaac
     7981 ttagggaata tggcgaaagc tttctcagct atgagtgatg atcaacaaag aaacattatc
     8041 aaatgggttg gattagcagc agcagctggt ccagcgctga agcttttagg aactggtact
     8101 tcagtaatag gtaaagttac ttcaactact ggaagattga cgaaaagcct tgttgattta
     8161 gcagcgaaag cagcagaaaa gaaggctatg gatgcctttg catcgtcggt aacaacagct
     8221 ggtgctgcag ctgcaaagac agcaggagct ggaggtattg gcgggttagt ccctatgatg
     8281 ggaaaaacag ctagtgctgc aagtactgct gcgggcgcca gcggaatagg cgcaatgaca
     8341 acgtcactag gtttgttagg tcctgctcta cttggtattg ttggcgttgg tggcgcactg
     8401 gcagttggct atggagcatg gaaattattt ggtgaagaag cttggaattc ctcgcaaaga
     8461 gtaaagcaat ggggaacaga tgtcggtgag gagatagata gtactcttga cggagtacag
     8521 aagaatattg aaggtgcaaa tggtcaattc ggtcttctca aagagggttt tactgttgat
     8581 gatgcaaata atatggcggc taatttcgca accgctggaa catctttgga aacatcttta
     8641 acgggtagaa taaaggcatt agacgatgca ctaaaagggc tacctcaatc agttcaggat
     8701 gccatgaaag aagtagctga atcagaagaa gaaaccttag ggcaatctct ttctaaaata
     8761 aaagacaaca atgaagaaat aaaaaaaatc agagaaagag ccgcaaatca gaacagagac
     8821 ttgactgttt cagaactaca acagatacag gatctaatga aaggaacttc tcaagcgtat
     8881 gtggatacgc tcgatgtttc tgctaaagac agaaaaaaga tactatcagc catgaatggt
     8941 gatgttgcac aagctacaga agaagaagcg aagacgtggt tacaatcttt agcgaaacaa
     9001 aaagctgcaa ctaccgaaaa ttatagacaa atgcttgaag aaaaaaaggc ggctttaaag
     9061 gaaaagggag taagcaaaga attcattgat gcattagaag atgaaatgaa tgattacatc
     9121 aaaactacaa atcaaggatt cgatagccaa atagctgcta taacaaataa atatccacaa
     9181 ttgattaacg aagtgagttt atcaaatggt caattattta gcaaaacggc agctgctatg
     9241 gacaatactg gtcagtatac aaaagttttt ctcgaaagta atgaaaagat tttagcccaa
     9301 gcgaagaaaa catcggatga aatagcagat aatgccgaaa aaaatgcaga acaacttggt
     9361 atggtagctg atgaggctac gcatgctgga tcaacttgga atggcttagt tttagacgaa
     9421 aaaacaggaa aagtaaaaac taatgtacaa gaagtagtga atgaagcaac gaaggatttc
     9481 actaaatgga atgaattgaa acctgttgtt catgatgcga aactaacaac gaatgcaaaa
     9541 gatgtcattt ccatagcggc agtacaaaat ggctattggg atgatatgga atggaaagaa
     9601 aaagaagcat tattagaaga tgagtgttct aaaaatgtat acaaggcttt agaaaatgct
     9661 ggtaaatgga atgagttaga tattccttct aaagaagcaa tcctaacttc aaatactcct
     9721 gaagttatgg gtgaaactct catgaatttg ggattatggg atacttacaa acttcaagtg
     9781 aaagatttag atttgaatgc gtataaagta tacgatgcta tcgatggatc agaagaaaaa
     9841 ttaaatagtt gggctcaatt agataacccc acaaaagaat tgttattaga taactcagat
     9901 ttttctacaa aactttttaa ttccgaagaa atgtttaaac gttggaatca gttatcatct
     9961 aatgcaaagt ttctttcggc taataatact aaatttacag aggttctttt atcatctgac
    10021 agaatgtgga atcaatgggt aaaccttcct gataatgaga aaagattact tgcagataac
    10081 gaagatttag ctaccactgt tttatcttca gaagaaatat acggaagatg gactcaatta
    10141 ccagataatc aaaaatatat gttagctgac aatactgact tagcaaacaa aatattcgct
    10201 tcagaagaat catggaatgc atggaaagca attccagaca cagttaaaaa cttgcttggt
    10261 aataatattg atttactggc taaagtgaaa gacgggactg taagtgtaga agaatataac
    10321 aaagttatgc cacttcttaa aactctatat ggagataacg ttaatttaaa agaggcaatc
    10381 agtggggcta aaagctctat agagaattac aacaaaaata tttttcctag tgataaaact
    10441 gcagtaggac acgatgaggc ttcacaagct gctaaagatg catttgaagc gttcaacatc
    10501 ttccaaacaa aaataccaga taaaattact aaaactgtat cagctgactt tataggtcct
    10561 atgccgaaaa atgcgaaagg aacaaacttc catcctggcg gagcagctat ggtaaacgat
    10621 caaaaagggc ctacttatga agagcttata accttgccaa gcggagaaac ttttatacct
    10681 aaaggacgta atgtcgtttt agacttacca agaggttcga aagtactgaa cgccactaaa
    10741 actaaacgtc tagtgcctaa atatgctgat ggtataggaa acataacgac ggtttcatct
    10801 acgctaaact tagatgcttt aattttagct attaacgagc taacgacggt attgagaact
    10861 caactgatgg caggaacgac tgatgctaaa gatactaaac tatcagatga aacaaaagtt
    10921 gccgatccga ttattccgga cagtctttct gaaaaatcag atcagtatct aggaattgga
    10981 gctcaatggt taactaatct aatgaatggt tggaactcag cggttcctca atatatgaat
    11041 agtgaaatgg tttttattac aaactatctc aatgcgctaa aacttcaaaa caatcctaat
    11101 tatacgcaag gagcaacatg gaataagaac ttactgaatg gatggaatag tttaactggg
    11161 acctttattg ctacaatcaa ttcattttgt aatcaagcaa tggtcacgct tagaaactac
    11221 aacactccta tgtacaacaa tggccgaacg tggcagcaaa ataatcttaa tggttggaat
    11281 tcattgtatg gatcattcat agcccgtgta aatcagcttg gtaacgattc gattaacaac
    11341 cttcgttcta aaagtggtgg tttttacaat gcagggacat tcttgttgca atctttaata
    11401 aacggaatga attcgttagg taattctctt tctactacaa tgaacaatgt cgctaataca
    11461 atggttggtg gtataggcaa aggagttaat ggcgtcatat cgggagttaa ctatgtgctt
    11521 aaggaagtcg aatcaagtaa gaatatcgga aactggccaa ttccacaata cgctaaggga
    11581 actgatggac atcctggtgg acttgctatg attaatgatc aaaaaggcct agttcacgaa
    11641 gaatatgttc agatgccaga tggacgtagt tttattgcca aaggtagaga tcttcttgtt
    11701 aatttgccta aaggtgctca ggtcttaaat gcttctttaa caaagaaatt aaaggaacgt
    11761 ttaaatattc cacactacga aaaaggtatt ggcaatttag atattgttga tttgttagat
    11821 gatgaaaaaa gaatgttaga attcttaact agcaaagttg atttttcagg cattaatgaa
    11881 ccatggcttg atatgactaa atcaggaacg tcattaatgt ccaaagctgc aaatacaatg
    11941 cttcaatcaa aattaagtga attcttcact catggcaatt ttgatggtgc agtaaatgcc
    12001 aatggtgttt atcaatattt agttgatgtg gcacagaaag taatgggtaa gttccccgga
    12061 cttacagtaa cttctggata tcgagcagga gatgcctact atcacggaaa acggcaagct
    12121 atcgacttgg cttatccagg tatttctgga gatccgagat atacagcagc agcaaattac
    12181 gcttttgaaa aattcccttc aaaaattgcg tatgtcatta cgaatggacg tgtacgtgac
    12241 cgcatgggat tatctggaac aaggtcaagc ggacaatgga cgaattggcc agatggcgat
    12301 cacttcgatc atattcattt aaacggttcg atggggtctg gcgatatatt caatggagga
    12361 gttggaggaa gtggtgttgc acgctggcgt tcttatgtgt ctaaagcatt gaaaatgaat
    12421 ggattaccag ctacagcagc gtatataaat gcctggatgt cccaaatcca aacggaatct
    12481 ggtggtaatg agaaagcaat cggcggcaat gatgggctcg ccgaaggcaa tgcaacagga
    12541 ttacttcaaa cgaaacccgg aacttttgca gctaacgctt ttcctggtca cggaaatatt
    12601 atgaacggat ttgataacat gttagcagca atcaattatg ctaagaaacg ttacggagtt
    12661 gcaggtatgt tgcaagttat cgggaaagga catggatatg ccaatggcgg cttgattacc
    12721 aaagacggac tatatcgagc aggagaagga aataaaccag aaatggttat tccattgaca
    12781 agaaaaacaa gagctatcga acttatgggt caagcattag ccttcctttc tggaagcaat
    12841 aaaaaagaaa cgaaaagtga gattaattct gtagataata gacatatcct acaagcgata
    12901 gaaaaacagt ctcgtatgac aaatgatctg ctatcattat tactctcgtt ttttaaaagt
    12961 aacaatcgat cagataaaga actagcttta gatattcaga aaattttaat caggaggaca
    13021 tagtatttga ggacagtaat attaagaaat aaattgaatg aagaaattga tttgtccaca
    13081 gaagattatt ttgctactga aatgtcgaat ctaggttttg aagttacaaa agagcatatt
    13141 ggacaatggg ggagtttcag agaaatagga gagagtattg aattgtctga atttcaatca
    13201 tcagttatga tttctgttca cggatttaga gaacaacaat tatatggttc attagttgaa
    13261 tttctggcta atggaccttt tgaactagaa ttttcttttg attcagaaac aatgatcagg
    13321 aaatgtagtt tgaaatcatt atctaaaaca gaaattgatt cccaaagctc attgctaaca
    13381 gatactttga atctatattt tacttcaagc tggtattcta ttaagcgtga aaaattaata
    13441 cagaattcaa cttctctcaa tacacgtgga aaagtttact catttgttcg accatatgtc
    13501 tacaccaaaa atgtatggga aaagaaaggt gtatttaagt tcagtaataa ttctgtatat
    13561 ttgacaaata gtacagagcg aatgtctccg ttaaaaataa ggattattgg agagtgctca
    13621 aatccatatt gggaagttat ccaaaactct aaagtagtag cttcagatgg ctattttatt
    13681 aatatgaaag acacacaaat attagaagtg tctagtttgc tagaagacca aacggcaatt
    13741 ttaaaagatg taggtggagt tgaatcttct gtttatcaac aacaagattt tactaaatca
    13801 aacttcgtcc aagcgcctct aggagaattc agcgttgttt ttcatgttgg acatgcaaat
    13861 gtagagattg aactttatga ggagcgtgat ctattttgat tttagccatt accctctttc
    13921 accgagatat gaatttatat aacgaacgtt taatttcgac tgagttcgaa tttggaacgg
    13981 atgaaattgg agaagaagta agtaatttta ttacagataa atatgtccat gtaaaatcag
    14041 gagattttct actagcaaaa tttattccta gcgggaagat tgcttatttt ggagtgatta
    14101 gctcgcaaga agacaaaaaa ataaattgca aaggattact cagtttggcc gatagcgaga
    14161 taccgactgt aaaaatatca ggtgctaatt atgaagagca tattcgacga ctgattgaat
    14221 actacttatt gaacgatcca acaaaacaac taaaaaatat tttggaggta aaaacagaaa
    14281 gcgttacttc tcattcttat caagctgttg acacaaataa gagacgattg aatgcatata
    14341 taataaatgg gttcaaaaaa tataatgtta aatggtatgt caaagagatt aaaggtaaaa
    14401 aaatatatac tggtattcga gcagttgatg attcaattta tattaaagat aattcatctg
    14461 agtttaatga ctgggatgtt tttgtgcaat ctcctggacc agggaacgag aacaaattat
    14521 tgatcgtaga taaagcaatg aaagatatag aaaatccctt ggtattgtct acgtggtatt
    14581 tagatgacga aaataatctt acgcaagatt caacgaataa gaacattgca ttgcctaccg
    14641 ttaatttagt aaatatatat gatcaaactc aagaagatag accttcttat gaagaggtag
    14701 ctaaatcgga gcttaaaggc aacgcctatt cacatgaaat taaagttaat gttgtgctaa
    14761 atgctagaaa tatcaatgta gaaaatatcg aaacaggaat gttagcgact attgtttaca
    14821 aagagcagac ttacaaatct gttctaactg cctggcgaat atccagcagt aaaaagaata
    14881 tagaattgac atttggtaat gtccgaagtc gttttatgga ttattttgaa gataatgggg
    14941 gataaaaaat ggttagcaat gtggatggat atcaatttga aaacgtgaaa gtaagcgcag
    15001 aaaatgatgc tagactttat cacgttttat ataatcggaa aaatcaggtt attgatggtt
    15061 acgatcagtc tatgaattta tcttcaagcg gattaacagt aaaagttgct gcaggagcag
    15121 cgattattca aggtcgtatg gtcgttgttc gacaagaaga aagtataaca gttccagcaa
    15181 actcaagtgg ttatatagca ttaacagtgg atttgacaca agaagttata cctggatcta
    15241 ttcttccaga atcggaagaa tatgaatgga ctaataatca agtcaagcta gaatttataa
    15301 caaaagttat aaaaggtaat ttaaacaatg gtgataaggt ctataattta ccactatgct
    15361 cagttaattc tactggatca actgtttcaa tctcaaagat atcggatagt tacgagctga
    15421 ctctctctaa aggagaaatt ttgtggaggg ggactgcgtt aatgcatgat actcaaactg
    15481 tccaaccttc aaaaaaaatt tggcagacag tttcaggttt tttgttagta tggctcccat
    15541 acgaaaacgg gcaagcaatt gaggatagat atgtaactac gcctttttat aaggagcgta
    15601 taacttttac taatgtttta ggagaaattg tttcaggatt tgatgactat cacaaaaagt
    15661 ggtttagtaa acgaataaac tataattcga atactaatat atttacaggt gcagcaagca
    15721 atgcaagtgg agataacgca aatatggtgc ttaggtatat agtttctttt tagttaggag
    15781 gtatggaaga gtggcaaatt tagaaattaa attatctgca aataagagac agccttatct
    15841 acgtcaccgt gttgttggta gagttggtga tggggggctc acaacaatca atgtacaact
    15901 tcttgaggaa gatgaaatta caccttttgt aattaatcta aacggtactt tgaaatttgt
    15961 gggcgaagtt tcaaacggta actataccga gggagaacca gaaataatcg attcgactaa
    16021 tgggttaatt agttacacgt tcactaagtc aaatttcagc acgagtcatc aattcaaaca
    16081 agcatatttt gaatatgtag atcctaacgg caaaaaagta acttttcaga acttcatcat
    16141 agacgtgtta gaacgagtag atattaattc ggagcaagcg aaatactata tttcttcatt
    16201 ggaaaaatta cagagtgaaa tgcaaaccac tttcaatcag ttcattagtg ataaacaggt
    16261 ccaatacgat caaatctact cgaaatataa cgaattagta agattgataa atgaatcaga
    16321 taagcaagta aatgatcgta ttgataaggc aaataatcgt attgatcaaa ccaatcagca
    16381 aatcggcgat ctcggcaagc tgaaaaagat gtacagtaac agcattga
//
LOCUS       DACDSY010000061        16259 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDSY010000061 DACDSY010000000
VERSION     DACDSY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.061"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(61..1371)
                     /locus_tag="IWK44_001578"
     CDS             complement(61..1371)
                     /locus_tag="IWK44_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9550107.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="IWK44_001579"
     CDS             complement(1383..2801)
                     /locus_tag="IWK44_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9550108.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="IWK44_001580"
     CDS             complement(3041..4609)
                     /locus_tag="IWK44_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP9550109.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="IWK44_001581"
     CDS             complement(4613..4894)
                     /locus_tag="IWK44_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9550110.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="IWK44_001582"
     CDS             complement(4891..5814)
                     /locus_tag="IWK44_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP9550111.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="IWK44_001583"
     CDS             complement(5811..6689)
                     /locus_tag="IWK44_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9550112.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="IWK44_001584"
     CDS             complement(6676..8013)
                     /locus_tag="IWK44_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9550113.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="IWK44_001585"
     CDS             complement(8016..9119)
                     /locus_tag="IWK44_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9550114.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="IWK44_001586"
     CDS             complement(9193..10701)
                     /locus_tag="IWK44_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9550115.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="IWK44_001587"
     CDS             complement(10667..11359)
                     /locus_tag="IWK44_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9550116.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="IWK44_001588"
     CDS             complement(11381..12355)
                     /locus_tag="IWK44_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9550117.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="IWK44_001589"
     CDS             12655..13527
                     /locus_tag="IWK44_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9550118.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..15989)
                     /locus_tag="IWK44_001590"
     CDS             complement(14109..15989)
                     /locus_tag="IWK44_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9550119.1"
                     /translation="MDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNL
                     FWQKKNKGIEMVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFT
                     TQTVKNKEQPTVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMI
                     RNLRIGLVAVSLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQS
                     VSRIIGEKEALSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYT
                     ALVQIDLLEDSHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDA
                     EASKKVMEYCRLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGP
                     SSIIFYQEIVPAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNP
                     LSREVVSVRLINELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLP
                     ITECSQAITDHEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGE
                     KFGDFVQNHRLEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKK
                     YL"
BASE COUNT         4776 a         3426 c         2653 g         5404 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaa
//
LOCUS       DACDSY010000062        16197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDSY010000062 DACDSY010000000
VERSION     DACDSY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.062"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..288
                     /locus_tag="IWK44_001591"
     CDS             <1..288
                     /locus_tag="IWK44_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9550120.1"
                     /translation="DQGWAYQMKAYRNKLKEYKIFQSMSRKGNCLDNSLMENFFGLLK
                     QEIFHGKVYNCFEELKSAIDSYIYYYNNERIKQKLNWQSLVQFRKTTVTVV"
     gene            477..1145
                     /locus_tag="IWK44_001592"
     CDS             477..1145
                     /locus_tag="IWK44_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP9550121.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1345..1731)
                     /locus_tag="IWK44_001593"
     CDS             complement(1345..1731)
                     /locus_tag="IWK44_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550122.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1703..3085)
                     /locus_tag="IWK44_001594"
     CDS             complement(1703..3085)
                     /locus_tag="IWK44_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9550123.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3241..4428)
                     /locus_tag="IWK44_001595"
     CDS             complement(3241..4428)
                     /locus_tag="IWK44_001595"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP9550124.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4435..5466)
                     /locus_tag="IWK44_001596"
     CDS             complement(4435..5466)
                     /locus_tag="IWK44_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9550125.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5482..6936)
                     /locus_tag="IWK44_001597"
     CDS             complement(5482..6936)
                     /locus_tag="IWK44_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9550126.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            7111..8016
                     /locus_tag="IWK44_001598"
     CDS             7111..8016
                     /locus_tag="IWK44_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9550127.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8107..8784
                     /locus_tag="IWK44_001599"
     CDS             8107..8784
                     /locus_tag="IWK44_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9550128.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9065..9961
                     /locus_tag="IWK44_001600"
     CDS             9065..9961
                     /locus_tag="IWK44_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9550129.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10016..11386)
                     /locus_tag="IWK44_001601"
     CDS             complement(10016..11386)
                     /locus_tag="IWK44_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9550130.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11624..12370
                     /locus_tag="IWK44_001602"
     CDS             11624..12370
                     /locus_tag="IWK44_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9550131.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12367..13206
                     /locus_tag="IWK44_001603"
     CDS             12367..13206
                     /locus_tag="IWK44_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9550132.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13207..14157
                     /locus_tag="IWK44_001604"
     CDS             13207..14157
                     /locus_tag="IWK44_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9550133.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14298..15050
                     /locus_tag="IWK44_001605"
     CDS             14298..15050
                     /locus_tag="IWK44_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550134.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15069..>16197
                     /gene="queG"
                     /locus_tag="IWK44_001606"
     CDS             15069..>16197
                     /gene="queG"
                     /locus_tag="IWK44_001606"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP9550135.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQK"
BASE COUNT         5265 a         3033 c         3262 g         4637 t
ORIGIN      
        1 gatcaaggtt gggcgtatca aatgaaagct tatagaaaca aattgaagga gtacaaaatc
       61 tttcaaagca tgtctcggaa agggaactgc ttagataatt cacttatgga gaattttttt
      121 ggcttattaa aacaagagat ttttcacggg aaagtttata actgtttcga ggaattaaaa
      181 tcagccatcg atagctatat ctattactac aataatgaac ggataaaaca gaaattaaac
      241 tggcaaagtc tcgtccaatt tcgtaaaaca accgtaacgg ttgtgtaaaa atatcatttt
      301 agagcataaa aaaaaggagt ggatttctcc actccaaaaa agtctaactt ttgggggtca
      361 ctacagtgtc acagtccttt ttttagtaaa aggatacatt tcttccttgt atattccatg
      421 cgtgttcgtg agaaagcata tataatttaa ttaacagata gttaggggga gaaaaaatga
      481 ttttttcaat tacaatgaaa ctggtgttcg ggttgatcgg tttattatta gtggtacgtt
      541 tgcttgggaa gaaatccatg tcagaaatca caccgtttga tttagtctat acgcttgttt
      601 taggtgggat cttagaagaa tcaacctatg atgacaatgt ccatgtaggg catgtcctat
      661 ttgccattgc gctttgggca gtaatgattt atggtatcga acgtatcgtc caaaaaaatg
      721 aaaaggtcaa tcgttgggtc aaaggcgagc catctgttct aatcaaagac ggcgtcatca
      781 acatgacaga gttgacaaat aaccatatcg agatggagca gttgcgagca atcttaagac
      841 agcaagaatg cttttctctt gaaaatgcca aacatgttat cctagagaat gctggtcaaa
      901 tgagtgtatt gaaaaaatca gatgaagaca aagctttatc gatcctgctt gtagatgaag
      961 ggcaaattca gcataaagtg cttcagtcca atcaattgac tgaagcatgg cttatggaga
     1021 acttgaagaa agaaggctat gcagatgtaa aacagctgat ttatgttgaa tggtcagaag
     1081 aaaaagggct ctacatacta aataaagaag acgtcatgca tgaatcttac cgaatagatg
     1141 gatagataga cagaggttgt gaaatcagcg ccttggcttg ttactcaaaa ctaagcgact
     1201 ttttcacaac ctctgatatc taatatacgt tgttccagaa gttgcttctt cgaaaaataa
     1261 gccaaaattc cacaaaaatt taagaagcaa ttttcgaaaa atcctttctt attttttgaa
     1321 gttgatcact tctgtcacaa cctctcatgt cacaaaccat ttttttgttt cctttaactc
     1381 aatataccgt acgtcttccc acaatatcgg ctgatggtca atcataaagt aatcttcata
     1441 ggcttcacca atgaagtgcc cttcgacctc atccaagagt cttccaaatt catcttttaa
     1501 attgagctga atgattactg ctttacgaga taaaaaggct tctgtcagta tactatcgat
     1561 ttcttgcaat gtcatttgtt gtttaggaag aggctgtttg cgtgcttctt cttcattttt
     1621 tttgataccg ttgactagtt cttccatcgc aaaagcagtc ccccatttta atccaaatgg
     1681 ccgatctcga tactcgttat agtcaatgaa tttttttgct gtccgtctca tcttttccac
     1741 cttctaatcc actcatacca cctgcatgcc cgccaacgag agaactacgt gcgatggcgc
     1801 gtcctccttg caagagacta tgcgcatgga tgattgattg aaagccgtat ttttttctaa
     1861 tcgtatcgac aacaaaatca actttcgctt catttatctg cttttcagga tcttcaaaaa
     1921 ggttaagctg taaacctgtc gcgtatacga ggtcggaaca gttcacgcct acacgccgaa
     1981 tatcctggta gtggtaatgc tggtggaaaa tggtcaataa agcatctgct agatctctgc
     2041 tagcatttgt cgcttcgatt ttcatttgct ggtgaaaccc ggtcttgcca tttggatcaa
     2101 gatagcctaa agagtatcct acccataagc tgactacttg tgtttttgct gcagatcgtc
     2161 tcaagcgagt gccgacttgg tcggccattt ctttgataac gatctcgatt tcttctcgtc
     2221 ttgtataatc tctatctagg atttgactat ttccgatact ttttgatttt ggtgtatatt
     2281 cttgtcccaa aaaacttcga tcgatgcccc aagcatgggc ataaagctga gtaccgatga
     2341 cccctagctt gcttttcaag tgataataat ccgcgtgagc taaatcataa acagaatgta
     2401 tacccatttg tttcagacgt tttgctgttc gtttgccgat tccccaaaag tcagtgatat
     2461 ctgagatttt ccataacttt tctggtacat cttcataacg ccattcagca aacatttgct
     2521 ttccgtcttt tgagccattg tctaaggcta gtttagcaag tagcgggtta tcgccaattc
     2581 ctatagtcgt gtagattccg gtctgctcaa acacatcttt atggatcagt ttagccatgt
     2641 cgtacgcatt tgttttacca aagagtgcca tgctatttgt tacatcaagg aagctttcat
     2701 cgacggaata gacatgatgg ttcgcttcgt ctgcatattt tttatagata gaattgattt
     2761 ctctgttctt ttccatataa taggccatcc ttggggcaac aatatataaa tcacttggat
     2821 aggggaaggg aagatctctg gcacggctga tattggtgat gccgtaagct ttttttgctg
     2881 caggactgga agccaagatt aatccgctac cccgtgtttc tgtcgaatcg gacggatagg
     2941 acatgactac taatttagtt gtaaggggat caagatcctt ttcaacgcac tctacggagg
     3001 cgtaaaaaga tttgcagtca atacataaaa tatctcttga aggttctttt tcataaacaa
     3061 ataccggatt ttgattcgcc cacataacaa tcacctcttc aacacaatta gaacacacgt
     3121 tctctttccg tgtcaataaa aaacagcaat tttcaaatac gagcaaaagg ctcacatttg
     3181 gaaattgctg agaggttatt tttatctatt tgtttttagc tgagatcgtt gattttttta
     3241 tcaaacatga acagcttctg gatcgatctt tgttgtccca tttccaatgt gtgcaacata
     3301 aaattccgga gcatagccga caactttcat gtattcatca tagacattgt ttttgaaggc
     3361 aggtatattt tcttctttga caagagcaat cgcacagcca ccaaatccag ctcctgtcat
     3421 tcgtgcacct aagacacctt cttgtttttg agcagcatct actagtgtat ctaattcgat
     3481 cccagttact tcataatcat agcgaagaga gtggtgagaa tcatttaaca gttgaccaaa
     3541 tgcttctaag ttcccagctt ctaattcagc ttttgccttc aaagtccgct gattttcaga
     3601 aacggcatgg cgagctctgc gtatcaatgt atcatcgccg atttggtcaa tagttgattc
     3661 aaattcttct tcatctaagt ctcctaaaga agagataggc aaagtttttt gcaaacgtgc
     3721 caaggcttct tcgcattcgc tacggcgttc attgtatttt gaatctgcca attcccgccg
     3781 cttattcgta ttcatgatga cgattgcata accatctagt ttcactggaa ccatttcgta
     3841 tttcagcgta tttgtatcta ataagattgc atgatctagc tcaccgaagc cgatggcaaa
     3901 ctgatccatg atacctgaat tgacaccgat gaattcattt tctacttttt tacctgtttg
     3961 gactagttcc aagcgatctg tttttaaatc aaacagatct tctaatacaa cacctactaa
     4021 taattctaat gaagcgctac tggaaagtcc tgcaccatta gggattgttc cttccactaa
     4081 caactcaaac ccatgatcga ttgagtgtcc agcttctttt aatttaagga ccattccttt
     4141 tacgtaattt gcccagtttg cttttttatc atattctagc tcatctaaag taaaactaat
     4201 gacaccgata tcgtcaaagt ttgtggaata aactaatatt ttattatcgt ctcgaggtgc
     4261 tgctacacct tgcgttccat aagtgatgga agcggggaag acatggcctc cattgtaatc
     4321 tgtgtgttca ccaatcaaat tgatgcgtcc tggtgagaag tattgtgtag tttttttagg
     4381 tccgaatgtt tcggcaaatt ttgtgtttaa gcttcttttg atttccatga tgagttattc
     4441 ctttcttgtt ttgattttga attctgtcac tgtttcaaat actgatccag cttttaaatg
     4501 aatcgaacca aatgctgaga agcgctctgc acctggagct gtttgagtct caaatgtgat
     4561 gccaccgtga tgtgccagtt ttcggttgcg catttctggt gtttctgtgc cgaaatttgc
     4621 agtgaaaata accacactag aagcatctgt agcaatacct acttggattc ttttatcggg
     4681 gctagtcaag cgagcggctt ctttacccaa gccggtttct tttaagaaaa atggatgatc
     4741 gattccgtca acaagatttt tttgatccaa atcactagca aaaacggacg ctaatttttt
     4801 aggtatttga aaatcgaaag cacttcctgc agcattttct tttaccccta taggaatact
     4861 atctgttcgc aaaggagcat aagcttcgct attgagccaa agctcatgtt gatcgattga
     4921 ctgagaagca tctcccgtca agttgaagta aacatgattt gtcggattga ataaagtatc
     4981 ttggtcactg gtgccgcgag aggtcactcg ccagatatta tcttttgtca aagtatagcg
     5041 gatttccaca tccattgtac ctggaaaacc atgttctccg tcgggacttg tagtagtaaa
     5101 aatgacagaa gcttcatttt cgccattcaa aatgacataa gaccattttt ttaattcaaa
     5161 tccaggagca ccaccatgca gattatgtcc agtttttgga tctgtggcta attggtaagt
     5221 ttttccattg agagaaaagc ggccattttc gattctccct gcggtgcgac cgatagaagc
     5281 accgatatac ggatcttttt cgatatattc ttcagcggtg tcaaatccca aaactaattc
     5341 tcgatcattt gattttaaac tgacgattct agcacctaaa tcagagatag aaagtgctag
     5401 cttgtttttg ttctccaagg ttatcaagtg ataaccttgg ccaaaatctt tttctgtaat
     5461 cttcattttc aagatccttt cttaatggga aggaactact gcatcgtgaa gttcatcttc
     5521 cattgtatca gctggagcca atttgttttc caattcctca acgatttgtg catgtttttt
     5581 ctctgtcaat gtaatcttcc aagcaaaaat aatagcggag aaaatcatca aagcagcagg
     5641 agcatagaat gcataagctt tgaaagtcgt tacgccatga gcagtgatat cagcagcagt
     5701 agcattacca gtcatcccag aagcgatagc gacaaaccct acgatcccgt tagacaaagc
     5761 accagcgatt ttatctagta aagggcgaat tgatagagta acagcttcgt tacggatacc
     5821 atttttccat tgaccatatt caacagagtc tgtgatcgtc atcaaagcgg ataagaaaat
     5881 caattgttga gggaagtaga acaatactag cccgatgata acgattggca aacttgaacc
     5941 tgcgatcgta aagaaaaagt aagaagaaat catcattgcg atccctccaa ggtaaacgta
     6001 gcgacgagag atgacgcgtg taagcattgg gaatagtgga acagctacta aaccaagtaa
     6061 ggcagcaact agaccaacga tccaaaattc agttggttgg ccaagtacat atttgaagaa
     6121 gtagaataat acggcagttg ttgctacatt ggcaatcgca taagcgatat aagcaagggc
     6181 aagccacatc aactgatcat tttgagcaac tgctttgaac acatctttga tcccagcttt
     6241 ttcattttgc tgacggatcg cagtatcgac ttcttttgtg cccaaggcag tgataatcgc
     6301 agtaccgcct gaaataacag cagcaatcac accgaaccag aaccagcctg aagcgccttc
     6361 aatatgttta tgagtaacaa gataggtaaa gaaagtaacg atagggacaa cgactagagt
     6421 agtaccattt gcacctagag aagagccgaa acgagcaaac gtagcaaatt tttcacgttc
     6481 ctttgtttcc gtactcaaag ctggaatcat tgaccaaaaa gcgatatcct taaatgaata
     6541 aaaactatcc aataagatga aaacgattgc aaataatatg ataaataaag tcgtattatg
     6601 agtagctaag ccaaagaaat tggtaaagat tgctaccaag agtacggaac taacaacgcc
     6661 gccgataact aaccatggtt tgaatttacc gtattttgta cgagtattat cgatgatgcc
     6721 accaatcaaa ggatcaaaaa taatttcaac taagcggata cctacaacta agcttgtaac
     6781 tgtagcaatc atttttgcat tcgttgcctt atctgttcct gcgaacatct ggcttgtcac
     6841 gaataccata aaataagtgc ttaatgtaac atagaatgtg tcgtgtccaa aagcgcctaa
     6901 agcgtaggac acacgttgga tcattttgct tttcataatc tgtattcctc tcctgttcaa
     6961 tatataatac taagtaaacc ggttacaacg aataacaaag atcttttaat gacttaaact
     7021 taacaagaaa tcgcttcctt tttaatatat ttctgtgact tttatttaac tttttgttgc
     7081 attatactaa aggagtaagg agggatagac gtggagcaat cgattttttt aaaaaaggaa
     7141 catcaacaag tagaaaaaga attgtatttt gatttttgtg gtttctctaa aacgatgccg
     7201 tttcattctt ttggtcctgc tattcgcaat gattacatcc tgcatgtcgt gatggaaggc
     7261 aaaggatctt atcatgtgaa aaatcaacaa ttccaattga aaaaaggtga tctgttcttg
     7321 attcgtccag gagactcgac gttttactta tcagatggag aagatccgtg gatgtattgc
     7381 tggctttcat ttggtgggag tgctgcagaa gaaatcattc ggcagtcgct tttcagagaa
     7441 gaccaataca cgatggtctc ttctgacata caaaaatata tcgacattat tttagaatgc
     7501 atgaaagata cggaagaaac cttggaaaat gaattacagt taacagcatt aacctaccgt
     7561 tttttatcag tccttttaaa agatggcgga aaggtcagcg gtggtgtaca aaaggttcat
     7621 tcctctttag ctgttgaaac agtcaaatac atcgaggcac attatgctga aaaattgtct
     7681 gttgaggaaa tcgcccagca tttagcggtc aatcggagcc atttgtcgcg agtattcaaa
     7741 aatcatatgg gtaccagtat caaggaatat ttgatcgggg tacggatcaa tcgaggggcg
     7801 tttcttcttt ctctgacgga tgaatccgtg gaaagtattg cctatcaagt agggttcaat
     7861 agtctagtcg tatttagccg aatgttcaaa aagagtaccg gagaaacggc tacgaactat
     7921 cgtaaacgta tgaaaaacga aaattttaag aatattgctt tggatacctt gaaaaaacag
     7981 ttggaaaaac aaaccgcagt ttcttggaca acctgattag tctggttaaa atgacaagca
     8041 tcggcaaaag aaacttgtgg cggttatgct acactgtaaa gcgaagacta aaaaggagga
     8101 aaagggatgc gtacacttat catcattagc catccaacga tcgaagaatc tttaaatcag
     8161 caatttttca aagaagcttc tgctggttta ccagctactt ggcatcattt ggaatcaagt
     8221 tatccagacg gaaaaataga tatagagact gaactacagc tcttggccca gcatgatcgg
     8281 atcattttcc agtttccgtt ttactggtat agttctcctg ctcatttaaa aatttggcaa
     8341 gataatgtct tggaacgagc agcacatgta ttaaaaggga aagaactagg aatcgtattg
     8401 acaacaggtg tggcggaaaa agaataccaa gctggaggaa aagaagaata tacgatttcc
     8461 gaatttttga ggccgtatca gcgaatcgca aataaattcc atatgaccta tcttccgcca
     8521 ttcgttttag cacaatttat gtatttatcc caggaaaaac gttgggagaa gctaattgct
     8581 tatcagcagt atctctcatt ggaaggcaaa ccatcgctga ctcagcggat tgactggttt
     8641 attcaaagag tacaggaaaa tcagaaaatg caggaagaag aatctgaaaa acaagcatat
     8701 atcgtagaag cactgactga ggcaaaagag cagatcgaag acttgacctt tacactgcaa
     8761 gaaatgaaag gaacttcatt atgacaaatg attggctgat ccaacaatta gatgaattaa
     8821 gcgaaaaatc agaatatcaa gaccgtgcgc tcttatatga aataaagaaa ctgatggctg
     8881 agatagacga acgaacaatg cagctgcaag gagaaatcga cggacaatct tggaatcatc
     8941 tcaactggtg aaattttctt ataaataaca aagataaaaa tgtgcacagc gacaattatt
     9001 tgctacaata agaatatgaa gcgtttgata acgctattat acataaaaat tggtggtgaa
     9061 aaaaatgtat gatgaaaatg agtggggaga aaatgttcgg atgacccgaa gaagacagca
     9121 aaaacaggca gctcgtaagc ggatgatcat gattggggca gcagttgccg tgtttttgat
     9181 catagcagga ggagttgcgg gtctatattt tcatggaaaa aattcgtcag aagcgaaaag
     9241 tacgaccata acggaaacaa cgacaaagaa aacagagacg aaacaaacaa gtgagtcatc
     9301 aactaaaaca aaagagacac aaacttctga agaaaaaaca gcaaatggtt tgccagagca
     9361 acttcaagca atcgtaaatg attattcgaa aaagatcgaa gaaaagacgc ctgcattgat
     9421 tgaagaatac cagacagaaa ttcagggaaa tcaagaggga atagctggtt tatcagcagt
     9481 tgctaaccaa aaagcacgtg agcttcaagc aatctccgac gaagggatca gaaaattacg
     9541 tgcagcgtat caggcagcag aaaataaaga cggtgtagac ttagatactt tgatcaatca
     9601 attatcggct aattatacga atcatgtcgc taagatttcc gatatttatt tgcagactag
     9661 cgcctctctt caagcagagt caacaagcac acaagacaca acatcttctg attctgagac
     9721 agcagagtcg gtagaagctt ctgaaatgca agctcgaaca acacaagaca caacagattc
     9781 ctctgaaact aataccaaca gcgcgacttc acaggctgcg acaaccgtag ttttggaagg
     9841 agaaggtcca aatcaaatag ccgaacgaac tggcgtacca gtagaaacga tcttgtcgct
     9901 gaatgggatg agcatggaga attatttcct gactcccggt gagacgctaa aattgaattg
     9961 ataaaagcaa taaaaaagaa cgaagcttcc actaaggtga agcttcgttc tttttttatt
    10021 ttccggttct tttttcgcgt acaaagtaat ggatcgtcat gtaaagcaag aaaacgagtc
    10081 caataatcaa tgagattctc gtttcaggat tgataaacat gaaaatcaat acgatagcta
    10141 aaaaagcaag tgtcacataa ttcgtataag gagatagtgg cattttaaaa ggatgatcat
    10201 gcattttatc tgcgtgcttc tttttgaagc gaatctcgct gatcaaaatc actacccaag
    10261 gaaccatacc aggcaaaata cttgagctgt atacgaaaac gaaaatattt tctgactggt
    10321 tgatgaacat tggtagaaga taatttaaga tcagaccaat taagattcca cctgagattg
    10381 ctacgacagg ataaaaaggc gcaccgtgac gagatagttt cagaaatttt ttagacatct
    10441 ttccatttat ggcaagtgta tagaccatac gacttgcgct gaaaatccct gaattgcaac
    10501 ctgataatgc cgctgtaatc acgacaaaat taatcagtcc tgcagcaaat gtaatgccga
    10561 ctttagaaaa agtttgtaca aaaggtgaac caatctcact caattgattc cacggataga
    10621 tactcacaat gataaaaata gcaccaatat aaaaaatcaa gattcgaccg atagtggact
    10681 ggacagcttc aatgatcgtc tttttaggat tctctgcttc accagcagtc attccgatca
    10741 gttcgattcc ttgataggaa gcaacaacga tcgataatgc aaagaaaaat cctttgacgc
    10801 ctccagtgaa gaatccacca tttttccaaa gatttgaaat ccctactgcc tgaccatgat
    10861 taccaaatcc aaataagatt accccaagac ctgcaatgat catcaaaatg atcgttatga
    10921 ctttgatgat cgaaaaccaa aattctaatt cgccaaacgc cttgacagaa ataagatttg
    10981 ccagcataag aaacaagacg acaatgattc cgggaataat tgccggcaaa tcaggccacc
    11041 aataattcat ataagatcct acagcaatca cttcacttat tcctacgaca atatattgaa
    11101 aaatattact ccaagcggtc aagtagccag ccacaggatg gatgtactcg ctcgcgtaat
    11161 ttgcaaaaga accagtcgaa ggatcaacgt aaagcatttc gcctaaagca cgcataatca
    11221 aatacaaaaa gaatcctgca atcgcatagg ctcccatcac tgagggccct gtccaacgta
    11281 tggttgagga ggcacccata aacagaccga caccgatcgt tccacctagt gcgatcattt
    11341 ggatatgacg ggcactaaga cttcgagtta acttttcttc ttccatacaa aaactccttt
    11401 cgttgttgag tcaacaagaa aaaataatca aacgtttcaa cacatgtttt tcattattag
    11461 gttagcctta ttgaactctt gatgtgattc tacagaaaat tttcataaaa tgcgaggaaa
    11521 attcgaaaaa aactttttca tcattctact tgaaaatgct ttccatttta gttaaactat
    11581 atttaaataa aaagttaggt aaacctaaaa aggagtgtaa gatttggaac gtacggccat
    11641 tgtaattcaa gacctttcag ccgcttatca agggaaaact gttttgaaaa ctatcaactt
    11701 gacgataaat ccacaaaaaa ttaccggtat tatcggacca aacggtgcag gaaaatcaac
    11761 atttatgaaa tctttgctag agttgatacc agctactact ggtaaagtca ccttttctgg
    11821 acagccggta aactcagtcc gtaaaaaaat agcctacgtg gaacagcgaa gtgaattgga
    11881 tctttccttt ccaattgatg tattaggcgt tgtactttta ggaacatatc catctttacg
    11941 aattggacaa agacctggga aaaaagaaaa agaacgtgca agacaagctt tgaaaaaagt
    12001 agggatggaa gaatatgcca aaagacagat cagcgaacta tcgggtggac agcttcagag
    12061 agtttttatt gcaagagctc tagcccaagg agcagaatgg atctttttag atgaaccatt
    12121 cgtagggatt gatgcgttaa gtgaacgaaa gatctttgac atcttgcagg aattgaagaa
    12181 ttcaggaaaa acgattttga tcgtccatca tgatcttcat aaagtagaag aatatttcga
    12241 tgaggttatt cttttaaata aacagctgat cgcttccggt ccagtacaag aagtctttac
    12301 atcgaagaac cttcaattgg cctatggtga gatcatcaga catttagtaa aaggaggaga
    12361 gaaaaaatga tccaatcctt tattgatgga ttaatgaatt atcaattcct gcaatacgct
    12421 ttggtcactt ccatgcttgt aggacttgct tctggagtca tcggttcttt tattatttta
    12481 cgagggatgt ctttgatggg agacgctatc tctcacgcag ttcttccagg tgtagccatt
    12541 tcttatatgt ttggttttag ttacatcttt ggagccactg cttttgggat gctaacagca
    12601 gcagtgattg gtttcgtgac tcagcggagc cgattgaaaa atgatacagc gattggaatt
    12661 gtctttagct ccttttttgc attagggatt atcttgattt catatgccca gagtgcgaca
    12721 gatttgtatc atattttatt tgggaatgtc ttagctgtta gggaaagcga tttgttgctg
    12781 acagcactcg ttagtggaat cgtcttgata tttgtctttt tcttttataa agaattgaag
    12841 atcacatcat tcgatccgac aatggcaaaa gcttatgggc tgaacacatc gctgatccat
    12901 tatcttttga tgttcttttt gacattagtg gctgtagtca gtttacagac agtaggaaca
    12961 atcttggtga ttgccatgtt gatcacacca gccgccacgg cttacttgct aacgaaccat
    13021 ttactgaaaa tgatcattac agctgcagga atcggtatgc taagtgcagt tgtcggtgtg
    13081 tttttcagtt atagttacaa ttggccatca ggagctacga tcgtgttagc atgtaccgca
    13141 ttttttatcc ttgctttttt attttctcca acaaaaggaa ttttatttca gaaaggaagc
    13201 agatagatga aaacaaaaaa atcactcttt ttaatcttgg cagttagttt cttggtatta
    13261 gccggttgcg gaaagcaggc ttctgaccaa gcggatgaag gaagtaaaga aaagttgtca
    13321 gtcgtggcta ccaattcgat cttggcggac atggcaaaag aggtcggaac agatcaaata
    13381 gatatccaca gtatcgtacc agtcggaaca gatccgcatg aatatgaagt actaccagaa
    13441 gacatcaaaa aggcaagcga tgcagatgtt attttataca acggtttgaa tcttgaaaca
    13501 ggtaacagct ggttcgataa cttgatggaa acggctaaaa aagaagaagg aaaagattat
    13561 tttgcagtta gcaaaaatgt agaacctctt tatttaacta gcggtgaaga acatacaaaa
    13621 gcagatcccc atgcttggct agacctatct aacggaataa aatatgtgga ggaaatcgca
    13681 cgtatattct ctgaaaaaga tgcagaaaat gcgacactct ataaaaaaaa tgcagaagca
    13741 tatgtggaaa aactaaaaga attagataca caagcgaagg aaagttttgc ttctatcgaa
    13801 gagaacaaaa aattattagt aacaagtgaa ggggcattca agtatttttc acgagcatat
    13861 gatttgccag cagcttatat atgggagatc aatacagaaa gtcaaggtac gcctgatcaa
    13921 atgaaagcga tcattgatca gataagagca aaagaagtac cggttttatt cgtggaaacc
    13981 agtgtagact caagaagcat ggaacgggta gcaaaagaaa cgggtttgaa aatctatgat
    14041 aaactgttca ctgattccat agcaaaagaa ggagaacaag gagattctta ctatcagatg
    14101 atgaaatgga atattgaaac gatccatgaa ggtttgtcac aaacaaaaga aagctgaaaa
    14161 gtgcaaggtc tagcttgagg agcagacctt gcatttttta taagagatat gaaaaaaaat
    14221 tatgctgttt ttcgtcgtac agataccttt tttgaagaaa ttccgttata gttagagtaa
    14281 ggaaggaagg tgtggctatg aattttacac gtaaagataa atatacgatt aaagaagaag
    14341 tctttcgatt gatcagccag caggaaaata ccgacaagga tcaagacctt caatttcttg
    14401 aagcagaatt agctaaatta ttcatagcaa atcaaaatga gacaacagaa ccagatgaag
    14461 agctgtacat aggcaaatgg ttgtttgatg aatttcagcc tttagatgaa aataccggta
    14521 tcggttattt tgtgttcctt tctccagtcg gcttcacacc tggcgtatta gttaaacaaa
    14581 acgacgaaat aaccgtcaaa gcatggcctg cattattttc agtcatcaaa gcagaaggag
    14641 taatcaaaaa gatccagtcg gcctattata aaagtcaatt tattgccaac agcaaattaa
    14701 tgaatatgtt ttataatcgt ctagtagaaa ttgctaggcc tgggaaaatc ccgcaaacat
    14761 tcacgtacac acagcaaaaa aatctctatg aagaagtatt gtattcccgc gatctgtctg
    14821 agttttcgcc atgggaagag gtaaggctcc atgaactgtt aagtacaaaa ccagtttcat
    14881 tgaatttttt taatgcatta gataacaaaa gaaaattaat ttcaacaaat tattgggaag
    14941 tatttgctgc aagaaaaaaa tggatggaag aaaagaagat tgccgatcag cagatccagt
    15001 ttgtccagct gaaaaaagaa aaactatatt atgggttata cagccgttaa cagcaagcga
    15061 ggaagaccat gacattaaaa gaagaaatca tacaagaaag caaacgatta ggaattgaca
    15121 aaatcgggtt caccacggcg gaaccttttg attcattaaa agaatcgttg gaagaacaaa
    15181 aagcagctgg ccatacttct ggctttgaac atccaaatat cgatgagcgt ctttatccag
    15241 aaatgacatt tgatcagccg aaatcgatca tttccattgc acttgcgtat cctactagga
    15301 tccatgaaga agtgcctagg gatgaaaaac gcgggcagtt tgctcgtgct tcttggggga
    15361 tcgattacca tgatatactc agagaccgtt tgaaccgatt gatcgaattc atccaaacaa
    15421 gagcagaaaa atggcaaaaa gaagaggaat ggcgtctagc tccgcaagta gatactggag
    15481 aactgatcga tgtcgctgta gcacagcgag ctggtttagg atttattgga cgaaacggat
    15541 tgttgatcac tgaagagttc ggttcatttg tctatcttgg tgagatcgtt actaatattg
    15601 tatttgagcc tgataaacca ggagaatttg gttgcggtga ctgtatgcgc tgcgtgactg
    15661 cttgtccgac aaaagcattg cttggtgacg gaagaatgaa tgcccagcga tgtttgtctt
    15721 atcaaactca gacaaaaggg atgatgccgg aagaatatcg taaaaaaatg cgaaatgtca
    15781 tttatggatg tgatatctgc cagttagtct gcccttacaa tcaaggaaaa gattttcatt
    15841 ttcatgaaga gatggaacca aaagtagacg aagtatatcc aaaattaaag ccaatgcttt
    15901 cgatgtctaa taaagacttc aaacagcaat ttgggcattt agctggttca tggcgtggga
    15961 agaaaccttt gcagagaaac gcattgattg ctttagctaa tctaggtgat cgaacggcat
    16021 tgcctgagat ttcagaatgt ttaacagatg tccgccctgt catcagagga acagcagctt
    16081 gggctattgg acgtctagga acaaaagaac cggaaaaatg gcttgatgta ttagatgaaa
    16141 tattagctaa agaaacagaa gaagtcgtta taaacgaatt ggaacatgcc cagaaat
//
LOCUS       DACDSY010000063        16098 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDSY010000063 DACDSY010000000
VERSION     DACDSY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.063"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            22..1449
                     /locus_tag="IWK44_001607"
     CDS             22..1449
                     /locus_tag="IWK44_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9550136.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1541..1906)
                     /locus_tag="IWK44_001608"
     CDS             complement(1541..1906)
                     /locus_tag="IWK44_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9550137.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1903..2268)
                     /locus_tag="IWK44_001609"
     CDS             complement(1903..2268)
                     /locus_tag="IWK44_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9550138.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2460..2708)
                     /locus_tag="IWK44_001610"
     CDS             complement(2460..2708)
                     /locus_tag="IWK44_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550139.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2707..2835
                     /locus_tag="IWK44_001611"
     CDS             2707..2835
                     /locus_tag="IWK44_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP9550140.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2892..4586
                     /locus_tag="IWK44_001612"
     CDS             2892..4586
                     /locus_tag="IWK44_001612"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP9550141.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4653..4859
                     /locus_tag="IWK44_001613"
     CDS             4653..4859
                     /locus_tag="IWK44_001613"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550142.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5237..6370
                     /locus_tag="IWK44_001614"
     CDS             5237..6370
                     /locus_tag="IWK44_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP9550143.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6401..6721
                     /locus_tag="IWK44_001615"
     CDS             6401..6721
                     /locus_tag="IWK44_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550144.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6750..7214)
                     /locus_tag="IWK44_001616"
     CDS             complement(6750..7214)
                     /locus_tag="IWK44_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9550145.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7346..8599)
                     /locus_tag="IWK44_001617"
     CDS             complement(7346..8599)
                     /locus_tag="IWK44_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9550146.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8628..8945)
                     /locus_tag="IWK44_001618"
     CDS             complement(8628..8945)
                     /locus_tag="IWK44_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9550147.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(8971..9291)
                     /locus_tag="IWK44_001619"
     CDS             complement(8971..9291)
                     /locus_tag="IWK44_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9550148.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9755..11170)
                     /locus_tag="IWK44_001620"
     CDS             complement(9755..11170)
                     /locus_tag="IWK44_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9550149.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11187..12503)
                     /locus_tag="IWK44_001621"
     CDS             complement(11187..12503)
                     /locus_tag="IWK44_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9550150.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12653..13351)
                     /locus_tag="IWK44_001622"
     CDS             complement(12653..13351)
                     /locus_tag="IWK44_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP9550151.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13640..14629)
                     /locus_tag="IWK44_001623"
     CDS             complement(13640..14629)
                     /locus_tag="IWK44_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP9550152.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14709..15542)
                     /gene="msrA"
                     /locus_tag="IWK44_001624"
     CDS             complement(14709..15542)
                     /gene="msrA"
                     /locus_tag="IWK44_001624"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9550153.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15717..>16098)
                     /locus_tag="IWK44_001625"
     CDS             complement(15717..>16098)
                     /locus_tag="IWK44_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550154.1"
                     /translation="YFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYR
                     VTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVT
                     TIISYLVTLTAYLCLFFKIVFHKR"
BASE COUNT         5009 a         3250 c         2742 g         5097 t
ORIGIN      
        1 atacaaggcg gaggctctac tatgattgaa gaatatattg agaaaaatat tttaagacag
       61 ctgtttttat gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa gctctccaat
      121 ctcctgcatg tttgcaaaac cactttactc aatgatatca ataacataaa aaaagaattt
      181 gaagaacaga tcgcttatac acatcgggaa aaggaccgtt acactttata tttttcagaa
      241 catatacctc gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact
      301 tgtctgcttt atttggaaga agatgaacct gattatcttc aattaactga atgtgaattc
      361 atctctgtca gcaaagctta ttcattaaaa aaacaagtat tagcttactt taatgactgc
      421 gggatcgaaa tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg tctgctgctt
      481 cttaatgtgt cgtatcgttt aggcggtttt aattcttggg agctacctga atcgtttttt
      541 gaacgtgccg atcgtttcat tgaatccgta acggaaaata gtggcagatt ttatgataaa
      601 gaaaataaag aaatcttatc gattggtttt gctatcagtt tcctacgcca gcaagtgtgt
      661 gctgtgacaa tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat ttacaattat
      721 gtcgaatcag catgggaaaa cactgatttc caaacctatt ataaaaaaga agaattcgcc
      781 tttattttga cactattcaa tctttgtaat tatggtttcc attcttatca gttgattgcg
      841 gaagactttc aacagctaca ccaagtgttt attgacaata ctcccgaaat aaaagaacta
      901 gttgctacct ttgaatctca cttcaatcaa gaattattcg gaaaccaacc gttcgaacgt
      961 gcgctgattc atctgatgcg ttcggcttgg gataactatc aattattcat gccagaaaaa
     1021 ttttatcttt taaatgagga acagacaaat ttatataaag aagtccagac aattttttcc
     1081 agctggtcca gccagttacc ttatgatcta cggttgaatc ctaattgcat gcgggctttt
     1141 gtgatcgaat tgagcggaat tctccgtctg acaaaagaac atctgactat ctatatcgtc
     1201 acaaattcgg atgttcatta tctgatttat cgggaagcct tggaagctgt aactactttc
     1261 gattttcaag ttgcaccaac aatttattca agtatatctg atataaagaa gtatgcgcag
     1321 cagtcctcaa accgggtatt atgtgaacgt acattatata caccagatgc ggtccaatat
     1381 gaaaatatta ttcctatttc gatcaatacg atcgatcgag ctatcatttc agctgtgcaa
     1441 aacaaataag gggctgtgac aaaaatcatg tcacagcctc ttatttgttg aagatgccca
     1501 tttctgtaac aatctcttga tacacgtcag gctcacaacc tcacttattt tggacatcta
     1561 ggggtccgat ctctttattt aaaaaataag aaatgatggt tcggataact accaacgccg
     1621 ctagttggaa aatatcctgc aaggtaggtt tgataatcga ttcgatgatc ccagctgaaa
     1681 caagaatttc caaacttaga agtatatagc ctcccaacat ctttttaata gcattgttct
     1741 cgcttactcg gtcgctttgc acttttgtat ggacaaataa atatttcaaa aaatccttga
     1801 ttgatagaca aacaccccaa accaataaac agatagcaaa gatatcaagt cccagtacaa
     1861 gtttttcgaa atatggaaga agaaactgca tcaactctgt cgtcatccta cctctcctct
     1921 tctttgtttt tctttgtctt ctatttcctg attcaagaaa taagaaatga ctgttcgaat
     1981 gataataata agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat
     2041 gatatcagca acgactagta cttccaagct taacaaaata taacttgcta gcagttttct
     2101 tgtatctgca ttacgagtat tacgttctgc atagtttttc ttgacatgag accaatcaaa
     2161 aagtttgaat agaacaagaa cgatcccgac aattaagata ccgatagaac aaatatttaa
     2221 aatagcaatg atccattcta gaaattcaat taaatttgag cgaaacatat acgctaccta
     2281 ctttctttta atttgttcct attcttttat tgtaaaagaa agttaccttg ttcgtctatt
     2341 taaaagttgt tctaatgata acggttcgct taaatttttc tttttcactc tcccattttt
     2401 ctccctccat ccataaatcg tggaagtatc caaaacaacg tagcaagtta aaataaggct
     2461 tagtccgtca tcagcttttc aaagaagcac tgtctcggtg ctttttttgt acccaaattt
     2521 ctaaaaatag aaccatacac ctcctcaaat cttttcttct tacctaccct atttttattt
     2581 acaggaaaat cagacacttc ttcttttttg tcaaaatttc ctttttcttc tcgtcctatc
     2641 atttggcatt ctaataaaaa cgaactatcc ttggatatag aaagagaaaa caaggaggcg
     2701 tttttcatga gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct tgcccttggc
     2761 gctgcatatc taggaaaaaa gacaggacta ttcgaagatg acagtcattt atatgatgaa
     2821 ttcgaatcaa tctagcatcc tatttagttg aatcactacg gacatacttg gaagacattg
     2881 gaggaatcac aatgagaaaa agtaaagcaa taatgattgg tgccggtatt gccaatatgg
     2941 cagcggctgt ttatttgatt caagaaggca aatggaaagg ggatcagatc accttttatt
     3001 cattggatga tcacggttca aatgatggtg caccgaccga cactgtagtg gatgaatatt
     3061 ggaataaaaa ccatccatta gaaaaccaaa aaggctatat tgctcgaggc ggtcgcatgc
     3121 ttaattaccg tacctatgta gatttgatgg atctgttaag tcgaatacct tctgttactg
     3181 agccagggat gactgctgaa gaagatacga gagattttga tgcaaaacac cgaacctttg
     3241 acaaagcacg tttgatggaa ggtggcaaag gaatcattga tggcggaaaa ctgggattca
     3301 ataataaaga ccgtattctt ctgactaaac tgattgctat gcctgattca gaagaagaaa
     3361 aactagacaa tatcactatc gaagaatatt tcaaagatga tccacacttt tttgaaacaa
     3421 acttctggtt tatgtgggaa acaacatttg ctttcagaac acgtagttct gctcaagaat
     3481 tgcgtcgcta tatgcatcaa atgatttatg aattcactca aatcgaacat ctagtgggcg
     3541 tcaatcgaac acgttacaat caatacgaaa gtattatgct tcctttgatc gagtacttga
     3601 aagaacaagg atgcaaaatc attttgaacc gccgtgtcac tgactgggaa ttcaaagaaa
     3661 caccgatgca agatgaaatc actgtgactg gtttgaaaat taccaataca ctgactcaag
     3721 aagaagaata cgttgtggtc gatgaagata cagcagttat tttcactaat gggtccatta
     3781 cagattcagc tactttagga gattacgaaa caccagctgt agaaaatatg gattatggtg
     3841 cagcttctag tctctggaaa aaagccagtg aacgtttcta caacttggga aatcctgaca
     3901 aattctttgc tgatcgagat gcaagcgaat gggtcagctt taccctgacg acgaaaaacc
     3961 atttgttgtt gaatgaaatc acccgtatca cgactcaaga accaggaaat gccttgaatt
     4021 catttatttc aaccacaccg atcacgccgt taggccaaaa agatgtcaat atgtctattg
     4081 tcgtgcatca tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt atttggggtt
     4141 atttccttta tccaagacga cgtggcgaat ttgtggataa acagtatatt aaaatgaatg
     4201 gaaaagaaat gctagaagaa ctgattggac aactttcaaa agtagatcct ggaccagtga
     4261 atattcgtga aaaagaaaca gaaattttcg acagcgtaat aaataatatc cctgtctata
     4321 tgccttatgc ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca aaagtcattc
     4381 ctaaacattc gaccaatctg gcctttacag gagaatttgt cgagcagcct tatcagatga
     4441 tctttactga acaaagtgct gtacgttctg gagaaatcgc tgctttccat tttgcgggta
     4501 ttcctatgtc ccgtcttgtc aaaacaccgc gttacgacaa agatattcct actctagctc
     4561 gtgccgcaaa aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc ctgcatcaag
     4621 taataagaac aaccaggcta aaacagcttc ttatgttttt gatttggttg gacttattac
     4681 cgcagatgca agcttttgaa caccgtttac caagaggtta tgaaaaagct gccctgcatc
     4741 aagtaataag agcagccaag ctaaaacagc ttcttatgtt tttgacttgg ttagacttat
     4801 taccgcagat gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa ggcgtttagt
     4861 ttcgagtatg aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat aaatttgact
     4921 taataccgag aaactgatcc cttctgtcat aacctctttt tatttatgtc tgtttctttt
     4981 tgaaaacaac tattttttca acgtttactc tcttgccaat ccaacatcat ctaacggata
     5041 caactatttc aatgattacc attacactaa aaaatcggaa acacacctct ccgaacacaa
     5101 atacataaga ctatagaatg tttcatttaa gaaaaaaagc gtttcattat caaaaaggta
     5161 agatctcctt taaaatgaaa ttatctgtag tactaataat gcagacaatt ttatgaatct
     5221 gaaaggagca tctccaatga aagcagtcac ttggcaagga aaacagaaaa tggaaatcag
     5281 aaacgtagat gatccaaaaa tattagaacc aacagatgca atcatccgta tcacagcaac
     5341 agccatttgc ggctccgatc tgcatcttta ccatcatggt gaagcagttt tggaaaaagg
     5401 gtatgttgta ggacacgaac caatgggaat cgttgaagaa gtgggatctc aagttcaaac
     5461 attgaaaaaa gggcaacgtg tggttatccc ttttaatatt agttgtggtc attgtcattt
     5521 ctgcctgaat cacatggaaa gccaatgtga tgaatcaaat ccagatcagt tatatggcgg
     5581 gcttttcgga tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg aatatctaag
     5641 agttccttat gcagactcta cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa
     5701 agttttattt ctatcagata ttttgcccac agcttattgg agcgttgaaa acgcaggagt
     5761 aaaaaaaggc gatacagttg tgattctagg ttctggtcca gtcggtctat tcgctcaaaa
     5821 attcgctgtt atggctggag cagagcgcgt aatcgcagta gatccagtac agcaccgtct
     5881 agataaagcg gctaattaca atggggttga aacttatttc ttagaagaca cggcacaagc
     5941 tggaacagaa ttgtatgaac tggctaaagg tggcgcagat gtcattatcg attgtgtagg
     6001 aatggacgga ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct tacaaagcgg
     6061 cacaatctct ccattacaga tggcatcgca agctgtcaaa aaatttggaa cagtccaagt
     6121 aactggtgtt tatatgactc cagcttcttc ttacccgctg caagaatttt ttatgcggga
     6181 tatcgctgtc aagcatggac aggcaccaat tatccatcta atgccaaaaa tttacgcaat
     6241 gattgcagaa ggactattcg atcctacaca aatcattact cattcgctgc cactatcaga
     6301 agcagcacaa gcatatgaga tctttgacaa aaaagaagat aaaaatataa aagttgtttt
     6361 gaaaccttga attcagtaaa aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa
     6421 tacccatgag atcgaaatag gaaaagaaga agatatcgaa atacgaggac gtcgcttccg
     6481 cttctatttc caaaatcgtt acacattgat ctcactggct gtcgatttat taaccgggat
     6541 tttctatatt tttggaagcc ttgcttcctt aactaaaatc cccgatcgat atggcaccta
     6601 tttttatttg gctggtgcta ttttcttgac gattcgtcct attctcagaa tcgtaaggaa
     6661 catttttatt tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg
     6721 agaatttttc aaatctcatg cctctttttt caccctatcg tatcactttg atacagacga
     6781 gcggtcgtcc cactttcttc cttgaccatt gtcaaaaatg tccactcgta tcgttcataa
     6841 aacgtggtat gatctgtaat taagtaaagt ttttcgtacc caagttgatg catttctttt
     6901 ctgatttccg ctaatatccg cttggctatc ccctgcttac gcttttccgg ttcaacaaac
     6961 agggcacaaa gatttggtgt cagatcaggg cggttatgga agtcgttttc aatgacgccc
     7021 gctcctccga cgatggtttc tttttcatct aagaccacat accattgagg aatactttcc
     7081 ttattagcga ttccagtacg aatactttcc tcatacacaa cagcaggaac tccccatttt
     7141 gatgaaaacc aattcgcgct ttgttttact aattctgggt gttctcttaa tttgatccag
     7201 ttatatgtaa ccaatgttta aacctccgac taaaataaaa cgtttacaaa aattatacaa
     7261 gtctttatga aaattagcaa tgacaacaat agtgatgtaa aaaaaaaaac ggaaaacaat
     7321 cacttgttct ccgttttttg attaatcaag catctaaagc ttcttttctt ctttcttgga
     7381 tcgtagctac cttgacgaac ggtagataga tcaaaataga aagaaccaaa ttgacagcgg
     7441 ctagtacacc acctgcaata ctttttgttg ccaatacacc gccaataatt ggtggagtta
     7501 cccatggagg cataacggta gctgctggca ctaaacctgt agaagtcgca aagtaagcaa
     7561 cactaactaa gaccattggt gtcaaaataa atggaatgaa catgatcggg ttcaacacga
     7621 taggtaaccc aaacatcgtt ggttcattaa tattgaagac acctggcgca atcgacaagt
     7681 ttgttacaac catatatggt ttgtttttac gtcctactaa ataaatagct aacagtaaac
     7741 ctaaagttgc acctgttcct cctaagttga caaagctgtc aaagaaaggt ttgttcacga
     7801 taaacggtgc gctatgtcca gctgcgattg cattaacgtt tgcttcaatg gctggcgcat
     7861 tgattgtttg catcaaagga tcgaccatat tcgctccgtg taaaccaaag aaccataaga
     7921 atggtgtgat aaatgcaagc agtaaggcag atggataaga gtttgcaagt cccataaatg
     7981 gttcttggac cactgtatag aaagaaccta cgatatcatc aacaccgaat gcagcaaaaa
     8041 ttgcagcaac taaagcaaac gcagagatag tgatcattgc tggtagtaat gaagcaaatg
     8101 cttttgcaac tgccggtggc acaccatcag gcattttgat gatcaatttt tcatttccgc
     8161 ttaaacgtac aaagatttct gttgaaataa tcccaacaat aatagcaata aacaaaccgt
     8221 ttgagctcat acctaatgct ccgcctaaag cgaagaaaga gcctaaagaa atcacacctg
     8281 cggaaacacc gtcttttcgg tagccgttag ctaaatgata agcaagcaag aaagcaatca
     8341 agacagaaag aatggcaaat gtaccgttcc aagcagcagc cccgaatcct ttccatgatt
     8401 caccaccaaa aattgagttc atcaattctt ggaacccggg aatcggtaag ttattgatca
     8461 ttactgctaa tgctcctaag atcattaacg gcatactcac cataaatgag tcccgaatcg
     8521 caactaaatg ccgttgtgca ccaatttttg atgctacagg tataaaatgt ttctccataa
     8581 atgcaacaaa tttatccata aatcttccct cttttcttta ctgtagatta cgcgttcaat
     8641 cgacgataca gattaatgat ctcttttgct aaatctgtaa aagctataga tgtcatcaga
     8701 tgatcttgac tgtggaccgt caaaagcgtg acttcaacgt gatttccttg tgcttcttgt
     8761 gtcagcatct ctgtttgaga gtgatgtgct tggactaaag attcctctgc atctttgatt
     8821 ttttcatctg ctaaagtgaa atcacctttt ttcgcagcag caatcgcttc cattgcatcg
     8881 ctttttgcat ttccgccaaa catgatcaga cccatgatgg cttctagatt tttttgatct
     8941 tccatttatt gtatcctccg ataattttta ttatttgttt attagttttt ctgcttggtc
     9001 gagtactttt tcaccattca tcatgccata gtcagccata ttgattacat ctaaaggaat
     9061 gcctttaggt gctagctttt cagcaaattg attttgcata aagcgtactt gcggtcctaa
     9121 aagtaaaacg tctacatttt tagattctag ttgattatcc gcatctgaag cagatacagc
     9181 aaagatgtct gcttccattc cttttgcttc tgctgccttt tgcatttttg tgactaggag
     9241 acttgtactc attccagctg aacatacaag catgattgtt ttcttttcca ttttaaacac
     9301 gttcctttct atacttacac ttgtatataa gcaagtagcg tgccaacttt aaaagcgctt
     9361 taaaaagttg atatcatcac atttaaggtc tataccaagt tgcatacaca ctaaaaaaga
     9421 aagcgcacac taaacacaca aattaatttt tattaccttt tttcttttaa cgtgcacaaa
     9481 aaaagttgtg acacaaaact tgtcacaact tttttcaaat aaacggtatt caaaagcttg
     9541 catctgcggt aataagtcca accaagttaa aaacataaga tgttgttttt actttgctat
     9601 tcttattact tgatgcagga cagctttttc acaacctctc aataaacggt gttcaatcag
     9661 ctgaccctgg gtattagctc aaaacgctgg attcacaacc tcttttattt caaataacca
     9721 gtaaatacaa tgaacatcta caaaaaactt ctatttattt atagatttcg tcaggcaaat
     9781 ccaattctct cttatcaatc aaagtctgat accacgaagc agattttttc aatgttctgc
     9841 ttcgttcatc gttcaattcg attcgaacta atccatatcg attcttaaat gcattcattg
     9901 gtgacacaca gtcagtgaat gcccataaca tatagccttc gcaatttgcg ccttcttctg
     9961 ttgctttgat caagtaatgc aaatgctgtc cgataaattc gatacggtaa tcatcttgca
    10021 cgattccttt ctcatccata tactgttctt catattctct tcccataccg ttttctgtta
    10081 tcagccaagg gatattatga taattttctt ttagatacat ggctatatca tacatgattt
    10141 tcggatagat ttcccatccc ctggaattat tcattttctt cccaggtaaa tcaaagactt
    10201 cataatatcg ttcaggatgg aaaggtgtct cttcattcca ttgataacga ggggcgcgaa
    10261 cgcgtttagg ataatattga ttgattccaa ggaaatctac tgtatgatgc tgaatcgttt
    10321 ctaaatcctc tgttgcagga tcgaaataaa tcgattgttt ttgacacaac tccaggaact
    10381 cttctgtgta gtatcctttt accatcggat caaaaaatac tcgattataa aacaaatcat
    10441 acatataagc agctttttgg tcttctggag aattggatcg agcatagacc atttcaggat
    10501 ttaaaataca gccaatcctt ccaccataat ttccgctgcg gtaaatctct actgctttag
    10561 ccgttgctaa aactttatga taattccaca tcatccactt ttttgtgttt tgttcatgcg
    10621 gccagcgaac agcatccaaa tagcaacgcg tttggggaac gattggttcg ttgaatgtaa
    10681 accagttttt taccctatca ccgtaacgat caaaggcaat ttgagcatac ttagcataaa
    10741 aatcaactac ttttcttgaa gaccagccat catatttttc caaaagaaaa gctgggactt
    10801 cataatgttc caaacaaatc atcggttcta cgtctgcttc gatcaaagag tcgatcattt
    10861 cacttatatg ctgagcatat tcttcatcta ctatacagtt ctcataatca gtaaaaaaac
    10921 gagcccaatt gatagaagtc cgataatgtg tcagatggat ggatttcatc agatccacat
    10981 cctgctggta ttgttccata aaattagttg ctacggctgg gccataccca ttatgccaaa
    11041 caaaatgttc atttttatac cagctatcta taaacgaatc ctgtccttct ttttttcctt
    11101 tccaaccttc cgtttgccaa gcagaagatg cagcaccaag aataaaattt tttggaatag
    11161 taattttcat gatttcctcc tgttttttag atcccatcat tttccacacg atttgctact
    11221 tttacaaatg gcagatagat caaaatagaa acaataatac aaacaagttg cgtaataact
    11281 gcaccgatat ttcctgctgt tgccagataa gcattgacga tcggcggcat tggccaaggg
    11341 accattacaa ctgctttcgc cgcaaatcct aaagcggttg caaaataacc aatcgttcca
    11401 gtgataagag gcgttaagat aaacggaata gctaaaattg gattcagcat gatcggcata
    11461 ccaaaaataa ctggttcatt gatgttgaag atcccaggag caagggataa cttggtgatt
    11521 tgtttcatat cttcccgttt tccaactaac aatactgcaa tcagtaaacc gatcgtgact
    11581 ccagacccgc ccatactcat atacatatcc caaaatggca tagtaataat gttcggtact
    11641 tcttttccag cttcaaaagc ttccgtattt acagcaattg ccgctaaaag caacggttcc
    11701 cgtatcggct tcaccatttg atttccatgg ataccaatca cccaaaagac ttgcgcaata
    11761 aacatcaata gcaaaatacc cggtaaccct tgtacgacac cttctaatgg gcgttgtacg
    11821 acattgtaaa tgatatcata aaggtacatt cctgtaagtt ctttgatgat aaatcccgca
    11881 gccgcgatac ccgatacagt cagaatcgtc gggaataaag cagaaaaact agcagaaaca
    11941 ttcgttggca cggtatcagg cattctcagc tgtagtttct tctgtttcct caaccagcaa
    12001 taaagttcaa tagaaaggat ggagaccaac attcccaaaa ataacccttt cgtatctgta
    12061 tattgtttag ccaatacgtt ttctactata acagtctgat tttcattcac taattcataa
    12121 acagttggat taacagataa ataagacatc actgcgatga taccagggaa tacaccatcc
    12181 actttattca gcttggctaa ttcgatccct atcagaaaaa cagcatagat ggtcaagaaa
    12241 cttagtgtga catagctgat ggcagatgcg ataggcttta attcttctaa aaaacgcaag
    12301 aaacttatct gtgctaagcc attttccgag tcaaagacca ttgctgaaaa caatgtgcca
    12361 aaagcacccg taataattac cggaatcaat gcagcaaatg cgttttttat caccatgata
    12421 taccttaaat tattgattct cgttgccacg ttccctaaaa cattggctaa tttatccata
    12481 aaactcattc cagatcccct cattcctgct tcttctcctt gacgtcatct ctttttgtct
    12541 attagtcagc gcttacattt cgatgaacag aaactattta ctcctctttt ttaccaaata
    12601 taaaacaaaa attcatctga tgtatttatc ataaaataca tcagatgaac tgtcaattag
    12661 tttctttttc tcctattatt ttctgtaatt ctctccgatt tattgccata tggacagtca
    12721 ttgcatcaaa cgcagcaagt gcattccgcc tttcgatggc ctgaacgatc tctgtatgaa
    12781 tatcgatcgt ctcgattttc agatgctgga tatcataatt tttattaaac aaagcaattg
    12841 attcattaat gattgggata atatgcagca aggctacgtt tccgctagct tctgcaatca
    12901 tcgtatgaaa atcgatgtct aattgaacat gttcttgtgt gccttctcga aaagaagttt
    12961 caatcttttg cgttaatttt ttcatttgga cgatttgatt cttcgttgca tattccgctg
    13021 ccaaacctgc catcttaggc tccaataaat atctcagttc aaataagtct cgtatcatcc
    13081 gatcctgatc ttcaatcaaa gaaaaaccga gtgggtcatt ggcaatccct cgcttatcac
    13141 tgacaaatgt ccccgctcct tgccgaactt caaggatatt cctggaagct agagcccgca
    13201 cagcttctcg caccgtgctt cgccccacct ctaaataatc ggacaattca tattcattcg
    13261 gcagtttatc tcctacttgc aactgcattt cctttaaata tctaaggatc ttgtcagtag
    13321 ttacttctac tagggtttta ttttcttcca aaccacatac ctccttttca tctgatgtat
    13381 tttttcgtaa ttattataac acgtttgaat catctgatga ttttttgtat acaaaattga
    13441 ttaatccgtt catagacata caaacgaaga agatatttac cataaaaaag actaggataa
    13501 aattcactcc tagccttcga tcatttttat tttgaacgac aaaaaatatg atgttcaata
    13561 acagacgatc ttcattcgat tcttgatgtc atcttttgtt ttttttaata tatggataaa
    13621 aagtttgatt acatcgtttc tattctagaa tttggtcgat tctcaattct tcttctggtg
    13681 agaaatctaa attatccatc attcgaacac tttcttgtaa ctgacttaac cgacttgctc
    13741 cgaccagtac acttgtaaca gatttttgtc gcaaattcca tgcaagggcc atttgcgcta
    13801 atgattgtcc tcgtgatacg gcgattgttt gcagtgcttt gattttttcc agagttgatc
    13861 caacttgttc ttctgaaagg aatggtattt ccttacgatg tgctctagaa tcttcaggaa
    13921 taccatgtag ataacgattt gtcagcaacc cttgagcaag cggactgaac gtgattgccc
    13981 ctagcccttc ttcttctaat actggagata gatcgtcttc tagtagtgca cgagaaaaca
    14041 tattgtagcg catctgatga ataatgaacg gtgcttcctt tctttttaaa atttcagtca
    14101 ttttttttgt atcttctcca ttatagttgg atataccaat atataatgct tttccttgtc
    14161 ttacaagttg catcaaagct tctgctgttt cttccaaagg tgtatcaggg tctgggcgat
    14221 gatgatagaa gatgtcaaca taatctagcc ccattctttg gagactttgg tcacaacttg
    14281 aaatgatgga tttacgagag ccccattccc catatggccc ttcccacata tgataacctg
    14341 ctttgctaga aataatcagt tcatcacggt aggcttgaaa atcttctcta aaaatacgcc
    14401 caaagttttt ctctgcagct ccagcagggc ctccataatt attcgcaagg tcgaaatggg
    14461 taattcccat gtcgaatgcg cctcttaaaa tctctctttg attatggata ggatcataat
    14521 caccaaaatt ttgccataat ccgagagata aaatcggtaa tttcaaacct gaatgaccgg
    14581 atttacgata ttccattttt tcataacgtg cactatctgc aatatacaat ttttctcctc
    14641 ctcatcaaat ctttttaaaa cgctttcttt tttctatttt tagtttacta cattttaaag
    14701 aaaaggagtt aatcaaaatg attttttcgt atattttttt gccatttgtt ttttactcgt
    14761 tgatagatca gaaattgatt acgtgcttgc tggatttttt tataacgttt cggttgtttt
    14821 ttgtaaaatt gctgatgata gttttctgct ggccaaaacg taccggctgg ttcgattttt
    14881 gtgacgattg gttggtggaa agttcctgaa ctgtctagtt tctgttttga ttgttccgct
    14941 aattcttttt gtcgttcgac ttgataaaaa atcactggac gatattgctt ccctctatct
    15001 tgaaattgtc caaatgtatc tgtgggatca gtaatttgcc aatagattgt cagcaactcg
    15061 ctataactta tttctcttgt gtcataaatg atctccacag cttccacatg acctgtgtaa
    15121 ccaccactta cttgatcata atttggtttt tcaacatgtc cgcccgtgta tcctgacaaa
    15181 acagatagga tccctttttt cgattcaaac ggctcgacca tacaccagaa acatccccct
    15241 gcaaatactg cacgttcttg atagtcagca tcttgcatgg catgcttgga aaagtcatat
    15301 ttttgctcct tatcaaatcc ttccgtaatc aataaataaa agtccattac atcaggcgtc
    15361 agattcattc ggatagctaa tggacgtagt gttgcttcta gttttgataa ctgttcttta
    15421 acggatatat tttctcttat gtcattcttt gtctgaatca atacttttcg ttcccaatct
    15481 cgtgtattcg gattcaaaat cagattataa agtgtttgaa tgaatatttg ttcctcatcc
    15541 atagattctc ctcctttccg aagaagacaa gctagtgact ttagctgtaa tttatcataa
    15601 agtgaggaaa taagcgatta cttcatcccc ctacagattt ttcgtataaa aaagagagaa
    15661 tccttatcga cagattctcc tttttaatca atttatttta taaatgaaaa gttaatttac
    15721 cttttatgga aaactatttt aaaaaataaa cataaatatg cagttaatgt aacaagataa
    15781 gaaataatag tcgttactgt ataacttgtg cttactttaa tatcagcaac tcctttgact
    15841 ccgcttattt gtaccaatcc attagcattc acctgctcct ttacaggttg atgatttatt
    15901 tctacattcg ttcctaaata agcatataca ggaagtgtga ttacttggct cttctcatta
    15961 gttactctat atatgataga actaccagtt gttttataag gaaggtttac ccattgactg
    16021 ccaatctttg cttcgtgttt tattatatgc tccttattct ccaatgaagc gataggagaa
    16081 taatcatata aaaaataa
//
LOCUS       DACDSY010000064        15642 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDSY010000064 DACDSY010000000
VERSION     DACDSY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.064"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(27..725)
                     /locus_tag="IWK44_001626"
     CDS             complement(27..725)
                     /locus_tag="IWK44_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP9550155.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="IWK44_001627"
     CDS             complement(718..2070)
                     /locus_tag="IWK44_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP9550156.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="IWK44_001628"
     CDS             complement(2293..3693)
                     /locus_tag="IWK44_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP9550157.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="IWK44_001629"
     CDS             3854..4591
                     /locus_tag="IWK44_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP9550158.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="IWK44_001630"
     CDS             4716..5195
                     /locus_tag="IWK44_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP9550159.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="IWK44_001631"
     CDS             5192..5959
                     /locus_tag="IWK44_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550160.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="IWK44_001632"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="IWK44_001632"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9550161.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="IWK44_001633"
     CDS             complement(6862..7464)
                     /locus_tag="IWK44_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9550162.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="IWK44_001634"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="IWK44_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="IWK44_001635"
     CDS             8145..8729
                     /locus_tag="IWK44_001635"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9550163.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="IWK44_001636"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="IWK44_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP9550164.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="IWK44_001637"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="IWK44_001637"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP9550165.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="IWK44_001638"
     CDS             10630..10809
                     /locus_tag="IWK44_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550166.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="IWK44_001639"
     CDS             10831..11856
                     /locus_tag="IWK44_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP9550167.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="IWK44_001640"
     CDS             12031..13275
                     /locus_tag="IWK44_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP9550168.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="IWK44_001641"
     CDS             13428..14375
                     /locus_tag="IWK44_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9550169.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="IWK44_001642"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="IWK44_001642"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP9550170.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="IWK44_001643"
     CDS             complement(15277..15519)
                     /locus_tag="IWK44_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550171.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
BASE COUNT         5318 a         2804 c         3170 g         4350 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg cc
//
LOCUS       DACDSY010000065        15615 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDSY010000065 DACDSY010000000
VERSION     DACDSY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.065"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            138..1475
                     /locus_tag="IWK44_001644"
     CDS             138..1475
                     /locus_tag="IWK44_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP9550172.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="IWK44_001645"
     CDS             1523..3652
                     /locus_tag="IWK44_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP9550173.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="IWK44_001646"
     CDS             3627..4316
                     /locus_tag="IWK44_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550174.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="IWK44_001647"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="IWK44_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="IWK44_001648"
     CDS             complement(5546..5740)
                     /locus_tag="IWK44_001648"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550175.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="IWK44_001649"
     CDS             complement(5790..6230)
                     /locus_tag="IWK44_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP9550176.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="IWK44_001650"
     CDS             complement(6234..7079)
                     /locus_tag="IWK44_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP9550177.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="IWK44_001651"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="IWK44_001651"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP9550178.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="IWK44_001652"
     CDS             complement(8703..9650)
                     /locus_tag="IWK44_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9550179.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="IWK44_001653"
     CDS             complement(9873..10223)
                     /locus_tag="IWK44_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9550180.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="IWK44_001654"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="IWK44_001654"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP9550181.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="IWK44_001655"
     CDS             11646..11966
                     /locus_tag="IWK44_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9550182.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="IWK44_001656"
     CDS             11988..12452
                     /locus_tag="IWK44_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9550183.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="IWK44_001657"
     CDS             12657..13262
                     /locus_tag="IWK44_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP9550184.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="IWK44_001658"
     CDS             complement(13301..14590)
                     /locus_tag="IWK44_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9550185.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="IWK44_001659"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="IWK44_001659"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP9550186.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT         4881 a         2908 c         3200 g         4626 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaaga
//
LOCUS       DACDSY010000066        15459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDSY010000066 DACDSY010000000
VERSION     DACDSY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.066"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            184..1029
                     /locus_tag="IWK44_001660"
     CDS             184..1029
                     /locus_tag="IWK44_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9550187.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            1220..2407
                     /locus_tag="IWK44_001661"
     CDS             1220..2407
                     /locus_tag="IWK44_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9550188.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            2413..2769
                     /locus_tag="IWK44_001662"
     CDS             2413..2769
                     /locus_tag="IWK44_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP9550189.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            2771..3109
                     /locus_tag="IWK44_001663"
     CDS             2771..3109
                     /locus_tag="IWK44_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP9550190.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            3504..4538
                     /locus_tag="IWK44_001664"
     CDS             3504..4538
                     /locus_tag="IWK44_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9550191.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            4531..5217
                     /locus_tag="IWK44_001665"
     CDS             4531..5217
                     /locus_tag="IWK44_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9550192.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            5241..6089
                     /locus_tag="IWK44_001666"
     CDS             5241..6089
                     /locus_tag="IWK44_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9550193.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            6286..7059
                     /gene="sufC"
                     /locus_tag="IWK44_001667"
     CDS             6286..7059
                     /gene="sufC"
                     /locus_tag="IWK44_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP9550194.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            7072..8358
                     /gene="sufD"
                     /locus_tag="IWK44_001668"
     CDS             7072..8358
                     /gene="sufD"
                     /locus_tag="IWK44_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP9550195.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            8355..9590
                     /locus_tag="IWK44_001669"
     CDS             8355..9590
                     /locus_tag="IWK44_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9550196.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            9577..10047
                     /locus_tag="IWK44_001670"
     CDS             9577..10047
                     /locus_tag="IWK44_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP9550197.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            10052..11443
                     /gene="sufB"
                     /locus_tag="IWK44_001671"
     CDS             10052..11443
                     /gene="sufB"
                     /locus_tag="IWK44_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP9550198.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(11538..12680)
                     /locus_tag="IWK44_001672"
     CDS             complement(11538..12680)
                     /locus_tag="IWK44_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9550199.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12719..13453)
                     /locus_tag="IWK44_001673"
     CDS             complement(12719..13453)
                     /locus_tag="IWK44_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9550200.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            13685..13960
                     /locus_tag="IWK44_001674"
     CDS             13685..13960
                     /locus_tag="IWK44_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9550201.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            14058..>15459
                     /locus_tag="IWK44_001675"
     CDS             14058..>15459
                     /locus_tag="IWK44_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550202.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHK"
BASE COUNT         5025 a         2756 c         3263 g         4415 t
ORIGIN      
        1 atacattgta aaatgaatag gaatgtatga ttacgagcaa gcagacagct gattgtcttc
       61 atctaagtta ctagttggat cagcaactgt tttgttgtac atttctatta tagatggttt
      121 tttgtatatt taaatcaaat agtttttata gtaattatca taaaaagtta tggaagtggt
      181 gccatgttta agttattaac gacttttcga gcagtatatg aaacaagaaa tttttccaga
      241 gcagcagaag tactgttttt atctcaacca gcagtctcga atcaaatcaa acagttggag
      301 cgggaattga atatcgatct ttttcaacgt aacggaagaa aagagatgct tccaactaag
      361 caagccgact tattatatga acggacactg gttttgttag aagaatggga agaaactcag
      421 caaaaattgc tcaatgaaca aaatgaagaa gaagtatgtc ggatcgttgc ttcccacacc
      481 tttgctgtct atcttttgcc tcagttgatg aaacatctga tcaaaaggtt tcctaaagtg
      541 cattttgaga tcgaaatcac gaattctcat gaatcattag aacaagtagc gaaacatgaa
      601 tccgattttg gttttatcga aaagccatta gagacttccg gcgtacaaag atactctttg
      661 atggaagacc agctagtcat cgctggggac ccaaaaagca aattgtggct tgtaagagaa
      721 gcaaactctg gtgtttttca ttacacgcag cgttattttg aagagatgaa tatccaagat
      781 aagaagatga tcgtgaaaag taacgatgta attgttgcgt tgctgaagga aggaatcggt
      841 cgctcgattt tgtctaagcg ctctgttcca gaaaatgtgc caatgactcc gttgaatttc
      901 catcgatatt tttatttcat ccaacgaacc aatctggttt ctgatcagct gctagagatt
      961 gccgaagaga tccttcgata caatgaaagc acagaaactt ctcacagcga ggagaatctc
     1021 agctggtaga taattgcatg tatcaaaaaa agttctatga tacactaaag ataagcagta
     1081 ggcttttgta ctgcatttgc tcaagcaact gcacaaacgg aaagttaatt tttcccaaat
     1141 gtctatggaa agactttcca ttatctaact ggaccgctat aataatcagc agtatggttt
     1201 ttaagaaaag ggatgaaaca tggaaaaaaa attaaaagct tcgaacgata atcgtattga
     1261 ttatggcgtg attttaccag tctttctgtt atgtttgatt ggtctgctct cattatatgt
     1321 agcacttagc catgatccga acaatccagc agtcttgaaa ggcgtaggga tgcaagttgt
     1381 ctggtattta gttggagcgg cagcaatcgt tgtcatcatg catttgaatt ccaaatggat
     1441 ctggaaactg actccttacc tttatggttt ggggttagtg gtaatgggac tcttgctcaa
     1501 attctatgat cagaatttag ctgcttcaac agggtctaaa aactggtttc gattcggtgc
     1561 gtttactttc cagccagcag agttgatgaa gattgcgtac atcttgatga tggcactgat
     1621 cgtaactaaa cataatacgc aagtaaaaga acggacgatg aaatcggact tttggctgat
     1681 tgggaagctc ttgatcgtga caataccggt tttagcattg atcatggccc aagatgactt
     1741 tggaacgatg cttgtctttc ttgcgatctt tggcgggatt ttcctgatgt ctggaatctc
     1801 ttggcgcatc atcgcacctg tagtaatctt ggcagtagtc gttggagcag gaacgatctt
     1861 cttggttacg actgaaggcg gaagagactt gctttataaa gttggattca aatcttatca
     1921 gtttgcacgt attgattcat ggctggaccc tttccatgat acgtcaggaa tgagttatca
     1981 gccggcccaa ggacttttgg caattggtac cggtggcttg tttggtaaag gattcaacgt
     2041 aagcaatatc tatgtgcctg ttcgtgagtc cgatatgatt tttaccgtta tcggtgaaaa
     2101 cttcggcttt atcggtggtg cattcgtcat tttcttgtat tttattttaa tctatcgtat
     2161 gattcgggtg tgtttcgata cgaataatga attttacgcc tatatcgcaa gcggtttgat
     2221 catgatgctg ctgttccatg tctttgagaa tattggagca aatatcggat tacttccttt
     2281 aacagggatc ccattgcctt tcattagtca gggaggatcc tcgattttag ggaacatgat
     2341 cggtatcggg ttgatattat cgatgagata ccaaaatgaa gcacctgcaa gatcttcaag
     2401 gaggtaagaa aattgtacac cttttactgg tacccgaaat gttcaacatg caaaaaagcc
     2461 aaagcgtggt tagaccagca tggcgttagt tatcaaaccg ttgatatgat caaagaagta
     2521 ccgaaggcct caacgattga gacttggcta gaaacgagtc aagtaccaat ccgtcgcctg
     2581 ttcaacacaa gcgggatgaa atatcgggaa cttggactga aagatcaagt aggcgagttt
     2641 acggcttcaa aagcgagcca agtcttatcg acagatggta tgctgatcaa gcgaccactg
     2701 atcttacgag aggaccagct tgttgcaatt ggattcaatg aaaaaattta tgaaggagaa
     2761 ctgatataaa atggcaggca agcatttgaa gaaaaaggac aacctttgga tcttatttaa
     2821 tggaaaagaa tactgtgttg gtttgacaaa tgaagcgcaa gaagaacttg gagacatcac
     2881 gtttgccaat ctgccaaaag caggacaatc gctaaaaaaa gatgaacctc tgatcgaagt
     2941 tgaagcggaa aaagcggtca atgaatttgc cagcccgcta acgggtacag tttcttctgt
     3001 taatgataaa atcggtgaag atatcaatat cttgaatgat tcagatgaaa tgaatgcgtg
     3061 gatcttaagt ttcaaagatg ttgatccaaa agaatttgac gaattataaa atcagaaaat
     3121 cacttgaaaa aagttgacac tgattcttcc agttgctata atcataagca atcctatatg
     3181 aaacgagaaa gctttgaaaa gaagagtaca tgtcacggac gtttgaagag agccctgttt
     3241 gctgaaaatg ggtaacgaaa atgagatgga agatggtctt agagcagagt aagcgagcgt
     3301 aagcaaactt actacgggag tgcccgttac agcgcgacag tatacggaaa gaagctaagt
     3361 ccaagtactg gaagaggctg ttattgcgaa gtaacggtaa atcaaggtgg taacacggaa
     3421 gttcaagctt tcgtcctttt gcttaaggca agagggcgaa ggcttttttg tttgaaaaat
     3481 cataagagaa agatgtgaat ggcatgtcat taatccaact aacgaatgtc aaaaagacat
     3541 tcaatgggaa aaacggaaac atccaagccg tcaatgacgt ttcactaaac gttgaaaaag
     3601 gggatattta tggaatcgtc ggctattctg gcgcaggaaa aagtacatta gtccgtctcc
     3661 tgaatggact tgagcttccc acgagcggcg aagtgatcgt aaacgatcaa gatattacaa
     3721 aattaaaaaa caaagaatta cgtacattcc gaaagaaaat cggtatgatc ttccagcatt
     3781 tcaacctgtt atggtcaaga actgtacttg aaaatatcca gctgccgctg gaattagctg
     3841 gcgtaccaaa gagcaaacgt aaagaacggg cagaagaact gttgcgttta gtcgggttag
     3901 aaggacgcgg acaagcatat ccttcacaac tttccggcgg acagaaacaa cgcgtcggta
     3961 tcgcacgagc attggcaaat gatcctgaga tcctgctttg tgatgaggct actagcgcat
     4021 tggacccgca aacaacagaa gaagtattag acttattgtt agcgatcaat aagaaattga
     4081 atttgaccat cgtcttgatc acacatgaaa tgaatgtgat ccgcaaaatc tgcaacaaag
     4141 tcgctgtgat ggagttgggg aaagtcgtag aagaaggaga cgtattgact gtcttcagac
     4201 aaccgaaaaa agaagtaacc aaacgctttg tacaacaaga catcgaacca gaagctgatt
     4261 cgacagaact attagctgaa ctcatcaaag aaaatccaag cggccgtttg gcaactttga
     4321 cctttagcga aagaaatgcc aacgagccag tcatttccca agcgattcga cgctataaca
     4381 tcgatgtcaa cgttgtgtat ggaaagatca aacaagtgaa agaaggttcc tttggttcat
     4441 tgactgtctt gatgactggt gaaaccaaag aattagatca agcagaagtg ttcatcaaag
     4501 aacaaggagt aggaattgag gtgatccatc gtggataaaa gcttttcaga aacatatcta
     4561 gattttaaac aaatcaaccc ggaaacgatg aatcaggcta tccttgatac actatatatg
     4621 acattaatct caatggtctt agtgacgatc atcggattag ttttgggatt gatcctttac
     4681 tctattggac gcaaaaacaa accgtatgct cgaatcattt atggaatcgt atcgatcatc
     4741 agtaatattt tccggtcaac gccatttatg atcctgatgg ttttgatcat tccatttact
     4801 aaagcaatcg taggcactat gctaggtgcg caagcagcca ttccagctct tgttttatcc
     4861 gcagcaccat tttatgcccg tctagtagag attgcttttc gtgaagtcag ccctggtgta
     4921 ctggaagcag cagatgcaat gggagcaagt tactgggaaa tcatctggaa ggtcttgata
     4981 cctgaaagta ttcccgcatt gatttctgga ttaacagtta caacgatctc gatgatcgga
     5041 tttactgcga tggccggcgc gatcggcgca ggtggacttg gaggtcttgc ttggcaggaa
     5101 ggctaccagc gtggtaatct aacagtgaca ttcgttgcga ctttaattat tttactaatc
     5161 gtatttatcg ttcaaggaat cggtgatttc ttaactaaga aaacagacaa aagataatag
     5221 gatatagggg gatgtaggat atgaaaaaga aaatttttgg atttgcagca gcgcttttgc
     5281 tgactgtagg attagcagca tgcggtaaca acagcgacag caaagattca gcaaataaag
     5341 cagacgacac gacattgaaa gtcggcgctt caccgacacc gcacgcagag attttggaac
     5401 atgtgaaacc attattgaaa gatgaaggaa tcgatcttga aatcgtcaaa ttcgatgact
     5461 atgtattacc gaaccaatct ttggaagaag gcgacattga tgccaattac ttccagcata
     5521 ttccttattt gaacaaagaa atcaaagaaa aaggatatga ctttgtcaat gccggtgctg
     5581 tccacatcga accaatgggc ttgtactcta aaaaagtcaa agatgtttcc gaattaaaag
     5641 atggtgcaac aatcattact tcaaattctg aatcagactg gggacgcatc atcacaattc
     5701 tacaagatgc tggcttagtc aaagtcaaag atggagtaga tctggaaaca gcaacattcg
     5761 atgatattga agaaaaccct aagaacttga agttcaacca tacgatcgac ccatcacttt
     5821 tagcttctac gtaccaaaat gatgaaggtg acctagttgc aatcaacgca aacttcgctt
     5881 acggcgcagg gttgaatcca ttagaagatg ccgtattgct tgaaaaggac aactctcctt
     5941 acgtgaatat tattgcgact cgtaaaggtg atgaagacag tgacaaaatc aaaaaactga
     6001 ttgaagtgct tcaccgcaaa gaagtccaag aatggatcac agacaaatgg ggcggctctg
     6061 ttaaaccagt aaaggctgat gcaaaataag gtatggaaca aaaaagatga gaaataagtc
     6121 tgtaatggct tatttctcat cttttttata cctattctaa tttagaaatc aaaattgttt
     6181 aataataaat aaaagatatt tgattttagt aaggaaggtc attgcgcttc attctcaatt
     6241 agtctaaaat gagtgagaac gaaagagaat ttgggggtat aatgcatgtc tgtcttagaa
     6301 ataaaaaatc tacatgtgtc gatcgaggaa aaagaaatcc ttaaaggcgt aaacctaaca
     6361 atgaaaaccg gagaaatcca tgccatcatg ggacctaacg gcacaggaaa atctacctta
     6421 tctgccgcta tcatgggcaa tccaaactat gaagtaacag aaggcgaaat tttgtttgac
     6481 gggaaaaatg ttttagactt ggaagtagat gaacgtgcgc gacttggctt atttttagct
     6541 atgcaatatc caagtgagat accaggtatc acgaatgcgg aattcatgcg tgcagcgatc
     6601 aatgcgaaac gtgatgaaaa cgacaaaatt tctgttatgg catttttgaa aaagttagat
     6661 gaaaaaatgg ctttactaaa tatgccagaa gaaatggctg aacgttatct gaacgaaggt
     6721 ttttctggcg gagaaaagaa acggaatgag atcctgcaat tgttgatgtt agaacctaca
     6781 tttgcaattt tagacgaaat cgattcaggt ctggatatcg atgctttgaa agtcgttgct
     6841 aaaggagtaa atgaaatgcg cggagacaat ttcggtgcat tgatcatcac ccactatcaa
     6901 cgattattga actatatcac acctgatgtc gttcacatca tgatggaagg ccgagtagtc
     6961 atgacaggga atgctgacct agctaaacgt ctggaagcag aaggctacgc tggtatcagt
     7021 aaagaattag gaatcgacta taaagaagaa gaagcgtaag ggggagcaag aatgaaagaa
     7081 aaaaactggc tcgattatct tgacgcggta aatgattttt ctttaagtaa aggcgaacct
     7141 gattggatga gaaccttccg tcaagacgca ctagcgaaag cagatgaact gccattgccg
     7201 catatcgatc gggtgaaatt ccatcgctgg tcgttatttg atgtgaaaga aacacaaacc
     7261 atcagtgaaa caggcacgat tccggcattt gatgcaatga aagacaatcc tgtgcttgtc
     7321 caacaaggtt catggacgat ttttgaacaa ctgcctgtgg aattagcaga aaaaggagtc
     7381 atttttaccg acttgttcac tgcaatgatc gaatatccag aactagtcca agaatattat
     7441 atgaaaaaag cagtaaacat gaacgaagac caattgactg cactccatgt agcttttatg
     7501 aacagcggta ttttcctgta tgtgcctaaa aatgtcgtga tcgatgaacc actcgagtcc
     7561 ttattcattc aagatggcgc atccgatgag cacttcttca agcatgtgtt gatcgtggca
     7621 gatgaacata gtgaattttc ttatctggaa cgtttccaga caaccaaaga acaagtagca
     7681 aaatcatcag gtaatatcat cgtggaagtc atcgctaaag ctggttcaaa aatcaaatat
     7741 tcagccgtag atcaattagg agaaaatatt acttcttata tgaatcgccg tgggcacatt
     7801 ttacgagatg cttctgtcga ctgggcaatt ggtgtcatga atgacggtca tgtgatcgct
     7861 gactttgact cagatttagc aggagaaggt gcacacgcag aagtcaaaat cgtggcaatc
     7921 agctcgggca gacaaatcca aggaatcgat acacgagtaa ccaataaagc ccctcatacg
     7981 atcgggcata ttttgcaaca cggagtgatc cgagaaaaag gaacattgac ctttaatggt
     8041 atcggccaca tcttgaaagg ggcaaaaggt gcagatgccc aacaagaaag tcgtgtcttg
     8101 atgctttctg ataaagcacg tggtgatgcg aatcctattc ttttgatcga tgaaaacgaa
     8161 gtaactgctg gacatgctgc cagtgtagga cgagttgatc cagaagaaat gtactatctg
     8221 atgagtagag gattgcataa agaagaagca gagcgcttag tgattcgagg attcctcggt
     8281 tctgtcttaa cagcaatccc tgtagaacaa gttcggaaag aattagtcga agtaatcgaa
     8341 gggaagttga atggatgatc gatgctaaaa agctacgcaa tgattttcct atcttatttc
     8401 aagacgtcaa tgatgaacct ttagtttatc tggataatgc agcgacgacc caaaagccaa
     8461 aacaagtact ccaggcatta gcgtactatt acgaacacga taacgccaat gttcatcgcg
     8521 gtgtccatac gttagcagaa cgcgcgaccc atgattatga acaagcaaga gaaaaagtac
     8581 gggcgttcat ccatgcaaaa gaaacagcag aagtgctgtt tactcgtggt acgacgacaa
     8641 gcttgaattg ggtagccaga accttcggcg accgctatat caaagcagga gacgagatag
     8701 tgctctctta catggaacat cattctaatg ttatcccgtg gcagcagttg gcaaaacgaa
     8761 caggtgctgt cttgaaatat atcgaactaa cagaagaagg cttcctggat atggaggatg
     8821 cagcgaaaaa gattacggac aaaaccaaaa tcgtctccat tgctcatgta tccaatgtct
     8881 taggtgtctt caatcctgtt caagaattag ctgaacttgt ccatgaaaac ggcggggtca
     8941 tcgtagtaga tggtgctcaa tctgccccgc acatggccat tgatgtccaa gcactagact
     9001 gtgatttctt tgctttcagc ggacacaaaa tgtgcggacc aacaggtatt ggtgttttat
     9061 atggtaaacg aaaatggtta gaagaaatgg aacctgttga atttggtggc gaaatgatcg
     9121 attttgtcga attatacgac agtacatgga aagaactacc ttggaaattc gaagcaggga
     9181 caccaaatat cgccggcgct atcgcattag gccatgcaat cgattatttg caaaaactcg
     9241 gtatggacaa tatccatcga tatgaagaag aactagccgc ttatgtgttg ccaaaattac
     9301 aagcaattga tggattgact gtttatggtc cacaagaccc gaattttcgg acaggagtcg
     9361 ttgcattcaa tttggatggg ttacatcccc atgatgtcgc gacagcttta gacatggaag
     9421 gggtagccgt cagagcaggt catcattgtg cgcaaccgct attgaagtac cttgaagtat
     9481 cagcgactgc tcgcgcgagc ttttatttct acaacacaac gcaagacgcc gaccgtttag
     9541 ttgaagcaat catggcaaca aaggagtttt tccaacatgg gactatctaa attagacaat
     9601 ttataccgtc aagtgatctt ggatcattct tcccatccgc atcatcgtgg aagcttgact
     9661 gactcgacaa gcaagatcga attgaacaac cccacatgtg gagatgtgat cgaattgcaa
     9721 gtcgatgtaa aagacggaat catcaaaaat atcgcctttg acggcagtgg ctgctccatc
     9781 agtacggcaa gtgcctcgat gatgacagac gccgtcatcg gaaaatcaat agaagaagca
     9841 gaacatttaa ctgtagactt tctacagctt gtccaaggaa aagaagtcaa agatcccgac
     9901 caattaggcg atgctgctat gcttggtggt gtagctaaat ttcccgctcg gatcaaatgt
     9961 gcgacacttg cctggaaagc actaggaaaa gcaatggaaa acgatggtac cgcagctttc
    10021 catcattacc acaaggaaga ggagtgagaa catgggcgta ccagaattag aagaatataa
    10081 attcgggttt cacgatgatg tcgaaccggt tttcagtaca ggagaaggct tgacagaaga
    10141 agtgatccgt gagatgtcaa gaatcaaagg cgaaccagaa tggatgcttg aattccgtct
    10201 gaaatctcta gaaacattta acaaaatgcc aatgcaaaca tggggccccg acctttcaga
    10261 catcgatttc gatgccatca aatattacca aaaaccaagt gacaaacccg ctcgtgactg
    10321 ggaagacgta ccagaaaaaa tcaaagatac atttgaaaag attggtatac cagaagctga
    10381 acgagcttat ctagccggtg cttccgcaca atatgaatca gaagtcgtgt accataatat
    10441 gaaagatgaa ttcgaaaagt taggaatcgt ctttactgac actgactctg ctttgaaaga
    10501 ataccctgaa ttattcaaag agtacttttc aaaacttgta ccgccaaccg acaacaaatt
    10561 agcagcgctg aattcagccg tatggtcagg tgggaccttt atttacgtgc caaaaggcgt
    10621 caaagtcgat gttccattac aaacgtactt ccggatcaat gcggaaaaca cggggcaatt
    10681 cgaacgtacg ctgatcattg ttgacgaagg agcaagcatc cactatgtcg aaggttgtac
    10741 ggcacctact tattcaagta acagcctgca tgccgccatc gtagaaatct ttacaagaaa
    10801 agatgcgtat tgccgttaca caacgatcca aaactggtca gacaatgtgt acaacttagt
    10861 aaccaaacga gcaaaagcat acgaaggcgc aaccgttgaa tggatcgacg gaaacctagg
    10921 tgcaaaaaca acgatgaaat acccaagtgt ttacttagat ggaaaaggtg cacgaggcac
    10981 catgctctcc atcgcctttg caggagcaaa ccaaatccaa gatacgggtg caaaaatgat
    11041 ccacaatgca ccaaatacat ctagctctat cgtctctaaa tcaatttcaa aagacggcgg
    11101 agaagtaaac tatcgaggac aagtaacctt tgccaaaaac agtgcgggtt cgatctctca
    11161 tatcgaatgt gacacgatca tcatggacga atggtcgaaa tccgatacga tcccattcaa
    11221 tgagatccac aacagccaag tctctttaga acacgaagcc aaagtctcaa aaatctcaga
    11281 agaacaactc tactatctga tgagccgagg cctgaccgaa tctgaagcaa ctgaaatgat
    11341 cgtcatgggc tttgttgaac cattcaccaa agaacttcca atggaatatg cagtcgaact
    11401 caatcgattg atcagttatg agatggaagg cagtgttggg taaacctgtt ataggagcta
    11461 gaacccttga tatataaggg ttctagcttt tttgttttgc ctgtatacta gaaatgacca
    11521 gattttgacc agctctttta ctgatgagcc atcatatcag caaagtcttg acctgttttc
    11581 ttaggtgcat tatttgtaat atgtgcatag atgtctaatg tcactttact attttcatgc
    11641 cctaatcgtt gttgaactac cttgacagat tctcctgctt ctaataataa actagcatgt
    11701 gtatgtctaa acccatgagg tgtaatacga ggcaagttat attttttcaa aatatatctt
    11761 aaccaatcat taacagtgtt tggaatgtat agagtgtttt taactgttgt aaaaacatgt
    11821 tgctctttag agctagtatt gtatccaagt tttaaatact cttctttttg ctgtaatttc
    11881 cagttgttga ggatagaaag agtctcagta tctaaagaga taactcgtct agaagctctt
    11941 gttttcggag attgaattat aactttacca aattcatcta cagcaagagt tttattgaca
    12001 tgtagttctt tattaaacgt gtctatgtct ttccactgta aagcgagtac ttcactcttt
    12061 ctcatacctg tgtatgccaa taatctgaag aaggcgtatt gtttcatgtt accaaaatct
    12121 ttaaaagcat ccagtaaatt attaagctcc tcttttgagt aaaatttacg atttttctca
    12181 tactcttttt ttcgtggaag aagtgttttt tccataggat tattgtcaat atattgcatt
    12241 gcaaccccgt agcgtaaaat ttgtgcagtc tgttttctca tataatcgta actttcatat
    12301 ttactgtgcc attcatctac cactttttga caatatggga cagagatatt cgatagttta
    12361 agcttaccaa atgctggaag tatttgatta tttgcgtatc ttactgagat tgcgattgtt
    12421 gaagctttaa cactggtacg ctgatgagtt atccattcat tatacaggtc ttcaaatgtc
    12481 atatcaggtg ctttcttttt aacaccgtat aatatttgat gtctgtactc gtcataatct
    12541 ttttgagctt cttgtttgct actaaaacct cttctcgtag tatttatttt ttcaccagtt
    12601 cgtggatcgg tggcaatgtg tcctttatac atccacgcca cttgaccgtt tttctttttg
    12661 tactttgata tttttgccat taaatcatct cctcttagtt tacaaggagt acttaccact
    12721 agatggtatc acctcccttc atttttttct tagtgtattt tttcccaatt gatgaaatcg
    12781 tttgttctag cttgtttata ttatcagtaa tttctctcac agcttcttcg ggataagaca
    12841 tgtctttcaa ttgaataggt agccattcat ctaccattct ttctagtagt tcttctatac
    12901 tgctagtgag gaaaagaatg ttattgtcaa attcttctac aatagcattg atgaataatt
    12961 cttctatttt taagatattg aaaagactaa ttttttcatt tttaacttta ttacaagtct
    13021 tgttagcaat aaattcttgc atctctggtt ctaatttgga atatctatcc agccatgtat
    13081 ctaaattaga gtgtactggt gggaactgtt gacgaagatg ctggaacagg tttttatctg
    13141 gaaatgttat accatctagt acaatgctat ctctataaag aataattccc aaaatatatt
    13201 cttctacatt gccacaaact aactcctcta tactcatatt tcccaaatca gcaattcggg
    13261 ataatcgctc tggtattggt aaataggttc ccttctccca ttcgtagata gtttttttgt
    13321 caattaattg accgttgtta atcataagac caaatgctgg ttgtgttaat tttttactct
    13381 ttcttatagc ttttattttt gttccgagta tcttttttcg ttctaaataa tttattggtg
    13441 gacgttttat catattgacc acacctccta aaattagtat acctcaaaaa aggggtgttg
    13501 caaataaatt ttacaaagct gttgacaccc cttttttatc gaggtattat gtgtatgtcg
    13561 atgaactggt aatcattttt tgtttgatta atcggttaat aacaaaatac ttttattttg
    13621 agcaaaaaat acgaagataa ttcttagaaa atttgttcaa gcaatcacga aaggaggggg
    13681 aaatatgtct ctacaaatca ttgatagcat tgtttctata caagttgaac aacaagtgaa
    13741 agagataatt acagtgctaa agcctatgct agaagacatt atttctggag tcactctaga
    13801 gtatatggac tatcagcagg caagtgaata tctaggtgta tcagtaggaa cgattagaaa
    13861 atatgtttct caatacgggt tacctgttat aaaaattgat acggtcatta gatttaaaaa
    13921 atctgatatt gatagtttca tggaacggta taaaggttga gtaaacaaat aagactaaaa
    13981 catatagtaa taaagagttt gtttttatta tctgtatgtt ttagtcttta acagaatttt
    14041 ataaggagga aacacaaatg gaaacatcaa tgattaatat tccaatttat gtagatcaag
    14101 aaaaattatt agagaagcca gttacaaaaa aagaaaaaac tgatgtaaga gataggtgtt
    14161 tagataacta tagagaagtt acaagagatg agtttgtaga gatactaaat tcatcacaat
    14221 catttattcc aagcaagaca aagaataaag gcaatgacaa ggagagtttt gtggaaacca
    14281 gagtaattat tctggatgtc gataacactg ttaaagatga gaaggggaag gtagtagatt
    14341 tgagtaaaga tgatagtaga tacttaagcg tagaaaaggt tttatctatc aatttagttc
    14401 ataattctgc gtttgcgata caaaaaagta ttcggtactc tgaaaatcta gaaaaataca
    14461 agattgtctt tctattaaaa gaagcaatta ctgactatgg taaaatggtt gctgtttacg
    14521 aatacttgaa aaagaacatc cccgggtgtg atgacaatgt aaatgcttcg aatagaatgt
    14581 tttttggagg atataagtca gatgcagtga taataattaa tgaagacaac atgttagata
    14641 ttactgagct tccgattgat tttaccatga catgttctaa aaattgtcaa agtgattcag
    14701 ggagagagta tgaactagtc aatgaaaccg attttgttaa gttgataaaa aatgacgaca
    14761 aagaagaaat gaaacagtgg tttaaagact gcctgtttga ttcctccgac atgtctttta
    14821 atgagattta tgaaagattg ttaaccattg atatgaataa attacttaag tcaagtaaaa
    14881 acttaagatg tctgtttcac aacgatcata atccctctgc tagtattttt accagtaaaa
    14941 ctgggcattc cattttttat tgtcattcca gtaattgtga tatcagtgaa aattttattg
    15001 gagttgtgat gctaatttta aagaaagaca gtcgggtaga aacatttaat tggctattag
    15061 agaacctaga cttgtatcct acacattttc gaaaattgaa agaggaatca aaagtacttt
    15121 ttgacactct tgaatcaatg aaagacaaac ttttcaggac aataaggaat tatctagagg
    15181 aaatgcgacg tgtctatgat gtcttgcttt ctgatgttaa tttttttgat gatagtaagg
    15241 aaagtgtgac ttgtattttg tcgggggaac aattagctaa aaaaatgagt tcttactatg
    15301 gtaaaactta tgacatagat aaatgtaata agttactatc acttatgact tttataggat
    15361 tgataaaaaa attagatgat gaagatgttc ctttgaagat gctcgaatac ctaaataggt
    15421 taaaggaaga gaaagcgtta ggaaacttta atcataaga
//
LOCUS       DACDSY010000067        15416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDSY010000067 DACDSY010000000
VERSION     DACDSY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.067"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            211..1977
                     /locus_tag="IWK44_001676"
     CDS             211..1977
                     /locus_tag="IWK44_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP9550203.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2079..3338
                     /locus_tag="IWK44_001677"
     CDS             2079..3338
                     /locus_tag="IWK44_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9550204.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3485..4786
                     /locus_tag="IWK44_001678"
     CDS             3485..4786
                     /locus_tag="IWK44_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9550205.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4789..5640
                     /locus_tag="IWK44_001679"
     CDS             4789..5640
                     /locus_tag="IWK44_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9550206.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5653..6468
                     /locus_tag="IWK44_001680"
     CDS             5653..6468
                     /locus_tag="IWK44_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP9550207.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6619..7785
                     /locus_tag="IWK44_001681"
     CDS             6619..7785
                     /locus_tag="IWK44_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9550208.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8015..8662
                     /locus_tag="IWK44_001682"
     CDS             8015..8662
                     /locus_tag="IWK44_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP9550209.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8746..9690
                     /locus_tag="IWK44_001683"
     CDS             8746..9690
                     /locus_tag="IWK44_001683"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP9550210.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9825..11276
                     /locus_tag="IWK44_001684"
     CDS             9825..11276
                     /locus_tag="IWK44_001684"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP9550211.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11297..12133
                     /locus_tag="IWK44_001685"
     CDS             11297..12133
                     /locus_tag="IWK44_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9550212.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12133..12792
                     /locus_tag="IWK44_001686"
     CDS             12133..12792
                     /locus_tag="IWK44_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9550213.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            12918..13607
                     /locus_tag="IWK44_001687"
     CDS             12918..13607
                     /locus_tag="IWK44_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550214.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13613..14569
                     /locus_tag="IWK44_001688"
     CDS             13613..14569
                     /locus_tag="IWK44_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP9550215.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14613..15125
                     /locus_tag="IWK44_001689"
     CDS             14613..15125
                     /locus_tag="IWK44_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9550216.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
BASE COUNT         5054 a         2706 c         3150 g         4506 t
ORIGIN      
        1 aattgatcgc ttaagataaa ttcaggacac taaatagtac aggaagtaga ctaaaacttt
       61 taaaagtttt aagtctatct atttggcaag tttaaaaaat aaaaattcgg tatcaaaaaa
      121 ctgttaccga taacattttt gaaagcgttg tcttttgctt ctctatcact tatagtgatg
      181 acgtcatatt aaaacacaag gaggaaatca atggatacag ctgcaattta tcatcgaccg
      241 gaaagtgaat atgcatttct atatacgaat acaagatttc gtattcggct tcgtactagg
      301 aaaaatgata tcaaaaaagt atatgtgcta tgtggagatc catataccat aacaactgaa
      361 aaatggtatc aaaaacaacg accaatgaaa aaatgtctaa gtaccaatgt tcatgattat
      421 tgggaaattg aagtgacgag cgaaacaagt cgcttacagt atgcgttcca tgttgttggg
      481 gaagataaca cggactgttt ttacggtgat caaggaattt ttccttatcg agaaaatgtg
      541 attactgaac caaattttta ttttcgaata ccttattttc atcaaatcga tcgattcact
      601 attccagaat gggtaaaaga aacagtatgg tatcaaattt ttcccgaaag atttgcaaac
      661 ggggacaaag caaatgaccc cgaacacact ttaccttggg gaagtaaaga tccagaccga
      721 gaagactttt tcgggggaga tttgcaaggt gtgatggatc atttggatta cttgacggac
      781 cttggcatca atggcatcta tttttgtccc atatttaaag ccacatccaa tcataaatac
      841 gatacgattg attattatga ggtggatccc gcttttggcg ataaaaaact tttaaaaaac
      901 ttgattgatg aagcacataa aagagggata cgtatcatgt tcgatgctgt attcaatcat
      961 atgggtgtcc attcaccaca gtggcaagac gttctcaaaa acggagaaaa atcgatctat
     1021 aaagactggt tccacatccg ttttttccca gtagattctt atcagatgac tgacttacca
     1081 gaaactgctg aaaatatccc atatgatact tttgctttta ctccattcat gccgaaacta
     1141 aatacagcaa atccagaagt gcaaaaatat ttgctggata tcgcaactta ttggataaaa
     1201 gaattcgata ttgatggatg gcgtcttgat gttgctaatg aagtcgatca tcatttttgg
     1261 aaaaaattca gagaagcagt tacagaaatc aaaccagaca tctatatcct tggagagatt
     1321 tggcattcct ctcaagcttg gctgcaaggg gatgaattcc atgcagtgat gaattatgca
     1381 tttacagatt cgattaaaga ttattttgca aaaaagaaaa tcacagcttc acaaatggtc
     1441 agtggaatga atcatcagca aatgctttac agagatcagg tgaacgaagg aacattcaac
     1501 ttattagatt ctcatgatac tgccaggatc ttgacccttt gccaaggtaa caaagaattg
     1561 atgaagtctg tgatggcctt tatgtttctt caaaaagggt ctccttgtat ctattacggg
     1621 acagaaatag ggatgactgg tgaagatgac ccggattgcc gcaaatgtat gatatgggag
     1681 aaggaggaac aagatctaga gctattcggt tttataaaag aacttgtttc tctaaggaaa
     1741 cagttgtcta agattatttc tgaaggttct actcagtggc taatagtaaa tgatcgtgaa
     1801 gataagctct atttcacacg gaaactagag ggacagatca tctatgttta ctttaatcag
     1861 tcaaaagaac cttgggtagt agaacaggaa aatgaggtga ttcttagtca aaattgtcag
     1921 ttgttagaag acgggaaagc agagatacga caatacggat ttttgatcat gtgttgaaaa
     1981 ctgttaccgg taacaacaag aaagcgtttg caaatgaaga tttgtgccca ttataataag
     2041 ggtgtaacag cacgttgaat acaaaggagg aaatgaagat gaaaaaaaga agtgtgaaac
     2101 agttgggttt agggttaatc tctttaggaa tcttaggcgg attagcggct tgtggcaatg
     2161 gtaactcttc agcagaaggg gaagccgatg aaggaaaaac gttaacagtt tccgttgatt
     2221 ctggatataa agattatata aacgaaataa aaaacacttt cgaaaaagaa aacgatgtga
     2281 aaatcaagct agtagaaaag gacatgttcg atcagttgga atcattagcg ttagatgggc
     2341 ctgctggaaa gggacctgac gttatgatgg cagcatatga ccggatcggt gcgttaggac
     2401 agcaaggaca tctggcggaa gtaaaactgg gaaatgaagc agcttacgat gaaacagaca
     2461 aagcccaagt gacttatgat ggaaaaatct acggagaacc tgcagtaatc gagacccttg
     2521 ttttgtacta taacaaagat ttagtcgata cagcaccatc aacgtttaaa gatttggaga
     2581 atctatccaa agacagtcgt tttgcttttg agtcagaagc aggaaaaaat acaggatttt
     2641 tagctaaatg gactgacttt tattattcct atggtttaat tgcaggttac ggtggctatg
     2701 tatttggtga tgatggcaca aatccttcag atatcggttt gaataatgca ggtgctgtag
     2761 aaggaatctc ttatgcaaca gattggttca aaaatgtttg gccaaaggga atgcaagaca
     2821 taaaaagtgc aggagacttc gccagccagc aattcatgtc caataaaact gcagcaatca
     2881 ttgacggtcc atggcaagcg caaacataca aagaaaacaa tatcaattat ggtgtagcca
     2941 aaattcctac tctaaataat ggacagcctt atcaaccgtt tggcggagga aaaggatggg
     3001 tagtcagcaa ttatgctaaa aacaaagacc tctctcaaaa atggcttgat tatgtaacga
     3061 atcaggaaaa tcaagaaaaa ttctttgaaa tggtaaatga aatccctgca aaccaacaag
     3121 ctagggaaac agcaaaggct aaaaacgatg aattaacaac agctgtgatt gaacaatatg
     3181 aaacagctca agcgatgcca aacatcccag aaatgggcga agtttggaca ggtgcagaaa
     3241 acttgatgtt cgatgcagca tcaggttcaa aaacaccaaa agaatccgca gatgaagcag
     3301 taaaaacaat cagtgaagcc atagaacaaa aatactgatt aaaatgataa ccgtccattt
     3361 ttataaaaaa tggaacaggt gcttcagata aaagtggcaa gagcgagatg gaaaattatc
     3421 ttgctcttgt tgccattatc ttaaaagcag tttgcggttt agcaagttta gagggggcat
     3481 tcagatgcag caagataaaa caagcaatat caacgaaaaa aaaatcacgc tttgttcact
     3541 tattccaggg atggggcagt ttgtaaatgg ccaagctttt aaaggtatcg tttttttcgc
     3601 catattcgtc ctattcattg gtcagatgct tctgggcgga tttcatgcat tggtaggatt
     3661 ggttactttg ggaagtatac caatggaaga tcattcatta tttattttga ttgaaggcac
     3721 tttgcaattc atcattacaa tcattttctt gattttttac ggtttgaatc ttttagacgc
     3781 ttatcgagta gcgaagatgc gtcaagaggg caaaaaaatc aatcggacga tgaaggaagt
     3841 catacataat ataggtgatg aaggttttcc ttatctattg acacttcccg cttatcttct
     3901 catgattttt acgatcattt tcccagtcct agttacattg ttcactgcat ttactaatta
     3961 tgacttcaat catattccac cagccagttt aattgattgg gtaggggcta aaaacttttt
     4021 tagtatcttc tttttaagct catatcgtaa tacttttttt gcagtctttt cgtggaccgt
     4081 gatttggact ttatgtgcaa caactttgca aatcaccctt ggtattctca cagcggttgt
     4141 tgctcaccag aaatttatta aattcaaacg tattttcggt atcattttct tattaccatg
     4201 ggcggtccca gcttttatta ctatcatgag tttttcgaat atattcaacg atagcgtagg
     4261 tgccatcaat actcaagtga ttcctttcat aaaccagctt ccttttgtag atatcagtgc
     4321 aatctcttgg aaaacggacc cgaactggac gaaagtagca attatcttag tgcagggctg
     4381 gttaggattt ccctatatct atgtgatggt tacaggaatc ttacaatcga tttcagaaga
     4441 cttatatgaa gcagcaaaaa tcgacggagc aaatgcatgg cagcgctttt caaacatcac
     4501 gcttcctgct gtcttcctag tggcagcgcc aacatttatt acacagtata cggggaattt
     4561 caataatttc tccatgatct atctgtttaa caatggcggt cccggaagcg taggcggagg
     4621 agctggttct actgatattc ttatttcctg gatctataaa cttacgactg gaacttctcc
     4681 gcagtattct atggcttcag caatcacgtt gattatctcc gtcatcgtta tctgtgtgtc
     4741 attgctggta tttaaaaaga caaatgcgtt caatatgggg gattaacaat gaaaaaattt
     4801 cattcgagtg ctcgattcca aaaacgactt tctcactttt ttacttactt ttttctttta
     4861 atactgtcag tcattatcat ctatccgctt ttgatcacag caagtactgc cttcaagtca
     4921 ggaaatattg cagcttttag tttagatctt tcaagtgact ggactttagg aaactttact
     4981 cggctattca gtgaaacact ttacgggact tggtataaaa atacattgat catttctgtc
     5041 ctaacaatga tcgtacaagt gactgtagta actttggcgg gctatactta tagtcgctat
     5101 cgttttatcg gccgcaaatc aagtttgatg ttctttttag tgattcagat ggtaccgact
     5161 atggcagcat tgactgcctt ttatgtcatg gctttcttat tagaagcgct cgataagtac
     5221 tggttcttga caatgatcta tataggcggt ggaattccaa tgaatacttg gctgatgaaa
     5281 ggatactttg acactgtacc aatagattta gatgaatctg ctaaacttga tggggcaggg
     5341 catttcagga tttttttcca gattgtatta ccgcttgtca aaccgatgct cgccgttcaa
     5401 gcattatggg cttttatggg accttttggg gatttcatgt tagctaagtt cctgcttcga
     5461 acacctgaaa atcagactgt tgctgtcggc ttacaaacat ttatttctga tgtgacaaac
     5521 caaagagtct ctctttttgc tgctggggca attttgattg cactaccgat ttgtctatta
     5581 ttcttctttt tacaaaagaa ctttgttacc ggcctgacat ctggcggaac aaaaggatag
     5641 gagcagagta tcatgcaaac atttccgtta aactattttt ccgcattacg tacgccaact
     5701 caagtgtttg cgggacgcaa actattatca tggcctaaat ttttcttgat atttgtattt
     5761 ttagtttcct tgatggtcat gcctgtgact cttttttatg ccaatcagat ccaagcgatt
     5821 ccattagaac aatttctatc cgttcattcg ctgatcgatg aacaaggaac ccaaaaattt
     5881 tctgaacttg aattatctga aactgggtta caagcatcgc aacaaacgat agctgtgacc
     5941 cctgaaattt tggtaggtgt gtctttatct gagaaacaac aatccgatca tggaactttt
     6001 attgattttg agaaggaaca atgggtgatc caacaaaagg ataaatctgg tatacgccgc
     6061 tatacgatga attatagccc atctttccaa ccagattctg tacgtacgcc cgaagatttc
     6121 caacgatttt tagaaagaga attttatgcc agtaatcgtc caacaatcat tttatcttat
     6181 agtttaagtc ttggtcttgt cctatttgtc atgacgagtc tcattttatt cggggcttcc
     6241 tttttcttgt ggatgacaag aaaaagccaa ctttcatcca ttcatacatt caaagagagt
     6301 gcaaatttga tgctgaatgt tatgggaata ggttccatgg cagcagcagt tgtagggttt
     6361 atccactttg actttatctt gatgctaagt gtccaaacaa cgataactgt cttgctgctt
     6421 ttatgggttt ttgtcaaaac aagattcaaa gataagcgtg tcgaatgaca agtttaagcg
     6481 atttatgcga caatgaatgt gaaaaaaatg aaaaataaag tagtttttga aaaatttctg
     6541 aaaaaattaa acttcttagt tgattctttt cttttttagt attaacgtaa tagggtagtt
     6601 tttttaagga ggaaaatcat gttagagaaa actttataca atcaattatt ttcacgttct
     6661 ttctcgctcc ctgtcgaagt aaattattgg gacggtacaa cgaaacaata cggagatacg
     6721 gacactcctc cccaaatcaa aatcaatatc catgaagaaa tcccaatgaa agagattaca
     6781 agtaacgctt ctttggcact aggagaagcc tatatggatc atcgaatcga gatcgaggga
     6841 agtatccaag cattgatcaa cgatgcttat cagcaagcaa acagcttttt acgaggaaaa
     6901 gattatctaa aatggctgcc aaagaaagaa aaacatacga aacaaaaaaa caaagaagat
     6961 atccatgcgc attatgactt aggtaacgac ttttataaac tttggctaga tccaactttg
     7021 acttattctt gtgcttattt caatacacct gaagatacgt tggaacaagc acaaatcaat
     7081 aaagtccatc atatcttaga caaactcttt atcaaagaag gcgaaacgtt gcttgatatc
     7141 ggttgtggct ggggaacatt gatgttcact gctaccaaag agtataatgt caaggcaact
     7201 gggatcacac taagtgaaga acaatacgat ttgattacca aaagaataaa agaagaacat
     7261 ttagaagata aatgccgtgt attattaatg gattaccgcg aactaaaagg cgaaacgttt
     7321 gatcatatca caagtgtagg tatgttcgaa catgtaggtt ctgaaaatct tgaaggttat
     7381 ttcaaagtgg taaaagattt attgaaacca aaaggaacag ctttgatcca tggaatcagc
     7441 cgacaacaag gcggtgcgac aaatgcttgg atcaataaat atattttccc tggcggttat
     7501 gtccctggtg ttactgaatt agttgataat attacaaaaa acgatttgca actgattgat
     7561 ttggaaagct tacgtcgtga ctaccaattg acattagaac attggacaaa gaattttcat
     7621 caagtccaag atcaagtcac gaaagaaatg ggtgattcct tctatcgaat gtgggatcta
     7681 tatttacaag cttgtgcagc ttcatttgaa tcaagtaata tcgatgtgat tcaatatttg
     7741 cttgttcaac ctggaaacaa tgacatccca atgcatcgtg cataattgag gcaaaagtac
     7801 tgtttaaaga ctatttaaaa agaagctaag ttttatgatg ctccagaagt gaactttttt
     7861 gtctaacttc taggattgtc tctaaagctt agtttctttt tctttatatt ttcataataa
     7921 aagtttggtt ctttaatttt taaacatgac agaaaatttt ttcttcgtta tacttgtaat
     7981 agaatactat tttataacaa aggggatcga atcaatgaaa aaaactgtta tttatactag
     8041 tttatttgca gctttgtttt tagctgcttg tcaatcaaat accaatcaag gcacatctgg
     8101 gaatacgtct tccagtagcg aacagcgctc ttcacaggta tcggactcct caacaaaagc
     8161 aagcacatct tcgtctagcc agacaaaaag ttctagtgaa gcaagtactc agtcagatat
     8221 caaagtgtct gcagctcgtg cgatcgaatt gttccaagat aaatacccag atactgcaat
     8281 tactagtttg gaattagata gtgattgggg atcttacttc tacaagatag aaggtgtcga
     8341 tgatcaaaaa gaatacgaag caagaattga tgctcaaaat gaaaaaatgg agacagagaa
     8401 tccagaaact ctagatcgag atgaacaaaa tggtcaaaag aaaaaagaag atggtctgga
     8461 tgttgataaa ttgatcacga ttgaggaagc agcaaaaatc gccgtcgaca aagtaggaag
     8521 cggtacggca acagattggg atttggataa agatttaggt actacttatt gggaagtcaa
     8581 agtaaaagac ggcaatcaaa gtacatcagt caagatcaat agccaaacag gcgaagtact
     8641 gtcaactgaa caagatgatt aagaatttct tcggaaaaca agagttgatt tttcttttag
     8701 tcatgataca ctagacaaaa taacttgttt gaaggagcga tttacttgag tttagaaacg
     8761 acctttgcca atcatatctt tgccaatcct ttgatgaatg catctggtgt ccactgcatg
     8821 actacccagg aattagatga actggctcat tcagaagcag gagcatttat cactaagagc
     8881 tgtacgatca atgaacgaaa aggaaatccc gaaccacgct attttgatgt tcctttaggg
     8941 agtatcaatt ctatgggact tccaaactta ggtttttctt attatctaga gtatgctctt
     9001 gcgtatgaaa aagtgcagga aaaccaaaat caaccgttat ttttttccat tgccgggatg
     9061 agtgtccagg aaaacttgga aatgcttgag aaaatagaaa aaagtgggtt taatgggatc
     9121 actgaattaa atttatcttg tcccaatgtg cctggaaaac cacaactagc ttacgatttt
     9181 gaggccactt atgaaacact gaaagaagta ttcagcatct tttctaagcc gctaggaatc
     9241 aaattgcctc cttattttga ttttgctcat tttgatcaga tggctgatat cttgaatcag
     9301 tttccattga cctatgtcaa tgctatcaac agtgtgggca atggtttgta tatcgataca
     9361 gaacaagaag cagtagtcat caaaccaaaa gaagggttcg gcggcattgg cggagagtat
     9421 atcaagccga ctgctttggc gaatgttcgc gctttttata cacgtttgaa accagaaatc
     9481 caaatcattg gtacaggtgg gattcggaca ggacaagatg catttgaaca tctgctatgt
     9541 ggcgcaagca tgttacagat tggtaccgag cttcataaag aagggccgga aattttttct
     9601 agaatcataa aagaattaac gcagattatg tcagaaaaag gatatacgtc aattgacgaa
     9661 tttaaaggca aattacgcac catttcatag caaagagacg aaggtcatga ttgatcttcg
     9721 tcttttttta taaaaaacac tcgtctgttc attcctgtgc aagcgtactc attgtatgtt
     9781 agacgaactt gtgggaaaat aacagaggca aaaaggagga taatatgaat gatgcaacag
     9841 aattagcaaa agagcttaaa gaaaaagaat ttagttttca agaactagtt acggatatac
     9901 agaaaaaaat aaagaagcaa aatcatgaat tgaatgcgtt tgtcgcgctt gaaccattcg
     9961 agagtaattc ccacgagcta ttgattcccc agtcagaaaa agggccattg gctggtatcc
    10021 catttccttt aaaaatgctc ggtcaagaaa aaaagggatg gctagctact tcaggatctc
    10081 gtttatttga aacacaccga gcttctcata catctaatta tgtgcggcaa gcagaagcga
    10141 taggtctagt tccttttgga caaaccaatg cgccagagtt tggtttcaaa aatattacag
    10201 acccagtcat ctatggccca gcaagaaacc cttggaacct tgaatgtaca ccgggcggat
    10261 caagtggagg agctgctgct gcagtttcat caggaatcgt tcctttagct ggtgctagtg
    10321 atggcggtgg ttctattaga atacccgctt cgttttgcgg attgattggg ctgaaacctt
    10381 cgcgagggac gatgccagtt ggtccatatg cttggagagg atggcaagga gctgctattg
    10441 atttcggctt gacagtttct atgagagaca cggaggcttt attttatggg atgagaagta
    10501 tccattccgg tgcgccatat caagtatcgc cagttgagtg gcaaacacat ccaagaaaaa
    10561 aacggttgaa gatagctttt tgtgttgatt caccgatcca ctcacagatt agtgaagagg
    10621 cagtacttgc tgtaaaaaaa gctgtagatt tccttggaaa agaagggcat gatttgacag
    10681 agattcctta cccactagat ggtcgtaaac tgatcacgtc ttattatcag atgaatggcg
    10741 cagaaacagc agcaatgatc cgctcgattg aaacaggcat ccagcgagct gtcagaaaag
    10801 aagaacttga acccatttct tggactttgt tccaatatgg tcagaagctc tctgccgcta
    10861 cttacatcga ttctctgcat gtatgggatg aagcagcaat tgtcatggaa aacctacatc
    10921 aagaatacga tctattcctt tcaccaacga ctgcatttac cgcccctaaa atcagcgaag
    10981 atttgcaaag tgatgcgata agagcacaga tggaacacat cagtgattgt tctgaagtag
    11041 aaggtttaga cgttgtttct tccatgtttg aggagagctt gaagatcaca ccttatacac
    11101 agttggctaa tctgactgga caaccagcaa tcagcttgcc tactcatttg acaaaagaaa
    11161 acatgcctct aggaatccaa tttatggctg ccagaggaag agaagatttg ctatttcaaa
    11221 tcggctatct atttgaagca aatcatcagt tccatttacc aaatccatgg aaatagcaga
    11281 aacaaggaga agaagaatga agaaaaaaag attgtttgct ctaataacta gtgccttttt
    11341 tattttaagc gcttgcggta cagctgccgc taatcaggaa gataacagct ggagtaatgt
    11401 agaaaaaaag aaagaattag tagtagcgac ttctggcaca ctgtttcccg catcttatta
    11461 taatgatcaa aatgaattag ttggttatga tgtagatttg gcaaaagagg tggctaaacg
    11521 gttaaatgtc aaaatctcct ttaaagaata caacgtggat ggacaaatca cttctgtttc
    11581 aaaaagagaa tcagattttg ctgcgaacga ttttggtatc acaaaagaaa gagaaaaaaa
    11641 attctccctt tcagagactt tcaaatattc atttgacagt atgatcgtac gaaaaaaaga
    11701 tgattcagga atccactcac ttgaagatct taaagggaaa aaagcagctg gcgaacccaa
    11761 tactagctat atgcgtttag cggaaaaaca cggagctaaa ttagtcacat atgacaatgc
    11821 cacgaatgat cagtatttaa gcgatgtagc aaatggtcga acagatgtta ttctaaatga
    11881 ttattatctg cagaaaatgg ccgtatctgc tttaccagat atccctgtaa aaatactgga
    11941 gaacgtttat ttcaatgaaa acgaaagtgg ttttttattg aaagaaggaa atgatgctct
    12001 gaaaaagaaa atcgatggcg tattaaaaga gatgaaagaa gatgggacga caaagaaaat
    12061 atctgaaaaa tattttcaaa ctgatgtttc agtcaaaccg aaagaaacga tggctgagtc
    12121 ggtgagtgag tgatgtactt tcctctaatt gatttttccc tcatgtggac ttcacttcct
    12181 ttcgtactgc aaggcttggt atacacatta gggattggct tcgtgtcgtt tcttttagga
    12241 aatctggtag ggttgctttt gacagtttta ggtcttttgg attggctgcc tctgaatgta
    12301 tttattcgtt tttatttatc gttctttaga ggcattcccg cactagtcct gctgtttctg
    12361 ttatattttg ggttacctta tcaattaagt gccttaacgg cttcagtcat ctgctttacg
    12421 attaccagca gtgcatttat tggtgaaatc tatcgaggat cactagcagg cgtttcttca
    12481 ggtcagtggg acgctggttt agcttctggt ttttctttct ttggggtatt gaaatatatc
    12541 gttttgccgc aagcttttcg aatctcagta ccggcactca gtaacgtagc tatggatcta
    12601 gtgaaaggta cttcgttagc agcaacaatc acagtgccag aaatatttca aaaagcaaag
    12661 atcataggcg gtcggacatt cgattatttt tctctctata ttttagttgc actgatttat
    12721 tgggtcattt gtatcgggat cgaaaaagtg caacagtatt tagaaaaacg ttctatgcat
    12781 cagtcaatct gaaaaataga aatggtgcta gcgtggctgg caccatttct attttttgtt
    12841 tcttgtatat tctgcctagt attttaaaac catttcatgt aaaatgtaaa tcgaaaatat
    12901 gttcggaggt agaaaacatg aagaccccgc taaccatcga aatagaatcc tcgttatacc
    12961 aatattgcat cgagcaaggc ggcttggttg tagaagaagt cacgatgcct gatgaccaag
    13021 gaattgtaga tacgcttagc tgcttgacaa aatttgatgg aagccgagaa tggcgatgct
    13081 atgagctgaa agtgacaaaa gcagattttc gctctacagc caaactgtca tttgttggac
    13141 attataatta ttttgtttta ccaagtaaac tatatgaaga agtaaaaact gaaatcccta
    13201 caggaatagg tgttttagtg tatcgtccgt acacggttga agaggagatg cctgcctctg
    13261 gtacatttct gattgcgaaa aaagcagcaa agaaagagct tgctgtaccg gaagaagctt
    13321 tgacaaaccg cttcatggct tctttattca gggaagtaag aaaagcaaag caaatggagc
    13381 gaggcgtaaa atattttcca acagatcaat tgtacaaaga attgaaaaga cgttcagaaa
    13441 atcaagatcc attttctaac gaacgatatt atgaacggtt tttagaggaa atagctagtc
    13501 aggcaatcaa tgagttgcaa gaagaagtta tcgcgcttca gcatgattat gaatatttga
    13561 ggcaacaaca gcagatccgt cggttgccta cagagccatt ggagtgaaaa atatgtacta
    13621 tccttattta agaggaaaac aatttgacct attagcatta aaagaagcac tgtcgcgagg
    13681 attgctctcc aataaaatcc aaccagtgat cgaaccagta cgtgattccg ctacgcttaa
    13741 aaatgtgatc gaattatttc aaaaaaagca ccatccaatc gctgtgatcc aaaatcccca
    13801 agtaggacaa ttcaaattat tcgatcagca tgtgcacagt tggacagtca aagaaaatag
    13861 ctctgtcgtc cctgcacaga tcttaacgcc agaaaatctc agtgaagtac tggattcacc
    13921 tccagcgttt cttgttttcg atggacagca ctacccaaaa gatacagagg tgtggaaaca
    13981 gttggcagga gttgattcga aatttttgat accagatact agtcgctttc gcatctggct
    14041 gcccgaaaac aaaattgtga tccgtgactc ttttcaaacg agaaagcatg ttgaaagtta
    14101 tgcagataaa acagatgact tttttagtga tgactatctt tttttccacg cagacggata
    14161 tattggattt tctgatttca cgatcgaagg tagccgttat ttcgataaag ggttccctag
    14221 ccgagcgctt gctatccatc taacgtatat tgatgcttac ggcaatatac gcgtgaaaca
    14281 ttttgtctct gacagtaacg actctgctaa agaccaagca ttgaagtttc ttgaagcagg
    14341 agataaaatg aaaaaatgga tcatgcgtca ccatcatcaa ctcttgatta cttcaggaat
    14401 gattgaatta ttggctcttt accagcaaca aaaattccct ggactgggag tgttgaaaaa
    14461 gtggtcactt atgcatcatt tggaacttat tagtcacctg ctagatcatc caaaagattg
    14521 gcttaaatat tattcaaaag ttcaggaact atatgaagtg atccaatgat tagataatgt
    14581 aaatgaaaaa ggaatagaaa agaggaaact ggatgtatat tcggcaaaca aggaaagaag
    14641 aagtagaaga agtggtagaa atcattaatg ccgcagcgga tttcattgct tcgcaaggct
    14701 ctcctcaatg gcaaaacggg cagaaaccaa caaaagaaac gattttgaac gatattgaaa
    14761 gaaaagaaag ctatgtatta atagtggaag atcaaattgc tggaacagct gcattagtct
    14821 caagcgtgga tcccgtttac acaaaaatag atggtagttg gaaagaatca actggtcctt
    14881 atttatcgat tcatcgtgtc gctttagcag aaagtactcg aggaaaaaaa ctagcaaaac
    14941 cgttcttaga acatttgttg acgattgcag ccagcaaaaa tatccaagat atccgtatcg
    15001 atacttatcc agctaatgaa ccgatggaaa agaccatcta ttctgctgga ttcacttatc
    15061 aagggatgat tgaatttcct tttgaacacg gcgaaagaaa agcatatcaa ataatcatca
    15121 attgactata aaatcatctg agcaaagata aagacattaa cactactgaa aaaaacaatc
    15181 atatgatagc caagaggttg taacagaagt ggccagcttc aaaaaacaag aaagaatttc
    15241 cgaaaatgct tctcaaattt ttgtggaatt ttagcgtatt ttacgaaata gctgctttct
    15301 gaaactccct tcatcgagag gtcgtgacat agcttttgtc acgacctctc ggcttatcct
    15361 acgagtatta ttcgatacat ttatatcaat aatccttttt ttattttatc tgcagc
//
LOCUS       DACDSY010000068        15041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDSY010000068 DACDSY010000000
VERSION     DACDSY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.068"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            8..81
                     /locus_tag="IWK44_001690"
     tRNA            8..81
                     /locus_tag="IWK44_001690"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="IWK44_001691"
     tRNA            101..173
                     /locus_tag="IWK44_001691"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="IWK44_001692"
     tRNA            201..274
                     /locus_tag="IWK44_001692"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="IWK44_001693"
     tRNA            288..361
                     /locus_tag="IWK44_001693"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="IWK44_001694"
     tRNA            385..474
                     /locus_tag="IWK44_001694"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="IWK44_001695"
     tRNA            487..560
                     /locus_tag="IWK44_001695"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="IWK44_001696"
     tRNA            579..654
                     /locus_tag="IWK44_001696"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="IWK44_001697"
     tRNA            656..726
                     /locus_tag="IWK44_001697"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="IWK44_001698"
     tRNA            740..813
                     /locus_tag="IWK44_001698"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="IWK44_001699"
     tRNA            838..925
                     /locus_tag="IWK44_001699"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="IWK44_001700"
     tRNA            947..1018
                     /locus_tag="IWK44_001700"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="IWK44_001701"
     tRNA            1041..1130
                     /locus_tag="IWK44_001701"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="IWK44_001702"
     tRNA            1143..1216
                     /locus_tag="IWK44_001702"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="IWK44_001703"
     tRNA            1235..1307
                     /locus_tag="IWK44_001703"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="IWK44_001704"
     tRNA            1311..1383
                     /locus_tag="IWK44_001704"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="IWK44_001705"
     tRNA            1388..1458
                     /locus_tag="IWK44_001705"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="IWK44_001706"
     tRNA            1471..1544
                     /locus_tag="IWK44_001706"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="IWK44_001707"
     tRNA            1556..1627
                     /locus_tag="IWK44_001707"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     gene            1893..3086
                     /locus_tag="IWK44_001708"
     CDS             1893..3086
                     /locus_tag="IWK44_001708"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP9550217.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="IWK44_001709"
     CDS             3216..4688
                     /locus_tag="IWK44_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9550218.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="IWK44_001710"
     CDS             4771..5559
                     /locus_tag="IWK44_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP9550219.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="IWK44_001711"
     CDS             complement(5626..6294)
                     /locus_tag="IWK44_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9550220.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="IWK44_001712"
     CDS             6409..8121
                     /locus_tag="IWK44_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9550221.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9893
                     /locus_tag="IWK44_001713"
     CDS             8124..9893
                     /locus_tag="IWK44_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9550222.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10098..10664)
                     /locus_tag="IWK44_001714"
     CDS             complement(10098..10664)
                     /locus_tag="IWK44_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9550223.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(10664..11620)
                     /locus_tag="IWK44_001715"
     CDS             complement(10664..11620)
                     /locus_tag="IWK44_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP9550224.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(11620..12276)
                     /locus_tag="IWK44_001716"
     CDS             complement(11620..12276)
                     /locus_tag="IWK44_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9550225.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            12646..14664
                     /locus_tag="IWK44_001717"
     CDS             12646..14664
                     /locus_tag="IWK44_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9550226.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
BASE COUNT         4562 a         2788 c         3271 g         4420 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa taaagaggtt
     9901 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
     9961 ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag
    10021 gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta
    10081 tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc
    10141 ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa
    10201 aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg
    10261 atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc
    10321 tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct
    10381 gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac
    10441 aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct
    10501 tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa
    10561 accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt
    10621 tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt
    10681 tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat
    10741 tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat
    10801 cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga
    10861 cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca
    10921 ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta
    10981 aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga
    11041 tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag
    11101 tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc
    11161 caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat
    11221 ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta
    11281 cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga
    11341 aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct
    11401 ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac
    11461 tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc
    11521 catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag
    11581 aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta
    11641 agagttatct atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga
    11701 aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg
    11761 gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt
    11821 tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc
    11881 tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg
    11941 acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa
    12001 tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc
    12061 acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta
    12121 gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga
    12181 aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa
    12241 gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga
    12301 cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga
    12361 ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt
    12421 ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa tccttttaaa
    12481 caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt
    12541 acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa
    12601 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
    12661 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
    12721 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
    12781 cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac agcggcattt
    12841 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
    12901 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
    12961 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
    13021 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
    13081 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
    13141 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
    13201 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
    13261 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
    13321 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
    13381 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
    13441 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
    13501 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
    13561 tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac
    13621 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
    13681 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
    13741 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
    13801 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
    13861 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
    13921 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
    13981 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
    14041 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
    14101 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
    14161 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
    14221 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
    14281 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
    14341 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
    14401 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
    14461 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
    14521 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
    14581 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
    14641 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
    14701 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
    14761 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
    14821 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
    14881 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
    14941 aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc
    15001 aaaaggaaat aaaaataaac agattctgcc ggttttttcg t
//
LOCUS       DACDSY010000069        14700 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDSY010000069 DACDSY010000000
VERSION     DACDSY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.069"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            9..641
                     /locus_tag="IWK44_001718"
     CDS             9..641
                     /locus_tag="IWK44_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP9550227.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            1229..2668
                     /locus_tag="IWK44_001719"
     CDS             1229..2668
                     /locus_tag="IWK44_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9550228.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2963..3181
                     /locus_tag="IWK44_001720"
                     /pseudo
     CDS             2963..3181
                     /locus_tag="IWK44_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3430..3645
                     /locus_tag="IWK44_001721"
     CDS             3430..3645
                     /locus_tag="IWK44_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550229.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3862..4118)
                     /locus_tag="IWK44_001722"
                     /pseudo
     CDS             complement(3862..4118)
                     /locus_tag="IWK44_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(4305..5216)
                     /locus_tag="IWK44_001723"
     CDS             complement(4305..5216)
                     /locus_tag="IWK44_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9550230.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(5235..6239)
                     /locus_tag="IWK44_001724"
     CDS             complement(5235..6239)
                     /locus_tag="IWK44_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9550231.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(6236..8359)
                     /locus_tag="IWK44_001725"
     CDS             complement(6236..8359)
                     /locus_tag="IWK44_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9550232.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(8364..10811)
                     /locus_tag="IWK44_001726"
     CDS             complement(8364..10811)
                     /locus_tag="IWK44_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9550233.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(10798..11187)
                     /locus_tag="IWK44_001727"
     CDS             complement(10798..11187)
                     /locus_tag="IWK44_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9550234.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(11247..11750)
                     /locus_tag="IWK44_001728"
     CDS             complement(11247..11750)
                     /locus_tag="IWK44_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9550235.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(11763..11987)
                     /locus_tag="IWK44_001729"
     CDS             complement(11763..11987)
                     /locus_tag="IWK44_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550236.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(12091..14001)
                     /gene="ltrA"
                     /locus_tag="IWK44_001730"
     CDS             complement(12091..14001)
                     /gene="ltrA"
                     /locus_tag="IWK44_001730"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9550237.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         4250 a         2982 c         2605 g         4863 t
ORIGIN      
        1 taggtgcagt gaaggaaaga tatggtcacc ttactgttgt tgaggagatt actcataatg
       61 gatataaaaa atatcgttgc ctatgtgact gtgggaatga aacgattgta tatcgatcaa
      121 acttactatc aggtagaacc aaaagttgtg gatgtggaaa ttataaaaat aggcataaat
      181 ataaaaattt gacaaataaa acttttggta agttagttgc aatcaagcca actaaactgc
      241 gaagtgaagg aactgttgtt tgggaatgtc aatgtctgtg tgggagaaaa aagttagtat
      301 cggcaaagcg acttctaaaa ggtgatgtaa aaagctgtgg atgtttgaaa tcaaaaaagc
      361 atcacattga aaataaaaaa ttcggattac ttactgcaaa gtatcctttg gacaataact
      421 gttcatggaa aacaaaatgg gtttgtcatt gtgagtgtgg cagtatgtgt gagataagtt
      481 atagtaactt attttttgga catactcaaa gttgtggatg tttaaaaagg caagaaacaa
      541 ggacatgtgt tgaagggaca gtaatagagt gtctaacgtc aaaattaccg aaaaataata
      601 caagtggtgt taagggagtt tatcgttcta aagagaaata gattgcgtat ataactttaa
      661 aaaaaaagag gtactattta ggatcgtatg attctattaa gagtgcagct caagctagaa
      721 aagatgctga aaataaattg tttcaacctt tgattgacaa atatcgaggt aatcattaat
      781 tattgtggat aataattaac agtgatatat aaacatctat aatatattat tcatactatt
      841 gatatttagt ataatcctat ggaattgaat atcatagcaa tgtatttgaa aagcaaattg
      901 gaaaacaaat aggtgcaaca ccataaatat gaaaaaattg ggaaaaagta gaatggaaat
      961 ttgcttacgt ctcacgagat agctcacact attaatctga agaacaatga agtcgacttt
     1021 tagaaattgc tagaagaaag taaagcgaat tatcactagt tactatagag taagttcaaa
     1081 taaagcatct ccaacttaca tggttacatg aggatatcaa atcgaaatta ctcaagcttc
     1141 ctatggctag agtcgcgaga agttgttggg cagtagacat aggttatatt gttcttagct
     1201 acacgatcat tagaaaggaa atcctattat gtatagttat caagaagatg caaaagtcca
     1261 aaaaaatcgc tggtggattt tgattgctat atctatgttt accttcatgt ccacattgga
     1321 tggcagtatt gtcaatattg cactacctac catctcagat gatatgggca taccaatgaa
     1381 tcaagcggaa tgggtagtgt ccgtttatct gatgattgtc tgttcttgtt tgttgctgtt
     1441 tggtaaaatt ggcgatagcc gtgggaaaat caaagtattt cggattggta cgctgatctt
     1501 tgtcttggga tcgctgttgt gtgggttcaa tcattcgcta ggtattttat tgttcgcacg
     1561 ggtggtacaa gccattggtg ctagtatgac tatggctaca aatgccggaa tcatcacgga
     1621 agtctttccc atacatgaac gtggtcgggc attgggagca ataggtgcgt ttgtttcatt
     1681 aggctcgatt gctggaccag gtcttggcgg cttgattttg tcccaatttt cttgggcgta
     1741 cattttttgg atcaatgtac cggtgggaat cgtaacgatg ttgattgctg aaaaattctt
     1801 accaaaagat atcaccatga gcggccatac aatcgatgga gggggctttg ctgccattgc
     1861 ggctacgatt atcagctttt ttggagcaat ctttttgggg caagaaagtg gctttttcca
     1921 tccagtcccc cttgtactgt tcgtaatttc agtgattgcc tttgtcagct ttttaaaagt
     1981 tgaaaagaaa gcagcctctc cattgatttc tttcggtatt tttaagaaca agatttttac
     2041 catgagcttg attacagcct tgttgatttt ttcttccaac ttctttgtca atgtagtgat
     2101 tcctttttat ctgcaaaatg ctcgcgggtt gagtcctagc tatgctgggc tgttgatgat
     2161 ggtctttcct ttgatgatgg tcgtcggggc gcctttaagc ggctacctga ccgaccgggt
     2221 tggcccggag attttggtat tagtaggctt gggcttattg tcggtaaccc aggtcatgta
     2281 tatgtttttg tcagtggact ctcctttgtg gctatacatt gtcgctactg ccatcatggg
     2341 tgccggcaat gccttgttcc agtcccccaa taatacgatg gttatgagct cggtcactat
     2401 cgaaaacttg ggactggcag gctcgatgaa ttcctttgcc cgaaatttgg ggatggtcag
     2461 cgggatttcg ctttctacga cggtcttata tcaagcgatg tctgctaaga tgggccagca
     2521 agtaactaac tatatcgcaa atcggccgga tgtcttttta tatggaatga agattacctt
     2581 tttaggcagc tttgttttgt gttttgttgc ctttttcctg actatctggc gtatcaaaaa
     2641 acaaggaggt attcagaaaa atgtctgaaa aaaccactgt tgaagaattt ttggaggcct
     2701 gcgtatgggt gagtgggatt tccatggatg atggaagatg aagtgaactt ctaaaagccg
     2761 aaaaactcaa gtattatctt caaaatacta gtgctcaatg agatgcttga tttgatcaca
     2821 agaagactgc gaaagccgct ggcaaccgca agctaggtat cccaccgatg gactacgttt
     2881 tagaggctac tggttatgaa tacggagcca acgctctcat ctgcataaga atacatcacc
     2941 cagattatgt aaagattttc gatacttctg tcaaaaaatc attaagatag ataaatgatt
     3001 tccgtctagt caaattggtt cagaatgtag acacacagat ggtaagaagt cactcacaat
     3061 tcgagaatgg acttgtccta tttgccgtat acatcataat cgaaacgtca acgatagtat
     3121 caatattttg accaagggct atgaatacaa gcattgcctt agaaaacatc aagaacctta
     3181 ggaacaacgg ggatagcttg gttaataaga gataccgctg tttaggcttg caagtatact
     3241 cttttatcaa gaattccgtg gctttagtca tgagaggctt aatactatga aaattttctt
     3301 tagcattttt gatttaggtt ccttttctaa ttataataag atgaccacta cagtaaccta
     3361 ctgaaatata taaacagcca ctgtagtacc attttcaaag tagtctatga gaggagaatc
     3421 ttcattctta tgaacgctgg aattgcggat atgaatttaa taaaaaaaac ccttaatgat
     3481 tttacttcca attcaatatc aaaaggtact ggtatcaatt taagcacaat aaaaaagctg
     3541 aaaagtggtg aaagatcagt ggaaaagctg aatcttttag atgctataaa aataacggaa
     3601 tttgctatga aaaatggaaa agctgagatt gaaatatgga gataattatc gtctagtgaa
     3661 ttacttgcca cttagctgaa tttaacgatc cttggtgctt gaagggggag attcttggga
     3721 atagagcata cttgcaagcc tatgtcttta ctaacaacga tatctcttat taaccaagct
     3781 atccccgtag ttctaaggct tttactttta tctaagcaat aatcgtattc aaaggaaatt
     3841 tttgaccctt agaaagtctc attttatcag acgtgagctt aggcattgct gaattttgtt
     3901 aattcggttt taggttgtgc tttgagcata atatgaaagt attctttatc acgatttcat
     3961 tcatctaagg taatgtgatg tgattctgaa attctttcaa aggcagtttt tgcataagct
     4021 gacattacat catcaatgac atgtttacaa tgtttcacta ctaaaataag ggggtaacca
     4081 agaagaaaaa ctgaatgatt atttgtatct aattccatgg tatatcaacc atttcctata
     4141 tagactgatt atgacatgga ttaaaataaa aaggcactat gtcttaattg tcggttttcg
     4201 agttaccgaa tggcattcat cacccaccta tagaggatgg gcgacttctg cctgaaataa
     4261 gttacactac ttataacttc taataaaatt agattgagtc attattattt aacatcaacg
     4321 attttccagt ttccattttt ttgtaatact aatttgtact gaaagtagct agtggttttt
     4381 gatacattat caaggtattt tatctttgta ctcactagaa aattttttcc gttttgtaga
     4441 taaactggat tctctaactc cgaaaacttt aagtcaatat tgattggtcg catcgcacca
     4501 ttttcaacat aatactccaa ttctttttca gaagctgatg gataaagttt aaaaaatgtt
     4561 tctaaaaaat cggtgatttc ttccgttgtt ttggcatcaa tttctgaatc gttttctact
     4621 tgttttgctt catattttgc cttgtctggc tttccaatca ttgttggcaa actagttact
     4681 aaatgattcc tgttttcatc ttcaaagatt gtcactcgat atgcagaaga aattgattgc
     4741 gtcttttttc cctctgtaat atgttgctcc actaaaaatg aaacgacaaa ttcttccgag
     4801 tgtttttcaa catctagaat ttttacagat tgaacttcgg aactcgtggg aatatctgct
     4861 cgaaccatat cttggattaa agcgagaccg ttttctgtaa ggtactgttc gagattactc
     4921 attctctttt caattgcttc tttgttattt ttccatgaaa aatattcttt cgcaaaatcc
     4981 tctgtaaaac tttcaacgcc agaagtattc actaccttct cttttataac cactttttca
     5041 tgagttgtat gttgatcaat ggcggtgaaa tttttataaa cgccaaaagc aagactacaa
     5101 gacaataaaa gccaaagggc aagcaccatt tttcgatgct tacccacgga tacaactcgt
     5161 tcttttttct tttttgggct cttttccttt tgattccgtt caatttttat cttcattctt
     5221 tttcctcttt tctcttattt gtatcggcca gctccgataa gatgctgccg ccaatagttt
     5281 tccgttagat tcgtccaccc cactggatct cctgcatgaa acatgcgatt ctcccctgcg
     5341 taaatgccca catgggtcac ataagtaccg gcatcatagg ttgaatgaaa gaaaactaaa
     5401 tcacctggtt tggcatcttt tagatcaata tgagtcatgg catcatattg atcctgagcc
     5461 acccgaggaa gtttcaggtc aactttggca taacaccatt gggtcaagcc actacaatca
     5521 aaccccgttt ctggacttga gccaccaaac acataggccg ttccttcata cttaaatgcc
     5581 tcatcaaaaa tttcttggac tattttgtca tcaaactgag ttgtagttag gtattgcttc
     5641 actaataaac agtaaaacat attgccataa ttataccgcc acccaccatt ttccttaatc
     5701 gcgattgggt tggggtaatc gactttttga ccacctgatt tttcctttga aaaagattca
     5761 gctaaagcaa aactatgctt cttcccgttt ttcccgacat gatctagata cccgccacca
     5821 tagttatagg cctgaataat actatcttga tcacacccta gttctttgga acgcttaacc
     5881 aaagacgcaa aatagagaca gccttgtttg atggatgcct cggtacttaa tgaattaggt
     5941 ggtaatccgg ctgactcaga actttgcata acgtcttcca ttgtgccacc agattccact
     6001 tgaataatcg ccaaaagaat tggaatatac tctgttatat ccgattcttt ggcatacttt
     6061 tccaccattg cacggtgttt caatacttct tctgagacac tagttccgcc aggagaagtg
     6121 ccagaactac catttccctc actgtcttca ccaaacaaca aacccacaca catcaaaata
     6181 cccataaaag agatggtcag aaaagttaat gaaataacac ccactttctt caagttcatt
     6241 ttttctcacc tgaccttgat tttggacgat ggttcattct tttttgtact ttttgattgg
     6301 tctgctttgg cagagtggac ctctgtttca cttgttggaa agaatttttc ttacttgctg
     6361 attggctttt cccttttgaa atgatttctt gactagttag ttttcgtttg atttcgggat
     6421 tcagatttgc tctcacaatc cgctttggtg cttctttttt cagcatcata tctttctgag
     6481 gttgagctgc tttatctatt ggaatattcg cccttctttt tccctgacga tttgttgctt
     6541 cactcagtat cttgcgtttt tcgtccattt tcgcatttct tcgttgtcgt tgtttttctc
     6601 gcaaatccac tcgatccgct ttttcttgga acagtccgcg cttgaattct tctggtgctt
     6661 ttttcgactt ttcgattcct ttatggagat tgtatttcaa attcgttggc gtattcctta
     6721 cctgatcctt tacttgttta gtcttatctt tcaccaaagc ttgggtatcc aaaactttgc
     6781 cgatttttct cccagccaaa ttcattcggg attgtttctt attatttgaa actggattct
     6841 ccttagttaa gtcagatagt atttcgtgat cgttcggtaa tcgtgaattc tttcgtagag
     6901 aagaaccgga tggcttaaat ttggattttg attgttcctt tgccaactta tttccgactg
     6961 ctgcaccacc accaaaagcc aaagctcgtc ccacatttcg attgagttgc cgcaattttc
     7021 gattcattaa catttgcggt tttcgcatga cgcgtcttcc cattgactgg ctatcattac
     7081 tctggaggtt gaacatactc ataatgtcgc ccaatttgaa atagataccg gcaaaggtca
     7141 caatttgcag aaaagcaacc atgaaaaatg gataggttgc cgaaatattg aaaaacatcg
     7201 tggagatgct aaatgcagtc gtaatcacca aagtaacacc cgctcgcatc ataatcgtat
     7261 tgaataaacg aataatcgcc ttctttccta aactttcata agtcggcaac atggacaata
     7321 agaaactaat tggcagaaac atcgcaaaga taatgaagag aatttgcgag aacaacataa
     7381 tccctgttag aagaaaaaca aagaaggaaa tgccaatatt gaacaatacc aaaaagacca
     7441 cggtccctaa acggttcatg gtcttggtaa tactgagatt cgcattgtca ttgtcttcaa
     7501 tctctgcctt gaccgcttct tcccgatctt tgcctttgtt ttcatctgga ctcacagata
     7561 gaatcttgtt gacccggtct tcaccaattt cttctatgtt ggaatcatca aattgcagta
     7621 gtaaccaagg ttgttggact tgaatcgaaa acaaactatc ccgaatcaaa tccacactat
     7681 cttttccttg gctttctgaa ttgggtacaa cgatctcggt tccaagggta agggccgctt
     7741 cactaacatc tgagctaaag tcattgattt ttgtgatata ggtaggcgca taagcaatga
     7801 acgaacccga tagtagaaaa atcatcacaa aattaatcac ggctcgaact gctttcgtgg
     7861 tttctcgttt taataatccg gtatacgcca catacacccc cagagctaaa atcatcaata
     7921 atagaaaccc aaaataaaat ccggaggtct ggagtccatt ttcagtaata ccagccaatg
     7981 tttggatatt ctttccaata ctttcagccg tatccgaaat gaaatctaat ttgtaagcct
     8041 cttgaaccac ataacccgtg gcgttcgaca aatacaaact caccgtccaa atgaaattag
     8101 taatggcata aagaccatac atcacgcttt tccctaagcc atcaccccaa ttccaaggca
     8161 accaatccca agagctatcc acaaagaaat ctagttgata gttgttcagt gaatatttgg
     8221 aatatagatt tcctgcttca actgtatcat caactagccc tgtcgcctcg gctaccgtac
     8281 caactaaact caatagaact atcagagtga gtatgctaat aaaaacgatt cccacaatcc
     8341 gaaaaagttt ctttttcatc ccctcactcc attctctctt gaacaggtgg acgggtatca
     8401 aaggcatgga agagttcttc aaagactgga tgtacctgaa ccacgcctac ccgaccatac
     8461 aaatcctgca tcaagcattg gccattttcc aaatctcgta ggcgtttttg attgctttca
     8521 tcttctcgat ccaagccaaa gaattccaag gttttcttga tttcatgaat atcggtactg
     8581 cgaaacgcaa acttcaaccc gatattattc ttcaagcgtt catccagtaa atcatcacta
     8641 ttttgcgtga caaagtaaac cccggcattc atagcccgcc cagcacgaac gagcttattc
     8701 gataatgttt tcccttgcgc tacttgaaga aaactccacg cctcatctaa gtctacaatt
     8761 ttgaagattt ctcgattact atggataaag tccaaggcaa aagtggaaat gacgattagc
     8821 atggaaacac taagcaattc catggtggta tattcttcaa aagtggtttc tgcatctggt
     8881 aaaaccaaat ccgctacttg aatgatattt aactgcctat ctaaactaat cgactgggta
     8941 atcgaaccat ctgagaaaag caaatgggca aaatcataat cggtaaagga ctcgatgtga
     9001 tccgcgatat tttccgcaac tggtgagcct tcttttcgaa gctcattaat tacttgtaat
     9061 aatccccgtt gattgctttg ggtaaccgtc cgaatggcct tacgcaacac tggaaacttc
     9121 tccccatctc gactagaaat tcctgttaaa aaggtcagaa tatcaattgc tagactttca
     9181 gaatccttct ttttctccat aatcacataa ggatcaagta atccttgatt ttttggctta
     9241 ctagttaagt tgagaatatt gatttcatcc gcaatttctg gcaaggtttc tttccagcca
     9301 ccccgttccc cttttggatc gacaatcaca gcttgtccac caaaaaggac ggcgtaataa
     9361 actaagagat tgttactaaa ggactttccg ccaccgagtg aaccgaggaa tgccgctgct
     9421 agcgcattgg taactgaatc cttgacccct tgtgaggcca atgccggttt caggtaaaca
     9481 ttgcgccccg tatctacgtt ataaccaaag taaatgcctt ccaactcccc taacatctga
     9541 gtggcgccaa aaccaagtcc ggcgagaaaa tcagacgtaa catactggat ataatcattc
     9601 atgtatcgct tactagaagg taagaattct tcatgtagcc ccatcatatc gccaaaaggc
     9661 cggactagtt ttacattggt atcatcataa aaatctagca cttcatcaca ccggcgcttt
     9721 agttcatcta ctgattcggc acttactcga accacatagc tcagtttgta catggattct
     9781 ttggactgat ccaaggtagt ttccaattca tctacggaat ccaatgcatc taagacattg
     9841 gaatttgttt cattgtcaga ttgataggca tggttgtcca agtcttttaa ttcctttttc
     9901 ttattgcgaa cggtagcaag tgcctttttg ttcgtcacaa tctccacatt catactagtg
     9961 tcaattggga atgtaaattg ttgttgctgg taatagaaaa gttctgagga gggaaactcc
    10021 atttccccca caattgtatt aattgttaag tacgctacat atgattcatg attctcatgt
    10081 tccatgcata agtaccgggg cttttcttca atcaaacaac gactgggacg taataagtcg
    10141 taacgtttca ccaaagtttc cgacttcagc tttttctttg gtagctgaaa ttcatattct
    10201 tcaaatggtg tacccttttc gccatagata tgttcaatca aatacatcag atcactcggt
    10261 gtgactcgtc tgactttaaa tcttcgagcc aatttacttt ccattagttt ttcaagcttg
    10321 gtatatcggc gaatttcttc attggataag gaaacaaaat cgcccatcaa atgatgattc
    10381 actccataga caaattcttg aaagccggag aagaaagatt tcttgagatt ctttagattg
    10441 acctcttcat ctgtcgcaat cagtttgaag ccaataaaaa agcgataatc aatctgacta
    10501 tcgccaatca tggatactaa tgcctctgtc tgtaaatcaa ttcgttgttt cgctacttct
    10561 gttaatcgcc cggtaatttc cttttttgat cgttcttgcg tggcacgaat actactttct
    10621 gtggcaattt gtaaggcatg aatcttgcct tcacgatttt gcgcaatcaa ttgacgaaag
    10681 ttatcgtgga cttgatattt ctgttcaggt gacaaaaagg agtaattgta aggtattaac
    10741 tcatagtagg caaaacattc tccctcttga ttaaagacaa gattattttc aatatactta
    10801 atcgggtaca tagttcacac tccttaccag agtaatgggt tcgtcccatc gtttcttctg
    10861 ttctttgact ttctttcctg cgaaagtgac cttaaatcgt aagtgatagc taataaaaga
    10921 tcgtaaaaat cccaatggtt tcttaccgtc aaacgttttt tgcgacatga accaagtaac
    10981 tgccaccggt atacctaagt attttaaaaa ggccccctct atcatagata gtggaggcac
    11041 atgggcaaat acgatgacaa acagcaagga taaaacaaac caagccatct ggttaaaagt
    11101 cacaggaaaa ggcagttgaa agtcattaat tgcataaatg accttttcaa ctgcccaaat
    11161 actggtgtag ctttttattt tcttcatcaa atcattcctt taaaaagaag gatagtttag
    11221 aaatcaacta cccttcaata attttgttaa tatggatact caaaaacacc tcgactcgtt
    11281 accaaataat tgcctccaat ctccaagtct ctaccacacg catcaaaatc aatatacgtc
    11341 ttcaagtgtt ctggtacttc tccaaaatca ccagcttcta caatcaagaa atatgctaca
    11401 tcactcatat catcgcaatc tggataatag ataatatcat ccacatgctc taccatctcc
    11461 tcaaaagaat tgaagaaagc atgttgaatc tcagaagcaa cttcaccaac tggcgttcct
    11521 tcaagttcct ccgctaagcc acacaaccga ttaatttcct caattggcgt atactcatca
    11581 atctcaaagg gaagttcata atcatgaatt gcatattcct cgtattcgtc atttaaccca
    11641 attcgttctt tcacttcatc aaaatcgaca ggtggggtaa accacgcccc caccaattca
    11701 ccttcgttat atttcccgag gttcgcaata taaacacgca tctgttccat cgtgcttccc
    11761 ccttacgcac cgacaattcg gttaaacaaa ctgagtaaga catccttcac cccagaagca
    11821 ttaaagacca atccgaccgc aatcaaagca atcaccaaaa agccaatcag tttggaaaac
    11881 tcccgcttaa atcctaaaaa caaaccaatg accacaattg ccagtaagac taaggattgg
    11941 gcattggtta aaaaccattg atatatggat aggtaagttc ctgatattat aaatatctca
    12001 aacttttccc cccattcaca cggagcgtgc gactttcacc gcactccgcg ttccatcgtg
    12061 tttatattga aaagtattta tgtagtagac ctatatcaag tttaattctt gattgccagc
    12121 taatactcta tatttattta gtcttatgag ctggacttta tttagcttca atagagctat
    12181 atatttttgt attgtttctt ctgttttagc gtgtattaga cggtgtaccg cctcttttac
    12241 caaaataaga tttttataat cgtcatttcc accagctcca gttggtacaa tatgatgaca
    12301 atgtatttca ttagcttgtt gaaattcttc cagagttata gcacattttc catgttgagc
    12361 agaaaatagc gaaattcgat tgtctgcgta ttctattgtg cgattttcca aagataattt
    12421 cattaactgg tacatcagta atttatcaac acttagattg gtgtggataa attttcttcc
    12481 ctctagagtg tagcaattcg ctaaatacac ctttgccatt ggttttctgt gttggatata
    12541 tccaacaggg aaaataggtt cgccctcgcc agcaacatac cgaagcattt tagatttacc
    12601 atatcttgtt gcctcctttg tcgtaagttg tctgcctgtt tttctcaatc tgcccctttt
    12661 gtttgtacct aatctgttag tgaagatagc cattacagct ctattcagtt tcatacaatc
    12721 cagatttaca ttcgttgcaa tctgataata attttgcata cctaatacca ttgaattata
    12781 aagtcttgtt tcttctaatt ccgttttccc ttttgaggga gaagcaattt tctttgcctg
    12841 tgataccagc ttgtcttttt ggtttttgaa ttgtttatca cttatatgtg atttaacaac
    12901 taacttcttt cttctcggta tcattttaat cttgaaacca agaaagtcgg agtatctttt
    12961 tctaacattt acaattcttg ttttttcttg tgatacttcc aattttaatc gttgttcaat
    13021 ccattgagtg attgctattt tggctttttc agcactttcc ttgtagcggc agaaaactct
    13081 aaaatcatct gcataacgaa ccatatacat ttcctttaac tttgttttct tcatttcttt
    13141 gtaagcgttg cttttacatg ggctaccgct tttattgata tgaaccacat agttttttgt
    13201 aacaggattt tcctgccatt ggctttctac ccaatggtct aactcattaa gaacaatgtt
    13261 tgctagtagt ggggatatta ttcccccttg cggagttcct ttctctggat atgttattgt
    13321 accgttagtc attttgattg gagcttttaa tatccttttt aaaatgaata tcaagtgctt
    13381 gtcccgtatt cccatagccc atatctgttt tatcagcttg gaatggttca cgttgtcaaa
    13441 gaaaccctta atatcaaatt caatcacata atgtagctgg ctgatttgta ggcggttata
    13501 ggttgctttt attgcatttt caactgacct attaggtcta aaaccatagc tgttttcact
    13561 aaatttagct tcgcagattg gttctaatac ttgttttata cattgttgta taagcctgtc
    13621 ccacatacaa ggaataccta gaggtctggt tttaccattt ggttttggta tttcttttcg
    13681 cctcactggc ttaggtcgat acccatgtgg actgcctgca acaatgtatc ttactttttt
    13741 gacaactgtt tctgctggca atttgccaat atctttaatt attgtaccgt ctgttcctgc
    13801 tgttttactg cctgcatttg ctttgatatt tcgatatgcc agtaatatat tttctctcga
    13861 taggataata tccataagat gagtgaaaat atctccattc ttacttttgg catataaatc
    13921 atcaaaggtg ttttgcattt cgtaatattc agcgtgtctt aaatcttcta cacacaatgt
    13981 ttcattttta tttttaggca taaggcttca ctcctttact tgaaattgag tgagcttttt
    14041 tatcttactc gtaatcctta ttgaaacaac ataatacttt tctaatatat acacgacttg
    14101 agggcattcc tccattctca ttacaagaat ttcttaggtt cgccctctgc ttttcagcat
    14161 tagttcaact gcttattgtt cttcgtcagt ttattctctt gatgagaacc caataccttt
    14221 cctcgttccg ataatcttat ctatacatat atatccttag gtcactagct ataagcctgc
    14281 tggcttgata ccgcctgtaa cgttatatgg catttcataa cctatattct tactttgcca
    14341 caccagctac gatttctcgt acctttgcat ttctacaaag ttcaaattta gacccgtaca
    14401 ttcgctgttt cgtcagttcc ttatagaaca ttctaaccat agatatttta tagacccccg
    14461 acctgttgga tacgctaccc acctctggat agttttcgat agaagatata tgttcatcaa
    14521 ctattacggt atctttcagc cgtctttacc gagcttcaaa cattctgagt ttcctcatac
    14581 aatgcttgtc ggaggctcag ggcaggcgtt tcagagcgtt acttcatcat tccacctgtc
    14641 agattatcag ttctcctaat aaccaaactt tttcgttctt ttattagtgc cttagctttt
//
LOCUS       DACDSY010000070        14542 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDSY010000070 DACDSY010000000
VERSION     DACDSY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.070"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..379)
                     /locus_tag="IWK44_001731"
     CDS             complement(<1..379)
                     /locus_tag="IWK44_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9550238.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPS"
     gene            complement(473..1975)
                     /locus_tag="IWK44_001732"
     CDS             complement(473..1975)
                     /locus_tag="IWK44_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9550239.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYTRGAS"
     gene            complement(2031..4511)
                     /locus_tag="IWK44_001733"
     CDS             complement(2031..4511)
                     /locus_tag="IWK44_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550240.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(4495..4830)
                     /locus_tag="IWK44_001734"
                     /pseudo
     CDS             complement(4495..4830)
                     /locus_tag="IWK44_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4844..5878)
                     /locus_tag="IWK44_001735"
     CDS             complement(4844..5878)
                     /locus_tag="IWK44_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9550241.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(5968..6648)
                     /locus_tag="IWK44_001736"
     CDS             complement(5968..6648)
                     /locus_tag="IWK44_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9550242.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(6932..8035)
                     /locus_tag="IWK44_001737"
     CDS             complement(6932..8035)
                     /locus_tag="IWK44_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9550243.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRAPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(8664..9134)
                     /locus_tag="IWK44_001738"
     CDS             complement(8664..9134)
                     /locus_tag="IWK44_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP9550244.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            9464..11104
                     /locus_tag="IWK44_001739"
     CDS             9464..11104
                     /locus_tag="IWK44_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP9550245.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            11258..12580
                     /locus_tag="IWK44_001740"
     CDS             11258..12580
                     /locus_tag="IWK44_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP9550246.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(12648..13082)
                     /locus_tag="IWK44_001741"
     CDS             complement(12648..13082)
                     /locus_tag="IWK44_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9550247.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(13147..13983)
                     /locus_tag="IWK44_001742"
     CDS             complement(13147..13983)
                     /locus_tag="IWK44_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP9550248.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         4281 a         2930 c         2633 g         4698 t
ORIGIN      
        1 gactaggaaa actatctata attccgacta gttcaccttt ggtgagcttc ttgatgaaaa
       61 agtatcgaat aatcttgata gtgtaagcta agtttgaact taagcgagtg aatcgactcc
      121 tactaggaaa gtcaccatta agaaataaga cggaacaaac cgctctatac gtggctcttt
      181 ggctagtata gccattaatt atgccccata taacacaagc aataatcact gtatcgcgtt
      241 gcttcagttg atcagtattt cttcggtttt taatcttatc tggtacacaa ttgtggtata
      301 tgttagaaac agttctcata atttccttga atgttataaa agtatctgta taatgttcag
      361 tatattttga tgaacgcata tgtagtcctc atttcttggg agttttgaga ctactatatg
      421 cgtttttttg ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag
      481 caccacgggt ataattatat tcgttgctag tgaatacaaa aactttagcg gacttctgtg
      541 acgggtactc tttcaattgg ctagtcaaaa tgatatcata ctgcttttct ttctcgtatt
      601 cgactaaatc atatttgatc gtattcagca attgttcctg gatgatttct ttcacgatat
      661 ccgccattag cctttcataa ggaagatcat aagcaatttg cagacgtaat tctaactgct
      721 tgatcagcgt ccgcaaaata gatgcatagc gagcaaataa ttcaaagcgg gcattttcct
      781 cgtctacttc agtaaaatca acaatagcaa atgtttgtct gaccaatctt tcacataaaa
      841 ccaggaattg tttatcttct acttcagtta accgctgttt taatccttct tggccaaaaa
      901 tattcatcca tcctctaaaa taggcaaaat gagcgtgatt ttgcatcact gtcaacgcaa
      961 tcttttgcga aatctcttct ggaaaaacat aaaaagagaa atattcttct atcgattgtt
     1021 taaacgttcg attcaattga gctactacgt cgtctttgta tttttcgctt atcaaaaact
     1081 cctggacatg cgagttatgt gtatctaatg taaagttact gatcatgaaa agataaaaaa
     1141 ccaacgtttc attttgattc caaggaaagg catattgaaa gaagtaacgg gtcaaaaatg
     1201 tatggaggtc caataaaaat tgtgtcgttt gaaacaatcc aacaggaaga ttgctataat
     1261 ctggtttgga ttttttcgta cagcgcattc gcttcttcat aatggaaagc catatttcaa
     1321 atttcaatcg tccttcttct gtaatagtaa aactaaactc tttttgcagc agatctaaaa
     1381 aagtgtttcc tgataaatca ctgtattctt ttttgttttc aagataagga cgtccaaacc
     1441 aaaataattg gaaataaaaa taacaaattt gactttctgg accggtcagc cgcccgcttt
     1501 ttattttcag atgaaactca tttaaagaat catttaattc cttcactctg cgagacaaag
     1561 tagccgaact gatattaagt tccatgacaa gtttctgccg atcaaatgag ccattttcca
     1621 acagataaag aagtatctga tattttgtag aatagcgcaa gtagcaatga taataaacat
     1681 cgactgctgc tatatttcca tgtctgatta ataaaatatt cccttcttgg ataacaattt
     1741 ccaattgttt gatattttcc aagttagctt gtaaataaaa cagatcttct ttcaaggtag
     1801 cccgatcaac tgataagcgg gtaagtacgt tattaacgga aatctctcct ttttcctcta
     1861 ataaaagttg gaaaagtttg atttgccgtt gttcattttt ttcaagcaaa ctgaaaagtt
     1921 ccaagtccac tcctccaagc atttctgatt ttaacagcag aagtttcctt ctcatccttg
     1981 aaaaagagga tctgtgacag ttatcacaga tccatacaaa ttctaatcac ttagctggtt
     2041 ggcccagctg tcagttccca tgtgaccatg ccttgatacg ttgcgccagg ttgagcattc
     2101 gtactgctat ttacctctaa gagtaaacca tcggtatcgg gaaagtcaac gagaacctct
     2161 cctttagctg ttccatcact ctcataaatc aacgtgtttt gaccttgctg aatcaattgt
     2221 tttgatccag aatgattgaa gtataatttt gttccgctta attcttgacc ttgatcattc
     2281 ttaaataaag aagtcaatgt ccctgtcagt tgccaaggag acgtgcttgg aagccgagta
     2341 tcccgcacat caaaagaaaa atcgcctttt gcttcataat aacttgtctt caacggtttg
     2401 gggagatcgt taccaaatgt aaaatcatct ggtacattaa ctaatgttaa ttcttgtttg
     2461 gggacaagaa ttgttttagc agatgtttgg acaactgggt taggaagctt agcaccatct
     2521 gctaatgtaa tattgctgga aagtacagct gcctttgtca gtatgctgac ggattgtaca
     2581 gaagtatcta cagtatttaa ttgatagttg taagtaagat gaatggtatc tcctattttg
     2641 ctcaatagat ctgctgggac agaaactcct tgttgaatgc cggtatctgc gatagttgta
     2701 ccatttaatt ttaaggaacc attgatataa gtagtatttt caggaagtga gatcgtgatt
     2761 tttttgtcgg ttatcgcatc cccagatttt gttaacgtga atgtcacatt ttcttgaatc
     2821 gtatctcctg atagagcaga caggggatct cctgtctcag gttgtctgct aaggtttttt
     2881 agctgactat ctgcactcac agatggaaaa ttagccactg ttatttgagg aagagtggtg
     2941 attccagctt gttcagactg ccatttgtac gcaaaagacg gatacatgtt agtaagtaca
     3001 tcgtccacca agcgataatc aaaataatat tgccctgaag ccaatgcagg tagtgtagaa
     3061 gtagctgtat ttacaccccc gttatttgat tggatcgttt ggaaaagttg atctgtccat
     3121 cctgtcgcta atttttgggt tcctttcgaa taaagcctgt aatggatcga accctttttt
     3181 agttcagttg ctgaccaatt ggccgtgact tggtacgatg cagaagaaag gagctttccc
     3241 cactgactat cggctgttaa gtttgttggt aaaaccgttc cgtctagctt gtactgctcc
     3301 aataccttta aagcaacagg agaaagcaat ccgccttgcg catctttccc actgtacacg
     3361 atattggctg tctgattcaa tccacttgtg cttgctggag tatgagtcag tattgctcct
     3421 tgtgtaacgt tttttcctgt aaaatagttg actacgcgat caaagcgact aaatggcaac
     3481 tgatcaaaac gtccttcagt cagccccatc gtcaatgtcc gactattggt agagcctgga
     3541 aggttctggt tatttccatc cacgtattct gtggtatcag caaatgtatt atattttcct
     3601 gttgaatcac ttgttgagat gtaactattc cacgtattta tctctgcact tgcagaaaat
     3661 gcatatgctc ctggattcaa gtttccgcca gttaatttag gataaaatct acctgcagct
     3721 ggagctaaaa tcgaattgaa catacgatct tggataatat tattcgaatc ataaaaatca
     3781 acagaaaaaa cggccttttt agacatagaa ggttgtaatt tacccttggt caaaattttg
     3841 tttggactcg tatcatattt atctccgttt tctgtcatct tcgcgacatt agctgtaaca
     3901 tcaaattgaa gcgggatatc gtccacgttg aattcgcctc gcgtaataat agcaataata
     3961 tctgccaata gagtatttgt atcccacgac cccatcccaa aacgcaaggt atggcttcct
     4021 gctgataata aactaccgct agtaaccttg atgggggcac cggcaatata caagtaggcg
     4081 ttgttccagt caattgcttt taatacatca ctgcttgata catgttccgg caaattgatg
     4141 atcgccacaa atgcaaatcc ttgtgcagtg atattcacca aatcaagtac atttgttagt
     4201 ttcacatggc tttcccaatg aaaacggacc gtgccacctt tccaaggatc atttgaacca
     4261 tcgttcaaat aagtagcagt caatttaggc aattggttag ccccagttag ttggacaagc
     4321 ttcccgccat tgactgcact acgtccacca tttggaggta cgatattttc atacgtgtat
     4381 ggatcataag aaatggcagg aggcgttgct ccaacagctt ctgccgaagg tgaaaagata
     4441 aatatactta aaacaaaaag cgaagtaaaa aagaaggagc gaaaaaatga aaatttattt
     4501 tgtagcaaca tgttcatcct ccttttcctt tcttctccat aacaacaacc acgcaagaag
     4561 ccataattct ataccaatga tcaaataaag aaaacggatc ttttcttcat tcgtctccgg
     4621 gaatacgcca tgttgattag attctttata aatcggtagc tgtgtctgct attttgatgc
     4681 gccattgact gaaccacttg gatgcggaac tggacttaga tcataaaatg taatcgtacc
     4741 tgaacttttg gtatctgctt ggactggctc ttgccagctg aagaacagca aacttgcaat
     4801 aatccctccc aataagatcg atgttttcaa cgaaatcacc tcatcatgtt gtttctcctt
     4861 ttttcttttt cttgtggacc cacaaaaaca gcaaaatcaa ccataacact agaactgcag
     4921 caaccaatcc tgcataaagc catgggaaag attgttcttg tactgcaaaa acggaatttt
     4981 catttacttt ttgtgctttt tcttttttga tcgtaaagtt ttgtgtaaat cgccattcat
     5041 tactgtcgcc tgattttgct atgatttctg cgcggtaatc gccggctttt gctttatcac
     5101 cagctaaatc aagattcaag tgaaaattcg aatttggtgc aactaaaaat tcattagacg
     5161 tattttcaat ataaagctga ttttcataaa agatcttgct cgtaaatatc aaattgctga
     5221 taatcctagg ctgaggattt tggaaacgaa gctgaacagt tggctttgcg ttgacctcgt
     5281 ccaaatctcc tgataaaagt ttcagatccg gttctatcgt ctcttgactt tcttgtaaaa
     5341 cgactgcgag attataagca atacgatttt tgatttgtgc tccttctgta gcattttctg
     5401 tttcagcttc ttctttaggt gaaacatgaa tacccgctaa tacacgtccc ttaaagcttt
     5461 ttgctgggat tttcacttct ataggtatag ttgtttctgc atgtgctgat acattgattt
     5521 cctttttcgc aatgagcaaa acgtcacgga catcaaacgg caatgtctca tctaacgttg
     5581 ggtttttttg actataatcg atcgttccac catcactcgt taccgccgga ttgacagaga
     5641 cctcaaatgt tcgttcttca tctgaagaat tgctgataat tacttctagt gtctgatttt
     5701 gttccggtgc cactaataaa tcaaaataac cgattgcttt atctatttgg ttctctggaa
     5761 ataccggttt gattccaaag tctcctgctg aatcctttgc ttggctgatt gacggaaaaa
     5821 tgaaaaatag acttaaaata gaaaaaaacg aaacaaacag tcgtttactt tgattcattg
     5881 tccaccacat cctttcaaca acaactgcgg taaaaaatcc ttcacctata gtattttcac
     5941 cgcagtttgt tgaatttatt tcatactcta tgctactggg ccagccacga ggttccaagt
     6001 caaatcagcc gtgaatgttc cagctgctgc tttcggcact ttcagtgtga tatttgctgg
     6061 atcataaaca gaaagccatg ttcccagacc ttgatctttt gctgctgtaa atattggtgt
     6121 cgctgcactt gctttactca acccagttac agtttgacta gttggtccag tggattcgtt
     6181 atctgacgta cttttaacag tggatgttcc aaaatctaat tcagcgcctt gcaaaacttg
     6241 tgaaccgttt acaaaatctg aaaccgaaac tgtcacgttc cacccttgtg cttggccatc
     6301 tgcacctaca cctcgacgat ctgatacttg gagataaggt gtatcgtttt ttgaagcgtc
     6361 tacttgatac gtttttgttc cgctttcaat ctcgtgtgta ccaaatggca attcaggaac
     6421 tacatccaat gttaaagaac ctgtctggcc agttgctgga ttttccggat ctgctggctc
     6481 tactggtttt gaaggatcag tagggttaag aggctcaacg gtatttgttg gttcagtaaa
     6541 ggtaatatct ccttttgaag tcgcttgacc ctcagcaaaa gctggtgttg ccaagccgcc
     6601 tagaagaagt gttgaaagta ataatgtccc aatcatgttc tttttcataa taaatcactc
     6661 ctttttctta gtgacctaag tatagtagag acagtatgca aaaaatcctc atgaattcat
     6721 ccgcttactt gaaattatga ccctttttgt ataaaagaga aaaaaacaat aaaatcgttt
     6781 acatgaatat tcttcctttt ttattaagta tgaaaaaata tttcaatttt attttttcgt
     6841 ttcaaattat tctattagtg acatccttga actataaaaa taggagtacg gcatcatctg
     6901 ccgcactcct atttttttcg tcatttattt cttaaataaa acgattctca cgcaatttta
     6961 tttttttgat atcttctact gtttgtgttc cagctaattg catgaccatt tccaattctg
     7021 ttttaaagaa atcaaacact tgatggactc ctgtacttcc tcctaaagac aatccataaa
     7081 taacgggtct gccaatagca acaagatcgg cacctgatgc gattgctttg aaaacatgtt
     7141 gtccacgtcg tacaccgctg tcaaacacga ttggtgcacg tttatctact gcttctgcca
     7201 catattgcaa cgaatcaaac gcagcaggcc ccccatctaa ttgacggcca ccatggttag
     7261 atacccaaat tccaccagca ccagattcta atgatcgata gacatcttct tctgattgaa
     7321 cacctttcac gtagactggc aaatcagaat gagcagcgat aaattctaca tcttttggac
     7381 taagtttttg tttggaagat ttatatacag catccatcgt ttggcctact cctgattgat
     7441 aagcctgaac gatcggcatt ggcagcggga aggtaaaccc gtttctgcgg tctgtttcac
     7501 ggtttccgcc aactgttgca tccgctgtca atacgattgc ttttgctcca ttacgcttgg
     7561 ccacttcaag aatatccaaa ttgattccat tatctttgct catataaaat tgaaaccatt
     7621 gcggtgcttt ctccccgcct gcttctctta tctcttctaa agtacaagaa gcgtaagaac
     7681 ttgctgtata gatcgtccca aaatctgcta caccttttgc agaagctttc tccgccttca
     7741 catgagctaa tccatgagca gcaactggtg ccatgatgat tggtgcagtc agcatttctt
     7801 cgtcaaaatg tgtagttgta tcgggtaatt ctacatctct taatacatga ggaataatca
     7861 aacgatgatt gaatgcccgt tcattttctt gataagtaaa taagtcgcca gcaccactgc
     7921 tgatgtaacc ataaccacca gctggaatca cttgtgaagc cgcgatttct aaatcttcca
     7981 tattgataaa atctactatt ccttcatttg tacctgcttg atatgttttt tccatctcaa
     8041 tgccttctct ctataaaatt tgaatgatga acaatatgat agcgtttaca ctttttatat
     8101 tactcttcga cattgataca ggcaaataat ctgtttacat ttcagccatt ccagatgtca
     8161 ctactctgga agaaatgatt gtaataaaaa tagataaaaa accgatggga ctttgacaaa
     8221 agtagtgtca aagtcccttt ttttgaataa acggtgttca aaagccattt tctcggtatt
     8281 aagtcaaatt tatgcaaaaa tgtggaaagc cattttcaca aaatttttct tactactcga
     8341 aactaagcga ctttttcaca acctctcgat aaaacggtgt tcaatcagct gaccctcggt
     8401 attagctcaa aaaatggcaa aatatgagga actattttgc catttgtctc tctaatactc
     8461 aggtcagaac gttgacttca cagtctcttc cttaaacggt gttcaaaaac ttgcatctgc
     8521 ggtaataagc ccaaccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatt
     8581 acttgatgca gggcaggttt ttcacgacct ctgattcacg gtgtttcttt ttcatcaaca
     8641 tttttacgaa atactgtgga agactattga aataagtgtg gattgtttct ttgtgtttca
     8701 atcgtttctt gccattcttc atttaatttt ggattaccgt tttcttcctt gaactcataa
     8761 ggtcctaatt caaaaatccc agacagtatt tgattcacga tttcatatag ttcagtgtga
     8821 tcagacacat gattcttgaa gatcatctca ttatcaccac gtttgaacct cacattcggg
     8881 ctctcatgat gaacctctat gcatttttct ccttttttta actgacggac ttctgaaaac
     8941 atctcataaa tatcttctcc agtaaaacct tcagcaagcg ccagactgtc cacaagaccg
     9001 cctactgtca gttcagagat tattgcttga ttggaatcta attttaatgt atagcttgtc
     9061 gcttctacta cgccacatgc attgcaaccg ccttctgata gttcgctttt tctaatcaat
     9121 tgaacttctt tcattttctc ttcctcctct taattttcta cttatgtatt tttatcacct
     9181 gacataggtg actcctatca acgctatcat gtgctttttt cctcagaaat cctatttatc
     9241 cattccatct atcccttatt cttctacttt tctttctcta gcatataaga gatacgcata
     9301 gtattcaagc aaggattgtc gatataaaaa gaacaaatac tgctattttg atttcttttg
     9361 gctatattac atataaataa aatttatcat acctgctctt tttgactata tcatcatctg
     9421 acttctatac ttcgtttgta ttaaaactta ggaggaatca ttcatgctta cagggttaaa
     9481 cttattaaat aatccttttc taaataaagg aacagctttt acaaaagaag aacgggcaaa
     9541 atatggaatc actggtatgc ttccaagtac tgtccaaaca ctggaacaac aatctgttca
     9601 agcttatggt caatacttaa gcaaacaaac agatttggaa aaacgtatct tcttaatgaa
     9661 tctattcaat acgaatcgga cattgtttta taaattgatg ggcgaacatt tagttgaatt
     9721 catgcctgtc gtttatgatc ctgtcgttgc ggattcaatc gaacaataca acgaaatttt
     9781 cttgaaaccg caagatgctg catttctttc aatcgacgaa ccagaaaaca tcaaagcaag
     9841 tttgaagaac gcggcagatg gacgagacat tcgtttgatt gttgtgactg atgcagaagg
     9901 aatcttaggt atgggcgact ggggtgtgaa cggtgttgac atcgctatcg gcaaattgat
     9961 ggtctatact gctgctgctg gtattaatcc agcacaagtt ttgcctgtat cgatcgacgc
    10021 tggtactaat aatgaagaat tattgaataa tcccttatac ttaggcaatc gtcatgctcg
    10081 tgtagaaggc gaaacttact atgaattcat cgatcaattc gttcaatcag ctaccgaatt
    10141 attcccagaa ttgttattgc attgggaaga ttttggacga ggaaatgctg cagcgatctt
    10201 agaaaaatac gaagatcaaa tcactacttt caacgacgat atccaaggta caggaattgt
    10261 tgtcctagca ggtgtattag gcggattgaa tatatccggt gaacaattga aagatcaaat
    10321 aattttgaca tttggtgctg gtactgctgg tgtgggtatc gcaaacatcc ttttagatga
    10381 gatgatccgt caaggtgtac ctgaagaaga agcacgccaa catttctatc aagtagataa
    10441 acaaggttta ttgtttgaag atacagaagg attgactcct ggtcaaatcc cattcgctcg
    10501 caaacgctca gatttcacta atgctgatga attgacaaac ttagaagcag ttgtaaaagc
    10561 gatccatccg acaatcatga ttggtacttc tacccaacct ggagcattca ctgaagaaat
    10621 cgtgaaagaa atggctgctc atacaccacg tccgatcatc atgcctttgt ctaatccaac
    10681 aaaattggca gaagcaaaag caaaagattt aatagaatgg acggacggaa aagcattagt
    10741 tggaacaggt atccctgctg ctgatgttga atataatggt gtaacttacc aaatcggtca
    10801 agcaaataat gctttgatgt acccaggttt aggacttggt ttgattgctt caacagctac
    10861 acgagtaaat gctgaaatca tttctcaagc tagtcgagca ttaggcggaa tcgttgatgt
    10921 aacaaaacca ggagcagcta tcttgcctcc agtagcaaaa atcactgaat tttctcaaac
    10981 gattgctgaa actgtcgcaa aatcagttgt cgcacaaaac ttgaatcgtg aagaaatcac
    11041 ggatatcaaa gaagcagttg aatctgctaa atgggttcct gaatataaat ctttagaaga
    11101 ttaagatcgt tatagtaaaa gaacatacag aaactttgta tgacgtctaa aaagaaaaaa
    11161 gcgactgcgg caaaacagcg gccagccaac tcctgctctt ttgtcgcttt tctttggctt
    11221 ttttcatatg atacagaata agaaaggatc tgtaaaaatg gcacaaaaaa caacaactgc
    11281 aactgcagtt gaggaaaaga aaaaagaagg tttttggtcc acaaaaatca ccggcgtaag
    11341 tttgccactt tatttgatca tggtccttgt cctgatcatt gcaatggctt tagggaaatt
    11401 accgacgaat atgattggac caatcgcagt tttggttatt ttcggtaatt tatttcattt
    11461 tatcgggaca aaaatcccta tcgtcaaaag ttatttgggt ggtggttccg tttttgcgat
    11521 cttcgcttct gctgcgattg ctacatttgg gatcttacct gaatatgtag tgaaatccac
    11581 agaaaacttt gtcagcaata tgggcttttg ggacttttat attgctgcct tgattactgg
    11641 tagcatcctt ggtatgaacc gtaatctatt gatgaaagct tctgttcgtt ttattcccgt
    11701 tgcacttatt tcaatggtcg tctccttttt tgccgtagga ctcgtgggca tgcttatcgg
    11761 aaatggtttt gcaaattccg ttttatacat ctcattacct gcgatggctg gtggtgttgg
    11821 agccggagcc gttcctttat caacgattta tgcaaatgtt ttaggtacgg atgcttcttc
    11881 tatcatttcc cgcctgattc ctgcgacagc aatgacaaat gtcttagcta tcatcggtgc
    11941 agcattagtt gctcgtgctg gacaatcaat gccaaaattc aacggcaatg gtaaattaat
    12001 ggaaaaaggc gatcttggcg atggaaaacc tcgaactgtg aaaccttcca ttccacagtt
    12061 aggtgttggt ttgatggtat cattgacttt cttcatctta ggacaaattg gtaactattt
    12121 tgttcctaaa gtccatgctt acgcatttat gattattatc gttgtcatct gtaaaatcgc
    12181 caacctatta ccagagtatt atgaagatgc agctatcatg ttcaataact tgattgtcaa
    12241 aaacttgaca gcagctgttc ttgccggtat cggtattgcg ttgctgaatc tgaatgtatt
    12301 agcttctgct ttgacatggc aatttgttgt cttatgtctg acaagtgtta tcgcgatttc
    12361 agttgtttca ggatttgtcg gtcgtttgtt tggtttatat ccaattgaat caactattac
    12421 tgctggctta tgcaacaact cgatgggcgg aacaggaaat gtagccgtct tatctgcagc
    12481 taatcggatg gaacttattg cttttgctca aatgggcaat cgacttggtg gtgccatcgt
    12541 tttgatcatc tctggtttct tgatgcaatt actttcttag tcaaaaaaac aaaaatacct
    12601 gatcatcaag tatcggcttg gtgatcgggt atttttttga taaagattta caggattttc
    12661 aaaaatcttt tttgcatttt atcggaatga taaccgatgt gttcataaaa ttggtgtgct
    12721 tctactctgt attctgcaga attaagcctt attgtcgtta ttcctcgttt tttcgcttct
    12781 tcttctacgc cagtcatcaa agcctttccg atgcctcttt tttcagcata tttagacaca
    12841 gcaagtgcca taatattgaa cattggatcg ctaaaaatcg attcatagga ctccgcttgt
    12901 acatagccag ctgctttttt agaagtagga tcttcataaa ccaagaaaaa atttttctct
    12961 ggctccttca gcagtttctt catttgtctc accgtaacgg ttaatggtat atcataccct
    13021 aattgatccg cattgatttc acgtagtgct tctgcatctt ctaaacgcat ttcccgtaac
    13081 atccaaatca ccagccaatt tctgattttt taaaatgaat ttttttatca tagtatactc
    13141 tttttttcat ttagaacaat atgtttcagt gattttgact tgcgttagtc tagattctcc
    13201 taatgtttgt aagatgacaa aggcattgta acaattgacc aaaagaacca cgaggacaat
    13261 tacttgaaaa ataaaggtca ctttttttgg agacaaaacc ctgctaaatc ttgctcccca
    13321 caaacctcca ataacagcag cggggataac ataaaacaac atagataaat caaagcgcat
    13381 aaagctactt gttaaagcaa tcgttaccaa ttttgccagt tgagaaaaga aaatcgtaga
    13441 tagagaatat agtgttgctt cttttattgg caatgcaaac atcagcatca acaaagaaac
    13501 attgactggt ccaccaccaa tacctaaaaa actagctaaa aagcccagaa cccccccaca
    13561 tataaggtac caagtccatg acgtcaaatg aaattttggc tgatgatgct tggtgtagaa
    13621 gaacgcaaaa accaaggtca ataccgtcaa aaaaatttgg atcagctgca catgcttctc
    13681 gttttcaaat aactgtaaaa aaacttcgaa tacaatgttg ccgactactc ctccaacaac
    13741 agctccacta gatacccaac cgacaatctg ccaattcagt gatttcccgc ttgatacttg
    13801 tcgcacagtc gaaacaatgg acatcgtgaa taccgcaact gttgagtaaa aagaaatccc
    13861 tgcaacagaa tgggcgccga taagatcaag aatcggtttg atcaacacgc ctccacccat
    13921 cccggaaata gcgccaactg tgttcgccag aacgatcacc acaaaataaa tcagaccaat
    13981 catcttgtat ttcctttctc ttgtttatat cttttagcga ttgttactct tttcttttat
    14041 tatacagtaa aagaaacaaa cgaggcttcg aaagcttttc tactttttaa ggaaatttag
    14101 tgctaaacga aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa
    14161 caaaaatagc tcctcataat tttcgtttgg ttgaacttat taccgcagat gcaagctttt
    14221 gaacaccgtt taagaaagag gtcgtgagcc tgccgttcag ctccgagtca caaggaacaa
    14281 tttcaaaaaa cagcttctca tgtttgttga atattgtgac ttgtgaccga ggagttggca
    14341 ggagaacacc gtttaaggaa gagactgtga agtcaacgtt ttgacctgat tattagagaa
    14401 acaaatggca aaatagttct tcatatttcg ccgtttttcg agcgaatgcc gaggggcagc
    14461 tgattgaaca tcgtttattt ggaaaaaggg attgtgacaa gacgtttgtc acaatccctt
    14521 tttttatatc ttattaattc at
//
LOCUS       DACDSY010000071        14311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDSY010000071 DACDSY010000000
VERSION     DACDSY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.071"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IWK44_001743"
     CDS             <1..888
                     /locus_tag="IWK44_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP9550249.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            complement(928..1620)
                     /locus_tag="IWK44_001744"
     CDS             complement(928..1620)
                     /locus_tag="IWK44_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9550250.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            1705..1881
                     /locus_tag="IWK44_001745"
                     /pseudo
     CDS             1705..1881
                     /locus_tag="IWK44_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(2057..2626)
                     /locus_tag="IWK44_001746"
     CDS             complement(2057..2626)
                     /locus_tag="IWK44_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9550251.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(2700..3023)
                     /locus_tag="IWK44_001747"
     CDS             complement(2700..3023)
                     /locus_tag="IWK44_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550252.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(3177..3989)
                     /locus_tag="IWK44_001748"
     CDS             complement(3177..3989)
                     /locus_tag="IWK44_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550253.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            4343..5191
                     /locus_tag="IWK44_001749"
     CDS             4343..5191
                     /locus_tag="IWK44_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP9550254.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            5336..6277
                     /locus_tag="IWK44_001750"
     CDS             5336..6277
                     /locus_tag="IWK44_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP9550255.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            6267..8438
                     /locus_tag="IWK44_001751"
                     /pseudo
     CDS             6267..8438
                     /locus_tag="IWK44_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(8550..10721)
                     /gene="copB"
                     /locus_tag="IWK44_001752"
     CDS             complement(8550..10721)
                     /gene="copB"
                     /locus_tag="IWK44_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP9550256.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(10741..12927)
                     /locus_tag="IWK44_001753"
     CDS             complement(10741..12927)
                     /locus_tag="IWK44_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP9550257.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12927..13136)
                     /gene="copZ"
                     /locus_tag="IWK44_001754"
     CDS             complement(12927..13136)
                     /gene="copZ"
                     /locus_tag="IWK44_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP9550258.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(13149..13589)
                     /locus_tag="IWK44_001755"
     CDS             complement(13149..13589)
                     /locus_tag="IWK44_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP9550259.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(13663..14202)
                     /locus_tag="IWK44_001756"
     CDS             complement(13663..14202)
                     /locus_tag="IWK44_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9550260.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         4156 a         2927 c         2530 g         4698 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt
      961 gtcatgaaaa tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat
     1021 catgacgtat actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac
     1081 atttcctaaa gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac
     1141 tctagctagt tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt
     1201 agcaatgtgc catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat
     1261 gtgggcgtaa tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc
     1321 aattactggt ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc
     1381 ataggctagt ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg
     1441 atcgccccaa atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg
     1501 agtgtctaat gttaatggaa taccatattt cattgctgaa tctcttgcca attcaaagta
     1561 tactttatag ttcttttcaa aagtttcttt atcatgtgta tgttgtaact cgatttgcac
     1621 aggacttttg gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg
     1681 aaaacaccaa cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta
     1741 gaagatatca aaggtttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat
     1801 atttctcccg aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa
     1861 ataaaaaagc actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa
     1921 ttttatttat aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt
     1981 atcttaaagg cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag
     2041 tgcgaacatg gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca
     2101 tcaattgaga ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg
     2161 gttttcttct taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca
     2221 cgactccgcc aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta
     2281 gattttttgc tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct
     2341 cttccatccc ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta
     2401 tttcttttgg ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca
     2461 agagcgtttc gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata
     2521 tatatacgcc agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca
     2581 cttctgtgat cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt
     2641 tactaatcgc tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt
     2701 tatcggtttt tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa
     2761 ggatggtaca gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcgatg
     2821 atcactagat ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt
     2881 tttcgatgtc ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca
     2941 tagcgaaaaa aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc
     3001 tggagtgttt tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg
     3061 atcgctagcc gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc
     3121 tgataataac gaacttcttc tatcagccgg tcatggatac caattacgta acgaagtcat
     3181 tcttggtaaa gtacttttgt cagctttgat ttgctttcgc tctcaaattc gctattcagc
     3241 caaaacagcg aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct
     3301 tctatccgtt tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga
     3361 taattttttt cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat
     3421 tcaaagaata gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc
     3481 ttcaataatt ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc
     3541 ggaaaatctt tcaaaaagca aaacagcaga tatcttactt ttctttctga accaaagatc
     3601 tgtgcttttt tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct
     3661 tgcgtagaac gatcgtactt ctgctcgtaa ggttcattca atagcagatt tttcagtaaa
     3721 caaaacgggg gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga
     3781 tcataaacta actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc
     3841 gccaaacgtt tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa
     3901 tcagcttttc gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca
     3961 ttttttgata atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat
     4021 tgtttgatca aaatgggttc actcctaaaa agataaaaaa ctccctttct atttatttaa
     4081 atacgcaaat cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa
     4141 aacgaatgta ctttaaaaaa acataataag atcactttgc ttgtatgatg tattcccttc
     4201 cattacaatg aaataaatag cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat
     4261 agaagaatcc aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcag
     4321 caaacgttca actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt
     4381 cgaagatatt cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca
     4441 gggaaccttc agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt
     4501 ttccgtatac aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac
     4561 gatcttgtta gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga
     4621 agcaatggcc gagaatcatg caacgatcga caacaataca taccctctag acaatcattt
     4681 ttttgtctta gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc
     4741 acagctcgat cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact
     4801 tttgctactt ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag
     4861 ttatgaaata gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc
     4921 atcttatgcc ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat
     4981 cagtccacga ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg
     5041 gagaaattac gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg
     5101 cctgattttt tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct
     5161 tgttgcacaa attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag
     5221 attatttcat taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg
     5281 tattcaacaa ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga
     5341 gcttgatttc attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac
     5401 tcgtttatcg caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt
     5461 ctttacctct ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga
     5521 gattttgatt cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag
     5581 aggatgccat gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag
     5641 aaaaagtgca aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat
     5701 tcaagcagaa aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag
     5761 aggtcttgat tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga
     5821 ttggaaattg tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga
     5881 agaatgttct cctctttttt gcttttgggg tttgcccagt ccaaaatttg aacctctgct
     5941 agacttgtat ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt
     6001 aggcaaccgc gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc
     6061 tggcgatgag aaagcttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct
     6121 ctttgttcct gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg
     6181 aacggtttat cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat
     6241 atgttcattg ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg
     6301 tttactcctt tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc
     6361 aaaacagcta ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc
     6421 ctaaaaaatg ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat
     6481 attttccttc gaagacattt tccatattat ggatcttaca gtttttttcg tcagtcacag
     6541 aggaattctt ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt
     6601 ttctcataat gttgctgatc tatttattta ttacattgac cgtggattac gactattggt
     6661 acgcaccctt tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag
     6721 atttactgta cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc
     6781 aatttttctc catccggttg ttgtcgggaa acactcgatt ccagtcattg actacagttc
     6841 tgcttatttg tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacacc
     6901 aatctctcga ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg
     6961 acacactcca tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg
     7021 aacagcttgg tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaaaaag
     7081 gaaatggcta ttggaagatc gaaaataaaa atcattacac cggttctgga tggatagaag
     7141 atcaggacgc acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa
     7201 ccgttataga agacaaagaa aacattacat tgaccctaaa aaaacaagtt ttcttacctt
     7261 atccttatgg aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact
     7321 atttgcctga aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt
     7381 tacaaacaga aaggttagtt tttacgagag aagaattaaa aagcagtcag ttaaaagctg
     7441 atgatttaga aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg
     7501 aggacttggc ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg
     7561 tctaacagta tttaaaaaca agcggaaatt tccgctattc caaaacggat accgggcata
     7621 ctccaaaaga acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg
     7681 acaacttttc aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga
     7741 caaaaggttt tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca
     7801 gcattctcaa tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc
     7861 ttccatttga accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa
     7921 caaccgaaac cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa
     7981 ctatttccag tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt
     8041 ataaaatcgt cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt
     8101 tattcagaaa atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta
     8161 ctaatacgat tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac
     8221 ccgcagaatc tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc
     8281 gtttcattcg attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg
     8341 taaaattcga taaaaaaacc tatttagaaa taacgcatat aataagaaaa atggtataca
     8401 aaaaaattct gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta
     8461 cttctggaat gccgagaacc agctgattga gtatcgatta ctcgaaaaaa aggttgtgac
     8521 acgttttttg tcacaacctt ttttcattat tatctgattt tcaatgtcaa agcatttaga
     8581 gctacaacta ccgtacttaa cgacatcaag acagcaccaa ctgctggact taatatgata
     8641 ccaattggcg caagaattcc ggctgctagc ggaattgcaa gaatattgta acctgccccc
     8701 caccagaggt tctggatcat tttctttcgc gtttgcttgg ctagatcaag aaaatgcaaa
     8761 atatcttttg gatcgctgtt tgtcaatacg acatccgcag aatcgattgc gatatctgtc
     8821 cctgcaccga ttgccatccc gattgaagca cgggctaaac ttggcgcatc attgatccca
     8881 tcacccacca tgattacttt tttcccttgg tcaaggtatt tctggacaac agcttcttta
     8941 tcatctggta ataaaccgcc ataatattct tcgatcccta aataatctgc tactgcttgg
     9001 gcagcgtttt tattgtcacc agtaagcatg acaggagtga tttttctttc tttcaatgtt
     9061 cggataaatt cttttgcttc tgtttttact acgtcaccta aagcaacgat tgcagctaga
     9121 tgattttcta aaatcaggaa gctgatggta tttccttgtt cttggtaagg tttcaataat
     9181 gcttgttcta cgtttaatcc gagacgctca acttcttttt cattaactat tttcacttgt
     9241 tgttttttga ctgttgcctc tagcccgact cctgaaagat tttttagatt ttcagcttga
     9301 taaggcttga tttcatgttc tttcaaataa ttcataatgc caacagccaa tggatgattg
     9361 gcattcgctt ccaaggctcc tagatattga aggatttcat ttttattgaa cgcatcatca
     9421 agtacttcga cacccgttac agtaaatttt ccttctgtta gtgttcctgt tttgtccagc
     9481 atgatcacat ctagatcatt ggcttgctcc agtgcattgc ggtttttcaa aagtaatccg
     9541 tttttagctg ctattgatgt ggaacgagca acaactaatg ggatagctaa acctagagca
     9601 tgaggacagg cgatgataaa gactgttacc atacgttcta atgcattagg aagatctgtc
     9661 aagaacaacc aggcgatgaa tgccaggata cccacaatta atgcgacata aaacagccat
     9721 tttgccactt tgtcagataa tgactctagc ttggattttt ctccttgtgc ttttcgcacc
     9781 atttccataa ccttcgctag ataaccattt tcaccagttc cagttacttc gatttcgatc
     9841 gttccatccc cattgataga accgccaatc actttgtcat tggcttgttt tttgattcct
     9901 cttgattcgc cagtcacagc tgattcgtca acaattgttt cacctttcaa gattacacca
     9961 tctgttggca ttttatcacc agaccggacg atcaaacgat caccttcgtt tacttctttc
    10021 aatgaaactg tctcttcttt tccatctttc gtcaagcgtt tcactgattc tggcagtaat
    10081 tctgctagtt tttgtaaagc attgctggca ttcgacacag cattcatttc aatccagtga
    10141 cctagtagca taataacgat caaagtagcc aattcccaga agaaatccat gacgtgtgta
    10201 tgtggattga gcagatttgc gataaacgaa tagacactat aaatatatgc aactgtaatc
    10261 cccatcgcga tcagtgtcat catagcagga cttttttgtt tcaattccat cttagctcca
    10321 cttaaaaatg gctgaccgcc atagataaat agtatagttg ctaagatcaa tacgagccaa
    10381 tcagaaccag gaaacgttac ttgaaatgga aattccattc ccatcattgg tgaaaaaaga
    10441 atgattggga tagctaggat aagcgatagc caaaattttt gtttgaaatt ccccatgtgc
    10501 atggaatgat ccatgccgcc gtgttcgtga tccatgtgca tcgaatggtc catttgtttg
    10561 ctgtttgcta tatctccatg gttatgtccc atgtgcatgg aatgatccat ctgactatgg
    10621 tcatgtttca tctccatctg tttttcatga gattctgtac tttctttgtt tactacttct
    10681 tttttatgct catgtttttt ctccatatcg ttcccattca tttttactcc atcccttctc
    10741 ttactttttc atgtgtcgat ttaagcacag ggagttcaac aatacactga cggaactaaa
    10801 cgccatcgct cctcctgcaa tgattggatt caaaaagcct agtgcggcaa aaggaatacc
    10861 aatcgtatta tagacaaaag cccagaaaag attttgtttg atttttctca atgtcaacct
    10921 agacaactcg attgtttgtg caatggatgc caaactgcta ttcatcagcg tcacatcggc
    10981 tgtttccatc gcaatgtccg ttccacttcc cattgccata ccgacatcag ctaatgctaa
    11041 ggccggtgca tcattgattc catcgcctgc cataccgact tttttgccat ctttctgaag
    11101 tttttctacg taggctgctt tatcctctgg aagtacttca gcaaagatat gctttggatc
    11161 aataccaact tgtttaccaa tcgtttcggc tgccagtttg ttgtctcctg tcagcataaa
    11221 aacatctaat cctttattct ggagctgctt gattgcttgt ttgacttcta gtttgatttg
    11281 atcagccacg gcaatcaacc cgatcacttg cttttcatca gctaaaaaca tgactgtttt
    11341 tccctgctgt tctaattcta acgcgtgttc ttggtattca tcaaatgata ggttcaattc
    11401 aattaatcgt ttacgtgtac ccgcaaaata gcgtactccg ttaatcgtac cgctgattcc
    11461 agcacctggg tgggctgtaa aatcaatcat atcgtatgtt gctgcttctt gcagttttcc
    11521 gtattccacg attgctttac ctagtggatg ttcagaagct tgttctaaag tataaaaaat
    11581 agataggact tctttagaac cgactagatc tgtaactttg ggctttcctt ctgtaatcgt
    11641 tccagtttta tccaaaacaa tgctgtctaa gtgagcagct gcttctaatg cttctccgcc
    11701 tttaattaaa attccatttc gtgcaccaac acccgttcct accataatag ctgttggagt
    11761 cgctaaacct aatgcacatg gacacgcaat gactaataca gagacactat ggatcaatgc
    11821 gagttgccaa tcaccagtga tcaaacctgt agctatgagc gtgataaacg cgatgactaa
    11881 tacgatcgga acaaaaatcc ctgagatccg atctgcgatt ttttggattg gggctttgct
    11941 cccttgagca tcttccacca tttgaatgat ttgcgctaat actgtctgct tgccgacttg
    12001 tgtaacttcc atgtgaagta gaccattcgt attgattgtc ccgccaaaga gcgtgtcatc
    12061 cggattcttt tctacaggaa gattttcacc agtaagcata ctttcatcta tcgcactgct
    12121 tcctgaaata attcgaccat ctgctggaat ctgctcacca ggacggatga ctagttgatc
    12181 accaacgact acttcttcaa tcgctaacgt ttcttctttc ccgtttctta tcacttgggc
    12241 ggtttttgtt tgaagagaca tcaattgttt gattgcatta cctgttttcg tttttgctgt
    12301 atgttccaaa tatttgccga gcaaaatcag cgtgatgatc atactgctgc tttcaaaata
    12361 aagatcttgg gggtgtccac ggaaaaatcc gttgtaaatg ctcaatgcaa aggcagccga
    12421 tgttccaatt gctaccaata catccatatt tggtgctttc gtcttgattg catggtacgc
    12481 acctttataa aaacgagcac cgacataaaa ttggacagga gcagacagca ctaactggac
    12541 gattggaaaa tgaaaaaagt gaacaatcgc cgcatggctt cctaacatca tagcaatcat
    12601 tgacaacatc aacggcaaag tcaaaaccgt actcaatata agatcgaata gcattttttt
    12661 caaatacgct tgcttttctt ctgcaatttt ttgtttgtgg gcttcatcgt acaaaattgc
    12721 accatagccg atgttttcta cgctttggat taatttttca gtggtcgtcc cttcatattt
    12781 gacacttgct ttttcagtgg ccaagttgac tgtggcattg accacaccag gttgtccgtt
    12841 cagctctttt tcgatcctag cagaacaatt tgcacaagtc atacctgtaa tgacgaacgt
    12901 atccattttg gtttctgctg ttgccatcaa atcacctctg ctttataacc caatccattg
    12961 attgcttgac aaatcttttc ggattgaact tttgtttcat caaacttgac cgtaccgttt
    13021 gcttttttca aatttacttt cactttttgc acgccgtcaa gtgcgcttac tgtttcttct
    13081 actcttgctg cacaatgatt gcagctcata ccttcgattg aaaattgttg tttcattttt
    13141 tcttcctcct attgatgttg tttacattca cattgacctg ggatacagtt acattcgatc
    13201 gtatctactg gttgcttttg ttccagttgt ttcaaaatct cattgatatc ttcttgagtc
    13261 aaagtggctt cttctactag atccgcaatc gtctctccaa tcctttttgc acagatatgg
    13321 gaaaaaagat tttccgtcgc actgcgaact gtttccatct ccgagacagc tggataataa
    13381 atgaattttt ttccttcttg ttctgtccag atcacttctt tttttaccaa acgacccagc
    13441 aaagttttga tcgttgctgg tttccaatcc atcgaatcag ctaagatctg cgtgatttct
    13501 tgggctgtcg tctgtccaag tgtccaaata acacgcatga tctcccactc tgaatcactg
    13561 atctttactg gcgttttgac tttttccatt tatttcacac ctttcgttta caaatgtaat
    13621 cattatctta tgaatacaga ttacacttgt aaacaaaaac tgtcaattat ttttgctctc
    13681 tattttcaat ttagttaaaa attcttgttt ctctcgtctt gttttaagga tgatcagttt
    13741 atttcttgca ttagctactt ggatcagctg ctgtattttc ggttcattgt tttcttcaaa
    13801 atgccagata aaagataaaa attctaagta ttccctgttt agtttctccg aaaatgattc
    13861 aggcatatct ggccgagatt ttttgtttat ctttcttttt agagaacgcc aaactactcg
    13921 gtagatggcg agataacgcg gcactttaag ccaaatgatc ttatctgctt ctttaatcct
    13981 ttcctctaat gtagaagtat aatttccttc aacgatccat gacggattag ataccataaa
    14041 caattgctgt tcttgcaaaa accacttttt tgcctgaata ctgtagtctg ttgtatgcca
    14101 taaaaggtct agcggcagta gcggaaagtc ggtgatttct ttgatcttcc ttgcgagggt
    14161 cgattttccc gacccacttt gtccaatcac gatgatcttc attcttctcc ctcacttctc
    14221 tttggaattt ggttcgatca taccacttat aacgccgcaa aaaaacaagg gactgtgaca
    14281 aaagtcgtgt caaagtccct tgtttcgaat a
//
LOCUS       DACDSY010000072        14097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDSY010000072 DACDSY010000000
VERSION     DACDSY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.073"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            56..757
                     /locus_tag="IWK44_001757"
     CDS             56..757
                     /locus_tag="IWK44_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP9550261.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            754..1875
                     /locus_tag="IWK44_001758"
     CDS             754..1875
                     /locus_tag="IWK44_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP9550262.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1890..3122
                     /gene="pepT"
                     /locus_tag="IWK44_001759"
     CDS             1890..3122
                     /gene="pepT"
                     /locus_tag="IWK44_001759"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP9550263.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3254..4162
                     /locus_tag="IWK44_001760"
     CDS             3254..4162
                     /locus_tag="IWK44_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP9550264.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4197..4469
                     /locus_tag="IWK44_001761"
     CDS             4197..4469
                     /locus_tag="IWK44_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP9550265.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4480..5526
                     /locus_tag="IWK44_001762"
     CDS             4480..5526
                     /locus_tag="IWK44_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP9550266.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5777..8386
                     /gene="clpB"
                     /locus_tag="IWK44_001763"
     CDS             5777..8386
                     /gene="clpB"
                     /locus_tag="IWK44_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP9550267.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8537..9208)
                     /locus_tag="IWK44_001764"
     CDS             complement(8537..9208)
                     /locus_tag="IWK44_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550268.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9201..9695)
                     /locus_tag="IWK44_001765"
     CDS             complement(9201..9695)
                     /locus_tag="IWK44_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9550269.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9715..10935)
                     /locus_tag="IWK44_001766"
     CDS             complement(9715..10935)
                     /locus_tag="IWK44_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9550270.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11082..12917
                     /gene="lepA"
                     /locus_tag="IWK44_001767"
     CDS             11082..12917
                     /gene="lepA"
                     /locus_tag="IWK44_001767"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP9550271.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13011..14093)
                     /locus_tag="IWK44_001768"
     CDS             complement(13011..14093)
                     /locus_tag="IWK44_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9550272.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4554 a         2591 c         2919 g         4033 t
ORIGIN      
        1 ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg aagctatgaa
       61 ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg aatccgctcg
      121 tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat taagaggaaa
      181 aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag cacgtaagca
      241 agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg gtctagatgc
      301 tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag gttcactgat
      361 ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag aacgtttgat
      421 ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa atcagtataa
      481 aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca ttgtagccga
      541 aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc catttctgct
      601 tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac aaagaaaagc
      661 tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac aaatcgagca
      721 ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg cactttcatc
      781 caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc agtcggactg
      841 catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga cgttcgtcca
      901 gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa acatcctcca
      961 atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat gtatgcagat
     1021 ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat tatttgggat
     1081 ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta tttagtaaag
     1141 actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca tgcacagaaa
     1201 atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac cggtacaagt
     1261 tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc agcgattggg
     1321 aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt gccggaaact
     1381 atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga acctgcttat
     1441 gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt aggtgaattg
     1501 ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt ccaactagat
     1561 ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat tgcgatttgc
     1621 ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga tgtctatatc
     1681 acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt gattgcaatt
     1741 gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa atttgaatct
     1801 tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa tacaaaccca
     1861 tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca cgttttttac
     1921 gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca tcgactcaga
     1981 cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc ttagctgatg
     2041 ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat gttgatcatg
     2101 aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat gcagtaggag
     2161 tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta gatgcggaag
     2221 aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa ggccacacat
     2281 tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg attgcggaaa
     2341 tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt accatcaaag
     2401 tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat gttgagcaat
     2461 tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg cagtatgaaa
     2521 cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat ccagggacgg
     2581 caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat cagcttccag
     2641 ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg atggcattgg
     2701 atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat cgtgaaaaat
     2761 tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag cgatttgatc
     2821 aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa atcattgaaa
     2881 aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt atctctccag
     2941 tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg ggtattccga
     3001 cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt gtttctttac
     3061 aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac acaaagagtt
     3121 aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa tgtcatctgt
     3181 ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt gaagagtaaa
     3241 taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag ggaaagatga
     3301 gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc cagaattata
     3361 tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca agaaagcaga
     3421 agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc atcatatcgt
     3481 ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag gtggaactgt
     3541 caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg ctgacgtgat
     3601 cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag tcattatctc
     3661 tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag aagaaaaaca
     3721 gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga agaagaaaaa
     3781 atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc aactgttgac
     3841 agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag cgaccctaga
     3901 tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat ttatttttta
     3961 tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt atttcacagc
     4021 caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg atggagacac
     4081 gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag tttattcacc
     4141 aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga agagtaatgg
     4201 atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt tacttatttt
     4261 gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt cctgagaata
     4321 atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct cgaaaagttt
     4381 tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt gtacttgttg
     4441 atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa aggaacgaaa
     4501 actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat ttattattta
     4561 atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt tgttgttttg
     4621 gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc aatcaaagaa
     4681 attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac ctctagctat
     4741 attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga gatttatttt
     4801 tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact ccatatgatt
     4861 atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca attctctctt
     4921 ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct tttgacgatc
     4981 gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat attttttgtc
     5041 agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga tacatgcaat
     5101 cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag gactgcgcta
     5161 ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact tccttatttg
     5221 attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt tatcagcttt
     5281 gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt tgaatttgtc
     5341 tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc gcttttactg
     5401 tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt gattgctgaa
     5461 cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa agaaaatcaa
     5521 gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg ttatggataa
     5581 gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt tgttttttta
     5641 agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg accaaaattg
     5701 accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt acctttaatt
     5761 aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag aagcgattgc
     5821 tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg cccatgtatg
     5881 gaaaatcttt ttacaaccta atcattttgg tcgaaatttc tatacggatg ccggattgga
     5941 tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag ttgtttctgg
     6001 tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat tgaatgaagc
     6061 agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg ttattttagc
     6121 gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg gattatctga
     6181 aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga cctcacaaaa
     6241 ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac agcaagtcaa
     6301 aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg tcatccgtat
     6361 tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg tagggaaaac
     6421 ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg aaaatttaaa
     6481 agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa aattccgtgg
     6541 tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg atggacgtat
     6601 cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag aaggaagtat
     6661 ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt tgatcggtgc
     6721 cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg aacgtcgctt
     6781 tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc tgcgtggatt
     6841 aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat tagtagcagc
     6901 agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag caatcgattt
     6961 gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa cggaattaga
     7021 tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga aaaaagaatc
     7081 agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg aacttcgtga
     7141 agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca attctgtttc
     7201 agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag aaaataacta
     7261 tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag aaaaagaatt
     7321 gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag agtccgttac
     7381 cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa cgaaacttgt
     7441 agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac gagtgattgg
     7501 tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg ctggattgca
     7561 agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg tcggaaaaac
     7621 cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata tggtacggat
     7681 cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag ctcctccagg
     7741 ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa atccttatac
     7801 aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata tcttgttaca
     7861 agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt tcaaaaatac
     7921 ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg tgactgctga
     7981 tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg gaaacttcaa
     8041 accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga gcctagacaa
     8101 cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg aacatcaaga
     8161 aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg cttatgaacc
     8221 agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa caccactagc
     8281 taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta ctttacttga
     8341 tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt ccattgagac
     8401 tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt caaataaaca
     8461 gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct gcccgcttct
     8521 gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt tcaaagtaac
     8581 gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca cctatcgtat
     8641 ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac agtgtgccat
     8701 cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag aattcccaga
     8761 actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg aacaaccaag
     8821 gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca tagccaagag
     8881 ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag atgatactga
     8941 tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag taaaacaaaa
     9001 cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc atgaaaatca
     9061 atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc gaatattttt
     9121 gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca agtaaagcta
     9181 tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa tctttttact
     9241 tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat caaacctatt
     9301 gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc agcgattttt
     9361 ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac tgggatgcct
     9421 ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag cccatctagt
     9481 aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc aatcattcga
     9541 ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag atagatcgaa
     9601 agctcagaag agagctctct gatttcttta actaactgtt ggttatataa caaatcactt
     9661 ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt tttatcaaaa
     9721 ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact tcagtgtttc
     9781 ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat aattcatcat
     9841 caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt catttaagac
     9901 acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc aaggagcgac
     9961 aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa accgttgaag
    10021 gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct ctttgatctg
    10081 actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat gtacccagta
    10141 gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt cttgatcaag
    10201 atagaacatt tcttttacgt gaaatatttc ttcagctacc caatagaaca gaacacctct
    10261 tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat tttcgaaacg
    10321 ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt gttgggcaaa
    10381 tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa ccataccact
    10441 tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt tatcagacat
    10501 gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat aagagatcca
    10561 ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca tcaatccatg
    10621 gttagcatat tctggataag ttccaactcc agtcacacca cccattttat aacgtgcacc
    10681 gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat tttcgtggaa
    10741 ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa aagctcgttt
    10801 attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat aggaaagcaa
    10861 ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat tttctctaaa
    10921 atctttttga tccatttttt catctccatt tattagcttg ataacagtat agcaaaattc
    10981 tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg cttttcgttg
    11041 gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata aagaaaatga
    11101 aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac catgggaaat
    11161 ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc gaaatgcaag
    11221 atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt aaattaaatg
    11281 ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac ttgattgaca
    11341 ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc tgtgagggtg
    11401 ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc aatgtttatt
    11461 tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac ctacccgcag
    11521 cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat gctagtgaag
    11581 ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag cagatcgtgg
    11641 aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg atctttgact
    11701 ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac ggtatggtca
    11761 aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt acagaggtcg
    11821 gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat gtcggttata
    11881 tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta acgttagcag
    11941 acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg gtttattgtg
    12001 gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta gaaaaactgc
    12061 aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta ggttttgggt
    12121 tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc ttggaacggg
    12181 aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt aataaaacgg
    12241 atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta acgattcagg
    12301 atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt gtcggtgcag
    12361 taatggaact ttctcaacga aaacggggag aattcattac gatggattat ttagatgatt
    12421 accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac tttttcgaca
    12481 aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct ggttatcaaa
    12541 aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat gcattaagct
    12601 ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa aaactgaaaa
    12661 aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga caaaaaatcg
    12721 ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt tatggtggag
    12781 atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag cggatgaaac
    12841 aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa atggacgagg
    12901 acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa accatgttta
    12961 catggatttg ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa tcaagaattc
    13021 tttctaagat tttccatgat atcgaccgtt tcatttctca tcttatctgt tacatgcgag
    13081 taagtatcca tcgtaattga aattctgctg tggcccaatc gttcagagat ttctttcatt
    13141 tttgcgccat tttcgagaag aagtgtagca tgagtatgtc tgagagaatg gaagttaaaa
    13201 gagagggaga gtgcattcga tatcctccta gtattccatt tcactacact tggcgtaact
    13261 agctcaccgt cttccttcgt acatactgca tttgaatcaa tgtagagctt tccatacttc
    13321 attcgatttt ctaattgttg tttcttatgt tttttcagaa ttgctagcaa ggtttgtcca
    13381 ataaaaatcg ttcgattgga actgcttgtc tttggcgtac catataccca tgcgccgtca
    13441 ttctttacca tttgtttctc tacagtaatt gttccatttg aaaaatcgac atcatcccac
    13501 gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa atccaatatt caaaggaata
    13561 tagaaaggat gtccttcagg agtaatttct agcatatgat caaagtcctc aagagaaatg
    13621 atttttagat cttttttagt cgttggtcgt gcttcatatt ttggtatttt tacatacagc
    13681 ataggatttt gcttgattaa tccccaagga taaaccgcca tattcagcgc attcttaagg
    13741 acagagtgag taatagtcat tgttttcttc gagtaaccct ttttaaattc agcattgatg
    13801 aaattttgta aaagagcagg ggagagatcc gtaagttttt tctttcctaa ataaccgttt
    13861 atatgatttt tgatggtaaa tcggtagttt tcataggtat tgtattttag atttagttta
    13921 acgtattcct ccatccaaaa atcaaggtat tgttttactc gagtatccgt acctaaaaag
    13981 tattgtcctg tttcgtcaat atctgataaa acttttcgta aagcagcttc ggcctctggt
    14041 cgggtgtctc cgccaacttt ctccactttt tttcttgagc catcatcatt gatatct
//
LOCUS       DACDSY010000073        13844 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDSY010000073 DACDSY010000000
VERSION     DACDSY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.074"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(204..707)
                     /locus_tag="IWK44_001769"
     CDS             complement(204..707)
                     /locus_tag="IWK44_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9550273.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1066..2091
                     /locus_tag="IWK44_001770"
     CDS             1066..2091
                     /locus_tag="IWK44_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9550274.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2414..3292
                     /locus_tag="IWK44_001771"
     CDS             2414..3292
                     /locus_tag="IWK44_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550275.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3768..3884
                     /locus_tag="IWK44_001772"
     CDS             3768..3884
                     /locus_tag="IWK44_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9550276.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            4812..5150
                     /locus_tag="IWK44_001773"
     CDS             4812..5150
                     /locus_tag="IWK44_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550277.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5244..5474
                     /locus_tag="IWK44_001774"
     CDS             5244..5474
                     /locus_tag="IWK44_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550278.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            5874..6245
                     /locus_tag="IWK44_001775"
     CDS             5874..6245
                     /locus_tag="IWK44_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9550279.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6519..7211)
                     /locus_tag="IWK44_001776"
     CDS             complement(6519..7211)
                     /locus_tag="IWK44_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9550280.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7214..7999)
                     /locus_tag="IWK44_001777"
     CDS             complement(7214..7999)
                     /locus_tag="IWK44_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9550281.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8236..9636
                     /gene="lacG"
                     /locus_tag="IWK44_001778"
     CDS             8236..9636
                     /gene="lacG"
                     /locus_tag="IWK44_001778"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9550282.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9661..9978
                     /locus_tag="IWK44_001779"
     CDS             9661..9978
                     /locus_tag="IWK44_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9550283.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            9991..11664
                     /locus_tag="IWK44_001780"
     CDS             9991..11664
                     /locus_tag="IWK44_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9550284.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11678..13309
                     /locus_tag="IWK44_001781"
     CDS             11678..13309
                     /locus_tag="IWK44_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP9550285.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
BASE COUNT         4362 a         2260 c         2844 g         4378 t
ORIGIN      
        1 tttaagcctt taagctaaaa aaaaaccctt tttgttaaat aaggggaata tagcctgtca
       61 gtttcaaact gcaagtcgtc ctgctgcaat aatatagtag attagagatg aaaatagatt
      121 tttttaaacc aatgactgaa taaaaagacc gtcatagata cacattggaa tgtgtctatt
      181 acggtctttt tttacgaacg attttattga agacggacag ttcgggtcaa aaataaaaaa
      241 gctacgatca gaaataagaa taaaatgtat cccaaaataa tggctagacg gtctaactta
      301 tttgtcgata gatgtttgtt tagaagttga taggcccata aacctatcag tcctcctgtt
      361 gtattagtta tcaaatcagt gatgtccgct acgccaatac cgaataggaa ttgaatcaat
      421 tctgccaaga agcttacaag gaaaataatc cctaattttt tagaaaaaga aagtctcttg
      481 aaattgacat tcaaaagcac cccaaaagga aaaaagacaa tcaagttata agtggtctca
      541 cgtaataccg tcgtattttc aaaagaaaaa gtagaaaaag ggacgagatt gacattgctg
      601 taagaataag tgagaaattg tggaagatta aatgatagct tgaataaaat caaccataat
      661 agaaagaata aataaatccc taataaaaat tttgatacat accgcataga catctcctcc
      721 atttaaaaag tataccatcg aaaaccgctg aaaaatcata aaaaagtatg aagtttaagg
      781 accaacgtag ttatcgtacg aaaattagaa gctttttttt gttgggaatc cggtttcttt
      841 aacaaagcta agatagcgag ttgcttggaa aagttgctat cttttttctg tcaaaacgac
      901 tcggaatgtt cgttttttgt tctaatttct ccaagttgtt tactttttga tactaagttt
      961 atgaaaaatc aagaaatagt tgttatacta ggtaatgctg acaaacggga ttggcatttc
     1021 atcttggttc agcttttttt ccaattacta ggaaggaggg atagattgtt caagacttat
     1081 tttcgtttca ataaaataca agataatcct tttcgtatca tgaatctgat gctacttatc
     1141 tttattgtat tatggttagg ttacataaat catgattttt tgatttcaag tttttcatca
     1201 ttagggatct ttactttttt ctattatcaa aacataccga tgaaatattt gctgaaacgt
     1261 atgtttctga tcggttgcgg actgttggtt gctttcgttt tggggatctt atcgacttat
     1321 gttttatggc ttgaaccatt tgccgtagct ttggttgctt tttccagcag attcgtgttg
     1381 cgattattcc atatttctaa accaggtggc ttgttttttg cgatgctttc tgccatggga
     1441 acgagcatgc agctgccaat cgctcaatta ccgatcgtct cactttattt ttttatgggt
     1501 gtcgtttttg ccctgattgc ggcagtgatt acaaaattac tggattcacg gcctgagcag
     1561 acaattgaaa aagcaacttt aaaagaaaga tttcacgaag agcctttagt gatcattgat
     1621 tctgttttct acagcgctgc cttatttctt tccgtttatg tcagtcacgg cttgaatctg
     1681 cataatcctt attggttgac cttgtcatgt gcatcgattt tgctagcaga aaacttggat
     1741 gcgatgaagc atcgacaagt ccaatatctg attggatcga tgggtgggtt atgtgtgtcg
     1801 gcattcttgt catttgtgcc gtttacgcag ttgcagacca tttttctgat cacttttcta
     1861 tatggaatcg cacaatttct agttgctaga aattatgcag ttgctaatat ttttcttaat
     1921 ccgatggcgt tgatgctatc tacacttatt cgtggagcat acttgatttc tttgatcgaa
     1981 tatcgttttt tagggatcgt cataggaagt ttcatcggtt taggtgttgc gtgggtaatg
     2041 acggttggtt tgcaacatta tttaacggtt gttcgtcaaa aattagaata gaaggacaaa
     2101 tatatgtgcc aaaagaggtt atgacaagat ttttgtcaca acctctaatt caaggtgttc
     2161 aaaagccaga ttctcagcat taagtcaaat tcacacaaaa atgtggaaag gaactttcaa
     2221 aaaaatttta tatttattct atcaaaaaat tttgtttatg atggtaactg aataaacttt
     2281 cctgagatgg tcaatgcttt ccttgaaagt cttacagata ttctttcttc atagaaagtt
     2341 taagttttat agttagtgta caataaaagt atgtttatac catatggtgt ccggataatc
     2401 agtcatttaa acaatgaggt tcaaagatat gaaaagggga aatttgatga agaaaataag
     2461 caatgaaatc caaattctgg cagaaacttt aactcttccc aaagtcgtta tagagcaaat
     2521 gattgtacaa gcaaaaaaca tccttgatga ggtgtttgag caatatagtc atgaactaat
     2581 ggatcctaat aaagcaaagg cgactgcaaa gaaaatagac aaagtctatg aagaggaacc
     2641 ttttgcagca ttagctattt atctatatgc agcttctcaa agttggaaaa aaatttatca
     2701 accattgaag attccaagaa ctatctattt ggcaacaatg aatgctttta ctagatttat
     2761 tcatgaacat ttccaagtaa caggaagcta tcgctttgac cgtggttttt ggatttggca
     2821 ttacactagt ggcttgatct ttcggattgg tgaattggag tacgaaatga catattttcc
     2881 gaaagggtac acggatctaa gattagaagg aaagcagtgc ctttctcttc atattccttc
     2941 cgatgcagat ttatctcaga ataagataag agatagttac gaaagtgcca ttcgtttttt
     3001 tgaactttat tttcctgatt accattacca agtgatgtac acagatactt ggttactatc
     3061 acccaatctt actaaatggt tgaaaaaaga gtcaaaaatc tgtctttttg cagccgatta
     3121 tcgattatta tcagttgatg aacaggacga tagtggagtc ccttggatct ttggacgagt
     3181 agatgcccaa atacatgatt atccagaaag tacgagttta cagcgacaag caaaagagca
     3241 attgcttgca ggcgagcaca ttggctctgg cttaggtatt cttacttcat aagtttagta
     3301 cttcgaaata gtttaaaaga gcgtgagaca aaagtaaaga acacttttgt ctcacgctct
     3361 aaacacgtat aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa
     3421 aaatttgaaa agcaattttc gtgaatttct tcttatttct tggagttaaa cgcttctgtc
     3481 ccagcctctt ttttagtgtt ctatcgaaca cacagtacgt tcaataggta aactagctat
     3541 aacaatagat attaaagtta acggacaaga gggccttttt taggtagcta aaacgtaata
     3601 atgaaaattt atttccagag ataggagtca atcagctagt ctgatcgact gttttaacaa
     3661 gttgactttc attttaactt ttgataccat ttaaaacaca aatcgtaatt gtttcgattt
     3721 gtgtttttta gcatgactac aatcaaagaa gaaagtgggt cgtccatatg gaaaaaacag
     3781 atatctcaag tgcataccgc cgcttgaaaa gcccaaatat caaaacacga aaacgtgcgc
     3841 tgaagatcat caaagatgtg aaacggaata gtgggaaaag gtaagaagaa ggaaacttaa
     3901 ttaaaaaaat agccgaaaaa cgtttttcgg ctattttcaa taatcggttt ttgtgtaagg
     3961 tttccgaaag aactgctgtt gattttccat ttttaacata tgataaattg gtagaatctt
     4021 tcttttcaaa ttgataccat tccctaattc cagttatata gttaactaca gaaccacaaa
     4081 gcacgtacgt ttataaaagt attatctgta gttatttttc aattcccttt caacctctta
     4141 ttttgaccag tagtattgat cactttacct tttaacacct ttgttcgttt ttgaaacatt
     4201 tttagtcaag aatggaaacg taactacata atcaaaggtt ttgcgaaata tttcatgaaa
     4261 tatttcatgt agaagcgagc aatacggttg caaataacca ttatcgttta ataagcagtt
     4321 acgaaagctc ccaatatctc taacgttcag ctcactccat ttcttttctt caaaaaaatc
     4381 agtgattcgt atctctttct atttgtcgaa aaagttaact ttaaaaagta acgccgaaaa
     4441 aatatgcaaa aaaataaaaa agcagttact attgctagaa taacatcaag gaataataag
     4501 ttttggtttt cagataatag tcgataagtt gtgtattgga attttagata cagtaataaa
     4561 gtatttttca tgaaattttg tgagaatcac tattacaagt atctttaata aaattaatgg
     4621 ttaaggagga acttaaagta gaatgtaaat gaatagttgt agtcagtcga ttgtgatact
     4681 agactcaaag ttgcaggaat aagtttttca atttgtgatt ggaagataaa aaatgaaaaa
     4741 gaagggcttt tttttatact ttgttgattg tagtgtttat tttccctctg tttagccaag
     4801 taacattttc tatgactcaa ccagtttcaa gtgaggaaca tgataacctt tttcgtgcta
     4861 cagaagagtt taagaaatat caggaaaata ttgttccgga gactccatta gctagtgagc
     4921 caattactga tattgatgga aaccaaatag gatggtatat acagtacgaa gttatggtgg
     4981 taaataatga ctcaatagta aaaaatacag taggcttaga gctaacaccg attgaaaagg
     5041 tgagttcatt gttaacgtat atttatactg atgaaagtga cgagactgag atcattttaa
     5101 cggattactc aaaaattgct actgataaaa agtatatatt gtggatgtag acactagtga
     5161 aatactttat gatatagaaa ttgatgaaaa tttactagat gtagcacaag aaatcgatca
     5221 tcaaaagcag gaagctattc gatatgctgg tagaaaaggg aaaagaaaat atttattacg
     5281 tgaatgtagc aaaagttaga gaagatgaaa atgagtggaa agaattcaag agtcgctact
     5341 caattaactc gactccgaca tttacagttt acagagaagg atcgatagaa aaaactgttt
     5401 tttggactaa agaaagtgga atgtctttag ctgaagtaga agagtttttg gattatgtta
     5461 gtatgcaaca gtaagggagt tcttattatg aaaaattttg acacatatta tgttattgtt
     5521 ttttcgatat ctttgctgtt aatattacta actttaatgc agtataaaga gtatccagag
     5581 tattttattt taacttttgt atatttagta agtagctaca cacttctata taaattcaga
     5641 agttcttcta gagtttctaa agtttgtttc ttaattagct tattgattct ataatatttt
     5701 tgatttatat aggaaattaa aaatattata gaatcaacac tcagagaacg ggttggtttt
     5761 taatagattt aataagtact gataatgaaa tttctcctgg ttgggaaaac gaagttggtt
     5821 accctgtaga agaatttttt caagattact ttgcggagta tgccgatagg ataatgagag
     5881 attacgtaac agataaaact caagaagtca tcaatcaatc ccagtcatta aatgaattat
     5941 ctgataaggc gaaagaagaa gcagttgcca ttatttcttc agggattagt gactatattg
     6001 atatgagtgg tgaaatcaat tatggcgata aagacaaaat tgtacgcatg ataaaagctt
     6061 ttatcaaaac ttttgatgtg aaggcagatc ttaagtttaa tgatacttac ctagtaggaa
     6121 aattaattaa tatccttggg acagagaaag atacggaaac gtttatttcc tctttgtcta
     6181 tgttgtttac agataagcat ttttcaactc agcttggtgg agaggaacta gtggaaatct
     6241 tttaaccatt acgcccagca ttaataaaag tatatcaaaa aagtactcct gatgattttt
     6301 aagattggtt tgagaaataa tgtgaaaaca ctatttttaa tgttaattat agatagagat
     6361 gaacgcgtca tatggttaaa agaatgaaaa agtcaaagat acactgctat atttcactaa
     6421 ggatatagca gtgtatcttt catgtcgttt cactggcagt tttttataaa aggaggtgtg
     6481 catttcagtt gcttttcttt ggtagttagt tgaatcgttt aagaattaac ggatgtggca
     6541 gacaggtgct ctgtccaaaa accagtaaaa tctttgatgg ttccatcttt atttagctgt
     6601 gcctgttcaa atactgtttt gtttttaccg aatggatcaa ttgcaataat tgttccgatc
     6661 cccgcacgtt tggcagcaag taatcctgag taagcatctt cgataacaag acaatcagcc
     6721 ggagctaagc tcagtttgtc tgccgcttta agaaaaatat ccggttgcgg tttcccaggg
     6781 aaagagccgt cgtcatagac aatcttgtcc cagtcaaacc agcgggctaa atcgaaaata
     6841 tcaaaataaa aagcaacatt ctctttgaca gtagccgtgg caatcgttaa tggaatctgt
     6901 tggtcagtca gctggtcaag agtcgcttca agcccttttg tcaactgaag ctcgttttca
     6961 ttttgcaagc aaagcatacg ataatagctt tctttttctt ctgacaatac ggcgatctca
     7021 gaggcggtca aatgctctga aatgaaatgt ttcaaaatct ccgtattcgt ccgaccatga
     7081 atatttttta aaatatcctc gtctgataaa ccatcttttg tatgatcatg tatcatatgc
     7141 agccaagcag cttcatgcag atgagagtcc aaaaacattg ttccgttgaa atcaaatata
     7201 atccctttca tagttacgct cctatagtgg tattttcggt tgaatcagca atttgcagat
     7261 cgacatattg tgcatagtgc tcttgaacct cccgcgagat ctggcgatcc gtaatgagca
     7321 gatcgaaatc atgcaaatta taaaacacat aaaaatcatc tcgattgaat tttttattat
     7381 caacgagtag atatttctta cgggaattat ttagagcgat ctgctgtgct tctccttctt
     7441 cggtactgta agtagagatc gcttcgttgt gaacgccatt tgaactgata aatgcttttg
     7501 taaactttag tcttcttaaa ttgtcgttgg tgatcggtcc gacaaatgcg ccggtatttt
     7561 tccggaaatc accgccaatc agtagaattt ctggtccatc tgcttgagca agaatatcaa
     7621 aaactggata attatttgta atgatccgaa ttttcttgtt cagtaaatgc gtcgccaata
     7681 gttcgattgt tgtacctggt cctaagaaaa tcgtttctcc ctcgtgaatc aaggaggctg
     7741 ctgtggcagc aatttctttt ttttcttgaa tctgcagagt ggctttttca gtgtgagaaa
     7801 gttcttgatc taaagtgtag gaaagacttt gagcccctcc atggacacgc agcagcttgc
     7861 ccgatttttc taattcatct aaatctctgc gaacggtcat atcagaaaca tctaattgat
     7921 tgatgatttc attaacggta aggattcctt ttttattgac catttctacg atagtaagca
     7981 atctttcttt tttcaacatt gtcttcacct ctaatttaag gataatctat catataaacg
     8041 gacaacagtc aatctaattc gaacaaaagt aaacatttag ggtggttttt atgtttgtta
     8101 caatagattt tattgctgtt ttctggtttt tatgattatt tgtgtttgtt tttgttgaca
     8161 aatgttttgg atgcgtttac aatgcaatca gttagaaaga gcgaaaactg attatagatt
     8221 tgaggagaga tgtacatgct taaattacca gaagatttta tttttggcgg agccacagca
     8281 gcctatcagg ttgaaggagc cactaaggaa ggtggcaaag gcgctgttgc ttgggatgac
     8341 tttttagagg aacaagggcg attcagtccc gatccggcaa gtgattttta tcatcaatat
     8401 gcaaaggaca ttgaattgtg tgaacgtttt ggtgtaaatg gtctaagatt atccattgct
     8461 tggagccgca tttttcctga tggagctggg aaaccaaatc cagaaggaat cgctttttat
     8521 catcgtgtgt tcgaagaatg taagaaaaga aacgtaacgc cgtttgtgac gttgcatcat
     8581 tttgataccc ctaaacgttt gtttgaccat ggagattttt tgaatcgaga aacaatagaa
     8641 gcatttgttt cctatgccat tttttgtttt catgaattca aagaagtcaa ggtgtggagt
     8701 acctttaatg aaatctatcc agttgcaacc aatcaatatt tgttaggtgt tttcccacca
     8761 ggaatcaaat atgattttac aaagatcgtc gcctgtcttc ataatatgat ggttgctcat
     8821 gcccgtgtag tgaactattt caaagaaaac gaacttccgg gtgaaattgg tgtagttcac
     8881 tcattagaga cgaaatatgc ggcaacagac gcgccggaag ataagcatgc tgccttttta
     8941 gacgatgcat tgtcgatccg tttcttatta gatgctacct atttaggcta ttattcaact
     9001 gaaaccttga ctgctcttga tgagatatgt gaagcaaatc aggccagcta tcattttcct
     9061 gaagaagatt tcgttgaatt gaaaaaagca tccacgcgta atgactactt agggatcaat
     9121 cactaccaat gccattttgt caaagcttac gatggagaaa atgcaatcca tcataacggt
     9181 acgggtgaaa aagggacctc tgtttataaa gtgaagggaa tcggagaacg tatttataaa
     9241 gaaggaattc ctcgaactga ttgggactgg ctgatttatc cagaaggtct atatgacttg
     9301 ttgctgcgaa ttaaaagcga ttacccacat tacaacaaaa tctatatcac tgagaacggg
     9361 atggggtata aagatcagtt tgaagatggc attattatgg atcagccgag aatcgactat
     9421 ttgagagtct atttggaatc cttaagcaag gcgataacag ccggggtaaa tgtgaagggg
     9481 tattttttgt ggtcgcttat ggatcttttc tcatggacaa atgggtataa caaaaggtat
     9541 ggcttgtttt atgttgattt tgaaacccag aagcgttacc caaaagaatc agcttactgg
     9601 tacaagttgg tcagtgaaac caaaacgatt atttaataag tgataatgga ggaagaaaag
     9661 atgaataaag aggaattaca aatgcttggt tttgagatcg ttgcatattc aggcgacgct
     9721 cgcagtacat tattgaaatt attgaaagaa gtaagacaag gaaactttga acatgttgat
     9781 tcggcgttga aagaagccga cgaaaattta acccttgcac ataattcgca aacaaaaatt
     9841 ttagcggaag aagcatctgg gaaagagatg gagatgggat tcatttttat ccatgggcag
     9901 gatcatttaa tgaccacttt attgctgaga gatctgattc aagactttat tgtactgtac
     9961 agacagaatc aaggatagga gaagaacgct atggatggaa tcattaaaca aatcgaaaaa
    10021 gggaaaccat tttttgaaaa aatctcacgt aatagttatt taggagccgt tcgtgacgga
    10081 tttttaacag cgatgccagc tattttattt tcaagcattt tcattttgat tgctgcggtt
    10141 cctgagattt ttggttacca atggcctgaa tctgtctcca catggctatg gaaagtctat
    10201 aattattcga tgggtgttgt agggttactc gtttcagcaa ccactgctcg ctgcttggct
    10261 gattcgatga atcgtcgctt accgaaaaac aaagtaatca acaccacctc tgtgatgctg
    10321 gcctctgtgg taagtttcct gttgttaagc gtctctccga ttgaaggtgg attttcaacg
    10381 gaatatatgg ggacaaaggg actactggct tcctttgtcg ctgcattttt aacagttaat
    10441 ttatataagt tttgtgtgct gaaagatatt accattaaaa tgcctaaaga agttcctgga
    10501 acgatttcgc aaaccttccg agatattttt cctttctctt tttcagtatt tgcggccgtt
    10561 ttaattgatt taggcattcg acaaatcttc caaatctcat ttgcagaagc aatcattacg
    10621 gtgctgcagc cgctatttac agcagctgac gggtacttag gaatcgctat tatctgggga
    10681 gcgatggcgc tgttttggtt tgtcggtgtt catggaccgt cgatcgtgga gccggcaatt
    10741 gcggcaatta tctatgccaa tgtggaaacc aatctgcaat tgttcaattc cggtgaacaa
    10801 gcttctaaag tactgacggt gggtttaggg aattttgtag gaactatggg aggaactggg
    10861 gcaacattgg ttgttccttt tatcttccta ctctttgcta agtctaagca gttgaaggca
    10921 gttgggaaag cttcctttat tccagtaagt ttcgctgtca acgaaccatt gctatttgcg
    10981 gcacccatga ttttgaaccc ttattttttt attccatttt tgttaacacc tgttgtaaac
    11041 gtttgcctgt tcaagttttt tgtcgatgta ttgggcatga acagctttat gtacgttttg
    11101 ccttgggcaa ctccagcacc gatcggtttg gttctaggaa cgggcatggg tatcttagct
    11161 tttgttctag cagcggttct aatcgtcgtt gacttcctga tctatctacc attttgcaaa
    11221 gcatacgatg ctgtcttagt agagaaagaa gcacaaaaag cagaggtgat cgcaaccgag
    11281 gaaggtgctt ctgccgatgt acgtgaggaa gtacctgtgg cagcgagtca agttatcagc
    11341 gatgcaaata aagaaatcaa tgtgttggta ctttgtgctg gcgcagggac aagtgcgatg
    11401 ttagctaatg ctttaaaaaa aggtgcatct gaatcgaaag caccgatcag tgcaatggcg
    11461 ggtgcttatg gttctcatta cgaaatgctg caggattttg atatggtggt cttagcaccg
    11521 caagtcaatt cttactatga tgatatcaaa gaagacaccg atcgcttagg aatcaagctg
    11581 gttgcgacaa aaggagcaga atatatcgga ttaacgagag atcctgaaaa agctgttcag
    11641 tttattctga accaatttca ataaaggagg agccataatg aaaatgggaa tttggttgcg
    11701 taaagctgtt ttatttgcag ctgtgttagg ggctttgggg gctgtttcaa aaactgttga
    11761 agcatctgcc agcgaattta ttcgcggagc agatatttcg atcctgtcag atatggaaaa
    11821 atctggtgca aaatattatg aagacgatat ttcaaaagat gccttagaga ttttaaaaaa
    11881 taatggtgtc aactatgttc gactgcgttt gtggcaggat ccttataata gtaatggaga
    11941 aagttatgga gcagggacaa atgatctgga aacgacgatt gaccttgcaa aacgagcaaa
    12001 aaatctggga cttaaggtgc tgctggattt tcactacagt gatttttggg ttgatcctgg
    12061 gaaacagaat cttcctaaag catggcaagg tctgaccttt gaagaaatga atacggcatt
    12121 gtacgactat acaaagaacg ttctgtcgga aatgaagcag ctggacgttt atcctgatat
    12181 ggttcagatc ggcaatgaac tgaatagcgg aatgctctgg ccttatggca agtcttgggg
    12241 agaaggcggg ggagaatttg atcgattggc tgcttttttg aagtctggta tccaagctgt
    12301 tcgagacact caacctaaaa caactccagt aatgctccac ttagctgatg gtggcgatac
    12361 aggagcattt acttggtggt ttgatgaaat cacgagtcga ggtgtgtcat ttgatttaat
    12421 tggagtgtcg tattatccgt attggcacgg ttcaatgaat gaacttcaaa ccaatatgaa
    12481 tgcgatcagc caacggtatg gtaaagaagt gatcgtagtt gaaacagctt atgggcatac
    12541 aacagcgaat gccgatacta tgccaaatgc ttttggtgaa gccgaagcag ctgcgggggg
    12601 atatgaacca acaccgacag gacaagcaga gtatttgctg gatcttgccg atcggattca
    12661 ggcagtaccg aataatcgcg gggcgggctt cttctattgg gaacctcttt ggtacaacgg
    12721 aaatgtgagt tgggcaacac aagcaggtat gaattatctc agtgttcaat ccactatggg
    12781 aaacgaatgg gacaaccaag cgatgtttga ttttgctgga aacgccttgc caagcttgcg
    12841 tgcgtttaaa caagcagggg cgcaaataaa tctagttaaa aatgcaagtt tcgaaagcga
    12901 tggaccaacg accagtcctt ccaattggca aacctggttt caacaaggaa ccgctaaggg
    12961 tacgatcaaa acggaagcgg gcactttttt tggggactac aaactaactt tttggcagga
    13021 tcaagcatac gaggcatcag cttatcaaaa tgttagtggc ataagcaaag gaacttacac
    13081 attgagtgcc tatgtaatgt ctgggggaga gtttgatact aatcagttat atatcaaaaa
    13141 ttatggtgga gcggaactaa atactgcggt tgttaaaaca agtgaatgga caaaaattac
    13201 aatcaaagac attccaatta caacaggtgt ttgtgaaatt ggggtttata gcaaggcttc
    13261 aggaaatgct tggacgaacg ttgattttgt ttcattggtg aaacaataga ctgcggatcg
    13321 tttcgattca agatcggagg ttcccttcag ccacatcgtt agcgtagaag gcaggaatcg
    13381 aacctgcacc tcgcctttgc caaaaataac cagaaacgat ttccagtcat ttttaccaaa
    13441 acgagtaaag caccagactt catctacact ttgtattatg ctaaagagag ttcaacgatt
    13501 tcgtaagaag caatgaaaaa attgcaaaaa gttgtgcaga taacataaag tttgtttaag
    13561 aaagggtaac cttagaattc cgtcgtgaat atgatttgag cacttagatg gatagaaaaa
    13621 agttctcgga aaacagttcg tttttcgaga acttcttttt atgataagaa tgaaacaaat
    13681 taacttatat tatagtctct tttggtaggg aaccatctta tttttaatga aaatcaagca
    13741 ttttagctat tgaagaatgt tgatttatag ggattccccc tccatattat gttggctctt
    13801 tattcccact ctgttttcaa cgtaaagtgt ataaaacaga atgc
//
LOCUS       DACDSY010000074        13829 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDSY010000074 DACDSY010000000
VERSION     DACDSY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.075"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..106
                     /locus_tag="IWK44_001782"
     CDS             <1..106
                     /locus_tag="IWK44_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9550286.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            255..593
                     /locus_tag="IWK44_001783"
     CDS             255..593
                     /locus_tag="IWK44_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550287.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(798..1373)
                     /locus_tag="IWK44_001784"
     CDS             complement(798..1373)
                     /locus_tag="IWK44_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP9550288.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2105..2686)
                     /locus_tag="IWK44_001785"
     CDS             complement(2105..2686)
                     /locus_tag="IWK44_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9550289.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2869..3495)
                     /locus_tag="IWK44_001786"
     CDS             complement(2869..3495)
                     /locus_tag="IWK44_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9550290.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3517..4848)
                     /locus_tag="IWK44_001787"
     CDS             complement(3517..4848)
                     /locus_tag="IWK44_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP9550291.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5003..5404)
                     /locus_tag="IWK44_001788"
     CDS             complement(5003..5404)
                     /locus_tag="IWK44_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9550292.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5490..5696)
                     /locus_tag="IWK44_001789"
     CDS             complement(5490..5696)
                     /locus_tag="IWK44_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550293.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5791..7293)
                     /locus_tag="IWK44_001790"
     CDS             complement(5791..7293)
                     /locus_tag="IWK44_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP9550294.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7311..7766)
                     /gene="argR"
                     /locus_tag="IWK44_001791"
     CDS             complement(7311..7766)
                     /gene="argR"
                     /locus_tag="IWK44_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9550295.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7864..8481)
                     /locus_tag="IWK44_001792"
     CDS             complement(7864..8481)
                     /locus_tag="IWK44_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550296.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8702..10075
                     /locus_tag="IWK44_001793"
     CDS             8702..10075
                     /locus_tag="IWK44_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9550297.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10150..11262
                     /locus_tag="IWK44_001794"
     CDS             10150..11262
                     /locus_tag="IWK44_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP9550298.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11334..12971)
                     /locus_tag="IWK44_001795"
     CDS             complement(11334..12971)
                     /locus_tag="IWK44_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP9550299.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13139..13747)
                     /locus_tag="IWK44_001796"
     CDS             complement(13139..13747)
                     /locus_tag="IWK44_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9550300.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3744 a         2903 c         2474 g         4708 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagaaacta
      121 aaaaaataat ccaagaaaca ttgaacctat ggatccaaaa gctacccaaa tactatagct
      181 aaccccccgc taccttcaat tttgcagata aaacgctttg ggagaaaatt tcaaagacaa
      241 caggccatcc tatcatgccc gattataaac aaaaagctgg ctattccttt tgctctgaca
      301 aatttcaatt gggaaagttc acgaaactag caggtgaaga agtttcaact gttcggatca
      361 aagaatgccc gcttcaaata gaaacattgg ttcaacgaat ttcaactaga caagactttg
      421 ctattgtaga acgtgagatc gtagctattt ttgtagaaga agggatttta catgatccaa
      481 ctcatatcaa tgtggataaa tggcaacctc ttatctataa attcagagaa tatacaactg
      541 ccaaccagtc gttaactttg aattttagat tccaagaatt caaagagtca taatgataaa
      601 catcgttagg tataaataaa aaagactcgc agcacgagta aaaaacaaat agaagctata
      661 cattagaaag gtcgactgtg accctataga tgcagtatat gcagcttatg agcatttctt
      721 ctgttctctt tgtttttggg atctgtgctg aagtcttttt cgttttttgc cgctctttct
      781 ttgaaaaatc gattatgtta taccttctcc attactaatt caggcatctg ctgagtaatg
      841 atttttcgcg caaaagaaat atctgttacc caaggttcga tttgttcttc agtcaagaca
      901 agcggcattc ggtcatggat tggtgcgact gtgcggttag gttcagtagt cattatgatt
      961 gattctgcca ttttttcatc ctgatggcta cgataaaaac cgccaatata cactattttt
     1021 tccttatttg aaaacataaa tttgtctttt tcttcattcc actcataaaa gccactcatt
     1081 gggaaaacac aacgattttg caaaaatggt tgatgaaagg tctttttttc ttctactgtc
     1141 tctgcacggg cattaatcat caattgtcct tttttaaatc cggtaaatcc ccacttcatg
     1201 atttccggag tgaggatgtt ttcttcctct tttcttaacg tcacaacaag atttgacgga
     1261 aaaacttctc cagctgctaa ttctgctgcc tgcttcggtt gtttcgtctg ggcttctcga
     1321 taataattct gaaggagctc tgactctaga tcaaaaaaat aacgaccaca catcctaatc
     1381 cctcccaatc tttttttcta tcatacactg caacaagaag ttcgcaccaa caaaacgctt
     1441 tcaatttttg tagaactaaa aaagttgtga caagtgacaa actctccagt gaatggtatt
     1501 ctcctgccaa ctcctcggtc acaagtcaca atattcaaca aacatgagaa gctgcttttt
     1561 gaaattgttc cttgtgactc ggagtgaaac ggcaggctta cgacctctgg gtaaacggtg
     1621 ttcaaaagcc agtttctcgg tattaagcca aatttatgca aaaatgtaga aagccatttt
     1681 cacaaaattt ttcttaatac tcgaaactaa gcgacttttt cacaacctct tccttaaacg
     1741 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata tgagcagcta
     1801 tttttatttg gctgttctta ttacttgatg cagggcagct ttttcacaac ctctccacta
     1861 aacggtgttc aatcagctaa ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac
     1921 tattttgccg tttgtttctc taatactcag gtcaaagcgc tgatttcaca acctctaata
     1981 cacggtgttc cagaagtagc ttcttcgaaa aataagccga aatttcacaa aaatttgaga
     2041 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctcta
     2101 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
     2161 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
     2221 tcaacgcttt ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca
     2281 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
     2341 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
     2401 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
     2461 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
     2521 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
     2581 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
     2641 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
     2701 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
     2761 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
     2821 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
     2881 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
     2941 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
     3001 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
     3061 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     3121 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     3181 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     3241 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     3301 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     3361 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     3421 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     3481 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     3541 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     3601 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     3661 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     3721 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     3781 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     3841 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     3901 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     3961 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     4021 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     4081 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     4141 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     4201 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     4261 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     4321 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     4381 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     4441 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     4501 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     4561 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     4621 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     4681 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     4741 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     4801 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     4861 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     4921 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     4981 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     5041 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     5101 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     5161 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     5221 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     5281 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     5341 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     5401 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     5461 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     5521 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     5581 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     5641 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     5701 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     5761 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     5821 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     5881 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     5941 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     6001 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     6061 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     6121 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     6181 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     6241 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     6301 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     6361 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     6421 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     6481 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     6541 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     6601 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     6661 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     6721 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     6781 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     6841 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     6901 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     6961 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     7021 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     7081 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     7141 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     7201 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     7261 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     7321 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     7381 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     7441 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     7501 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     7561 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     7621 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     7681 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     7741 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     7801 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     7861 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     7921 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     7981 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     8041 taagatttgg gcacaaagac cttcttccat ctttttgaat actagttgtt ctttcaagtc
     8101 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     8161 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     8221 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     8281 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     8341 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     8401 tggtttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     8461 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     8521 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     8581 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     8641 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     8701 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     8761 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     8821 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     8881 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     8941 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     9001 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     9061 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     9121 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     9181 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgattc tttctttgat
     9241 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     9301 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     9361 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     9421 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     9481 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     9541 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     9601 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     9661 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     9721 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     9781 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     9841 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     9901 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     9961 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
    10021 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
    10081 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
    10141 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gactctagcc
    10201 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
    10261 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
    10321 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
    10381 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
    10441 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
    10501 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
    10561 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
    10621 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
    10681 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
    10741 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
    10801 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
    10861 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
    10921 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
    10981 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
    11041 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
    11101 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
    11161 tatatcggct atctattagc caacttctta aatactggtc attggtctac ggaagattat
    11221 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
    11281 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
    11341 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
    11401 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
    11461 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
    11521 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
    11581 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
    11641 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
    11701 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
    11761 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
    11821 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
    11881 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
    11941 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
    12001 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
    12061 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    12121 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    12181 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    12241 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    12301 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacagccgct
    12361 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    12421 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    12481 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    12541 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    12601 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    12661 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    12721 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    12781 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    12841 gctgtcttcc tgtcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    12901 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    12961 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    13021 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    13081 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    13141 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    13201 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    13261 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    13321 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    13381 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    13441 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    13501 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    13561 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    13621 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    13681 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    13741 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    13801 catttagaaa tatttcatag gtaaaagaa
//
LOCUS       DACDSY010000075        13643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDSY010000075 DACDSY010000000
VERSION     DACDSY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.076"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..412
                     /locus_tag="IWK44_001797"
     CDS             <1..412
                     /locus_tag="IWK44_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550301.1"
                     /translation="YTDTKTIREYFGIEYSSNLGDVLFQFSQTLARFIPTEVSEKKNE
                     DQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKRSPFNDNKTRLYRPSLYER
                     LGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            484..879
                     /locus_tag="IWK44_001798"
     CDS             484..879
                     /locus_tag="IWK44_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9550302.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            863..3316
                     /locus_tag="IWK44_001799"
     CDS             863..3316
                     /locus_tag="IWK44_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9550303.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            3313..5340
                     /locus_tag="IWK44_001800"
     CDS             3313..5340
                     /locus_tag="IWK44_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550304.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            5337..6359
                     /locus_tag="IWK44_001801"
     CDS             5337..6359
                     /locus_tag="IWK44_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9550305.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            6376..7305
                     /locus_tag="IWK44_001802"
     CDS             6376..7305
                     /locus_tag="IWK44_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9550306.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            7550..7666
                     /locus_tag="IWK44_001803"
     CDS             7550..7666
                     /locus_tag="IWK44_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9550307.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            7682..8485
                     /locus_tag="IWK44_001804"
     CDS             7682..8485
                     /locus_tag="IWK44_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9550308.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            8660..8956
                     /locus_tag="IWK44_001805"
     CDS             8660..8956
                     /locus_tag="IWK44_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550309.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            8963..10915
                     /locus_tag="IWK44_001806"
     CDS             8963..10915
                     /locus_tag="IWK44_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9550310.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(10987..11484)
                     /gene="dfrG"
                     /locus_tag="IWK44_001807"
     CDS             complement(10987..11484)
                     /gene="dfrG"
                     /locus_tag="IWK44_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9550311.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            11846..12886
                     /locus_tag="IWK44_001808"
     CDS             11846..12886
                     /locus_tag="IWK44_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9550312.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            12984..13172
                     /locus_tag="IWK44_001809"
     CDS             12984..13172
                     /locus_tag="IWK44_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9550313.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(13230..13583)
                     /locus_tag="IWK44_001810"
     CDS             complement(13230..13583)
                     /locus_tag="IWK44_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9550314.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
BASE COUNT         4634 a         2300 c         2604 g         4105 t
ORIGIN      
        1 atatacagac acgaaaacaa taagagaata ttttggtatc gaatacagta gtaacttggg
       61 agatgtttta tttcaattta gccaaactct tgctcgattt attccgacag aagttagtga
      121 gaagaaaaat gaagaccaaa aagaagccat gtgtttctca ttaagtcaaa gtgattcaga
      181 agatcccaga aaaaaatatt gtttttcagt tagaagaaat cctttaaagg gaaatggtga
      241 gctgggaaaa cgaagtcctt ttaatgacaa taaaacgaga ttgtatagac ccagcttata
      301 tgaacgatta gggtcagata ccaatttaag tttcagatat tcaatgaatc cagatgatga
      361 agaaactgat gaaggtatta ttgcaaaatg gacaaaaaat aaaattgaat agttaaagca
      421 ttgaaagagc aatcacctat tttgattgtt ctttttctat ttttacggaa aggacgtgaa
      481 gacatgaaaa aaataagaag ttacaccagt atttggtcag tagaaaaagt gatttatgcg
      541 attaatgatt tacaactccc tttcccaatt acctttacgc aaatgacatg gtttatcgtt
      601 tctttgtttg tcgtcatact ctttggcagt ttaccaccac tttctctgat tgacggagca
      661 ctactcaaat acttagggat tcccattgtt ctcactgggt tcatgtgcca aaagaccttt
      721 gacggaaaga aaccctatgg atttctaaag tctatcatta cctatgctct acgccctaaa
      781 gtaacctacg ctggcaaaca aatacgatta aaaaaagaaa caatgaatga atcgattacg
      841 attgtaagga gtgaacaact agatggcata tccgattaaa tatatggaag ataatctcgt
      901 atttaatcat gatggcgaag tattcgctta ttatgaatta attccctata attacagttt
      961 tttaagttcc gatgaaaaaa ttcaagttca tgataatttc tgtcaattga ttgcccaaaa
     1021 tcgtgatggc aagattcatg ctctgcaact agcaaccgaa tcaagtattc gagccacaca
     1081 ggaacaatcg aaaaaagaaa tcactggacg attaaaagag atagcttacg aaaaaataga
     1141 tgaacagacc gaagcgttaa tttctatgat tggtgacaat caagtagact atcgcttttt
     1201 tattggtttt aaactctacc tcaatgaaca ggaagtatcc gtaaaaaatg ttgggaaaga
     1261 aattcaaaaa gcatttgcgg aattctttca tggtgtgaat cacaaattaa tgggcgattt
     1321 tgtttctatg cctatggcgg aaatcgaacg cttttctaag atggaacagt tgttagaaag
     1381 taaattatcc agacgctttc agattcgtaa attggagaaa gatgattttg gttatattat
     1441 tgaacatctt tacgggcaaa caggtgtccc ttttgatgac tacgactacc atttaccgaa
     1501 acgatacgaa gataaaaaag tgttggtaaa gaaatacgat attctcaaac ccactcgctg
     1561 tttaatcgaa gaaaatcaac gctatttacg gattgaacag gaagatacca ctacttatgt
     1621 ggcgtatttt acaattaata gtattgttgg tgaattggat ttccctaata gtgaaatttt
     1681 ttattaccag caacagcagt ttactttccc cgttgatacc agtatgaatg ttgaaattgt
     1741 cgccaataaa aaagccttat ctaccgttcg caataaaaag aaagaactga aagatttaga
     1801 tagccacgca tgggaaagcg acaacgaaac cagtactaat gtggtggacg ctttagaatc
     1861 tgtcgatgaa ttagaaagta atttagacca aaccaaagaa tctatgtaca aactaagcta
     1921 tgtggtacgg gtatcagcaa agaatttgga cgagctgaaa caacgttgtg atgaagtaaa
     1981 agatttttat gatgatctgt ctatcaaatt agtacgacct tttggcgata tgctaggctt
     2041 gcataatgaa tttattccct caagcaagcg atatatcaat gactatattc aatatgtcac
     2101 gtcggatttt ttggctggtc ttggtttcgg tgctacacaa atgattggtg aaaattctgg
     2161 tatttacgtc ggttacaatc tcgatacagg cagaaatatt tatctccaac ctagccttgc
     2221 aagtcaagga attaaaggct ctgtaacaaa tgcattagcg tccgctttta ttggttcatt
     2281 aggtggcgga aaatcattct ctaacaatat gctggtctat tattcagtac tctttggcgg
     2341 acaggcaacc attgttgacc caaaagctga acgtggtgaa tggaaagaaa ccttacctga
     2401 tattgcccat gaaatcaata ttgttaatct cacgagtgag aacgacaata aagggttact
     2461 tgacccttat gtgattttaa aactcccgaa agattcagaa agccttgcca ttgatattct
     2521 cacgttctta acaggtattt ccagccgaga tagcgagaag tttcctgtcc tgcgtaaagc
     2581 tattcggagt gtaactcaaa gcaaacaacg agggttgtta ttggtgattg atgaattgcg
     2641 aaaagaagat acgcccatca gcaatagtat tgctgaccat atcgaaagct ttgtcgatta
     2701 tgattttgcc catctgttat tcagtgatgg cagtgtcacc caatccatta gcttggaaaa
     2761 acaactcaat attattcaag tggcagattt ggtcttaccc gattccaata gctcttttga
     2821 agaatacact accatggaat tattaagtgt ggcaatgctg attgtcatta gtacctttgc
     2881 tttagacttt atccactctg accgttccat ttttaaaatt gttgaccttg atgaggcttg
     2941 gagtttttta caagtggcac aaggaaaagc tctatctatg cgattggttc gagcaggacg
     3001 tgccatgaat gcaggggtct atttcgttac tcaaaacgcg gacgatttac tagatgaaaa
     3061 gatgaagaat aatattggct tgaaatttgc ctttcgttcc actgacctag tggaaatcaa
     3121 aaagacctta gaattttttg gtgtggacaa ggaagatgaa aacaatcaga aacgcttacg
     3181 tgaattagaa aatggacaat gtttagtttc agacatctat ggtcgtgtag gggttatgca
     3241 gtttcacccg atttttgaag agttgcttca tgcctttgat accagaccgc ctgtgcgaaa
     3301 tgaggtggaa gaatgacaca aaaacaaaag aaatttcttc actatagttg gataacgctc
     3361 ctttgtgtgg gcgttatttt acttttatta ggcacgctgg gcaatgcagt acaagcgaca
     3421 ggcttagttg atgaaaccat tgatacaagc aatgaatact ccaagtatgg tttgaatcaa
     3481 tatcagcttg attattatgt ggacaatagc tggggctggc ttccatggaa ttggagtgat
     3541 ggcataggac aatcggtgat gtatggattg tatgccatta ctaattttat ttggacaatt
     3601 tctctttatc tatcgaacgc cacaggttat ttggtgaaag aagcctatgc cttagatttc
     3661 attagtcaaa catcccaagc aattggcgaa aacatgcaga cgattgcagg cattacaccg
     3721 aatggctttt ccagttcagg gttttatgtc ggtttcctcc ttctttttat tctcattgtt
     3781 ggaatctatg ttgcctatac aggattggtt aagcgagaaa cgacgaaagt gatacgagct
     3841 gttttaaact ttgtcgtggt gtttattcta tcggcttcct ttattgcgta tgcacccgat
     3901 tatattgcga aaatcaatga tttttccaaa gatgtcagta ccgccagtct gaatattggg
     3961 acaaaaattg ttcttcctaa cacggatagt caagggaaag acagtgtgga catgatacgt
     4021 gacagcttat tctctgttca agtaaagcaa ccatggctat tgcttcaata tggctccact
     4081 gatgtagaag cgttagggga agaacgtgtg gaaagtttat tgtctaccag tccagataca
     4141 aacaatggtg aagatagaga aaatgtagtg attgaagaaa ttgaagatca tgacaatacc
     4201 tatttaaccc taccgaagac gatttccaga ttaggaacag tcttcttttt gtttgtattc
     4261 aatattggta tctcgatttt tgttttcctt ctaacaggaa tcatgatttt ttcgcaagtg
     4321 ctatttatta ttttcgctat gtttttgcca atcagtttcc tattaagtat gttgccgagt
     4381 tttgatggca tgggcaggca tgccattacc aagttattta atgtgattat gacaagggct
     4441 ggtattacct tgattattac cgttgccttt agtatttcca ctatgcttta ttcgctgtca
     4501 gcaagttctc cctttttcat gattatgttt ttacaaattg tgacctttgc agggatttat
     4561 tttaaactgg gcgatttaat gagcctattc actttacaaa gcaacgactc tcaaaacatg
     4621 ggcagacaag tgtttcgcag acctcgtcaa atgatgaatc gacaaaccag aagatttcaa
     4681 cgcagtatga gtaagatttt tcgtggaaaa gcagggtcaa cgaagcaaaa gacaatgaca
     4741 acaaagcaga atcaaaccaa taaagctaac catactcgtc aaaatgaacg gactacacca
     4801 aagaaagaaa cagcactttc caatgtgaaa aagcaacaag gcggtatcgg gcaacgatta
     4861 ggtgaaaaga cagcccatgt attggacacg aaaaatcgtc tagtcaataa aagaaagcag
     4921 gcaaaacaac agatacaggc gacaccaacc catgtcaaat atgcccttca tcaaggaaaa
     4981 gagaatgtca ctcataatgt aacagacttt aaacaatcta tggcagatac caaagctcaa
     5041 cgcaaacaac agcgtatgaa ccagcaggag caaaaacgaa agaacattgc tcaaaagcgt
     5101 ttggagctgg ataaagcaaa agaaaaaaga cgaaaagaaa acccatcatc tcctgttcag
     5161 aataaccagc agcatgttcg caagcgtcag caaaccgtca cgcctgtttt gtcacaaaac
     5221 actaaacgtg aaaatcatca gcgaccattg aaaaaagagc aaaaacaaaa gacaccaccg
     5281 aagcaattca atcagaggaa gctaaataaa agaacggata agcgaggaca acaaacatga
     5341 agctaaaaat aacagtgatt tgtagtgtgg tcttctctct cttctttttt ctcttattat
     5401 tcctagtcat tttctttgca gatgatacgg acagtggcga aaacaacaag gattcttcta
     5461 ttccacaagg cggtgtgacc gtttcaccag aagtgttggc acaccgtccg ttgattgaaa
     5521 aatatggtaa ggaatatggg attgaagact atgtttctta tatcttagcc attatgcaag
     5581 tggaatcggg cggtacagca gaagatgtta tgcaaagttc cgaatctttg ggtttaccgc
     5641 ccaacagttt gagtaccgaa gagtccatca aacaaggggt taaatatttc agtgaattat
     5701 taaccagtgc agaacaacaa ggagtggata tagattctgt tatccaatct tataattatg
     5761 gcggtggctt tttaaattat gtgaggagtc atgggaaaaa atatacctat gaattagccg
     5821 aacagttttc taaagagaag tcgggtggtc aaaaagcaga ctatcccaat cctatagcca
     5881 tacctgtgaa tggtggttgg cgatataact atggaaatca gttttatgtt cagttggttt
     5941 cacaatacct aaccgacact tccccaacag aatttgatga tgaaaccgtg caagtcatta
     6001 tggacgaagc cttgaaatac gaagggtttc cctatgtctt tggaggggcg tcgcctacca
     6061 cctcttttga ttgtagtggc ttaatacaat gggtgtatga caaagctggc atttctctcc
     6121 ctcgtgtcgc ccaagatcag tatgacgcta cgcaagaaat ctctatggaa gaagcccaag
     6181 caggcgacct tatcttcttt cattcaacct ataatgcagg cacatatgtg actcatgtgg
     6241 cgatatattt agagggaaat cgcttttatc atgcaggcga cccaatcggt tatggcgact
     6301 taagcagtcg ttattggcaa gaccatctga ttggggctag acgtgttatt cataactaag
     6361 aaaggaatga atcaaatgat tataaaaatc gagcgaaaag aaaagaagga gaaaccaaag
     6421 aaatctaaaa aaccgagcaa gcaaagaaaa gttccgatga ttaaagttgg cacgcataag
     6481 aaattaacct ttgttttatg ggttttattg attggtagtg tcggatttgg aatctataaa
     6541 aattttactg ccattgacac tcatactgtc cgtgaaacag aaatcattaa acagcagatt
     6601 gtcgatacca accaagtaga aagttttgtg aaatcctttg cgaaagacta cttttcgtgg
     6661 caacaatccc aagaagccat tgataaacga aatgaaaaat tgacccatta tttgacggaa
     6721 gaattgcagg tattaaatga agaaatgatt cgtaaggata ttcctactag ttctagtgtg
     6781 aacgatatac aggtgtggca ggtttctcaa gtgaatgaga acacctttga ggtactgttt
     6841 tctgttgaac aagttattac cgaagacaaa gacaaggaaa ctatatcgtc tagctttcat
     6901 gtggttgtcc atatagatga atcagataat atggttatta tcaaaaatcc gacaatgagt
     6961 aagaaacctc aaaaatcgga ctaccaaccc aaacagctag aaagtgacca tacagtagat
     7021 acagaaacga tggacgaaat cactagcttt ttagaaacgt tcttccagct ctatcctacc
     7081 gccacagaaa aagaattgac ttactatgta agtaatcatg tgttacctat gattaacaag
     7141 gaatatgttt tcgaggaatt ggtgaatcct atctttacca gaaaagataa ccaagtaatc
     7201 gtgaatgtcg ctgtgaaata tttagatcaa gaaacaaagg caacccaaat ctcgcaattt
     7261 gagttgatat tagaaaaaca agataattgg aagattgtaa aataacaaat attggtacat
     7321 gattacagat actttgtaat catgtactct ttttgataaa aaattggaga ttcctttaca
     7381 aatatgctct tacgtgctat tatttaagtg actatttaaa aggagttaat aaatatgcgg
     7441 caaggtattc ttaaataaac tgtcaatttg atagcgggaa caaataatta gatgtccttt
     7501 tttaggaggg cttagttttt tgtacccagt ttaagaatac ctttatcatg tgattctaaa
     7561 gtatccagag aatatctgta tgctttgtat acctatggtt atgcataaaa atcccagtga
     7621 taaaagtatt tatcactggg atttttatgc ccttttgggt ttttgaatgg aggaaaatca
     7681 catgaaaatt attaatattg gagttttagc tcatgttgat gcaggaaaaa ctaccttaac
     7741 agaaagctta ttatataaca gtggagcgat tacagaatta ggaagcgtgg acaaaggtac
     7801 aacgaggacg gataatacgc ttttagaacg tcagagagga attacaattc agacaggaat
     7861 aacctctttt cagtgggaaa atacgaaggt gaacatcata gacacgccag gacatatgga
     7921 tttcttagca gaagtatatc gttcattatc agttttagat ggggcaattc tactgatttc
     7981 tgcaaaagat ggcgtacaag cacaaactcg tatattattt catgcactta ggaaaatggg
     8041 gattcccaca atctttttta tcaataagat tgaccaaaat ggaattgatt tatcaacggt
     8101 ttatcaggat attaaagaga aactttctgc cgaaattgta atcaaacaga aggtagaact
     8161 gtatcctaat atgtgtgtga cgaactttac cgaatctgaa caatgggata cggtaataga
     8221 gggaaacgat gaccttttag agaaatatat gtccggtaaa tcattagaag cattggaact
     8281 cgaacaagag gaaagcataa gatttcagaa ttgttctctg ttccctcttt atcatggaag
     8341 tgcaaaaagt aatataggga ttgataacct tatagaagtt attactaata aattttattc
     8401 atcaacacat cgaggtccgt ctgaactttg cggaaatgtt ttcaaaattg aatatacaaa
     8461 aaaaagacaa cgtcttgcat attaagtagt tcagttttgg agtacaaaaa gtgaaaatgt
     8521 aaacctaaat gagtatcaaa ttgcaactga atttgatact cgttttttat tgttaaatca
     8581 acgtttgttg ttaatttaca ccacaaaatg taagttaaaa tgtatataaa aatgcgaacg
     8641 tgaaataatt attgtaggaa tgcaaattgg tgtgttaaaa tggttacaat ttactaagca
     8701 aaaaggggtg cattttccta tgcaatttct tgagccaaaa aataagaacg ctaagtctgt
     8761 ggattgggaa atatcagagc aagtaagggt gatagttaag caatacgctg aatatgcaga
     8821 gcgtactgaa agcgaagcag tggatgaatt cttattgaac attttagacg ataagaagtt
     8881 tattgaatgg attgccaaca agcgaagtaa taagcggatt gttgaaaaaa tgggtatcaa
     8941 agatagagtg ggttgataga caatgaccaa gcttaaaaga ttagccacga aggaagatga
     9001 aattgtagat gttaaaattc cgatttcaaa cgaagaatta aaagataggg ctaaacaata
     9061 tgatttatta actccaaaac gatttgcaac aaggtataac aaaatgttgt tcttgccaac
     9121 gtcttttaaa tggaacggtt ctgaatatcc cattcaatat aattactgca tcaatccgtt
     9181 ttgttgtaat tttgggaaag aacagcacaa attcaaagat gtaaaaggga aaccaagtcg
     9241 atataaaatg actggtagtt ccaaagataa aggtcataaa ggtatgtact gcaatgacaa
     9301 tcctattggc agaggtgtat cgcaaaactg tacagtaacc cctttatcta attggtctgt
     9361 tgttgaagaa attaaacgcc ttatagaaat caatagcatc caagatgttg agccagatta
     9421 tcaatttcat aaagaaggct gctcggaaga agaatcaacc ccattcaatg agccgaagca
     9481 attttacaaa agggggaaaa gtaggggcaa atctcaacgc tatcaatgta aggcgtgtaa
     9541 gaagttcact aatgttctac caaaaagaga agaaaccact acttatcacc aacaaaagaa
     9601 caccattctt ccaatgttcg ctaagatggt tgttggaagg gtttcagtca gtcgtacttg
     9661 tgatatttta ggaattggag ttggaaccta ctatcataag ctggagtggc tgtataggcg
     9721 ttgtttggag tttttagaac gatatgagac acaacccctt caaacaaaga aatttaacga
     9781 gatgtggctt aatacggaca agatgcacta ctacctaaat aacgtccgta agaaaggaca
     9841 aggttcaaag aaatatacgg ggtttgaaga ccttaatatg caaacgtata tagttgtatc
     9901 tgctgaggtc ttatcaagat atgtgttccg ttctgatgtt gcctatgatt ggaatatttc
     9961 aatggatgaa ttaaatgaag atacaagaaa attcaaagaa gaccatctca atacttttag
    10021 tcgtaaaaat gataggttag attggtctta ttatccacaa gaaccatccg ctaacgatag
    10081 cgaaaatagg aatgcttatc ttcacgaatt agggaaaatt actaatagga gtagatttgt
    10141 cgatggttta aatgttgatg ctccttatac tacaactgct cactattggt taattaaaca
    10201 aatggttaat gccgacgaat ggcgaatgat tagtgatgat gacttctcta ttagaaacgc
    10261 cttttataga gtattcacga aagaattaag gttgagcgat gcacatcatt ttatttgcca
    10321 agttaacaag acgaaaagca gaaaacaatg cttaaaagaa ttcggacaag caaaagcaga
    10381 actgctcgat tggggagata tcagaggatt caaaacaaag tttctacgga ctttggcatc
    10441 tcattatcta actgaattac taacaagtca tcaattccac gaagaggcta ttagtaaaga
    10501 tggagaaaga tatcgcaaat atgccgataa ccctatcaaa catccattag ccactaagga
    10561 taaaggattt tattcagttg attgtagaac ggatttatct gctctggaac caaacgaaat
    10621 cgcaaagatg ctattaaatg ttaatgacca ttcgaccaat agctttattc aacagataag
    10681 acgttatata tcatctcttg aaagaccatt aacaacagct cgtggtgata agaagagcta
    10741 tatttacgct aacttcaatc cgaagtatgc tcaatttgcg attacaattc taagaactta
    10801 ctacaacttt tgtcgccctt ttaaatcagc ggataaaaag gttttgacac ctgctcaaag
    10861 attagggatt acagataaac aatttgattg gaaagatata atttatttca aatgacaaaa
    10921 agagcttgga gtaatcccaa gctcttgtgc tttatcttta ctgttcttct tcaattaacg
    10981 cacccgttaa ctcaataagt tttttctttc atatacatga aaatagtagt tatacggatt
    11041 tttatcattc tttatccctt tttgggcaaa tacctcattc cattcctcat aattcacttc
    11101 tggaaaaaaa gtatctcctt cgaattcatg atgtattttt gtgatgtaca ttttctcaac
    11161 ataagggaaa aacaaattat aaatctgttc tcctccgaaa ataaaaattt cttcttcgtt
    11221 tttacataac tcaaaaacat cttctattga atgaacaatt tcacaaccat taaaggtaaa
    11281 ccccttatct ctcgtcagaa taatatttct tctgtcaggt aaggctcttc cgattgattc
    11341 aaggttcttc ctacctaata ttatcggatg tccctttgta gtatttttaa catattccca
    11401 gtccttggga atcctccaag gaatgtcatt ctctttgcca atcactctat tcttatccat
    11461 cgcagcaatc aaagaaactt tcatttataa cacagccttt ttaactaatt taccatctaa
    11521 tttcttattt ctatcaaaaa accttttctc attatcctgc ccgttagttg aataagcaat
    11581 tttttctata agttatatta tcataatacc acaaaatcac aaattttcat tcaattatca
    11641 ttttcaattg cattttataa atcattttgt atgtaatttg gtatgtaagt taaaatgaaa
    11701 aaagtagtcc attttgaagt acattttgtc attttgtact ccaaaactga actacttact
    11761 tgcatatata cgcctttata gtggagtact acatttacga gattcggtta gagtatcaga
    11821 aaaagaaaaa ataaaagtta cagaaatgta tacttcaata aatggtgaat tatgtaagat
    11881 tgatagagct tattctggag aaattgttat tttgcaaaat gagtttttga agttaaatag
    11941 tgttcttgga gatacaaaac tattgccaca gagaaaaaag attgaaaatc cgcaccctct
    12001 actacaaaca actgttgaac cgagtaaacc tgaacagaga gaaatgttgc ttgatgccct
    12061 tttggaaatc tcagatagtg atccgcttct acgatattac gtggattcta cgacacatga
    12121 aattatactt tctttcttag ggaaagtaca aatggaagtg attagtgcac tgttgcaaga
    12181 aaagtatcat gtggagatag aactaaaaga gcctacagtc atttatatgg agagaccgtt
    12241 aaaaaatgca gaatatacca ttcacatcga agtgccgcca aatcctttct gggcttccat
    12301 tggtttatct gtatcaccgc ttccgttggg aagtggaatg cagtatgaga gctcggtttc
    12361 tcttggatac ttaaatcaat catttcaaaa tgcagttatg gaagggatac gctatggttg
    12421 cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa atctgtttta agtacggttt
    12481 atactatagc cctgttagta ctccagcaga ttttcggatg cttactccta ttgtactgga
    12541 gcaagccttt agaaaagctg gaacagaatt gttagagcca tatcttagtt ttaaagttta
    12601 tgcaccacag gaatatcttt cacgggcata taacgatgct cccaaatatt gtgcaaatat
    12661 cgtaaatact caactgaaaa ataatgaggt cattattatt ggagaaattc ctgctcgatg
    12721 tattcaagat tatcgcaatg atttaacttt ttttacaaat gggcttagtg tttgtttagc
    12781 agagctaaaa ggatatcagg ttaccactgg cgaacctgtt tgccagaccc gtcgtctaaa
    12841 tagtcggata gataaagtaa gatatatgtt caataaaata acttagtgcg ttttatgttg
    12901 ttatataaat atggtttctt attaaataag atgaaatatt ctttaatata gatttgaatt
    12961 aaagtggaaa ggaggagatt gttattataa actacaagtg gatattgtgt cctatttgtg
    13021 gaaataaaac aagactacga atacgagtgg atactatact taaaaatttc cctttatact
    13081 gccccaaatg taagaacgaa actttaatta atgttcaaaa aatgaatata ataacaatca
    13141 aagagccaga cgccaagacg cagagccgat aatttgagaa atgaaactct catcttatcg
    13201 gctctttttg tttatctgaa ttttactgac tagccttcaa tatttcttgc ttcattgata
    13261 ccttttgcta gtgcttccat taaggatagt tctttgtctg taaagctatc catgtatttt
    13321 tctacctgta accttcgagt gcttttaatc aaattatctg taggtaagaa aaattcatca
    13381 atagatacat ggagtaatga tacaagatca tacagcactt gtgtacttgg gtgttgtcct
    13441 ttgttttcta tatttgttaa ataacgtggg tcaatttcaa tcattgttcc cacttgttca
    13501 cgagtgagac cccgtttcat tcgagcctct ttaatcgcta aaccaacggc tctaaaatcg
    13561 tatttatctt cttttttacg catactaaat cacctctata cattttactg ttcctatata
    13621 accataaaca ggtatagaaa aac
//
LOCUS       DACDSY010000076        13286 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDSY010000076 DACDSY010000000
VERSION     DACDSY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.077"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..104
                     /locus_tag="IWK44_001811"
     CDS             <1..104
                     /locus_tag="IWK44_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP9550315.1"
                     /translation="VASVKKTVIDPIDEKEAMIEENRKKAMRKRVSR"
     gene            complement(166..1977)
                     /locus_tag="IWK44_001812"
     CDS             complement(166..1977)
                     /locus_tag="IWK44_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP9550316.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(1974..2339)
                     /locus_tag="IWK44_001813"
     CDS             complement(1974..2339)
                     /locus_tag="IWK44_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP9550317.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2502..2897)
                     /locus_tag="IWK44_001814"
     CDS             complement(2502..2897)
                     /locus_tag="IWK44_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9550318.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2915..3877)
                     /locus_tag="IWK44_001815"
     CDS             complement(2915..3877)
                     /locus_tag="IWK44_001815"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP9550319.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3877..4089)
                     /locus_tag="IWK44_001816"
     CDS             complement(3877..4089)
                     /locus_tag="IWK44_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP9550320.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4110..4808)
                     /locus_tag="IWK44_001817"
     CDS             complement(4110..4808)
                     /locus_tag="IWK44_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP9550321.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4828..5370)
                     /locus_tag="IWK44_001818"
     CDS             complement(4828..5370)
                     /locus_tag="IWK44_001818"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP9550322.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5502..6506)
                     /locus_tag="IWK44_001819"
     CDS             complement(5502..6506)
                     /locus_tag="IWK44_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9550323.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6503..7492)
                     /locus_tag="IWK44_001820"
     CDS             complement(6503..7492)
                     /locus_tag="IWK44_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9550324.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7489..8295)
                     /locus_tag="IWK44_001821"
     CDS             complement(7489..8295)
                     /locus_tag="IWK44_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9550325.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8461..9417
                     /locus_tag="IWK44_001822"
     CDS             8461..9417
                     /locus_tag="IWK44_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9550326.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9494..10012)
                     /locus_tag="IWK44_001823"
     CDS             complement(9494..10012)
                     /locus_tag="IWK44_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9550327.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10100..10249)
                     /gene="rpmG"
                     /locus_tag="IWK44_001824"
     CDS             complement(10100..10249)
                     /gene="rpmG"
                     /locus_tag="IWK44_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9550328.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10477..10923
                     /locus_tag="IWK44_001825"
     CDS             10477..10923
                     /locus_tag="IWK44_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9550329.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11118..13013)
                     /gene="asnB"
                     /locus_tag="IWK44_001826"
     CDS             complement(11118..13013)
                     /gene="asnB"
                     /locus_tag="IWK44_001826"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP9550330.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4139 a         2821 c         2260 g         4066 t
ORIGIN      
        1 tggttgcttc tgtgaaaaaa acagtgatcg atccaattga cgaaaaagaa gcaatgatcg
       61 aagaaaaccg taaaaaagca atgcgtaaac gcgtttctcg ttaataatta tttaaaaata
      121 cgcatgaaga ctttctaaaa aacagataag tcatcatgcg tatttttatt tgtcagtcaa
      181 tataagttgt gctattttat ctgcctgtct cagattccat ggatctgtat aaggtctagc
      241 tactgctgca gttagccaat ccacaccttc aacaggtatt ccatagcaat aaaagtcaga
      301 ctggatttcc cctgaagcat caatcacgcg attcgtacct cgctcaacat ctatagcttc
      361 tgaatgataa cttcctgtat tatctgaaaa tgaaaaaggg cgaacaagtt tgttttcctt
      421 caaatttttt atgacaggat tacttgaacg agaaaagctt gtttgtggca atcgagcttc
      481 aatcaaatag cgactgctga tttctttatc ttgtagagaa ataacatagc ggttgtgctt
      541 ttgctgaata tcaaatggcg gttcgatcaa atgaaaaata cccgcatcta tcagtgctgc
      601 taattctttc gtccgaatga ttggcggacc gatcgttaaa aagctattta gatgagtaaa
      661 ccatccccat agtatttctt tgtattcctt agcagaaaaa acgtgccatt caatagcctg
      721 ccggattgga tcacgccagt cttttaatgc atcaaatgtt gaagcaaatg ctcctgtgca
      781 attgcctttt tccgcttctt ttatctgccc ttcaataaat attcggctag cttctgaaaa
      841 gggctcattc tgataggcaa gaggagtttc taaatacgac caatcccatc tataagggat
      901 tagtgctgga tgctttccga gacactcctc tgaagagagg gagagaaaat catgctcaaa
      961 atttttcttt ggaatcggca agtgatattc ttcgatgatt ttcttgtaat aaaacaactc
     1021 aacgtctttt ttcagatagt caaaaaatgt ttcgccagtc aaaaatcccg actgatgcca
     1081 ttgctccagg ttttcttttg ttagtaaccg tggccatgcc attgctccgc cttgcttttg
     1141 atttcttcct cgagggtaat aagggagccc ccttcttgat ccactgtata caatcggttc
     1201 cttgcctgat ggatgataga ccagttttcc cttttcgttc tcgaagcgcc ctcctcttga
     1261 tactgtaaac aatccgacat aatcaaaaaa acttaagcct agacctctta aaaagacatt
     1321 ttctttaggc ggtatcttcg aaagatctac atctgcagga ttagaaggaa gctgatacag
     1381 taaaacattt ttctttgcgt agtctaccca cactctttct tcttctgtct catcattttc
     1441 ccagtgaccg cttgctaaaa caagctgatc gatagaatag ctttgctgat ctgtctgtaa
     1501 aagataacca tcttttcttt tgcttacttt ttttactaaa gaataaacaa actgaataga
     1561 agctggaaat tcttcttgca actttgagaa aaaccagcgt tgatagagtc cgtataaaca
     1621 tcgagtagat tgctgatctt tttccagacg attgatttct tctataatta gttgcttatt
     1681 tttggttacc gtctgttcga tataatctgt agcaggtcct ttactccatt gaaacaagtt
     1741 gggtccctgt gttaccaatc ctccactgga caatgtttca tctgtaaaaa gcgtcacttg
     1801 ttgagaaaca gaattcatta ataactccgg tggctgattc aaccgccaaa ctcgacctcc
     1861 aggaccattg ggatcgaaaa gaaaaagttc aagagaatgt ctgtcctgat tattatggag
     1921 aatcctttct gctacactta atcctctagg tccggcacca atgatcccta ttttcatttg
     1981 catgcctcca tttcgataaa ttttttcaat aaaacagcat ggttctgatg ttcatttttt
     2041 gcaccataaa caaaagtaat gactggatgt tctttcataa gttgactgag tttaaaaata
     2101 gcttgcttct tttcttcttc gaaaatcaat tcttgctggt atttctttgc aaatgaagca
     2161 aatcgttctg gtacatggtc aaaagttttg cgtgcttcat tactaggtgc aatttctttc
     2221 aaccacagat ctaactgcgc tttttctttt gaaattcctc gtggccagaa tctatccacg
     2281 agtattctat agccatcctc tttttcagca tcaaggtaaa cacgttttaa tttgatcata
     2341 gttgtttcct tctttctacc ttttcgctta gcttatatat gtagataata gcacaaaata
     2401 aaaaaaatga acggcacaac tatccattgc gccgttcatg tgatcttggt caaaatctaa
     2461 tgattgattc ggtttttaaa gctgtcatac aatcatctgt tttatacttt tttacttttg
     2521 atttttccag accatccgtc atatccaccc ttaagtaaat aaatctcttg ataacctgct
     2581 ttacgtaatt tatttgctgt acggatgctc aaacttttct tttgatcgta aatatagaca
     2641 ggctggtctt ttcgaataga tcctaatgaa tctttcaaaa cagtgtaagg gatgtttctt
     2701 gcacctaaaa tatgcccagc atcaaacaca tctttttcgc gaacatcgat tacttgtgct
     2761 tttctcattc cttctcgaaa ttcttcttct gtcaaagtcg ttgctgaacg tttggccatt
     2821 actcgtagat agacttccca aaggatgatt gcaagcacaa tcaatagcaa aatgatatta
     2881 attacccata aaaccatgta atgcctccgt ttttttaagc aaatttcaag gctaatgagg
     2941 cagcaccaat gacaccagct tcattaccaa gctctgctaa tttgattttt gttgattctc
     3001 ttacttgcgg gaaagtaaat tctttaaaat atttctctac acggctacgt aaaaattctc
     3061 ctgctgccga tactcctcca cctaacacga tacttgacgg atttaatgta ttccccaagt
     3121 ttccgcaagc taaacctaag tagaagcata cgcgatcaac aaccatcaat gcaaacggat
     3181 cgtcttcttg agccatttca aagatatctt tacttgttac ttcttctccg ttgtctagca
     3241 ttgctttcag ttttgaatcc cctgcgtatt cttctgccaa gaaacgacct aggcgtacca
     3301 cacctgtagc gctagataca gtttctaaac atccgcgttt accgcaagta cattcgaacc
     3361 cttcaggatc cacagtgata tgtccgattt ctcctccagc tccgctcacg ccgtgaatca
     3421 aatttccgcc agcaacgata cctccgccaa cacctgttcc tagagtaacg aatacaacgt
     3481 ctggatcgtt ttctccagca cctttccatc gttcacctaa agcagcaaca ttcgcgtcat
     3541 tatcgatagc gaatttgatt cctgttcctg cctcgatcag ttttttcaca aattgaactt
     3601 ctgtccagtt taagttatac gcaccaataa ccgttccagc ttcgctgtcc acgcttcctg
     3661 gggtacccat gccgattccg ataaagtctt cggctttcat attatataaa ttcaaacgat
     3721 gattgattga ttcaatgatt tcaggaacga tatgcttacc atcatccaaa atattggtat
     3781 cgatgctcca tttttgctga atctctcctt ctggtgttaa aattgcaaat tttgctgttg
     3841 ttccacctaa atcaatccca ataatcttct tgtccattac aaatttcctt tctttcggct
     3901 ttcttcaatt cggtgctctc tttttaatat gcgccacgct gtcatgtatg tatcatgatc
     3961 aatcaaacgg ctatcataca gattacgcaa ttcgatagtc attaattcaa tgtcatagat
     4021 gcgtgcaccc acatagataa agatgccaaa ttttttgaac agctgctgta catcatataa
     4081 gctctccatc tttaagcact tccttcatat tatacaagta aaccatattt ttttaaaccc
     4141 agtaaaatac atattacaaa aagaaataca aaaactagtg tagaaagaat cctttggtgt
     4201 atcgaatagc ttgtctgttg tttcggtaga cccaaaacat tccctaacac taatccgcca
     4261 attagcccac cgacatgtcc ccaaacatct acagaggtat ccagaagacc aaaaacaaat
     4321 gtcaacacaa taaataaaag aaaatgtctg actagctgct ggatcgctgg gttatgttta
     4381 aaatgaaacc ctaagataaa caacgcaccg aacaaaccga atatcgaagt acttgcacca
     4441 ccagacagaa cattcgcttc gttaaaagca aagcttgctg tatttcccat tatcccgctg
     4501 aacatataaa tcaagaaaaa acgccaatgg ccatagattg cctcgatttg ctgccccata
     4561 aaatacagta tcagtgagtt cattacgaaa tgcatcagtc caaaatggat gaagattggt
     4621 gtcactaaac gccaccattc attgaagtga acaagataag gaccatacat tccggcataa
     4681 taagggatct caagtcgcgg aaataattcc atcatgagga agaccgctgt ctgtatccct
     4741 aaaaaagcat aggtccataa cggtcgtctt aaccatgcac gcaattttat ttgctgttga
     4801 taattcatga aaacctccca tttcatttta gtcaataaac aattgttgga tcttctgatc
     4861 aaagctctcc ggttgccatt gttcattcaa ttgttcacga aaaacaaggc tacatgtttt
     4921 tttatgaaaa ttatttaaat accggtcgta ataaccaccg ccaaaaccga ttcgataacc
     4981 ggctctattg tagataatac ctggtacaat gaggagatcg atcatctccg gcaaatagag
     5041 cttctctgat tgtggctcaa gaacaccaaa actcgaacgt acaagaacgg actgttcatc
     5101 catttcataa aacgccatct gacgtttcgg caaagtttta ggtacaacta ttttcttttg
     5161 ttctttttct gctcgttgga taaccggctg tgtatccagt tcaaattcat tcgataaagt
     5221 cgtccctatc acttttgctg acttccagta ctcgctggcg aatagctgtt tatagattcg
     5281 ctgttctttt tcctttttct tgccactttt ggccagattt ttcagctttt cgattccttt
     5341 ccggcgtaat cttactttca attggtccaa aaactcaccc ttccttctct cataaaatgg
     5401 tataggaaaa aaagaaaaat agcaatcgaa ctttcaaata aatgcataaa tagaataaaa
     5461 agaagaccta gaaatgattg gccttccttt tttaatgaat ttcaactgtt cagcgcgtgt
     5521 ttttgtctga taaaaagtgc atacagaaaa tatggcgcac caaaaagggc aatcaaaatt
     5581 cctgtaggta cttccccact tggtagaatc actctagcaa ccatatctgc actaagcaga
     5641 aaaatggaac cgaacaaacc gctcagaaca aaggtccagt tattttcttt acgtacgact
     5701 agcttcgcag catgaggagc tagtagtcct acaaaagata aactacctgt caccgctaca
     5761 ctaccgcttg ccaaagcaac agctaacaac aaaaggattt tttgactttt agccacgtta
     5821 aggcctaatc cttgcgcgcg ttcttgacca aatgatagca atacaatcct ctgtgcataa
     5881 agtcctccta gcggaattaa gataaccgcc catggaagca atgcccaaac ataaggccag
     5941 ctcgcacccc agattgatcc cgctaaccat gcattcacaa atccataatt ttccttagat
     6001 aattggatcg tgcatagcaa tgttaatgca gaaaggccgg cattaacggc aataccggac
     6061 aataagattc gattcatacc caaaaagcca tttcgatcat aggccgcaag atagacacag
     6121 caagcagcta aaacaccacc tagaaaggct acaactagca atagaaacgg cagcgaatag
     6181 tgcgaaaaga aacctaaata aagcatcact aaaaatcctg ctccggcatt aatgcccaat
     6241 atactagaat ctgccaattc atttcttgtt actccttgca agatataacc agaaacgcca
     6301 agacaagctc ctgctaataa actgatcagt aatcgaggaa ctcgaaattc tctaacaatc
     6361 agcgccaaag aagatgattc tttccctatg aacgtggcaa aaagctgatc caggtaaata
     6421 aaaggcgtac caatcattaa actacctata gacaggatga caagtacaaa aagcaagaaa
     6481 tagaacgtcc aatatatttt tttcaccgtt gttccctcct tacttgtatc agtaaaaacg
     6541 gaacgccaat caatgcaatg actaatccaa aaggtgtttc aaaaggggga ttgatcatcc
     6601 tcgctgtaaa atccgccaac ataaccaatc caccgccaac taaaaaagag acaggtagaa
     6661 cataacgata gtccgttcca accattccgc gaacaactgg cggaaccaac agcccaataa
     6721 atgaaacagg tccgactaac gaaacggcaa tggctgctaa cactgcaact agacagaaaa
     6781 caatcaaccg cgttcgttta ggatgacttc caagagctaa tgcatgtgtt tcactcaaat
     6841 gaatcaaaga tattgctttt cctaatgaaa aagaaaccaa aacagcaagt atatataaag
     6901 gaaacaatac tgctaattgt ttccatgtga tattggcagc tccgcctaca aaccaaaaag
     6961 caagatcctg ctgcagatta aagaattggg taagtgcttg gctgatagca gtaaaaaaag
     7021 aactaatagc tacgccagct aaaatcagct gtaaaggatt catgcccatt ttcgaaaact
     7081 gcaaaaaacc aaatacgagc aaagttacag caaaagaacc tgccaatgac attagaaaca
     7141 cagaaaaatc agaagcttta ggccaaaaag caaaactaag ggccattgcc aacgaagcac
     7201 ccgcgttgat tcctagtaag cctgaatctg ctaaaggatt tcttgtcacc ccttgcatca
     7261 atgcaccgct caatgcaaaa caactcccta caagaaatgc tcccgccaca cgaggcaaac
     7321 gaatatttcg tatgatttgg tgactttgtt ttgatctatc aaaatcaaac aacgcctgcc
     7381 aaaaatctgc tttttccaca gtcgctgcgc catttgaaag agcaacaaaa aaaataatca
     7441 aaagaacaaa acagaccaaa agcaggagtc tatttttctt catagtcctc ataattgatc
     7501 ctcctgctct tttggcaaca aatcataggt tagaaaaact ggtttatttg ttcttggaag
     7561 cgtgacaatc gttgcatcaa tctcaaatat ttttttcata cactcttttg tgatcacttc
     7621 ttcaggtgta ccttctgtga tcaacttacc gtttttcatt gctaataaat aatctgaaaa
     7681 acgtgaagca tgattaatgt catgaatcga catcaagatc gttgtttgtt tcatttgatt
     7741 gatttcagtc aataactgta ggatctctaa ttggtgtgcc ggatctaaaa atgttgtcgg
     7801 ttcatccaag atcaagatat ctgtttcctg agcaagcgcc atagcgatcc agacacgctg
     7861 ggtttgtcca ccagacagcg ttgcgatatc ccgcatacga aatccagcta gacctgtagc
     7921 atccaacgcc cactcaattg cttcatgatc ttgcgtgtcc atgccactga acagtttttg
     7981 ataagggtat cgtccgtaag atactacttc ttctactgtc atacctgtag ggtgttcact
     8041 agtttgtgct aataatgcaa ttttttttgc aagttctttt gaactgtatg tagagatatc
     8101 agagttattg acaatgatct ttcctgattt cacaggtagt atacgggtaa aacttttcaa
     8161 taaagtagat tttccacttc catttggccc aatcaaacta gtgattttcc cttcaggaat
     8221 cgtaacagac atgttttcaa tgattttttt gttttcgtag ccggctgcaa tgtcagctgc
     8281 tcttaatctc gtcatttcac ccatcctttc gctgaaaatc attctcaatt atgacgctgt
     8341 ttcaacattt cgtcaagcgc tactttcata tctatagcca aatgaagcgg ttgacagcgt
     8401 tcgaaatatc aggaataata accaatgaga aagattctca attatgttag gaggaaagaa
     8461 atgaaattaa aaacaaaagt attcacatta tctagcatat tgctgctggc tggagtactg
     8521 agcgcttgct ccccctcttc agataaagaa acagctacaa ctgccaattc gacgacttca
     8581 tccaatagta ctcatacagt cacagataca cttggacata aagtagagat tccaaacgcg
     8641 cccaaacgga tcatcggcag ttacttagaa gactatctga ttgcactagg ggaaaaacct
     8701 atcgcccagt ggactgttgg cagtggttcc attcaagatt atctgcaaga tgatttaaag
     8761 gatgtcccaa cgatttctta tgaccttcct tatgagaaag tgttaagctt tgagcctgat
     8821 ttgctgttga tttcttcttc cgctactgtc gaaggcggga aatacgaaca atatagcaaa
     8881 atcgcgccaa cttatgttgt aaaaaacgga acagatgtca catgggaaga acaactaaaa
     8941 gatgtcggca aagctttggc aaaagaaaaa gaggcagaaa aaatcatttc cgattatcaa
     9001 aaatccatca agaaaactcg agaagaacta gcagataaaa tcgagaacaa gacagctgca
     9061 gtgttgtggg tgaccaacaa ctcagccttt atggtcagca aaaatcggtc aagtggaaga
     9121 atcgtatacg acgatctgga atttggtgtt cctgatctag tggaagaggt ctccaaaaaa
     9181 gccacttctg actggtctgc tgtttcctct gaaaagctag ctgaattaga tgctgactac
     9241 atcatccttg tgaacagtga caaagatgct gccatgttca atgaaccaag ctggaagaat
     9301 ctaaaagcag tgacagacaa tcatgtactg caattcggac ctgaatctag ttggctatat
     9361 aacggaccta ttgccagtca ggaaatggtc aatgatatca aagaaaaact aaattgattt
     9421 taactaaaaa aagcatcttc acgattagtc gtgagatgct ttttttctta ttaacaaact
     9481 agacgcatag attttattct tcttttgaag caaccctatt ttttactctt tcccattttt
     9541 gaaaacgatg ggcatataga ttttcttgat ccgttatccc ctcatactcc tctataaggg
     9601 cgaagtctga aaaatcaata ttcccaatat acgtatcgcc ttcaaacgtg ctatcgatca
     9661 atgttcgcca gatccgacca gtgtaaggaa gaagttcttg aaaaatccga ctaccacccg
     9721 aaacatagat atcttctgag atcgtttgac tatacgacaa aagttcatcg atcgaatgca
     9781 ttacttccgc attttctttc gtgcgaaaat ttggttgcgt tgtcaacacg atgatatggc
     9841 gataaggaag tgaaagatcc cccattcctt cataggtctt tcttcccatc actaaaattc
     9901 gatccattgt atgttcgcga aagaaacgca tatcatttgg caatcgccat ggtaacaaac
     9961 catttttgcc gatcagacca tttttatctt gtgcccacat ggaaataaac atattatccg
    10021 ctccttatga ttttagatgt tttgagataa aaaaagtctc aattctttga ccagcaagga
    10081 attgagaact ttaaaaacat tattttgttt cacggtgcaa agtaactttg cgttcgcgtg
    10141 ggcaatattt ttgtttttcc aaacgatcag gattgttacg tttgtttttg cttgttagat
    10201 agttacgttc tttacaagaa gtacactcta aagtgatgtt tacgcgcatg tttttccctc
    10261 ccatttttac aagaatcctc taagatgact ctcagacttg attatcttac catgattttt
    10321 tctagattgc tagtgttttt cacgattttg ttaagtaaaa ctacttgacg ataaatacaa
    10381 cggttttact tctcaaacaa aatctttttc tttttgtata aataccctta cacttgttta
    10441 tatagattat gttttttaat aaagggggat actaatatgc catggaacat gaaggacttt
    10501 cctgcatcaa tgaaaaactt agataaattg acaagaaaaa aagcaatcga catcgcaaat
    10561 gcattattag atgaagggta ccctgataac cgtgcgatcc caattgccat tgatcaagca
    10621 aaaaaatggt atgaagatgc tagtgaatca gaaaaacgaa ctttcgaaaa agaaaaaaat
    10681 ccaagcaaga cggatgaaca cgatacgaat cctcgtgctg gaaaactgct tgattcagat
    10741 gtgatcgtag aatatgagga agaacaatgg atcgtgaaat ctaaaggggc aaaaaaggca
    10801 agcaaccatt ttgatacaaa aaaagaagca atcgaaaaag gaaagcaagt agcacaaaac
    10861 aaagaatcaa ctttgttcat ttataaaaaa gatgggacaa aagaaaaaga aatcgattat
    10921 tgaacatatg taaaggggtt gtgacgaaaa tcgtgtcaca acccccttat gttcgaaaca
    10981 atggtgttcc tctgccaact ccgaaccata agtcataata tctataaaac gggaagagct
    11041 gttttttgaa attgtttctt atcactcagt gttaaacggt aggagaaaac ctctttttta
    11101 ttgatatttg attttcatca aacagtcaca tagtcgattg cttctgcttg cggaatcgtt
    11161 tcatctacaa agtagacttt ataccatgtc aagaaactga agattgtcca gatttttctt
    11221 tgctcttctt tacgtccttc gtgatggtca tctagcaact tcatgatttt ttcttgatca
    11281 aaaaattctt ttgcaaaatc tgcttcgaat aatgctttga cttgttcgta gccgtgttct
    11341 tctttcaacc aagctttgat tggcacaggg aatcctaatt tcacacgatt cgaccattct
    11401 tccggaagat gtttgttcgc agcttgtctg aaaatatctt ttgtgttgtt ttgattcaat
    11461 aaaaacttac ttggtatttg ttgagccaat ttcatgactt cgatatccaa taaaggtact
    11521 cggacttcca aagaacttgc catagaaagt ttatctgcct ttaacaagat atcttttggc
    11581 atccattgat gaagatcgac atattgcatc ttattgactt catctttgat tccttctgtt
    11641 ttctcgtaat gaacagtcat gatttcatcc acagatggtg ctgtttgata ttttggtgtt
    11701 aacacttctg atgcttctga ttcttcgaac actcgagcat gaccgataaa atattctttt
    11761 gcaggagcaa gtgattcata taaatggatc cgtccatgga agtttttcat ttttccgatt
    11821 ttacgtccta aattatagcg tgttcctttt ggtaattttt tcaatccttg agcaatcaca
    11881 cggatgaatt ttgaattcgt atggaatcca tacgtttggt atcccgcaaa cagttcatcc
    11941 gccccttctc cagattggac tacccgcaca ctttgtgaag caagatttgc taaaaagtac
    12001 aaaggtacgc aagaaggatt tgaatcaggt tcatctaagt gatactggat caaagggaat
    12061 gctttgaacg acatgtctcc gtcgatcact gctgctgtat tatctaagtc caatagttcg
    12121 gttagttttc tggcttcaat cgcttcatta taggttttat catcaaaccc gattgagaaa
    12181 gaatgatctg gtttcaatac agaggttaca taactggaat caaccccgct ggataagaaa
    12241 gaaccaactt ctacatcact gaccgtatgt gcttcaatag aagcttgaac ggtttcgtcg
    12301 attttttcga tccattcttg tcgggaatgc gtttcttttg tttcaaaatc tgcatcccaa
    12361 tattgctgaa tgtctatttt tccatcttta tacgtataat aatggccttc aggcaaacga
    12421 tagacatctt tgaagaatgt ttcgccgttt aatggagaat attggaaagt catataaggt
    12481 ttcaacgctt ctttgttcaa ttctttgtta aaatcaggat gaggcagaaa acttttgatt
    12541 tctgaaccaa acataaatgt tccgttcatt tctgcataat aatatggttt gatcccaaag
    12601 tggtctcttg caccgaataa ctcattcttt tcattatccc aaatagcaaa agcaaacatt
    12661 ccgcggatct tttgcaataa ttctgttccc cattcttcgt aaccatgcaa aaggacttct
    12721 gtatctgcat gtgtttgaaa aacgtggcca gctgcaatca attcttctct caaaggctgg
    12781 taattataga tttcgccatt aaaaataatg atctttgtcc gatcttcatt gtaaataggc
    12841 tgagtaccgc cctctaagtc aataatactt aatcttctga atcctaaagc aacatgttta
    12901 tcgatatatt ctcctgaact attaggtcca cggtgaacga ttcgatccat cattgtatta
    12961 ataattgttt ttttgttgtc cttgtcgtta acaaaaccta caataccgca catttaactt
    13021 tcacatccta atataaaatt actgtttatt tcattcatat gcatcttatc atagaataaa
    13081 tagcggacga atattttctc tcttacaatt tcgtcacgtg aaaacaaaag atgaaagttt
    13141 ctgtttttac agaaaataaa gtcccaatat ctaatttacc atgttttctt ttttgaggct
    13201 accttattct cttcgtgtta aaaaaatatt agtggatgct atttccaaaa aaaccagcaa
    13261 aaaaagacac gacctaaaga tcgtgt
//
LOCUS       DACDSY010000077        13146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDSY010000077 DACDSY010000000
VERSION     DACDSY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.078"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            216..1118
                     /locus_tag="IWK44_001827"
     CDS             216..1118
                     /locus_tag="IWK44_001827"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP9550331.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            1331..3106
                     /locus_tag="IWK44_001828"
     CDS             1331..3106
                     /locus_tag="IWK44_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP9550332.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(3172..4758)
                     /locus_tag="IWK44_001829"
     CDS             complement(3172..4758)
                     /locus_tag="IWK44_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP9550333.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            5009..5233
                     /locus_tag="IWK44_001830"
     CDS             5009..5233
                     /locus_tag="IWK44_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP9550334.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            5375..7126
                     /locus_tag="IWK44_001831"
     CDS             5375..7126
                     /locus_tag="IWK44_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9550335.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            7126..8910
                     /locus_tag="IWK44_001832"
     CDS             7126..8910
                     /locus_tag="IWK44_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9550336.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            9236..10087
                     /locus_tag="IWK44_001833"
     CDS             9236..10087
                     /locus_tag="IWK44_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9550337.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            10141..11892
                     /locus_tag="IWK44_001834"
     CDS             10141..11892
                     /locus_tag="IWK44_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9550338.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            11896..12066
                     /locus_tag="IWK44_001835"
     CDS             11896..12066
                     /locus_tag="IWK44_001835"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550339.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(12106..13008)
                     /locus_tag="IWK44_001836"
     CDS             complement(12106..13008)
                     /locus_tag="IWK44_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9550340.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT         4207 a         2330 c         2722 g         3887 t
ORIGIN      
        1 gacaaggctg tttttggcag gaatttcttt tttaaaagag ttaaaacgtg agaaaatgat
       61 ctttattata aaagagttct taagcttttc tttagagaaa atttcagtta ctatggagaa
      121 cagggcaaag aaaaatcaat cattttcttt gtatatcatc ttaccaatat ataggaattg
      181 ttagtaacaa ttgcctttca aggaggaatc catccatgtt attaggctca cacgtcagca
      241 tgagcggaaa gaaaatgctg ttaggagcag cagaagaagc agccagttat ggatcttcga
      301 cttttatgat ctatacaggc gcccctcaaa acactagaag aaaagccatc gaagagatga
      361 atattgatgc cggaaaagcg tacatggcag aaaaaggtct aacaaatatc gtcgttcatg
      421 ccccttatat cattaatttg gggaatacga tcaaaccaga aatgtttcct tttgccgtcc
      481 aatttctgag agaagagatt gttcgtgcgg aagctttagg cgctactcaa attacacttc
      541 acccaggtgc tcatgtaggg gcaggaacag aagccggaat tgctagaatt attgaaggct
      601 tgaatgaagt tttgacaaaa gaacagacgg cacaaatcgc tttggagaca atggcaggta
      661 aaggtacgga aatcggtcga acatttgagg aattagcgca aatcatcgat ggagtaaaat
      721 tgaatgaaaa attatccgtt acttttgaca cctgtcatac gaatgatgcc ggttataata
      781 ttaaagaaga cttttcaggt gtaatggaag agtttgataa gatcatcggg ttagatcggc
      841 ttaaagtact ccatatcaat gattctaaaa atccgcaagg ttcgcataaa gaccggcacg
      901 ccaatattgg ttttggaaca atcggattcg acacgctgaa taagatcgtc catgatccaa
      961 tgtttgacaa tatttcgaaa atcttggaaa ctccctatgt aggcaaagat aaaaagaaag
     1021 cttacgcacc atatggaaaa gaaatcgcca tgttcaaagc gcaaacattc gatccagaag
     1081 tgtttcctga gttattagca gaagaagtga tactctaaga ttttctatct gacagcagca
     1141 aaaaaggacc aggcacattg ccttgtcctt ttttgtttaa gaaacaaagg agtacttttt
     1201 gcagtctttt tcatcatgca tcttctcccc cctcttttta aatttagaca aacaaggtat
     1261 actggtagaa aagtaaaaaa acgaaaagtt tcacaagcaa gtggcaaaac gtgattggaa
     1321 ggacgaaaag atgctgaaca ctttaaaacc cttgatcaac caactaaaaa agaaacatac
     1381 atactttatt cttggcatag ccctattatt tacaattatt cgtttgtatt tatatagtaa
     1441 agcaatctat ggaacagaca tgtcggcaac ctatgatgac cagctgctga ttcgctatgc
     1501 tcaaaatatt ttggacggta aatggctagg ggagtatacg actacaacac tttccaaagg
     1561 aatctcctac agcttatttt tagtacttgc acataaactg catctttctt atgcagtttt
     1621 gttcggcatg ttcagtattt ttgccagtat attggttgca ctcgcgttta aaccgattgt
     1681 gaagaacaag tatcttcttt taagtatcta tttttatttt ttgttttctc cgattagttt
     1741 tacacatgaa tattccactc gaacatatcg taatgccctc gtgattccgg ctgttgagat
     1801 cattatcgct tgtttattag ctatttatta ccgcaaatac ggttctagca gaaaattgct
     1861 gccatggttt ttagggcttt ctgtcgcttt tcctttcttt tggttcattc gcgaagattc
     1921 tatctggctt cttcctttta ctgtagtagc tttgggtatc agtttcgtcc agatcttgct
     1981 agggcaagac ttttctttca aagaagtaaa gaagttaaaa aaacgactct ttcttctctc
     2041 gaaacgggaa tggctgaaac tttcattgtt tttacttcct atagttggct tttccatcgt
     2101 ctatcaatgg atcgaatata aaaatgagac aacatacggt attgcaacca tcaatgaccg
     2161 ttccagtgga gaatttggac gtttgatgaa acaactgatc cgaatagatg atgaaacgaa
     2221 cttgaataaa gaaaacagtg agatatgggt ctcacatgct gcactaaata aagcattagc
     2281 cgcatctcct acattccata ctgtttctac ccgtattgac gaattatata cgactcacgc
     2341 gtggactcaa ggtggaaaag taaaagaatt acccggagat atcatctttt gggcattgcg
     2401 agatgtggta agcgaatctg gttattacca aaacaatgca aaagaaacca atgatttttg
     2461 gaaaaaagtc aaccaagaat tagaagaagc ctatcacaag ggaacaatca aaaagaaaaa
     2521 agagttctat ctgatgggat caggagatgg aaagacgatg agtgatctgc caatcgtatt
     2581 cgatttcctt caatcaggat acgcttatgc atggcgatat aaagatttcc atcaaggagg
     2641 aaattatagt ttcggaacgc aagaacaagt caaacaggct caagaattat taaaccttcc
     2701 gctaatggag aattggatcg atagtaatga accgagaaca cctaatttcc aattaacaaa
     2761 atcagcaaaa gtcgcgaatg tcatcataaa aatttaccaa gttctggcta ttcccgtgct
     2821 gattgcttca atagcaggga tattacttat gatactagga accgtatttg caaaagctga
     2881 tcgtgcctat tatagttcct ctttgattgt ggtcaccgga ttaatactga cacaattggt
     2941 ttttcttttc ggtgtctcat ggttttgttc ttatgcgcca gaaaaaaagg actatttttt
     3001 aagtgtctac actggtgcag gcgtgccatt aacgcaattc atcgtagtaa caagcatgct
     3061 agttttcagt aaattaaatc tgaaaaaagc tttatcttct aaataaaaag acccttggta
     3121 gctagatttt taacctaact atcaagggtc tttgtgtgga aaaatcagtt tttattcgat
     3181 ttcttctacc gtttcctgca aaagctgttc atattttttg tttcctttat ataaatcacc
     3241 aatcgtccat gccaacagga gaaggacaag aacaatgatt acataagcga tgtgatcagc
     3301 caatgctagt tgagcggaag tggcttgctc gccgaagaaa ctttcgatct gtcctggtaa
     3361 tcctcgtaag ttcaagtaag ttagactaat cacagagaac cagccaagaa gttttacgat
     3421 gaatgaattt ttaaatcggc tacccatctc cgcttggctg ttggtcatca tcagtaaagg
     3481 aatcatcgaa aacggcaacg caaaagccaa gaacacctgg gattcattca ttaagttatt
     3541 caaagcggca tgttcttcga tcgttccttt tgtacttgta aacaaaacac aaatcaatac
     3601 aggaatcaca gaaagcaatc gtgtgaccag tcttcttagc caaatcggga tacgcatatg
     3661 gatgaatcct tccatgatga cttgaccggt caacgtacca gtaatagtag agttctggcc
     3721 agaagcaaga agtgctacag caaataaagt ggacaagata ccagattttg ctacagcaat
     3781 caaaatacca ttgctcatag ctgatggatc agataaagct tcatacagac caaaaaagga
     3841 aggatcttgg acagaaccgg ttttgaagac agcgacaccc atgatcaata gcagtgcatt
     3901 gactagaaaa gccattgaca actggatatt agaatcccaa gtagaaaaac gtacagcttg
     3961 tgctacatct tcttcgtttt ggtgatcgat cttccgagtt tgtgaaactg ctgaatgaag
     4021 atataagtta tgcggcatga ctgttgcacc aataatccct aaagctccag tcagcggagt
     4081 ttcgccgccg atgaccggtg agtctgagaa tgtatgactg ttagggataa agccagcaaa
     4141 taaactgtgc caatctgggt tagacaagcc aacttgatag acaaaaacga ataaaatgac
     4201 gaaaattaag cagacaacca gtgcttcgat cttacgaaac ccgacttttg tcagtagtaa
     4261 gagaacgaaa acatctaata cagtaataaa gacggcaatc accaatggaa tatcaaaaag
     4321 taagtagagg gcgattgctg caccaatgac ttctgcaata tctgttgcca taatggctaa
     4381 ttcagtcaag atccataaaa tgacacctaa tgtcttactt gtgcgtgctc ggattgcctg
     4441 tgccaaatcc atttggctca cgattcctaa ttttgctgcc atatactgta aaagcatagc
     4501 aatcagacta gacatcaaaa taactgacat caataaatat tggaaatttt gtcctccggt
     4561 gatcgaagta gaccagttac ccgggtccat atacccaact gctactaacg caccaggtcc
     4621 agagtaagca agaagtgttt taaaaaaact tttccccttt ggtacatcaa ctgttccatt
     4681 tacttcagct aaagataaac catttgtatg ttcaataagt cgatgcttat gaaattgttt
     4741 attggattga ttattcaaga aaaaaccacc tttcctattt atctacgatt acattctaca
     4801 cctcaaaaag aaaaaattaa agaaaaagat tcggtacgcc taattttttt tcatggcgcg
     4861 tcttgctaga tgacgaaaga aattttgttc tcctctaaaa aaaccactat tttttgcatg
     4921 aaactaaaga atacgccttt cttttttcaa taaactttag taaaataagt cattgagagc
     4981 aattcggaaa tagaaaggag agatttatat gttatcaaca ggtattgtcg tacttattgc
     5041 tattattgct ttgctaatcg gcgcagtagg tggctttttc ctagcgcgta aatatatgaa
     5101 agactattta gaaaaaaatc ctcccgttaa tgaggacatg ttgcgatcaa tgatgatgtc
     5161 aatggggcaa aaaccatctg agaagaaaat tcgccagatg atgcagcaaa tgaagaacca
     5221 agggaaaaaa taggttctga cccaacgact ttttacgggc tgattttttc agtccgtttt
     5281 ttatttcgta atttgttaga ctattcagat tattcagcaa aaaagacaag cacagacaat
     5341 cctaggctct tgataaagac aaaggaggag taaaatgtcg atttttagaa aattaggctg
     5401 gttttttcgt caggaaaaga aacattattt aattggggtt ttctcgctat tcgcagtggc
     5461 cttggtacag ttagttcctc caaaagtgat tgggatcatc attgatgaga tcgccgataa
     5521 aaatttatca ataaagatca ttttcgggtg gatcttccta ttgattatcg ctgctttttt
     5581 acaatatctt tttcgttata tctggcggac gaatatttgg ggaagtgcag ctcgattgga
     5641 gaaagaactt cggcagcagt tattcaaaca ttttacagaa atggacaatc tcttttacca
     5701 aaaatatcgg actggggatt tgatggctca cgcaacgaat gatttgaatg cgatccagaa
     5761 tgtagcagga gctggtatct tgacctttgc agattcagtg attacgggcg gaacaacgat
     5821 cattgcgatg attttgttta ttgattggcg cttaacactg attgcgctga ttcctctgcc
     5881 tttgctggcc gtcacttcac gagtgctggg gtcaaaactc catgatgctt ttcgtgattc
     5941 gcaagaagct ttttctagta tcaatgataa gacacaagaa agtatcactg ggatcaaagt
     6001 cattaaaaca tttggacaag aaaaagaaga tctagcggat tttgaagaaa agattgaaga
     6061 tgcaattatt aaaaacaaac gagtcaattt tttagactcc ttatttgatc cattcattac
     6121 cttgattatc ggtctgtctt atgttgcaac aattatttta ggtggaaagt atgtcttgga
     6181 aggcacgatc tcgatcgggc agatggtctc atttattagt tatatcggta tgcttgtttg
     6241 gccgatgttt gcgattggtc gattgttcaa cgtattggaa agaggaaatg cttcttatga
     6301 tcgcgtcaat gaacttttgc atgaaaagac gcatatcgtg gaacaactgg gtgcagtgca
     6361 gaccccggca aaaggcgagc tgaatgtagc tatttcctct tttaattatc cgaaagaaga
     6421 atcaccagca ttagaacaaa tcaagtttgc gatccaagaa ggagaaacat taggtattgt
     6481 tgggaaaact ggtgcgggaa aaacgacgat catcaaatta ctgatgcgtg aatacgatga
     6541 ttatcaaggg aaaatccaat ttggcggacg ttcgatcaaa gaatattcat tgaatgcttt
     6601 attaggatca atcggctatg tgcctcagga tcacttcttg ttttcgatga ccattcgaga
     6661 taatatccgt ttttctaatc caaaactttc agaagaagaa gtagagcatg cggcttcttt
     6721 agcatttatc aacaatgaaa tcaaggaatt accaaaaggt tatgatacct tagttggaga
     6781 acgtggagtc tctttatctg gcggacagaa acaaaggatt tcgattgctc gagcattgat
     6841 caaaaatcct gaattattaa tattagatga tgcactctct gcagtagatg ccaaaacaga
     6901 agaagctatc ttgcataatt taaaagaaat aagaaaagag aaaacaacta tcattgcagc
     6961 tcaccgttta agtagcgtga tgcatgcaaa agaaatcatt gttgtcgatc aagggaaaat
     7021 cattgaacga ggaacccatc atgagctgct tgcactaggc ggctggtatg aacaaatgtg
     7081 ggagaaacag caattagaag cgaagataga aggaggcgag gcctgatgga tgaaaattat
     7141 caatcggaat ggacaaaatc gattccactt aaagaacaga aaaagatcat caaacgactg
     7201 atgaagttcg ccaaaccttt tagaagaacg ttcatcgttg ctatcttatt tgcgtttgct
     7261 ttgtctgtga tcaatgtcct attgccaagg atcatccaaa cttttatgga tgatcatctg
     7321 gctaaacagt cggctacgac acaagttatt ttgttttttg ctggtatgta cttatttgga
     7381 gtcatagtaa aaagtatcat ttggtttttc caatggtatt tgtattccat ggcctcattg
     7441 aagacctatc agtatattag ggtaaaatta tttgaaaagc tgcatacact aggtatgcgt
     7501 tattttgatc agacaccggc aggatcaacc gtttctcgag tgacgaatga cacagaaacg
     7561 ctatttgaat tttggtacgt ctttttgatg gtattgactg ggatctttgc agtcgtttct
     7621 tcattccttg ccatgtttca aatcaatgga gaaattgcct tgtggacttt ggtgtttttg
     7681 ccgattttag gaatcgtcat ctggtattac cagaaattca gttctcgtat ttaccgaaat
     7741 atgcgggaaa aattaagtca gctgaacacg aaattgaatg aatcgatttc cggcatgcgt
     7801 attatccagc agtttcgcca agaagctcgt ttagctaaag aatttgaaca agtgaacaat
     7861 gagtatttag acacgcgtta tgcgatgatc aaaacgaatt cgattctgtt aagtccgatc
     7921 atcaattttc tatatgcatt ggcgattgcg ttggtgttga ctttgtttgg gatcgatgcg
     7981 ttgcattctc ccgtagaagt tggactgatc tatgcattta cgacctatgt ccagggattt
     8041 tttaatccaa tgtctcaaat gatggacttt ttgagcatct tcacagatgg gatggttgct
     8101 ggtagtcgta tcttgaagat tatggatacg gaagaatatg caccacaaca aaaccctgga
     8161 gcagatcaaa tgattacttc aggtaagatt gagtttcgca atgtaagctt ttcttatgat
     8221 ggagagcaca atgtattaaa caatatctct tttgttgcta atccgggaga gaccgttgca
     8281 ttagtgggac acaccggaag cggaaaaagc tcgatcatca acgtattgat gcggttttac
     8341 gaattttatg aaggtcagat attgattgat gatcatgaca ttcgagaata tccaatggaa
     8401 gagttacgta agaaaatggg gctggtcttg caagatgctt tcttgtttta tggggatatc
     8461 gcaggaaata ttcggatgat gaatccagct atcacagacg aacagatacg ccatgcggct
     8521 gagtttgttc aagcaaacaa attcatcgat gaattaccaa aaggatatca ctcaaaagtg
     8581 atcgaacatg gtgccagcta ttctagtgga caacgacagc tgatcacttt tgcgcgaaca
     8641 atcgtgactg atcctaaaat tctagtattg gatgaagcaa cagcaaatat cgatactgaa
     8701 acagaaggat tgatccaaga aggattggca aaaatgcgtc aaggacggac aacgattgcg
     8761 attgcccatc ggctgtcaac gattcgagat gcagaattga ttttagtttt agacaaaggt
     8821 catatagtag aacgcgggaa tcatgaaaca cttctagcgc aaaatggttt gtatgcagat
     8881 atgtacaaac tgcaaagcag tggtatataa aagatgttgt gacagtagaa ttttgaccta
     8941 agcactagag tgaaaataaa acatagcttc tcatattttt catctgtgag agttagtgtc
     9001 gtagtcacca gatttaaaaa aagattcgac agctagtcag aagtgcctaa tagccactaa
     9061 gaaagcgttg acaaaagcat ttgctttcga taatataaag aaaacgatac aacttggata
     9121 gtgattgcct gatgcaagct aaacctgatg agtctcccat ggtttcatgg acggcttttt
     9181 gggtttttct ttttgttttg gatacagccc tatacatcat ggaaaggagc aaaacatgct
     9241 gattgagaaa gtactgaata acaacgtcgt aataaccagg gatgaaagaa atcaggaaat
     9301 gatcgtgatg ggaaaaggtt tggcattcaa aaagaaagtg ggtgacccga tttttgactc
     9361 atgcatcgac aaaaaatttc agttgacaaa tgacgggctt tcacaacagt tccaagaatt
     9421 ggcacaagcg attcctcttg agtacttaga aatttcttgt gaaatcattg aatatacaaa
     9481 agaggctcta caagtccagt tgaatgattc tgtgtatatt gcattgacgg atcatttgta
     9541 tacggctatc gaacgtcaca aaatgggaat atttgtacca aatgtattgc tgtatgatat
     9601 caaacaacta tttcctaaag agtatgccat cgggaaaaaa acagtgaaga aaatctcgga
     9661 taaataccac atcaagttat cagaagatga agccggattt atcgcattgc atttagtcaa
     9721 tgcacaaggc attcaaagcc agcaagacaa tacctacctg tttactataa caatacagga
     9781 tattctaaat atcgtccgct attactataa gattacattt gatgaagatt cagtccattt
     9841 ttttcgattc gtcacacact tacgtttttt tctgacacgc gtcctagatg gagaaaaatc
     9901 agaagtggga gaagtttctg aggaattgtt tcaggttatt caaaaaaaat ataaggatac
     9961 gtttggttgc gttgagaaaa tagcagactt tttagccgag acgttcaact accagatgtc
    10021 cacggacgaa aaaatgtatt taacgatcca tactgcgcgt ttagtgcatg agaatcgttg
    10081 taaataaaaa aaggaggata aattaacaag aacgtatgaa ataagggtag tgacattcag
    10141 ttggctaaac cttccactaa taaattggag ggaatagaaa tgggaaaata ccaagaatta
    10201 gcgagaaaaa tcgtagaaaa tgtcggcggg aaggaaaata tcaatggttt gacacattgt
    10261 atcacaaggc ttcgtttcaa attaaaaaac gaagaaaaag caaatgatga gatcttgaaa
    10321 aatatggacg gtattgtaac ggtaatgcga gcgggagggc agtatcaagt tgtcattggg
    10381 aatcatgtgc cagtcgtgtt tgaagaggtt ataaaagcag gaaaccttac ttttgatgag
    10441 gctgtttcaa cagaaaaaat gagaccgttt gatatgctta ttgatggaat atcgggctgt
    10501 ttccagccat tccttgctat cctagctgct ggcgggatga ttcgtggact gacagcattc
    10561 cttgtcttct taggcgcgtt tgaccgcggt tctggcacat ttgttatgtt tgataatatc
    10621 ggtgattctg tattccaatt tatgcctgtt attatcggtt taacagctgc ccgtaaattt
    10681 aaagtaaatg aattcgtagg gatgttgctt ggcgcagcat taatgaatcc tagcttgtcg
    10741 ctggaagctt tgagtggtgc agcagaagca ccactaacga ccatattttc tggcactatt
    10801 tttgaagcac ctatttatca aacagtattc ggcattccct ggattgcgcg taactacgct
    10861 tcttctgtca ttcccattat ctttattgtg ttgttagcta gccaaattca gaaaccaatt
    10921 aaaaagttag tacctgagat gatcgcgaat ttcttcgttc cattcttcac cgttcttatt
    10981 acaatgcctt taggtttttt actcgttgga ccagtattca cattcgcaac tgatatttta
    11041 atggctggat ttgaaacact tttagcactt tcgccagtta tttatggagc aatcgtaggc
    11101 ttcttctggc aaattctggt gatgtttggt ctccattggg caatcgtacc aatgggattg
    11161 atgcaatttt ctgtgaatgg ttggcaaaat attatgacac ctgttgcagt agtgtctttt
    11221 ggacaaactg cagcacttac cgcactctac tttaagctta gaaatccaaa agacaaagca
    11281 attgcaatac cagccattgt ttctggtatt gtggggatta cagagccagc tatttatggg
    11341 tttacattac cacgtaaaaa aatatttatt tttacctgtg tcggtggtgc gttagggggc
    11401 gcgtatagtg ggttaatgaa tttaacttct tggaatcaag gtggactagg catctttaca
    11461 attcccaact atattagacc agatggtgat ttaacagatg tgattaatgt attgattggg
    11521 atcgccattg cgatggtggt atcctttacg ctgacttttt tcttttggaa ggatgaagca
    11581 ggtgaaacag atgatataca gaaaaaatca ggtaaagaaa ttgtaaaaac accgatacaa
    11641 ggacaaatcg ctccactaaa tgcagcaaag gatgcagctt ttgcacaagg aacactaggt
    11701 cgaggaatct tgatctaccc tgaaaaagga gaagtacgtg ccccttttga cggaacagtc
    11761 atgaccctgt tcccgacgaa acaggcaatt ggaatggttt ctgaaacagg gctggaattg
    11821 ctgattcatg tcggattgga tactgtgcag ttggaaggga aatattttga aagcttagtg
    11881 cagcaaggat aaagagtgaa acaaggcgat ctgttgctac gcttcgacaa aaagaaaatc
    11941 gaaaaggaag gatacagttt agaaacacct gtgatcgtca ccaattacac ggattatttg
    12001 gatattttgg aaaaccaagg ggagacggtc gggccagacg atgcacttat caccgcactg
    12061 acctgaacgg ttaggtgaag aatggactag gcttaactat tctttttatt ccacttcttt
    12121 attttcaaaa ttgaaaggct ctttcatgat ggctgagctg atagggcgtt tatcgaattt
    12181 ttgaccgaca gaaattagat ggactaaatt ctgttgataa ttttctgcaa aaacacaact
    12241 atataacacg tctagaagat aacaaattga tgtattgatc gtaaaattac caatttttga
    12301 atacagtcgc tcgcgtgtcg taagtcgcaa agcggctgta ctcattgcag atagcgttga
    12361 atcaccgata ctggtcaaag ctaaaattgg aacatgattt tgtttcaata tttctgcggt
    12421 acgaaggatc acattgtttt cacccgtata agaaatcaga atcccgcaca tatcactcgt
    12481 gcaattgtac gcttcataga atgattcacc aaaagtggtg acaatcgtga catccatttt
    12541 gatgcgcttc attttcagca tgaaatcttg agaaatcaaa gtattcgagt tactggcgaa
    12601 aatcacgatt ttttttgctt gaagcattag ttgtttggct ttttgcagtt ggtcatgatg
    12661 aatgagagaa agtgtatcat gaatcgtcat ctgttctaaa gaagccattt tatttgcgat
    12721 cgtcataata ccgtctgtgg tggaaaaagg aagattcgca tctacgtctt gaaaattccc
    12781 ttcaagatag gctgtttcag ctaaaaaagc attacgaaaa ctgacccagc catcaaaacc
    12841 catttttttt gctacacgga tgagtgtaga ggggtggaca taacatcgct cagcaatcgc
    12901 ttggatggtc atttcgccaa gtgctggaga atcgcttaag agaaactgga tcacttcatt
    12961 ttctgaagga gaaaaggaga tttctttgat tttttcacgt aataacatgt agataccttc
    13021 ttcttatttt tttacgtaaa ttataacttt tttgaaaatg atttgtaaac ggatttatac
    13081 tatataactt aaaatagaca ttttttgaca agatatttac aaaaatccac tagaaaacag
    13141 taaatt
//
LOCUS       DACDSY010000078        13024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDSY010000078 DACDSY010000000
VERSION     DACDSY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.079"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(255..3908)
                     /gene="rpoC"
                     /locus_tag="IWK44_001837"
     CDS             complement(255..3908)
                     /gene="rpoC"
                     /locus_tag="IWK44_001837"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP9550341.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3987..7604)
                     /gene="rpoB"
                     /locus_tag="IWK44_001838"
     CDS             complement(3987..7604)
                     /gene="rpoB"
                     /locus_tag="IWK44_001838"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9550342.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8332..9300
                     /locus_tag="IWK44_001839"
     CDS             8332..9300
                     /locus_tag="IWK44_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9550343.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9318..10223
                     /locus_tag="IWK44_001840"
     CDS             9318..10223
                     /locus_tag="IWK44_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9550344.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10220..11026
                     /locus_tag="IWK44_001841"
     CDS             10220..11026
                     /locus_tag="IWK44_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9550345.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            11183..12151
                     /locus_tag="IWK44_001842"
     CDS             11183..12151
                     /locus_tag="IWK44_001842"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP9550346.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12468..12992)
                     /locus_tag="IWK44_001843"
     CDS             complement(12468..12992)
                     /locus_tag="IWK44_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP9550347.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
BASE COUNT         3601 a         2860 c         2577 g         3986 t
ORIGIN      
        1 tcaataatag ctagcaaaat attaaaaata accgctctat cttcccatca atttattttg
       61 cattcaaaaa atgatctatc tttacttttg ttgattgact tctcttggaa gatgatcgtt
      121 ccgatacctt aattggatac gaaccgatgt tgttaagatc ctttcccact tttcttctag
      181 ttagacaaat tattttagac aaaccaaaaa agaggttgtg acaataattt gtcacaacct
      241 cttttttaaa atatctattt tgtttgattg ttcattgcgt cttctgcagc catttgcgct
      301 tcaatatctg aaatactgta gacattttcg cttgctacac cgacttcttt tggttccatg
      361 tcacgataac gttgcatacc agtaccagct gggatgatct tacctataat aacattttct
      421 ttcaaaccaa gaagaggatc tttcttaccg cggatcgcag catccgtcaa gacacgagtt
      481 gtttcttgga atgatgcagc agataagaaa ctatttgttt ctaatgatgc ttttgtgata
      541 cctagtaaga caggtcgaga agttgcagga acgcctcctg agactagcgt attgtagttt
      601 tgatctttaa agtctgcgat gtctaataat gtacctggta agatatctgt atcacctgga
      661 tccattacac gaactttacg cagcatttga cgaaccatta cttcgatatg cttgtcgccg
      721 atttctaccc cttgcatacg gtaaacacgt tgtacttcac gaagcaagta gttttcaaca
      781 gataatacat cgcgaacttg caacaattgt tttggatcga ttgatccttc tgtcaatggt
      841 gcaccacggt ggatcatatc gccttctgct actttcatac gtgctgtata aggaacagta
      901 tacgtacgtg tatctgtttt tcctttgatt gttacttctt tggtacgtgt tgcaggatct
      961 tctgagatat cgatgacatc ccctgttact tctgtgatca ctgcttgccc tttcggatta
     1021 cgtgcttcaa agatttcttg gacacgagga agaccttgag tgatatcgtc tccggcaacc
     1081 ccacctgtat ggaatgtacg catcgtcaac tgagtaccag gttcaccgat tgattgagcg
     1141 gcgatcgtac caactgcttc tccgacttca acgtcagagc cagttgcaag gttgcgtccg
     1201 taacaatgtt tgcagacacc atgttttgta ttacatgtga agacagaacg gattgtcact
     1261 gtttcgacac ctgcatcaac gatttgtttt gcgatgtctt cggaaatcaa ttggtttcct
     1321 gcaatgatca attcatttgt ttcaggatga cgtacttctt tacgggtgta acgtcccact
     1381 aaacgatctt ctaatgattc gatgatttca tttccctcgc ggatggcttg gatttcaaga
     1441 cctcggtcag taccgcaatc ttcttcacgg atgattacat cttgggccac gtcaaccaga
     1501 cgacgagtca agtaacctga atcggcagtc ttcaaggctg tatcggtcat ccctttacga
     1561 gccccgtgag ttgagataaa catttccaag acggatagtc cttcacggaa gttcgaaatg
     1621 attggcaatt ccatgattcg accattagga gcggccatca aaccacgcat tccggcaagc
     1681 tgtgtaaagt tggaaatgtt accacgagct ccagaatcag acatcatgaa gattggattt
     1741 ttcgcttcca aaccttccat cagtttttgt tggatgctgt cttttgcagc attccaaaca
     1801 gcaatgacac gttcataacg ttcgtcatct gtgatcagac cacgacggaa ttgttttgta
     1861 atagtttcaa cttgtttatg ggcattttca atgatttctt gtttttcatt cagtacactg
     1921 atgtcagcga tccctactgt gataccggca tgtgtcgaat gtttgtaccc taagtctttc
     1981 atacggtcaa gcatcttaga agtttctgtt actttgaatc gtttgaagac ttctgcgatg
     2041 atgtttccta agtttttctt cttgaacggt aatactaatt cttgctcttt gatatgtgca
     2101 ggaatatcag taccagcttc aacaaagtat ttgtccggtg tttggacggt caagttgaag
     2161 tctgttggtt cattcagata agggaattca ggaggcatga tctcgttgaa gatgatctta
     2221 ccaactgttg tgatcatcgt acgttctttt tgccattcag taaatggttt ttcacctaat
     2281 gagttagggt tcacaccgat acgtgaatgt aaatgaacat atccgttgcg ccatgcgata
     2341 acggcttcgt tttgatctct gaagaccatt ccttcgcctt cgcgtccttc ttcttccatt
     2401 gtcaagtagt agttacctag aaccatatct tgagaaggag taacaactgg tttaccatct
     2461 ttagggttca agatgttttg agcagctaac atcaacatac gtgcttctgc ttgtgcttct
     2521 tcattcaatg gaacgtggac agccatttgg tctccatcga aatcggcatt ataagcttca
     2581 catacaagtg gatgcaaacg gattgcgcgt ccttgtacta aaactggttc gaatgcttgg
     2641 ataccaagtc ggtgaagcgt aggtgcccgg ttcagcaata ctgggtgttc cttgattacg
     2701 tcttctaaaa catcccatac ttcgtcttct tgacgttcga ttttacgttt cgcatttttg
     2761 atgtttgaag ctagttcgcg ttgaacaagc tcacgcatca cgaatggttt gaataattca
     2821 atggccattt cttttggaag tccacattgg tacatcttca agaaaggtcc aacaacgata
     2881 acggaacgac ctgagtagtc gacacgttta cctagcaagt tttgacggaa acgaccttgt
     2941 ttccctttca acatatgaga aagagatttc aatgggcggt tacctggtcc agtaactgga
     3001 cgtccgcgac gaccgttatc gatcaatgca tctactgctt cttgcagcat acgtttttca
     3061 ttttgaacga tgattccagg tgcattcaag tctaacaaac gtttcaaacg gttattacgg
     3121 ttgattacac gacggtaaag atcattcaag tcacttgttg caaaacgtcc accttctaat
     3181 tgaaccattg ggcgtaaatc tggtggaata acagggataa cgtccataac catccagctt
     3241 ggttgatttc ctgaagcgcg gaatgcttct aaaatatcca aacggcggat tgcacgtgta
     3301 cgtttttgtc cttgtgcaga tttcaattct tctttcaatt cggctgcttc tgcatctaga
     3361 tcaacattat caagtaattg tttaatcgct tctgcaccca tagcagcttg gaattcttgt
     3421 ccatattgtt cacgtttttc gcgatattcg cgttctgtca acaattgttt tttctctaaa
     3481 ttcgtatttc ctggttcgat gactacatat gaagcaaaat aaatgatttc ttccaaagcg
     3541 cgtgggctca tgtctaatac aagacccata cgtgaaggaa tccctttaaa gtaccagata
     3601 tgtgaaacag gagctgctaa ttcaatatgt cccatgcgtt cacggcgaac tttcgaacga
     3661 gtaacttcca ctccgcatcg atcacaaacg attcctttat aacggatacg tttgtatttt
     3721 ccgcaggcac attcccagtc ttttgtagga ccaaaaatcc gctcacagaa caacccttca
     3781 cgttcaggtt ttaaggtacg atagttaatc gtctcaggtt tttttacttc accgtaagac
     3841 cagcttctga ttttttcggg agaagccaaa cctatttgca tactttcgaa tttatttaca
     3901 tcgatcaaaa ggggttccct ccatttcatt taatggcaaa ccgcaagacg gctgatcttc
     3961 gaccgccttg cagctcatag accggattag tctaaattat cttctgcggt ttcaaagttt
     4021 tggatgacat cgtctttttc atcttccact tgttcagcag ctttcttttc taattctttg
     4081 gcagtttgct gttcggcaaa ttttgtcaaa gcatcaacag tgatcaaatc gtcgtcttca
     4141 tcgtccatgt cgcgaagttc gatctctgtt tcttcgatat ccaagacacg catgtccaga
     4201 cctagtgatt gcaattcttt caccaatacg cggaatgatt ccggtacacc tggttttgga
     4261 attggttcac ccttgacgat tgcttcgtat gttttcacac gtccgactac gtcatctgat
     4321 ttgtatgtca agatttcttg taaagtataa gccgcaccgt aagcttccag tgcccatact
     4381 tccatttcac caaaacgttg tccaccaaat tgagctttac ctcccaatgg ttgttgtgta
     4441 accaatgagt aaggtccgat tgaacgtgca tgcaatttgt catcgaccat gtgagcaagt
     4501 ttgatcatgt acatcacacc gacagacaca cgtccatcaa atggttcacc tgtacgtccg
     4561 tcgtacagaa cagttttcgc atcgctggcc ataccggctt cacgaactgt ttcccatacg
     4621 tcttcatcac ttgcaccatc aaatactggc gttgctacgt ggatacctaa ctgacgtgca
     4681 gccataccca agtgtaattc caatacttgc ccgatgttca tacgtgaagg cacccctaaa
     4741 ggattcagca tgatatcgat tggtgttcca tcaggtagga atggcatatc ttcttccggc
     4801 ataatacggg atacaacccc tttatttccg tgacgaccgg ccattttatc cccttcatgg
     4861 attttacgtt tttgaacgat ataaacacga actaacatgt ttacgcctgg tgacaattca
     4921 tctccagctt cacgagtaaa gattttcaca tcgtgaacga taccgccgcc gccatgtggt
     4981 acacgtaatg atgtatcgcg gacttcacga gctttttcgc caaagatcgc atgcaataga
     5041 cgttcttctg cagataattc tgttacccct tttggcgtga ctttccctac aagcaaatcg
     5101 ccatcttgga cttctgcacc aatacggatg atacccattt cgtctaaatc ttttaatgcg
     5161 tcttccccga cattagggat ttcgcgtgtg atttcttcag gtcctaattt tgtatcgcgg
     5221 gcttctgatt catattcttc aatatggata gaagtgtata cgtcatcttt taccaaacga
     5281 cggctcatga tgatcgcatc ttcatagttg tacccttccc aagtcatgaa accaactaaa
     5341 acgttttggc ctagagccat ttcgccttgt tccatagatg gtccgtctgc cagagtatcc
     5401 cctttttcga ctttctcacc caaatgaacg attggacgtt ggttgtagct tgttccggag
     5461 tttgaacgac ggaattttgt tacatcgtat ttatctaatg caccgttgtc gcggcgtacg
     5521 cggatttcag aagcatcaac gtattcaacg acaccgtcat gtttacataa caaagcagct
     5581 cctgagtcat gcgctgattt gtattccata ccagtaccaa cccatggtgc ttgaggattg
     5641 atcaacggca ctgcttgacg ctgcatgttg gcacccatca aggcacggtt ggagtcatcg
     5701 ttttccaaga aaggaataca tgctgtcgca acggcaacta cttgtttagg tgatacgtcc
     5761 atgtagtcta ctttatcaat agatacttct aagttttcac tttgagcacg agccataaca
     5821 acgtcttgag caaatgttcc atcttcattc aatgggctgt ttgcttgggc tacgatgtaa
     5881 tggtcttcga tatcagctgt caagtagtcg atttggtcag taacacgacc tgtttcacgg
     5941 tctacacgac gataaggtgt ttcgataaat ccgaatttat ttactttcgc ataactggac
     6001 aaactattga tcaacccgat gtttgggcct tcaggagttt cgatcggaca catacggcca
     6061 tagtgggagt agtgaacgtc acgaacttca taaccggcgc ggtcacgagt caatccgcca
     6121 ggtcctaagg ctgaaagacg acgtttgtgt gtcaattcgc caagagggtt tgtttgatcc
     6181 atgaactgtg acaactgaga agaaccaaag aattctttga tacttgccac aactggacgg
     6241 atattgatca attgttgagg tgtcaatgtt tcagtatctt ggatcgacat tctttcacga
     6301 accacacgtt ccatacgtgc tagcccgata cggaattggt tttgtaaaag ttcaccgact
     6361 gaacggatac gacgatttcc taagtggtcg atatcatcga cattgccgat accttccatc
     6421 aagttcaaga aatagctcat tgaagcaatg atgtcggctg gacgaaccgt ttttacagct
     6481 gcttctggat agccgtttcc gatcacgttt acttcacgtt cagggtctct tggtgaaaat
     6541 actttgatga cttggacagt cattggatct gtgaccacac cgtcttctga tggatagtaa
     6601 gtcactgaat ttaggccatt atcaatgaaa ggagctagtg tttccatgac ttggtgagtc
     6661 agaactgttc ctttttcaac gatgatctcg cctgtttctg gatcaactaa tgtttcagct
     6721 aatgtcagat tcaatagacg tgttttcaaa tctaattttt tgtttacttt gtaacgacca
     6781 acgtttgcta agtcgtaacg ttttggatcg aagaaacgag cattcagcaa gttacgtgag
     6841 ctgtctgctg tttttggttc gcctggacgc aaacgttcgt aaacgtcttt caaaccttct
     6901 tctgttcttg agtcgcttgc gtttttatgc aagtcttttt cgactgtatt gcgtaatgtt
     6961 tcgctgtcgc cgaagatttc aaagatcgta tcatcggaac caaatcctaa cgcacgaact
     7021 aatacggtta aaggaatctt acgtgtacgg tcgatacgta cataagagat gtcttttgca
     7081 tcagtttcca tttctagcca agctccacgg ttagggataa cagtagaacc gaatccttct
     7141 tttccgtttt tgtcgacttt tccatggaaa tagacacctg gtgaacggac taactgagaa
     7201 acgattactc gctctgcccc gttgatgatg aatgttcctt gttctgtcat taatgggaaa
     7261 tcaccgaaga atacttcttg tgctttgatt tcgccagtct cgcggtttgt caaacgcaat
     7321 gttacatgta atggtgcaga gtagtttgca tcatgcgcac gagcttctgc tactgtatac
     7381 tttggttctt tcaattcata atctacaaat tccaaagata gattgccatt gaagtcatcg
     7441 attggcaaaa tgtcttcaaa catttctcta agtccctcat ctaagaacca ttggtaagag
     7501 tctgtttgaa tttcgatcaa atttggtaat tccaacactt cactgattct tgcgaaactt
     7561 ctacgttcgc gatgttttcc gtattttact acgtgtccag ccaagctctt cacccctcta
     7621 aaatgtcatt caaaaagact acccgatcaa ttgttacaat aatatttcaa atattatttt
     7681 taggtaacaa cagacatttt ttgcggcttt taggcaaaaa aaaaccaaaa atagaacatt
     7741 ctcttgaaac cttacttcgt gagcttctac ttttgtttcc ttatattaaa gccgaaacgg
     7801 acaatatttc gtttctgcct tttcttcatc agatagtttt tgcaacttca tatactacac
     7861 ttattttcta taaaagtcaa cacttcctgg cgtttcttct gctttttcgc aacattttct
     7921 taatgttttc aattttatgt tttcaattat ctgacaattc ttaattgaca gaccttttta
     7981 tctcggttat actttagaaa attcattttg tggaggaact tctatgcgaa ccaacatcaa
     8041 ccaaatcgca ccaacacaac tgaataactt ttactttagc tactttagat aggtttgtat
     8101 tcatcatgat ggatacaaac gttaaacgac agtttgtatc tatgatggct aaagaacttt
     8161 gtattgcagc aaaaagccac gtagacaaaa tgctaagtgg ctgagcagtg aaaatacaaa
     8221 gtaaaagtat caagcattct actttttcaa tttattttcc gacttttcaa tccatttgga
     8281 cattttgtct gaatggattt tttgtttccc aaaattaagg aggaaaaaga aatgaaaaaa
     8341 aatataatca tcggattagg cgctgccctg ctactagctt taggaggttg cgcacaaaaa
     8401 gacaattccg cagaggcatc agatggacaa aaaacaattg gcgtattgca acttgtacaa
     8461 cataactcgt tagatgctgc ctataaagga ttcaaagaag gattagcaga agaagggtat
     8521 aaagaaggcg ataatctaac aatcgattat caaaatgccc aaaacaatca agacaacctg
     8581 aaaagtatga gtgagaaact cgtcaaagaa gatcctgatt tgctacttgg aatcgccact
     8641 ccagcagcac aatctttatt aaatgaaaca accactatcc cgatgactgt cacagcggtc
     8701 acagacttgg aacaagccca tttagtcaca tccaatgaaa aacctggcgg caatgtgact
     8761 ggcacgacag atatggtgcc aatcgaaaaa caaatcggct tactgttaaa tatcgtgcca
     8821 gacgccaaaa caatcggcat catgtataac aacggtgaag caaactctaa gatccaagct
     8881 gatttggcag aaaaagaatt gaaaaaagcg ggcgtcaaag tcaaagtact gacagcaaat
     8941 tccacaaatg atgtccaaca agtgacgaaa agtctagcta aagatgtaga tggaatctat
     9001 attcctactg ataacacgtt cgcaagtgcg gcaagtgttg tcggtgaagt cgcaaaagaa
     9061 acaaaaacac ctgtcgttgc tggcgcagtt gatcaagtca aagacggcgg tctagcaaca
     9121 gtaggaatcg actacgaaag cttgggcaaa caaacaggga tcatggcagc aaaaatctta
     9181 gacggcaaga atccaagtga acttcctgtc gaaacagcga atgatttgga actgtatgtc
     9241 aataaagaca tggccaaagc attaggcatc aatccagaca gcatcaaaga agctgaataa
     9301 aacacaggag gattctcatg acaaatttgc tctctctttt cttatcatct gcatcgcaag
     9361 gggtcttatg ggccctactt gcgattggtg tatacgtcac tttccgtatc ttggacattg
     9421 ccgacttgac tgctgaagga agctttcctt taggtgcagg gatcgcggca gtaagtatta
     9481 caaatggcta tcatccattg acagcttgtc ttctaggctt tttagggggt gcggcagcag
     9541 ggcttgtttc cggactgctt cacacaaaat tgaaaattcc cgcccttctt gcagggatca
     9601 tcaccatgac tggtttatat tctgtcacat cccgtgtgat gggtgcgccg aacatctctt
     9661 tattgggtca gccaacgatc tttcattggg tcgagtcatt cggcataaca aaagacaacg
     9721 ctgtactgat cgtcggtctg ctttttgctt tgatcgttgt atgtttactt gttttgttct
     9781 tacgtacaga aacaggactt gctattcgtg caactggcga taatctggca atgagcgaag
     9841 caaacgggat caacacagat aatatgaaga tcatcggtta tatgatttct aatggatgca
     9901 ttgctctttc tggctcctta cttgcgcaaa acaacggctt tgctgattta aattctggta
     9961 ttggaacgat cgtaattgga cttgcttcta ttatcatcgc agaagttttg ttacgtaacc
    10021 agccactatt tctccgtctc ttgactgtcg ttttaggtgc ggtcatttat cgttttattt
    10081 tagcgttagt cttcgaactg aatgttgaac caagtgattc gaaacttgct tctgctttcg
    10141 tgctagtgat ctgtctttct ttcccgcaga tccagcaaaa attccattta cctcaaaaaa
    10201 agaaaggcgg tagtgtcaaa tgaatgacgt cttaacattg gaaaatctcc atcatacctt
    10261 tgaaaaagga acgatcaacg aaaatcatgt cctgaaaggc attaatcttt ccttagatcc
    10321 cggcgacttc gtgacgatca tcggtggaaa tggggctggt aaatctactt tgctgaacag
    10381 catcgccggt acattttctg tcgatcaggg tcaaatttta ttgaatggaa aaaatatcac
    10441 gaaaaaatct gttgtcgaac gttcgaaatc catcagtcgc gtattccaag atcctaaact
    10501 agggacagcc gttcgtttaa cggtagaaga aaatcttgct cttgctatga aacgaggaaa
    10561 aaaacgcggc ttcttccgtg gtgtcaaacc acaagaccga acatttttca aagaccagct
    10621 ggttcggcta aatttggggt tggaaaatcg tttatcctca gaaatcggtt tgctgtctgg
    10681 aggacaacgt caagctatta cgttattgat ggccacatta cagcgtccag aactgatctt
    10741 attagatgaa cacactgcag cgcttgaccc tgctacttcc aaaacggtga tggaattgac
    10801 agaaaagctg atcactgagc agaatctcac tgcttttatg gtcactcatg acatggaaga
    10861 cgcgatccgc tatgggagtc gactgatcat gctgcatcaa ggaaggatcg tcgtagatat
    10921 ccagcaagag gaaaaacagc agctcacagt gccccaatta ttagcgttgt tcaaacaaaa
    10981 cagcggcacg gcattaaaag acgatcaagt acttttatct tcataatcag aaagaggatg
    11041 ggacaaaaaa tgaacgccca ccctcttctt ttttctcttt ccttctttcg tttttgcttt
    11101 atgtatcaac cgttgtttct tcgaaaacta ttttgttctc tccttcttcc tgatagaatg
    11161 gatgggaaag gagcttgatg ccatgacaac gaaagacaag gtacttcaat tattgaaaaa
    11221 gacaccagat tttctttccg gagaaaagtt ggcacagcaa ctggaagttt cccggacaag
    11281 tatttggaaa gccatcaaag aattggaaaa ggcaggctat cgttttgagc acgggcctac
    11341 agggtatcgt tacctgccct ctgacgtact agatgcaaaa gaaatccagc aagagctacg
    11401 gtcgtttatt cctgatttga aagtacaaac gatgttgact tctgaatcta cgatgaaaga
    11461 tgccaaactg gcagcagctc aaggagattc tgtttcgaag ttatttgttg ctgatacaca
    11521 agaacaagct cgtggacgtt ttggacggcc ttttttcgct gaacctggac gaggaatcta
    11581 catgagtctc ttgctccatc cagacaaacg gtttgatgaa ttgcctcaat atacgatcat
    11641 cgcagccagt gcctgtgtga aagccattga atctgtaacc ggtcgttctg cttcgatcaa
    11701 atgggtcaat gatatctaca tggatggaaa aaaagtctgt gggattttat ctgaagcagt
    11761 cagtgatatg gaaagcggca ggatcagtca tatcatcatt gggatcgggc ttaatttttc
    11821 tgttcctcaa aatcattttc cagctgatct ccaagaaaaa gccatctctg tctttcccga
    11881 cggacaagct agtgtcacaa ggaatcagct aatcagcaaa atctggcaag aattgttcac
    11941 ttatctttct cacccagaaa aagcgttgga cttctataaa gaaaaatcat tcgtattggg
    12001 aaaaaccgtg tcgttcaatc aaaaaggaac gacttatgtc ggtcttgcaa aagaaatcgg
    12061 tgatcatggc gaactgatcg tccaaacaaa tgaacagaag atgagtttgt cttctggcga
    12121 aatcagtctc acatctatcc gtacgcaata attatcagat ttcacaaaag gacacctgat
    12181 ctcatacagt gtcctttttt ctgaattctc ttcaaaagac tctccttatt ctatcagctg
    12241 acagtttctc tatcctctag aaaaaggtat tctaaaaata tctataaagc atggtacact
    12301 ttctttaaat gaatcaatgc aaaaaacaag agagtcatca gtttacacga tcaaaagaga
    12361 tagcaggccg cttccttttt caatcttata gatagaccat gggtaacgat aaaaacaaaa
    12421 aagaacttgc gacttatttt gccgtaagtt cttttttagc tagtgcctta atctcttaat
    12481 cccaacaaac gtctcgcata gatcacctga ccagagtgca tagcagcatc atcaataata
    12541 gaaacgagac gcattcctcg tgtgactggt ggtgtccagt tctcgtcaat tacttcatct
    12601 agctcggaag gttccagcct tcttaagtaa gaaatagcaa aatcagcagc tcctcgcaag
    12661 tatcctagca gatcttctat ttgattagct tggatctgct ttgcttgttc cagactatgc
    12721 ttccaatctt gttcgttatc tgctatcgtc atcggaaacc ggctttccca gtcttgacta
    12781 tgccaaattg gagaagttcc tgctaaatca gcaatctgga aatctaattc tcgagcggta
    12841 tgccaagcca gccaagtcat tgatttgatc tgcggggctc tatcttccac tggaaaacga
    12901 ttggcctcct caatagagac gccttcaaac aaacgttcaa accgatcgta tgcccgatcc
    12961 acactctcgc atagcatatc taaataatcc atttattttc ctccttttta tttatcctac
    13021 ttac
//
LOCUS       DACDSY010000079        12929 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDSY010000079 DACDSY010000000
VERSION     DACDSY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.080"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(189..1025)
                     /locus_tag="IWK44_001844"
     CDS             complement(189..1025)
                     /locus_tag="IWK44_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP9550348.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1036..1965)
                     /locus_tag="IWK44_001845"
     CDS             complement(1036..1965)
                     /locus_tag="IWK44_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP9550349.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2157..2510
                     /locus_tag="IWK44_001846"
     CDS             2157..2510
                     /locus_tag="IWK44_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9550350.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(2584..3240)
                     /locus_tag="IWK44_001847"
     CDS             complement(2584..3240)
                     /locus_tag="IWK44_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9550351.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            3466..5400
                     /locus_tag="IWK44_001848"
     CDS             3466..5400
                     /locus_tag="IWK44_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9550352.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(5486..6709)
                     /locus_tag="IWK44_001849"
     CDS             complement(5486..6709)
                     /locus_tag="IWK44_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP9550353.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(6703..8601)
                     /locus_tag="IWK44_001850"
     CDS             complement(6703..8601)
                     /locus_tag="IWK44_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP9550354.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(8595..9713)
                     /locus_tag="IWK44_001851"
     CDS             complement(8595..9713)
                     /locus_tag="IWK44_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9550355.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(9796..10629)
                     /locus_tag="IWK44_001852"
     CDS             complement(9796..10629)
                     /locus_tag="IWK44_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9550356.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(10622..11539)
                     /locus_tag="IWK44_001853"
     CDS             complement(10622..11539)
                     /locus_tag="IWK44_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9550357.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(11562..11741)
                     /locus_tag="IWK44_001854"
     CDS             complement(11562..11741)
                     /locus_tag="IWK44_001854"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550358.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(11766..12890)
                     /locus_tag="IWK44_001855"
     CDS             complement(11766..12890)
                     /locus_tag="IWK44_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9550359.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         4031 a         2599 c         2154 g         4145 t
ORIGIN      
        1 acatgttagg aataaattgt aattctcttc acaatctttt ccaacacctt tgaaaccgat
       61 atcaaatact ttcaggatgc tgcttctaaa ataaatatca aacaaatgaa tacatcatgc
      121 aaaaaaagcc agacaaccga cgtccttgcg tgtcagctgt ctgagctatg tttatttatt
      181 ttgttctttt aaaaagaacg atacacatac tgatttttcg gagcagagac tttctcagcg
      241 tttgtaacaa ctacagatgg gatttcacgc ttgaatggtt gataatagtc tgattggatg
      301 gttccttcta ttttatacca ttcatcattt ttaaccgtta ctccttccgg catatgcgtc
      361 aacaaaccaa agactcctga atctgccaca caatggataa tgccgaatcg gaacaagaag
      421 atatctgtat tgctatcgtt cggtgtttgg taaacaaatc cttcatagct gatttttttg
      481 ccaatgaatt cgctagggta attataaatt agctccataa cttctaaata gttttcatct
      541 gtgatcttga tagtttgctt atccccatac ttttctttca actcattcat ttggtctaag
      601 tagtctgatt tgttgaaata aatactggta tccggcttta agtattgtgt ctgcatctcc
      661 ggatccccta ctgattcttt acttactggg aaattaaatc ctttggcttc tacgatcgta
      721 gtgtcgagac taactgttgg aaacaacgtc cccacgatta ctggcaaagc tagcagtaca
      781 taagcgattc cacgttgata aggttttgac aacccatggt catgatcgtg ctcgtgtgcc
      841 ttttcttctg cttttccgtt tttgacccac aaatacagct gaactaaagc caaaagagca
      901 gataaaatca ttgaaagaat agctaagtag cgataatgga cattgatata ttgatcgagt
      961 ttgccagaaa cttgcaagta catcatcaat tcgaaatacc cagataaaat caaaaaccgc
     1021 agcatatttt ccctcctaaa taaataatgt gaatccaata acgctaaggg caatcagtcc
     1081 gatcagcgat aaaataaagc gtcctgtaaa atatcgtttc atcatcagta gatttttgat
     1141 atcgaccatt gggccaaaga ccaaaaaggc agctacaggt gctgtgccga acagacttaa
     1201 caaagaagat cctataaacg catctgcttc tgagcaaaga gacatcgtaa aggccaataa
     1261 cagcatgatg ataatggcta aaatctttgt tgagccgagc gttagcataa ttctagttgg
     1321 taaataagtc tgcatcgcag ctgcgattaa tccgccaatg atcaaataac gacctgtatc
     1381 aaaaaattcg tcgattgaat gtgtcaacac atgcccagtt tgatggatca gctgattttt
     1441 tggttcattc ttctcacttt gttcttcttc atggacatga tgatggtgat tttctgctac
     1501 tttagacgtg attttttctt tcaatacagc gtctttttga aaataggcaa gccagacacc
     1561 aatcacaagg gcaacaaaaa agctgcctat cacccgccag cttgccatct gccaagaatt
     1621 gccaaatgcg ataaaagtgg aaaaaatcac gattggattg atgatcggag cagtcagcat
     1681 aaaagcaaac gctgtgtgaa ctggtacgcc ttttttaaca aattgatgaa cgatcggtac
     1741 gatgccgcat tcacatgaag gaaagaaaaa tcctaagatc gaaccagtaa aaatagagaa
     1801 gatacgattt tcaggaagcc atctcttcac acgttctggg gtcaaaaata cttgtaaggc
     1861 gcctgagata agacagccta ataatacgaa aggtaatgct tcaataacga tcgataagaa
     1921 aatcgtactc atctgcaaaa ccgaatttgg aataaaagaa aacatgagtt tcctcctttt
     1981 tatttctagt tcattcgctt tgcaaatata cgcttttatt caaaaaaaac aagttgctct
     2041 gtaaattttt acacaactct tattttttac caaagcactt caattgactt tttctgttat
     2101 tcccattaaa ttaaggtctc atacatatca cttctatcat aaaagggaga ataaaaatga
     2161 aacaaaccac taacttgact gaagttcaag acatactgca acagtcatcc gttgccgtca
     2221 tttatctttc gatgcctaat tgcagtgttt gtcacgctgt tcgtccacga ctagaggaac
     2281 ttctcacaca ttatgatatt ccggcacttc atttggatgc atttgaaaca ccagaagtag
     2341 ctagtaaatt tgaagtgctg actgcgccag ttgtcctgat ttttcatcaa ggaaaagaag
     2401 tctttcggca agcacggttc atcgacttta aaaaaatccg ttatctacta gatgaactga
     2461 cagcagaaga tgattccctt tcttatgaag aaatcttccg aacaaaataa tcgttgtcca
     2521 aacagaaaaa agcgattgaa caagtgaaag ttttgccttc atttgttcaa tcgcttttta
     2581 tttttattct gctgtatttt cggaatgcgc atttgcgtca gaatgcaaga aatagatcaa
     2641 ggtttgcaat tcattcgtca aatcaacatt ttggatcaat acatctgacg gagcattcaa
     2701 tcgagcagcg gtaaagttca agattccttt gattcctgct tccgttaact gattgactac
     2761 ttcttgtgct ctttcagcgg gtatcgttaa aattgctact tcgatttgtt ggatcttgat
     2821 ttgttctgac atatcttcca ttggataaac ggggataccg tctacgatgc gcccaacgat
     2881 ttcctcattc acatcaaaag cacaactgat acgtatgcta ttactttgat ggaatttgta
     2941 ttttagtaaa gcacttccca agttccctac accgatcagc gcaacatttg tcaattgatc
     3001 gtcgtttaat gtctttgcaa aaaagtgcat aagatcttct acatcataac catacccacg
     3061 ttttcctaat tcgccaaaat aagaaaaatc tcgacggatc gttgcactat ctacttgaac
     3121 ggcttcactt aattcagttg aagacacttt tttctttcca gcattattta aaatccttaa
     3181 atagcgataa tacaaaggca gtcttttagc tgtcgccttt ggaataatat gatctttcac
     3241 gaaatgacct ccacgtattg tctaattaca tctttgattt ctccatcata gcagtattta
     3301 taggaaaaaa tcaattccat tgctcagata ttgtgaaatt ttattcttta ttcatttatt
     3361 tatcatttct gcaaatctcg cagtataatg gaatttatgg tagactacta attgaaaaat
     3421 agttttttat ttgaggagaa ttcacaaagt agaaaagagg attccatgat attattacaa
     3481 gccaatcaga ttgctcgcca ttttggtgca gaagtcttat ttgaaaacat acatttagaa
     3541 atcagtacag gcgctcgtgt cgctttagtt ggcagaaatg gcgctggaaa atcgacttta
     3601 ttaaagatca ttgctggaat tgaagcacct gatgaaggaa agatcgctaa aaataaaaca
     3661 gcgactctcg gatacctcgc tcaggacaca ggacttgatt ccaatgaaac agtttggaac
     3721 gagatgctga aagcttttga acaagttaga caaatggaag agcggatgcg cgagctagag
     3781 attttcatta gcgaaggtac gccagacaca cctgcttata gttcttattt aaaagaatac
     3841 gaccggctgc aacacgattt ttcagatcga aacggctatg gttatgaaaa tgaaattcgt
     3901 tccgtattgc atgggtttaa attcgattct tcattttatg atcagaaaat cgatacttta
     3961 tcaggcggac agcgcacacg tttagcttta gctcgcatgc ttttgcaaca ccctgacatt
     4021 ttgattctag atgaaccaac gaaccattta gatatcgaga cactttcctg gcttgagaat
     4081 tatctgcaaa gctatactgg tgcattattg attgtttccc atgaccgtta ttttttagat
     4141 aaagtagtca atgaagtcta tgaattaagc agaagaaaaa tgcgccatta caaaggtaac
     4201 tattcgaaat atctagattt gaaagcagaa cagttagcaa gtgaatggaa ggcttatgaa
     4261 aagcagcaaa cagaaatcca taaattagaa gacttcgttg ctcggaacct tgttcgcgcc
     4321 tctaccacaa aacgagcgca aagccgcaga aagacattag aaaaaatgga ccggctggat
     4381 cgtcctcaag gggatgaaaa acaagcaaac tttctattca gtatagaaaa gccttctgga
     4441 aatgtagtct tgcaaatcga agatgccgct atcggatatg ataatgtcac cttatctgaa
     4501 ccaatcaatc tagatatccg tcgtcaagaa gcgattgctc tagtaggtcc gaatggtatc
     4561 gggaaatcga cgttgctgaa atcaatcatt caccagatcc cttttatccg tggaaaagaa
     4621 acgctaggga caaatgtcac catcggctat tacgatcaag gtcaagctga tctgcataca
     4681 aacaagacga tcctagcaga attgtgggat gagcatccta ccactccaga aaaagagatc
     4741 cgaagtgctt taggtgcttt cttattttct ggcgaagatg tagaaaaacc aatccctctg
     4801 ctaagtggcg gtgaaaaagc acgggttgct ttagccaagt tagcgatgaa caaagaaaat
     4861 tttttgattt tggatgaacc aacaaaccat ttagatattg acaacaaaga agtattagaa
     4921 aatgcattga tcgattatga cggcacactg ctttttgttt cccatgaccg atatttcatc
     4981 aatcggatcg cgacaaaagt cgtcgaactc tctgaaaatg gcagcaaagt ctatctagga
     5041 gactatgatt attatttaga gaaaaagcag gaagaagaag aaatcgccga attgttggca
     5101 caagatgagc cagaatctgc tcctgctccc acgaacaaat ctgattatta ccaaagcaaa
     5161 gaacagcaaa agcttatccg tagccttcaa cgaaaaatta ctcaggttga agaagaaatg
     5221 gcgcggatcg atgaatggat cgatcagttg gaacaagaaa tggtcgcgcc agaaaacctg
     5281 gaagatcacg taaaactcaa tgagctgaac caagatttag aagctgctag acaagatcaa
     5341 gaaacaaaat tgacggaatg ggaagaacta agcttggaat tagaagaata tgaaaattaa
     5401 cgaaaaaacc aaaacagaaa ccattcatgc tgttttggtt tttctttttt tattttatca
     5461 aacaaaaatt ccatatttac ctttttcacc aataagaaga ccaattacta gtcattctca
     5521 ctaaagcttc taaataatag taatcacccc aaatacaagg tgtatttacc ccattatttt
     5581 tgggcttatt ataaacagac tgtgttaaaa agccatcttc ttcttccgtt gtagcatatc
     5641 tttctttcaa ttgtcgcata attttccaaa cagcagactg gtataagttc ttttctgttt
     5701 tgtctaatag acctgattct attaattcta ataatccaca aaccatgatc accatactag
     5761 atgtatcttt ttcttctctg ctagaatcat caaagatcaa atcccaataa caaatggtat
     5821 cttctggcaa ccgattcata aaataattgg ctaatatttt gcttaaatca aaatattttt
     5881 gttctttcaa ataacgataa cttaatgcaa aaccgtaaat cccccatgct tggcctctag
     5941 cccaacagga attatcacta taaccttgat gcgtacttcc atatcttggt tttccatctt
     6001 tgacatccat ataaaatgta tgataagttg aaccatcttc tcttactaaa taatctgctg
     6061 cattttgaat atgagcctga gcagcttttt tatatttttg atttccagtt tcttcactcg
     6121 cccaatacaa taatggaaga ttcatgcaac aatccatgat cattctacct tgttgtgaag
     6181 gatcactagg atctccccaa gcttgtataa ctcctgcctt ctcccaaaaa cgctgaagta
     6241 atgtttctgc agcatcaatt gaagcttcct tcatttttcg atttcccgtt attttataat
     6301 ctgctacggt tgataaagaa tacaaaaaac caagatcgtg atgattagtg ataatttgac
     6361 agtcaatcct gtggataaaa tctatatctg ctttttcagc tgtttgttga aataaaggat
     6421 cacccgtctt ctcaaataac aaccaattaa ttcctgtcca aaatccttct gtccaaccaa
     6481 tattgtctct taatggataa cgtcctttta taggacactc gcttgggaat tgatactgac
     6541 tgttactgat acgtttgatt aacctctccg tgtgtctgcg acaacgctcg atctcattat
     6601 tccattcttt ttccattaat tctttataac attcatttat ccatttttct ggatgtatta
     6661 tctcttcaaa catcacttta tcaagcattt ccccaccagc ctttagcata attttagtct
     6721 ttttttattg tttttatttt ggatagttag cccggttgtc ccatcaaaat caattttggg
     6781 acgattgttc catacttcta aactataatc ccgatcttga tgagcaacca ctcctgttcc
     6841 gagccaatgg gttccttttc caaatgtacc ctctaatcca ggaataaaag tgttttccca
     6901 tgtcatcaag tttgtattgg gcaaacccat aatagctgtg gattttcgtt taatagtatc
     6961 gtcacttaaa cagataatac ctgaaaagcc agcttcactc accctatagt tttccttgtc
     7021 ttcttctatg attattcctt ctttctcagg agaacattca atagcaaacc ctgtctcata
     7081 tagaacatat tctgtatcta attctacttt gtgtacgcga atctgccagc cttcaaaagg
     7141 aataagccaa gttctgattt ttgcctgtaa agtaggttcc catgtagagg caagtacctc
     7201 tttcttacat acaatgtctc gtacattatt tcgaacgatg aactgtccat aggttttgat
     7261 gctctcattc ttaaaactgg tcgtgatata tgtgtccgct cgctgaatcc ctagcattga
     7321 atccattgcc atttctgctt tcaattgatt cgctcgcggt atcgaaaaac caaattgact
     7381 agaataagca aattttaaat atttttcatt ggtcaatgta tggaaatttg gccctaattg
     7441 tcccgcattc aaaaaaaatg tatgtccatt atcatgttgt atgagcaaat tggcttctga
     7501 aagaagttta gttccttcac gaataggcat aggcatttct ttagccgtcc aatactcatg
     7561 gtcttcaggt agcgccaaca ataaaaaaat cttattcagc cataatggag ataacgtact
     7621 attatagggt tcagtcaatg tcaattgagg gtaggtataa ccaatattca ggataccttt
     7681 ttcatcaaat atttcttttt gcatccacca tcttatattc cgattgacta accccttgat
     7741 ttcacctaat ttaaaaggca aaagatcggc ccacaacata gctgaccaaa agcttgaaac
     7801 tgcaaaacga taaatcatgc ttcgaccaaa aggcacattt gcaccatctt ccgcaaaaaa
     7861 atgaatatag tctctagaaa aatcaatagc tcggttctta tatatctggc taatgggctt
     7921 ttctggttct agtacgctat aaatcaaacc ataataatga aatgcaaaag gattataata
     7981 atcttggcga cctcttgaac tgtctcgata ccagccattt tgaagataac aattttctat
     8041 attatcaaat gcttctttca gtgctgattc tttaactgga taaccaaatt tttggataat
     8101 tgttccgaca attatcttaa aaaattgcca attaccatct gcacattcat attcaaaaat
     8161 ttgcgagaac caatgaataa tctggctttt ttctcctgta tctaattttt tccataataa
     8221 ttgttcatga tgatataacg ctaataacat tgcgggcatt tctaccatac gctggtctct
     8281 ttcatgtatt tctccccaat aatcgggatg ggaaggatct gttccagctt ttattcctga
     8341 tagaattaat ttcaaccatc gttcatctaa ttggtcaatt gcgggccccg ctccccataa
     8401 aacccgagag aatccttcca ttcctgcaat tttatttcca tatcctaccc cttgatgtat
     8461 ataatcaatt tgtgcgccac ttgctcgata aaacggctgg agtggttgat ttatcttttt
     8521 aaataagtca aagtaatcct gtctggtcct taatagtggt actggatgtt tcattgtatt
     8581 ttctcctctt tttactacca aatagctaat tccgactgtt ctaatcgaag taagccttca
     8641 ataaaaaaat aatcagaata gatcagtggc acattataag tcagttctct atggtatgct
     8701 tctgctccgc ctccaatgat cccatcttca tccatattcc aatttgcata gtgcttttct
     8761 gtcttttgga gtatattgat agcagcttca tgatacattt ttgatcgtac agaggaaaca
     8821 tatcctgcta attccagaag accgcatgca gcgcatactc cagcactggt atcaatattc
     8881 gaatctttta cagcaaaatc aatgagagga atataaccag ttttttcgat tttctgaata
     8941 aaatttactg ctatcttttc tgctatagct agatagtacg gattttttgt gtaacgataa
     9001 cttaacgcga atccatataa agcccatgca tttcctctgc tccaacaaga attctcattg
     9061 tatccttgcc cgccaagtaa ccgaatatat ttccctgatt ctgcatcaaa ttctgcgata
     9121 tgcccggttg atccatcttt cctcactaga tacttagctg ttgtatctgc atgctgccga
     9181 gcaattttag agaacctcgg atcaccagtt tccctcgaag cccaatacag taatggtaaa
     9241 ttcatcatac tatcaataat catccaacca ggtttatggt cattccatgc tactaaatag
     9301 ttccctgaaa tattatagcg tccagcaagt accgtagctg catgtaaccc tagttgtcga
     9361 ctatgtttgt tttgagactg ttgataatgt gcaatagccg atggtaagaa taaaaaacct
     9421 acatcatgat gaacgtctga aaaatggtac agggcagatt ctaataattg ttcttgttgt
     9481 attgctgcct ctttgaactt ttctcgtttt gtcttgtgaa agagctgcca taaaatacca
     9541 ctccaaaaac cattggtcca ccagtcgatt cctctgtcct ttattacgtc tgtatatttt
     9601 ccgttgtaag aaatatatgg aattttattc cccattctgt ctaattccgc atctatcttt
     9661 tcagaagcta cattcaaaat ttttcttgac cattccttcg tctgttcatt catggatatc
     9721 ccgccattct tttaattttt atgaatgtgg agttgctcct agaacgaact agatggttaa
     9781 cattcgttat tttttttact tatttgcagc tccaatgtca ttctccttaa atttagactg
     9841 cgcccacaca taaatcaaga ccattgggat cactgaagtt gctaaaatta gcataattgc
     9901 gccataatta attggctcac ctgtactaga aaccgtcata tttttgataa ttaagggaac
     9961 cgtatattta tctttatctg aaagcatgac taatggaaat agatattgat tccaacttcc
    10021 cataaagata agtaaaccac tagtaataat cgtaggtgat aaaatcggta cagcgatttt
    10081 cgtaaaaatc acccactcat tggcaccatc gattctggca gcctccatca gtgctgtagg
    10141 aaattgactg ctatattgtc gcatcataaa cactgaagtt gtactggcaa tcgaagggat
    10201 gatcacacct gcataagtat tcgataatcc taaacgactc ataatgtaaa ataaaggaat
    10261 caatgtggta aaaaaaggca ccatcctaga tagcataact gtattgaaca acagtttatt
    10321 ccccataaaa cgatactttg ctaatgcata acccgccatt gtagaactgg ctgtagctaa
    10381 aactgtactg attacagcaa taacaatcga attccaaata atcaagggat aattatatct
    10441 cgccatgatg gcttgccagt tttttaatag catatcccca aacttgaaat tgattccgcc
    10501 acttaaaatt tcttgattac tttgcgttga agctacaatc atatataaat atggcatcaa
    10561 aaatattaag gcaccaaaca caataatgac gtacgtaaaa attctgcttt gtatcttttg
    10621 tttagtcatt tttatcacct ccgaatttaa attgaatagc cgataatata acagatacaa
    10681 acaccatcgt ccaggcaata gcagaggctt ttccaaaatc aaccatttca aaacttgttt
    10741 tatataaatt gactcctacg accaaagctg aattttctgg tccattggaa gtcccaaaga
    10801 atatatttgg gatattgaaa ttacccaagc cattgatagt ggctaaaact gtggccatta
    10861 aaatgattgg ctttagcatg gggatcgtta ataaaaagaa ttgccgaaat ttcgatgctc
    10921 catctatttc agcactttcg tatatctctt tagaaatccc ttgaagacca gctaaaaata
    10981 aaatagtgtt atatcctaca ctaagccaaa tcatcgtgat gatcattgat attcgtatcc
    11041 aaaaaggatt tgttaaccac tgtacaccgt taattcctag cgcattcaaa aatccattga
    11101 ataacccagt cggattgaaa aaagtcatga atacggaagc tacagccacc gttgatgtaa
    11161 tggttggaat ataatagaca gttctaaaaa atccttttcc ttttaactgt gctgaattca
    11221 ataaaacagc aaagtaaata gctaatagca tcgttattgg aattgatcct gccatgataa
    11281 tcaaaacatt cgtgtaagat ttaataaaca atggatctcg gaagatagct agataattgt
    11341 caaatccaac aaattcaaat gagtagccaa ttttcataaa actaaaagca aacgagataa
    11401 tgatgggaat caaaatcgtc acaataaaga aaataacaaa aggtgtgacc aatccataag
    11461 cacttttttt atgagacgta tctgttgctt ttttcttgct gttctttatt gtcttgcttg
    11521 gtaacgaaga ttgcatcatc gttaagtcct cctaatattt tttaatcacc agttaatttt
    11581 acgccgtact tatgagcaaa attttctgct tcttttagat atgaattttc aaaatcacct
    11641 gtttgccaat agttatatgc agcggtagtc aaataagacg tagcatctga ataagtggct
    11701 ggtaaaacag tatttgatgt attttcattg attttattca atagttcata gtatttttgt
    11761 cctccttagt agtcaaactt tttctcagct gcttctgttt gataggccaa tgagttagat
    11821 gtagccacgc ccagctccag tgcgttggct aagcattctt catctgtcag cataaatgta
    11881 agaaattgtt gtgccacttg tggatttttg gcatctttag ggacataaaa acttgatcct
    11941 cctgatactg caccttttcc tggattttct tttgaaaaag gaatcagtgg agccatgcgc
    12001 catttgtcag atgcatcagg aaaattcaaa gacatattcg tagcatacca gccgccagca
    12061 acaaacattg cagattcttg aaatgctttc tcagcatctt gtgcaccgta gaaatgtaca
    12121 atatctgagt cgatcatttc tttgattaac tttgcagcat gtttcgcttc tttagtattc
    12181 agacggatat ttccttcttc atcaagcaaa gattgattct gctgggctag catgttgata
    12241 aacaattctt ggtccccaga ttctggcaag gcaattccaa aactaccagt tttttctttt
    12301 aatgcttttg ccacctggat atattcttcc caagaactga tttcttcaaa atctacacct
    12361 atttcagcaa atttttttgg ttgataataa caaagtgctg gggtaaaatc actagcccaa
    12421 ggaataatcg tttcattact cgtttgttga cgcaaaatat tcaatttttg atcataaaac
    12481 tgatcgccat atttgtcata aaatccatat tcatcagcac tataaaaagc atcttgaaat
    12541 ttttccagca cacctgctgc tgaagtgtct gccatggaga taatatccgg catatcttcg
    12601 tgtgttacca attttggggt gatcatttgt actgttgctg ttcccgaagc tacattcaca
    12661 atttttaagt caatatttgc tccagttttg cggttataga tttttgcagc attttcaaca
    12721 tagtttgtca gtttgccgtt tgcgacatat acttctaatg tagtggcttt ttttatcatt
    12781 ggatctgcga tgtttgctgt tacttcttcg ttagtttttc cacatgcact taagctgaat
    12841 aatagggtaa tgtttagcaa agctatcacc aatttgtatc tttttttcat gtctatccct
    12901 tctttctttt caagttctta tataaaacg
//
LOCUS       DACDSY010000080        12734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDSY010000080 DACDSY010000000
VERSION     DACDSY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.081"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWK44_001856"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWK44_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9550360.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IWK44_001857"
     CDS             complement(322..891)
                     /locus_tag="IWK44_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP9550361.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IWK44_001858"
     CDS             1077..1979
                     /locus_tag="IWK44_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9550362.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWK44_001859"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWK44_001859"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP9550363.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWK44_001860"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWK44_001860"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP9550364.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWK44_001861"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWK44_001861"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP9550365.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IWK44_001862"
     CDS             8547..8732
                     /locus_tag="IWK44_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP9550366.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IWK44_001863"
     CDS             complement(8794..9990)
                     /locus_tag="IWK44_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9550367.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IWK44_001864"
     CDS             complement(10160..11596)
                     /locus_tag="IWK44_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9550368.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IWK44_001865"
     CDS             11889..12698
                     /locus_tag="IWK44_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9550369.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DACDSY010000081        12576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDSY010000081 DACDSY010000000
VERSION     DACDSY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.082"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(200..562)
                     /locus_tag="IWK44_001866"
     CDS             complement(200..562)
                     /locus_tag="IWK44_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550370.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(636..1016)
                     /locus_tag="IWK44_001867"
     CDS             complement(636..1016)
                     /locus_tag="IWK44_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP9550371.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1098..2273
                     /locus_tag="IWK44_001868"
     CDS             1098..2273
                     /locus_tag="IWK44_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9550372.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2350..2934)
                     /locus_tag="IWK44_001869"
     CDS             complement(2350..2934)
                     /locus_tag="IWK44_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9550373.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3135..4133
                     /locus_tag="IWK44_001870"
     CDS             3135..4133
                     /locus_tag="IWK44_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9550374.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4245..4754
                     /locus_tag="IWK44_001871"
     CDS             4245..4754
                     /locus_tag="IWK44_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP9550375.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4784..5443)
                     /locus_tag="IWK44_001872"
     CDS             complement(4784..5443)
                     /locus_tag="IWK44_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP9550376.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5445..6209)
                     /locus_tag="IWK44_001873"
     CDS             complement(5445..6209)
                     /locus_tag="IWK44_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP9550377.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6217..6894)
                     /locus_tag="IWK44_001874"
     CDS             complement(6217..6894)
                     /locus_tag="IWK44_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP9550378.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6931..8316)
                     /locus_tag="IWK44_001875"
     CDS             complement(6931..8316)
                     /locus_tag="IWK44_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP9550379.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8526..8610)
                     /gene="ffs"
                     /locus_tag="IWK44_001876"
     ncRNA           complement(8526..8610)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWK44_001876"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8633..9148)
                     /gene="tadA"
                     /locus_tag="IWK44_001877"
     CDS             complement(8633..9148)
                     /gene="tadA"
                     /locus_tag="IWK44_001877"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP9550380.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9255..10112
                     /locus_tag="IWK44_001878"
     CDS             9255..10112
                     /locus_tag="IWK44_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP9550381.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10193..10528)
                     /locus_tag="IWK44_001879"
     CDS             complement(10193..10528)
                     /locus_tag="IWK44_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550382.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10595..11965)
                     /locus_tag="IWK44_001880"
     CDS             complement(10595..11965)
                     /locus_tag="IWK44_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP9550383.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12043..12576)
                     /locus_tag="IWK44_001881"
     CDS             complement(12043..12576)
                     /locus_tag="IWK44_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9550384.1"
                     /translation="MGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYPI
                     YTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAKS
                     DLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWDK
                     NEVTDEYLENILKEQSP"
BASE COUNT         3738 a         2552 c         2109 g         4177 t
ORIGIN      
        1 ataaattaag cattactttg tgaaatgatt tttcgatcaa ttattattat tcgttataca
       61 accgttttca acatattttg aaaataaaat gcactaaaaa actatcttta gcagaacatt
      121 tcattgtatt ttttggacca aacaataaaa aaagaagttt gaaaaaaatt caaacttctt
      181 tgttcgctta atctgtattt tagaagaaag gcactccctc tacttcgtaa gttccaagaa
      241 cagagcgcat ttgacctggt tcttcccaaa tatatccttc gcctaattct tctctttgat
      301 tcttttgttc agtttcaaag acaaacaacg gaacattttc ctctttaata tgtgtatttg
      361 tcaattccat caaactaatc ttgctaacat cgatatgaac tatttctttt aagaaattaa
      421 gtatattcgc taaaccagtc tttccttcag aaagttccgc taacgcaaat tccaacttgt
      481 catcaattga atggaccaaa aattttttag taccatcctc caaatgcaac ataattgtac
      541 cagcaactct tttgcttgcc aaaatttcca cctcgatcaa cttgttctat ggcttacata
      601 aaaattctag cacattttca gaaaaaatac actttttatt tcttattttc tttccgttct
      661 agttcagata atgcttcttc tatccaaata ggtaactgct ccgcaatagt ggtaaatccg
      721 atcttacgat ttttattcgt gaaatacctt tttctgcgat atggaacagc tggatttgtt
      781 ttttctaacg ttcttaaaga taaactgatt tttttggaat attcatcaat gtcgatgatc
      841 tgaactgtta ctggctgtcc gacttttaac aatgaatgga tatttttcgt ataaccggat
      901 tgtacttctg aaacatgaat aagtccttgc gtctcgttat caagagaaac gaatgcgcca
      961 taaggctgta tccctgtcac ttttccttca attatttgtc caattttata agtcatctac
     1021 ttccctctct tcgcatttat cattatactg taaatagctc aaaaaaagaa gcgacgaaaa
     1081 tgactggagg tcttaagatg atcgaatttg ataaacagat ttcaagaaaa gatacagaaa
     1141 gtgtaaaatg ggacgcaatt gctgagacat atcaaatgga tgacttactt cctttgtggg
     1201 tagcagatat ggacttcttg tctcccggtg gggtagctaa agcgtttagt gaatacatca
     1261 aacatggaat ctttggttat gccactctgt ctgataaact atatcaggct atcattcatt
     1321 gggaacgcca acaccatgct gtggagttga cgaaagaaaa tatcgttttt acaagtggtg
     1381 tgttaaccag tttagctgca gcagtacaaa cttttacaaa accaggcgat tctattttga
     1441 ttcatgaccc agtttatcct ccattttcct ctattataga aacaaatcaa cgtcacatcg
     1501 ttcgaagctc tttgctagaa aaaaataatc atttcatcat ggacttgtct gacatggaga
     1561 aaaaaatcga agaaaacaat gtaaaagcaa tgattttatg caatcctcac aatcctggag
     1621 ggcgtgtttg gagcaaagaa gaattgaaac agttaagcga attgtgttta aaacatcaag
     1681 tgattctgtt tagtgacgag atccatcaag atttaacttt attcgatcat acatttactt
     1741 ccatgctaac gattgatcct aatttagatg atctactagt tgcttttact tccgcaacca
     1801 agactttcaa tctagctgca atcaaaaatt caatggtatt tatcaaaaat ccagagatga
     1861 aagctgattt tgaaaaacat ttagtgatga accaacagca cgaaatcaac acttttggac
     1921 tgattggaac ccaagcggct tatgagacgg gtggagaatg gttaagccaa ctgattcctt
     1981 atctagaaaa aaatgttcaa acagttaatc agttttttga acagtatctt ccgaaagtga
     2041 gaataatgaa ccctgaagga acttacttaa tgtggttaga ttttaccgcc tatatttcag
     2101 acgaccaaga attggagaac actcttgttc ggaaaggaaa agtcgtatta aatcctggca
     2161 tcacgtttgg tccttccggt catgggcata tgcgcttgaa tattgcttgt ccagaagaaa
     2221 ctttactgga aggacttaat cgaatcaagt atgctttaga gacaatcgat taaatcattt
     2281 attcaaaata aaaggacgag accttactag gtttcgtcct ttttcataat tactccgtac
     2341 cataccaaac tattctgaaa tttcaattgt ttcaatcact acatcatgta caggtcggtc
     2401 ttgtggacca cgttgtacat ttgcgatttc atctactgtg tccatccctt ctagtacatg
     2461 tccaaagact gtatgtcgga aatctagcca aggagttccg ccacctttat acgcttcaat
     2521 gatttctgat gggaatccag caccttctag ttgtccaagc atattagctg gcacatttgt
     2581 gttatttaca ataaagaatt ggcttccgtt tgtgtttgga cctgaatttg ccatagataa
     2641 agctccgcga aggttgaaca gttcacgact gaattcatct tcaaaacttt cgccataaat
     2701 actttcgcca cccattcctg ttccagttgg atcgccgcct tggatcatga aatcaggaat
     2761 aacgcggtga aaaatcacgc catcataata accttttttg gaaagttcaa tgaaattttg
     2821 aaccgtcttc ggtgctagtt cagggaataa ttgaacagta atgtcaccac ggtttgtttt
     2881 gataacagct tttggtcctt tttcactttt taaatctagt tgtgggaatg ccatattgtt
     2941 tcctcctaat atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa
     3001 atactagatt tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc
     3061 gatttcgaat tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa
     3121 agggaggaat ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga
     3181 tgtttgccgc tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac
     3241 ctcaacttgg tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg
     3301 ggtatccagc aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt
     3361 ttaaccatac attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta
     3421 tcgagattac tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa
     3481 atggttcatt ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg
     3541 gattggatta tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg
     3601 gaggtggtga ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt
     3661 acttgattca tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat
     3721 cttcatctgt caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg
     3781 aactgacaga tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg
     3841 attctctgat cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac
     3901 ttgatagtac cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg
     3961 tttatgcagc tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc
     4021 tcggtgaagc acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa
     4081 ctcagcctgg tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta
     4141 tatctctttg catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg
     4201 ttaacatgaa atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg
     4261 agactttaga agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata
     4321 tcgcacaact tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt
     4381 gtactgaaag tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg
     4441 gaatccaatt agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta
     4501 tcgaagatga cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg
     4561 tttacggtac gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt
     4621 taaaagaact aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg
     4681 gagcgatcgc cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc
     4741 gccttactca ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct
     4801 tttttgtttg tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta
     4861 ataatacaaa aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga
     4921 ttggatcagt aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa
     4981 ggataaaaaa gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca
     5041 tgccgatttg aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga
     5101 taaacaaaat actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa
     5161 ttttcacttc ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga
     5221 ggatggtttt gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg
     5281 tctcttgatc taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata
     5341 gtggtcgaaa gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga
     5401 acaatccagc atattccatc cactgccatc tcgaattttt catattagaa gctccattca
     5461 tccagtgatt gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt
     5521 gtaaaacctg ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct
     5581 gtttcatagt tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg
     5641 gctttatcca taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct
     5701 gtctctacaa aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata
     5761 ttcttatctg ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta
     5821 actacttttt cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct
     5881 tcttcccatg taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca
     5941 tagacttttt tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg
     6001 tagactgtct ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt
     6061 tctggtgact tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat
     6121 tcttccacaa atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct
     6181 aaatcaatta aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt
     6241 aaatgctgtt tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga
     6301 ggtttttgct gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac
     6361 tggtcctttt ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg
     6421 agttgtcttt tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt
     6481 gcaatattcg taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg
     6541 attatccgct tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat
     6601 ataatcatat cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc
     6661 ttcacgataa tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc
     6721 taacagtgtc actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc
     6781 caaagcagct gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt
     6841 agcaatttct gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc
     6901 accacctatc ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta
     6961 atctcctacc acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc
     7021 aatcgttgga aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt
     7081 attcagatcg attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat
     7141 tccattatag accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata
     7201 gttacgtaaa aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc
     7261 acccaatgtg acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg
     7321 attgacccgc ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc
     7381 cgtctttcct cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa
     7441 gatatctaga ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg
     7501 cccacgttct agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc
     7561 ggtagggatg gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg
     7621 gacttcaccg acatactgcc cgtactttcc agcggctgct atttggacat cattgattac
     7681 gtgaccttct ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat
     7741 ttcttttgca atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc
     7801 cacttgtgaa cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc
     7861 ccagccattc ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt
     7921 cgtctggtgt ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt
     7981 atttttatcg aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt
     8041 caactcttct ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc
     8101 aacttcattc atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca
     8161 acgatccaca aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt
     8221 ttctcgttta cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg
     8281 cagatcattc gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct
     8341 ttatcatacc ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc
     8401 tgccttgaag caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag
     8461 gaacattcat ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg
     8521 tttcattata atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac
     8581 acgcttcaca agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt
     8641 cattcgttga ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt
     8701 cagtcaaaat catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt
     8761 taaaccgctc gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat
     8821 acgcgccgaa ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac
     8881 aaggctctaa ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac
     8941 aggcttcctt gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa
     9001 ggttatatcc tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt
     9061 catgaagcat ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt
     9121 ctactgcatc aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt
     9181 tactttagca tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac
     9241 aagaggtgaa caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa
     9301 aaaactctcc agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct
     9361 ggatcaaaca agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg
     9421 taatcaacga tcaaaaaaaa cacccatggt atcgctatct ttttaaagat gcttcttgtg
     9481 ttcaagaaaa aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa
     9541 aaaaagaatg gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt
     9601 atacagacac agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg
     9661 aaatcaacgg tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg
     9721 ttggttcttt ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa
     9781 tttttttaac agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg
     9841 tggctttaca ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca
     9901 tagaactgat caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag
     9961 gcaatgtcag ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa
    10021 tcgaaaaatt tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt
    10081 gctatctttt attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa
    10141 ttttgtcaca accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca
    10201 ttttttcaac aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat
    10261 caccataaac agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag
    10321 aatccaagtc actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa
    10381 atttgtcata gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca
    10441 ttacttttcc cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc
    10501 cgattgttac ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca
    10561 tagcatagac aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga
    10621 aaaagttctt tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata
    10681 ctagcaaaac ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt
    10741 gccattcctt ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg
    10801 ctaatgcata gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac
    10861 gtacgacttt taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt
    10921 tgaacactaa aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga
    10981 gtttttcctg ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat
    11041 gcatctgatt ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt
    11101 tatgaggcca tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc
    11161 ctgaactaac ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa
    11221 gccagctaat gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt
    11281 gtcctttatt ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac
    11341 agtatagcgg agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc
    11401 cttctcctga acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga
    11461 caagttctgc gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat
    11521 tttctgataa aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc
    11581 ccttcatttg agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa
    11641 ctttttcacc gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc
    11701 tatattcata cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct
    11761 cattgctgta gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc
    11821 ctgccggttt tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag
    11881 aaaaaaaatc ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg
    11941 gtaacgttgt cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt
    12001 acgaaaaata aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt
    12061 taagatattt tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat
    12121 ttcaagaaaa tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac
    12181 caattcaagc gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg
    12241 ccgcttaaat agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc
    12301 atggatagaa aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca
    12361 ttcagctaag tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc
    12421 gctgattgat ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa
    12481 ctctcttgca gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag
    12541 caaatattct ttactataat ttgttactct ccccat
//
LOCUS       DACDSY010000082        12475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDSY010000082 DACDSY010000000
VERSION     DACDSY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.083"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            59..1942
                     /locus_tag="IWK44_001882"
     CDS             59..1942
                     /locus_tag="IWK44_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9550385.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2017..2301)
                     /locus_tag="IWK44_001883"
     CDS             complement(2017..2301)
                     /locus_tag="IWK44_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9550386.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2291..3025)
                     /locus_tag="IWK44_001884"
     CDS             complement(2291..3025)
                     /locus_tag="IWK44_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP9550387.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3154..3777
                     /locus_tag="IWK44_001885"
     CDS             3154..3777
                     /locus_tag="IWK44_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP9550388.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            3969..5090
                     /locus_tag="IWK44_001886"
     CDS             3969..5090
                     /locus_tag="IWK44_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP9550389.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5097..8225
                     /locus_tag="IWK44_001887"
     CDS             5097..8225
                     /locus_tag="IWK44_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP9550390.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8244..11438
                     /locus_tag="IWK44_001888"
     CDS             8244..11438
                     /locus_tag="IWK44_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9550391.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11584..12291)
                     /locus_tag="IWK44_001889"
     CDS             complement(11584..12291)
                     /locus_tag="IWK44_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9550392.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4249 a         2203 c         2632 g         3391 t
ORIGIN      
        1 ttcacttaat acccgtatat tacgaatgaa aaaagtttat gaaaagttgg aatattgaat
       61 ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact gggtttgatg ggctccgttc
      121 attagctgtg gtgggtgtta tcttctatca ccttctgcca actagtatga aaggcggcta
      181 tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg atcacggatc tgctgcggca
      241 ggaatgggaa caaaatggaa aaatcaatat ttggcaattt tatgtcagaa gaatgaagag
      301 actctatccg gcacttgttt ttatgcttgt gacagcttcg gcttatatca ctttgtttca
      361 gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa
      421 ctggtggcaa atcaataacg gtttatctta tttcgatcga tttgctaatg agtcaccgtt
      481 tacgcatatc tggtctttag cagtagaagg tcaaaactat ttggtttggc cgatcttgtt
      541 tgtattgttg atggtatttg ttcgtaaaag aaaatggatc ttttatactg tgttaggtac
      601 atccttcctc tccgccatct tgatgatgat cttgtataca ccaggaggcg atccaacccg
      661 tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt
      721 tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa caagcgaaaa atattctgaa
      781 tatcgctgga ggaatctcgc ttttggcact gattctttgc ttcttctttt tagatgatca
      841 ttacaatttc atctattacg gaggaatgct cctagtgagt ctgttatgcg tagtgctagt
      901 tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg ctgacaaatc cagtatttac
      961 ttggattggc aaacgaagct atggcattta tctgtatcag tttccagtta tgatttttta
     1021 tgaagcaaag gtgactgata ttgccgatca tgttttgttg catactttag tggaaatcac
     1081 attgattcta atcatcagtg aactttctta tcgatatgtg gagcgtccgc tagcacgttt
     1141 tgattatggt cgtacattcc aagtgatgaa aggatggttc agcaaaccga tcatcagtaa
     1201 gagcaaacca tgggttgttc caggcacatt acttgtagca attgcactcg ttggtttcgt
     1261 tacggcaccg aaaaacgctg taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga
     1321 aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa aacaccacta gttcggttga
     1381 ccaagcgatc cttgataagt acgagttgtc caaggcacaa gggaataagg cacaaaaatt
     1441 agaactaacg gcatttggtg attcagttat gttggatgct gcaacggatc tacaagaact
     1501 ttatccaaaa gtagtggtag atggagatgt cggacgacaa ttatatgcca gtgtaccgta
     1561 cattgaaaaa ttaaaggaac aagatttgtt gaaagacact gttctgattg gactaggaac
     1621 gaatggctca ttctcggaag ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa
     1681 agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat
     1741 gcttgaacag atgacgaaaa aatacgacaa tatgacgctg attgattggt acagctacag
     1801 taatgaccat agtgactggt tttatgatga tcaagtccat ccaaatcctg aagggatgac
     1861 acattacatt cacttagtca gcgaagcctt gctaggagga agttcaagta cgaacagcag
     1921 tgagacaaac gtgacagaat gatagctaca aaaaacttca ggcaaaaaca tttgtttttg
     1981 cctgaagttt ttttatattt accgtgtttt taacattcat atagattcag gaagcaaaac
     2041 ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc
     2101 ttctgctttt gttgcttcgc ttcttgaaga aaatacttct gcatggatca tcttaacagg
     2161 tcttcttttt tgaagacgag tatatttagc accagtacca ttgttatgtt cctctaatcg
     2221 tctagaaaga tcggttgtat atccaccata aaatgtctga tctttacaga ataatacgta
     2281 aaaataatgt tcatttgcca tagagcatct cacgaatctc tgcagtgtat tctccatcca
     2341 tattgtaagt gatcaatggt ggagcgactt tgaatccgtc taatttgcct tctttgattc
     2401 cttcaactaa caaaatattt gcctcttttc caggttttgg gtaaacaaat tggatccttt
     2461 taggtgcaat tcgatagtct tccatgacct gaatgatttc taagaaccga tcaggacgat
     2521 ggaccatagt aaagcgtcca tttgttttta gtagcttact tgaaatatcg atcacttctt
     2581 ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg tttattaggg ttcgttttat
     2641 ttgttggcag ccctttgaaa taaggtggat tacagacgac taagtcacaa gaattatgtc
     2701 gaacgacaga taagctgtct ttcagatcga tggtgtggac agtcatttgt tcatttaaat
     2761 ggttaagcgc gatactacgt cttgccatgt ctgctagacg aggttgaagt tcgatagcgt
     2821 cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa
     2881 ggtcaacaat cttccctttt ttaggtacac gggaaaagtg agcaagcagc acagcatcta
     2941 aagaaaaaga aaacactttg gaactttgga taatcttgat atcttctgcg tataattggt
     3001 cgattcgttc gtcttcgtga agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt
     3061 attatactat ttttattaaa aagaaccaag gtggcttgtc aaacctctat tttttatgca
     3121 tactatgacc ataagaaggg aaaggaagag actatgtttt atcgatttat gcgcggggta
     3181 gttcgagtac tattagcctt agttaatgga aatgcccgct atgagaataa agaagtttta
     3241 ccgcaagatg agaattacat actagttgct ccccatcgta cttggtggga cccattatat
     3301 ctggcggtag ctgcagcacc caaggaattc agctttatgg caaaagaaga actgttcaaa
     3361 aatccagttc tgcgatttat attaactcga tccaatgctt ttccagttaa aagagaaaat
     3421 ccaggaccaa gcgtcattaa aacacctgta aagattttga aatcgacaaa tttgagtttg
     3481 atcatgtttc ccagtgggac aagacatgct acagaactta aaggcggaat ggcattgatt
     3541 tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc aaggtccgtt gacacttggg
     3601 gatctgttca agcgtcagag agtgacagtg cgtttcggcg aaccaatcga cttatcagat
     3661 atcaagaaaa cggataaaga aggtatggcg gagatcgaac gacgaacaca agcagctttt
     3721 gacaaattgg acaaagaagt gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat
     3781 gataagttta tgcgtgggcg gtcttttgaa tctactgtcc tgttcagttc agactttttc
     3841 aatgaaaacg agggagagac gtcaagtctg ccctcttttt gcgttccaat atgtaaaata
     3901 gaagacaggt gtcatgtaat ggatcaaagt gttataataa ttagaaggac attttttgag
     3961 gtgattgaat gcgtttttta catacagccg attggcatat cggaaaaaaa ctacaaggtt
     4021 atgacttgat tgaagaccag cgtcatgtac tatcttctat actagatatc gcgaaagaag
     4081 aaaaagtaga ggcaatcgtg attgccggag acttatatga taggagtgtc ccagctgttg
     4141 atgccgtaga attatacaat gaattgatgg tcgattggaa tttaaaggaa aagatcccga
     4201 tttttgctat atcaggaaat cacgatagca gtacgagact ttcagcgggt acgccatggt
     4261 tcagttattc gaactatttt ttgcgaacac agctagcaga agcatttgaa ccagtcgagt
     4321 ttaaaaacgt acaatttttc tttcttcctt actttgaacc aattgcagcg agattatatt
     4381 ttgaagatga caatatccga acgatcaagc aagcggtagc caaggtgata gataaaatgc
     4441 aggagctatt tgattcagaa aaaaaacaag tattgattag ccattttttt gtaatgggaa
     4501 gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg gttggattcg atccctggtg
     4561 aactacttac accgtttgat tatgtagcac taggacattt gcataatcaa gctgctttga
     4621 gacaggataa tgctcgctat agcggttccc cattgaaatt ctctttatct gagatcaacc
     4681 aaaaaaaagg cgtatggatc gttgacctga caacaggcct ggagttgaat tttagggaga
     4741 tcgaacccct gcatgatatt gaagaaataa cagcgagttt caaagaattg ctggatcctg
     4801 ccttttatca gtccgtcaat cgggagaatt acatctatat ctatctcact gatagaacga
     4861 tcatcccgaa tatgatgaac cagcttcggc aaatatatcc tcgtattttg gggattgaac
     4921 gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa agtactagaa atgaaagatc
     4981 ctcaaaaatt agcagaagca ttttttagtg aaatgacgga agagcagcta actgcaaacc
     5041 aagaaaaatg gctcggagaa acgttgcaag aaatcatgga aatggaatga tcaagaatga
     5101 aaccgaaaaa attacaactt aaaaattttg gtccttttat caatgagacc gtagattttt
     5161 cacgtttgac agaagcaccg ctttttttga tcagcgggaa gacaggagct ggcaaaacga
     5221 cgatttttga tgggatcacc ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg
     5281 gaaaagagat gcgttctttg tttgctacac cagacgaaga aacaagcgtg actttttctt
     5341 ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac
     5401 gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac gttgaccatt tttgatggga
     5461 aaggaaaaga aataaaacaa atcacaaaac gtacagaagc agatcagttg atcagagaac
     5521 tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt actaccccaa ggagaattcc
     5581 gtaatttttt ggtttcatca agcagtgata aagagactgt tttacgtcat ttattcggta
     5641 ctcaattttt ccaacagttc aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa
     5701 aattggatca tttagagcaa gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag
     5761 aagaggaacc agcagcaggg tttgaagccg ttttagccca atggacaaac catcaggcaa
     5821 tactcgaaaa acaaatcacg gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa
     5881 agcaactaga aaattcctac tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa
     5941 cgatattaat aaacaagcaa aaggaattag ctgctcaaaa agacgaaata gaggaaaaga
     6001 aacgatggat tgggtattat gaatttacgc aacgattagt cgaaccaatc cagcgcctac
     6061 aagaaataaa agttgaaaaa atagaactgt cacaaaaaag tagtgagcaa acaaagaaac
     6121 tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc
     6181 gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa caaacagatg atggtgccaa
     6241 ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca attaacaatt tcattggata
     6301 aagaagaatt gaatgccgag aagctgtatc aaaatctcgc tatctatcag gaaagaactc
     6361 gaattcttca agaaaattta ctggatcagg aaaaactgca agaagatcgt cttgcttttg
     6421 gacagttaaa atatctagat gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca
     6481 ctgggtcgct cgcacagata agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa
     6541 atagtcagct attgaaagaa cagacagaac aagcagaaca gctgaaaagc caatgggcaa
     6601 agcagcaaat tgctcgtctt cagctttttt tgcgtccagg ggagccatgt cttgtttgtg
     6661 gatcaacgga acaccccata caacaattaa aacacgaaca gccaaataag gaagatatct
     6721 taaaagtaga acaacaactg gcagaagctg agtctgctgt agaaagaact cagcaaatca
     6781 ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca attatcagat ttagaacaaa
     6841 aaatcatggc aacgaatata caattggctg aacaagaaga acacttgcaa caggctttga
     6901 aaaaggcatt tcctgagata acagaacaaa aaacaaaaac aactcttgta gtaatcgaac
     6961 aatctttaga tagacgaaaa agcaagatgg ccaatgaaag aatagagctg gaagaaataa
     7021 gacaacaagc agaaaagctc gaacaacaat tagctgtcta caaacagcag cagacagaaa
     7081 aaaataatca gctccagcaa ctgcaaggag aaatcaaaag tttagaacaa caattgcaag
     7141 atacggatag taactgttta gaagaggaga tacgacgact tgaaaaacag ttagaagaag
     7201 actcgaatca actagctgtt gagcagaaag caggagtgca attgttgcaa gatcttcata
     7261 cgcttcggac acagcttgat cttcaacatg aacaaataga aaaaatagat gaaaaaatag
     7321 aaaaggaaca agaaagaata gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg
     7381 aagaaatctg tgcatttgca caacagcaag cacaatataa agaaaaccaa cgtacagtta
     7441 cttcttatca tgaagagcaa ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc
     7501 agctaactga gacattccca gatacagaag agatcagcca acagttagaa gaaataagac
     7561 gtatgatcga aataaagcaa gaaaagatat acggtttaca agaacaaaaa attagtaatc
     7621 aaaaaatcta cgatgaatgt ctttcgattt atcacacaag ccagacgaaa ttagatgagc
     7681 ttacgcaatt acagcagctt tcgcaaacat tcaacggaga aaatcctaag aagactagtt
     7741 tggaacggta tgtgcttcag gtttatcttc aagaagtgtt acaagtagca aatgatcatc
     7801 tccaacgttt aacgaaaaat cgttatcaat ttgagctagc ggataccatt ggcagttatc
     7861 gagggaaaac cggattagaa atcaatatct atgatgatca ggcaggtatg tctcgaagtg
     7921 cgcataccct gtctggcggt gagagtttta ttgcagcact ttctttagca ttagctcttg
     7981 ccgatgtgat ccagacacaa gctggtggtg tagcaatcga tgcattgttt attgatgaag
     8041 gatttgggtc attagatgaa gaagctttgg aaatggcaat ggaagcttta gaaacgatcg
     8101 aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag agaattgaaa gaacgagtat
     8161 tacaacaaat aagaattgat acagaaggta gcggacaaag caaaatacgc tatcaacttg
     8221 gataaaaagg gagattggta actatgaaat ggagtattcc agaaaaagtc attgcacgtg
     8281 gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg
     8341 tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt tgatttggat ggtacagcaa
     8401 aagagataga ttactgccaa tgtccctatt gggaggaaca taaatattgt aagcatacag
     8461 ttgctgtgga actttattta cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc
     8521 caatgctaga accggtaaaa gcaacaagtg agggagaaat cattgcaaaa ggtttttctc
     8581 gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt gattattgaa taccaagtag
     8641 aaacgattga gactaatcag tatcacccag agctttctat tttggcactt tacttgcgta
     8701 tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa aaacatctat gattttttac
     8761 aagcttacac agaaaaagaa acttacactg ttaataaaca gtaccagttt cgtctagata
     8821 aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca tcttgcagga agtgctcaga
     8881 cgcatcaatt attaggtacg aacggcttac aagtcaaagg aaaactagat aaaagatatc
     8941 tgcttctccc agtagaacaa gcccgcttcc tgttagagca gatgaatcag actacacgtt
     9001 tgcatctgca gataaatgaa caaaacgatc aaggaataca attttctgat caaaacaaac
     9061 ctttatcatt caaagtggaa aaatttggag aagattattt gctgcaagca ttgaatgatt
     9121 ttgatttttt ctttatgcat tatcagtggg gagttatcca agggacattt tattctctga
     9181 cgaaatatca acaaacggtt tatcaaactt ggaaacaatt gattcgccga ttggagaaac
     9241 cagaagtagt gttcaagaaa aaagaattac cgtctttgtt taaagagatt atccctctgc
     9301 tgtcagagat cggcgacgta gctattgccc ctgcagtaga agaggaagta gcagatatcc
     9361 ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt
     9421 tcgtctatgg tgaagtcatc tattcgactg acccgaagca tgaggtctcg acagaacagg
     9481 cttttcaggt gttacgtgac acagtagaag agaaacgagt acttgatcta ttcagaatgt
     9541 atcgctacca gaaaaatgaa actggttatg aacgtccatt gccaagcggg gaagaactat
     9601 atgccttttt ccgcacagaa ctatctgtct tccgtcagtt tggtgaagtc agacttggaa
     9661 gaaaattgcg agagttgtat ttagatgcgc ataaacatca accgaaaatc gaagtaaccg
     9721 agaccggttc atggctcgat attcgattcg atgtgacagg gatagaggag caagaaatcg
     9781 atcatgtcct gcaaagcttg ctgagaaatg atgcttttta tacattagaa aatggtcagg
     9841 ttctttcttt tgattcagaa gaatttcaac aaacaagcca agtgttgcaa caattacggg
     9901 aatcgatacg tacagaagaa ggaacgatcc atgtacctaa aaatcaagga ttgatcattc
     9961 aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc gttccaaacg atggtacaag
    10021 atctcattca tccagaaaga taccaggcac agcttcccaa aggattgaat gcaacgatga
    10081 gagattatca aaagcaaggt tttcgatggt tgaagatgtt aggtcattat cagtttggcg
    10141 gtattttagc cgatgaaatg ggacttggga aaacactaca gacaattgcc tttttacttt
    10201 cggaaaaaga agaacggaaa agcttctctg ctttgatcgt agcgcctgcg agcttaattt
    10261 acaactggca ggcagaggtg agaaaattcg ccccttcttt aagtatccaa gtaatcaatg
    10321 ggaataaaaa agaacgtgaa gaattactgg ctaaagacac agatatccga gtgacttctt
    10381 atgccagctt aagacaggat ctagctaatt atcagtcgca aaaaattgat tatttgattc
    10441 ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac tgcacaagca ttgagagaat
    10501 tagctgtacc tcagcggttt gcactgagtg ggacaccaat cgagaataat ttagaagaat
    10561 tgtggtcatt gtttgcaacg attatgccag gatttttccc gactaaaaca aaattcaggg
    10621 aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt tattttacgg cgagataaac
    10681 aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa tctatattct gcacttacag
    10741 aagaacaaaa aactgtttat ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa
    10801 tggattctga agcattcaag aaaaaccgga tcgggatact tgctggtctg acaagactgc
    10861 gacaaatttg ctgcgatcct cgtctgttta ttgaagatta ccaaggcggt tccggcaagc
    10921 tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa caaacgaaga gttttgctgt
    10981 tttctcaatt tacagggatg ctgactatct tgcaagaaga gttagccgag ctagggatct
    11041 cgacatttta tttaagaggc agtacgaaac cacaagatcg gttaagtatg gtagacgcat
    11101 ttaatgctgg cgaaaaagat gtttttctga tttccttaaa ggctggggga actggattga
    11161 atctcacggg agcagatacg gtcatcttgt atgacctttg gtggaatcct gccattgaag
    11221 aacaagctgc tggacgtgca catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta
    11281 tgatctctga aggaacgatt gaggaacgca tggattcact ccagcaagaa aaacgcgaac
    11341 tgttccaaaa agtgatccaa ggtaatgaag aacagttgac gaaactgact gaagaagaca
    11401 tccgcatgat tttaagtgta ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt
    11461 aatgggtaag aaagcctttc taaagaagcg attgtttatt tgatttaaaa aatagcaagg
    11521 aggctgggat tccattttat cccagcttcc ttgctatcat ttttttattt ctatttttaa
    11581 aggttataca aagattgtct tcattgtctc taacagctga tcaaaagaag aaatctcgat
    11641 atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca
    11701 gttagcactt tttgcaccta gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc
    11761 ttctggaagc atatgaaatt ctttctcagc cagctgaaaa atctcttttt ctggtttttg
    11821 gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga atccaattgt tcaattgaag
    11881 ttgcttcagt ttttttgtct gatggtcagt cggcccattt gtgataattc ctaatgggac
    11941 attcttttcc ttcaagaaat cgagggtttt tttgacctcc tcatgcaagc agatattatc
    12001 aagttcttcc tcgtaaattt tttgaaataa gaggccatct tcttccgtaa tatgagggta
    12061 gtctaaatct tttagtgatt ggctgatccg atgtgctcgc atatattcta gtgtccaatc
    12121 tcctgccatt acttttggga agttttcatc actatgatgg cggaaccgga tatatagtgg
    12181 atgcatatct gcatctgaaa caagcggaac gactcttttg accgcgtttc gaaatggctg
    12241 ctgctgatca tacatcgtat catcaacatc aaacacaact gatttaatca aaattttaca
    12301 cgcctttcgg ttttcattga ttttttcaca aatccttgac tattgtaaca gaaaaaattg
    12361 tctaaaaaag ctgataaata aaggtttttc ttactatatt tctatttcct tgacagaata
    12421 aggtttttat atttcagttt ttttcactaa aaatcgtgaa acacgtcaat aagtt
//
LOCUS       DACDSY010000083        12279 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDSY010000083 DACDSY010000000
VERSION     DACDSY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.084"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            68..751
                     /locus_tag="IWK44_001890"
     CDS             68..751
                     /locus_tag="IWK44_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP9550393.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="IWK44_001891"
     CDS             753..1589
                     /locus_tag="IWK44_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9550394.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="IWK44_001892"
     CDS             1600..3354
                     /locus_tag="IWK44_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9550395.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="IWK44_001893"
     CDS             complement(3630..3971)
                     /locus_tag="IWK44_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9550396.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="IWK44_001894"
     CDS             complement(4039..6210)
                     /locus_tag="IWK44_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9550397.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6421..7278
                     /locus_tag="IWK44_001895"
     CDS             6421..7278
                     /locus_tag="IWK44_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9550398.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="IWK44_001896"
     CDS             complement(7349..8206)
                     /locus_tag="IWK44_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9550399.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="IWK44_001897"
     CDS             complement(8191..10893)
                     /locus_tag="IWK44_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9550400.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="IWK44_001898"
     CDS             complement(10906..12195)
                     /locus_tag="IWK44_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP9550401.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
BASE COUNT         3397 a         2614 c         2224 g         4044 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatct
//
LOCUS       DACDSY010000084        12229 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDSY010000084 DACDSY010000000
VERSION     DACDSY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.085"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            140..460
                     /locus_tag="IWK44_001899"
     CDS             140..460
                     /locus_tag="IWK44_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550402.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="IWK44_001900"
     CDS             912..1112
                     /locus_tag="IWK44_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9550403.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="IWK44_001901"
     CDS             1259..4048
                     /locus_tag="IWK44_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9550404.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="IWK44_001902"
     CDS             4095..4622
                     /locus_tag="IWK44_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9550405.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="IWK44_001903"
     CDS             4643..5833
                     /locus_tag="IWK44_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9550406.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="IWK44_001904"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="IWK44_001904"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP9550407.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8224..8898
                     /locus_tag="IWK44_001905"
     CDS             8224..8898
                     /locus_tag="IWK44_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9550408.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8895..9236
                     /locus_tag="IWK44_001906"
     CDS             8895..9236
                     /locus_tag="IWK44_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550409.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9266..9625
                     /locus_tag="IWK44_001907"
     CDS             9266..9625
                     /locus_tag="IWK44_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP9550410.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9828..10007
                     /locus_tag="IWK44_001908"
                     /pseudo
     CDS             9828..10007
                     /locus_tag="IWK44_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            10149..>12229
                     /gene="cadA"
                     /locus_tag="IWK44_001909"
     CDS             10149..>12229
                     /gene="cadA"
                     /locus_tag="IWK44_001909"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9550411.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKED"
BASE COUNT         4066 a         2138 c         2653 g         3372 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtggaaatg gctttccaca tttttgcata aatttggctt
     7381 aataccgaga aactggcttt tgaacaccgt ttaaggaaga ggttgtgcgc ctgccgttca
     7441 gctccgagtc acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga
     7501 cttgtgaccg aggagttggc cggagagcac cgtttattct aaaaaagaac tgcgaactct
     7561 cgcagttctt tttttaaaat acgggataaa ggaagtttcc agcagacaag agaagaaagc
     7621 aaaagaaatt gctatttgat tcacaaggtt ctataacgaa atcgcaatca atcaaacatt
     7681 tacaaagaag gtaattcaaa tggaaatctg atttgatgat gcttctgctg ttgaaataaa
     7741 gcagcatcta gcacctgaaa acaaactaat agtaacattc gaagatggag taggtccgta
     7801 ttttcagcat gcaataatcc atatgtaagt tcaattcacc cttgaaatca tcggaccaga
     7861 taaagttatg accaatctat cccaagcaat ctagaagaga tattagtaaa gggacattta
     7921 ctagaagtct tagatgagcc tataaatata tgttctgatg gcgtcatcag tggtctgatt
     7981 gacggaagct taggtgtgat cgatctctct ggaccaaaca acgaaggaat caaggggaat
     8041 ccggcacgtt aagaaaaaaa tagcataaaa aactagacac ttgatgagcg ttcattcaat
     8101 tgtttgaagg gactaggctg gtgtcatttt ttatacatat attttctctt tcgtcttgat
     8161 agggcttatg ggataatata aggaagaaaa tcatcgtttt attcaaaaag gagtttttga
     8221 caaatgaaaa aacgtatatt atttatcggt gatagcatta cagacgcagg aagagaccga
     8281 gcagaagcaa ctgatctagg taaaggttat ccacttttga ttgccggaaa actaatctcc
     8341 caatatccaa cgatggaatt tgaatgcatg aaccgtggga taggaggaga caaaattgca
     8401 gatctgcaag tacgctggca agaagattgt ttagctttac aacctgacta tgtatcgatt
     8461 ttgatcggaa tcaatgatac ctggcataat actagcgatc aggctatatt cggaactgag
     8521 aaatctgctc agcaatttga atccgtttat cgaaaattac taaatgaact tcaaacagtg
     8581 ggaatctgca acatcatctt gatggagccg tttgtacttc ctataaccga cgaccgtcat
     8641 ggctggcgta aagacttaga ccctaaaatc caaattgttc gtaaacttgc taaggaatat
     8701 ggcgcattgc tggttccttt agatggtata ttcaatgctt tagggattgt ttatggtttt
     8761 tcgaaatata caagagaaga tggggtccac ccaacgattg gcggacatga gatcattgca
     8821 caaagctgga tagatgcaat tgccgcagtc gattttttta gtggagataa aaatgagcga
     8881 aagaaaagtg agaaatgatg gaaatacggt gggcagtaaa agaagatcta cctcaaatca
     8941 atcagctgat tactgatcat ccagaatcat tcgtaaaagc aaaaacggca gctgacctaa
     9001 atgataagaa taaactattg ctcgttgctg taacaaaaga aaaacggatt atcggatttg
     9061 cgcaagcgga cgtgcaacag tcagaagaac gattggatca gcgaccatca ggaaaactaa
     9121 tccaacacac attagtaaca aaagaaaata cggaagaaat ccaaaaaaaa tttgcagaat
     9181 atttgatact ggaagctaaa aaaagaagaa taaaatcatt ttttgcccag ttttaatcaa
     9241 aaacagtaat agaaggagaa atacaatgag ttgtttagga aatgtcatct ggtttatatt
     9301 cggcggacta atcggtggtc ttagctggtt agcagcgggg atactttggt gtatcacgat
     9361 cataggtatc ccaatcggta tgcaatgttt taagatcgcc tccctttctt ttgcgccttt
     9421 tggcaaagaa gtaatttatc accagtctgg catgtcattg attgttaata tcatctggct
     9481 gatcgtttca ggcctaccaa ttgctctcgc acatgtcgtc agcggcatcc ttctatgtat
     9541 cacgatcata gggatacctt ttgccaagca atctttcaaa cttgctcgat tggcgttgat
     9601 gccattcggt gcagaaattg tataaaagag aataataaag gttagataaa gcatagagtc
     9661 aggtgggata catgtatttc acctgactct atgcgttctc ctgtataagt tcttccatgt
     9721 gacttttacg tcttttattt atatggggtt actgtttatg gaaagttgaa ataattttca
     9781 ttaaagaact ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac
     9841 attttgcagg gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat
     9901 tgccagcaac aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg
     9961 gttggataag agcagtggca atgatcgaaa agaaaaagaa agaataagcc aaaacatctg
    10021 tatggaagaa attttccgat gcagatgttt ttcatttttt ctttcggata ccttgacata
    10081 tgaataaccg ttcatttata attagttatg aagagatatt catatgttga ggcgaggagg
    10141 tcattaaaat gaaaagttac agattagaag gtttagattg tgcgaactgt gcaatgaaaa
    10201 tcgaaaaagg agttcagaag atcaatggag tcaaggaagc aacggtaaac ttcacttctg
    10261 gaaaactaac gattgacgca gaggaagatc atttggctac gattgaacaa gaaataaaaa
    10321 aagtagtgaa ggaattagaa ccagatgtca aagtaacaga aatagataag gaaaaagtat
    10381 ctgaacatgg aaatgaaaaa gagagaaata cgctgttccg gattttattt tcccttgcag
    10441 gtatcgcttt attgctatta tttgatttta atgaaccaat tcgcttgata gggtatttat
    10501 tgatttatct gctgatagga tatgatgtag tgaagaaggc agtgatgaat atcgtcaaag
    10561 gaaaaatctt tgatgagaat tttcttatgt ctgttgctac gattggcgct atgttgatcg
    10621 gtgaatatcc agaagccgta gcagtgatgc tattttatca aatcggcgag tatttccaag
    10681 gattggcagt cagtcattca agaaaatcga ttcgcgaatt gatggcgatc cgtccagaaa
    10741 cagctcacgt gcagacgacg gaaggattga tgaccgtgaa ccctgaagac gtgcagatcg
    10801 gtcagtttgt acttgtgaag cctggtgaac gtgtaccgtt agatggaacg atcatcgaag
    10861 gagaatcatt agtcgatacg tcagcactta caggagaatc agtccctaaa tctgtatatg
    10921 ttggtgaaac agtattaagt ggatttatca ataaaaataa accattttta gttcaagtag
    10981 aaaaatctta tgaaaattct actatcagca aattattaga attagtagaa aatgccagca
    11041 gtaaaaaagc accagctgaa aatttcatta ctaaattcgc gcgctattac acaccagtcg
    11101 ttgtaggatt ggccgtttta ttagctgttt tacctccact tgttgtttct ggagcggcgt
    11161 tttctgaatg gatctatcga gcattgacat ttttagtgat ctcatgtcca tgtgcattag
    11221 tgatctccgt accccttagt ttctttggcg gtatcggagg agcaagtaaa atcggtgtat
    11281 tagtaaaagg ttcaaattac ttggagttgc ttgcacaaac tgagacagtg gtattcgata
    11341 aaaccggcac attaacgaaa ggtgactttt cgatccagac gatcgataca aaaatcgatc
    11401 caaagatatt catgcaatat gttgcgagtg cagaacagtt ttctacacat ccgattgctc
    11461 aatctgtttt agaaggctac gcaggtccgc ttttaccaac agctaatatc caagaatttg
    11521 caggtgaagg gatcttagct gaagttgatg gcaaacaagt attagcaggt aatcacaaat
    11581 tgatggaaag atttgaaatc tcttttcctt caagtcaaga aatcggtaca ttgctttatc
    11641 tagcgatcga tcaaagttac agtggttatc ttgttattgc agataccttg aaagaagatg
    11701 ccgtagacgc attggttcaa ttaaaaaagg ctggtgtgaa gaacacagtc atgttgacgg
    11761 gcgattcgaa aaaaattgct gatcatatcg ggaagcaagt aggcgtggac aagatatata
    11821 gcgaattgct tccagaagat aaagtgcagc ggttagagga aatcttacaa agaaacaata
    11881 aaaagaccgc atttgtcgga gatggaatca acgatgcacc ggtgctagca agagcagatg
    11941 taggaatcgc aatgggtgga ttgggaagcg atgctgcaat tgaagcagca gatgtagtca
    12001 tcatgaatga tcaaccatcg aagattgcag aagcaatcca tttagcaaag aaaacattga
    12061 aaatcgtcaa acaaaacatt gtctttgcta ttgggataaa aattttggta cttgccttag
    12121 gtgcttttgg ttttgcatca atgggagatg cagtatttgc agatgttggg gtgacagtat
    12181 tagctgtttt gaatgccatg cgaagcttgc atgtaaaaaa agaggacga
//
LOCUS       DACDSY010000085        12200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDSY010000085 DACDSY010000000
VERSION     DACDSY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.086"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            190..1128
                     /locus_tag="IWK44_001910"
     CDS             190..1128
                     /locus_tag="IWK44_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9550412.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            1140..2051
                     /locus_tag="IWK44_001911"
     CDS             1140..2051
                     /locus_tag="IWK44_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9550413.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            2075..3547
                     /locus_tag="IWK44_001912"
     CDS             2075..3547
                     /locus_tag="IWK44_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9550414.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            3598..4209
                     /locus_tag="IWK44_001913"
     CDS             3598..4209
                     /locus_tag="IWK44_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9550415.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            4239..6152
                     /locus_tag="IWK44_001914"
     CDS             4239..6152
                     /locus_tag="IWK44_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP9550416.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            6280..9261
                     /locus_tag="IWK44_001915"
     CDS             6280..9261
                     /locus_tag="IWK44_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP9550417.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            9258..10919
                     /locus_tag="IWK44_001916"
     CDS             9258..10919
                     /locus_tag="IWK44_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP9550418.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            10959..11891
                     /locus_tag="IWK44_001917"
     CDS             10959..11891
                     /locus_tag="IWK44_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP9550419.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4006 a         1930 c         2521 g         3743 t
ORIGIN      
        1 attatagcac atattacaag tgatgtaaac gctttgcaaa cagggctact tatgattgtg
       61 aatacagttt ccgtttaaaa actattctat tgtagaggtt taataaacta aaaataatgt
      121 ataaaaaatt aatgtaagcg attaaccaag tgggtataat caaggaaacg ctgagagagg
      181 agagacaaaa tgaagctaaa gaaagggtta ttaggaaatc taaaaaaaaa ccgagcattg
      241 tttattatgc ttttaccagg tgcgttatgg ttatttatat tttgctatat accaatgttt
      301 ggagttgtaa tcgcttttaa agatttcaga atttcatcaa ggggattctt tacaagtttg
      361 ttcaacagtc cttgggttgg ctgggaaaat ttcaaatatc tctttgcttc gcaagatgct
      421 tggatcatta ctcgcaatac ggttgtttat aacgttgtag gtattttgct aagtactttt
      481 tgtacagtgt cattagcaat cattctgagc caaatggcga acaaacggtt attgaaaaag
      541 atgcagacat ttatgatttt tcctaatttt ttatcatggg tagtcatcag ttactttgtt
      601 tacgcatttt tgagtacgga taaaggatta attaatattt tactaagcaa ttttggtgtg
      661 gaacccataa attggtatag tgaaccgaag tactggcctt ttattattgt ttttacgagt
      721 ttatggaaag gcgtaggcta tggatccatc atttattacg cgtcaattat ggggatcgat
      781 actagttatt atgaagctgc tttagttgat ggtgccagta aatggcaaca gataaggaat
      841 gtagtcttgc cacatttaag atcgattatt gtcattatgt tgattttgtc tgttggtagt
      901 atttttcgag ctgattttgg attgttttat caattgccaa gaaattcggg ggctctttat
      961 gatgtgacga atgtattaga tacttatgtc taccgtggat taatgaatag cggagatatc
     1021 ggaatgtcta cggcagcggg attatatcag tcatcagttg gctttgtctt gattttgatt
     1081 actaataaga tagtcaacaa gttagatcct agttcagcac tattttagga ggaatcaaaa
     1141 tgatatcaaa attaaaacgg cagatgtact tttcgctgcc agtcgaacgg tttttcacaa
     1201 tattactaat cttatttact cttagctgta tttttccctt tattttcgta atcgtcattt
     1261 cattaacaga tgaaaaagca ttacaactca acggttacga attattgccg gctcaatgga
     1321 gcactgatgc atatcaatac ttaatacaag atggcggaca gttacttcgg tcattaggcg
     1381 ttacaattat gattacagtg attggaacgt tgattactgt ttttatgacg ggaacctatg
     1441 cttatgtttt atcaagagcc tctttccctt accggaagtt ctttactttt tatctttttt
     1501 ttaccatgtt atttgcggga gggatggttc cttcttactt agtgatgacg aagatgcttg
     1561 gcttaaaaaa tacaatttgg gctttgattc tacctcttgc ttttagccca tacaatgtca
     1621 ttattttaag gacattcttt aagaagtcga ttccagaatc gattatcgaa tccgctaaaa
     1681 tggatggctg tagtgagttt cgagtttttt tccaaatcgt tttgccttta gcgatcccag
     1741 gggttgcaac aataggttta ttttcaagct taggttattg gaatgactgg ttcaacgcat
     1801 tgctgtatat tgacaccaac aaattgattc cattgcaata tttgttaatg aagattcaaa
     1861 attcaatgga attcttagca aataataatg acattacatt agcgcagcaa caagcaattc
     1921 aaaatagcct tcctcaagaa tcaacacgaa tggcaatggt cgtagtagct actttaccga
     1981 ttgcaattgt ttatccgttc ttccaaaaat actttgttca aggattgacg atcggtggag
     2041 tgaaagaata aaaaagaata ggagatgtta aaagatgaaa aagaacatgc agaaatattt
     2101 gattggtggt gcttctgtac taacccttgg cctattaggt gcttgtggta gcagtaatgg
     2161 cgaagcaaat aatgaaacag gaaccacaaa agttacttgg tatcaggttg gagaagaacc
     2221 caaagatttt gatgaggtca tgacaaaagt caacgaaaaa ttaaaggaag aaatcaatgt
     2281 agagttggat atgcggctga ttccggacgg ggattatcag caaaagcttg gtgtgatgat
     2341 caattcagga gaagaatatg atatttgttt cgtcaatggc acagattatg tgaattatgg
     2401 gaacaaaggt gctttcatta gtctcaacga tcatttggat aaagagttga aagattatgc
     2461 agctgaattg aatgaaggat ttattgaagg tggggcaatt aatggcgaaa cttatggatt
     2521 gccagtcaat aacgatattg ccacaacgga atattggacc tttaataaag atttggtaga
     2581 tcaataccaa ttggatgtag agaatgttga aggtgtagaa acattggctc cagtattaga
     2641 aaagtttgca gaaaaaaata ctgatacgaa tattacgcca atcggtatga cccgtaccag
     2701 tgtctgtatt ccttatgaca atattatcgg tggtggtgta ccgctagcgg ttgcatttga
     2761 aggcgataca caaacagttg ttaatactta tgagactcca gaaatgatga cttatttgaa
     2821 ggctatgcat gagttttata aaaaaggata tattttgaaa gatgcagcca ctgttacgac
     2881 agatccattt cgaattgata gtaaaaactg gtttttaggt cgtaactcaa gtggttatca
     2941 accattagca caaaaaaaat tagaagctct agcgggacat ggattaattt taaaaccagc
     3001 tggaaaagct tttaaaacga ctggatttgc aagagtggca atgcatgcgg tatctgcttc
     3061 aagtaaacat ccggaagcag ccttgaaatt gttgaattat gtgaatacca atcaagaagt
     3121 agcggattta atggtttatg gaattgaagg aaaacactat gatcgcaatg aagatggttc
     3181 gattacttta acaaactctg atgattatta tcaaggcgct tgggcattgg taaatactta
     3241 tctagtttca aaaacttcac cgattacaga agaaagtaaa aaactagaag aagaaagtaa
     3301 aaagtttcaa aatgaagcac cgatgtctcc tattttaggt ttctcttttg acgcttctga
     3361 ttttcgaaca gaaattgcaa gtgtacaaaa tatcgtgtca caatatgaag tacttttaag
     3421 ctcgggaaca gttgaccctg aacaatatgt accagagttg ttagaaaaac tagaaaaagc
     3481 tggattggat aaaattcagg catctgctca agaacaatac gatgaatggc gaaaatctaa
     3541 caaataattt tttactaaat gaaaagaata gactaagcag ttagtggggg gattcaaatg
     3601 aacgaaaaca aatggaccaa aggatttaat tgggtttttc aattgttgtt actaaacttt
     3661 acttatttta tactaatttg tttaggattt ggaattttag gttttttccc tgctacgata
     3721 gctttgattg ctggcgttgt gaatcgaaaa aaatatggct tgcaagagtc tgtgaagttc
     3781 atgagtaaaa tctataagaa aaattttgtt agatataatc tatattgctg gtcagcaact
     3841 ttgttgttgg ccggcaatat tactacgatc tggctttgtt ggcagcaaag cgcaccattg
     3901 ttttatttat taagtacatt attatcggta attacactgt tcctagtctt tgcaatgttg
     3961 aattgggtcg tgatcaaaag tcttgggttg tggatgatgg gagaccgact gattgactat
     4021 ttgcttttca cagcaacgat gttgccaacg gtagtcatgc aaggcttggt gcttggaatt
     4081 tatttccgaa tattgatgat tttgcctgtg atttttctgt ttggcggaac agctgtaacg
     4141 gtaatattga ttagtaaatt aactgaaaga gcgttagcaa agttgccttg tcagatacaa
     4201 tcgcactaat tttttcacaa aaaggagtat ttctttctat gaagttaact gataaacaag
     4261 tagctaatat agcctttatc cagcaacttg agattccatt gacacaaaaa gatgaagaac
     4321 aaatagactt gatttttact gacacaaagg ctaatgtgcg acaaatcaag attactaaaa
     4381 atgagcagca acgaatttgc ttgtcttatt cacatgaaga cttgctgtat cgaggattgt
     4441 ttatctgcct tccacgtttt ttagctcaag gaggagagat gccatacatt gaagaaacct
     4501 cgatcattga gaccagttat tcattagatg cttctcgtaa tggcgtgatg aataaagaga
     4561 ctctaaaaaa attcatttct tacttagcgt tgatggggta caacacattg tatatttaca
     4621 cagaagatac ctatgaagta gaagaccttc catattttgg ttaccttcgc ggagcgtata
     4681 caaaagaaga agtgaatgaa attgttgaat ttgcttctgg ttttggcata gaagtagttc
     4741 catcgatcca gacccttgct cacttgaccc aatttttgaa gtggtatgct caacaagaac
     4801 tgatggatga tcaaaatact ctactgatcg gtgaagaaaa ggtttatgcg gcaatcgaaa
     4861 agatgattat tgcctgcaaa gaaatgtatc agacagagcg tattcatttg ggaatggatg
     4921 aagcctatca agcaggtctg ggacgatatt taagcctgta tgggtattgt gatcggacaa
     4981 ctttaatgat tcaacatatg gaccgtgtgt tatcaattgt tgataaatat aagatgaaac
     5041 caatgatttg gagtgatttc atttataagc tattagatga aaccggtaaa gataggtatt
     5101 acaatcctga ttttaaactc aaagcattag cagctaaagg attaccagca ggattaacct
     5161 atgttcattg ggactatggt tcagatgaag tttcctccta tgagaaagta attgataacc
     5221 atttgaaatt ttgtgacatt gatcattacg ttttagctag tggtgcccat atctggggga
     5281 gactagcccc gaatcatggg aaaacagaag caattgtttc tgcagctgta aaagcttcta
     5341 aaaataaagg tgtacgttct gtgatgttaa ctacttgggg agatgaaggg caggaaacag
     5401 agcattggca ttccttaatt ggggcattaa aattagcaga ttatgtctat tctgaagaag
     5461 agtggaatga ttttgaggaa atctacgatc gtttattagg aacaggtagt tatcaattaa
     5521 tgtccgaatt acgatatttc gatgaagtgg ataccattac aaataacaac ttacaaatgg
     5581 cttccgtgag taaactgcta ctttggcaag atccgttagt tggtatatat gactttcatg
     5641 tagccgctca taacaaaatt gccactagaa caattggtac ttattatcat gagttagctc
     5701 agaaattaga tatgaataaa aattggaatc caaaacaacc aattttaaca actattcgtc
     5761 aacgatatat acttttagct gaaattttag cacaaaaggc agaactaggc atctatttac
     5821 aacactgctg tctggtagaa gaccttgcag gattaatgga attagcgaat cggtgtgaag
     5881 cactagccaa tcgagtaaat gaattggcat ctattcatga aataatttgg tatcaaacat
     5941 acaaaaccta tggttgggaa gtattagaac aacgatatgc tgcattagct catcgtctga
     6001 aaaccgttgc gcgtcgtatt aaaaatcatc ttcaggggca agatgcccta gaggagcttt
     6061 tggaagaacg attaccgttt tgtcaatatc aagtgccgct tgaaatttct ggctttaatt
     6121 atcggcaaag ctcggtgtca ggctacaatt aacgaaggaa tcttcccgtt gaacggacca
     6181 cttgctcatc taatgggaaa gtggttcgtt ttctattcat aagtagctta ccactaactc
     6241 ttttattaag aagagaaaaa aagtaggaag tgagaaaata tgttatcgga ctattatcaa
     6301 aatctgtcag ttataagtgt caatacagaa ccattacgta attattatat tccttatgca
     6361 agtaaagaag aagcaaaaca agagttagaa agggaaaaaa cgagccagtt tatcgatctc
     6421 aatggtacat gggattttca gtattatcct tctgtccaac agttaccgga aaattttttt
     6481 caagaggcct ttactgaaag ctatgataga atttctgttc ctggagtttg gcaaacagag
     6541 ggatttgact tacctcagta tatcaatgta tcttatccaa ttccttttga tccgccttat
     6601 gtacctaatg ataccccttg tggtgtatat cggcgtcagt ttcaaattga gcaaacagat
     6661 gcacgctttt acttaaatct agaaggagca gatagtaatt gttttgtttg gataaataga
     6721 aactttgtag gctatgacca agtgagtcat agtacatctg aatttgatgt gaccaagtat
     6781 ttacaggttg gggttaatga aattcaaatt ctagtgatga agtggtgtga tggtacctac
     6841 tttgaagatc aggataagtg gcgaatgagc ggtctatttc gtccagtgta tcttcttcaa
     6901 cgaccaacta tgcatctgac aagttatcaa ctacggacgt tgactgatcc taaaaaagga
     6961 agcggaacat taatggtttc ttttcaacga tttactccta ttgaagtatc gttacctatt
     7021 tcttacgaat tattccatcc tgatggacgt ctcatagcga aaggggagtg ttactcggaa
     7081 caacaaataa atttggatgt tgaagattgc cttttttgga cagcagaaac gcctaaatta
     7141 tatacattac ttttaacatg taatggtgaa tattttaaag aaaaagttgg ctttcgagca
     7201 ctgacgatca agaatcgtaa gttattgctg aacggaacac ctattaaatt atatggagtc
     7261 aactatcatg acgtgaatca gcaaggcacg ccagtgatga cccgagagca gttacgtgca
     7321 gatttgttat taatgaaaaa attaaatgtg aatactgtac gaacggcaca ttaccctaaa
     7381 tcaccagatt tttatgaatt ggcggatgag attggtctgt atgtaatcaa tgaagcagat
     7441 ttggagtgtc atggtgtggt taatctatat gatactgaag cgaattatca actaattgct
     7501 aatgatccga cattcgaaaa gccgattatg gaccgagtga tccgttctat tcttccttat
     7561 caaaatcgaa gctctatcat tatgtggtct atggggaatg aatctggtta tggagtaaat
     7621 tttgaaaaag ctcaagcttt agcacggaaa ttggatgatt cacggttgat tcataatgaa
     7681 cgctcgattg aaccagttat tgaccaagtg aatgatttta gtaacttgga tgttattagt
     7741 caaatgtatc ccccactgga agatgttcat cgtcattgtc aaagaagtga tactgaaaaa
     7801 ccttttttta tgtgtgaata tagccacgct atgggcaacg gtcctggaga tcttaaaacg
     7861 tacttcgatt tgattcaaca gtatgattcc tttcttggcg gttgtgtctg ggagtggact
     7921 gatcatgcaa tcaatagagg aactttagaa acgccatctt atttatacgg tggtgatttt
     7981 ggtgaaaaat atcacgacgg caatttttgc gtagatggtt tattgacccc agatcggcaa
     8041 ttaacagtag gtgcattgga gtaccgtaat attcattcgc ctattcaact gattgctgta
     8101 gatgaggagc tgcaagaagt aaccttgagc aatataactg cttttacagc cggttcagca
     8161 tttaaccttt ctcttgaatg gtatatagat ggaaaaaaaa cagtttatga agtaatgaat
     8221 cctttcatac ttgagcctgg caaaagttgt tcattgccca tcacagtacc tacaacgatt
     8281 catggtgctc atacggttgc tgtttcgcta tctgtttata aaaatgatga gctttatgga
     8341 cagcaacagc tctttcttaa aaaagaaaaa atgagattgc caagaactgc tcatcataat
     8401 gatgcaccac aaatttcagt aaaaggcaca gagctgataa tcaaaggaga atcctttatt
     8461 tatatatatc aattagcaaa agctgcgttc acttctgtaa agatcaatca aaaggaactt
     8521 ttggaacgtc ctactcaatt ttcaatttgg cgtgcaccaa cggataatga tcgtaactta
     8581 gtaaacaaat ggaaagcagc tggatatgct gaaatgtctt tagaaatcag aaaaaaagac
     8641 tggaaattcg tagatgatca tctcctgatc gaaacgtctt atcgaggagt tattccagga
     8701 ttacagccaa tttttacaat gcacatacag ttgaaaataa atggtgcagg cgtaatcaac
     8761 caaaaagtat cagtggagaa aaatccgatt tttccagatt tccctcgctt tgggatggac
     8821 tggccgttga atgatacttt ttcgaaggtt tcttattgtg gctatgggcc gtatgaaagt
     8881 tatcctgata agcactatgc tgcgatgttt ggtatttatg agatggagat tgaggaattt
     8941 gatcagccat atatcaaacc acaagaatat ggtaaccgct ggcaatgtga ttgggtatca
     9001 atcaaaaatc atcaaggatc taccatgacg gtttatggaa actctaattt ttctgctctg
     9061 ccttatagtc aagctcaatt aaccgaaaag tcacaccatt atgaattaat aaaagaaaat
     9121 agaacttggt tcaatttaag ttataaacaa aatggtatcg gctcacacag ttgcggtcct
     9181 tatttagaag atcgtttccg ttttatggaa gatacttttg actggaatat agtgattact
     9241 ttcaaaggag aaaatagatg aaagaaacaa agatcaatca agtcaaaaaa aaattatctc
     9301 gttatcaaga agttgtttat cgaaaccatt gcttaaaatg gtcaaaaaat aagctgaatg
     9361 ttcgttttaa tggattattt tatcgtttcg aacagttaga agaagtctgg aaaccggtaa
     9421 cgaatttcca atcatgtcct gtacagctca taaatgatcc aaaagaaaca tacgatttat
     9481 gtataacaca cctttataac cagcatttga aaaatgacgg attttattta aaggttacta
     9541 atgacaatca acttacgatt gaatcagcaa accttcgagg gtttcaatat gctatggaag
     9601 ccttattaga gagttttttt tcggtaggag atcaattatt attaccggaa ttgattctga
     9661 aacatgaacc atcagtgaaa attcgaggaa tcattgaagg gttttatggt atcccctgga
     9721 cacatgaaat gcgcctctct cttttttcat atatgaaaga taaccaattg aatactttta
     9781 tgtatgcccc taaagatgat gaattattac gtaaaaaatg gcgtgaactc tacgatgcgc
     9841 aagaattaga caaatttaag gagcttttat ctgctgctga ggctagcaat atcgattttt
     9901 ggtacttaat tagtcctgga aatgatattg atattacgtg tgaagaagac attcaagttc
     9961 ttctgaaaaa gttagaacaa ctgattgagc ttggcgtatt tcaatttggt ctattaatgg
    10021 atgatattga ctatcaatta aaaggtgctg ctaaacgccg ttttcgagag ccagcattcg
    10081 ctcatgccta tttagtgaat cgagtagagg aatatcttag cactcggttg tccaatcatg
    10141 aattagtcat ttgtcctaca gaatatgata atcgtcatgg ttctcgctat ttagcaacgt
    10201 taagtcaaga aataccagaa caattaccgt tcttttggac tggcccaagc acgttagcgg
    10261 cgtcaatttc tacagaagag ctgcaggaaa tggcttctgt ctatcaacgg ccgatgctga
    10321 tttgggataa tattccggta aatgattatt tagaggataa agaactattg ttcatgagtc
    10381 catatgagaa tcgaacaccg aatctttcta aggagagata ccaggttaca ggagtagttt
    10441 cgaatccaat ggctcaatta gaagcttcaa agtttacgat caatagtatg gctaattact
    10501 tatggaactg tgagcgtttt gatcctttgg aaacttggac gtcagtcgtt gaaaaagtag
    10561 ttggaagtaa actacaacca gcttatctta ccttaaccaa tgcttttcca aatcattaca
    10621 caagcagtct gctttcagat gaagaattac tgctagccaa agacccagaa tgggtcacaa
    10681 aagaaatggc tgctttagtc caagcagtaa aatggatcca gcaagaacaa catacatcta
    10741 gattgagaac agaattggag ccttggttac aagcaatcac agaaagttgg actttctggc
    10801 aagaatggca attaaaaaaa gatccaaaag aagcagaaga atggttggct aagaaaaaaa
    10861 cacaccgcat aggacgtgat ttagtaactg cccatttaga gcaaattatc aaaagttaga
    10921 tatcttaaaa aataaaaagc aagaaaaagg agaacaaaat ggtcaagaga ttaattagtg
    10981 caaactacag tgatgtcaag aaaatgacgt caaaagagtt gaagcaatcc atcaaagcga
    11041 gtgaaggacg ggtgattgtt tgtgaaacgg ttgctaatac tcttccacaa tctggagaaa
    11101 ttacgaatgc agaagttgcg tcagcatttg gtgcagattt gattctttta aatggtttcg
    11161 attgttatcg tcctgcagtt tttggactac caaatgtgac gattgagaag gtttttgatc
    11221 tacagcaaac gattgaaaac ccgattccta aattaaaaga actagtcggt cgtcctgtgg
    11281 gaattaattt agaacccatt gcccctaaaa caaatatgat gagtgagaaa gtagaaatca
    11341 gccaaggaag agcaagctct gttgaaacga tacaaaaagc agaagaacta ggagtcgatt
    11401 ttatttgctt taccggtaat ccaggcactg gagtaaccaa tttagaaatt actaaatccg
    11461 taaaaatggc aaaaaagtat ttttcgggat tgattattgc tgggaaaatg catagcgccg
    11521 gctcagaaga gccagttgtg aataaagagg cgattatttc ttatgcggaa gcaggcgcag
    11581 atattttact agttccttca gtagggtcta tcccaggctt tttagagcaa gacctaattg
    11641 aagcggttcg gttggcaaaa aaatatgatt tgttaacgat gtctgcaatc ggtactagtc
    11701 aggaaagtgc ccgaccagaa acattgcggc aattagccat tatgaataaa agttgtggtg
    11761 ttgatttaca acatattgga gatgctggat acgggggatt ggcaccagca gaaaatattt
    11821 tcgaaacctc ggttgcaatt agaggaatgc gacataccat caactgcgtt agccgctcga
    11881 tcaatcgcta acaaaattaa ataaaaaatg gttaaattga ttatttaagt gggaacatac
    11941 ttgagatatc agctaacttc tgtcacttta aaaaagggac tgtgacagaa gtgaaaatag
    12001 acaaaaaata tccgaacaag agactaggat agcagaaaaa ctatcgtttt gcaacgagga
    12061 ccaactggac cgtaggagca attctcttat tgttcggata tttcgcttgt tcaaggactt
    12121 atgtctgtag tgcccttttt tggtaggaaa aatggtaggg aaccatatca tttttaatga
    12181 aaatcaagca tttcaaaagt
//
LOCUS       DACDSY010000086        12111 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDSY010000086 DACDSY010000000
VERSION     DACDSY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.087"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            31..1317
                     /gene="efmA"
                     /locus_tag="IWK44_001918"
     CDS             31..1317
                     /gene="efmA"
                     /locus_tag="IWK44_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP9550420.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1455..2054)
                     /locus_tag="IWK44_001919"
     CDS             complement(1455..2054)
                     /locus_tag="IWK44_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP9550421.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2458..3579
                     /gene="mnmA"
                     /locus_tag="IWK44_001920"
     CDS             2458..3579
                     /gene="mnmA"
                     /locus_tag="IWK44_001920"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP9550422.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            3825..5246
                     /gene="arcD"
                     /locus_tag="IWK44_001921"
     CDS             3825..5246
                     /gene="arcD"
                     /locus_tag="IWK44_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP9550423.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5260..6378
                     /locus_tag="IWK44_001922"
     CDS             5260..6378
                     /locus_tag="IWK44_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP9550424.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6438..7730)
                     /locus_tag="IWK44_001923"
     CDS             complement(6438..7730)
                     /locus_tag="IWK44_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9550425.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            7880..8440
                     /locus_tag="IWK44_001924"
     CDS             7880..8440
                     /locus_tag="IWK44_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9550426.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8569..8946
                     /locus_tag="IWK44_001925"
     CDS             8569..8946
                     /locus_tag="IWK44_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP9550427.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            8879..10951
                     /locus_tag="IWK44_001926"
     CDS             8879..10951
                     /locus_tag="IWK44_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9550428.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11162..12109)
                     /locus_tag="IWK44_001927"
     CDS             complement(11162..12109)
                     /locus_tag="IWK44_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9550429.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
BASE COUNT         3892 a         2220 c         2593 g         3406 t
ORIGIN      
        1 tacaaaatga aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg
       61 aattggaaga gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt
      121 atggtcgtcc aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt
      181 ttgagttttg ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga
      241 ccgctagtgg atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg
      301 attttcgcgc ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta
      361 gtttttgtgt ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag
      421 tcgattatgc cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga
      481 atggtccaat cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc
      541 gttcctgtca attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta
      601 ttgatttttg tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt
      661 tttaaagatg caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata
      721 acgatcaatg gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg
      781 atgacgctag attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac
      841 gcagtcggaa tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga
      901 atgaaaccta ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg
      961 gttccaaacg atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt
     1021 gcaacgccat atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat
     1081 gttttaggtc gtgtcttagg gaacttcaac tctttgatga acctgacagg tccaattgga
     1141 ttattatttg caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc
     1201 ggaatcggga ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat
     1261 gataaagaat tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat
     1321 ggaatggagt tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg
     1381 gcaataagcc tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat
     1441 atagggcagt tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt
     1501 tgttgctcag ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt
     1561 gcgtctacgc ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca
     1621 tgaagattgc ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat
     1681 tgttgatggt cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg
     1741 tcagtaagcc taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc
     1801 cctttgatca agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt
     1861 gctttttcat attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct
     1921 tttattactg cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag
     1981 agccgcggag caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg
     2041 ataaagtaat tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat
     2101 ctgagcaact cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg
     2161 ataagaagca attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga
     2221 aaggctggcc ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg
     2281 attttttggt tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac
     2341 gacttgataa ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa
     2401 agttgcgacc ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg
     2461 acagacaaca gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta
     2521 acagcacttt tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg
     2581 gatgacacgg acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa
     2641 gtagctgctc aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat
     2701 cgtgtatttg aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg
     2761 atgtgcaaca aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc
     2821 gattatgtag ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac
     2881 atgcttcgtg gagtagacaa taataaagat caaacttact tccttagcca attatcacaa
     2941 gaacagcttt caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca
     3001 attgctgaac gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt
     3061 attggtgaaa agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat
     3121 atggtcactc ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt
     3181 ggtcaacgtc aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt
     3241 gtaggaaaag atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca
     3301 ttgtatgcca caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca
     3361 aaagaattca agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt
     3421 cgcttattgg atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact
     3481 cctggacaag cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac
     3541 cacgcatatc aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa
     3601 cagctatggc taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa
     3661 taaaattaat ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc
     3721 aataatatga ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc
     3781 ttttgcaagt gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga
     3841 cgggtattag caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg
     3901 tgtttgggat cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat
     3961 ggataatcgt tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa
     4021 agcgtccaga tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag
     4081 gtggcttcat cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg
     4141 caacgatgct gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg
     4201 ttccttctat tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg
     4261 gagttgaagg agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat
     4321 tcctattttt aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt
     4381 ggggtaacgt atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc
     4441 agattaaagg ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg
     4501 ttttagccaa ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc
     4561 tttgtttatt gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag
     4621 aagagttagc aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa
     4681 attggggagc agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt
     4741 cttggacgat gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga
     4801 aaggttgggg aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa
     4861 tcttgactaa tctattctta ttgactttct tagttactga ttatgcctat caatttgcct
     4921 attctttatg tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc
     4981 agttttcttt aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt
     5041 ttgaattgat gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct
     5101 atcttcctgg tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg
     5161 gaaaagaaaa agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc
     5221 ttgctgtagg ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt
     5281 gtagaagaat ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg
     5341 atcgaagcat tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt
     5401 ttcgaaaaac gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc
     5461 aaacaagaag tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat
     5521 ggagcgactg gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt
     5581 gtttcgatgc ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct
     5641 gttgactttg ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa
     5701 gccttagtca aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc
     5761 acattgctag atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca
     5821 tatgtattga tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc
     5881 gtagatgtct atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca
     5941 ggtattcgta ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat
     6001 cgagactaca cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg
     6061 aagaataaag aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt
     6121 ttaaaacaat gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct
     6181 acttctttca ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta
     6241 gaagaaacag gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga
     6301 ctaggttact gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc
     6361 ttgaaaaaac aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca
     6421 gtgtttttca tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact
     6481 cctgaagctt tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca
     6541 agcaagaagt tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat
     6601 gcactggaaa cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat
     6661 tggttataac tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct
     6721 tggacagcag ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat
     6781 tgccaaacat tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca
     6841 atcgcaagta ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct
     6901 atcaaagtgg caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca
     6961 tgtccatgca gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat
     7021 gcttggatga tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct
     7081 ttgaaaatct gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg
     7141 acgaaaaaca gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta
     7201 gggcgatagc caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct
     7261 gtaacagaac cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca
     7321 gttgcgacaa gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc
     7381 aatgcgacta attgatacac actagtcacg caacccatca aagaaatgac gattgccatt
     7441 cctaatgaag cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac
     7501 caaggagaga taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag
     7561 ttcaattgtg acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg
     7621 actaaactaa atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt
     7681 tcccaagttt ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct
     7741 tctttctgaa aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa
     7801 tgaaagtctc ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt
     7861 tatgagagga aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg
     7921 ctttcgagaa ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa
     7981 acgcttggat atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa
     8041 aacagcgcta acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt
     8101 gggaaaaagt ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt
     8161 ttcaaatcaa gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt
     8221 attagatgga atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt
     8281 aacgaaagaa gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct
     8341 ttccttacgt tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta
     8401 tttttggatg atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa
     8461 caattttaag atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg
     8521 aaaagtgact aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta
     8581 attaccatgt tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt
     8641 gaagaagaag aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg
     8701 aacgaaatcc atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt
     8761 tggaatgacg gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag
     8821 ggacttgcgt tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat
     8881 gaaacaacta attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg
     8941 tgctaatcaa aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct
     9001 tggtcattta ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca
     9061 aatggacacg ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg
     9121 acatcaatta aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat
     9181 tgcgacagat gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca
     9241 tttcaataaa ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac
     9301 tggattcaag caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc
     9361 ccgggcaaaa gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta
     9421 cgcggataat ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca
     9481 agaaaagaaa atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg
     9541 aaaagaacaa gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc
     9601 acaaaaactt gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa
     9661 attaaaaacg gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa
     9721 tcaacgctat ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac
     9781 ccataagatc gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag
     9841 tacgatgaaa gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt
     9901 gaaaaacgga gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca
     9961 ccatgcactt ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg
    10021 aaaaatctat caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt
    10081 cagccaaaca aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg
    10141 tgtcgtccaa gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga
    10201 aaaaggaatg agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa
    10261 accattgtca gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc
    10321 tacgcgtgca gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca
    10381 aggactgcaa gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt
    10441 aacaccagat ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa
    10501 acaagcagga accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat
    10561 caaatcaagc aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg
    10621 tggggccaat ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc
    10681 agattgcaac taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg
    10741 tcaccggaaa atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga
    10801 tggaacgaca gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga
    10861 agaacgacgc ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc
    10921 agcagcacta aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt
    10981 ggtcagcttc aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat
    11041 ttcggcttat ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt
    11101 gacaagactt ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat
    11161 attattggtg aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa
    11221 caggacgctg tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac
    11281 ataacaattg gattccacca atgaacagga agatcgagac catggaaggc catcctgatg
    11341 taggatcacc aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga
    11401 cacaagacaa agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga
    11461 taccatcaat cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac
    11521 gttcgcggtt ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt
    11581 ttgaaaatcg gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca
    11641 agcggaaatc acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt
    11701 agaatttttt cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg
    11761 tgccgacaca atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg
    11821 gcggatcttg cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc
    11881 cagcgtacag agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag
    11941 gataagctga ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa
    12001 taaacaaata ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag
    12061 gaacagtttt ttcttcatta aaacatggga caacaattga tactttcatc g
//
LOCUS       DACDSY010000087        12007 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDSY010000087 DACDSY010000000
VERSION     DACDSY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.088"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            584..916
                     /locus_tag="IWK44_001928"
     CDS             584..916
                     /locus_tag="IWK44_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9550430.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            1507..1836
                     /locus_tag="IWK44_001929"
     CDS             1507..1836
                     /locus_tag="IWK44_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550431.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            1823..2164
                     /locus_tag="IWK44_001930"
     CDS             1823..2164
                     /locus_tag="IWK44_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9550432.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDQGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            2278..2517
                     /locus_tag="IWK44_001931"
     CDS             2278..2517
                     /locus_tag="IWK44_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550433.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            2584..2823
                     /locus_tag="IWK44_001932"
     CDS             2584..2823
                     /locus_tag="IWK44_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550434.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            2909..3580
                     /locus_tag="IWK44_001933"
     CDS             2909..3580
                     /locus_tag="IWK44_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9550435.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            3633..5609
                     /locus_tag="IWK44_001934"
     CDS             3633..5609
                     /locus_tag="IWK44_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9550436.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGAAQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            5622..6278
                     /locus_tag="IWK44_001935"
     CDS             5622..6278
                     /locus_tag="IWK44_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9550437.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPAVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSIPLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKK
                     "
     gene            6374..7201
                     /locus_tag="IWK44_001936"
     CDS             6374..7201
                     /locus_tag="IWK44_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550438.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVTQQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETQTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIVL
                     LTPVSDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            7211..7459
                     /locus_tag="IWK44_001937"
     CDS             7211..7459
                     /locus_tag="IWK44_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550439.1"
                     /translation="MKIIKMLVFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINKLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            7490..9601
                     /locus_tag="IWK44_001938"
     CDS             7490..9601
                     /locus_tag="IWK44_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9550440.1"
                     /translation="MEGIMENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNVKLDFKYAGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMDRLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNKVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQD
                     PVEARLTYKEGSARVIDMSTGKDITSEGILTYDSHSRTLKWEASADFLNNNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQVIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(9639..11696)
                     /locus_tag="IWK44_001939"
     CDS             complement(9639..11696)
                     /locus_tag="IWK44_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP9550441.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
BASE COUNT         4165 a         1851 c         2409 g         3582 t
ORIGIN      
        1 ggtaattcac ttatttcccc aagctgaaaa gcctgatatt acttcttttc aggcagtgct
       61 gctaatattc aattacactt ttttgtatat ttttagttgt tttaaattta attgtctgct
      121 atgcttgtga tgaattgatt ttcgttctgc acttttggac gataaatttg gacaattgaa
      181 aatacaaaaa aaagaaaggc cctgtgagac tcggaaggct ggtaaccttc gtcgacgtcc
      241 acaggtggca ttaagatcac tcccttaatg cgtttacctt tcagtgttac ggtattaccc
      301 gtaacggatt tagtataaca gatattcaaa agtgatacca ctttttttgg ctgtgttaag
      361 gttaaaaata cgtgatatta cgatgaatct atgcctaagc ataatgttat aaaaatgtcc
      421 gtgtggctct ttgccgtatg acgaacgctg aaattagagg gaacgcatgt ttggatgcgt
      481 tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa aataacaaga
      541 aaaacttgtt aaaaatattt aaagtaagtg ggtggattat tttatgtcat atacaggaat
      601 tttgagtctc gaagatattt gtcactacgg taaacggtgt accgctacag aaaagatcac
      661 gaagaaacta tcaactggtc aaagtaaaac cgttgtgcag tgtaagaaat atataatcca
      721 gaaagacaaa gtctcagaag aaatgattta ttacattggg aaacaaaaac aaataatttt
      781 aaaagatcct atccctttaa aagagctata tccaacaatc aaacatgttt atgaccaaaa
      841 cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa aagggatggg
      901 gcgtttaatc agctgaattg atcattctca taacttgtat tttatcgttt ttcgcttgct
      961 tgaaaatatt gtcgagactt ctaaaggaac ttagaatcac tagaaaaata aggagtagac
     1021 ttgatctatc aacaattcct ttcggggggt gacggggtag gtttttacga ttggtaacgt
     1081 aaaaaattgg tcaatcgagt aaatgccctt gtttggatag tggtgaaatg aatttgtgta
     1141 aataatatgc acagagtaaa caagagggaa tttagaagtt agacaagtta gaatagcgcc
     1201 tagattaatc atgagcagct actaagggat ctaggctttg acgcaaactc atggaataag
     1261 gagtaggtct tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg ctgcagtaca
     1321 tgtagatact tgcgaagacg ttgatctttg gatcaatcga gcatttaccg ttttctacta
     1381 gggtttctcg tttcggacgc cataaacgag tcgatttatt ttatatattt ttgagtgaac
     1441 gaaaaacgat aaaaatattc gttatgaagt aaaactaatt tagtagatag caaaggtggt
     1501 agaggaatga ttcaaaaatt tgcgaaaatg agtaaaactg catggattga aaagcttgaa
     1561 aatgacttga gtgtcatgcg attagctcca aagaaattca aacgagagat tgcagaaaaa
     1621 gaagcaattt tgtcggtatt gaatgcggta gacgataaat gtccagtaaa aattagtttt
     1681 tcagatatag caacaattat cgaaggtatt tattatggtg aagttgatga agagatatct
     1741 tctacaaaag atttattaga ttttttggat aaaaacaaag atacctttat agtactaact
     1801 gattatgagg aggttacaac aaatgaaaaa ttatgatccg aatagccgat ggggaacaca
     1861 tacgattaag gtttctttcc aacgttggga ttacaaagga tttgtgacgt ttagaagagg
     1921 cggaaattgt aaaggattag atgttttagc attagacgag gatgatttat acgatcaaaa
     1981 attgactgat aatccaattg gattcggctt gctacctgaa gatgatcaag gtaatgaatg
     2041 gtttaaaatg acgcttatga atgataatgg agacgaatta tctgttgaag atacttggtc
     2101 atatttgagt gattatattg tcagcgttga aatcattgaa tttgtagcgg ataaagaaga
     2161 ataaaaactg gaggaataaa ttttacagat aaaggcttag aaggagtcac gacgattaat
     2221 aaaaaggtag tattacctta ggcattgagt aaggaattta tttaggagga aaattagatg
     2281 gcgcgaccag ttttagaggc gatggatatg gatactttga caaaaggaag gtatgtacag
     2341 ctgttagctc gtggttattc acctaaagaa gtattcaaat ccctgtctaa aggaacagat
     2401 atggaaaaag aaagattacg aaaagaattc gattattgga gaacccataa tggaatcaag
     2461 gatttgaaac cagcacggcc agtaattaaa cgaaagaaaa agataaccca agactgaagt
     2521 gtagagcgta tgtgatcata ggctttttta tttttgcaaa aaacaaggaa ggtaggaaaa
     2581 ctcatgaatt atgaagaaat cgaaaacaga aaaaaagtgt cgaaagaaat ggaagaaaag
     2641 ttgctaaaaa cgatgaaaca aaaacacctt aaaagattat ctgtcatgca atatatcaat
     2701 gatatgcaga taacaggaaa agagaaagct tgtttgttag gttcaatgaa aaactttgaa
     2761 cagttgagac gaacttatgt gaaaaaaagt tctaattgtc aactattgtt agaagtctca
     2821 tgacattcat taatagaatt catttttaaa actttttcta tggaaatgag aaaaagattt
     2881 tttgttttgt ctgaaagggg gtagctggat gagaaaatgg ttatttggtt ttttaggagt
     2941 cgctttaatc gtcttttgca gtgtgtttgg ctatgttagc taccaaaaac atgaaggaaa
     3001 ggtgtttaag caaaatatag aaaaaaagat gcccgtagat caaatcaatg aacatgcaaa
     3061 gagttataag gaggatgcaa cgaacgtcaa taatgatatg tctttggggc aaatgttgtc
     3121 catacaaaaa gaagcaatcg aaatgggtgt caataaacaa gcctttgcac aaattcagat
     3181 acctgcactt ggtttagcat tgccaatttt taaaggggca aatcaataca ctttgagtct
     3241 tggagcggct acttattttt atgaagatgc cgaaatgggc aaaggaaact atgtcctagc
     3301 aggtcataac atggaaatgc caggtgtttt attttctgat attcaaaagt tgtcattagg
     3361 ggaggtgatg gacttagtta gtaacgatgg cgtctatcgt tacaaggtaa cgcgtaaatt
     3421 tatcgtacct gagtacttta aattaatcga tggcgttcct gaagagaact ctttcttgtc
     3481 tttaccaaag aaaggagaga aacccttatt aacgttgttt acttgtgttt atacgtcaca
     3541 aggaaaagaa agatacgtgg tacagggaga gctacaataa caaggtttta atcaacgcga
     3601 gtcgttgaaa tatgaaatga ggagagtaaa aaatggcaat aaatcggtat gtaaaaaaag
     3661 ctggaatgct tgttggaatg ttaggaatca ttgcaagctg ctttcagggg tatcaagtac
     3721 acgcagaaga tgtcacgcaa aaaacaccac cagagaaggt caatatcacg gttcataaat
     3781 taatgtacga tcaaggaacg caattgaatg ttgatattga cgggatcaaa aacgatggtt
     3841 atacgcacga tcaatatcct acaggtgtaa cgaaatacaa caaggcagat tatggtgatg
     3901 ttgagtttac gttaggaaat attacggatc aagtattacc gagagaagat tctgatctaa
     3961 ccaacgcgaa agtggacgag attgtacaag atgtagaaga caagggatct aacagtgagt
     4021 acgtcaagaa tgcaaccaac aaaattactt cagctgtaga tgaaaatggt gagatcactt
     4081 ttactgatca accagcctat gtcaattcca aagggaatgt atatgtggtt tatgaaagta
     4141 aatccgctgc tggattagtc acacaaaaag caaaaccaat ggttgtgatt gcgccaatga
     4201 cagataaaac aggatcagga tttttaaaaa atattcattt gtatcctaag aacattgttt
     4261 ctaagttgag ttttgaatta acgaaatttg gtgacgatgg cgcagcccaa agcaaacaaa
     4321 cccttttaaa aggggcaaaa tttgagttat acaagggcga acctggaaaa ggaacaaaat
     4381 tgggtgatct tgtatcggat gatcaaggaa agctgactgc gactgatttg actttaggaa
     4441 aatactattt tgtggaagtt ccttcggaag ttgtagtggg atcagatcaa gaaccaacag
     4501 cagatcaata tttattaggc gcagacgctc gcaacgatgc gcataataaa ttaacctttg
     4561 aaattaccaa tgacggcgtg acaagtgact taaaagcttc ctacgtgaac tataaagcac
     4621 cagtgatcga taaaacagtg acaaatggaa caggacaaga acattctttc caaattggtg
     4681 acgcagtgaa ttatcaagga acgattcata tccctacaga tattgcaggt ggaacagatg
     4741 ggattacagt aaatggtgtg aagagtgaaa cttctcctta cagcgtattt aagtggggag
     4801 atacagcagg acaaggactt tcttatgtcg ctgctaaagc aaatatcaaa gtaacaaata
     4861 aagatggatc tgtggtatta aaagaaaata cagattataa aatccaaaat tcagaaaatg
     4921 gctttgtgat cgattttatc gtgaataatg gccaagtaag tgatactgta gcaagtttac
     4981 acggacaaga tcttcaaatg acttataaca tgtatgtaaa tgacagcgca gctgttgcca
     5041 atccattaac aaatagcgtg gatttcgttt ataacaacaa tccatttaat caggaagaac
     5101 accacgaaaa aacgaaagct gacgttgtga cttatggtgc aaaattctta aaagtcgata
     5161 gcggactatt tggaacagga atcaaagcaa caccgcttga aggcgcagaa tttgctgcta
     5221 aaaacgcaga aggaaaatat tatggtggat tagttgatac ggataaagac ggtgtcaaag
     5281 aagcagtatg ggtagacgat gtagcaaatg cagcgatcct aaaatcagat aaagaaggtc
     5341 actttgaaat cactggttta actgaaggtg aatattcact ggaagaaaca aaagcaccaa
     5401 ctggttacca aaaattaaca gaagatattg catttaaagt aaacaaaaac tcatttaaag
     5461 aggaaaatcg aatcactgtt aaaaataatc aaaaagcagc tattccatta acaggtagta
     5521 atggtttcca aacgtatgta ttgatctctt gcttgttgct aggagcaggg gcactttctg
     5581 cagttgttta ctttaagaag aaagcctaga aggatcagct aatgattatt cgtaagcccc
     5641 caaaaagaag agtcatgcac aaactgatga tatttttttg gttgctgtcg gcagtagcag
     5701 tctatttaat tccagctgtg gaaacaaatg tagcgaaatt tcaacaacag caaagaattt
     5761 tagccattga aaatcttgaa aaaacagatt cttctttgga tgttgcgaaa atagagttag
     5821 gcgatcctgt tggtgttctt acaattccaa gtattccttt aaaactacca atttatgacg
     5881 gaacttccga caaaatttta gagaatggtg tagggattac ggaaggaact ggcgatatta
     5941 caggaggaaa cggtaaaaat cctttaatcg cggggcacag tggactttat aaagataatc
     6001 ttttcgatga tttgccttca gtaaaaaaag ggcaaaaatt ctttattaag gtaaatgaag
     6061 aacaacatgc ctatcaaatt tatcgaattg aagaagttaa agcagaagaa ttacaaaaaa
     6121 attatttaga ttatctcatg cctgaaaaag accaagatag gatcacgtta atgacatgca
     6181 cgccaaaagg tgttaactca caccgctttt tagtgtatgg tgaacgagtg tcattcagta
     6241 aagaagattt aaaactacag aaaaaagcaa aaaaatagga aatcaatttc attggattgg
     6301 cgattagcac tgctattcgc tttttttgcg ttcctattat ttttatttat aaaaaaataa
     6361 ggaggagatc ggaatgaata agagaaaatg gctgtttgtt tgtgtagcag ctctctttgc
     6421 aactgcagct tactcacaga tatcttttgc tgatagcagt actactccaa ctaccagtca
     6481 gacaacagat agttcaacaa ctgcaacaag tgacacggac agttcgaaga acactgcaga
     6541 agaaacggca gattcaagta cgagttctac tgaatcagcg gaatcaacgt cagaaagttc
     6601 ttcagagact tcagaaaaac tgcaagaagt agaagatacg caagtaatca agcaaaacat
     6661 tcatattcaa aaaattattt cttcgaatcg aatcaacaat caaggaaggc aagtgactca
     6721 gcaagaaggt gtcaatggtg caaagtttat tgtctacgat atcacgaata tcttagaaga
     6781 aatgacacaa gatgataatg agaaaaatca atcggtagaa ataattgagt caaaattgaa
     6841 agatcgagca aaaaaacttt catacgatca gttgaaaaaa gtgactgaag gagaaaccca
     6901 aacaattgat gatcaggaag gcgtgattga tttttcaatt gaagtacctg caaacaaaaa
     6961 acaagcatac tatatcgtca atgaatcaag ccctgaaaac atttcaaata gcgaagatat
     7021 tgttttattg acacctgttt cagatgaaaa gggtgaattt ttgacggatg tttggttata
     7081 tcctaaatca aagaaatcag agccgaaaga agaaattaaa aaagttgtat caactggtgt
     7141 aaagaaaaac ttttttgata attgttggga ttttttcact catttatttt ttaacgatta
     7201 ggatgaaatg atgaaaataa taaaaatgtt agtttttaat ctatcttttt tagccgcaac
     7261 aatattgtgt tgcggtttag ttttttctca attatcaatt tacccaagat atcaacgatt
     7321 gttcgttcat tttctaaata tccagcatgc ttccatgcca ctagaagtga ttaacaagct
     7381 cttaatcaat ccaattttta tggctagttt gtttcttctt gcaggattgg taacgtgggt
     7441 attaggaaaa aaagaatgag aagcttacaa tcgtttaaca aaataataga tggagggcat
     7501 tatggaaaat aaaattcatt gcactacggg aagagtattt actttagtgt tgtttttgtt
     7561 ccttcttctt tttgcaaggg gaactgcagt ccaggcctta gaaaaagttg atccgagtca
     7621 aatcaaagaa aataataatc tggtacttta tcatgatagt gattcattga gaaatatcga
     7681 agtaactggg atcaatggat ttaagatact agatgagtat tcaactaaaa aacaaaaagt
     7741 acttgcttgg ccaaaagtca aagatttgaa aagcacgtca aaaattgttg cccattatca
     7801 aaaggtgggc acttatctag gaaaagaaat tgattgcgac gtaacttttt ctaattttgt
     7861 agcgcacgct ggggttaacc aagacggatt agctaaaaca attaatggga aaaagtatta
     7921 taattatttg ctctttgata aaaacattta tcgaggcttt ttcatgcagt caatcaacgt
     7981 tatgaatgtt aaactagatt ttaagtatgc aggatcaaaa acaaaagtag ctttgagtga
     8041 accacagcca gaaattaatt caaaaatgag atcgatgaga gcagctggta ttgagttgaa
     8101 tgatacattt ctatctattt acagtttaaa tggacagtat cagcaacgtg tgtcttggta
     8161 taatgacaat aatgaatctg tcggtgaacg taatattgat atgagagaat ttgttgggta
     8221 cgaaaaaatg gatcgcttac cttattttgt cacaacagat acagcatgtg cagaatacaa
     8281 agctcctgcg ttatcaacaa acaatttaac ttcaaaagta gtgggaggac gtgcagaaaa
     8341 ggcttatagc tcgaatgatc atttcaccga tgttgtagga gctgatactt atcacagaag
     8401 tggtgtaacg tatacgcttc aaggcgcttc cccaacattc atgattggcg caaatacgaa
     8461 tagtatgatg tttagctttg atactgcatt gctttggaca ccacaaccat cgaagcctac
     8521 aaaagaagtg tttaacaaag ctaatactga agaggcagca cacaatattg acaaaaaagt
     8581 gattccacaa ggatcagatg tttactatca tattcaccaa aagtttgatg cattaacagt
     8641 caacacaatg aacaaataca aatcatttaa aatcactgat acctttgaca gcaaaaattt
     8701 tgatatggta tcggatggga aaaactatga tggcgcattg tggtatgttg aagcgaatgg
     8761 gacttggaaa aaagctgata caggaggaaa attaactgta tcaggtaata cagtcactta
     8821 tactgcttct gacagctttt tagctaatgc tgcaaattac ggtagggact acattttgca
     8881 gctccatatg agagcaaaaa atgatgcaaa aggaattgca ccaaatgtgg ctactgtaac
     8941 gtttaacaat gatccgaaca aaggaacaca aaaaacaaat aaagtagaaa actatttctc
     9001 tcttaaacca aataaaaaag tggaacaaga tgggaaagat gttgatggtc gaaatgatgg
     9061 taaaaaagga acgcctacag ctcctttaaa tgcaggatct gaagtacaat atctagtcac
     9121 acaaaaatgg catacaaaag gtgtagacat tgcgctcgat cattacaagc aatttagtat
     9181 tcaagatcca gtggaagctc gtttgacgta taaagaaggt tctgcgcgag tgatagatat
     9241 gagtacagga aaagatatta cttcagaagg tatactaacc tatgatagcc attcaagaac
     9301 gctgaaatgg gaagcttccg ctgatttctt aaataacaat cttttagatg gacgagaaat
     9361 tcaactgata tttacagcta aaactccttt gaatgaagaa ttggatatcg acaaccaagc
     9421 aaaagtttca gtagaaagta ttgagaaagc aactaatgta gtaacgattg gggtaaaacc
     9481 taacttacct caggtcattg tgccaaaaac tggatcgaat catttagtgc taactgttat
     9541 tggaagtata ttatttctta ctttagcatc attgggctat gtagttttaa aactcaaata
     9601 aatataaaaa caaggctgat tttcagcctt gttttctttt atttgaagat tggattcgca
     9661 ctaataaata cattatctac gccaattcta ccagcagagt caactacaaa attatgacct
     9721 aatccgagaa tactttggtt attataaagt aaatttgcat tatgggcagg gctatttttc
     9781 catcctacca tggcatcgat agcaccatcg tctgataaaa ggtcatcaat atacagccca
     9841 gtttgactta tgttttctcc cttagcacca tagcagccta agctttccaa taaatcttgg
     9901 aaagtaccac caccagggcg atagtgactg aagctgactt gtaattcttt agctcgaata
     9961 tctgcagcct gttgaacaat atcattgaat tgcattgggg caacaccatt ttgttggcga
    10021 taatcattta accaagcaag ttctttttga cgcataaagt tacgtaattc gtctaatgaa
    10081 atctttttat atttgaatgt gattgtttta ttttgatcga cgttattaat ggatcgttgg
    10141 ccgtttttat ccacggtaac ggtaggatcg tcatttctgc acaagaaata attttgatca
    10201 gtcatatttt tccaaggagt agagaagcta tccccttcgt atactgtttt tgattcacta
    10261 gagagaacag cgttggtatc agcatttaca tgattaacag tcacattaaa tttacgagtg
    10321 tagttgaaaa tataattttg actttcagtc acattgttta aggttactga atttgttggt
    10381 tctacccgat cgtctaaaac atatccttga atagaaggag cagttaaagt gacactttca
    10441 ccgtgagtga cgttgattat ctgttctttc aaaatatcaa ctcgcccctc atttttgtag
    10501 ttgttattgc acaacaaatt gatttgatgc acttgattat aattgaatgt gattgttgta
    10561 ttacctgtga ttgttttgct ttgagtatca ttaccaatca ctttgtagct aaaattatct
    10621 ttagcacctt cgctatattt aaatgctttt gccttagcag tgtaagtaga accagaagtc
    10681 acttttgctt gatctgtagc aatcacttga cctgtttgat tgtcaatatg gttaatggta
    10741 accatatatt cttttggcgt ttcaggtttg tgttgcaacc ctttaaatgc agcatcaaga
    10801 ctatttttag cattgtttac ttgtgtttga gaaaccttag aatcgtctag tactttttta
    10861 gcattggata aagcagtctg aaaattattc caagattctt ctgtaaaatc tcctttagca
    10921 gttgatttta ctcggttgta taaagcttgt aattcagctt tattcacagt aggaactggt
    10981 tttttcttta atcctttgta agcagaatct aatttatttt tggcactatc cacttgcttt
    11041 tgagtcgctt ttgagtcatt taacactttc ttagcattgt ttaaagcggt ttggaatgcg
    11101 ttccaggtat tgtcggtata gtcacctttt ttcgttccct taacgctcgt ataaagagct
    11161 tctagagccg atttattcac tttgggttta gaggtgtcag cgccatacca accgatacct
    11221 tcgtatttcc agccatagtc tttggttaac cattgtactt ctttccaact tgcagtgtag
    11281 tgatgtgagc cagattttac accaggaagg aaaaggcggt agatcggttt attatttttt
    11341 tcggcagaac gccaggcagg cccttcataa gtccagccgt aatttttagt caaccatttc
    11401 acttcatccc aactagctgt gtaatggtga tcacgaagac ctttattgta caaacgatag
    11461 acaagatggc caccgctagt cggtgcttcc caggcggggc cttcataatt tccccatttt
    11521 atctttacta gatggtcacg ttcttttttg ctagaggtgt aaaaatgttc attattattt
    11581 ggattgtaaa ggcgatacat gacgttagtt gaagcagcgt caacagatcc agcaagacta
    11641 agtgaggcag caaatgttcc aatagctaaa acagtttttg gattaaataa tttcattttt
    11701 tcaactccta aatttaatta aaaatgttta catgattatt atatatcggt taaccgatat
    11761 agtcaataaa aaataagaaa agccttatat aattttggct tttcttattt tatttgttag
    11821 ttgaagtatt aatatcaacg atttcttcag ccaatttatt taatttcata gcatttatga
    11881 atctcatgtt gtctatacta gagcgtttgt tggcaaggtc acttactgta gttaagggta
    11941 cgccagattc tatggatata gaacgaactg atttaccact atcaaacagc aaccattcga
    12001 ttttctt
//
LOCUS       DACDSY010000088        11900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDSY010000088 DACDSY010000000
VERSION     DACDSY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.089"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWK44_001940"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWK44_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP9550442.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWK44_001941"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWK44_001941"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP9550443.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IWK44_001942"
     CDS             complement(2169..2906)
                     /locus_tag="IWK44_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9550444.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IWK44_001943"
     CDS             complement(2997..3524)
                     /locus_tag="IWK44_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP9550445.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IWK44_001944"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IWK44_001944"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP9550446.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IWK44_001945"
     CDS             complement(4401..4886)
                     /locus_tag="IWK44_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP9550447.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IWK44_001946"
     CDS             complement(5039..5236)
                     /locus_tag="IWK44_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550448.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWK44_001947"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWK44_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP9550449.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWK44_001948"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWK44_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP9550450.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IWK44_001949"
     CDS             9211..9423
                     /locus_tag="IWK44_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP9550451.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IWK44_001950"
     CDS             9425..11104
                     /locus_tag="IWK44_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9550452.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="IWK44_001951"
     CDS             complement(11358..11558)
                     /locus_tag="IWK44_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9550453.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11851)
                     /locus_tag="IWK44_001952"
                     /pseudo
     CDS             complement(11708..11851)
                     /locus_tag="IWK44_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
BASE COUNT         3447 a         2570 c         2067 g         3816 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa tttcatatta tcaagagtaa attacaaaag
    11881 aatcatttaa atcaatactt
//
LOCUS       DACDSY010000089        11607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDSY010000089 DACDSY010000000
VERSION     DACDSY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.090"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(172..1635)
                     /locus_tag="IWK44_001953"
     CDS             complement(172..1635)
                     /locus_tag="IWK44_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9550454.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(1699..4716)
                     /locus_tag="IWK44_001954"
     CDS             complement(1699..4716)
                     /locus_tag="IWK44_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9550455.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(4867..5706)
                     /locus_tag="IWK44_001955"
     CDS             complement(4867..5706)
                     /locus_tag="IWK44_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9550456.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            5851..6096
                     /locus_tag="IWK44_001956"
     CDS             5851..6096
                     /locus_tag="IWK44_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550457.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            6159..6581
                     /locus_tag="IWK44_001957"
     CDS             6159..6581
                     /locus_tag="IWK44_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550458.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(6675..6992)
                     /locus_tag="IWK44_001958"
     CDS             complement(6675..6992)
                     /locus_tag="IWK44_001958"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550459.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(7019..7225)
                     /locus_tag="IWK44_001959"
     CDS             complement(7019..7225)
                     /locus_tag="IWK44_001959"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550460.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(7206..7388)
                     /locus_tag="IWK44_001960"
     CDS             complement(7206..7388)
                     /locus_tag="IWK44_001960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550461.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(7402..7752)
                     /locus_tag="IWK44_001961"
     CDS             complement(7402..7752)
                     /locus_tag="IWK44_001961"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550462.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(7778..8080)
                     /locus_tag="IWK44_001962"
     CDS             complement(7778..8080)
                     /locus_tag="IWK44_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550463.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(8083..8316)
                     /locus_tag="IWK44_001963"
     CDS             complement(8083..8316)
                     /locus_tag="IWK44_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550464.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(9302..9624)
                     /locus_tag="IWK44_001964"
                     /pseudo
     CDS             complement(9302..9624)
                     /locus_tag="IWK44_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9747..10862)
                     /locus_tag="IWK44_001965"
     CDS             complement(9747..10862)
                     /locus_tag="IWK44_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550465.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(10868..11266)
                     /locus_tag="IWK44_001966"
     CDS             complement(10868..11266)
                     /locus_tag="IWK44_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550466.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         3083 a         2380 c         1775 g         4369 t
ORIGIN      
        1 atgaagaccc aaacgataaa aaatattcct aatactatat ttaataaact acgttctaga
       61 tagatacgag atttcaaatt aaaaaacgaa aatgagagat acaaatatat caaatattta
      121 aaaagtaacc acattttcta taccactcct tatcagaaac agccttattc tttagaataa
      181 gactgtttct gggttgatta atttgtcttt tgttaaacta ccatctaagg caagttctaa
      241 atgtaaatgt ggtcctgtac tatttcctgt tgaacccaca taaccaataa tttgtccttg
      301 ttcaactgtt tgtcctacgt tcacgcaata ttcctgttgg tgcgcataaa gagccacact
      361 tccgtcttca tgctcaattg tgacataatt tcctgacaaa gtatgatacg aagcttctat
      421 gaccgttcct gccttgctcg catagattgg ttctccttga ttggctgcca tgtctaatcc
      481 tctatgaaat tcttctcctc gccaaccgaa ggaactagaa attgaataat tttttagagg
      541 taccatatag ccttctttgc ttagttgtgg accagctact tctttatcat atttgattaa
      601 gtcataggtt tcgattaacc catttaattt tttatcgtag ctagtgtctg ttgcgtatct
      661 tcctgttaaa aatttagtcg cttcctgata ggtttttgcc ttacttttcc aaacaccatc
      721 ataaaaagag gtatttccaa ctaagccttc ttttaacagt ttggcatagt cttttaagct
      781 ttcttcatag ctctcatatt gccgaaaagt tgcttgggtc gtataaagtg tcccattgcc
      841 taaatcttct tgtgtcgcaa aagagacgcc ctttccgtta tgtgtgcctt tgattccaaa
      901 tagattgtag tttggtgcct gagccaattg actttgtccg cttgcggatt ctaaaatggc
      961 ttgcgcgatc ataacagaag cgtataaatc attttcttgt ccaattttac gagcagattc
     1021 accgatttta cggataaacg tttctacaga cgcgtctttt tcaaaatgaa tagtcccttc
     1081 gttatcttgt gtgctggaag ggactaattc ttgtatcggt gcgcttggtg cttggtggta
     1141 gttttttgtc tcttctgaac cgattgattg tgcctgcggt acgtcattca taggtacagt
     1201 cacactctgt tcaggtgcct tttgtgggcg ttccgttttt gttggcggtt ccgcttctgt
     1261 ttcggtactt ggtttttgag gagtctgtga gctttcatca gaaggttgct catttggttc
     1321 ttctgacggt tcctcctcag gttgttgatt gtcttctggt ttgtctggtt gattcggtgt
     1381 ttctggttgt tcttcagaac ctgttggtgg agcgttcatt tctggattat ctggtgtatc
     1441 tattgtatct tcagtcggtg tttctggttg attttcttct ccagtggaat ttgttgtcgt
     1501 ttcttcagtg atcgttgcag attgcgaatt tgtcagttga ttgttgcctt gttcattagc
     1561 caaaaccgct ggcgtatacc caaatacttg acctactaat aacaaagcag ataaacattt
     1621 actcgttttt ttcacaagat tgcctccatt cttcaatcaa tcatctagag acagctgatt
     1681 gctgtctcta gtcatacttt aattgctttg tttcatttta cgacgaacca gaacagcccc
     1741 cacaccaaca aaaagaaaca gtcccaatgc aattaacaca tcgttcactg tttctcctgt
     1801 ttttggtaag ctttgttttg gtgtgctcgt cggcgtttgt ttgacagggg ttgtcggcgt
     1861 ttctttttta atgccttttg ctgtcaatgt ttgtttttca ttcttcaaat caaagtgacg
     1921 tgcttcttcc tctttatggt ctttatcctt atagacaata tgagttgcga ccaatttctc
     1981 cccgtctttt aacgtgcctg ctttgtagtc aaaggatact ttaaactctt cagacgcttt
     2041 agaaacttta cgtgcagttt ctaccgtacc aacgactgta tcttttccat ttttatcaat
     2101 cttatggaat tgagtcaccc agtatttggt ttcccctacc gtagattctg gaaattcttg
     2161 cgatacatga tcgatcaact cttggtcttt gtctggatca aatgtttggt cgccattcac
     2221 tgtaataaag cgtgtttcga tttttggttc tttcgcagtc actgtttgat ctttattggt
     2281 taaatcaaaa tgacgagcga attcttcttc ctcttctttg tcgttataga caatgtgcgt
     2341 tgcgacaatc gtgtcaccgt ctgaaaccat gtctgctttg tagttgaacg tcacatcaaa
     2401 tgtttctttt tcatcggtca cttttcgtgt ttcttttacc gtatctaata cagtgacttt
     2461 tccatcggca gtaattttat ggaattgcgt cacccaatat ttctctgtcc caaccgcaga
     2521 tttagggaat acttgggaca ctttatccac taattcttgg tcttttttcg ggttgaaatc
     2581 ttttccacct gtcgttgtca caaacaaagt agaaatttct ggttggtctt cttctggaac
     2641 cacgatttct tcgtttttca cagtaattgg ttgtacttct ccgtctttga ccgttactaa
     2701 aatcgtttct gtttgtcctg acgcttgccc ataatgctca gggaacacaa tttccgtaaa
     2761 tttatacgta ccgtctggta attttagttt ttctggttct tcttttgttt catacacaaa
     2821 ggacacattt ttattatgtg cagtcatacc ttcaatcagg aatttcgctc ctggtaattg
     2881 tagatgattg attgaatcga ctttattgaa gtctacttca cccgttttta acaagttgtc
     2941 aatcacatct aattcaaacg taagcacagg cgtcgtgtta tcgccatagg ccacagagaa
     3001 cgtggtttgt tcttcagaag gttgacggta gttgtctggc acattttttt caatcaattg
     3061 gtatttgttt tgatcgccat tgtagaattt tttgtttgcc ttccagtgtc cgtctttgtc
     3121 agtttttact gaatccaaga cttctccttt tttcacaagg actgtttctt tatctggcgc
     3181 agtaatatct gttttggcga taatatcaaa ttcgatattt tctgcaggaa cggtatcata
     3241 aacaggtttg ccttgattcc aggattcttc cgcattatag acttctttga ctttctttcc
     3301 ttctggaatg aactgttgta agtcattttt tgcagttaac gtcacttctt tgtttggttc
     3361 aatcgtaatc gtatgagttt gtttgtctag aatataccca gctggtgctt tgacttcttt
     3421 gacatccaat ttattttgaa catattttcc gtctttttca tagaatgcag ggatttcttt
     3481 taaggcgatt tcccctttgg catccgttgt atattgtttt gtttctcccg tatcatgacg
     3541 tttgatttca tacgtcacat ttggaacttt tttccctgtg acagcgtcta gtttggttag
     3601 tttgaagtcg ccatttttta gaactttaat agttagtcca gctctattag gatctggcac
     3661 ataaagcttt cctagatttt gagcaacagg atctacatag gcaaaacttg aacctacccc
     3721 tctagaattt ggatccagtg aagaaacaga aattcgtccg tcatttgagt tttgatttgc
     3781 ttttataaac aaactatttc ctgattttct tacttgaacg ttagctgtgt tttcaacaac
     3841 cgacccatcc cattgagaaa acacaccttt tgtatcctta acttctactt cttcccctac
     3901 tttaaccgtc actgtttggc tatcaaaaga aggatcttca agtaaattgt ttatttttct
     3961 atctacagtc tgtttgaaag cttcataatt agattgtcca atagttccat aattaacaaa
     4021 tgaaccacct aaaacttccc aaatatatgt ttgtgctaat acatagttat ccattgaacc
     4081 actagaatag tagccaaaat aaccaattaa agaaatcttt tttctaatag cacttgtcgt
     4141 aattaaattt tcgagtggtg tcacatcata ttgaataccc gcactcgtga ttgcgccttc
     4201 ttgaatgcaa aaaacatctt ctccgtcaat ttgccagcga accatgtaat catgagtata
     4261 cattgttcct tcaggagtaa ttcgagtcat attcacatct ttacgaccat tttcagtcaa
     4321 tgttagattg gtagcacgtg tctgtgcatt cttttccaag attgcttgcc attcttcaga
     4381 ttcttgcgcc aatcgttctt gttcagaaaa agaagtcgct ttttgaatat cttcaatggt
     4441 tataaagtat ttctcataac cttcagggac ctcttgagtc attacattgg gcggagaaac
     4501 atcttctgtc gtgtcctgcg tagttgtctc ttctgttgtt tcacttgtgg tttcagtact
     4561 gatcgcttct tcactacttt ttgattcttc agtcttcatt acttcttcag tagaatttga
     4621 ttcatttatc gtttctgcaa atgccgttac agatcctaac atgggattga gaaatgtaac
     4681 gaaaattgcc aaccaagttg ccattctttt aaacatattt ttttcgttcc tttcatttta
     4741 tttttcagtc ttctaaagta atttcttatg gacttcgaca ccgaaacatt tttatcctcc
     4801 tcaaaacaaa aaaagactag aacttataat tctagtcttc atcaaccact tacttttctt
     4861 ttatttttag tagaaatcta cagtgtgttt ttgtgacccg tcactgtaaa taataacatc
     4921 caccataaaa cctttgttgt accactgcga atttggatcg ctaacctctg cattagccca
     4981 atcataggct tcatctacag tgttaaatac ctttccacta ttaccaatct ctccaactac
     5041 aattgttggc tcttcaggtg tcgttgtgcc accatttccg ccttgatcgc ctccaccagg
     5101 attgcttggt tcttcaggcg ttgttgttcc tccacctgtg cttgggttag aaggagttgt
     5161 ttctcctcca gtatttccac cattacctgg ttgtgtaggt gtttgagtag tgccaccatt
     5221 gttttctggt gtacttggcg ttgttgtacc accagttgtt cctgtttgat ctccagctgg
     5281 tgttgttggt gtcgttgtat ttggtgtcgt ttgtgtattt gaagtcgttc cttgtcctac
     5341 tggttgattc ggatcaactt tgtctttctc ttgaatcact gtttgagcca cagtttcacc
     5401 tgcgctgttt tgtacacttg ctacattgcc attaaatact aatttatttc ctgcatagat
     5461 taaatccaca tttgtaatct tattcatgtc cgctaatttt tggacagtaa tatttgttgc
     5521 ttgactgatt gcgcttaggg tatctcccca gacaattgtg tattcattcc ctttaatttc
     5581 tgctttgatt tgttccgccg aacgaggtgt ccaaactttg ttttctgctg cttcagcatt
     5641 cgttcccaca gcaagactaa ctcctccgac tacgattcct agtgccaata ctttcaacat
     5701 tttcattgtt tttcgcctcc gatttgaata aatttattta caaaaacata gtaccaaact
     5761 tatgtctatc agtcgatata ttttttgtag atttttttat tttttgatat actaactttg
     5821 gtactaaaat aactaaaagg aatgtcacac atgcaactta ataatttaaa tgaaatacat
     5881 gattgggctc aaaataattt acttaatcgt aaacaagctg ccaaaatcac tggacaaaac
     5941 tatgatgctt tttctagctc tttacgtaga aataatatca aaccatttct agaaataggc
     6001 gacgaacctg gtccttccat cattcgctta tatctaagag aagatattga aaaatacgca
     6061 aaacagctcg aagaaaaaag acagaaacgt caatgaggcg ttttctgtct tggatttaca
     6121 agtaaaacaa taataaacac ttttacaacg gaggttctat gaataagaaa tacattgttt
     6181 cagttggttc aatcgtactg attgtactcg ctattattgc tttttctgta tccaacaaaa
     6241 cagaaacaca gccacaaaat acggttcaat cgactactac aagtcaatct agcgatcatt
     6301 ccgtctatcc ttcactaaat agtttaaaag aaacgcatgg acaagattta attgatcttg
     6361 gttcttctca aattactact gtcttatttg aaaagaaaaa tattgcgttt attactccta
     6421 aagaaggaac tgaccttgaa ttttctatca aagtaacaaa taccaaagaa aatttattct
     6481 ccattcctga cattcaatat acccctaaca agttaaagat tggggatacg tttggtttgg
     6541 ctaaagtaaa tgaacaatac tttgcttact cactgaaata agtttctaat agcaagagga
     6601 aaaaccctct tgttattttt atcacataat cattttataa aaacgcttcg aaaatatcaa
     6661 catttttgaa gaaactattt ttctaatttc tcaatttctt ccaagggatt ttcgctaatg
     6721 acccatacag gcacagattg ctctcgaggc tccccttctt ctaaccacca acctgcataa
     6781 tgaacttctt cctcctcagc attaccatga taccaaccaa caaaggattc atctacaacg
     6841 aatgaaattt cttcttctgg atcaaataat tcatttttcc atgcttcaat tgcttgttct
     6901 ttggtgtact tttgtttgtt aaatacacca caaatttcgt catctacata aaaaatttca
     6961 aaatcaaaat tactcgttcc ttttactggc atcttaactt ttcctccttt gactcacttc
     7021 aatagatata ccactcatgt tcattccatt gtttactagt taattggagt tttatcttag
     7081 ggctttgtgc actctttcca aaaacttgag cgtcgttttt ttcttctaac caaaaaaggt
     7141 aatttcttac attcggtact aaatgttttt cctgcatttc ttcatgaaaa acatataatt
     7201 ctgcttcatt tttcaaccaa ttcattattc attccctcat tcacttcttc ataccgccat
     7261 tcagatagtg agatttcttt ttgtttctcc acttgtccta taaaaatcgc tgtattcaac
     7321 aagctgatca cacttaataa taaagcaatg aaactaatgg ttacacttac tctttcacat
     7381 tttgtcattt tgtttccttc tttactcgtt tgttatttct tgtaataggt ctaataactc
     7441 cttcaccgaa atttgcaaat ggttcgcaaa aatttcaata aaagcactcg cacacattac
     7501 cagaccttca actgatatat cactgcgaca ataaaattct ggagaaacac tgatttctga
     7561 ttctttctta caattgaacg aaaaaactat ttttgaatca aattccggta attcttccac
     7621 aactatgttt tgggattcta ccaagaaagg gagtgcctgt tctaatgctt cttctggtgt
     7681 ttgagcagat agaataattg cttcttgttc ttttatttca ttgttctcaa atactttata
     7741 actttttttc attgttagtt cctctttcat tcctctctca attagctaag ccgttactgg
     7801 tacgccattc tctaaattta gtatcgctaa tccttaatgc tcgtcgaata tcaatcattc
     7861 gataacctat tgcacgaagt tctttatatc ttttagcagt tagcgtctct aacgtcattt
     7921 caggcaatgg gttcgaatca aaaatagaat tcgaaccgat tatttgggcc aattgttgta
     7981 cttgtaacag ctcttctgaa cttaattcag tgattgctcg attgcctatc attgcataca
     8041 ttttctgtcg aatatacttc ttttcttgtt tagacaacat ttctatccac tcctattttc
     8101 attatcattt gttacctcca cttcagtaaa tccacaaata aatgaattca tattccaagt
     8161 accattccct tcaactacaa cttttctcct gtcctctttc ggatattcaa gaatcagacc
     8221 gtttaccaat atgtttttca ctactactaa tttgtttgta taccgtggaa ttgctgttcc
     8281 gatatacttc actttatttc ctacgctaat tgtcatttcc tcactcctat tcttttttat
     8341 ccaaaacttt ttcctgtcct attcctgcca acgaatatac ttcactgcat gcttaaacaa
     8401 aacaaaatgt tgccctgttt tatttccgtc ttttacttgt tccacataca gttcatattt
     8461 ggtcacacct tttaaaacgc cagatacttt ttttcatcca ttgtcacaat tagttttttc
     8521 atcaatcgat tgatactgca ctgctcattt ttcttgttca tatagtattc ctttcttttt
     8581 caaaactagc ctcttttgtt tactagtttt catcaatcat caacgattta acagattttt
     8641 tctttcgcag ggtttctagc gtcagcaacc ccaaactcta aaaaagtgcc tttgctccct
     8701 tgaaaaacga gcggacgatt gtactcgtca tcatatgttt ttcatactac ctgatccacg
     8761 caaataacct ttttttgttc tgtgccacac ccatccatac gtatcgcttg agattcacca
     8821 tactttttta ggtggtttat ccttcctaag tccctgttag acacgctgat taacttagaa
     8881 agtcctgttt gtatccaatt ttcaaagatc tcccaaaaga aataagtagg tttacgtgta
     8941 tcgcttctgt tcaaagtacc tgtttgtact tgagtatttc tactcccctc gttcaaggga
     9001 ttccttccac cagctagctt ttcctttcat catcaaatgt tcctgaaaag catttacgtt
     9061 tcgtgtccgt gtgcttactt attctttttt cgatagcgat tatccgctcg ctactcgcta
     9121 ttttatactt tgtttgttag ctcaaataaa aactaacaaa caaaaaaggc tagtttcttt
     9181 ccgttttatt tttttaactg tcgataaata gttgttagtt taattccaag ttctaaaacc
     9241 aatacgccca acaaaaccac aggaaaaaca attggggcaa acaagtaaaa tcggatagtt
     9301 ttcatgtctt ccctctttct ttttggtgtc gctgtggtcg ttcttgccgt tcaatacttc
     9361 gatacatttt ttattcatta tttgctcgtt ttgttttcct ctggactgct tttcttgctt
     9421 tttgaatact ttatacgctt ttcgtgcctg aattaatagg tcttgtcggc gtttcattcg
     9481 tgagaaaaca atatttgaaa ccatcaaatc ctggttcgaa caaccacgat tcgcaataat
     9541 tttcccatca aaaaaaatag ttttcacttg agaaaaaaac agttggaaat cttgttttgt
     9601 tgctaatttt tttgtcaaaa tcactgtgct tttccctgtt gaatctacaa tgattcttcg
     9661 aacaatccga ttcgataact tccaacgttg ccgattaaac aatgttactt cacaacaaat
     9721 atgacgtaag tgattagggg tcattttcat gtgtccaatt cctccaacca attatgcatc
     9781 gaaattttgg gcaattgtga cagcccataa atctgcgttt ccaggtcttc ataactacaa
     9841 gaacgcttgg cttgaagtaa tgaatattct gcttgatctg atagttttgt tgccattgga
     9901 tctagattca attctcttac acaacattcc caaaaattca atagcgctct aaaataaaat
     9961 ccttttcgat tctgaattgc ttttccttca tgacgagcaa ttttttcatt ttgaatacac
    10021 cgttgatacg ttttgcttaa atcttctgca aatgtttccc ctaaaattcg atactcgctt
    10081 ccatcagata aaccatgtgt ttgattatac tttcgttgcc gcttttctat aattcgttta
    10141 ctgtcgtata tcatgtttag ctgctctttc gcttcacgcc attcagctga actgcgaaca
    10201 atccatgtca aaacatcttt cggaacaaac gtactttcgc tgtaatgagc aataaactct
    10261 ttttgccaat catcagccat ttcaacagga ctagtcgtat cgatttttgt atctagattt
    10321 tctgtatcta gattgttaga agaattatta aataattgtg cagaattttc acatggattt
    10381 ttttcgtcaa aatcatcgaa aatcgctaaa ttatcaacag attcctcctc ttcttttcct
    10441 tgtgcagaat ctacactggt tgagtcaaca ggacctaatg aatacacatc attttctgtc
    10501 gtatctaatt ctcctagata caaccgagct ttgtttcttt ttcctgtttg ttgatcaaat
    10561 cccatatcct ctatttctag tagctcatat tctttcaatt cttttttgat tgtattaaca
    10621 gaacgtctgc aacaattcaa tgcattggct aattcttgtt ccgtaaattc aaaataaaca
    10681 aaaccttgac tatctacttg atttcgttga atggctaaat ctaagcgtga ccgaaaaacc
    10741 atatacgcga tttttgcttt atcagacaat gagcgatatt tttcacttgt aataaatacc
    10801 tttggaaatt gatagtataa ttcattgtat ttacgagttg cggtaaatct ttttgttgcc
    10861 atcgttatta cacctcatct tgtccatata ttaagaaaga attttggtgt tggtagacac
    10921 gaacatcttc cattgtagca atcaattctt ttagataaat tcgaccgaat tcaattgtta
    10981 gtaagtcgta atcttctaat tcatttttga tttcttcaat ttcttcttca gacacattga
    11041 gtaaaatggc taattcttga ttggtataag aaatatagac atgtttcttt tcatcaatcc
    11101 gtttttcaat taaggattct tctaagtact cattcagtaa gctataggcg tattttgctt
    11161 gtgcagtcat cgcataatat tttgacgatt ttataaagaa tagttttggc atatgaaaag
    11221 ttgcttgtgt cagcacatct gttgcgcgaa atttgttttt taccatattt tttcctccta
    11281 gaaaaacacg caaaaaaagc tctgtataaa tcgaattaca cagagtttct tcttgttgat
    11341 atttaatttc taaaatacgt ttcgggtaaa aactaaacac ttactaatat ttagattgta
    11401 gaatcttcgc cttcatttat tcattttaaa gagaaaaaga tcaagtgaaa tgtgcccaaa
    11461 agtttgattc tattaatttt taaacaccat tcataatata tttcctataa tgtaaaaagc
    11521 gtcgatactt attaaagcat cgacgttatt tttatagtaa atttatcaaa atttttgcca
    11581 acattttttc cgattttttt ccctatt
//
LOCUS       DACDSY010000090        11419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDSY010000090 DACDSY010000000
VERSION     DACDSY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.091"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            14..568
                     /locus_tag="IWK44_001967"
     CDS             14..568
                     /locus_tag="IWK44_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9550467.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(741..1214)
                     /locus_tag="IWK44_001968"
     CDS             complement(741..1214)
                     /locus_tag="IWK44_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9550468.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1226..1762
                     /locus_tag="IWK44_001969"
     CDS             1226..1762
                     /locus_tag="IWK44_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9550469.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1802..2173
                     /locus_tag="IWK44_001970"
     CDS             1802..2173
                     /locus_tag="IWK44_001970"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9550470.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2457..3728
                     /locus_tag="IWK44_001971"
     CDS             2457..3728
                     /locus_tag="IWK44_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP9550471.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            3973..4998
                     /locus_tag="IWK44_001972"
     CDS             3973..4998
                     /locus_tag="IWK44_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9550472.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            complement(5064..6404)
                     /locus_tag="IWK44_001973"
     CDS             complement(5064..6404)
                     /locus_tag="IWK44_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9550473.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(6563..7003)
                     /locus_tag="IWK44_001974"
     CDS             complement(6563..7003)
                     /locus_tag="IWK44_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9550474.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7163..7669
                     /locus_tag="IWK44_001975"
     CDS             7163..7669
                     /locus_tag="IWK44_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9550475.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7837..9750
                     /locus_tag="IWK44_001976"
     CDS             7837..9750
                     /locus_tag="IWK44_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9550476.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9755..10756
                     /locus_tag="IWK44_001977"
     CDS             9755..10756
                     /locus_tag="IWK44_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9550477.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10958..11164)
                     /locus_tag="IWK44_001978"
     CDS             complement(10958..11164)
                     /locus_tag="IWK44_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP9550478.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         3945 a         1789 c         2285 g         3400 t
ORIGIN      
        1 aagggaaaca atagtgagct ataatgtaca accgttaagg acaaatgaag agatacaaga
       61 ttttcagttt tggctaagaa gaacaagtaa tccggaaaga gatagttttt tatttttgtt
      121 tggaatcaat aatgggttac gtatgtcaga tattatagga ttaaaagttg gagatatacg
      181 aggcaaaagt aagcctatta ttgttgaaca taaaacaggt aagcgaaaac cgatttttat
      241 agataatttg agagaagaaa ttttattata tactgaaggt aaagaagaaa atgattggtt
      301 atttccgagt cggcaacaag gtcgacatat aacaagggat cgtgtctatc agatatatgc
      361 agatatagca gaaaaattag gacgagatga tataggaaca catacattaa gaaaaacttt
      421 tgggtatcac tactacaaga aaactagaga cattgctacc ttaatgttta tttttaatca
      481 tagctcgcaa gcaattacaa aaaggtacat tggtataact gaagatgaaa taggagcatc
      541 acttcgtgga tttaaactag gtgtataatt taaaaatttt tataaacagt aaagacaaag
      601 attaaaagct atttttatct agaacgattt tatttcataa agttcgcgga ataaaagctg
      661 gggttactgg tacttggtat aggttcgtgt tatataaact aagtaattaa ataacatttt
      721 taggttggaa aaggatgaaa ttaaaaaaag agaatcaata cggtctcaaa aacgcaaacg
      781 ctgcggaata agaaaaatac tgataactca acgtttagca gactatctaa tagggatagt
      841 ctgctttttt cgtttttgac catacttttg accttctgca aacttccttg cttttcctct
      901 gtagaactca tgaaatctgc aaaacgaatt gccgtttcct ctgtctgctt tttcgcgata
      961 tgggtgtata tatcatccat agtggtttta atatctgtat gtccaagtcg aacatacact
     1021 tctttgacag acgcaccaga ttcaaagagc agactacaat gggtatgacg aaaaccatga
     1081 agtgtgatat gttttaagtt attctcttta atgatgattt tcaaattgtg atttacatag
     1141 tctaaataaa ggggcttatt ttcttctgat gaaaacaaaa gttgttcacc atcttttata
     1201 ggtttgattc tcatttttag atattatgcc cgcacgtatt catcacattt ctattgtgaa
     1261 tcgctttatt cgaccgacat ttgactttta tcataatata ctcggtttaa aattactaat
     1321 gaaaacaatt aatcaagacg atcataccat gtatcacctg tttttttcag ataatcacca
     1381 ccgtgtggga actgaactga ctttttttga agtacaagaa gggaacaacc agttttttgg
     1441 aaccaatacg attgaacgaa cgatactaaa agttccttcc gaagcatcat tgcatttttg
     1501 ggaaatatat tttgaaacac aaggcgtttg ccattatgga atcgaatcat tttctggacg
     1561 acctatctta cgttttgaag ggccagatgc aacacgatta gctttagttc ccttacgcga
     1621 atttgaagat attgatgatt atttcccgta tgtcacagac cagatcccta cagagcatgc
     1681 cattttagga attgatgcca ttcaactacg cgtacaatat tccgatgcga ctgaacgaga
     1741 gttattgtcg gtaggctggt agcacaaaga atacgtcagt ttcttttcgg gtcatcaacg
     1801 agtgacaatc ttagaaaatc aagacactca gttctatcaa gaagttcaga ttattcaaga
     1861 tcgtgagaat ccagttgcca ttgaagggat tggtagcgtc catcatgttg cgtttggcgt
     1921 agaaaataaa tctgatttac aacgcatcga caaacaatta caagagcgca attttataaa
     1981 ttcaggaata aaagatcgtg aattttttgt atcactttat tatcgtgacc caaatcaatt
     2041 attgattgaa attgctacag gggaaggaaa cctagatgca aaagcgtatg aaaatcaaag
     2101 tccacgcttt gaagaaattc ctttattttt accgcagtac ttaaatttca ttcgtgaaca
     2161 agtggagcaa taattagcta atcaaatcca tgacgaagag taagaggttg actcataagt
     2221 caaaaaataa agaaaaacgc atcaaatcca ttctaaagaa tgttgatttg atgcggttcc
     2281 tatttttata aactatcatt ttcgactaat aaaactactt atgagtcagc ctctcttttt
     2341 ttcttgattt ttatacctct ttctaggtaa actaaacatc tgccaaaaat accgtgtctc
     2401 attgaaacat tctcaactat ttcaaaataa aggggagtac caaatgaacg agtatattga
     2461 aattatccac gcaagagaaa caacttaaaa atgtcagtct caaaattccc aaaaataaaa
     2521 ttagtatttt taccagcgta tctggttcag gaaaatcatc gattgttttt gatactatta
     2581 cccaagaagc tggtcgtcaa ctgaatgaca cctatgatag ttttacccgt ttatttttac
     2641 ctaaatacag tcgtccagag gttgatgaga ttgataatct ttctacggcg attgtggtca
     2701 atcaaaagaa gattggtggc agtgcgcgct caacgttagg gacgattact aacatcaatg
     2761 cgttatttcg tgtgttgttt tcatgttttg caacacctag tttaggatat gcaaatgctt
     2821 attcatttaa tgatcctagt ggaatgtgtt ctgtctgtca gggaatcggt aagacgattg
     2881 catttttaat aggattgctt tatttttcgg ctattgataa tgctgatttt gtattttctg
     2941 atccatttac cgcagaagtt ttgtctggaa ttgaggaaca ggcaaagaat catggttatt
     3001 ttgtattggt gcataatgtt aattcagcag aagatgttaa aactattcag agaaattggc
     3061 gttttgaggg gttcattgtt gttggtgtac gtgtaaaaga atttttggaa ttaaataacg
     3121 tttttgatgg tcctgttgtt tatattgata cgcatctttc aaaagagaca ttaagagaac
     3181 ttgaacaaca aatgaatcgt ttatttgtaa atacaaatga ttaccaagct agtacattgg
     3241 cagtggaaca tttgattcat tatggtcatg agaaaatagc gtttctttct tttgactaca
     3301 ttgtaggtga accaagtgtt attcaagaac gacatcaagc ttatatcgat acattaaaga
     3361 aatatcattt aacctttaaa tcgcattgga tatataacaa ctctgaattt gagcgaattt
     3421 atcaagaatt agctgaattt actgcaattg ttgttactgg agattattta gcggctaaat
     3481 tagtaaaatt tttaaaaaat aagaataaac gagtgatgga acaattatcg attattggat
     3541 ttgatgatat tacttttgca gaattaatgg atcctgcgtt gacaacaata cgactaaatc
     3601 caggtaacaa gggggcgatt gcttttcacc aactgattgg actggccaaa caagaagaaa
     3661 taaaaaatca agtactacta ttagagggag aattgatttc tcgagactct gttcacaaaa
     3721 tcaaataaaa aagctctgtg aaataagtat agttaacaat aaaatatccg aacgatagag
     3781 agaatgaact gtgatctagc agttcctcgt cgcaaattca ctagaattag cattatttac
     3841 tctaaattgt tcggatattt tttcttgttt ttaattatga ttacagtgcc tatttttaac
     3901 gaaatttgct tattttcaaa atgtatgatt atatgttagt aaacactttc aaatatgaaa
     3961 agaggagcta ttatggtagg aattagggat gttgcaaaac gggcgggtgt gtccattgca
     4021 acagtatcgc gcgtgctaaa tgaggatccg acactttcag taaccgatga aaccaaacga
     4081 aagattgcag aatcggttaa tttttatcat tatgtcaaaa aaagtcccgt tgcaaaaaat
     4141 ttaacaaata ttgcattaat aacgacagtt tctgaagtag atgagttgga agatccctat
     4201 tttcgaacga ttcgcagagg gattcaagtc gaagcagaac aaagcaaagt caatttgaag
     4261 aaaattattc gtttgtcaga aacaccgttg gagcaagacg atttgctaaa atgtcaaggt
     4321 gtgttgatta ttgggcaggt gctatcttct gtgattgaag aagtatcagc aattaattcg
     4381 aatgtcgtag tgattgatga ccctagtatt gaagctgaag tagatgcagt ttatactgat
     4441 ttaacaaaag caacgaatca gcatttagat cgattgtata aaaaaggaca ccgaaacatt
     4501 gcgtttatcg gaggaggacg tgttcgttta gatagccaag gaaataaaca cgtgtcagat
     4561 gatgattatc gaaaaatagc ctatgagacg tggatgaaac aaaaaggatt agaaaaatat
     4621 agtcaaattt atttagaagg ctggaccaca cttgacggaa tgcaggcagc agatttactt
     4681 ttgaaaaaac atgctaaaga taagttgccg actgctatca tggtaggaaa tgatcctatt
     4741 gctgtggggg tttatcgttc acttcaaaaa aatggtctac atattccaga agatatttcg
     4801 attgtgagtt ttgataatat tgaagttgct gaatttttaa cacctgcatt aaatacggtg
     4861 aatgtgaata cagaagaaat tggaaaacta gcagttcgca tggttaatga acgaataaaa
     4921 gaagttagaa atgtttcgat caaagtgatt gtttctaata atattattgt tagagaaagt
     4981 gaaaaaaata tcgattaaaa aaggctcttc gtcaatgagt gttggtgaag aaattcaata
     5041 gaataatcga gatactcaca cggctagaca gctatcttgt ctagccgtgt tgttgttctg
     5101 gctttagtca gttgggtagt gttcttagtt ttcttgaaat attttcctcg atataaaaag
     5161 attcgttctc ggtagttttg aaagtttcta aagccatagg caattcgttt tagtactttg
     5221 atatggttgt tcaaacattc tatagggccg ttggaatagg gaactcgcaa cgctcgtttg
     5281 atttctgttc gatatttctt gaaggtcgtg aacactggtt gatagtgctc tggcaactcc
     5341 ttgtagtctt cttttaggag cgcgacgaat tcaggaacat cttgtcttct gatagcggaa
     5401 agaaaaacct ggtagacttc gtagcctcgc tttaaggagt catcataata taatagccga
     5461 tcgactatct ccgcttctgt cagatgatct cgaaatgacg gacgccagta gcgatgctga
     5521 ccattcaatt cccacgcatt cttttgaagt agtctccagt atttctttaa gtgcttcgac
     5581 cgacgatccc cttttttaaa ggtcttcatt acttgaattc ggtggttggt aaaggcccga
     5641 ccgatgtgtt ggctaatatg aaagcgatcg gcgacgattc gggcattcgg aaaacactct
     5701 ttcaccaatg tgcgataggg tgtatagaaa tctgaaacca ctagacgtac ttgttcacgg
     5761 acttttctag ggtagcgcaa gaaatagtcg cgtaaaggat tgagttgccg attttccaca
     5821 atatcaatca gttgatgggt cttgccgtcc atcatgacaa agctcatggc tcccgagacg
     5881 tttttcaccg atttgaattc atcgaaacac accacttctg gtaacggctg gttcaaagaa
     5941 acggttttag gccgaaggct gcgaagtatt cgaatgacag tggtcggaga aatgtgcttc
     6001 atacgtgcaa tcaaagacat agaaataggc tcacttaata gctcaagaat ggcttgtttg
     6061 actctgcggg caatggagca gcgacgatcg ctaactgaat cttctgctaa aaaagtcttt
     6121 ccacagtgtt tacatttgaa gcgctgtttg ttaatccgca caatggtctt gtagttactg
     6181 acatcattca ataagatgga tacttttttc cagccccatt taatgatcgt ttgcttattt
     6241 tgatagtggc aatggaagca ttcctctggt tgataggtca atgtgccatc aaaaacaaaa
     6301 catgtttcgt cattaatgcg ctctttgcga aaggcatctt catgaaaggt aagatttagg
     6361 tctagaatat ctagtaactc tttggtaaac tctgaatagg acatcttgaa acactccttt
     6421 gaatggtttt cgtcgacttt attctaaagg atttcaaggt gtctgtttgc gtttattaga
     6481 aaaaagaaaa agtattggca acagaaggcg tcaccaacac tagatattat agatccagaa
     6541 aagcagattg tttatgccaa aattaagaca ttgcaaaaag attattttct ttaaaatcga
     6601 caagatgacc agaaagcaag agttcgttga gctcttgctt tcttttagtt ttagaaagga
     6661 acatcctgat tttatcttcg ctaaatccag tcaaaagttt tgcagattgg tcgtagataa
     6721 tcggtttttt caataaagaa ggggatgtac gaatctcaat atatagttca ctcaaactga
     6781 gttcatctat tctgtgaaca agatttttat aactaataga atgagtagag agcatttcgg
     6841 tcacgccgtt gtttgtcaat tttagtaagt ggataaattc atttttggtt agtggttgac
     6901 gaattaagtt ctgttcgata aacggtatat catggacgat aagccaacgt tttgcgcgtc
     6961 ttgaagacga gcagccagaa cctgtataca atttaatcat catacattcc tccaatagct
     7021 aagtatttat gaaataataa catgaagaaa tagtcgaaaa gtgccattta gaggtgtaaa
     7081 ataagaaaat gttgtcacgt ataatggaac tagctagata agaaatacta ttaaatcaag
     7141 aaaaaagaaa gaaggcacaa gtatgtcaaa cacagccgac gcaatagtag tgatcgattt
     7201 acaaaaaggg gtatgttttg attcttcgcc gatacacgaa ttagaaacag tgacagcatt
     7261 agtgaataaa agaatcaaaa catacgaaac gattgggaaa ccagttattt tcgttcaaca
     7321 cgaagatgct caattagtgc cacaaacaaa accatgggag ctattgtcgg aattatctat
     7381 tcctgagcac agttattttg tgagaaaaac ccatgcgaat tcattttata agactaattt
     7441 gagtgtttta ttaactgaat tggactgtca ttcgatcgaa ttatgtgggg cacaagcaga
     7501 gtattgtata gatacgactg tgaagtttgc tcatggatta ggataccagc ttatgatgtg
     7561 tagaggagca acgacaactt atgataatcc ttttatgaaa gcaaaggaaa caatcgcttt
     7621 ttacgagaaa atttgggata aacgatttct gacatttttg gagcaataag ttgtttcttc
     7681 gtttaaaaaa agcagaatgg atactggttt atccattctg ctttattacg taaaatgaga
     7741 aaataatcct gttctatttt tatgggataa accatttatt ttttgtataa aaaatgtttg
     7801 cgtttacata taattctcct agaaacataa aaaggagtga atagcttgag aacaaaacaa
     7861 gtattaaagg gagcgttggt catcttattt gctgtaagta caagcgtccc gcaatggaca
     7921 gtatatggag agagcatttc ttccgaatcg gaatctcggg aggtagaaac cacagaaact
     7981 actgatgctg gaaatgaaac gaaatcgagt acttcacaaa cagaaacaaa tactgaaaca
     8041 acggttgaga catctgttac tagcacagat caagaaacga ccgctaccac agataaccaa
     8101 caaacagaag aagcaacagc tacaaaaatg tcaaacgacg aatcctcttc tgctaaagta
     8161 agtgacaaag agaatgggac agaaaatgat gatatcggta aaggtgaaaa tttcaatggt
     8221 aaagaatatt tgaattactt cggtaaacag atgacagaaa tacttaatgg accaacagaa
     8281 gacatcaagt caaaattaat agagttatta gtcactgagc ttgctgttaa tgattatggt
     8341 gtagataaag agattatcaa acatctgatt gctacttatc tattgtacga tatcgctttt
     8401 gcaaaaaaca gtgtgttgat gtcgatgctt ggaggtacag atgatgggac aacgcctaca
     8461 gaaaacacag gcttctatcc aaatacaaaa caacttcatc aaactgtact aaacaaacaa
     8521 gatggcaaaa tgatggatgt atatgcatat tatgtcgatc aaggttcaga taaaacagtc
     8581 atgatccatg gcggatttcg agggaattgg aataacggga tcgttacaga agaatacaat
     8641 gatttttata aggccgggta taatttatta tttgttgatt ccagagcgac tggtaatagc
     8701 ggaggcgatt atgtgaccta tggacaatat gagtcagatg acgtccttta ctggataaat
     8761 caagaagttc gagagcggcc atctcaaaag attttacttt atggtggttc gatgggagca
     8821 gctacgatga tgtctgtatt agcgaaagat attcctgtca atgtcaaagg aattattgaa
     8881 aattgcggat ttgcatcaat tgatgaacaa ctacgtttta cgtacagcca gacagtagcc
     8941 cccgcactac cggatgccat aaaaaatcag ttagacatca ttggggatca agagcatgaa
     9001 gatttattta tgggcctttt gaaacaatac tattttgatc aggagatgca tttggataca
     9061 aaagctgctc tacctacgat tggaatgtca gattcattgc ctaaattgat tatccatgga
     9121 acagcagatg atgtagtacc tgtgtctaac gcgcaaaaat tatatgagtt atcaggaggg
     9181 tataaagatc ttttattagt agaaggagca gggcatggaa aggcacagga agttgaccat
     9241 gctgcctaca cgaaacatgt aacggacttt ttgaaagtag tgttccatga tcagataaac
     9301 gtaaagtatg tggatgaaaa caataaaagt ctactcaaag accaggacga aatccagctt
     9361 tacggagctt atggagaaaa ttatatgaca gagcaaaaga cattcgaagg atacgaatta
     9421 gcaaatgttg aaggaccgac tgaagggatt tttaatgaga cgataccaac tattattttc
     9481 tattacaaaa aaatcccagt agctccacca aaaaagcaag atccgacaga gaatgcagac
     9541 aataagggaa agaatgaacc tcgagaggat caatcaagtg atgatacgca accgattcag
     9601 atgacagtga aagtacaaaa aaagacagat gcaaaaaaaa gaatgttgcc gaaaactgga
     9661 gaaaagaaac attcgcttct catttattca ggtggatttt tatctgttag cacgattatc
     9721 acatggattt ggcgaagaaa gagagggtaa atgaatgaaa tggttcaagc gaatagtgat
     9781 tgcagtacca gttatcatcg tagttatttt agtcactgta tttttgatta atcaaatcac
     9841 accaaagccg attagcttgc ttgttagaaa acaatttgac acaagtgaaa aagaacaagt
     9901 atatgcccga ccagatgatt tccagcaaaa aatcgaacaa atcgatataa aaaaagaaat
     9961 atcctatcct tcaaagtaca aaaataataa tatggatatc tacacaccga aagtggaaaa
    10021 gaaaaaatta ccgattcttt tctggatgca tggaggcgca tatgtaggag gggataaaaa
    10081 tgattgtcgt gattatttgg aatatctatg ttcagacacg cagcaaatca tcgtaaatat
    10141 agattatgaa cgatcaccgg aagcaaaaca tcctacacca gttatccaat tgaacgaagc
    10201 cattcaagca gctaaaaaag agataaaaga tcaagcagat tggtcgaata tttcgattgg
    10261 tggaaattcg gcaggtgcac aaattagtgg agaatatttg ttagcacttc aaaatgaaga
    10321 aattaaaaaa gctgatcatc ttgatccgac tttggagaac aagcagatta cgaagtttat
    10381 ttctttgtct ggtctgttgg atccttctgc acttactcaa gtaagtgata aaatctctag
    10441 ctttctttat gaaaaatgtg gatgggctta ctttgataat aaggactttg aacaatcaga
    10501 gaaggtcaaa agtttagcct tggtcagaca tgcggatcgt tggacgcaaa acacattctt
    10561 aacggatgga aatacaaata cgtttacgaa acaaatggaa gaagtaactt ctgtatttga
    10621 gaaagcgggg gtgaaagtca caaaagtaga ttatgcggag aaaaatgctg ttttgaacca
    10681 tgaatatcag tttgatttca gcaataaaca agcgcaagaa acatatcaaa agttagtaat
    10741 gtttttcaag gagtaaagtc ttatgatgca aaagaggttg taatctgtca cttagctcca
    10801 agtcacaagg agcaatttca ataaacaagc ttttcatggt ttatgaatat tgtaacttgt
    10861 gactgaggag ttagagatgg caaggggcac cttttattcg aaatttggga atatcacacg
    10921 attattgtca cgatctcttt tacgtaaatt ttataagtca gatttgttgt tttgatcgtt
    10981 taaaaaaagc aacaaatccg aagcctgcgg aggcaagaag ggacaaaaag ccgagtggta
    11041 agcaaaagaa tgctggttcc actaatattc cgttgttatt gatataaggc tgggaacaaa
    11101 gatgaatagc aaataaaatc aatgcactag cgaaaaaaag caaagaaata tggatagctt
    11161 tcataagcag cacctccttt tttataattg tatgataaat agattaagaa tctataaatg
    11221 tgacaagttt gttaggatgt gaataaaatg atcatctagc gaacttgaaa caaagatggc
    11281 taatgataag caattaatga ttcagcaatc agataggtac aaagtttacc atcatacgtc
    11341 tagaccaata atagaaaata tgggaatata atagaaaaga tttaggatac ccccaaaaga
    11401 atttccccaa aaattcgat
//
LOCUS       DACDSY010000091        11416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDSY010000091 DACDSY010000000
VERSION     DACDSY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.092"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(229..1164)
                     /gene="murB"
                     /locus_tag="IWK44_001979"
     CDS             complement(229..1164)
                     /gene="murB"
                     /locus_tag="IWK44_001979"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP9550479.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1237..2157)
                     /locus_tag="IWK44_001980"
     CDS             complement(1237..2157)
                     /locus_tag="IWK44_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9550480.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2270..3022
                     /gene="xth"
                     /locus_tag="IWK44_001981"
     CDS             2270..3022
                     /gene="xth"
                     /locus_tag="IWK44_001981"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP9550481.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3886..4419
                     /locus_tag="IWK44_001982"
     CDS             3886..4419
                     /locus_tag="IWK44_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP9550482.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4909..5433)
                     /locus_tag="IWK44_001983"
     CDS             complement(4909..5433)
                     /locus_tag="IWK44_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9550483.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5426..5899)
                     /gene="tsaE"
                     /locus_tag="IWK44_001984"
     CDS             complement(5426..5899)
                     /gene="tsaE"
                     /locus_tag="IWK44_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP9550484.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(6022..7002)
                     /gene="pta"
                     /locus_tag="IWK44_001985"
     CDS             complement(6022..7002)
                     /gene="pta"
                     /locus_tag="IWK44_001985"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP9550485.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(7155..7835)
                     /locus_tag="IWK44_001986"
     CDS             complement(7155..7835)
                     /locus_tag="IWK44_001986"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9550486.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7961..8830
                     /locus_tag="IWK44_001987"
     CDS             7961..8830
                     /locus_tag="IWK44_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9550487.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8856..9371
                     /locus_tag="IWK44_001988"
     CDS             8856..9371
                     /locus_tag="IWK44_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550488.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9418..9981)
                     /locus_tag="IWK44_001989"
     CDS             complement(9418..9981)
                     /locus_tag="IWK44_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9550489.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(10036..10215)
                     /locus_tag="IWK44_001990"
     CDS             complement(10036..10215)
                     /locus_tag="IWK44_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550490.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10292..10801)
                     /locus_tag="IWK44_001991"
     CDS             complement(10292..10801)
                     /locus_tag="IWK44_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550491.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10897..11277)
                     /locus_tag="IWK44_001992"
     CDS             complement(10897..11277)
                     /locus_tag="IWK44_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9550492.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT         3277 a         2322 c         2024 g         3793 t
ORIGIN      
        1 tactgatttt atgatggatt atgcttgttt tttcatcagt tattctcatt ttttatcttt
       61 tttttgagaa tattgcaaaa aatggaaaaa tttatgagga aatattctat attttttgtt
      121 ttattgtgtt aaaatgtaca agatttgcgc catttgcgta aacaaccaca aaaaagagca
      181 ggacggccac cgtcctgctc ttttacttat tttaagttcc attacttatt aattggctaa
      241 acttctgctt gtttctgata ttgcttgttt ttctccaatg atccgcactt ctgtttctaa
      301 agacacatca aatttttctt tgattacttc ttggatgtgc gcaatcaatt caatataatc
      361 tgtcgctgtc gcatgatcga tattgacgat gaatccagca tgtttttcag agacttgtgc
      421 accgccccat ttcaggcctt gcaatcctgc ttgttggatc agctgacctg tatagtgtcc
      481 tactgggcgc ttaaagacac ttccgcatga agggtattct aatggctgtt ttgattgacg
      541 caattcggtc aactcatcca ttcttgcttt gattgcttca taatcccctg tttgcaaact
      601 gaatcgagct tccaatacga tagcacgtaa ttcttggact ttactgtgac gataagaaaa
      661 taccatttcg tcttttgtca aagttttcaa tgttccatct tcaagcaaaa gatccacttc
      721 agcaaaaaca tctttgatct ctccgccata cgcacctgca ttcatataaa ccgctccacc
      781 gacacttccc gggatgccgc aagcaaattc aaatccagtc aatgattctg ccaaagctgc
      841 ataagtcgta tcaatcagtg aggcacctgc ttccgcaatg accatcgttc ctttgacatg
      901 aatctgtttc atttcagtca gcatgatgac tacaccgcga atcccgccat cttgaacgat
      961 cagattgctt gcatttccta ataccatcca aggtatatca tggattcggc aataatcgat
     1021 gatttgcttt acttcttctt gttttttcgg gaatgcaagg acatcagctg gtccacctgt
     1081 tttcgtaaac gtatagttca tcaatggttc atcaaacaat atagtaatcg tggctaagct
     1141 ttcaactatt tcttctttat tcactgagga ttttcctttc ttttctcaaa cgtcctcatt
     1201 attctatcac gcttaataca atcttgccag tcttgttcaa agttgatgac ggtagtgatc
     1261 tttaagcact tcctcacata attcatgact tcttacaact tctttttgtt taagcaattc
     1321 tgtttccgat ttacctaaac ggatctgaga aaagaaggcc tctaccattg gctcaaaccc
     1381 gcgtttgact agcgtgtttg tccagtcgcc aaattctttc actttttttc catcagcctc
     1441 ttgaatgaca agctgggtca gatcgtttac ttcataagta ccatttttac ttgttacttg
     1501 atatttttct atattagcgc cacttgctaa atccatcgtt aaaatagccg tttgattttg
     1561 tgtttctaat tgtaaaatgg catattccaa aaattccttt gtttctctta tctggtattt
     1621 tgttctcaaa atgggttcat ctaataaata cacagctgta tcaataagat gtaaaaacaa
     1681 atcataaagg acgaactctg tcgtttcttt tgccgctatt cgattttttt gcaggtggat
     1741 caaccgctta tctgggatac gtttcaattc ttctaccata ggagcaaagc gccggttgaa
     1801 accgatcatc aaaattattt ggttcttatc agctaattct tgcaattcac gaacttcatt
     1861 tagatggaca ctcagtggtt tgtcaatata aacatggata ccattttcta gacatttttt
     1921 ggctaattca taatgtactt ttgttgctgc gtggatcata catgcttcga ttttttcttc
     1981 taaaagctca tcgatcgttt catatagatg ttggaacccg tactcttttt gaattctttc
     2041 ttttgtttct ttatttcttg ttgcaaaata aaaatcaagt gttccttgtt ttttcgcata
     2101 agttggtaaa tatgcttttt ctgcgatatt ccctacaccg attacaccaa tcttcatcta
     2161 ctttcctcct ggatcattta gttactttca tcttacacca ttttttacta aaatgaggta
     2221 caatagtatc gcaaaggtaa ggaaattaat tcgaaggagt gtgcttttat tgaaatttat
     2281 ctcttggaac gtaaacgggc ttcgtgccat cgtaaataaa aattttttag aagtttttca
     2341 tgagttagac gccgattttt tctgtctgca ggaaacgaaa ctccaagcag gacaaattga
     2401 tttagatctt ccaggttatt atcaatattg gaattacgcg gaacgaaaag gctattccgg
     2461 cacagcgatt tttgctaaaa aaccagcatt gaatgccact tacggaatgg gtatcgatat
     2521 ccatgataca gaaggccgtc tgattacttt ggaatattcc gatttctttc ttgtaacctg
     2581 ttacacgcca aattctcaaa gcgaattaaa acgtttagat taccgattag aatgggaaga
     2641 agccttttat aattatttgg aaaatctaaa aaaacaaaaa ccagtcatcg tgtgtgggga
     2701 tctaaatgtc gctcatcaaa agattgattt gaaaaactgg aaaacgaatc aaaaaaatgc
     2761 tggattcact ccagaagaaa gagcggctct ttctcgactg ttggataacg ggtttatcga
     2821 tacattccgt tatttttatc caacccagga aggtgtctac tcttggtgga actaccgatt
     2881 caattcaagg aaaaacaatg ctggttggcg gatcgattac tttttgacga gcaaagattt
     2941 agagccagga ctagctgatg caaagatcca tacggacatt atgggcagtg atcattgtcc
     3001 agtagaatta gatttgaaat aaagaggttg tgaaaaagtc gtttagtttc gagtattaag
     3061 aaaaattttg tgaaaatggc tttccacatt ttgcataaat ttggcataat accgagaaac
     3121 tggcttttga acactgttta tcgagagatt gtgaaaaagc tgtcctgcat caagtaataa
     3181 gaacagccaa acaaaaatag ttcctcatat ttttcgtttg gttgggctta ttaccgcaga
     3241 tacaagcttt tgaacaccgt ttattaagag gttgtgaaaa agctgcactg catcaagtaa
     3301 taagaacagc caaacaaaaa tagttcctca tatttttcgt ttggttgggc ttattaccgc
     3361 agatgcaagc ttttgaacac cgtttattaa gaggttgtga aaaagctgca ctgcatcaag
     3421 taataagaac agccaaacaa aaatagttcc tcatattttt cgtttggttg gacttattac
     3481 cgcagatgca agcttttgaa caccgtttat tgagaagttg tgaaatcagc gtcctgacct
     3541 gagtattaga gaaacaaatg gcaaaatagt tcctcatatt ttgctatttt ttgagctaat
     3601 accgagggtc agctgattga acaccgttta gtggagaagt tgtgaaaaag tcgcctactt
     3661 tcgagtcgta agaaattttt ttgaaaatgg ttcctcacaa ctttgcataa atttgactta
     3721 ataccgagaa gtcatgatcc ccatctaact ccaaatcaca aggaatgagt tcaaaaacta
     3781 gcttttctca acaaatatta cataaaaaat tctatgtttt ttgttaaatt tatttgcttt
     3841 tttcaaaagt atgttacagt aatttcccaa ggaggtttta cagaaatgaa ttttgttgcc
     3901 atggattttg aaactgccaa tcatcagcct tatagtgctt gttctcttgc gctagtcatg
     3961 gtaaaaaata gccagatcgt tgatgaattc tatacattga tacagcctga gactcctttt
     4021 ttctggcgaa atgtccaaat tcacggaatc catcaagaag acgtacgcaa tgcgccgaaa
     4081 ttcccagaag tctggcaaac tatccaacca tattttcaaa aaaatcgttt agtcgttgca
     4141 cataatgctg cttttgatac aaaggtctta gcgggatgct tagattatta tcatcttgaa
     4201 cagcctaatt atctttcttt atgtacagtg aaaagtagcc gtcgcctgtt tcctgaattt
     4261 ccaaatcatc gattgaacac tgtgtgcgaa aatttgaaca tccaattaga tcatcaccat
     4321 gatgcattgg aagatagtcg tgcatgtgcg gaaatcctgc tttatcaaga aaaaaaattc
     4381 gggacagatc cattgaaaaa acttgtgact ataaagtaaa agaatactta tgaaacacac
     4441 aaagaggtcg tgagcctgcc gtttcactcc gagtcacaag gagcaatttc aaaaaatagc
     4501 ttctcatgtt tattaaatat tgtgactggt gaccgaggag ctggcaggag aacaccgttt
     4561 attggagagg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagcc aaacaaaaat
     4621 agcttctcat atttttcatt tggttggact tattaccgca gatgcaagct tttggacacc
     4681 gtttaccaag aggttgtgaa atcagcgttt tgacctgagt attagagaaa caaacggcaa
     4741 aatagttcct catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac
     4801 cgtttattca aaaaaaatgg actgcgacac gacttttgtc acagtccctt cgtgtgtttt
     4861 tctgccttct caaaaggatg acagtttctc actcttttcc tatttcttct attcaatcaa
     4921 ataactcatt ttatatacat ctaaaaattc acctagatct gttcttgccc ctctaggcat
     4981 gaccgcttcg atttggaagc ccatttttcg gtaaaggtgt acggctcttt cattacgaac
     5041 ttgaacgtca agctccaatc gaaaaagtac acccatttcc ttcgcccaac tgatgatttc
     5101 ttctagcata agagtgccta gtcccattcc ccaatattcc tgcagaatac tgataccgac
     5161 ttctcccacg tgagacaaac gaaattgact gtctgccttg acagatgctg ttccaataat
     5221 cgtcccttca tagattgcaa gcagcaataa attattattg ctttcgtata tataatccaa
     5281 ctcttcttct aaggtttctg gtggtaacag gagttctgct tcatccaaaa ctaaaaattc
     5341 tgtctcctta ttaacttgtc ccatcatcaa aagaatgtct tttgcatctc ctggcactgc
     5401 ttcccgcaag atgatcccaa cttcttcact catgattttc agcccatttt tctagaattc
     5461 gctgtttgaa ggtttcactt tgctccccgt atgagattag tttcacatac cgcttttcta
     5521 aatctgtatc acttttttct aaaatcagtt ccaaataatc ctccggcagt gcttcttcta
     5581 atagatttcc ccattcaaca acggaaagcc catcaccttc aaaatattca tctaacccta
     5641 aatctgctcc gctagctgct acccgataaa tatccatatg atacaacgga atccgacctt
     5701 gactatattc tctaataatc gtgtatgtcg gacttttgat catttgcgtt atccctaatc
     5761 cttgggcaat tcctttcgtc aacgttgttt tacccgcacc tagatcacca gtcaatatca
     5821 gattgtcacc tggcattgcg gaatgaccaa ttgcctctgc gaattgttct gtcattgtta
     5881 atcctgatag ttcgatcatt tttcatcttc tcccgctctt ctttcttttt gccagtatat
     5941 cgcgtaatga agttttttta aagaaaaaat gaactagtca agaacagcag ctgctgtttt
     6001 tggctagttc gcgtgtattt gttaatcaat caatgtttgt gctgctgtga taatcgataa
     6061 tttatataca tcttcttcat ttgctccacg agacaaatca gaaaccggtt tgttcaagcc
     6121 ttgtagaatt ggtccgattg cttcaaagtt accgaaacgc tgagcaattt tatagccgat
     6181 atttcctgac tgcaattctg gaaatacaaa gactgttgct tgaccagcta cttctgaatc
     6241 tggcgctttc agttgtgcaa ctgaaggaac ataagaagca tcaaattgca attcgccgtc
     6301 gatcatgtat tgtggcgcta gttttttagc gattttagtt gcttcaacta ctttgtctac
     6361 ttctggcgat ttcgctgagc cttttgtcga gaagctcatc attgctactt ttggttcgat
     6421 atcgaacaat tctgctgttt ttgcagaatc tacggcgatt tcagccaatt cttgcgcagt
     6481 tggattcacg ttgatcgcac aatccgaaaa gacatatttt tcttgatctc ttccacgtac
     6541 catgatcatc gctccgcttg tccgactgat gccaggcttt gttttgatga tctgaagtgc
     6601 tgggcgaaca gtgtcgccag tcgaatggat cgctccagag accattccgt ctgcaatccc
     6661 catatacgta agcatcgtac caaagtaatt cacatctttt aaaatctttt ctgcttgttc
     6721 tttggatgcc tttccatttc tacgttcaac aaaagtatcc accatttcat cccacttatc
     6781 gtaattatca gggctgatga tcgtaaagct agatgttttg attcctctgg catgcgctgc
     6841 atttcttatt tcttcttcgt tccctactaa aatgggttcc acaagttctt ctgctttcaa
     6901 ccgggcagct gcgcctaaaa tacgagcatc agtagcttcc gggaaaacga ttttgatccc
     6961 acggcgaaca attttaaatc ttaaactatc aaacaattcc acaagatgac ctccagacgt
     7021 ttttatatgt attatcatac actattccag aaaaaaagaa cagtttattt tatctgtatc
     7081 agtcaaaaat gctggaaaaa ttaagttttt cacatacaaa gcgaactgca ctataacagg
     7141 aaagcgctgt tttattacac tgtttcaggc agttgccaat caatcggttc ttctcctaac
     7201 gcaaggagcg catcattcgt tttcgagaag ggtttggaac caaaaaagcc acgacttgca
     7261 gataacggac tgggatgtgg agatgttatg atgatatggc gactcttatc gatcattttg
     7321 attttttctt gtgccggttt tccccacaag ataaacacga ttggtttttc ccgttcattt
     7381 aatttttcaa tgatcgtgtc tgttaatctt tcccaacctt tcccgcgatg agaatacgct
     7441 tgcccttccc gaacggtaag aacggtgttg agtaaaagga ctccctgttt agcccatgac
     7501 actagatttc catgttgaac aggtgcgatt ccaagatcat cataaagctc tttatagata
     7561 tttcttaacg atggcgggat ctttacgcca ggttgcacag aaaaagataa gccatgagct
     7621 tgattttctc catgataagg atcttgtcct aaaatcacca ctttaacttc ttcataagga
     7681 gtcaattcca atgcttcata gatatgatac atatctggat ggatcttttg ggacgcatat
     7741 tcttttttta aaaattctct tagatgcaga taataatctt ttgaaaattc tgaggacaaa
     7801 acttcttgcc aactgttatg aatgatttct ttcaaaaact ccacctccta ataaaaagaa
     7861 taactcacat tttcttcaat cacaagggac aatcttcacc ctcagcactt gattaatatg
     7921 gtaaactagg aacaagaagc agaaaggaac gataatcatt atgatcaaat taattgcatc
     7981 agatatggac ggcacattat tagatgccca tatgagtgtt tctcctgaaa atgccgaggc
     8041 gatacgtttt gcaaatgatg ccggtgttga gttcatggtc gctactggtc gaaactatca
     8101 agaagctcgc gcagctttag atgaggtagg aatcgattgt gcgatgatca ctttgaacgg
     8161 ggcacaggta tttgataaag aaggaaattc attgtttacc gtgtcgattc caaataatca
     8221 agcaatctct gttttagata ttctagatgc taacggtatt tactatgaag tagcaaccaa
     8281 cgatggtctt tattctgaaa atcaagcgaa acgaattgaa agttttgctt ccatggtggc
     8341 tacccattta cctcacctta cgtataaaat ggcgattgcc atggcctcag ctaagctgga
     8401 gttattgcac atcacatacg tggacagtat tcgtgctatt ttggaagaaa agaatctgga
     8461 agttttaaaa attatttgtt ttcatacaga agggccagct gtattaggtc cagttggaaa
     8521 gcagatttct cttttaggcg atctagccgt tacttcctct ggtcaaaaca atatcgaagt
     8581 gaaccataaa aacgctcaaa aaggaattgc tgttgcccat gtcgctcacg agcgtgggat
     8641 ttctttagaa gaagtcatga cgattggcga taacttcaat gatgtcagca tgctgcaagt
     8701 agcaggagtc agtttcgcaa tgggaaatgc agaaatcgaa gtaaaagatt atgccaaata
     8761 tacaacggac acaaaccttg aatctggcgt aggaaaagcc atattgcgcg cgattgcaga
     8821 agacttgtag ttttatctag aaaagaggga tatagatgtc agaatttttc attcaggaaa
     8881 agcagcttgg acgtgcaact cggacaattg tcaaaaacga aaaaggacat tccctgtttt
     8941 tgatggttgg tcgctgggga acaaagggcg atgcattgtc tctttatgcc atgaatggcg
     9001 atctcgttgc tcacatcaag cagataagcc tgacattcgg tacgcgtttt gagctgtata
     9061 aagagttcga aaaggtggga accatgcaga aaatattcaa ttggcccggt gacttttatt
     9121 atattcgtag acttcactgg acagtccaag gtgatatcta taatcatcaa tatcaaatcc
     9181 atcatttcaa tcagcaaatc atgaaaatgg ataaagcaac acttttttca ggagattact
     9241 atgtattgga tgtctcaaat gatgaagatg ctccagtttg tatttgtatt gctgcggttt
     9301 tagattactg gctctaccgt aaagacaaaa gtcgcgataa gcctgtttct taccgactta
     9361 acttaagtta aaaagatgta cctgaaatgc ggaaatacgc atttcaggta catcttttta
     9421 gatattcttt tgcatatgat cataaagatg accactgatc atccgctgac cgacaacttt
     9481 gaagtcatgg cgttcgtata attttttagc ctttggattt ttttcatcca cacttagacc
     9541 gatgacggag cgattagctt tcttcgccaa tttaggtaat gcttctaata atcgcgaacc
     9601 gatgccttgt cctctaaaat cttctgatac actgatagag tccaaatacc attcgttggg
     9661 aaacgtttct ttatcaatga acaaacgtgt agacgcatct agctgatatt tctctaagat
     9721 aggtaccagt ggctgatcga tgattgattc ttcttcatct gtataaccgt aagcaactcc
     9781 tgcaacttta ccgtctattt cttctacgat acctcgtgaa taactgtaac gataatttgg
     9841 atcagtgatt gcttcttcta aaatctctaa agtcttttgt tcccctgtct gcttcaaaaa
     9901 tggcagttcc atatctttta gaatcactaa gatcaatggg gcgatttctg gagcatcttc
     9961 ttttctcgca aaacgaatca tagtttcctc tccttttctc cattctaaca tgcagaaaaa
    10021 gaaatcgcaa aatcgttatt ttaaaaaatc ttcaatcttt tcagtaatga aataaccatg
    10081 agtaacgatt cttgccgctt cagttcggca ataagggcag acgactggca tttctttatc
    10141 attatgttcg actacattat tgacttcttc gtatgcaaat ttttttccgc aattagggca
    10201 tttcattttt tccatgttat ctcaacttcc tttcatcttt actctcttag cttactctta
    10261 tattgtaaaa aagagaagca atatccttct tctatgcaga tatccatacc tgacggtcct
    10321 gttctccatc tattaatcgg actttataat aaggacgttc atctttgact agaagtgtcc
    10381 actctttcac ttttgctttt ttcccgtagt agctgactgc ttccttcatc gctgcttgcg
    10441 gttgtttgaa ctcttccgtt ttttctatat caaaaagatg ttctgcggcc ttttccgtgt
    10501 catttcgtac ggttgttttc ccagtggatg gattgactgc tatgacttgt ccttcacaaa
    10561 aaaactggta ttcatatgtt gcattcgtag gattctccaa atctttttca actgcaaaat
    10621 gaatagcaga tatgttatgt tcttttgttt cgttttttac gattgctgct gcttttttca
    10681 atgagatccg atattcctgg tcactaatat catccatgtg aaaagcggca ttgagcagtc
    10741 ccgtcatacc ggtaatccct attacgatga gggcagcaac accaaacatc atttttttca
    10801 ttcttcattc tcctgtttct tttttttaaa gaatatcaag aaatgaacga atgaaaaatc
    10861 gaatgaaacg aaaaaaaagt gacaattttg ttatctttat tcgtgcagtt ctaatggcaa
    10921 tccgtctgga tcaaagaaaa acgtcatttt ttttcctgta aaggtatctt ctctaatcgg
    10981 ttctgattcg atccctaagg actgcaattc tgcaattgtt tcttcaatat cgtctacttt
    11041 gaaagcaaga tgacgcaagc cacaagcttc tggatagttt ggtcgttgtg gcgcattagc
    11101 aattccaaaa atctccaatt cagcacttcc tagtttcaga tctaatttat agtcatctcg
    11161 ttctttgcgg taattttctc tgattacttc aaatcctaat ttattaacat aaaaatcacg
    11221 tgatttctga taatctgaaa caatgatggc tatatgatgg atcgttgtaa ggttcatatg
    11281 agcactccta ttcttcgttt aagctcggat ctatattaat ctccagttcc attgtaaatg
    11341 tgtcagattg aaaaaaatag ctgtttttcc tttttataag aaaaaaggca aaaaaaacag
    11401 aaagtaggtc ctccaa
//
LOCUS       DACDSY010000092        11299 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDSY010000092 DACDSY010000000
VERSION     DACDSY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.093"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(371..1639)
                     /locus_tag="IWK44_001993"
     CDS             complement(371..1639)
                     /locus_tag="IWK44_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP9550493.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(1870..2607)
                     /locus_tag="IWK44_001994"
     CDS             complement(1870..2607)
                     /locus_tag="IWK44_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9550494.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(2674..3243)
                     /locus_tag="IWK44_001995"
     CDS             complement(2674..3243)
                     /locus_tag="IWK44_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP9550495.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            3371..4243
                     /locus_tag="IWK44_001996"
     CDS             3371..4243
                     /locus_tag="IWK44_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9550496.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4585..5457
                     /locus_tag="IWK44_001997"
     CDS             4585..5457
                     /locus_tag="IWK44_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9550497.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            5454..7412
                     /locus_tag="IWK44_001998"
     CDS             5454..7412
                     /locus_tag="IWK44_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9550498.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(7744..8595)
                     /locus_tag="IWK44_001999"
     CDS             complement(7744..8595)
                     /locus_tag="IWK44_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9550499.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            8718..9185
                     /locus_tag="IWK44_002000"
     CDS             8718..9185
                     /locus_tag="IWK44_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP9550500.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(9448..9930)
                     /locus_tag="IWK44_002001"
     CDS             complement(9448..9930)
                     /locus_tag="IWK44_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9550501.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(10030..10893)
                     /locus_tag="IWK44_002002"
     CDS             complement(10030..10893)
                     /locus_tag="IWK44_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9550502.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         3322 a         2297 c         2070 g         3610 t
ORIGIN      
        1 gtatagagaa aagaaatttt ttgtagaatg aacggaaaaa atgataaaga aagcacaacg
       61 ctaaaaccaa gattattgat tcttatcaaa ctatagaaga tagctcttct gcagtagata
      121 attttacacc gccaaaaatt tccgaaatcg ccttttgcaa gaatcccgtc gaatattaga
      181 aattggatca gtatgcttaa acagtaataa aaaagttatg acaagatgtt gtcacaacct
      241 tttttgagta aacggtgttc aaaaacttgc atctgcggta ataagttcaa ccaaacaaaa
      301 aacatgaaaa tctgtttttg tttggctgtt cttactactt gatgcagggc agctttttca
      361 ctacctcttt ttaataacca taaaatccta ttccttcata tctccagccc atcggtgtta
      421 gccaatctct ttccttagca ctcaaggtat agtgatgggt accagttttt tcatttggat
      481 taaatagtcg ataaattggc ttggaaccac cagaataaaa acttagacct tcataacgcc
      541 aaccgatttt tgttaaagag ttgatctcag attgtgccat tgtgtaatga tggtctccac
      601 tgtaaggatt gtataaacgg tagacaggtt gtccgctgtt tggtgcttgc caagcaatcc
      661 cttcatattc ccagcctaca gttggcagat aatttttttc tccggcattc aatgtatata
      721 aatgttctcc tgtatgacgg ttatacagac gatacatcgg agaagttgaa gttggtgctg
      781 tatcatactg tgtcagatta taggtttcga ttacattgtt caatgtagaa ccataattag
      841 gcgcagtggc ataacgacct gttagccaag ccgtagcatc tttgtaagag ttcgtattgc
      901 ttttccatgc accggaatag tagtaaacgc ctggctgaaa agaagtcgtc ttcaataccg
      961 ttacgttatc ttgcaatgat tctttgtacg aaggatattt tctaaaagcg gcattcaccg
     1021 taatccattg cccattcaca tattccgaag taggcatcgt aacagattgg ccattatagc
     1081 ttcctttaat cccaaataga ttgtaattag gggcagaagc caaagtactg ttcccccagc
     1141 cactttcaac gatggcttga gcaatcatga ctgatgcata taagtcattg gcacttgcca
     1201 aactttgtgc agttgaggca atactatcaa tgaacgcttg ttgagaatta gcagatgagc
     1261 gagcataaaa agcatagcca gtatagtttt cgaattcaga caaatctgct tgatgggtat
     1321 gtgtcaatgt ttgcccatct gtcaattggt tcgtgatttc ttctgcattc tctggattgt
     1381 tctgagcttc tgctgatgta ctgtcttcgg tttgtgtttg ttctgttgtt tcagttgttg
     1441 gttcttcggt tgaaattgta ttttctgaga cagtagctga ctgcaaagta ctttctgtat
     1501 cgttttcaat tcctgtttct gaagaactcg atgtctgagt tgcgacagtt tctgtcgatt
     1561 cttcagctag cactgttgtg ttggcaactg ctagatttcc taaaagaata acgctaaata
     1621 ataaattctt tgctttcatc ttttttctcc tttgaattat gaaacttttc ctaaataaca
     1681 gtgtaactaa aaaagtaaaa gcaagtcaat gtccatattt aaataacatt tgtttctttt
     1741 taataaattt gctttttctg cataaaaaga cgtaaatgaa gaatattctt ctccattcac
     1801 gccttaaatt gatggtcgtt tgaataacta tttaacaagt aattctctct ggatattatc
     1861 cgttatggat tatacttcct gtgtcgtcgg acggataatc atgttgctga cagacatccg
     1921 gtctggcgtt tctatcgcaa atgctacagc agaagctata tcttcagatg ttaatcccca
     1981 ttctttttgc gcttcatgaa gttcaagagc agcctttttg tcgctgatgg tttgatataa
     2041 ttcagtttga acagctcctg gagagatgat cgtagatttg atgttatttt gccgttgctc
     2101 ttgacgtaat ccttccatga ttgcacggac agcaaatttt gttccgcaat aaacggcgga
     2161 atcaggataa acaacatgtc ccgccacaga atcagttgaa atgatatgcc cagatttttg
     2221 tttttccatg attggaagta cggcggaaat tccatttaac acacccatga tgttgatatc
     2281 aagcatattt tgccattcat cacggtgacc ttcaactaat ggagcggttg gcatgattcc
     2341 tgcattattg tacaatacat cgattcgacc gaacttttcc atcgctaaat cgataactgc
     2401 ttggacttgt gcaaaatctc ttacatctgc tgtttgaata tatagctcag cttctggtaa
     2461 agattctttt attgctttta ggcgatcttc tcggcgtgca gctataacta gtttagcccc
     2521 tttttctgct aatagcttcg ttgttgcttc accgattccg cttgaagcgc ccatgatgac
     2581 aatcactttt tctgataaat cattcattct attctcctcc tgttttgaaa aatctcttgc
     2641 ttatactcac tatctcttat ttcatcttac tgttcaaata atccgttcga caggtttgac
     2701 gattttggca gggattcctg caacgatggt attgtctggg acatctttcg taacagttgc
     2761 attcgctgca acaatggcat tctcgccaat cgttacgcct ggcagaatag ttgctcctgc
     2821 tccgatccaa gcatttttct tgatgtggat tggctttact gacaagcctc ttcgttcttt
     2881 tggatcggtt agatggttca ccgtgatgag gttgacacga ggaccaatca atactgaatc
     2941 ttctaaacag atgccaccta agtcaacgaa catcacttga cgattgataa agacattttt
     3001 ccctatacta atatgtttgc cgaaatctgt ataaaaaggc agcagaacag tcaccgtatc
     3061 atctactttg tcactaatga tattttctag ccgtttgcgg atatctgctg gttcatggta
     3121 cccagtattc agcgctgcta aaggctcttc gttttccgct tggatctgat ggatcacttc
     3181 aaagagcgga tccgttactc gaatctcttt attgacgatt tgtttttcga tgaggcttgt
     3241 cattttgctc cctcctttta tccttacttt ataacggacg gcttatgatg tctaatactt
     3301 ataaaacata cttaactatt ccttttaact atacttagtg gtatgctttt ttaaaaacag
     3361 gagggaaaaa atggatattc gtatactgac gtatttttta gcagtagcta gagaaaaaaa
     3421 tatcagccga gcagcaaaat ctctgcatat cacgcaacct actctctcaa aacaactgaa
     3481 agatttagaa gaagaacttg gtgtgacact tttcactcga ggctcccgag agatacagtt
     3541 gacggattct ggtcgatatc tttattcaaa aggaaaagaa atcttgtcgt tggtagaaaa
     3601 aacggcgaac aatttaacaa atgacgcaat catctctgga gaaatctaca ttggcggagg
     3661 cgaaactaaa gcaatctctt tcatttctga tagccttcat tcgttgatca aggcgcatcc
     3721 tgatatccag tttcatttat atagcgggaa tgcagatgat atcatagaca aactggatag
     3781 cggattactt gattttggac tcgtaatcga accgacaaac aaacaaaaat accagtcatt
     3841 tcgactgcca gcccaagatc gttggggact tttgatacat cggaatcatc ctttagcaaa
     3901 gcaaacagtc atccatccgc aggaactgaa aactgttcct ttgatcattt cccggcaaac
     3961 gtcagtggac agtcaactag ctgaatggct aggtttcagc atagatcagt tatcgatcgt
     4021 cggaacatat aacttattgt acaatgcttc tttgatggtc gataacggga cttttggtgc
     4081 attagctcta gatgggatca tcaatactta tgggacggaa aaagtattca tcccactcga
     4141 tccgccatta caagcttcac tgaatctcat ttggaaaaaa gaccagcctc tttctcctgc
     4201 tgctcgtaaa tttttagatg tcctaacgga gaaatacagt taagtagagt ttttttcctt
     4261 cgctttcagc tttttaacaa cgaataagtt aagcaaaaga tccaatgatt aaataacata
     4321 agtttcttaa tttgttcctt ccttttattt ctgataattc cagtaaatta ctataattat
     4381 aaaaaaagaa ttgacaaaac ataactgtct atataaaatt gattacgtta acaataattc
     4441 gtttcatgtg actagatcat actaggcatg aaaaatctct gaggaacatg gtttccctag
     4501 gaggtttttc gtgtctattt ttgtttggcc gcaatcaact ggtctagcaa acgtgaaaac
     4561 caaatcttca aaaagggtgc gcgcatgaaa atcaaaaaga tctttaatca aaatgcagta
     4621 ttggtagatg acggtggaca ggaaaaagta gctattggaa aagggattgg atttgacaga
     4681 aaaagaaatg atttgatttt tgatcgtgat gtcgaacgga tatttgtcat ggaaccagaa
     4741 ggtcaaatca agttgcaaaa tttacttaat cagattgatg aacgcttttt atttgcagca
     4801 gaaacgatca ttgatcatgc ggaaactgtt ttgatggaaa agttgaatga acatgtattg
     4861 atcgctttga ccgaccacat tgctttttct gcggagaaca ttaataacgg tatcgtcatc
     4921 cgcaacaaac ttttaagaga aatcgaagtg ctttacagtg atgaattcag tattgctcaa
     4981 tgggcagtag agtatctgac aaaagagctt gggattccat atacttatga cgaagcagga
     5041 tatatcgcaa tccatattca cagcggacga agcggtcgga acaataacca tcgcagtatc
     5101 cgtgaagtga cgattgtatc tgacatcatc catttagtgg aatcagaatt ggaaacagat
     5161 atccattcag aaccattttc actgaattat tcaagactgg tgaatcattt acgactgctt
     5221 ctacaacgaa cccatgcgca acaatatgct gttttagaca cggaaatcgt agaaatggtc
     5281 aaacgaaaat acccagaaag ctataagatt tctaaaaaaa tccgtgtgtt gctgaccaaa
     5341 gaataccagc tcgcgatcac gaaagaagaa ctgggttact tggccatcca tatcgagcgt
     5401 ttaagaagcg caatcgtaca aacaaatgtt aacaatcatg aggaggaaaa aaattgaaag
     5461 catatctaca acgaatggga cgttcccttc aacttccagt tgcggtctta cctgctgctg
     5521 ctttactagt agggatcgga aactggtggg catcttatag taacgacatt gtcgctcatt
     5581 ttttacaagc tggcggtaac gcagttttag gtcaattacc tttgctgttt gccgttgggt
     5641 tagcactagg gatgtcagtg gacaaatcag gtgctgctgc cttggcaggt ttggtcgcat
     5701 ttgaagttcc agtaaatgtt ttaaaaactg attcagtagc tactttattg aatatcaaac
     5761 cagaatcagt tgatccatct tttgctcaga tcagtaacgt atttattggt attattgccg
     5821 gattgattgc ggcagctctt tataatcgtt tccacgaagc aaaattaccg atggctttat
     5881 ctttctttag cggcaagcgt ctagtgccaa tcttggctgc ctttgtcatg ttgctgatca
     5941 gtgcagtgct actgctcgta tggcctccgg tttactctgc tttagtttct tttgctaaat
     6001 tcattttgag tttaggctgg ggtggtgcag gtctttatgg cttcttcaac cgtttactga
     6061 tccctacagg attgcatcaa gcaatgaact ccgtgttctg gtttgacgta gcaggaatca
     6121 atgatattgg taaatttttg gcttctgaag gaacaaaagg cgtaacagga atgtatcaag
     6181 ctggattttt cccaatcatg atgttcgggc ttccagcagg agcatatgct atttacagaa
     6241 atgcacgtcc agaaaacaaa gcaaaaacag cttccttgat gttagcagca gcttttgctt
     6301 cattctttac tggtgtaaca gaaccacttg aattctcatt tatgttcgtt gcttggccat
     6361 tatatgtctt gcatgcaatt ttcactggga tttctttagc agtgagtgca ttcttccatt
     6421 ggacagcagg atttgccttt agtgccggat ttgtcgatta cttcctatct ttgaaaaatc
     6481 cgattgcgaa tcagccattg atgttgatcg tccaaggatt agtaacagca gtgatttact
     6541 actttggctt taactttgct atcaaaaaat tccatctgat gactcctggc cgcgaggaag
     6601 cagatctttc agatgacgat actgcaacga ctaattcaga caataaatat gcggcacaag
     6661 caagcaaaat ctacgctgca ttaggcggag ccgacaatgt aacttcgatc gataactgta
     6721 ctacacgcct tcgtttgcaa gtcaaagata caggcctgat cgatcaaaat aaaatcaaag
     6781 ccactggcgt acctggtatg aagatcatcg acggaaaaaa tgcgcaagtc atcgtaggta
     6841 cagaagtaca attcgttgca gatgagatgg caaaactaca tggtggtgct gcaacacgac
     6901 ctgcacaaac aaacaccgtg gttaaaacag aaacatttgc cggtggaacg atcgaacaag
     6961 atatttacgc tatcgcaaat gggaaactga ttccaatcac tgaagtttca gatgatgtat
     7021 tcgcggaaaa aatgatgggt gacggctatg ctgtattacc agaaaatgga gaaatcttct
     7081 ctccaatcgc tggtacgatc acaaacattt tcccaacgaa acatgctgtc ggaatccaaa
     7141 cagatgctgg tatcgaagtc ttgcttcata tgggcatcaa cactgttgac ttaaaaggcg
     7201 aaccattcac tttatatgta aaagaaggtc aaaaagtcgc aagaggtcaa ttgattgctt
     7261 tagttgatct tgccgctatt caatctgcag gcaaaaacac ggatatggtc gttgtattca
     7321 caaatggcga caaagtccaa agcttagaaa tcgaacccgc acgtgatgta aaagcaaatg
     7381 ataaaatcgg ctctgtttcc aacaaagcct gagccatcgt cttccccaga cttttttatc
     7441 gataaacaaa caggagctga gacaaaaatc ttgtctcagc tcctttttcc gaataaaaac
     7501 ggcgttctcc tgccaactcc gcggtaataa gtccagccaa acgtaacata tgaagagcta
     7561 ttttttattt gactcttctt attacttgat gcagaacagc ttttttacaa cctctgataa
     7621 acggtgttca aaaaccagtt tatcggtatg aagtcaaatt tatgcaaaaa tgtggaaagc
     7681 cattttcaca aaatttttct tcatactcaa aactaatcgg ctttttcaca acctctttat
     7741 acattattcc tgatccaata tttcttttaa tgctgcttca agagaagtta atggttttcc
     7801 caatactttt tctaaatcat cagaggaaat agctaactga tcctttttaa tatcatgttg
     7861 gatcgctaaa aacatgtcag ctgcttcttg agggaatccg gcttctttta gattttcgac
     7921 aaacgctgca tcacttgctt caataatttc taacttttta cctgtcgcac gctctaatgc
     7981 cttggctaat tcttcatagt cagttagatt gccggataat tctaagattt ctggagagtc
     8041 actttctact agtacttttg ctgcagcttc tgcatattca cgtttcaatg cccaacctgt
     8101 ttttccattt cctgcagcat aaacgaattg accagttttt aaagcctgcc caatgatagg
     8161 taattcattc tctacatacc aattattccg taaaaatgta tgggaaatac ctgaatcctt
     8221 aatcattttt tcagtgaatt ggtgatctgg cgctaataca ctgatcgagt gatcggcatc
     8281 tgcaaaactt gtataagcga tataagatac cccagctgct tgtgctgcct tcactacatt
     8341 cttatgttcg gcttgacgat ttcctggaac gccagagaca aaaagcaatc gatctattcc
     8401 tttcaacgct tctttcattg agtctaaatc atcataatca ccaatacgta tattaaatcc
     8461 agcttctttc agtgcttctc ctttttcttc actacgtact agtacataga tttcattttg
     8521 tggaaccatc ttttttaaga attccagcgc ataaccacca aaacctccag ttgctcctgt
     8581 tactaaataa tgcatatttg ccactcccta ttctttttat ttgttaagat ttacagctgt
     8641 acatttataa attacacctt tatcataaag agtgtggtaa gataagtcaa gcaaaaaaaa
     8701 tgaaaggatg acaactgatg aagtattctc tgcaatttag tgatgcaatc catgtacttg
     8761 cctatattga aatgtttcaa gatacccgat cactttctag tgagatgatt gccagtagta
     8821 tcgaaacaaa tgcagcaaat gtacgtaaaa tcatgagtca gctcaaaaaa tccggtttga
     8881 tctttacaaa aaccggaaaa ccgcagcctt cccttgccaa aagtccagat cagatttctt
     8941 tactagatgt ctatcgcagc atcgaaggta atacacagct tatccaagtc gatccaaaaa
     9001 caaatccaaa ttgcgtcgtt ggtgcgaata tccaagaagc tttagaagaa agctacgctc
     9061 ggctgcagaa aaaggcagaa gaagaaatgg ctaagatcac acttgatagt ctgatacata
     9121 aaatagccgt tttggaaaaa gaaaaaagac cagaaaacac aggcctcgta gagagatttt
     9181 tataattctc tctactgtgc ctgcattttt ctgatcggaa tcgtctacat aaattctttt
     9241 attctccggg ggacaaaagc cttgtcatgc ccctttttcc tctctaatgc tcaggtcaaa
     9301 gcgctgaagt catgacgtct tgataaacgg tgttcaagta gctgaccctc ggcattagtt
     9361 ctgaaaaatg caaaatatgg aaagctattt cccatttttc ctctctaatg ctcaggtcaa
     9421 agcgctactt tcacgacctc tatattatca tggtttattg actaccggta tccagatttc
     9481 tcccatatat ttcccgttgg taactcccat ttcgactaca gcatttggac ccccaacata
     9541 ggcgtaatct tctacttctc ttaaaacatg cccaaaggcc gtattcgtca aatcatttct
     9601 taattcttca ggcgattctg ctaaagcgat tacaacaaga tacgttcctt ttgggaatgg
     9661 gatgattcgg gtagtgtttg gaacatcctg actcgtttga acaccgacat agtacatcat
     9721 tttcccgtta tagggttcgt tgactgtaaa gaaataatga ttttcagcag cttgtttcaa
     9781 tgcatctatt gttccattac cgataacttt ttgctcaaat gcttgtttct ctgcagccaa
     9841 accgacaaaa tccgtattgc tactcttcaa ttccacacca atacccgtca ccgtaaaatc
     9901 tggtttttct tcgatcctat actttgccat ttctcccacc ctttctatta tattaagttc
     9961 atcttacata aaattaaagg gaaatgacac gattacgctc ttttttctag ggatattttt
    10021 attttttaac tatttttttg ctttcactgc aatccaaatc tcactgtaaa cattactgaa
    10081 atcctctccc cattttgtaa aagagatctc gggagcttcg actaactcat aatctgaaga
    10141 aggaagccat tctgcataga ttttccccca agtatcttgt aaagtttggg gaaatggccc
    10201 tttattagga aaaatggccc aagtgtgttc ttctatagca agcatttcta aatcagcgta
    10261 cggattttct tttgtcgtag caaagccaat catgtgcatc aaactgccct tttcttccaa
    10321 gcgggctcca tcaaatccat aagaaacgtt gattacttga tttggatata aatctcctaa
    10381 cttatgcatc tcttcccttt gagtctccgt gatcgtctgc gctaactttt gtatttcttg
    10441 attgactcct tcaaatttga caggaacttt tttcgccaca cctgccacat aaaactgttc
    10501 tttcttttcg atacgaaatt ccatcgattt tcctcctctg acatcaataa aaaatgtcaa
    10561 gcgtggaaaa gatttttgta tgccgtatct gctgatttct gaaggcagat atcctgacca
    10621 atcacgaaaa gctcttgaaa aaccttcgac tgtttcatat ccatatctaa acgccgtttc
    10681 tgtcactctt gatccatcta ggagatcgcg attagccaaa gccaatctac ggtttttgat
    10741 atagtccatt agcgacatcc ctgcaataaa cgtaaagatg cgttttaatt gatattcaga
    10801 gattcctgta ttttcggcta ttattttata ggaaagtcct tctgtcaaat gttcttcgat
    10861 gtaatcgatc aattggttca gatctttaag catatttatc ttcctccttg cttgcctttt
    10921 tatcatatta tcaaagaaag tcaattactt gatgaatcag gaaccaattt agtcagatgt
    10981 agaaaatgtt gtatggatct aaagaaaatt ctcaagcagc gtttcgtaac ggataattgg
    11041 tattatctgc ctatctatgt tttaccttta gaaaaataaa ttttggggaa gacacattct
    11101 aagtaaacaa agcggtaaaa atatctttta ttcccttagt taatccgctt actacctact
    11161 gtacaaagca aattgttgtt caaactgctg atcagaacgc tgcttggatt tttctttgcg
    11221 atacatcata cctcttttat caattggaac aattatttat ttctaacaaa atcacttcat
    11281 ttttgtcaga taaataaaa
//
LOCUS       DACDSY010000093        11230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDSY010000093 DACDSY010000000
VERSION     DACDSY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.094"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(294..1142)
                     /locus_tag="IWK44_002003"
     CDS             complement(294..1142)
                     /locus_tag="IWK44_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9550503.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1306..2208
                     /gene="gnd"
                     /locus_tag="IWK44_002004"
     CDS             1306..2208
                     /gene="gnd"
                     /locus_tag="IWK44_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP9550504.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2210..3211
                     /locus_tag="IWK44_002005"
     CDS             2210..3211
                     /locus_tag="IWK44_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9550505.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3208..3492
                     /locus_tag="IWK44_002006"
     CDS             3208..3492
                     /locus_tag="IWK44_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9550506.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3525..4322
                     /locus_tag="IWK44_002007"
     CDS             3525..4322
                     /locus_tag="IWK44_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9550507.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4336..5169
                     /locus_tag="IWK44_002008"
     CDS             4336..5169
                     /locus_tag="IWK44_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9550508.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5187..6044
                     /locus_tag="IWK44_002009"
     CDS             5187..6044
                     /locus_tag="IWK44_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9550509.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6066..6575
                     /locus_tag="IWK44_002010"
     CDS             6066..6575
                     /locus_tag="IWK44_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9550510.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6612..7013
                     /locus_tag="IWK44_002011"
     CDS             6612..7013
                     /locus_tag="IWK44_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550511.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7125..7478
                     /locus_tag="IWK44_002012"
     CDS             7125..7478
                     /locus_tag="IWK44_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550512.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7512..7955
                     /locus_tag="IWK44_002013"
     CDS             7512..7955
                     /locus_tag="IWK44_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9550513.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7972..9057
                     /gene="uxuA"
                     /locus_tag="IWK44_002014"
     CDS             7972..9057
                     /gene="uxuA"
                     /locus_tag="IWK44_002014"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9550514.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9050..9763
                     /locus_tag="IWK44_002015"
     CDS             9050..9763
                     /locus_tag="IWK44_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9550515.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            10515..10715
                     /locus_tag="IWK44_002016"
     CDS             10515..10715
                     /locus_tag="IWK44_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9550516.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3780 a         1750 c         2411 g         3289 t
ORIGIN      
        1 aggaaatact taattcacca aaattttttt taatttttta gaaaataaga aacaaaaata
       61 ataaaaaaaa gaaaagataa attttttact actgtatact aactttgtta tcgcatctaa
      121 caagtaactc gaataatacc aatacaacct tctccaaaga tgcgatgaca tacgttctaa
      181 cccaccgact attgatgttc tgtaccaccc ttgcagaacc aaaaaagcca ctaccaaata
      241 tttggtagtg gctttttttg ttttatggat ttttcgcttt ttctgttatg gtgctattct
      301 ttgatttcag aaatagtatg tcgaacttta gcgatagaac gacgtgattc tttttcgttg
      361 taaaacatca aaatcacata tagagagtcc aaaatgctta gttgagaaat acgggaagct
      421 aaagcttcag atcggaattc tgtttcttca gaaatagaaa taaaaacgac atcgccaagt
      481 ttagcaaggg aagaattagc ttggctagta ataacgattg tttttgctcc agtttttctt
      541 acggtttcag caatatggat tgtttctttt gattgccctg tgtgagaaat caaaacagca
      601 cagtcttttt ccgtcaataa agaagcttcc atcaattgaa tatggtaatc cgtgctgtga
      661 gaagttgcga taggggaacg caaaaattta tgataagcgt cagttgctac aatctctgaa
      721 ccgccaacac cgaaaaaata gacacgttta gattcattga taatagtagc cgcttttttt
      781 agatcctttt gttgtaacaa cttttttgtg tcatttaaag ttgaaatact agactcgaac
      841 accttttgcg ccatagtgag ctcagtatct gttttctgaa tattttcgtg gatagaaata
      901 gcagatagat cattttcttg gatcaacatg gccattttaa aatctttaaa tccattataa
      961 ccaagttttt ttgtaaattg aaaaaaagta gagtcagcaa tgcccagttc acttgctaaa
     1021 ttgctaatag gactatgact tactttgctt ggattttcta aaatataatc ggcgatagcc
     1081 tgttcttttg ggctgaagtc gttgtacatt gttttgattt gtagtcttat ggagacgctc
     1141 atgagaattc ctcattttca taataagatt atcatttttt ccgttctcat taatgttaaa
     1201 gatacaagga gagtaggaaa aaatcaaaca aaaaatattt ttgtaaaatc tatttacaaa
     1261 aaaatttttt tgtataatga gggtataaga aatggaggcg ctatcatgga aattggtgta
     1321 attggtttgg gaaaaatggg attgaattta gctttaaact taaaagagca ccatcacaaa
     1381 gtacaaggac tcgacatttc aagtacagct gttagtcagg caagagaaaa tgacattcct
     1441 gcttatcaag agatctctga atttctagct acttttgagc agaaaaaaat catctggtta
     1501 atgcttcctg ctggagagat aaccgaaaac atgttaagaa aactttttcc aaaactcgga
     1561 ccaggagaca ttattattga aggcggaaac tcttattata aagattcaat ccgtcgagca
     1621 gaagagtttc agaaaaatga aatcggttac ttggattgtg ggacttctgg aggaatcgaa
     1681 ggagcacgac atcaagcctg cttaatgatt ggaggagata aagaaacttt tctatccgtg
     1741 gaacaattat tcaaagatgt agcaattgaa agaggatatc tgtatgctgg tgcatccgga
     1801 agcggacact tcttgaaaat gatccataat ggaatcgaat atggaatgat gcaagcaatc
     1861 ggtgaaggat tccaacttgt acaaaaaagt gagtatgctt ttgacttgga acaagtcgca
     1921 cgtgtttgga atcatggttc ggtgattcgc ggctggctga tggaaatcat ggaagatcag
     1981 ttaaacgaac atcccaattt aagtaattat cggggaatcg tttctgcatc aggtgaagca
     2041 aaatggacgg ttgaaacagc gctagaaatg gctgttccag ttccagtaat tgcaactagt
     2101 ttgttcatga ggaatctttc acaggaagaa gatagttttt cagcaaaagt agtttcagca
     2161 ctaagaaatg ggtttggcgg acatgaaata gttgaaaagg gtgagtaaaa tgccagatta
     2221 tcatatagca atcgttaatt ccagcagttt cggaagagtc tttccacacc acttggaaag
     2281 attaaagtca gttggagacg ttcatttctt tactgttgat caagagattc caggaaaaga
     2341 actagcggag ttacttaaag gattcaatat cattattgca agtgttacgc cattttttcg
     2401 acgagatttt tttgaaaaca aagatgaact gctattgatt tcccgacatg gtattggtta
     2461 caacaatatt gatttaaaag cagcaaaaga acaccagaca atcgtatcta tcatacctgc
     2521 tttagttgaa cgaaatgctg tagcagaaaa taatataact aatttgttgt ccatgcttcg
     2581 acgcacgaca caagcacaag caagagtcaa ggacgatcga tgggaagatc gggcggagtt
     2641 cgtcggtagg acgctgttta acaaaacagt tggtgtgatt ggtgcaggaa ataccggaag
     2701 cggagtaatc gaaattttgc gtaacggttt tcgctgtgat gttctagctt atgatcctta
     2761 caaatcagat ttatacatcg aaagctttgg tgcaaaaaaa gtagaacttc aagaattact
     2821 ggaacaatca gatatcattt gtttatgtgc gaatttgaca gaagagaact atcacatgat
     2881 ttctgataaa gaagtcgatc agatgaaaga aaatgtttat ctatctaata gtgcaagagg
     2941 cgctctagtt gatgaagaag cgatgattaa tggtctgaaa agcggaaaga tcgcaggata
     3001 tgccacagat gttttagaag aagaaccggg acgtcaagat catccgtatt tagcgtttga
     3061 acaagtagtg attacgccac atatatcggc ctacacaagg gaatgtttag aagcaatggg
     3121 tgaaaaatgt gtcagagatg tcgaacagat cagtcagcac cttcttcctg aacgttctgt
     3181 ccagacaaag agcaactata taaaaggatg atgaagaaaa tggtgaacgg tttaaaagtg
     3241 aaaacaggcc ctcaatttta tttatatgaa gaaggtggta tttcaaaagt ttctgatctc
     3301 ttgaaatcat acggcgcgaa aagagtactg gtcacccacg gaacggtatc atgggaaaag
     3361 gcgctaccta aattagtatt tttgaatgac gaaacgattc aattttttta tcatcgttat
     3421 tctggagaat gtagttatgc tgaagccaga cgaatagcta caatcatcaa aaaaatgaaa
     3481 tcgatttcgt agttggcgtt ggcggaggaa aactgaccga tctagtgggt tatagtgcca
     3541 atcttgcaag aatcgatttc ggagtcatcc ctactttagc tagtaattgt gctccatgga
     3601 cacctcttgc tgtcatgtat aaggagaatg gacaagcaga agggaaaaca gaacactttt
     3661 acagacaagc tgcttttttt ataacagatc cccaattagt gattgattcc cctgttccat
     3721 atttcattgc cgggttagca gatacactag caaaatggta tgaatctgag ctcatcttag
     3781 aacaagattt tttgcaagat gaatcatttt tgaagctagc acaagatact gcgaagatct
     3841 gcaaagaaga gattttgaat cattctgcta aagcaatcca agatatggca caaagaaaac
     3901 tgacatcgga gttcaaacag ctttcagaga ttatctttgc tgttgctggt ttagttggag
     3961 gattaggaga taaatacgca agaaatgtgg cagcacatgc tatgcatgac gctatcagta
     4021 aatatattcc ggaaagccat cgttttctac atggagaaaa agtagcgtat gggattttct
     4081 atcaactggc actagaaggt cgctgggaaa aaatagacga gttatatcct ttgtatcggg
     4141 aattagaact gccggtttct ctagaacaaa tgggattatt tccgaaagat aaacagataa
     4201 tagataaaat ggctgaattt attgattcga aagagaaagt gcatttgatt cctatagagg
     4261 taacacagaa atcactgata cagtcaatga tggaactgga aggttatatc agaaagatct
     4321 gaaggaggag gacacatgga aacattaagt gtagtacaaa gtttgttgat tgctttatgg
     4381 gtagccgcgg ttatgtcccg ctggttaggt ggaggagcca cgttgacctt acgtttttct
     4441 cctttgatga ctggattagt cgtcgggttg atcatggggg aagttgcaca agcgatgatt
     4501 gttacagccg ctttgcaaat gatttatatg ggggtttttt ctccaggagg atcaatgcca
     4561 gctgaaccat ctattgcagc agctatcgca gtaccagtcg cattattagg ggatctgtcg
     4621 ccagaagcag cgattgccgt tgcggtgcca gtcggattgc tcggaagtta cttgtatcag
     4681 tttcgttttt tcatcaatac ttttttaggt aaatatacag acaaagctgt agaagatttg
     4741 aatacaaaag ctatggcccg atcaattatt ctttatccaa cggttgcctc atttttatta
     4801 ttcgttccgc ttgttttcat tgctttatat ttaggggcgc cattgatcgc tgatgtaatc
     4861 aaagctttag agggaacagt cgttatccac attcttgaag tagtaggtgg agggttagcg
     4921 gcaatcggta tcgcgacaac tgtatacgtt atcggtcgaa aagattatct ggtctttttc
     4981 ttccttgctt attttatgag tgttgttttg aaatctttag atattacgat ggttacttat
     5041 gccatttttg ggattattat cgctttgatc tttgtccagt cgcaaaaagg gaaacaagta
     5101 cagcctgcca caagcaataa caattcagca tcttttgatg acgaagatga ctatgatgac
     5161 ggattttaat ttaggaggaa tgggaaatgg ctgaaataat ggataatcaa acaggtgctc
     5221 ctgaagaaat cacgaaaaaa gatgtaacaa aagcctatct gagatggcat tttgcaaacg
     5281 aaattcctca ctcttttgaa cgttacttgg cgccatcact actatacgga atgatgccaa
     5341 tcttgaaaaa actttataaa gatgaagact ctttaaaagc agcatataaa cggcagttac
     5401 tgttcttcaa cactcaatta agctggggtg gcggtgtgat tactggccta atgtcttcta
     5461 tggaacagca aagagcagaa gaagagtatg aagaaaaaga gatcttgatg caggatgact
     5521 tgatgtataa cacgaaagcg ggattgatgg gggcactagc aggaatcgga gatgcaatcg
     5581 attcaggaac cgtccaatat atttttatcg caatcgcggt accttgggca caacagggaa
     5641 gtcctttagg cgcacttttt ccttttatct gctttgggtt gtatcaggtt ttactagggg
     5701 tatttttcgc aagacaagct ttcactatgg gccgtaatgc tacaggccta aagcaaaaca
     5761 ctggtgttca gacagcgatt gaaatgctat cagttttagg gttgtttatg atgggggtgc
     5821 ttgcgggaaa ctatgtaaaa gtagaatctt cattgaaatt tgcgatatcc ggacgtgagt
     5881 ttgttatcca ggaaattctt gatcaaatcg tgccagggat attaccttta gctgtcgtaa
     5941 tgggtgtgta cttgttctat acgaaaaaag gattgaaagt cacacaagca ttattatggc
     6001 tgactggaat attaattgtg ctagcaggaa tcggtatttt ataaaaagaa gggaacgtga
     6061 ataagatggg tgtagtcaat ttagcgcgtg tggatgagcg tttgatccat gggcaagtaa
     6121 tggtaacgtt atctcaaaaa agtggagtga attccatttt tgttgtagac gaagttgtag
     6181 caaaagataa atttatgaga gatctttaca aaaatgcagg aagccgtaca ggacaaaaaa
     6241 caattgtcat tacaccagag aaagctaaat tctattggga tgaatatcaa tttaaagaat
     6301 acaattgtat tttgatcgca aaaacagtcc aaacgattta cgacttagta aaacacggaa
     6361 tccccatgga agaattgaat attggaggga ttgctcaaaa aaatcctgat acagatcttt
     6421 tagtgacgaa atctgtttat ttaaataaat cagatgctga aaaattgaaa gaaatgaatg
     6481 aacaatatgg cgtaaagaat atttattttc aagccactcc atctgcacct aaaacaggat
     6541 taaaagatgt attgaacaag tttgatttgt cataaacaaa tgcttagaaa aaagataaag
     6601 agggcgaaaa aatgaagaag aacgagctgt ttcgtgattg ggaatttcgt tatcgctata
     6661 tttaccgtaa gcgccgtaca aaaaagagta aacagcgttt tctttcagca ttagtatcag
     6721 atatttacag tatgagaaca gatgtgacag tcattgctta cgatacgcta gcctaccgat
     6781 caaaaaatat ttatgttgga gatatcgaaa aagcagagaa agttatctgt acttattacg
     6841 atacacccgt tcatgcatta ggaagttatt tcatgtttga ttggaaagac caaaggaaaa
     6901 aaacaatcta ctctatatta ttgtccttca ttttgttgtt cagtttaggt tggtggggaa
     6961 tgatgatata taacaaaaat cctcatcacg tattcgatct gctatccgta taaacaggta
     7021 tcactgtact agcatttggg agctactttt tcttattggg aaaagcagct cgcggatggc
     7081 ctagcagaca aacttttatt cgaaatactt catccatatt aacgatgcta gaaatgatcc
     7141 gtacgattga tgatccgagt gtagcgtatg cgtttgttga tgaaggctgt tatggagaaa
     7201 agggcttaga cagcgtgaga tcgggtatga aaaaagaggc tattcttttt tatctagact
     7261 ctgttggggc agatacaccg ctacagtttt cgggaaatta tttttcaaat aaagaacagt
     7321 ggctgaaaca agttgataag ttgaaagaaa aaaacgtcaa ttatattttt agtgcaagaa
     7381 aaaagcaagc gcagttcttc tatttgacca aaacagattt acgagggaaa acatttaatt
     7441 ggcaaaatgc caatcagatt attgctcttt ttcgataagt aaaaagtgca aaacaaggag
     7501 tgagaagact aatgttggaa atcatgatcg ttacacatgg taagttgagc gaaggactga
     7561 aagatgcggc ggaagtaatt gtcgggacat cgagccatat tgaaacaatg tgcttagagt
     7621 ctggggagga catccaaaca ttcggagaga aggtcaaaga aaaattaact atgatcaaca
     7681 cagcagaacc catgctagta tttgttgatc taattagcgc aagtccgtac aatcaagcgc
     7741 tcttggcagt caatgaactg actgaagaaa agaaagcagc cacttatgtc atagggggcg
     7801 tgaatctgcc aatgttgtta gaagcaatca atcatcagat gcttcacaca ccgatagaag
     7861 aagcggtgga acaaattgtc aaacaaggaa aagataattt aactgtttgg catgcttcag
     7921 cggaagaaaa aaaattagat gaggacgatt tttagaagga aggctgaaaa gatgaagtgg
     7981 ggatttagat ggtatggtga aaaaggggat tcgatcccat taaaatatgt tcgccaaata
     8041 ccgggaataa atgggataat tggaactttg ctgactaaaa tgcctggaga tgtttgggag
     8101 atttcagaaa ttgaggagtt gaaagcatct gttgagagcc aaggactgga actgcttgga
     8161 attgaaagtg tagcgatcca cgatgcaatc aaggcaggga cagatgaacg agattactat
     8221 attgataact atatacgaac aatcaaaaac ttgtctcgat gtggggttcg attgatttgt
     8281 tatagcttca aaccaatttt tggatgggcg aaaactgatt tgtttaatga aaacaacgat
     8341 ggaagctatt ctcttttata cgatcagaaa atcgtcgatc agatggagcc aggagaaatg
     8401 tatcaattga tacacagcca gtcaaaggga ttcaaattgc cgggttggga agaagaacgg
     8461 ttgaaaaaat tcgctcagct ggaaaaactg tatgaaggaa tgaccgaaga agaattattt
     8521 gaaaatctga cttatttttt agaaaaagtg attccagtgt gcgaaaaagc agatgtaaaa
     8581 atggctatcc atccagatga tccgccatgg gaactttttg gttttcctag agtgacgaaa
     8641 aatcttggcg acttgaagaa aattctaaat gcagtagatt ctccatataa tggactaacg
     8701 ctgtgcacag gatctttagg ggcaaatccg gaaaacgatt tagttgaaat catccatgag
     8761 gtaggttcgc gcatccattt tgtccacttc agaaacgtgg agtttttagg agaaagaaag
     8821 ttcaaagaaa cggcacatcc ctctacagaa ggctcccttg atatgtatca tattatgaaa
     8881 gcgttggtgc aagaagggtt cgacggtcct atacgccctg atcatggtag aaccgtgtgg
     8941 ggtgaagtgg ctatgcctgg atatggcctg tatgatcgtg caatgggact cacttacatg
     9001 caaggtctat atgaggcgat aagtaaggaa aaaccactgg gagagcatta tgaataaaat
     9061 caagctcttc atctttgata tggatggcct tttattagaa actggacgtt tagcttatcg
     9121 tgcttatgta aaatcagcac aaaagtatga ttatgaaatg cgtaaggaag tctattattt
     9181 actaactggc caaacagaaa tggcgataag agaacaaatg ggcattttgt acggagaaga
     9241 tgttccatat atcaaatggc gagaagcaat caatcaatat aaagaaaaaa tcgtgaaaga
     9301 agataagaga gtatatacga aaaaaggagc agaagaaatt ttatcttttg caaaagaaag
     9361 aggaatccat acaatcgtgg cttcgtctaa tacacgggta aaagtagaaa tgtatttaaa
     9421 aatggaaaat ctatatgcct tgtttgatca aatcatttca ggggatgatg taaaaaaagg
     9481 aaagccggaa ccagagatct ttttgaaagc ttgttcaaaa atgaatattc ctccaagtga
     9541 agcacttgta tttgaagact cgattgcagg gatcgaagca gcaaggcgag cgggcatttt
     9601 gtcttttctc atcaaagatc atttgatagg gttgcccgat cataatggga agcataagtt
     9661 gaaagtagat gtaagtttat atgaatccaa taaagcagac tatgaatttt atgacctgca
     9721 tcaagcaaaa cgatttttag aaagtaaagg gttgtatctc taataaagag gactattttc
     9781 tagtcctctt tatttattgt cctgcacgac tgagggaaca gatgaaaaag aagtaatttt
     9841 ttattttgaa gtatctacct tatttgtagt gaacaattta gaacaaacac aaaacgcatc
     9901 tactgttatc taccagtaga tgcgtttaaa gagaagaacg agaatcgtcc atatgagggt
     9961 ttccactcct tatgcagatt tttttaattc ttgcactgct tgttcgatcg taacacctgt
    10021 cgctacatga ttggtatttt ctttgtcaaa aacgacaaat agattcaaat ctgtatccaa
    10081 ggttactcga tattgtatat ctttgttata gaatgtcata gccattctcc tttctttaaa
    10141 tacttgctga aaggcaacga acgcgttgat aacgaaattc catttataag tataccacca
    10201 attgaaaggt tttcatagtt aagaaatttt aaagtcaaaa gttgcagata aaagcaaata
    10261 aacaagaaaa caaaaagtga atcaatcgcg gttattcaac tttttcttcg attcgaagac
    10321 aatagaagac aatgaatttt atatttcatt gcattatcct tgtgttatta gaaaaagcat
    10381 gatatattaa atgagtaatc tattttgaaa cgtaatttga gcgatatatc tacatagata
    10441 aaactccttt tgcctatcaa atatttattg caacgaaaaa acgtacattg atacgtatca
    10501 ggaggaattt atttatgaat aacggtacag taaaatggtt caacgcagac aaaggttttg
    10561 gctttatcac tggtgaagat ggaaatgatg tatttgcaca tttttcagct atccaaggtg
    10621 acggcttcaa aactttagaa gaaggccaag cagtgtctta cgatgttgaa gatggtcaac
    10681 gtggccctca agcaacaaac attgttaaag cttaataaaa attgactttt aaccgtcttt
    10741 tcgaaagaaa agacggtttt tttgtacgaa aaaattcatt ggtcgttgaa tgggctgaat
    10801 agtagtgtat aaaacaattt tgtggagcgt gaagctttta tggtagagga aaatacgaat
    10861 aaagtgatgt tgataaaaag attcctatct tgaatatgaa tcgagatgtc ttgaaaatga
    10921 atgagtttga ccaatcatta tcctatcgaa atttacagtt tcgttcgatt ggtctgtcaa
    10981 ttaatgaaaa agtaaaaaag aatatgttct aggaagaggg cggcacatct aaatttttaa
    11041 gtaacggaca gttcaaatat caagcattga aagaggctat cctactgttt gaaggaatac
    11101 gaatatataa aaacgacaaa gaggaaaaag tagttcatta aatcccttca agtgatgaaa
    11161 gaatgagatc taataaaacc tgatctttca tctgtttttt taataacgta ataatttttt
    11221 tattttttgt
//
LOCUS       DACDSY010000094        11058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDSY010000094 DACDSY010000000
VERSION     DACDSY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.095"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            20..1279
                     /gene="lysA"
                     /locus_tag="IWK44_002017"
     CDS             20..1279
                     /gene="lysA"
                     /locus_tag="IWK44_002017"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP9550517.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1266..2066
                     /gene="dapF"
                     /locus_tag="IWK44_002018"
     CDS             1266..2066
                     /gene="dapF"
                     /locus_tag="IWK44_002018"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP9550518.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2069..2950
                     /locus_tag="IWK44_002019"
     CDS             2069..2950
                     /locus_tag="IWK44_002019"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP9550519.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            2947..3663
                     /locus_tag="IWK44_002020"
     CDS             2947..3663
                     /locus_tag="IWK44_002020"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP9550520.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3669..4364
                     /gene="dapD"
                     /locus_tag="IWK44_002021"
     CDS             3669..4364
                     /gene="dapD"
                     /locus_tag="IWK44_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP9550521.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4380..5381
                     /locus_tag="IWK44_002022"
     CDS             4380..5381
                     /locus_tag="IWK44_002022"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP9550522.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5378..6739
                     /locus_tag="IWK44_002023"
     CDS             5378..6739
                     /locus_tag="IWK44_002023"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP9550523.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6699..7850
                     /locus_tag="IWK44_002024"
     CDS             6699..7850
                     /locus_tag="IWK44_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP9550524.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7825..8994
                     /locus_tag="IWK44_002025"
     CDS             7825..8994
                     /locus_tag="IWK44_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9550525.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9169..10548
                     /locus_tag="IWK44_002026"
     CDS             9169..10548
                     /locus_tag="IWK44_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9550526.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10829..11058
                     /locus_tag="IWK44_002027"
                     /pseudo
     CDS             10829..11058
                     /locus_tag="IWK44_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3667 a         1938 c         2490 g         2963 t
ORIGIN      
        1 tgaaaagagg aatgaaaaaa tgacagaatt gataattggc ggagtcacag caagcacatt
       61 ggcagaaaag tataagacac ctctgtatgt gtatgatgaa gaaaagatga gacaaacgat
      121 gctgtcattc aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc tttacgcctc
      181 taaagcattc caaacgattg agatgctgca tttagttcgt gaatatggat tcgggctaga
      241 tgttgtcagt ggtggggaaa tctataccgc tttgcaagct gattttccta gagatactat
      301 ctacttccat ggtaacaaca agacgccaga ggaactgatc tatgcacttg aaaatgattt
      361 gttgcatatt gttgccgata atttgatgga agttgaatta ttagcagaat taagccaacg
      421 ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag cacatactca
      481 tgaatatatc gtcactgcgc atattgattc aaaattcggc atgagctatg aaagtaaaga
      541 gtgccaacaa gcccttcagt tgataaaaga aagcagctat ctagagttag aaggatttca
      601 tgctcatatc gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa tcgacaaatt
      661 gacgaattac ttaaccgatt ttgaccaacc tctttccttg aatcttggcg gagggtttgg
      721 gattcgttat acacaaaatg atcagccgat cccagtcgaa gaggcactaa aaaaactgat
      781 tgaatatacg gaagaagctt tggaaaaacg aaaactgacc attcgtcaat tactgatcga
      841 accgggtaga agtattgttg gagaagctgg tacaacacta tacacgattg gtttcatcaa
      901 aaagacacca cataaaaaat attactttgt agatggcggg atgacagata atatccgtcc
      961 agctctgtat caggcagaat acgattgtga tttagcagca aaactgtcgg aagaaaaaac
     1021 agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa ttaaagaagc
     1081 ttatttgccg aaagcagaat cgggtgatct tttagctgtt tattccacgg gagcttatgg
     1141 atactcgatg agcagcaact acaatcgagc gacacgacca gcagttgttt ttgtcaataa
     1201 tggccaatca agactgattg ttcgcagaca agcattttct gatctactaa ggggggaagt
     1261 ttcctatgat ccttcataaa tataatggct gcggaaatga ttttatctta ttagattacg
     1321 cagaagacac agattatagt caattagccg cttctttatg cacaaaagaa aaatatgata
     1381 cagacggatt gatcgccgta aaaatcgatc cgttggaaat gatctattac aacaaagatg
     1441 gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac gtaaatgatc
     1501 agaacatgat gaatgaacag gaattcaacg tagagacatt agcaggcaca agaaaaatta
     1561 cggtgttgga tccctcacct ttttattgca gcgtagatat gggagtgcca gatgattctc
     1621 cggaaaaaat caaagctaat caaacttatc caataaaaaa ccaggaaata gagatcaatg
     1681 gtgaaaaagt aacgatcact agtttatttt taggaacgat tcatacagtt gtttttgttg
     1741 aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat catcctttgt
     1801 tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa ttgatcgtcc
     1861 gtacctatga aagaggagta ggttggacac ttgcttgcgg tacaggttgc tgtgcctctt
     1921 atgtggttgc taaggaaact gggaaaattt cagcagatca ggtagttgtt cacttggaac
     1981 aaggagactt gatgatctct ggtgaggatg cgatacaaat ggctggccca gcagttcatg
     2041 aatggacaag aatattggag gaatgaagat gataaaaggt tcgatcgtgg cgctgatcac
     2101 gccgatgaat gaagagggat cagtagatta tgctggtttg gaaaaattga tccaattcca
     2161 tttagatgaa caaacagatg gattattagt tttaggtaca acaggagaat cttctacgct
     2221 cacacaatct gaggaggagc agatccttca actgacagta aagaaagtgg ctggccgtgt
     2281 gccggtgatt gcaggagctg gaacgaataa tacgaaagaa acgattgaaa aagcgaagca
     2341 ttttgcttct ttgggggcag acgcactttt ggtcatcacc ccttactaca acaaaacaag
     2401 tgatgcagga ctagctgctc attttacagc aattgcagaa gcctcaccga ttccgcttat
     2461 tttgtataat gttccttcta gaacaggcat gtcgattcca atccatgtat tagtaaattt
     2521 agcagaacat cctaatatca ttggtttgaa agaagcctca ggagatatgg cttatgtgat
     2581 ggatgccgct cggttgattg gagaagaatt cttcctttac agtggaaatg atgatttgat
     2641 cctaccagta atgagtgtag gaggaagcgg tgtaataagt gtatgggcga atatccaacc
     2701 taaaatagtc catgaattag taaaagatac gcaggacgga agatggcagc aagcaaaaga
     2761 aaagcaatta aatgcacttg aactgatcca tgccttgttc agtgagacca atcctattcc
     2821 tgtcaaagca gccatgtcat tattagactt accttccgga cctctacgcc tgcctttagt
     2881 ttctctttca aaagagaaaa agaaacaatt agctcagctt ttgttaaaag aaaggacggt
     2941 tgaaaaatga aaatcggact gattggcagt ggacagatgg gaaatagagt cgaagaagtt
     3001 gcacaagaaa gaggagatac ggtccttctt ttacggacgg cacctaaaga agcaactacg
     3061 cttgctttta cggaactgcc agaactgctt attgattttt ctcatccaga taatttagag
     3121 aaaatccttt cgtatagctt atcatatcaa cttccgttag tcattggcac gacaggttat
     3181 acacaatcac agttccaaga aataaaagaa catgccaaac atgtacctgt gatgtatagt
     3241 gctaattttt cgttcggcat catggtgatg aatcaactaa tcaagcaagc agctgcaatg
     3301 ttgcaaggct ggcagatcga actgattgaa aaacatcata gtcgtaaaaa agatgcacca
     3361 tctggtactg caaatatgct gcttcaaaca atccaagaag tgcaaaagct gcagcccgta
     3421 tacaactggc aagggaaaaa aagagaagaa aaccaaattg gtgtacacag tattcgtgca
     3481 ggttctttac ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat atttacgatc
     3541 aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc agcggactgg
     3601 ttgaaagatc agtcaccagg ttattacaaa ttagaagatt tattaatgga caaaggggtg
     3661 taaaaaacgt ggaagctcaa gaaatcatcc gctacattca aacggcatct aaaaagacgc
     3721 ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt taaagaaagt aaagtttttg
     3781 gtgaaggaag cagcttcgtt gtttttggtg actatgagac gattgctccg gttttagaag
     3841 ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac agcgcagttc
     3901 ctttgctaga tattaaaaaa attaatgctc gtatcgaacc aggtgctatt atcagagatc
     3961 aagtgtcaat cggcaacaat gccgtgatca tgatgggagc gattatcaat attggtgcag
     4021 taatcggtga aaacacgatg attgatatgg gtgctgtctt aggtggacga gcgacagtgg
     4081 gcaaaaactg ccatattggt gcaggagctg tcttagcagg tgtgatcgaa ccagcatcgg
     4141 ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg attgttgaag
     4201 gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc agtggtttta gaagatgtgg
     4261 cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt gatgatcaga
     4321 caaaagaaaa tacagcattg attgctgcgt tacgcgaatt atagaggaga tggagaaaaa
     4381 tgcgcgaaat agatacctgt attgtggggg caaccggatt agtcggccaa acgatgctca
     4441 aagtgttaga agaatatgat tttccagtag gacgattgaa attattagct tcaaaacgtt
     4501 cagccggtaa agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa ttgacagaaa
     4561 cttcatttga aggatatgat ttagcgctat tttctgcagg tgcttctgta tccaaagagt
     4621 ttgcgccaat cgcacgaagt cagggactat ttgtcataga taattcctca gcatggcggg
     4681 aagattcgga tattccatta atcgttccag aggtcaatct ggaagactat acattgaatc
     4741 ggctgattgc aaatccaaat tgttcaacca ttcaagcggt acttccgctt aaagctttac
     4801 aagaagcctt tggtatccag cgagtcaatt attcgaccta tcaagcagtt tctggttccg
     4861 ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga cgaggaacca gttttgtatc
     4921 cttataatat tagccaaacg gtgattccgg aaatcgatca gcctttggaa aatgggtata
     4981 caaaagaaga acagaaaatg ataaatgaga cccgtaagat tctgcatcag cctgctttgc
     5041 cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc tctgtggcag
     5101 ttcaactgca aaaagagttt acgacggaag aaattagaga gtgtctcagc cagtttccag
     5161 ggattcaatt agaagatgat ctagcagcac ataaatatcc gacgagcatt cttgctagag
     5221 gaacagatat ggtttatgtt gggaggatca gaaaagacac ttctttagag aacggattgt
     5281 tgttctatac gactgccgat aacatacgca aaggagccgc tgcgaatgcc gtacagattg
     5341 ctgcagcttt aatgcagaaa atagcggagg tggcaagatg agtaatacgc tgaaagtagc
     5401 gaaattcggc ggaacatcga tgtctcatga agagcaattc aaaaaagtcc gtacaattgt
     5461 tctttcagat cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac gtacgtcaga
     5521 tgatgacaaa gtaacagact tactttattt gatttatgct taccttcagc atcatgttga
     5581 ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt caagtaagag atgttcttgc
     5641 cctgcagtta cctattgaag atgaactagc acgcatacag aaacaactgg aagacgaaaa
     5701 gataactgaa gattatttag taagtagagg tgaatatctg actgctcaac tgatggctga
     5761 gtatcttggt taccagttta ttgacgcaaa agacctgatt ttctttgact atgatgggaa
     5821 aatcgatcag gaaaaaacag cacaggaaat cacaaaagca tttaaaagat atccgaaaat
     5881 ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat tgggccgtgg
     5941 gggaagcgac attaccggcg cgatactggc gaagatgttg caagcggaaa gttatgagaa
     6001 ttggacggat gtttcaggaa tcatgatggc tgatccgcga atcatcgatc atcctaaaat
     6061 catcgaggaa atctctttta gagaactgag agagatggct tatatggggg ctaacgttct
     6121 tcatgaagca gctgttttcc cagttcaaga ggctgatatc cccatccaaa taaaaaatac
     6181 caatgatcct ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa aggaaaatgg
     6241 actgacagga atagctggac gcaaggattt tctttccatc actttattca aacgtcatat
     6301 gtccgatgaa atcgggttta tctggaaagc aatgagcatt tttgctaaac atgagatatc
     6361 aatcgaacat attccttcag ggatcgacaa tatcggagtg atcgtctcag cagaagcgat
     6421 tgccgataag ttatttctca tcacgaaaga attaaaagaa acacttggcg tggaagaaat
     6481 cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg ggtccgcata aagaattgat
     6541 tggattatct gggaaagtgt tatcgatatt gaacaaatta gaaattcgca catcgatctt
     6601 atcacaagga gcacaggagt tgaatctgat catcggtgta ccgaataatc aatatgaaac
     6661 agtagtgaag ggtatctatg aagggatggt gaatacggat gctgaaagaa tatcgaatgg
     6721 cactccatca aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa tatttatatg
     6781 aacaagtaaa aaacagcggg gccgttatcc atgaaatcgg cgaaacagga ctactccttt
     6841 attttgataa tcagcaagaa gaaacgattg cttttcgaac agatatcgat gcactgccag
     6901 tcaaagaaga aacgaatctc tcttttgctt ctaagcatga aggttatatg catgcatgtg
     6961 gtcatgacgg acatatggcg atgcttttag ggttgactga ctttataact aaacacattg
     7021 aggatcttat ttataatgta gtactgatct atcagccatc agaagaaatt tccggtggcg
     7081 cacaaagcgt tattcagtca ggattgttgg ataaatatga agtacaagca atatttggct
     7141 ttcatctatg gcctggacta gctaagggag aagttttctc tcgaccagga cctttgatgg
     7201 cacaaagcag tgaaacagac attatcatta aaggccagtc tgcacatatt gcttctagtg
     7261 aaaaaggaat cgatagtctg gaggcagctg tacgattttt gaagaatgtt tatgattttg
     7321 accggcaatt accggcagag tggaatcatc taatgaaatt tggccaaatc caaggtggaa
     7381 cgatccgcaa tgttttagct ggagaagtta ttgtatccgg aagtatccgt tcttacagtt
     7441 cagatatgca aaattatttg aagaggggat tagaccattt agcagaacag tttcactcta
     7501 cagctactat ttcttttcgc tataatgacg gctatccagc tgtaatcaac catagaggct
     7561 tatatgcagc actgttagat tcaaacaaac tacatgaatt agatgctcca gttcttcaag
     7621 ccgaagattt tggcatatat acgcaacaat atccatgtgt atttttcttt ttaggtgtcg
     7681 gtgatacccc tgctttacat gatccgacgt tcgattttga tatggatgtt ttagaaaatg
     7741 gtgtagaatg gtacaaaacg attcttttta cagaaaatct tcttgtaaaa atcgatagca
     7801 gttcaaaaga ggagcgagta aataatgatg aatttatcca atcgatttag tcctaggctt
     7861 tctagaatag aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc gataccagat
     7921 gtgatcaaac tcaccctggg agaaccggat tttccaactc ctgaacatgt caagcaagca
     7981 ggaatagcag cgattgaaga agacttttct cactataccg gaatgagagg attagaggaa
     8041 ctgcgggaag cggcttgtat atttcagcaa cagcgttatg gattaactta tgatccgcaa
     8101 acggaagttt tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt gttatctgtt
     8161 ttggaagaag gagacaaggt tcttattcca gcgcctgctt attcaggata tcagccatta
     8221 gtggaactag ctggtgctga attgatcccg atagatactt ctgacacagg gtttgtctgt
     8281 cagccggaac aatttgaacg agcatttgaa caatatggat cagctgtcaa agcagtcatt
     8341 ttaaactatc ctaacaatcc tactgggacg acattgtctg caaaacaatt gaaagcaatc
     8401 gctgaggttt taaaaaaata tccggtattt gttataagtg atgaagtata tgctgaattg
     8461 acttatagtg gaacgcatat gtccatagct acttatcttc cagaacaaac aatcgtgata
     8521 agcgggttat ccaagtcaca tgcgatgact ggttggcgag tgggctttat ttttgctcaa
     8581 aaagccctta ttgacgaact aattaaagtc catcaatatc tggtgactgc cgctacaacg
     8641 atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg tgaaagatag cgagaatatg
     8701 aaagagcaat atcaaacacg aagagactat ctggtccagc aattggcgcc tttaggattt
     8761 gaagtgaccc agccaaacgg tgcattttac ttgttttgca aactcccagc tgccattcaa
     8821 actgactctt ggcagttttg tgtagaatta gcggaacagg caaaagttgc gtgtatccca
     8881 ggaattgcat ttggcccaga aggagaaggt tatatccgga tttcttatgc tagcagcatg
     8941 gaaaaattac atgaggcctg caaacggatc aaagactttt taacagaaca gtgattcata
     9001 gattttatca aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag acgattgttt
     9061 tttaaattta gttcaaatga acgcgtttct tttgattcat aactaatgta atgttaagtt
     9121 taaattgttt agaatgattt taaaagaata ttcaaggagg tttcatcaat ggaaaaaatc
     9181 aaagttgtca ttgtaggtgc atctcatgga gggcatcaat cgatcttgga actattgaca
     9241 cgatacggtg aaaatgtaga tatcacgcta tttgaagcag gagactatat ttccttcatg
     9301 tcatgtggta tggagctgta tttagaagac aaggtaacag atgtaaatga tgtacgtaat
     9361 ttcagaccag aaaactttcc gcaatccaat gttcatattt taaataatca cgaagtaaaa
     9421 acgatcgatg ccgataaaaa aacagtaacg gttactagcg catcagataa cgaaacaaaa
     9481 gaatatcctt atgacaaatt gattttgagt tctggtgtca aaccaaacaa tctgccagta
     9541 ccaggagcag atctagaaaa tgtctacttg atgcgtggat acaattgggc aaccaaaatc
     9601 aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc gggttacatt
     9661 gggatcgaag cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt gcttgatgtg
     9721 atcgatagac ctttaggcac ttatttagat gctgagatga cagatatttt agaacaacac
     9781 ctaaatgaaa acggtattga agttttgaca aatgcaaaaa tcgaagcttt tactggtaac
     9841 ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct agtcattcaa
     9901 gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt agatgaacgt
     9961 ggctggatcc aaacaaatga atacttgcaa acgaatcttc cagatgtgta tgcagtagga
    10021 gatgctacac ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc tttagcgact
    10081 gttgcacgta gagaagcacg ttatgtagta aaacatcttt ttgaagaagt accaaacaaa
    10141 ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct ttgactatca ttttgcagca
    10201 agcggattga atagctttac agcagcaaaa gcaggagtag atgtacgagt gtcttattat
    10261 gaagatacgt tacgaccaaa atatgtacca gaagaacaag gaaatccaaa agtgtccatc
    10321 caattattct cggatccaat gacccatcaa ttgctaggag gagcagtatt atctacctat
    10381 gatgttacag gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact gactttagaa
    10441 gaactagcag aagctgactt cttcttccag ccaggctttg accgacaatg gagcatgttg
    10501 aatctagccg ctcaacaagc gttaggagaa gaaccatttg tagaataaga aaacgaaata
    10561 tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag ccaagtaaaa
    10621 acagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca
    10681 ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc aaaagttaga
    10741 ccaaaaaatc taacttttgg aggtcggtat tttttatggt taaatatact tttgaactta
    10801 aattaaagat cgttcatgat tatttggatg gcaaaggcga ttggaccgta aaaatccaga
    10861 tcaagagatt gaagcggaaa tgcttaaaat tcgggagaag cacaaggatt atggctgttt
    10921 acggatgacc aatgaactaa gaaaccgcgg tttctctatc aataaaaaga aagttcagcg
    10981 cttgatcaag aagctaggaa taaaagtaac ggcatacacg cgtaaatcac gtcgctacaa
    11041 ttcttatcga ggaaaagt
//
LOCUS       DACDSY010000095        11030 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDSY010000095 DACDSY010000000
VERSION     DACDSY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.096"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            19..747
                     /locus_tag="IWK44_002028"
     CDS             19..747
                     /locus_tag="IWK44_002028"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP9550527.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            747..1001
                     /gene="purS"
                     /locus_tag="IWK44_002029"
     CDS             747..1001
                     /gene="purS"
                     /locus_tag="IWK44_002029"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP9550528.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1003..1674
                     /gene="purQ"
                     /locus_tag="IWK44_002030"
     CDS             1003..1674
                     /gene="purQ"
                     /locus_tag="IWK44_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP9550529.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1675..3897
                     /gene="purL"
                     /locus_tag="IWK44_002031"
     CDS             1675..3897
                     /gene="purL"
                     /locus_tag="IWK44_002031"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP9550530.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3882..5321
                     /locus_tag="IWK44_002032"
     CDS             3882..5321
                     /locus_tag="IWK44_002032"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP9550531.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5353..6396
                     /locus_tag="IWK44_002033"
     CDS             5353..6396
                     /locus_tag="IWK44_002033"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP9550532.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            6393..6971
                     /gene="purN"
                     /locus_tag="IWK44_002034"
     CDS             6393..6971
                     /gene="purN"
                     /locus_tag="IWK44_002034"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP9550533.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            6968..8503
                     /gene="purH"
                     /locus_tag="IWK44_002035"
     CDS             6968..8503
                     /gene="purH"
                     /locus_tag="IWK44_002035"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP9550534.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8519..9793
                     /gene="purD"
                     /locus_tag="IWK44_002036"
     CDS             8519..9793
                     /gene="purD"
                     /locus_tag="IWK44_002036"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP9550535.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9915..10829
                     /locus_tag="IWK44_002037"
     CDS             9915..10829
                     /locus_tag="IWK44_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP9550536.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
BASE COUNT         3617 a         1864 c         2628 g         2921 t
ORIGIN      
        1 taaaaaggag agacgtacat ggagaagaaa gaacttttgt atgaaggaaa agccaaaaaa
       61 atatatctta cttctgatga gaacttgatt tggatagaat atttagatca agcaactgcg
      121 ttgaatggcg cgaggaaaga ccagataagt ggaaaaggag aattgaataa tcaaatcaca
      181 agtgcaattt tccgttatct gactcaaaga ggaataaaaa atcattttat tgaacaattg
      241 tctaaaaccg aacaattagc aaaaaaagta caaattatcc ctctggaagt ggttatccga
      301 aatattgcag caggaagttt ctcaaaacgt ctaggtgttc ccgaaggtaa agaattcatc
      361 aaaccaatca ttgaattcta ctataaggat gaccagttgg atgatccatt tatcaatgaa
      421 gagcatatct atttcttaca acttgcaaaa ccggaagaaa tcagtgagct gaaagcccaa
      481 gcgttggcta ttaatgaaat tttgaccgaa ttatttgcta aggtcggttt ggttttagta
      541 gatttcaagc tagaatttgg ccggtcgaat ggacaaattt tgcttgcgga tgaagtaaca
      601 ccggatactt gccgattatg ggatgaacgt acgaatgaac atatggacaa agacgtttac
      661 cgcagagatc taggtgattt gatcccagtc tatcaagaag tatataaccg tctatcggaa
      721 ctaactgaag aaaaggagcg agtatgatgt atttcgtaaa agtatatgta acctataaag
      781 attcagtgtt agatcctcaa ggggaggcag taaaaaaagc agtccatcgt atgggatatg
      841 aaacaatcga agatatccga ataggaaaat actttgagat acaacttgct gatgcagaag
      901 aaccaatcga tacagtgatt gaaacgattt gtgacaagct gcttgcaaat gtaaatatgg
      961 aaacataccg atatgaaatc aaaaaaatgg aggaagtata acatgaaatt tgcggtcatc
     1021 gtttttccag gttcaaattg tgacgcagat atgttatggg cagttcgcga gatcatgggc
     1081 gctgatgccg aatttgtacg gcatgatgag tcctctttag aaggatttga tggggtttta
     1141 ctgccgggag gattctccta tggtgactat ttgagatgcg gtgcgattgc tcggttttct
     1201 acaatcatgg aagaagtgat tcgttttgct gaagaaggga aaccagtatt cggtacatgc
     1261 aatggattcc agatattgac agaagcagga cttttgccag gtgtactgag acgaaatgag
     1321 tctttgcatt ttatttgcaa aactgtacca ttgaaagtca accccaagac ggcctttact
     1381 tcagagtatg aagccgacga aataatctat cttccagttg ctcatgggga aggaaattat
     1441 ttctgtgacg aagaaacact tcaagaacta aaaaaaaata atcagattgc ctttacttat
     1501 aatgacaaca tcaacggcag cgtggaaaac attgcaggga ttacgaacaa agcagggaat
     1561 gtgttaggaa tgatgcctca tccagaacgt gcagtagaag acttgctagg atcagaagat
     1621 gggaaacggt tctttgcttc cattttaaaa aacttcggaa aggtgaccgt atagatgaaa
     1681 acattgaaag aaccgacagc tgaagagatc aagaaaaaaa gaatctatgc cgaatgggga
     1741 ttgactgatg aagaatatcg aatgatcgaa gaagatatct taggaagaat gcctaattat
     1801 acggaaactg gactgttttc tgttatgtgg agcgaacatt gttcctataa aaattctaaa
     1861 ccggttctac gcaaatttcc tactagtgga cctcatgtgt tgcaaggccc tggagaaggc
     1921 gcaggcatcg tcgacatcgg tgatggacta gcagttgtct tcaaagcaga aagtcacaat
     1981 catccttcag ccgtggagcc ttatgaagga gctgcgacag gagttggcgg aatcatcaga
     2041 gatattttca gcatgggggc tcgtccaatt gcgttgctag attcattgag gttcggcgaa
     2101 ttagataatc caagaacgaa atatttacta gaagaagtag ttgcaggtat cggcggatac
     2161 ggcaattgta tcggcatacc gactgtaggc ggcgaaattg catttgatcc ttgttatgaa
     2221 ggaaacccat tagtcaatgc aatgtgcgta ggtttgattg aacacaaaca tatccagaaa
     2281 gggcaagccg ctggcgtcgg taattcgatc atgtatgtag gtgcaaaaac aggtcgcgac
     2341 ggtatccacg gagcaacttt tgcctcagaa gaatttaatc aagaagaaga acagcaacgt
     2401 tctgctgtac aagtaggcga tccttttatg gaaaagctgc tgcttgaagc gtgtttggaa
     2461 ttaatcttgc atcattcgga ttctctaata ggtatacagg atatgggagc agcaggactt
     2521 gtttcatcta gtgcggaaat ggcttcaaaa gctggctcag gacttatatt gacattagat
     2581 gaagtcccac aaagagaaac tgggatgaca ccttatgaaa tgatgttatc ggaatcacaa
     2641 gaacggatgt tgatttgtgt taaatcagga gaggaagaaa aaatccaaga actatttcag
     2701 aagtatgatc tagatgcagt cactatcggt aaagtaacag acgacgggca atatcggcta
     2761 tatcatcaag gagaagaagt agcaaatctg ccagtcgatg cattagctga agatgcacca
     2821 gtttatcata aagaaatgaa agaaccagct cgaatcgtag aatttcagca gttagctcct
     2881 tatcagccag ttatcgaaga acctggacaa gtattgcttg atctgctgca gcaaccgacg
     2941 attgcttcaa aacgttcaat ctacgaaact tatgattcac aagtacaaac caatacagtt
     3001 gtccgtccag gaagcgatgc tgctgtgatg agggttagag ggacgaataa agcgttagct
     3061 atgaccacag actgcaacgc gcgctatctg tatttggatc caaaaatcgg aggacaaata
     3121 gcagtggcag aagctgctag aaatattatt gctagcggtg gtaagccctt ggcgatcacc
     3181 gactgcctga actatggttc tcctgataaa ccagaagttt tttgggaatt atcgacttca
     3241 gctgacggaa ttgctgctgc gtgtgaaacg ttaggaactc ctgttatttc aggaaatgtc
     3301 tctttatata atgaaacgga tggtcaagca atctatccaa caccaatgat tggtatggtc
     3361 ggattgattg aggatcataa acatatcact actcaggaat tcaaaaaatc aggggatcta
     3421 atctatatat taggtaagac gtttgcagat tttgatggca gtgaattaca aaagatgcag
     3481 cttggaagaa tcgaaggtgt gatccgaaac tttgatctat ccattgaaaa gagaaaccaa
     3541 gagttagtat tgacagccat ccaaaacgga ttgattgaaa gtgctcatga ctgttcagaa
     3601 ggtggcttgg caattgcact ggcagaatca gctttcaaac atcagctagg gatatctgta
     3661 cagtttgaat tgtcctctgc tcaattattt gcagaaactc aatcaagatt tgtgttgaca
     3721 gtcgcaccgg aaaataagac acgattcgaa gaaatgatgg gagatgctgc tgttttagca
     3781 ggtaaggtga cggatgaagc gataatcgaa atttcagcaa ctgacggtca gatcaaaata
     3841 gaaactgcag tagcaagaaa gtgctgggag gatgcaatcg tatgtcttat gaagtgaaga
     3901 gtttaaatga agaatgcggg atcttcggtg tttggggtca tccagacgcc gctagtgtgg
     3961 catacttcgg gctccatagt cttcagcatc gaggacaaga aggtgcaggt atcgtgtcca
     4021 atgatcgagg aaagcttaag gggcatcgag atttggggct gctttctgag gtattcaaag
     4081 atcaaagaaa attgcagcag ttaacaggag aagctgcaat cggacatgta cgttatgcaa
     4141 cagcaggcaa tggaagtgtg gacaatattc agccttttct attcaaattt catgatcagt
     4201 cgatcggctt agcgcataat ggtaacttga tcaatgctcg ttcccttaga aaagagttag
     4261 aggaaaatgg ggcgatcttc cattctaact ccgatacgga gatattgatg catttgatcc
     4321 gacgaagtga agaaacagat tttttaaata aattaaaaag tgctttgaat gaagtcaaag
     4381 gtggctttgc ctatttatta atgacagaaa cggcaatgat tgccgctttg gacccaaatg
     4441 gttttcgtcc attagcaatc ggacaaatga aaaacggagc atacgtgatt gcctcggaaa
     4501 cttgtgcatt agaagttatt ggggcacgtt ttataagaga tgtccagcct ggtgagatag
     4561 tgatcatcaa tgacgaagga atccagattg attgctttac gaaggacacg cagctttcta
     4621 tttgttcgat ggaatatatc tattttgcca gaccagattc aaacatcgca gggatcaatg
     4681 tgcatactgc acggaaaaat atgggacgga atttagcgaa agaatcgcca gtaaaagcag
     4741 atatggtcgt tggcgtacct aattcttctt tgtcagccgc aagcggttat gcggaagcta
     4801 gtggaatccc ttatgaaatc ggattagtaa aaaatcaata cattgcccga acatttatcc
     4861 agccaacgcc tgagctaaga gagcaaggtg ttcgaatgaa attatctgct gtacgaggag
     4921 tagtggaagg taaaaaggtg attatggtcg atgattcaat tgttcggggt acgacaagca
     4981 gacggatcgt ccatcttttg aaagaagcag gggcaaaaga agtccatgtg aggattgctt
     5041 ctcctccttt gaaatatcca tgtttttacg ggattgatat tcaaacgcgt aaagaactca
     5101 ttgcggcaaa tcacacgatt gatgaaatca aagaatgtat cggtgcagat tctttaggtt
     5161 ttttaagtga agagggtctg atcgaatcga tcggtctgga aaaagatgct ccgtattcag
     5221 gattgtgtat ggcttatttc aacggagatt atccgacgcc gttatatgac tatgaagaaa
     5281 aatatcagga atcactaaaa gaaaaagtat cgttctttta aaagcagacc aagaagggag
     5341 tatatgaatt caatggcaaa tgcatacgca aaagctggcg tagatgtcga agctggatat
     5401 gaagtagtag aacggatcaa gaagcatgtc aaaaaaacgg aacgtttagg tatgatgggc
     5461 gctataggag gattcggcgg ttgttttgat ttatcggtct ttcaattaaa agagccagtc
     5521 cttgtctcgg ggaccgatgg cgtggggacg aaattagcag tggcaatcga agcagacaaa
     5581 cacgatacaa tcgggatcga ctgcgtagcg atgtgtgtca atgatatctt agcacaaggc
     5641 gcagagccgt tatatttctt agactactta gcaacgggga aaaatgatcc gttacgtttg
     5701 gaaaatgtag taaaaggtgt agcagatgga tgtgttcaag gaaatatggc gctgattggc
     5761 ggagaaacag ctgaaatgcc tggtatgtac tcaggtaatg attatgatct agccggattt
     5821 gctgtaggga tagctgaaaa atctcaatta ttgacaggag aaacgatcaa cccaggagat
     5881 gtgttgcttg gaatagcatc tagcgggatt cattcaaatg ggtactctct tgttcgcaaa
     5941 attttctttg agcagcagca gttttcttta tcatcaaaac tgccagaatt gaaaaaaaca
     6001 cttggcgaag aattgcttga gccaacgaaa atctatgttt cttccttgct tccgctgatc
     6061 aaagaaaaga aaataaaagg agctgcacat atcactggtg gagggtttgt agaaaatatc
     6121 ccgcgaatgt taccagaatc tatggctgca caaatcaaac ttggctcatg gccgattctt
     6181 ccaatattcg gctgtttaca aaaatatggt gaaattcctg agatggagat gtatgaaatc
     6241 ttcaatatgg gcatcggtat ggtcctggca gtcgaagaat cgaaagtaaa agaaatagta
     6301 cacgaaattg agcaagctgg cgaaaaagtt tatgaaatcg ggaaagtgat cgccaaagaa
     6361 aaacatcgtg tggtgtttat aggagaagaa cgatgagaat cgctgttttt gcttctggaa
     6421 acggaagcaa ctttcaagcg ctagccgatt atctatcgaa aaaaggattg gaatcttcga
     6481 ttgactggtt gttttgtgat cagcctgaag catatgtttt gaaacgtgca actgcgttga
     6541 gtgtaccggc agattgtttt tcacctaaag aatttgattc aaaaaaagag tatgaagaag
     6601 caattttgca taaattgaaa gaaaaaaaga tcgatttgat cgtattagct ggatatatgc
     6661 gaatcattgg accggtattg ctgaaaaatt atgataaaag aatcatcaat atccatccgt
     6721 ctcttttacc tgccttccct ggtttgcatg gcatacgtga tgcctttgaa gctggagtaa
     6781 aagaaacggg tgtcacaatt cattatatcg accaaggtgt agatactgga ccaatcatcc
     6841 ggcaggaaaa agtgagaatt gaacaggagg acacgttcga ttctttagaa gaaaaaatcc
     6901 atagagtaga gcatcgaatc tatccagaag tgattagtga aattattgaa aatggaggct
     6961 atctgaaatg acaagagcat tgatcagtgt atcagataaa actgggattg tagcattcgc
     7021 ccaaggtttg attgaacaag gagtagaaat catttcaaca ggaggaacaa agcatgcgtt
     7081 ggataaagct gggatcaaaa cgctttcgat cgaagaagtg acagggtttc ctgaaatgat
     7141 ggatggccgt gtcaaaacac ttcatcctaa aatccatggt ggtcttttag gaagacgcga
     7201 tctccctagc catatggagg caatggaaac ggaaaatatc caaccaatcg actttgtttg
     7261 tgtcaattta tatcctttca aagaaacgat tatgaaacca gatgtcgaaa tagcagaagc
     7321 tattgaaaac attgatatcg gtggtcctag tatgctccgt agtgcagcaa aaaaccatga
     7381 atttgtcacc tctgttgttg atccatctga ctatgaagaa gtactttcag aattacgtca
     7441 gtatgggcat acttcacttt ctttcagaca aaaactagcg gcaaaagtct ttcgtcatac
     7501 tgcggcatat gatgcactga tcgctcaata cttaacggat tgggtggaag aaaaagaacc
     7561 ggaaaaattg acacttacgt atgaacgaaa acaaacgctt cgttacggtg aaaatagcca
     7621 tcaagccgct gccttttatc agtccgctct tctggaaaaa tattcgatcg cagcagcaaa
     7681 acaactccat gggaaagaac tttcttataa taatatccga gatgcagatg ctgcgctacg
     7741 gatcatgaag gaatatgacg agccaacggt agtcgccttg aaacacatga atccttgtgg
     7801 tattgggaca gcatctacga ttttagaagc gtgggaagca gcgtatgaag cagatcctgt
     7861 ttcaatcttt ggtgggatca tcgttttgaa tcgagaagta gatctgccaa cagcagaaca
     7921 gatgcataag ctgtttctag aaatcatcat tgccccttct tatacacaag aagctttgga
     7981 attactgcaa aaaaagaaaa acatccgatt actgacagta gactttgcgc aggaagaaaa
     8041 tcaaaatcga gaagaaacgg tctcagtctt aggtggactt cttgtacaag atcaagatct
     8101 ggcaatagaa aatgaagaag agtggaaagt cgtgacaaaa cgtaagccga ctccagcaga
     8161 agcaaaagca ttagcgtttg cttggagagc tgtcaaacat gtgaaatcta atgcgatcgt
     8221 actggcaaac gaacgtcaga cagtaggaat cggagccggg cagatgaatc gagtcggttc
     8281 agtaaaaatt gcgattgaac aagcgcaaga gaaaatggaa ggtgcagttt tagctagcga
     8341 tgcttatttt ccaatggatg attcggttga atatgcagcc aaacatggta taaaagcaat
     8401 cattcagcca ggaggaagca tcaaagacca agattctatt gatatggcga ataaatacga
     8461 tctagcaatg gtctttactg atgttcggca ttttagacat tagagaggag tcggataaat
     8521 gaacattttg gtgatcggtg caggtggtcg tgaacatgca ataagtaaaa aacttttagc
     8581 cagtccgaag gtggatacgg tgtattgtgc gccaggaaat ccggggatga caaaagatgg
     8641 tatcaattgt gtcgctatca gtgagcgaga tcattcagcg ttaatccatt ttgttaagaa
     8701 agaggctatt gattggagta tcgttggtcc tgaagttcct ttgttaaatg ggatcgtcga
     8761 tgattttatg gcggaaggat tgaaaatctt cggtccgaca aaagcagcag ctctgatcga
     8821 aggttcgaaa agttttgcta aacaattaat ggatacttat caaatcccga cagctaaatc
     8881 cagaagtttt gaatgttacg aaaaagcagc agcgtatatc aaagaaaatg gtgcgccaat
     8941 cgtagtgaaa gcggacggct tagcagctgg gaaaggtgta gtcgtggcta ccacagtgaa
     9001 ggaagcgctg gatgcggcag aacaaatgtt gaaccagcat cgatttgggg atagtagcaa
     9061 aaaaatcgtc gtagaagagt ttttggcagg agaagaattc tcccttttgg cgtttgtccg
     9121 taataccaag gtgtatccga tggtcatttc gcaagatcat aagcgggcat ttgataacga
     9181 taaagggcca aatacaggcg gaatgggtgc ttattcacca gtaccgcaaa tttcaaaaaa
     9241 gatgattgaa aaagctgtgg aagaagtttt aaaaccagcg gctttgggaa tgaaagaact
     9301 aggacgctct ttcacgggtg ttttatatgc gggtttgatt gctacgaagg aaggaccaaa
     9361 agtgatcgaa ttcaatgcgc gtttcggtga tcctgaaacg caagtcgttc tttctcggtt
     9421 gaagagtgac tttgcgcaag taatcgatga tttattagag aatcgtacag tagagttaaa
     9481 gtggcagcaa gaaggataca atgtaggcgt tgtggttgca gctgccggtt accccgaaga
     9541 atatgagaca ggaatggttc taccagattt tctggaagaa gagctttctg tttactatgc
     9601 tggagtttcc tcaaataacg gcaaactagt tggagcagga ggacgtcttt atttagtaga
     9661 agcatacggt gagacgatca aagaagcaca gaaaaaaatt tacaccgcgt taatggataa
     9721 aacgacagat ggaacatttt atcgtacaga tattggagaa aaagctgcca atagcgaact
     9781 gatcgtgaag taaatcagct gcttccattt ataaaaaata caacaaacat aatcggaagc
     9841 gccgatttta gtgaagcctt ttcataaaga gtgtggtaca cttttgttgt gtatataaat
     9901 aaggaggagt tcagatgaaa aaaattcaat taggtacttc tgatcagaaa gcagctgcag
     9961 tgatcttagg ctgtatgcgt atcaacaatg cggaagatcc agtaaaagtg attgagacag
    10021 cttatgaaaa cggcattgat ttttttgacc atgcagatat ttatggaaac ggtgaatgtg
    10081 aagaaatctt tgcagatgct ctagcaaaaa catcaatcaa acgagaagat ctttttattc
    10141 aaacaaaatg tggaatcgtt ccaggtgtga tgtttgattt ttctaaaaaa catatcattg
    10201 aatctgtaga aaaaagtttg acccgtctga aaatggatta tgtggatgct ttacttcttc
    10261 atcgtccaga tacattggta gaaccagagg aagtagctga agcgtttgat gaattggaac
    10321 ggtcaggaaa agttcgttat tttggcgtca gcaatcaaaa accaatgcaa atcgaattgc
    10381 tgaaaaaata tgttcgtcaa ccattagtag ctaatcagct gcaatttggc ttgaaacata
    10441 ctggaatgat cgatcaaggg attcatgtca atatgacaga tgaagcaagt atcgatcatg
    10501 acggtagtgt acttgattat tcccgattgc atgatatgac gatccaagca tggtcaccat
    10561 accagtatgg attttttgaa ggtgtattta tcgggaacga aaaattccct gaattaaacc
    10621 aaacattaga agaactagca gctaaatatc aaacgacacc aactggcttg gcttctgctt
    10681 ggattttacg ccatccagcc aatatgcaag tgatcgccgg tacgatgaac gtagggcgaa
    10741 tcaaggaaat cgctcaagct tcagaaatta atatgtctcg ggaagattgg tacgaattgt
    10801 accgtgctgc tggaaacatt ttaccttaaa gattgtcata agcaggtcaa aagggctata
    10861 aattaaaaag acagaataac ttgaaagctg ttttcagctt cgagatattc tgtctttttt
    10921 tattgcaaat ggaaagagta tcattgtacg gatttcgttt attagttatt tttgttttta
    10981 aggaaaagct gtgtccgata ttcttttgga gaaatccctt gttttttctt
//
LOCUS       DACDSY010000096        10931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDSY010000096 DACDSY010000000
VERSION     DACDSY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.097"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..288)
                     /locus_tag="IWK44_002038"
     CDS             complement(<1..288)
                     /locus_tag="IWK44_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9550537.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAEN"
     gene            430..1548
                     /locus_tag="IWK44_002039"
     CDS             430..1548
                     /locus_tag="IWK44_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9550538.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1676..3052
                     /locus_tag="IWK44_002040"
     CDS             1676..3052
                     /locus_tag="IWK44_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9550539.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3453..5033
                     /locus_tag="IWK44_002041"
     CDS             3453..5033
                     /locus_tag="IWK44_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP9550540.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5231..5454
                     /locus_tag="IWK44_002042"
                     /pseudo
     CDS             5231..5454
                     /locus_tag="IWK44_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5659..5979)
                     /locus_tag="IWK44_002043"
     CDS             complement(5659..5979)
                     /locus_tag="IWK44_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9550541.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6127..8361)
                     /locus_tag="IWK44_002044"
     CDS             complement(6127..8361)
                     /locus_tag="IWK44_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9550542.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8840..9106
                     /locus_tag="IWK44_002045"
     CDS             8840..9106
                     /locus_tag="IWK44_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP9550543.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9106..10833
                     /gene="ptsP"
                     /locus_tag="IWK44_002046"
     CDS             9106..10833
                     /gene="ptsP"
                     /locus_tag="IWK44_002046"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP9550544.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3480 a         1912 c         2216 g         3323 t
ORIGIN      
        1 gttttctgct tcttcaatca atatttttcc taacccttgg tgtctgtatt cagaaagaat
       61 cgccatccgc tgtaatttca tttttccgtt ttctaaaggc aaaagacgta cagttccttg
      121 cggttctttt ttatctgagt acaggacgaa gtggatacaa tgggcttcat ctttgtctat
      181 ttctcgagac aaaggcacac cctgttcgac tacaaaaact tgatgcctga tccgtacagc
      241 atctaagtaa atattgctca ttgtatcttt tgtttgtaca actttcatgg tctagccacc
      301 ttcctttcgt atagtatact ggaaaataaa acaaataaaa agtatggcaa gttttcaaaa
      361 ataaaaaaag aataaaagaa tttttaacga atataatata tactagtaag aaagaaagga
      421 ggaaatctat tgtttgaaaa acttagacag tctaaactaa tgttttggtc gcttgaatta
      481 ttgatcatag ctgcattgat catgatttca tcagaaataa actttatttt cgaaccgatc
      541 gggacatttt tttccacgtt atttgcacct gtactgattg caggctttct ttactattta
      601 ttgaatccta ttgtcaagct gttgatgaaa gcaaaaatca aaagaatatg ggctgtcgca
      661 attgtgctgc tccttttagt tgcagcaatt atttggattt tacttagtgt catcccaagt
      721 ctagtacagc aaatttcctc attggcatct aatatgcctg atttcatcaa acaggtagaa
      781 agctggttaa aagaggtagc cgaattgcct ttgttcaagg aagtagatat caataaatat
      841 tttgagcaat tagacatttc ttacgggaca atcattcagc aattccttag tggagtatcc
      901 aatagtttag gttcgatcgt tggaacgatc gcttcggcaa ccgtagtaat cattacagca
      961 ccattcatct tgttctatat gttgaaagac ggagaaaaac tagtgccaaa catccaacag
     1021 tttttcccag aaaaaagaag aaaccaaatt gtggagttat tggggcaact gaataaaaca
     1081 ttggctaatt acattagcgg acaagcaatc gaatgtttat tcgttggtac cttcacattt
     1141 ttaggatata tggtaattgg tgtggattat gctttcttat ttggggtcat tgcgggattg
     1201 acaaacctaa ttccatattt aggaccttat ctaggactag cacctgcatt tctagtaaca
     1261 gtcttcaatg atccggtaaa agcattactt tgttgtgtgg ttgtgctaat cgtccaacag
     1321 ttggatggga atatcattta tcctaatgta attgggaaat cactgaaaat ccatccatta
     1381 acgatcatta tcgttcttct agtagctgga aatattgccg gattgcttgg tatcttctta
     1441 ggtgttcctt tttacgcaat ctgcaagacg atcatttcct atataatcaa aatcgtcaaa
     1501 gaagacaagc aaaatgaaaa caagaagaaa attgctacga cctcgtaggt agttttagta
     1561 cacttgtttt tattgcaaag aaaattgctt tgtgatacca ttttaacgtg gctatagtgc
     1621 cacgtttttt tctatcgtaa actaaaaatt aattttataa ggagagaatg cgattatgaa
     1681 tgctgaccct gagagtcagt cgctgttagc gcagatttta ttattgatcg ttttgacatt
     1741 gatcaatgct tttttagcag cttccgaaat tgctgttgtt tcgatcaata agaatcgaat
     1801 cgagcaaaaa gctgatgaag gagatgtgaa atctcaaaag cttttaaaaa tattgcagaa
     1861 tccaaataat ttcctatcga caatccaagt aggaatcact ttggtcaaca ttttgtctgg
     1921 tgcttcacta gctaatactt tatcggaacg gttagctccg gtattaggtg gcggcacagc
     1981 ggcaaaaaat attgcaaata tcattgtgct ggctattttg acctatgtat cgattgtttt
     2041 tggagaattg taccctaaac gtattgcttt aaataaatca gaagaagtcg caaagttcac
     2101 ttcagggctg atacgcttga ttggtgtagt agctaagccc ttcgtctggc tgctttctgc
     2161 ttccacaagt ttattggcgc gtatcacgcc gatgactttt gatgatgaag attcaaaaat
     2221 gaccagagat gaaatgcgtt atatgcttga aaacgaaggc gtattgaata atgaagaatt
     2281 agaaatgctc caaggggttt tttctcttga tacgaaagtt gcccgagaag tcatggtccc
     2341 acgaacggat gcatttatga ttgatatcaa cgacactgtg gaagaaaatg tgaatgaagt
     2401 tttatccgaa aactattcaa gaatccctgt ttataacgaa gacaaagata aagtagtcgg
     2461 aatccttcat acaaagaatt tactaaaagc tgcccataaa ttcggctttg ataatttaga
     2521 tatcaagaaa atcatgcagg agcctttatt cgttcctgaa acggtcttta ttgatgatct
     2581 gttatatgaa atgaaaaaga cgcagaatca gatggctatt ttattagatg aatatggcgg
     2641 cgtcgttggg ttagcaacac tagaagacct tttagaagaa atcgtcggag aaatcgatga
     2701 tgaatcagat gaagttgaga atctttatga aaaaatcgat gaacacgaat atatcatcca
     2761 aggacgtatg ctgattgatg agttcaatga agcttttgac agtgatctgc atatgagtga
     2821 tgtagatacg atggctggtt atttgattac agcattaggc atgattccgg atgaaggcga
     2881 aaagttatcg tttgatgtcg ataatattac gctggtttct gaagaaatgg aaggttctcg
     2941 aatccttaaa atccgtgtca tttttcatga tccggaagaa acagaagcgg aacctgaaga
     3001 agaacgaaga tacttcagaa aagaattcga agatgacgag ccaagaagat aattggaaac
     3061 ttggttatga aagacgtgtt gcttgaataa acaagcaaca cgtcttttct tttagtcagc
     3121 tgacaaaaaa atatttgact cttatggttt ggtggctttt tcattcgctg aatccatgct
     3181 atactgattc agttgtagta gttgacaaag gttgtgaaaa agctatcctg cattaagtaa
     3241 taagagcagc caagcaaaaa cagcttttcg tatttttggc ttgggtgggc ttattgctga
     3301 taggctgtct ctagaacgcc gtttactcgg aaaaagagac tgctacacgg cttttgtcac
     3361 agccctatat gaaattgtct ttctttaaga gatcggacaa tctgtgccta gagaaaatat
     3421 aaataattga atggaagaaa tggagttagg ttatgaataa tccaaaatta aagcaacaag
     3481 tggacaatcg cagaactttt gcgatcattt cccatccgga tgctgggaaa acaacgatta
     3541 cagaacaatt actattgttt ggtggggcta tccgtcaagc cgggaccgta aaaggcaaaa
     3601 aaacaggtaa ttttgccaaa tcagactgga tggaaatcga gaaacaacgt gggatttctg
     3661 ttactagttc tgttatgcag tttgattatc aagggaaacg agtaaatatc ttggatacac
     3721 caggacatga agatttttct gaagatactt atcgtacttt gatggccgtg gatagtgcgg
     3781 tcatggtaat cgatagtgca aaagggatcg aagcacagac aaagaaatta ttccaagttg
     3841 taaaaaaacg tggcatcccg atatttactt ttatcaacaa actagatcgt gatggtcgtg
     3901 aaccattgga attgttagaa gaattagaag agctattaga tatcgaatct tatccgatga
     3961 attggccaat cggtatgggt aaagggctag aaggtcttta tgatatctac cataatcgtg
     4021 tagagtttta tcgtccggaa aattatcagg atgaacgatt agtagaattg gatgaagatg
     4081 gactgcttcc cgaaaatcat cctctaactg aaaattcctt atatgaacaa gtcttagaag
     4141 aagtagaact aatcaaagag gctggagatg catttaatca agaaaaaatt gctcgtggcg
     4201 atcagacacc agtatttttt ggttctgcat tgactaactt cggtgtacag acttttttag
     4261 agactttcgt tgaattggca ccagctcctt atggacacaa aacacaagat gatcagatgg
     4321 taagtcctta tgaggaagaa ttttcaggtt tcgtattcaa gatccaagca aatatgaatc
     4381 cagcccatcg tgaccgaatc gcttttgtcc gaatctgttc tggaacattc gaacgaggaa
     4441 tggatgtctg gttggaacgt acaaataaaa aactgaaatt aagcaatgtc acacaattta
     4501 tggcagattc gagagaaaac gtcgaaaaag cagtagcagg cgatatcatc ggtgtatatg
     4561 atacaggcaa ttatcagatt ggggatacat tgttcgaagg aaaactaaaa gttgcttatg
     4621 aagaactccc ttcatttact ccagagctgt ttatgaaagt gactgcaaaa aatgtcatga
     4681 aacaaaaatc cttccacaaa ggaatctatc aattagttca agaaggagcg attcagcttt
     4741 ataaaacata tctaacggaa gaatatatca ttggagctgt tggtcagcta caattcgaag
     4801 tattccaata tcgtatgtta aatgagtata acgccgaagt aatcatgtct ccaatgggac
     4861 acaaaatcgc tcgctggatc gatccagaag atttggatga gaagatgagt tcaagtcgca
     4921 atatcttggc tcgtgaccgt ttcgaccaac cgttgttctt attcgagaac caatttgcag
     4981 aacgctggtt tgcggataaa tatcccaatg taaaattaaa aagtttgatg taaagaagtc
     5041 ttgacacaag ctgccctgca tcaagtaata agaatagcca aacaaaaaca gcctctcata
     5101 ttttgtttag agccgtgacg gattttccgt gacggctctt tttactatat taaaatcata
     5161 gaaatgtcta atcaaaaaaa tataaaaatt attttgcgta catatgatta aagaaaggag
     5221 agtgtgacac atggaaacag aatggcaaag acgtgaaaga aaaaaacaga aaaagaagag
     5281 atgttacgat tatgtcgatt attagatgta aaagactggg acaaaaacga gcaaattgca
     5341 caaaaagtac gatcaatcat ccataaagag gaaaaagtta aaaataaaga cacgcgtaga
     5401 actgaaaatg aaaagagaaa agtacaaaag aaaagagaaa aatcaaaaag ctagaaagaa
     5461 aaagaggctg agacagaagc gtttaactcc aagaaataag ctggaattca caaaaatttg
     5521 aaaagcaatt ttcgtgaatt tcagcttatt tccgaaggag ttgcttctgc tttcgccgtt
     5581 tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctctttaatt
     5641 attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt ttggaatcga
     5701 gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt aaacggcgtc
     5761 tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct ggattttacg
     5821 tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga caatagtggt
     5881 atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct ggtttaatac
     5941 aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct cttttatgta
     6001 tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac agtattttga
     6061 cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca cgctataaaa
     6121 ctggatttat tcagccgttt cttcggctga ttctttagcc aaacgttctg gtttagaagt
     6181 aacgatgatt tttccatctt tatccagttt tgctttcaac tcatggatag aagggtgatc
     6241 aagataaaat tcagcgatac catcttcgat ttgttcttgg atcgtgcgtc gaagaggacg
     6301 agcacccata gatggatcat agcctaagtc aactaatttt tctttgacat tcgtaggaac
     6361 ttcgatgtga agttgctgtg ctgccagcat ttggttgaca tcgtctagca ttagtgtgac
     6421 gattgtcatc aagttttctt ttgataatgc actgaattcg atgatcccgt caaaacggtt
     6481 cagaaattct ggtgtaaagt agttgtttaa ttggttcaat acagaacgag taacaccttc
     6541 gcgtgccgca ccaaaaccaa cgtttgcttc tactttacca gttccggcat tacttgtcat
     6601 aatgatgatc gtatctttaa agctgaccgt tcttccttgt gcatctgtca atcgaccatc
     6661 atctaagatt tgcaagaaca tatgcaaaac atctggatga gctttttcta cttcatctaa
     6721 caagacaaga ctgtaaggat ttctccgtac tttttctgtt aattgaccag cttcttcgta
     6781 tccgacataa cctggaggag aaccaatcaa tttagagaca ctatgttttt ccatatattc
     6841 agacatatcg aaacgaatca ttgaatcttg tgaaccgaat agttcgtagg caagttgttt
     6901 cgcaagctct gttttaccta caccagttgg tccaacaaat aagaaggagc cgattggacg
     6961 attctgtttg tttaatccaa cacggtttcg gcgaatcgct ttcgctactc gatctacagc
     7021 gttatcttgt ccgatgacat gagctttcaa gtcatctgct aagtttttca gttgtgtctg
     7081 ttctttttct tttaattcac ccactggaat acctgtccgt tgttcaacga ttttttccat
     7141 atctttttcg gtaatcacag gtgtttcctc ttcagtgagc tgacgttctt tcattttttg
     7201 aagtttattg atctgatcgc gataataagc tgctttttcg aaatcttctt ctcgagaagc
     7261 taaaactttt tgttcttctg cttcttgcag ttttttctcg attgttttag gatcaacgat
     7321 ttggatcgtc aagtttttct tagaacctgt ttcatctagc aaatcgatgg ctttatccgg
     7381 caagaagcga tcttggacgt aacgattgga taaagttgcg gctgcttcga tggcctcatc
     7441 agtatatttt acatgatgat agtcttcata acgtttttgc aaacctttca aaatactgat
     7501 cgtttcttcg acagttggtt catctacccg tacaggttgc atacgacgtt ctaaagcagc
     7561 gtctttttcg atgatgcggt actcatttaa ggtagtagca ccaaccattt gcaattctcc
     7621 tcgagctaaa gctggtttta aaatattacc agcatccata tttccatcac cagcagaacc
     7681 agcaccgacg atttcgtgaa cttcatctat gaataggatc acattttctg cttgacggat
     7741 ctcttcaatc aatttttgca tacgttcttc aaattgtcct cgtataccag taccttggac
     7801 taaagatacg acatctaaac gaatgacttc tttgtccatt aatttttgcg gtacatcacc
     7861 atctacgatt ttttgtgcta aaccttcaac aactgctgtt ttaccaacac ctggttctcc
     7921 aatcaagaca ggattgttct tggttcggcg atttaaaatt tcaatgactc gtttgatttc
     7981 gtcatcgcgt ccgatcacag gatcgatatc accttgtcgt gcttgttctg tgatattgat
     8041 tccgtattct cccaataaac cttctgtatt gccttgaggt ggctgaccac cattgaagtt
     8101 gttgtttccg ccaaattgtg ttggtggtgt ttgctcataa tttccttgct gttgcatttg
     8161 gcgagacatg gaacggaaaa gatcgtccaa actgccaaat ccaaatggat cattttgggc
     8221 catattagtc agacctccat tttgttgatt tttgattttt tgataacagc tctgacagta
     8281 atcgagttgt gtccgctgac cattgacagt ggcatacaag tgaatcgttg cctcattctt
     8341 gccgcagttt tgacaaagca tgaatcattc acgtcctttc acaaaaatgg gtacatccat
     8401 attttacatc tcgtttttca tgaggtaaaa gaataacccc tagtttttta agagatttat
     8461 gaggtagaaa aggtgaaatc tctattgacc attcctgacc tttgactctt attatactaa
     8521 aaataaaaag ggaagcaagt aattcgtttc atatgaaaac aaagagggtc aaaggaaaga
     8581 atgataaacg tcaggagaag agaaagcgac aagaaaaagg aaataaacac gtttgttata
     8641 aaatgtgaag aaaaggatga aaaagaatcc ggaataattt gaatactttt atctgaaatt
     8701 gaatgaaaac tgttgtatct aacgaaaaaa aatgttaatc tttacagatg aaagggtggc
     8761 atttgttagg attctctgaa ttactaaaaa agaaaaccct cacataaaaa caacactcac
     8821 ttaaaggaga ccgattaata tggaaaagaa agaatttcac gtagtagcag aaactgggat
     8881 ccacgctcgt ccagctacac tattagtaca aacagcaagc aaatttaact ctgatgtaaa
     8941 cttagagtat aaaggtaaat cagtaaactt gaaatcaatc atgggcgtta tgtctttagg
     9001 tgtaggtcaa ggttctgatg ttactattac tgcagaaggt gctgacgaag ctgatgcaat
     9061 ggcagcaatc gttgaaacaa tgaagaaaga aggcttatct gaataatggt tgaaatgcta
     9121 aaagggattg ccgctagtga cggtgtagcc gttgcaaaag cttacctgct agttcaaccg
     9181 gatttaacat tcagtaaggc aacagtagaa gatactgcgg ctgaagaagc tcgtttggat
     9241 gctgcattag ctaaatctac tgaagaattg cagcaaattc gtgaaaaagc agcgcaaagc
     9301 ttaggtgaag cagaagcgca agtatttgat gcccatttga tggttctttc agatccagaa
     9361 atggtaggtc aaatcaagca aaatattaaa gacaacagcg taaatgctga atcagcgcta
     9421 aaagaagtaa cggatatgta tatcggtatg ttcgaagcaa tggaagataa tgcttatatg
     9481 caagagcgtg ctgcagatat tcgtgacgtt gctaaacgta ttttagcgca cttattaggc
     9541 gttactttac caaatccatc aatgatcaac gaagaagtgg tcgttgttgc acatgacttg
     9601 actccaagtg atacagcaca attagatcgc aattttgtaa aagcgtttgt aacagacatt
     9661 ggtggacgta cttcacattc tgctatcatg gcacgttctt tagaaattcc agcaatcgtt
     9721 gggacaaaag aaattactgc taaagtcaaa gaaggcgata ttttggctgt caacgggatc
     9781 gaaggcgatg taatcatcga tcctacagat gaacaaaaag ctgaatttga aaaagcaggt
     9841 gcagattacg cagcacaaaa agcagaatgg gaaaaattaa aaaatgcaga aactgtcaca
     9901 gctgacggca agcattttga attagctgca aacatcggaa caccaaaaga tctagttggt
     9961 gtccataata atggtggaga agcagttggt ctgtaccgta ctgaattcct gtatatggat
    10021 tctccagact tcccaacaga agatgaccaa tatgaagctt acaaagctgt attagaaggt
    10081 atggaaggaa aacctgtagt tgttcgtaca atggacatcg gtggagataa agaactacca
    10141 tatctgcaat tacctcatga aatgaatcca ttcttaggat atcgtgcatt gcgtatcagt
    10201 ttatctgaac aaggcgacga aatgttccgt acacaaatgc gtgcattgct tcgtgcatct
    10261 gttcatggta atttacgtat catgttccct atggtagcta cactcaaaga attccgcgca
    10321 gcaaaagcga tctttgaaga agaaaaacaa aaattgataa gtgaaggcaa ggaagtatct
    10381 gatacgatcc aagtcggcat catgatcgag attccagcag cagcagtctt agcagacaag
    10441 tttgcaaaag aagtagattt cttctctgta ggtacgaacg atttgattca atacacaatg
    10501 gcagcagacc gtatgaacga acgcgtttct tacttgtacc aaccatataa cccatcaatt
    10561 ttacgtttga tcaaaaatgt aattgatgca gcacacgctg aaggaaaatg ggcaggtatg
    10621 tgtggagaaa tggctggaga tcaaacagct gttccattgc tagtaggtat gggtctagat
    10681 gagttctcaa tgagtgcgac atctattctt aaaacgcgta gtttaatgaa acgcttggac
    10741 acagcgaaaa tggctgaact tgctgatcgt gcattgaaag aatgcgatat gatggaagaa
    10801 gtcgttgaac ttgttcatga atatgtaaaa taaacaacgt atcaaaagct ggtgtggctt
    10861 gtaaaagagc tacgccagtt tttttgttct tttttaagaa aacgtttttg tcagtaagaa
    10921 tagacaaaaa t
//
LOCUS       DACDSY010000097        10676 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDSY010000097 DACDSY010000000
VERSION     DACDSY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.098"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(594..1985)
                     /locus_tag="IWK44_002047"
     CDS             complement(594..1985)
                     /locus_tag="IWK44_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP9550545.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(2198..2584)
                     /locus_tag="IWK44_002048"
     CDS             complement(2198..2584)
                     /locus_tag="IWK44_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP9550546.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2640..3263)
                     /locus_tag="IWK44_002049"
     CDS             complement(2640..3263)
                     /locus_tag="IWK44_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP9550547.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(3327..3995)
                     /locus_tag="IWK44_002050"
     CDS             complement(3327..3995)
                     /locus_tag="IWK44_002050"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9550548.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(4007..4447)
                     /locus_tag="IWK44_002051"
     CDS             complement(4007..4447)
                     /locus_tag="IWK44_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550549.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4506..5178)
                     /locus_tag="IWK44_002052"
                     /pseudo
     CDS             complement(4506..5178)
                     /locus_tag="IWK44_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5363..5761)
                     /locus_tag="IWK44_002053"
     CDS             complement(5363..5761)
                     /locus_tag="IWK44_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP9550550.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5758..6531)
                     /locus_tag="IWK44_002054"
     CDS             complement(5758..6531)
                     /locus_tag="IWK44_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9550551.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6528..7211)
                     /locus_tag="IWK44_002055"
     CDS             complement(6528..7211)
                     /locus_tag="IWK44_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9550552.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(7378..8265)
                     /locus_tag="IWK44_002056"
     CDS             complement(7378..8265)
                     /locus_tag="IWK44_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9550553.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(8262..9131)
                     /locus_tag="IWK44_002057"
     CDS             complement(8262..9131)
                     /locus_tag="IWK44_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9550554.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(9268..10554)
                     /locus_tag="IWK44_002058"
     CDS             complement(9268..10554)
                     /locus_tag="IWK44_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9550555.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
BASE COUNT         3210 a         2312 c         1765 g         3389 t
ORIGIN      
        1 atctgttcta acattctcaa ctttgtcagt ttaccatagg aaaatgggaa aatctacgaa
       61 agctctcaac agaatcgtcc tacttaaata cgcttttctt taataataaa taagttggtt
      121 gtttttgtta tttacgcttt cacttttcgg aataaataat gtgtatttat atggaataaa
      181 taaaaaaaga ggtcgtgact aaaaacaagt cacaacctct ggtaaacggt gttctcctga
      241 caactcctcg gtcacaagac acaatattca acaaacatga gaagctattt tttgaaattg
      301 ctctttgtga ctcagagtga aacggcaggc tcacgacctc tttcttaaac ggtgttcaaa
      361 agcttgcatc tgcggtaata agctcaacca aacgtaaaat atgaggaact atttttgttt
      421 ggctgttctt attacttgat gcagggcagc tttttcacaa cctcttattc acggtgtttc
      481 agaagtagct tctcggaaat aagccgaaat ttcacaaaaa tttgagaaac tatttttagg
      541 aaacttcttc ttatttctta aagctgacca cttctatcac aacctctttt tgactatgca
      601 ttcacatcga tcactttaga atcatcattg atgacatcgc gatctaaatc tttgatgcgt
      661 gcacttgtca cgataccagc taccatgcta ccgctgacgt ttactgctgt acgtgccata
      721 tcgattaatg gttcaacaga gataactaac ccaacgattg ctactggcaa gttcattgcc
      781 cctaaaacga tcagtgaagc aaatgttgcg cccccaccga ctccagctac accaaaagaa
      841 ctgattgtta caattgccac aagcatcaaa ataaattcca aactaaagac attcactcca
      901 gctgctggtg cgacgatcgt tgccaacata gctggataaa cgccagcaca gccgttttgt
      961 ccaatagaca aaccaaaact tgctgagaaa ttagctgttg catcatctac tcctaaagct
     1021 tttgtctgag tctcgatatt caatggcaaa gctccagcac ttgaacgtga agtaaacgca
     1081 aagctcaata ccggaaaggc ttttttgaaa tagttgattg gattgacttt tgcaccaatc
     1141 aaaatcagtg agtggacaat caaaacagca agcaatgcta cgtaagaagc aacgacaaat
     1201 ttacctaaat tgacgatagc tgcaaaatca cttgttgcca aaacgtttgt catcaaagca
     1261 aatactccat aaggtgtcaa acgcaaaaca agcgtcacga tccgcataat gattttatac
     1321 agactgtcga ctaaatctga aaagaatgct gcttcttttg gcgcttttct tttcacgcct
     1381 agataagcga ttccgacaaa tgccgcgaaa ataacgactc caatcgtact tgttgcacga
     1441 ctacctgcta aatcagcaaa aacgttttta gggataaagg ataaaatctg ctgtggaatc
     1501 gataagtttt caactgtttc ctgacgtgtt gccagttcag agattcgtgc tgtttctgca
     1561 gctccttgtg tgaatttagc tccttggagt ccgaacagca ttgttgaacc aattccgatc
     1621 aatgctgcaa tagctgttgt tccaagtaaa gaagtcaata cagtcgcact aattttccct
     1681 agcttttcag aaactttcat ttttgtgaat gcaccaatga tcgaaataaa cactaaaggc
     1741 ataactagca tttgcaagaa tgatacatat ccttgaccta caatcccgat ccattccatt
     1801 gattgagtaa cgactttgtc gtctacacca aagatcaatt gaagcagacc gccaaagacg
     1861 ataccaactc ctaatgctgt aaagacgcgt gttgaaaatt ttgtatggcg cttttgcata
     1921 cgatagaaca catataataa tgccgcaaaa actagaacca ctaaaactgt gatgagtgtt
     1981 gtcatttttc tcctcctcat attattaatg acaagtccat cgaaggaagt aaaggttccc
     2041 gagaatgatt cttcaaccat cctcctgaaa aaaatttttt cagtgacttt tatattttag
     2101 acgattttta tcgaaatgac attctttttg cttacaatta gtaataaaaa aacatacctg
     2161 tgatagcttt aatctatcgc aggtatgttt ttactgtcta ttcttgtttt tctgattgaa
     2221 aattcaattt taccccaggc tgatctaagg cgatctctgt cacctcataa gacattcggc
     2281 gtacgatatc gaataacggt gctactagct cgtccacttc ttcttgtgtg acatcatttt
     2341 gaccttcaat tttccgattg aggatatgat tgatctgact cacacttcca gataaaacat
     2401 atgtatcaaa cactaaacgg aactctaagc gtgctcctac gatgctatgt tctttcggat
     2461 agttttcgat cgtttctttt aaaggcgaga agcctacttt caaatctgtt tctacttctt
     2521 ggttcggcaa tctcatatca taatgaaatg cttctacaac ttctttttgt cttctgattt
     2581 ccatataatt ttatcactcc tttatgaaaa ttataacata tctcctttga aaggggatac
     2641 tactttttaa tgatgctctg caaggtaagg atttcattca gatttcctac cacataatct
     2701 gccgggatct cacccagaaa atgatcgatg tcgtaaagac aagtagcgac tcctgcattt
     2761 ttccctgctt cgatatctaa acgacgatcg ccgatcatca tcgtgtctgt tctttctaaa
     2821 tgaaaagtat cgatcaaata atttaaagat gctggatcag gtttacgagg aaagtcctga
     2881 tcgatcccta ctacttcctc aatcagatgt gccaatcgat gttcttttaa caggccccat
     2941 gttgattctg tcaaacggtg ggtcaggatg aaatgacgac cgccgttgtc ttttagcatt
     3001 tccagtactt gtttcgtttc tggaaatgga cgggaaactt ccgtactttc tttttcatat
     3061 cggtgaaata attcattaaa ctctctggca ttcaagtttg attcattgat caaataacta
     3121 gtagaatact ttttcatttt gaagtagatt tcttttttgt ccatactgat accaaaatct
     3181 tttagtgctt gccatgcacc atctaccatt gcgggatacg tatcaaacaa tgtaccatca
     3241 aagtcccata tataattttt catgttcctt ttgttccatt cctttgcttc ttttttctat
     3301 ctctcacttt tagataaagg tgttctttaa agtatttttt tgtccagaat tgtcaaatca
     3361 tctgctaatg tatactggat tggttgtgca tttgggatat cgatcgtatc catttgatct
     3421 tcaggaacat cttctaaata ttttgtcagt gctcggatgc tattaccatg acccacaact
     3481 agtaaatctt gccgatcttt tagttttggt gctagatgat cttgccataa gggaatgatc
     3541 cgattcactg tcatttccag actttctccg tatgggatca aatgcggatc caagtgatca
     3601 tatctgcggt caaatctatt ttctgcggtt aacggcggaa cttccgtata tcctctgcgc
     3661 catgttttta cttgatcttt tccgtatatt tctttcatct tatctttgtt tttacccaca
     3721 agatccccat aatgccgttc atttagacgc catgttttta ttataggaag ccataactga
     3781 cccatttctt ctagtgcgac ttgtccggta agaatcgcac gtttcaaaac agaggtataa
     3841 acggcatcga attgaagtcc ggcttcaagc attttttgac cggcaactgc tgcttgttct
     3901 tttccttttt ctgttaaggc gacatctagc catcctgtcc agtaattttc aaaatttgct
     3961 tcgctttcgc catgacgtaa taatactaat ttcattcttt tctcccttaa acattcgttt
     4021 cgaattttat accacatgtt ttgcatgtca cacgtattcg tccttttcct ttaggtgcac
     4081 gctgcttttg atgacaattc gggcactcaa aaaaagtata actttttcga tccattttga
     4141 cttgcttttt gttttttatc tgatgatatt tttctttgac tctatcaaga tatttcaaat
     4201 aacgttgatt ctcatttagt ctggggtaga ttttttttga taggaaacga aatatcatcc
     4261 atccgaaaaa gacgatgaac aacaattgtc cgattctata gggcaaccaa cgacttagca
     4321 aactaaagac aagcgcagcg atcaatagac cgcgattcaa ctgatcaaat ttcgcatatc
     4381 ttccctgcat aagtttgtga tagttctgct gaaatcgtat tagcctttct aaccatgttt
     4441 gcatcatatt atctcctcca tcatctaata tttattatga caaaaaaatc caaaaagaaa
     4501 aagccttaat tggccttttc cattgcaact tggtgaagga cattccgatt tctggtaaag
     4561 tcaatcacaa tcactttctt tttatcttct ctgttgacta ctcgaaatgt gtccgtcaat
     4621 ggaagaaaca gcttcttaga tggtagctgt tgcatacgta taaaacgatg gatcatcgta
     4681 atcaattgag gaaattgaat agacgtgtga agcagacctg gatatttttt caacagctgt
     4741 gtcgttttga tcagattccg tttttttact gatcactttt aaaaagcttt ttagaatctt
     4801 ttgctgacgt ctaaaaatag aaaaagaatt ttcgtcatct atttgataac gcatttgttt
     4861 ttgcagttct gctgcagtca gatggatctt cccatcgttt gtatcgatca ttaaaccttc
     4921 tggaaacagt tcaaccactg ctcgatccca agattcaaga gaaagctcga tataatcttt
     4981 tactggtaat tccaattcta ccttaaaaaa gtgcaaaaca gccgacagcc cttctgcttg
     5041 atagatttct cctaatgtta gcccctctag atggaactct ggtgaaaaat gcagcaatgt
     5101 tttattttcc tgcaattgaa catatagaaa tgtagatcgc tcaatcagag tatcttttaa
     5161 aacaactaaa aaattcattt ttctccccct tttttcatca tttttaaaat agcatatttt
     5221 gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt cataagtatt
     5281 cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct tgtttgtgtt
     5341 tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta ttttcatcta
     5401 aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata ccaatgactt
     5461 tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg atgccttgac
     5521 caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac acaatatctt
     5581 tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca gaacctcttc
     5641 catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata acatcttcgc
     5701 ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca agatctttca
     5761 tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt catcttttcg
     5821 tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa tcattgcaac
     5881 cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga ttgccacgct
     5941 cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt tatcaaaagc
     6001 cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc cgtatcctgg
     6061 gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca atcctactgc
     6121 tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc tcccctcgct
     6181 caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc ctactaagaa
     6241 ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg caatagcact
     6301 gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga aaaggatcaa
     6361 cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt tgcgcatacg
     6421 aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg ccaaaatgac
     6481 ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca ttggaagatc
     6541 acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac cattccaaaa
     6601 ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc aagggccaag
     6661 ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc aatcggcata
     6721 aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat agccatcaat
     6781 tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa tactccagtg
     6841 ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga tatatcatat
     6901 acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat cccgatagca
     6961 aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt ccctactaga
     7021 tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat tccgatcatc
     7081 atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg tgcaaaaaca
     7141 aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa
     7201 ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt
     7261 cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc
     7321 aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta
     7381 ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc
     7441 agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga
     7501 agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac
     7561 taagggccat aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata
     7621 ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc
     7681 taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa
     7741 gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa
     7801 aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg
     7861 aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat
     7921 ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc
     7981 gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg
     8041 aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt
     8101 attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct
     8161 taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt
     8221 gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct
     8281 actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct
     8341 gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg
     8401 gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc
     8461 aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc
     8521 gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc
     8581 tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc
     8641 caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc
     8701 ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat
     8761 agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg
     8821 gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt
     8881 gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct
     8941 tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata
     9001 ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc
     9061 atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct
     9121 ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa
     9181 agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag
     9241 cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg
     9301 tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc
     9361 tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt
     9421 ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata
     9481 agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt
     9541 gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc
     9601 tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt
     9661 ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc
     9721 tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga
     9781 ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa
     9841 aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt
     9901 ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc
     9961 aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc
    10021 tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc
    10081 tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta
    10141 tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac
    10201 aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata
    10261 atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt
    10321 cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt
    10381 ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca
    10441 gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa
    10501 acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat
    10561 ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga
    10621 aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat aaaaaa
//
LOCUS       DACDSY010000098        10642 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDSY010000098 DACDSY010000000
VERSION     DACDSY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.099"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1232)
                     /locus_tag="IWK44_002059"
     CDS             complement(<1..1232)
                     /locus_tag="IWK44_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9550556.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSIS"
     gene            complement(1225..2124)
                     /locus_tag="IWK44_002060"
     CDS             complement(1225..2124)
                     /locus_tag="IWK44_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9550557.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2269..4047)
                     /gene="uvrC"
                     /locus_tag="IWK44_002061"
     CDS             complement(2269..4047)
                     /gene="uvrC"
                     /locus_tag="IWK44_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP9550558.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4229..4543)
                     /gene="trxA"
                     /locus_tag="IWK44_002062"
     CDS             complement(4229..4543)
                     /gene="trxA"
                     /locus_tag="IWK44_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP9550559.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4630..6990)
                     /locus_tag="IWK44_002063"
     CDS             complement(4630..6990)
                     /locus_tag="IWK44_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9550560.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7105..7653)
                     /locus_tag="IWK44_002064"
     CDS             complement(7105..7653)
                     /locus_tag="IWK44_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP9550561.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(7711..8148)
                     /gene="zapA"
                     /locus_tag="IWK44_002065"
     CDS             complement(7711..8148)
                     /gene="zapA"
                     /locus_tag="IWK44_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP9550562.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8350..9267
                     /locus_tag="IWK44_002066"
     CDS             8350..9267
                     /locus_tag="IWK44_002066"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP9550563.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9523..10434)
                     /locus_tag="IWK44_002067"
     CDS             complement(9523..10434)
                     /locus_tag="IWK44_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9550564.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
BASE COUNT         2998 a         2285 c         1796 g         3563 t
ORIGIN      
        1 gaaatcgatt gtttcaatga actccataat ccgccttcgc tatacacgag tacattcgat
       61 ttgtacatgt ttgcagacaa gatcatcaat aagaatgtta cggctacaag taccaacaag
      121 gaaatcaatg ctccacccat accgactgtc tcatttgcta agcgaaccgg cataatatat
      181 gaagataaga atggaatata cgaagtgatc cgaataacga tattgtttgg atctgaagct
      241 cctagtatca atccaagcat atatccgcct agagatagat aagtcactgg caaaattgct
      301 tttgctgtat cttctgcttt gttgaccaaa gagccacata aagctgctaa aacagaaaaa
      361 atcaaaatgc caaagatcat gaatagaaga gaataccaca agaatggtcc aatcaagtta
      421 tccaatgaga taccgtctaa tatatttttg accatttcca tatctttgaa ttgactgtat
      481 cctataccaa aaataatacc gtataatgcc atttgcgtta atgcaacaag taaaacacct
      541 gttagttttc cgtaaaaatg cttttgtgca gtcgtactgg acaggatcac ttccatgatc
      601 cgcgtgcctt tttcagaagc gatctcttgt gcaatgatct gcgcatacgt caaaataata
      661 atgaacaaga caatcgttgc tacataactt acgacatatt ggactgtcga gttatcttct
      721 ccaactgtca tttttccttt tgaatcaaaa ttgacttttt gtcgagtcaa acttgcttgt
      781 tggctcaagc tagctacttc ttctggtgaa atgcctaatt gacttgctcg aagcatcgat
      841 tgcaagccag tcagttgttg ttgtatcagt agttgtgtac tttgtcctaa tgaattttcg
      901 ctataaagca tcccatttat cttatcattc tcagttgaga ctactaaata cgcatcgatt
      961 ttttcttccg aaagctcttt ttgtgctttt tcttcagaat caactgcttt gaactcaaaa
     1021 tcatctgttt tgacttggct catttgctga ctgatttgct cattatcagc caatacgccg
     1081 attttattga catcactatt ttgttgagca aagtttcctg ctacataaat gatacccagc
     1141 atgataaaag ggactaatat catgatcaag aaagaaatcg acttaacatt ttttaaatag
     1201 acatctttgg taataatcca aaacttacgc atcgatttca cctgctttca atttgaaaat
     1261 ctcttccaaa gttggcggtt gctggttaaa cataggaatg tagccgaact gagtcgcacg
     1321 agtaaagatt tctttcccag cttcaggatc ttctaaggta atctctaacg tattgtcttc
     1381 atgaaggatc acttttttca cgccttcgat ttcttctact tcctgttggc ttaaaccaga
     1441 ttccaagaat aacttcgtac ggccaaatga ttcacgaatc tcatgtactt ttccattcaa
     1501 aaccattttt ccgtttcgca acatgatcaa atgatcgcag attttttcta cattatccat
     1561 attgtggcta gaaaaaatga cacatgaacc atgttttttc aattccaata ttccatcttt
     1621 tagcaattca gcatttaccg gatctaaccc gctgaatggc tcatccaaaa tgatcaattt
     1681 aggttcatgg attaacgtgg cgatcagctg gactttttgc tgatttcctt tagacaaaga
     1741 ttttacttta tccgtctttt tcccttttac ctgaaatttt tccatccaaa aatcgatttt
     1801 tggttcaatc tcttttcttg atttccctct aagttctgca aaataaagca gttggtcttg
     1861 aatcgttact ttcggataaa gtccccgttc ctccggaaga taaccgatga catcataatc
     1921 tttctcccca agatgatggc cattccataa cacttctcct tggtcttgtg tcaaaaaatc
     1981 caagatcaaa cgaaaagtcg ttgtcttccc agccccgttt tggccaatta gccccatgat
     2041 ttttccatcg gggatttgga aagagacatg atcgacagct gtgtagttcc caaaagtttt
     2101 aacaagatcc ttgacttcta acatttcttc actccttctt caagaaatca catatatcct
     2161 tagacaccag acgtaagcga tttattattt accaacaaaa cgtaaacgct ctttttgttg
     2221 tacataaaat gataggacac tttactttct ttgtaaagtg ccctttcctc attcctgatg
     2281 taatttatca aatacgtttt gtgcgacatt tttcggcagt ccgatttctt gtaattcttc
     2341 tgtcgtggct gctgtgatat ttttcaacga tttgaactct ttaagcagca ttttttttcg
     2401 ttttggtcct aacccttcaa tttcatctaa tcgggaagca aaactgtttt tactgcgtag
     2461 ctgccgatgg aaagtgattg cgaatcgatg gacttcatct tggattcgct ggagtaagaa
     2521 aaactctggt gaatttcgct caagcggtac tacagccaaa tctataccga atagcaactc
     2581 atttgtttta tgtttatcgt tttttgccat gccagcaata ggaatatcga ttcctaattg
     2641 attttctaag acatcttttg ccacatctac ttgtcctttt cctccatcga tcaagatcaa
     2701 atcaggtaaa ggcaaccctt cgcgaatcac tcgtgaatat ctgcgataga tgacttctct
     2761 cattgatgca taatcatccg gtccgttgac tgtttttatt ttatatttcc gatattcttt
     2821 tcttgccggt tttccatcaa tgaatacgac catcgcagcc acaggattcg tccccatgat
     2881 attagagtta tcaaaagact cgatacgtat tggggtcggg atattcatcg cgtttcctaa
     2941 acgttcaact gctccgatcg tccgttcctg ttttctggca atcagatcaa accgttcatt
     3001 caaagcaact gaggcattct tattcgccaa ttcaactaat tttttctttt ctcctcgttt
     3061 tggttgcagc actttcgtag ctaacaaagc ttctacactg ggcttgtcga tcgtatcagg
     3121 aatcaagact tctttaggaa tgaaatgctc attttcctga taaaactgac cgaggtaagt
     3181 caagaaatct tcttctgctt cgttatagaa aggaaacatt gaaacatctc gttcaattag
     3241 ctttccttgt cttacgaaaa acacttggac acacatccat cctttgtcaa tcgcataacc
     3301 aaatacatct cgatccagta aatcagtatt tgtcattttt tggcgagtca tgattgtctc
     3361 aatggcacgg atctggtctc ggtattctgc agctttttca aactccatgt tttcagcagc
     3421 tttttccatt ttctgatgga tctctgactc gattttttcg tgtcctccgt ttaaaaactt
     3481 tttcacttca tcaacgattt tcgtatagac tttcggatct acgtcgaaat aataaggaca
     3541 cagacattgt cccaaatgat agtaaagaca aggctccttc gtctgactag gtttgcattt
     3601 tctcagagga aataatcgat cgaggatctt ctttgtctca ttcgccgcac cgacatcggg
     3661 atatggtcca aaatacagtg ctttgtcttt tagtacttta cgagtaatca ccaaacgggg
     3721 atatttttca ttcgttatct taaggaaagg ataggtttta tcatccttta acataatgtt
     3781 gtatttagga tcatttttct ttatcagatt gatctctaag agcaatgctt cgatattcga
     3841 ttctgtaacg atgtattcaa agtcttcgat ttcactaaca agccgttccg ttttcgtatt
     3901 atggctgcca gtaaaatatg agcggacacg attttttaat attttggctt tgccgacata
     3961 aatgatcgtt ccatttttat ctttcatcaa gtaacagccg ggctgatcag gaagtaaagc
     4021 taatttgttt ttgattcgtt cgttcatctg gtactcccct tcatattcaa ttaaagttat
     4081 tatagcatgt ctgtcaaaaa aacaatccag cctgacaacg taattatact caaaaaatat
     4141 taatttgaat aaccttaact caaccgcaaa aaaaccgagg acggaactgg attcagtccc
     4201 accctcagct tatttttgct gtaagttttt ataaatattg atcgatcatt tgacgtagtt
     4261 gttcttttga atgaacacca acagcttttt ctactacttg accatctttt ttcaacatta
     4321 atgttggaat gctcatgata ccgaattgtt gaggagtttc tggattttca tccacatcca
     4381 ttttagcaat tctgaattct tcttcatcat attcttgttc caactgatcc aaaatcggtg
     4441 cttgcatacg acatggacca caccaagttg cccaaaaatc aatcaataca agtcctttat
     4501 ctgtttcttc attaaatgtc ttatctgtaa ttacttgtga catatcaaag acctccattt
     4561 ttaaaatcta tgattagtat agcatttcta tacttggata gcgaaccatc tgcttacctt
     4621 tagtaagctt tacttaaatt taacaattgt cgcaccattt ccgccttggt ttgccggtgc
     4681 aaactcaaag cttttgacgc tccgatggtt tttaagataa tcagtaattc ccttgcgaag
     4741 tgcgcctgtt ccttttccgt ggacaatagt cacttgagga taaccagcta gaatggcaga
     4801 atccaaatat tgatctacct cacttagcgc ttcttcgtat cgttttcctc gaaggtccaa
     4861 ttgattcggc acgtgactac tagattcaga tcgtacagca tggataactc gttgtgtcgg
     4921 ctctttttga ggagcaacag gcgtcagctc gtcttcggac acattcattt ttaatatacc
     4981 gatttctact tgccactgat tcccgttttt acgtaaaagc gtccctcttt ggccgtaagt
     5041 cgtaaccaac acttcgtctc ccggtttcaa cgttttttgt tctttcgcct tcttcaatac
     5101 tttatttttc ttcaaagttt cttcttgatg tagatttgct aattgggtct ttgcatcgat
     5161 caactgatgc tctttcacat ttccttgccc aatctgctgt tgcattttac gaatatcagc
     5221 aatgattttt tctgcttttt cttccgcctc tgacacgact tcgtttgctt ttttctttgc
     5281 tttttccatt tctttttcac gttcttcaaa gaaatagctg taagcttctt tcaaatcatc
     5341 gtgaagttct tgtgcctcag aaacaaaatg acgcatttcc agatattcgg tctctgccat
     5401 tttccggcga ttctctaaat ctgtgatcat ttcgtttaaa tcttggctct catcattcat
     5461 caactgcttc gcttcatcaa taacttctgt atcaagacct aatcggctag agatttcaaa
     5521 cgcattgctt cgtccaggaa cgccaatcag caaacgatac gttggactta gtgtatccac
     5581 atcgaactcc atgctggcat taatcgtatt tgcccgattg tatccataaa ctttcaattc
     5641 aggataatgc gttgttgcca tcacataagc agacttttta ccaagatcat ctaagattgc
     5701 gattgctaga gcagcacctt cttgcggatc tgttcctgca ccgagctcgt caaataatac
     5761 cagacttttt tcattgactt ttgataaaat atctacagtg ttcgtcatat gagaagaaaa
     5821 ggtcgataga ctttgctcaa tcgactgttc gtctcctata tccgcaaata cttcctcaaa
     5881 aataccgatt tgactttctt cacctgcagg aatcggtaat cctgcttgcc ccatcagttg
     5941 taacagacct aaagtcttca acgtgatcgt tttcccacct gtgttaggtc ctgtaatgac
     6001 gatggcttga tagtcttttc cgattgtgat gtcattagaa actacttttt cctgatcgat
     6061 cagtggatgt cttgcttgtt tcaagacgac atgattgtct tcactgattc ccggcacgat
     6121 ggcttttact tctttaccaa atcgtgcttt tgcattcatc agatccattt taccaatcac
     6181 ataagcattg tggatgattt cttgacgatg gggaacaagt tcagcagaaa gttcacttaa
     6241 aatacgttga atctcatttc gttcagcgat ttggtattga cgcaaacgat tattcaaatc
     6301 caccacttgt tttggttcaa taaataaagt ctgtcctgaa gcactttgat cgtggactac
     6361 accgccaaat acaccgcggt attcttgttt gactgggata acgtagcggt catttcgcat
     6421 ggtgatgatc gcatcactta aatactttgc atttttccca cggacgatcc catctaactg
     6481 ttcacgaaca gcttgttcac ttcgccgaat gttctgtctt atgctcttta actcaggaga
     6541 agcgtcatca gtcacgcgtc cgtcttcatc gattgcttcc tttaaacggc ggctcaaaac
     6601 agggatcgtg actaactgat ctgcccacat atacaaacgt gcaaattcga tttcactgtc
     6661 agaaagatca tcaatgaaac gattcaattc gcttgtcgta gacaatactc tggaaacttg
     6721 tgcaagctcc actccattca agtctgctcc gatctcgatc cgtttcatat ggggacggat
     6781 attttcaagt ttaggtatag ggatcccgcc tcgcagacgt tgcactttca atccatcctc
     6841 agtttcttga agccagtttg tgatcgtttg tttctcactc actggtacca attctgccag
     6901 ttcttctttt ccttgtgcag tcactacaaa ttgtctgacc atctgtttga ctttttcaaa
     6961 ttccaatgtt tcaaggattc ggttattcat tttatcacct tatctttcac tatgaaaaaa
     7021 gagctgtgcc ggacatacgc tacgcagcct tcttaaataa actactctgt acaaaaaacg
     7081 aaatattttc cagcactatt ttccttaatt caaaatattc gttacccaaa gctcatacat
     7141 cttgttggta agaaatggcg ttctctcaac gataaaacga gcgaaactat gctcgccgaa
     7201 ttgattttgg accacatcta atggaacaaa ggcaagtaac tgcaaaatca aaaatatagt
     7261 aacgtaagcc atgattagtg acaggatgcc tcctgcaatc cagtctacct gtctcaagat
     7321 cggcacatac gtcaatccat ggacaaaaac ccccacaaat cgggtaagca gccagccaat
     7381 tagcaaaata atcagaaaag cagttcctgc ataaaaagct tcatctaggt gaaaagcaaa
     7441 tacctgatca aaaaatgcta actttgatgt tggggtgaca gctgggtatg gaatgtataa
     7501 ttccaaatga cttgctaatg gtttgtaaaa atgctgggca gcaataaatg acaatacata
     7561 tccaatcgcg aatatgattt gcaaagcaaa cccgcgacgt gctccagaaa aaaacgcaat
     7621 tagcaataaa aataaaatca gcaaactcag catgctacgt tcctttctct tctaactttt
     7681 ttaaattttt cttattttga taaaaggatc ttaattagaa gatttttgtt taatttgtcc
     7741 ttttcgtttc tcgtttaaaa tctgttgtgc ttccacatga tttttaatgg tatgatctgc
     7801 ttgaccattt tctttcaata cttcccgagc ttcgttctcg attgcttcaa tgcgtttcac
     7861 acgattttca agctctgtaa atttgatcat ttttttcttt aattctgctg tttcttcttc
     7921 cagctccaag attctttctt gtttcttcaa ttgatccgaa agcgcattga cagccatcaa
     7981 gattgctgct tgttcattat ccatttgtgg tgataattgt ttcaattctg ctaactgttc
     8041 attgatcagc ttcgttacga tatccatatg atgcttggtt tcccgtccga taatagtata
     8101 tgtttggttg gcaatcactg ctttgtagcg tgttttttcg tgcgccatac gaatcatttc
     8161 ctcctatttg atgaaagttt tatttagtgt accatgaagt tggttctttc accgttctcc
     8221 acaaatattt ttcacatact taactattat atgataaact ttaaaaaaat gctatgtctc
     8281 gcaatctctt agaaaacgaa aagtcgtgag ttgcggtata gttttatgta taaaaacgag
     8341 gagaatttta tgagcaacgt cattatcaaa gcaacaaacg accaacttag tcaaatgaaa
     8401 gcatactatc gctcttattt gttaaataaa cagattcctt atactagctt tgctgcaaaa
     8461 aaagacggga cgaccattac cgcttataca tccggaaaag tgatgtttca aggtacgaat
     8521 gcagcaatag aagctgataa atggggggag gcacaggata aacaagccaa gccagcaaaa
     8581 acagcctctt ctttgccaaa aaatatcagt caattatccg ttttaggtag tgatgaggtc
     8641 ggaaatggca gctattttgg tccgctaaca gtatgtgctg cttatgtgaa acgtgatcag
     8701 cttgcagaat tacgtaaact tggtgttaga gattcaaaag agttaaaaga tagccaaatc
     8761 atccagcttg ctgacatctt aaaaaaaacc atcccctata aactgcttgt cttatctcca
     8821 gaaaaataca atgagatcca accaaaatac aatgccgttc ggatgaaagt cgctttgcac
     8881 aatcaagcga tccatttgct gctaaaaaaa atcgagccag aaaaaccaga agcgattttg
     8941 atcgatcagt ttacgccgga agcaaatttt aaaaaatatg cgcgtttaga aaaaaatcaa
     9001 ttacaagaaa aactgtactt cgttactaaa ggagaacaat atcatttagc tgttgctgct
     9061 gcttctatca tcagtcgtgc cagctttttg gaagagttac acaaagcatc agctgaagct
     9121 ggaatcaccc ttccatcagg agctggaacg aaatctgacc aagtagcggc aaaactattg
     9181 gagaaaggcg gcatgcctat gcttgaaaaa tatgccaaac ttcattttgc caatacagaa
     9241 aaagctttga agctgctaaa aaaataatgg aactgtgaca aaagtcatgt cacagtccct
     9301 tgttccgaat aaacggtgtt ctcatgccaa ctcctcggtc acaagtcaca atagtcatca
     9361 aacatgagaa gctgtttttt gaaattgctc cttgtgacgc ggagttaaac ggcaggctca
     9421 caacctcttc cttaaacggt gttcaaaagc ttgcatctgc gataataagc ccggccaaac
     9481 gaaaaatatg aggagctatt tttgtttggc tgggcttatt tattactgaa aataagtggt
     9541 caatgcttgt gtcaaacttg cagcaatctg ctgctgataa ctgtctgtta ctaattcctt
     9601 taaatcacta tcattattca tataacccat ctcgattaaa atagaaggtt gaagattttc
     9661 tcgaagaact tgatagttcc cagtggagat tcctcttgaa tccagaggca gatcagtgaa
     9721 ggagtcagca attgtctgtg ctaatgtttc ctcttgtcca tagtaataat atgtcgtgat
     9781 tccagttgct tcattcgcat attccgtaga gtctgcgtgt aaactgataa agacatctgc
     9841 ctttttttct tcactgtagt cgcatatatc tccaagctgt actaattcat ctgtagtatg
     9901 ggtcaaataa accgttgctc cagcatcctc tagcgccttt tttactttta tggctgtact
     9961 taatgttata tctttttctt ctgtttgttc atcattgctt aatgccccag tatcatttcc
    10021 gccatgccca ggatctaata caatcactgc atcagatagc tcttccggtt ttgtttgtat
    10081 attctttgtg actttggttc catcttcgag ggctaaatat ccagtatagc catcatttgt
    10141 cttgatctga taagcatttt ttgtttcaga aagaaactcc gccagctctc cgccgtttat
    10201 agttgcaagt ggggtagtta gcgagtcatc tgtatatagt gtacggtcat tttttccgat
    10261 tttgattaat gtctcttgtt ctgtatcatc aaatgattcc tttgtctttg taaccgtact
    10321 gttcgtactg ctgaccgttt gtccaactat ctttttttga ccaaaaacat ctatttgaaa
    10381 acagataata atgataatga ttgcaaatac aaaagctcca attatttttt tcattcttga
    10441 ctctcacctc gtagatccag actaagggtc aaaacttaaa atcagcttaa gtacgcaaaa
    10501 aaggggctgt aaaagatata accgtagaaa ataacattac tgctaaaatg atttttttca
    10561 tttttggcat atcctgaaat ctccttttga ctattcgtgg tgattcatct acccaataaa
    10621 tagtttcctt cattctacaa ac
//
LOCUS       DACDSY010000099        10578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDSY010000099 DACDSY010000000
VERSION     DACDSY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.100"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(25..144)
                     /locus_tag="IWK44_002068"
     CDS             complement(25..144)
                     /locus_tag="IWK44_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9550565.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IWK44_002069"
     CDS             complement(159..1343)
                     /locus_tag="IWK44_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9550566.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="IWK44_002070"
     CDS             complement(1599..1853)
                     /locus_tag="IWK44_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550567.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="IWK44_002071"
     CDS             complement(1878..3020)
                     /locus_tag="IWK44_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP9550568.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="IWK44_002072"
     CDS             complement(3080..4432)
                     /locus_tag="IWK44_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9550569.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="IWK44_002073"
     CDS             complement(4503..4847)
                     /locus_tag="IWK44_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9550570.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="IWK44_002074"
     CDS             complement(4857..5231)
                     /locus_tag="IWK44_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9550571.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="IWK44_002075"
     CDS             complement(5244..5558)
                     /locus_tag="IWK44_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9550572.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IWK44_002076"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IWK44_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="IWK44_002077"
     CDS             complement(8976..9638)
                     /locus_tag="IWK44_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550573.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="IWK44_002078"
     CDS             10255..10485
                     /locus_tag="IWK44_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550574.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT         2915 a         2347 c         1794 g         3522 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaag
//
LOCUS       DACDSY010000100        10515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDSY010000100 DACDSY010000000
VERSION     DACDSY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.101"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(219..704)
                     /locus_tag="IWK44_002079"
     CDS             complement(219..704)
                     /locus_tag="IWK44_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP9550575.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1184..1825
                     /locus_tag="IWK44_002080"
     CDS             1184..1825
                     /locus_tag="IWK44_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9550576.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1844..2479
                     /locus_tag="IWK44_002081"
     CDS             1844..2479
                     /locus_tag="IWK44_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9550577.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2497..3312
                     /locus_tag="IWK44_002082"
     CDS             2497..3312
                     /locus_tag="IWK44_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9550578.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3631..5067
                     /locus_tag="IWK44_002083"
     CDS             3631..5067
                     /locus_tag="IWK44_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9550579.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5256..7703
                     /locus_tag="IWK44_002084"
     CDS             5256..7703
                     /locus_tag="IWK44_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9550580.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7933..9000
                     /gene="rlmN"
                     /locus_tag="IWK44_002085"
     CDS             7933..9000
                     /gene="rlmN"
                     /locus_tag="IWK44_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9550581.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9193..10296)
                     /locus_tag="IWK44_002086"
     CDS             complement(9193..10296)
                     /locus_tag="IWK44_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9550582.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3472 a         1799 c         2284 g         2960 t
ORIGIN      
        1 cgatttataa tcgaaaaaag aagcattttt atatatgctt cttttttcgt agaatcaata
       61 aatatggatt tggctaacta tggaagaata ctcgtattag ttttcagcgt tcatactagt
      121 tgttacattg tttgattggc caaaagaatt tctgatatat tttgtgctaa ataaagaaaa
      181 agacgcttga attgggaaat aagaaaacta ctctatcatt acaaattttc ataaactggc
      241 agatgttcta agtaaacaag tatcttttca aagtcggaaa aatccataat caacgtagtg
      301 gtattgatat ttggatgaaa gcctacggta tcttgacgat caatagcagc atcgatcacg
      361 acttggattt ttttctcttt gtcgtgcatc aatgctaatg gagtcactgt acctgctgga
      421 acgcctagga ggtcaggaag ctgttcttca gaaacaaaag ataagcgctt ttcttttaat
      481 tgttcagcta gttgttttaa atcggcgtgc ttttcatcag gcaagatgac aagatagatt
      541 tgttttcctt ttcttgattt caataataga ttttttactt gcggaccagg tagggaaaaa
      601 gggacatttt tgactgacgt aatcgcagga tgatcgactt tttgaaaaga gatacccagt
      661 ttttttagta gttcataagc ttcttgttcg gttgcacgac tcatcgcgca tcaccctcct
      721 ttttttatta gtgtatcata aaaaagagtt gacaaatgaa aaataatcat gtaaattaca
      781 cgtaaagtta aaacaaatga catcgaaaag aagagtagcc gttaggtgtt tttagagagt
      841 ctctggttgg tggaaagagg cagcagttaa ccgtgaaaca catcttggag tcgattttct
      901 gaagcgtata gtaggaaaat ccggggagcc cgttacagct gcggtcgttg ctagatgacc
      961 agataaggct ggcttttccg tgaggagtcg gtaaaaaaag gtggaaccac gtgattaacg
     1021 tcctttgatc ttcggatcaa aggacttttt gttttcatct gaacggcaac gactgtgtga
     1081 ggttttttct gcaaggaaaa aataaacaga ggtggaacca cgtataatac gtcctcttgg
     1141 catttgccaa gaggattttt ttattttaaa ggaggaaaag aagatggagt atttgattga
     1201 agttatgccg caattattag taggagcact cacaacattg aaattatttt tatttacctt
     1261 gattggctca ttaccattag ggattgtttt agcattagga ttgtctttgc attttaagcc
     1321 gctacgctac ttgcttcaaa catatgtatg ggttatgcgg ggaacaccgc tgttgttgca
     1381 actgattttt attttttatg ggttgccgac gattgggatc gtttttgatc gtttcgaagc
     1441 agcactcatt gcctttatct taaactatgc agcttatttt gcagagatct ttcgtggagg
     1501 gatacaatcg attcctcaag gtcaatatga agcagcgcaa gttttgcaat taagtccctt
     1561 tcaaacggtg acgaaaatcg tcatcccaca agtgacaaag attgttttac cttctgtagg
     1621 gaatgaagtt atcaacttgg tcaaagattc ttccttgatc tatatattag gtttaggtga
     1681 cgtaatgcgt gctgggaaaa tcgcgatgga acgcgacgcg actttactgc ctttgctagg
     1741 cgtggcaatc atctatctta cattgactgg tattctaaca atcataatga aacaagttga
     1801 aggaaaatta aattattacc gctaactgat aggaggaaga aatatgattg aattgaagaa
     1861 agtgaacaaa gcatttggtg aaaaaaaaat aatcaaagat ttgaatctaa tcattccaga
     1921 tgggaaaaca ttagctatcg taggtccttc cggtggcggt aaaaccacgc ttttacgtat
     1981 cttggctggc ttagaaagtt ctgacagcgg agaattttat ttagacggtg taccgttcaa
     2041 cccattcgag cttaaaatga aagaacaagt tgtaggagtc gtattccaag acttccagct
     2101 atttccacat ctgaatattt ttgaaaacat taccttagca ccaaaaatgg tattgaaaca
     2161 gacaaaagaa gaatacacca aaaaagcaga agaattagcg gcttcattag ggattagtga
     2221 tttattgaat atctatcctt atcagctttc tggcggacaa aaacagcggg tagcgattgc
     2281 cagagcattg gctatgaatc cacgggtatt ggcttatgat gaaccaacaa gtgctttaga
     2341 tcctgaatta cgccagcaag tcgagcgttt gatcctatca ttgaaagaag atggggtaac
     2401 ccaaattatt gtcacccatg atttagtatt tgcagaaaat gtcggtgatc aattactaaa
     2461 agtttcccca attcattaag aaaggagaag atagagatga aaaaaaccag ttttctattc
     2521 gctttgatcg caggtcttct tctttttgca ggctgttcga acaaaaaaac ttcagcagat
     2581 aattgggcga ctatcaacga agagaaaaaa atcgttatag gtttagacga tacatttgtt
     2641 cctatgggat ttagagataa agacgggaag ctcacaggat ttgatattga tttagccaaa
     2701 gcagtctttg aacaatatgg gattacggta gactttcagc ccattgactg gtcaatgaag
     2761 gaatttgaac tgaacaatgg aacgatcgat ttgatatgga acggctattc caaaacgccg
     2821 gcaagagaaa agaaagtgca atttacaaaa ccttatatgg aaaacgatca agtgctgatc
     2881 acacctaaag aatcaggaat cacaaacttt ggacaaatga ataataagag attaggtgcg
     2941 caaaatgggt catctggtta tgatgtattt acgaaacaac cagaagtatt aaaagagata
     3001 gtcaaaaacc aagatgctgt tttgtatgat agttttaatg aagcattgat cgatctgaaa
     3061 agcagacggg tcgatggtct gctgatggat aaagtatacg cagactatta tttgaaacaa
     3121 agaaacgaac tagaagactt caacataaca aaaggttctt atgagagtga ggattttgca
     3181 gttggtgcac gcaagtcaga tagtacatta gtgaaaaaaa tcaatgaagc cctagatcaa
     3241 ctagagaaaa caggtgaatt ccaaaaaata tccgataaat ggtttggcga agatgttacg
     3301 ccgagaaatt aacaaatagg ggactgtgac aaaagacgtg tcacagtccc tttttccgaa
     3361 taaacagtgt tctcctgcca actccttgtg actcggagct gaatggcagg ctcacaacct
     3421 cttggtaaac ggtgttcaaa agccagtttt tcggtattaa gccaaattta tgcaaaaatg
     3481 tagaaagcca ttttcacaaa atttttctta ctactcgaaa ctaagcgact ttttcacaac
     3541 ctcctttaca taattttatt ttgttgtgga aggggtttcg tgttattcta aaagaggaac
     3601 gattgaaggt tataggaaag gaagagatga atggaacaca aagaacttac ctcatttcca
     3661 aaagattttc tgtggggctc tgcttctgca gcgtatcaag tagaaggagc ttggcaagaa
     3721 gatgggaaag gagaatcagt ctgggatcga tttgtacgga tacctggaaa gacatttaaa
     3781 ggaacgaacg gtgatttggc agtagaccac tatcatcgtt ataaagaaga tattgcttta
     3841 atgaaagaac aaggtctgaa agcttaccgc ttctctgtag cttggacacg gatttttcct
     3901 aatggacgag gagaagtcaa tcaagctggg ctcgcttttt atgagagatt gatcgacgaa
     3961 ttgattgaaa acgatattga acctatgttg actttatatc attgggatct tccacaagca
     4021 ttgcaagatg aatataatgg ttgggaatca cgtcagatca ttgaagattt cactcattat
     4081 gcagaaacat tatttgaggc attccgcggg aaagtccatt actggattag tttgaatgaa
     4141 caaaatatct tcacttcatt aggttatttg ttagcagcac atccgccagg tgtgacagac
     4201 ccgaaacgaa tgtatgaagt taaccatatt gctaatttag caaatgcatc tgtcatcaat
     4261 aaatttcacg agttagaaat gcctggaaaa atcggaccaa gctttgcgta tacgccaaat
     4321 tatccaattg acagtaatcc tgaaaatgtt ctggctgctg aaaatgcaga agacctgatg
     4381 gccaattatt ggatggatgt ctatatgtgg ggaaaatatc cgattgctgc catgaggttc
     4441 ttagaagaaa aaggatgggc accaacgatt gaagctggcg atgcagaatt gctagaatct
     4501 gctaaaccag attttcttgg gatcaattac tatcaaacgg cgacgaatgc attcaatcca
     4561 ttaaacggag ttggtgctgg aaaaatgaat acgacaggaa aaaaaggttc atcagaagaa
     4621 actggagttc ctggaatgta taaaaaagtt gagaatccat ttgtcgaacg aacaaattgg
     4681 gactgggaga ttgacccaga aggattaagg atcggactgc gtcgtatcac aagccgttac
     4741 cgcatgccgg tactgattac ggaaaatggc ttaggagagt atgacaagtt gacagaagat
     4801 aaacaaatcc atgacgacta tcgaatcaat tacttacaaa gccacgtcaa agccatcaaa
     4861 gaagcaattt cggatggagc agaagtccta ggctattgca cctggtctta tacagatttg
     4921 cttagctggc tgaacggtta tcaaaagcga tatggatttg tatatgttga tcaagacgaa
     4981 acacaagaag gatctttgga acgatataag aaagacagtt attactggta ccaaaaactg
     5041 attgaagaaa atggagagaa cctttagttc acatacttaa aacaagaagg tacggctgga
     5101 tatggccgtg ccttttcttg ataaaatcat ttagaaatta agaaaaactg taaaataagc
     5161 tgaaaaatga aagaaagcgt cagttctttc aaacaaaact ttagttccct tacttttttt
     5221 tgtataatga aacttgatga aatgagggat aataaatggc acaaaagaaa cggcctacta
     5281 aaaaaaggaa aaagacgaaa aaaacgaaaa agcaaaaaca gcagcaggaa caaatgctga
     5341 cgattggtgt tgggtttgcc ttgattttat ttgctgtctt cggcttttta aaattaggtt
     5401 ttttaggaat attgattgca aacggctttc ggatcattgc aggaaatact taccaaatcc
     5461 tctgtctttt attagccgta ttaggatttt ggattgtcat taagaataca gagttttcaa
     5521 ttggtaaaaa ccgacgctgg tttggcggca tactctttta tttcggtatc ttattattgt
     5581 tacatgccca tttatttggc aaactccata ctggggaacc aaatatcatg gggacgacat
     5641 gggatttgtt agccagtgat ataaaacaaa gtcaagtaga caataacgtt ggtggtggga
     5701 tgattggagc aatcctttat cattttactt acttcttgat tgcacagcct ggaagttatt
     5761 tagcagcagt gctattactg gcaggaggag cattcctgtt cagtaatctg gaaggctatc
     5821 agctgttaaa tggtatccaa tcaattggag aacgagtaca ggaattattg gaaggtgatc
     5881 ctgccaagca agctcgaaag caagcggcaa aggaagagag aatgaaacag cgcgcagaag
     5941 cgaaagaagc aaggcgctta gctgcaaaag aagctgcaga aaaagaagct gcagaatatg
     6001 agaagaaaaa agcggtgagc cagcaaaggg aaaatactgc tgatgaatgg caggaaccag
     6061 aaaatacaga gccggagcag ctaagttttg ttccaatcga cagttttcaa gaaaatatcc
     6121 atcctgcgaa tctagagaaa ccagtaccag atacaccaaa acagaccaat acagcagaag
     6181 gtttttcaga tgagttgcca gaggatgacg gtacctcatt agaatttgag attgaagcag
     6241 aacaggaaaa tcaagactat gaattaccga cagttgattt gcttgattct ataccaactg
     6301 tggatcaaag tgatgagtat aaaaaaattg aaaaaaatat cggcgtatta gaacaaactt
     6361 tccaaagttt cggtgtcgat gccaaggtcg tcaaagccag cttaggacca tccgtcacaa
     6421 aattcgaagt ccagccggct gtaggggtaa aagtaagtaa aattgtgaac ttaacagatg
     6481 atatcgcgct tgctttagcg gcgaaagatg tccggatgga agcaccgatt ccaggtaaat
     6541 cactgattgg tattgaagta ccaaatggaa agatcagtat ggtttccttt agagaaatca
     6601 tcgaagcaca accaaaccac ccagataaat tactagaagt acctttagga cgtgatgttt
     6661 ctggacgggt acaaacggct gatctttcga agatgcctca cttactcgtg gctggttcga
     6721 ctggtagtgg gaagtcagta gcaattaatg ggattatcac aagtatatta atgcgtgcta
     6781 agccccatga agtaaaactg atgatgatcg atccgaaaat ggttgaattg aatatgtaca
     6841 acggcatacc tcatctgttg acgccggtcg tgacgaaccc tagaaaagca gcgcaagcct
     6901 tacaaaaagt agtacaagaa atggaagaac gatatgaaaa attcgcggca accggagtaa
     6961 gaaatatttc tggttacaat gaatttgttc aacagaaaaa cttggaaaac ggaacgaaac
     7021 atccaacctt accattcatc gtagtgatcg tagatgaatt agctgatttg atgatggtag
     7081 ccagcaatga agttgaagat gcgattattc gtttagcgca aatggctcgg gcagcgggga
     7141 ttcatatgat cctagctact caacgtccaa gtgtggatgt tatcacagga atcatcaaag
     7201 caaatgtacc ttccagaatg gcatttgcgg tatcaagcgg tacagattct cggacgatca
     7261 ttgattctaa tggggcagaa aagctcttag ggcgaggaga tatgctattc ttgccaatgg
     7321 gtgagaataa accgatccgt gtgcaaggag cattcatttc tgaccacgaa gtagaacggg
     7381 tcgttcaatt tgttacagat cagcaagaag ctcattatga agagaaaatg atgccgacag
     7441 atgaagttga aacgacaggt gctccagaac agccacaaga tgagctgttt gaagaagcaa
     7501 aagcacttgt cgttgaaatg caaacggcaa gtatttccct tctccaaaga agatttcgga
     7561 tcggttacaa ccgagcagca cgtttagtag atgaactgga agctcatgga gttgttggcc
     7621 cgtcagaagg aagcaagcct cggaaagtat tcattgagca ggaagaaaca tcagaaacgt
     7681 tgccggacga taatgacatg taaaacttgt gtgatgaagg gttgaaaaac gaataaccaa
     7741 taaaaagaca aggcatgatg ataccaaaag aaaaaatggt ttttttccat cttttctaat
     7801 acggtatgat gtcttgtctt tttagttttt gtgagattaa agttctattc gatttatttg
     7861 tgaagtattg cattaaggaa atgaatcatg tatagtatgt agcgaaactt tgagaaaaag
     7921 agggaaaata ctttggaaaa acagtccatt tatggcttga cgaatgaaga attaatcaat
     7981 tggtttatag aaaacggcga gaaaaaattc cgagctgccc aagtttggga atggttgtat
     8041 caaaaacgtg taagtaattt cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa
     8101 ttatcggagc attttatcat caatcctcta aaacagatgg tcgtccaaga agcttcggat
     8161 ggtacagtaa aatatttgtt tgaactccct gataaaaaca tgattgagac tgtactgatg
     8221 agacaagaat atggcttgtc agtttgtgtg acgacacaag taggatgtaa tatcggctgt
     8281 acgttttgtg caagcgggct gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt
     8341 gcacagatca tgatggtcca acattatttt gatgagcgca atcttggcga acgcgtttca
     8401 catgtcgttg taatggggat cggtgagcca tttgataatt acgacaatgt aatggacttt
     8461 ttgcatatca tcaatgatgc caaaggattg gcaattggtg ctcggcacat tacagtttcc
     8521 actagtggat tagcacacaa aatcaaggaa ttcgctaata acggcttgca agtcaatctg
     8581 gctatttcct tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg gatcaaccga
     8641 agtttcccaa ttgagaaatt gatggaagct gtggatgagt atttagagaa aacaaaccgt
     8701 cgaatcacgt ttgaatatat catgttgaat caagtaaatg atagaccaga gcatgcgcaa
     8761 caattagcag atctattaaa agataagaaa aaattagcat atgtcaactt gattccatat
     8821 aatccagtca gtgaacatga tcaatattct cgcagtccaa aagctgatgt attgagattt
     8881 tatgatgtat tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat
     8941 attgatgcag cttgcggaca attgcgaagc aagcaaatga aaaaaacagt tgccaaataa
     9001 tcgtatccta ctcgtttttt caattaccat aaaaggagca gttttcttaa ttaaggaact
     9061 gttcaactta aataagagaa aagattctat agcgaaagct ataaagcttt ttttgacaag
     9121 aaaaaccctc atcaagcaaa gaaatgtcct caatttgatg acagcctgct tgatgggggt
     9181 tttagcaatt atttaactga ttttcttata tccttgctgg ctttcttgtg tagcatgata
     9241 agtttctacg tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat aaggcacatg
     9301 tttaatatca tttgttgcca tggctttatc taagattgct tctaaatcgg tcgtgacatt
     9361 gagattcatg tcggttaacg atactggaag ttggttcttt ttcataaaca catagatttt
     9421 atcaaacaaa tcgaactgct gatccatcat caataaggtc agtacaccat aactgacaga
     9481 tgctccgtga aggtatccct ccattttttc tagaacagtc gtaccgtaat agaatgagtg
     9541 tgccatagca gaattatagt tattgttaac taaaacagaa accaatccgg tagtaacaat
     9601 gatatccgaa acgacttgtt caattgcata tgagggacga tcgtcggcac tatctttcaa
     9661 tgcttgcgta ccataagtca agagacgatc aatgcacatt tttccgattt ggattcctaa
     9721 tctatccgtg tagcctagat catcgccacg agcagaaaaa gtagattcga cttctttgga
     9781 taaagcatcg ccaatacctg cccataagta tttatcaggt gcttcggcaa taattgctgt
     9841 atcaatgaag gtgtgtacag gtggttttga aggaaagaag agtccgtcca ttacgccttc
     9901 ttcgttatag atgacacata ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa
     9961 ggtgaagact ggtttagcta gtttttcacc aatgactttt acagtatcgc aagcacggcc
    10021 accaccgact gcaaacagga cgtctgcttt ttggacactt tcatgctgga tcaactgttc
    10081 aatatttcca taagttgaat tacccccgta ccagagactg gcgacaacct ctatttcctg
    10141 tgtcaaagct acttttattt tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag
    10201 cactactttt gtcccatatt ctttgacgat tccatttgct ttttcatacg catctgttcc
    10261 aatggtataa tttggtaaga aaacactttg actcatattc atttccttct ttcttcgttg
    10321 aaacgggtta aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt
    10381 gaacctaaga caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa
    10441 gtatcctaaa aacgaacaag tacaaaaaca ttgaaaacga caataatgaa tttttgttcc
    10501 tcttagtatt tctac
//
LOCUS       DACDSY010000101        10236 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDSY010000101 DACDSY010000000
VERSION     DACDSY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.102"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            169..318
                     /locus_tag="IWK44_002087"
     CDS             169..318
                     /locus_tag="IWK44_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP9550583.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            329..1843
                     /gene="dltA"
                     /locus_tag="IWK44_002088"
     CDS             329..1843
                     /gene="dltA"
                     /locus_tag="IWK44_002088"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP9550584.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1840..3045
                     /gene="dltB"
                     /locus_tag="IWK44_002089"
     CDS             1840..3045
                     /gene="dltB"
                     /locus_tag="IWK44_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP9550585.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3090..3323
                     /gene="dltC"
                     /locus_tag="IWK44_002090"
     CDS             3090..3323
                     /gene="dltC"
                     /locus_tag="IWK44_002090"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP9550586.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3326..4594
                     /gene="dltD"
                     /locus_tag="IWK44_002091"
     CDS             3326..4594
                     /gene="dltD"
                     /locus_tag="IWK44_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP9550587.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4735..5817
                     /locus_tag="IWK44_002092"
     CDS             4735..5817
                     /locus_tag="IWK44_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP9550588.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5817..6620
                     /locus_tag="IWK44_002093"
     CDS             5817..6620
                     /locus_tag="IWK44_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9550589.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6690..7430
                     /locus_tag="IWK44_002094"
     CDS             6690..7430
                     /locus_tag="IWK44_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9550590.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7441..8445
                     /locus_tag="IWK44_002095"
     CDS             7441..8445
                     /locus_tag="IWK44_002095"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP9550591.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8490..10187)
                     /locus_tag="IWK44_002096"
     CDS             complement(8490..10187)
                     /locus_tag="IWK44_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550592.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3287 a         1694 c         2080 g         3175 t
ORIGIN      
        1 aaaagtacga gaaattataa agaaaaaagc agtgatttga tgtgctattt ttataaaagc
       61 tatgtgacaa aactgttagt aaaaaaacgg tttctgtaag ctagacacat gtttcttttt
      121 gtcaaaatta gttacacttt gtgtgaggtt atatgaggag ggaatccaat gaacaaatgg
      181 cgttctaatc caaaattgaa ttattgggga aaattcatca ttcgaacaat tttttatagt
      241 gcagtactgc tgttcctgat ttatctgtat cactataaaa atattcaagg aggaactttt
      301 atctataacg aattttagga ggaagagtat ggaaatcaaa acgattattg aagcaatcga
      361 tgattgggga ataaatgaac ctgatcgagt tgcttatcag gcggatgaga cgcatacttt
      421 tggtgaattg aaacaagcat cagacgcact tgcttattat ttagaaaaaa aggtagaagg
      481 cgatggacca attgtcgttt ttggcaattt ggagtttgaa atgattgtct catttctagg
      541 ggttgtcaaa tctggccatg cctatttgcc gattgaagag catacaccca aggagcgtat
      601 cctatcgatc tttcgggtgg ctaaaccatc tatggtcatc agtatcgggg actggataga
      661 agatattcct tcggttcctg tcataacaaa aaaagaattt caagaaatca agaagatacc
      721 agtaggattt tatcccagaa atagcgtaaa aggtacagaa aatttttata ttatttttac
      781 ttctggtact actggagaac caaaaggcgt acaaatcagt catgataatc tagtcagctt
      841 tgttcaatgg acgatggaag actttgggat caagcctggt atgcatttct tagcccaagc
      901 gccattttct tttgatttat cggtattttc tatttatcct gcattagttt ctggcggaat
      961 gctgaagccg ttaaataaag cagttgttca agactttcgt caattattcg cgacattgcc
     1021 agccttgaag ttgaatgtgt gggtttctac tccatcattt atggatattt gcttgatgga
     1081 acctacattc aatggagaaa atgtgccgga attggacatg tttttatttt gcggagaaga
     1141 attgacgaaa aaaactgcag aaagtctact tgatcgattc ccaaatgcac ggatatataa
     1201 tacttacggt ccaacagaag caactgtagc tatttcaagt attcagattg accagcatgt
     1261 actagatagt tatgaccgac taccgattgg ctacgtaaaa aaagacacga aagtttctat
     1321 tgtccaagaa ggtaaacctg tttcaaaagg agaaacgggt gaaatcatta ttgcaggacc
     1381 aagcgtatca aaaggttacc tgaacaatcc cgaaaagaca gctgctgcgt tcttttgtca
     1441 aaatgactta gcaagttacc atacagggga tgcaggtaga ctagatgaat atgggctttt
     1501 attttatgaa ggacgtatgg atcagcaggt caaattacat ggataccgga ttgaactcgg
     1561 ggatatcgaa cattatctgc tccaagacaa ccgaatcaaa caagctgtcg tcgtaccaaa
     1621 ataccaagga acaaaagtac agcagctggt tgcttttgtc gtattagaaa acaaaacgaa
     1681 acagccggat tttcagttga ctaaatctat caaagaacaa ttactagaag ttgtaatgga
     1741 ttatatgatt cctcagaagt tccattatgt cgatctgctg cctcagacag ttaatggaaa
     1801 aatcgatcga aaaaaattaa cagctgaggt gaatcctgaa tgatgttacc tcatatgatc
     1861 ccttatgcag agccgtttta tttttttctt ttagccattg cgcttgtacc gatcgttcta
     1921 tctttattga tatgggaaaa aagattacca gtttatcaat cagcagtgac acttttcttt
     1981 ttatttgtca gctttggagg taattattgg caacagggag cggcgctgat tgggtatgtt
     2041 ttgtggcaga cgatacttgt atggggctat tttgtttatc ggcaaaggaa aaatcagaca
     2101 ggatggtttt ttgccgccgt tattttagcg ataattccac tgtttttaac gaaagtgact
     2161 cctttttttg aacatggaaa agcatcgtta cttggttttt taggaatctc ttatttaacc
     2221 tttaaatctg tacaaatgat catggaaata agagatggca tgatcaaaac atataagcca
     2281 tttcgttata tccagtttct gctgttcttt ccaacaatct cttctggacc aatcgatcgc
     2341 tataggagat ttgaaaaaga tcagctccat ccgcctgttc gtgataaata tgttgatctt
     2401 ttgggaaaag gcgtccaaaa tatttttgtc ggttttttat ataaattcat tatcggttat
     2461 tattttggtc aagtactgct tcctgtagtg ggacgcattg ccttacagca tggtggtatt
     2521 tcatgggcat tagtaggtta tatgtacgtg tacagtatgt atttgttttt tgattttgct
     2581 ggctatagct tatttgcagt cggtacaagc tacatgatgg gatatcagac accaatcaat
     2641 ttcaataaac cttttttatc atggaatatc aaagaattct ggaatcgttg gcatatgacg
     2701 ctgtcctttt ggttccgtga ttatgtatat atgcgattga tgttcttcat gatgaagaaa
     2761 aaatttttta aaagcaagat cgtaatgtca aatatcggtt attttgcttt gtttcttcta
     2821 atgggtatat ggcatggatt gacgtggtac tacatcgtat atggactgta tcatgcttgt
     2881 ctgatctgtc tgacagatgc atggatccga tttaagaaga agcataaaga gcagttgcct
     2941 tctaacaaat ttacgcatgg cttagccgta tttttgacat ttcaagcagt atgttttagc
     3001 ttcctgattt tttcaggatt tttagataaa ctttttttta gctaacgaat agtgacatat
     3061 taatttatat aacaaaggag aaaaggaaaa tggaagaaca aattttatca atcttagaag
     3121 aaataacagg aacagatgaa gtaagaaaaa atcgtgatat tgatctattt gatgaagggt
     3181 tgcttgattc tttagcatct gttcaattat tagtcgagct agacggagag ctggatatcc
     3241 aagtccctgt atctgaattc gagcgagagg attggagcac accgaataaa atcattcaac
     3301 aagcaactgc attgaagggt tgagtatgaa aaagaaactt tttgctattt tcggcccggt
     3361 tgttttagct gttggcttac tgtttgtgtt ttttacctcc tcctatcaag tagatatctc
     3421 taatccagaa acgattagac gtgcctcttc ttccatgtca gtgaacgtgt tgaaaggaga
     3481 tgcgataaaa aattatgcat tatctgaaaa acaatatatt cctttttttg gctcaagtga
     3541 attaagcaga atcagtccat ttcatccgtc tgttctggca gaaaaatacc agcgaaacta
     3601 tcgtccattt ttgctgggag cacccgggac acaatcgtta agccaatata tgatgatgcg
     3661 ttctgcagga gatgctatga aaaacaagaa agtcgtcttt attatttctc ctcagtggtt
     3721 cgttaaaaat ggtgtaaaaa ctgattactt taatacgtat tattctgaac ttcaaacata
     3781 cgactggttg ttttcaatga agaaagtaac gcctgctgat cgctatttag ctagaagatt
     3841 attaactttc tcaaaagtaa aagagaatga tacgttgacg gcgattctgc aaacgatcaa
     3901 aaaagggaag ctacctttac ctgaatcttt gaatcagctg cgcagccaat ggaacatgct
     3961 gaagagagag gatgaagtat tcagcaatat tgggttgaaa gatcgacagc aaaaaatcga
     4021 tcatgagtca aaacgattac caaaacaata ccaagaaaca gaattatcca ttttagcaaa
     4081 tcaaattgga gaacgagaaa cgacgaataa tccattcggg ttgaaaaacg atttttatac
     4141 gcatcgtatc cgagcccacg agccagagct caaacagtca caaaaaaatt gggattatcg
     4201 tttttcgcca gaattttctg attttcagct ggtcttggat caattagcaa aaaaccataa
     4261 cgaagtattg ttcatcatcc caccggttaa cgagaaatgg agcgattata ccggactatc
     4321 tcaagaaatg ttacaaggat ttgccaaaaa gatcaaattt caattaaata gccaaggatt
     4381 caatcgaata gctgattttg tcaatcaagc gggtaccaac tattttatgg aagacacgat
     4441 ccatctaggc tggaaaggct ggttggctgc agatcaacag atcagaccgt ttttagaaga
     4501 aaatcatatt actgctagta agtatcatct tgacgatgct ttttttagca aatcttggca
     4561 acatcaaata ccagataaac tacaattaaa ataaagaaaa caggcgagtg ttgctcgctt
     4621 gttttcttta tttttgaata ttctgaataa aagataaaaa taccaagttt gcctgaacaa
     4681 aaatcataag caatggtata atcacaatat gaatatagaa atgggggaaa acatttggat
     4741 acaaaagaag aacagctact tattgattta acttctgcaa aaggagtacc tggaaatgaa
     4801 gaagaggtaa gagaagtatt cagagaatat gccaaaccat tcgctgatga tattttttat
     4861 gacggtttag gcagtgtgat tgccaaacat ggtgctggcg gaggccccaa ggtctttatt
     4921 tctggacata tggacgaagt tggttttatg gtaacaaaaa taaccgaaaa aggattcttg
     4981 gaattccaaa ctcttggagg ttggtggggg caagtcatgc ttgctcagca agttgaaatc
     5041 aaaacacgtg aagggaaaat cgtccacggt gtgattggtt ccaaaccacc acatgtttta
     5101 acacctcaag taagaaataa accttatgaa ataaaggata tgttcattga tattggcgca
     5161 tcaagccaag aagaagccaa agagtggggc atacgtcctg gagatatggt cactccatat
     5221 attgaataca aacgaatgaa tggcagtaaa tatttactgg caaaagcatg ggataatcgt
     5281 atcgggacag ctgtttctct gcgagtatta gagaatctat ctaaagaagc acacccaaat
     5341 gttttattcg caggaagcga tgtgcaagaa gaagtaggct tgcgaggtgc tcgaacaagt
     5401 acacaccttg taaatcccga tattgctttt gctttagata ctggtacagc aggagacact
     5461 ccaggaatga caccaaaaga agcagattcg attttaggaa aaggcccaca gattttgatt
     5521 tttgatgcat ctatgatccc gcataaaaaa ttattgaatt ttgttattga tgtagcagag
     5581 gaaatggata ttccattcca atatacagtg atttctggcg gaggaactga tgcaggacaa
     5641 atgcatttga caagagatgg tgttccgtct gtagccatta ccgttcctgt tcgttatctg
     5701 cattcccata catcagtgat tcatgaagat gattatttga atacagtgaa actagtcaca
     5761 gaattagtga aacggcttga tagtgaaaca gttgaaaaaa ttcggagtta tgaataatga
     5821 ataatagaat acctatttct gaatatcgga aaaaagcacg agtttctttg gaaaatcaat
     5881 ggggaatcaa tacttggctt gttttcttat ctataatatt tacgagcgtc atccagaact
     5941 tttttgacgg tttcagtcct tttccggaag aaagcatgca gcagcactta ttgagttttc
     6001 tagtcgataa tttactttta ttcgcattca actatgcatt gttttacatt gcgctagtag
     6061 ttgtacgggg tggcagacca aaaagtaacc ttttattttc agttttccaa agaaagtatt
     6121 ttggttcgat acttggcatc aatttgatca attcagtcat caactggcta ctcaacagtt
     6181 taatctttct gcccatgttt ttaatcagcg gagtgagtac ttatatgcag ctgcttttca
     6241 gttcaaatgc ggtacctacg ggtgtcatgc cagaagattt tcttgatatc agctttgttt
     6301 tatcgatgat agggatgatt tttgtttcag cactcatcat gagtattgtt ggcggtttat
     6361 tccaatttgc tgcatggaca aagttcgact atcctgattt gtcggttatt caatgtttga
     6421 aatatggctg gtttttattg aaagatagaa ttggtacgta tattttgttg cagctgtctt
     6481 ttatcggctg gtatttgcta ggagcaatcg ctttgttcat cggtcttttg tgggtcattg
     6541 tttatgtgaa tgtgtcagtt gcagaatttt atgaacaagc acggattgaa aaaggaggtc
     6601 cagacgccta ttttacttag catgtaagca gtttattcat caaaaaaata acttttgtgg
     6661 taggataaat ctttgagggg gaatgtacga tgaaattaac tgttttagga tgcttaggag
     6721 cgtatcctta taaaaatcaa gggacaacag gttatttgct tcaatctgac gggttcaatt
     6781 tgttgatcga tgcaggtagt gctacgctga tcaaattaca agaacacctt gatccacttg
     6841 atctagatgc tgtgatcctt agccattatc atcatgatca tattgctgac cttggtgtat
     6901 tacagtatta ctggcaactt catcctaaga gaaatccgga gacattgctt tcaatttatg
     6961 gacacacaaa agattccatt cactttgaag aattgacgtt ggaaaatgtc tcaaaaggac
     7021 atccttattt tgaagctgaa gaattaaaaa ttggtccttt tctgattact tttatggaaa
     7081 caatccatcc agtagtatgt tacgccatgc gtatcgtaga agaaacaacg gggaaagtat
     7141 tcgtatttac aggtgattca ggttatttgg aaagtttcgc tgattttgcg aaagatgctg
     7201 atttgtttct agcagatacc tatctttttg aaggaaatga acatcatcat gcccatttca
     7261 cttcaaaaga agctggagaa cttgcgaaat cagctaacgt aaagaaactt gtacttacac
     7321 acctacctca gtttggtgat ttagaacaat tgaaaaatga agcgcaaaag gcagcaggca
     7381 aagggattga tatagaactt gctgcagttg acaaagtgtt taacatttag gaggaagaac
     7441 atgatttttg taccaaatga aaaccacgac ccaagaatca atttagcaat tgaacaatat
     7501 ttgctgcaag aaatgccttt agatgaacct attttgctct tttatatcaa tgaaccttca
     7561 atcatcatcg gcagaaacca aaatacgctt gaagaaatca atcgagatta tgtggaagaa
     7621 aatggcattc atgttgtccg tcgcttaagt ggaggcggag ctgtctacca tgattttgga
     7681 aacctgaact ttagttttat catgcctgat gatggggatt cattccgtga ctttgcgaaa
     7741 gtaacacagc caatcatcca agcacttcat gaattgggtg ttgctggggc ggaattgaaa
     7801 ggccgaaatg atctagtcat tgatggcatg aaattttctg gcaacgcgat gtatgctacc
     7861 aacggtcgga tgtttgctca tggaacaatt atgttcgact ctgatatcaa tgaagtagtc
     7921 aatgcattaa aagtaaaaaa agacaaaatc gaatcaaaag gaatcaaatc gatccgttct
     7981 cgtgtaacga atatcaaacc atttcttcca gaagaaaagc aaaaaatgac aacagaagaa
     8041 tttcgtcaag aaatcttgtt gaaaattttc ggcgtagagt cagtagaaga agtaaaaacg
     8101 tacgaattga ctgatgaaga ttggaaaaaa atcaatcgaa tctctgagga atattatcga
     8161 aattgggatt ggaattacgg gaagtctcca gatttcaatt tctctcgcca aaaacgtttt
     8221 tctatcggtt cgattgaagt ccatttgaat gtctcagaag gaatgatcaa agaagcaaaa
     8281 atctttggcg atttcttcgg actaggagat atctctgacg tggaaaaaaa attggttgga
     8341 cagaaatatg ataaagcatc tctatcaaaa atagtcagcc aaattgatgt gaagaaatat
     8401 tttggtgcga tagaaccaga agaattgctt accttacttt attgaaaata aaaaatagcg
     8461 gaatccacat atatggattc cgctattttt tatacaggat tcgggtaatt tcgagagagt
     8521 tgaacagtga tccgatcaat gaaagtatct ttttcctgcg ttagtgttcc tgtgtaacga
     8581 tccgctacgt ctttcatcat cattaagtca aaacgttcct gatcatctaa ttcttctgtg
     8641 atgcctcgac taaataggtt tgtatcgtaa tgtgcactat gttcgcaaca aatgacgaaa
     8701 tgttcctctt gttcgtgtat cgacaaataa atttttcgat acagtggatt ttcgatggca
     8761 tgagtttctc taaaggaatg ctctaaagcg tggatcaaaa gttgagccag atcgtcatcg
     8821 ttgataggaa gttcttcggg aagatctgct tggaaggtta tctgaatatt ccgtttggca
     8881 gctaacttct ggtaacgcgc taagataaga tttgtcacct ggtggttgca aaaagaacac
     8941 agcatttcga aattttttgc ttcttctaat atcttttcca catagttttt agcttttgtt
     9001 tggtccgtat gcattaaatc ttgcagattc tccatctctt ggacaaattc caggcgaagt
     9061 gtatttactt cttggatatg ctgtcgaagc tgctcataat gttgctccaa caaatcattt
     9121 ttgattttta agaaatctac ttgtctccga tctttgtcgg tatttaagat gtgatcgatt
     9181 agattgtagc tgataataac gattagaatg ataatatata aaatcgtaga ccaattgatc
     9241 gttgtttgat agatacctaa tgccgtttgg atgtaaatca agcaatggat aatggctacc
     9301 aagacgatcc aaggtgtaca agctacataa aaacggttat tcttatatgc ttcgccaatg
     9361 catgcaagtg aagtgactaa agtgcttata acactagcaa tattagcaat cggcattaat
     9421 tcaggaagtt ctgcaggagt aaaagttgca taaagcagaa cgccgataaa aatcaaaagt
     9481 tggaacgtac aaaaaattcc gtaccatttt ttatagtaaa acattcggct ccaatagtag
     9541 cagatgatga aagctgacgt taagataata aataaatgag ataaaaaaga atgaacgaat
     9601 ggttcaaaaa gaataccact taaaatatct tctgatacag cttcaaatcc taatgccaaa
     9661 gcaaagcaac ttaagagaat aagtgagtat tcaagacgtt tttgtttata catcaagtag
     9721 atcgtataga aaaaggtccc tatagcgata aaaacagcga ttatcatcgt caatacttgc
     9781 ggcactgatt gtgaataaat aaaactaatc aatggtccag ttgttcctag ataaactttc
     9841 ggtggcagtc cagcatagtt ctgataagga gatgagactg taagggtcaa tttttttccg
     9901 atataatttg ctggtaaatc tatcacagcc aatgagagtc cgggattgct ttcttcctga
     9961 taatctttcc gttggtataa aatcttttcg cctagatata cagtaatcca ttgatggttt
    10021 cctttgatta gtaaatttgc ttcgtccaac ggtttttcaa gtgtcgtttc cataatgaat
    10081 gtttcgtctt tatccacggt aggaatcgta cgagtgtact tgctgtcaaa cttaaaatca
    10141 ggatcatctt ttgtatagaa aacccacgaa tcttttattt ctttcatttg attgctgtcc
    10201 gcaggagaaa aaataaaata aaagaaataa gcaatt
//
LOCUS       DACDSY010000102        10200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDSY010000102 DACDSY010000000
VERSION     DACDSY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.103"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(361..873)
                     /locus_tag="IWK44_002097"
     CDS             complement(361..873)
                     /locus_tag="IWK44_002097"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP9550593.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(887..3184)
                     /gene="recJ"
                     /locus_tag="IWK44_002098"
     CDS             complement(887..3184)
                     /gene="recJ"
                     /locus_tag="IWK44_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP9550594.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3326..3760)
                     /locus_tag="IWK44_002099"
     CDS             complement(3326..3760)
                     /locus_tag="IWK44_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP9550595.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3770..4558)
                     /locus_tag="IWK44_002100"
     CDS             complement(3770..4558)
                     /locus_tag="IWK44_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9550596.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4555..5496)
                     /locus_tag="IWK44_002101"
     CDS             complement(4555..5496)
                     /locus_tag="IWK44_002101"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP9550597.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5673..6260)
                     /locus_tag="IWK44_002102"
     CDS             complement(5673..6260)
                     /locus_tag="IWK44_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP9550598.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6481..7794)
                     /gene="obgE"
                     /locus_tag="IWK44_002103"
     CDS             complement(6481..7794)
                     /gene="obgE"
                     /locus_tag="IWK44_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP9550599.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8082..9092
                     /gene="gap"
                     /locus_tag="IWK44_002104"
     CDS             8082..9092
                     /gene="gap"
                     /locus_tag="IWK44_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9550600.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9177..10112)
                     /locus_tag="IWK44_002105"
     CDS             complement(9177..10112)
                     /locus_tag="IWK44_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9550601.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2808 a         2330 c         1769 g         3293 t
ORIGIN      
        1 aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc ataccgaacg
       61 gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt aaacttttac
      121 gggggtgtga caaaaatcgg tcacatcctt tttttcgaat aaacggtgtt ctaaagccag
      181 tttttcggta ttaagtcaaa ttgcggaaaa agtgattttt taaacttcta ctggtctttg
      241 cttgtttcca tttataaaaa aatagcgacc ctcaaaagtc agatagagaa tctgactttt
      301 gagggtcgct ttaaaatcgc ttctttaaga ataacatcaa gaatagcttc ttttttctgc
      361 ttaatattcc attaatgtaa tgatgtcata tcctttgatt ttgtcgcgtc catggagatc
      421 catcaattcg atcaaaaatg cgcagcctac gactacaccg cctaattgtt cgatcagttc
      481 gatcgtcgct ttgattgttc ctccagtagc tagcaaatcg tcacaaatca gcacacgttg
      541 acctggttta atggcatcct tatgcaatgt caatgtatct gttccatatt ctaatccata
      601 agtcacttcg atggtttctc gaggtaactt cccttttttg cggactgggg caaaaccgac
      661 tcctaactca taagcaacag gacatcctac gatgaatcca cgggcttctg gcccaactac
      721 catatcaatt cgtttttctt ttgcataatt tacgatttgt ttagtagctt cccgataagc
      781 gtcaccatct gccatcagtg gggaaatatc tcgaaacaca attccttttg acggatagtc
      841 gggaatactg gcaatataat cttttaaatc cactctttgt ttcctcctag gttgttaacc
      901 atttctttat ggtaggaata tcgctcaaaa gcaaaaattc ctcaactttg attttattga
      961 gccgtcgctg gtataatctg ctctcagtca aaggatgact ttctgggcta gcgacttttt
     1021 gtaaaacacc gttctctatt gtaacaaatt tcaattcaaa aaacacctgt atcataaaaa
     1081 ttaataattt ttcagggata cgcaaatatt gagctattgc ttttatttta tgacgtatat
     1141 caattcgttc ttgagaatgg atcaatttaa acaattttgc gtactgttct cttgtgccta
     1201 ccccattcaa ataggcctca tcaggtgaaa tccccatcag ataaacacga ttgaatgaat
     1261 aagaccgaaa aacttctttc agatccgcta aatcatctgg acaatccata acaactagag
     1321 agtttggttt tcttttttca attattgcct ttacagaatc tagtccttcg aaaacaatcg
     1381 cagcttcctg gataatctca ggcacgaatt tcaacgtcga agaatcaaat gctaaataaa
     1441 ggctaccttc cgacgttcgg ctctgttcac ggaaacgttt tgcccgccaa tcaaatactt
     1501 gcagaccacc cacagaaaaa tcagatacca tcagctgcgg tttcttacgc ccgttccatt
     1561 cattgatcga aagctttcct accacatcta cctgattatt cagcagttct tcttcttgag
     1621 taccaaaacc aaaagcgacc gcatcaagct gactcgatgc atcggaaagg acaaatttca
     1681 gatgctgttg gtttgctccg attcttttga tattttctgc ttggacttgt cgaaataaaa
     1741 actgaggcaa aggattatcc gtaccaaatg gcgccaatag tttcagctca tcaattcgct
     1801 ctactgttac ttcatttggc agcaatactt catcaattct caactcaggc cctctcatca
     1861 agtcaatttg atgatcgact atatattggt tcatcttctc ctgtagtata gtgacatttt
     1921 cacttggcat cgttaaaccg actgctgcat gatgtcctcc gaagtaagta aataaatctc
     1981 gcatctggtc aagcatttca aaaagattca aggcttcaat actacgtcca gatcctttag
     2041 ctgatccatc ttcttttaaa gtaaggacaa gcgttggttt acccgtttca ttcattattt
     2101 tcccagcaac aatgcccaaa acgccttcat gccatcctcg gtttaccaat agatggatat
     2161 gattctcctt attgaccatc gctaatgctt cttgtgttat ctgctctacg attgcttttc
     2221 tttcttcatt gatctcattc aatttttttg cttgaacgga tgcttcctct tcatcaaacg
     2281 tcgcaagtaa cgagacggca ggacgtggat cgcccatccg accgattgca tttagtctgg
     2341 gagcaatcga aaaaccgatc gttgtttcgt ctgcgtccct catttttacg ccgctttctt
     2401 caaatagcgc ctgaagtcct atccgttctg aatgatgaat ggcatcgatt cctaatgaaa
     2461 caaggattcg attctcatca gtcaatgaaa ctaaatcggc gatcgttcct atcgctacta
     2521 aatccaaaaa ttctgcaggc ggttcttcca ataatgcaga agctactttg aatgctactc
     2581 cgactcctgc caaatctgga aatggatagt gccccgctgg atgacgaggg tgaacaatcg
     2641 cataagcgtc tggtaaattc tcaggtagtt cgtgatgatc tgtaatgatc acgtctacac
     2701 cagcttgctg agcgtatgct accgcatcat ttcctgctac cccgttatca acagtgatga
     2761 tcaactggat tccttcttcg atcttttctt ggtataccga ttggtttggt ccatacccat
     2821 gctcaaatcg atttggcagg aatacttcta cttctgcacc aagtaattcc agtgtttctt
     2881 tcattaccgt tgcactggtg atcccatctg cgtcataatc accgtaaacg aggatttttt
     2941 ctccactgat tacagcttct tggattcggc tgattgcttt atccatatcg tgtatcagat
     3001 acggatcatg taattcttgt tcctttggat gtaaaaatcg gttgatatcc ttttcctcat
     3061 ggtaacctct ttgccacagt aacttaccga caaatggaga taatttcagt tcacggatca
     3121 tttggagaaa ggattcagaa ggctctgtct cgcttaactt ccaatcaaaa tttgcttgtc
     3181 tcactttctc actcctaact tagttattat agcaaaggaa atcagcaaaa aaaagcagaa
     3241 agaaagaata aaaacagatc agatccgcta gacagatgtt cagcaggacc tgatcttttg
     3301 tacgaccatt tatgaaatat ccctattagt caaaataatt gaagctttgt ttgggcgatt
     3361 gagttttttg ttcttccggc gctttcaatt gactgatttg tgcggttttc gcttgtaact
     3421 ccgcctgata agcagcttcc aactccgcac gttcgtttct aaactcgcgt tgctgttctt
     3481 cttttgcttc ttcaattttc ttttgaagtt ctgtttgata ttctgacatt tcctgagtca
     3541 gctgtttgat ttgctttttt tgctgccata gtgttgccga agcagtcaaa aatacaatca
     3601 gtgcaccaat cagcgcagaa ccgatgatca ctaagatcaa tggcgcagaa aaggaagtga
     3661 agccaaaact tacaggaaca tttttattgt tcaatacagc aaaaagaaca ataatcaaaa
     3721 ctaaaacaaa tccaagaatc acattgcttt gctttttcaa ttccttcacc tactttttgt
     3781 taaaaattcc gctggctaaa taatcaccaa tcgttggaaa cagcgtataa aattttgatg
     3841 caacttctaa aataaacggt tgattgattt cccgttttgg atgtctcata ttgcggacga
     3901 tcatttttgc caacttggca ggttctaaaa cgatttgtcc gactttttcc aaatagcttc
     3961 cgcttggatc agccttatcg aagaaatttg tctcgattgg acctggattc actgtcgtaa
     4021 cagctacgcc aagtggtttc aattctaatc gcaaagcgtt ggagaagcca aggactgcaa
     4081 atttcgtggc agaataaacc gttgattttg cagttgccat ttttccagcc atagaggcga
     4141 tattaatgat atgaccttgt cttctttctg ccatatcgat cgcaaatttt tgtgtcaaaa
     4201 ccatcattcc taagatgttg acttcaaaca tatcgtatgc ttttccaagg tcgaatgtaa
     4261 ggaagtcttc gaaaatccca aaacccgcat tgtttaccag aatatcgacg cgcccgactt
     4321 cttccatcac cttttcatat aattcgtcca cgctttcagg attgctgacg tctaccttga
     4381 aagcataagc ctctttgccg ctcaagcgtc tgcactcgtc tctgacacct tctacaaatg
     4441 ccattcttcg tgcgcaactt ataacgattg ctccttgctt tgctgcttca taacagattt
     4501 gtgcgcccag accgcctgaa ctacctgtta ctaaaacaac tttatcttct aaattcatgc
     4561 ctcttcctcc tcgcgaaatg gaatctcaat gctatcaaaa tctttcataa tcgtcgtgtt
     4621 ttcaaacact tcttgtgctt ctttttcaag ctctaaagct gcttttccta aatatctcgc
     4681 actgatatga gtaagtagca gccgttttgc ctgcgcttct tttgcaactt cagcagcttg
     4741 atgagtcgtg gaatggaaat agttgtgcgc cattcttgct tcatctttat taaatgtgct
     4801 ttcatgtacc agaatatcgc tattttccgc aagcacaacg gaattatgtg tttttctcgt
     4861 atcgcctaaa atagtcacga ttcgtccttt tttatctgga cctacgaaat cttttccatt
     4921 gatcgtccgc ccatcttcta gttggatcgt ttcaccttgc ttcagtttgc cgtacagtgg
     4981 tccagctgga atacctaatt cttttagacg atccacctgc aattctcctt tatgatcatg
     5041 ttcaaccaca cgataaccaa aactgtcgat cccatgattt agtttttttg tataaacaga
     5101 aaattgctga tcagtaaaaa tcggggctgt ttcatttagc tcaatgaatt tcagcggata
     5161 acttaaacgc gtttgcgaaa tacctaaaga gacttttatg tattcctcta tcccttttgg
     5221 cccgtaaatc tctaatggcg tatccccacc ttgaaatgac cggctgctga tgagcccggg
     5281 aagtccaaaa atatgatcgc cgtgtaaatg tgtaatgaaa attttaccga tttttctcgg
     5341 acgaatagtc gttcgcaaaa tctgcatttg cgtgccttcg ccacagtcga acaaccaaac
     5401 ttcattgcgc tcgtctaaaa gtttcaatgc tatacttgtc acatttctat gttttgcagg
     5461 aacacctgca cctgttccta aaaattgtaa ttccataatc tacctgactt tctttaagtt
     5521 cctctttaat tttagggtaa aaagaaggga taatcaaccg taagtcgaga atgggttcaa
     5581 attaaaaata acgttctaaa gcgatagcgt tttctttgaa aattactata aaaacagcag
     5641 cgaacccgtt tgagatcctc tgctgttttt tattaatttg aagatccaga actttcattg
     5701 tcgttggaag tttgcggatg tttgtccttc caagtctttt gtgcagcttc tttcgcatga
     5761 ttttcagctt ctttcgcatc atgcacatct ttttcgacat cttcataatc caagcgagta
     5821 atttctccgc ctaattccat cattaccaat tgattcagtg ggcgattgtc ctcttcatca
     5881 gcaatcaaga tcagtcctgt agctccttca ccgattttat ttcctacaaa gtcgaacacg
     5941 gtctgggcat cttttatttc tttggcatct tgtgtcgcac cgatcattcc tccggcaaac
     6001 catcctaata aaatccctag cggaccacct aatacaccaa ccaacattcc gattaatcca
     6061 cccgtagaag atttgttatt accggtaaag tcgataaaat cttctatttt gaattgatgt
     6121 gtcccattag gatcatggct gacaactgcc atctgttctc ctttaatcga acgaacagca
     6181 tgtaactttt tgatttctga aaatgcctga tatgcctggc tttctacttg aaaatttaaa
     6241 atgattactc gtttgctcat taaaaacgcc cctttcatac cttcttgtta tttattttac
     6301 ctttaattga aaaataagaa aagaaaagca ctcttttttt ccaataaaat agggctgtga
     6361 caaaagtcgt atcacagctc ctttttccga ataaacggcg ttcttgaagc tgatcacttc
     6421 tgtcacaacc tcccttaaat ctgaatctat attttttggt atttgttttt atttgcttgc
     6481 ctattcgaca aattcaaatt caaactcacc gatacgtaca aggtcgccat ctttcgcacc
     6541 gcgtgcccga agagcttcat ccacacccat accacgtaat tgacgagcaa aacgcatcac
     6601 tgtttcatca tgatcgaaat tcgtcatttg gaataatttt tcgatcttct caccagataa
     6661 gatccacgtt gcatctgaat cacgatcgat ttggaactca ggaccttctg gattgaatcc
     6721 atagtgtaca gtttcttctt ccagttcttc ttcgtacaat gggaattctg gcgttacttc
     6781 taacaaatct gctgtagcat tcaataacgc gtccaatcct tgtcgagtca ctccagaaat
     6841 tggaaatact gggatgtcat cagcaaattc atcttctttt tccttgttca gctgttcttt
     6901 gaatttcact agattttccg gtgcatccgg catatccatt ttgtttgcca cgatgatttg
     6961 cggacgctct agcaagcgta agttataagt agataattct ttgttgatgg caagatagtc
     7021 ttcataagga tctcgtcctt ccatcccact catatcgatc acatgtaaaa ttactcgtgt
     7081 tctttcgata tgacgaagga attgtgtacc cagcccaaca ccttgcgaag ctccttcaat
     7141 caagcctggc aagtctgctg cggcaaaact tctgccatca cttgtagtaa ccattcctag
     7201 attaggtacg agcgtcgtga aatgataagc gccgattttt ggacgagcag atgaaataac
     7261 ggatagtaaa gtcgatttcc cgacagatgg aaaacctaca agtccaacgt ctgccaatac
     7321 tttcaattct agttcgattt ttcgctcttg tcctggttca ccgttttcag caatttccgg
     7381 tgcaggattc cgaggagagg caaaacggat attccctcgt ccaccgcgtc caccttttgc
     7441 cacaactagt gtttgaccat tttcaattaa atctcctaaa agagcaccag tctccgcatc
     7501 tcggacagtg gttccttgtg gtactttaac ataggtatct tctgaaccac gaccgtgcat
     7561 ccctttactc atcccatttt cacctggctg cgctttgaaa tggcgattga accgaaaatc
     7621 catcaatgta cgtaaccctt catcgacgat caagatgaca tcgccgccac gaccaccgtc
     7681 tccgccagcc ggtccgccgt caggaacgta tttttcgcga cgaaatgcaa ccattccgtc
     7741 tccgcccttg ccagctttca catcgatcgt tacttgatct aaaaacatgg acataattaa
     7801 gtcctcctat tattcattta tttatttgac tgcctgaatt tccacaataa acaaaagtct
     7861 tacttatttt atcggtgaaa tctgattttg tctatcattt cagcaaaaag attgtttacc
     7921 ttcttctttt tttatcattt ggtaaggttt ccaacctttt ttctgctacc aagtataatt
     7981 ttttgacaaa atattctttg cgtagcagaa taacagtgta ttaattgtat taattgagga
     8041 tgccgtttca cagcacccct aataaaggag gaacttttga tatgacagta aaagtaggta
     8101 ttaacggatt tgggcgtatt ggccgtctgg ctttccgccg catcaaagaa gtctcagatg
     8161 atattgaagt agtagcaatc aacgacttaa caagtccaac tatgttagca caattattgc
     8221 aatttgactc tactcatggt acttatcctg gcacagttac tgcaacagaa gactcaatcg
     8281 tagtggatgg cgaagcaact cgtgtttatg cagaacccga cgcaagcaaa atcccttggg
     8341 caaaagaaaa cggtgtagat atcgtactag aatgtactgg tttttataca tctgaagaaa
     8401 aagcaaaagc gcatttagat gctggtgtca aacgagtagt tatttctgcc ccagctggtg
     8461 ctatgaaaac catcgtctat aacgtcaatg atgatacatt agatgaaaac gacaaaatca
     8521 tctcagcagg ttcatgtaca acaaactgtt tagcacctat ggcttatttc ttaaataatg
     8581 aatttgggat cgaagttggt acaatgacaa cagttcatgc ctacacttcc acacaaatgc
     8641 tcttagatgg acctgtaaaa ggcggtaatt tgcgtgctgc tcgttcagct gctgacaata
     8701 ctatcccaca ttctactggt gctgcgaaag caatcggctt agttatccct gagctacaag
     8761 gaaaattaca aggacacgca caacgtgttc cagtcgttga tggttcatta actgaattgg
     8821 tttcagtatt gaaaacaaaa gtaactgctg atcaagtaaa cgaagcaatc aaaaaacaca
     8881 cagttgacaa tccttcattc gggtatgatg accgtcaaat cgtttctagc gatgtcattg
     8941 gaacaactca aggttcgatc tttgacccaa cgcaaacaga agtaacaact gctggtgatt
     9001 tccaacttgt taaaactgtt gcatggtatg acaatgagta tggatttact tgccaaatga
     9061 ttcgcttact agaaaaattc gctaacttgt aatttgatag atcaatcgat aaaagaagcc
     9121 tttgactgga tccagtcaaa ggcttctttc ttttcatccc ctacttttaa actcgttcaa
     9181 tgaacatatt ggcttaaaaa ttgttggact acttgctgat atttttcagg atctgtcttg
     9241 tatgcttccg catgctcggc tccttttaca acatactttt cttttggtcc cttcgttgca
     9301 cgatagacct tatctaacat ttcaaatggt acaaatgtat cttcgtctcc atgtataaag
     9361 agcattgggc gctggttttt cttcaattgt tccactgcat ttgcttcacc gaaaaagtag
     9421 ccagcacgga cttttgtcac tagactcgtt acttgcataa gcggaaatgc tggaagacta
     9481 aacatatcgt ctagctgata agcaagctca tcatgaacgg aagaataacc gcaatcttca
     9541 atgattgctt tcacattatc tggtagtttt tctccgctag tcatcataac tgttgctgct
     9601 cccatgctga caccgtatag aacgatttct tgcgattttc cattgttttc tagcactttg
     9661 tttatccact gtacatagtc tttacgctct ggccatccaa atccaatgta atccccttcg
     9721 ctttttccat gtcctcttgc atcaggaaca aggacattgt aacccatatc atgaaacatc
     9781 tttgcgtaat tggtcatcgt ttcagcgttt cccatatagc catgagccac aagagctgtt
     9841 ttttcacttt tcgtttcagc aggaagataa atcgctgata atttcagacc gtcttttgaa
     9901 gtgatggtcc aatcgcttcg gttttctttt gaactaaacc atttatgtgc atccgtttct
     9961 ttggatgtct gtgttgtacc aggtgtatct tcgtctaaaa agtctttctc agaaggcaca
    10021 acagcataat tataaaaata attccctgca aacactaacg caacaatcag agctaaagcg
    10081 acgcctaaaa ggattttcat ccaaagtttc ataaatcatc catctcattt ctcttttttc
    10141 atataacctt gaattagtat aatcaaagaa tctctactat gcaatgttca tttttataac
//
LOCUS       DACDSY010000103        10139 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDSY010000103 DACDSY010000000
VERSION     DACDSY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.104"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            32..2377
                     /locus_tag="IWK44_002106"
     CDS             32..2377
                     /locus_tag="IWK44_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9550602.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            complement(2714..3715)
                     /gene="ccpA"
                     /locus_tag="IWK44_002107"
     CDS             complement(2714..3715)
                     /gene="ccpA"
                     /locus_tag="IWK44_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP9550603.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            3925..5031
                     /locus_tag="IWK44_002108"
     CDS             3925..5031
                     /locus_tag="IWK44_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9550604.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            complement(5090..5692)
                     /locus_tag="IWK44_002109"
     CDS             complement(5090..5692)
                     /locus_tag="IWK44_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9550605.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(5695..6135)
                     /locus_tag="IWK44_002110"
     CDS             complement(5695..6135)
                     /locus_tag="IWK44_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP9550606.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            complement(6260..7198)
                     /gene="galU"
                     /locus_tag="IWK44_002111"
     CDS             complement(6260..7198)
                     /gene="galU"
                     /locus_tag="IWK44_002111"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9550607.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            complement(7213..8238)
                     /locus_tag="IWK44_002112"
     CDS             complement(7213..8238)
                     /locus_tag="IWK44_002112"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9550608.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            complement(8282..9112)
                     /locus_tag="IWK44_002113"
     CDS             complement(8282..9112)
                     /locus_tag="IWK44_002113"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP9550609.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            complement(9127..10065)
                     /locus_tag="IWK44_002114"
     CDS             complement(9127..10065)
                     /locus_tag="IWK44_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP9550610.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
BASE COUNT         2961 a         2142 c         1865 g         3171 t
ORIGIN      
        1 cgaaataaaa ccattagttg aggtgaccct tttgccacgc aaaaatactc gaaaaaaaca
       61 gaatagaaga aaaaaagaaa aatggtttgt tcctaagatt atttttagag tttttcaatc
      121 attgactgtt tttattactg ttcttctttt gatgttcgca gctctaggta ttggaatagg
      181 cgctggctat tttgcctatc tcgtcgaaga cacacaattg ccgactaaaa aagcactgca
      241 gacagaacta ggaaacatta cagaaacgtc aaagattgtt tatgcagata atacagagat
      301 atcaaaaatc cagacagatc tgatgcgcac gactatttca tctgataaga tttcaccttt
      361 attgaaaact gcaattatca gcacagagga tgaatacttc gataaacacc aaggttacgt
      421 tcctaaagct gttttacgag cattagtatc tgaagctact ggtattggct cttctggtgg
      481 atcaactttg acacagcaat tagtcaaaca gcagatcctt acagatgaaa caacattcaa
      541 acgtaaagca aacgagatca ttctttctgc acaagtagaa aaatactttt caaaagatga
      601 gatcattgct acgtatttga atgtctcacc atttggtcgt aataataagg gacaaaacat
      661 tgctggagtc caggaagctg caaaaggaat ctttggtgta gatgcaaaag atgtgacgct
      721 gccgcaagct gcttatatcg ctggattacc ccaaagcccg atcacgtata gtccatacac
      781 gaacacaggc gcgctaaaag aagatctttc tgccggcctt gccagaaaag attttgtttt
      841 attcagtatg tatcgtgaag gccaaattac gaaagaacaa tacgaagaag caaaagccta
      901 tgaccttaca aaagactttc taccacaaca aatcgcagaa cagactgaca gagaattctt
      961 gtattatact gtgatgaatg aagcaacaag aatcattgca caacaattgg ctgaaaaaga
     1021 caacgctgat atgtctgatt catcggtcag tgacgcttat tatcaaaagg cacagcaaac
     1081 gatccaaaat aaaggatata caatccattc gacaatcgat aaagatgtct atgctgcatt
     1141 gcaaaatggc gttgaaaatt atggatacat gttagatgat ggtgctggca gtcaagtaga
     1201 aaccggaaat gtattgatgg ataacagaac tggccgcatc tacggattcg ttggcggacg
     1261 gaattacagt cagaatcaaa ataaccatgc cttcgataca gaacgtcaag ctggctcatc
     1321 aatcaaacca gttcttgttt atggacctgc aatcgacatg gggcttatcg gatcagaatc
     1381 ccgtgtatca gattatgcaa caacttggca agaaggagaa aatgctggcg agaaaatcgt
     1441 caatgctact aacgaaggat cgaacacctt ccaaaccgtc cgtgagtcac tagaatggtc
     1501 caacaatatc ccggcttatc acttatatca agatgtgttg aattccggtg gatcgaaaca
     1561 atatgcttac gagcactact tggcaaagat gaactacccg gcaaatgaca attggggcgt
     1621 tgaatctgct cctttaggta cagtagatgt cacgacgctt caacagacca acggatttca
     1681 ggctttagca aacggcggcg tctatgaaga aggctatatc atcgattcca tcacagataa
     1741 cgcaggaaat gtaatttata aacacgaaag caactctgtc cggatctatt cggaagcaac
     1801 ggcatcgatc atgaatgaca tgatgcgtag tgtgatcaat gcaaaaatca ctacaccatt
     1861 caaagatgcc atctcaagct taaacggaaa tctaggaaaa gcagattggg tcggaaaaac
     1921 tggttctacc aatgaatacc gagattcatg gctaattgtc tctactccgt ctattaccat
     1981 cagtagctgg gcagggcacg atgacaatac tggaatggac agcaaagcaa ggatacgttc
     2041 ggctaattat cttgccaatc tgatcaatca agcttaccaa gcaaaacctg acatttttgg
     2101 cactgatgag aaattcgaat tatcatctga tgtgatgaag aaaaaagtat ctggatttac
     2161 tggtcaaaca cctggaaaag ttactgtcaa taaaaaatcc atccaaacac caggaaaaac
     2221 agttgaatcg ctatgggcga aaaatggacc gaagaaaagc gcctttaaat ttggcgttgg
     2281 cggtaccgat gaaaactata cagattactg gaacaccgct tcagcttatg caagagcgaa
     2341 tcaagcaact aaagaatcag atgagaaaga tgattgattt aatagaatac acagtaataa
     2401 gggattgtga caatagtatt gtcacagtcc cttatttttt tgatggtggt gaacaaaatg
     2461 cagcatctat acacggattg ttttatatac gataaatgta atttcaacca ctttctctct
     2521 tttttaagac acaaaaaaag gattataaca aaattttgtc ataacccctt ttcccaaata
     2581 aacggtgttc tcctgccaac tccgaatcac gagtcacaat attcatcaaa catgagatgc
     2641 tgttttttga aattgctcct tgtaacgcag agttaaacgg caggcacaca acctctgaat
     2701 ggttgtcctt ttattatttt gtagatcctt tttcttcgat tccataaggc aagataatcg
     2761 ttttttcttc gacttcttct ttattcatca ttttcgtcaa taggcgcata gataccgccc
     2821 ctaaatcgta taatggttgt gtgatacttg ttaaacgagg acgagctact tctgtcagta
     2881 gtgaattgtt acttgtgatg atttcgaatt cttccggtac tttcacgcca cggtctaata
     2941 aaccatccaa aataccgatg gctaattcat catctgaaac atagacagct gttgcaccgc
     3001 tattatggat ccgatcgact agagatagac catctttgaa gttgtattgt gcttcaaaga
     3061 tcaatccttc gctatatggt aggttatttt cgcttaatgc ttctttatag cctttcagac
     3121 gattttgtcc attgatggga tcgatcaacg caccactgac aaaagcgatt tttttattgc
     3181 cgctttttgc caatttgttt actgcatctt ttgttgcgga tacataatca atgtttacgc
     3241 tacctacttg ttcatctgga tcaatagagc cagctaatac aacaggagtt tttgaacgtg
     3301 aaaattctcc acggatttcg tctgtgatat ggtgacccat gaagatcacg ccgtccactt
     3361 gcttagccag taagttattc aatacattga cttctttttg gtcgttgcca tctgaatttg
     3421 ctaagataat attgtatttg tacattgttg ccacatcatc gataccacgt gctaaagaag
     3481 caaagaacat attgctgaca tcagggatga ttacaccaac tgttgttgtt ttcttacttg
     3541 ccaagccacg cgcaactgcg tttggacgat aatccaagcg atcgataact tccaagactt
     3601 tttttcttgt tgctggttta acgtttggat tgccatttac gacacgtgaa acagtagcca
     3661 tcgaaacatt tgcctctctc gctacatcat agatagtgat tgtttgcttt tccatttgtg
     3721 ttctcctatt catattttat atgttttctg aaaatacgtc ttacatttct aaaatagaat
     3781 atcaaataat ctcaagaatt gcaaccgttt tatctctgtt ttcatgaaaa tttttacttt
     3841 tttcagtttt tagaacgttg gtcttttaat aaatcagtgg tacaattatt gaatacaaaa
     3901 ttaatttaga aagtaggttg ccaaatgaat aacgtaaaaa tcaatgaact acgtcaatgg
     3961 atggcacaac accaagtcga attggcttat atcagcgacc ctggccacat cgcttacttt
     4021 tccggatacg aaagtgatcc tcatgagcgt gtactagctc tttttatccc tcttgaacaa
     4081 gatcctttcc ttttcactcc tgctttggaa gttgaagatg ctcaaaacag tagttgggcg
     4141 tatgatgtcc gtggctattt agacagcgaa aacccttggg agatcatcgg aaaagaactt
     4201 cgtgctcgct atggtcagcc tggcaatgta gggattgaaa agaacgcctt gactgtcgag
     4261 cgcttcgatg ctatctctga tatcttgacg ggaacggatt cttacactga tctaacttcc
     4321 atcatccaac gtctacaact gatcaaaact ctagaggaaa aagacaaact gatcgaagct
     4381 ggtaaatggg ccgatgttgc atttgagatt ggctttaaag cagtgaaaga aggcgtgact
     4441 gaacaagcga tcattgcaga aattgaatac caattgaaaa aacaaggtgt ttcacaaatg
     4501 tctttcgaca cattagtcct tgcaggagca aatgctgcaa gccctcatgg tacaccagga
     4561 aatacgaaga tttcaccgaa tgaacttgtg ttattcgatc ttggcgttgt ttggaatggc
     4621 tattgcagtg atgctactcg tacaattgct tatcaaaaac caaccgactt ccaagaaaaa
     4681 atctacaaca tcactcttga agcacagctt gccgcacaag aagccgtccg tccaggtgtg
     4741 acagcaggtg aacttgatca aatcgcacga aacgtgatca acagctatgg ctacggcgaa
     4801 tatttcaatc accgtttagg tcacgggatc ggaacaaccg tccacgaatt cccttccctt
     4861 gtagaaggaa atgacttagt aatcgaagaa gggatgtgct tctctcttga accaggaatc
     4921 tatattcctg aaaaagtagg ggtacgtatc gaagactgtg tatatgtgac aagcgatggc
     4981 tgtgtgccat tcacgactac accaaaagaa ttacttgttt tagaaaaata agagtaaaaa
     5041 aggtgagagc tagtccgctc ccaccttttt attctataaa tttgtgattt tagtgatttg
     5101 tagatgaatc tgtcattgta tcttttattt cttttttagc ttctttcgca tcttcttttg
     5161 ccattttcat catttctttt gagtcatctg caactggttt tgcttctgac attccttcat
     5221 tgacagtatc tcctgcttta ccagcagcgt cttttacatc aatcatgata tcttctgaag
     5281 tatctttagc aatacttcct aattcatctg caggtttttt catatcttct gccgtttttt
     5341 tgaaacgatt agataagtca gctgattgtt ctttaagatt taacaagata tcgtcagatt
     5401 cacttcctgc ttcatctgct gattcactta ctttttgttt gacactacct gctagatcac
     5461 ctgcttgttg agaaagatct tgcgcttttt cttttgcaat acctgttaac tcagtcgatt
     5521 tttctttcgc catatctgtg taatctgaag ccatatccat caaatcatcc gcttgggcag
     5581 ctaattcatc ccgaagttct ttaccagatt ttggtgctaa caataacgcg gcgattgctg
     5641 cggctgtgcc tccgactact gcacctaata aaaatccacc ttttttagcc atttttattt
     5701 tcctcctgtc gtttcagtat gttttttgtt tttaaaaaat ttcatcgctg tcccaccgac
     5761 ttttccggca accgtcgctt tagctgtcgt tttaccgatt ccgccgactt tcgtcgcgat
     5821 gtttttacta gaatgattaa gttctgatac actttcactt aaatcagcaa ctgctgtgaa
     5881 caatggatcg atcgttgcga cttttccatt gatatccaag agcagttcat tacttttgac
     5941 cagtaagcct tctacttgtc tagacaaaag atctacgtct tttgtgacga tttcgatcgt
     6001 cgtatttgct tctgctacag tctgatctac tttttctaaa gttttagaaa cttgcaacaa
     6061 cacacgaacg ataaaaacga caagtatcgc aaaggcgatt gctgcgatca atcctgcaat
     6121 ttctcctcct gacatgatca aactcccttc ttgtctctct tttctataag gatagcattt
     6181 tcgtgttttt taaacaaata aaaacgagct tcaactactt tttattcaat tagcagctga
     6241 agctcgttag aaaaaagggt tattttttct tttcttcttt gatttctgtt attccttttg
     6301 gattatgatc gatcttgctt ccttctgctt ctaatgtttt acctaaagac aggatgtact
     6361 cacgtaaagg tgcacctact tctggatgtg ttagaccata ttcaatactt gttttcataa
     6421 aaccaaattt gtcaccgaca tcgtaacgtt tacctgtgaa tttacgggca aatacgcgtt
     6481 gtgttttatt caatgtatcg attgcgtcag ttagttggat ttcatttccg gctcctggtt
     6541 gttgctcgga caatacatca aaaatttctg gagtcaataa gtaacgtcca ataatagcta
     6601 aatcacttgg tgctttgctt ggatctggtt tttcaacaaa gtttttgaca ttgtataacc
     6661 catcttccaa tacttctcct ggattgataa ttccgtattt tgaagtttca tcatgaggaa
     6721 cttgcataac ggcgatcgta gaagcatgag tacgttcgta atcatcgatt aattgctgtg
     6781 tcaatggtac ttcgtcttcc attatatcat cgcctagcat aacgacaaat ggttcatttc
     6841 caataaaggc tcttgcttga agaacggcat gtcctaaacc ctttggatga gattgacgga
     6901 tgaaatgcaa gttgacatct gtcgtttctt ctactaattt caataattcg gtctttcctt
     6961 tttcacttag gtttgtttct aattcaatat ttgaatcaaa gtgatcttcg attggtcgtt
     7021 tagcttttcc tgtaacgatc aagatgtctt cgatacctga tttcaacgct tcttcaacga
     7081 tgaactggat tgttggttta tcaacaattg gcaacatttc ctttgccata gcttttgttg
     7141 ctggtaaaaa tcttgttcct aatcctgctg ctggaataac tgctttttta actttcatat
     7201 acattcgctc cttcaaaaaa ttgttaatga gaattcattt tctttttttc catcacgtaa
     7261 catgatttct tttgcggctg ttttgatttc tttttgttca tacagcactt catagattgt
     7321 ttgagtgatg ggcatttcga tttccatcat ttgggctagt tccatcgctg cttgggtagt
     7381 attcacacct tcaacgacca ttcccatatt ggctagcacc tcttctaggg gctgcccttg
     7441 tcctaacaag ttgcctgcgc gccagttcct agaatgaaca cttgtgcaag taacgactag
     7501 atcgcctatt ccgcttaatc cgataaaggt cagcgggttg gcacccattg ctacacctaa
     7561 acggctgatc tcagctaatc cgcgcgtcat gattgctgcc tttgcattat cgccaaaccc
     7621 taagccgtga atagcgccag caccaatagc aataatattt ttcaaagctg cccccatctc
     7681 tacaccgatc acatcaggat tggtatagat ccgaaaataa ttattcatga acagctcttg
     7741 tacatatttt gctgcttttg catctgtact tgcagctgtg atcgtagtga tgtcatgttt
     7801 tgctacttct tctgcatggc ttggtccaga aagaacaaca acagcttttc ttttttctgc
     7861 tgggatttcc tctaacaaga tgtcagagat ccttttatgt gttccctgtt ctaatccttt
     7921 acttgcatgg atgatcactg gctttgtatt caatttaggc actagttctt ttgcaacaga
     7981 acgtattgct ttcgttggaa tgacgaatag caccgcatct gcgtctttta ctgcatcttc
     8041 catattggtg aatgcttgga tggaatcggg tagataatga tctggtaaat aacgttgatt
     8101 cgtgtgttgg gtgttgattt cttctacttg tgaagcacga tgtccccaaa tccgaacgtc
     8161 atgtccgttt tcggcgagga cttgtgccaa agcagtaccc caagagcctg gtcctaaaac
     8221 cgctactcta taattcattt taccactcct gaaatttttt tcatttttaa cgctctaaaa
     8281 atcagataat ttgttgattt tttcctaatg agcggtcata atccggtaaa tttccttttt
     8341 ttctgcgcca aataaataga atcaatgtac cgacaaataa cacagcagat aataattgag
     8401 aaatacggat cacacctaaa aagtaaagac tgtctgtacg taatccttca ataaagaaac
     8461 gcccgaaact ataccacatt agataacata aaaggacttc gccttttttc aaaaggtgtg
     8521 gtttctttcg taaaaacaaa agcaaaataa atccaatcaa actccaaagt gattcataaa
     8581 gaaatgttgg ttgacgatag atcccttcga tatacatatt atcaatgatg aattgcggta
     8641 aatgcagact ttctaagaag cttcgcgtcg tgtctggtcc atatgcttcg tggttcatga
     8701 aatttcccca acgaccgatt gcctgtgcta ataatacact tggtgcagcg atatctaaaa
     8761 aagtccacaa agaaataaaa tgatatttag taaaaaaata cagcgtgatc gcgcctgcaa
     8821 tcagtccgcc ataaatagct aagcctcctg aacggatatt gaatatttgg attggatcag
     8881 ctatatactg gggaagatca aataaaacat aatataagcg agcgccaata atggagatag
     8941 gcaatcccca caacataaaa tctgtcacat catctgcttt taatcctacc cgaacagcct
     9001 ctctgctgct aagccacata gcgataatga tacctgatac gatgatcaat gcataccagt
     9061 agataggaat ccctacaagc tcaaaagcaa ctcgattgat ttgtgctaac atattgtcac
     9121 tccttgctag tcgttgcctg agttttcttc aatcaagcga cttaatcttt cttcaaatgt
     9181 tttcgttgcg tcaaagccca ttgtacgtgc acggaaattc atcgcggcta cttcaatgat
     9241 gattgcaacg tttcgtcctg ttttgacagg gatttttatt tgtggaatgc ccacttccgc
     9301 gatttcaacg gtcgtgtctt cagagcccag acggtcgtat ttcttatctt ttgcccatgc
     9361 ttccagataa acagccaact ggacttgcat agagccacgt accgcactcg caccaaaaag
     9421 attcattaca tcaatgatcc cgattcctcg gatttcaatc agatgttcca ggattttagg
     9481 cggttctccg atcaccgtta attcatcttg ctgataaaca tctactcgat cgtctgcgat
     9541 caatcgatgg ccgcgtttga tcaactccaa cgctgtttca ctttttccta tcccactgtc
     9601 accttgaatc agtacaccta atccgtaaac atccactaaa acaccgtgga cacttgttcg
     9661 aggggcaagg cgaccatcta aataactgga taattctcct aataaacgag aagttgagat
     9721 gggagaacgc agtacagcta tcccattttc atttgctgcc tggatcattt cttctggagc
     9781 tggtaatcct cttgaaataa taaatgcggg catatcttca ctacataatc ttctcatgat
     9841 gatcaaacgc tcttctggga tcatccgttc cgcaaaactg atttctttac tgccaaatag
     9901 ctgtaaacga ttatgggaat aataattaaa ataaccagtc aactctagtc ctgggcgtga
     9961 gatgtcccca gttacgatga cacggtctaa gcttttctcg tcgcctgtca cgatttccaa
    10021 agataatttt ttcactaatt ggctaacttt tacaacttct gacattcatg ctacctcatt
    10081 tctattagag tgttctctat cattttatca ttgtctagcc aaaagtacta ccaattaaa
//
LOCUS       DACDSY010000104        10012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDSY010000104 DACDSY010000000
VERSION     DACDSY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.105"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            285..554
                     /gene="rpsN"
                     /locus_tag="IWK44_002115"
     CDS             285..554
                     /gene="rpsN"
                     /locus_tag="IWK44_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9550611.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            569..748
                     /gene="rpmF"
                     /locus_tag="IWK44_002116"
     CDS             569..748
                     /gene="rpmF"
                     /locus_tag="IWK44_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9550612.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            780..1157
                     /locus_tag="IWK44_002117"
     CDS             780..1157
                     /locus_tag="IWK44_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550613.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1178..1327
                     /gene="rpmG"
                     /locus_tag="IWK44_002118"
     CDS             1178..1327
                     /gene="rpmG"
                     /locus_tag="IWK44_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9550614.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1351..2334
                     /locus_tag="IWK44_002119"
     CDS             1351..2334
                     /locus_tag="IWK44_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9550615.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2364..2486
                     /locus_tag="IWK44_002120"
     CDS             2364..2486
                     /locus_tag="IWK44_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9550616.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2504..4027
                     /locus_tag="IWK44_002121"
     CDS             2504..4027
                     /locus_tag="IWK44_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9550617.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4051..4290
                     /locus_tag="IWK44_002122"
     CDS             4051..4290
                     /locus_tag="IWK44_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550618.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4483..4944)
                     /locus_tag="IWK44_002123"
     CDS             complement(4483..4944)
                     /locus_tag="IWK44_002123"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9550619.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5139..5357)
                     /locus_tag="IWK44_002124"
     CDS             complement(5139..5357)
                     /locus_tag="IWK44_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9550620.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5475..7091
                     /locus_tag="IWK44_002125"
     CDS             5475..7091
                     /locus_tag="IWK44_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9550621.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7332..8672)
                     /locus_tag="IWK44_002126"
                     /pseudo
     CDS             complement(7332..8672)
                     /locus_tag="IWK44_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8948..9103)
                     /locus_tag="IWK44_002127"
     CDS             complement(8948..9103)
                     /locus_tag="IWK44_002127"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9550622.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9399..9731)
                     /locus_tag="IWK44_002128"
     CDS             complement(9399..9731)
                     /locus_tag="IWK44_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9550623.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         3317 a         1595 c         1987 g         3113 t
ORIGIN      
        1 cgttttaagt agaggaaaag ctacttaaaa cggtatttgt gtttgattgg actaataaaa
       61 gtgaattctt atttttcaga gcctaaactg catattcttt taaatgattt gattatctct
      121 atgtaccgat aaattcgttt tcaaatagtt atctattcat atgagtttta tttcatgtta
      181 tttattgttg acaagagcaa ctcttgttgg taaagtatca gtgtcaaaac gtaacgatta
      241 cgatttgagg tgtctaaatc aaaataaaag gagtataaag gtatatggca aaaaagtcga
      301 aaattgctaa agcaaagaaa caaatggcga tgattgaaaa gtatgcagat aaacgtcagg
      361 aattaaaagc cgcaggagat cgcaccgctc tcgctaaatt gccaagagat tcaaatccga
      421 atcgtctgag gcttcgtgat cagacggatg gtcgtccaag agggtatatg cgaaagttcg
      481 gtatgtcccg tattaagttt agagaacttg cccatcaagg gttgattcct ggagtaaaaa
      541 aagcaagttg gtaaaaggag gaaatatcat ggcagtacca gcaagaaaaa catctaaggc
      601 gaagaaacgg ttaagaagaa ctcatcaaac tgtagtaaaa ccagaaattt catttgacga
      661 agcgaatggt gattatagac gtagccatca tgtatcttta aaagggtact ataatggaaa
      721 gaaagtaatt aaaggagctt caaaatagtt taatatataa cttttaggag ggaatcaaaa
      781 tggaaattat ttacccacct ttagttgaag aaggattaaa gtattatctt gaaacgaccc
      841 agcaatcgct agataaatgc actttttatc gttcgatggt agagagagga attattactg
      901 aaacgggttt accgacccaa caggcaattg aaaatggatt agtcaaagat tactatgagg
      961 accaaggctt atcatttgat gattttttaa gaatctatcc aatttttgaa gagtatgacg
     1021 aggagttatt tcaatgtatc gatggttatt gggagatacc tgttgatatg aaagaaaatt
     1081 tagtttcaca attggaatct ggcgagttaa cttttgaaga tgcacaacaa attcaagcat
     1141 atcttgaaga tcgataagta ttgaaggagg aagatttatg cgtcaaaata ttattttgga
     1201 atgtgtcgaa actggagaac gtctgtatct caccagcaaa aataaacgga ataaccctga
     1261 acggttagaa ttaaaaaagt attcgccaaa attgcgcaga cgggcaattt ttaaagaagt
     1321 gaaatagcta agtggtaaaa aggtgacaaa atgggcatac cagttacaat cgtttctggg
     1381 tttttagggg ctggaaaaac aacgctaatc aatcaagcct tgcaagtctc accctacccc
     1441 agggaagaaa ttattataat tgaaaatgag tttggtgaag ttgggattga tcacaaatta
     1501 ttagttcata gcgaggaacg ggtgttacaa ctaaataatg gatgtatgtg ttgcagtctt
     1561 cgcagtgatt tattagcaat attgatttct ttactggaaa caatagaaga acatcaacaa
     1621 ccaatttcac aaatcatcat tgaaactaca ggtatagcag atccccaacc aatcattcaa
     1681 acacttttaa cagcgcctca actaagagga agagtatata tcgatagttt attgactgtt
     1741 attgatagtg acagttttga acttattgaa agagaaccac aagcccttaa gcaacttgtc
     1801 atggcagatc gcatttttgt gtctagaaaa caaaacttga atttatctgc actaaaaaaa
     1861 ttacagagag aaatcaaaag tattaaccct ttatccgata ttcgaatgtt cacacatagt
     1921 gaaacgattc aaaaaaagga tttttataat ttgaaaaaat ttaatcatcc cttagttttt
     1981 gaaagtgatt catcagaaaa tcactataat ggcaaatact atcatcaaca ccatcaagta
     2041 catgagttta aatcaattct cttaacttca gatcaagaca taaaaaaaga ttttttactc
     2101 caatggattg actggctgat tttaaccaac caagggagcc tgtatcgttt taaaggattg
     2161 attgctttac aagagcagga ttttatagtt gccatgcaag aggtaaacga acaggttaat
     2221 tttcaataca ccactcgaca aaaagaccaa accactatta ttctaatcgg aaaaatgttg
     2281 gatgaagaaa ggatcaaaca gagttttgat aaattactgg cggaaagcaa ataatgataa
     2341 gtagtgattg ggggtaattt ctaatggaga aaacggatat atctagtgca tatcgacgac
     2401 ttaaaagccc gaatattaaa actagaaaaa gggcattaaa aattatcaaa gaacataaac
     2461 gaaataaaat gaagaaatta gcataattaa ggaaggaaaa agaatgaaga aaatcagtgt
     2521 aggacttatt gggatagcag ctctaggatt attgggagct tgctccagca ctaatgatgc
     2581 aaaagtatcg aatgataaag atggaaaatt agaaattgta acaacattct atccaatgta
     2641 tgatttcacc aaaaacatcg tcggcgatga agcgaacgtg gatttaatgg ttccagcggg
     2701 atcagaacct catgactatg agccctcagc taaggatatg gcaaaagctc atgatgctga
     2761 tgtttttgta tatcataacg aaaacatgga atcatgggta ccaaaagcta aagaaagttg
     2821 gaagaaagct gggcctaatg tagtagaagg aacaaaagac atgattttat tgccaggtag
     2881 tgaagaagaa gatcatgatc acggagaaga agatcaccat catgaactag atcctcatac
     2941 gtgggtttca cccaaaatgg caattaagga agtttcaaat atcaaggatc agttagttaa
     3001 attgtatccg aaaaaagcaa aagtatttga gacaaatgct gaaaagtatt tgactaaact
     3061 gaaacggttg gatgctgact ataccactag tcttaaagaa gcgaaacaaa aaagttttgt
     3121 gacgcaacac gcagcctttg gatatttagc tttggattat ggattgaaac aagttcctat
     3181 tgctggacta agtcctgaag aagagccatc ttctggtcga ttagctgaat taaaagagta
     3241 tgttaaaaaa aataagatca attatattta ctttgaaaag aatgctaatg ataaaattgc
     3301 aaaaactttg gccaacgaag ctgggataaa attggaagta ctgaatcctc ttgaaagtct
     3361 tacaaaagaa caaatggata atggtgaaga ttatgtttca gtaatggaag acaatctaaa
     3421 agcgttagaa aaaacaacta tggttgctgg tgaagaggta gttccagaaa aagaagcgaa
     3481 ggatgaaaag acagttgcta atgggtactt taaagatgcc gatgtgaagg atcgcgaatt
     3541 gagtgattat acaggcgaat ggcaatctgt ttatccgttg ttaaaagatg gaattttgga
     3601 tgaagttttc gattacaaag caaaaatcaa taaagacatg acagctgccg agtacaaaga
     3661 ttattatact actggctata agacagatat tgatactatc aacattaaag acaacaccat
     3721 tgattttgta gtcaacggtg aacatcatca atacacatac aaatatgttg gatacaaaat
     3781 tttgaattat gaaaaaggaa accgtggtgt tcgttttaat tttgaaactg atgatgcagg
     3841 tgctggtcgt tttaaatata ttcaatttag tgatcatgga attgcaccaa gtaaggcaga
     3901 gcatttccat attttctttg gtggagaaag tcaagaaaaa ctctataatg aattgcataa
     3961 ttggcctact ttctatccag cttcattgtc taaacatgaa attgctcaag aaatgatggc
     4021 tcattaaaat tcaatttttg gaggaaatac atggtaagaa aagtattatt gtctatggat
     4081 tccaagaata ttgatgctta tcggcgttat cgatttgcaa agcaaatgag attaaatggg
     4141 agtacattag ctgatattgc tgcaacttta catgttagta ttgacacagt tcatagagta
     4201 ttaaaacggg atcctgattg ttgttttatc aaggtcaata ctggaagaag tagacgttta
     4261 gcaaaacggc ataatagctt ggccaattaa ttgataatac cagagtagat catttttccc
     4321 ttatttggat ttgatctgct ttttattatg tcttgaattg tcaaatcaag cgcaacactt
     4381 tatcaaagaa gacttcataa ggtgttctcc aatctaaaca ctttctaggt ctgttattca
     4441 gtttttcaat catctgattt atcgttgagt tatctaccgt agctatctct ttcgattttg
     4501 gcaagtattc tcgaatgagt ctattcgtat tttaatttgt tcattgttgc caagggcatg
     4561 aggatctgga aaataaaagg gcatattatt caatgcttct gtaactgcga aatgattgga
     4621 gaattctttt cctctatctg gagtgatcgt tttgaccgcc tcatttggaa tactgcttaa
     4681 aagttcaatc attttctcgc tcacataagc ggctttcttt ctggggacct tctttcctgc
     4741 taaaaggaag catgattttc tgtctgtcaa agtcactaaa caagtaccac cagttttgcc
     4801 agctacaata tcggctttcc aatgaccaaa atcacttcta tcatctgcgg attttggacg
     4861 gccatggatc gaatgagtaa ggcaaatctt tccacgagac tcgacatgat tttttgtatg
     4921 tcttgttttt cctcgatggt gcaatgaacg gatcaggcct ttattgccgt gtgagagggg
     4981 ttctgtatcg aaaagatcat tgcaaattgc acgttagatc gtgataaaac taattgaaaa
     5041 gtccatatgt tcatgcttca aacgattcga gatttcctct ggcgaccact gaaaatcgac
     5101 aaataaatga cgaacaattg accaaactca cggatcattt agaatgtgat aacgtccgca
     5161 tttttttctt cgataagcat aatttttttc cgctttggaa ggtgaatagt ttttttcttg
     5221 tttcttctca gttcatgtaa aatagtaaac gggctatgct gaatgtcgtt gccaattata
     5281 ctaatagaaa atccttcttc gtgaagcaga aagattcttt ctcgttcgag tatagtaaga
     5341 tgacgatact ggctcataat tattttcctt ctaggagtgt tctcgacaaa cttatactag
     5401 ttggatatcg attatgactc atttttatgt gttgcactta aagtgtaaat tcaaggtata
     5461 gaaagaggtt ccctatgaaa aaaataccta ttacaatctt aactggctat cttggttcag
     5521 gaaaaacgat acttctaaat catattctgt acggtgatca cggaaaggaa attgctgtca
     5581 ttgtaaatga cataggggaa gtaaatgttg atggggtatt agtcgacaag aaaggtttta
     5641 aaagaactgg agaaaaactt gtttcgatgc cgaatggatg catttgttgt acattacgcg
     5701 aagatttgat tgttgaacta gatcaattag tgcaattttc tgacttgaat caaattgtta
     5761 ttgaagcttc aggaatcagc aaaccaattc caattattca agcaattata ttagctaaaa
     5821 accctttaga cattgatttg acccaacgat tagagattgg tgccattatt atagtagtgg
     5881 atgtaaaacg tttttgggat aatttcgaaa cgggtgaaaa gattcaggaa agaaaagctg
     5941 aaggtaatgt agttgaagaa gaacaggata ttcgtggctt gttaattgat caattcgagt
     6001 gttgtaacat attactttta aataatagtg acttagtgtc aaccaagacg atttacaaaa
     6061 ttaacggtct aattgctaag ttacagtcca atgccaaaat aattaggaca attaaaggtg
     6121 aagtagactt atcgaaaatt ctaaatacaa atctttttaa tatagatgag acttcctatt
     6181 cagtgggctg gttcaaagag ttagagcttg gctatcaaaa tcatattcct gaaacagaag
     6241 aatatggtat ctcaagtttt atctatcgaa atcggttact ccttgacgca caaaaatttg
     6301 cccatcttac ggataaaaat tttcctggca atattacaag agcaaaaggg tttctttggt
     6361 ttgcccaaca tagtgatttt tcgacgatct ttgaaacatc gggtagctat aaaagtattg
     6421 aatcattaaa ttattgggta gctagtttac ctaaaattga gtgtcaaatg attatacaaa
     6481 aaaatcccga attgaaggaa gcctggcatc cgttgtttgg tgaacgcatt aataaactag
     6541 tttttattgg tatgaagatg gatcaacaat gtattaagga acaacttgag ggttgtttaa
     6601 ctgattctct taatgcagat aatttccgaa aaatttcacc atttccatgg aaaaagtcta
     6661 atatgtttag ccagatatcg ttaatgaaat ttttaagaaa agctggtgtc aagataggcg
     6721 atgtcataat tattggcatt ttgattatta cctcttttat tcccttattt gtattctcta
     6781 tagaaaagga gaatgaaaca actgaagatg ctatcaaata tgccatcgtt cgaattgatg
     6841 gcaaggaaat ggatcgcttc gatttggata agatcaatca taaactcgtg aattatcatc
     6901 ctgcgaaggg acaatataat attgttgaga ttaaagatgg aagaattcga gttaaagaag
     6961 ataatagtcc tgatcaaatc gcggttagaa ctggatggat ctcaaaaccc ggtcaaacga
     7021 gtatttgttt accccataag ttagttatta gtattgagaa aaaagaaagc acagattact
     7081 acatttacta atctggaaat agatgaggag gtttttaata agaaaaagat attattatta
     7141 ataatagcag gggctttatt aagtgtgtta agcgcttgtg gacaagaaaa agaagcggtg
     7201 acaacaggca caagtatctt taacgggacc tgcaatggtt tagttaacag tcggttagat
     7261 tacttatttt ggctcttcgt caatgagtgt tggtgaagaa attcaataga ataatcgaga
     7321 tactcacacg gctagacagc tatcttgtct agccgtgttg ttgttctggc tttagtcagt
     7381 tgggtagtat tcttagtttt cttaaaatat tttcctcgat ataaaaagat tcgttctcgg
     7441 tagttttgaa agtttctata gccataggca attcgtttta gtactttgat atggttattc
     7501 aaacattcta tagggccgtt ggaataggga acgcgcaacg ctcgtttgat ttctgtttgg
     7561 tatttcttga aggtcgtgaa cactggttgg tagtgctctg gcaactcctt gtagtcttct
     7621 tttaggagcg cgacgaattc aggaacatct tgtcttctga taacggaaag aaaatcctgg
     7681 tagacttcgt agcctcgctt taaggaatca tcataagata atagccgatc gactatctct
     7741 gcttctgtca gatgatctcg aaatgacgga cgccagtagc gatgctgacc tttcaattcc
     7801 cacgcattct tttgaagtag tttccagtat ttctttaagt gcttcgaccg acgatcccct
     7861 tttttaaagg tcttcattac ttgaattcgg tggttggtaa aagcccgacc aatgtgttgg
     7921 ctaatatgaa agcgatcggc gacgattcgg gcattcggaa aacactcttt caccaatgtg
     7981 cgatagggtg tatagaaatc tgaaaccact agacgcactt gttcacggac ttttataggg
     8041 tagcgcaaga aatagtcgcg taaaggattg agttgccgat tttccacaat atcaatcagt
     8101 tgatgggtct tgccgtccat catgacaaag ctcatggctc ccgagacatt tttcactgat
     8161 ttgaattcat cgaaacacac cacttctggt aacagctggc tcaaagaaat ggttttaggc
     8221 cgaaggctgc gaagtattcg aatgacagtg gtcggagaaa tgtgcttcat acgtgcgatc
     8281 aaagacatag aaagaggttc gcttaatagc tcaagaatgg cttgtttgac tctgcgagca
     8341 atggagcagc gacgatcact aactgaatct tctgctaaaa aagtctttcc acagtgttta
     8401 catttgaagc gctgtttgtt aatccgcaga atggtcttgt agttactgac atcattcaat
     8461 aagatggata cttttttcca gccctattta atgatcgttt gttcattttg atagtggcga
     8521 tgaaagcatt cctccggttg ataggtcaat gtgccatcaa aaacaaaaca tgtttcgtca
     8581 ttgattcgct ctttgcgaaa ggcatcttca tgaaagataa gatttaggtc tagaatatct
     8641 agtaactctt tggtaaactc tgaataggac atcttgaaac actcctttga atggttttcg
     8701 tcgactttat tctaaaggat tttaaggtgt ctgtttgcgt ttattagaaa aaagaaaaag
     8761 tattggcaac agaaggcgtc accaacacta gatattatag atcccttatt ttcttcgctt
     8821 caaattgtca aggagtcatc ataaattttg tatggattct tttgtttggg tagtagtctt
     8881 ctaaataatc ttggattaac accttcatct ctattttact tttatatttg ttggagtcga
     8941 ttaattctca tttgattgat cgatgaaacg cttcaattac tgcattgtca acaggagtcc
     9001 cagtttggct cactgaatgt gtgatgtggt ggtcgttcaa cagctcttga taaataaaag
     9061 accgatgctg gctacctcta tcggaatgaa ttaccgtagg caattctcct taataaattt
     9121 gtaatggacg gacgacaatc tcttttttca tgtgtgaatc taccatatag taacgtagaa
     9181 tacgagttgc taagtcaata taggtggaca aatagaggcg accgtcatta catggaatgt
     9241 aagtaatatc catcacccaa acagcatcaa tttggttctg ttcaaattat cgattcaaca
     9301 tttttttacc atttctttga ttgttttttc tctaccatga gaagatttgc gtcgatgaaa
     9361 tcctcttgtg cataaattca tttcacgcat aagaacagct acaacacgtt tattgatttc
     9421 gattccttca tcgtaaagtt tttgagtgat ccctggagat ccgtatcgat ctttgtgttc
     9481 gtagaacact cgtcttcctc ttttagtcgt ttattttcct gttcaagaat gtagaaatct
     9541 gctttatcag gtaatctttt tccttgtcca gcaaaagcat ttttcccgta tcgacgatat
     9601 tctgaaaccc atcgggaaat tgttggacga gagatattgt attcttcagc aattttcttg
     9661 gtagttactt ttttctcaag gatatcctgt gaaatcttta gtttaaattt acgatcatat
     9721 gtttttctca atagagttca ttccttccgg taacgtaact gatagtcaag aaaagtgtat
     9781 catagcccaa cttagtatca gggtttgtca actaaactgt ggaagttgat agataagagt
     9841 ttttaacaac caaaattctc ttgactattt ttgaaatcag acaaattttg attggacaca
     9901 tagttgaata aatctaccgt cgttacggaa ggtaaatcgc atgcttttca attctagagg
     9961 agaagtcctc gttgaagtac catagcccag ttctggattc gacctcctgc cc
//
LOCUS       DACDSY010000105         9884 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDSY010000105 DACDSY010000000
VERSION     DACDSY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.106"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            353..1345
                     /locus_tag="IWK44_002129"
     CDS             353..1345
                     /locus_tag="IWK44_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9550624.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            1361..2245
                     /locus_tag="IWK44_002130"
     CDS             1361..2245
                     /locus_tag="IWK44_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9550625.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            2294..3901
                     /locus_tag="IWK44_002131"
     CDS             2294..3901
                     /locus_tag="IWK44_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9550626.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            3902..5386
                     /locus_tag="IWK44_002132"
     CDS             3902..5386
                     /locus_tag="IWK44_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP9550627.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            5563..6555
                     /locus_tag="IWK44_002133"
     CDS             5563..6555
                     /locus_tag="IWK44_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9550628.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            6607..7476
                     /locus_tag="IWK44_002134"
     CDS             6607..7476
                     /locus_tag="IWK44_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9550629.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(7896..9461)
                     /gene="guaA"
                     /locus_tag="IWK44_002135"
     CDS             complement(7896..9461)
                     /gene="guaA"
                     /locus_tag="IWK44_002135"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9550630.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9516..>9884)
                     /locus_tag="IWK44_002136"
     CDS             complement(9516..>9884)
                     /locus_tag="IWK44_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9550631.1"
                     /translation="FKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQ
                     ETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGY
                     RNFYNFRARIYLQQGLIFEN"
BASE COUNT         3077 a         1674 c         2128 g         3005 t
ORIGIN      
        1 taaacttact ggtgagtggt cttgaacata cttttaaatc acattttact gagttttatt
       61 ggattttata attcattgga aacctcgtac ttaatctgtt tttatctatc gactagaatc
      121 tgtatatatt ccagctatca atgaggccgc agaaaagggt gcttaaaaat aatctgttgt
      181 ttaaaatatt attatcagaa tacttagata caaaattctg gcacaaagta aaaaaaatat
      241 tgacttaaaa aaatggatat ggtaatctat aaacgaaaat agtggtaacg tttccaaaat
      301 gcatcttcgt taaattttta tttttttaaa cgaaaatgga aacgtttcta aaatgtattg
      361 ggaggagatt gaattggaaa agacattggg accaggtggt gagataaggg cggtacaacg
      421 ggataaaaaa aatatttcga agtgggaaaa attaaagaaa cagttgcggc gtgataagtg
      481 gatgtacttg ttgctcttac caggtatcat catgacgttt atctttaagt atgtgccaat
      541 gtacggtgct gtgattgcat ttaaagacta taatcctatg ctaggaattt tggaaagtcc
      601 ttgggtagga ctagatcact ttagggaatt tatcagttca ccaaatttta cgcttttgct
      661 gagcaacaca ttaaaaatta gtatttatgg tttattgtta ggtttttttc caccaattat
      721 tttagcactg agcttgaatc aaattatcaa taaaaaaata aaacagaaaa tccagttgat
      781 tctatatgcg ccgaatttta tctcagtagt tattattgtg gggatgctgt ttttgttcct
      841 atccgcaaat ggacctatta atgggttagt agaaaaggtt acggggcaac caattatgtt
      901 catgtctaat ccagcttatt tcagaacgat ttatatttta tcgggaattt ggcaaggaat
      961 cggctggtca tccattcttt acaccgcaac gctggcggga gtcagtaccg atttatatga
     1021 ggcagcaaat atcgatgggg caaatatttt acaaaagatt tggcatatcg atttgcctgc
     1081 attgaggcct gtgatggtga tcagcttcat cttggccgcc ggcggtatta tgaatgtagg
     1141 atatgaaaaa gcctacttga tgcaaacttc aatgaacatc cctgcctcag aaatcatttc
     1201 cacttatgta tacaaagtcg gcttgcaatc aggtgattat gcctactctg ctgctgttgg
     1261 tctgttcaat atgattatta atgtcgtttt attactgatt gtcaacaata taaccaagcg
     1321 gctaaacgat ggtcaaggct tgtaatgaaa ggagaaaatt atgaatagtg ctttatcaaa
     1381 aatcgaccgt caaatcttat ggttgaatcg agtgattgta acttttttag tcgtcatcac
     1441 cattgttccg ctgatttatg tactggtagc ttcttttatg gacccagcta ctttgatcaa
     1501 taaaggtata agcttcaatc cgaaagattg gacaatacag ggatatcaac gggtatttgc
     1561 agatgattcc attttacgcg gttttgtaaa ttcgttgttt tactctttta gcttcagctt
     1621 attgactgta tttattacga tgatcactgc gtatcctttg tctcgaccta acttggcggg
     1681 gaaaaagatt attatgactt tttttgtcat tacaatgttc gtgggtggtg gcttgatacc
     1741 tacctattta ttggtaaaga acttgggcat gttagatagt gtatgggcga ttatcgtacc
     1801 aggtgctata aatgtgtgga atattatctt agcccgtacc tattttagtg ctttgccaaa
     1861 agaactttct gaagctgcta ctatcgatgg cgccaatgac ttgcaaatac tgttcaaagt
     1921 catgcttcca ttagcgaaac caattatgtt cgtattgttt ttatatgcct ttgttgggca
     1981 atggaactca tactttgatg ccatgattta catcaaagat cccgacttac agccgttgca
     2041 actcgtatta agaaatattt tgatccaaaa ccaaccttca caagacatgg tgggagccaa
     2101 tactgccatg gcagaaatga aacaaattgc tgagttgatc aaatacgcaa cgatcgtcat
     2161 ttctagtctg ccattaatca taatgtatcc tttcttccaa aaatattttg ataaaggtgt
     2221 tttagttggt tctgttaaag ggtaagtatt gttcactatt tgtatgaata aaaattataa
     2281 aggagatttt aagatgaaag caaaatggat gctgccaatc gtttttactt ccgtactaat
     2341 tttgggggct tgtggaggct caaaagaaaa gagtaaatca agccctgatt atgaactaga
     2401 aaatgtctct tttccattga aagaaaaagt atctttaaaa atgatgtctc aaagtgcccc
     2461 tttggctccc aatgatccta atgacaaatt gattttccaa cgattagaag aagagaccaa
     2521 tctccatatt gattggacaa actacagtag tgattttgcc gaaaaaagaa atttggatat
     2581 cgctagtggt gatttaccag atgccatctt caacgccgct gcaggtgact atgatttatt
     2641 aaactgggcg gaaagtggcg ttattgtacc gattgaagat ttgatcgaag attatatgcc
     2701 aaatctgaag aaaatttttg atgaaaaccc cgaataccga caaatgtcta ctgcacctga
     2761 tggtcacatt tactctatgc cttggattga agaattaggc gaaggaaaag agtcgattca
     2821 tacagtaaat gggatggctt ggatcaacaa ggagtggttg gataatttgg gtcttgaaat
     2881 gccgcaaacc actgatcagt tgatgactgt gttggaggct ttcaagacgc aagatccaaa
     2941 tggtaatggt gaagcagatg aaattccttt tagctttatt aacaatggag gaaacgaaga
     3001 cttaaaattt ttaatggggg cattcggtct aggtgataat gatgaccact tagtcgttga
     3061 taacgatggt caggtacaat ttactgctaa caaagaaggg tataaagaag ccatcaaata
     3121 tttcaatgaa atgaataata aaggtcttat tgataaagag gctttcgaac aagattggaa
     3181 tacgtatgtt gccaaaggga aagaacataa gtatggcgtg tactttactt gggataaagc
     3241 aaatatcacg ggtgcagatg atagttatga cgtcttgcca gtcttagcgg gaccagatgg
     3301 aacgaaacat gttactcgta cgaatggcat gggtttctca agagatcgtt tcgtaattac
     3361 tagtgcgaat aaaaacttgg aacttacagc taggtggata gataaaatgt atgaaccgtt
     3421 acagtctgtc caaaataact ggggaaccta cggtgatgaa gcacaggcga atatttttga
     3481 attaaaggat ggtatgttaa aacatttgcc tttagagggg acagcaccgg gggaactaag
     3541 acaaaaaacc gaagctggag gaccattagc agttcttgat tcttattacg gcacagtcac
     3601 tactatgcca gatgatgcga agtggcgttt agatttgatg catgacacgt atttgccata
     3661 tattacagaa gacaatatct atccacgtgt ctttttagaa aaagaagaaa cagatcgatt
     3721 agcaaaaatc gaaacggata tgtgggatta catctatcgc aaaagagcag agtggatcac
     3781 cggcggtaaa atcgatgaag aatgggaaga ataccttgct gaattggaac gtgtaggttt
     3841 ggaagaatgg attcaaataa aacaaactgg ctatgataat tttttgaagg gagcacaata
     3901 agtgttagta gaagaaacgg cgattttgga taaagcaaat cgatttattg aaatgatgcg
     3961 aggaaaagtt aaccctcagt atcgacaaaa ctatcatttt tccgcaccca tcgggtggat
     4021 aaatgacccc aatgggttta tatattacaa agggcattac catctcttct atcaatacta
     4081 tccttacgat agtcgatggg ggcccatgca ttgggggcat gcgcgcagta gtgatttggt
     4141 ccattgggaa gatctaccag tagcgttagc cccagatcaa ccttatgaca gcggcgggtg
     4201 tttctcagga agtgcaattg aaagagacgg aaaactatat ttattttata caggctgtca
     4261 agaaattgat ggtattagcc atcagcatca atgtttggcg gtctctgaag atggtattca
     4321 ttttagcaaa tacagtggta atcctgtggt agcagaaaaa cagctaggtg aaaatgggct
     4381 aatacaagaa tttcgtgatc ctaaagtaat tgttcacaac gatggttact atatggtagt
     4441 agctactaaa tctacacagt ctactggtag aatagtgatg tttcaatcgt cagatttgta
     4501 tacttggcac ttttactctg tgctgctaga agggacttca gagcaaggaa taatgtggga
     4561 atgtcctgat ttattccatt tagacggacg agatgtctta atcatgtctc cgatacaaat
     4621 caagaagaac ggactggaat atcataatat cagttctacc atggcttgta ttggtgaggt
     4681 aaactgggaa acaggtcaga tgatagttga taatttccat gaaatggatt ttggaatgga
     4741 tttttatgca ccacaaacaa caagagatcc taatggtgga cgggtgatga ttgcttggat
     4801 gcagatgtgg gatcgtacat tgcctacaca tgagttaggc catctttggg caggctctat
     4861 gagtttgccc cgagagttaa gagtgaaaaa aaatcgattg gtacaaaaac ccatctcaac
     4921 tgtatatagt catttagttt atcaacaagg cagtgaaaat attgcagtga ctgacacatc
     4981 agtcgtattt cgaaacgcgc tgtctgattc ttcatattta catctagtcg cagatttgga
     5041 gaaagctgaa gtttttacaa ttgaattact gcggtctaag acggattgtc tcaaactagc
     5101 ctatgacact gctacacagg tttttgaatt ggatcgtcag aaactaggaa gagagcttac
     5161 aggggcggaa gaagaccctc tgaatgtacg aaaagttgaa gtacctttga ttgataatca
     5221 gctcgttctt gagattttta gagatacctc atcaattgaa gtctttatta atgggttaga
     5281 agttatgacg gctactttct atgagctgga aaaagctcga gatatcttgt ttcattcgat
     5341 cggagaaagt cgtattcaag cttttgaaac agcgagtgtg aaataaatgt ttgattgctt
     5401 aggcgatgaa tcaattattt aataggtttt gaaggataat gtttttagaa aagaacagat
     5461 agctagattt cccatggatt tgctattagt attttgaaaa aattagtaat ttatgacaaa
     5521 attcattata ctaaacttgt gcagaaatgt gagggatcaa ctatgaaagc aacaatgaaa
     5581 gatgtcgcaa aattagcagg tgttggagta ggaactgttt cccgggtatt gaataacggt
     5641 tctgttaaag cttctactag agaaaaagtt gaagcagcaa tgacagagtt aaactatcaa
     5701 ccagattttt atgcccgggg gttaaagatg aagcatacga acacggttgc tttgattcta
     5761 cccacggtat ggcatccatt ttttggcgaa tttgcctacc atgtagaaac agcattagaa
     5821 agacaaaact acaagcttta tttgtgtaac tccagtggta atagtgaaaa agaacaagaa
     5881 tatatccaga tggtcagcca aaataaagta gacggcatca ttggaattac ttattcagat
     5941 atcgatgaat acatttcgag taatctgcct tttgttagta ttgaccgtta tttcactgaa
     6001 gaggtgattt atgtgaccgc tgacaatgcg gccggtggac gtttggctgc aaatgaactt
     6061 tatcgccgag gatgtcagaa tgtagcatat atcggtgggt accaagaaac accgaatgaa
     6121 acgaagagtc gtcgaaaata ttttgaaatc gagtgccgtc aactggggat ttcctataaa
     6181 gcgttagata tgttagaacc tttagaccat ctctcagaac gtgtaacaaa ttttttgtta
     6241 gagaatcccg atatcgatgg gatttttacg atcaacgatc caatggcatt agctgtcatt
     6301 gatatgcttg aaaaaatagg gaaaaacgta ccagaggatg tccaagttat cggatttgat
     6361 ggtcaaaaga tgacagaagg agatccttat ctagtctcaa cgattgccca acctacaaag
     6421 caaatggcgg aagtggcagt ggcaaaatta attgctgtca ttaatggaga gacagtagaa
     6481 aaacgtaccg tattgccgat tgaatttggc gaaggtgaca caacgaaacc tttagtgaga
     6541 aacgattttg cttagtgcaa atacgtttct ttttttgaaa atgagtgaat gaaaggtgta
     6601 gataaaatgt tatatggtgg aatagaagcc ggtggaacaa agtttgtctg tgggattggt
     6661 aatgaacaat tacaagtgat agaacggaaa agttttccta ccacaacacc agaagaaacg
     6721 atggagcaag tgtttgattt ctttgataag tacaaaggaa atatcgcatc aattggtgta
     6781 ggagcatttg ggcctattga tattcaaatc gaatcaagaa cttacggata tatcaccaac
     6841 acaccgaagt tagcttggca aaattatcat tttgtaggag ctttggaaaa cgccttgtcg
     6901 gttcccatct attggactac cgatgtaaat gctgcttgtt atggagaata tatggcaggg
     6961 tatggaaaag gtaaaaatag tgttctgtat tacaccgttg gtacaggtgt tggtggtggt
     7021 gctgtagtgg atgggaaaat agtcgcaggt tttagtcatc ctgaaatggg acatatgctc
     7081 ttacaacgtc ataatgaaga tcaatttgaa ggaatctgtc cttttcatca tgattgtttg
     7141 gaagggttag catcaggacc tgcgattgaa aaacgctttg gaagaaaagc ccagactatc
     7201 tcatttgatc atccttattg gacgattgag gcagattatc ttgctcagtg tgtgtataac
     7261 acaacgttac tatatgcacc ggaagtaata attttaggcg ggggtgtaat gaaacagccg
     7321 caactactcg agttgattcg ccaatcgttt aaagaaaaaa tgggttccta cgtggatacg
     7381 cctactctcg aagaatatct actaactcca aggcttgctg acaatgcggg cttgattggc
     7441 tgcctagctt tagcaataaa gaaactcaat cattagaata gtatttatta gaactattca
     7501 taacataccg gaagtttaaa agggtattct gaaacttaaa tagtctcaat tcattcttct
     7561 ttcttgagaa tctgagtaga gacttttatt ctgtttcaaa ttaatataat tacgaaatga
     7621 tattgagtac aagaaagttc tttttaccag atcttttaaa tcgaaaaatc tgatatatat
     7681 atttatagta ctaaaaatct gattagataa aaagctaatt caataggaaa ataaaggtag
     7741 tgaataaaag caaagccctt gttagtagtt aatctaacaa gggctttggt agggaaaatg
     7801 gtaggaaacc atcttatttt ttacgaaaat caggaacttt agtaattgaa taatgttgat
     7861 ttataggtat tcctcatcca tattatgttg gctctttatt cccactccac tgttgcaggt
     7921 ggtttgctgg taatatcata cacgatacgg ttaacgtgtg cgacttcatt tacgatacgg
     7981 acactgattt tttgcaagac ttcccaaggg atacgggcaa agtcagcggt catgccgtca
     8041 atagaagtga cagcacggat acctacagtg tagtcatacg tacggccatc tcccataacc
     8101 ccaacagagc gaatgcctgg taggacggtg aagtattgcc agatgtcgcg atctaaaccg
     8161 gcattggcga tttcttcgcg caagatagcg tctgattcac ggacgatttc taatttttct
     8221 tctgtcaatt caccaaggac acgaatacct aagcctggtc cagggaatgg ttggcgccaa
     8281 acgatgctgt caggcatgcc taattgggta cctaattcac gtacttcgtc tttgaacaat
     8341 gtgttcaatg gctcgatcag cttgaattgc atgtcttcag gtaatccacc tacattgtga
     8401 tgtgatttga tggtttgggc agtttcagtt cctgattcga tcacatcggt ataaagggtt
     8461 ccttgtgcaa ggaaagaaac gccttcttca ccggcaagtt tggttgcttc atcatcgaag
     8521 acatatacaa attcatttcc aatgatttta cgtttttctt ctggatcaga cacgccagca
     8581 agttttgata agaaacgttc tttggcatcg acttttataa tgttcaagcc aaattttccg
     8641 ccaagacttt ccataacttg ttctgcttca cctttacgca acaatacgtg atcaacgaag
     8701 atacatgtta attgatcgcc gatcgctttt tgtaaaagaa caccaacaac agaggaatct
     8761 actccgcctg aaaggcctaa taaaactttt ttgtcaccga cttgttcacg aatttttgcg
     8821 atctccatgt cgatgaagtt atccattgtc cagtcaccag tacattggca gacatctagt
     8881 gtaaaatggc gcaacaagtc atttccatat tcggaatggc gaacttctgc atggaattgg
     8941 attccgtaca ttttgcgttc tgcattttgg atcgatgcga tggggcaatc tttgctggta
     9001 gcaacgacat cgaaatcagt cggtgcttgg gtaactaggt caccatgact catccaaacg
     9061 gtttgttttt caggagtgtt ttggaaaagg gccgcttctg cagaagtgat ttccaattct
     9121 gcttttccgt attcacggtt tttggctggt ttaacttttc cgcctaattt ataagtaatc
     9181 aactgcatac cgtaacagat tcctaaaact gggatcccca aatcaaaaat cttttcatca
     9241 ataccaaagg ccttctcatc ataaacactg tttggtccgc cagaaaggat gattccttta
     9301 gggttcattg cctttacttc ttctgcggta atgcggtggc tcatcaactc tgagaaaaca
     9361 ccgaactcac gaatacggcg agtgatcagt tggttatact gactaccgta atcaagtacg
     9421 ataatttttg gctcttcgtc aaatcgtgtt ggtgaagaca aatttgaata aaacttagtc
     9481 cgatatgcgg cgcgttctgt gcgccgcata tttttttagt tttcaaagat cagaccttgt
     9541 tgtaaataga tccgtgcccg aaagttataa aagttgcgat aaccaaacgc aattcgttta
     9601 atcagcttga tcttgttatt cagcccctca gtgacgccgt ttgagtggga caccttaaag
     9661 gcatttgaaa tacccgattg gtagcgatgg aagatcgtga atttcttttt aaacgactga
     9721 ggtaacgtag atgaaattgt tttaagggtt tcttgaaagc cttccgtacc tcgtttaacg
     9781 aaataatagc gtaataattg acaaaaatga tacgcagttc gcaattcttc gttgtagctt
     9841 aacagctcat caacgatttc tgtttgacta attggtcgtt taaa
//
LOCUS       DACDSY010000106         9782 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDSY010000106 DACDSY010000000
VERSION     DACDSY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.107"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(180..794)
                     /locus_tag="IWK44_002137"
     CDS             complement(180..794)
                     /locus_tag="IWK44_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP9550632.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(941..1624)
                     /locus_tag="IWK44_002138"
     CDS             complement(941..1624)
                     /locus_tag="IWK44_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9550633.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1733..2191)
                     /locus_tag="IWK44_002139"
     CDS             complement(1733..2191)
                     /locus_tag="IWK44_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP9550634.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2275..4038)
                     /locus_tag="IWK44_002140"
     CDS             complement(2275..4038)
                     /locus_tag="IWK44_002140"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP9550635.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4259..5719)
                     /locus_tag="IWK44_002141"
     CDS             complement(4259..5719)
                     /locus_tag="IWK44_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550636.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5927..6853)
                     /gene="glsA"
                     /locus_tag="IWK44_002142"
     CDS             complement(5927..6853)
                     /gene="glsA"
                     /locus_tag="IWK44_002142"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP9550637.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6876..8303)
                     /locus_tag="IWK44_002143"
     CDS             complement(6876..8303)
                     /locus_tag="IWK44_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9550638.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8334..9548)
                     /locus_tag="IWK44_002144"
     CDS             complement(8334..9548)
                     /locus_tag="IWK44_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP9550639.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2898 a         2035 c         1622 g         3227 t
ORIGIN      
        1 ggcatttcta tttccttacc atctaaagta acagtagtaa cgacaaaatg gtcttgcaat
       61 ggcggaactt ctaagtactg cccttgcaga aaatcattca ctgtttcata ttcttttttc
      121 atttcttttg aatctgtcga tggtttatag cttattttta acataactag tgctcctttt
      181 tattcgggac gtgcaatcgt gaaaatttct cctattgctg cataatcatt tcctgccaaa
      241 cggcttacag cattcaattt tcttggatcg atccttcctg tcttatcatc atagactgtt
      301 tcatttaaat gatatttctg tacttctagc aaaaataaat cagctgttac tttattttta
      361 ttccgtatct cgacatgttc atataaggat gtttcaaaac gaacttttgc ttctgtcaat
      421 gctggtactg aaatgattac tgaatcagtt aaagtcaagt cagtcaggta cagttcgctt
      481 tcctcattag gcagattggc tgctgtttta tttacttctt ctacgttgtc ctcatcaagg
      541 atatggataa cagcttctct tgtctcaaga atattctttg ctgtgtcttt tactgcatct
      601 tgttttctct ggatcgccaa ggataagatg ggtcgattcg aactgacgat attgaagtaa
      661 ctgaagggtg cgatattcac tatatctttt gctgatttag tcgttactac tgctactggt
      721 ctagggatga ctgaaccaat cagcaacttg taattttcac gttctgtcaa ttctgaagga
      781 ctccgttcaa acatggtttc gccttctttc tataaaattc gttaaaaagc agatcttgaa
      841 gacaaaatga aaagtaatgc ttttttcatc ttcttagaat ctgctttaaa ctatctatca
      901 atttttcttt tttgtcaaga atctgtgccg tgcgtgtttt ttagttattt gctttacttg
      961 cggcttcgac atatctttta gtgatcaatt gcggacgggt gataatagat ccaactacga
     1021 ctgaaaaagc acctaattca tatgctttct ctacttgttc aggagagtca gtatgtcctt
     1081 ccataattac tgggatatcg acttgttcta tgatttgatt caataaatca aaatcaggtg
     1141 tctgcgtctt tcgtgaatat tctgtatagc ctgctaaagt cgtagacaaa agatcaaaac
     1201 catcttcttg cgcttgaagc gcttcctcaa aagtcgaaat atctgccata gctaaccgac
     1261 cgtgttcgtg aatcatttgg acaagttcag cagttgtttt ttctgtcgga cgtttacgag
     1321 aagttgcatc cagtgcaatg atttctgctt gcgtctccaa aagttcttcc acttcttttt
     1381 ctgtcgcagt aataaacact tctgacccat cgtaatctct tttgataatc cctattaccg
     1441 gtaaatcaac gattcgcttg atttcattga tatcttctgt cgtatttgct cgaataccaa
     1501 ctgctccgcc ttcttttgca gccaaagcca ttctccccat aataaaagaa ctgtgtagcg
     1561 gctcgttttc caaagcttga caagaaacga cgactccgcc tttgatttgt tccagtgtat
     1621 ccacaatatc tcctccagta atgttatata aaaatacagc ttcatataaa aatgtaaccc
     1681 ttttctattc aggagacaat aatgagctat ttctttcagc agacctttct tactaaaaga
     1741 aaattacttc aaattcttcg tatacgacca gcttctcttc tgtaaattct atgttcaacg
     1801 cttctttata agcatgttta aaaaactcag catggacttt ccgccaataa gctaatgtcc
     1861 gatccccttc tccttctttc caagcatgct ctttcgtgac ttcactaaaa ggcaagatgg
     1921 tcacatttat atttttgatg atacaaactg gttcattttt tccattcagg accacgtcta
     1981 attgaccaac ttttggtaaa ggctcattct cttgtttata cagatcgtaa ccacttgatg
     2041 taccagtctt tatctttttt acaactagtt cgctcaactc gtctgccatt ttttctgtat
     2101 ccccaaatga aaagaaacct aatttttctg attgataact aggatatttt tcttggaact
     2161 ttttgacata ttcttctact tgctgataca taacatccac ccctttgctt tttataatga
     2221 aaaaaccatc cagatactag tataaacaat tatctggatg gtgagcataa tgaattattg
     2281 aatattcaag acacgcagac tatcttcaac gatttgtttt ggtgttaaat gataacgttc
     2341 ataaagctgc cctacaggaa cgctatccgt gaattctttc tctgcaccaa aatttaatac
     2401 gttcattgaa gaatcaccat aataacgact gatattttcg ccaaatcctc ctgacaaact
     2461 accatcttct aatgtgatga ctaattcgtg tgtttctttt aatttatcta atgtttctac
     2521 gtcattttct gtgatcgttc ctggattgat caacgttgct tcgattcctt tttttctaaa
     2581 agcttttact gtttcttttc ctaattcaag aaaaccgcct aaagctaaaa ttgctacttt
     2641 tcctccatta tttactgttt gataggctgg aatactataa tcatttgcga tagctgtacc
     2701 atgttctaca ccatgtgtag gaattcggat tgctacaggc tgcgctgttt gttcgaacgc
     2761 ccaatctagc atagagatca tatcctcttt tgttgctgga gcaagatatt tgatgttcgg
     2821 gatacttgat aagtaaggaa tgtcaaatgt tccttggtgt gttcggtcac tagctgagat
     2881 cgttccgcca ccgactaaaa taatcactgg attctcgttg atcgctaaat catgtgataa
     2941 ttgatcatat gcccgttgca agaaagtact tgattggaag acgactggac gtactccctg
     3001 agaagccaaa gcagaagcga aagtgataga gtgctgttca gcgatcccca cgtcaaaata
     3061 atgatctgga tgtttttctt ggaatgtttt cagtccaaag gcgcctggta tcgctgcatt
     3121 gactgcggca ataggagctg tacttgtttg cattttctta tccaaataat ccatgatgat
     3181 catattaaat gtttctccag aagttacttg ctttgcctcc ccggtggaca gatcaaatgg
     3241 tacatgccaa tgatatttca ttttattatc gattgccggc tgataaccgc gacctttttc
     3301 cgtatggacg tgaagtacaa tcggatgatc aatatctttt acttcttcaa ataaacggat
     3361 caccgtttcc agatcgtttc cattatccac gtaacgataa tcaaatccca ttgctttaaa
     3421 taggttattg ctgcttttac cgttggtttg acgaagctca gctaagtttt tatataaact
     3481 gccatgattc tcatcaatag acatctcatt atcgtttact aatacgatta gattggattg
     3541 taaaacacca ccattgttca atccttcaaa agctaagcca ccacttaggg agccgtcacc
     3601 gataaatgct accacatttc ctgattcttt cttcagatct ctcgctttag ctaaacctgt
     3661 tgctaagctg atagatgtag atgtgtgccc cactgtaaaa tagtcatgtt tactttcatg
     3721 ttggttggaa tatccgctca cttcacggta atgtcctttt tcaaaaccat tttttcgacc
     3781 agttaaaagc ttatgaccat aagactgatg ggaaacatcc caaacgattt tatcctttgg
     3841 tgaatcaaat acataatgaa acgcaatcgt taattctact acacctagat tcggaccaac
     3901 gtgcccacct acatcgctgt ccttttgcaa gattttttgg cgcatctctt ctgccaactg
     3961 tttcatttcc tcaacagata attttttgac atctgcgggg ctattgattg catccaatac
     4021 atgtaatact gtactcataa tacattctct cctccgtact atcttctttt aatgaaaata
     4081 gatttgttac actttatcta ttctactact tagagttgac tctaagtcaa tagcatttta
     4141 aaaaaatttc agagaaagct tgttatataa gtagatcttc gcacgaatca ggactcaaat
     4201 aaaaaccttc tacagacaat taaaaagctt tgtagaaggt ctcctaatta aggtctcatc
     4261 aagaaatatg tttttttaat aggtgaagtt cctgagcaat atgatacaaa tggatgtttg
     4321 caccttttaa tgagattgga tagatctttg tcttttcagt ttctaatcca ttttgatagc
     4381 tagcagcatc gcacaaaacc actctttttt tcgcacatag acattcatca ggcaattctt
     4441 ctaatgaacg aaataaaata ggatttattt cagcgatgcc ttcttccgca tacaaatgaa
     4501 attgggttcg ataaaaaaat tgtttgactg aactttctga aacaacaaaa atttctattt
     4561 cctgtctccc cgcttgttca ctgttgatca aagaaagact tttgacgaat tttcgaatga
     4621 aattttcatt ccatcttagt tggatttgat ttttcttttt ccactcttcc aatatttcta
     4681 caatttttcc gtacagaagt tgttcttttt ctgatagaag atagatcctt tccggtaaaa
     4741 atacttgtcc atctgcccac aacgtacgga caaattgtaa aaaggattgc ttgaaaataa
     4801 aattttctgc atcgtcgatt tcagcgatac taataatctg taaaatcaat tcattcagtg
     4861 ataggtcatt gagaacaaaa tttttataaa caacttcgaa atcttttctt aacagctcca
     4921 tgttctcatt ggtataattt ctactgttga acaaagaaaa aatataatag acatcttctt
     4981 cttgtaagtc tagaattcct ttttgctctt ccaatccttg acagactaat tgaaataacg
     5041 gcttcgtttc tgcgcttttc ttatcttctt ctttcagtcc aaggggaaat gcctttcttc
     5101 cgattgcgat ctctattcct cgtatgataa aggacttttc atcttccgaa tatcttcttt
     5161 taaaaaaatg agtctctaca taatcaatca actcgttaca agcttcttct acatacggat
     5221 ttttattttc atagccttcc caattgacat aacgtactaa agctagcaag atgttccgcg
     5281 agtcaaattc tgggataatg atttctttat ttttcaaata atcattttct ttaaaaaaat
     5341 ccaatacgat ttttttgatt gtatatactt tgctcaaaga aataaattca agatcagcca
     5401 gttctgtact cgtgaatata ccattaaaat aataacttaa aaactttaaa aaataagatt
     5461 gaccgtacag aaactgtgtg agctcagaaa gagcaatacc atgtttgaat acgagtttat
     5521 acttatgctt ctctcttact tgttctttta tacaatattc tagacactcg gctagacttt
     5581 ctagatcgtg aaggatcgtt ggtgtagtca cccttagaga aatagctgtt tgttcgatgt
     5641 ctatttcttg gaattcaaat aattgttcac acagatacac ttttctaaat atatttcgct
     5701 cgatgtaatt ttccagcatt cctgatacac gcctccagtt tttcttatga ttttatttaa
     5761 tcatatctgt aaatcgtatt tttgtttcct ttttatttag aaaagccctt tcaaaaacca
     5821 ataaaaaaag aaactgggac aaaagtcaaa caagctcaca atttcagcct cattgtaggt
     5881 agctttgagc cgtttggact tttaccccag cctctttcaa gaatggttat tcaaagatgt
     5941 cagcgtctag ttctttgact acatctttca acaattggat accagctgcg ctgttaccaa
     6001 atggatctag tgccgggctg aagacaccta tgccgtaacg atttggcaca gctgccatca
     6061 acccgccgcc tacaccgctt tttgcaggta caccaacatg gacggaaaac tctcctgatt
     6121 catcatacaa accagcagtt gtcataattg atttgacgat tgtagcactt tcttcagtaa
     6181 tgatttgttt gccattccaa ggagcaattc ctttatttgc cagcacagca gctaattttg
     6241 ctatgcctgt agcggtgaca ttgagggagc attgtctgaa ataagcatcg agtatctccg
     6301 gtacgtctcc ttcgatcatt tgattacctt tcatatagta agcaagagag cgattgatat
     6361 cacctgttct tgattcagaa tgatagattt cttcattcaa tgtgatatta ggatcatcac
     6421 agatctcttt cataaatgac aagattcgct caaatttttc atctgcatct ttaccggcaa
     6481 tcaacgaggt tgttgcaatc gctcctgcat taacaaaagg attcattgga tgtttgcgat
     6541 gattgatttc catattcatg atagaattga aggcaaaacc ggttggttcc gtgttgattg
     6601 tttgaaagac tttttctatt ccgttgtctt tgattgctaa taacaatacg ggaacttttg
     6661 acatgctttc gatggcaaag cgcacatcgg cgtctcctcc ctcaatcagt tgttttgtat
     6721 ccaaatcata aatcgccata cctaattgtt tgggatccac ttcagctaat gctggaatgt
     6781 aatccgccac ttgtcctgtt tgataatagt ggaaattttt tccaatcaat tgttctaatt
     6841 cttttacttc catttgaata accagctttc attttttatt tttctgcatg ctggaattta
     6901 tgacggcatg cgtagatcgt aaatggaata actaagacga taagataaga aacaatcaga
     6961 atcgtttcat aaatcccatg ctgtccagct gcgatactag caggtggaac aaatgagata
     7021 caaaaagcaa aaagtgaagt caaaagacct aaacaagcaa cgattacttt tacaactttt
     7081 ccgcctggaa cctgataggc tctttcaaga ctgtcatctt taaaaaccag cttcaaatag
     7141 cccgcgaaaa acataaagta caacatcagg tagattacaa cagtcaatga catcgctgca
     7201 agaaatgaaa gattattccc cccgccgctt aatgtaaaga cggctgccca gattgttgca
     7261 acgatcccat ttgcgatcat caaatgagca ggaacaccgt gtgtgttttc ttttgcaaaa
     7321 tattcaggaa gtaatccttc ttctgctgct acgtgaagtg cttttgaagg agaggttacc
     7381 caagaagcaa tttctccgat gacacctaaa caaacaagaa tcgcaagaat tttcgcaatc
     7441 catgcaagat ttggatcaat gtgtcttaat aggaattcta acgattgaat cacccctgta
     7501 ttaagagaca aaccatgttg aggtactgta gtagcgacgg tcaatccgcc aaaagtatct
     7561 aaaatgattg ccagaataac caatacaaac attgctaatg gataatcctt tttagggttt
     7621 tccatttcat ttgcatgact ggcagatgtt tctacgccca tatatgctaa aataaaggaa
     7681 acaaaaacaa ccagcgtagg caatttcgaa aaatcaggaa tcaatgtttt catagacagt
     7741 atcgtttgta gtggagcgcc tgaagcgaca taatggatcc ctaagccaaa caagactaat
     7801 gctggcaaca agataccagc tacaaaaccg atccgagcaa tcaatgaagt gtatttggtt
     7861 ccttttaact gtgagagtaa aatagcccag aaaataatca ataggattgc caatttcaga
     7921 tatttattgt tttgtatagc accaataccc gttgcatagg ataatgcacc agttaagaaa
     7981 tacagcatgg tcaagtagcc aactgttatc tcaaaccatt ggaaaaagat tgcggcaaaa
     8041 ccgaatcgtt ttcccaatgt gcgactgacc caagtataga caccgccttt ttcccatcct
     8101 ttcaccgttg ccatctctgc tgcgcacaat gccacaggga taaaccatag caaaccgcct
     8161 aataacaaga aaaatactaa gctgaagcct gaggtcgcga atgtgggata ttcatataat
     8221 gaaacgacca ttgagatggt catagccaaa aagccaaaga acgttaattt ttttgcagga
     8281 attttcgttt tgctttgttg catgaatctt cctcttttct atttgaattt gtttcaattg
     8341 agtttgttgc caaagaagaa aaagtccaat ccttcttctt gttcttcttt tgtagtagtg
     8401 atcgttgtaa agtgcatcgc cgattttaaa atcactgttg tcagcacata ggcatacacg
     8461 gcagtaatga tgacaccaat cacttgaatc cctaagaagt accagctgct tggattcgtt
     8521 agtaaatggc tgttagatgc aaagatccct atcagaatcg tacctgtgaa tccgcccatg
     8581 ccatgaacac cccacacatc aagtgcatca tcccattgtt tctttttacg aaaatcaaca
     8641 gcagcatgac agacgatacc cgcaatgatc ccaatcagca tggcactttt tgcttctaca
     8701 taaccggcac aaggcgtaat ggctgccaat ccagcaacgg atcctgttag tacgtcgaca
     8761 aaatcaaagc catttccttt tacttttgca atgatcagcc aagtaatcat cgcaaatgca
     8821 agagcgatga atgttgaacc aaatgcattg actgcttgtt catctgctcg aagtgcacca
     8881 ccggaattaa aaccgaacca gccaaaccaa agcagtcctg taccgatagc agctgccata
     8941 agactgctat gctgacttct ttctcctaaa ggaatctttc tttgaccaag tgtgaggata
     9001 catgctaaag agccgaatcc agctgtcgtg tggatgactg ctccaccagc gaaatctcta
     9061 aatcccatct gatccaaaaa tccgccaccc catatccagt ggcaaacagg gaaataaatc
     9121 agcagattcc aaaaaataac aagtataata tagcctttta aattcaatct ccctgcaaaa
     9181 gctcctgtca tcaaaggaac ggtaatcacg ataaacatca gttggtaaag aaagaacatt
     9241 aaaaatggaa tcgttgcacc atgaaacatg ttggggaagc ttgtaacatg tctcatcaaa
     9301 aagaaatctg ccgggtttcc aataacaccg ccgatatccc gtccaaaggc caatccgaat
     9361 ccaccaaata cccacataat cgttgttatc ccaacggaga gaaaagattg agacatgatc
     9421 gttagtatat tattcttccg tgctaatccg ccgtaaaaaa aagctagacc aggagtcatc
     9481 aggcaaacca ttgctgtaca taaaatcatg aatgctgtat ctcctgtatt gataggtaat
     9541 gtattcattt ttttcgcctc ctttctacga ttacgagtat aaaagaaggt gtagttgttt
     9601 ttttcttctg tttttttgaa ttaatacttg catttataaa agacagacaa ttaataaaag
     9661 cttgatgtgt tgtgctcttt ttcgtttttc acacaaataa agacaaaaaa taaacggtct
     9721 tctttttata gaaaaccgtt tattttcgtc tttttttatt tttaaagata ttttttcata
     9781 ag
//
LOCUS       DACDSY010000107         9674 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDSY010000107 DACDSY010000000
VERSION     DACDSY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.108"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            231..1280
                     /locus_tag="IWK44_002145"
     CDS             231..1280
                     /locus_tag="IWK44_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9550640.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            1277..2158
                     /locus_tag="IWK44_002146"
     CDS             1277..2158
                     /locus_tag="IWK44_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9550641.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            2161..2931
                     /locus_tag="IWK44_002147"
     CDS             2161..2931
                     /locus_tag="IWK44_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9550642.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            3210..4064
                     /gene="aroE"
                     /locus_tag="IWK44_002148"
     CDS             3210..4064
                     /gene="aroE"
                     /locus_tag="IWK44_002148"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP9550643.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            4242..5405
                     /locus_tag="IWK44_002149"
     CDS             4242..5405
                     /locus_tag="IWK44_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9550644.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            5522..5932
                     /locus_tag="IWK44_002150"
     CDS             5522..5932
                     /locus_tag="IWK44_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9550645.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6106..8334
                     /gene="topB"
                     /locus_tag="IWK44_002151"
     CDS             6106..8334
                     /gene="topB"
                     /locus_tag="IWK44_002151"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9550646.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            8472..8789
                     /locus_tag="IWK44_002152"
     CDS             8472..8789
                     /locus_tag="IWK44_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550647.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            8949..9185
                     /locus_tag="IWK44_002153"
     CDS             8949..9185
                     /locus_tag="IWK44_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9550648.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            9182..9580
                     /locus_tag="IWK44_002154"
     CDS             9182..9580
                     /locus_tag="IWK44_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9550649.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3352 a         1640 c         2053 g         2629 t
ORIGIN      
        1 gcgtagataa gttgtattaa tggtgagact ttcccataga tacaactttt tagagtgcat
       61 ttctaaagct tgtatctatg ctagacatga acattgactg ccgcatagat tagaatctat
      121 gcggtttttt gtgtaaagac aatgaccgct ttggccattg tctttttttg tagactggat
      181 acttgcctga ttataataaa taaaaagatt gactttgcag gaggaaaaaa atgaaaaaca
      241 aacgattaat aacagttgta gtaattattt tagtttattt agcaggtgca tttatttatg
      301 gaaaagcctc aggcgaaaaa gaaagtactt cgacgaacca gacaaaaaaa acaacagttg
      361 ttggggtatt gcaatacgtg agccatccgg cattagatga gatttatcga ggaatcaaag
      421 atggtctgaa agaagaagga ttaaaagaag gagaaaatct tgagatcaaa tttcaaaatg
      481 gacaagccga tcagagcaag ctagccacaa tgagtcaaca gctagtacaa tctgatccag
      541 atgccttgat tggaatcgca acacctgccg ctcaatcatt agcaaacacg acttcatcga
      601 tcccagttgt gttaggtgct gtaacggatc ctgtgggggc tggattagtc gcttctttag
      661 agaaacctgg tggtaatgta acaggtgttt ctgatcagcc gcctgttact tcacaaatca
      721 agcttgcttc tgatttgttg ccaaatgcaa aaaaagtcgg aatcttgtat gcttcttcag
      781 aggataattc aaaataccaa gtagcacaag cagaagaagc tgcaaaaaaa gaagggttag
      841 aatcagtaaa atatgcggtg ccttccacta atgagattgc acaaacggtt caagtgatga
      901 gccgacaagt cgattttatc tatgttccat tagataatac cattgccaat gcgatgcaaa
      961 cagtggtaaa agaagcaaac aaagcaaata tccccgtcat tccatctgtg gatacaatgg
     1021 tggaacaagg tggactagca acaatcggga tcaatcaata tcaattaggg cttcaaagcg
     1081 gtaagatggc ggcagaactt gcgagtggaa aagaaaaacc ggaaacgacg cctgtttata
     1141 catttgatca aggggataca gtgatcaatc aatctcaagc tgatcgctta ggaatcattg
     1201 ttccccaatc gatgaaggaa aaagccaaaa tcattacaga tgactcgcag caagaaacat
     1261 ctaaggagga tgacaaatga tcgtatcagc tatttcacaa ggcatgcttt gggcaatatt
     1321 aggattaggg attttcatga cttatcggat cttagacttt cctgatatga cgacagaagg
     1381 gtccttccca ttaggtgctg ctgtctgtgt gacagctatt actcatgggg tagcgccgat
     1441 tgtagctaca ttattaggcg taggtgctgg catgatagca ggactcgtta cggggttact
     1501 ttatacaaaa ggaaagatac cggttatttt agcagggatt ttagtgatgt caggattgaa
     1561 ctcagttatt ttgtttgtga tgcgaacacc aaatctttct ttattgaatc atccgattct
     1621 acaagacagc ttccatcgct ttgggttacc tgattacttt gacacagtcc ttcttggctt
     1681 catctctttg ggcatcttga ttgctgtact gctattcttc ttcaatacag atttgggtca
     1741 aggctatatt gcgacaggag acaacgaaac gatggctcgg tcgttaggga taaaaaccga
     1801 tcgtatgaaa attttaggtt taactttgtc gaacggtgtc attgcattat ccggtgcgct
     1861 gattgcccag aacgacggat atgcagatgt aaataaaggg attggcgtaa tcgtcatcgg
     1921 tcttgcttcc atcatcatcg gtgaagtgat cttccaagaa ttgacgctag cagaaagatt
     1981 gattgctatt gttgtcggca gtatcattta tcagctattg attttagtag taatcaaact
     2041 agggtttgac acgacttatc tgaagttgtt ctccgctatc atcttagcta tttgtctgat
     2101 gatcccgcaa ttgaagaaag cactgaactt aaaaacgggt tttgagaagg aggcatagta
     2161 atgactgata tattgacaat caaaaatggc agaaaagtcg taaacaatgg aatgaatgaa
     2221 gagaaagtac tattggatga aattaacctt acaatcagag aaggggactt catcactgtc
     2281 cttggcggga atggtgctgg aaaaagcaca ctgttcaata cgatcgctgg tacgcttcaa
     2341 ctcagtcaag gatcgatctc ttttaaagat cagctgatca caaaagattc agaagaaaaa
     2401 agagcacgat tcctttctcg agttttccaa gaccccaaga tgggaacggc tcctcggatg
     2461 acagtagcag aaaatttgct acttgcacaa aaaagaggtc aaaaacggaa attccgttta
     2521 cgtaacttga aaaatcaaaa agaagatttt ttcagactat gtcaagaagt cggtaatggc
     2581 ctagagcaac acttggatac tccagcgggt gaattatctg gtggtcaacg ccaagcatta
     2641 agcttgctga tggcgacgat cacagctcct gagttactat tattagatga acatactgca
     2701 gctttagatc ctaaaacagc taaagcatta atgcgattaa caaatcagcg gatcaatgaa
     2761 caaaaattga cttgtctcat gatcacgcat cgaatggaag acgccttgaa atacggaaat
     2821 cgtctgatcg tactccaaaa agggaaaatc gttcaagata tttctgaaaa agaaaagcaa
     2881 catttgacaa tgggcgacct tttgcagttc tttgaagaag tagtggattg atttcgacag
     2941 aaaaattcct ttcagtagaa ggacttgaaa actcattgac atcttccttt gattcatcct
     3001 atacttaaaa caataaacaa ttaaacatat ttgggtgttt tgtaggatca cgtagtcaat
     3061 gattttaaca attaaataga atattctttg ctctgacaag taaacgatga gaaaagaatt
     3121 tgctactttg ccacctagac gtttcgtact ttttcgtatc aaacgctaag tggctttttt
     3181 tattgaagta agaacaaagg aggaataaga tgatttctgg acaaaccaaa ttagctgggc
     3241 tctttgctga tccggcttct cacagtttat ccccattgat gcacaatact gcttttgaag
     3301 caaacaacat cgatgcggtg tatttagctt tccaagtaaa tccaatgaat ttgaagcaag
     3361 cagtcgaaag tattcgaact tttgatatgt taggtgtaaa tctttcgatg ccgaataaaa
     3421 cagcggttat tccttatctg gatgaggtaa gccaggaagc gcaattgatc ggagcagtga
     3481 atacgatcgt gcacgaaaag ggtcgtttga ttggctataa taccgatggt gcgggcttca
     3541 tgcaatccgt gcaagaagct gggatttcga tcaaaaagaa aaaaatgact ctactgggag
     3601 ctggaggtgc agcaaaggca gtcactgttc aagctgcgtt agacggcgct gaggaaatcg
     3661 tagtttataa acgaaacaac caaacattcg ctcaagtaaa agaaacattc gaacaactca
     3721 gctatcgaac ttcttgtaag ataaaagttc gtgactttgc cgatacgcat caattaagaa
     3781 aagacttgaa agaaagctgt cttctagtca atgcaacaga tatcgggatg ggaaagaaaa
     3841 tcaatcagtc accgctctta gatataagca tgatgcatga gaatctagcg gtttttgatt
     3901 tgatttatgc acctagagaa acacgtttat taaaagaagc aaaaaaagta ggagcaaaag
     3961 gatacaatgg acttggcatg ttgatctacc aaggagcaat cgcgtttgaa ttatggacaa
     4021 aggaaaagat gccgatccat gtgattgaaa agctgtttga ttaaaaaagg ataatagata
     4081 aagatctttt attctcgcat aaacggtctc aagaaaagta atcctttatg tatcgaagca
     4141 tttatttcgt tattttttat taaattatca aaaaatgata ggcttttgtc gattggttat
     4201 cgtatccttt atttatcagt aagaaacgag gaggaaccac aatgagtcgg atggatcgct
     4261 ataaggacat ccataagaag gcgaaacctc tgaagcggga tacggggagt atttttgcta
     4321 gaagagaacg aaaacgaacg cgtatggaaa atgaggaaga tgctacccgt atttatgaaa
     4381 gagagcagca ttcatttgaa catttgaacc aatacgagca acagatatac agagatgcac
     4441 caagagcaga agaacaacaa aaaaataaaa aatttttcaa tcggcaaaag aagaacaaac
     4501 cgttaaaacc tcgaagaaag cgatcgtgga aaaaaatcat tctatcgatt ttgcttgttt
     4561 tgatccttta ttcaggtatc tcatttttta ttggtaaata tgtagctgaa catgacacaa
     4621 gcttagaggc agcgacttca gaaacctttc atgggcaaaa agcgacgaat ggtgcgcata
     4681 atattttgat ccttggaagt gatacacgcg gagaagatgc cgggcgagcc gatacgatca
     4741 tggtcttaca gttggatggt ccttcccata agccgaaact gatttctttt atgcgtgaca
     4801 gcttcgttac gatcccaggg gtaggacaaa ataagatcaa ttcagcttat gcatacggag
     4861 gagccgatct agtgcgtcaa actttagtag aaaattttgg aatcgattgt caatactatg
     4921 cgaaagtcga tttcaaatcg tttgaaaaag tgatcgatgc cttattcatg aacggagtga
     4981 agatcgatgc agaaaaagat ctgaatctgg atggtgtaga tattaaaaaa ggggtccaga
     5041 agatggatgg acatgttctt ttgcagtacg cacgtttccg aatggatgaa caaggagact
     5101 ttggccgtgt cagacgccaa cagcaagtaa tgaacgctat tttcagccaa ttgaagaatc
     5161 cattgaatct gatccgtgca ccttatgcag ctggtaaagc tttaggatat acatccacag
     5221 atgtcactgc ttggttcttg acgaaaaacc tcttttcgat tgcacgagga gctggtggca
     5281 ttgatcgaat aagtgtgcca gtagacggtt cgtggaatta tgggcaaagt gattatgctg
     5341 gcagtattct tgaaattgat aatcaaatga atcaatctgc tattacagat tttttgaata
     5401 aatgatcata aaaaagatca agaagaagct gcgcaaggct tttgtcatga atcaatcaaa
     5461 cgattcactc gtaaaaaacg ctttcttatg gtagatttaa ccaagagagg agctgtttac
     5521 attggatatc tatctgaact tcaaaaatga gtcgcgtgaa gccatcgctt tttatgaaaa
     5581 agtttttcag actgaagcat caggtatcac cacatacggt gaaatgcctg gaatagaaca
     5641 aatgaacttg ccagaagaag caaaaaatct aatcacgaat gcacggctga acatttatga
     5701 tacattagtt atgtttgcag atacgccaga aggaattggt cctgaattag ttgtgggaaa
     5761 taatgtttct ttagtcatcc agacaagaaa tgaagaagaa acgaagcgcc tatttgaaag
     5821 cttagctgag ggtggtaaga ttgagatgcc actggatgct gttccgtggg ctcagttgta
     5881 tgggatggta actgatcgat ttggcatcaa ctggcagatt aaccaagagt gacagctaaa
     5941 gatgaatatt ttctggaaac atcaagcagc aaacggatca tcgtttgttt gcttgatgtt
     6001 tttgtgtcta tttcgactat acggtatctt ttttttattg ccagatgcga aaatatgttc
     6061 cataatagag aagtccgcaa aaaattaagt aacggaggtc agaagatgaa gacggtgata
     6121 ctagctgaaa aaccttcaca agcaaaagcc tatgccgaaa ccttccaaca gcaaaagcgg
     6181 catgaagggt attatgaaat cagcgatcca ttgtttgctg gtgaagtaac catcacttat
     6241 ggttttggac atttggtaga tatggttgct ccaggagctt atgaagagcg ttgggcaaaa
     6301 tggtctttag aaaatttgcc tattttccca gaaaccttcc gttacgaagt tccaatggac
     6361 aaacgtgcac aattttcgat cgtaaaacga gaattgcagt cagccgatac catcattatt
     6421 gcaacagatg gtgatcgaga aggtgaagcc atcgcgtggt cgattattcg acaagcaaac
     6481 gcattttcta aggaaaagaa ctatttacgt ttatggatca attctcttga aaaagaagca
     6541 atctatgaag gttttaagaa tctccaacct ggaaagaact attttccaaa atataaagaa
     6601 gcgcaagcaa ggcagaacgc tgattggctg attggaatga atggaagtcc attatatagc
     6661 ttgctacttc aacgaaaagg gattgatggc agtttttctc ttggtcgtgt acaaacaccg
     6721 acactctata tgatttatca gctgcaagaa gaaatcaaac acttcaaaaa agaaacgtac
     6781 tttgaaggag aaggtttaat tacgacaaac aaagggcagt tttctggaaa aatcgtacca
     6841 aaaaaagcat ttaaaaccca agaagagtta acgaaggaaa tagaaaaact tggagcgcat
     6901 ttaggtaagc aaaacggacg gattcttgaa gttacgaaaa aggaaaagag gttgcatagt
     6961 ccacgtttat tttcgctatc tagtttgcag acaaagatga atcagctaat gaaagcaagt
     7021 gccaaagcta cacttgatgc tgctcaagga ttgtacgaaa gcaagtttct aagctatcca
     7081 agaacggatt ccttttacat tacagaaaat gagcatcagt atctggttaa acatctgcaa
     7141 aattataaaa aatttttagg aatagaagaa gtacagacaa ctgagatcaa agcaaagaaa
     7201 cgatatgtcg atgcgaaaaa agttcaagaa catcatgcaa ttattccgac aaagacgatt
     7261 ccgactcctg cacgctttgc gaagctttcc aaattacagc aagcaatcta tttgcaagtt
     7321 ttgcgcacta ctgtggctat gtttgctgca gattatatct acgaagagac gaccgtaatg
     7381 acgggcgtac agcaattaaa gctaaaaagc atcggaaaga tcccattaca gcaaggttgg
     7441 caggaaatct tacgcatcaa gtcaaaaaag aaagaagctc agactcttcc tcctatcgta
     7501 gaaaatgaag aagcagaagt agaactgaaa agtatggaga aagaaacaca acctccaaaa
     7561 ccctataccg aaggaacgtt gatcacagca atgaaaactg cgggaaaaac gctggataat
     7621 gatgaagcgc aagaaattct caaagaagta gaaggaatag ggacagaagc tacacgagcc
     7681 aatatcattg aaaatcttaa gcagaagcat tacatcgaag tcaataaaaa cgaaataact
     7741 gttacgccaa aaggaatcac tctttgtaag gcagtagcca atgaaccatt actgacgagc
     7801 gcagaaatga ctgcgcgctg ggaagggtac ttgagaaaaa ttggtaaaca agaggggaca
     7861 ccatctgttt ttttagagaa tatcaaaaaa ttcatcctac atttattggc agaagttcct
     7921 aatcaaatcg acaaggtaga cttttcagat gagattagcg ggacacgtca gataaaagcg
     7981 atggagaaga aaaacgatca attaggaaga tgtccaaaat gtaaaaaggg aatagtcatg
     8041 ctttatccta aaatcgctac atgcttgaat ccggaatgcg acttcaaatt gtggccaaca
     8101 gtagctaaaa agaagctgac taaaacgaat ctcagagatt tgctttcaaa aggaaagaca
     8161 agtaaagttg tcaaaggttt tactggtaaa aaagggaaat tcgatgcagt tttagtatta
     8221 aaagaagata tgacaatcgg tttttctttt ccatggatcg aaaacagtgg aacaaaagca
     8281 gttgatggaa acaaagaaac atcaagaaaa aataatgaaa acgaagtaga gtaaatgaaa
     8341 acaatgaaat agcggaatat ttattccgaa aagtgagaat ataaatacca ataaaactaa
     8401 aaaatatcag tgaatcaagc aaaaacgttg gggtaggctg attttatata gatagcaagg
     8461 agttttttta tttgaaaaaa gtgcattcat ttttaaatta tcttatggga gcaatcacga
     8521 ttgtggctgt gtaccaagca tttctaaagt atcctgatta cgggacgaag ttgttgtcat
     8581 tgacgcacaa tcaaacagca tattcaccaa cgttgatttt atcgactttg actgcaggag
     8641 ctaaaagcgc tacttatttt tttcataaaa cgacaagcat gctaaagcga aagaaacagc
     8701 gaaaacaatt gttggtggtg gtagcggcta tgatcattat ttattttcta ccagttatta
     8761 cacaagtatt tcatcaatat tttggttaag aatacgagag tttgtgacaa aagtcatgtc
     8821 acaaactttt ttctttgtca ttttttcgta agaaaaagca aaataaaagt atatacattg
     8881 attaaaagta tatacttgga tataatgtac ttactataga atgagaatgg tatagagggt
     8941 ggcgaaatat gaaaaaaatg aaaaagtata aaattagaaa atctggcaat agcgatgtaa
     9001 caactatccc accagaagta aaagaattca tgggtgtgca gacaggtgac gcaatcagtt
     9061 atgtttttca atctgatggt tctgtacgaa tgatcaaagc acaagaagaa cctgatattg
     9121 attcattagt tgattcgatc atgaatcaat atgaagacgc gttaaaagat ttggttgatt
     9181 tatgaggtat ctttcgacta aagaaattat caaaatcaac gcgaaagtta ttttacgtca
     9241 ctcgccaggc gaaatgatcg gtgtaaaaga tgcaaatgca ttagatatgg ccgtcaaaca
     9301 gccttcacaa gcagtattca atcaagaact ctatcctgaa atctatgaaa aagcggcgat
     9361 tttagcaatt aatttagcta aaaaacatcc ttttcataat ggcaataaac gaactgctct
     9421 tgttgccatg ttgttgtttc ttcaattgaa taattgtcca gttgcttttt ctcgtcaaga
     9481 agctgttgat tttatcataa tgattaccac tagttcattg gatttcgatt cactaaagtc
     9541 caagataacc agttatttac gacaaaccgc aataaaatga acgaattaaa aactttattt
     9601 ctcaattaga tcagcatgtc attaaggaat atgctgattt ttttatgttt aatcatacaa
     9661 aatagttttt tcat
//
LOCUS       DACDSY010000108         9630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDSY010000108 DACDSY010000000
VERSION     DACDSY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.109"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(14..676)
                     /locus_tag="IWK44_002155"
     CDS             complement(14..676)
                     /locus_tag="IWK44_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9550650.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(689..2191)
                     /locus_tag="IWK44_002156"
     CDS             complement(689..2191)
                     /locus_tag="IWK44_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9550651.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            2739..4418
                     /locus_tag="IWK44_002157"
     CDS             2739..4418
                     /locus_tag="IWK44_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9550652.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            4545..5024
                     /locus_tag="IWK44_002158"
     CDS             4545..5024
                     /locus_tag="IWK44_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP9550653.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            5243..5923
                     /locus_tag="IWK44_002159"
     CDS             5243..5923
                     /locus_tag="IWK44_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP9550654.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            5931..7262
                     /locus_tag="IWK44_002160"
     CDS             5931..7262
                     /locus_tag="IWK44_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9550655.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            7277..8239
                     /locus_tag="IWK44_002161"
     CDS             7277..8239
                     /locus_tag="IWK44_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP9550656.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            8350..8688
                     /locus_tag="IWK44_002162"
     CDS             8350..8688
                     /locus_tag="IWK44_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP9550657.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            8767..9474
                     /locus_tag="IWK44_002163"
     CDS             8767..9474
                     /locus_tag="IWK44_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP9550658.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         3058 a         1735 c         2071 g         2766 t
ORIGIN      
        1 attatcctgt tttttaaact tcgatttttt gaaatttttt attttttagt tcaaagactt
       61 catcacagct ttttgctact tcaatctcat gagtcacgat gataatacag cgattttgtt
      121 catgggcgat ttcttggaaa agtttgacga tatcttgtga agttgtttca tctagatttc
      181 cagtaggctc atccgctacg attagttgat gttcacagca gatagcacga acgatagcga
      241 ctcgctgctg ctgacctccc gaaagtttgg tcacctgttt ttttgctaat atttcatcaa
      301 ttcctacacg agccagtcga tctaaagcaa cttgtcgata attctcttgc ttggtcttag
      361 tgatttccat tgctgctatg acgttgtcta gagccgataa ataaggaagc aagttgtaag
      421 cttggaaaat aatcgagaca tcatttttac gataacttct taacccgatt ttgttcagtg
      481 aacgatcttg atagtatacc tttccttctt ttggtgcatc tagtccagaa ataagcgaaa
      541 gaaaagtcgt ttttccagaa ccacttgctc ctaaaatagc gtacattttt ccagcttcga
      601 aattcagttc tacatcttta taaagcgcat cttgcggatc ttgataccaa aatcccaatt
      661 gttctgtccg taacatcaaa ttcctccttt agtttaatag gatttttctt gggtttaatc
      721 ttaatatccc tatagaagct aaaaagacag aaaacagact gattccaaat gctactccgg
      781 ctaatagtcc caattgtgca ggtttgacat tgatttccaa ttcgctgact tgatcagaaa
      841 cagcaaatgg attattcgtg gttccttgtt gaccatcgcg ttgaccgcct tgttcttgtc
      901 tgttacctgg catttgtcca ccgccaggag cttgctggtt ttgtgtcgtg gtttctgtct
      961 gttggtcaag tagctgctgt cccacggcat tcgcgacgac gtttccgcta agcgaagcaa
     1021 ctcctaatgc aagaatcata caaatagcca cttcggtaaa caattggaga atgattttac
     1081 ttctggattc tcctaaagat agcaagacgc ctaactcatg ccgacgctcc cgaatggtaa
     1141 tcataatgat cagagtaagt atgataatac cggctacagc cactagtaaa acaacatttt
     1201 tagaaaaact tgccacgttg tttaaaggtt ccaacattga ctgatacatg gaatcatttg
     1261 attgtaagct aaatgtatct gtgtcgatca gcttctcagc ttctttgaga aactcatcca
     1321 ttttatctgg atcagataga gtgtaaatag cagaatctat cgtgttatcg tcagatgtcc
     1381 catttagttc atttgcaaaa gtgtaagaag tgtaaagtgt attcgacgga ttcatgaagt
     1441 tgaatttcat acccatactg ccgcttgttt cgctggattc ataaattcct ttgatcgtca
     1501 tttcataagt ggtatcttca tcttcagtac tcgtgattgt gaaagtatct ccgactgcca
     1561 ggtcatttgt ttctgctaga gtagaatcaa tgactacatt gtttgtgcct ttgtcgctgt
     1621 catctattcc ttcaccatcc gtgatttttg ctgttccatc agaaaaatta ctattttgtg
     1681 cagttgctga cacaccactg atttgaaagt ctgcttgagt catttgcgga ttcccgccca
     1741 ttcctccagc aggttcaccg cctgcatcag tagtttctga actgtcttca cttgaatcag
     1801 aactagtgat ggcagttatt ccatcttttg ccatagctga ggcggaactt tcaaaggaat
     1861 aactctttac attatctaat tcagctattt tttccgcgtc cgttaaggaa actggtgtaa
     1921 gagaaaaact gcctggatct ggacgttctt cagtggtaga accgcttgaa gaagtttgtt
     1981 cctgtttttt aaatgaagct tctcgattgg cacttaaagt cactgttgct ccaacttctt
     2041 tttgtgcttg attcgctgct tcttctgcag cgcttcgaat cgtgaggcca gctagcacaa
     2101 aaataagaat ggctgtgaac acggcaaaca ataacaatga acgtccccat tttacttttg
     2161 tacttttcca tgcacgtttc ataaaattca tattttgcat ccctcctagt agatgcattt
     2221 gataatagac aacctatctt aaagctagct taaatcttat gctgtttgca caccatcaac
     2281 atgaggttct gtatatataa aaagtcacta tagttattgc aaaaaatgtg gtaaaatgaa
     2341 aaaagtatgt ttcaaaccga cgaagaagaa atgaaagagg aatagcaaaa gaaatcctag
     2401 atgctacaga gggattttta cgtgcatagt tgttctaaca attgctgata ctagcgattt
     2461 agtttatttg ttgtataata atggggaaca gctgaatact tatataagta ttctggctat
     2521 agcgtttcgc aaaagatcca ttttgttaat gaagtatatc caaaataaaa tgcccatcca
     2581 actgtcagaa cgcggatgtg ctggtaattt tcctgctgat tctccataag ttgagaaata
     2641 gtgattttgc atatgcttca ttggaatggc tcatatattt agataaagaa gaaaatcgac
     2701 atctttaaac ttggatgtca taagaagagg tgaagtttgt gagtaccatt aaaattatcc
     2761 cattaggcgg cgttcgtgaa aatggaaaaa acatgtatgt cgcagaagtg ggcgaggata
     2821 tttttatttt agattgtggg ctaaaatatc ctgaaaatga gttgttgggg atcgacgtcg
     2881 taatccctga ttttacgtat ttagaagaaa acattgatcg tgtagctggt gtctttttaa
     2941 cacatggaca tgcagatgcg atcggggcac ttccttactt gttgtcaaaa atttctgtac
     3001 cagtattcgg gacagagttg acgatcgagt tagcaaaatt gaatgtcagt cgtaatgaag
     3061 cagccaaagg atttaaggat ttccatgtgg tagatgagca tacagaaata gactttggtg
     3121 aaacagtagt gagctttttc cgtaccactc atacgatacc agattcagtg gggatcaatc
     3181 tgaaaacaaa agaaggtagt atcgtttata cgggagattt taaatttgac caaacggcta
     3241 tcccaatgta tcaaacagat tatgcacgat tggcggaaat cggcaaagaa ggagtcttag
     3301 ccttactaag tgaatcggca aatgcagaaa atcctcagcc tgtagcttct gaagcccaaa
     3361 tagcagatga agtatttgat acgatcaaat attgggaagg gcgtatcatc gtcgcttgtg
     3421 tagcaagtaa tttgcaacga gtgcaacaag tattggatgc agcagaaaaa gcacaacgaa
     3481 aagttgtctt gactggtcaa gatttcgaac gaattattcg cacagcgatg cgtttagaaa
     3541 aacttcaatt accaagtgaa gatctgcttg ttacgttgaa agacatgaaa aactatgatc
     3601 cagaagaact gatcatttta gaaactggcc gaatgggtga acccatcaag tctctgcaaa
     3661 aaatggcaaa cggtacacac cgtaatgtca agattgaaga aggagatttg gtttatatca
     3721 ctactacgcc aagcatcgcg atggaaacca tcgtggcgaa aacggaagat atcatttatc
     3781 gagctggtgg aacagtgaag ttgatctctg agaatatgcg cgtttctggc catgccaatc
     3841 ctaatgactt gcaattaatg atcaatttga tgaaaccaac atattttgtg ccagttcaag
     3901 gagagtatcg tgaattagct gctcatgcag atttggctca tgcagtaggg atgccataca
     3961 aaaatatcta tattactgga cgcggagacg tgctggaata taagaacaag cgtatgagtg
     4021 ttgcaggaac tacaagtgca gaaaatgtaa tgattgatgg attaggcata ggtgatatcg
     4081 gaaacattgt tttacgtgat cgaaaaatct tgtcagaaga cgggattttc gtagcggttg
     4141 tcactatcag tcggcgagaa aagaaaatca tttcaaaacc tcaaattact tcacggggat
     4201 tcgtttacgt aaaagctagc cgtgatctga tgaaagaaag cagcaatatg attgaaacaa
     4261 tcgttgagaa acatttagaa agcaatgatt ttgagtggag caaattaaag caagacatcc
     4321 gagatcaatt aggccgttac ttatttgaac aaacaaaacg ccgcccagtg atcttgccag
     4381 tcattatgga agctactcaa cgtcgaaata aaaaataaac aaaaagagta agatgaacct
     4441 ctctatagag tgctcgtctt gctctttttt tgcgaaaaac aaataatttt tgaatacagc
     4501 atccctcgct ttataataaa cgatagaata aagggagtga atcaatgatg agtcaaacaa
     4561 aagtcgaacg ttatttagaa gaacaaaaga tcagctatca accacttgat tttagtcatg
     4621 tggtagaaga atctttcaca gaagaactac aaaaaagagg gattgatcca gctttgatat
     4681 gtaaaactct cgtagtgaaa ggaaacaaaa ctggggtgat catcgcattg attcctttga
     4741 acgatcactt agattacaaa aaaacgagac aagtgacgaa agatcgtaaa gttggttttc
     4801 ctggaatgga ttttgtatta atgcatacag gatatcccca cggagcaaac acccctattg
     4861 gaataaaact cgcacatcct gattattgtt ttttagcaga cagttcttta aaagaaaaag
     4921 aagaagtaat tgtgtcaagc ggtgaaatcg ggagaagcct tcggataaaa gtagccgatt
     4981 tagaaaagat cgtccatcct atttacgctg atttaattag ttaagggacg agaagagaag
     5041 ctaagcagag cagagacctc aaacttgaga ttatatctgc ttggtacttg gtgcatcctg
     5101 tattatctgt tacaattgct ttgtacatac tgtactatca atccaatgaa aatatattta
     5161 gcagctatat tttttcattg atgtgcaagt gatgaaatcg tttttaataa aaaagataca
     5221 gagaaaacgc gaggtgtaaa atatgaaaat tacgtatcat ggacattcct gtttaacaat
     5281 tgaaatggaa aatggccaac gcgtgatgat tgacccgttt attacaggaa atccattgac
     5341 agacttaact gcagaagaag taaagacaga ttggttgctg attactcatg gtcatagtga
     5401 ccacatagga gatatgattc ctattgcaaa agaaaatcaa gccacaatta tcagtattgt
     5461 tgagattgct gattatgcga acagcagagg agtagattca ttcggtatga atatcggtgg
     5521 aaaacatgcg tttccattcg gcgtggtaaa attcgtacat gcacagcata gttcaagcta
     5581 tgaagtagac ggtatcgttc agtatatggg cgagcctagc ggaattatta ttcaagcaga
     5641 aggaaaaacg atttatcatg caggggatac tgcttatttc agtgatctag gattgcttgc
     5701 agaagaattc gatattgatg tcgctttctt gccgattggc gataactata cgatggggcc
     5761 agaagatgca gcaaaggctg cgcaaacaat tcgtgccaag aaagtagttc caatccatta
     5821 caatactttc cctgtcattg aacaagatcc cgaatctttt gtagagttac tggaagatag
     5881 tgaaggaaaa ataatgagcg tctcggaaac gctggaagtg taggcagaaa atggcgacaa
     5941 aacatgattt gattttagaa tatattgaaa gtttacctgt tggcaatcga atttctgtaa
     6001 gaagcatcgc aaaagaattg aatgtcagtg aaggaacagc gtaccgtgca atcaaagatg
     6061 cagaaaatgt cggactagtt tcaacaattc aacgagtagg gaccatccgt atcgaacgca
     6121 aattaaaaaa acatattgaa cgtttgacat ttggcgaggt cgtcaggatc attgaagggg
     6181 acgttttagg cggggcggca ggattagaca aggtcttgaa taaattcgtc atcggcgcga
     6241 tgactaaaca tgccatggaa cgctatatta cgccaggatc actaatgatc gtcggcaacc
     6301 gggcggaagt ccaaaaatta gcattggaag atggcgccgc agtattgatc acaggaggat
     6361 tcaatacgac tgctgagatt gcaaagctag ctgatgatct tgaaatgccg atcttacgca
     6421 cctcttatga tacatttaca gtcgcaacca tgatcaaccg agcgttaagc gaccaattga
     6481 tcaaaaaaga tatcatgctt gtcagtgata tctatatgcc agctgaaaag actcattatt
     6541 tgcagcagac agatacgatt tctgattatc agcgtttgtc agaagaaacg cagcattcgc
     6601 gttttccagt cgtcaaccgc catcatcgtt tgatggggat tgttacggca aaagatgttt
     6661 tagggaaatc gccaaaccag atcatcgatc gggtgatgac gaaagaacca atcagtgtaa
     6721 agaaaacaat gagtattgcc tctgttagcc atcaaatgat ctgggatggg ctagaagtca
     6781 tgccggtcgt ttccgatgac ttgacattgg aaggcttgat tacccggcaa gatgtaatga
     6841 aagcgatgca gcttgtccag cgacaaccgc agattgcaga tacgatttcg gatcagatat
     6901 ctggaaatat ccatacgatt gatactgacc gtgaaggaaa tcgtttggaa aaccctaagt
     6961 tcaaattcgt agtagcacca caaatggtca atggcgttgg aaccatttct tttggcgtac
     7021 ttagcgaaat catttctgat gtcgcacaaa aaacaatggt gatgaaccaa aaacgaaata
     7081 tcttgattga acaagtgaac ctacattatt tgcggctgat ccaattggaa agcgaattag
     7141 acattcgtcc acgtgtattg gaaatcggca gacgttccgc taaactggat atcgaagtgt
     7201 ttattgaaaa caccatcgtt tccaaagcaa tcgttgtttg tcaagtaatg gaacgttctt
     7261 agaaagcagg agtgaaatga cagtacagca agaaatagta gatacaataa aaaaatacga
     7321 taaaatcatt atccacaggc atttgcgccc tgatcctgat gcattaggat cacaagtagg
     7381 attagccgaa ctattacggg aaagtttccc agaaaaagaa atacgtcaag taggtgaaac
     7441 gattgaaggg cttcgctttt tagcagaaat gcaagaagtt gaagatgtct tctaccaagg
     7501 agcgctagtc atcgtgactg atacagctaa ttcacccaga atcagtgatc aaagatatca
     7561 gttggcagaa aaactgatca aaatcgacca tcatcccaat gatgaccctt atggagatct
     7621 tgtgtgggta aatacaaaag caagtagctg tagtgagatg attgcagaat ttgctttgat
     7681 gtttcctgaa tctttaaaga tgaacgttaa ggctgcacgg ttactttatg caggaatcgt
     7741 cggagataca gggcgctttt tgtatccctc cacgacgtcc cgtacattag agattgcttc
     7801 gatattgaga aattatccat ttgatgcaag tgctttgaac agagagattg aacaaatgcc
     7861 gatgaaagtc gctaaacttt ccggttatct ttatcaaaat atccaggtgg atgaaaatgg
     7921 tgcaggaaga ataatccttc cgcaagaaat tcttgataaa tatggaatcg atgattcaga
     7981 gacagcttcg atcgtttcac ttcctggagt gattgacgaa gtgctggctt gggggatttt
     8041 tgttcagcag ccttcagggt attaccgcgt acgtttacgt tctaaggggc cgatcatcaa
     8101 tgaattggct aaacgccatc atggaggagg ccatccatta gcaagtggtg cgaatgcaaa
     8161 agatttagca gaagtagagg aaatctataa agagatccaa gatatatgcc aatcgtataa
     8221 gagcagaaaa acagaatgat acttcgttcg aaaacgtgcc ttttgtagta aaaatcacaa
     8281 atgtatcttt tcaagatctt ctttttccgt tacaatagtg ctcactaaag attttaaagg
     8341 agaaagaaga tgacattacc aaattgccca gaatgtggct cagagtacac atatgaagat
     8401 cgaggcttgt tgatttgccc agaatgcggg aacgaatgga acaaagaaga acaagaaact
     8461 gtcatagaag acggattagt ggtattagat gcaaatggaa accgtctgtt agagggcgat
     8521 agcgtgacag tgattaaaga cttgaaagta aaaggtgcgt caggtgccat taaacaagga
     8581 acaaaagtca agaacatccg tttagtagaa ggcgatcata atatcgattg caaggtagaa
     8641 ggatttggac cgatgaaact gaaatcggaa tttgtgaaaa agaattaaag aatattcaag
     8701 gtcttttcac attcttttcc tactactaat gataaaataa agtaaacgaa aaggagtgaa
     8761 acgaaaatgt atggttatgg aatgtatggt tttttagatc cgacatttct tttggtcatt
     8821 cttggattag ttatttccgg aattgcgtca gcgtacgtca atagtacttt ccgaaaatac
     8881 gatcaagtga gaagcaaaaa caatgtgact ggtacacaag cagcacaata tatattacaa
     8941 agtcaacaga taaataatgt cggtgttcaa caaattgccg gagatctcac agataactac
     9001 aattcaggta ataagatgtt gagtttgtct caagcaactg cacagtctac ttcagttgct
     9061 gcgataggtg tagcagctca tgaatgcgga catgcggtac aagatgcttc gggttatgtc
     9121 ccattgaaga ttcgtgcagc acttgttcca gcagctaact ttggttcgat gatttcgttc
     9181 cccttgatta tggtaggtgt attgcttagc tggaactcta cgttaatcaa tatcgggatt
     9241 ttcgcttttt ctttagcatt acttttccaa ttggtgacat taccagtcga atttaacgcg
     9301 tctcggcgag caatccaaat tttgagtgaa ggcggactac tgacggaaga agaagtaccg
     9361 atggcaagac acgtattatt tgcagcagca ctgacttatg ttgcagcagc gctttcgaca
     9421 ttcctgcagc tgttgcgctt gatcttattg ttcggcggca accgaagaga ttaaaataaa
     9481 gaagttgtga aatcagcgct ttgatctgag ctaataccga gagttagctg attgaatacc
     9541 atttatttgg aataagggct ctttgtcaat aaggactgat gggttgtaca aaatcaaatc
     9601 tgggtcagaa tgaacctcat tctggctcag
//
LOCUS       DACDSY010000109         9399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDSY010000109 DACDSY010000000
VERSION     DACDSY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.110"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            99..293
                     /locus_tag="IWK44_002164"
     CDS             99..293
                     /locus_tag="IWK44_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550659.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="IWK44_002165"
     CDS             562..861
                     /locus_tag="IWK44_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550660.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="IWK44_002166"
     CDS             complement(958..1392)
                     /locus_tag="IWK44_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9550661.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="IWK44_002167"
     CDS             complement(1624..2334)
                     /locus_tag="IWK44_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9550662.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="IWK44_002168"
     CDS             complement(2324..3184)
                     /locus_tag="IWK44_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP9550663.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="IWK44_002169"
     CDS             complement(3189..3827)
                     /locus_tag="IWK44_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP9550664.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="IWK44_002170"
     CDS             complement(3842..4141)
                     /locus_tag="IWK44_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9550665.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="IWK44_002171"
     CDS             complement(4184..5668)
                     /locus_tag="IWK44_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9550666.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="IWK44_002172"
     CDS             complement(5688..6149)
                     /locus_tag="IWK44_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9550667.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="IWK44_002173"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="IWK44_002173"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP9550668.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="IWK44_002174"
     CDS             7452..8222
                     /locus_tag="IWK44_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9550669.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="IWK44_002175"
     CDS             complement(8495..9322)
                     /locus_tag="IWK44_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9550670.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2919 a         1930 c         1477 g         3073 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgt
//
LOCUS       DACDSY010000110         9388 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDSY010000110 DACDSY010000000
VERSION     DACDSY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.111"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            104..1939
                     /gene="tdc"
                     /locus_tag="IWK44_002176"
     CDS             104..1939
                     /gene="tdc"
                     /locus_tag="IWK44_002176"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9550671.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2244..3722
                     /locus_tag="IWK44_002177"
     CDS             2244..3722
                     /locus_tag="IWK44_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9550672.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3688..5019
                     /locus_tag="IWK44_002178"
     CDS             3688..5019
                     /locus_tag="IWK44_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9550673.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5133..7685
                     /locus_tag="IWK44_002179"
     CDS             5133..7685
                     /locus_tag="IWK44_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP9550674.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7692..9131)
                     /locus_tag="IWK44_002180"
     CDS             complement(7692..9131)
                     /locus_tag="IWK44_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9550675.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
BASE COUNT         2965 a         1569 c         2007 g         2847 t
ORIGIN      
        1 tataaaaaaa aatttctcat ttttggcgct atagtgaaga ggtgttacag ataaacacat
       61 gtatctgcaa gacattgacc aacaaaatat ggagggaaga aaaatgaaag atatggatat
      121 caaggccgtc tttataggcg acaaagctga aaatggacca gtttataaaa tgttgttgaa
      181 caagatggta gatgaacatc ttggatggag agaaaattat attccttcag atatgcctgc
      241 aattagcgaa ggagataagc tcactccaga ttatctagcc acccgcgacc atatgataga
      301 ggtcttggat gaagtcagtc agcgattacg cgcaggatcg atcccatggc attcagcagg
      361 ccgttactgg ggacaaatga atgctgaaac attgatgcct gctttattag cttacaacta
      421 tgcgatgctg tggaacccaa ataatgttgc attggaatct tctatggcaa cttcgcaaat
      481 ggaagcagaa gtaggacagg attttgcttc attattcaat atgacagacg gatggggaca
      541 cattgcagca gatggttcta ttgctaatct tgaaggatta tggtacgccc gttgcatcaa
      601 atcgattcct ttagctgtca aggaagtgct gcctgaaaaa gtgaaaaaaa tgtctgagtg
      661 ggaacttttg aatttatctg ttgaagaaat tttggaaatg accgaaagct ttacggacga
      721 agaaatggat gaggtcaaag cagcttcttc ccgtagcgga aaaaatatcc aaagattagg
      781 gaaatggctg gtccctcaaa caaaacacta ttcttggatg aaggcgttag atatttgcgg
      841 agtcggctta gatcagatgg ttgctatccc agttcaagag gattatcgaa tggatattaa
      901 tgctttggaa aaaacaattc gtgaattagc tggacaaaaa atcccaattc taggtgtggt
      961 tgctgtcgta ggaacgacag aagaaggtca agtagacagt gtagataaaa tcgtccaatt
     1021 aagagaaagg ttaaaagatg aagggatcta tttctattta catgttgatg ccgcatatgg
     1081 cggctatgcg cgttcactgt ttctaaacga agcaggagaa tttgtgccgt atgcttcctt
     1141 agctgaattc tttgaagaac atcatgtttt ccaccactgc gtaacgattg acaaagaggt
     1201 atatgaaggg ttcagagcta tttcagaagc ggattctgtc acgatagatc ctcataagat
     1261 gggctatgtc ccttatgctg ctggaggaat cgtaatcaaa cataaaaata tgagaaatat
     1321 catttcttat ttcgcgccat atgtgtttga aaaatcagta aaggctccag atatgttagg
     1381 agcttatatt ttggaaggat caaaagctgg agcaaccgcc gccgcagtct ggacagcaca
     1441 tcgtgtattg ccattgaatg tgaccggata tggtcaattg attggggctt caatcgaggc
     1501 agctcaaaga ttcagagaat ttctcgatca tctgactttt actgttaaag gaaaaacaat
     1561 cgaagtctat ccattgaatc atcctgattt taatatggtc aattgggtat tcaaagaaca
     1621 aggctgtaca gatttgaacg ctatcaatga attaaatgaa aaaatgttcg atcggtcttc
     1681 ttatatggat ggcgatgttt atggtgaacg gtttattact tctcatacta catttacaca
     1741 agaagattac ggtgattctc cgattcgttt tgttgaaaga atgggtctaa cgaaagaaga
     1801 atggaaaaaa gaacagaaaa tcactctgct acgtgcggct atcatgactc cttatttgaa
     1861 tgatgatcga attttcaatt tttatacaaa aaaaatcgcc aaagcaatgg aaaagaaact
     1921 aaacgaaatc atccaataga actgattttt gtgtgaaaga ataacaagag acatatggag
     1981 aacagacaat tatttgcttc atcaaaacaa ttgtatttcc ccctgttttt catatattcc
     2041 tcgccaagat attctttttt ataaaacgtt ctataagttg gatcgttcgt ttcatgtatc
     2101 aaacccagtt cactaagatc gttcaatgga tgtttcggtg atactgggtt ttgtactgaa
     2161 tcgatgaaac cttgtcattt ctgttcttga acaaaaataa aagaaaacaa aaacatatgt
     2221 cgctattgaa agaaggaata aagatgaaag atactggttc atcaaagcaa atcacgacca
     2281 tgacctttat cggaatgacc tgcgcccttg tagcaagtgt acgaaatatt ccagatgtag
     2341 cagcaacgag ctggacgatg attttttaca tgctggttgc agtattattg tacgcttttc
     2401 caatctcatt gatctctggt gaatttgctg gaatgtttcc ccaaaaaggt ggtccagaac
     2461 tttgggtatc gaatgctcta ggaaaaaaat gggggttcgt tgtctcatgg ctgttatggg
     2521 tccaaatgtt tcctgggatg gtgatggtcg cttcagcact cgctccgttg tttggtaata
     2581 ttatcgataa tgtgccgctt ggtttgaaca gcaaatttac attagttgtc attttagtcg
     2641 tttattggat catcactctt ttgaatttga aatttgatat ggcaaaaatc ggcggaaaag
     2701 tcggtgtttg gttaggattg tacgtgcctt tgacgatgat gctcttatta gggtttgctg
     2761 cgtggatcaa agttgggatc gtaccaaccg gaacattagg cagtttttca tgggacaaat
     2821 taataccaga tactacgact gcatcctcat ttgtttattt tgcgccgatc atgtttattt
     2881 ttactggtat cgaaatgtcc tctgtgtata tcactcgatt ggaaaatcca gttaaaacgt
     2941 atattcgcgg agtatttgcc gcgttaattt ttatattttt cgtcaatatt ttgaatgctt
     3001 tagttgtagc aaatgtcgta ccaaaaggac aaatggaatt gaataatatc gcgcaatcta
     3061 tttcgattta ttgccaaatt cttggtttgc cacatcttat tgtgaatctg tttagtttac
     3121 ttgtcttcat tggtgtagca gtccaattat ctgcttgggc ctcaggacca gcaaagacag
     3181 tgacagaaag tgctagaaaa ggtgcgtatc caccaaaatt caatttttgg aagacgaatc
     3241 aatttgacgt atccaaatcc gttattttga ctcagtctgt cattatctca atctttgcac
     3301 ttttttattt gctgattcct ggcgtcaatc aagcattttt gatgctcgta aattcaacta
     3361 ctgttatcta ctgtatcgtc tatgtcatca tggggatcgc agttttgaga ttgcgttata
     3421 cacatgcaaa gttgaatcga ccatttagaa ttggaaaaaa agagaaaaag agcaatcttg
     3481 gagtttggac tgtcgttatt gttttatttg cggcaatcgg attttctgtc ggactgacga
     3541 tgaaagctgg aacatggatc aatctaattg cagtcacagc catcagtgtc attttgtttg
     3601 tagtaccact ctgtattgaa aaaataaaga aaccttcttg ggaacaggaa gtcgccatgc
     3661 tgcttgaaaa agaaaaaggt gaacttgatg gaaaaaccgg caatgaaacg gactctaact
     3721 aatcgaaata tccagttaat cgcattaggt ggcgcaattg gaacaggatt atttttaggt
     3781 tcagcaaagg caattgcatc agcagggcca gctgttattc tcgtttattt gatatgcggt
     3841 ttggctattt atggtttgat gcgggccatg ggtgatttat ttttagcaaa tagtacattt
     3901 aactctatca gtgatttcgt tgactattat ttaggcaaaa agtgggcatt ttttgtcaat
     3961 tggacttatt ggctttgctg gcttggagct ggacttgcgg aattaacagc aataggattg
     4021 tatatccatt tctggtttgt acatgtacca gctttgataa gcggaggtag cgcactaatg
     4081 atcttaatgg cagtcaacct tgttgcagtc aagtggtttg gtgaattgga atcttttttc
     4141 tcaatcatca agatcttggg gattttcctt tttatcttat tgggtatagg attgagtgtc
     4201 tcagattttc atacccagtc tatcggaatc aaagaaaatt ttggacagat cgatgaatta
     4261 ctaccgtttg gctttgctgg aatggtagtc tcatttccta tggttttatt cagttttgct
     4321 ggaattgaaa tgatcggctt aaccgttggc gagacaagtg atccgcaaaa gaatttgaaa
     4381 aaagcaatca actgtctgcc ttggcgtatt ctctttttct atataggaac gattttgtcg
     4441 attttatttg ttgttccatg gcattgttta gatttaaaag aaagtccttt agtgactatg
     4501 ctgcaagtga tccattgcca aggcggagca gcaattgtca atctaatgat tttggttgct
     4561 tctttgtcct cagcaaatag tgcagtctac agtactagca ggattttata tcaaacagca
     4621 aaagaaaaaa gtttgcccca ttggttttgt gcgctttcta agaaaggcgt acctttacac
     4681 ggtattttaa ttacaggaat gttatttttt gccggtcttt gtctacattt ttttgtagct
     4741 gatagtcgat tgttttttca gcttttggca ggcatgacaa cgagttgttt tctatttgtg
     4801 tggtcttcga tcttgtgtgc acatgttcaa tttattaaag aaaagaaacg gaacactaca
     4861 caaagatata aagatcttgc tttgttggtg ttttttactt caattgcttt cagcttgtta
     4921 ttcgaaagca cgacacgctt tattcctttt ttattggtta tctggtttgt attcttaaat
     4981 tttgtatata aaagaataag caatagaaat atcagttagg cccagataca ttttgcaaaa
     5041 cgaattattt cagacccccc tgaaataatc gatacgcctg aaaaaacttc cctgcgtata
     5101 ataacagtat caattaaaca ggaggggata ggatgaactg gtacagattg acggtagaag
     5161 atgtattgaa aaactatcaa acatcaacag aaggcttgtc gcaagaagaa cgacagaaac
     5221 gcctgactga tcaaggatat aatgaaataa aagcaaggca gcagacaaaa cgatggaaaa
     5281 aaatagcaaa gcattttact gatctgctga tgatcgtgtt gatcattgcc tcttttttga
     5341 aatttgcttc tagtgaatat attgaaggaa gtattatttt attcgtagtg atcgtcaatg
     5401 gactcgttag ttactggcaa gaaagaaaag cggaagaatc attgaatggt ttgaagcagc
     5461 tgatgggaca agaagcgata gtactatcaa acgggatgca agaaaaaatc cctgcgaaag
     5521 aactggtaaa aggagatgtc attgtcttac gtgccggaga tgtcgttcca gcagatatcc
     5581 gattaatcga agcacatgat ttattagtgg aagaatcgat tttgacggga gaatcagaag
     5641 catctgaaaa aagtcatgcc gttttgacag aagaagaatc gatcggcgac cagaaaaata
     5701 tggctttttc aggaacgctt gtacaatctg gttctgcagt cggtgtcgtt gtagaaacag
     5761 gaaacacgac agaaatcggt aaaatcaatc aagcattgca atcggtcgaa caacaaacga
     5821 cgcctctgat taaaaaaatc aaccgattaa acaaacaaat ttttcaaggc attctttgtt
     5881 tgagcctatt cttagttatt tttacgacat ttcgatatgg tatggattgg catatccttc
     5941 tttcggctac gattgctttg gtggtgtcaa tggtacccga aggattgcca gctgttttga
     6001 ccatgattct atccgttgga gtacatgaaa tggcaaaaga aaaggcgatc atcaaaggac
     6061 ttccttcggt agaaacatta ggttctatga cagtgatctg ttcagataag acaggaacat
     6121 tgacaaaaaa tgaaatgaca gttgtggatg tagctgcaaa agaagaagcc tgcgtgttgt
     6181 ccattatgaa aaactgtcag gaattaaaaa caaaagaaga acagaaaaca gaaaatttaa
     6241 gtggcaatcc gacagaagtt gcgctgattc gacacacaga aacagccaat ctacctttga
     6301 aaaaaataga agcaaagatt ccgttcagtt ctgaatacaa atatatggca acgatgcatg
     6361 cggaggaaaa aggagcggtg atttacgtca aaggtgcacc agaagtatta tttgctaaat
     6421 caactctttc ttctgcagaa caagaagcat ggagtcagac agctgcagaa tttgcgagaa
     6481 aaggtcagcg tgttttagga tttgcttata aaaaagtaga ttcgaaacag gaactcacgc
     6541 atgagacttt aacccaatta acttttgcag ggatcgccgg attgatcgat ccgccaaaag
     6601 aaagtgcagt caaagctgta aaggaatgcc aacaagcagg gatctcagtg aaaatgatta
     6661 caggggatca caaagacaca gcaaaagcga ttgcggaaca aattggctta aaacatactg
     6721 ccaaagtttt agaagggatc gatttggatc ttatgtctga tgaagagttg attcagcaag
     6781 tgccaatagt cgatgtcttt gctcgaacga caccagaaca taaactgcga attgttaaag
     6841 ctttgcagaa aaatggagaa atcgtcggaa tgaccggaga cggcgtaaat gatgcacctg
     6901 ctttgaaaag atccgatgta ggaattgcta tgggaatcaa aggtagtgaa gtaagcaaac
     6961 aggcagctga tatggtttta ggagatgata atttccatac gattgcaaaa gccgtaaaag
     7021 agggccggcg gattttggat aatttgcaaa aaaccatcaa tttcttcttg ccaactgcat
     7081 tagctcaagg attgatcttg atttgggcac ttatgctgaa tcgcccgcta cctttgtccc
     7141 cagtacagat tttatgggtc aatatggtaa cgacaatcac tttatcttat gcactagggt
     7201 tcgagtcgcc agatccagaa atcatgaagc gcccaccaag agatccaaag caaggaatcc
     7261 tatctggtta tcatttgttt cggatcatct atgtctccct actgatcatg gttcctgcct
     7321 atttattggc tatgcagttt gaagggcaaa gcttacagca aaccattctt ctgcaaaata
     7381 tcgtcttagc tcaagctgtt tacatgatca attgcagaga gttgtccaag ccgtcattga
     7441 accaaggtct tttgcaaaac aaagcgcttt ttctttcatt aggtatcctt gctttattgc
     7501 aagggatggt tatttttcta ccagtcggcc agcagctgat cggtacagta agtttaacga
     7561 tgacacaaca gttattgatt ggagcaaatg tgattcttct attcttagtt gtagaaatag
     7621 aaaaagggat catgaacaaa ctgtttcgca aaaaagaaaa aacagctaga gaatacagtc
     7681 gctagccgtt actataaaaa agttttgaaa gtgagttcag tcaaacgatt gagctcattt
     7741 ttcgttggaa tagaagaaat gtatacaata gggatatcta gagaatctag agggaaattg
     7801 gaaatcaaga tatctgtttt ttcaaaagaa atagtggaaa gatcggttag ttcgataggt
     7861 aagaagcaga cacgttttcc taagtaatct tttaattcct gctgcaagaa cgccttccat
     7921 gaaggttctc cttggaaaat aaaatagaca ttgaacaaat tcggtttgat agaaaaaatc
     7981 gtttgttgga tcagtagaga caagagttgg agaaagtaat ttggttcatt gatttctatt
     8041 tcttttttta gatcgttcag taaattctct ttctgttgat acaagtgtgc atattgctga
     8101 tcatatgttt ctaaaaaacg atggtattga cggtccaatg cttcaatcaa tatcggcgat
     8161 tcatgatatt ttaatatgag gagagccaag ttatcagtca gagatgtttt ttttagttga
     8221 tcaagagaca gctctgaagc tagttcctct acaaacgttt cgaggacctc taaaaatgga
     8281 gaatgatgca ggcagcgagc ggtttcttgt ccatagttat tgttgtaatt ccaactttcc
     8341 agaaagcaga aagaagcaaa agaaagctca gtgtctttaa gataaatacc ttccgttaaa
     8401 tacaattttt tcaatgcaga gtcatacagg gaggagagtg tgctgttggt atggatagaa
     8461 gaatctaatc ggcaattcgc ttttatacgg atcgtattga taaagaacca acatgttccg
     8521 aaaatctctt ctgtttcaac cgctagttca gtttgactga ggtttcttaa aaattgaaaa
     8581 taatgggatt catggatcga ataatcctcg aaaaaatagt tgttatgagt gaaaggaatc
     8641 agcaatcgat gataaaaagt acgaatcgca cgctcttttc caagaataca agctgtatat
     8701 ttcgtcaaat cgatctgcaa gtcgtaactt gctaaacgct gattgatttt agtcacatgt
     8761 cttttcaatg tcccataaga aagcccatgg tcggctagaa aatcagggac agcaactttt
     8821 tttgtaaaaa gcagattttt cagcagttcg acactgactg attgtttgaa aaaaatctcc
     8881 caaaggtcat ttgttaataa attttgctgg ttttgcaaac tgattcctgc ttgatcgctg
     8941 atgatgtccc agccttcggg cagctgagag cgaatagatt gaaggtcaga aaaaatcgtt
     9001 cgttcggttg tccctatgat ttcagctaat tcatttacgg ttatctttgt atgattcagc
     9061 aattgttcta ctaaagaaac atgacgtcga atatcttttt cggtaatgat ttttttcatt
     9121 agtgaataca tcaatcagac tcccttctat tctaagaata cggagaaaat aaacggtggt
     9181 caagtttcaa ctgatttttc aatcattgtt tatttttctg gagcatttac tgatgataag
     9241 aatttcatca tgaacttgaa aaatgatacc actaaaatca aaaaataggt gggcaaacaa
     9301 taataaacat tttaatagta gatggtagca agaccaacac aggcggattg aacatttaag
     9361 agttgataaa aaagaaaaat atcgaagt
//
LOCUS       DACDSY010000111         9360 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDSY010000111 DACDSY010000000
VERSION     DACDSY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.113"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(93..761)
                     /locus_tag="IWK44_002181"
     CDS             complement(93..761)
                     /locus_tag="IWK44_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9550676.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            895..1182
                     /locus_tag="IWK44_002182"
     CDS             895..1182
                     /locus_tag="IWK44_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550677.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1598..3136
                     /locus_tag="IWK44_002183"
     CDS             1598..3136
                     /locus_tag="IWK44_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP9550678.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3133..4098
                     /locus_tag="IWK44_002184"
     CDS             3133..4098
                     /locus_tag="IWK44_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9550679.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4085..5407
                     /locus_tag="IWK44_002185"
     CDS             4085..5407
                     /locus_tag="IWK44_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9550680.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5470..5574
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5648..6391
                     /locus_tag="IWK44_002186"
     CDS             5648..6391
                     /locus_tag="IWK44_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9550681.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6406..7398
                     /locus_tag="IWK44_002187"
     CDS             6406..7398
                     /locus_tag="IWK44_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9550682.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7410..8147
                     /locus_tag="IWK44_002188"
     CDS             7410..8147
                     /locus_tag="IWK44_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9550683.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8137..8859
                     /locus_tag="IWK44_002189"
     CDS             8137..8859
                     /locus_tag="IWK44_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9550684.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8943..>9360)
                     /locus_tag="IWK44_002190"
     CDS             complement(8943..>9360)
                     /locus_tag="IWK44_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP9550685.1"
                     /translation="SGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEKQ
                     AAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYLN
                     GDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3068 a         1607 c         1972 g         2713 t
ORIGIN      
        1 atttttttat aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc
       61 tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca
      121 agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt
      181 tccagatact tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac
      241 agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag
      301 aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct
      361 gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata
      421 cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc
      481 cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt
      541 tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca
      601 gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct
      661 gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa
      721 atgattccgc acaattctgt aaataaattt aattttttca tttctagcct cttttctttt
      781 gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg
      841 aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga
      901 gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt
      961 tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat
     1021 caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt
     1081 tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct
     1141 ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa
     1201 gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac
     1261 agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta
     1321 ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc
     1381 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
     1441 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc
     1501 ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa
     1561 atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt
     1621 aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat
     1681 ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa
     1741 aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc
     1801 cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact
     1861 tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga
     1921 cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa
     1981 agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt
     2041 aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat
     2101 tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc
     2161 cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga aacctcgata
     2221 ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc
     2281 tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat
     2341 cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa
     2401 accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat
     2461 ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa
     2521 aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca
     2581 attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa
     2641 gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga
     2701 tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat
     2761 ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta
     2821 ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct
     2881 caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg
     2941 cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa
     3001 taaattaagc ggaaaaataa cggaggatca aatgattgat atgaattacc aagtcaatgt
     3061 cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa
     3121 ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga
     3181 aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt
     3241 ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca
     3301 tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg
     3361 cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag
     3421 caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt
     3481 gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct
     3541 agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc
     3601 ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca
     3661 ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg
     3721 cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa
     3781 attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg
     3841 catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat
     3901 ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct
     3961 atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa
     4021 agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa
     4081 ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc
     4141 ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg
     4201 gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg
     4261 taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga
     4321 agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca
     4381 attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc
     4441 tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag
     4501 acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc
     4561 attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct
     4621 agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc
     4681 attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg
     4741 cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg
     4801 tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca
     4861 gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat
     4921 tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt
     4981 ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa
     5041 gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta
     5101 cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca
     5161 agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc
     5221 taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga
     5281 gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa
     5341 agagttggat agctatatca tggggtatcc gacattggca agtgatctct cctatttatt
     5401 gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta
     5461 aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta
     5521 gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc
     5581 gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg
     5641 taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg
     5701 gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt
     5761 attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa
     5821 tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat
     5881 ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt
     5941 gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa
     6001 agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt
     6061 gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa
     6121 gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt
     6181 ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg
     6241 cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc
     6301 gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa
     6361 agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg
     6421 cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc
     6481 aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact
     6541 aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat
     6601 gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa
     6661 gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat
     6721 ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta
     6781 tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa
     6841 agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca
     6901 aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat
     6961 atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta
     7021 aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt
     7081 acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg
     7141 aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa
     7201 tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat
     7261 tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca
     7321 gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact
     7381 aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag
     7441 tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta
     7501 catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag
     7561 tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata
     7621 cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa
     7681 acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt
     7741 atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg
     7801 ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc
     7861 ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt
     7921 tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag
     7981 caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg
     8041 agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga
     8101 agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat
     8161 ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc
     8221 aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa
     8281 gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag
     8341 ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat
     8401 gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat
     8461 gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa
     8521 gcttacccta atcactattt gatccattgg tattcttctt tggactatca acaagactat
     8581 attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg
     8641 caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca
     8701 gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa
     8761 cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact
     8821 tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta
     8881 gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt aataaatgaa
     8941 tcttagtacc aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt
     9001 gaagtaacat attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag
     9061 taagaagaat ctaattggta acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg
     9121 cttgattctt tttgtgcgat ccattctttt gcagagcttg tgcttgctgg agtagcagca
     9181 gtttctgtta cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt
     9241 tgaaccgttt cttgtacaac aggttgttca gcttggactg gtgcttcttg aacggctgct
     9301 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
//
LOCUS       DACDSY010000112         9333 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDSY010000112 DACDSY010000000
VERSION     DACDSY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.114"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            31..1455
                     /locus_tag="IWK44_002191"
     CDS             31..1455
                     /locus_tag="IWK44_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9550686.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            1754..2461
                     /locus_tag="IWK44_002192"
     CDS             1754..2461
                     /locus_tag="IWK44_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP9550687.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            2448..2771
                     /locus_tag="IWK44_002193"
     CDS             2448..2771
                     /locus_tag="IWK44_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9550688.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(2860..3897)
                     /locus_tag="IWK44_002194"
     CDS             complement(2860..3897)
                     /locus_tag="IWK44_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9550689.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(4218..5201)
                     /locus_tag="IWK44_002195"
     CDS             complement(4218..5201)
                     /locus_tag="IWK44_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP9550690.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(5616..6386)
                     /locus_tag="IWK44_002196"
     CDS             complement(5616..6386)
                     /locus_tag="IWK44_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9550691.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6379..7314)
                     /locus_tag="IWK44_002197"
     CDS             complement(6379..7314)
                     /locus_tag="IWK44_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9550692.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7630..9279)
                     /locus_tag="IWK44_002198"
     CDS             complement(7630..9279)
                     /locus_tag="IWK44_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9550693.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
BASE COUNT         2731 a         1953 c         1708 g         2941 t
ORIGIN      
        1 cattaagtgg aaacaggagg taaaaaaatt atgacaacta tgaaaaagct aacttttcca
       61 gatgattttt ggtggggttc cgcttggtca gctgagcaag cagaaggaaa aggagaaaca
      121 ggaaaagcag agactatctg ggatcgctgg tttaaagaac aaccttaccg gttttatcaa
      181 cgaatcgatt cctctattac aaccgatcac atccatcaat ataaagaaga tatagaacta
      241 atgaaaaaaa ctgggcataa ctcctttcgc gtttctattt cttgggcacg aatgtttcca
      301 aatggcggta ccggaaaagt aaatcccaaa gccatttctt tctatcgaaa attattttcc
      361 gaaatgaata ataacggaat caaagtattt gccaacttat atcattttga catgcctgct
      421 actcttcaag ataaaggagg atgggaatca cgtgaagtag ttgatgctta tgttcattac
      481 gctgaaactt gttttaaaga atttggtgac ttagtttgtc attggtttac cttcaatgaa
      541 ccacttgggc ctattttagg tacttatctt gaagattttc attatccaaa cctcattgac
      601 tttaaacgcg gagcgcaagc agcattcttc actatacttg cacacgcaaa agcaatacaa
      661 gtatttaaag aactagctct tcctagtaaa ataggcgtta ttctaaattt gagtcctact
      721 tttcctcgta gtcaaaatcc tgctgatgtg aaagcagcag aaatcgctga tttattttat
      781 acgcggagct ttttagatcc tatggttaaa gggaattttt ctaaaaagct tatcctgtta
      841 ttacaagaat ataatcagct tcctgaagat tatacgaaag aagatcttgt atgtatctac
      901 gaaaataccg ctcaaattct tggactaaac tattacgagc cacgtcgcgt caaatctcgt
      961 tcttctatgg tggatacatc tagtccattt ttaccagagt ggtttttcga accacatatc
     1021 atgcctggac gacgaatgaa cgaataccgg ggatgggaaa tttacgaaaa aggaatttat
     1081 gatttgtgca tggatatcaa gaaccattat ggaaacatcg aagcatttat ctcggagaac
     1141 ggaatgggcg ttgcggatga agaacgtttc atggatgata cagggcaaat aattgatgat
     1201 tatcggattg agtatatcaa agatcatctt gcttacttat ggaaggcaat aaatgaagga
     1261 tgtaacatta aggggtatca tctttggaca tttattgatt gctggtcatg gatcaacgcc
     1321 tataaaaacc gatatgggct ggtttcactc gaccttacta ctcaaaaaag aacaattaaa
     1381 aaaagtggcg aattttacaa gcaattaagt gatacaaatg gatttagata cgatgtagac
     1441 ttgttagttg aataatattc taaaattcca cttgaagtga acacattaga aaacaagaat
     1501 aatgagaaat accaataaca aatacttttt cttcatactt catttatact tgtcagtata
     1561 agagactgtg acaaatgtag tgatcctcag aagttaggtc tggaaatcta acttgagaag
     1621 atttttttaa actacgtcca gtctttttta tttgtttaaa attgaagttg acaagtcttc
     1681 attaaaaatt tagaatattc acaagtttga cgacaattat gaaaatttat gtcgtgctaa
     1741 aggagttttg ttattggatg aaaagttaga tataaaaact gcgatcaaag acacgttacc
     1801 taccgttttt ggttatatcg gtattggact tgcatttggt atcgttggga aagctgccgg
     1861 gtttcatccg ctggtcgtca cgttgatgtc cctgctgatc tatgctggtt ctgcccaatt
     1921 tatcacggtc agcatgcttg ctagccatag tccactgctc tccatcgttc tctcgacctt
     1981 tctagtcaat tcccgaatga ttctgatgag tatgacgatt gctccttatt tcaagaaaaa
     2041 tcggctgctt caaaatctgc tgattggcac attgctgaca gatgaaagtt tcgcactggg
     2101 aatgaataaa ttgaactata ctggacaaaa gttaaacttt cgttggatga atacagctaa
     2161 ttggatttct tatttgacgt gggtgggttc ttctcttgtt ggcgcacttt tagggaattt
     2221 tattactgat ccgaaaaagt ttggtttgga tttcgccatc gtcgctatgt ttatcgggtt
     2281 actttatctt caagtcattt ctgatagaaa cacctctaaa cgattacagt tgatcctgat
     2341 tggtctcact ttgattctgg tatatgtcgg actgattttt atcccaagta acttagtgat
     2401 tttagttgta acactgattg ggtgcggatt gggggtgtgg atcaaacatg ccttcttttg
     2461 agtttacatt gctgactatt ttaggttgta ctttagcaac ttggctttct aggatcctac
     2521 catttgtctt actgaaaaaa ttcgatttgc cgcaaccgtt gcttgagtat ttaagttttg
     2581 tcccgatcgt cattatgtct gctttatggt tcagcagtct atttacccaa aatatcggac
     2641 atctgccgca gatcaatctc gaaaatgctt tggcttcagt tcctacactt ttagctgcta
     2701 ttctttcaaa aagcttattg gtgatcgtct tggtaggaat tctttccctt tcgttgattc
     2761 gcttattata aataaaaaaa caagagctta tggcaaatta ctgtcatgcg ctcttgtttt
     2821 tatcaaagtc aaataagaaa aaaatcggcg tattcttgct tatccgttga acggaccact
     2881 aacaacgact tttcctaata tatgtcctgt ttctacgtct ttggttgctt gtttcaagtt
     2941 cttcgcattc atcccatctg aatagacctt gctcaatgta ctgcggataa tcccttcatt
     3001 tgctaaagca gcaatgtgcg ctaaagcttc tccttgcgtt tcgatttcat agttatagtc
     3061 tgttttagcg aacatatact cccagtcgaa gctgatagat tggtttttcc aaacacctat
     3121 atctaacggc tctttgatcc ctacgatcgt tcccacatgt ccaaatggtc ggacaaggtg
     3181 tttgatttga tcaaagtagc gggtaatatc gaataataca gcgacatact ctacttgatc
     3241 gattcctaat tcacgaaaac ttttacttaa gtcgtgatga taatcgattg gatagtctac
     3301 acctgttttt ttcagccatt caaaattctt cggacttgca gtcgcgtaga cttctaggcc
     3361 agcccaatgc gctagttgat tcaaaatcga accgacgccc ccagctccgt tgatcactaa
     3421 gatttttttc ccgcggttgg cattttcttc tggagtcaga ccaaattttt caaataacaa
     3481 ttcataagcg gtcaatgagg tcaacggcaa ggctgcggct tcttcatctg aaagattttt
     3541 tggtgcaaga gcgacaatac gttcgtcaac cagttggtat tcttgattgc ttcctgctct
     3601 tgtcgttgta cctgcgtaaa aaacgcgatc gccgacagaa aatttttgca cttcatcacc
     3661 tacagcaaca actttcccga ctccatcaaa ccctaagata cgtaaagcat ttcttatccc
     3721 gttatcttgg cgaagtttcg tatcgacagg attaacagat actgcgttga cttttaccag
     3781 aagatcccgt gaactgggta caggtatctg ttctttttca tcaataaagc tttttggatc
     3841 atcgatcgac aacccttcat aaaaaccgac taccttcatt aattcctttt ctgacatacg
     3901 aattcctcct tagcttcatc aagttggagc atccatacag ttttcatttc aatatatata
     3961 gtatgctatt tgttccactt ttattcatca taattattct cttacttaaa gtaaactatt
     4021 tgcacttttt tggttaaaaa agaggttgcg acaagacttt tgtcacaacc tcttttaaac
     4081 ggtgttcaat caactgaccc tcggtattcg ctcaaaaaac gacaaaatat gaggagctat
     4141 ttttgtttgg ctgttcttat tacttgatgc aggacagctt tttcacgacc tctaattcac
     4201 ggtgttcaag caagaaatca ttgttcactg aaaagcgttg gatgttcttt ttcaaaggtg
     4261 tctaagatct ctcgtacttc atcagtagat ttcgtattca tcaactgaac tcgcaagtca
     4321 tttgctcctg gaaatccttt gatatagatt ttaaagaaac gatgtagtcc tgtgatggaa
     4381 cgcgggatga tttctgaata atgatcttgc agatctagtt gcagacgaag tagtccgatc
     4441 agttcttttt ctgtatgttc cctcggttct ttttcaaatg cataaggatt cttgaaaatc
     4501 ccccggccaa tcatgatgcc atctacgcca tatttttcgg ccaattcttc tcctgtctgg
     4561 cgatctggga tatctccgtt gatcgtgatc agtgtttgcg gagcgatttg gtcacgtaaa
     4621 gcaatgattt tagggatgac ttcccaatgt gcatctactt tactcatctc tttacgcgta
     4681 cgcaaatgga cagaaagatt ggctatatct tgatgaagta ggtgactgat ccattcttcc
     4741 atctcagaca tttccgtata gccgatacgc gtcttgacgc tgacaggcaa accgccagct
     4801 ttagccgctt caatcaattg tgccgctact tctgggcgaa ggatcaagcc actgcctttt
     4861 ccacgttctg ctacattagg gactgggcag cccatgttga tatcgattcc ttggaagccc
     4921 atttctgcca tagcaatgct catttctcta aaaaattctg gcttgtctcc ccaaatatgg
     4981 gcaaccatcg gctgttcatc ttctgtaaag gtcaatcttc ctcgtacact gtcttttcca
     5041 tctggatgac agaaactgtc tgagtttgta aactcagtaa agaagacatc cggtgctgct
     5101 gcatgcttta cgacatggcg aaatacaaca tctgttacat cttccattgg tgctaaaaca
     5161 aaaaaaggct ttggcagctc tttccaaaaa ttagtagtca tttatttgtt actctccatt
     5221 ctatcaagca gtgaagtcgc aatctttctc aaaaacattg tcattatact aggaaacaaa
     5281 aaaagaagca aagtgaacag cttaaaagat gtttctgatt gagcaaaaaa agactgtgac
     5341 aaaaatctta tcacaattcc ttttccggaa taaatggtgt tcaaaagctt gcatctgcgg
     5401 taataagtcc aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac
     5461 ttgatgcaga acagcttttt cacaacctct ccactaaacg gtgttcaatc agctgaccct
     5521 cggtattagc tcaacaaacg acaaaatatg aggaactatt ttcccgtttg tttctctaat
     5581 actcaggtca aaacgctgat ttcacaacct cttttctaac ttctcaatcc tcttccttta
     5641 ttgatcaaat accaagtaac ggcgtacaaa acaacgatga atagcacaag tacgatcatc
     5701 gatgttgcta ctggcacatc gattgttcca aggaatccgt aacggaaacc ggagatcata
     5761 taaacgatcg gattgacttt cgaaacagct tgccaaaatg gcggcaacat agagatggca
     5821 tagaaaacgc cacctagata agttaacggc tgcaagacaa atgtaggcac gatcgataca
     5881 tcgtcataag actgagcaaa aatcccgttg agcagtcctg ctaacgaaaa caagattgcc
     5941 gtcatcaata atgtaatgat gaccatgaac catgaataca catgaagtgg tacaaagaac
     6001 aaggaaatga tcgttacgag agagccgacg agaatactgc gacccactcc gccaatcaca
     6061 aagccccaaa tgatcacatg cgtaggaaca ggcgctacca agatttcttc gatgtttttc
     6121 tggaattttt gagagaagaa ggacgaagaa acgttggcat acgaactagt gatagcagac
     6181 atcataatca aaccaggcac gatgaattcc atatatgaaa aaccacccat gtcaccgatc
     6241 cgaccaccga tcatttttcc gaaaataaca aagtacaagg atgtcgtgat cacaggcgga
     6301 accagtgttt ggacccagat acgcaaatag cggttcgttt ccttgatagc taaacttttt
     6361 aatgcagtaa aataaagatt aaacattttt ctcctccgta tgattatttt cttctgtgat
     6421 tttcaaaaac agttcttcta aacgattcga cttattcctc atagaaagaa ctttgattcc
     6481 ttgtcttgtc agctgatcga agattccgtt caccccttga tttcgttcaa cttcgacagc
     6541 gattgtctgt tcatcttcaa atgtacaagc gtatccttca atcacaggtt tggtctcata
     6601 tggcgctaaa tcaaagataa atgtttcaaa ttgcagtttg gatagaagct ctttcatact
     6661 agtattctcg atcagttcac ctgattggat gatcccgatg ttgcgacaaa gcatttcagc
     6721 ttcttctaaa taatgcgtcg tcaaaatgat cgttgtacca ttttcgttca attctcggag
     6781 aaactcccac atctcgcgtc tcaattcaat atcgactcct gctgttggtt catccaagat
     6841 cagcaaacgt ggttcatgca tcaatgccct agcaatcatc agtcttcgct tcattccccc
     6901 ggaaagcata cgtgcacgga catttctctt ttcccaaaga ttggactgct tcaaatattt
     6961 ttcgctgcgt ttcatcgctt ctttgcgaga gacaccgtag tagcctgctt gattcacgac
     7021 gatttgctgg accgtttcaa agggattgaa attgaactct tgcgggacta atccaatctg
     7081 ttgctttgct cggactagat ccgtatccag atcataaccg aagacattca cttttccaga
     7141 tgttttattg actaaagaag taatgattcc gatcgttgta gacttcccag ctccatttgg
     7201 tcctaatagc gcatagaaat ctccttcttt cacggaaaga tcgattcctc gcaatgcttc
     7261 gaccccactt ccataaacct ttttcaaatt atttatttca agcgcatttg ccatttattt
     7321 cctatctcct tatagacatg attctttatt gtatcatgga atacagcaga tgaaaaaaca
     7381 acttggttcc agcgaaaaat caggttgctg ggctcaggaa aatctcctaa tgaaagacat
     7441 caatcggacg aagtcagaca accttttcag aaaaaacaaa tcgaagataa aggattacta
     7501 ttcatatgtg caacaaaagg agcgtgagac aaaagtaaag aacacttttg tctcacgctc
     7561 taaacacgta taaacggcgg aagcaaaagc caagattttt gatccaataa atactttcga
     7621 tcacttcgcc tatcccaata catgccactg ccgcttttca tcaaataacg cggcttccgt
     7681 ttttgcggtt tctactaaag tctctgggta atccaaaact ttcagcaagg caatcgcatt
     7741 tcgggtcctt gaaggtcctt cttttaattt gaaatcaaaa ctgattcctt ttccttctgt
     7801 tacttgttct tcaaagtgga cattctcaca ataattcttt aaaatctctg tcaattcgat
     7861 atcatgtgtg gcgacaaatg ctaaagatgg atactccgca agccaccgaa cgactgaagc
     7921 ggaagaagca atccgttcga tcgtatttgt cccttttaaa atctcatcta cgaagcagta
     7981 gcagcgttct ttggaagcca cttggtccaa caaacgtttg atcgatttga tttctgaaac
     8041 aaaataactg tcgccttcaa ataaatcatc ttcgattgcc atggaagtca acacatgtcc
     8101 aggttgcagg gtaaactgtt ctgcaatcgc tgtttggatc gtctgtgcta gaatacagct
     8161 gatggctata cttttgacat acgtagattt tcctgatgca ttggaacccg tgactaatgt
     8221 attcttctgc cagttgaccg gattcactac cgcttctttt aacaatggat gatagatatc
     8281 tttcgctgtc acaccgccct cagtaaactc tggtcggcaa gtgatgggca tataagtacg
     8341 gtaattcaat attgcagcgg ctacttctaa ttctcccagc aattcccaca gcttgatggc
     8401 ttcagttgaa tgcttttcta atttagtgag aacgaaatga taggagataa atggcagcat
     8461 gaacatgatg ttgacatatt caaaaatcaa ctctgcttca ctcccactct tcatacgaaa
     8521 tgaaaaacct aatttaggga tgtctcttaa tggtttagtt tgctgtttca gctcttcttg
     8581 aagtggtgtc ggcagttttg ctagctgatt ggcggctgaa atcgttgaaa ccaaataact
     8641 catgctattc agctctgttt ctagtgtttg tttcttcact agataataga ccgtattgaa
     8701 tactaaccca ccgattgcca aatagattcc tagtacttgt cctaaaagaa gcaaacccaa
     8761 acccaccagc ggaagcaagc caagaaaaat aaataaacct acgcgtccaa tctttttact
     8821 ttttccatcc gctagatatt gttttgcaaa attatgatct ttttttccta aacgagcgaa
     8881 ttgatattgg attttttctc tcagttgagg attttcctga taaaaagcaa tcagtttttc
     8941 taactgctga tcttcaccaa aatcaaagct gcgtaaacgt tgatacaacg cttctgaacc
     9001 tacactagaa taggtactgt tgattccttc gaatacttcg aacatatcca ggtcatacca
     9061 agtaagatca tcgatttcgc tatcccatga acggaacttc ttctccgtga gccatgcttc
     9121 cttcaagctt tcttctttat caaatctcgg ttgataagga attttttccc atttgctacg
     9181 aactgtttcc tttaatttca aacgtgaaaa aatgtctaaa gcaactaata aaataacagc
     9241 tgcaataata attaaaacga taaattctct ttgacccata tccctctccg ttcctttatt
     9301 tgttagcttt agtatagcac tgttgcgcta ttt
//
LOCUS       DACDSY010000113         9317 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDSY010000113 DACDSY010000000
VERSION     DACDSY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.115"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..626)
                     /locus_tag="IWK44_002199"
     CDS             complement(<1..626)
                     /locus_tag="IWK44_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9550694.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            899..3370
                     /locus_tag="IWK44_002200"
     CDS             899..3370
                     /locus_tag="IWK44_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9550695.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3473..5209)
                     /locus_tag="IWK44_002201"
     CDS             complement(3473..5209)
                     /locus_tag="IWK44_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP9550696.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5206..5910)
                     /locus_tag="IWK44_002202"
     CDS             complement(5206..5910)
                     /locus_tag="IWK44_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9550697.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6041..6544
                     /locus_tag="IWK44_002203"
     CDS             6041..6544
                     /locus_tag="IWK44_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP9550698.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6504..6842
                     /locus_tag="IWK44_002204"
     CDS             6504..6842
                     /locus_tag="IWK44_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP9550699.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6863..8281
                     /gene="ffh"
                     /locus_tag="IWK44_002205"
     CDS             6863..8281
                     /gene="ffh"
                     /locus_tag="IWK44_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP9550700.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8393..8971
                     /locus_tag="IWK44_002206"
     CDS             8393..8971
                     /locus_tag="IWK44_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP9550701.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8975..>9317
                     /locus_tag="IWK44_002207"
     CDS             8975..>9317
                     /locus_tag="IWK44_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9550702.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGG"
BASE COUNT         3009 a         1715 c         1952 g         2641 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagaa
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      481 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      781 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      841 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      901 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      961 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
     1021 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
     1081 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1141 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1201 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1261 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1321 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1381 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1441 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1501 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1561 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1621 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1681 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1741 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1801 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1861 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1921 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1981 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     2041 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     2101 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2161 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2221 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2281 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2341 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2401 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2461 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2521 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2581 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2641 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2701 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2761 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2821 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2881 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2941 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     3001 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     3061 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3121 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3181 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3241 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3301 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3361 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3421 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3481 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3541 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3601 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3661 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3721 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3781 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3841 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3901 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3961 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     4021 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     4081 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4141 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4201 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4261 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4321 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4381 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4441 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4501 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4561 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4621 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4681 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4741 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4801 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4861 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4921 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4981 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     5041 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     5101 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5161 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5221 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5281 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5341 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5401 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5461 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5521 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5581 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5641 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5701 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5761 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5821 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5881 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5941 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     6001 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     6061 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6121 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6181 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6241 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6301 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6361 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6421 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6481 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6541 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6601 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6661 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6721 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6781 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6841 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6901 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6961 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     7021 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     7081 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7141 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7201 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7261 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7321 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7381 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7441 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7501 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7561 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7621 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7681 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7741 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7801 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7861 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7921 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7981 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     8041 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     8101 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8161 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8221 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8281 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8341 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8401 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8461 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8521 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8581 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8641 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8701 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8761 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8821 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8881 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8941 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     9001 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     9061 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9121 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9181 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9241 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9301 ggttgtgggt ggcggtc
//
LOCUS       DACDSY010000114         9214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDSY010000114 DACDSY010000000
VERSION     DACDSY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.116"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..179)
                     /locus_tag="IWK44_002208"
     CDS             complement(<1..179)
                     /locus_tag="IWK44_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9550703.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(192..1295)
                     /locus_tag="IWK44_002209"
     CDS             complement(192..1295)
                     /locus_tag="IWK44_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9550704.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1385..2320)
                     /locus_tag="IWK44_002210"
     CDS             complement(1385..2320)
                     /locus_tag="IWK44_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP9550705.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2622..4556
                     /locus_tag="IWK44_002211"
     CDS             2622..4556
                     /locus_tag="IWK44_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9550706.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5013..5429
                     /locus_tag="IWK44_002212"
     CDS             5013..5429
                     /locus_tag="IWK44_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9550707.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5443..6009
                     /locus_tag="IWK44_002213"
     CDS             5443..6009
                     /locus_tag="IWK44_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP9550708.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6032..7018
                     /locus_tag="IWK44_002214"
     CDS             6032..7018
                     /locus_tag="IWK44_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9550709.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7173..8984)
                     /gene="scm"
                     /locus_tag="IWK44_002215"
     CDS             complement(7173..8984)
                     /gene="scm"
                     /locus_tag="IWK44_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9550710.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2617 a         1748 c         1879 g         2970 t
ORIGIN      
        1 ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa taaaaaagtg
       61 tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac cgcaaaagga
      121 aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt tagtgtcatg
      181 gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga gtcctccgat
      241 aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt tgtcacgaag
      301 atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt tttcacaccc
      361 attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt tatgatcgtg
      421 acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa atgatggtat
      481 tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa tgttttgttc
      541 cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg gatgccgact
      601 gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt accgcccaag
      661 tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc gtccgtaatg
      721 taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag ataaacagtt
      781 ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc ctgatcgatt
      841 tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg tgtgattgca
      901 ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc ccgcttgact
      961 aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc attcacttca
     1021 tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt tactcgatcg
     1081 aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg aacgtaagtc
     1141 cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc tgctgaactt
     1201 tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa agagatgccc
     1261 agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc attttgaaag
     1321 gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta gtatgattat
     1381 gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg cctttttcag
     1441 ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt gtagcacctg
     1501 taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg acgaattcgt
     1561 cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc atattttcat
     1621 tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca aagtttgatt
     1681 cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg ttagagtagt
     1741 tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg atttcgattg
     1801 tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt tggaagcttt
     1861 cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt gcttttaaga
     1921 attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt gcttccatgt
     1981 ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg ttatcatatt
     2041 ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg taaccatttg
     2101 aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca gctacaactt
     2161 ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat gattcagaag
     2221 aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga gctgaaaatg
     2281 ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc caccattcca
     2341 tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt tagcctataa
     2401 gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt cagcaaattt
     2461 cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta gacaaaagaa
     2521 cagaacatct atataatata tggagattgt gtaataagta tcaaactaaa agaattatca
     2581 tatattcatt atatagatag tctataagga gcggatgatc aatggcaaag caaaatattg
     2641 tcgttgtagg agcaggatat gccggtgttt cagctacaaa atacttggca aagaaactga
     2701 aaaaagaaga tgtagcaatt actctgatcg atcgtcattc gtaccatact atgatgaccg
     2761 agttgcatga agtagctggc ggacgtgtag aaccagaagc aatccaatac gatctgcagc
     2821 atttgtttgc tcgtcaaaag aatgtacaac ttgtcacaga cactgtaatc ggtatcgata
     2881 aagaacgaaa aatcgtcaag accaaattag gttcctatcc ttttgatcag ctgatccttg
     2941 gaatgggcgg agagccgaat gactttggta cacctggtgt caaagcacac ggattcaccc
     3001 tttggtcatt tgaagattct gtaaggattc gggaacatat tgagaagaca gtagcaaaag
     3061 ctgcattgga accagatgat gaactgcgta aagccatgct gactttcgtt gtctgtggtt
     3121 ctggatttac cggtattgaa atgattgggg aactgatcga ctggaaaaat cgcctagcca
     3181 aagattataa attagatcca aacgaaatca cactgatggt cgtagaggca atgccgacga
     3241 tcttgaatat gttggatcga aatgatgctg caaaagctga acgctatttg cgtaagcaca
     3301 acgtggaatt attattgaat gcaccaatcg tagaagtggc tgctgatcat atcaaattga
     3361 aagacggacg caacattccg actcacacac tgatttggac agctggtgtc aaaggaactt
     3421 ctgacgctgc tgactttgga ttagaatcag cacgcggaca gcggttgatc gccaatgaat
     3481 atatgcaggc aaaaggctac gaagataaaa acatctatat cattggtgat ttagtctttt
     3541 atgaagaatt cccaggaaca ccgacaccgc agatcgttca agctgctgaa caaacaggac
     3601 acacagccgc agcgaatatc gtggcttcga tcaaaggcgg agaaaagcat aaatttaaag
     3661 gaaactatca aggatttatg gtttctattg gtgccaaatg gggggtagct aacctatttg
     3721 ataagatcca tttaagcgga tttttagcta tcgtaatgaa acatatcgtg aatttgaaat
     3781 acttctttga tattcgttct ggttattata tgttccaata tttaatgcat gagtttttcc
     3841 atatcaaaga tgatcgcaac gtgactcgag gacattcttc tcgttacggc aatgtcttat
     3901 ggagtgtgcc acttcgtatt ttctatggtt tagtttggtt cgttgaagct tccaaaaaaa
     3961 tcattgggaa tggtgaagtg ttcaaaccaa gtacttggtt tggtgaagga tcatggttca
     4021 ctggaaacag tgtgttccct tggacatggg aatacaatgt aacagatgtt gccagtggtg
     4081 cttctgctgc tggtgatgca accacagctg ccagcggagc aggggcaagt gccggagaag
     4141 cagcgactca agctgcacat tttggtttga gttatgcata cggtgaagaa ccaatggctg
     4201 tgattgagaa gtcaccagac tggttcacaa agatcatgca agtgatcatg ccaaacaatg
     4261 atgtagcaat gttcttccaa aaattcatgg tattctttga aattgccttg gcattagcat
     4321 tgattgctgg attattcaca tggctatgca gtgcgacaac gattgtattg gtgatcacgt
     4381 tctgcttatc aggcatgttt tactgggtaa acatttggtt catttttgtt gctttcgcat
     4441 tgatgaacgg ttccggacgt gcgattgggt tggatcgctg ggtaattcca tggctacaac
     4501 gtacactagg taattggtgg tacggtagac caagttccca gtacggcaag aaataaatag
     4561 ataaagaacg ttatttgaat gcaagatttc aatagaaagg ggctgagaca aaccgtcaca
     4621 gcctcctttt tatgtaaaat taaaggaata agatgcccga gataagcctc catgagcaag
     4681 acacaggtgt cacaacctct gttacaacct cttgttataa acggtgttcc agaagtagct
     4741 tcttcgaaaa ataagccgaa atttcacaaa aacttgaaaa gtaattttcg taaattcctt
     4801 cttatttctt gaagttgccc acttctgtca caacctcttg ttataaacgg tgttccacct
     4861 gctgcttctc ggtaataagc ccaaagaatt agaaaacatg gaaagctgtt ttctattctt
     4921 cgttcttatt actcaaagca agacacaggt gtcacaacct cttgttttta tgctaaaatt
     4981 gggtgagtta atctggaaaa aggagaacaa aaatgagcct taaagaattt gctgaaaaaa
     5041 gtctgctcaa accttgggat gtttttatta ttttgctttt gactgttctt tcttttttac
     5101 cagtcatgat ttttggtctt cagcaaaaca ccgcaacagc agaaaaagaa gcggtactgc
     5161 gtgtggatgg cacagaaatc aaaacattcc ctttgatggc tggagaagag agcaacacat
     5221 atacttacaa agatgaacac ggagattata acttgatcga gatagatggc gatcgagtac
     5281 ggataaaaga agcagattgc gatgaccaga tctgcgtacg tcgaggctgg gcaacgaaaa
     5341 atggcgaaac catcgtctgt ttgccacata aattagtgat cgaagtccgt tcaacagatg
     5401 gaggtgacga agacagtctg atttattaga ccttcacaca ccatgagtaa attacaaaaa
     5461 atgatttata tttcgctact tgttgcacaa ggagtgatca ttggtttatt ggaaaatatg
     5521 atcccttatc ctttttcttt tgcgcctggt gcaaaactag gcttagccaa tctaatcacg
     5581 atcgttgcca tttttaccat gaaaaaacaa gacagcttct tactactttg gctacgacta
     5641 ttcctgacta ctttgctagg cggaacgatt tccacattcc tttatagtat gagtggtgct
     5701 ttgcttagtt attttggaat gttattggtc aagcaattag gaccaaaacg tgtcagtatc
     5761 atcggaatta gtgcggccgg cggtttcatg cataatgtcg gacagctttt gatggcttct
     5821 ttcattgcac aatcatggac agtcatgttg tacttgccag tcctttcagt tttagggatt
     5881 ttatcaggga ttgcaatcgg gatcgcagcg aattatcttt tacgacatgt gcagacactt
     5941 caacgattcc aaaaagatta tgagcaggga accaaacaaa aatggagccc aatgtttacc
     6001 aaaaaataat cgtcacacaa ggagtttttt tatgaagcta catccaatgt gggaaaacta
     6061 tcctgcttta agtaaagaat tgacagaaac actaaaacgg atggaagaaa ctgtccgcct
     6121 gaaaaacaaa cctgtcgaac aagccatcaa agaaacgatc catgccggcg gaaagctgtt
     6181 gcgtcctgcc tatcagctat tgttctcaca atttggacca gagcaagatc gagacaaagc
     6241 catcgctttg gcagcttcta tcgaaatgct ccatacggcc acactgatcc atgatgatat
     6301 cgttgatgaa gcggatctgc gacgtggtca gcctaatatc cgtagtcagt ttggtaatgt
     6361 cgtttctgtc tatgcaggag actatttatt tgtctgctgt tttaaactat tgtctgatta
     6421 ctccacttcc ttgaaaagtc tccaattgaa ttctcgcagt atggaaaaag tattgaatgg
     6481 cgaattaggg cagatggatg atcgctataa ttatgaactt tctgtaaaag aatatttgga
     6541 aaatatttca ggtaagacag ctgaactgtt ccaattaagt tgttctgttg gcgcttttga
     6601 aagtggaaca agcgagcgct tcgcaaaaaa agcaggagat atcggacttt ctatcgggat
     6661 ggcctttcag attatcgatg atattttaga ttatacaaaa gagagccagg aaatcggcaa
     6721 acctgtacta gaagacatgc gtcaaggggt ctattcttta ccgttgatct actctttgca
     6781 aaaaaacaaa ccaaagctac taccttattt agagaagaaa gcggcaatga cagaagaaga
     6841 tgtcgacgcc gtacgcaaaa tcgttgaaca tacaggaggc gtggaagaag ctaggaagtt
     6901 agcggcctct tacacagaaa aggctttgaa ggaaataaag aaattacctg ccacctctct
     6961 tcgaacaaaa gagaatctct tttcccttac ccaactgatt ttggatcgca aagactgaat
     7021 agggaaaggt tcgatacgta aataaatttc tcagaaggaa atctacgtgt tttttgacaa
     7081 attgaattgt agttcttgta tttatgataa aaagcaaaaa taaagagcaa cgtccgttag
     7141 cgacgttgct cttttttatc ttgaatattt ttttaccttt tcgatttctt ataactcata
     7201 aaagtgattc ttaaaccgat tacaagtaat ataacgcctg cgagtgttat ccaattattc
     7261 gattgacttc cagttttagg aagtgctctt tttgatttgt ttccattgtc attgcttgtt
     7321 ttattagtta agttaaagtt cgtcctgtta tccgaacttg tttttgagaa ggagacactt
     7381 gcatgacgtg tatcgcttgt ggtagacgac gtgtctgtac tatcgcttga agttgatgaa
     7441 tctgagctat cacttgtggt agaggatgta tctgtactgt cacttgaagc tgacgtgtcc
     7501 gtgctatcac ttgtagtggt agatgaatct gagctatcac ttgtggtaga ggtagtatct
     7561 gtactgtcac ttgaagctga cgtgtccgtg ctatcacttg ttgtggtaga tgtatccgta
     7621 ctgtcgcttg aagctgacgt gtccgtgcta tcacttgtgg tagaggtagt atctgtactg
     7681 tcacttgaag ctgatgtgtc cgtgctatca cttgtggtag aggtagtatc tgtactgtcg
     7741 cttgaagctg acgtgtccgt gctgtcactt gtggtagagg tagtatctgt actgtcactt
     7801 gaagctgatg tgtccgtgct atcacttgtg gtagaggtag tatctgtact gtcgcttgaa
     7861 gctgatgtat ccgtgctatc acttgtggta gaggtagtat ctgtactgtc gcttgaagtt
     7921 gatgaatctg agctatcact tgtggtagat gtatctgtac tgtcgcttgt gtctgtcgta
     7981 tctgtaccat tgtcgctgat aaatatttcg ttaggatccc aacgattggt agctgcatct
     8041 aaaatcacat ttgttcctat aatcgttccg tcttggtttt gagtaatgcg gatagtagca
     8101 ttaggcgcaa ggattgttcc ttgaaaagga gcactgattg tcaaatcagt catctcattt
     8161 ccaaaattcc aagagatatt cgcatccgaa aaatcttctg tttcatgatt tgaacgatca
     8221 ttgtatttga ttggtgactg tacgttcaat gcagaagaac tgttgatgac attaaataca
     8281 actacttgat catttggatt aatgatttga agtggcgtat tcatcgttaa cacttctgca
     8341 tcgatattta ccaataaaaa cccggtatct gataaatcag ttaaatcgat tgtccgattg
     8401 ttcatgtctg gaaaatctgc agcagtatat gttttagctg gagtgatcgt tgcaagatca
     8461 tttgctgcta cattcaattt agcaaattcc tgctcgaaat caatatattc atgcccagca
     8521 cgatctttat aaaaatcatc tggagttaag tgatcaagcc tggatccatt gactgtagga
     8581 tgaccatctt gaagtgctac atccacgtta ctgccaacga caaatttatt cattcttgtg
     8641 ctacctgtag gtatgcctga agaattagcg aaagaatcag ttttttgtat ataatttatt
     8701 tcttggttca ataaaccttc atgaagctct gtaccaaaat tattttttgc atcaagttca
     8761 tgtgttgcta tatttccgtc tgtatgtgca ttcacagttg tcttattttt agcaaatacg
     8821 tggaattgac tagcaattcc taacaagcca tagttaccag ttagatcatt ataaatattc
     8881 ccagggtctt taggaatgta tgtttccgct gaaacggcca caccatacga aaagatattg
     8941 aacaacaaag caactaggac tagtaaactt gccctttttt tcatttttcc ccttcctttc
     9001 ctttcaaaca ttttcctatt atactctttt tctcctagtc ttaaactgtt cgtttgaaaa
     9061 atcattgttt atttaaagaa tattaatgaa acgttcatat tgttgcttct tgttcaattg
     9121 atagttcgac actatctttt tctttcaatc tccaaactat tgatgatggt gactgttaaa
     9181 gtgtaaaaaa taagggtgtg tagtaacata taag
//
LOCUS       DACDSY010000115         9180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDSY010000115 DACDSY010000000
VERSION     DACDSY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.117"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            70..2838
                     /locus_tag="IWK44_002216"
     CDS             70..2838
                     /locus_tag="IWK44_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9550711.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            2919..3338
                     /locus_tag="IWK44_002217"
     CDS             2919..3338
                     /locus_tag="IWK44_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP9550712.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            3350..3820
                     /locus_tag="IWK44_002218"
     CDS             3350..3820
                     /locus_tag="IWK44_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9550713.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            3837..4616
                     /locus_tag="IWK44_002219"
     CDS             3837..4616
                     /locus_tag="IWK44_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9550714.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            4603..5424
                     /locus_tag="IWK44_002220"
     CDS             4603..5424
                     /locus_tag="IWK44_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9550715.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            5441..6490
                     /locus_tag="IWK44_002221"
     CDS             5441..6490
                     /locus_tag="IWK44_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9550716.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            6503..7507
                     /locus_tag="IWK44_002222"
     CDS             6503..7507
                     /locus_tag="IWK44_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9550717.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8140..9159
                     /locus_tag="IWK44_002223"
     CDS             8140..9159
                     /locus_tag="IWK44_002223"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9550718.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3058 a         1538 c         1941 g         2643 t
ORIGIN      
        1 tcaattcttt attaaagatt ttattgacaa aatgaatccg ttttctatga tggaggaggg
       61 gtgaataagt tgttgaagaa ggaaatttta tgttacttag agaaccagac agcctttttc
      121 gatttagaac atatgaacga ggtctttaca gcacaaaggt tagctgatca ttttggggta
      181 aaaagaaaca cagtcagtca ttatttgaac cagttaaatg atgagcaact actagtcaaa
      241 atcaattctc gaccagtgat ttatttccac aaagaagctt ttgaaaaaca attttttaag
      301 ttacgcacga atttttattt ttctatcgat gatctcaaag aggaacagcc ttttttcgcg
      361 caaccaaaag acttattctc ttttatgatc ggatacgacg ccagtttgaa agaaagtatc
      421 gaacaaatca aaacagctct ttactatcca gatggcggat taccactttt gattacaggc
      481 gaaagtggca caggtaaaag ttatttagtt aatttggtct atcagtattg tctgcttcat
      541 gatctgctag aagattctgc accattcatc acttttaatt gtgcacaata tgcagataat
      601 cctgaacttt tgactagcaa tttgtttgga catgttaagg gagcgtacac tggtgcagaa
      661 gaaaataaaa aaggggcttt tgaagcagca gatggtggga tcttgttttt agacgaggta
      721 catcgcttaa caccagaagg tcaggaaaaa ctgttcacct atcttgatca aggtgtgatt
      781 tatcgtatgg gagaacctaa tttgtcacgc cggatcaaaa cacgtctctt ttttgctacg
      841 actgaagaga ttactagtca tttcttaaca actttcattc gaagaatacc tatccgaatc
      901 gaactacctg cacttaatca gcgtagtcgt aatgaacggc tagaactgat ttactctttt
      961 tttctcgaag aacaaagaaa aatggggagc cctttgtcag tcagtggacc agctttgtca
     1021 cttttaacaa gtcagaaatt atccggaaac ataggagagc tgaaaaatat cgtcaaagtt
     1081 tcagcagcaa aagcatatgc tgaacagcgt gaacagcctg aaatccatgt cacgatccat
     1141 catttgcaaa aagaactact tgcgcagcct attactaaaa atacagcaca gcaaggaatc
     1201 tatattacgc aagaccagac cttagagcaa ttaatggaga agcaacagcc cgaacagcag
     1261 cgaatcgtta aaagttttga gcaaatccta attgatttca agaaaaataa ctgtctgcta
     1321 tattcttgcg aaggaaaatt aaagcaagaa gtgattcaat tatttgattt tttgctgttt
     1381 gagaccagta gacaagaaaa acacgagctg ctggtttatt ggacacaatc tatccgagag
     1441 acctttcgac aaatggaaac tgcctatcaa atcaaattcg atggaaacag tgtctatgcc
     1501 ttgagttact atctctatca gcgaagcact atcaaatggt taccggaaga acatgatatt
     1561 atccaactaa tcaaagaatt agaacaacaa gtttctcagt cttatccatc tagttatcac
     1621 tatgtccaac gaattctgga attaagtaag atcaaattag atattgaaga cacaagtatg
     1681 gatcggattt tactgacgat ctatctaaaa aaagcgaaat ggtcaaaaga aaaacgactg
     1741 ccaaaagcga tgattgctgc tcacggttat gcaactgcta gcagtatggc aaatgtagta
     1801 aatcgtttat tacaagagga tctatttgaa tcatttgata tgccactaga tgtcacacca
     1861 cagcagattg cttcagaaat ccttgattat tgcgaaaaca gcgatgtttc aaatggattg
     1921 atcatattag tagacatggg atctctgaaa gaaatccatc agttttttaa aaaacagtta
     1981 tccgtaccgc ttttgatttt gaataatgtg acgacacctt tagcaatcac agtaggggaa
     2041 tgcctgcaaa aaaatgtatc attagaagag atagcagaag agactgttcg acagatccag
     2101 ccagaatggc gactgctcta tccagaggaa aacaaaccca aagcactcat tactacttgc
     2161 ttcactggaa tcggtacggc ggtccactta agtgaactgc tagaaaaaag cttacctacg
     2221 gcttgtcagt tgaaaatcat cccatatgaa tatcagcaat taaaagataa gaaaaacagt
     2281 gacccattat tttccatata cgaagtcgta ggaatgattg gcaccacaga tcctgatatc
     2341 accaatatcg actatctttc attagaagac ctcatctcgg gggaaaagat gtctgtacta
     2401 gctgaatggt tggacactac tatgaatgct tcagagaagg aaatattcaa tcagcgcatc
     2461 attcgtaatt tttcattgga aaaagtactg gattccgtga ctattctaga tacagagaag
     2521 gtgatgggag aaatcgatca ttttatgaga gaattagagg tgcagttgaa tcgaagcatc
     2581 agcaatccca aaaaattagc gttgtatgtc catgtgagct gtctcattga acgattgatc
     2641 cgacaaatgc caatcgaaac ctatcaagga ttagagaaat ggaagcagtg tcaaaaagaa
     2701 gggttatctg ctattaaatc tgcgtttagt gtcattgaag agcattatag tgtcatgatc
     2761 cccaattctg agattgccta tatctatgat attttgagtg aagatacaga gtttctctcg
     2821 atagaagaag aattttgaac ataagcaagt tgttgccact aattttggca tgcaacttgc
     2881 ttttttagta tgtgtaagtg aaaagaggag atgaaaaagt ggatagagaa attatcttag
     2941 cttcccatgg aaaatttgct tcagggatat tagattcatt agagctgatc tatgggaaaa
     3001 atcattctat tactgtattc gattgctata ctgatgagaa ttttgattta gcggaaacag
     3061 taaggcagct ttttatgagg tacaaagata aagaaatcat tgttctgacg gatctttttg
     3121 gcggaagtgt caacaacgaa ttcttacaat atatcaatcg ggatcatgtc tatctcgttg
     3181 ccggattgaa tttgccacta ttgattgagc tagtatctcg gttggacgct gaagtagaga
     3241 caactgtttt gatccagcag gtgctaactg attcaaaaaa aatgatccag ttttgtaacg
     3301 agcggctcaa tcagaaaata gaagaagacg aattttagga ggaagaaaaa tgattgtatt
     3361 gacaagagtc gaccatcggt tattacacgg acaagtagct ttttcatgga cacaaacaat
     3421 aggcgcagac tgtattttga ttgctaatga tgatgtacct acgaatgaga tacgaaagac
     3481 aacgatcaaa ttagccaagc cacaaggagt caaactagtg atcaaatcaa ttgatgactc
     3541 gattgccgcg ttgaaaagcg gggtgacaga taaatataag ttattcatcg tagtggaatc
     3601 gatcgaggat gcatataagc tggcagaagc gtatccagaa atcaaaacca tcaacttagg
     3661 agggatcaag gcaaaacctg gtactcgcag catcggaaaa gcggtaaata ttttaccaga
     3721 agaggaggaa cgcttgaaac gattaattga caaacaaata gaagtagaaa tccgtcaagt
     3781 accagcagat aaaaaagtgg atgtaagaga agttttgtaa gaaaggaaga acctcaatga
     3841 ttgtacaagc agtcttatta ggaattgttg catttgtcgc acaatcagaa tatgcactag
     3901 ggacatcgtt gctttctcgt ccgattgtga cggggctgtt tacaggaatc gtgttaggtg
     3961 atgtaaaaac tgggatcatt atgggcgcaa ctttagagtt agcatttatc ggatcgttct
     4021 cagtaggtgc ttcgattcca cctgatgttg tgaccggtgg gattttaggg acggcttttg
     4081 ctattacagc tggagccggt acagaaaccg cgcttttact aggtttgccg attgctacgt
     4141 tgacattgat attgaaaaat atttacttag ggcttttgat tccaattatg aaccataaag
     4201 cagacaatta tgcagaagaa ggaaattata aaggagtcga gcatatgcac ttgcttgccg
     4261 gtatcggtct ttccttgatg cttggtgttg tggtaatggt ttcttatatg gtgggaagca
     4321 atacgatcgg aaatctatta aatatgattc ctgacttcgt tcaaaagggg ttgtcagtag
     4381 ctacagggtt gattccagct cttggttttg cgatgcttgc tcgtttgctg atcaacaaac
     4441 aagttgcacc atattttttc ttaggttttg ctatagcttc ttacttagag attcctgtga
     4501 ccggagtagc tatttttggt gcgattttag cagttgtggt agtcaatatt atgaatgtcc
     4561 gtcaactacc acttcaaaca actgcagggg aggtagaaga agatgaagaa ttctgagatc
     4621 aaaaacagca aaataacgaa aaaagagcta aatcacgtat tttggcgttc ttttcaaatg
     4681 gagttttctt ggaattatga acgtcagatg aatatggctt atgtttatgc aatgattccg
     4741 attttgaaaa agttatatca gtcgaaagaa gaaatggcag cagctttgaa acggcatcta
     4801 gagtttttta acacgacccc acatatcgtt acactgattt taggaatcac ggctgcaatg
     4861 gaggaagaaa ataaggatga tcctgaattt gatgtgactg cgatcgatag tatcaaaaca
     4921 tctttgatgg gacctcttgc agggttagga gattctttct tttggggaac tttgcgattg
     4981 attgccacag gtgtgggtac ctctttagcc atgcaaggaa atattttagg ccctatcttg
     5041 ttcattttga ttttcaatat tccgcacatt ctgtttcgct atctagcaac aggttgggga
     5101 tatcgcttag gaacgggttt tttgaaaaag atccaggcaa acggtatgat ggaaagtttg
     5161 acattaggcg cgtctattat tggactgatg gtcgttggag caatgactgc cacgatgatc
     5221 accatcaata ttcctttaaa aatcggttca ggagaaaatg ctgtgactgt tcaaagtatt
     5281 tttgatgata tcgttccaaa tatcttatgt cttggagcat ttggcatagt gttttatcta
     5341 ttgaagaaag aagtgaaacc attaacgatc ttgatcggac tagctttctt tggaattttt
     5401 ggttcgtgga tagggatttt ttaaaaaagg agcagagaaa atgtttacga tgcaagatta
     5461 tatctatgaa gaaaaggaag tactaagtac gatcttgaaa aagaatgatt tttcgacaag
     5521 agaacatatg aaaaaaacag tgaatttact gatcttagca acgggttcat cttataacgc
     5581 ttgtttagca gcgaagccag cattggaatc ttatggtgat cttactgtcg atattcaaga
     5641 acctttttat tttttcaatt atggaaaact ttctccttca atcgatactg tgattgctgt
     5701 gtcacaaagc gggaaaagtg cttctactgt tgaagcagta aaaatgatac aaaaacaagg
     5761 gttacctgtt gttgctatca caaatgacgt gcaaagtcca cttgctttag aagctgcaca
     5821 aattattgat ttaggtgccg gggtagaatc agtaggcttc gttacaaaag gctattctgc
     5881 gactgtttta cagttactac tcttagggat aggaattgga ataagtaaaa ataaaatcag
     5941 caaaaaaata gaacaagact acatgcaaca gttgcgcaaa ataatcaacc atttaccagc
     6001 gattattcaa aaaacggaag aattttttga tggatatcaa agtttatttc gtttagcaca
     6061 gcgtttcgtc accattggat atggtccaaa ctggggaaca gctaaagaag cagagacgaa
     6121 attgactgaa acaatccgcg ttccgtcaca aggatttgag ttagaagctt atatgcacgg
     6181 cccatacctg gaggcagatg cttcgcattt gctattcttt attgaagggg aaagtgtaaa
     6241 taaggaacgt tcgcaaaagt tgcaaagata tatgagtcgg tacgttggag agactctcac
     6301 cattaccaca aaaaaagcaa gaaacgagaa aacacttgga ttagcaatag aatgtgatga
     6361 atatctgtct gtattagcgc ttgtcgttcc gtttcaacta ttcgcttata aaaccgcagt
     6421 agctaaaggt attgacttga acaaaaagat ttttgaagac tttgatacag tcttgaaaag
     6481 taaattataa gaggtggaaa tcatgctgaa attcaatgaa gaagaacaaa tcaaagcaat
     6541 aaaaggcgca ctagctttac gtccgcaagt agaagaaatc atcgataaac tttatgcgga
     6601 aaaatttgac gctgtctatt atctaggaat cggtggaacg tatgcttcct cgatgcaagc
     6661 tgtcacttat atgaatggga aaagcaactt gcctgtcttt gtacagcatg ctgcagaata
     6721 ttacactact ggaaacaaaa gattaacaaa agattctatc gttattttgt cctctgttac
     6781 cggtacaact caagaagtag tgaaagcagt agaacaaatc aagaaagtag gagcgacact
     6841 gattggattt atcgataaag cgaacagtaa gttgtctcag ctatgcgatt ttgtggtcac
     6901 ttatccagct cctggaacag aacaaatcaa attttttatg gcagcagatc gattgatgta
     6961 tttgaatgga gaattcgaag cttattcaga atactatgaa cagctcgaac gttatttacc
     7021 aacagggtta gtggaagcag agaaaaaagc agatgcattt ggcttatcat ttgcagaaaa
     7081 acatcgacgt gatagtatgc attattttat tggagcaggg aatcaatggg gagctgttta
     7141 ttcatatgcc atgtgttact gggaagaaca aagctggctt ccatcgaaat ctatccatgc
     7201 tgccgagttt cttcacggga cgttagagat cgtagaagaa acaaccccag tcacattatt
     7261 tttaggcgaa gatgaacaga gagtgttatc tgaacgtgtg gctaaacttt tgccaaaaat
     7321 ttgcagcaat tatacattga tagacacaaa agactatcca gtagaaggta tcagtgaaaa
     7381 atatcgcgga cgtgttttgt ctttcttatt gatgcatgta gtgacacaac gcatcgatgc
     7441 tcatgtggag cagttgaact gccatccatt agagatacgc cgctactatc gtcagtttga
     7501 ttattaggat agactggata aaaagaatat aaaaaataaa gagagcagcg ataactgctc
     7561 cctttatttt tttaaaatat tggcatcaat tacgttataa aacacatttt ttgcattatc
     7621 tacagttcaa cttataatct gtttaatggg acatgtgggt gccaaccatt ttttgttatg
     7681 caatagtact atttagttgt tgcatggatc cgtagtaaaa gtccaagtac aacgagttgc
     7741 gccagtttac atagcgtttt gtatccagaa tgttgactaa aacaaaagtt cctatgtcaa
     7801 ccgtcaacag agacaatctg tccgttgcag gacctgacag atttagaaat gttcaatctg
     7861 tctcttatgt attttaaatc aatactcact aggagaagtt tcctaacctc ctaatagtaa
     7921 tgctggtagt aacattgtca aaactgcttc ctcttaggaa caagcaaagc ataacgagtg
     7981 taaaaataaa atgtagacta ttgtaacata tcattgatta tttcaagtaa aaacgataaa
     8041 agaagtaaac aaacacttaa aaacaatgga aattagtccc aactttaaga aatgtatact
     8101 ataatggaaa ttatcaatta tttatttgga ggaatatcca tgaaacaaaa aatcgcagtt
     8161 cttggtcctg gatcttgggg cactgcttta gcacaaacat tggcagaaaa cgggcatgat
     8221 gtccgtattt ggggaaatgt acctgaacag attgatgaaa tcaataagta tcatacaaat
     8281 cagcattttt tacctgattt gacgatccca gaatcgatta tcggatataa agaattggca
     8341 gaagcagtgg ataacgcaga tgctatttta tttgttgttc caacaaaagc gattcgttca
     8401 gtcgcacagg aacttattgg taaaatgaat actaagccaa tcattataca tgcaagtaaa
     8461 ggactagaac aagatacgca taaacgaatc tcagaagttt tggctgaaga aattccagaa
     8521 gaaaaaagac aggcaattgt tgtattatct ggtcctagcc atgctgagga agtggcagta
     8581 catgatatca caacaattac agctgcaagt attgatcaaa aagcagcagc ctatgtacaa
     8641 aaactgttca tgaatgatta tttcaggatt tatacaaata atgatgtgat tggcgtagaa
     8701 actggtgcag cactgaaaaa tatcattgca attggtgccg gcgctatcca tggattagga
     8761 ttcggagatg atgcaaaagc agcaatcatg actcgaggat tggcagaaat cagccgtctt
     8821 ggtgtcgcga tgggtgccaa tcctctgaca tttatcggtt tgagtggtgt gggggacttg
     8881 atcgttactt gtacaagtgt tcattctcgc aattggcgtg ccggtaactt gttaggacag
     8941 ggacataagc tggaagaagt tctagaaaac atgggaatgg tcgttgaggg tgtggcaacg
     9001 acaaaagcag cagtagagct agctcaacag ctaggtgttg agatgccaat tactcaaaca
     9061 atctataatg ttttgtataa tggagaagat atcaaaaaag cagcaaaaga aatcatgtta
     9121 cgtgacggaa aaatggagaa tgaattttct cttcgttaaa tagatgatca atagttaatc
//
LOCUS       DACDSY010000116         9106 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDSY010000116 DACDSY010000000
VERSION     DACDSY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.118"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(132..2306)
                     /locus_tag="IWK44_002224"
     CDS             complement(132..2306)
                     /locus_tag="IWK44_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAP9550719.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2322..4664)
                     /locus_tag="IWK44_002225"
     CDS             complement(2322..4664)
                     /locus_tag="IWK44_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550720.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4686..7229)
                     /locus_tag="IWK44_002226"
     CDS             complement(4686..7229)
                     /locus_tag="IWK44_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAP9550721.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7239..7637)
                     /locus_tag="IWK44_002227"
     CDS             complement(7239..7637)
                     /locus_tag="IWK44_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9550722.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7718..8002)
                     /locus_tag="IWK44_002228"
     CDS             complement(7718..8002)
                     /locus_tag="IWK44_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550723.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8026..9024)
                     /locus_tag="IWK44_002229"
     CDS             complement(8026..9024)
                     /locus_tag="IWK44_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9550724.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2589 a         1964 c         1428 g         3125 t
ORIGIN      
        1 ttttttgaag ttctagtact tcttctaaat tactattgat tagttttcca tttgattctt
       61 cgttagtttt tatcagttta aaacaaccaa ataagaggaa actaacaata agtatcctaa
      121 caataacttt cttatttcgc atattgtttc tccaaatttg gtttccgaat atagacgact
      181 cgcggtggtt cctggacgtc ttcagctgtt cgatacacca caaattcgcc tgttctcaat
      241 gctcgaatgc gattcggatt gaatttaaag cggtctacgt ctcgtttcgt gcctctttct
      301 cctgtccaat caacctttcc catatcagaa taacctgctt cttgtgtcgt catttccgtt
      361 aattcaatgt cttcttctgt ccccattaaa tctgcccaat aatcaatttc tgtcgggtca
      421 tttgtcttcc caataaagaa cgtattcaca ttcccaatca actgttttcg aaggacagga
      481 tcaattctat caatgtccgc aagcgtttga ggggcatacg ccgcatgcac cccagcgcct
      541 cgtcccttat tgactaagtc tataaggtta tcatttagat aagaagctgg ttcgtcaaat
      601 attactagga ctttcgagcg attggctgtt tctcctgctt gtttatacac ttctgacgca
      661 aagaaattaa tatctcccac aagcatttga gccaatgtcc gaataaactc actataaatc
      721 aatccgttta acgagatata aacaatcttt ccttctttaa caacttcttg aatatctaat
      781 tctctttttc ctccagaagt atcaaagagt tcaccaagct cactatccaa taacatattg
      841 atattggtct gtaaattctc agaagtcgca tacaaataaa acgggttctt cttatgttcg
      901 taacgaacaa acaaccgttt aaacaacgtt tgctcgattt ctgtcatgag agaaatgttt
      961 tcttttaata atagataata cgtttctaag tcaacggttt tacgatcatg ttcaacgatt
     1021 cgctcttcac ttaactgttc cacatcggca aaatcacgtt ctgttttgtc catcgcttca
     1081 ttctttttcg tttttttctt cgtggttttc ttcttttcta cgaccacttt ttctttaaat
     1141 aaatctggat ttttcaactg aatacgatcc gcaaacaaat gtaagacgtt tcgtggcaat
     1201 aaataatgtt gtaaccaaac tagatttctt tcaattttat ggctcttttg gaactcgtct
     1261 aaaagctgaa tcgttacctg taataacgct ttggctgctg tcgaataata cacgctttct
     1321 gtttctgcta gattggttaa tttatctcga atggcaatat cattgccatt ttttacagga
     1381 ttataacgca aatcgccacc gtccacaaag acttcaatcg ggcgattgta tgcctctgcc
     1441 acttctttta acgcttgtaa agtctctcct gctcctttcc catcgactaa gataacgggg
     1501 atacgaaact tacacgcatg ttctgctagt aattggatca ccgtcgtttt tcctgaacct
     1561 gtagtcgctg gaataatcac atgttggttc aattcactaa agttcatcac ataagggagc
     1621 ccatactcat ctgttcctaa tagaatcact ttggctaatg aagccagttg tttattccgc
     1681 aaagcaacgt cttttatgac aattgctacc tggtacgctt gctgatactc tgctgtatat
     1741 ttttgggact cctgaaattt attcttccga cgttccttgt attctttact atttagcaaa
     1801 gctgtttgcc gttctttctt tgaaacaact tttctttttt caataaaaga cgcatacgca
     1861 atcaaacaac ttcctgcacc tgtcccacct aaaaggaggg caaaaatcgt ttgtccatta
     1921 aactgcaacg catgagaaaa caagtttaat ggaaataata agtaacacga caaataccct
     1981 ctaggaagaa caaaaataaa acaaaataga tttaatagcg taaaacctaa cgcaaaaata
     2041 aaaaaaagag agcttcttcg ttttaagaag ctctcatact caaagtcata aatatgtgca
     2101 cgtgtctctt ttttatctat cataaagaca atcgctaaag gaatactgcc tacaataaaa
     2161 aatggatata gacaaacacc taataaagtg acgataaaat aaagttgttg aaaccgactt
     2221 tttggcgttt ctttttcttg ttttcttttg cgatcgacag aaaacatttg ctcaaaatgt
     2281 gtcggtcgtt ttggtgcatt catcatacta tcgatttcct cctatcgttt tcgttgtttc
     2341 atttctcttc gataaatcgg ttgtttttct cgttttcgtt tcatttttgc ttgagttgct
     2401 cgaaactcac tccgtgtctt cggtgcttgt tttggtgatt ttgtttgttt catggttgag
     2461 tatgctttag atacggttga aggcgctttg gtttgtgctg ttactcgatt gactgctgac
     2521 atatgttccc gaaccccctc tggacgtcga acagatttcg gtgtggtaga acctgttgct
     2581 cgtggtacat tcccttttct tagtggtttt tgtaccgcat gtttttgtcc tgcaactgtc
     2641 cgggttttca tcgaacttgt ttttgttacg ggagtaagtg cctccttccg acgacgactt
     2701 gtttgtccct gacgacgttt tcgatcgttc aattctgttc taggcgtatg cttctggcca
     2761 taagacttgt gtttttgctt tggattcgct aatgacacca ccattgcaga accctgattg
     2821 cgatacggac tacgagacgc accttttcta cttctttctc ttagactagg attgccttgc
     2881 aattctcgtt gtaataaatc ggctgttttt cttgcctgtt tctccattgc tgttcgttgc
     2941 atacgttctt gactttgctc ttctgcttgt ttcgcttgat catgcaagtc tcttgctttt
     3001 tgtgccatac gttccatacg atttggtgct ttatgacgag gttgttgtgc ggattcacgc
     3061 cgaaaagcac ttcgtgtggc ttgtttcgcc gtttgtggca tacgaacgtc tgagccaccc
     3121 ccacgttttc gttgacgttc ttgtctcttc cgtgcgtttt ctttttcttt ttgtttctgt
     3181 gcttttctcg cttctttgcg ttctttcaac cgttgtttgg cttcccttct caattttgct
     3241 tctgccccaa atggatttcc cgcaaaagct aacgctcctc gaacacccat tcttccagta
     3301 aatagactgc ctaaaaagcc tcggaacaaa taaattccca acggcgcaaa caaataaaca
     3361 atgatcaaga gaattttttc ccatggatcg tcttgtgtac tacttaattg gaacccgtaa
     3421 ctaagaaaag aagaaaaaag gatacatgca aaacctgtca ttccttttaa aaacgtcaaa
     3481 ataccaaaaa ctttggcata gttaccaaac acattcattg tgacaagaaa caatcctaaa
     3541 aaaagtaaga acggcaacat cggtactaac aatagttgca tagttccaat aatcaatcga
     3601 aacatactta agacaaaata aacaacggtt tgaatcagat tcacgacaaa ataaaacaag
     3661 gcaaatgctg tatttttcca actattgtaa tattgtatgt tcttgttttt cagtgtatct
     3721 gcttcatact ctgtgacttt gtcatgaatt ttctccccta tttttgtacc taaatcattg
     3781 tccaataaaa gattgattcg atcatattct tttccagcat atttgacttt cttctcacga
     3841 attgtgtcta cattggttgt gccataattt aacaacagat atggttcata aacattacta
     3901 taaaagaccc gagccgccat caatttgcct gccgtttcca cttttttatc cgctgttacc
     3961 tcttctgttt cttttgtttc ataatttgtt tcttcttcag gtgttaacac tggattgacc
     4021 ttcacgaacg ccgtttccac tgtagaatct aaagaaaaaa gaacatcaaa aagattctta
     4081 tttgtcccag aatcttttaa tacgcttaac ccagtaaaaa taaggatcgt cataagaaag
     4141 acacggagtg cttgtttcat tcgagcctct ccagcatatt tgattgccat cataattgcc
     4201 gaaaaactga ttccaatagt tcctgcaata cttaacatat tgtccgcaat tttttgtgtt
     4261 aagttttgaa tgggttctcg aatggcggaa ataatatcta attcaaagag aaattcaacc
     4321 attgaagcat tgaattctcc taacattttt acgccactcc aaacaaaatt tttcatcact
     4381 cctgcaacat tgtacgatag ctttttttta aggttcattg actcatagtc attggtcatc
     4441 aattcaaacg aattcttttt gtaattttta tacaattgat ccacatcagt cgttacttct
     4501 tttacttcta ctttatcgtt ggcattctct ccttcagaac gatcggtttc agtgacaccc
     4561 gcaccatcat tcatttgatc ggcagaaaca gtagcccctt gtagacagaa aaaaagaccc
     4621 cctaataaaa ggagcgtcca ccatttcaga gatttttttc gcaaaaaaac cacctctttt
     4681 ttgttttatc gttgtttttc attttctaac attgcttgaa ttttttcatc ttgattttca
     4741 gtagaagaaa cagccaataa ccaactatca aacaacacat caatggcaat cggttgattt
     4801 cttcccaaat gatcctggaa taaacaggtt cctgtttgta attgttccat catttctaaa
     4861 ttggctgaat tcacttccat atcaaagaag gacaatagtt tctctcgtgc ttctttttgt
     4921 ttcggacgaa acgcaaattt gtaagaaatt aactcttttt tatcttcttg atcgtaatct
     4981 ttaaacgctt gcacaacaag gtaaatatcc gtattattcg cacgtccttt tcgcaagcta
     5041 tcatcaatca aatagcttcc ttgtgcggta tcttcatacc cttttgcctc gtcataaata
     5101 atcgcagcgt cttcttcttt gtttgtagaa aagatatacg taaacttcat aatgacttcc
     5161 ataattgcca ttcctgcaag ttgttcgcta tttaatcgac ccgaatcaat ctctttttgt
     5221 gtcggaatgc gtattccttg tgtccctaac acgttgattt gtgaatcaaa acgtatcggc
     5281 gtactgtgct cattcccaat gagaatcttt cctaaaccaa cgctatgccc taaaatcaat
     5341 ttagctagtt ctttgtcact ttttcgaatt tcttcgataa cttgacttaa cgaatgctta
     5401 cctggcatgg tgtaaactac atcgatggcg tcttggatca ccgttttctt gtggctcgct
     5461 gtttctcgat tggtatctac ttctcctaac attcctaaca catttttagc ggtttgaatt
     5521 gcctcttctt ttggtaaaaa caatagagga tcaagcatcc ctcgacagct ttcatctgaa
     5581 gataacgaaa taaaattaat tcgttcatac atagccacaa actctggtat gtgtccgaat
     5641 ttttcaagtg cctttaaaaa gaacttttgg gtttcatttt tcggatcaat ataaagaatt
     5701 ttttgaccta aaaacatcag ccataaaaaa atatatttta caaggaccga ttttccttta
     5761 cctggtggac caataatcaa cgtatttcca ttggtatgtt ccgcgccttc aatacttttc
     5821 tttgttaaat tgggataata ccatactagt ttactagata gcgctaacgc ttctttcgca
     5881 gatttaaact tttttgcaat aacaatccgt cctaaaggaa atcctttctt atttcctact
     5941 tctttcttta agtccaagcc ataatctgcc acaaaacctg tcgtagcgac ctgctcgtaa
     6001 ctacgaaaac gttgcgtact tccaattaag cattgattaa acaacgttag ctgatccgct
     6061 accgatcgat aaaatttgaa atctgtagac tcaatcgcaa attcaatatc tttgactcgt
     6121 tcttccaatt cttcttttga agaagcagat acaaccagcg tgacggtaga ataacacaat
     6181 cgtttttctt tgcgcttcag cttatctttt acatccatta accgttctgt accaaaattt
     6241 aatacatcgt ctgaatctaa aatgccgtca acggtctcat attctttttc ttgttcataa
     6301 atccgtccag ccattttatt aatttttcgt tcatcagatt ttgaatgctc aaatcgaagt
     6361 cgaatatgcg tatcaattgg aaaagataag ctatcctgaa gattttgaat aaagctactc
     6421 ccatacatgg ctaaaggagc gtccgtttga cttaaaaacg tcgaataatg cgtttgattc
     6481 aactgttcaa tcgcaatgta tcctgcttca ttcgaaatga cgccctcgca aatttccatt
     6541 ggcgtaagct gtctttcagg caatttttct tctgatcgat ggaaaaaata ataataaatt
     6601 tgtccaatct cttgggcaga taagcgctct atcggcttat aattggacaa ttccgtatag
     6661 agctcctttt ctaatgtttc atacgttggt aacacactac ttataggaac tcgttgtccc
     6721 gtaatacgat agataaactt tttaaaaaca tgcttaagca attgaaaata ttcgatcgga
     6781 tcagacactt tcatttctgt agtgaaacgc acaaacagat acgttcgata ctcgtttaat
     6841 tgcacttcgt cttttaaaat ctttcctgcc tgttcaaagt acactttccc aagatctgcg
     6901 cattctccct ttaccaaatc tcgaatggtt tcttcaatgt gcgtatctag gttaaattga
     6961 cgtggaatat tgataaaatg atagtcatat tcatcatgtc caagtatgcc ttcgccatct
     7021 tgaagatagt tcttgaaaaa atcaatgtca tttaaaggaa atacttggga agaaagttga
     7081 tagcctaccc acacatcatc atttttggtc aaaacaaaat ttcggataat ggcgtctatc
     7141 ggaaattcta atcgaggaag cttcgttttt ttctttttga actgccaaat cccgataata
     7201 aagacgacca aaagaaacaa aaacgccatt tactcgactc actccttcca tttgtgtagg
     7261 gaatacgtca ctttttcatg gaaaaacggc acatacattt catgttcaaa gcgttgtttc
     7321 cgtttctgat aataggacgt tcgcccaatt aaatactcga aaaagtgttt attgtcccat
     7381 ttcattgaaa acaatgtcca aacgagaaca ccaatcccaa aaatcataat caaccaggac
     7441 tttcgaaaca gtgtctgcaa gaaactaatt tgattcacat acgcaatcaa aaataggatc
     7501 aaaagaatga agagaaatac cccacctatg actaattgtt ccatgcgaat gccattagaa
     7561 atccagggaa ttttttgtcc tttaatcccg taaattttgt gagggtgctt aaactccttt
     7621 gtatagttgt attccatgtt cactctacct ccttttacaa aaaaagaagc gaccagaagt
     7681 tctaatcttc tgatcgcttt tgcagtttgt ctttttctta tgataattgt atccagggca
     7741 caatcatttg cgttaccccc acgttctctt cactatcctt aataaagata atgttcttta
     7801 gccattcacc aatatcaacg gcattcgata acaataagac gagcattatc aaaataaaga
     7861 cgccaataat ggcaaacgct gcccgcgcaa agccttgggt cgcataagca ccaaccatta
     7921 gaacaatcgc aacgattgcc aatagccaaa tgacttgtcc agatacatca tcaaataacg
     7981 atttaagatc aaaaagtttc atttccattc tcctctaagt tcattttatt catttaacgt
     8041 agtaacaaaa taactatcgc cacttgtttt tacttccatt gaaaagtttg attggaactc
     8101 gtttcctttc tcaaaataga aacgatactt cccttgcaaa aagtagtttc cttctttatt
     8161 ttttactgca tgatccagtt gtacattgat caattcgccg ccttctaacc ctttttcgtt
     8221 agaaatcaat gccaattttt catcattttt gacatataac tcaaaaaacc gtttcacaaa
     8281 tgcttccagc ttgatttctt cgctttcagt gaccgcttgc ccagaagaat aaaatcgatt
     8341 ttcctgatag ttcacagaat tggtattctt actctcagaa agattcaaaa attgaacgcc
     8401 atcaagtact tttaaaacat tgttttgata actgactgga aaagaaagtt gactgctttt
     8461 ccaatgttct ccttctgtaa aagtaacctc ataaaatagg cgatacattg cttgtgtcaa
     8521 atcgatttct tctagtttaa taaagcgaac atctttcgct actcgattgg tagccgtcgt
     8581 tacaaagcct agatcagacg cttccaatcc ttgtgctaaa taatgattga cggaagcctc
     8641 ccattcgttt cgtttttctg gcgaataagt aaataacctt tcaataaaat gttgtccctc
     8701 gtacttcaat tgttccgtag aagacaaggt ttggttgact tcagttaaca atgtttctgg
     8761 attgacttgt tttttcactg ctaaacgcgt taatgtatcc actcgtccaa agtttaggag
     8821 gagcacgaca cccattaaca gaaacaaaca aaaaaaagcg cctgaaaata cactcatggc
     8881 acttttttgc gaaaattgga tcattcgatc tgacggttct gttttctttt tttggaacaa
     8941 aaaacgtttc ttttgtttct tttccttctg ttgtttcggt ggcttagttt ggaacgcttg
     9001 tggattaaat tttgcattac tcatgagaaa tgccataccg ttctaacaat aatttcggta
     9061 ttttgtccac aatcactact tcgtggacaa ccacacttgc cccctc
//
LOCUS       DACDSY010000117         9066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDSY010000117 DACDSY010000000
VERSION     DACDSY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.119"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(42..302)
                     /locus_tag="IWK44_002230"
     CDS             complement(42..302)
                     /locus_tag="IWK44_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550725.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            377..580
                     /locus_tag="IWK44_002231"
     CDS             377..580
                     /locus_tag="IWK44_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP9550726.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(721..1206)
                     /gene="ssb"
                     /locus_tag="IWK44_002232"
     CDS             complement(721..1206)
                     /gene="ssb"
                     /locus_tag="IWK44_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9550727.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(1209..2108)
                     /locus_tag="IWK44_002233"
     CDS             complement(1209..2108)
                     /locus_tag="IWK44_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9550728.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(2121..2714)
                     /locus_tag="IWK44_002234"
     CDS             complement(2121..2714)
                     /locus_tag="IWK44_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP9550729.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(2955..5159)
                     /gene="topB"
                     /locus_tag="IWK44_002235"
     CDS             complement(2955..5159)
                     /gene="topB"
                     /locus_tag="IWK44_002235"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9550730.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(5170..6630)
                     /locus_tag="IWK44_002236"
     CDS             complement(5170..6630)
                     /locus_tag="IWK44_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9550731.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(6691..9063)
                     /locus_tag="IWK44_002237"
     CDS             complement(6691..9063)
                     /locus_tag="IWK44_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550732.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2217 a         1890 c         1517 g         3442 t
ORIGIN      
        1 aaaaagagct gcaatcctca tttaggactg cagctcttct tttatatgcc catcgatcgc
       61 gtaaaatgat cggggtttct aatttgcatc gattcaatga ctaattgatt ccgtttattg
      121 aaatgacctg tgacggcaat attgtatttt ccatcatcca taattaacaa tttatcgata
      181 atttcaatat ctgcaactac acaattaatc gattcatgaa ttgtttgtaa agtaaagcga
      241 accaataatg gttgtgtttt tattactcgg attttatcaa gcaaaccaac atatttgttc
      301 atcacgatca cctcgctaaa attatagcaa aaacagctaa aattgagaat ttttattcga
      361 ttttagatat gctataatga tcaagacagt aaagtttgtg acggtggcta tccgattgaa
      421 agtgaggtgg tgcgtatgtg cgtacttcca aaatcttaca gaaaggagaa gccttttgcc
      481 tgttgaaacg gcgctaggat taatgattag tttcggttct ttgatcgcaa caatcatttt
      541 tggaatctta gcagttgtaa acaacaatca aaaaaaataa ccgtcagctt tagccggtcg
      601 acgattattt ttcaaaaata atattaagct ggtcaccgtc ttaaacggtg ctgttagggg
      661 agttgttagc gcaactcctc ttttcgtatc cattataaca actttattga tcttttacaa
      721 tcagaatggt aaatcatcat cactaatatc gattgattgt ccgccaaatg gattttgtga
      781 agcgttcaat ccattgttgt taggcgtagc tacatcccgt tgttcaaagg tggtagggct
      841 tggactgctg gacgattgat cagcgtgcgc acggctttct gtcgctgctt tactttctaa
      901 tagttggaaa ttatcggcca caacttctgt cacatacaca cgttgtcctt gttggttatc
      961 gtaggttcgg gtttgaatcc gtccaacaac gccgatcaaa ctgccctttt tagctaatgt
     1021 cgccatcgtt tctgctggtt tgcgccaaat cacacagtta ataaaatcag cttctcgatt
     1081 gccgttttga ttcgtgaaat ttcggttgac agctagggta aaagtcgcta ctgcagtacc
     1141 actcgcggta taacgtaaat ctggatcttt ggttaagcgt cctactaaag ttacattatt
     1201 gatcatcttt atctctccat ttcttgattc atttgttgtg atcttttaaa ctgccgtttt
     1261 ctttcttgat tttcaatttt gatcacactt ttcattaggt gatcaatttc gttgactgta
     1321 tctgtaaatg aaatggaatt cgcaaacgtg ttaactgagc gatatttttc ccactgcttt
     1381 tccaaatagt catttcctct gatcttatcc atttctcttt cataataaaa ggacaacggt
     1441 tttgtatccc cacgttttgt aaatgtattt cgaacacttt tgtataactc tttcgtatcg
     1501 atattttcta gtttcggaat ggtaaataag tcgtagtaat cttttgctct cgaattatct
     1561 gtgccaaagc tgaacgtagc acttagcttt tctgcaataa tttgttctgt tggataagct
     1621 ggaatctcca cttttttatc ttcaaacatt aattggtgat agtgtgtatc tacttcagga
     1681 taaatagaat ctcctgttgt cacgtccatt ttaaacggta cacgtacttt ctctaaatta
     1741 gccaccaatt taatttgaaa tccaggataa aaatcggctt ctcttgtttc cttcattcct
     1801 tgaatagaga aaacaatgcc ttcttttgtg ggttttctag ttaaatcatt aaaaatgctc
     1861 ttcaattttt cttcagttaa tttgctattt ctaaatgtcg tatcgatatc aattgttgtc
     1921 cttctatcga ttccatattt tgatcctaat aaaaagccgc ctttcaaaat gaagtcattt
     1981 ttgtactttg attcagaaat ttgttcaatt agacgatcca tcataaaatg ccgttgcact
     2041 aactgtgcat ttaatcctgt actctttgct aaatttcttg tttttgctgc aaattgtgtc
     2101 ggtgtcatct ttttcctcca tcaatataag ctttgcatgt ggtatttcat ttctttttct
     2161 acatgtagtt ttgtcatgta atctcttaat ttacttggat ctctcaatgg gtcttccatg
     2221 tactctttta atgcttctaa tttcatttca tattcaaatt catatctcgg attccaaata
     2281 tcacaaagtg tacgctcttt atcatacata gtaataggat ttccttgaaa ggaagttccc
     2341 tctacttttc ctagttcata aacttctttc ttcacgaaat gaaattgtaa ctgctgctct
     2401 tttgtaattg aacgatcaac gttatagcca tggggaaaag ttaagtgaat gaatttcggc
     2461 atattcgtag aaagctggta aatatctaaa gtcgtttcat gagagaagat tcccctggaa
     2521 aatcgttgct gaagccaaaa taaatcatct attccatgac catcctctac ataataacca
     2581 cgatcaactt tgatcaattt tccttgattc accaatcttt gtacagtggt tgctgcgatt
     2641 ccaaatttcc tggcttcctt catagataaa tttccatcgc gaatagccaa caattctttc
     2701 agtttatctt gcatttactt cccctccctt cgttacaaat acactacatc aaatatatca
     2761 tagtagttta tttgtaacaa gattaacgtc tatcgatcta attcgatttc tttttgaatg
     2821 cgctgctggt tctttaattg ttgcagtctt cttttatttg cacgtaattt ctcttgttct
     2881 aaacgaattt ttgctttttg attttcgatt tcttgttgtt tgtgtgttgt atttcgcata
     2941 ggataacccc ttcattattt tgtagtgcgg cgtgttcttt tcttatagtt cgtttttttc
     3001 ttttcagaaa attcaaagcc tgttgtaaag tcttctttta aaacgatcgt tgcttcaaac
     3061 gaacctttct gaccttttag tcctctgact gattggcttg ttcggccgtt ttgaatgatc
     3121 tctttcagtt gtgtggctgt gagtttcttt ttggcaatcg ttgtccataa tttaaaatca
     3181 caggcttcag acgtacatgc cgccacattt tgataaagca agacttcttg ttcccgacat
     3241 tttggacaaa cacctaaatg acgcgttgct tcctgttttt cactggctgc tttcacctct
     3301 tgtaacgttg atccaaaatc tgttgactgg atttgtcccg gtacgacttc caacaagtga
     3361 gagacaaatt tttggatatt agctaagaaa gtagcttgtg ttcctttctg ctcaccaatc
     3421 tttaataagt aactttccca tttggcggtc atttcggcac ttgttagcag cggctctttt
     3481 tccacggctt ggcaaagtaa aatcccttta tttgtcacca caagcttatt tttttctacc
     3541 ttgatatatt ccttttgttt caaagtttca ataatatttg ctcgtgttgc ttctgtaccg
     3601 attccttcta cttctttcaa tatggattgt gcttcttcgc tatccacggt ttttccagct
     3661 gttttcatcg ctgtgatcaa cgtaccttcg gtataaggtt gtggtggctt cgtttctttt
     3721 tcaaggactt gtagatctac cgtcaccgct tctccttttg tcactttagg aagtagcggt
     3781 tcttcttcct tttcttttct gtcgctctct ttgccaaaca gctttttcca acctagatct
     3841 actggtactt ttccgactgt ctttagctga agctgctgaa cttgcgtcaa gatcgttgtt
     3901 tcttcatagg tatacttttc cgcaaacatg gccaccgttg tttttaacac ttgttcataa
     3961 atagcctgct gcagtggact taattgttca aaagccgctg ccgtcatgac cgtctttgta
     4021 ggaatgatcg cgtaatgttc ctggactttc ttattattca catatcgact attcggtgta
     4081 tgaataggag taggaatggc ttcggccttt aaaaagtgtt tgtattcgtc taagtgatcc
     4141 aacagatacg catactcccc ttctgtaata taaggggtat ctgttcgtgg gtagcttaga
     4201 tatttccctt catacaaccc ttgcatggct tccaaagtat ctttggcact agctttcatg
     4261 agctgattca ttttagattg aaggctggat agagaaaaaa gacgtggact tgccgcactt
     4321 tttttctcgg tttcaacttg gtggatcgta cctggctgtt ttcccaattg aacccccttt
     4381 tcttttaaat aatcttcaaa tgcttcttgc gtcgcttggg tttcattcgg atcaagcttc
     4441 gcatcaaatg cgccattttg agcgattact tgggcttttc cttcaaaata tggttctttt
     4501 tggaagtttc taatcttttc ctgtaattga taaatcatgt aaagcgttgg ggtttggact
     4561 ctgcctaagg aaaaacttcc tggtatgcct ttttgctgca agagtaaact atacagcggg
     4621 ctaccgttca tgccgatcaa ccaatcggcg atctggcgtg cttgtgcttc ttcaaatttt
     4681 gggtaggtct cttctgcatc tcgtaactgc tggaatccct gataaatggc ttctttttct
     4741 aaggagttga tccaaagtct tttaaatatc tttccatgat cggcgcctgc ttgttgaatg
     4801 atcgtccaag ctatcagttc cccttcacga tcgctatctg tcgcaataat aatggtgtcg
     4861 gccgattgaa gttgctgttt caccaccttg aattgctttt tcttatcttt aggaacatga
     4921 tagtgataat gtgtagggaa aataggcaaa tgctctaatg accattgttt ccaattttca
     4981 tcgtacatat cgggcgaatc taactcgact aaatgaccaa aaccatacgt gatcacagtt
     5041 tctcctgaaa acaatcgatc ttggatctca aaatacccat cttttcgggt agctttagaa
     5101 aagctatcgg cataagcctt cgcttgtgac ggtttttctg ctaaaataac ggttttcatc
     5161 atgacgattt cactcccttt cttgctgtct accttgttga aacaacatgg cacgtttttg
     5221 ctttctgatc tcttgttggt agatttcatg cttcaattga tcattccaat ccttttttac
     5281 ttcaggtaca aattcatgtt catgattttt atagcctttg tagtattgtt ctgctttata
     5341 agagaaaaga atctgagcat taatatcccc tttttcttga taaaaacgat caaaatcgta
     5401 cgtaccatcc acgcgttcac aagcttttaa ttcttttgcg acttctctac aaatatcttt
     5461 caaattaata gcttctattg aatcgttaac agaaagctgc tgaccaaaag acgcatttac
     5521 tttgtgagcg gaaaccaatt ggttcgttaa ttgcagattg ttttctgtct tgtgtatggc
     5581 cataatagct tctggtgtga tcttttcttc ttttgcgact tcttcataaa tgactttgta
     5641 ttcttttggc acttgccaat cattagcgat tcctcgttca caacgtactt tctgattcgt
     5701 aaatggatca actgcgccca ttaattgttc tttttcgtag aaaatatgtc ctgcacggtc
     5761 attatccaca caaatctcga tggattgagg aaaggcttgt ttctttctca attcagatac
     5821 tgcctcacca aaataatgac tgatcacatg gtgttttaac ccttccatcg aaactaacca
     5881 cgtatcgttt aactgatcac gatttagtgc agcatagcta agtaaatcaa tactactctc
     5941 aaaaaatttt aactgcttag ggtcaccgat ctttaggtta aagccatgat tcgctgtcga
     6001 atttttatct atatgcttat atgtgcctct ctcaccaaaa cgttcataat caatgaacgt
     6061 tccttgataa ctcgccccta ctgtctcatt tgtctgtgga tctttccata aaaaggcgac
     6121 cgcatgatct aaacgatcgt ctcgaactaa gaaattttgc cgttcatgtt ttggctttag
     6181 ttcggcaatc aacccttctt tttctgcata atcaatcagc cactctggta gttttcgttc
     6241 ttccttcaga taattccttc ctttttctgt gagtggttga taatcaacgg ggtgatcttt
     6301 gtacttttct gtctcaaaat gaagattttc cactcgttct gttttgtctt cattctttag
     6361 aataaaatca gtggctgctt taaattgttg cttagggtct tggatctctt tgtctatgaa
     6421 aaaaagttta gctaattcaa taatgtcgcc actaatattt tgactgttcc aataaaaacg
     6481 ttgttttctt cgatcaaaaa caaatgaatc atggtcttct aagcgatagt ctcgtccttt
     6541 attgactaca gccattccat tgtttcgagc aaaatccact atatcgatct gtttacatcg
     6601 atcaataaga ctcatttttt tctcggtcat tttaaaacct cccaaaagaa aaaagactat
     6661 ccgtttttca gatagtcttt tttacttgtt ttatacacgt tctttttgtg acaattgttg
     6721 ttcataatag atttcatttt ttcgttttgc cacttctgac actgtctcaa gtgaaaatcg
     6781 gaatgaattt cctagcgcaa attcttctga aggtaacgcc gtttggatct cttttccata
     6841 actttttcct tttaaaatta cttgattatg ctgtggatct tcaataaacg ccgcatcctt
     6901 caaatgtaac gttcgacccg tatcttcttc cattactttc ccgtctttct tgtcgatcca
     6961 aagtgaacca acaggaacaa tattcttttg ttctgccgtt tctacagtat ttttatgctc
     7021 ctgcttcact tctgctggtt ggatctctgg ttctctttct ctgtattgat tgatttcatt
     7081 agaaatggtt tgaatgaatt catttgcgac acggctgaca tcattcatga ttttcccacg
     7141 ttccgcagct tccttatcat ccaacgtctg accattcttc gtccatgctg caatataagg
     7201 aatagctttc tccgaagtgt ccattccaaa atgtttgcac acaatataag aagtcatttc
     7261 tgcctgcaat tctttagttg cggtatctaa ttctgaaaat ttgtgcattc ttgcatgggc
     7321 taattcatga atacttgtag ctaaatgctg cgtaggagta tttcgagtat tcatgacgat
     7381 ttcttcttcg ccagtaaatt cgttacgaac atatgccccc cgtgcttgtc ctaattctct
     7441 ttgatagact tcactttctg tcatatctcg aatttgtatt ccaatattgt tggctaccgc
     7501 ttgaatccca gcttgaagtt ctgactgtcc ttttttatcc aattgaaaat caaagacacg
     7561 gttcggaaag attttcggat agtcttcagg ttttaactgt gtttgtgtga tatcaaacac
     7621 tttttcaatc gtgtagtaag gttttttgat cacttcgatt tgaccacttt cgattcgttt
     7681 tttatcttct ggtgtagctt ctgtttctcg aacaaagcca cgtcctttct cataaaatcc
     7741 tttgaccgtt tttctgacaa agatcttgtt cccttgttcc ccttttttga tatagattcc
     7801 ttcttttttc aacgcagcac gacctaaaca agccgttgca cctggaaatt gttcctcaat
     7861 caataattga tttctaaagg atcgttctgg aaaacgagac ataaaattca aaagttcttt
     7921 cacgtcttca ggagaatggg tatgcttttt caccgcatcc agtgcctgtt gcgaaaggga
     7981 tttgacttct tgcttaatct cttctgccgt tcgtttttga tagccctttt gttttgtttt
     8041 tgtttctttc gactgcattt gttctttcga aacaggttca tttttgaccg tttgtttttg
     8101 ttccttttca ggtacttgtt cgcacaattt tgctaactct aaatagatgg gttgattact
     8161 gttgacgcca tcccctagat ccattcggat ctgatcgact acttctccgt tcttcacttc
     8221 ttcaaaatag aatttataat aacctgcttc tgcggcgtat tggttatcca acgttttgag
     8281 ttgatcgatc aattctaatg ttacgatctc cccaccataa cttttcaatc ctttttctgg
     8341 atcattttcg ttgaattcta ctcgatagaa atgatcgttt tctttctcct tcaaagaatc
     8401 agttggcgca gcctcttcaa gtaattcacg aacagctgct ttttcctctt ccgacgtggc
     8461 ttggtatttt tttgattcct caaatccccc gttttcattc agttctaaag caacttttcg
     8521 atagtctttc aaaaaataaa tggttggatt ttttttccat tgcgtcactc gtccgctgtc
     8581 aataattgct tgtacttcag gaagaagatt ttcttttcct ttttctgatc tttctttcag
     8641 ctgttctaat gtttccaatt tccgttggta ttttcggatc gtagcggctg aaaaagtcga
     8701 ttcacctttt tcaaagcgtt cgatttcttc tttgattctt ggaatatttt cgattgtcat
     8761 atccaggccg ccgtacttgt tatagcccat ttcctttagc tcttcttgtc tttcaagttt
     8821 ttctacctgt ttttcttgtt gcttgatctc ttctctcaga ctatgcactt tgttttctaa
     8881 acgttcttgt tgcctaaacc aagaatgccc atttctctta tcgttcattg gctgaccgtt
     8941 ggtctttctc tggtgttcaa acatcttctc aatttgttcg tttaattcat tttgcaatcg
     9001 tactaatttt cttttggatt ttcccaagcg actattgtct agtggttctt tttcttctgc
     9061 catgtc
//
LOCUS       DACDSY010000118         8991 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDSY010000118 DACDSY010000000
VERSION     DACDSY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.120"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..550
                     /locus_tag="IWK44_002238"
     CDS             <1..550
                     /locus_tag="IWK44_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9550733.1"
                     /translation="LQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRGVFL
                     KFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITINLL
                     EEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELKIEF
                     SKQNHYFFVEMYLASRKSKSAY"
     gene            872..1099
                     /locus_tag="IWK44_002239"
     CDS             872..1099
                     /locus_tag="IWK44_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9550734.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            1222..1548
                     /locus_tag="IWK44_002240"
     CDS             1222..1548
                     /locus_tag="IWK44_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9550735.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            1616..2329
                     /locus_tag="IWK44_002241"
     CDS             1616..2329
                     /locus_tag="IWK44_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9550736.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            2639..3490
                     /locus_tag="IWK44_002242"
     CDS             2639..3490
                     /locus_tag="IWK44_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9550737.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            3611..5476
                     /locus_tag="IWK44_002243"
     CDS             3611..5476
                     /locus_tag="IWK44_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9550738.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            5524..6933
                     /locus_tag="IWK44_002244"
     CDS             5524..6933
                     /locus_tag="IWK44_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP9550739.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            7041..7742
                     /locus_tag="IWK44_002245"
     CDS             7041..7742
                     /locus_tag="IWK44_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9550740.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            7901..8380
                     /locus_tag="IWK44_002246"
                     /pseudo
     CDS             7901..8380
                     /locus_tag="IWK44_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         3037 a         1479 c         1975 g         2500 t
ORIGIN      
        1 tcttcaggaa aataaaaaag aaacgttgga atttttagaa atattagaag atcattctgc
       61 agctattttt gaaaaaccgc aaatacaaca gctaacaaat attgtatccc cagctgtcag
      121 gggtgtcttt ttaaaatttc tagaaaaagc agaatcggag aaaatcccga tccaagtagt
      181 tattcctgtc gaagtgaaaa caatcccgat tgatctggtt gtttttattc gctgtttatc
      241 tatttttatt tctaacgcat tcgatcatcg tgtagccgat cagagtccga ttacgatcaa
      301 tctgctggaa gaaaaagagg gatcacgttt tagtatagcg aacaaggcaa atccaacgaa
      361 tttgacgcta tcggagatgg tcaaaagagg aaaaacctct aaaaaaaatg gcgggttagg
      421 tctatacagt gcgaaaaaaa tgttggatgt ttacgaaaat gcagaactaa aaatcgaatt
      481 ttcaaaacag aatcactatt tttttgtaga aatgtatctg gcatctagaa agagcaaatc
      541 agcctactga cttactgtca aaggataagt ttgaggaaag tctacacgga gatagagaca
      601 acaacacagc atattcaaaa gaaacagaag cgtgatgacc gttgtcaccc ttctgtttct
      661 tttgctatgt agttaaacaa agtagttgac acttccgcta cttagtatta gtacgaaagt
      721 atacttcatg atactaagta gataagaagt gaaaagcgga acccacgatc gtcaaaatgc
      781 taactgcttt gcttacacca gataaaggga agagcaatat cgcttatttt aggcgtggag
      841 aagcaagcaa atccctacga tcacaaaaaa aatgctgacc cagcagttaa gagagttaga
      901 acaagatgaa agtatccatc gaaaagccta taaccaagtc ccgcctaaag tagagtactc
      961 gctcaccgaa gaaggaaaat cattaagaga gattctagta gcgttgtctg tttgggaaga
     1021 agaacgtatc gaaaaacaaa aagcctcaag ggaagaagac acgctactgc atacacatgg
     1081 tggctaccta aattattgat tttctatgac atttatttta aataaaagaa aacaggagtt
     1141 tttttaaaag catcatctat tttttatgta aaaaaatggt atgattaatc aagtaagaag
     1201 tgattggaag aaggcaagca aatgaatagt atggaaaaag tcaaccagtt tagagatgaa
     1261 cgtaattggc gacaatttca taacgaaaaa gatttggcta tctctatttc tttggaagca
     1321 tctgaattgt tagaactttt tcaatggaaa acgccggaag aagtaaaaga aacacaatta
     1381 gaacgaatca aagaagaact ggcagatgta ttgatctatt catacatgat ggcagacaac
     1441 cttcacctgg atctagatga gatcattgaa gaaaaattga tcaaaaataa tttgaaatat
     1501 cctgtagaaa aaagcgcagg tgtacgaaaa aaatacacag agttgtagaa atgtgtcatt
     1561 cggctgttgg aaagagcaaa agaaaaacag taaaatgagt ctggagggag agaaaatgaa
     1621 aaaaaaagga aaagcaaaaa gatggctagt caatatcctc ttgttcctct tgttattagt
     1681 aggactagct ttgattttca atgaacagat caaggattat tttgtcagag agacaggaga
     1741 taaatatgct atagcgaatg taacaaaaga agatctgaaa aagaataacg ataaagatgt
     1801 cagttttgac tttgatgcgg tagaaccaat gacgacagaa ggggtcatga ggtcgcaaat
     1861 gagaggaaca gatctaccag tgattgctag tatcgcagtc ccttcagttt caatcaattt
     1921 accggttttc aaaggcttgg ataatacgtc actactttac ggagcaggaa cattatctcc
     1981 agatcaagaa atgggaaaag gaaattatgc cttagcaagc catcgtgcaa cgaaccctga
     2041 attgctgttt acaccgctag aaaatttaga gatgggtgcc aagatctatt tgacagattt
     2101 agaaaatgtg tatacataca aaacattttt taaagaaaaa gttgctccta cagataccca
     2161 attactgaat gaagtcgagg gaaaagaaat tgtcacacta attacttgtg gcgacatgga
     2221 cgccgtcacg cgactagttg tacaaggaga actggaaagt gttacatcga taaaagatgc
     2281 gacagatgat atgagatctg ctttcaatct agagacaaaa acattttagg atactgggac
     2341 ataagtcgtt acagattaaa aacagccaaa ggatatgaat cgataaggaa aaaccaattt
     2401 gctgaaaaaa agagaaacgc aaagaggtgc atctctgatt tggttgtttt ctagatagac
     2461 ttgtgttcca gtttttttat tttttatatg aaaaggcttg cataaataaa aattcgatgg
     2521 tatgatgatt ttgaataaga aatggattgt tactgtacgg caggcaaaac cagaattcag
     2581 ctaagaaaca gtaggcgaat tctaggtttt tttatttttc ttgagagggc gaagcaaaat
     2641 gaaaattgat aaaattttga ataacaatgt cgtcatttcg aaaaatggat tcggggaaga
     2701 agtcgtttgt atgggaaaag gactagcttt tcaaaaaaag attggggacg agatttcgcc
     2761 agaagcggtc caaaaagaat ttgttttaag agattctttt gctaaaaacc aattccagca
     2821 gctgatggct gatattccgg cagaggagat cgaatgggtc aaacaagtag tcgaactggc
     2881 tgaagataaa ctaaaagttg aattttcgcc taatatttat ctgacattga ccgatcatct
     2941 tcattatgcg atcacacgag caaaagaaaa tatccaactg cctaatccat tattatttga
     3001 aacaaaaaaa ttttatccga aagaatatca agcagcgaaa gaggctcttt tgttcgtgaa
     3061 acaaaagacg ggtgtggaac tttctgagga tgaagccggt tttattgcct ttcattttgt
     3121 gaatagccaa caaggaaacg aagacatgca agtgacgatg accgctacca caatggtaag
     3181 agatatactg agtatcatca gtaaattctt cggcatcgtg ttcgatgaag aatctttgaa
     3241 ttatcagcgt atcattactc atctgcagtt ttttgcccag cgttatctca aaggggaagg
     3301 atctgacgag caagatgaat ttctctacgc attagttcaa ggaaagtatc caaaagcgtt
     3361 tcattgtgtc gaacggatca atgagtatct tcgacaaacg caaaaacagg aaatggggat
     3421 cgcagagcag atttatttaa cgattcatat tcaaagagta gtaagtgaga aaaaaagcat
     3481 ccaatcataa ggattgttac tgtatggcag gcaaaacctg gattgaaaaa ataggttcac
     3541 gaatgtttgt gactgttttt ttcaatctgg gtttttattt tttaaataaa aaagaaagaa
     3601 ggaaaaaaca atggataatc aagcagttgg tcgtcgtgta tgggaagcgg taggaggaca
     3661 aaagaatgtc aaaagtttag tacattgtgc tacgcggttg cgattcagat taaaagatga
     3721 atctttagca gatacgcaaa agttaaaaga agacccggac gtgatccaag tcgtacaaag
     3781 tggcggtcag tatcaagtag tgattggcag taatgtagca gatgtttatc aatcgatcgt
     3841 ggatgaggaa ggtcttgcag tttcggagga tggaaaagaa gagtccaaaa atattttgaa
     3901 tcgtctgatc gatattatct ctagtatatt tacacctttt ctaggggcga tggcagccgc
     3961 cggggtattg aaaggattct tatcattggc aacggtgatg ggctggctga cagctgatag
     4021 cggtacttat caaatattgt ttgctgcagc agatggtgtc tttaccttct tacctgtcat
     4081 gcttgctttt acagcagcta aaaagttcaa agccaaccag tttttagcag tagcaattgc
     4141 tatggcactt gtgtatccag ctatcacagc agttgctggt gcgggagaag ctatcagttt
     4201 ctttgggatc ccagtgattc tttcaccatc tggttatact tcttctgtca taccaatcat
     4261 tctagccgta tgggttcaaa gtaaattaga gccattcgta aaaaaagtga ttccgcaatt
     4321 tctgcaaatg attttagttc cgcttgtcgt tttagtagtg atggttccat taacatttct
     4381 tgctttagga ccaatcggga cggttgccgg taatgcttta ggcggtctct tcaactcgat
     4441 ttatggattt agtccaatcg tagcaggtct aatcatggga agtttatggc aagtattcgt
     4501 gatgttcggc atgcattggg gattcgtacc gatcatgttt ttgaatatcg aacaatatgg
     4561 gtttgatgta ttgatgccaa tgcttcttcc agccatcttg gcacaaggtg gggctgcttt
     4621 agctgtagca ttgcgaacaa aagatacgaa acttcgagca ttagggattt cttctacagt
     4681 gacttcgtta tttggaatca ctgaaccaac tgtgtatggt gtgacattac ctttgaagaa
     4741 accattcatt gccgcttgta tttctggagg tattggtgga gctattatcg gattttccgg
     4801 agtaaaagct ttctccagca gtttggtcag cttattgacg attccgactt tcattaatac
     4861 ggtggatggt gtagagtcaa atgtgacagt agcagtaata gctacaggaa ttgcttttgt
     4921 tcttgcattc gtaggtacat tgattttagg atttgacgaa caaacacaag acaatcaatt
     4981 agaaaacaaa catgcgaatg caggaaaacc gattacttca gcacggcata ccttaaaaag
     5041 tcctttaacg ggtaaagtac tgccattgag tgaagtacca gaccaagttt tttcttctgg
     5101 cgtgatggga aaaggtatcg ctattgatcc agaagtagga gaactagttg cacctgcaga
     5161 cggagagatc acgactattt ttccaactgg acatgctgta gggatcacaa cgactgacgg
     5221 agcggagatt ttgattcata tcgggatgga tacagtagaa ttaaatggga atggctttga
     5281 gatcttagtc aaacaagggg atctcgtcaa agcaggagat ttattgattc gttttgacat
     5341 cgaagcaatt agagcagctg gctatagcgt aatcactcca gttgtcatta cgaatactga
     5401 tgcatttgcc gatatacttg aattagacca aaaagagatt attgcaaacg aagatgttct
     5461 ggcaattgta aaataaggag gaaatcagga tgcaatcacg tttttcagaa aaatttttat
     5521 ggggtggagc aactgctgcc aaatcaatta gaaggggctt atgatcgaga cggaaaagga
     5581 ctatccgttg cagatgctat gcctggaggt aagcagcgct ttgcgattat cggcagtgaa
     5641 gaatttgact ggacgattga tcaagaaaaa tatatttacc cgaatcatcg aggaatcgat
     5701 cattacgatc gtttcaaaga agatcttgcc ttgtttgcgg aaatgggatt caaatgttat
     5761 cgtttttcga ttgcctggac acgaattttt ccaaatggag acgaaggtac tcctaacgaa
     5821 gcgggattag aattttatga tcagctgatt gatgagtgct taaaatatga tattgaacct
     5881 gtgatcacga tttctcatta cgaaatgcct cttcatttgg caaaagaata cggtggctgg
     5941 aaaaatcgaa aactgatcga cttctatgaa cgatttgctc agacagtatt agagagatac
     6001 agcagtaaag tcaaatattg gatgactttc aatgaaatca attcggcctt tcactttcca
     6061 gctttaagtc aaggattagt aaaaagcaat ggagcaggag agtaccaaaa tattttccaa
     6121 gcatggcata atcaattcgt tgcaagtagc aaagccgtaa aaatcggcca cgaattgcgt
     6181 tcggatatcc aaatcggctg catgattatc tatgcaacaa cttacagcat cgatgccaat
     6241 ccagtcaatc aggcagcaac aatgatccaa aaccaagaat tcaatttttt ctgcacggat
     6301 gtccaagtac gtggagaata ccctgcatac acagcgcgta cccataaaaa atatggagtc
     6361 gatccggaaa aattagagca aacggaaaaa gacttccagt tgctaaaaga atatccagtg
     6421 gactacatcg gatttagtta ttatatgtct acggcaatca atgagactga tccacaagca
     6481 gcaacttcag aagggaactt gctgggagga gtaaaaaatc cgtttcttga ggcaagtgaa
     6541 tggggctggc agatcgatcc agaagggctg agaatcgcat tgaatgaatt atacaatcgt
     6601 taccagaaac cattattcat tgttgaaaac ggtcttggag ccattgatca tatggaagag
     6661 gatggcacga ttattgatga ttatcggatc gattatttac gtcgtcatat cgaagcgatg
     6721 gctaatgcag tagcagacgg tgtagatttg atgggctata cgccttgggg atgtatcgat
     6781 cttgtcagtg catcaacagg agaaatgagt aaacgttacg gctttatcta tgtggatcta
     6841 gacgatgaag gaaatgggac actgaatcga tcaaagaaaa aatcttttga ttggtataaa
     6901 gaagtaattg cctcaaatgg cgtaaacttg taattaaagc agctgaatga aaaagaacac
     6961 acttctcaca tgtgatttgt gttctttttt tattttttat gtgctattct tttagtaata
     7021 gtaaagcaaa gggtgacgtt atgaaaagga cacgtgtact atacttggaa gtcgcagata
     7081 aaattaaaga agatattttc tcaggaaaat atccagtcgg ttcgatgttg ccgacagaaa
     7141 gtgaattaga agaactgttt caagtcagca aaatcactgt ccgaaaggca atcgaactgc
     7201 tggcatcaga tgaatatgtc gcaaagaaaa gtggtcgtgg gacgacggtt ctaagcaatc
     7261 gtccttacaa tcgattatcc aaagcggcaa gctttacgca aatcttggag aactcccgat
     7321 atgaagtccg gaaagaaacg ctggatttgc aattggtcca tataaagaag gaagatcctt
     7381 tgttttcctt tttcggtgag caagctgttt gttttaagcg cttatattat ttgaacaatc
     7441 agccttatat ttattttgtt cattatcttc cacaagaaat gatgcaattc agcagagaag
     7501 aatttggcaa agaatctttg tatcgtttgc tgacgaaaaa aggatatact attgactcat
     7561 tttctgatga tttctcggca gtatttttaa cagaagaaga acaaggaatt ctgaaaacag
     7621 acgcacagat cggcatcaaa cggatacgga aatcgtctac gcaaattggg gaaatgattg
     7681 aatattcgga ggcggtctac catacggagc tgcatcctta ccatattgat tatgaaacat
     7741 aaataaaaaa agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca
     7801 aaaatagctt ctcatatttt tcgtttggtt aggcttatta ccgcagatac acggctgttt
     7861 ctggattacc atttattctg gataaggggg tgtggcacga ttttcagaag gaatctatga
     7921 accaatcact gtattgattt tagcttcgat ggtaacccca ttcggcttga cgatcgctta
     7981 tttcttaggg aaaatcattc gtaaaaatat actcaatcgt caagaaatcg atacattgaa
     8041 gaccgctttt cctatgggga tctgtcagat cacagaggga tgtttcccta tcgtattgaa
     8101 cgacttggca cgcaatgtca tagcaacggg agtcggtggg acagtaggtg gcggattaag
     8161 catgttttgg ggcgcagata gccgtattcc ggcatcggga atgtttgcag tcgctacgat
     8221 gaccagacca tgggcattca ttggtgcatt gttagctggt tcttttgtca caggaataac
     8281 cattttatta ttgaaaaaac cagtagatcc gaatgcagaa atcatccaag aagaaaaaga
     8341 ggaagaagac atatcttggg atgatttgac aattagttag gagaggcaga atggattaca
     8401 agaaaaaagt acaatagatg taccttgaga caggaatggt atgaaagaat cagagttaga
     8461 aagaatcgaa tatacagatt ttggtttagg agcgatcgaa actgtccaga gcatcggcac
     8521 cctccttgaa atgacgatga aggaaaaatg agaacctttc gttgtcgaaa gggtctggta
     8581 taccttatgt cgaaggaaag gaaacaaaaa aactttcctg tactattcca aaaaggaaag
     8641 aaagttatta tacagtaaaa aaagaaatta ttcttcatgc tagagatcag tacacgttcg
     8701 agcccaatat caaacactag ttccaggcag gcgcaacagg tgcggttatc actgaatttt
     8761 cttccagcag tgacgattca agtgatattt ttacaaatcc agctatttat cgttaacaaa
     8821 aagattttta ggagaagctg atgaacaaaa atgatcaaat tttgatcgtt tagttcgtcg
     8881 gcttttttat ttagagaaac atcatttatc agtgaaacat aacacgagaa caaaatgatt
     8941 ttttcgttaa taaaagaaac aagtatttgt tgtttaaaat aatacacgtt a
//
LOCUS       DACDSY010000119         8919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDSY010000119 DACDSY010000000
VERSION     DACDSY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.121"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="IWK44_002247"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="IWK44_002247"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9550741.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1711..3057)
                     /gene="gor"
                     /locus_tag="IWK44_002248"
     CDS             complement(1711..3057)
                     /gene="gor"
                     /locus_tag="IWK44_002248"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP9550742.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3191..4432)
                     /locus_tag="IWK44_002249"
     CDS             complement(3191..4432)
                     /locus_tag="IWK44_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9550743.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(4450..5373)
                     /locus_tag="IWK44_002250"
     CDS             complement(4450..5373)
                     /locus_tag="IWK44_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9550744.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5594..8086)
                     /locus_tag="IWK44_002251"
     CDS             complement(5594..8086)
                     /locus_tag="IWK44_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9550745.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(8093..8560)
                     /locus_tag="IWK44_002252"
     CDS             complement(8093..8560)
                     /locus_tag="IWK44_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP9550746.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8696..8769)
                     /locus_tag="IWK44_002253"
     tRNA            complement(8696..8769)
                     /locus_tag="IWK44_002253"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8733..8735),aa:Arg,seq:acg)
     gene            complement(8827..8900)
                     /locus_tag="IWK44_002254"
     tRNA            complement(8827..8900)
                     /locus_tag="IWK44_002254"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8864..8866),aa:Pro,seq:tgg)
BASE COUNT         2478 a         1884 c         1485 g         3072 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa
     1081 aatggctttc cacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca
     1141 ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa acaaatggaa
     1201 aaatagttcc tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
     1261 ctatttgttc ggaaaaagtg actaactatg acacaatttt tttcacagtc ccttttttca
     1321 cttgatttga ttcgtaccag cgaacttgac gagaactttc ttggtttggt aactatatgg
     1381 tattctttta acatcctcgt ttgcttcttc taatttattt atacaaagtc acactttttt
     1441 aaacatctgt tcttcgatac atttctattt cttttaaaca tcgataccaa tattttgaac
     1501 ttaagagtgc ataccaaatg gattcattta ctcttcgtca gttacgaaga taatcaaaat
     1561 ctccaacttc cctacttatt tcgttttgag aattatcgaa gcaatcttac ataaataact
     1621 aaaaaacaaa aaaccagaaa atcagtatac tgattttctg gttttacatg gtttgttcaa
     1681 taaaaagctg ttggaactgt taatattaaa ttatcgcatc gttacgaatt cttccgctcc
     1741 tgttggatgg atggctactg tattgtcaaa gtcttcttta gttgcaccca tcttgatagc
     1801 cacggcaaaa ccttgcagca tctcatcgac accaacacca atagcatgta agccgacgat
     1861 tctttcttct tttccgacac agatcaattt catttcgcat ttttggcgat aatcatttaa
     1921 tgcaaagtac attggggtaa agcgtgaacg ataaactttc aattcatttt cgccatattt
     1981 ttccaatgct tcttcttctg tcaaaccaat tgttgcaatt ggtggatgcg taaagacgac
     2041 tgtcggaaca agtttatagt caagataaga atctttcttt ccattaaata aacgttctga
     2101 taaacgtcta ccagctgcga ttgcaacagg agtcagatct aatttaccga tcacatcacc
     2161 aacagcaaaa atatgttcat ccgttgtatt ttggtattta tctactttga taaacccttt
     2221 tttgtccaat tccacatttg tattttccag acctaatttt tcggtattcg gtacacgtcc
     2281 accggcaaaa agtacacaat caccagttaa ggtttcacca ttttcaaata gcaccgtata
     2341 ttcgtcattg tttttagtaa tttctttagc aatatgatgt gcgtaagttt ttattccagc
     2401 ttcttggtac atttggatca aattgtccga aagcatttta tcaaacgttc taagtggtcg
     2461 ttcttttcga tatgcccaca tgacttcact acctaaacca cgaaatacac ctgaaagttc
     2521 agccgcaatg tatccagcac ctaaaacgat tgctctctta ggtaactctg ttaattcaaa
     2581 aaatccattt gaatcaattg cgtattctcc accaggaatg gtcattttac ttgcacggcc
     2641 tccagtagcg atcaagattt taggagcacg ataggtttca ccgtttactt cgatcgtttg
     2701 actatcgata aaagtagcgt aaccttttat agcctcgaca tgattgctat ctaatcctct
     2761 ttgataagca ccgtgtagaa aatcaatata tttttctcgt ctttctacta gaccggcgaa
     2821 atcaacattt tttaaatctg cttggatacc gtaacttgag gcatctcgtt caattgtttc
     2881 caaaatcgta cttgcttgcc acataacttt tttaggtaca caaccgacgt tgacacatgt
     2941 tccgcccaat tcattcgctt caatcagcaa aacttttgcg ccatgcatcc ctgctctgtt
     3001 tgctgaagca ataccgccag agccgccacc aattacgatg taatcatata tttgcatttt
     3061 gttcctcctt attgaagaga tcaatcaaaa cctttattta acgatccttt ttctacagaa
     3121 aacaagtgta acacttttct tgaattttgt atgcgaagat gcttaaaaca gggatatatc
     3181 agctttttcc ttatttacgt tttcggatca tatctccagg tttcaccggc tgtgcgactg
     3241 gcatagttaa aatcatcatt ggatttggtg ctcggtcaat ggattcgcct tcttcattat
     3301 gcataacttc aacggtttga ctgaaatgac tgaatcctgg accataaaat tcgatttcat
     3361 ctcctacaga aaacaagttt ctctgacgta tcgtagcaat ctgtgttgcc tcatcataag
     3421 ataatacttc accgacaaat ttatatactg ggattttacg acgttcaccg aataattgtt
     3481 cgttttcagt tggtgtattg tagtaaaaac ctgtagctaa ttctcgttgt gctactttcc
     3541 ataactcgtc gatccattct tgcttgcaga catagttttc aggatcttcc atataactgt
     3601 ctacagccgc tttatagaca tttgacaccg tagatacata atggatcgat ttcattcttc
     3661 cttcgatctt gaagctgtct acgccgtttt caatcaattc tggaatatgt tcgatcattg
     3721 acatatccac tgcactcatc gagaattctt cttccacttc gcctgtatca gttaaactgt
     3781 ttcgttcttg accaaaaggc atatcgaaca atccatactt ccagcgacaa gattgagaac
     3841 atccgccacg gtttgcatcc cgcatcgaca tatggttgga caatgtgcat cgaccagaat
     3901 aagagataca catggcacca tggataaatg cttcgatttc ggtatccgta tttttacgaa
     3961 tctctgccac ttcctccatt gaaacttcac gagctaaaac gacacgttcc aacccttctt
     4021 ctttccagaa ttccaatgtc tcaaaattag ttgctgaagc ttgagtggat aaatggatcg
     4081 gcagacctgg ggcttctgtc gcacaaattt cgatcaaagc tggatcagaa acaatcactg
     4141 cagaaatacc gacatcacgc aaactgcgga aaaattcccc tgctccatct tcatttcctt
     4201 catgggtgac catattagca gcaacgtata ccttcgcatc atgttctttt gcaaaagcaa
     4261 ctccttcagc catttcttca taagtaaaat tacctgcacg gcttcttaaa ccatatgcat
     4321 tcccgccaat atatactgca tctgctccat aatggatcgc ggtttttaac ttttccagtg
     4381 ttcctgctgg cgctaggatt tctggtcgtt tcaattttct ttttactgtc atttctttca
     4441 tcatactcct tatttgatag cagaaggatc aatataaaag aatcctgtat ctaattcacg
     4501 gttttcagga tgcaatttac tgatttcgct agtcattttt tccgcttttt cttgatccca
     4561 gctaccttct aaaatttgtt tttttgcttc ggcgaatagt cgagtgatgt ttacgaattt
     4621 ttcgcctgga gtaaataatc cgtccaattt ccatgtacag taatgatgct gataaagttc
     4681 attcaattca ttcgagagat tcagatcatt gctggcaaaa atatgtgtgc catgactatc
     4741 ttcataaata gaataatgtg tctcttcttt tttaggttcc gagataaaaa gacctctttc
     4801 acgatctttt tgttcgtctt gtttcgtata gttatagtaa ttctgtagca gtggacgttt
     4861 ggattgatgg atgcatgtag caccatacac aagggtttct acaggaatat cgagtttttc
     4921 ttccatcgcc ttcatctctt caaaaggaac ttcacgagca agtactgctc caactgcgcc
     4981 ttttttcgcc cagaaattga tttgacgtgc gctcgttact aatgtttcag catcataaat
     5041 atacggtata cgcaattcag gatttttttt catcgtataa ataatacctg tatcaccaac
     5101 tgtgatcaca tctacgttta tttcttttaa aaaagccaaa tattcaggta catttttcat
     5161 tttttcagga tgaatgatcc catttacagc aatattcgct tttttaccat acttgtgtgc
     5221 taattctacc aattgtcgct gttcttctcg tgtaaatgaa gcaggcagac gcaatccaaa
     5281 ttgctcttca ccgaaatata gtgtatctgc acctgcagca agcaactgtt ctgcttgttc
     5341 aatagtttct actgtggtta tgagttcgat cataattttc tcctcctaca acttcataat
     5401 agtagcatag ctctaaaaac tttccaatac ttcttctatt taaagtcgga tttttgaaaa
     5461 ttatttcaca aaaaatcaaa ataaagttcg atatattctg tccaaatata aaaagagatt
     5521 gtgacaaaaa tcttgtcaca acctcttaat caagtcttac tattataaaa attcagctac
     5581 acaggccaag taactatagg gaacctactg tttccttttt aggtccgcga acattcaatg
     5641 tgatcttacc ttttgaagca cctactgtta cgtggtcacc taaacgaatt tctccgccaa
     5701 gcaaagcctc gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg attggacgtg
     5761 caccatattc aggatcaaat ccagctttcc cgatcacatc gattgcagat ggtgtgattt
     5821 tcacttgaat atcttgttct ttcaatcgtt tgataatcgc tttgctcatg atttggacga
     5881 ttgtatggat ttcatcttta ctcaatgaat ggaagacgac agtctcatcg attcgattca
     5941 agaattcagg tcggaaagct ttctttaact cttctaagat acgtttttgc atggctttgt
     6001 gatctttagt aacatcttgg acattgaatc cgacattttt ttcttctcga atttgtgtcg
     6061 ctccgatatt tgaagtcata atcatgattg tatttctaaa gtccactttt ctacctttag
     6121 aatctgttaa atgaccatca tccaatactt gcagaagtaa attgaaaacg tctggatgtg
     6181 ctttctccac ttcatctaag agaatcactg aatatggttt ttgacgaatt ttttctgtca
     6241 actgtccacc ttcatcatac cctacgtatc ctggaggaga cccgattaat cggcttgtac
     6301 tgtatttttc cataaattct gacatatcaa cacggatcag ggcatcttca cttccaaaca
     6361 tgacttctga aagtgctttg gccaattctg tttttccgac accagttggt ccaaggaaca
     6421 tgaatgaacc aattggacga tcaggatctt tcaatccgct tcgtgcacgg cggatcgcac
     6481 gtgaaactgc tttgacagcc tcatcttgac caacgacacg ttcatgcaga agtccttcca
     6541 gttctaacaa gcgttcgctt tcttttttct ccaactgctg tagagggaca ccagtccact
     6601 ccgatacaac tgtagcaaca tcttcttctg ttacacgatc agaataacct tgctcctgtt
     6661 ttgcttcaat tgcagaaact ttttgcagct ttttacgtag tgctttttct tgtctgcgta
     6721 attgagcagc attttcaaag tcttgttttt ggatcgctgc ttccttttct tgtactagtt
     6781 catcgatttc tagtttgatc acagttgatt ttgttaagtg atcggtttga tccaaacgga
     6841 ctttcgctgc tgattcatcg atcaaatcaa ttgctttatc gggtagttga cgagaagtga
     6901 tataacgaac agaaagattg actgctgctc gtacagcttc atctgtgatc tccactccat
     6961 ggtgctcttc atatcttgta cgtaatcctt gaagaatcac ttctgcttct tcaggagttg
     7021 gctcatctac ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt tcgatatatt
     7081 tttgatattc atctagggtc gttgctccga tcgtttgtaa ttctcctcga gctaacgccg
     7141 gtttcaagat attcgaagca tcaattgctc cttctgctcc acctgcacca attaaggtat
     7201 gcaattcatc aatgaataag attacttgac catcattata gatttcgtcg atgacctttt
     7261 tcattcgatc ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat cccatatcaa
     7321 gcatcatcag gcgcttttct tgcatgtctt ctggcacttc acctaaaatg attttttgag
     7381 ctaacccttc agcaatggct gttttgccta caccaggttc acccaccagt acagggttat
     7441 tctttgtccg tcggctaaga atctggataa gccgtttcac ttctctcgaa cgaccaacaa
     7501 ctgggtccag cttgttttct tttgctaatt tcgtcaaatc acgtgctaat gaatctagcg
     7561 taggtgttcc ttgcggtgtt tgccgtttat cttgtacatt tacatttcta cgacgattca
     7621 ttccttttcc agcagaatca ccagctagcc ccattttttg tttcagaact tgacgggctt
     7681 ttgaaaggct gatacctaag ttgatcatga ttcgagtagc catgatactt tcatctttta
     7741 ataagctcat caaaatatgt tctgttccaa ctttttgaga accagttttc ttcacttcgc
     7801 tgctagctaa agcaaaaact tgtttcattc ttggtgaata tggaagaaag actcctttag
     7861 ggtaagaacg gataccacca taaccaatca gatgttcaat ttcttcataa atttccgttt
     7921 cattcaaatc catttcacgt aatacttttc cagcaattcc attttgctcg accactaagg
     7981 ctagtaaaac atgttctgaa ctaactgatt gatgtttcaa ccgttttgct tcttcttgtg
     8041 caatttctaa tactgcttgg gctctttcag taaaaagttc ttccatctaa tctcacttcc
     8101 catcttcata acttaaacgt tccagaaatg ctcggagaat ccgagcacgt gtatgatctt
     8161 catattcatt gaaattcaat gtgttttttg caattgcact caacatcaag tttccttctt
     8221 tttttgtaat tatttggtct tcataaagtt tttgaataat agcaaaagca tttttctcac
     8281 tgattgtatc atcaaataac tgatttatct gctcaagtaa ttgtttttta tctgaaatcc
     8341 ttacttttgc aattcgtata taaccaccac caccacgttt gctttctact agataaccac
     8401 gttggatggt aaaacgggta ttaatcacat agttgatttg tgaaggaaca cagttaaaga
     8461 ggtctgccat ttcaatcctt cgaatctcga tcatttcact attttccaga attttcttta
     8521 tatatgattc aattaaatcc gaggtattct gatgtgccat cttattcatt ccctttgact
     8581 aatattgacc ttaattataa cgaatttgat aatagaaaga aagaagaacg ctttttgtta
     8641 aataaatcct ttagaatcaa caaaaaaaaa tacggaatat actattccgt atcgacgcgc
     8701 ccaagaggag tcgaacccct aaccttttga tccgtagtca aacactctat ccagttgagc
     8761 tatgggcgca tattcaattt aaaaaaatta taaatcattt gaaaacaaaa agcaagataa
     8821 aaaaaatcgg gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta
     8881 ccaagctgag ctacttcccg tttaaaaaat aaaaaagac
//
LOCUS       DACDSY010000120         8890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDSY010000120 DACDSY010000000
VERSION     DACDSY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.122"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(223..1275)
                     /locus_tag="IWK44_002255"
     CDS             complement(223..1275)
                     /locus_tag="IWK44_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9550747.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1288..2226)
                     /locus_tag="IWK44_002256"
     CDS             complement(1288..2226)
                     /locus_tag="IWK44_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9550748.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(2677..3327)
                     /locus_tag="IWK44_002257"
     CDS             complement(2677..3327)
                     /locus_tag="IWK44_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550749.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(3427..3936)
                     /locus_tag="IWK44_002258"
     CDS             complement(3427..3936)
                     /locus_tag="IWK44_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP9550750.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(4137..5006)
                     /locus_tag="IWK44_002259"
     CDS             complement(4137..5006)
                     /locus_tag="IWK44_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9550751.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(5026..6147)
                     /locus_tag="IWK44_002260"
     CDS             complement(5026..6147)
                     /locus_tag="IWK44_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9550752.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(6149..6544)
                     /locus_tag="IWK44_002261"
     CDS             complement(6149..6544)
                     /locus_tag="IWK44_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9550753.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(6563..7360)
                     /locus_tag="IWK44_002262"
     CDS             complement(6563..7360)
                     /locus_tag="IWK44_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9550754.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(7362..8150)
                     /locus_tag="IWK44_002263"
     CDS             complement(7362..8150)
                     /locus_tag="IWK44_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9550755.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(8164..8640)
                     /locus_tag="IWK44_002264"
     CDS             complement(8164..8640)
                     /locus_tag="IWK44_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9550756.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
BASE COUNT         2788 a         1916 c         1284 g         2902 t
ORIGIN      
        1 tcaaattttt tctcatttta acacatattt ttattctagt tgtcttttga acaatcaata
       61 acataaaggt gagtttcttc tacattttaa aaaatcgaat ggcactgaag agttcagttg
      121 tcagcatcaa tctcctactc aaaaaggcaa aaaaacgtaa gtcagatgtt gttgacatct
      181 cacttacgca tccattgatt gaaaatttct tagtaaaatc tactagccta aaaatttgaa
      241 gacttcttct cgatcataca gtttaaatac tttctcgcat aaaaattgag taattagtgc
      301 cgcaaaaaca gggatgacta accaacaaag aacaacaaga aagccattta gccctgcatc
      361 taaagaagcc aacggaccaa caagtccgac cacgccgaaa ccagcagaag caggcgtacc
      421 tgaaatagaa aatagagcga caggaatagc tgtaatcact gcactaacta aacaaggaat
      481 aagaatcaca ggttttctaa atagattagg catcatcatc ttcatagcgc ctaaagcaat
      541 cgcaattgtc acacctgact tattaacctt ccaagagttc actactaaaa caaaagtggt
      601 tgcagctacg cccatcgcag ccgcaccagc agaaacacca tctagttgga ttgccattcc
      661 gattgctact gttgaaatag gagagataat caaaaaggca aaggaacaac caatcaagat
      721 agacattaac agcggttgta aggtagtgaa gttattaatc aatgatccaa ttgcagaagt
      781 aattgctgtt acaaaaggga ataaataaaa accaagtaaa cctgcaccta cacctacaac
      841 aatcggtgtt aacacaatcg acactgaccc aaaactattt ccaatcagta aaatcagccc
      901 gacagcaatc gatgctgtca gcatcgtatt aataatatcg cctgtaccag caccaacgta
      961 aacacccgtt tcacccaaat caggtgtgaa tttgactaca ccagaaccaa caaaagaagc
     1021 tccggctaca accatcattc tcatcggatc aaatccaaat tgtaaggcaa tcaaaccacc
     1081 aatgatcaaa ggtgttgcta gttgaaaaat aactgctaac tgtgcgatcg ttaccgcaaa
     1141 gggatacgca ctaaaatatt ttaaaatggc acctaatact gcattaggaa tcaatccaat
     1201 aatgattcct gttgctgtac cagcgaggac tttattcaga aaaattttcg gtgatatttt
     1261 ttgtgaagtt tccattcaat ctctccttta ttttacgttc aaactatctt cttttgcatg
     1321 gacaagctca gtcaagaaat cacaataagg cgtcgcaaca ccatatttct tcccttttct
     1381 agaaattgcg ccattgatat aatcgatttc tgttaatcga tggttcttaa tcaagtcttg
     1441 atacattgaa ggataatgta acccaattgt ttccgggtca aaacaagatt cacaatgtgc
     1501 aatgacttct gggacatcta gttcaatctt ctctactgct gctactttgg caaactcatt
     1561 gacaatcgtc gccaccattt tatgagcagt cgatgttttt cctagctcag ccatattaac
     1621 gtctaagatt gtgcatagtc cgttcattgt tccgttcaca catgctttgc gataaataga
     1681 atagtgaata ttgtcagaaa aatgagcatt taatcctgat tcagacaatt tgtctgctaa
     1741 tttttttgca gctgcttctt ttccatcacc taaattttgt aattctactg aaccacttcc
     1801 aaataatttg acctgaccag gaccttcaag acctgccgtc cacatggtat ttccaatata
     1861 gatattttcc attggtacaa atttctcaat aatatcttca tgcccgatac catttaatag
     1921 acataagacc tctgtatctt ttttgattaa tgattggata tcctgcagca ttttttccag
     1981 ctgcatcgct ttggtaaata gaataatcag atcaacttga tcttcttttt ctacttcgga
     2041 ttgaagaacg attggtagtt ttgcttctac ctctttacca ttaaaatttg cttgtaatcc
     2101 atgttctttg atttgttgga catgttctgc ccagccatcg attaataata cttcgttccc
     2161 actttggtga agcatcaacc caaagcgact tcccattgcg ccagcgcctg caattgctat
     2221 tttcattgat tcttcctcct ttttctttct attatactca tttgagaatc ttttcacaac
     2281 ctcgttttgt tattcttctc ttatttatca tgtttatatc caccattcaa gttattttat
     2341 cagcaaatca ataaaaactg gaatagaatc ccaatttcat cgtaagagaa actagactag
     2401 caataagaaa atagaaacat tcatagaaaa aatctcactc ctctgattat aaaagagggg
     2461 gcattaacct cgtgtatact aaaaccttcc attcattcga tggatgattt tctttttgtg
     2521 aaaaaatatt cattgtagta cgatgttgcg caacacagac ggatggtatt gcttattatt
     2581 tattaaaatc gaacaataca cttatttaaa aacttctttc ccagattttc ttccattttt
     2641 acagttataa ctagtattat catgaaaaat agccttttat tgtctatggg aataataatt
     2701 taaaattggt acttttttat taattagttc atcgcccaat aatctctcca gtttcttagc
     2761 atctcctgac tcaaccggaa ttatccaagc ccagtctaaa taatttttta aaatctcatc
     2821 atattttgca aatatttcat tgccatattc cgtccttaaa attatatttt cagtgccaga
     2881 caagtgattt atattgagtt gataaaaaag atctccatct aaaaaaatat cattagaaga
     2941 agatttcccc acagtaatga ccattccatc agctctatgt gttacataca aataattgta
     3001 tgacctcatt tcacctaatg aatcaattat ttcttgccct ttagctctaa tatattgatc
     3061 tctattacct atctcactct tatttaacaa aagttttttc atgtcaaatt taatcggctg
     3121 ttttattttt gtaaattcgc ttctactact aatccaatcc gagattaatt tttcaacatg
     3181 tcccagagta aactctgatt tattcgctaa atactttttt tgttgtatta aagacatctt
     3241 tccccgaagc tcaaaattga gcctacatcg attctctatc tcttcaaaaa gcatcttatc
     3301 ttcatcattc ttatattcat cattcataaa accaatccag ctcctcgatt aagataatat
     3361 tttaatctca tacaaaaact agctctcatc aaaacaacca ttgttttgat ttctcatgaa
     3421 atttctttat acttttctcc gattataaat tcctaacccc ttgagaaaat tacgcatata
     3481 tttatctccg caaggcttaa aatttcgttg gccttctttt ctcaaaaatg ccccgatttc
     3541 gccttttgat ggatagattt ctgcctcagc aaaaacgtct aaaatatcat cagtggtcat
     3601 agacaaggcg atttttaatt tttttagcaa cacattgttg atatatttga tttccttgct
     3661 tttttgcggc gtgtctacta tcggcttatc tttttgcgga ccacgcgcca agagaatcag
     3721 gccatttagg aatgcgtcaa aaacttggtt gttgatggtt ttttcataca cattattttc
     3781 tgcattatct ttgttcttat cctgtacttt ggtcagtacc gcctgagctt cttctttagt
     3841 cagtggtaca ccgcctagct caaaagcttt aatcaaatcc gtatctttca aatcaatcgc
     3901 ataacgcaat cgcaataacc tatcattgtt attcatttgt tcctccgaag tatttagtct
     3961 ttactttctt acgtactttt tgtcttgcta agatttttag gatcaatgat aaaaaccgca
     4021 tcacttatga taaggcgctg ggtatcatga gtgaatgata ttgctgaata caaatagtcc
     4081 gaccacatta agttgatcgg acttcataag cattttattg actaatactt ttttgcttaa
     4141 gaattgattc taacatcttt tgctaaagtt aagcatccta tagttcctgc attatctgct
     4201 aacttaggtg tgacaatgta ttcttcgata tttggcgtat cgacatagcc gttaattagt
     4261 tctaaaaaac gtctttgtac tttctttttc agatgttctt gttgcatgac accaccacca
     4321 aaaattataa tatctggtga aagcatcagg gtcgtattac aggcacattg tgcaatataa
     4381 tccgcctcga tttcccagaa cggatcttct atagaaagat cttgtccctt ggttcccgta
     4441 cgcatctcga tagcgggtcc agatgccaac ccttcgagac aatcatgatg aaaaggacaa
     4501 gtaccagaga aagtatctcc tgaatgtctc ttaactaacg tatgccccat ttctggatgg
     4561 ctaaaccctt caacaaaaat cccatcttgt atagctcctc caccaattcc agttccaaca
     4621 gtgtaataaa caacactgga taaacctttt ccatgacctg aaacatactc cccgtaacaa
     4681 gcagcattca catcggttgt ccaggcaatt ggaatttcga aatgcttatt caacgttcca
     4741 acaaaatcaa aatttttcca ggcagttttt ggagtagagg tgatatgtcc ataggtcttt
     4801 gattcacgat ggatatcaat cggtccaaat gaccctactc caatgctttc taattgttct
     4861 ttatagtgat taaagaaatc aattaccaaa ctcattgttt cattcggtgt agttgtagga
     4921 aaactcactc tttctaaaac ctttaaatca tcattgccaa tggcacaaac aaatttagtc
     4981 ccacctgctt ctattgagcc atacatttct tactccaccg tccttttatc taggaattcg
     5041 ttcttccctg tctaatagtt tttcgaaaaa ctcatgtggt tccttttggt accaataggc
     5101 aacagaagaa atatcgtctt gacgctcata aagcttgatg tcatcatttc ctagggtttg
     5161 gaaagtaaca cgtaaatttt ctttgaaact aatggggtcg ggtaaatgcc agcggtacaa
     5221 cccatgcatc ggtaaagcat catcgccaaa accatgaact ggatttggat taggttttcc
     5281 agcactaaaa taatcccggg tgtgatctct tttagattga aaaggatagc ccataaatag
     5341 cgtattaaaa ttttgagcat atgccctttc tccaaagttc tttttatgga aagcccaagc
     5401 gccgccaaaa taatcttctg ctcctgtaga tgttattgta gggaaatctt catctccatc
     5461 tatataaaac ttaaattctc cttcccccca ccagtatctt tccaatgcgc acaaagcaaa
     5521 gtaggttcct acataatacc cagtgccttt gattccatca ataagggtgt aatcttttcc
     5581 ttttgtcgtg atgggttcac ggttccattt agcatgaaaa tacatcgcat catccggtac
     5641 ttcagtcaat gcataattaa atgtataaaa aatgtgctta ataaactcag gatgttcatt
     5701 cgttaaagta atcttagctt tttttcgaaa tggcatctca aaatagcaat tcatccctga
     5761 tgttggattc actacgattg gtaaggaatt cacttcgcat cttgtcccaa acccattaca
     5821 aaaaaaatct cctaatggac tttctacaga aggagtggat tcatcatccc aataaatacg
     5881 caaaattaaa tcacgtaaga cgaaactccc agcgattgtt ttgtctgtca ctgtcatcca
     5941 aaaatgacgt aaaattccag ggccttccat ttctgctaaa gtaattgtct ctccagcatt
     6001 tatcggaaca gaaggtgacc cttttctccc aattcctaga tgactcgctg tcattgccgc
     6061 tttccctttt tcaccatttt tattttctgg tgaaatagtt ctactttcct ctcccttgtg
     6121 gagccatata ttatttaaac aattcatttt attcgtcctc tatcctttct tcactttttt
     6181 gattattcat atagatgatt tgttgttgtg cctccgcaat aattgctcca atatctaaat
     6241 tatcatttcc taactgcaaa gcgattgaca ttgccaaggg aagattcatt cctgcaatta
     6301 cgtgcactct gtcagaaata taggtaaaaa acttttgatt tacacttcct ccagccatgt
     6361 cagttaaaac aattatttcg tctgtagtat ccactaactc tttaatttgt tcatctatat
     6421 catcaatttc ttcgacatat gcacataagc tactaatttg attatgcata cctgttaaaa
     6481 actctagtgt ctctttaaaa ccctcagcta gtattgaatg gctagcaatg atcaatcttc
     6541 tcataattca aatccttttc ctttacgcta atattccaaa tgcagccata accatcgcaa
     6601 aaacaataac gagtagaatc aaaatggtca tactgatttt tttcttactg agtagataat
     6661 atgctgcagc agtaataatt actggaatta atgttggtaa aattttgtcc aacattggtt
     6721 ggattttcat actaacttca ccaaaagtga atttaagtgg cgttgaaaca ctgataactg
     6781 aagcaattaa tgatccaact actgttagcc ccaatactga agcagcttca gttaaataat
     6841 taatcttttc accgtattca gtaacaatct tcattccctg cttataacca aattcaaagc
     6901 ctctcattcg gaaaaagaag attaccatga ttaaaatgaa ccataaaaag attcctacag
     6961 gattattttc taaagccata taagccgcaa ttgatcccat gatggttggg atcatcgtca
     7021 tcaaaagaga gtccccaata ccagcaattg gacccatcgt accaacttta aagtcattaa
     7081 tcgcatcttt tgcttctaat ccttgctttt cctccattgc aacacaagca cctaaaataa
     7141 tggctgccag ccaaggctgt gtattgtaat actgaaagtg attatttaaa gcttcgatgt
     7201 attcatcatc atctttgtat aattttctta aaataggtga caatgcgtaa gtcacagatg
     7261 caccttgctg tgtttgataa ttaaaggtac aaactgacat caaccaacga tagttcgctt
     7321 tcctaagatc ctttttcgtt acttgatatt tttcgctcat tttattcatc ctccccaata
     7381 aacccaacat cagaagctcc cgcagaagca gtgatttgtg catctttttc agctctatga
     7441 tacacattaa atgctaaagc gaatcctatg atcgccgctc caataatagg taccttcaaa
     7501 aaagcggcta agaaaaatcc aacaagtaaa taactaatgt atcgacctgt aggcataaag
     7561 cgtaacaaca tcgcaatacc aacagcaggc aacatttttc cggctaatgt tagcccatcc
     7621 ataaaccaat taggtaaaac atccaaaacg taattaacag cagactgtcc taaagtaacg
     7681 gcaataaata ctggaattgc tgcagataaa ccaaagaaaa ttggccccaa ccacaaaaca
     7741 ctattcattt tcttaaattc tcgtttattc gcatattcct gtgatttgcg aacaacaaaa
     7801 ccatttaaga ttttgacaat tacatctagt tgcaccccta acatacctac aggtagtcct
     7861 attgctaatc cggcttctac tccaagctgg ctaccaaatg ccgttgcaat aattgcgcac
     7921 gttccatagt ccggtacaga tgagccacca aaaccggcaa ctcctaatga catcaattga
     7981 actgtaccgc caactatcaa acctgttttt atatcaccca tcaaaattcc tgtgaataat
     8041 ccccaaaaaa ctgcgtaatt tacaaagatc atcggaatcc cataataatc aacatgttta
     8101 ataaatgcta atatagtcag cataataatt tgaaaaatag taaaatgcat tatccttact
     8161 cctttatcct aattttgatt gaatttcctg tgccttatcc gccggaacaa attgtgtaac
     8221 aatttctaca ccagaatcca acaatttttt atacgtgctg atattttcat ctgatgcata
     8281 ggctctagaa gatagctgta atgcattatc tttaacaaca gtaccgccaa caattagtcg
     8341 gtcaattcta ataccgttat ctactaaatg aaggattgtt tccggctctt tcacgatgac
     8401 aaatactttt tcctgatcgt atttcccctt tttaaaattc gcaatcgctg tttcttcagt
     8461 gataattgat acagccattc cagcaggtct agctaaacgc atacttgatt tttggatgtc
     8521 gttatttgcg acagaatcat cgatcaccat cactctgttt gctttagaca ttggtgccca
     8581 ttgcgtgaca ataatcccat gcaataaacg gttatcaact ctcgacataa caatactcat
     8641 aaatacttcc tcctttatct ttcattttct tacaagcaat taccatgcca aaacaaaaag
     8701 aacttgataa atcaagctct ttttacataa tcataaacgt atttaacctc atgaatcggg
     8761 atgtcgacac tataataatg ttcaacgaca ctgaaagatt gcttaaaatg tgtctgaaat
     8821 ttttgaaatt cctcactcgt ttcattgaaa taaatttcat cttccaaagt attttgtttt
     8881 actaatcgtt
//
LOCUS       DACDSY010000121         8851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDSY010000121 DACDSY010000000
VERSION     DACDSY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.123"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            63..1277
                     /locus_tag="IWK44_002265"
                     /pseudo
     CDS             63..1277
                     /locus_tag="IWK44_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1456..1791)
                     /locus_tag="IWK44_002266"
     CDS             complement(1456..1791)
                     /locus_tag="IWK44_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9550757.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1775..2047)
                     /locus_tag="IWK44_002267"
     CDS             complement(1775..2047)
                     /locus_tag="IWK44_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP9550758.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2498..3301)
                     /locus_tag="IWK44_002268"
     CDS             complement(2498..3301)
                     /locus_tag="IWK44_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9550759.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3279..4403)
                     /locus_tag="IWK44_002269"
     CDS             complement(3279..4403)
                     /locus_tag="IWK44_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9550760.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4419..5234)
                     /locus_tag="IWK44_002270"
     CDS             complement(4419..5234)
                     /locus_tag="IWK44_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9550761.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5224..6105)
                     /locus_tag="IWK44_002271"
     CDS             complement(5224..6105)
                     /locus_tag="IWK44_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9550762.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(6181..7470)
                     /locus_tag="IWK44_002272"
     CDS             complement(6181..7470)
                     /locus_tag="IWK44_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9550763.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7698..8342)
                     /locus_tag="IWK44_002273"
                     /pseudo
     CDS             complement(7698..8342)
                     /locus_tag="IWK44_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2742 a         1883 c         1410 g         2816 t
ORIGIN      
        1 caaaatcaat tctaccttag atgttcttat atcactctat cagtcctatt tgacaaagag
       61 ccaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa
      121 gttgtggttc tactgtggta gatggaaacg ggaaagcaat tatagtgaaa aatgggaaga
      181 aggaaacgat tgtccgtttt gaacaataca atcatatgcc tttggttacg cgcctaaaaa
      241 agcaacgcta tacctgtaaa aactgtcgta cccattggac ggctcaaagt tattttgtcc
      301 gactcagcca ttcaatcgca gatcatgttc gatataaaat tgcttcttta ctgactgaaa
      361 aagtatcttt gtcttttatt gcgaaaagtt gtcaggtatc tttgactacc gttactcgta
      421 cattgaaaga gtttaaaagc tatttaccaa agccttctaa gaagattctc cccagagtat
      481 tgatggttga tgaatttcgt tcgcacgctt ccatagaaga taaaatgagc tttatttgcg
      541 cagatggtga aacaggaaac ttaattgctg tcttgcctac gcgtaaatta cctcgattaa
      601 caagctattt cttaggctgc accaatccag aagaagtaga attcttagtc acagacatga
      661 acgccgctta cttccagctt accaaacgtg ttctgccaaa tgcgaaagta gtgattgatc
      721 ggtttcatat tgtcaaacac atgaatcagg cactcaatga gttgcgtatc cgtgaaatga
      781 atgagcttcg taaagcagga cagaaaagtc aggcagaaaa actgaaaaag aactggcgct
      841 ttctgctaaa aaatcgtgca agcatcaacc attatgagta caaaacatgg aaaagtttcc
      901 gagcaccaaa atacccattt ctcactgaag caatgatgat tgatcgattg cttgagtttt
      961 ctgcgcctct aaaggaggcg tatccctttt ttcatgaatt agtcgaagcc tttcgagaca
     1021 aagaccctga cttatttttc tccttattgg cagaacttcc cgaaacgtta gatgacggct
     1081 ttcgggagaa gcttcaaaac cttctgacct atgaagaagg tatcaccagc gcaatgatct
     1141 atccttattc caatggaaaa attgaagcga agaataccca cataaagaca atgaaacgag
     1201 tatcctacgg atttaaatca ttcgagaaca tgagaattag aatctttttg atcaatcaat
     1261 taatcaatgt aagataacaa aaaatctgaa ccagaatgtg gttcattctg acccggattt
     1321 gattttgcac aactcatcag tccttattga caaagagcca aaaatctgag ccagaatgag
     1381 gttcattctg acccagattt gattttgtac aacccatcag tccttattga caaagagccc
     1441 ttctttttag tattattata attcttccat gcgttcttcc agtcgctgta aatcgcttgg
     1501 acttaattcc ccaatttttt tccatttgac ctttttgttg tttttgggta attcgtttag
     1561 agttcctacg tcaatccaag attctttttt tagtcctgcc tgtttccaat cccttatttc
     1621 aacgtatttc agctttattt tatcggattt agtatgatat tttgaagtaa ttgacagaaa
     1681 cgaaattgtg tccagtccat tttttagaat atagacaggc cggcgtttac cgccctctgc
     1741 agctgtgaaa gcaacgtaga cggttgctat atcattagta ttcatcttca ttgaaccatt
     1801 cttcaatttt ttcgtcagtg tctagttttt ggacaggcaa tttttccgct aatctttgaa
     1861 tattatggat tcgtttactt ttttccgaca attcaataga aaatgggatt tttccttgct
     1921 caacaatttg cttataaaaa acggtgacgg ctgttttggg gctcatgcct agttcatcaa
     1981 taatgctttc tgcttcattt ttcaattttt cgtccataga aatttgtaac ttagccattt
     2041 tctccacctc tttctgacaa acacattata tcacggaaag agttcaaatg gaattaatac
     2101 aactactttt gaactccttg aaatgcagtg tatttatcta aatataagga aaggatatag
     2161 tgctaaatgg ccttgataga tagaaaagtt tcaaaatccg ccacagcgat aattgaagcg
     2221 aacactcaac gacacacaga acaggaaaag actcctaaaa gaaccaaatt aactaggacg
     2281 tgtctgaaaa ctccacaaag atgtggtatg ctctggtaaa aaacgaatgg cacaaccacg
     2341 aagaccgata tggttcttgg aaaactttct ccaccctttt tgtaattgga gggatagtgg
     2401 tttattgata gcgattttcc aagttcttca agtggaacca gacattgagc accacggcta
     2461 aaaagtgaat gaagtcttta tttttaatca ataattttta atgattacca gtaattggtt
     2521 ttttcatgcg ccctatttct atttgatgtg gagttaaata atgctgataa gtaaaaattt
     2581 ttccgtcaat taaactgcca ctagtgaatc ctgttctatt ggtaaaaaga gaatagtctg
     2641 ggtattcatc tacaccaaat gataaagatt cagccataaa ctgtttttta ccagcaaatt
     2701 cagcactgct accttgatgg atatgtccta caaaaatacc taaaatatca ctttcggcaa
     2761 tcaattcagc aaaaccttcc ggaacttcag ttacaataga tggatcttcc cacgacaatg
     2821 gatgatgaac caataaaatc gttccctttt cagcaggtgt acccaaccac tcttgtaatt
     2881 gaatcatctg tcctatcgat atttttcctt cgtgctgggt atcttgagca gaatccaagg
     2941 aaataattcg taacccattt tgattcgtac aatggatata ctctcccgtt tcattgcttt
     3001 caacactcat tccttcaaag aaaacactgc gacgatcatg attcccacga caaaaatagt
     3061 aaggtgtggt tgggaaatat tgatccaaca ttttccgtaa gacttgataa tcagaaatcc
     3121 ccccttcatg tactaaatca cctgttaata gaataaaatc gtacactgtt aacgagagtt
     3181 cttgtaacaa tttttccaat gccaactgtg gatttttaat tgttggaaat agttcagcag
     3241 taaacaagtt agtttgatag ttttctctaa aatggatatc agagagatgc aaaaagcgca
     3301 tggctattcc ctctttcttc aataaaataa tcaggatatc ctaaattttc ttgcgtctga
     3361 taatcaaaaa aatgcattct ttcaggttct atgcggagat tgactgcatc accaatctgc
     3421 caagtaggtt tttcattaag gacaatcatt tctataccat cgactttaac ggtgtacgca
     3481 taactttgac ctaatagctc acgatacttc acttgaccgt gtaaatcgct tgtttctttt
     3541 gaaaaggtta cgtgttctgg tcttatgcct aaatacaatg agccactaac ctgaagaagt
     3601 tgtttttgcc aagctggatt caatgttaaa aattgactgg ctacctttaa tgttccttga
     3661 tcataagtaa ctggcataat attcatgcct ggcgaaccaa taaaatttgc agtaaaagta
     3721 tttttaggtt tcgtataaac atttttcggc gtatcaatca tctgaattct accttcattc
     3781 aataaaacaa tccggtcagc taacgtcatc gcttcaattt gatcatgagt gacgtagaca
     3841 aaagtttgtt tataacgttg atgaattttt accaattctt ttcgtgcatc caatcgtaaa
     3901 cgcacatcca aatttgataa cggttcatct aataaaaaat aattactccg tttaaccacc
     3961 gcacgagcca atgctacacg ctgccgttgc cctcctgaca aatctttagg aagtcgatct
     4021 tggtatggcg ttaaacctaa catcttcaaa gcttcttcca aacggtgctg aatgtcagct
     4081 gtcttaattt tattcgccct taatgcaaaa ataatattct cctttacagt catgtgagga
     4141 tataaagcat aattttgaaa aaccattgcg atctctcgtt ctccacaagg tagcttattt
     4201 gctagttgtg tacctaaaaa aagctctcca ctcgtaattt tttccaaacc agcaatcatt
     4261 ctcagcaaag tagattttcc tgaaccagat ggtcccagta acacaattcg ttctccagcg
     4321 gccacagcta aatcaatttc ttttaaaatg gtatgttctc catatttttt tccgatattt
     4381 cttaattcaa gcgtttctcc catgatttgt cctccaattt atttaatacc tgcagaaaca
     4441 aaagtcccga taatatattt ttgaaaaatt gcatacaaga ctaacggtac aaacattgta
     4501 attaaactaa tcgccattgc tacggtaaaa tttgtcccac cttcagaact aatataggtc
     4561 tgcatcgcca acggcaaagt ataattatct actgtccgta aaattaatac cggccaaaca
     4621 tactcattcc aagaggtaat aaaaaaccaa ataccggtag aagtaatcgc atgtctagta
     4681 agaggaaaaa caattttact catgacggtt gtttgtttga tgttatccaa tgccgccaca
     4741 tctagaaggc tcactggaat ttcccgcatt gtttgcgtca ataaaaaaat gcccatcgca
     4801 tcagcaaatt gtggtattat cacaccccaa gtagtatcaa ttaaattaag ttttgcaatc
     4861 aacaaataat taggaatcat cgcaacagta aaaggaatga acatcgtacc aatgatgata
     4921 aagtaccaga ttttcttccc tttgattcgg caataagtaa aactatatgc cgcaaaaaaa
     4981 gctacgccta acttaacaac cgttataatt gtagcaataa aaaaagtatt tctcgtaatt
     5041 ttgataattg gaagattttc gaacaaatag caaaaatttt ccaaggtaaa cggctgtggt
     5101 atcaaactag taatcgtctg ataggcatct ttcaatggct taaaagaatt ggccataata
     5161 aacaataatg gatagataat aatgattatg atgaacataa ataagagatg tggcaaccag
     5221 cgtttaattt tcatagtaaa cccccttttc cacaaattta aatattaccc ctaagaaaac
     5281 agcaaatatt aataaagtta aaactgaaat tgctgaagat tctccgactt tatacatttc
     5341 aaaagccttt tgataagcat aatagatcac gtttgcacta ccattattcg gtccaccacg
     5401 agtgatgatc ttaatcggag taaacacata ctgcagacct tgaacaatgg tcatcaccaa
     5461 taaatatagt cctgtcgaac tactcatagg caacacaatt gtaaaaaaaa tgcgccagat
     5521 gggtacacga tccaacttag ctgcttcgat catctcttga tcaattccca caattgctgc
     5581 aaaaatgaca atgaaattat aaccaaaaac tttccaagca gtaatacctg caatcacagc
     5641 aattacccaa ccttgtaaat tcatccacat aggcagtctt aaaccaaaat ttcccaacaa
     5701 gacagcaatt ggtcccgata ccggatttaa tatccatgta aaaaccatcc ccgccactac
     5761 caaagaaatc acagctggaa taaaaaataa tggtttatac aaattctgcc ctttttctat
     5821 aactattcct aataaaaagg atgtaatata tgggaacaca cagctcagca agacaaatag
     5881 aacggtatag gacaaagtat tcttcaacac tttatgaaag actggatcag tcagcacctt
     5941 tacatagttg gcaaagccaa caaattcttt agtgggggcc accatattcc agtcaaaaaa
     6001 actcaaatag atcgtataaa tcaatggata aaaaacaaac acgcccaaca aaataattgc
     6061 tggcgccaga tacaaataac ctacatagcg cttcatttcc gacagcctcc tcatccttat
     6121 gtcaaaagca gacgaccttg tttttccttt cttacaggcg ctgtcgtctg cttgctttgc
     6181 ttatttgagt aactcattaa tttccttttg agtagctttc atcgtttcat tgacggaatt
     6241 acctgataaa attttatccc gcatatccaa caatttttgt tcagcttcta atccagcatc
     6301 tccaggaaat gaactccaac tgaccatctt atccatctga tcaattgctg gtatcatcat
     6361 ctgattttca gctaagaaag cttttaatcc attttgggcg tctgcaacat ctttacgtgg
     6421 tggtaaataa ccagtacctt ctgtccattt agctaaagat tcaacactat aaagatattt
     6481 taagaaatcc caagttgctt cctttttctt atccgtttgt gatgtaacag ctaacattgc
     6541 gccaccagca ggtagtttaa cttttttccc ttcccaactt ggtgactcaa ctgctttagc
     6601 agtaaaagta ctattttttt gaatgttgtc cctttgagca atagtggtat agaccatcgc
     6661 gacgttacca tcaataaagc tttgcacacc ttgttcccac gtagtgtgta atgccgtctg
     6721 gtcttttacc atcctttgat ataattcata agcagcgata ccatctttag aagcaaaggt
     6781 agctttgcca tcttttatta cacttgcccc attactttct aaaattgctt gttgtgccca
     6841 attatcagca ggttcttgaa tatacacgcc atactttcca gttttttctt ttattatctt
     6901 agaaaaagtt tgaacttctt cccaagtttt tggaccattt tcatccaagc ccgctgcttt
     6961 taatatatct tgatttaaat ataaaaccgg attactaatg gagtaaggaa tacccacttg
     7021 tttaccatct ttttcagaca gtgccatgat attgtctaaa aaatgatctt taaaataatc
     7081 agcattatcc ggatccactt cttctactaa agattgagga ttagtatatt tgaaattttc
     7141 tgcaaaataa tctttaaaag cccatcccac ttgtactacg tccggtattt ctttggaggc
     7201 aacagaagct tgtaaatttt gcatcaagcc tttatacata tcaggattat attttgctac
     7261 aaccttcact ttgtcttgag attgattgta tttatctact aattcattca ctgtttttcc
     7321 acctagggta tccgcattaa catgccaata ctctatcgtt acacgaccct ccgatttttt
     7381 tgttgtcttg gtattcccac taccacaagc tgccaaagag atgacactta aaacagacac
     7441 aacccctata acaagatttt tctttaacat aacattaact tcctcctaat ttctttatgg
     7501 ccatttttct aatcaagaaa tattatgaac taccaatgta aatttctagt taattttctg
     7561 taaataattc gaaatttgtg catttcgggg tacagggtca ctggacatat ccatcaagct
     7621 aagaataaag cccccatatg gtcatgtatc ccatctccaa atctcttaac tcctctgttt
     7681 tgattataaa atagtttatt ctgtgctcct ttcttccata aataggccca tgcacaacgt
     7741 gaggttatat tataaacacc acagcgcgca aggtactcaa tcgtttctag ttcattgact
     7801 gtccatacag aaaatgtcaa tcctagctgc tgccccactt cacgggccaa cttagttaac
     7861 catccttcat gaatattgac gatttgaacg ctttgatagg ctaatttctg taaggtatcg
     7921 atcaaatctg cttcggtaat atccccaagt tttttccctt cgaactgatt ttcaatatta
     7981 taaaatatat gctttcccca aatcgcagcc caagataatt gtacatagcc actaagaaca
     8041 atttgtggcc agatttgata ggtttgtgcc agcgaaaaaa cggccatttc caatccctct
     8101 tcttttaaat cacagttaat ggtcatggtt gccttctcat tggccactgc ttgaaacacc
     8161 tgctcaaggg ttaccgaatc tgggacaact tcttacctgt catgggctag tattagcgta
     8221 ccatctgttt gctgacgcac atctacttca aaacaaagct ggcgatccaa acaaaagcga
     8281 agaaatgccc agctattctc ctccgtattg tcactaccag agtgtgcagt gatcatggtc
     8341 attttgaccc ctcctttaat tccgtgattt tctcccacac gtccactata ccgatgaagc
     8401 gttaatttct tgttaatttc ttgtaaattc cctgcgtttt ttcagtaaac tagttgtaaa
     8461 tcttgcatgc tccttcctac gaactcatga atcgtcgatt ttgcccttct agctagtttc
     8521 accaattaag tagttctaca ttccccgctt ttaacctact acaccttttt ttgttttcag
     8581 ctattccttc ttcttgctga aagtccttga ggttctttct tacaggatac catcaaggtg
     8641 ttcaatggct tattcccatt gaatgaccga gacttactat cagatttctg ctcgttgaac
     8701 caagtctttg atacaggatt tcttccgtca tcttctcaca aataccacct tgcgtttctc
     8761 tagcgattgg tcgatgtgca ccctcccagt ggactgtcac tacccctttt tgctatgcct
     8821 agcccacaaa aaaagctgtc ctcacagaca g
//
LOCUS       DACDSY010000122         8740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDSY010000122 DACDSY010000000
VERSION     DACDSY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.124"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..426
                     /locus_tag="IWK44_002274"
     CDS             <1..426
                     /locus_tag="IWK44_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP9550764.1"
                     /translation="AVFILFGVLFYTQIATSEKGFNFDWQTTLFVSVIYFALLLGIVI
                     AYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFMKVADLDDAPGL
                     MLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            435..662
                     /locus_tag="IWK44_002275"
     CDS             435..662
                     /locus_tag="IWK44_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9550765.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1298..1669
                     /locus_tag="IWK44_002276"
     CDS             1298..1669
                     /locus_tag="IWK44_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550766.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1925..2167)
                     /locus_tag="IWK44_002277"
     CDS             complement(1925..2167)
                     /locus_tag="IWK44_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9550767.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2199..3101)
                     /locus_tag="IWK44_002278"
     CDS             complement(2199..3101)
                     /locus_tag="IWK44_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9550768.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3114..3302)
                     /locus_tag="IWK44_002279"
     CDS             complement(3114..3302)
                     /locus_tag="IWK44_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9550769.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3316..3879)
                     /gene="amaP"
                     /locus_tag="IWK44_002280"
     CDS             complement(3316..3879)
                     /gene="amaP"
                     /locus_tag="IWK44_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9550770.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3917..4810)
                     /locus_tag="IWK44_002281"
     CDS             complement(3917..4810)
                     /locus_tag="IWK44_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9550771.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4888..5826)
                     /locus_tag="IWK44_002282"
     CDS             complement(4888..5826)
                     /locus_tag="IWK44_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9550772.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5860..6210)
                     /locus_tag="IWK44_002283"
     CDS             complement(5860..6210)
                     /locus_tag="IWK44_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9550773.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6243..7145)
                     /locus_tag="IWK44_002284"
     CDS             complement(6243..7145)
                     /locus_tag="IWK44_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9550774.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7138..7995)
                     /locus_tag="IWK44_002285"
     CDS             complement(7138..7995)
                     /locus_tag="IWK44_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9550775.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8329..>8740
                     /locus_tag="IWK44_002286"
     CDS             8329..>8740
                     /locus_tag="IWK44_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HAP9550776.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQN"
BASE COUNT         2442 a         1677 c         1489 g         3132 t
ORIGIN      
        1 gccgtattta ttttgttcgg cgtacttttt tatactcaga tcgctacaag cgaaaaagga
       61 ttcaattttg attggcagac cacattattt gtttctgtca tttactttgc ccttctccta
      121 gggatcgtta ttgcttattt cttattgaaa ctattgaaaa aaatcgaaga taaccaagct
      181 ttttccactc aaagcttaaa catactaaaa aaaatcgaac gaaccatttt gtttattggt
      241 ttcgcctttt ttggcttact tcctaaattt atgaaagtag cggatttaga cgatgctcca
      301 ggtttgatgt tggtaggaat cgctgtcgta ttcattcctt ttgcagtata cacattaaca
      361 agcgtgctgg aaaaactgct tgaacatgcg attttactta agacagatca tgatttgaca
      421 gtgtgaggtg cgacatggca attatcgtta atttagatgt agtattagct caaaaaaaaa
      481 tgagtgtgac tgaattgagt gagaaagtgg gcattacgat ggcaaacatc tctattctaa
      541 aaaatggaaa agcaaaagcc atccgttttt ccactctcga aaaaatctgc caagttttgg
      601 agtgtcaacc aggtgatttg ttgacctata aaaatgactc gaatactata ccagaagaat
      661 aaaataaagt agaacaaaca gtaaataaaa aataatctga atgatagatg tgtgagactt
      721 ttagaagtta tttacgtcta ttattcagat tttttatttc gattgaatac cgttttatct
      781 ataaataaga tatgtactga ctcatacaaa gggggccttt tcttctccat gtactatcac
      841 ttttcctgat acaactggtt tcctgttacg ttgtagttgg atctgctttt agtataatac
      901 tttattcatt ccaccagact gatagcagtt tgagcagtct aaataatcat gtagtattac
      961 aggccattct gtttcatacc ttgaagtgct taaaacttca gctaatggcc cattgtgtta
     1021 actcttactc cattcttttt tagtaagctc ccatatatac tgtaaagcct tctatatatt
     1081 atgtcatgtt gctttttaac atttactatg tagttttgat aaaatgattt gaataacagt
     1141 gatggacaat tcttttttat ttttaacatt attttgataa taatgattat ttatttaaaa
     1201 atacattaga taacatttgt tacgacacga aaaagcttca caaacaaata tcgttatttt
     1261 aaaatgaggg cgaactcaca caattaggag gaatagtatg ccatttattg tttacttttt
     1321 tctttcatta ctcgtgatct atattccatt accaacattt ttcctgctcg tcaatcgtct
     1381 agcagagcaa catgatacga ttgctgctct tacgaaactt tgtttacttc tatttgtttg
     1441 tctagattac tttacgagct attattggct aaaacagaca agagtaaaaa agagatatta
     1501 tttcttcctt gtctttttca atctctttga gtttctgatc cattgctttt tacttcatca
     1561 gtatcatacg atccatttgt ggatcatctg ggttttcaaa gtggtcgtat tattttgtat
     1621 gttcaccttt cctatctcaa gaaaatttag ggaggcattg ttttattaaa gaagcttctc
     1681 tgtatcaata aagacacatt taaggaaaaa cttttaaaaa tctaattggg aatttgcccg
     1741 cataaataag aggctgtgac aaaagtcttg tcacagcctc ttattccgta taaacagtgt
     1801 tcaaaagctt gcatcttcga taatatgccc aaccaaatga aaaatatgag aagctatttt
     1861 tgtttggctg ttcttattac ttgatgaaga acagcttttt cacgacctct ttttaaaagt
     1921 ctttttacag ttttcgagca aagaatgata cgattagtac taaaatgatg gcaccaatca
     1981 atgatggcac gattgccatt cctgcaatac ttggtcccca agaacctaat aatgcttgac
     2041 caagtgctga tccaactaaa cctgctacga catttgcaat aatccccatc gaagaacctt
     2101 tattcgtgat tgctcctgca attgctccga taattcctcc gacgataagt gaccaaataa
     2161 atcccatgtt tcgttcctcc taatgtttta cttccttatt actgaacccg tggttctgta
     2221 ttttctttta ctttttgtgc gccgcttcca gctgctgatt ttactttatc tgtttgattc
     2281 gaagcaaatt caccggtttt tttcgctgcg tcagttactt tgtcttgcac cgtttcttga
     2341 tctttttcaa attcttcttt tgttttgatg tccactacgt tagcgttgac ttcgatcact
     2401 tctaaatgtg tcatattttt tacttctttg ccgattactt cttgcatttc ttcaaagatt
     2461 ttttcgatat ctttgccata ttccacaatg acatccatat caactgctac ttgtttttta
     2521 ccgacttctg tttcgatccc agccgttaca ttatctgtat tcacaagttt tcctgccaca
     2581 ttagagaaga aaccaccgtt tgcagataat aaaccgtcta cttgttcaat tgcgattccg
     2641 atgatttttt gcacaacttt atcttcataa ttcagctcac cgcgcattga atcttcctgt
     2701 tcagaatcat gctttccttg gttatttgta tgatcagaac caagttttcc ttgtccgttt
     2761 gtgtggtcag aactaagttt ttcttgtgct ttcgcatggt cagaaggatg tttttgctct
     2821 tccacaggtt tagaaggatc gattgcatca tgttgttctg gaactttaga ttctggagtt
     2881 cctggatgat gaggtgcatg cttttctgca tcatgtttct tctcttctac tccttgttga
     2941 acgttaggga ctactggatc tggatgtgca gatactggtg ctgttttgtt cttttcatcg
     3001 ttcacaccat ttgattggtt tggtgtcgtt gggtctggat gtgcagacac tggtttttgg
     3061 ttgttcattt tattgttatt atttttgtta ttgttattca tggatgtttc ctcctaagat
     3121 ttaaaatagt gatctaagat tttcgtttgt ttcagataaa aaccgatagc gacgccgatc
     3181 gctgccatga tcaagatcaa taaggtcttg aaaaagccca cggtgatcca taaaacggct
     3241 aatatcaatc caagtacccc aaagatgata gggagcttgt atgtcgaaaa tagttctttc
     3301 atttttggtc cccccctatt ctactcgttt gcggtcattg cttcgtttag ctggttcttc
     3361 aaagcctgtg tatttcaccg atactttcac tggctctttt gttcccaata tgtgttgtac
     3421 ttcattttcg atttctttca tcaatgttcc tgttttacca atgagtgctg aagttcgttt
     3481 caactcccct tttacattga cggcgatctt gtttttcgtt gctttgacat ttacttttgg
     3541 tgaatggatg aattcatctt catgcaagtg agaacgaacc attccttcaa ttgctttttt
     3601 atccaacgtc aactccccat caccatgttt cagtagaaag tttccgattg tttttggata
     3661 aaacaggaaa acaagtaacg tgataattgt caaaatagca aatgcaacgg caagccaaaa
     3721 tacatattgt tgcacgtatg aattagtgat ggaaaagaat cgaaacttct ctagctgaaa
     3781 aggcaatgga taggttgcac ttaatagtat cgcaaacaaa agcacagata gcaaaagtaa
     3841 tgttccgatc actccgatcg tttttacacc tttattcatt ttcgcacctc ctcatagggg
     3901 tgaaacgatc aacttcttat aagttgatcg cttctccacc agttactccg taaatttggg
     3961 ctgtaacata acttgcttta ttcgatgcta agaaaacata aactggtgct aattctcctg
     4021 gctgacctgc acgttctagt aaactttgct gaccaaattc agggatcgaa tcagaaggtt
     4081 gtccttcgtc tagttgtaat ggtgtccaga ttggaccagg tgctacacca tttacacgga
     4141 ttcctttagg tgcaagctgt tttgctaaac cgatcgtaaa gttagcgatt gaagcttttg
     4201 ttgctgcgta atctaataaa tgttcgcttg gattgaatgc ttgaacagaa gttgttgtta
     4261 cgattgctcc tcctgctggg atatgcggaa cagcttcttt cactaaagca aacatagcga
     4321 tgatgtttat cataaatgtg tctttgactt gctcaagtgg taactcagca atagactgac
     4381 aagaaatttg ctgtcctgcg tttagtacca tcgtatctag cccgccaagt tcactcacgg
     4441 ctttatctat gatttcttta ggtgcttgtt cgtcacgaag atcaaaaggt agtaagacaa
     4501 tcttacgtcc tgcaactttc acgtattttg ctacttcctc tgcatctttt tcttctcctg
     4561 ggaagaattg aagagcgata tctgcccctt ctctagcaaa tgcaatcgct gctgcacgac
     4621 cgatccctga atctccacca gtgattagta cacggcggtt ttctagttga ttattgcctt
     4681 cataggattc ttctccacaa tcaggaacag gaatcatatt ctgctgtaaa gctggtgtat
     4741 cctgatcttg tttaggaaat ttctccgtat ggtacaagtt tcgcgggtcg attaattttg
     4801 gttctgtcat tgctgtattc ctcctagttt ttaaattgta ttgccaaaac aaatttcttt
     4861 gtttgatacc tgttttaaat agattcttta atttgtataa tctttccggt ataaatggta
     4921 acctaaaact actgctgcta tcaaagagcc aaagactacg agtataaagc ctagagcaga
     4981 acttttaccc ggtaatcctc ctgtattcat tccccaaatc cctgcgaaca acgccggaac
     5041 ggaaatgatt aatgcagctg aatctagata tttcatgata tgatttagat tattgtccat
     5101 catcccgttg aatagatcac caatactctc taataaatct cgatagatct cgatcatttt
     5161 ttctgtttgt cgctgtctca aatggacatc atagagtagc tgttcatcgt ctaaacgttt
     5221 gatgaaagtt tcagaattcc ataattgatc cattgtttcc cgttgatctc ttaaggtatg
     5281 atcgatatat acgatgtctc tttcaagatc tgcctgttta tacagttctt cattttccgt
     5341 tgttttacga gctgctttat ccaaagcgtc aattcgcttt ttcatctctc ttagttcttt
     5401 cacgtaatgg gtgtaaatat ctaataatgt atacgtgatg atcttttcaa acgagtttaa
     5461 atgatttcca tgctttgtca gcagccgatc cacaaattcg gtttttctat cgatacaagt
     5521 aatcaagaga ttatcggaaa gtacaaaaga tattggcgtt aatttttttt cgatacgatt
     5581 atctgactga tctagattaa tcactaccaa agaaaatgga tgctctagtt tcgtctcata
     5641 aaggcgctcc atccgagaaa cttcttccgg atattcggtt cctacgaaaa tatcatcagg
     5701 aagctcgaat tcttcgattg cttgctcaat ctcttcgggc tttgcactat caagtacgag
     5761 ccaattatat tttttttcat ctgtttctat tacattcttt tcttcatcca caaagtagta
     5821 tctcaatgta tacctccttt ttggactttc tcgaaactat tagctgaaga agcagttctt
     5881 taatttgata ttataatcga gtactgcgat ttcattttct aatgaaaagc tgtcaagctg
     5941 tttgttgaag tccatgttca atcgtttacc gaaataattt tgctctaatt tagatgtata
     6001 gatgtccaat aatggatgct cgattttagt cgcatattct ccaaattccg aaacaaaagg
     6061 aatgatttga aaaaagctgc cttttgcaaa agcacctttg tcccttagaa atggatcgga
     6121 atcattgata agcgcgagtg cgatttttgt attttcccca accttttcaa ttaacgtctc
     6181 tactgcattt ttagacattg taatttccat tcctactccc cctcttcagt tgttgtcttc
     6241 tattatttaa caactacatc tgtttttggt tgaacttttg catattttgg atatttaggg
     6301 aatttagcta cacaacggaa agccgtcatc cctgcatatt tttcgtaata tttcttcgtt
     6361 ggcccttcaa aatccttgct ccacggtttc acatgtcctg aaaaatggat aatgtaagga
     6421 ttgttgcgag tttcttcgta ttcacgttca cctgctgctg ttggatgttt ttttgctttt
     6481 gccatgatat agccttgtgc attccaacgc ggatgaagtg gtaaccaacg gtcatgtaag
     6541 atagcattta atgcatcttg gtcatggaat cgtaattttt ctggattatg ttcgatgaaa
     6601 tctaatactt tttgcgtaat attttcatct aaccattttt tcacattgat cagcatcaaa
     6661 cctgagttga aataacgcat tgatttagct gaaattttca ttttttccag tcgttgatgg
     6721 aacccagcgt cttcaacagc tgctacgatt gaatcaccga agtcaagacg ccataattta
     6781 gagatatctt gtaaagcgat catgtcgcaa tccatatata agatacgttc aacttctgtg
     6841 ccgcggaaca attccggaat cgcaatacga taataagctg tttctggaat acgatcgctg
     6901 attaccacat ttgtaaagaa ttctttattg attttcaaga attccacatc tgagttcatt
     6961 cgtgcgtttt tcactgagaa gcgtaaaagc tgtttacttt caaaatcgat atcatcgtca
     7021 atcacataga accgaacaaa tttagacggg ttgcaattat ctaatactga gacaaataat
     7081 gcagctaaat gtggtacgaa ttttgtgtta caacttgcga ctactgctat ttctttttta
     7141 ttcattgatc tcgcctactt tcgttagagt actgtgtgct aattttttta agtataaatt
     7201 ggtatacgga tgatctttca acgtattcca aggtttatca tgtcctgtaa agtgaacgat
     7261 cgaaggtttt tcatttcctt ctttatactg acgttcatat ttttcatttg gtgctggatg
     7321 acgttcaaaa atcagcgaag tctgcatgtt ccattttgga tgaagttgtt cccaatcttc
     7381 ataaagtaca gcattcaaag catcttggtc gtgatagatg attcgatcgc cattttcact
     7441 gaggtaatgg atagtttttt ctgtaatttc ttttttattc cattgatcaa tgtcaatcac
     7501 catcactcct gaattgaagt acaagtcatc ggaatcgatt cctaatcttt ccaaagcttt
     7561 cacttggcct ggatcgataa tagcgccgat tgtttttcca tttaacggct catcatatag
     7621 ttttacgata tcgtctagta ctaacacatc tgaatctaag taaaggactt tcttataatc
     7681 ctctttagct aataagtttg gtaaagaaat tcttaagtaa gcggtagttg tgatatggtc
     7741 actgactagg aagtcttcgt agatatcttt ttccactgtc aggaattgga tacttgcatt
     7801 acttgaatat ttatttaccg tttcttccaa tccttcttta ctatattctg atagaccatc
     7861 gtcaatcaca taaaatttta tgcgtcttgt tttattgcaa ttttctaatg cagttgcgat
     7921 catcacgctc aagtaaggag cataattttc gtctgaagca gttaccacag gaactacgcc
     7981 gtatctcttc tccatcaaaa tcactccttt ttctaatctt tttctaacct gttctacatc
     8041 tagaatattc ctcatttggt tttaaagcaa aatatatgct ttgttttcat tgaatatttt
     8101 aaaatttttt tatcacaaaa cgcatcggta agcgctaact attcgttttt catggctttt
     8161 tattcaaagg tattgaccaa aaactactat atatagccac ctttttctta agaatgtttt
     8221 ttgaaaaaat tcataatgac aaagcaagtt agtttcattt ttttaaattc tcatttaatc
     8281 ttaaggtaac aggaaaatag atatgtaaaa agaaagtagg aagataaaat gaaactagca
     8341 gcgattgatt tagacggtac cttgcttgat cgaaatggaa agataccaga agaaaatatc
     8401 caagcacttc ggaaatttga tgaaaatggc ggaatcgtaa ccgtcgctac cggaaggaac
     8461 agcatttctg ctaaagatat tttttccgaa ttaggaatcg gtggctattt gatttcttcc
     8521 aatggttcac tgattgctga aatgaaggat ggaaaaatgg cgaaagttct cagacgttca
     8581 aaaatagata gcgatacact gagaaaagcc ttcttctatg ctcaagaagc gaaaatatcc
     8641 atcattgcta gccgagaaac ccaagatgat cagctgacat tcgatgaatc aaatctagtc
     8701 aaagatgacc cttattatga acatttcaat ttacagaacc
//
LOCUS       DACDSY010000123         8494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDSY010000123 DACDSY010000000
VERSION     DACDSY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.125"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IWK44_002287"
     CDS             complement(<1..173)
                     /locus_tag="IWK44_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9550777.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IWK44_002288"
     CDS             complement(593..1714)
                     /locus_tag="IWK44_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP9550778.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IWK44_002289"
     CDS             2042..3451
                     /locus_tag="IWK44_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP9550779.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IWK44_002290"
     CDS             3448..4176
                     /locus_tag="IWK44_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9550780.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IWK44_002291"
     CDS             complement(4304..5215)
                     /locus_tag="IWK44_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9550781.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IWK44_002292"
     CDS             complement(5232..6239)
                     /locus_tag="IWK44_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9550782.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IWK44_002293"
     CDS             complement(6236..8365)
                     /locus_tag="IWK44_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9550783.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IWK44_002294"
     CDS             complement(8370..8477)
                     /locus_tag="IWK44_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550784.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DACDSY010000124         8487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDSY010000124 DACDSY010000000
VERSION     DACDSY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.126"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..460)
                     /locus_tag="IWK44_002295"
     CDS             complement(<1..460)
                     /locus_tag="IWK44_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550785.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFG"
     gene            complement(559..909)
                     /locus_tag="IWK44_002296"
     CDS             complement(559..909)
                     /locus_tag="IWK44_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP9550786.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1066..2595)
                     /gene="ahpF"
                     /locus_tag="IWK44_002297"
     CDS             complement(1066..2595)
                     /gene="ahpF"
                     /locus_tag="IWK44_002297"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP9550787.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2605..3168)
                     /gene="ahpC"
                     /locus_tag="IWK44_002298"
     CDS             complement(2605..3168)
                     /gene="ahpC"
                     /locus_tag="IWK44_002298"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP9550788.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(3312..3974)
                     /locus_tag="IWK44_002299"
     CDS             complement(3312..3974)
                     /locus_tag="IWK44_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9550789.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4158..5804
                     /locus_tag="IWK44_002300"
     CDS             4158..5804
                     /locus_tag="IWK44_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9550790.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5795..5923
                     /locus_tag="IWK44_002301"
     CDS             5795..5923
                     /locus_tag="IWK44_002301"
                     /inference="COORDINATES: protein motif:HMM:NF015727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550791.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDHRKKRLP"
     gene            5890..6954
                     /gene="uxuA"
                     /locus_tag="IWK44_002302"
     CDS             5890..6954
                     /gene="uxuA"
                     /locus_tag="IWK44_002302"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9550792.1"
                     /translation="MDGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPRERIKEL
                     KEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVICYNFMP
                     VFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEPERLAHI
                     KSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLPRIVKNR
                     EDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRNIKFLAN
                     RDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGLYDRALG
                     AVYLNGLWEAIEKGNKSKDN"
     gene            complement(7062..>8487)
                     /locus_tag="IWK44_002303"
     CDS             complement(7062..>8487)
                     /locus_tag="IWK44_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9550793.1"
                     /translation="MSESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPA
                     YCYYRLFGRYCNESVSYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLAL
                     YGLSISFSNNGRKSFSGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFK
                     KEVLDRLNSGMIDKLYFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPN
                     LPICREQRILVNILARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFD
                     LFIPEKEKILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYM
                     DFREQNQHLLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVK
                     IEQIFSNESIKIQNDFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRI
                     SEYIFELKEEALMDQSKKENQVTS"
BASE COUNT         2714 a         1564 c         1550 g         2659 t
ORIGIN      
        1 agccgaatat ttggaataga agcatcaaac ttttgaatgg attgaacaat aagacgaatc
       61 cgccaatcaa caacagaata ctactataaa cactccaact tttagctttt gtattcatgc
      121 tgaagaacaa ctggaacaag ctgttgatga taacagctaa tccaagaaga atcggcagga
      181 tagaaacgat tgcaggtgca aaagcataga cgatcagagc taagatcagg aaaaaaagac
      241 cactgaacag accgatactc caagaacctg ttttcctttt aaaacggtaa gcctcaaaaa
      301 gattcaacaa cccaagcaaa acaagatagg cggtgatcag ataaccgata aaatgaaaaa
      361 cagcagaagg gtttagcaca atcgcaatcc cagaaataat ataaacagca gctcttaata
      421 aggcgtattt ttgaatattt tgtttaatcg tctcaaacat ataagaaaac ctcctttgac
      481 ttattttaac gtgaactagt cattttacca aattaaatgt tacaaaatga gcgaaggaga
      541 agaatatctc tggaaaaatc aacttctgag gatcttttcc atttttttgc cttttgctag
      601 ctcatcgatc attttatcta aataacgaat ttctttcatc aagttatcct cgatgtcttc
      661 tactcgtaca ccacaaacaa cacctgtaat caaggaacgt gaaggattgg ggacaggggc
      721 ttccgcaaaa aattgttcga aagtcacttg tttttgtagt tgatattcta gttctgtttg
      781 ggaataaccc gtcagccaac aaatgatttc atctacttcc tcttttatac gatcttttct
      841 ctccgctttt gctacataca taggataaac gcttgcaaaa cttgtcgtaa aaatacgatt
      901 ttgcgccata atcagtcacc tttctttttt tcaaattatt tactacttat agtatataag
      961 aaaaagagaa gaaaaagcga ttattcaaaa aaacaaaaag gattgtgaca aaattcttgt
     1021 caccatcctt ttttccgctc tctgactttt tacaatcttc atagctcaag tgcgcatcag
     1081 ataatcgaat gctcctaaag ctgcagtagc tcctgaaccc attgaaacaa tgatttgttt
     1141 gtatggacta tctgtacaat caccagctgc gaaaacgccg gggatattcg ttgcgccatt
     1201 tttctcggtc actatttcat ttcttggatt catttcgatc gttccatcta gccaatccgt
     1261 attagggagt aagccgacta gaatgaatac tcctgaaaca gctagtgtat gcatctccat
     1321 cgtacggcga tcgatatagg agagagattc tacctgattc gtaccgctga tttctttcgt
     1381 cgctgcatgt ttgataacag tgacattcgc aagagagact agtttatcct gtaagatttg
     1441 atctgctttt aattctggta agaattctag aacatataca tgttttgcta acccagccaa
     1501 atcgattgct gcttcaacac cagaattacc accaccgata acagcaatct cttttccttt
     1561 aaataaaggg ccgtcacagt gtgggcaata agcaattcct ttatttttaa actctttttc
     1621 gcctggtaca ttgatagaac gccagtgcgc acctgtcgcg atgatggcgg tttttgcttt
     1681 aagtgatata ccgttagcta agccaatatt aataaaatgt tgctctttat ttaaggaaac
     1741 ggcctgttga ttcatcataa tgtcgatagg gtaactacgc acatgacttt ctacttgctg
     1801 catcaatttt ggaccttctg tgtaagaggt accaatcata ttttcaatcc ctagtgtttc
     1861 aacgacttgt ccgccgaaag aatcagcgac aagtcctgta cggattcctt tacgtgctgc
     1921 atagatagct gcactactag ctgctggtcc accgccaatt actaatacat cataaggttc
     1981 tttttcaaac aactcttctt caatcaaagg gccagtgatt ttttcaacaa tttgttcgat
     2041 cgtcatgcgt ccgctactaa attcttctcc atttaaaaac acagttggca ctgccatgat
     2101 ttttttatca tcgatttctt tttggaacat cccaccttca atcatcgtat gagtaatatt
     2161 tggatttaat acagctaata tattcaatgc ctgtacaacg tcggggcaat tatgacaagt
     2221 caagctaaca taactgtcaa aatgcaatgt ttgtgttatt tttttgattt taagactcaa
     2281 agattcactg attttcggtg cccgtccact aatttgtaac atagccagta aaaaagagga
     2341 aaattcatga ccgaggggga taccagcaaa aacgattcca ctttctttgc caactggatt
     2401 gatagaaaaa ctaggtgtgc gcatcgaatc tcttttttcc caagagattt taggagacat
     2461 ttctgctatt tccttcgtaa attcagctag tttttgacta ttttcactat tatccaaact
     2521 aacagaaaaa acaataggag attcaatcag tgctaaatat tgttcgagat tttgtttagt
     2581 ttctttgtct aacatatcca ccacctaaat ctttccaaca agatcaaaac ttggtttcaa
     2641 tgtttcgcca ctttctttcc attttgccgg acaaacttca cctgggtggg tacgtacata
     2701 ttgagcagcg cgaatcttat caatcagtgc actagcgtca cgaccgattc catctgcatt
     2761 gatttccata gcttgaacga ttccgtctgg atcaataata aatgttcctc gctgagctaa
     2821 tccttgctct tcatccaaaa catcaaaaat acgagaaatc tgatgagacg gatcaccgat
     2881 catcacgtat tcgattttac cgatagcatc tgatgtatca tgccatgctt tgtgagtaaa
     2941 gtgggtatcg gtagagcaag aaaagacttc aacacctaaa gatttcaatg tactgtattg
     3001 ttcttgtaag tcttctaatt cagtaggaca aacaaaagta aaatcagctg gatagaagca
     3061 aagaatactc cagttcccca taagatcttt ttcactgact tctaaaaatt ctccttgatg
     3121 ataagcatgt gcattaaatt cttcgatttt tttcccaatc aatgacatag taaaacctcc
     3181 aataataatt atttatttat aattattgta atttagttgg agttgcaaag tcaatggata
     3241 ttttgttatt taataaagat tactcaaaaa cgaaggttaa ttataaaaat agaagaggtc
     3301 gcaagaaaat gctactgctg gaaatactcg ggataacgtt gagtaacagt ttcctgttct
     3361 ttcagcatca tgtgtaagtg caatcccgtt tgaaatcgag cttcttctgg ttttttatca
     3421 actaaagcct tgagtaaatc ataatgttgt tccatgatcg tttcccatgg aagcccctga
     3481 atggtcactc gtaaccagcg gaaacgttct aaatgggtat tatggtcatc taaccagttc
     3541 caaacttctt ttcttcctgc aatttcgaaa caagcttcat gaaataaatt atctgcacgg
     3601 aaaaaagcgc gttcgtcatt tattttgact gctttttctt gttcttctaa aatcgtttct
     3661 agcactattc gtgtttgtgt gtccaatttg gaacaacatt caatcatgat ttgctcttcc
     3721 aactgttctc ggacaaagcg agcattttct gcagaaacaa gattaatttt ggaaacatac
     3781 gtaccacttt gaggtatcgt atacacgagt tcttgctgac gtagttgaat caatgcttcg
     3841 cgaatgggag tccggccgat agcaagatgt tcctccagtg ccttttcaga tattttcctt
     3901 ccaggttcta agtcagcata gataatttgt ttacgaatcg cttgataagc ttgtgattgc
     3961 aagctgcttt tcatctttat tcctccaaaa tcatttctat gaacgtagta taacagaaga
     4021 ggtctgaaaa acaatctggt atgtcacatt aactagtaaa tttgtataaa tagtaaaatt
     4081 ttggttgaca tagcgatttt tattgtataa aattcacttg aatacaaaaa ctaatatgtt
     4141 agtttaggag gggaaaaatg attgatttaa acaactggag gaactttatc cctgaattat
     4201 ctcaaaaaga agtagaaata cctcattttg accgtgcatc attaagagaa aatggacaaa
     4261 aaaatcctgt ctggatccat ttcggtggcg gaaatcttta tcgaggattt catgcagaaa
     4321 tcgctcaacg tttagcggat ctaggcgaat tgtccactgg tgtcattgtt tgtgaaacat
     4381 tcgatgaaga aataattgaa agggcttacc aagattttga tgatttgatt cttgaagtgg
     4441 tcatgccaag cagtggagag ttcgtcaaac gtatattagc tgcgacagct gacagtctgt
     4501 attgtcatcc agattgttct gaacactttg ataagatgat tcgacacttt gaaaatccta
     4561 gtttacaatt agttactttt gctattactg aaaaaggtta tcgattgaaa gatacaaata
     4621 ataaatatat aggaccgatt gcagtcgata taaaaaatgg tccaaagaag ccatcacata
     4681 caatgagtat tcttgctgcg ctactttata aacgtttttt atctggagct tggccgattg
     4741 cacttgtctc tacagataat ttttcacaaa acggtagtca attacgtact tctttgctaa
     4801 cgatcgcaaa agaatggcaa aaggaaggtt atgtacaaaa tacatttatc caatacttat
     4861 cgaatgaaga aattgttgcg tttccatgga cgatgattga tcgtatcacc ccgaatcctt
     4921 ccttgtcagt tagcaaaatg ttggcaaaag agggcttttc aaatatgtcg attttccaaa
     4981 ccgcaaaagg aaataatatt gctccatttg tcaatacaga ggacatccat tatttaatca
     5041 tggaagatca ttttcccaat ggacgtccga aattcgaaaa agctggggta ttcttgactg
     5101 atcgtgcaac ggtagataaa gcagatacga tgaaggtgac gacttgcctt aatcctttgc
     5161 atacgtcttt agctattatg ggttgtttac tggggtatag gagcatagct gaggagatga
     5221 aagatccaga tttatcgctg ctagtttctg aaatcgggta caatgaaggc ctcccagttg
     5281 tggagtctcc aggtatcatt gatccaaaga tctttctgga agaagtaatt actgtgcgct
     5341 tttctaatcc aaatattcct gatatgcctc agcgaatcgc cacggatacc tctcaaaagg
     5401 tggcgattcg ttatggtgag acgattaaaa aatatatgga aatagaagtt aaagacccgc
     5461 agacacttgt attcatacca ttgactctcg ctgcttggct tcgatacctt attggcattg
     5521 atgatcaagg aaaaccattc atcccgagtc cagacccact attgacagac tttcagcata
     5581 agctttccga tattcaactg ggtacgcaag atacatcaca tattcatgag aaaatggctc
     5641 ctattttgtc aaataaagaa atttttggtg tagatcttta ccaagcagag cttggcagaa
     5701 aaatagaaga aatgttcatc aagatggtag caggaccgaa tgccgtacga aaaacattac
     5761 aggattactt acaagaacat ggaggaaatt tatcatggaa atgactttta gatggtacgg
     5821 atcacaggaa gaaaagatta ccttagatga aatcagacag attcctgcgg tcaaagggat
     5881 tgtaggcaca ttgatggatc acaggaagaa aagattacct tagatgaaat cagacagatt
     5941 cctgcggtca aagggattgt aggcacattg atggatatac cagtaggaga ggtatggccc
     6001 agggagagga tcaaagaact aaaagaagaa attgagtctc gtgggttaac actaaaagta
     6061 attgaaagcg tcaatgtgca tgatgccatt aaaattggat taccttcccg tgagcagtat
     6121 atcgaaaact ataagcagac aatccgaaac ttggcagaat atggaataga agtcatctgc
     6181 tataatttta tgcctgtatt tgactgggtg aaatctgacc ttgactaccg cttagaagac
     6241 ggctcatcca cgttagcttt tatatctgat gatattccag atgatccaag agaaatcgtc
     6301 gcgcgcatcg aacattccag caatgaattt gaattgccgg gatgggaacc tgagcgctta
     6361 gctcatatca aatccttgct tgaagcctat gcatcagtag acgaagaaaa acttcgagaa
     6421 aattttgcct attttctcca atcaatcatc ccaacatgtg aagaggtagg ggtcaaaatg
     6481 gctgtacatc cagatgatcc gccatactcg atttttggat tacctcgaat cgtaaagaat
     6541 cgagaagatt tagattggtt gtgccgcata gtagacagcc cttataacgg aatcactctt
     6601 tgtacaggga gtattgcaga agatccagaa aatgatgtat atgggatttt agcagaattt
     6661 tgtcaaagag atcgaatccc atttgcacat gttcgaaata ttaaattttt agctaatcga
     6721 gatttctatg aggcaccgca taaatccgaa tacggttcat tagacatgta ccgcatacta
     6781 aaaacaatgt atgacaacgg atttaacgaa tacatccgtc ctgatcatgg aagaatgatc
     6841 tggaatgaga caggaagacc aggatacggc ttatatgatc gagcattagg tgctgtttat
     6901 ctaaatggat tatgggaagc aatcgaaaaa ggaaacaaaa gtaaagataa ttagtgaaaa
     6961 aaactgtaat tttattatgt aaataagaag gatgttcaaa ttcttgatac ataaatccgg
     7021 aggttgttaa taagcattcg gatttatgta tcttatttgg tctaggaagt aacttgattt
     7081 tcttttttcg attgatccat gagtgcttct tcttttaatt caaagatata ttctgagatc
     7141 ctcataatca cttttttcca gtcttctggt gtgatgattc cttttaaaat atagattttt
     7201 ttgatatgag aaggcaattg tttcaaaggg cagtcgctga taacgatgtt gcagttctcc
     7261 ataaaatcat tttgtatttt gatcgattca tttgaaaaaa tttgttctat cttaacgatc
     7321 agatctttac tgatataaaa atcacgcgta tagttcacac caatcacaat tcgcggctgt
     7381 tttttgtagc gatcatacaa atgtactagg tcttcaacca tccattgcac attttccttt
     7441 tctaaaataa ctggtaacaa gtgttgattt tgttctctga aatccatata aaattgttca
     7501 atggcggttt ggacttcttt caacacgcta tgttcttgat aaagcatgaa agtaggaagg
     7561 ggcgtatttg gttgattgaa gttaagaaat ccattataaa gtttatttcg tgcgaatgcc
     7621 aatgagtaaa gaatcttttc tttttcaggt ataaacaaat caaatacagt acaaaaggac
     7681 tccaccattt ctcttgaata aagaacatga ggaacgtttg ctttttttaa aagtttgtat
     7741 tgctccaagc ttgtttcttc cgattcgatt ttcgtgatag aaagtcgagc taaaatattc
     7801 actaagatgc gttgttcgcg acaaattggc agattaggtc gcaaaatatc tacagaggga
     7861 tcaataaaaa agtcaatata ttcatactct gtgaattctt tttgtacgtc tttcacagaa
     7921 gttgcaggaa aattctcttt acatattttt aatagaaaat ataatttatc tatcataccg
     7981 ctatttagtc ggtcaagtac ttctttttta aaaaaagtat tcatcataaa agccactact
     8041 tgcggatctt catcataaaa ttctgtagca gtacttgaaa agatgttcca ataaatatct
     8101 aatgctgcat attgaatttg tacctctttt ccagaaaaag actttctgcc attattagaa
     8161 aaggagatag acaaaccgta caatgctaaa aatgcattaa tctttttcat tttcgtatac
     8221 aaataagagg cactatagtt tgtttgctga cttaaactga taatcgaatt tgtctgacag
     8281 ctgaacaaag aagtcaagat ctgataactg actgattcgt tgcagtatct tccaaataga
     8341 cgataatagc agtaagccgg atcaattcct tcaaaattca actttaaaaa aggtttttgc
     8401 ttatcaatat gcagttctgt tagattgaga cgctcaaagt cttgttctaa ttctttgatg
     8461 tatagtaaaa aatttgattc agacata
//
LOCUS       DACDSY010000125         8221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDSY010000125 DACDSY010000000
VERSION     DACDSY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.127"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..418)
                     /locus_tag="IWK44_002304"
     CDS             complement(<1..418)
                     /locus_tag="IWK44_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP9550794.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEI"
     gene            complement(548..1258)
                     /gene="budA"
                     /locus_tag="IWK44_002305"
     CDS             complement(548..1258)
                     /gene="budA"
                     /locus_tag="IWK44_002305"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP9550795.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1271..2935)
                     /gene="alsS"
                     /locus_tag="IWK44_002306"
     CDS             complement(1271..2935)
                     /gene="alsS"
                     /locus_tag="IWK44_002306"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP9550796.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3143..3805)
                     /locus_tag="IWK44_002307"
     CDS             complement(3143..3805)
                     /locus_tag="IWK44_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9550797.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3815..4630)
                     /locus_tag="IWK44_002308"
     CDS             complement(3815..4630)
                     /locus_tag="IWK44_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9550798.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4891..5334)
                     /locus_tag="IWK44_002309"
     CDS             complement(4891..5334)
                     /locus_tag="IWK44_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP9550799.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5468..5806)
                     /locus_tag="IWK44_002310"
                     /pseudo
     CDS             complement(5468..5806)
                     /locus_tag="IWK44_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5794..6171)
                     /locus_tag="IWK44_002311"
     CDS             complement(5794..6171)
                     /locus_tag="IWK44_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9550800.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6395..7588
                     /locus_tag="IWK44_002312"
     CDS             6395..7588
                     /locus_tag="IWK44_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9550801.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7754..8176)
                     /locus_tag="IWK44_002313"
     CDS             complement(7754..8176)
                     /locus_tag="IWK44_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9550802.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2406 a         1668 c         1399 g         2748 t
ORIGIN      
        1 cgatttcatc ataccctttc gttactccgc aatcgatatc taaaacaccg attaaattac
       61 cttcttttac cattggcact acgatttctg attgtgcggc tgaatcgcag gaaatgtaat
      121 ttgcatgggt tttgacatca tcaacgatca atgtttcttg tttttcagct gattctccac
      181 aaactccttt tcccatcttg atacgtgtgc aagaaactcg cccttggaac ggtcctaaaa
      241 tcaattcatt accatcgaat aaataatatc ctgcaaagac agtctcaggc aaagtctcat
      301 tcaacaatgc agatgaattt gctaaatttg ctaaccagtt atcttctgct tctaataatc
      361 cttgttgctg taataataaa atttcgtatg catctttttt ctgtttttct gtccacatga
      421 taaaaaccgc tcctttttga tctatggaga agattatagc tcagtttttt tgtaaacgct
      481 aggtttcctc ctcaaaaaaa cgagatacgt caggttaatc accttgaacg catctcgctg
      541 tctttttcta ttctgcttgt tggatttctg ctgctaaatc agcataatca atcgtttcgt
      601 gaacaaaagt cggatcatca actggaaaat gctgtacaag ggacgagatg cggctaatct
      661 caacttttcc ttcagccact tcaaaatcca ttacatgtcc gccaaatgta tgtgtatcat
      721 cgataaaatg caggtgaaat cccgctgcag caactccttg gaataattca ggcgtaaaga
      781 acccgactaa tgttccttgg atattgcttt gttggaattc tggttgagac tctgaaattt
      841 ttgctagtct tctgtaaggt ttttcttgct tcggcatgat acggatgtgc atatttttga
      901 ataatccagt tatcttcact gccaaaaata ggttttcccc ggcttctctt actaatatgt
      961 cttttttcaa ttgttctcca tctgcagttt ttgagacaga aaaactagta tctggcgtaa
     1021 aatctgtaat tgctgcatag ggggtaagtt cacttccatc taattgggca actgagccat
     1081 ctgatcttcc ttgataagct acgccatcta aaaagatgac ttcaccatca agcccatgca
     1141 atgttcccaa tcccagagtt cctttttcta atatatgagc aatcgtttct gttccatcca
     1201 tcaagccggc catcaaagct cctaatgtcc cgtgctggta taagattttt tcagtcatgt
     1261 cgacacttcc ttaatagaat tgatctggta agattgtttc tcccaatttc tcattatcac
     1321 ggtaatcaat cggaatatca ataattacgg gtccttctgt cgcaaatcct tcttctaaag
     1381 ctttttctaa ttcctctaca cttgtagctc ggattccttt agcgccaaaa gcttctgcat
     1441 atttcacaaa atcaactgga ccaaagtcta cacctgacgc ccgctgatat ttcatttttt
     1501 cttggaactc taccatattg tagtgtccat cattccaaat caaatgaacg atattcaatt
     1561 ttttacgtac cgccgtttct aaatcctggg cagagaataa aaatccccca tcgccggaaa
     1621 ctgatacgat ttgtgtattt ggacgcacca atgcagcaga aatcgcccat ggcaatgcaa
     1681 caccaagcgt ttgcatacca ttactgaata acaaatgacg aggttcataa cttctaaagt
     1741 ggcgtgccat ccaaatataa tgactaccta catctacggt aacagtcata tcatctgtta
     1801 cttttgactg aagcgtgtta ataacttcta aaggatgcaa gatacctgct tctccttttg
     1861 attgaacaat gtcccgctcc gttaattttg cttgaagaga tgctaaatat tcttttgcat
     1921 cttctgacac ttgttgaggc tctaatgaac ctgtcaataa atcaagagtg gctgagatat
     1981 caccgatcaa ttctctttca ggctgcataa aaggatcgat ttcagctggc gcttcatcga
     2041 tcacaatgat ccgcgcatct ttttccgcat tccagtttct tgcttcatat tcgattggat
     2101 catagccaat tgcaataact agatcacttc gtttcaaaag catatctcct ggttggtttc
     2161 taaataaacc cacacgacca aagaaatgcg cttctaattc acgtgagatc actccagctg
     2221 cttggaatgt ctctacaaca ggcaatgctg tcttttcaac taattgcctg atggctaacg
     2281 tttctttttc acttgatcct cgcataccta ctaatagtac cggtagtttt gcttctctaa
     2341 tgcgttcagc taggtaacgg atatcatctg cagaagcaga acctaattta ggatcgctca
     2401 atggcttgat gacattacct tggacaggtg catccactac atcttgcggg atactgataa
     2461 agctcgcgcc tttttttgaa ctcttagcca tacgataagc attggcaatc acttctgaaa
     2521 ttgtttctgg atcttggatt tctgcactgt attttgtgat tggctggaac agtgctgcat
     2581 tatccatgct ttgatgggtc aacttcaata gatcgcttcg ttttacttgt ccggcaattg
     2641 ctaaaactgg gtcaccttct gctgtcgctg ttaccaaacc tgtagctaag ttactagcac
     2701 caggaccact tgttgcgata actactcctg gctctcctgt aatacgtccg attgcttgtg
     2761 ccataaatgc agcattttgt tcgtgacgag tcacgattaa ttcaggacct tgatcctcta
     2821 attcattaaa tacaccatcg atttttgcac ctggtatacc gaatacataa tcaacatgat
     2881 gattaatcaa actttctact acagatttcg aaccttgttt cattttttca gccattgaac
     2941 atcgtccttt ttatgcttta tttttaatta ttagttagaa ataatcacaa ataagtgata
     3001 cagtttctaa acctgtttca tacaaaagta tagcacgttt tatttcattt gtttagccat
     3061 atgtttcaaa aaatgaaaaa gaatgacagt ttttacataa aaaaaactgt cattcttaat
     3121 tgaaagtttc aatttataaa gtctacagat ccatagttct catcaattta cgattgttga
     3181 agtaaatcaa accaaatacg atagaaggac ctagtagaat acaccattgg ggctttacga
     3241 aaaagaagag aaacaaactt gtcaatagta aaaaatctag taaactcaca taccattttt
     3301 tcaacgtata gtagaacgct atccgtagaa cttgatttgg tttcgacgct ggatttctca
     3361 cctgaaaata ccagcagttg gtgctaaagg aaactgataa acaagcaagt aaaacaaaca
     3421 acggaatcaa ccattttcca taagaaaaac gtataaaaaa gaaacaatca aaaatcatta
     3481 tcacactaac tatccaagca aatagccaat aaaaaaatcc ctttagccaa aattggcgaa
     3541 acatctttat gaacaaacgt actggtgaaa aactattttc ttttgctaat tcttctgtca
     3601 tacctatcat cacgatgcaa cctggaccaa taaaaagtaa agaaaaaata aaggaaaaga
     3661 cattccttcc atctatcgac aaaaggaacg cagctgctat atacaagata ttgacacacc
     3721 aaaaacaaag gctacttgcc aatgattgat acaaccaata aaagaattcc atataaacat
     3781 ttttgtcaaa acgctgctct ttcataatca cctcctattc cttcattcct gatgaagcga
     3841 tcccttctac aaaatatttt tgcatcgcta aaaatataaa agcaacagga agaacagaaa
     3901 caactgaggc agccatgatt aatgcgtact ctgcatcaaa tagcccaata aacattttca
     3961 accctaactg gattgtttta ttttccgtag aagacaaata aatgaatggc cccatataat
     4021 cgttccatgt attaacgaat gtgatgatcg tcaaactggc aatcgccggt tttgtaagag
     4081 gtaaaatgat cttgcgatag attccccact ctgataagcc atcgatccgc gcactctcac
     4141 ataaagaatc tgggatacta ctgtaatatt gcttcaacaa gaacacacca aaagcattga
     4201 acgcttgtaa tagcaccaac gaccacaacg tatctgttaa acccactttt ctcatcataa
     4261 tgaactgcgg gatcatataa gactgccaag ggacagcaat cgtgccgata tatgctaaaa
     4321 ataaaatatc gcgcccttta aaatttagtt tagaaaaccc gtaagcagca aaactagaag
     4381 taaacaactg aatcaacgtg atcacaacgc ttaaaaaagc tgtattctta aaaaatgtca
     4441 ctaaaggaat acgagaccaa ataaccagat aattctctaa atgccaagtt ttagggatcc
     4501 attgaatcgg aatcgtgaac acttcattat ttgctttgaa tgaagcagaa accatccata
     4561 aaaaaggaat gatcgtcaat aaagctaata caatcagcag aatcataccc aaccattgaa
     4621 taaagggcat gttttttgaa cgaatttttg ctttttttct ttcatttcga acacctccaa
     4681 agttataatc ttcatttctt tgtaatgatt acaatccaga acaacggtta ttatagctga
     4741 ttcatttccg ttcttcttat ttttgtaagc gcttatttaa caagttatgt aaatctcttt
     4801 atttttttga tttaaaaatg aaaaaataca aaagcaagac tgtcatacgt ctcgcttttg
     4861 tattgaaatt tcttcgatta gagatactta ttactgttca aaagcttctt tgaaacggtg
     4921 aatcaattca ttgactacag gtgataaaat ccgattcttt ttccagacta gcatgcagtt
     4981 cgtttcaatc attgggtcaa gcggaacgaa tgtcactcgc tttgaatgat gacttacagc
     5041 accttctatg actaaagcag cacctacctg attctcaatt agcgcaaata cattgaaaat
     5101 caaattgtac gttcctgtga tcagcaactg ctcctttttt atgcctaacc agtcagcaaa
     5161 cattcgctgt atttctggac gctttgagat caaaagaggt acattcgcaa agtcttcttt
     5221 tgttatcacg tctttttttg ctaaaacaga attttctaaa atgagcaatc cccatttttc
     5281 ctttcttggt aacaaaatcg tttcgtattt ctctgtatct actggatcta gcaaaatcgc
     5341 taaatctaac aggctttttt ctagtcactc cgtgatatca tcacttgttc cgctgtggat
     5401 atgaaattga acgctgggat aatcacggat caacttttct agtaatagtg ccaatgtatt
     5461 cgaactatca gcttccacac agccgatcgt aaatcggccg ctgaataatt gtttttttct
     5521 gttctcgaat tcttgagtgg tttgattcgt taaatctaaa atttcttctg ctcggctttt
     5581 ttagaaaata acctgcttct gtcaagatca ttttgcggtt ttcgcgtaca aacagttctg
     5641 ttcctagtta ctcttctaac tccttcagtt gtcggcttaa ggttggttgt gtaagatgta
     5701 gaacttctgc ggcttgagag attgttcctt cttctgcaat tgtccaaaaa tattttagta
     5761 agcgtaactc catagcgatg ctccttgatt tttttacttg atacattcca ttttatttct
     5821 agataacaca cgatcatatt tcttgatttt tccttgtaaa cgttccaaac attcttgcat
     5881 ttcagcaatt tttttcgtca aaagttcact ttcttcaatc aaaagcttac gccgctcaaa
     5941 tacagttgct tctccttcgc tagataggcg aatatattca atcaaagact cgatcgataa
     6001 tccggcattt cgcatacaga tcgtaaagtc gacccatttt aggtcttctt ctgtataatc
     6061 acggattccg ccttttgtcc gattaactgg tggaatcaac ccgattcgtt cataatagcg
     6121 caatgtatcg gccgaaatgc ccttttcttc actgacttgt ttgatattca tctgatcact
     6181 ctttctctta atttcttgcc actttactta tgtattttat ttattctaca actttaagtg
     6241 tactccaagt caatactcat ttctattaaa actaagctgg attatctata tcggttgttt
     6301 gtgtattatt tcaaataaaa aagtcttgtt tttttattct tttttggata tacttttaac
     6361 aatttaggga aattttagga aaaggggtat aaaaatgaca acgtatcagc gaaaggtatt
     6421 agtatcgaca tcggcgggaa ttgctttaga gaatatggat attatgtttt tagctttttc
     6481 attatcttca atgattactt cttttcatat tagtggtacg caagcgggtt tcatttcaac
     6541 gatcactaat ttggggatgc tcattggagg tatttttttt ggcatcatag ctgataaata
     6601 tggacgtgtg aaagtatttt ctcaaacggt ccttctgttt tcgattgctt ctttattgat
     6661 gtattttgct tcaaacatct acttggttta tctctttcgc tttatcgcag gtgtaggagc
     6721 gggaggagaa tatggggcgt gtatgtcctt gatttccgaa agtttttcaa aaaaacaaat
     6781 tggacgagct tcttcggttg ctggtattgg tgcgcaggtc ggtgcggctt tggctgcgat
     6841 tttagctgct gtgatcattc caacgtttgg ttggaagatg ttgtatgtcg taggtgtact
     6901 gccggtgttg atggtcttgt tcattcgtcg cggcttgact gaaccaaagg aattcaaatc
     6961 tgtacaatct gccgaaaaga aaaccaaact tagcagactg tttgaatcaa aacatcttgc
     7021 ttggcaaaca tgtggcctta gtatcatggt aactgtccaa attgccggct attttgggtt
     7081 aatgaattgg ctaccttcaa ttatgcaaaa tcgattaggg ctttcggttt ctgggtcttc
     7141 attgtggatg gtcagtacta ttttaggcat gtctttgggg atgatgactt ttggcataat
     7201 catggataaa ttggggccta aactagcctt ttctttgttt ttaatttgtt catccttatc
     7261 tgtctatcta cttgtgttag cccatagcca gtggagtttg gtagcagccg ctgtgatagt
     7321 cggctacttt atcaatggca tgtatggtgg atatggcgca gtgattagta gtttgtatcc
     7381 aacagaaatc cgcgcgacgg cgaataattt cgttatgaac cttggacgag ctgttggtgg
     7441 tttttcttct atcgtcatcg gtttcttgat ggatcattat agtttaactg ccgtaatctt
     7501 gtttttgagc ggtatttacc tagtcagtct gctggtcttg acaactctta ctggtgttac
     7561 agcattaagg gaaagcttgc agcattgata gttgagtctg taaatgacaa gaaagaggtg
     7621 tggaaaaagc ttgcatctgc ggtaataaga ccagccaaat caaaaacatg agacactgtt
     7681 tccgtttggc tggtcgtatt acttgatgca gggcagcttt ttcaactgct aatttccttc
     7741 tatctggcgg cgactattct ttgacgtccg tcatttcaaa gtactttact aataacttag
     7801 cataacgaaa aactgtggat acactcgtat attgagcgtc tgcataatct tccaaatttt
     7861 tctcaggatg ttgatacatg aattcaatca aatgataatt atatgatttt ctgtagaaat
     7921 gaagataaat aatgtagatt gggaagttct cctgcttttc catagaaaag cgccgattat
     7981 tctcattctt caaaatgacc ttatcaggat attgttggaa caattcttct aaatctgtta
     8041 gataattcaa gatagactta cttgatacct tcaattcgtt ttgtagttct tctattgaac
     8101 accaacgttc tgtttcaagc agtaaatgaa tcattttaat tttcttttga gaaacttttg
     8161 ttaaaaaagt ctgcatattt tctcctcctg tatctacttt actatactac atacaagaaa
     8221 a
//
LOCUS       DACDSY010000126         8084 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDSY010000126 DACDSY010000000
VERSION     DACDSY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.128"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..479
                     /locus_tag="IWK44_002314"
     CDS             <1..479
                     /locus_tag="IWK44_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550803.1"
                     /translation="GCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            492..2510
                     /locus_tag="IWK44_002315"
     CDS             492..2510
                     /locus_tag="IWK44_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP9550804.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2570..3286)
                     /locus_tag="IWK44_002316"
     CDS             complement(2570..3286)
                     /locus_tag="IWK44_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9550805.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(3513..4055)
                     /locus_tag="IWK44_002317"
     CDS             complement(3513..4055)
                     /locus_tag="IWK44_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9550806.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(4060..4287)
                     /locus_tag="IWK44_002318"
     CDS             complement(4060..4287)
                     /locus_tag="IWK44_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP9550807.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(4366..6246)
                     /locus_tag="IWK44_002319"
     CDS             complement(4366..6246)
                     /locus_tag="IWK44_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9550808.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(6435..7049)
                     /locus_tag="IWK44_002320"
     CDS             complement(6435..7049)
                     /locus_tag="IWK44_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9550809.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            7191..7751
                     /locus_tag="IWK44_002321"
     CDS             7191..7751
                     /locus_tag="IWK44_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9550810.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         2455 a         1587 c         1326 g         2716 t
ORIGIN      
        1 ttggctgctc tactctattt aataaagacc cgtttgcatt acagaatgga cagagtattt
       61 tatggatttt ttatttattt ttaatgggtt atggtttgaa agaatggaac tggaaagaaa
      121 aggtccgcta taagcttcct caagtattta tcagcttgat tgttttattc ggtttgatta
      181 ttttgatgac acaaatttca ctcatcatca gaggaaatac agatacagcg ctgaggtttt
      241 ctaaaccata ttctctcttc gcaatgtatt atactttctc ttcgttttta tttttagaaa
      301 tgctatctga taaattaggt ataaaaggaa gaggaaattt tcttgctgta tttttgattg
      361 tcactcaagt tatatgtaat ttccctttag tttcttatcg cgtatctaca tttatgaaac
      421 gtgaatttcc aaactcaggg aaggcatggc tgataaatat cgggcagttt tttatttgat
      481 atttattagc agtgtccctc ctagttatct ttttatttta tatacaaaaa atccgtttca
      541 ttcatcgttt catcgaatac ttcgcttttg attcgattga acaattaact cagcgttgga
      601 aaaaaagaat tcattggctt tttgttcata aaagttattt tttagttgca gcattttttt
      661 actgctttac cttcgtgcag attttcgttt tagaaccaaa agaaggttgg aaagaaacta
      721 tccaggttgc cttaaaaata tttactcagc gacaagcacc catgatcctg acaatcatca
      781 ttattatggg atttttcttc ttactattgt tgatttccaa tcgtttttgg tacgccttga
      841 ctgctacctt gatcattaac ttactgctca ctatttcgac agtaatcaaa atggaaatgc
      901 gggaagagcc tgtctttcct tcagatttaa aaatgctgac cggattgtca gaactattat
      961 cgatggttag cccagtctta ttaatcgtgg gcggattgat ccttctccta ttattgatta
     1021 ctagtattat tgtacaacgt agactacaaa aacagtattc cttgaagatt cattggaaac
     1081 gtcgatttat cagtatcgct gtattattag gatgtttttc gggtgtcttt ttcattaatc
     1141 acaaaaattc gccttctttc ttgctgttta atttatttaa agtaaacaag actttcttta
     1201 accagcaagg cgccgtaaaa gataacggac cagtcattca attcctaaac aatatcgaca
     1261 ttaaaatcat ggaagaacct gctggctatt cgaaagaaaa aattaatgga ataatgaaaa
     1321 agtacaacac aaaggcagat gaaatcaacc aaacaagaag tgactggtta aacaatcaga
     1381 cgatcgttct caatttaagt gaaagcttca gtgatcctgc acgtgtccct aatctgacag
     1441 tacctacaga ccctattcct tacatcaaaa gcattataac agaaggaaca ggaggattga
     1501 tgttatccgt cggttatggt ggcggaacag ctaacatgga atgggaagga ctgacaggct
     1561 tgagtatcag taacctgtct gcttcgttag ttacacctta tacccagcta gtggagcgtc
     1621 agaaaataag tccaaatatt accgatctat tcgatgaaaa aatagccatc catcctttta
     1681 cagctgctct ttatcgcaga aaacaagtgt ttgataaatt tggttttgac aggttctatt
     1741 atttagacag tccggataaa ttaacttata cggataaaat agaacaaagc ccacgcatat
     1801 cggatgattc agcatacaaa gaaaccttga aagctttaca aagcaatacg gaaacgtcac
     1861 aattcatcca attatccact atgcaaaacc atatgcctta tgatgattac tatagtgaat
     1921 tgaactacac tgctgaagga actgctgtca ttcctaatag aagaaaagaa ttacaaactt
     1981 atatgcaagg ccttcactat accgatacag cagtaaaaaa attcatccaa gaaatcgaca
     2041 aaatcgataa accgattacc tttgtttttt atggtgacca tttacccgca atctatagtg
     2101 ggaatgattc gaaaaaatat ggattagaac agcaccagac agattatttc atttataaca
     2161 acaagtatag tcgtgaacaa gcagctacgt taaataaaga agttgttgct cctaataact
     2221 ttggtgctat ggctcttgag catgcgaata tcaaagtaac tccttactat gcgttgttga
     2281 cagaagtatc taatcatcta cctgcattaa caattgatcc tacagaaagc ttgtctaatc
     2341 gttttaatgg aaaacaggtc tttgttacag aggatagtca aattatccaa gaaaaaaatt
     2401 taactgagaa acaaaaagaa attctagaag attatcgtct gatacaatat gatcttgttg
     2461 caggtaacca gtacagtgca acatgggcga cacaaaaaat aaaagactaa caaataaact
     2521 gagcaagggg ttgtgataaa agacatgtca caaccccttg tttatctcat taatttgctt
     2581 gtccataaac gatttgatta aagccatttt tcaattcatt attcgctgtc tcgttgaagt
     2641 aaagataatt atcgggattc ccctgatttt gttgtgtcac caatactttt ggctgatttt
     2701 gctgaaaccc gaatagatag acgtgctttt tcagatatgg ttctgtctct gcatcggaat
     2761 agacggctgt gatttgatag tctgcttgac ctgtcccact ttctgaccat tctgcagaaa
     2821 caggcactcc accaatagcc atcttccact ccccgttcaa aatagcttga gggactttaa
     2881 gaccatataa gtccacactc atttgattcg tataactttt gtattgttgt cccattgttt
     2941 ggccccattt ggtcataaat gcctctaatt gtgcggattt gtctgcattc cacagtgttt
     3001 gttcaacttg ttcgctgctt tctgaagagg aagatgaagc ctctacagaa gaacttgaag
     3061 tagatgtttg agttgtttca gtcgttttgg aagactctga cgaagtagag gtatctgctg
     3121 tttctgtact tgctgcagtt gacttgctct ctgtgctagt tgttgtagta gttgcaactg
     3181 ttgctttcgc tgtttctttt gttgattctt ttttcgattc attcccgttg gtacaaccag
     3241 ctaacaaaaa agctagacca attgttacta aaatcctttt tttcaattaa aaaactcctt
     3301 tcgattactc ccacttcttt attctatcaa ataagcaatg tgattttatt gatttttcgt
     3361 attttctcat caatctttca aatcttctaa aaactgagaa gcacccgctg atgttgtctg
     3421 taatacatca gcgggtgctt cttttatttt aatcttcgtg attttccaat ttacctcacg
     3481 ctttatattt gtatttgaag aaacatagac ttttagtcat catcgtcttc ctcatccaaa
     3541 tcttgtcgga cagtttttcc ttggagtctt tgtacttggt agatgatttc tttattgaaa
     3601 cttaaaagtt caacaagcaa ttgttgcaag ccatattttt cttctttttc agctaatttg
     3661 attgcccgtg tgataaagac attttggaaa tcaaaatctt ttgcaaagtt ttccaacatt
     3721 gttgaggctg gtaaatattt gttttgacca tgttcgtaaa tttttccata acgcatgact
     3781 tcttctaaag tagacggaac gatttcattt tcaatcacta actcatgaga gattttattg
     3841 aaccatctcg cttcttgatc agcatattct gaaaaaaggg tacttaattc ttgcgaagat
     3901 gtacccaaaa catacaattg tgcttggatc aatttcattt cttctaattt aaaatttgct
     3961 aatacatgcc ccaccatggc gccagcagtt ggtttatgat ggtcgatatc tgcttgtttt
     4021 gtttcttgaa caaatttttc ttccactata ttcattgatt tacgcctctt ttacttttgc
     4081 tgattggact tcataaccta atttttccac aactgcagct aattgttcag ggtggacttc
     4141 atctggatca aagtttgctt tgattttgct cgcattaaac aaaactttcg tattgcttac
     4201 cccaggctgt tgagaaaccg catgttcgat tttttgcata cagcttggac aagctagtgt
     4261 atctaattgc ataataattt ttttcatcgt agttcttcct tcctttttct tgatagtctt
     4321 attctagatt acagcttcca taaaaaaatt gattcttatc aagttttacg atttttcttc
     4381 tttgctttcg tgtccaaaat acaataaacg catcgcgtta aagataacga tcaagatact
     4441 agcttcgtga acgaacatcc cactagccat gtaaataaac ccggcaaata agccgttgag
     4501 taaaaataag acgacaccaa tggcaatcac tacattttca cgggtattta aaacagtttt
     4561 cttagccaag cggtaggcat gaaccaacga ttcaaaatta gattgcatca agacaacgtc
     4621 actcgtttcg atagcaacat ccgttcctga tcccatagca atcccgatat cagccgcagc
     4681 caaagaggga gaatcattga ttccatctcc aataaaagca acgcgcaatc cttgttcttg
     4741 gtattttttc acaaattgaa ccttttcgtc tggtaataat tcagcatgga cttcatcaat
     4801 gcctaacata tcagctacat aatcagcagt catctgatta tcacctgtca gcatcactaa
     4861 ctgttttgct cctgcttgtt tcaacttagc taattgttca gcgacttctg gtcgaatcac
     4921 atctgacaca cctatgattt gagtgatttt tttgtcgatt gctacaataa tgaccgagct
     4981 gcctgtttgt tgtaattgat gtaaatcttg tacttgcttt ggtgtgagag aaagtttgtg
     5041 agcggcaatc aatttggtgt tgcccgcaca aactgtatga ccatcaatct cagctgtgat
     5101 tccttgtccc ttgattgtct gattatcttt gactgctaat tgttgaaaat caatgtcttt
     5161 ctcctgagca taatggatca cggcgcgccc cagaggatga tcggataacg tttctaatcg
     5221 agccactaaa gctaagctgt ccccttcatc ctctgtatag ttcttgatag tggatacagc
     5281 agtagttcct ttggtcaatg taccagtttt atcaaagaca aaagtatcga ttttggaaaa
     5341 agtatccatt gcttcaccgc ctttgactaa gatgccccgt ttcgctccat taccgattcc
     5401 tgctacattc gaaacaggcg caccaatgac taaagcgcca ggacaaccaa gaaccaaaac
     5461 agtgattgct aaacgaaaat cacgactaat cagaccaaca accaacgcaa taagtaaaac
     5521 ggttggagta taatactgag aaaatcgatc aatgaatttt tccgcatggg atttggtatc
     5581 ttgtgcttct tctactaatt cgataatttt ggcaaaggtc gtatcatctc cgaccttagt
     5641 tgcttcaatc gtcacgtagc catctgctaa aatcgttcca gagaagactt catctccact
     5701 gattttgacg acttgtcgtg attcaccagt cactgcggct tcatccgcat accctttccc
     5761 gtcaatgact cgtccgtcca caggaatagc tccaccagtt tttactaaga cgatatcgcc
     5821 ttcttccacg tcatcgattt ccacttcttc agtcgttcca tcttcttgga caacactagc
     5881 agtagatggg gccatctcgg tcaaagcctt gattgactcc cgcgttttgg tcaatgtttt
     5941 ttgttccaga tagctaccaa acaaaaacaa gaatgtaacg atagctgact ccttgtattc
     6001 tccgatcata aaggcaccaa gcacagcaat agtaacgagt aaatcaattg agataacttt
     6061 gactttcact gcctgatacg catggatcat aataggtatg accgcaataa tagaagctat
     6121 ggctagagca atgttataga acaagacaaa accagtaaat tttccgataa aaccaaaaat
     6181 aatcaacaca gctgataagg cggtgatatg atttcgatgc tcttggatcc acttttgtaa
     6241 tttcatcttt ctccctcttt tcctttcatt cttacgagac taagtataga tacttatcca
     6301 tcataaaaaa ttgaccacta tcaagtttag tcaaaaaggc ggttttcttt tggtagaata
     6361 cactacaaaa aatagttagc aagaagactc actaaaagtg atcttgccaa ctattgtcgt
     6421 ttctatcgaa attttcattg aatgattttc ctgtacagca tacatcggag atctatttct
     6481 ttatctaaga tttttatctt gctaattttt tcctttagtc tttcaaatcc acgtcgttca
     6541 tagaattgat acgtacagcc actatctgta aaaaggaata tttcttttcc tttctctctt
     6601 ttttcaaatt ctgttagcaa cttggtaccg atgccttttg ttttcaaatc aggatttgca
     6661 gctaaaaaaa tgatctctcc atctggcgaa tgcttcttta aatatgctgc aagcatttgt
     6721 tcatttgctg tctcgtaagc tcctttactt tctttagcaa atatattttg taaaaaatcg
     6781 aatatcttaa catatatttg ctgtccaaaa gaacgatgtt tctttttttc tccttttatc
     6841 tccgccagca aaacacctgc caactcgtcc tctgcataca aagcaagaat ttgagttgct
     6901 cgattcaact ccatatacaa aaaatatctt ccgtaaagat tcagcagaag ttcattttcc
     6961 atatatctct caaagttcat tccttcaaca gcaaaataga tagctttttt gtaatctttc
     7021 ttttctaagg gtttaaaagt tagtttcatc atttcgcctc ctcaacttca atgattgtgt
     7081 tatacttaca ttataataga cacagtgtct actatctata atcaatcatc tataagtttg
     7141 tcgattaagc gacagctttg ctttgatatg tcggataagg agataggtaa atgaaaaaga
     7201 cagatagacg cgtcaaaaaa acagaaaaag ctttggctga aacactatcc acattattag
     7261 taaataaaaa gattcaagca atcactatac gcgaactaac agaaacggca gatgtacatc
     7321 gttcgacttt ttatacacat tataaggata tctatgatct atatgatcaa ttagaaagca
     7381 acttttttcg ggatctaaat gaaattctct cttatgatcc agcccatagc tatgaggaac
     7441 tatacactcg cttgattgac tatgtttacg caaatacttt tttatatcgc ttatttatcc
     7501 tcaaccatgg tgatatttcg tttcagaaga aagttaaaca agttattgaa gaaaactatc
     7561 tgaacatatg gctatatgaa gacaagaaaa cgattatcac agaagaaatg agattcttta
     7621 ctacgtacca cgtccaaggt tgtctatccg ttctcaatcg gtgggtagaa agcaattaca
     7681 agtactccaa aaaagaatta ttagaactct tacagaaact gaatgatcac atagagaaaa
     7741 tcatgccttg aataaaaaca tactaagctt agttaagaag aaccgtaacc ttctcgtata
     7801 acaactgaat cttatttagc caaattgttt actgaagatt ttttgagtaa aatttctata
     7861 attcctactt attgaaaagt agtttgtagt gctgcttctc cctctctaag tgatatatct
     7921 tgttgtacca atcaaattca cctcatcatt aataaattac ttttaggcta atttattaca
     7981 acttataggt tgaagctgta accaatttat agtcatatta tatttttatc atccagttta
     8041 ttgaattctt ctcgtttacc tcaattcatt ttcaagggga atca
//
LOCUS       DACDSY010000127         8064 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDSY010000127 DACDSY010000000
VERSION     DACDSY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.129"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1454)
                     /locus_tag="IWK44_002322"
     CDS             complement(<1..1454)
                     /locus_tag="IWK44_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9550811.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIA"
     gene            complement(1447..2898)
                     /locus_tag="IWK44_002323"
     CDS             complement(1447..2898)
                     /locus_tag="IWK44_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9550812.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(2912..3928)
                     /locus_tag="IWK44_002324"
     CDS             complement(2912..3928)
                     /locus_tag="IWK44_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9550813.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            4148..5236
                     /locus_tag="IWK44_002325"
     CDS             4148..5236
                     /locus_tag="IWK44_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9550814.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            5290..6816
                     /locus_tag="IWK44_002326"
     CDS             5290..6816
                     /locus_tag="IWK44_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP9550815.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            6818..8023
                     /locus_tag="IWK44_002327"
     CDS             6818..8023
                     /locus_tag="IWK44_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9550816.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
BASE COUNT         2473 a         1396 c         1777 g         2418 t
ORIGIN      
        1 aaagcaatat ctgaaatacg atagtctgat tgctgcaata atttttttgc taattcgatt
       61 ctttgttctg tcagataatc gatgaatgtt tggttaaacg attgagaaaa aatcgtactg
      121 aaatgagagg gacttaatgc aacttgttga gcgacccaag ttaaggacat atctggatcg
      181 gtaaaatttt ctttgataaa atccaatgca tggttcaaaa ttggctgata tttgttcctt
      241 gaaggctgtg agggcagagc agttaagaaa gaaatgaaca tataaactgt cgaacggtaa
      301 agcgagtcgt cggcgctgat tgctaagcgt tgactggtgg atgtgcaatg aaccatttct
      361 tgcgggattt tcttttcttt caggcagaca tgctggagta gcttagtcaa gacaaataaa
      421 cgatataatc gtgttcgagt tggtgtgcct tgtggtttcg tcaagtgttc gatgtactta
      481 gatacttcgt ttttcgataa tgaagacaaa tcaagtgaga ggtcaaagta aaagacagaa
      541 tcaactggaa gtttcgcttc ttgaatcaaa tcctcatagc tgataatttg ttctttaagg
      601 gaactggcgg tttcaagtat catctgcgtg atctcaaaag atgcaggtaa ttcgctgatt
      661 ctttctgcga caggaccaat ggcgacagaa aaattatttt tttgtccctt ttctaattca
      721 tggaccaacg tttgggctac ctgataactg gtctcgatca cattttcttc aagagaatca
      781 actattaaaa atttgatgaa tcgagaggaa acagcagaga agatcacttg ttgattctcc
      841 ctaaataaaa acttcaaata atcactgaga tgagcgtaat cttcaaagtc tgtactgact
      901 tggtttgctg ctaataatac ggtcattttt ttccctgcta aagagcgatt gaaagattca
      961 gcaagtttca atgtttcact gatcgtaagt gagcctttga acaaaccgtt caagaaatga
     1021 ttttttttca attcgaaaac aagatcagaa gaagtgtgcg tttctttttg ctgcaaatct
     1081 aaagcagtta ctgctttttc tattgcttgt ttcaattcgc tacttttaat cggtttaagc
     1141 agataatcat ctgcctgtac ctggattgct cctttcatat aatcgaaatc gtcgaatcca
     1201 ctaataaata tgagacgagt ccaaggaagt agtttccttg cttcttttga aaaagtcaac
     1261 ccgtccatga aaggcatttg gatatcagtc agaatgatgt cgggctgtaa gtcgataatt
     1321 gaagacagtg ccatctcacc atctccagct tcgccgacat aagtgattgg ataataagaa
     1381 ccaaggacag ttatttgttc ccgcaagtta ttgcgaatga ggtattcgtc ttcgatgata
     1441 aaaactttat acatgtttgt ttcctccaat tgttttggga gatagaggga tcatcagtgt
     1501 gacagttgtt ccttctcggt atttgctgtc aatcgtgata ggtttttgat catttccgta
     1561 ataaagaagg agacgcttgt ttacgttgta aagtccgtag ccgatactga aatctgaatc
     1621 gatccctttg gctaactctt tacgtatttc tgccagtttt tctgcaggga tgccaatccc
     1681 gttgtctata actttgaatt ggatcatctc atcaattttg catgcggtaa tacgaataaa
     1741 tcctttgtga cggacgtact tcgttccatg gtatacagca ttttcaatca atggctgcaa
     1801 gatcattttt aaaatagaaa caggaaaaag tgtttctggt atatcgatct tatagtcaag
     1861 catatggccg tatcttattt ttaagatgac tagataatca ttgatatggc taatttcttt
     1921 ttcaagagga atccagtctt gtcctttgct taaagacgta cgaaaaaagc cggaaagtgc
     1981 atgggtcata gtcagagctt cttcaaagca gtcttgttcg atcaaagaaa taatggcatc
     2041 caaactgtta taaataaaat ggggagtaat ctgagcttgc aatacacgaa cttcactttg
     2101 tgctaaatgg tattgtttca aagcgttctc ccgtaaagac tggtttagat cttccgccat
     2161 tttattcatg ctagcggtaa gtgtttgtaa ttccagtgtt tccggcaggg ttaaacgaaa
     2221 gccaaggttc cctgaagaaa gttgttctga catctcgatc agctgattga tcggttcttg
     2281 gacctttctc tctaaaaagc gacgattctt tttcgcatag aaaaaaatgg tactaagaac
     2341 tccaagttcc actattgtta atagaatgag cgagcggaca agtgtcgtat tttttttgct
     2401 tgctaagtcg atctccacac gaacaaaatc ttgtaagata tctgacagca gttgagtgac
     2461 agaatctacc tgattgatga tctcttcatt ggtatccata aaatcttttg ccaagatatt
     2521 attgatcagc tcgttttgat aatcttccag tgtatcgatc gtacgtagcg atacatctaa
     2581 cgtactaatt tctttaagtg tatttgtatt atttttgatc tctcgaatat cattcctaag
     2641 ttcttctagg atattttcct cattgaactc atctacggtt acttggccag aaatcaagtc
     2701 ccacatactt tccaaaactt tttgatcaac cttcaaagca attgcatctg ctttttggat
     2761 atttgcgatc gtctgttgat aaatcaaaag ctgttgcgaa tataacagta tgctcagtag
     2821 caaagggata acagcaaata gaagcatggc tttgtttgct tgggcaaaaa tctgttttat
     2881 gcttaaattt tttgtcatga tacttctcca gtcaataatt tctggttggg atgttagcta
     2941 tattctgctg ggaaaagaca gtctcggaaa taaaaatatc tttaggtaat gtttttcctg
     3001 tttgaatatt tccaaaataa cgagtaacgg tattggctac ataccagccg acatcaggtt
     3061 tacactcgac tacacaattg acgattcctg agcgtagctt ctcgatcata tcttcttgtc
     3121 catcaatagt tacaatgatc aaatcttctc caggtttgat tgatgtttct tttatcgcag
     3181 ctaaagcacc gtgagtcatt tcatcactat ggctgtataa tacatcgatt tcggataaat
     3241 catttgtttc taaatatcgt ttcatctggt cttttccttt catccgaata aaatcaccga
     3301 caattcggtg aacaatagtc atcggaggtt tatggtcgat ggttccatct ttttttaggg
     3361 ttggtttacg attgattgat tccaaaaatc cttcggagcg tagcttggtc ggtgtggagt
     3421 tctctgaacc agcaagttct aggattctga tttcctgttt ctttgaattt gtgaagtgat
     3481 tgctgacaaa taaacccgcc cgatttcctt ctgctttgaa actggggcca atgtgggtga
     3541 cgaaaagctc gggatcattc gtttgaatat gtctgtcgac aagtatgaca gggatccctg
     3601 ctcgctttgc ttcttttaat acagagtccc acccggattc ttgcaaagga gtaaaaatga
     3661 tcatatccac tttataggca atgaacgtcc gtatatcttg gatttgccgt tcctgattca
     3721 tgtagccatt tcggtagaga acttggtaat cttctttttc aagcgcagtg ataattgatt
     3781 cagtatgctt tttccgccag ctactctccg ttcctgattg cgaaaaacca acgactagtg
     3841 ggtcaggtgc ttcctttgta ttaggtgtac accccatcat ttggtaagcg attacaacaa
     3901 gggctaaaaa aaataaaaca cgtttcactt taaatcactc catctggatt tgttagtact
     3961 atcttatcag ataaaaggga aaatcaaagt caaaaggaat caatcgaaaa aaatccaaga
     4021 aacttaaaaa aatccaagac aaaaaaagta cacctaaaaa atttaaagta aatcccataa
     4081 aaaaatcaat gttaacgctt tctttattag ctatactcgg tgtatcaatt gaaatggggg
     4141 ataaaaaatg aaaaacaaat ttagaaagat tgttgggact atcgctattt tgaccgcaag
     4201 cattctatta agtgcgtgtg gcaattcagg atctgcagat gatagtacgg ggtatgtagg
     4261 aatcgctatg ccgaccaaat cagcagaaag atggatcgct gatgggaaca acatggtctc
     4321 ggaattagaa aaactgggtt ataaaacaga tcttcaatat ggagaagata aagtagaaaa
     4381 tcaagtagca caaatagaaa atatgattac aaaaggggta gatacacttg tgattgcttc
     4441 tatcgatggt tcggctttaa ctgatgtgtt agctaaagca aaagaagcag atatcaaggt
     4501 gatcgcctat gatcgtttac tgatgaattc agaaaatgtc gattattacg cgacttttga
     4561 taattttgga gtaggtgtat cgcaagcagc ttatattgaa gagcacttag gattgaaaga
     4621 aggaaaagga ccattcacga ttgaattatt tggtggctcg cctgacgaca acaacgcact
     4681 aatcaattac aacggagtga tgtctgtttt acaaccatat atggacaacg gtcagttggt
     4741 cgtgccatct ggacagacga gtttcagtca aatcgctacg cttcgctggg atggttctac
     4801 ggcgcaagca aggatggata atctgttaag cgcaaattat actgaccaaa cattggatgc
     4861 tgttttgtcg ccatatgatc caataagttt aggaatcatt tcgtcattaa aaggcgtggg
     4921 gtatgggagt gaaagtaaac ctttacctgt gattactgga caagatgcga cagttgctgg
     4981 cgttaaatcg atcattgccg gggaacaaac acaaacgatc tttaaagaca cacgtatctt
     5041 agcaaaaaat acgattgaaa tgatcaaagc aatcagtgat ggagaagagg ttccggtcaa
     5101 tgatactgaa acttatgata atggggtgaa aactgtccct acgtatttag caaatacagt
     5161 atctgtcgat aaagataatt atcaagcaga gctgatcgac accgattatt ataaagaatc
     5221 cgatctgaaa aattaagagg aaccaaacaa ggaactaaag agatctaagt cagagagagg
     5281 ataagaatta tggcggattg tattttaaaa atggcagata tcaccaaaaa attttctgga
     5341 atccgagcgt tagattgcgt gaatctgatg attgaacgtg gggaaatcca tgcattgtgc
     5401 ggagaaaacg gagcgggaaa atcaacattg atgaatgtct tgagcggctt gtatccatat
     5461 ggctcctatg acggggaaat cttttatgat ggaaagctgt gtcaatttaa agatttgaaa
     5521 gatagtgaag aaaaaggcat cgtcatcatt catcaagaat tagcactaag tccttatctt
     5581 tcaataaaag agaatatttt tctcggaaat gaacaagcag aaagaggcgt gattgattgg
     5641 gaactgacag aaaagaaaac atatgaactg ctgaataaag tcggtttgtc tatcgacccg
     5701 aatcttctag tatcccagat tggcgtaggg caacagcaac tagttgaaat agctaaagca
     5761 ttatcaaaat ccgtccgtct gttgattttg gatgagccta ccgctgcatt gaatgaagaa
     5821 gaaagcgcga atttgctgag attgatccga gagttgaaag agcaaggtgt aacctccatc
     5881 attatttcac ataagctgaa tgaaatcgtg gatatcgcag atcgaatcac cattttacgg
     5941 gatggacaga cgattgagac actagaaaat gaaatgatca gcgaagagca tattattcgc
     6001 gggatggtgg gcagagattt agttgatcgt tatcctgaaa gaaaaccgga tattggagaa
     6061 gtttatttta aagtagaaga ctggacggtc tatcaccctc ttgatcacga gcgaatcata
     6121 aacgaccaca tcaacttgca tttgaaaaaa ggagagattg taggaatagc tggactgatg
     6181 ggagcgggca gaactgaatt cgctatgagt ctatttggac attcttatgg aagcaagata
     6241 tccggacgta tattaaagga aggggcagag atatcagtta agaccgttcc ttcagccatt
     6301 gcaaacggac tcgcttatgt ctcggaggac cgaaaatctt taggactgaa tctgctgatg
     6361 gatattcggg aaaacgtgac gctgagcagt ttggataaaa taagtcaaaa aggagtatta
     6421 gataaagaaa aagaagtaac tgaggcagaa aatttccgga aaaagatgcg tataaaaaca
     6481 aactccatcc atcaaattgt cagtagtctt agtggtggga atcaacaaaa agtcgtttta
     6541 tccaagtggc tgatgacaca acccgatatt ctttttttgg atgagccaac ccgtgggata
     6601 gatgttgggg caaaatatga gatctacacg attatcgaag aaatggcagc aaatggcaaa
     6661 gctgtctgtc ttatctcttc tgaacttcct gaaatattag ggatgtgtga tcgaatctat
     6721 acgatgaacg aaggacgtat tacaggagaa gtctcgcgag aagatgcaga tcaggaagtc
     6781 ttgatgaaat taatgacgaa agaagaggtg ggttaagttg gaacaggcaa aaaatcttac
     6841 ggataaaaaa gagaaaaaac taaatttgaa aagtagagca gctgatcttt ttggtcgcta
     6901 cagcatggtg atcattttag cgggactact tattgcgttt caattgatga cggatggtat
     6961 tttctggcgt ccattgaata taactaacct tgttctgcaa aatagccata tattagtact
     7021 agctgcaggg atgttattag ttgtgctctt aggtcatgtg gatttgtcgg tagggtcagt
     7081 gatggcgttt gttggtgcaa ttgcgggcat gatgatggtc aatcgccaaa tgaacccgtg
     7141 gatgacagtt atcgcatgtc ttgcagtagg cgggctgatc ggcgcatggc aaggtttttg
     7201 ggtagcttat gcagggatac cggcgtttat cgtgacttta gcaggattgc tgatgtttcg
     7261 tggactgaca caagttgttc taggaggcca atctctagca ccttttccag ctgttttcca
     7321 aaaaatgtct acgggttatt taccagactt aagcgaagga agcagccttc acttcttgac
     7381 attgatttta ggaatgatca tcacgatcgt gttagttgtt ggtcaatggc gaacaagaaa
     7441 tcgtcggaaa aatcatttat ttgaagtgga atctccaaca gctttcttgg taaaggcggt
     7501 atttacagca gtcgttgtac taggaataac ttatatcttt gcctcttatc aagggtatcc
     7561 gattattttg atcattcttg gtttgatcgt tgcagcatat gggtttttga caaataaaac
     7621 agtagcaggt cgacagattt atgcgacggg cggaaatcga aaagcggctg aactatcagg
     7681 gatcaaaaca aagcgaatca cgttctgggt attcgtcaac atgggagtca tggcagcttt
     7741 agctggatta gttttagctg ctcgattgaa tgcggcaaca cctcaagcag gtacatcgat
     7801 ggaactggat gcaatggcgg cagtatattt tggtggtgct tcgacttccg gcggcattgg
     7861 aacaatcatg ggtgcgattg tcggtggctt ggtcatgggt gtattgaaca atggcatgtc
     7921 gattttaggc gtcggggtag actggcagca ggcaatcaaa ggattgatct tgcttttagc
     7981 cgttgtgcta gacttgtata ataagaaaaa acgaacaacg taggagaacg ccggttattc
     8041 agaaaaaaga ctgtgacaag acat
//
LOCUS       DACDSY010000128         7993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDSY010000128 DACDSY010000000
VERSION     DACDSY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.130"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            82..582
                     /locus_tag="IWK44_002328"
     CDS             82..582
                     /locus_tag="IWK44_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9550817.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(772..1116)
                     /locus_tag="IWK44_002329"
     CDS             complement(772..1116)
                     /locus_tag="IWK44_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP9550818.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(1270..2190)
                     /locus_tag="IWK44_002330"
     CDS             complement(1270..2190)
                     /locus_tag="IWK44_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9550819.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            2377..2868
                     /locus_tag="IWK44_002331"
     CDS             2377..2868
                     /locus_tag="IWK44_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP9550820.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(2892..5267)
                     /locus_tag="IWK44_002332"
     CDS             complement(2892..5267)
                     /locus_tag="IWK44_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP9550821.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            5593..6219
                     /locus_tag="IWK44_002333"
     CDS             5593..6219
                     /locus_tag="IWK44_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9550822.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            6242..6946
                     /locus_tag="IWK44_002334"
     CDS             6242..6946
                     /locus_tag="IWK44_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9550823.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7138..7971
                     /locus_tag="IWK44_002335"
     CDS             7138..7971
                     /locus_tag="IWK44_002335"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP9550824.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2455 a         1521 c         1447 g         2570 t
ORIGIN      
        1 ataaaaatag gcatggctat ttacaacaat agtgctatag tagaaaacaa atgaataagg
       61 ttacttaaag aggtgaatac gatgaaactg atcaaactat gggaagcaga tttaaagaaa
      121 gcttatcagc tacaaactag ctttggacca aatgagagtg gatttatcaa caatgcttat
      181 ggctatacat ttgctgaatt tttagattat gtaaaaaaat gcgaaaaaac gtctttagga
      241 atcgatcttc ctgagagtta cgttcccgca accgtcttta ttttagaaag tgatgaacat
      301 gagtatgtag gaatttttaa tttgcgccat aaactgaacg aaacattaag aaacggcgct
      361 ggccatattg gttacggcat ctcccctaat tatcgtaaaa aaggatatgc cacacaagga
      421 ttagcattag tactacaaga ggcaaaaaaa atcggcattg atgaggcact tttgtctgta
      481 aataaaaaaa atcttggcag tcttaaagca caacaaaaaa atggggctgc gatttatcgt
      541 gaagatgaga aagaatacta tacgaaaatc aaactgatgt aaaaaagaag gattccaaaa
      601 ggaaagggat ttgaatgtcc tttatcaata gaacacattt atttaattaa cttataatat
      661 agcagacaaa attttataaa caaagaaacc ttttatctct tttcttctag aaaggataaa
      721 aggctttttt gtttttccta tttgcttctc atagctagtc agccaagatt tctatcgtat
      781 ccccaacgca atccgtgcat aacggctcat cttatctgtc gtccatgctg gataccagac
      841 tagtttgact tctgctttcg ttacttctgg cacttctttt aatgcgtcat ggatagattc
      901 tgttaaaata tctgctaatg gacagcccat agttgttaga gtcatcttaa tgactgtttc
      961 tccattttca ggattgaatt ctatatcata aatcaagcct aaattgacga tgtcgattcc
     1021 taattcagga tcaatcacca tttccaaggc agcaagaata cgttctttga ttagttctat
     1081 ttcttgtcca gaccgttctt ggtttgcttc gctcatcgta cgacactcct taacacgatt
     1141 tttatttatc atactctttt tttagctgct tgtaaatgag aatttagaag taagattctc
     1201 gtttaaattt ataaaaatgt tcttattaga tgtaatagat taaatttatt ctaaaataaa
     1261 cctgattcct catttttttt gtgaacgatt ttttatttct ggcaatacgc cagttgttcg
     1321 atacattttt tgttcgtttt catttgcaaa ccggccaaat actgaatgag ttggtaaaat
     1381 attgataaat gcatcgtcgt cgatttcatg aacgatctga tcaagatcat acatttcgta
     1441 gcgggtgatg accatcatga tcatgttccc ttgttttttt gaatagccac cgacagacgg
     1501 cagtaatgtc attccacgta ccatccgttt tgctagttct tctgttacct cttctggctt
     1561 gacggtaacg atcattgctg ttactttttg atgactcgtg tggatggcat caattacttg
     1621 agtcattgca taaatggaaa taatcgtata caaggcactt tcccagttaa agaagaagcc
     1681 tgcgatgact acaatgatac catttagaat cagcatgtag tttccgactg tttttccagt
     1741 tgtttgtgaa agtacaagag agatgatatc catcccacca gttgtaaagc ccatttttaa
     1801 agataatcct acgccaagac ctaataaaac accgcctact agggcattca tcaaaatatt
     1861 atcagtgatc tgtccgactg gaagcagcat cgtcatcacc gatacacata caacattcac
     1921 aaaactaagg atcgttgctc gttttcctac tttgagaaac cctaatacaa acacagggat
     1981 attcaaaaga aaaataaatg ttcctgtgtc tatcttgata tgaaaccatt catataaaag
     2041 cgaggaaatg atttgagcga tcccattcat ccctgcagaa aagacattgg ctggaatcaa
     2101 aaaataattt aaagcgaccg cagcggtcaa tccggttatc aaaataacca ctattttctt
     2161 taatctttca tttttatgat acgcagtcat aattttactc acaagcacct ctcccctact
     2221 ttggtattcc aaatgtaaga ataacagttt atctattaaa ataaaatact tcgaactcaa
     2281 atgagaagaa ttttagcaaa caggtaagaa tctgccctaa tcctatgata agatagatgt
     2341 atggtaaata tttatatgta tatcaggagg gttcctatgg caaaagatgc tagcttcgac
     2401 attgtatcag aagtcaatac agaagaagta aaaaatgcga ttcagcaaac aacaaaagaa
     2461 ttgtccaatc gctttgactt caaggattct acagtagaaa tcaaattaga gaacagcaat
     2521 ctagttgtat tgagcgatga tgatttcaaa atcgaacaga tcaaagatgt attatttggt
     2581 aagctgatca aaagaaatgt gccaatcaaa aatatccatt tttccgaatc agaacacgct
     2641 ctaggtggca aagctcgaca aactgccgat ttggtcaacg gaatcgatcg tgaaaacgcg
     2701 aagaaaatca ctacagctat caaaaatgaa aaactgaaag taaaagcaca aatccaagaa
     2761 gatcagatta gagtgacagg taagaaccgt gatgatctac aagctgtgat tgctttatta
     2821 cgtaaacttg acttaccaat cgagttgcaa ttcactaact atcgttaatc aaaaaacgag
     2881 gctgacgtct gtcattcctc gtttttttga tttactgctt cttttgataa ccaaaaacgc
     2941 tgtattcctt cttccaattc cccaacactt cgtacttctc ttgctgtttg ccaatgttgt
     3001 gggaataaac gtcgataaga aaaactagaa aatcgttgat ctgccaatac aacgattcct
     3061 tgatccgcca tgccacgaat cactcgacca ccagcttgca agactttgtt cattccagga
     3121 agctgataag cgtaagcaaa tccaaggtgt tccttctcat cataatatga tttgatcagt
     3181 tcctgctcat gattcatctg cggcaaacct acacctacga tcatactgcc gatcaatcgg
     3241 tcccctctca aatcgattcc ttctgaaaaa atcccgccaa gtacgcaaaa tccaatcaat
     3301 gtttcagcgg gatttgtttg aaattttgtt aaaaatcgtt cgcgttcttc ctcattcatg
     3361 tccgtttctt ggattagcac attgctgtcg ggatattgct gtctgaatgc ttccaccgca
     3421 agatctaaat attgataaga aggaaagaaa acgaaataat tcccgacctt tccatcagca
     3481 aaagcatgaa tcgtctctac taattttgcc agactgtgtt ccctttttcg ataagtcgtc
     3541 tctaggtaat ttgcgatgaa aacttgctgg tttccttctg ggaaaggact cggcagtttg
     3601 taagccagac ttttttgtcc ccccagtgtt tcctgataat aagagagtgg tgaaaaacta
     3661 gcagaaaaaa gaatactgct ttttccttta tccaacgact gctcaagaaa caagcttgga
     3721 tcaatacaaa actcttttac tatcagatcg cgatacgttt tttcgactgt tgtttcgtag
     3781 tgatcatcat aaaattcact gaccttcaaa aaatgaagca aatcaaaata ataaggaagc
     3841 aattgttctt gtgatgggtg ctcaggaaat tctgccagcc attctttaat tttttcgctt
     3901 aattgataac ctgcctttac taaagattca gccggtgctt tttgatgatg ataatcccaa
     3961 tgatcttctt ttgctatttc tcggatcgaa tcaaattctt ttaatagttt gttgaaacgg
     4021 cgatgaagtt ttttgaattc ctttggtata gcttccttac ttcgtttcgt tttttcataa
     4081 gatagttctg ccgaatacat ctctcgggaa cgattcacca aattatgtgc ttcgtcaatc
     4141 aaaaacagat agtcctcgtt tttttcatct tcaaaaaaac gtcttaaata aacagttgga
     4201 tcaaataaat aattgtaatc cccaatgatt acgtcacacc agatactgac atctaatgat
     4261 agttcgaaag gacaaagtgt atgctttctt gcataagttt cgatgacttc acgagtgatc
     4321 tgattctcat gattcaaaag atcccataaa ccttcattaa tccgattgta ataaccattg
     4381 gcatatgggc actgatctgg attacaggtc gtctcatcta aaaagcagat cttgtctttc
     4441 gccgtcaaag tgacactttt tacttcagct cctgtatctt ttaatgctgt catagcatct
     4501 tcagctactt gtctagttat tgttttagcc gttagataaa agatcttttc tccctcttct
     4561 tcccctacag ctttcaaagc ggggaataac gtagagattg tttttcctgt tccggtaggt
     4621 gcttctacaa acaatttctg tttcgtacgg atcgttttat aagcagctac cgctagttca
     4681 cgctgacctt tcctatactc atcatggggg aatttcaaag ccatcaaaga ggcattgcgt
     4741 acagtgcgcc agttttcttg aaaaacaagc cattctttat aatcttcagt cagcttttta
     4801 aaaaaagttt ctaactgctc tcttgtctga tgttcaacct ttctcgtgat cacttcttct
     4861 gccgtctgga agtaagtcaa ttgcagattt atttcttgta aatcatgttc ttgactatag
     4921 atataggcat acacccttgc ttgatgaaag aaaagttcca cttgttctgg ttctaaatct
     4981 tcaaatcgtg gttcggaggt tttgatttca tctatataat aaataccgtc tcttttgaaa
     5041 ataccgtctg cacggccttc aacgatcagg taatattcat ctaattgaac tgttgttttt
     5101 aaaaagactt ctttttcata gttttctccc gcatcttttt ggagcttgcg gtggattctc
     5161 gctccttcta atgcagtatg gttacttttt ttacgattgt cgatacttcc acgtcgcaaa
     5221 ataaattcta ctaagtgccg cacagatatt tttgactgtt tgatcatttc caccacctct
     5281 ttctctctaa gattacctga aaaaacctag tttagcaaaa agagctctct ttccaaacat
     5341 aaaaaaacac cctgctgatt aggcagggat ataggatata gaatttggat ttttggatga
     5401 atctaatgta acaaggacat attaaaatgc tatgaaaact tactttcctt ctctttagaa
     5461 tatccaaata cttttcatat agtctgataa ttcagataag taaagagaaa taagtacctg
     5521 tttttgtttc ctagcaatga atgcgttata ctaatggatg aaagaagttt tggctatcaa
     5581 ggaggaatgc acatgcatat cgaaagatta aaaacaggaa tgattgaaga aaactgttat
     5641 ttggtctaca acgaagaagc acttttgatc attgaccctg gagaagatgc ggaacgaatc
     5701 aaacaagaaa tcaaaaaaac aaaccaacag ccggtagcga ttttgcttac ccatactcac
     5761 tacgatcata tcggtgcagt ggaaccttta cgcgcgtatt atcaaatccc agtttatgtc
     5821 agcccactag agcaaaaatg gctaggtgat ccgattttga atctttctgg actcggtaga
     5881 catgatgata ttgctgatgt tattgtctcg cctgcagaat atgaatttga aatgaaaact
     5941 tatactttag gaaacatgtc ttttcgcgtc gtccctaccc cagggcattc tataggaagc
     6001 gtcagcttta tttttgatga ttttgtcgtt tctggcgacg ccctgtttag aggaagtatc
     6061 ggtagaaccg atctgtatac agggaattta gaacaattac ttcatagtat tcggacccag
     6121 ctgtttgtat tacctgatga atttgcagtc tatccaggac atggcgatgc aacgacgatt
     6181 gaacaggaaa aaaggacaaa tccttttttc aatcgttaaa acgacttagg aggagaataa
     6241 aatgacagaa actacacttt atatcgtacg acacggaaaa acgatgttca atacaattga
     6301 acgagtacaa ggctggtgtg atacacccct cacaaagcaa ggacaagaag gcattcatta
     6361 tttaggtcgc gggttaaaaa atgtcgactt ttcatttgct tattctagtg acagtggtcg
     6421 tgcaatcgaa actgcccgta ttattctagg agaacattct aagggaaaag aaattcctta
     6481 ttttattgaa aaaaggatta gagaatggtg tttcgggtct cttgaaggtg gatatgacat
     6541 ggaaatgtgg ggtgtgatcc ctcgtgtatt gaattttaaa acatatgatg aaatgtttac
     6601 cacagaagtt tcttttgaac aaattgctaa tgctgtatac aacttagata ccgctgggtg
     6661 ggcggaaact tatgaaacat tgcgagaccg tgtatggaca ggatttgaag atatcgccca
     6721 tcactgcgaa aaaaatggtg gtggaaatgt tttagtcgta tctcatggtt tgacgatcgc
     6781 ctttttgctt aatctgatcg atcctaaaca gcctgttcgt gctgggcttt taaatggcag
     6841 cgtaacaaag gtcacttatg caaacggtcg attttctatc cacggcatca atgatacgtc
     6901 ttatatcgat aacgggaaaa cacatagtcg cgactcgttt ctataatggg gatattttaa
     6961 tttttaccag agaatcataa aaggcaaagt ctgaacgagg gctttgtctt cttttttctc
     7021 ttcgtgtact tatctgcttt atttctcaag tttttcttaa tattaaaaaa cagattgggt
     7081 ttctcaaaaa ataacggtat actataagat gtatcttaat tgggaaggaa ggaatttatg
     7141 ttttttgcaa atattatcaa agcagtgatt ctcggtatta ttgaaggaat aacggaatgg
     7201 ctgcccatca gtagtactgg tcatttgatc ctagcagatg aattcatcaa actggatgcc
     7261 agttcacaat ttatgtcgat gttcaatgtc gttatccaat taggggcgat cttggctgtc
     7321 gtcgttcttt attttcacaa gttaaaccca ttttctccaa gtaaatcaac ggtagaaaaa
     7381 aaagatacgt ggacactttg gtttaaagtg atcgttgctt gcataccagc catcatcatt
     7441 ggtttgcctt tggatgattg gttagaagca aatttccaca agttcttacc agttgccatt
     7501 gttttgatcg tttacggtat tgcctttatt attgtcgaaa aacgcaacaa aaacaaaaca
     7561 cctcgttgga cttctctgaa tgactttacg taccaagcag ctcttcttgt tgggttattc
     7621 caagtcttgg cacttgttcc cggaacatcc cgttctggcg caacgatcct aggatctatc
     7681 ttgattggtg cttcgcgttt tgtcgctaca gagttttcat ttttcttagg gattcctgtc
     7741 atgtttggcg caagcggctt gaagatcgtc aaatatttag ctgaagggaa ttcattcaaa
     7801 atggaagaaa ctgttgttct tcttgtagga acattcgtat cttttgtggt ttccgtctta
     7861 gctatcaaat tcttactaag ctatttgaag aaaaacgact ttaccgcttt tggctggtat
     7921 cgtattatcc ttggtatcat tttgattggt tattggttta ttgcaatgta gaaaaaacca
     7981 gaacacttct ctt
//
LOCUS       DACDSY010000129         7895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDSY010000129 DACDSY010000000
VERSION     DACDSY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.131"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..136
                     /locus_tag="IWK44_002336"
     CDS             <1..136
                     /locus_tag="IWK44_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9550825.1"
                     /translation="YIQITPVLLGDGIPLFQKGTYEARFEMLDTTRFGEFIELHYVRK
                     "
     gene            complement(215..769)
                     /locus_tag="IWK44_002337"
     CDS             complement(215..769)
                     /locus_tag="IWK44_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9550826.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            1022..1297
                     /gene="rpsP"
                     /locus_tag="IWK44_002338"
     CDS             1022..1297
                     /gene="rpsP"
                     /locus_tag="IWK44_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP9550827.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            1309..1560
                     /locus_tag="IWK44_002339"
     CDS             1309..1560
                     /locus_tag="IWK44_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9550828.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            1685..2476
                     /locus_tag="IWK44_002340"
     CDS             1685..2476
                     /locus_tag="IWK44_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550829.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            2646..3167
                     /gene="rimM"
                     /locus_tag="IWK44_002341"
     CDS             2646..3167
                     /gene="rimM"
                     /locus_tag="IWK44_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP9550830.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            3157..3918
                     /gene="trmD"
                     /locus_tag="IWK44_002342"
     CDS             3157..3918
                     /gene="trmD"
                     /locus_tag="IWK44_002342"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP9550831.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            4054..4401
                     /gene="rplS"
                     /locus_tag="IWK44_002343"
     CDS             4054..4401
                     /gene="rplS"
                     /locus_tag="IWK44_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP9550832.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(4533..5669)
                     /locus_tag="IWK44_002344"
     CDS             complement(4533..5669)
                     /locus_tag="IWK44_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9550833.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(5785..6699)
                     /locus_tag="IWK44_002345"
     CDS             complement(5785..6699)
                     /locus_tag="IWK44_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP9550834.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(6790..7218)
                     /locus_tag="IWK44_002346"
     CDS             complement(6790..7218)
                     /locus_tag="IWK44_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9550835.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(7223..7597)
                     /locus_tag="IWK44_002347"
     CDS             complement(7223..7597)
                     /locus_tag="IWK44_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9550836.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
BASE COUNT         2524 a         1389 c         1417 g         2565 t
ORIGIN      
        1 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
       61 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
      121 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
      181 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
      241 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
      301 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
      361 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
      421 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
      481 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
      541 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
      601 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
      661 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
      721 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
      781 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
      841 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
      901 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
      961 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
     1021 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
     1081 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
     1141 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
     1201 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
     1261 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
     1321 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
     1381 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
     1441 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
     1501 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
     1561 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
     1621 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
     1681 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
     1741 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
     1801 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
     1861 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
     1921 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
     1981 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
     2041 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
     2101 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
     2161 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
     2221 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
     2281 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
     2341 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
     2401 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
     2461 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
     2521 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
     2581 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
     2641 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
     2701 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
     2761 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
     2821 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
     2881 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
     2941 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
     3001 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
     3061 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
     3121 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
     3181 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
     3241 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
     3301 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
     3361 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
     3421 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
     3481 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
     3541 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
     3601 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
     3661 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
     3721 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
     3781 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
     3841 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
     3901 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
     3961 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
     4021 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
     4081 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
     4141 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
     4201 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
     4261 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
     4321 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
     4381 atcaaagaaa tccgtcgtta atcatcgtta tcagactgag aaagaaccct tggtattcaa
     4441 gggttctttt tttgttctct gtttttttat attaacgatt tctatttggg acatttttga
     4501 ttaataaaaa ggggcaaaaa aggggcaaca agttacagtt tttctatatc ttctttcaat
     4561 ttttctttta ctccctttgt cacatgcaaa taaatctttt cagtgatatc actattttcg
     4621 tgcccaacgc gatcctgaat agcatacaga ggagttccta gttccgctaa ttttgaaata
     4681 tgggtatgtc taaaaatatg tgaactaagt tttttatcaa tcttcatgtc agctttatga
     4741 tttctcaaat aggtgttaat tgctgttagt tggaaaggag ttcctttcgt tgtttgaaat
     4801 agaaattgac cattcggatt taattctaga agctcattgt aaatagctat cgcttttttt
     4861 ggtaaatcaa tttctcgcat tccagcagca gtttttgtag aatcagattt tttcatatca
     4921 gctattgaac gttctctata catcatcgtc ccatttataa ctactgatgc attattattg
     4981 gtaatgtgta catcgtcttt acttaatgcg attgcttctc caggcctcat tccagtcaaa
     5041 tatagccact ggaaaaggag agaatatctt ttattgtgtg aagttgtgaa atttactagt
     5101 cttttatatt catcatcttc taaaaactta tctttaattt ttatcgtttt tgactctcgt
     5161 ttataatcaa taactacttc gttgatagga tttttttcta catagccttt tttcatagcg
     5221 tatgaaaaaa gaagattcaa tttggattta attacactga cgtacttatt tgacaagtca
     5281 tctttatata tcatatcttc aaaaatatta tttaaatcta ctgtattaat accagaaacg
     5341 atgtaagtct caggtatctt tttctttata gtgtttaaga tattattcgt aggataatat
     5401 gtagactcct taacttgtct tttgtaaata actaaccatt cttcgacaag ctcatgaaat
     5461 gttagatcag gcttctgaag tgttttttcg tttaatttta tatcaatttt tttatttaac
     5521 tctagcaatg ccactttctg agtttctcga gatttatttt tataagtaat gctcactttt
     5581 tttctttttc tagtttttgg atcaatatat cgctcattat atttatatac tttttttccg
     5641 tttttatcag ttttggtttc aatccacatt gttttcatct cctatttgtt gctataatag
     5701 gaatggataa gtaagccaat tatagcaggt ttatttttca tcacgtccac aaactttggc
     5761 gagggagggg gcgtgttttt ttgtttagta gctacctttc catgaagtat ctgcgaagaa
     5821 cacttcaaat gttgttccat catttttcac ttcaaaatat gctgttcctt gtgcggattt
     5881 acccggttgg atggtttccg aaaagtaatc tctactagct aaatcagatt taatatctga
     5941 tgaatcataa aattctaaat aacttgaact tgcatcaaaa ggtgtagtcc ctgtgttttc
     6001 tacaagaaaa tctacctttg cgtaaaatag gccttctggt ttatagaaat catcaccgta
     6061 atcttttgat acagactgga cagttacgtt aatagagtta ccattttcag aagtaaaagg
     6121 aactgcttct cctatactat gtgcaactga tgccgtctgg ggttctgaag acgaagttgt
     6181 agttgtagtt gtagtagagc ttgaatcttt ggattttcca tctttatcta agaaagttcg
     6241 ttgattataa tatacaacct gttcagtttt tggactaacc caaattaaca ttgtttcatc
     6301 atcagaatca gttgaataag tatattggag catctctaat ttttttatat cttttgcact
     6361 atttccatgt tcaattaatt cagagtgctc tttataataa tctccttgtg catcctttga
     6421 aatagataca attaattcat cccgtgtgat agcattgtct aattctttgt aaacaaaatc
     6481 atttgtccat ttacgtaacg gtgaaccaag tgtctgataa acttgagtta aatcatcacc
     6541 aacttttatt ttatgaacgt ctgatgaagt tagttttttt gtttcatatt cagatgttga
     6601 tgaaacagta ttttctttag tgctagcaga attaggacta gtggagcatc cgcttaatac
     6661 caagcctaaa aaaagcaacc caaaaacaat ttttttcatt tttaattcct cacttctatg
     6721 atatgatttt tttgtaggat cttagaaata aggtttttag tccgtgttgc agcacggact
     6781 ttttttacat tatataagaa agcgataagc gctttctgga agtccgtaaa gattctttaa
     6841 ttcctcaatt cgtttaggat attgatcatt gtcttctttg tatagagaaa caatgagatt
     6901 agcagcaaag caattagctt cgctttcaga tttgcttcta gatgttcttg ttgatacata
     6961 gtaactggat aagccacgat gaaaaatagc gtgacctaat tcgtgagcgc aaatgtagaa
     7021 tctttcctca gagtctctca gttcatcatt taagaagatt attgcacgac ctctaatttc
     7081 ttgaaactgt cctttgggat tctcgataaa aggaacgtat tgaattttaa tgcccatctt
     7141 ttcacaaata taaaaaggat tagcggactg gtatttccgc ttcaactcct cgactaaatt
     7201 aatcgtatcc atctccataa gctcacatct ttttgccttt ttctttgtct tctttcacaa
     7261 tatcccagaa ggttccaata aggatatctt ttacgcgttg gacctgttca ggcgtcagtg
     7321 tttctccgcc ataagacata ttgacatttg aatctagtaa tttatcaagt tcaactactt
     7381 catcttttgt tgcccagtca ggaacttggt ttctaccgag taaataatca gtagttacat
     7441 taaaataatc tgctacaagt tgtagtcttt tagtactagg agttctattt ttccattgat
     7501 aaatcgtgtt tttaggtata tttaattctt cttccaattg agttatactt atgcctcttt
     7561 tatgagctaa ttcctttatt ctatctagta aattcatttt cgcacctcac aagctacgaa
     7621 aacactaata aaaaaattag ttaaaggtgt tgacaactaa taaaagtgtt agtatactgt
     7681 tttcgtaagc taaaatatta gctaaaaaga cttgataact aataaacact tcacggtcgg
     7741 caaactctga aatgtaaatt actaggcatt tctgtgtctt atttagctat gtctatatac
     7801 taataaaaat attagttatt gtcaacgaat attagctaat tttttagctt acagattatt
     7861 tttttagaaa ggagctattt ttatgtctga gaatt
//
LOCUS       DACDSY010000130         7693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDSY010000130 DACDSY010000000
VERSION     DACDSY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.132"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            18..2345
                     /locus_tag="IWK44_002348"
     CDS             18..2345
                     /locus_tag="IWK44_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9550837.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2471..2800
                     /locus_tag="IWK44_002349"
     CDS             2471..2800
                     /locus_tag="IWK44_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9550838.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2840..3844
                     /locus_tag="IWK44_002350"
     CDS             2840..3844
                     /locus_tag="IWK44_002350"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9550839.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3841..4287
                     /locus_tag="IWK44_002351"
     CDS             3841..4287
                     /locus_tag="IWK44_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9550840.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4437..5252
                     /locus_tag="IWK44_002352"
     CDS             4437..5252
                     /locus_tag="IWK44_002352"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9550841.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5458..6630
                     /locus_tag="IWK44_002353"
     CDS             5458..6630
                     /locus_tag="IWK44_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9550842.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6792..7286)
                     /locus_tag="IWK44_002354"
     CDS             complement(6792..7286)
                     /locus_tag="IWK44_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550843.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
BASE COUNT         2590 a         1453 c         1530 g         2120 t
ORIGIN      
        1 ttttattgag aggagggatg aaaatgatta agaaaactca aaataaaatc caacgtatat
       61 gtcaaaaaca ttttgtttct agactcaaag ctgtatctaa aagaaaatac ttacatccca
      121 ttatgatttc gatcatcttg atttttactt tcttagggag cttatcaagt atttatgtaa
      181 acgtttatac agaaacgtca aattttgcta accaaacgac agaggagaca aatagtccac
      241 cctattctga aatacagtcc atagaaagca ccaatcagtc tagcaacaac gagacaacct
      301 caaccacatc ctcgactcaa ctgattgatc cgacagaaaa agaaatcaca gcagcaaaag
      361 cagcagggga gaaagcttat tcagaaacag gtagaagcca gacatttagt actgttgcgg
      421 ccactgcgaa tttcaacgga cataccatac cagatggata tacagtgata tatgccaaag
      481 atggagtcat tgttgcggtt aacggatcga tgagtgcgga taaaactgcg ttacaaggcg
      541 taaccaaagc aatcgctttt attgataacc gtccagatat ccataatctg tttattggct
      601 ccacagtaaa tggtactact atcacattcc caaaaagtat ctatatgggg gtggctaccg
      661 ctggaaatac gatcgattca gttacaccca atgcggataa aagttattct tttacaccct
      721 caacatgggg agggcttcct gccggaggag gtatgagtac tttttcaatc ggtatacagt
      781 tagcaaatga tattccattc acggggtact cgatcaaaaa tgggcagatc gttgatggta
      841 agggaaatgt ttcaaatttt ccaacctatc aagcagcaac ggttcaggtc aatgcgaatc
      901 ggacaggaac tttttcttat acagtcaatg gtggagcagc aaaaatcgga acgtacggga
      961 ctacattatc tggaatccat gtgggtgaca aagtggttat cactccaaca gcagtaacgg
     1021 gctattcttt tgcacaaaca aacggttctt tcacagccct agaaagtaac gtcgatacgg
     1081 tgacctatac aggcaaacaa acaccaatca ctattaagta tcaagactat tttggacaga
     1141 tgattgctcc ttctaaaata atgaatctta cttacggaag tgctgcacaa gacctgacga
     1201 ctaatacgcc tactatcaac ggatatacct ttacagctgt ttcagcgata gaaacaaaga
     1261 accaatcagc aatgagtgtt ttagctagtt tagatacaaa tggaaatgta atcgtaaagg
     1321 acgcaaatgg aaaacagatt tctgaagtca tcctatatta taagacaaat gcaacagtat
     1381 caatcaatgc aaatggcagt aagtactatg atggtctttc tgttgtaccg atcgttaaat
     1441 atgcctttaa tgataaaaca gaatcaacat cccttttaaa caatgatctc agtattgatc
     1501 aaatcacatg gaatccaact gatttcaaag cgatgaatga aaagggagtt gaagtaactg
     1561 cgcctactga aattggaacc tatacgtctt ggcaattgac aaaaagcgga ctagctaaac
     1621 tagctgccag aacaaactat ttatttacta tcgtgcagac atctgatgtc tatacgatta
     1681 agcaaatcag cggtgcggta gagctcagtg gcagtaagac atatgatgga aaagcaggtg
     1741 tcccgtcaat ccatgtcaag ctagcagagg gtgtcactag ttcgcttact ccagtacaag
     1801 tggcacttag cagtatggat tacacagcag atgcacaatc agcaaaaaat atggttaatg
     1861 ctggcagcta tacaattaaa ctaacgaata gcggaataga caaagtcaaa gcagcaaatc
     1921 ccaagttgtc atttactgat atagccaaca catcaggaac ttataccatc tataaagcta
     1981 acgcagtgat tactgtcgat gacgctgcct ttaattatga tgcccaaagt cactcgattt
     2041 cggttggaaa tctccacgtt accggtgttg tcttaggcga atcattggat tataccctaa
     2101 cagttaacag ccggacagat attggatcac aaatagtaac gattagttta ggcgaaggaa
     2161 gtgtaaacaa taattatacg attatcgcaa cagataccgc taagttgacg atcaatccga
     2221 ctctcttgct tccttcgaca ggtgggatag gtctactgcc ctttgtcttt ttaggactga
     2281 tctttatttt ttctggcttc ttttatttta tccatcgtaa gaaaaaagca ggtgaacagc
     2341 gatgaaaaaa acaaaaaatg tacgtgtgga tatatttgct gttctctttt ttatcctact
     2401 ggtctttttt ataggttctc cgactgtgtc agctagagaa tcacctatta catttacttt
     2461 gcataatcga atgttcgaaa gcgataagat gccaagtatc agtcaaaacg acggaataaa
     2521 tatcccggat aactttcaag gagtaaaagg gttgaacggc gttactttcg ctatatatga
     2581 tgtctcggat gaattttaca agctgcggtc agaaggaagt agtgtggaag acgcacaaag
     2641 aaaactagca caaaaaagtg atagtgaaaa aatactggca gaaaatgtga caaaaacagt
     2701 agacgaggaa gagggcattg cttcttttag tgcatcggac aaagatgagc aaggtcgtga
     2761 cgctgtttac cgatttacgg aaataaagac ttcagattaa gtaaaagaaa aatcagcacc
     2821 atttgtcgtt gtgcttcctg tgacagattc atctaatcat aaattgacga ccatccactt
     2881 gtatccaaag agcgaacaaa aaatacctgc tgcactcaca ttaacaaaaa cagtagagaa
     2941 taagcagaca gattttgcag atggggataa agtcccttat ctcatcaccg taacaattcc
     3001 tgagaacatc aacgagatag gaacctatac gatcaaagat acagctgatc ctcagttatg
     3061 gctagaacca gaaacgatcg attttttgat tggtggagac aagattcata catttcacac
     3121 acaaaaaata aaacatggtt ttgtcttatc agattctgga aaagatctta gttcgtttgc
     3181 tggaaaaaaa ttaacgatcc gttaccaaat gacattgaaa gaaaacagtg aaaaaacgac
     3241 atttgataac gatgtacgtt tgacgactga caatcaaaca gtagaaacac atcttgccat
     3301 ccaaacagga ggcaaacaat ttgtcaaagt ggatcggcaa acgaaacaaa aactagcaga
     3361 tgctcaattt ctagtcaaaa aaaatgacca atacttagcg caagaaaacg ggataaatca
     3421 ttgggtagcc aaagatgatt caagagcaac gatttttact tccagtaaag atggaacatt
     3481 ttctgtccat ggattgtctt ttggaagtta cgaactagtg gagattaaac cgcctaaagg
     3541 atatatattg agtcaatcaa ccattctctt tgaagttgaa aaaggcagtt atgatccgct
     3601 tcatcctata ccattggaaa tcatcaacga accaaaagca aatccgacga ataatggaaa
     3661 aacgccagtt cagatgcaga caaatggtcc atcaaatgaa aaaaatggag gaagctttcc
     3721 aaaaacaagc gaagagatgc ttggaggttt ctcaatttta gggttattgc tagtcctttc
     3781 aacagggaca gcttggtttt ataaaaaaca acaaaaagga aacaatagga gggaaataaa
     3841 atgaaaaaca tcaaaacaaa aatatggacg tttttaggaa ctgctatcat gctgcttcct
     3901 ttcgttctcg gcctaggaac tgcagaggtt tctgcagccg tatcacctac accagaaaat
     3961 gttacggtta atttgcataa attgaaattc acttcagcac cagaaaatca aataaacaac
     4021 ggaacggaat tgacatttcc gaatagcgaa ccgttgaacg gcgttgaatt caacgtatat
     4081 gacatcactg ctacgtacta tccaagtaaa gacacagcag ttccagcaga cgctacaccc
     4141 ttcgcaagtg taacgacttc tggtgaagga ttagctaacc tgactcttcc aggtaaatct
     4201 gatgggaaag atgctgtata cgtctttgta gaaacaccaa agcccggtgt cgaaacgtcg
     4261 ccaaatatcg tgttaagctt accctagacc ctgacaaacc tttagacacg attcacctat
     4321 atgctaaaaa tgtcatcaaa gaaagcaata tcaaaatcca gaaagctggg aacttttcag
     4381 gggtagataa gttagcaggg gcgcaattcc agatcaaaaa ttcagagaat caattcgtga
     4441 ccggttttga tgaaaacact ggtgtcgctc tctatgatcc tgctgagtca gcgttgacat
     4501 tcacaactga tactgaagga tccatcagtg tcaatggatt aatagacggc aattatactt
     4561 tagttgaaac aaaagcacca gatggttatc aattaaccaa tccacaaaaa gaaactactt
     4621 ttatagtaaa aaacggaaaa ttgcaacaaa caaccggtat catccatggc gcagatgaag
     4681 ccaatcttgg gaatccaaac ggtgtagtca atcgggaaaa tacaactacg gataacacga
     4741 ttactgtaga aaatgtccaa aactacggtg atttccactt catcaaacaa gatgtgaaca
     4801 cgcaagaaaa attagccgat gcagaatttt ctattctaag agcaaaagat ccaactgtta
     4861 aattaatgaa aaaaatagca aaccttgcct caggagaata cccatatgct tggtcagatg
     4921 aagtcacagg ttctgactgg gaagaagtga aattgatttc tgattcagaa gggaaatttt
     4981 caatcgaagg gctgaaacaa ggggcatact gccttcaaga aacaaaagca ccagaaggct
     5041 acgcattacc taagaatgaa gctgcgtaca ctgaatttac agtagatgat gatccaacaa
     5101 cttctgatga aacaacgatc gataaccaac ctaaaggaat cttgccttca actggtggta
     5161 tggggatcat cgtattcatc ttggtaggta ctgctttagt tggaggagca gtgatctatt
     5221 tcaaaaaacg ccatcaagaa acagaagctt aaactattgg aaacaaaaat catacgataa
     5281 ataaacgata gtgattcaaa tggggagaaa ggttgtgaca agtggtcggt ctcttggtat
     5341 tcgctcaaca aacggcaaaa taggaggaac tattttgccg tttgcttcgc taatactcag
     5401 gtcaaggcgc tgatttcaca acctcttcat atacatatta gaaaataggt ggacatgatg
     5461 aataaaagca gaataagaat actggacatc ttcatggcaa tcattttagt cgttgggatc
     5521 gggatctttt cttatccttt tgtagaagat tcactgaatg attttcttgc ccagcaaatg
     5581 atcatccatt atcaaaagca agcatcgaat aagaattcag aggaaataaa aaaacagcag
     5641 gaaaaaatga caaagaaaaa ccaacagttg gcagagcaaa acgtatcacc agggataggc
     5701 agtttcaacc aagcagttga tgcaaaagca ttaaaagatt tacctagtaa tgcctttttt
     5761 atggaacata tgcttggagt catcgaaatc ccaaaaatca atgtcagctt acctatattc
     5821 gatcagacaa cggaaatatt tttgcaaaaa ggaacgtcat tattggaagg aagttcttat
     5881 ccgactggcg ggaaaaacac ccatgccgtt ctttctgggc atcgtggact cccagaagca
     5941 aaattattta cagatttgcc taagttgaaa aaaggagacc agtttttcat ccagatcaat
     6001 ggaaagacat tggcgtatca agtagaaaaa atccaagtgg tcttacctga cgaagtagat
     6061 tctttgggta tccaaaaagg cagagatctc gtgactttgc ttacctgcac tccttatatg
     6121 gtcaatacac atcgactgct tgtgacgggg catcgtattc cttatcaagc aaaagaggca
     6181 aaaaaagcga tccaaggaat tgatcaatgg aaaaaatgga aattttttat ttggtttatc
     6241 ggtatactac ttggatcaat cggcttagtt tggctactca tcgcttattt agattctttg
     6301 gccatcgcta agcgaaacta tcctttatct ttttatgtaa agaacacaaa cggacgacca
     6361 atcgaaggaa tggtcttttc tgtaaaaacg ctcaacggca aacactatat tactagagaa
     6421 aaagttccat ttgtgaaagc ttctgatgaa tacggactag tgagattttc tgatttaaaa
     6481 ggcaggaact atcgcttgca acatgaggag ctcctgctta aaatccatgt aaaacataaa
     6541 cattcgaaac agttttcaat gaaattgaaa aaaggaagat ataaactgcg aaaagaaaaa
     6601 gaggtgtact atttgattga gaaagaatga atgaagatgt ccaaaaaagc agagggaaca
     6661 gactaaatga aaaaagagag aagctgacga aatggacgtg taaactgcta tttttggttt
     6721 actatctgga acgccagctt ctattttttt atgtttcaga agaattttat tattatagta
     6781 taacttttga attagtttcc ataacatttg gagcaagggg ttaaaccacg agcttgagct
     6841 tcgcttaaag tggcaggggt atacgtacca tttccacatt tatgtgtatg gtattttgaa
     6901 ccggtcggtg tgacatagac agtttgttca ttattttgct caggagctgc ggcttgtgcc
     6961 tgctgagaag cctgttcagc tgctgcttgc tgtcgcgcct gttctgcttg ttgctgtgcg
     7021 attgcttctt gcctcgcctg ttctgcttgt tgctgtgcga ttgcttcttg ttgcgcttgt
     7081 tctgcttgtt gtcgtgcaat ttcttcttgc tttttccgtt cttcttctgc ttcttttgct
     7141 ttgattgtgg aatcaattgc agcaagacgg cttgtcaact cttgtttgtt ttcaggtagc
     7201 gcttggatag ctgctgaagc agttgcatag ttttcttttg taggatttgc ttcagcttgt
     7261 gacaaagcag attccgcttc tgtcactttt ttttctagtg cagctttttc cgctttttgt
     7321 ttctcttctt cttttttctt ttcaatttgt tctttcgatg aatctgccgt actttcggag
     7381 atcgtggtac tgctgctgga agcctgagct gaacttgtgc tttgagtagt agacgtagta
     7441 gcaccacacc cactcaacaa acttcctgcg accaaaaaca acgcaactaa ttttttcact
     7501 aaatttcctc cttaagtaaa aaacaaccgt tttcaataaa tgtccttttc aattgtttac
     7561 ttacttgtta taataattgt gttatgaata gtgattgagg aaacttaatc taatcccgtg
     7621 ttgatacacg ggattttttg ctatagttct gttattaagt atgcaataat aaaatatgta
     7681 cgttatttta tta
//
LOCUS       DACDSY010000131         7639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDSY010000131 DACDSY010000000
VERSION     DACDSY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.133"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(60..788)
                     /locus_tag="IWK44_002355"
     CDS             complement(60..788)
                     /locus_tag="IWK44_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9550844.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(801..2081)
                     /locus_tag="IWK44_002356"
     CDS             complement(801..2081)
                     /locus_tag="IWK44_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9550845.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2222..2824)
                     /locus_tag="IWK44_002357"
     CDS             complement(2222..2824)
                     /locus_tag="IWK44_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP9550846.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3069..3275
                     /locus_tag="IWK44_002358"
     CDS             3069..3275
                     /locus_tag="IWK44_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550847.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3587..4306
                     /locus_tag="IWK44_002359"
     CDS             3587..4306
                     /locus_tag="IWK44_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9550848.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4387..5070)
                     /locus_tag="IWK44_002360"
     CDS             complement(4387..5070)
                     /locus_tag="IWK44_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP9550849.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5288..7474
                     /locus_tag="IWK44_002361"
     CDS             5288..7474
                     /locus_tag="IWK44_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9550850.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2735 a         1288 c         1439 g         2177 t
ORIGIN      
        1 aagaaccgaa gggtttggat cggctggtca tttagtcggt ccaaactatt cggttctttt
       61 tatgaaatca atttctttaa tccacggata gaggctggga tggtctcgcc gtttgccaac
      121 gtgacttctc tggttttttt attaatcgac cgcacatttt gtgtattgat aacataggat
      181 ttatgacaac ggataaaatt ccgatgcatt ttctcgattt ctttgatctt ctcatagaat
      241 tgtaattggg catgagtcaa atggacttcc agtttatgtg gcgctcctag cgaagtagaa
      301 aaatattgga tgtctttgat cggtaaaaat ttgatctcat cattaaactc tattttgaag
      361 atatcttttt ggatttggga catttgaaaa tcttcgaaaa ttttgctcag gatatgctcg
      421 acattttctt ttaaatcata aagatcttct ttgacgatat aatcagtcgg ttcgatgttg
      481 ctttttaaga ttttcatcga catttccgta tgggaagtca catagattat ttttcctaaa
      541 ggatctaatt cacggatcaa tttaccgatc gctacaccat ccatttcact gtcttctagt
      601 tcgatatcta aaaagtaaat accaaaagaa gaagttctga tcttggctgc attgagaagt
      661 tcttccggat cagaggtaga aatatggaca taactgtcta agttttcaat catgattcta
      721 tttttgatca ccgttgataa aacttctaat tgttttggat cgtcttcaca aatataaatg
      781 ggaatcatat cattactcct ttattttatt tgtatataaa tcgtttgaga aaaacgctgg
      841 ttttcaattg atgtttcaag gaaaagataa ggattggtct ttgtcaattc atccaagata
      901 aacaagccaa ggccttcgtt ttttggattt gtttttgttg agaacccttt ttgtttcaac
      961 tggtttaacg gtaatgtatt tgttgttgta ttgatcacac gaatcatcac ggaatgattc
     1021 ttcttgagta tggtcaattc gatttttttc ttctttgcag cactagctcc ttcaatcgca
     1081 ttgtccaaga taataccaag catacgtaca aggtttaccg tatgtttcgt atccagttcg
     1141 attggctcag gaaaatgaag ctggacgatc aacccttttt cctgtgccat catgagtttt
     1201 aatgacagta aactacggat ctcaggaact ttcatattat ccaacatact tagattggcc
     1261 ggaaaaaggt tcatcatctg gcgtgtaggt aatatcgttt gatcataata agaccgtaat
     1321 ccttctaagt cgccttctga aatatagctg tgaagagaaa aaagcagatt ttggtaatca
     1381 tgtttgaatt ctctcaagtc ttgatagttt ttttctagat ctttgatata atcctgttgc
     1441 atcctgactt gttgctctaa taactgatca gctaatttct tattagagat tttccaataa
     1501 taaaccaaga tgatcaggcc aacacacaga tatagaatga aaaaactcgt attcaaagct
     1561 aagacatcag gtgtttcacc agaaaaacgg gtataaaaaa taatgatata gtatgtaaat
     1621 aatatcaatg tcccgatcgt accgatcata cggcggtcca cagtgtgaaa ccaagtctct
     1681 aacgattttt ttaaacaaaa agctaataaa gcagataaaa aagcggataa gacaatgtga
     1741 aaatactgca atgaatctcc tggttcaata accgaagatt tcaaaacaaa aaaatcgtcg
     1801 atgcttgtta agtgatctcc taaaagcgac gtcaataacc caaaaggaag cgcgtaaacc
     1861 aattgttgac gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc tatagcaatt
     1921 agagcagtaa aaatccctaa aatcgaatag acccatccaa tcaaaataca agaaatcaga
     1981 atgactcccg caaacagata tttgtatcgg atgtgatggt caatcaataa gatacaaatt
     2041 gtgtaaatca aatattgcaa cgctatggac cataaaaaca taaagtacag ctcccattga
     2101 tttttaccaa attatagcac aattagcgta ttattttttt aatttttcag ggattttgaa
     2161 ttcagaactc ataccaaagc agcagccaga tacaaaaaag tcagataagt tagttaattg
     2221 tttaatgatt gtttttttca tgaaagattc ctccaaaatt catcaagtag tttgtaatag
     2281 gaagcaacgt agctatttct aaaacaatcc caagtagcaa ataggtagaa aagtaagaag
     2341 ctgagaaaaa taaagtaatg agagaaaaaa ttagaaacat gctgatcgcc ttctttaata
     2401 atgggggacg tttctcgggt tcaatgggat tgattgctgt acctcttgat ccatagcgaa
     2461 gcagtaacaa aaagcagctg attaacaaaa agaccatcat tgggaaggag atagtcaatt
     2521 gcagatgttt catcaccaag ggaacgcccc ataaataaag tagtgtccac agcagacaaa
     2581 cgaattcaga gtcggaatgc aatccataag catatctgcg gatgcagata taagaaagaa
     2641 aggtgattcc tgtttccgcc catgagcctg tcaccagaga aaagactaga acgaccgcta
     2701 gttttccaag attattcaat aaaacttcta aggcaaattt tacttggaca tacaccatat
     2761 cttcttccga tttgtctccg ctgaataagg cttctaataa tttgttcaca actttatctt
     2821 tcatctcata cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa acgtcttttg
     2881 tttcataaaa agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa atcttcgttt
     2941 tccaagaacc ttaccaaaaa aagtgacagt tttgatacga aaagtacttt ttatgacaag
     3001 tttgtgcttt ttcgagtgat ttatgttaaa aaattactga ccttataaaa ggtgatggag
     3061 ggattcttat ggaaaatgaa caattgtttg aagtcggcga aacatatgca tttaaatctg
     3121 atttatttga atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat agtgtgatga
     3181 tacgtgtcat gaaatgcgca gtagaagaca atgataccgc tcataatctg cactggcagt
     3241 tagttgtacg caaatcaagt acaatagaag aataaaaaca gataaaatgt tgagagcata
     3301 aagaggttgt gagtctcccg tttagctctg agttacaagg aataatgtta aaaaacagct
     3361 tttcatgttt tatgaatatt gccttgtgac cgaggtgttg gcaggagaat actgtttatt
     3421 tgaaaaaagg gctgcgacaa gacttttgtc acagccttta ttttcatgaa taggagttac
     3481 ttttggctac ttttagagaa gtagctttta aggagcagat aaagtcaagg cttttatttc
     3541 tataaatgaa gtagtaaaat gaagaacaga ttttattaca ggaaggatgc cgataattgt
     3601 gatcattgtt gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa tgcttgccca
     3661 agatttagaa acaaaacctt tttatgagaa cgtggaagat aatgaagtat tgccgctatt
     3721 ttattctaat ccagaaaagt atactttttt attacagatt ttctttttga acaaacgctt
     3781 tttggcaatc aaagatgctt tcagccatga tgacaacgta ctagaccgct ccatctacga
     3841 agatagtatg ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag tagaagtaaa
     3901 gcagtatgaa ggtcttttag aaacgatgct gaaggagtta gaagaaattt cgccacaaaa
     3961 gaaaccagat ctactggttt atattcgtgt atcctttgaa actatgttag cgagaatcaa
     4021 aaaaagaggc cgggaatatg aacagctaga acaagatcct gaactctatt cctactataa
     4081 agaactcaat cgacgatacg aagagtggta tgaacagttt gatatatgtc ccaaaatagt
     4141 gatcgatggg gacaaatatg atttcgtagc cgatccggca tgtggagaac aaatcgtaca
     4201 agaaatcaag atgcgggcaa agaaaatgac agaagaggca gatgcccagt cgatccgcta
     4261 cactacaaat aacccgaaag caaagggaat ctttacaaat aaataatata aaaactaagc
     4321 aaattgctca ctcgcagata tttctctgtg agcagcatct tgcttagttt ttttgattca
     4381 ttgagatcaa atagtgaacc gattcgtatg ataaaattgc cataaatgaa atgtctgagt
     4441 gagggccatg atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc caaacagatg
     4501 atgacagaaa acaaggacaa tagctcctgc gataaaggca agaacgacac ttagatagtg
     4561 gaaaaatcga tcaaaatctg ctttttcttt agaaatacaa taatcatata agttcgttgc
     4621 tacccctttt aaatttccag ttgtaaataa attagtgtag gcacgcccgt tgattttatc
     4681 aaaagctacc cactggatcg atgcagtaaa agcaatcaaa atcgttacta atgcgggatg
     4741 ctcatggacg ggtgtcaaac taacaaagaa aaaaatcact gcttcataat atagaacaaa
     4801 caagcgccag aaatgttttt ctttttttct aaaataatca attagatact ttgttagaag
     4861 aatccctaag aaaaagaaaa gggtagacaa tactttttta ccaacactta gccattctcc
     4921 gtcaaatgct tggatgatcg atagaatgat attgccagtt tgagcagagg caaaagcacc
     4981 atattggata taagtataac tgtccatggc tcctccgata aatgtcagca aaagtcctat
     5041 tgtacgcccc tcgtgaaagg aagtctgcat ttttttcatc accttttatg aatatttttg
     5101 tcttccttat cttagcataa acgattgaaa aaaaggaaaa attttcagaa aactaaaatc
     5161 atatcgcttc ccattttcat ttttagaaag gtctgttagt ataaagaaaa aatagacaac
     5221 gaaactttaa ataaccaact tgaaataaac aaattcaaag aagaaagatg ggaaggaaat
     5281 caatcagttg aatgaaaaag aaattctccg attacaaaaa gaattgaaac gctgggagaa
     5341 caagtataaa aagcttcaac aagtctttcg gctacaagaa gaaatcattg ctacctatga
     5401 agcggcttat ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa aagaaagtga
     5461 aggaaacagc tctttcttta aaatgatgga gaaacgttcc tataatcgct tcaatcaaga
     5521 acaaaaagca gctatcacat atgatatgaa gaaacatctt cgaatcatag caggagctgg
     5581 gagcggcaaa acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc aaaaacaagt
     5641 agacgaagaa cgcatactaa tgatcacttt tacaaggaac gctgcaaatg aactaaaaaa
     5701 acgagtagat aatttcagcc agagaaaaac cgctgtccat atcggaacat tccatagtat
     5761 cttttttagg atatacaatg aaatttgtcg aaaatttcct gaagtagcaa tgcaaggaat
     5821 acaaggagac ttttcaaagg atactgcaca aaaagtgaat gctgttttgc aacagctgat
     5881 acgtaaatat aatctttata tatttgatca atatggagaa aaaacgattg cttctcggtt
     5941 agactattgg cagaatatga acctttcagt agaagaaatg gttcagttag ttaaagaaaa
     6001 atatgattcc attgataaga atgcacgaca gccgatatca gaacgactgt atggattgct
     6061 cacggaactc caagaacaaa aaagaagcca gcaattgcta gagttcaatg atgtactcca
     6121 aaatctgaaa agcgctttgg agaatgagga gatccagcat tatatcggcc aaaaatatga
     6181 ttatctattc attgatgaat ttcaagatac taatccactt caatgggaaa tcgtgaaacg
     6241 gatgacaaag gaaaacagga tcaagttgat tgttgttgga gatgatgatc agagtatcta
     6301 tggattcaga ggatcagagc cagcatacat caagaacttt gaaaaagaat atcctaccaa
     6361 aactcttttc ttattgacta attatcgttc tcgggcagct attgtccaag gagcaaatag
     6421 attgatcagc tataataaaa atgatcggat accaaaggcg atgattccag cacaaaaaga
     6481 agcaggtgtt atggaagcct atttgttttc tgatactaaa gcagaaagtc agtggctaat
     6541 caaacaaatt cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa tcatattata
     6601 ccgatcagct tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga atattccttt
     6661 tgtactggaa gcagatagtc catatgaagg tatctttggt atcaagtctt tccaacattt
     6721 ctatcaaaaa attgttcatt ggcaaacagc actgaatctg caaaagaaaa atcaggccta
     6781 ccagcaattg ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg aatgcgatca
     6841 atacttttct cctaaatatc aaaatatagc cgttgcagat tatattttgt cgataagccc
     6901 caatttaaaa tcgaaatctg cagaaatccg agagtttgag gaagcgttgc gtaaaacaga
     6961 ccagcatcaa gaaatctatt cgcttgttca aagctatctt catctgccac gtatagctaa
     7021 ggaaatcgac cagagtgatc aagaatggat acgagaagaa gtaaagcagc atgctacttt
     7081 cagaagactc caagccttat cgcaagaaac ccaagcgaca gctaaagaat taaaaacccg
     7141 cttgattgct tacagacaag gaaaacttga tgcactatgt atccagagca ttcacaaaag
     7201 taaaggtctt agttaccgaa atgtcttttt gattggttgt aacgaaggaa gtttgcctta
     7261 taacggagcg acagaaaaga aagtaatcga ctttaatgaa ataaaagcag aaccagtgac
     7321 gacgatagaa gaagaacgaa gactatttta tgttgcgatg actagagcaa gaaatcgtct
     7381 gtatctatgt gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga gatttatcaa
     7441 agaaaccggg agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg acagaagtgg
     7501 tcagtttcaa gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt tgtgaaattt
     7561 cagcttattt tcgaagaagc tatttctgga acacagttta ttcggaaaag ggaggtgtga
     7621 caagaatttt gtcacacct
//
LOCUS       DACDSY010000132         7544 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDSY010000132 DACDSY010000000
VERSION     DACDSY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.134"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            13..492
                     /locus_tag="IWK44_002362"
     CDS             13..492
                     /locus_tag="IWK44_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9550851.1"
                     /translation="MVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITNDLKLSTSMIHL
                     IDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNKKTKLVSLTKY
                     GRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEYQVSLTRKED"
     gene            585..1747
                     /locus_tag="IWK44_002363"
     CDS             join(585..861,861..1747)
                     /locus_tag="IWK44_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9550852.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2027..3007
                     /locus_tag="IWK44_002364"
     CDS             2027..3007
                     /locus_tag="IWK44_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9550853.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3129..4547
                     /locus_tag="IWK44_002365"
     CDS             3129..4547
                     /locus_tag="IWK44_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP9550854.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4618..6087
                     /locus_tag="IWK44_002366"
     CDS             4618..6087
                     /locus_tag="IWK44_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9550855.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6097..6564
                     /locus_tag="IWK44_002367"
     CDS             6097..6564
                     /locus_tag="IWK44_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9550856.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6600..7472
                     /locus_tag="IWK44_002368"
     CDS             6600..7472
                     /locus_tag="IWK44_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9550857.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
BASE COUNT         2579 a         1148 c         1557 g         2260 t
ORIGIN      
        1 agaggtgttt atgtggttga taaaattcag tttaatcaag ttatagaaga attcatcaaa
       61 gtaaataatt taatgaccca tattcaaaaa cgaccaatca gaataactaa tgatctaaaa
      121 ctcagtacga gtatgattca tttgattgat gtgattggaa attatcctga aactagtatt
      181 acggaattag ctgatcgatt aggcgttacc aaaggagctg tatcccaaca gattcccact
      241 ttagagaaaa tgaatcttat aagcatttct caaaaaaaag gaaataagaa aactaaactt
      301 gtatcgttaa caaaatatgg tcgaaaggtg tacgaatcac ataattattt acatgaagag
      361 ctttatagct caataaatga agcgttagaa tcattctcac cagaccaaat ttcaaccatt
      421 aaacagattc tagaaaaagt atcttataca atcaatgaat atcaagtaag tctgacaaga
      481 aaggaagatt gacatgaaaa aaatgatttt tgaatcggct acactagact agacagaaaa
      541 aataaggtgt gtaaaataaa tgaaaacaca ctggaggaat ttttatgtca agacgtcaac
      601 gtagaaccta ttcaaaagaa ttcaaacaac aagtcgtcga tctttatctc gcaggaaagc
      661 cccgagctga aatcattcga gattatgagc tgactacctc ggcctttgat aagtgggtca
      721 agcaagcgca aactactggc tcgtttcagg aaaaagataa cttgatccct gaacaagtag
      781 aactaattgc attacgaaag aaaaataaac agcttgaaat ggagaatgac attttaaagc
      841 aagcggcgct gatattggga cgaaaagaca agtaattgat accaataagc acaaatactc
      901 aatatcagcg atgtgtaaag tgttaaacat ctcttgccaa acctattatt atcaagccaa
      961 accagttgaa gatgaatcag aactggaaga actcgttcaa gaagaattta ttcgcaatcg
     1021 aaaagcttat ggtaccagga agctgaagca gtgcttggcc aatctaggtc ttcaagtgag
     1081 tcaacgcaga atcggccgta tgatgaaaca gcgtggcttg cgttcaacat acacattggc
     1141 tcatttcaaa ttccattctt ctacttgtaa cgaagccaag accgccaata ttctagacgg
     1201 aaccttccta caggaaaagt cattagaagc gattgtaacc gaccttacct atgttcgtgt
     1261 tgggaagaaa tggcagtata tctgcctgat tcttgatttg tttaatcgag aaatcatcgg
     1321 ctattcatgc ggggagaaga aagatgcgac gttagtcaag gaagcttttg caaagattcc
     1381 gtactcgttg aataatgtac aactttttca cacggatcgt ggaaaagaat tcgataacca
     1441 gacgattgac gaaattttat gggcctttaa tatcacgcgt tccttaagca aaaaaggctg
     1501 cccctatgat aacgccgttg cagaatccac ctataaatct gtcaaagtag aattcgttca
     1561 tcaataccgc tttgagacat tggctaattt gcgattagag ctatttgatt acgtccattg
     1621 gtggaatacc atccggctgc acggcacgtt aggctatgag acaccgatca gttttcgaca
     1681 gcagagattg gcgcagcgaa tccttgataa tgagctcgga tgtgataccg gtggcgaggc
     1741 accttaatta tttgaatgag cttctgcctt agagaatcag attcgaggag gctcgttgtc
     1801 aagggcagtc ggaacaccag cagcaagctt acgcaatacc ttataatttt tgtcaaaaaa
     1861 agtgttgcca ttccacatat atctttaaac agctattgcc agataaaaat aaatgatatt
     1921 atatatataa atacttatcc gaaaagggga atgagagagt caaggttctt ggaatgtcga
     1981 atagtcgagt aggtagattt tctatctaca accttgttgt ttaaaaatga aaaaaattgg
     2041 aattagagaa gttgcgaaag aggcaaatgt ttctcctacg actgtgtcga gggtattgaa
     2101 tgatcgtgga tatttgagtg aggaaacaaa aagaaaagtt tttgatgcca tgaaaaaatt
     2161 agaatactat cctaatgaac tggcgagagc actttttaaa aacgaaacat ataccgtagg
     2221 tttgattttt cctacaatta ctaatccatt tcacgctgag ttaattcaag atatcgagtt
     2281 cgagctttcc agcaggggat ataaagtgtt gctgtgcaat agcttaaaca atccaacaaa
     2341 agaaaaagag tacttgacga tgttgagaaa gaatcaggtt gatggaatta tagtaggaac
     2401 gcacaataaa gatattgcgg attatgatat tccagggcta cctattgtag cgattgatag
     2461 aagtcttggt gaaaatacag ttacagtatc ttgtgataat tacaggggtg gggaattagc
     2521 tactcaacta cttgtaaatc atggctgtca gaatatactg tgtattcggg gagatagtaa
     2581 gataaagcta cccgcgaata gtaggacttt agcatataaa gatgttttga ataaattcat
     2641 gctggaaccg ttagtcctag aagtaccatt tgtaaaagaa atcaatgaaa aaaatgaaat
     2701 aattaagaca tatctggaaa ctcatccaaa tattgacggg gtttttgcag gagatgattt
     2761 attggcttct ttggcgatta acaacttagg aagttcgggc aaaagagttc caaaagaggt
     2821 taaagtgatt ggatttgatg gagctcaaca aacattgatt tataatccag aattatcaac
     2881 tattcaacag cctatagatc aaatatctaa agtagcagta aaaaaattat tgaatatgat
     2941 taagggcgaa aaggagacgg atgaactttc acttccagta aagctcataa aaaaaaatac
     3001 agcataagtt aaatcggcga aaaattcctt cgtcgattta aaaatatttt tatgaaacgg
     3061 gttgacatat gaaacgggtt gacatatatt agttgtgaag ataaaaacct tttcactgga
     3121 ggaagaaaat ggagaaatta ttaattaaaa gcaataatca tcaactttac taccaaccta
     3181 aggatgtttg ggtcggagat gttatgcctt acgggaaaga aggaaaattc tttatttatc
     3241 atcaacgtga tactagaaat ccagttcctt ttggagaacc gtttggctgg gctttagcaa
     3301 ctacgaaaga ttttgttcat tttgaagact ttggggaatc tcttttaaga ggggatgaca
     3361 atgagagaga ccagtttatc tacgctggtt ctgtatttga ggcaaatgga aaggttcacg
     3421 cattttatac agggtataat cgtgaattct tagcgcaggg aaagacatct caagtgttgc
     3481 ttcatgctga gagtgatgat tttgttcatt gggaaaaatc agaaacagca ttggagttag
     3541 aaccacaaga agggtatgat attcgaaact ggagagatcc atttgtcgtt tgggatgatg
     3601 aaaaagaaga atatttgcta attttagggg cgcgaaaggg tcaggataaa catctacaaa
     3661 ccggacgttt agtggaattt acatcaaaag atttaactaa ttgggaattc aaaggtgatt
     3721 tttgggctcc gggattgtat accatgtttg aaatgccaga acttttcaag attggtgact
     3781 ggtggtatct tgtctattca gaatactcag atggaaataa aatactctat cgaatgagtc
     3841 aaagtttgga aggtccttgg attaagccta aagatgaagc ttttgatgga cgggcttatt
     3901 atgcaggtag aacagcttat gatggaaaac gtcgagtcct atttggatgg gtaccaacaa
     3961 aagatcaaga aaatgataaa aacaattatt tatggggagg tactttggtt cctcatgaag
     4021 tctatcaaaa agaggatttt tcacttgggg taaaacccat agacgaagtg tggaatgcat
     4081 tcataaattt acatgatatt gccgatgtag agatagaaac agcagacaca agagaagaag
     4141 taacgcttgt caaaaatagt ggaactttgt ttaatttaga aacaacaatt tcatttgatg
     4201 agaatcttag tgacttttca ctgagattat ataaaaacct tgagacagat gaatcttacg
     4261 aatatcgctt caatttagat gaggggcgtc ttacatttga taaaaaccct tgttaccctt
     4321 ggtttcaata tatggataaa ggacttatta gacctatatc tttaaagcct aaccaatctt
     4381 ataaactaaa agtgattgtt gatgacaata taatgacact ttatattgat ggaatcgctc
     4441 ttaacgctcg tgtacatggt tgttttggtc aagcaattac tattacagca acaaatggat
     4501 caatgactgc aataaatacc aagttttcaa atgaaataaa gaaataataa acgtcaatca
     4561 tgtggcaggt ctgaaaatca aacatgacct gtcacattta aaagggagga taaatgaatg
     4621 gactacaaaa aaattgccga agatgtattg gaaaacgttg gaggaaaaga gaatatctca
     4681 agcgctacac actgcattac tcgtttacgt ttaattctta aagacaaaag caaagcgaat
     4741 atggaaatgc ttgaggaaat agaaggtgca aaaggtgttc ttttcaatag tggacagtta
     4801 caagtaattt tcggaacagg tataattgaa aaggtttaca aagagttcgt tgaattgaca
     4861 ggagctaagg aagtatccgt ttctgaaatg aaagacgaag gagtcaataa ttcaggaaat
     4921 agacttcaac gagcttttaa agttttctcg gatatcttta ttccgatcat tccagctttt
     4981 attggggcag caatggtttt aggaattcgt tcgttgatta ccactcctgg aatgtttgga
     5041 atggatggct cattcgctga tcacaatgta ttcctaaatg atttatccaa atttctgggt
     5101 attattgcaa ccacatttga ttatttacca gttttagtta tgtattctgc aacaaaacgt
     5161 tttggcggaa accctatttt aggattactt gtaggatttg ttatgattca tccaggacta
     5221 gccaatcgga atgatttcgt catgggaact ataaagcctg attattggca tttatttgga
     5281 ttaagcattc cagcagttgc gtttcaaggg ggtgtttttc cagcaatttt gacttcttgg
     5341 tttttagcaa aagttgagaa aattacaaat aaatattctc ctcaagtact tgcttacatt
     5401 ttagtcccaa ctgtgacaat tgttcttgcc aactttgcac tatttttagt gtttggtcca
     5461 ttggggaatt ttatcggtaa tgttttaggt ggaatcattg atttcctata tatgaagatg
     5521 ggtgcctttg gtgcttttgt ttttgcaggc gcgttacaac cattagttgt aacaggaaca
     5581 caacatgcta ttcaaggtat tgaggcaaac ttagtcgcaa caactggatt caattatatc
     5641 caacctattt ggtcagtttc aattattgcc caaggtggcg gttgtattgg tatgtattta
     5701 ctctttaaga aaaaatctaa agatagagat attgcgattt caagctttat tccaacactg
     5761 gtaggtatta ctgaaccggc tatctttgct gttaatttga aatattcaat cgttcctttt
     5821 gtttgctcgt ttattggtgc tggttttggt ggagcattta tgaaaatttt tgatgtaaaa
     5881 gccattggac aaggattgac tgtattgcca ggtctgacta ttgctaatcc agttcttcct
     5941 tatatatttg gtaatctcat agcattcatt ttaccaatca tttttattct cgcttatgat
     6001 aaatataaag tgtttatccc tgaagctaaa caccgtaaat ttgcagaaaa aactgactca
     6061 aaaaaattgc cagttcaagt aaagtgagat gagagcatgt taaatttttt tagaaaaaat
     6121 gaaataagcg ttgtagcacc tgcaaccggt gtttttgaaa aactggagaa attaagtgat
     6181 ccagtctttt caaaaggaat gatgggacaa ggatttgcaa ttgatgcaaa aaacgaaacg
     6241 attgtatctc caatcgatgg ttttgtttca agtatttttc ctacaaaaca tgccttagga
     6301 ataaaaacaa aatcaggaat tgagatactt gttcatgtag gaatagatac agttgagcta
     6361 aatggtgagg gatttgatat taaagttcaa gaaggacaaa aagttaaggc tggggatatt
     6421 cttatgacca ttaattttgt agtttttgaa aagaatcaca agactaaaga tgtcattgtt
     6481 gtttttccag actaccaaga cgaaattttc attccgaaac ttgaagaagt aaaacaagga
     6541 caagaattgt ttgttcttgg ctaatgataa gtaattaata atagaatcga ggaaaatgta
     6601 tggtttttgg ggctattgaa gctggtggaa caaaatttgt ttgtgccatt ggagataaag
     6661 atttgaacat atttgaaaaa gtcagctttc ccacgactac acccaaagaa acaatgaatt
     6721 tagttttaga tttttttaat catcataaag atgatttaga aggtatcgga gtgggatcgt
     6781 tcggtcctat tgatattcat tgtgaatcta aaacgtatgg cttcattaca tcaacgccaa
     6841 aacttgcttg gcaggatttt gattttattg gaagtttaaa aaagcaactt aatattccaa
     6901 ctgcttggac gactgatgta aatgcctctt gctatggtga atatgtttct ggaaatggca
     6961 gaggcctttc aagtgttgtt tactataccg taggaacggg cataggaggt ggcgctatcc
     7021 aaaacggaaa cttcattgaa ggttttagtc atccagaaat gggacatatg cttgtaaaac
     7081 gacacaataa agacaacttt tctgggacat gtccattcca cggagactgt ttagagggct
     7141 tagctgctgg tccggcaatc gaaagaagat cagggaaaaa gggacaagaa gtgactgaaa
     7201 atgacgttac ttgggaaata gaagcttatt acattgctca gtgtgcatat aatacaacat
     7261 tgatgttatc acctgatgtc attatatttg gtggcggagt aatgaaacaa aaacacttaa
     7321 aaaagaaagt acaagataag tttattgagt tgatcaacgg atatgtcgct attcccgatg
     7381 ttgacaatta tattatgaca ccaaaattgt tagataattc tggtattgta ggttgtttag
     7441 ctttagcaag agacgttaaa gaaaaatctt aatttggaaa aaagatcgtc tcgactattt
     7501 gtttggttga tgcgatcttt ttttcaatta gtaattgaag ggac
//
LOCUS       DACDSY010000133         7480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDSY010000133 DACDSY010000000
VERSION     DACDSY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.135"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(161..838)
                     /gene="nth"
                     /locus_tag="IWK44_002369"
     CDS             complement(161..838)
                     /gene="nth"
                     /locus_tag="IWK44_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP9550858.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(845..1543)
                     /locus_tag="IWK44_002370"
     CDS             complement(845..1543)
                     /locus_tag="IWK44_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP9550859.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1811..2995
                     /locus_tag="IWK44_002371"
     CDS             1811..2995
                     /locus_tag="IWK44_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP9550860.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3001..3444)
                     /locus_tag="IWK44_002372"
     CDS             complement(3001..3444)
                     /locus_tag="IWK44_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9550861.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3670..3957)
                     /locus_tag="IWK44_002373"
     CDS             complement(3670..3957)
                     /locus_tag="IWK44_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550862.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4128..5480)
                     /locus_tag="IWK44_002374"
     CDS             complement(4128..5480)
                     /locus_tag="IWK44_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP9550863.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5753..6199)
                     /locus_tag="IWK44_002375"
     CDS             complement(5753..6199)
                     /locus_tag="IWK44_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9550864.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6435..7361)
                     /gene="cysK"
                     /locus_tag="IWK44_002376"
     CDS             complement(6435..7361)
                     /gene="cysK"
                     /locus_tag="IWK44_002376"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP9550865.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2017 a         1536 c         1229 g         2698 t
ORIGIN      
        1 ataacaactt ttatttttat cgcaataaat tgtaataagt taccatccta gctactgagt
       61 atgtacattt actttttcat gcaaaaaaga taaacgaaac agcttttctc cataataaca
      121 agataattca tacatttatt tgcaattaaa gaaaccacgt ctacaggcgt ggtttctttt
      181 ttagcgcctt ttcttttaga cgcatccgat ttttcccatc ttgacatatt gatagcaaag
      241 gacatacctc gcacttggga tttcttgccg tgcaatgata tctaccaaaa aaaatcagtg
      301 tatgatgtgt ctttacccat aattcttgtg gtactttccg catcagtgtt tcttctactt
      361 ccatgaccgt agcatctagt ttgcagatcc tcaggcgttt tgagacacgt tccacgtggg
      421 tatctactgc gattgctggt atcccgaatg catcccccaa tacgacattt gctgtctttc
      481 tgcctacacc aggaagagac atcaattctt ctcttgacgt tggcacttgc ccatcgaaac
      541 gttcaatcag ctgctgtgca cacgctttga tgttttttgc tttatttcga tacaaaccaa
      601 tcgtttttat tttaagaatg atttcatcga ttgaagcttc tgctaacgca tcgggtgtcg
      661 gaaaagaggc aaacaaatct ggcgttgctt tattcactga tacatctgtc gcctgggcac
      721 ttaagatcac tgcaatcaat aactcaaatg gattattgtg cttgagttct ccatgtgctt
      781 cgggaaacat tccatacatc gtttctagag cttccattgt tttttgtttg cttaacatat
      841 tccgctactc ctccaaccaa tttttcagtg agactttcgg caattcttct tgttgagctg
      901 actgttctac ttctctttga agaagctgct gttttcttcg tttttggtct tcttccactt
      961 gttgttttgt acgtagattt tttctttccc acgatagcaa aatgcggtca acgtaattaa
     1021 aactataagc ctgatttaat acggcttccc gcagtgctag cttcaaaatt tccggctgat
     1081 aatggtcttc ttctagccat tgtccgatcc gctggaactc gatcgaagaa agcgggcgtc
     1141 caaattcttg ttcaaataat tgatacactg actggatttc tttttcttgt gttttttctt
     1201 cgtatttttt ttcttgtgtt tttaaatact cacctaatag atcatagatg gggtaaagat
     1261 tatatcgatc tgctttttta ccgttgacaa tcgtcgtttc tatcttgata aatccatttg
     1321 ttacgagacg atccagtatt tgaaaaatct gatcttgttt gattcccata tccatagcaa
     1381 tcatctgcaa atcagggaaa gtatctcctt caagctgtgc catatgcaac tgtagaacaa
     1441 ataaaaattc ttcagattgt agaccgatcc tatgataatt tttaagtaaa agattagata
     1501 tgactgtttg tccagcgctt aagtaatttt ctaaattcaa catgatttct cctccgctca
     1561 atcacagtat acgtaaaaaa aacatgggaa gcaagaacag gtttcttttc tatttacttc
     1621 actgatcggt cagatttaca atgtcgtcta cttgattttt gtttgttcat gtttttgatg
     1681 cgtatcttca cttttcggaa taaataaatt aagtagattt cgctgttcct tagaatgttg
     1741 acaaggaaac aaaattacta tacactattt ttgtttccaa gttaacatta ttgcataaag
     1801 gagtttttac atgtacagtt tcacaaataa acattctaaa gtttttggat acgtctctgt
     1861 cttattcact ggattaggat ttactatcgt cagtcctgta cttccgtttt taacgctgcc
     1921 ttattctcac tccatacatc agcaagcttt ctatatcact ttgctcatgt ctgtttatgc
     1981 tctagctgcc ttcttatctg cccccattct cggtagctta agtgatcatt ttgggcgtca
     2041 tccgatcctt attatcagtt tattaggatc aagtatcgga tatttgcttt ttggtttagg
     2101 aaattcgata tggatgttgt tccttggtcg tatcattgaa ggattgacgg caggagaaat
     2161 ctccacctta tatgcttact ttgccgacat cacggaacct aatgaaagaa ccaaagtttt
     2221 tggatggatg ggtgctttag tcggtatagg aacaacgctg ggtcctatta tagggggtct
     2281 attagctgag ttggggaatt ctgttcctat atttattgga gcactcttta ctttcttaaa
     2341 tgccgtttat ggctacacat ttatgctaga aagccttcca ataaaaaaac gttcagtaga
     2401 tttaagtttc tcccatgtaa gaccttttca ccaattacaa caattattca agatttcatc
     2461 cgttattcct ttattgacag caggttttgc tgtttggttg gcagctggct ctcttcaatc
     2521 tatcttttct caattttcga ttgacgcctt tcagtggaag gctgggttgg ttggtttaag
     2581 cttctctttg attggaatct tggatactgt ctcgcaattg ttcattatgc ctcgattgct
     2641 caaaaaattt tcagaacagc agatcacaag aatagggatg ttcagtgaaa tcctcgccta
     2701 tctattcatc acattatctg gaattcttct actgccgatt ttgtttttat ttgggattat
     2761 ctgttatggt ttcggtgatt ctatttttac tcctgtattc aatggccaac tatccaattc
     2821 tgtctcagat aaccagcaag gtcttgtaat gggcggtacc caaagtatac aatctctatc
     2881 gcgcgtcatc ggcccgctaa tcgcaggaca gctttatgct gcagcaccat ttcttccgac
     2941 ggtattcgga tttttgctta tgatttttgc ctattatcac tacaataaaa aatagtagct
     3001 tcatttgcta cttcgtataa attctctgta tcgaccgata aaacgaaata ctgtgtcaaa
     3061 ttcttctttt gacatatttt caaatacagt agcatctcgc tgtacccatt tttgatggag
     3121 ttgctcgtgt ctattcctta ttttttgtcc gattggcgtc aattgaaaat agatttcttt
     3181 tttattctct tcgctttgat aagtgtttat gatccctcta gctatcagtt tttttgttag
     3241 cttactgatg gctcctctag tcatgtaact agcattcgct aattttgtca cgttaggatc
     3301 agggattttc ccgatatgtt caatcacttc tagttcattt agagtaaatc ctgcaaattc
     3361 tttttccatt tctggtttat tacgatgaat aatctcatta gctaaatcca gatattcttc
     3421 tattagcagt tgtcttttat ccatttctac accttctttt tctttcatta tattgttttt
     3481 ttgtttccta ttcaacaaag ataaaaatat tttgtaggaa aacccaatct gaaaccaaga
     3541 aaaaaagggg ttgacaaagc ttgtcataac ccctttttct gattaaaaga tagtaaagag
     3601 acagtcatcg gtaattgtga tgattttttg tcatcactta aggatgagcg gccttttcaa
     3661 aaactgtctt taaagatcat catcagtttc tagcacatat ggctgacctt catatgtgta
     3721 ctctgtattc aatgtgccgt cattttcatc tgagtagatg cgtctactga aaggaaaacg
     3781 atgagaaaca ttatctttgg cgtcatttgc tggcatcgtg taatattcct cttgcgtttc
     3841 attcaactga gagagtactt gatctgccaa ctcatcaata ttgatcgtta atttcttcga
     3901 agcactttta taatcttcta aaaatgcatc ttgttggaca aaatattctt tattcatctt
     3961 ctttctcacc tttcttttca aaatcaaata aagctgcttg ttcttttatg taacagcttt
     4021 atctttgctg cttattgtga caaaaaattc gaagaaagac aactaattgt acttttcaca
     4081 aaaaaagaca gagggtcgtg acaaaattgt gtcacaaccc tttttcttta ttgtgcagtt
     4141 tcgatttcgc ctagcgctgc ttgtgcaagt aggttcaagt aattccatgg gcggtcaaaa
     4201 tgtggttgga agaagaaatc tgaaagtgcg agatcttcta cagtcatttt attttgaaca
     4261 gctaaagaca tggtatttgc tgattgtgta atgtcgtatt tggacatgaa ctgagcacct
     4321 acgatacgat tcgttccttt ttcatagact agctccatca ttactttttc agtagttggc
     4381 atgaactctg gacgataatt atcttcaaat acagtcgctt ggacttctag atcgttcaaa
     4441 gttgcgcttt ctttggtcac acctgtcgaa ccgatcgtcc agccaaatag gtaaagtcct
     4501 gaagttcctt gagtaccacg atacgccatt ttatgatctg tcaagttatt tcccacaagc
     4561 aaaccttgac gaacggcatt tgttgccaaa ggaatatagt tttgcgtgcc acttggattg
     4621 tagttgacta ctgcagagtc tccagctgca aaaatgtctg gattacttgt ctgcatgtat
     4681 tcattcactt tgattgcccc gtttggcagt gtatctactt ttccttcgat cagttttgta
     4741 tttggacgga agcccacaca taaaatgacc atatctgtat caaatgtatc tgttggtgta
     4801 gctacttgtt tcacttttcc ttcttcatct gtgataaatt cagtaacatt ttcgcctagc
     4861 gcaagagtaa caccgcggtc tgtcaattct ttttctaaga tatctgtgaa tggtttatct
     4921 agatatttat tcaagatgcg atctaatcca tcaatcaatg tcacttgttt tccagattcg
     4981 acaaaagctt ctactaattc aataccgata tatccaccac caacaattac tacacgtttt
     5041 gcttctttcg cttgtgcaat aatttcattt gcttgattgt agtttttgca taggagtaca
     5101 tttttcgcat cgattccact aatcggtggg ataataggcc atgaaccagt tgtcatcact
     5161 aatttatcat aagaaaccgt ttctgtttct cctgttttca gatcttttgc cgttactgtt
     5221 ttattttctg tatcgacgtc tgttacatcg tgttccattt taactgttgc tcctaatgaa
     5281 gataattctt caggatttga gtagaacaag cctgctgggt cctttactac tccgccgaca
     5341 taaagtgcga taccgcaaga taagaaagaa atattgtcat ttctttcata tactgtcact
     5401 tctgcttctg gatgattttt taaaatactt tttacagctg cagtacctgc atgagtacag
     5461 ccaattacta ctactttcat ttatggtccc tccgattaac attttgtcct tcgacaaaac
     5521 tactataaca gaaataaaac ggatacaaca gtttaatgct tgtgaattca caaaggtttg
     5581 tttgtagttt cattcctttt caagcataag aacccaatat ctacaagatt cttattttaa
     5641 taatattcac ttttatatct aagattgtga ataaaagaca aaaaaataaa aaagctagcg
     5701 ttttttttac acagctagct ttttttaggt ataacacttg tagaaactgt ttttacattt
     5761 gcgccttttt tgctgactct ttacaatccg gacagagtcc atacaattcc aaccgatgtt
     5821 cattaatctc aaaatcagtc agtctgcttg cagccatttc tatatcttca agccctgggt
     5881 aatgaaaatc aacaatctta ccgcagtttt cacaaattac atgataatga cgctttgaac
     5941 tgaaatcaaa tcggctagaa gcatcgccgt acttcatttc ttgaacaaat ccaatcgaag
     6001 taaataagcg aaggttattg taaacagtcg caacactcat atttggaaac cgatgttcaa
     6061 gtgctttata aatctcatca gccgttggat gggaatgatg ttcaatcaaa tattctaaaa
     6121 ccgcgtaccg ttgaggtgtg attcgaatgt tctctttttt taacttttcg ataacctctt
     6181 cgactaactt attttccatg taaacacccc acgttgttat tctcgtctta tcatactatt
     6241 tttacatttt aaaatccact taacatgaaa agaaaattca attttattgt attttaatga
     6301 aaaaagacgt aaaacagtcg cgttcactat tctacgtctc tattcaaata atttttctca
     6361 tcataaattt ttgtgtgttc ttttcatctt taaaagtttt ttacataaac ctatctggag
     6421 aaaaaccaag tgccttatat ctcttggtaa agggctgtag ataaataacg ttcaccattg
     6481 tcgggaacca cagctaatac cttcttgcct tttcctaatt cttttgcgac ttttagcgcg
     6541 gcagcaattg cagcgccaga tgagatgcct actaaaatcc cttcttttcg cccaacttct
     6601 ctagctgttt ccatcgcttc gtctccacta atagaaagga ctttatcata cacagaagta
     6661 tccagtgttt taggaacaaa acctgtaccg attccttgga ttttgtgagg tcccgcttct
     6721 ttcccttcta aaatagcaga ttctgctggt tctaccccga ttaattcgat tttcggatac
     6781 acacgtttta attctcggcc agcgccagtg atcgttcctc ctgtaccgat acctgcaaca
     6841 aacgcgtcca agccatttac tccaaatgct tgatgtatct caggaccagt cgtcttctca
     6901 tgcacaagag gatttgcttc attttcaaat tgtaatggta aaaagtagcc gttttctttt
     6961 gccaaacgtt ctgcttctct gattgatcca gaaataccat ccgcaccagg agtcaaaatc
     7021 aactctgcgc cgtacccttt catcaataaa cgtctttcga tactcatcgt ctcaggcatc
     7081 acgatgatta ctttatatcc ttttgcaact cctaccatcg acaatccgat tcctgtattt
     7141 ccagaagtag gttcaatgat agtatcacct ggtttcagta gaccatcgtg ttctgctttt
     7201 tctatcatac ttaaagcaat acgatctttt acacttcctc ctggattaaa aaactcaagc
     7261 tttacaaaaa catctgctga atcttctggt acgattttat ttaatttgac gattggcgtt
     7321 ttgccaatca attcggtgac tgagttatag atagctgtca tcttcaattc ctccttgtct
     7381 ttgctcttct tacttatcat aatacaatct gttcatttat ggcgagtgat tcgtctttga
     7441 ctagcaaaaa aaagacaaag atataccata tatggtaatt
//
LOCUS       DACDSY010000134         7440 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDSY010000134 DACDSY010000000
VERSION     DACDSY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.136"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..322
                     /locus_tag="IWK44_002377"
     CDS             <1..322
                     /locus_tag="IWK44_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550866.1"
                     /translation="HQFSVLVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIA
                     IRSDNKKWKKRYYILLLFLCFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFY
                     LVKK"
     gene            417..1301
                     /locus_tag="IWK44_002378"
     CDS             417..1301
                     /locus_tag="IWK44_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9550867.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1525..4692
                     /locus_tag="IWK44_002379"
     CDS             1525..4692
                     /locus_tag="IWK44_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550868.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4712..5257
                     /locus_tag="IWK44_002380"
     CDS             4712..5257
                     /locus_tag="IWK44_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP9550869.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5285..5740
                     /locus_tag="IWK44_002381"
     CDS             5285..5740
                     /locus_tag="IWK44_002381"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP9550870.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            5737..6495
                     /locus_tag="IWK44_002382"
     CDS             5737..6495
                     /locus_tag="IWK44_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9550871.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6581..7321)
                     /locus_tag="IWK44_002383"
     CDS             complement(6581..7321)
                     /locus_tag="IWK44_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9550872.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
BASE COUNT         2480 a         1295 c         1608 g         2057 t
ORIGIN      
        1 tcatcaattt tccgttttgg tgttaggcac actaattata gcggggattg tcgcatttat
       61 tattcaaggc cgtgacatat taacagtaca tcaagagttt ttcagttatg catttggtat
      121 tcctctgatc gctataaggt ctgataacaa aaagtggaag aaacgctatt acattctctt
      181 gctatttttg tgtttttatc tgccacctaa gatttttact ttattaggta tacaatgcct
      241 atcattttct tttatttcct atatcattgt tttaattttc ctcagtttca tagtatggat
      301 gttttatctt gtcaaaaaat aaatgaatgt ttcatgaaca gtacttcatc acagcctaat
      361 ttttttcttt atgctatcgt tatattaaag aaatcaatca gaagggaaag aaagtgatga
      421 aaaaagtttt attagtggta aaccccagtt caggcggtga acaagcaaag gaatttgagc
      481 aattagcgat agcaaaattg gaatcggtgt ttgatgaagt ggtcgtcttg catacaaaaa
      541 aagcagggga tgcaaaaaac tttactcgcg aagcggctac ggaagggtat catagtgtgt
      601 ttgtcatggg cggagatgga acagtcaacg aaggaatcag tggaatcgct gaacaagaac
      661 atcggccaaa tttcggattt ttcccattgg gtacggtgaa tgacttggct cgagcattag
      721 ggataccgct agaacctgag gaagcgatca atcatttttc gattgaatca gtcaagccat
      781 tggatattgg aaaaataaat gacgactatt ttatgaatgt cgtcgcaatt ggctctatcc
      841 ctgaatcaat caatgatgta gatgcggaga agaagacgaa atttggaaaa ctagcttatt
      901 tcatgtcagg aatcaaacag ttagcttcca cacaatccta ctcgtttcac gtagaagttg
      961 atggaaagaa ggaagaaatt gagagcagta ccttgctgat aggactaact aactcagtcg
     1021 gcggttttga aacattactt ccaaatgcaa aagtggatga tggtaagctc catttggttt
     1081 atttaaaaga ttcttctttg atcgatacgc ttaaaacatt gccagaccta ttaaaaggag
     1141 tagatgaatc tacgaaaaat ctagcctacc ttacttgtga gcaattagtt gtgtcattag
     1201 ctgatgagga agaattgacg acgaatgttg atggagatga aggggataaa ttacctgtga
     1261 caatcaaagt attgccctca catttacagg tttattgtta aaatggaggt tgtgaccgag
     1321 gagttggcgg gagaacaccg cttgttcggg ataagggact gtgacaagac ttttgtcaca
     1381 gccttttttt tgtacaaaaa agaaatttac acctaataga aaaatttttc cataaaaata
     1441 catattttaa atatgaaagt acaattggaa atagattaaa acgataaaat aaaattgtca
     1501 aaacgtcaga gaggatggtg gagaatgaat caatgtgtaa aaaagctgat tgcgtcgatt
     1561 ttgtttgtct tccctctctt gatggtgaca tccgtttttg ccgatgtaga gaaaacaaac
     1621 gaatcaacgc aatcagagaa tcaaagtttt gacaagacta ctttttcaga agaatcgatt
     1681 acttctgaaa ctacagaatc tcaaagtgag gcttctacta ataaaaggca agaagaaaat
     1741 aagacaagtg agacctcaga agtagctgaa caaacgtctg atagcagtga agcggcactg
     1801 gcaagagctg tttcagcgga tgggtacagc gaagctgcca caacagaaga actggcgcag
     1861 cttctcgcag acgaatcagt aactaagatc cgactgattc agcctttgac tttagacagg
     1921 gaattagaga tcaaacgaga tatcgttatt gattttggtg gattcgctca caatttcggc
     1981 acacaccata tttatatcaa tgagatacct cagtcgatcg agttccagaa ttttaaagga
     2041 acagctgcac atccaggtgg attgacacca aatatcgatg gaaatgccat tatcattgct
     2101 tatatgaccg atttttgggg aagcagatat cattttacgg gagaaataaa atttaccggg
     2161 acacttgatt tgtatgatgg gtccaaatta ggactgattt acgctcctcg tgcaactgtc
     2221 acattagatg gtgtttccgg tgtgttggat gtccaaccag tgaaggaagg actgaatgcc
     2281 gctccgggta aagcctattt ttgtagaagc tatcaattga atgtgatcaa tggtagtcaa
     2341 ctttatggac cgtatctagg gaaattctac ggttttctag attcaggaga tgagactggt
     2401 agtggaaaaa cagcgccagg aatccatatc ttaagcggtt caaaggtctc tcttgattat
     2461 gatcgggcag atggcgttag cgatgaagga gaggcagtcg atacgctacc tggtaatgtc
     2521 atgtttgaag tcagtggttc gggaagcgaa ttttcggtaa atacaaagat caatattacc
     2581 aatgacaaca atcgcggaat catccaaatg agaggttcag gatcgaaagt cactgtatcg
     2641 aatggtggga aaatcaccat caatacagca actacgggcg gatttcgctt gcaaggagat
     2701 tctagtcaaa tccatgtatc aagtggtgga caaatctcgg tcaaaatggc tggagatgac
     2761 gatcaacccg ggaacaatgg gatgcgtttt gtaggaaaag gcctttcttt gatcgtagaa
     2821 ggagccggat cagttatcga catcgataag cagagtgggc gttccagcgc agttcgtttt
     2881 gagaatggta cgcaaaagct gacagtaacc aatggaggct cattgagggt aagaaataca
     2941 ggtgatggca aatcttacgt gaacagactc aatcaaggaa atcaagcgat ccaattttat
     3001 gacgcttctg gttttctaga aagtccaggt tcagctgatt ttacagtcac tggagaaaaa
     3061 tcaaatatcg atatacgcgc agattccgga gcaactgtag atacaactgg agatattgat
     3121 ttaaatttta cggcaggtga aaaaacttat ttagtcatgg tcgggaaaac aggggaagat
     3181 aagaccggca tcatcagtgg ggatgtgttg aatgtggact tagaatctcc tacctatttt
     3241 gatttccaaa acaaacggat tggaagcgag accaatggca taggcggctg ggtattccaa
     3301 ggaaatggag attcttcttt aaagatcgcc cattcagaaa tcgctttatg gcgaaaaaca
     3361 ggcgcaacat tcgataactt tgaaaaagac ccggattatt tttctgatat gacagactta
     3421 tcgatcaatg ggaccgactt aggcaatttc gtatcaagcc ctagtgcagg tttaacaaat
     3481 gaatttgctc agacaggtgc cggaatgaaa aattataatc gaatcagtgc caataatcag
     3541 ctcccgatga ttgaatcctt aagagtgcca acgaatgcag ataaaaaaat ctatgggcat
     3601 gtcgttgttc ccgaaggtgt cgaggcaaag ccaagagatg cttggacagg cgaagtagaa
     3661 gtgactttac gtattactta cgcagatacg acgaaatcat ctgtagaaat tactgctctc
     3721 acgcaaggac aaacagatga aaatgaaggg gcaggatata acccatgggg agaaggcgaa
     3781 caaggcggct tgttcgaagt tcaagtaccc aatgatgaga gattaaaaac ggaggatcaa
     3841 gtgcaagttg ttgctgctaa aaaagtaaga ggtggccaag aagtgacaga actggactct
     3901 gccaaaacga cagtcgatgt gattcctcct gaacctgcta atttaagtac cacattgatc
     3961 aatgcagctt ctcgcacgat cacaggtaca ggtgaagcag gagccttggt ttcattgaaa
     4021 aaaaatcaag ctgttttgtc agaaacaact attgatgata caggggaatt tgttctttct
     4081 atcccagaaa atcaactttt tgcaggagat gtattagaaa ttgtattaaa cgaccaagca
     4141 ggagaagcta ctgctaaggg tgtgcagaac aagccaagta ccaacgatga aattgggaat
     4201 cacaatccaa gtgatacgcc agttgtttat catgatgccc cgccttttgc acctgctacg
     4261 aagatcgtag tagaaggtgg tctctcattt cttgcccctt ccaaactcga ttttggtcag
     4321 atcaaagcaa ctggtttgac acaagaagca tttggccaaa tggatataca ggaaaaatta
     4381 tggatttatg atcagcgaga aacgaaaaat ccgtggatct taacgataaa attatctcag
     4441 cctttcacaa atgatgcagg attttctatg caggatgtcc tatatttcaa gaaaggagag
     4501 gagtatcaat tagtgaatga agaagaactg gaaattgtca gaggacaaaa ttcaacgaat
     4561 gtgcaagcag attattcaga atatttaaat aacggtcggg cgtttaaact tgtcttaacc
     4621 aaagaaaatc agatagctgg taattactca gcagagataa catggacatt agcagatgcg
     4681 ccgacagaat aaagatcaag gaggatgatt gatgaatcga atatggaata aaagtcatcg
     4741 aaacaactgc tctttactta tgatccttct ccttggattc atcctttggc agacacctgt
     4801 ccatgcctcc gaattcaatt ttgcggtaga ttttgaatta ccggctaatc agattgataa
     4861 gaacaaaaca tatataaatg taaggatgga gcctgggaaa acacaggaaa tcccctacac
     4921 ggtctttaac cgaacagata aatcagtaac agttgagaca ttgatccact ccgctacgac
     4981 gaattcaaac ggtgtgatcg aatatggaga atcaaaaagt aaatttgatg aaacattaag
     5041 ttatgatatg aaaaacttag caaaaacagt gggagaaata actgttcctc ctcaaagtgc
     5101 ggtcactgag acactttcac tgaaagcgcc agaaaaagag ctggctggtg tggtcgcagg
     5161 agggatcacg tttcaagaaa aaaaagagaa cgtttctaac aaaagtacag ttggtgtcgt
     5221 caatcagttt tctcaagtga aagcaatctt gatttgaaat agcgaaaaag agtcttcccc
     5281 agatttgaaa ttaaaaaaag cttatgcgga tcaaagcgat ggaagaaatg tgattaaggc
     5341 gaatcttcaa aatccaaatg cttcttatat gttcggcttg actacatcgg ttgaaatcta
     5401 caaaaaagaa acaaaagagc cttacctttc aataaaaaaa gaaaaaatag atgtagcacc
     5461 caatacaaat tttgatttac ttgtcccatt agaaggaaaa aaattagctc cgggacatta
     5521 tcaagcggtt gtgaagggtg cttggaaaga aaaacgctgg aatctccata ccgattttga
     5581 aataactgat gatgcagcgg accaattgaa tgaaaaggat attgatttaa aaaatcagcc
     5641 agaaacattt gactggcgat ggctgatact tgttggagtc attcttattg gcttatttgc
     5701 tggattattt attttaaaag aaagaaggaa aaagaaatga aaaaaacaat tgcattaagt
     5761 accgttctag ctggaagtct attattatgt acacaaagtg tttttgctga gaataagagc
     5821 tcagccacta cagaaggaac gatttctgtg accaaaccag atgctgcaaa tcctgaagac
     5881 gttgttccag ttcctcctgt tgatccaaat gatcctgata ctccttttac acctgacgaa
     5941 ccaaatcagg gaacatccgg attactgacg attgaccaag ctccagatct tgattttgga
     6001 acaatcaagt taggtacagc aaaaacagta tatgctgcat tacaaagcgg aacggacagt
     6061 actggagcac aaacacaagt acagaatttc gtacaagtaa cggataaaag cggaaattat
     6121 gccggttgga aattatcagt caaacgaaca gaatttattg gtacaacatc tgatacgaat
     6181 aaactaactg gttcaaaggt gaaattcaaa aacgccgtga ttcgaaatag cgcaactaat
     6241 agcagtggaa tgccatctac cgtttctgga actggcgctg aaggaatcga aatcccagca
     6301 gatcaggcag tagatctagt aactgcagga gcaaatgaag ggattggcac atggatttat
     6361 tcattaggag agaatgtgga acaagggaga caatcagtgg aattatttgt tcctgtatcc
     6421 aattatgctg tagacaacta cgtttcaaca ttcacatggt cgttaacaga tgctcctaca
     6481 gaagaggaag cgtaaaatca tatcaacgat ttcagggagt ggagccgatt caaggcccac
     6541 tccctgaaat ctgctatgct tacgtctcag ctcttattca tcaactcgaa gcagtttttt
     6601 ggaagtcatg gaaacgttcc tttgtctttt caaagatctt tcgaccaacg agtaaggagt
     6661 caccgttgaa aaattctaac acataatgag cagaatcaat tttctgcaat tgcgataagt
     6721 tgatacagta agattttaaa agtaaaaagt aagcatactt ttctaactct gtcttcaatt
     6781 gttttaaaga agtattcact aatttatctt ctgtagttgt tttgaccata acttggtttt
     6841 tcaaaccttt gatactttca atatataaaa tttcccgagg atacaggacg agacttccac
     6901 ctttttgatc aataaagatt ttatcgacat ttacgatctg ctgttctttc caattgatct
     6961 gttctagcac ttgacgaatt tgttcagata aattcctgtc ttttctgata tatccaatcg
     7021 gaaggatttt ccgattgatg atctctactg caaaattcgt ataagcggtg aaaaaaacaa
     7081 tattactatg tgcatttttt ttacgtattt gttcggcgac atcaatacct gtgagtgatt
     7141 gcttcaagtc gatatcgata aagaaaacat cgtgattatc aatagtgccc aactggatca
     7201 actgattgat ttgagagata gaagtgagct gcacgatatt ctcgatatga tgattgacca
     7261 attctctgat gatccattcg ctgtgaaact ggttatcttc tatgataaaa tactttttca
     7321 tatgcaggct cctattaaaa aaatatgtgt acattttgaa acaaaacaat ggaggaataa
     7381 gatggtcgca acagtgacac tttatttgac ctttactttg attttagatt taggtatcta
//
LOCUS       DACDSY010000135         7353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDSY010000135 DACDSY010000000
VERSION     DACDSY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.137"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            132..3659
                     /locus_tag="IWK44_002384"
     CDS             132..3659
                     /locus_tag="IWK44_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9550873.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3656..>7353
                     /gene="addA"
                     /locus_tag="IWK44_002385"
     CDS             3656..>7353
                     /gene="addA"
                     /locus_tag="IWK44_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP9550874.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRV"
BASE COUNT         2529 a         1269 c         1544 g         2011 t
ORIGIN      
        1 ttttgcgtat acttaaaaaa gtagaaatat cagaacctta aaaggatata atttgaagaa
       61 tatagaacaa atgttcagta ttttttgata taataaagga gcaccgtatc atgtgttgaa
      121 ggaggaaaag catgtcttta caatttattc ttggaaacgg gaaaaaagat caccgtcaag
      181 tcttgatcga cgaagcatcc gcttggctcg accaggatgc aagtaaccaa gtatttttcc
      241 ttgttcccaa ctacagcaaa ttcgaacagg aacaagaaat tttagcggag atgcgaagaa
      301 gaagcggaaa aaaaactttc agcacaacaa acgttcaagt atttagtttt tatcgtctgg
      361 cttggtattt gctgcaacag acaactcttc ttacaggaaa tgaattatca gaatcaggat
      421 ctgcaatgat cttgcgacag attttagaaa aaaatgagga agaattaaca atctttcgcg
      481 gagaaatcaa taaaagaggg tttattcgtc aattacaaga actctacagt gaattgcgta
      541 caggaaatat tcaaccagaa gacctgatgc ttttatttgg tgattcacct aatgcagaag
      601 accagcaact taaaatgaaa gacttgaagc tgattttttc agcttatgac agcgaattga
      661 ctcaacgtgc actaacaaat gaagatgcac tatctatact agcaaattat atgcagacac
      721 aagatttcaa caacgtcaaa tttattgtca gtggtttttc tcgaatcaat ggtcaagagt
      781 atcaattact gcaggcaatg atggaaaagg ggcaattggt tgtcgattta ttgctggatc
      841 gtccttatac agcggatctt ccagaagtac ttacgctctt tcatgagact ggaaagcttt
      901 atttccgtct ttttcaaagt gcaagagaac ggaaagtccc tatattagtg gaccgatttg
      961 caccagagca gactcgtccg acagagcttc aactgctgca aacaatgtgg gaagagacat
     1021 ctagccaaaa gaaagcggca gtacacaaaa tagggggaca agagcatctc catttgtggg
     1081 aagctgaaaa tccaactgaa gaagtccgac aattagctgt tgaaatcaga cgactagtca
     1141 gtgaagaaaa ctaccgttat cgtgatatcc aagtgctgac aaaaaatatg tctttatatg
     1201 gaaatatcct tcggccgatc tttcaagaaa tggcaattcc tttttatatc gatgaagaac
     1261 aaatgatgga aaatcatccg ctgatcgaat ggatcaacag tctgttcgct cttgaccgct
     1321 attcttatcg gctaagtgat attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg
     1381 aagcagaaac cgaacttgat gaatggaaac aagagcgaga tgaattcagg aaaaaactgg
     1441 acatcactga aaatgtagca cttgcttaca attatcaagg aacttattgg actcgtgaaa
     1501 aagactggca gtttgtggcg tatgattttg aagcagaaca gattgaagac gtacagtatt
     1561 tggaagaaca gtcaaatacg attagacgat tatttcagaa agctgtacca gtatttttga
     1621 aaaaaataaa gaaaagcaaa acaggacaag aggccgcaaa tgttttttat caatttttga
     1681 tccaaagtgg cgtacagcaa caattattgt tttggcgtaa tcaggaagta gacagaggaa
     1741 acttagaagc ggcaagaaac catgagcaga catggggagc gctgattgat ctgttggatg
     1801 aatatgtagt aatttatggt gaatcggctt ttgactggtc actgttccaa gaaatcatta
     1861 caagtggtct agaaaatcta agttatggta aaatccctac cgcaatcgac caagtgcgaa
     1921 tcaatcgttt ggaattagtt cgaccaaatc aagctgccgt cacttttgca ttagggttga
     1981 atgatcaagt gtttccagac cgaaaagaga caaaaggatt gttgtccagt gaagaacgag
     2041 aagtattaaa tcaggccttg ccagatggac agtttttatt cgacccatca aaggaaagta
     2101 tctcttttga gcctttccaa gcctatcttg tttttctttc cggaacagat cgattatatc
     2161 ttagttatgc acaaagctat gacacagatg gttcgctgaa gatgtctcct tatttgcgta
     2221 gaatcacaga atatttatct gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg
     2281 accctaattg ttatgtaggg acttacagaa gcggaatcaa tacagtcaat aggatttatc
     2341 gattagcttt agatgaaaaa aaacctgtac caaagcactg gcaaaagctc aaagaattag
     2401 tactgaattc gtcatggcga aattttgctt tacagatttt tgaaagtcag gaacatttaa
     2461 atgttcctgt ttcgatgcca gaaaaaatgg cggaagagct ttatggaaaa gagatttata
     2521 cttcaatttc acgtatggag aatttttata attgtgaata caaatacttc atgcaatttg
     2581 gtttgaaatt gaaagaacga actgtttatg gtctaactcc agcagcaact ggtgattttt
     2641 atcatgaatc attggatcgt ttcttcaagc tactgttttc aaaccaatta tccttagtga
     2701 atatgtccga tgcgcagcgt agagacttta ctgaaaaagt gctgcaggaa gtattcggag
     2761 aacttcggtt tgagatattg gatagttctg caagaatgaa ctatatccgc taccagttag
     2821 gacagacgat tcaaaaagtg acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc
     2881 atcctcttca gacagagatt ctatttggtc agattgctgg agcaaaaggt atcacaggtc
     2941 tggaattacc gttgagtaat ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg
     3001 atgtagcttc tgaacaagaa gatacttggc taagcgtcgt tgattacaaa tctagcggac
     3061 gttcattcga tgtcacagaa gcttattatg gcatggctat gcaattattg acgtatctgg
     3121 acgtagcatt gatggatgcg gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat
     3181 catattattt acatgttcat aatcctgtat tgacggagac agatgatgta gagaagaata
     3241 cattgaaaaa atttagttat gatggcgttt ttgtcgatga tcccgctctt ttagaagtac
     3301 tagatcattc actggaagca aaagaacaat cactgatcta tccagtgaaa aaaaatgcga
     3361 aagatgaata ccaaaaaggt agtttcagta aagacaaatt tttcacggaa gaagaactgc
     3421 aactattcat ggcgcataat cgggaaaata tgcggttagc tggagatgcg attacttccg
     3481 gagaaatcaa actaaatcca gcttataaag ataaacagcg gattgcctgc gaattttgtc
     3541 cattcagaag catctgtacg tttgatgtga tgctgaaaga aaataattac caccgtctgg
     3601 agaaaataga taaggaagaa gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa
     3661 tcaaattcct ttaaaaccgc aaaatgaacg attcacagat gatcagtggc aagcgatttt
     3721 tgatcaagga gataatctat tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct
     3781 tgttcgaaga gtgattgaaa aactaaaaat gggttttgat atcgatgaat tattgattgt
     3841 gacatttact gaagctgccg caagagaaat gaaagaaaga attcaagagg cattacaaga
     3901 atccgtcaat agcgaaagtg accctgtacg tagacagcat tttactaagc agttagtttt
     3961 attgcccaca gcgaatatct caaccttaca tgctttttgc ctgacagtta ttcgacgtta
     4021 ttattactta attgatattg atccagtctt tcgcatgtta acagatgaaa cagaaacgat
     4081 cttgatgaag gaggatgtgt gggacgagct gagagaagca ctttatgcag aaaatgatga
     4141 acgatttttc caattaacaa tgaatttttc taatgatcgt tcagatgacg gcttgacgaa
     4201 tctggtgttt tctctttatg agtttgcccg tgccaaccct gacccccaaa aatggctaga
     4261 acagttaagc gataattatc gtttgcctga ggggctagcg aaatctcgat tatatcaaaa
     4321 acaaatccgt cctttagttt tggcagatat ataccaatgt gtacagttat atgaacagat
     4381 gactcagtta gcacaaggtg aaggattaga aaaaatgcat gagcaagtag ctggagaaca
     4441 acagcagatc aagaatatct atgaagcgtt ctcgcaagat cggttagaag aagcttatgc
     4501 tggtttggaa caactaactt tttcaacttt taagagcagt cggaaagcag aattgaaaga
     4561 aatttccaat gaagtcaagg gcatgaggga taaagcaaaa aaactgattc agcagataag
     4621 taaaagttat tttcctgttt ccccaagcca aatggaagaa ttaacggaca aagcactacc
     4681 gttagtcgaa gaaatgacaa aagtcacgca aagcttcatg gacggtttca gtatgcgtaa
     4741 acgggaaaaa ggtgtgctgg atttcaatga tctggaacat ttagcgttgc agattctcac
     4801 agaaaaaacg aaagatgtat ggctgccaag tgaagcttca aaacattatc gaaaaaaatt
     4861 caaagaagta atggtagatg aatatcaaga cgtgaaccaa ttacaagaag ccattttata
     4921 ctggctaaga gaacccgatg atacaaaagg aaatatgttc atggttgggg atgtcaaaca
     4981 atccatctat tctttccgtt tagcagatcc tagtttattt atcgggaaat acgagaattt
     5041 ctcaaagaaa gaaggcggac gtcgaatcgt tttggctgag aacttccgct ctagaaaaga
     5101 agtcctttct tttacgaatc ttatttttga acaactaatg gatccagcag tgggtcaaat
     5161 caattatgac gaagcagcca aactgatcca aggattttca gactttccag agaatgaaca
     5221 gtttgagccg gaaatcatga tctatgaaaa agaacaggaa gaatcagaga ttgagattcc
     5281 gacggatgat atactggaag acaaaacaga aggcgaactt tttatgacag gactaaaaat
     5341 tcgtcaatta attgattcct cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc
     5401 gatagagtac aaagacattg ttctcttgac acctacaaag aagaacaact taacgatttt
     5461 agagattttc aaaacattgg atattccatt agagatgaat gatgctcaaa actattttca
     5521 agcgacagaa attcgcacga tgatctcgct gctacaatta atcgataatc cctatcaaga
     5581 tattccgctt gcggctgtat tgcgctcacc gatcgtgggt ctgattgaac cagaattagc
     5641 cagcattcga ttagctaacc gtgcacatac ttactatgat gctgtgcttg cttatcaagc
     5701 atcaaacgaa gatgaattag cagctaagct ggaacatttt ggcaagcaac tagagcattg
     5761 gcgagaactt gctcgccgtt cttcgatcac tgacttgtta tgggatatct attatgagac
     5821 gggatatcta gagtatgtgg tcggtctgcc agcaggggca cagagacaag ccaatcttta
     5881 tgcattggtt gatcgtgcca aagcttacga gcaaagttct ttcagaggat tataccagtt
     5941 cgtgcgtttc attgaaaaaa tgcaagaaaa agataaagac ttagcagaac cagtcatttc
     6001 aatagaagat aatgctgttc gcgttatgac gatccatgca agtaaaggct tggaattccc
     6061 agttgtattt ttgttagata tgacaaagga gttcaactta caagacttgc gcaatcggta
     6121 tgcttttgaa gaaaaacttg gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt
     6181 atatgatacg ttaccgtttc aagccatcaa attggcaaag caaaacaaat tgttgtcgga
     6241 agaaatgcgt aaattgtatg taggattgac tcgggcagaa caaaagcttt tcattgtcgg
     6301 ttcctataaa aataaagagc aaatgatcca aacgtggtct gaagcagctg atcatgagga
     6361 gttagttttt gatcctgcgc tacgtttgaa gggcagaagc agcttgatga attggattgg
     6421 ttacggattg atccgtcatc ctgaaatgca gaaatactta gaggaagaaa tttcgacttc
     6481 tcttcttcaa cattcaaatg cacagttttc tatttcatgg atgaatcagc aatcgatcat
     6541 tgaacaaaga cagttgcttg ctgaaaaaga actggttaac ttagatcagc agatgaaaga
     6601 agatgaaact ttattagcag attcgttaca aaaacgtctg gcatatgaat atccttatca
     6661 agcttcttca caaaccacaa gttatcaatc tgtatctgaa atcaaacgat tattcgaaga
     6721 tccagatgat acacaagaat cacgtttgac tctggaaagc agtcagaaca aagcagcatc
     6781 ccgtcaattt cgttatacgc aagaacaact agcagaacca aaatttttgc aaaaagatcg
     6841 gcaagtgtca gcagctgctg ttggaactgc tacgcatgct ttgttacaat tactgccatt
     6901 agagatgcca actacagaga gtatccatca aaaattacag gaattggtaa ggaaacggtt
     6961 agtagacgaa aaagtcgcga aaaaagtaga tgtttcttct attatatggt tctttcaaac
     7021 agaacttggt caacagctga ttgccaacaa agaaaacgta aaacgagaac aacctttttc
     7081 catgctgctt cctgctgacg aagtatttca agactatcca aatcaagaag atgaactatt
     7141 aatccacgga atcgtcgatg gatacctaga agaaaaagat catctcaata tctatgattt
     7201 taaaacagat tttattttac atccggatga tccagcagaa atagatgcaa tcgtccaaaa
     7261 atatcaagga cagctaagac tttaccaaca agcaatgtca gaagctttga ataagccagt
     7321 agaaaatgtt ttcctaattt tgcttcgagt gaa
//
LOCUS       DACDSY010000136         7271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDSY010000136 DACDSY010000000
VERSION     DACDSY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.138"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..106
                     /locus_tag="IWK44_002386"
     CDS             <1..106
                     /locus_tag="IWK44_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9550875.1"
                     /translation="NNQFGYANAIALVLFILIGIVSLAQLKLSKKFEI"
     gene            118..948
                     /locus_tag="IWK44_002387"
     CDS             118..948
                     /locus_tag="IWK44_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9550876.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            988..3171
                     /locus_tag="IWK44_002388"
     CDS             988..3171
                     /locus_tag="IWK44_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9550877.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            3232..4452
                     /locus_tag="IWK44_002389"
     CDS             3232..4452
                     /locus_tag="IWK44_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP9550878.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            4449..5909
                     /gene="gtfA"
                     /locus_tag="IWK44_002390"
     CDS             4449..5909
                     /gene="gtfA"
                     /locus_tag="IWK44_002390"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP9550879.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            5986..7092
                     /locus_tag="IWK44_002391"
                     /pseudo
     CDS             5986..7092
                     /locus_tag="IWK44_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         2472 a         1161 c         1401 g         2237 t
ORIGIN      
        1 aaacaatcag tttggttatg cgaatgctat tgctttagtc ttatttatct taattgggat
       61 tgtttcactg gcacaattaa aattatcgaa aaaatttgaa atctaggagg tttcccaatg
      121 aaaacatcaa aagaaaaaaa tatttctaaa tacttgcttt tattattagg gggcttcatg
      181 attttagttc ccttagtttc aacgctattc ggttcattta aaacaacacc cgatattatg
      241 aataatttct ttcgttttcc taatccagcg actttggaaa atttccgaag attgttagcg
      301 gatggaattg gtcattactt ttggaattcg gcaatcatca caatcattgc agtagttgct
      361 gtgacagcaa ttattcctat ggctgcttat tcactagcaa gaaaaatgac tcgtgggaaa
      421 gcatacggat tgatgtattt atttttaatt ttaggaattt ttgttccttt ccaagtaatt
      481 atgattccga ttaccgttat gatgagtaaa attggtttag caaatatgtg gggattaatc
      541 ttactctatc tcacttatgc cgtaccacag acactatttt tatatgttgg ttttattaaa
      601 ctaaatgtgc ctatcagtct ggatgaggct gctgaaattg atggggctag tcactttaca
      661 acttatttca aaattgtttt cccaatgtta aaaccaatgc atgcgacgac tattattctt
      721 aatgctctat ggttttggaa tgatttcatg ctaccattat taattttaaa tcgtgattct
      781 aatatgtgga cactaccatt gtttcaatat aattatacgg gacaatattt taacgactat
      841 ggtccaagtt ttgcttccta tacagtagga attgtcacga ttacactagt ttatcttgct
      901 ttccaaagaa gtattatagc tggaatgagc aatggagcag tgaaataacc aataaattat
      961 tctcattata aaggagttca taaacttatg attatatttg acaatcacac acaaacattt
     1021 catttacaaa ataaaaaaat tagctatcta ttaggaatcg aagaaaataa ttatttaacg
     1081 catctttatt ttggaaaaaa gattacgcac tattctggtg ggtatcgcta cccccgaaca
     1141 gatcgttcct tctcacccaa caatgcaaat gcgcaagatc gtttattatc tttagacaca
     1201 ttaccattag aatttccagg atatggacat ggtgatttta gagaaccggc gcataatatt
     1261 aaattaggaa acggtagtcg tgtaaatgat tttcgttatg atagctacga aattcaaaaa
     1321 ggaaagccca gcttagcggg attgccagcc ttatatacgg aagatgatga tgaagctgaa
     1381 acgctgatta ttcaattgaa agacgcgatt agtggattga ctttacagtt gttctacaca
     1441 atttatcaaa atcacagtgt aattattcgt tctacgcaat tagtaaataa tagtgatgaa
     1501 acagtggaga ttaaccgctt agctagtcaa tcaattgatt ttccaaatcg tccactcgag
     1561 ttaattcatt taaatggtac ctgggcaaga gaacgtcaaa tgactagaga aaaaattcat
     1621 acagggataa aagtattaga tagtaaacgc ggatcaagca gccatcaaca aaatccgttt
     1681 gttgctttag tagatgccaa aacagatgaa tttcaaggag aggcatatgg atttaattta
     1741 gtttatagcg gtaatcacga aacggtgatt caaaaagatc catttgacca aacacgggtc
     1801 attatgggaa ttaattcatt taattttggt tggcaactaa aaactggcga ctcatttcaa
     1861 acgccagaag tcgtgatggt ttatagtaat gaaggtctga atacgttatc acagacgtat
     1921 catgaattgt ataatcaccg tttaattcgt ggaaactatc gtttaaaaga acgtcccacg
     1981 ttaatcaaca actgggaagc aacgtatttt gattttaacg aagaaaaaat catgaatatt
     2041 gtcgatgatg cgaagaactt gggcattgaa atgtttgttt tagatgatgg ttggtttggc
     2101 ggtcgtgaaa atgacttcac ttcattaggt gattgggttg aaacagaagg caaacttgct
     2161 tgtggtttgg aaaatttagc caagcaagtg catgataacg ggatgaaatt tgggatttgg
     2221 tttgaaccgg aaatgatttc tgaagatagt gaactatttc gagcacatcc agattgggca
     2281 ttgggtattc caggtcgtga ccgctcaatt agtcgaaatc aatatgttct tgatttttca
     2341 agacaagatg ttcgtgataa tatctatcaa caaatgacgg cgatttttga tcgtgtaccg
     2401 attgattatg tgaagtggga tatgaatcga aatatgacag aagtctattc tgtcttgttg
     2461 gactcaacga tgcaaggtga agtggctcat cgttatatgt tagggttata tgaattttta
     2521 gaaaagttaa caacgaatta tccaactatt ttatttgaaa gttgttcggg tggaggcggt
     2581 cgttttgatg caggtttctt gtattacatg ccacaaactt ggacgagcga caataccgat
     2641 gccgtggctc gtctgaaaat tcaatatgga accagcttag tgtatccaat ttcaagtatg
     2701 ggtgcgcatg tttcagcagt cccaaatcat caaacaggaa gagaaacgag tttaactatt
     2761 cgtggcaatg ctgcgatggc tggtgttttt ggttatgagc tagatctaac gcaattaact
     2821 tctgaagaaa aagcagaaat taaagaacaa gttgcctttt ataaaaagca tcgccaactt
     2881 ttgcaatatg gaacttttta tcgcttggaa agtccgtttg aaggcaatga tactgcatgg
     2941 atgtttgtct caaaagacca aacagaagcc atggtctttt atttccgagt attagcagaa
     3001 gctgcgcatc cattagtcac attgaaatta gcaggattag cagaagaagc aacttatcaa
     3061 atggaagatg taacatttgc tggcgatgaa ttgatgaatc tcggttttta tattgactca
     3121 gaactacatg gtgattatgc gacacaacgc ttttatttga aacagatata gtagcttgaa
     3181 tttacaatat aagtgcaaca ccaaaagaga ctcattccaa acgaagaaaa tatgttttat
     3241 ttacacgcat ttactaaaaa gcagcctgat ttgaattggg aaaatgaaga agtacgtgaa
     3301 gaaatctatg caatgattaa ctattggtta gataaaggac taggcggatt tagaatcgat
     3361 gcgatattaa acatcaaaaa aaaactagag tatggcatct ttgaatctga tggagaagat
     3421 gggttagcct tcattgggcc ctggattctt aatcagtcgg gcattgaagt gtggttaaaa
     3481 gaaatgagag agcggacatt taaaccacat aatagtatga cggtagcaga agccgatgtt
     3541 cctaatgaac gtttagatga atatattggt gaagatggct attattcgat ggtttttgat
     3601 tttagttata cggatattga tgtacctgaa actggtgaat ggtttaaaga tagccaatgg
     3661 acatggacgg atatgcgtac caatattttt acgaatcaat tagttacgca agataaaggt
     3721 tggggcgcct tatatttaga aaaccacgat caaccacgtt caattaataa atatattcca
     3781 gaagaatgga tcaatgatta tagtaaaaaa atgttggcga cgctttttat gttactaaga
     3841 ggtacccctt ttatttatca aggacaagaa ctaggaatga ccaacataga aatggcatca
     3901 ttagaagatt ttgatgatgt agctacgcat tctcagtata aacgggcatt agaatatgga
     3961 ttggcaccag agcaagcatt gaaagctgtc aataaacgaa gtcgggataa ttctcgcaca
     4021 cctatgcaat ggaatacgca aaaaaatgcc agattctcaa cttctgataa tgtatggtta
     4081 aaagtaaatc ctaattatcc agaactcaat gcagaagctc agttaaccaa tcaacagtcg
     4141 gtattcaatt attatcatca attaattaaa ttaagaaaaa atagtgtata caaagacgtg
     4201 ctaatctacg gacaatttct accagtgaaa gataaaaatg aacatatttt gctttatgaa
     4261 cgccaattag aagataaaat cgtattagtt atcttaaacg ttacagcaac agaacaagaa
     4321 tattatgtag atgaacagta ccaacaagta ttaatcaata attatgaaga tgttttatta
     4381 gatcaaagtc gaaaattgcg tttacgaccg tttgaaagta ttgtattagc taattatgga
     4441 ggacaattat gaaaattaaa aacgaagcaa tgctcatcac ctatgcagat agtttaggaa
     4501 aaaacatggg cgaattaaaa caagtcttag acaaacattt acaaggtgtt attggtggtg
     4561 tacatctgtt gccttttttt ccatcgacag gtgatcgagg ctttgcgccg agtgattata
     4621 cgacagttga tccagctctt ggaacatggg aagatgtgga agcattaggt gaagattatt
     4681 atttgatgtt tgattttatg attaatcaca tttctcgtga atcaaaattt ttccaagatt
     4741 tcaaaaaaaa ccatgagcag tcaccataca aagaaatgtt tatccgtatt catgaatttt
     4801 tcccagaaaa tcgaccaaca caagaagata ttgatttaat ctacaagcgt aaagacaaag
     4861 cgccgtttca aacggtagaa tttgcggatg gtacgacaga agaggtttgg aatacctttg
     4921 gggaagaaca aattgattta gatgtgacca aagaagttgt gaaagaattt attcgagaaa
     4981 ccatcaaaga tatggcgaac catggctgtt ctttaattcg attagatgcg tttgcttatg
     5041 cgattaaaaa attagatacc aatgatttct tcgtggaacc agatatttgg gaacttttag
     5101 acgaagtgcg tcaagaagca gcgaaatatg acgtcgaatt attaccagaa atccatgagc
     5161 attattccat tcaaatgaaa attgccaatc atgattatta tatctatgac ttcgccttgc
     5221 caatggtcac tctgtattca ctttatagtg gaaaatcaga acgtttagcc aattggttga
     5281 aaatgagtcc gatgaagcag tttacaacat tggatacgca tgatggaatt ggtgttgtgg
     5341 acgctcgtga tttattaaca gatgaagaat tggattacac ctctgaggaa ttgtataaag
     5401 tcggggcgaa tgtgaagaag gtctattcaa gtgccaatta caataattta gatatttatc
     5461 aaattaacag cacctattat agtgcattag gcgataatga ccgtagctat ctgttagcac
     5521 gtgcgattca agtatttgcg ccagggattc cacaaattta ttacgttggg ttattagcag
     5581 gaaaaaatga tattgaactg ttggaagcaa cgaaagaagg gcgtaatatt aatcgtcatt
     5641 actatgattt agccgaaatt gaagaagaag tgcaacggcc agtgattcaa caattatttg
     5701 cacttttaaa atttagaaat gaatctgcgg cgtttgattt ggatggaacg attgaagtag
     5761 caacgccatc agaatcagaa atagtgatta cacgtaaaaa taaaaacggt agtgatagtg
     5821 caacattaac ggctaacttg gatacgaaag aatttatcat tgagtctaaa gatataaaaa
     5881 ttttcactag tgttgcatta aaattttaac ctattccata gaacaaaaaa tcctttatac
     5941 tagcacttgg atgacttatc caagaccaat aaaaaggact catacatgga taagtataaa
     6001 acaaatacag catttaataa atggttttct gccataaaat taggaaaact ttctgtcaac
     6061 tcccaaaaat gtatttttga ttacaattcg tatcgtaaaa aactgtcttt tgaagctgtg
     6121 ctcaagctct ttctctacgc agttaatgaa gaaaaagaaa gtttgcgtga tttgagtaca
     6181 gctcttatcg accgatcgct ccaactagaa accgatgtgt catcgattag tcatacccaa
     6241 ttgtctcgag catttaatgc acttgaacct aaagtgttag aagaaatttt tcaacaactg
     6301 ttagaaaaag ttcaacatca aatcaaacca accaagcgaa atcatctgta tctgattgat
     6361 tccagtacgt tttcgcttag tttaaaacgt cataagtggg cgactttccg tcaaacaaaa
     6421 tcaggtgtca agttacactt aaactattgt tatatggacg atactacgat gtatccaact
     6481 gattttacct taacaaatgc ccatgaacat gatctcaatc aactaccagt attggtgaat
     6541 caaccagagg caacttacgt gtttgatcgt gggtattagg actttgaaaa gatggatcag
     6601 atgcattggg atggctattt cttcgtcact cggattaaaa aaaacaccaa agttcacgtg
     6661 atagatactc tggaaacatc gccagaaaca gaaattctac gagacgagct ggttcgttta
     6721 ggatctaaaa cctatctaac cgccaatttc cgactagtca ctgttcaaga taagaacgga
     6781 cgtgtgtttc aatttatcac caatcgaatg gacgtctctt caaaagaaat ctctgatatg
     6841 tatcacgctc gttggcaaat cgaattgttt ttcaagcaca tcaaacaaca catgaccatt
     6901 aaaaccttct tttctcaaag tgaaaaaggc gtgcagaatc aattgatttt aacgatgatt
     6961 tccgctctcc tcacgtttct catcaaattg gagacgaaaa cggaaaaatc agtgttccaa
     7021 atcaaacgat tctttcgtta tttactgttt cagccctttg aatgttgtct agaaaaatta
     7081 attcccacat gagaaaaaag aaatttggct cgttactttt ggttttcagt tgaataacag
     7141 ataaaaaatt ttctggaaaa agtcatcctc tgcgtcagca cagttttttt tgacttattt
     7201 tcagaggaaa tagttttcag aatattctaa aatgttttat gcaacactag tgatattttt
     7261 tagaattaga t
//
LOCUS       DACDSY010000137         7177 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDSY010000137 DACDSY010000000
VERSION     DACDSY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.139"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            7..1053
                     /locus_tag="IWK44_002392"
     CDS             7..1053
                     /locus_tag="IWK44_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9550880.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            1055..3208
                     /locus_tag="IWK44_002393"
     CDS             1055..3208
                     /locus_tag="IWK44_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9550881.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(3681..5132)
                     /locus_tag="IWK44_002394"
     CDS             complement(3681..5132)
                     /locus_tag="IWK44_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9550882.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(5129..6853)
                     /locus_tag="IWK44_002395"
     CDS             complement(5129..6853)
                     /locus_tag="IWK44_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9550883.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(6846..>7177)
                     /locus_tag="IWK44_002396"
     CDS             complement(6846..>7177)
                     /locus_tag="IWK44_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9550884.1"
                     /translation="QIKGLIFLAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLK
                     LSFISVFSSFSSFLKIIIGSGIILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELD
                     KQLESYE"
BASE COUNT         2109 a         1409 c         1288 g         2371 t
ORIGIN      
        1 gtttatatga ctcaaccatt gtaccagaca atatttaatg atttgcaaac ggctatttta
       61 actggtgaat tacctgtaga cagccaacta ccaactgaaa aagaactttc acgtacctat
      121 aaagtaagca gaatcacttc caaacgtgca ttgactgaat tagagcaatt aggacttatt
      181 tatcgcgttc gcgggaaagg cagtttcgtt aaatccgctt ctccggaaca gattcttgct
      241 tctcctacaa aaacacgacg gattctgttt cttcttccat atgtcgctga tttgtctgtt
      301 ggtgatttta gcaagggatt gaaccctgtg atgcaagata aacagattga tgtcatgatg
      361 acgacgcttg actttctaca aaataaaaca gcaaaagagc taatcaatga atttgacgga
      421 ttgatttatt atgctcttga tacagaacaa catcttgact tactttttga gctttcattg
      481 aaagaattcc ctgtcgtcat attagataaa aaaatctatg agttaccttt tcctaccgtt
      541 ctttccgata atttccaagg cggtgcgctt gctgcctctt atttgatcga acagggacat
      601 tcaaaaatcg cttatttgtt tggtacgcag actcatccac aatccgttcg acagcgttac
      661 cttggttacc ttgaagcatt ggacaaagct ggtttgactt tccacacttc actggatgac
      721 aaaagcgcga cattagatga attgctttct tatatcacta agtatgaagt aactgctttt
      781 gtttgtgaaa acgatgttgt cgctatccag accatgaatc tcttgagaca gcatggttat
      841 catatccctg aagatttttc aattatcgga tttgatgata tccaagcagc agctcttgtt
      901 gacccgccac tgacaaccat cgcacaagat tttgaacagt taggtaagct tgcgggagat
      961 gctttggtca cttggttaga gacaaaagat cgaccatccg atagtaaatt tccagtttca
     1021 ttcatcaaaa gacaatcaac aaaggagaaa taaaatgaat atcggtcaaa tagatactag
     1081 acatgcaaca gcgaatcagc caaatttctc taatgggaac tgccagcctt atactggcgt
     1141 tccttttggg atgaattatt ttgcaccgca aactacggat caaaacggca gctggtggtt
     1201 tcatcctgat gaccgcgttt ttcaaggcta ccgtcttacc catcaaccaa gtccttggat
     1261 gggtgatttc agtcatatgc tgtttacgcc gatcaatgga aaactacagg agaacaccct
     1321 tttccatgct caaagttctt accgcccaga agaaagtata ttttgcccga ctcatctgtc
     1381 gatctgtcaa ttacgttatg gcatctgttc tacattgata cctagcatgt atggcggaat
     1441 cttatctatt gattacagca aaaatgacag cggtttactt ttatcctttc cgggacgcca
     1501 tcagctcttt gttatagatc cttacacagt ggaaggaaaa atcacacatt ttgctggttc
     1561 agaagatcct gatttttctt tttattttat attgagattt gaatatcctt taacaattga
     1621 tgagttagct gtttccaaca aaaatagcga ctcgatcccg ttgtattttg aacagacaaa
     1681 aaaacaaact atccgatttg ggacatcttt tatcagctcg gaacaagcag cttataacct
     1741 ggaactagaa caaaaccaaa gcaaagagac ttaccttgct cacagtcaag aacaatggca
     1801 gtcctatttt gatcgtatac agctcacaca ccatgatcaa caacaattgc ggacgtttta
     1861 tcataattta tatcgggtgt ttttatttcc acaaacattt tatgaaattg atcctagtgg
     1921 gcaaaaaatc cactatgata cgactagtcg atctataaaa cctggtgttc tctatacaaa
     1981 taatgggttt tgggatacgt ataaaacggt ttatcctctt tattctttga ttgctgtaga
     2041 aaaatacgaa gaaatgctgg aaggtttttt gaacagctat agagaaagtg gctatttgcc
     2101 aaaatggctg tctcctgatg aacgtggact tatgcctggg acattgatcg atgcggtcat
     2161 tgcagatgct gctgtaaaag atatccgacg agacttgatg cctgaatttc taacggcaat
     2221 ggaaaaaggt gctacggttc aaagcaaaca tccaaattat ggacgccaag gaacagaaga
     2281 ttacttgaca tatggctatg ttcctaatca ctaccacgaa tcagtcaatc acacattaga
     2341 ctatgcttat agcgattttt gtattagcca agttgccaag gtactagaag aagaggaaat
     2401 gacgaagaaa tatcagcagc aatcattgaa ctatcaaaat atctttgact atgagtcagg
     2461 atttatgcgt tcaaaagata aagatggcaa gttcagagaa ccattttcag ctaatcgttg
     2521 gggacaagat tatgcagaag gaagtgcttg gcaaagcagc tttgctgttt atcacgattt
     2581 ttcaggactg atcggaaaat atggtggcag tgagagatat aaagaaaaac tcattgagct
     2641 gtgcaatcag cccccaacgt ttgacccagg aggatatggt tttgagatcc acgaaatgag
     2701 tgagatggcc gcgatagaat ttggacaatt agctatttca aaccagccaa gttttcacta
     2761 tccttacttg ttcagttata taggacaacc tgaaatggca cagccactgc ttaaacagtt
     2821 gatgactcag accttcgacg attctccaat gggttaccct ggagatgagg acaatggaag
     2881 tatggccgct tggtatatct tcaatagtct gggattttat cctgtcacgc ctggaaccgg
     2941 tgaatatgtt atcggtatgc cattaattga caaggctgtt ttgcttttgt caaatggaaa
     3001 ggaattgacg attgaagcaa cgcctaatcg gccgcagcaa caatttatcc ataaaatcaa
     3061 acggatggac agagaatata cgaaattata ttttacccat caagatttgc tgaaaggcgg
     3121 cacgatctct taccaattag ggatcatccc tcaccctaag aaatatcaag caaaagattt
     3181 gcctttttcc ttatcaaact ataattaaca agaggttgtg aaaaagctgt cctgcatcaa
     3241 gtaataagaa cagccaagca aaaatagatc ctcatatttt tcgtttggtt ggtcttatta
     3301 ccgcagatgc aagcttttga acaccgttta ttgagaggtt gtgaaatcag cgttctgacc
     3361 tgagtattag agagacaaat ggcaaaatag ttcctcatat tttgccattt tttgagctaa
     3421 taccgagggt cagctgattg aacaccgttt agtggagagg tcgtgaaaaa gctgtttagt
     3481 ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct
     3541 taataccgag aaactggctt ttgaactccg tttatcgaga ggtcatgaaa aagctgcctt
     3601 gcctcaagta ataagaacag cccaaaatta gatttctaga caatttttta taagttattt
     3661 cattacattg gctttttctt ttatgattct ccactgtttt ctcggtactc tttaggggat
     3721 ctgccgacga ttttcttgaa taatttagag aaatacgtgg tgttgttgta gccaatctca
     3781 cttgctatct cattgatgtt ttgattactg tgcaaaagca gttgtttcgc tttttccata
     3841 cgtaaatggt tcaaataagc tgaaaaactt tttttcgttt cttttttgaa cagctgtcct
     3901 agatacatga catttaaatg caggttttct gacacttctt tgagtgtaag tggttcttga
     3961 taatgctggt gcaggattac gattactttt tccacattgt cactgtactt tttctgtctc
     4021 tgatgctttt tgatgaatga cagaagtagc tgctgaagtt cttgcaccgt ttttgcctga
     4081 tggatttttt cgattccttg taaatattcc tcatcttcca gatcaatcaa ttcccgttga
     4141 atctccataa acagtaaaaa actggaatat cgtatatctt ccggcatcat ggctgctaac
     4201 tgaaactgtt caaaaaaatc agtgatcatt ttttgagcga cttccaattg tttattttgc
     4261 agcacgcgtc tgaaacttga aaaatcgatg gtttgatctt ttgtttctgc ttgtactgcg
     4321 taaaaaacat gctcgtcttt atctccataa aattgatgca actgtagatt gtctttcgct
     4381 tgttggaatg tttcagggat tttgtccaaa gaatctgtct cttccccgat actgatcagc
     4441 cattcctgat caggaaagtt ttcttgaaca aaacaacaga agccatcaac ttcatctgcc
     4501 tccaacaaca acgtgatcaa tacacggtct ccgtaatttc gttgatagta aaagatttgt
     4561 tgccgttctt gcaaccatct attgataaga gtatcatgag ttcttgccag ttgaatcaat
     4621 actatcctat gctgcttctc acttatttgt cctattattt cttcttcact tatttcatcc
     4681 aattcttcat tcaaccattg actgaataac agttcttgat agatttcttg ttgatttttt
     4741 tgttcatttt gctgatctaa ccgtgttttg attttcttga ggctttcaac caattcgaac
     4801 ttattcacgg gtttcatcaa ataattcacg gcgcctaatt gcatacctcc tttcaaataa
     4861 tcgaattctt ggtatcctga taaaatcata aattcaaaat tatgacgttc tttttgagcc
     4921 gcttcaataa attccagtcc atttatttct ggcattgtca catccgttac tactaaatca
     4981 atctgtcgct gttcaagtac agctagtcct tctttggcat tctctgctgt tgctactatt
     5041 tgaaagccta aactttgcca atcaattatc ttttttaacc cattcaaaat catgtattca
     5101 tcatctacca ataacacttg atacacgctc atccctcctt gaatgtaata gaaacggtca
     5161 gtccacctgt ctcattattc tcaagcgaca tataataact gctgccgaag ctggcacgta
     5221 aacgttcatt cacgttttgc agaccgatag aaccgtgaag ttccacttta ggatgagaca
     5281 gtttttcttc aatctgtttt agccgttttt cagtcatccc ttttccatta tctttgatga
     5341 tgatccttac ccgtttgcct tcctgcaaga ctttgacact tagcgcatta tcgaatcgag
     5401 taaaatcaat accgtgttta aaataatttt caactaatgg ctggatcaca aatttaggta
     5461 cttcgatctt ttccaaatca ggatcgatca taaaatgata ggctactcga ttaggataac
     5521 gcatttgata caaatagaca tatttttcgc aaaagtccag ttcttctttt aaagtgatcg
     5581 ttttctcttg attcgtattg tttcgaagca aagcggaaaa cgcatacaca acatctgcta
     5641 attcttcact tccttcgctg attgcataca ttcggatata ttctaatgta ttatataaaa
     5701 agtgtggatt gatctgtgct tgaagcgccc gcatatgtgc gtcttgctgt ttgatttcta
     5761 atttgtagat gtcttctaca aactgattga tgctatccaa catttcgttg ataccgattg
     5821 aaagttccct cagttcatat tccgtgtttg ttatgtcgat ccgcgtctct aaattccctg
     5881 ctgctgtttg atccatggcc tgcatgacga tagatacttg ctgtgaatat cgtttgaatg
     5941 ttttataaag aaagataagc agtatattgt ctaaaataat gataccaatg atcagcgggg
     6001 caagattttt tcccgtttcg atttttacac tttttttatc tagtacagct aatatacgat
     6061 aatcatcaga cagttcttgg tgttgaacaa aattgttttt ttccagatcg tcaatcggca
     6121 attgtgaatt tttcttcaga ctctctgtga tgatcatttc ttgtctttca aacgtttctt
     6181 tagctatttt attatcatgg aatgtgtata gtcgatttgt cgttccagaa atcatgtata
     6241 aagagagtcc attgaatgac gttagatttt ctaaaatcat atctagttct ttcttttgaa
     6301 aaccaataaa aatacttcct gactcagctc ctccttgtgt gataggcaac ttgatataaa
     6361 aggcgtctag taattttggt gtcccctttt ttatttcccc acctttgttt tctctagaag
     6421 attcataata ctccggtagt tgattcaaca cgatatatat cgcacttagt tcttcataat
     6481 ttgcgtagag atctttgatt tgatttggga aagaaactaa ttcatcggta ctgttttgct
     6541 gttcgtatac atacatcaga tactgatcta tgggttttgt taagtaggtc gtcacatttt
     6601 cgatcttttc ggaattccct acaagctggt ttttgatgat cctactcaag gttgttttat
     6661 cattcagcat tctgcttgtg ctttgaacgg catcacttgt catgttttct gcctgttttt
     6721 gattttgttc ccaagtatgg atactaaaaa taacggtagc agtcatcgtt acaccgatca
     6781 tgataaccgc gtatttccgc aacagacgat ttagtaaggt tccttttgaa aatagaatcc
     6841 gaaacttatt cataactttc caactgttta tctaattctt cacgccaatg tgttgtcatc
     6901 gtaccattcc aaaccgtcaa aagaccaatg acaccaaaca ggatcaatcc tttgaattgc
     6961 caagtcacgc ctaaaattat accgctacca ataattattt ttagaaaaga cgaaaagctg
     7021 gaaaaaaccg aaataaaact tagctttagc agattaggaa cagagatcgt atattcacta
     7081 tctaaaatca tactgtattg ataagtagca aataggcaga gcaaggcaaa aagtaaaata
     7141 aaatcaatag ctaaaaaaat gagccctttg atttgaa
//
LOCUS       DACDSY010000138         7082 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDSY010000138 DACDSY010000000
VERSION     DACDSY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.140"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            259..732
                     /locus_tag="IWK44_002397"
     CDS             259..732
                     /locus_tag="IWK44_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP9550885.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            729..2861
                     /gene="feoB"
                     /locus_tag="IWK44_002398"
     CDS             729..2861
                     /gene="feoB"
                     /locus_tag="IWK44_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP9550886.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2863..2997
                     /locus_tag="IWK44_002399"
     CDS             2863..2997
                     /locus_tag="IWK44_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP9550887.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3091..3735
                     /locus_tag="IWK44_002400"
     CDS             3091..3735
                     /locus_tag="IWK44_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9550888.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3922..5115
                     /locus_tag="IWK44_002401"
     CDS             3922..5115
                     /locus_tag="IWK44_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9550889.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            5108..6835
                     /locus_tag="IWK44_002402"
     CDS             5108..6835
                     /locus_tag="IWK44_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9550890.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         2295 a         1240 c         1575 g         1972 t
ORIGIN      
        1 aaaacaagta tcgtgtggga aaatagcaaa tttaatagag aaaaaattgt ggaaaactat
       61 gaagaaatat ttttttctca tttttatcta tcaatactaa agattcgctc ataatatcac
      121 aagtataatt gacattctca attaccgaga gtattctatg tgagaatgat tctcatttat
      181 ttgcgtaaca cgtaagaaaa ttagaacata taatgaatga ttttaataca tgatttattt
      241 cacaagagag gaatcaatat gcagacctta gcgcaggcaa agcagaattt cgtttatcaa
      301 gtcgaacgaa tcgaaacaga agaaaaaaca aaacgccatt tgcaaaatat cgggatcgtt
      361 gccggaagta aaatcgtcgt cgtcaattta tcaggcaaca atgccatttt actggtaaaa
      421 agtcatcgag tggcagtaag tcgggaaata ctaggtcaaa tctttgtcaa taaaaagact
      481 gcttcctctg aaagctggac ttctcttgat caattaaaag caggagagac tgggatcgtc
      541 gtagcaatcc acggacaagg cgcagtgaag cgacggttaa tggatatggg attgacgcga
      601 aatgtaaaaa tagttatccg aaaactagcc cctctgggag atccaatcga actgacagtg
      661 cgagggtatg aactgacgtt acgaaagtca gaggccgaac taatcttgat acaaagggag
      721 gaaaaagaat gaatatcgcg ttagtcggaa atccaaacag cgggaaaact agcttgttca
      781 atctcttgac tggttcgaat caatatgtag ggaattggcc gggcgtaaca gtagaaaaaa
      841 aagaaggatc gttgaaaaaa aatccgaaca ttcatattca ggacctaccc ggcatctatt
      901 ctttgtctcc ttatacacca gaagaagtga ttgcaagaga ttatttggtt caggaacagc
      961 ctgaactgat catcaacatt attgatgcta ccaatttaga acgaaatctt tatttaacta
     1021 cgcagttatt ggatttagac attcctatga tcattggtct gaatatgatg gatgtcatca
     1081 aaaaagaagg aaaaaaaatc aatctagaaa agctttccta tggtcttggt gtaccagttt
     1141 taccaatcag cgtattgaaa aagcaaggaa tcgaaaaact aatcgaaaag attcaggagt
     1201 ctgcaaaaaa gcggccttct attcctgttc cctgtacata tgatccacgt ttagaagcag
     1261 ctttacatga aattgaagat gtactaggag aacaaagcgg caagaaacgc tggtatgcaa
     1321 tgaaatattt tgaacgagat cagaaggtaa gagaggatta cgagatagca gtttctcagc
     1381 aaaaagagat cgagcaactg atccagttaa cagagaaact attggacgac gatagtgaga
     1441 cgattctggt caatgaacgt tatgaattta ttacccaact atgtacactc tgtgttgtgt
     1501 caaatgattc tttccaatta tcgatgagtg acaaaatcga tcagattgca acgaatcgtt
     1561 ggttagcctt gccgatcttc gcatttgtga tgtggctgat ctattatctg gctattcaaa
     1621 cagtaggaac aatggggaca gattggatca atgacacatt gtttggtaca tggttaccag
     1681 aacatgttgg cagattgtta gctgaatggc aagtagcggg atggatgcag gagctgatct
     1741 tgaacggcat catcgcaggc gtgggagcag tgttaggatt tctaccgcaa ttgattatat
     1801 tgtttttatg cttgagcttt ctggaagact gcggatatat ggcgaggatc gcttttgtca
     1861 tggatcgact tttcaggaaa ttcggtcttt caggaaaatc cttcattcct atgctgattg
     1921 ctacaggatg tggagtgcca ggcgtcatgg ccagtcgcac gattgaaaac gagcaagatc
     1981 gtcgtttgac gatcatggtc acaaccttca tgccttgttc tgcaaagttg ccgattattg
     2041 ccttagtagc aggggccttt tttcctcatc aatcttgggt agctccttct gcctattttc
     2101 ttggtatgac tgcaattatt ttctctggaa tcagcttgaa aaaaacacgt ttgtttgctg
     2161 gagatacagc tccttttatc atggaattgc ccgcatatca ttttcctcaa tggtcagcgg
     2221 tcctaagaca aacctatgac cgcagcaagt cttttgttaa aaaagcagga acgattatct
     2281 ttgtttctag tatcgtgatc tggttcagct cttcttataa tttttatgct caacctgttt
     2341 ctgaagacca aagtatctta gcctttttcg ggcgaatcct ggcagttgtt tttcagccgc
     2401 ttggttgggg aaactggcaa ggaacagtcg cagcgattac aggattggtc gctaaagaaa
     2461 atattattgg cacatttggt attttgtttg gtcatgcgga agtatccgaa aatggcagag
     2521 aaatctggca agtgctacaa cacacatata ctcccgcagc agcttattca ttgttagcct
     2581 tcaatttact ttgtgcgcct tgttttgcgg caattggggc tatccatcgg gaaatgaatg
     2641 ccaagaaatg gacttggatc gccataggct atcaatgcgg tttagcttat ttagttagtt
     2701 ttgtgattta tcagttggga cacttattgg agggtggaca ggtagcgtta gggacgtatc
     2761 ttgctatttt actgatgata ggactcgttt atgttttgtt ccgtcagcca aaatccaaga
     2821 accagtttta tactatattg tcattagaag gagaggaata ggatggcgac attcttatta
     2881 agtgtgttaa tctttggaag tgcaggcgca attgtctata ctcgtttaaa atccggtaag
     2941 aattgtgatg actgtcaaac agcttgccca gtaaaaaagg aacagatcaa gcgatgatca
     3001 gtcgttgcaa gaggctattc tccccttttt tgcttatttt gctagagcat agtaagatga
     3061 gggaaagtaa aaaaagaaag aggaaagaaa atgtctggaa agaaaaaaat cgtccgcccg
     3121 cgctaccagc agattgctgt tgatttagcc gaacgtatcg ttgaaaaccg atataaagtg
     3181 ggtgagaaga tccatgcccg ctcaacactt gccagtacgt acaatgcttc tcctgagact
     3241 acccgcaagg ctatcaatgt gctggtagat cttggaatca tggaagtacg gcatggaagt
     3301 ggggcatttg ttgcttcgaa ggaaaaagca aaagatttcc tattgcaata tcaagatgta
     3361 caatccatcc aagatacaaa agcagaaatc atggcaagtg tggagaagca acaagatgag
     3421 ctgaatcatt tttctcaatt attggatcag ctggtcaatc aaacgaagcg aatacataag
     3481 atgaatcctt tacttccgtt tgaattatct ttatcagaaa aagcaatgca tctggaccgt
     3541 tcaattagcg agttgaatat ctggcaggcg actggcgcca cagttatcgc aatttctcat
     3601 aatgaagaac tgcttgtttc acctggcccc tatgctaaat ttacggcagg ggacatcgtt
     3661 cattttattg gaaatgaata tacactacag cgtatgacaa acttttttta cccggagcat
     3721 cagcttgcag aatgattgtg tgaaatatta aaaaaatatt aacttggcta aaccttttat
     3781 ttacaaagat ttagccaagt ttttttcagt ttggacaagc aaatttcttt catttttgac
     3841 aaagtgacta catgatgtta acatagtgtg gtcacttttt tcagaaagaa aaatgaattt
     3901 acataaaaaa ggagaatttg tttggtaaaa gtagaagtaa aacatttaac aaagatattt
     3961 ggtaaaaaga cacaagccgc tttagatatg atgaatgatc accagccgaa aacggagatt
     4021 ttaaagaaaa ccggtgcaac ggttggtgtg tatgacgtga attttgacgt aaaagaagga
     4081 gaaatcttcg tgatcatggg gctttccggg agtgggaaat ccacattgat ccgtctattg
     4141 aatcgtctga ttgagccaac ttcgggcagt atctatattg atggagaaga tgtatcgaag
     4201 ttaagcaaag aagaattgcg agaagtccgt cgtcataaaa tcaatatggt tttccaaaac
     4261 ttcggtctgt ttccacatcg aacgatcttg gaaaatacag aatacgggtt agaagtccgt
     4321 ggtgtaccta aagaagaacg acaagaaaaa gcagaaaaag cattagaaaa ttctagtttg
     4381 ctttcattta aagaccaata tcctagtcag ttatctggtg ggatgcaaca acgggttggc
     4441 ctagccagag ctttagcaaa tgatcctgag atcttgctga tggacgaagc gttctcggct
     4501 cttgatccgc tgatccggcg agaaatgcaa gacgaattac tggacctgca agccaatgtt
     4561 caaaaaacga ttattttcat tacccatgat ttgaatgaag ctttacggat tggggacaga
     4621 atcgccttga tgaaagacgg cgaaatcatg cagatcggaa caggtgaaga aatattgacc
     4681 aatccagcaa atgattatgt acgtgaattt gtggaagaag tcgatcgttc caaagtgctt
     4741 acggctcaaa acatcatggt acctgcattg acgacgaata tcgaatctga tggaccaaac
     4801 gttgcattga caaggatgcg taatgaagaa gtcagcatgc tgatggctgt tgacagaaag
     4861 agacacctga aagggatcat cactgcagat caagcgttag aagctaggaa gcaaaaacgt
     4921 ccattgatcg acttcctaga tgaaaatgtg acggtgattg ggaaagacat ggtggtaagt
     4981 gatattttca atattattta tgactcaccg actccacttg cagttgtaga ggatggaaaa
     5041 ttaaaaggtg tcgtcatccg aggtagtgtg attgaagcat tagcagaaac aagcgaggtg
     5101 agtgaacatg aataatctat tgaactatca attaccagtg gcaaactggg tagaaaatat
     5161 cacagaatgg tttacgacaa ctttttctgg cctattttca tttttacaaa cgattggaca
     5221 ggcagtgatg tcggggatta caaatttact gttagtgatt ccagctcctt tgtttatctt
     5281 gttattgacg gtagcagctt tctttatttc taagaaacgt ccaggactta cgttgttcac
     5341 attaatcggt ctatggttta tttataacca aggtctatgg aatgatttga tgaatactgt
     5401 cacacttgtt cttttatcta gtgtgatctc gatcatcatt ggggtaccat tagggatctt
     5461 gatggcaaaa agcagtaaag cccaaagtat tatcaaaccg atccttgact ttatgcagac
     5521 aatgccagga ttcgtttatt tgattccagc agttgcattt ttcggtatcg ggatggttcc
     5581 aggggtattt gcctcagtca tctttgctct gccaccaacc gttcgtttta cgaatttagg
     5641 gattcgacaa gttcctaaag aattagtaga agcttctgat tcattcggga gcacaagtcg
     5701 tcaaaaacta ttcaaactag aattgccttt agctaaaagt acgatcatgg cagggatcaa
     5761 tcaaacaaca atgctgtcac tttcaatggt cgttattgcc tctatgatcg gtgcacccgg
     5821 cttaggacgc ggcgtattat cagcattgca aagagcacaa gtcgggaatg gatttgtcaa
     5881 cggggttgca ttagttatct tagcaatcat cattgaccgt tttacacagc atttgaacca
     5941 gccaaacaat aaaaaagctg caggagcaac aacacagaaa agtaaaaaac gtcaaggact
     6001 gatcattggt gctgtagtag tagtgatctt aggtgcgatt ggtataggga gtttttcatc
     6061 tgccaaagaa acaaaacgta tcaacttatc ttatgttgaa tgggatacag aagttgcttc
     6121 aacaaatgta gtcggtgaag tattgaaaca aatgggctat gatgtaacga tgacgccatt
     6181 agataattca atcatgtgga aatctgtttc aaatggcgag tcagatgcca tggtttcagc
     6241 ttggctacct aaaactcatg gttcacaata tgcacaatat aaagatcaag tcgaagacct
     6301 aggtgccaat ttgacaggag caaaagttgg tttagcagtg ccagcctaca tggatgtaaa
     6361 ttcgatcgat gagttaaccg atcaggcagg taagaaaatc ataggaatcg agccaggagc
     6421 tggtgtagtt acggctgctg aaaacacgat ccaaaaatat gacaacttga aagactggaa
     6481 agtagagaca tcttcttcag gagcaatgac cgttgcacta ggtcaagcga tcaaaaaaca
     6541 cgaaccaatt gtagttacag gatggacacc tcattggatg tttgccaaat atgatctgaa
     6601 atacttagaa gatcctgaaa acggaatggg aagcgaagag cagatccata cgatggttcg
     6661 aaaaggattg aaagaagacc agccagaagc ttacaaagta ttagacaatt tccattggtc
     6721 agaaaaagat atggaaaaag ttatgttgga aatcaacaat ggaaaagatc cacagcaagc
     6781 agcaaaagac tggatcaaag agaaccaaga attagtcgaa agctggaaaa aataaaaaga
     6841 cagacacgaa ataaatggag cttccaaaat tggatcaaat atccaaattt ggaagctcca
     6901 tgttcgtctc tatctttttt gtctataaaa aaagagaatg cgtagctcat cttcgaaacg
     6961 tccaaagacg ccgacaaact attatgaatt tatgggtgcg agtcaaagag atcgatcgta
     7021 ggtatgaaag actaatgtga ttaaagttta ttatgtacga agtgcattct caaaaaatat
     7081 ta
//
LOCUS       DACDSY010000139         6811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDSY010000139 DACDSY010000000
VERSION     DACDSY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.141"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..400)
                     /locus_tag="IWK44_002403"
     CDS             complement(<1..400)
                     /locus_tag="IWK44_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9550891.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFE"
     gene            complement(413..1738)
                     /locus_tag="IWK44_002404"
     CDS             complement(413..1738)
                     /locus_tag="IWK44_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP9550892.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1755..3167)
                     /locus_tag="IWK44_002405"
     CDS             complement(1755..3167)
                     /locus_tag="IWK44_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9550893.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3184..3948)
                     /locus_tag="IWK44_002406"
     CDS             complement(3184..3948)
                     /locus_tag="IWK44_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP9550894.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4052..4927)
                     /locus_tag="IWK44_002407"
     CDS             complement(4052..4927)
                     /locus_tag="IWK44_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9550895.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5182..5266)
                     /locus_tag="IWK44_002408"
     tRNA            complement(5182..5266)
                     /locus_tag="IWK44_002408"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5230..5232),aa:Leu,seq:caa)
     gene            complement(5284..5355)
                     /locus_tag="IWK44_002409"
     tRNA            complement(5284..5355)
                     /locus_tag="IWK44_002409"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5321..5323),aa:Gln,seq:ttg)
     gene            complement(5365..5437)
                     /locus_tag="IWK44_002410"
     tRNA            complement(5365..5437)
                     /locus_tag="IWK44_002410"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5402..5404),aa:His,seq:gtg)
     gene            complement(5499..5583)
                     /locus_tag="IWK44_002411"
     tRNA            complement(5499..5583)
                     /locus_tag="IWK44_002411"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5547..5549),aa:Leu,seq:caa)
     gene            complement(5604..5674)
                     /locus_tag="IWK44_002412"
     tRNA            complement(5604..5674)
                     /locus_tag="IWK44_002412"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5640..5642),aa:Cys,seq:gca)
     gene            complement(5720..5791)
                     /locus_tag="IWK44_002413"
     tRNA            complement(5720..5791)
                     /locus_tag="IWK44_002413"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5757..5759),aa:Gln,seq:ttg)
     gene            complement(5803..5875)
                     /locus_tag="IWK44_002414"
     tRNA            complement(5803..5875)
                     /locus_tag="IWK44_002414"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5840..5842),aa:His,seq:gtg)
     gene            complement(5878..5951)
                     /locus_tag="IWK44_002415"
     tRNA            complement(5878..5951)
                     /locus_tag="IWK44_002415"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5917..5919),aa:Trp,seq:cca)
     gene            complement(5958..6038)
                     /locus_tag="IWK44_002416"
     tRNA            complement(5958..6038)
                     /locus_tag="IWK44_002416"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6002..6004),aa:Tyr,seq:gta)
     gene            complement(6040..6115)
                     /locus_tag="IWK44_002417"
     tRNA            complement(6040..6115)
                     /locus_tag="IWK44_002417"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6080..6082),aa:Phe,seq:gaa)
     gene            complement(6151..6223)
                     /locus_tag="IWK44_002418"
     tRNA            complement(6151..6223)
                     /locus_tag="IWK44_002418"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6188..6190),aa:Asp,seq:gtc)
     gene            complement(6233..6305)
                     /locus_tag="IWK44_002419"
     tRNA            complement(6233..6305)
                     /locus_tag="IWK44_002419"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6270..6272),aa:Val,seq:tac)
     gene            complement(6321..6392)
                     /locus_tag="IWK44_002420"
     tRNA            complement(6321..6392)
                     /locus_tag="IWK44_002420"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6357..6359),aa:Glu,seq:ttc)
     gene            complement(6407..6496)
                     /locus_tag="IWK44_002421"
     tRNA            complement(6407..6496)
                     /locus_tag="IWK44_002421"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6460..6462),aa:Ser,seq:gga)
     gene            complement(6536..6609)
                     /locus_tag="IWK44_002422"
     tRNA            complement(6536..6609)
                     /locus_tag="IWK44_002422"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6573..6575),aa:Asn,seq:gtt)
     gene            complement(6619..6734)
                     /gene="rrf"
                     /locus_tag="IWK44_002423"
     rRNA            complement(6619..6734)
                     /gene="rrf"
                     /locus_tag="IWK44_002423"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2028 a         1519 c         1135 g         2129 t
ORIGIN      
        1 cttcaaaaat gcctggagaa actacaatga tcaccgtatc tttttgtgct tgctccaact
       61 tcttcaaatc tataatagct aacagtgtcc cttcttttac ctcctggtta ctttcaaccc
      121 gtatctgaaa cggacttcct tcgagttcaa ctgtatctaa acccatatgg atcagtattt
      181 ttctgccaga ttcactttcg atcgttatgg catgtcgggt aggaaatata ttatcgatta
      241 ttcctttaac tggcgcatgg atatatccat catgattcgt tatagcgtat ccaactccta
      301 tcatttttga agaaaagacc tcgtctttta ctgctgttaa tggaataatt tgaccactag
      361 ctacagcttt tagaatgttt cttgttttaa agaaatgcac tttttatcac ctctacttta
      421 atgctggcca atattttttg tttactttga tcatttcatc tagtattttt tttgcgactc
      481 tggcatttgg tacggtttga ttcaatgtaa atgcttgtaa agctttttga taagaacctt
      541 caaagtaagc atctactagt aacttctcta ctgcaacttg tgcctccatc aatcctttat
      601 gaaaatcact gattggtttt cttagtgtaa ttggctccac accagttgca ccaacatatg
      661 ccggtatttc aacaactgca tctgaccgta gattcggaat cgccccttga ttaggaacga
      721 ttagcataaa gcgacgacga tcatcattca acaaggaaat agctatatcg acgatatatt
      781 gtccatgctc cccaaaatta aagttcaata cttcttccac aataccgcgg cgtattttat
      841 cagccatttc tttggtattt ttctctcgtc catccatgac catattagca cgtgtatact
      901 ctttgtcagt atgctcaacg accatatcag gatataagta atactctaag taattattcg
      961 ggatattact tggaaaattt ttcgtgatga ctgacatcat ttgaaaggtt ttttgccaag
     1021 ttttatctcc tatattaaaa tcggcgactt gcatttcttt tgttattagt ttttcaataa
     1081 tctctggcat aatttctcgt tgcaattctt tatcataaat cgatgtatac cagccaaagt
     1141 ggtttaaacc ataatacgta acgatccaat tcttacgatc ataaccgtaa ttcactgcaa
     1201 tcgtttcttc aattgaaata gtcatatcac aggcattaat catttttaca ttcggatatt
     1261 tacgacgaac agcttcagaa acgatcgact ctggatttgt ataattcaaa atccatgctt
     1321 tgggggcata gtcttgtata tgatcgacaa tttccaataa acctttgatt gagcgcatcc
     1381 cataagcaaa tcctccgagt ccacacgttt cttgtcctac taatccatgt ttaagtggaa
     1441 tcttttcgtc cttttctcgc atctccaatc ctccaacacg aatttgtgag aaaacaaaat
     1501 cgcatcctgt aaaagcaagc tttggatcat ccgttgcacg aatgcgaata tttgaaaaac
     1561 cttttctttt cagcataaat ttaacaatca agtacatatc catatttctt tcttcatcaa
     1621 tatcatataa acgtatttca gataatggca accgtttttc attgttcaaa atggcttgaa
     1681 cgattcctgg tgtatacgta ctcccgcctc cagctatagt gataatctgt tttttcatca
     1741 tcatcttttc ctttctattc ttctaataat ttattgaatt ctgccataac ataagtcact
     1801 tctaagccga tgataatttg ataatttttt tcagttggtt taatgatccc gcttatccct
     1861 aattgcttga ttcgctgttc ctcgaccatg gtgggatcca ctacctctac ccgtaggcgt
     1921 gtattacaac tatagacatc caggatattg gcttttcctc caaacgcttg tagcattttt
     1981 gttgccatat attcataatt tttacttgct aaccctaact ctgtttcttc taaggttact
     2041 tcattgccaa actctgcctt agcttcacgt cctaatgtct gaatgtttct cttattgatc
     2101 aaataataaa aagtaacgta atacaaggca aaaaagaaaa ttccgattgg aagaatcaaa
     2161 atagcattgg tagcgattcg aaaattgatg agataatcaa caatacatgc tccgaatgta
     2221 aaaccaattc gtatattgaa taagtaacag acaatccctg ctaaaccggc aaaaagtgca
     2281 tggatcacgt acaacatagg agaaagaaac atgaaactaa attcaacagg ctcagtgata
     2341 tttgcaataa atgaagttcc tgcaccgctt cccatgattc ctttgaccat ctctttattt
     2401 tctttataag ctgctttata gatagctaga catatagctg gtataccaaa catcattact
     2461 acaaaaaaaa ctgataaaaa cagtccagcc tgaggatctc cgttgataaa gcgtgtcatc
     2521 tctcctctga aaacttcctt gctaggagat gtatagcttc ctaaatcgta ataaatatac
     2581 gtattcaaca catggtgcag tccaaagggg atcagcaaac gattcaaaaa cataaatatc
     2641 cccacaccta atgctcccat atcaacgagc agttttgcaa aattatcaat cccaatttgg
     2701 atgagcggcc agataaagcc caaaaaaaca gctgcgattg tttgtagaat catcaccata
     2761 gtcaatggga atttttcgcc cgcaaaataa gaaaaaacta ctggtaattt ttgttcttta
     2821 aagcgattat acgtccatgc cgctactaaa ccggaaataa ttccaccaaa aatccccata
     2881 ttcacttctg ggtccataat ttctaagcct tttttcaagg tagcaatcgc taaaaaacca
     2941 gtaagtgcag gtattccttt gtccttattt ttgacaaaac cgattgcaac tcctatagca
     3001 aataagatat ccaagtttcc aaaaacggca tttccagctg caccaattac tgggatgttc
     3061 agcatatcgt cagctgataa acgcatgatc aatcccgcta atggcatcgc tgcaactgga
     3121 agtagcatcg ctcttccaag cttctgcaca tttgatttaa cattcatttt tattcctcct
     3181 ctattaaata tcctcatatg ttactaactc aatcaaagga tcatttttca ctttttgagc
     3241 aaatgctgaa tgaattaaaa attcgacttg gtgggtacga ggatacacaa aacttgaacc
     3301 atttaacaat tcctgatcca aatacgctgc gtgacacatg acttcgatcg ttttatcttc
     3361 tctcaatatc atgagtaaac ttgccaaata ttcttccaca ctcgtattga tttgtcgttt
     3421 tttttcatca gtttctccta cgacatcgaa acaaggctca aaataatcga catgacgaac
     3481 ttcatctttt cgttcaaagt tgctccgaac cggcaaatca tatttttttg ctaatgtaac
     3541 aactacctcc tgattttcat ccaaagtatg agtatgatga tgactgtcta aatgagttgg
     3601 tatgatcccc gcatgataaa ttttttgaat ctgagcattc cattcctgat acagttgatc
     3661 tttgtcaatg ttaaatggat gctgataata agataaggat ctgaactctc cgctttcgat
     3721 aagagtatcg acagttttga ggagtggttt gccacacgtc aatgttaaat ggactcctac
     3781 acctagccta ggcatttcct ttcttagttt cacagcatgc tcgaaacccg gcatatttgc
     3841 catcaatgta gttgatgtca aaattcctcg ctgataagca tccataatcc cataattcac
     3901 accatggcta tagccaaaat catccgaatt aaaaatcact tttcccattt cattctcctt
     3961 cttttttcta ctaaaatagg cgcaaccaaa taatcatagt ccatttacta tgtgtcattt
     4021 ggttgcgcct atagatagtt acgcccttat tttaatagat tcttgctaga tgaattgcca
     4081 aatagatgat ttcattttct tgaatggcaa gttgaaattg ctgttcgatg atatctttaa
     4141 tctttagtgc tgtacaatac gctttgctat gtttaaatgt ttcagtaaaa aaaccaatat
     4201 tttctactgg cgtttcttcc tgataaatca agcgtttaac caggaatttg atatgaacca
     4261 ttaagcgatg ataagtcagt gattctttat cataaacgtt tgaataggat ttttctagat
     4321 gggacaaaat cgtggtcgtc atttctacta tttttaagct ctttaaacta ttaggctctc
     4381 ctagctcagc attaatgata tgcatagtga tgaatcctac ttcatttgac cctaactcgg
     4441 tttgatatcg atgattgatt cgctcgacca cttctactgc tatatcatat tcatttggat
     4501 aaaggaatct catttctggc aacataattg ctgtgacatc tctatcttct ctttttcgtt
     4561 gaatagcaaa ataaatatga tctgtcaatg taatatagat atttgaatct aatggagagg
     4621 agatcctagc tgttatcagt tcaatcactt cttccgcaaa atccataaat tcgattggaa
     4681 tctgcgagac tatctcactg aatcgtttga tcttttcttt agaatctaat acaaataatt
     4741 tgtcagcatc ctcaacatca aatggcttac tcactttagc agaaaagcca atccctttcc
     4801 ccatcgcaat tttttctatt ccatcgtcat cgatcaatac agcattatga ttcagctttt
     4861 tcatcacata caccgttaat tcacctcctc tatatgataa aacaaaacaa cttgcagact
     4921 ccttcataca tatttttgtc tccaaacaat ttttaaagac aaaaaacgac ctaagaaaga
     4981 accctttctt aggtcgtgcc taattgaata gtagcaccct acataattta caaaaatagt
     5041 ttaacacata ataaaagcgt tatcaatatc ttttttactc gttttatgat aatacttgta
     5101 tcgaattgat ttggaggtaa ttacataaaa acaaaaaaaa tatagcaact ctctataaga
     5161 aagttgctat attgataatt ataccggcgg ccggggtcga accggcacgt cctcaacgga
     5221 cactggattt tgagtccagc gcgtctgcca attccgccac gccggcaaat ttaaaaataa
     5281 taactggggt agctggattc gaaccaacgc atgagggagt caaagtccct tgccttaccg
     5341 cttggctata ccccaagaat aaagggcgaa tgatgggaat cgaacccacg aatgccggag
     5401 ccacaatccg gtgcgttaac cacttcgcca cattcgccat cataaaagat gttatttaat
     5461 ttctcaataa aaaactaaga tgaagacatc ttagtcaata ccggcggccg gggtcgaacc
     5521 ggcacgccct caacgggcac tggattttga gtccagcgcg tctgccaatt ccgccacgcc
     5581 ggcaagttga tttattagta ctaaggcggt aaccggattt gaaccggtga tgaaggtttt
     5641 gcagacctct gccttaccac ttggctatac cgccattata ctattatcaa tctataaaat
     5701 aaagaataaa ttatagaaac tggggtagct ggattcgaac caacgcatga gggagtcaaa
     5761 gtcccttgcc ttaccgcttg gctatacccc aagaataaaa agggcgaatg atgggaatcg
     5821 aacccacgaa tgccggagcc acaatccggt gcgttaacca cttcgccaca ttcgccatgg
     5881 caggggcagc aggaattgaa cccacactaa cggttttgga gaccgctgtt ctacctttaa
     5941 actatgcccc tataaaatgg aggagagtgg attcgaacca ctgaacccta aggaacggat
     6001 ttacagtccg tcgcgtttag ccacttcgct actcctccat ggtggcgcgg gacagaatcg
     6061 aactgccgac acacggagct tcaatccgtt gctctaccaa ctgagctacc gcgccatatt
     6121 ttaaaattat taattgaata acaagaacga cggttccgac gggaatcgaa cccgcgatct
     6181 cctgcgtgac aggcaggcat gttaacccct acaccacgga accagttcat tatggaggtt
     6241 aacgggatcg aaccgctgac cctctgcttg taaggcagat gctctcccag ctgagctaaa
     6301 cctccaacat tcaattaaaa tgacccgtac gggattcgaa cccgtgttac cgccgtgaaa
     6361 gggcggtgtc ttaaccgctt gaccaacggg ccaggtaaat ttgctacgga gagtaaggga
     6421 ttcgaaccct tgagacagtt tggcaccatc tacatgattt ccaatcatgc tccttcggcc
     6481 tctcggacaa ctctccagag tgaaagaagc tcaatactct ttctcaaaat gagaactccg
     6541 gcagtaggac tcgaacctac gacatcatga ttaacagtca tgcgctacta ccaactgagc
     6601 tatgccggaa taacatcagc gtggcgacgt cctactctca caaggggcaa cccctcacta
     6661 caatcggcgc taagaagctt aacttctgtg ttcggcatgg ttacaggtgt atccttctcg
     6721 ctatcgccac cacactgtgg tgttatcttt tattgagtaa attttgttca ctcaaaactg
     6781 gatttgaagt cacataagtt tttttccgag t
//
LOCUS       DACDSY010000140         6796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDSY010000140 DACDSY010000000
VERSION     DACDSY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.142"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1060
                     /locus_tag="IWK44_002424"
     CDS             <1..1060
                     /locus_tag="IWK44_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAP9550896.1"
                     /translation="PAIDNMDGIMTVCLGRNMLFDLVIQSYSQLETRYDKAFKTIKEN
                     CQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVERA
                     SQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEID
                     IACEHINLSLQDNQIPYAKFIKDLKTRLEYSIINNIPISDEDYHNYRNLSGSQQTINE
                     EELLKLVNTEEKNQDTEGQVLSEQEMKINIYLKESQKEAIELVKVINGRLADENSADM
                     VIKTINSQVLDTPFIQSLINDSIAIHNAKENKDQIMILEQQLANAYELAVLKTKNSIL
                     KNKIIKLKNAMTKLVKAI"
     gene            1078..3747
                     /locus_tag="IWK44_002425"
     CDS             1078..3747
                     /locus_tag="IWK44_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550897.1"
                     /translation="MTESINWRKLNVRWLKYKWPIIVSLIIILFVSYPQQVFALPWIT
                     DNKEVLNFLTKYQDYLQFTKGGFGSIVMQSLSWGLIRGAYFVCKSAENLVPQSLSLFS
                     FVESSSLGAVYQSVLNTVVVGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSVALIL
                     LMPTMISSGIQFAKTFYKDSTTITKADGGVAWSLIEDGVTDLVYINKNDAYDRLGKDG
                     VQKNALKEKNFFSYDFSEVITVDTAKDLEDDNKKAEYLQSRLTPDGEGNMQAVKIDDG
                     WMSMFSDSFKGGYYRFTKNTFALTLGLLSLAVAYVFSIFIIVTAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFISYLGTGGGSSVNPFVKAVA
                     YVCAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGN
                     GNGSDSREGEDRKPEKNFGETLSKKAGKTGRVLGYAHERGLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKSMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPTSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGVNMDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQSGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDV
                     RENVQSSSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQSSSASQPKTVDVRENVQN
                     STSSQGFKTVDVRENVQKDSGEMKEKTQKINIVEDRKSAKKIDSNHETVIIDSEIREN
                     DKGSKPRRRFTYEDNELFRDTLNDPNPLFDNLN"
     gene            3795..4106
                     /locus_tag="IWK44_002426"
     CDS             3795..4106
                     /locus_tag="IWK44_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550898.1"
                     /translation="MMYRNPKNIKQEIKLFFFYLVDLGIVAGSVMASVYFAKLMPISP
                     IMKFALYLLAFVMGVWMCVRTPNHPKERNILMLYYLFKMDRNRYHEVEFKRNSRGKIE
                     L"
     gene            4103..4720
                     /locus_tag="IWK44_002427"
     CDS             4103..4720
                     /locus_tag="IWK44_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550899.1"
                     /translation="MIVAESNENKLSGVFDKFLELVIPKDKDICELLPYKKILKDGTL
                     VTNDNKYQAFFKVKTTDLTSISEDGFMRMVNKLTYLNRVYLEPYKIYSLTYPTETVNQ
                     AIYWKHRIQEAKRSLLSNNDPNLETYLKWNLKLAYDNYERVDLVSTLPELMFVICVYG
                     KTQKEVKNHADVMKQAGGRELDLQRITEGDLINVITKLMNPNTSL"
     gene            4737..5204
                     /locus_tag="IWK44_002428"
     CDS             4737..5204
                     /locus_tag="IWK44_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550900.1"
                     /translation="MLKHCRKGFCKVIKGKNATDLIFEWKESNLINVRGKNGSGKTER
                     VMDSIDNSRPRSRALVITDEKNHYKGIRTIEKKYLTNNDLKVVDDILNIYPDFNLVVF
                     DETIPVTEIKGFYQSFSNLANKYKEVLFVWITEENSNSKSAFFSEKNAFLHSI"
     gene            5220..>6796
                     /locus_tag="IWK44_002429"
     CDS             5220..>6796
                     /locus_tag="IWK44_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tra protein"
                     /protein_id="HAP9550901.1"
                     /translation="MIVSKKLARNLVNEGYDLRLVEKYQPSGGIKFDERYISTGDGYV
                     TSIAVIEFAEDVNSRWLTDIMAIPNTIATFDVCTADKEDLLHKINTSIGELDYRSANE
                     NRTTDKTDALLDKYNLEDFAQEITKRGEVVKTVHARIFVYSDSLEELEKRISEIRKDL
                     ASVNHKAIVPQFRMKDEWMSLFQDGMEQESKLFSKKGLTVPAKNIGGGIPFHHKSLKD
                     PRGFYLGTTSTGGAFIFDPFYSTKQRRSFNGFILGKMGFGKSTLIKQLQEGLQAKNCF
                     IRGFDKSGEFEGMVKKAGGVYVDLGGGDMVVNPLQIYATVMKKDQFGNDVVDESKSFQ
                     NHITKCSNQIRFLEPEIDTATVREYTLMLQDFYIHYGILPQEWREARDDNERNQLNTN
                     ICSLSNDSYPIYGEFLKFIENQQFKIKNATPERVRSIELIKLIVTDMCTTYPQIFNGV
                     TTMPNFDTTKIVYYNIDSISKMSAPVFHCQLFTALTTIWNSALQNGKIQKENFKKYGS
                     DYYKYAQDFMVLIDECH"
BASE COUNT         2537 a          883 c         1370 g         2006 t
ORIGIN      
        1 gccggctata gacaatatgg acgggattat gacggtatgt ctagggagaa atatgctttt
       61 tgacttagtg attcaatcct atagtcagct agaaacaaga tatgataaag cttttaaaac
      121 aatcaaagaa aactgtcaga atcatatttt aatcatgtcg aacgatgagg aaacgataga
      181 agaaatttcc aaaaaatgtg gtcataagac aacaataaat aggagtgcta gttcaaaaca
      241 tttagatact gatagctcgg taactagttc ggcagaacaa gagcgagtaa ttacagttga
      301 gcgtgctagt caattgattg aaggggagca aattatttta agaaaccttc atagacaaga
      361 tacaaaaaga aataagatta gaccatttcc tatctttaat actaaagaaa cgaatatgcc
      421 gtatagatac caatttctag cagaagattt tgatactcaa agagatatta atgagattga
      481 tattgcatgt gaacatatca atctatcttt acaagataac caaattccat atgcaaagtt
      541 tatcaaagat ttaaaaacac gtttggagta ttcaattatt aataatattc ctattagtga
      601 tgaagactat cataattatc ggaatctatc aggatcacaa caaacaatta atgaagaaga
      661 gctgttaaag ctagtaaata cggaagaaaa aaatcaagat acggaaggtc aagttctatc
      721 agaacaagaa atgaaaatta atatttatct aaaagaaagt caaaaggaag caatagaatt
      781 agtaaaagta attaatggaa ggctggctga tgaaaattcg gcagacatgg ttataaaaac
      841 cattaattcg caagtccttg atacgccctt cattcaatct ttaatcaatg attctatagc
      901 tattcataat gctaaagaga ataaagatca aattatgatc ctagaacaac aattagctaa
      961 tgcttatgag ttagcagtgt taaaaacaaa gaattcaatt ctgaaaaata aaattatcaa
     1021 gttgaaaaat gctatgacta agctagtaaa agcaatttag agagaaggga agaaatattg
     1081 actgaatcta ttaattggag aaaactaaat gtacgatggt taaagtataa atggccaatc
     1141 atagtttccc taataatcat tctctttgtg tcttatccgc aacaagtttt tgcattgcca
     1201 tggatcacag acaataaaga ggtattgaat tttttaacaa aatatcaaga ttatcttcag
     1261 tttactaaag gtggttttgg gagcattgtt atgcaaagtc tatcgtgggg gctgattaga
     1321 ggtgcatatt ttgtttgtaa atcagcagaa aaccttgtac cacaatcgct aagtctgttt
     1381 agctttgtgg aaagtagtag tttaggtgct gtttatcaat ctgtattaaa cacggtggtt
     1441 gtaggattaa tgatcctttc attgatgttt gttggttaca agatgattac tggaaaagga
     1501 acagtggatt taaaatcggt tggaatgaac gtagtgatgt ctgtagcatt aatcctttta
     1561 atgccaacca tgatttctag tggtattcaa ttcgctaaaa cattttataa ggactcgact
     1621 acaattacaa aagctgatgg gggcgttgct tggtcattga tcgaggatgg tgtaactgat
     1681 ttagtatata taaataaaaa tgatgcttat gatcgattgg gtaaagacgg cgtacaaaaa
     1741 aatgcattaa aagaaaagaa tttcttctcc tatgatttca gcgaagtaat cacagttgat
     1801 acagctaaag atttagaaga tgataataaa aaagctgaat atctccaatc taggttgaca
     1861 ccagacggcg aaggaaatat gcaagccgtt aagattgatg atggttggat gtcgatgttc
     1921 tctgatagtt ttaaaggggg gtactacagg tttactaaaa atacttttgc gttgacttta
     1981 ggattacttt cgttagctgt agcttatgtt tttagtattt ttatcatagt cactgctatt
     2041 ttagaactgg catttaagcg tgtgcttggg gttcttgttt ttgcaacgga tattgagaca
     2101 ggtcagcgtt caaaagttgt tctgtcggat attctacagt gctatttgac tgttggtttt
     2161 caaggatttg gattatccat gtttgcaatg tttatttcct atttaggaac cggcggaggg
     2221 tcttcagtta atccgtttgt taaagctgta gcttatgtat gtgctgtttt tgttttaata
     2281 aagggtagtg gaacagtcat gagatacttt ggtgttgata ttggactcaa agaaggctat
     2341 gggcaacttg cttctgcttt tggaatgggt gcaatgttgt ttagaaaagg atcaaatgga
     2401 tttaatcgtg ccaaaggaag cggaaacgga aatgggtcag acagcagaga aggagaagat
     2461 cgaaaaccag aaaagaattt cggcgaaact ttatcaaaga aagctggcaa aactggaaga
     2521 gttttgggat atgctcatga acgtggatta tcaggtttag cttcagatgg agctacaatg
     2581 gctagtgaaa gagcaacgaa accatttaag agtatgcgtg atatggctaa cgataccaag
     2641 aacaagttca aagaaggttt agatgatggt accgtgtccg ctattaataa aaatagcaaa
     2701 tctatgttgg ctaaaaataa agaagatgaa actggaaaat atgctgattc catgccaaca
     2761 cgtctttcgg atagaaaaga tggtgctaag gttgaaaatg ctgatagaat tatgtctagt
     2821 tctgaacgta tgcgtgaagc aatgaaaaat aatgctgaat cgaataataa tcctacaagt
     2881 gcaattcagc aaaaagttca acaagatatt gaagaacgca aaaatgctat gcacggtcaa
     2941 gctaagtctg ctgaagaatt aattaatcag aagcgacaag aagcgaaata cacgcctgaa
     3001 gcaatgaatc gtgaggaaat gttaaggaaa cgtgtagaag ggcgtacagg cgttaatatg
     3061 gacgaaaaag aagcgttgac caaagaaaga atccaagaag ctaaaaattc aaatacagga
     3121 ttagaaaaat tagtcaaaga aaatttacaa aattctacaa gtagtcaaag tggccgatca
     3181 gtagatgtcc gagaaaatat tcaaggttca ctgaatggtc atgtcggacg gaatgttgat
     3241 gtaagagaga atttacaaag ttcttcaaat gctcaaggca ctaagacaat tgatgttcga
     3301 gagaatgtgc aaagtagcag cacaagtcaa ccaaaaacag tagatgttcg agaaaatgta
     3361 caaaattcta caagtagtca agggtttaaa tcagtggatg ttcgagaaaa tgtgcaaagt
     3421 agcagtgcaa gtcaaccaaa aacagtagat gtccgagaaa atgtacaaaa ttctacaagt
     3481 agtcaaggat tcaaaacagt agatgttcgg gagaacgttc aaaaagatag tggagaaatg
     3541 aaagaaaaaa cacaaaaaat caatattgtt gaagacagaa agtcagcgaa aaaaattgat
     3601 tcaaatcatg aaacagtgat tattgatagt gaaatccgtg aaaatgataa gggatcaaaa
     3661 ccaagacgaa gattcactta tgaagacaat gagttattca gagatacact taatgatcct
     3721 aatccacttt tcgataattt aaattaaatt aaaagccggc atgagtgatt gtgccggctt
     3781 tttatgaggt gaaaatgatg tatagaaatc ccaaaaatat aaaacaagag ataaagctat
     3841 ttttcttcta tttagtcgat ttaggaatag tagccggaag cgtaatggca agtgtttact
     3901 ttgcaaaatt aatgccaatt tcaccaataa tgaaatttgc actgtactta ttagcatttg
     3961 taatgggtgt ttggatgtgc gttagaacgc ctaatcaccc taaagagcga aatattttaa
     4021 tgctgtatta tctctttaag atggatagaa acaggtacca tgaagtagag tttaaaagaa
     4081 atagtagggg gaaaattgaa ttatgattgt tgctgaaagt aatgaaaata aattaagtgg
     4141 tgtttttgat aaattcttag aactagttat tccaaaagat aaagatatat gtgaactttt
     4201 accatataaa aaaatattga aagacggaac gttagtgact aatgacaata agtaccaagc
     4261 tttttttaaa gttaaaacaa ctgacttaac ttctatttca gaagatggat ttatgcgtat
     4321 ggtaaataaa ctcacttatt taaatcgagt atatttagag ccatataaaa tatattcatt
     4381 aacttatcct actgaaacag taaatcaagc gatttattgg aaacatagaa tacaggaggc
     4441 aaagagaagt ttgttgagta ataatgaccc caatttagaa acgtatctaa agtggaatct
     4501 aaaactagct tacgataatt atgaaagagt tgatctagta tcaactttac cagagttgat
     4561 gttcgttatt tgtgtttatg gaaaaacaca aaaagaagtg aaaaatcatg ctgatgtcat
     4621 gaaacaggcc ggtggtagag agttagattt acaaagaatt actgaaggtg atttaatcaa
     4681 tgtgataact aaattgatga atcctaatac ttctttataa ggtggtgctt attattatgt
     4741 taaagcactg tagaaaagga ttttgtaaag taattaaagg taaaaatgct actgatttaa
     4801 tctttgaatg gaaagaatct aatttaatta atgtacgtgg gaaaaatgga agtggaaaaa
     4861 cagaaagagt gatggatagt attgataatt caaggcctag atcaagagca ttagtaataa
     4921 ctgatgaaaa gaatcactat aaaggcatac gaacgataga aaaaaaatat ttaactaaca
     4981 atgatctaaa agtagtagat gacattttaa atatttatcc tgattttaat ttagttgttt
     5041 ttgatgaaac aattcctgta acagaaataa aaggatttta tcaaagcttt tccaatctag
     5101 caaataagta taaggaagtt ctgtttgttt ggattacaga agaaaatagt aacagcaagt
     5161 cagcgttctt tagtgaaaag aacgcttttt tacattctat ttaaaaagga gaattgaaca
     5221 tgattgtaag taaaaaatta gcaagaaatc tagtaaatga agggtatgac ttaagactag
     5281 tagaaaagta tcaaccgagc ggtggaatta aatttgatga gcgctatatt tctactggtg
     5341 atggatatgt aacttcgatt gctgttattg agtttgcaga agatgtaaac agtcgatggt
     5401 taacagatat aatggcaatt ccaaacacta ttgcaacttt tgatgtatgt acggcggata
     5461 aagaagactt attgcacaaa atcaacacgt ctattggaga attagattac cgatcagcaa
     5521 acgagaatag aacaactgac aaaacagatg ctttactaga taaatacaat ttagaagatt
     5581 ttgcacaaga aattacgaaa cgtggtgaag tggtcaaaac cgttcatgct cgtatttttg
     5641 tttatagtga ctctttagaa gaattagaga aaagaattag tgagattcgt aaagacttgg
     5701 ctagtgtcaa tcacaaggca attgttccgc aatttagaat gaaggatgaa tggatgtcat
     5761 tatttcaaga tggtatggaa caagaatcaa aattattttc caaaaaaggg ttaactgttc
     5821 cagcaaaaaa tattggtggc ggtattccat ttcaccataa gagtttaaaa gatccacgtg
     5881 gtttttactt aggaactact tcaactggtg gtgcttttat atttgatcca ttttattcta
     5941 cgaagcaaag acgatcgttt aacggcttta ttttaggaaa aatgggtttt ggtaaatcaa
     6001 ctttaattaa acagctacag gaagggctac aagcgaagaa ttgttttatc cgaggatttg
     6061 ataagtcagg cgaatttgaa ggcatggtga agaaagcagg aggggtatac gtcgatttag
     6121 gtggtggtga tatggtagta aatccattac aaatttacgc aacagtcatg aaaaaagatc
     6181 aatttggaaa tgatgtcgta gatgaatcga agagttttca aaatcatatt actaagtgta
     6241 gtaatcaaat tcgcttttta gaaccggaaa ttgatacggc aactgtacgt gaatatactt
     6301 tgatgttaca agacttttat attcattatg ggattttgcc acaagaatgg agagaagcga
     6361 gagacgataa cgaacgaaat caattaaata caaatatctg tagcttgtct aatgattcat
     6421 atccaatcta tggagaattt cttaaattta ttgaaaatca acaattcaaa attaaaaatg
     6481 ctactcctga acgtgttcgt tctattgaat taattaagct tattgttacg gatatgtgta
     6541 caacttatcc tcaaattttc aacggagtga caacgatgcc aaattttgat acaacaaaaa
     6601 ttgtttacta taacattgat tctattagta aaatgagtgc cccagtgttc cattgtcaat
     6661 tattcacagc gttaacgact atttggaata gtgcattgca aaacggaaag attcaaaaag
     6721 aaaactttaa aaagtatggt agtgattatt ataagtatgc acaagatttt atggttttaa
     6781 tcgatgagtg tcacaa
//
LOCUS       DACDSY010000141         6755 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDSY010000141 DACDSY010000000
VERSION     DACDSY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.143"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            271..2946
                     /locus_tag="IWK44_002430"
     CDS             271..2946
                     /locus_tag="IWK44_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP9550902.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3128..3367
                     /locus_tag="IWK44_002431"
     CDS             3128..3367
                     /locus_tag="IWK44_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550903.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3383..4756
                     /locus_tag="IWK44_002432"
     CDS             3383..4756
                     /locus_tag="IWK44_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9550904.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4760..6394
                     /locus_tag="IWK44_002433"
     CDS             4760..6394
                     /locus_tag="IWK44_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9550905.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6557..>6755
                     /locus_tag="IWK44_002434"
     CDS             6557..>6755
                     /locus_tag="IWK44_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550906.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2382 a         1080 c         1349 g         1944 t
ORIGIN      
        1 acttgatttt ataggaacat taggatggac agaaacatac tatgagaaaa tatataaaga
       61 aataattgaa caatattttc ctaaataatt taataaggat tgatttatcg taaaagaaaa
      121 aagttgtgat ttccaaaatc acaacttttt tcttttgtca agattttgag tgggatatat
      181 ttttgtagaa ctgaagatca tgaaattatg agaggttgga ggtaatgtag tcatacaaat
      241 aaaaattttt tagaaagtga gtggtaatta atgaaaatga atcacacttc aattcagaag
      301 catttacaac tgttaaatac actggggatt tcttatgtta atttgagaac tttttctaca
      361 ttaaacaaga aaaccgtttc tcatattttt tctactgcag attctcaagc aattattaac
      421 tatttagaaa aacagaaatt gtctgaaggt acagtaaaca taacttataa tcctattaaa
      481 caagaggtga ttacacaaaa aaagcattct gtgagagaca atgatattga aaaaattcgg
      541 tttgtattta ttgatattga ccctaaaagg aaagagggaa gtgcaacaga tagcgaaaag
      601 aaaaaggcag aaaatgttat ggaacaagta gaacgttatt tgaaagaaaa gagaatagaa
      661 tcatttgtaa aagttgatag tgggaatggc taccatattt ttcttcccat aaatgaacaa
      721 ccaaataatc acgagacgat tcttactatt cagaattttc ttcaattact tcaccgaaga
      781 tttggagtag aagatgaagt tgatattgat acttctgttt ataatccttc aagattatgc
      841 aaattagttg gtacaccagc ggtcaaagga aagtccacaa aagagcgtcc gcatcgttta
      901 tctagtttcc tttctattcc cgataacata gatagaagta attgttttaa aattcttgaa
      961 cagatagttg aagaaaattc catgatgtca acctctagag taaaaagcca tccatctttt
     1021 agagaagtgt taccttttat tcaagcagac tataaacaat ggttagctca ctatcctgaa
     1081 ttaacttata ccataaaaga gggcgatcat aaaggagtta ccttacttat atttgacgaa
     1141 tgtccattaa gaaaacacac gaataattct aatggttctt gtttgtctgt tacggctgat
     1201 aataagattc gatttcattg tctgcatgct tcggataaaa ataaagatat tcaagacttt
     1261 gtaaaaaaat atccgttgcc aaaacctgta aatccttctg tagaatcatt agaatccaat
     1321 caagaatatt attttgacca gtttaaactg aatcaagaag gagtattttt ggtgaaagaa
     1381 ggtcaagaag aaaaagttgc ttgtccgatg tttattaagc aaatacgcaa aaatatcgaa
     1441 caaaaagtca ttacacttgt tcttgcctat tatgtcgatc attgttggga tgaaatagaa
     1501 atagaaggtg gatgtttaca aactaataat tttaaacaat tagcaaaaca tggcttaatc
     1561 attaaagcta gaagagaagc tgaagtcgtt gactttttac agattcaacg ggagaatgct
     1621 ccaatctttt ttgaacatga atctcttggc tggaaaaaag aaaatgaagc aatgattttt
     1681 cagttacaag attcttttgg acgtgaaatt ccttctgtat taactaaaaa ctcatgctac
     1741 caattaacaa ctaatggtac agaagcggaa tttgacgcat tggttcataa gtatgtttta
     1801 ggaacgaact tagaacttgc ttggtcgatt ggtattgctt ctattgtgtt aggctattta
     1861 aacgttacga aggctaatac ttttttgagt ttactaatta acctacaggg caagagcaca
     1921 acgggtaaaa ccactacgtt acactttatt gcctctttat ttggaaatcc aactacggtt
     1981 cttagaaata tgaatgctac caataaagcc attataaagc tagcatcaaa caatcaggga
     2041 ggtgttccac taatactaga tgagttagga gcagcaactc aaaataactt tagctcgttg
     2101 atctatcaat tagcttcagg agaagaaagg ctaagattag attctaattg tcagcttatg
     2161 ccacaagaga ctttttctgt tttgaccctt acctcttctg aagagcgttt ggaaaattat
     2221 ttcaatgaga aattaactgg tttgaaagtt cgatatatcg aattttctga tatcaagttt
     2281 acaaaggatg cgaatcacgc agagaaagtt aaaagtcttt cttctaatac ttttggttgg
     2341 ggtataaagt tatttctttt gaaactcttt gaacaaggga ttacaaaaat tgaagcaact
     2401 tttgtcgaag ctcaaaaaaa actatgggct gttttacctg aacattcttt gaagcaacgc
     2461 attggtaata atctagcttt gatttatagt ggagccctcc taattcaaga tatactgggc
     2521 tatcctttac gattagagca tatacagacg gtgcttatta attcgtacga acagttaatt
     2581 gtagatacta gtttaggaag tgagcaaacg gtatttgacc gagtgaaaac attgttggtg
     2641 atgaatagcc ataaattttt aacaaacagt gacaacaaac atacgcccag tacgatttgg
     2701 ggaaaagtat caattcaaaa aaatggggat attaaggtaa atatttttcc tatcccgttt
     2761 gaggacttgt taaggcgaga atttcaagta aaggatctaa cctatctttt taggcaattg
     2821 cttgattcgg gtaatatgtg tgcagaaaaa gggagaagaa cgaaacgtat tcacatgaat
     2881 cgtcaagttc ttccaacata cgagattcta ttaccttctt cactcaaatc gtattttcga
     2941 ttgtaattta gaataaacca aagtatgaaa gtgaggcacc atgttatgga aatatttctt
     3001 attgatttag caaaacaact gattacttat ctgtatgata gcgagcaaac ccaaacggaa
     3061 gtaaatgaag cttgatctat gcttcattta cttcattcaa tcaaacatga aagaaaggaa
     3121 aattctaatg actaaaaata aaatgacact aaaagcagag gttcttctgt atattcaaga
     3181 acatttttca aatcaagcct tttttacaaa accaatttac ttggatttcg aaataagagg
     3241 ggtatctgct ggatctatcg ggggtactct tcaagcgttg aagaacgaag gatatttaga
     3301 aaatcacttt gtgcaacgtt cgttcaatag aagagtcgtg aaaaaatggt atttagttca
     3361 ttcatagaga ggggaatcaa gcatgcgtgt ggcttgttat gatcgaacgt ctgtttatga
     3421 agaagcgaaa attagtaatg acgctcaata tgatgcagtg aaagagtatg ttttagagaa
     3481 atatgaacaa attccccaac gattttcaga caaaggagaa tcaggggaaa aagcggagcg
     3541 tccagctttt cgacagctta gagcttgggt ttgtgcgtca gaagaagagg gggtgatcta
     3601 tgtttggcgt tatgatcgat tggcacgtca agccaaaata gccttagaat ttattgagtt
     3661 gtgccaacaa catcaggttc aagtgtattc gatttctgaa cctcttcctg cttctttctc
     3721 acacgcgtca acgaataaac tatttgtgca attattgttc acttttgcag aacaacaacg
     3781 aaacgtcatt attgagaatg ttcgtgccgg actttcctat aaaaagcaac aacggcaata
     3841 tttgtcttct gcggtaccat atgggtatcg tttagtgaat gggaaaatcc aacaagaagt
     3901 acacgaagca aggattgttc gctatatttt tcaactttat ttgaccaaaa aatatggcta
     3961 caaaaagctt tgtcaacgat tgacgcaaca aaagcttttc ttccgcgaac gtccttttca
     4021 gccctatcat atttatagta tcctaaaaaa tccattgtac tatggcgaaa tcaaaggagg
     4081 atctttgggg aagtatctag gaacctttga accgattctc tcgaaagcaa cctttttaca
     4141 agcccaagag attcgccaat caaggtgtac agctaaaaaa gatacgtatc tgtacttatt
     4201 acgtcaaaaa attcggtgtc ccttctgtgg tcgtcatctg agttcgaagt atcaatggaa
     4261 tacaaagaag acgaagacgc ttcattatta tcattgtacc gaccgaagct gccaaggaat
     4321 ttttcttcgg gcagaaaggg ttgagtcaga agtattggaa aagatacagg ctttttttca
     4381 atacgaaggc gtctatcaag ccttgattgc ccaattaacg caacaattag ccgaaaagcg
     4441 aaaagatgaa aaacgcaaag taatccaaca aaagaaacaa gaagcacaat ggattaaggc
     4501 atttgaagag ggcaattgtt cactggaaga actcaagcaa caactgcaag ctcttcaaaa
     4561 gccgatgtct acttcttcgt ttctttcact ccaacagtgt gaagcaaaag tgaaagaatg
     4621 gcttcaacta aaagacctct ctagtcgaca attattatgt gactatgttt gtcggattga
     4681 ggtaacaaag gataaaacag tttgtgggat ttacttccga caactgacac aaaatatttt
     4741 agaaaggaac gaatgatcta tggaaatgat acaaaccaaa cgagtcgcat tgtatattcg
     4801 cgtgagtaca caagaacaag ctttagaagg acatagtatt caagcacaag aacaaaatgg
     4861 acgacagttt gccgaacgga tgggctatga agtggtagaa gtttatgccg atgaaggaat
     4921 cagtgggaaa tccacaaaaa atcggttcgc ctatcaacga atgatgaacg atgctcggaa
     4981 ccatcgtttt gatctactag tgatttggaa attgacccgt ctcggacgca atatgttaga
     5041 tgtcttacaa acagtcgagg aattattgcg atatgacgta ggattacatt cgatctcgga
     5101 acaatttgac gtgaccactt cgacaggtaa attgatgtta caactccttg ggagtttcgg
     5161 tgaatttgaa cgcaaccaaa ttgcggagaa tgttcaaatg tcaatgcaaa gcttagtgaa
     5221 agataaaaag cgttatgcag gaggccgacg tttaggctat gtcagtggta ttgacacgaa
     5281 tggtcaaaaa caattggtca ttgaaccaga agaggcgaaa attgttcgtc tgatctatgc
     5341 aaaatattta ggcggagcag ggtatcgagc gattgccaat tttttgaatc gtcaaggata
     5401 tcaaacagtc aaaaagaatg ctttttctac cacggcagtc aaagatatct tacgcaataa
     5461 aatttatgca gggtatttag aatatgcgcg ctacatcaat tgggaaacca aacgacggaa
     5521 ggggaagaat ccgagaccga ttttagtgaa aggaacccat gaaccgatta tttcggaaga
     5581 agattatcaa aaagtacaag aacgccttgc tttagaagga aaacaaccca aatggaacca
     5641 tacaggtgaa aatgtattga caggcttgtt acgttgtcca gaatgtggcg caccgatggc
     5701 agcgagtaat gtcacaaata ccctaaagga tggcacaaag aaacggattc gatattattc
     5761 ttgttcagtc tttcggaata aaggagcaag tgtgtgtcat gccaattcga ttcgtgccga
     5821 acaagcggaa acatttgtcg ccgaccgatt aaaggagatc gttcagctac cagaagttct
     5881 tccccgattg gtggcagcgt taaatgaaga aatagtgcga caaagccaac cattggaaca
     5941 agaactcgtg gtgttactgg aacgaaagga agaattgaag actaaaattg aaaaatggga
     6001 agctgctttg gaagatagtc ctgaattgtt tccgatgtta aaagaccgct tagacgaatt
     6061 gactgaaaaa cgacgacagt tgcacattcg agaaaatgaa atcttaggga tctttcaaca
     6121 aggagagccg attcaagtca aagatgtcca acggatattg actaatttgg atcgttttct
     6181 tgcccagagt gagaagaagc aaatcaaagc cttgtatcgt acctttatag agaaaataac
     6241 ctttgatcct aaccataagg agatgttgga aattacgatg aaattcacgc atgccgtggt
     6301 acaacaattg aatgagcaat atcaaatagc cgtatccaaa acaaaggata cggctatttt
     6361 tgtgttaaaa acacccttta cgttaacgat ctagtctagg ttatatcaaa caacgttaga
     6421 ggggcgctaa cgcttctcta gcgatttgaa atcgttagag aagtagttag ggaggtcctt
     6481 atcataaata gggatattta ttaatgaata tttgaaatat aattgtatat aagaattaaa
     6541 aagagtgggg atagatatga aaaagatggg taagtgtatt ttatgtgaaa aatacactga
     6601 acttactaaa gaacatgtac cacctaaaaa atcagggaat actggaggta aaaagaccag
     6661 aactggtaat ttagatgatt tccttaagag cgattttaca aaaggtgatt ttccgaaggg
     6721 gattaaaagg aaacctcaag gaaatgttta ttata
//
LOCUS       DACDSY010000142         6724 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDSY010000142 DACDSY010000000
VERSION     DACDSY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.144"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            297..1406
                     /locus_tag="IWK44_002435"
     CDS             297..1406
                     /locus_tag="IWK44_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9550907.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            1489..2385
                     /locus_tag="IWK44_002436"
                     /pseudo
     CDS             1489..2385
                     /locus_tag="IWK44_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            2401..3225
                     /locus_tag="IWK44_002437"
     CDS             2401..3225
                     /locus_tag="IWK44_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9550908.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            3257..4567
                     /locus_tag="IWK44_002438"
     CDS             3257..4567
                     /locus_tag="IWK44_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP9550909.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            4715..5974
                     /locus_tag="IWK44_002439"
     CDS             4715..5974
                     /locus_tag="IWK44_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9550910.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            5984..>6724
                     /locus_tag="IWK44_002440"
     CDS             5984..>6724
                     /locus_tag="IWK44_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9550911.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLG"
BASE COUNT         2255 a          998 c         1355 g         2116 t
ORIGIN      
        1 taaaaaagta aacatccaaa aaagggtgtt tacttttttg tttactaata tttttagtaa
       61 aaaatctcga caaaacatta agtaaacagt tatggtgttg ttaacaaaaa gaaaacgcat
      121 tcaaaacagg aggaattaag atgaaacatt tcaaaaaaat agctttaaca gggatagctg
      181 ttttaactgg gacattactc gcagcatgtg gtgggggaga tagtaattct aaagacgggc
      241 aagtaacttt aagctatgcg agttgagata ctgaccaagc tccaggacta agaaaaatgt
      301 tagatgaatt tgaaaaacaa aatcctgata tcaaagtaga aatggagaca acgccttggg
      361 atcaatattg ggttaaatta gaagctgcaa caacgggagg taatatgcca gatgtagtaa
      421 ccatgcactc tagcgaatcc tataaatata tgtcacagaa tgctttgatg ggacttgatg
      481 atgttatcaa agaaaatgat attgacatgg gaaactttac tgaaggaatt gctgatttct
      541 atacgcttga agacgaacta tacgcaatcc ctaaagatgc ttctgtagtg gggctatggt
      601 acaacaaaga attatttgat aatgcaggtg tcgattatcc agatgaaaca tggacatggg
      661 aaacactcag acaagctgca attgatttaa cagatgaatc aaaaggcatc tatggttttg
      721 catcacatat tggaacagag actggctact ggccatttat ttatcaaaat gggggagaag
      781 tatttgccga tgacaataca aaatcaggtt tgaatacccc agaagcacaa gaagctttag
      841 ctttttatcg agatattgca cttgttgatc atgcttcacc aacaattaca gaattacaag
      901 atcctgataa gttaagtcgt tttcaatcag gtagattagc aatgatgatt gaaggaaact
      961 ggtttgcacc gggatttttg aaaaatgact atctagttga aaatgttgct tctgccgtgt
     1021 tacctaaggg aaaagttaat gcaacaatta caaatggact tggttgggct gccagtgcga
     1081 gtacaaaata cccagaagaa gtgaaaaaac taatggcatt tttagctagt gaggacgcca
     1141 ataaaatcca agcggaaacg ggggcatcaa tcccagcact aaaaggcttt gaagacgagt
     1201 gggctgcttc ttcagataca attgatttga gtgcttttgg aaaaatgttg gagtatggag
     1261 tccctcgtcc atacaataaa gatggattac gagctgaaca agcagaagtt caaattatta
     1321 accgtattct gtctggtgaa gtaactgttg aagatggaac cgctcaaatt gaaaaagaag
     1381 tcaatgcgat attgagcgga aattaaatta gttaattata caatatacag gcgaaaggat
     1441 tctatttcgt ctgtatatta atggatgaat accaaaaagg gggatattat gaaaaatcga
     1501 atttttggaa aatatcgtaa agatcaagtg atatggggct atatttttat ttttccaacg
     1561 gcattaggat taattttatt aaatatatgg ccagcaattc aaacagcaat tttaagtttt
     1621 caaaaaacgg tagggttcgg tgatactgaa tgggtcggat taagaaatta ccaaaaaatg
     1681 tttgcagacg gtgaagtttt tcagtcttta ttaaacacat taatttatgc agtggtttcg
     1741 atacctagta ttgttgtatt atccttgttg gtggcagttt taatgaatag aaaagttaaa
     1801 ggattgtcga tttatcggac aatttatttt ctaccagtcg ttgctgcacc atccgcagtc
     1861 gcaatgattt ggcgctggat gttcaataat gattatggac tgattaataa catgttggct
     1921 aaaataggtc tcgatggacc agcatggtta acggatccat cgattgcgat ttattctatt
     1981 atcattgtag gagtttggag tgctattggt tataacatgg tgttattact agccggctta
     2041 taagaaattc ctaaagatta ttatgaagca gcggaaattg atggtgctag tccaatccag
     2101 cagtttttca acattacgtt accattagtt acaccaacat tattttttgt agttgtaaca
     2161 acagttatca atgctttcca agtatttgat gttattttta tgatgattac tcctaatagt
     2221 accgccatgt ttaaatcaca atcattagcc tatttattct ataaacattc atttatttta
     2281 aatgacaagg gatatggttc ggcaatcgtt atgttccttt tagtgattat attaattatt
     2341 actgcaattc aaatgaagtt gcagaaaaag tgggtaaact attaaggagg ctatcaaatt
     2401 atgaaaaata aatttttttc aaatgcgcca atccatatca tgcttatttt aggtggtatt
     2461 gcaatggcat taccttttat ttggatgatt ttaacttcag ggaaaacgtt ggttgaatct
     2521 acccaaattc cacctacaat tttccctgag aaatttcagc taagtaacta tcaacaagta
     2581 cttgatttat taccattcat ggctttttat atcaatacat ttttaatggt tgttggacga
     2641 gtgattggct cggttttatt ttcagcaatg gctgcatatg cttgtgcgcg tctaagtttt
     2701 ccagggaaaa atatcctttt cagcttagta cttattcaaa tgatgattcc cggttccatt
     2761 tttattactc cacagtattt attagcacaa aaattgggta ttttaaatac aattccagca
     2821 ttaatattac caggggtagt tagtgcgttt ggtacgtttt tattaagaca atttttctta
     2881 ggtttacctg atgaattgga ggaagcggcc tatttagaag gtgccaatat ttggacgatc
     2941 tttttcaaaa ttatgttgcc gcttgctaaa tcaggattgg ttgcacttgg aatattcaca
     3001 gcgttgtttg catttaaaga tttaatgtgg ccattgattg tcaatacttc aattgataag
     3061 atgcctttag ctccgggatt agcaaactta caaggacaat atgccacgaa cttcccacaa
     3121 ttaatggcag gttcgttatt agcaatttgg ccaatagttg tgatattcat cgttttccaa
     3181 agaaaattca ttgaaggaat tgctacaaca ggtagtaaat tataaaacta ttaattagtg
     3241 attggagagt aagtacatga caaaaattac atttattggt gcaggaagta ctatttttgc
     3301 aaaaaacgta ttaggcgatg cgatgttaac tccaagtcta caagatgcac atattgcatt
     3361 gtacgatatt gatgaaaaac gactaaaaga atcaaattta atgcttaaaa atattaataa
     3421 aaattcgaat gaaaatcgtg ctgatattgt ttcttattat gatcgcaaag aagcgttaag
     3481 agaggctgat tttgttatca atgctattca agtgggtggt tataaacctt caacagtgat
     3541 tgattttgat attcctaaaa aatatgggtt gcgacaaact attggtgaca ccatggggat
     3601 tggtgggatt tttagaaccc tacggacact accagtaatg ttagactttg ctaaagaaat
     3661 ggaagaagtt tgtccaaatg cttggttatt gaactataca aatccgatgg cgatgttaac
     3721 catgggtgtt ttaaaagcga cgagtattaa gactgttggt ttatgtcata gcgttcaagt
     3781 atgcgtacca gaattgtttg agcatttagg aatagccgat caatatgact taaatgactt
     3841 tcaatggaaa attgcaggca ttaatcatat ggcattcttg ttggaaatta caaaaaatgg
     3901 ggaagatttc tatccaacaa ttcgtcagtt agctgcagaa aaaccaaatc cacatccaga
     3961 ttctgtacgt tttgaattgt tgaaacgttt tggttactat attacagaat caagtgaaca
     4021 caatgcagaa taccatccat actttattaa atcaaaatac ccagaattga ttgacgaatt
     4081 agggattccg ttggatgaat acttacgtcg ttgtgaacaa caaattaatg attgggaaag
     4141 catgcgagag gacattgtaa ataatgggga gttaacacat acgagaagcc gtgaatatgc
     4201 ttcatatatc atggattcta ttacgacagg aactccgtca gtgattgcag gaaatgtctt
     4261 aaataaaggc ttaatcacaa acttaccgac agatgtttgt gtggaagtac cttgtttagt
     4321 cgataaaaat ggcgtgcaac caacctatgt cggtgaattg ccaacacaat tagctgcttt
     4381 aaatcggacg aatatcaatg ttcaagaact gacagtagaa gccgctttaa cactagataa
     4441 atcaaaaatt tatcaagcag cgtatttaga tccacatcta tcatctgaat tgtctttaac
     4501 agatatcaaa aatttagtgg atgatttaat cgaagcacat ggggactatt tacctgaata
     4561 taattaaaaa gaagctgaaa gagtaaggaa ttttcaattc cttactcttt tcttagttaa
     4621 atttagtaaa aatattgaca aaacacttag taaacagtta cattagtgtt aacaaaaaga
     4681 aaacgcattc aaattgtata aggaggagca taaaatgaag tggtctaaaa aaattatttt
     4741 agcttgtata gcactaggag caacgatctc tttgactgct tgtggaaaaa atcaggaaga
     4801 tgcaagcggt aaggtagaaa tagagttttt taatcaaaaa aaagaaatga ctcaaacgat
     4861 tcaggagatt gctaaagatt ttgaagcaaa aaaccctgat attcatgtaa aagttgtgga
     4921 tgtaccgaat gctggtgaag taattaaaac acgtatgctt gcaggcgatg taccagatgt
     4981 cattaatctt tatcctcaaa gtatagaatt gaaagaatgg gcgaaagctg gttatttaga
     5041 ggacttaacc gaagaaccgt atttagagaa tatcaaaaat ggatatgcac aacgttttgc
     5101 tattgaggat cgtgtatata gcataccatt aaccgctaat gtctatggtt tttactataa
     5161 caaaactgct tttgaggaaa tggggattca agcaccagaa acttgggcag aatttgaaaa
     5221 aatagtagct gagattaaag atcaaaacaa agtaccgttt gcgattgcag gagcagaagg
     5281 ctggacttta aatggttatc accaactagc tatagcgact gctgctggtg gtgaagagga
     5341 agcgaacaat gtttggcgtt tctcagaagt taatggaatt aatgctgaaa gcaaagaaat
     5401 gcaaaaagat tttaatcgat tagatttgtt aagagaacca aaagctttgc aaaacaactg
     5461 gcaaggtgcc ggttataacg atacagtagt tacttttaca aaaggtgatg cgttgattat
     5521 gccgaatggt tcatgggcaa tgccgatgat tcattcacaa aatccagatt ttgaagtagg
     5581 aacttttcct tttccagcag atgaagcagg tcaaagtcta acgattggcg caggagacct
     5641 agccttatca gtttctgcaa cttctcaaca taaagaagaa gccaaacgtt ttgtagaata
     5701 tatgacaaca cctgaagcga tgcagaaata ttatgatgta gatggtgcac cgtgtgctgt
     5761 tgagggcgtt atcgagaata ctcaggattc accattaagt ggtttaactg aacttgcttt
     5821 tactgatcgt catttagttt ggttagccaa agattggaat agtgaaaatg atttttatac
     5881 cttaacaaca aattatttac acaatggcaa tcaaaaagcc atgattaacg cattaaatgc
     5941 tttctttaat ccaatgaaag ctgacggaag gtaggaaaat aaaatgaaaa taaaagaaat
     6001 tttaaatcgt tactgggcaa tcatttttgt agcaattcca attattttgc agatgatttt
     6061 tttctacttt ccaatggttc aaggtgcttt ttacagttta actaattgga ctggtctgac
     6121 ttataatttt gattttgtgg gtttgaacaa ttataagatt cttttttctg atccgaagtt
     6181 tatcaaatcg attggcttca ctttaattgt gacgctttgc atgattgctg gacaaattgg
     6241 ttttggtatt ttgattgcgc gggcgttgaa cgcaaatatt aaagggaaaa cattctttag
     6301 agcatggttc ttttttccag cggtcttatc gggcgtggtt gtttctttag tatttaaaca
     6361 aatttttaac tatggtttac caaaaattgg tagtatgtta ggcattgaat ttttacaaac
     6421 cagtctttta ggaacaacat ggggagcgat ttttgcagta atttttgttt tactttggca
     6481 aggaattgcc atgccagtca tcattttctt agcgggtttt caaagtattc cacaagaaat
     6541 tattgaagct gctagaatgg atggggcgaa tgataagcaa attttttgga atattgaaat
     6601 cacctactta ttaccaagta tttcaatggt atttattatg gcattaaaag gtggcttaac
     6661 agcatttgat caaatttttg ctttaactgg tggtggacca agcgatgcca caacatcttt
     6721 gggg
//
LOCUS       DACDSY010000143         6577 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDSY010000143 DACDSY010000000
VERSION     DACDSY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.145"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            168..482
                     /locus_tag="IWK44_002441"
     CDS             168..482
                     /locus_tag="IWK44_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9550912.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            503..880
                     /locus_tag="IWK44_002442"
     CDS             503..880
                     /locus_tag="IWK44_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9550913.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            890..1663
                     /locus_tag="IWK44_002443"
     CDS             890..1663
                     /locus_tag="IWK44_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550914.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            1685..3088
                     /locus_tag="IWK44_002444"
     CDS             1685..3088
                     /locus_tag="IWK44_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9550915.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3270..4454
                     /locus_tag="IWK44_002445"
     CDS             3270..4454
                     /locus_tag="IWK44_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9550916.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4451..4741
                     /locus_tag="IWK44_002446"
     CDS             4451..4741
                     /locus_tag="IWK44_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550917.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            4738..4959
                     /locus_tag="IWK44_002447"
     CDS             4738..4959
                     /locus_tag="IWK44_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550918.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5001..5780
                     /locus_tag="IWK44_002448"
     CDS             5001..5780
                     /locus_tag="IWK44_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP9550919.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            5847..6341
                     /locus_tag="IWK44_002449"
     CDS             5847..6341
                     /locus_tag="IWK44_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9550920.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6397..>6577
                     /locus_tag="IWK44_002450"
     CDS             6397..>6577
                     /locus_tag="IWK44_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550921.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKE"
BASE COUNT         2259 a         1074 c         1307 g         1937 t
ORIGIN      
        1 aggaatcgga cgctcagtac ggagcgtcta tttttttacc caattttgaa aggaagtgaa
       61 cttatgaaaa taaaaaatca agaatcaaaa ggtcgctctc ccccctttaa aaccatcaca
      121 caaataagat tcattcaaac ataaaaacag aaaggatagg taaaaatatg gaacttaaat
      181 atgtcattcc caatatggaa aaaacatttg gacaattaga atatgctggt gaagggaaca
      241 ttgaacaacg tcgaataaat ggcagaccaa ccattctctc tcgtagttac aatttatact
      301 caacgattca acgagcagat gatattgttg tcattcttcc ctccgaagct ggcgagaaac
      361 attttgaatc cgacgaaaaa gtcaaactca tcaacccaca tattaccgca gaaggttata
      421 aaattggcaa tcgaggattt accaattaca ttatgcacgc agacgatatg gtgaaagcat
      481 aagaaaggaa aggtgaaaaa atatgagatt agcagaaggt attgtgattg aaaaagacca
      541 gacatttggc acattgaaat tttccgcctt gcgtcgtgag gttcgtatcc agaatgaaga
      601 tggtacggtt tccgaagaaa ttaaagaacg tacctatgac ttaaaatcaa aaggacaagg
      661 acgcatgata caagtcagta tccccgcttc tgttcctttg aaagaatttg actacaacgc
      721 agaagtggaa ttaatcaatc ctgtggcaga taccgtagcg acggctactt ttcgaggggc
      781 agacgttgat tggtacgtga aagcagacga tcttgtatta aaaggacaga caaaacctca
      841 tcagaaatat aaaaaagaag cgtccgaaag tgaaaaatag gtgttgctta tgacaaacac
      901 accattgatt ttgaaagaaa ttccgaagga tgaagccatt tcttttattc ggcaatacca
      961 ttattcaaag atactcccta gattgtgtaa atactttcta gggatttttt cagaagaaaa
     1021 attattaggc gttgtggaat taggctgggg tacgcaaccc ctacaaacca tacgcaaact
     1081 ctttcccgac agttcccttc aaaccacaga ttatttagaa attggaaaga tgtgttttct
     1141 tccagaaatg aatcaaacca attattttgg cagtcaagct ctttcagctc taattaagtg
     1201 gttgaaagaa catacggact gtcatttttt atatacactg gcagatggaa ttgagggaaa
     1261 atgtggctat gtctatcaag cgtccaactt cttttactgc gggtatttca agacaagtgt
     1321 ctatcgtgat aagcagtcat gggaaaagat acaccctcga agtgctcgcc ttttattaga
     1381 ggaaaatgct cgctttgaac aggtagagaa aaagcattgg ctttcccagg cattttgcga
     1441 atacaaaggg attgagaaaa tcaatggacg catgtttcgc tatctctatc cgttgacaaa
     1501 agaagcgaaa aaactgttag gacacactct ctatcgacgt cattattatc cgaaagaaaa
     1561 aaatttacgc tttgaaaagc gaatcgctta tcagaaatat gaagccattt ctcaaccaac
     1621 ttttgataaa caggctcgta tctacaatac acaattattt tgataggtgg aaaggaggat
     1681 ttttatgcca ttgcttcatg acagaggaga gcgaatcaaa gccaacgata agcacttggt
     1741 ctatcatttt gtactaattt cattgctggt agtttttcta gtcgtcctac tcccttttca
     1801 ttggagagcg ttacaatcgg tgaactggca agcagttcag ttagatacat ttttctatca
     1861 acttcatacg ccatactttt ggagcagtat tctcactgct ctttttgtat gcggacttat
     1921 cagctggctt tattatcact atcgtataga ttctataaaa cagttagagc accgtcaaaa
     1981 gttagctcgt atgattttag aaaatcattg gtatgaagcc gaagaaactc aatcagaggg
     2041 attctttaaa gatttaccca gcaagaaaac caaacaacga atccgccatt ttcctaaaat
     2101 atattatcga atgaaagatg gactaatcca tattcatgta gaaatcacct tagggaaata
     2161 tcaagatcag cttttgcacc tagaaaacaa actagaaaca ggattgtatt gtgaattagt
     2221 ggaaaaaacg ttgaaagatt cttatgtaga gtatgtccta ctttatgata ctattggcaa
     2281 tcgaattggc attgaagacg tggtagctca agatggacgt gtcaaattga tggaatcggt
     2341 ctattgggag ttcgattctc tccctcacat gttaattgct gggggcactg gtagtggaaa
     2401 atcctatttt attctcacat tgattgaatc tctcctgcat acaaatgcaa agctttatat
     2461 tcttgaccct aaaaatgctg atttggcaga cttaggggcg gtcatgaata atgtctatta
     2521 ccgtaaagaa gatatgttgg catgtatcaa tcaattttac gaagatatga ttgatcgcag
     2581 tgaaaccatg aaacaacacc ctaactataa aacaggagaa aattatgctt atcttggttt
     2641 gtctgccaat tttcttattt ttgatgaata tgtggcaatg atggatatgc taggtcgtga
     2701 aagtaccacc gttattcata aactcaaaca aattgtaatg ttaggacgac aagcaggctt
     2761 ctttttaatt cttgcctgtc aacgtccaga cgcaaaatat tttagtgatg ggattcggga
     2821 caactttaac ttccgtgtgg ctttgggtcg tatgtctgaa atgggctttg gtatgatgtt
     2881 cggaagcgat acacaaaaac aatttttctt aaaacccatc aagggaagag gttacgtgga
     2941 tacaggaaaa agtgtgatta gtgagtttta tacacccctt gtaccaaaaa ggtacgattt
     3001 cttaggagaa ataggaaaag tcattcaaaa aaagcaatct gagcctgtac cgcacgaagt
     3061 gaaaggtaca ggttcagact tgctttgatt ggtgtggcgt tagccacgcc aatcctttag
     3121 ccccctctat ctaacagggg ggcacaaata caactgaaaa caggcaattc tcatcaacca
     3181 atgttgatgg tacaaaggat tgcggaaaat atgaaaggtg taaactcaag ggtcatagtt
     3241 tacatctttt ttagattgga ggtttgcgaa tggaaaacga aaaaatttgg attaaggaat
     3301 tgaaagaaaa gcgactggct tatggcgttt ctcaaaataa actggcggtt gcttctcata
     3361 ttaccagacc ttacctatcg gatattgaaa caggcaaggc agtccctact caacaggtga
     3421 aagaagattt actgaacgct ttggaacgct tcaatcccga taacccattg gagatgttat
     3481 tcgactatgt acggattcga ttccctacca atgatgtggc tcaaattatc ggtgaggttt
     3541 tacgtttgaa tatggattat atgctacacg aagactttgg ctattattct tatcccgaac
     3601 actatcgttt cggtgatatt gttgtcctag tctctcatga tgtaagtaaa ggtgttttat
     3661 tagaactgaa aggaaaaggc tgtcggcaat ttgaaaactt tctattggct caacatcgga
     3721 gctggtatga tttctttcaa gattgcatgg aacataaagg tattttcaaa cgcttggatt
     3781 tagctatcaa cgataaaaca gggattttga atattccaga tttggcaaag aaatgtaaac
     3841 aggaagaatg catttccgtc tttcgaagct ttaaaaatta tcgcagtggt gaattggttc
     3901 accgtgatga aaaaccatac atgggaaaca cactttatat tggctcatta aaaagtgaag
     3961 tctatttctg tatatatgag aaagattatg agcaatacgt gaaaaatgat gttcccttag
     4021 aagacgcaga agtaaagaat cggtttgaaa ttcggttaaa aaatgaccga gccacccaag
     4081 ccatgaaaga tttattggca catcaacagg cggaaaaaac agcctttgaa attatcaatc
     4141 gctatatccg ctttgcagat aaagacgaca ctaaacgtcg gagtgattgg aaaactaatg
     4201 aacgttggga atggtttatc ggtaaaaatc gtggagcatt acgcctaacc acacagccag
     4261 agccttattc ttttgaacga acattgaact ggttacatca tcaagtcgcc cctacattaa
     4321 aaatcgcaag cattttagat gttctcaacg gtacaaccat tatctccacc atgattcagg
     4381 aagccaaact gacagaaaaa catgagaaat taattgaaca acaacatcta gccatggagg
     4441 atttgattac atgagccttt taacaaaaga attaaagaag ttagggtttc agtgtggcat
     4501 tgagtttcaa gcgtatatac aaaatacagg gaaatatacg tctctcatta ttgagggaaa
     4561 acggcaagct ggtgatacca tctataccta tgatttttac aaggtgagat tttataataa
     4621 ttataccaat cgagtaaccg tctatggaga gcatttaacc ccttttcaat tattaaggcg
     4681 tgtaaaaagc tatatctatt atcgagaaaa atatcttaag gaaaggagga caatcacatg
     4741 aattttgggc agaatttgta caactggttc ttggataatg cacagtcatt ggtattgtta
     4801 gcgattgttg tcattggact gtttttaggt tttaaacgag aattttcaaa actcattggc
     4861 ttcttgatta ttgctctaat cgccgtcgga cttgtgttca atgcagctgg cgtgaaagat
     4921 gtgttattaa acttatttaa ccgaatcatt ggtgcttaac agatattttt catgataaaa
     4981 taggaagtat atattttgtt atgaaaggat ttatgaatat gtttacaaat gatcaattta
     5041 aatctctttt aattaaaaca ttagaaagaa agagctcttc aaattattat gaaggaggta
     5101 acgagattgt ttctaaaatg aaaatatcag aggtaacttt agatgagact gacgatttta
     5161 cttattataa aggatataaa aagggctgga atacgctttg cacatatctt aaaataagag
     5221 ttccttttga tgatttagac ttttttgaaa gtcataaaga tttgattacc cagacagcaa
     5281 gctccatata tgataaacaa ggtgataatg tattagtgga tactatttta gtaccacttc
     5341 cagagaatta cgaagtaatt aactttagtc agttgaaaat tagtgatgtg gtttcacaag
     5401 ctattgaaga cgctgagtct tttatgagta atggtgaata tcaacgtgcc tttgacagag
     5461 ttcacactgc ttttcatgga tatttaattg aaatactaaa aaaatatgaa attacagttc
     5521 ctagagacga aaatctatct aaattatata gtaggataca acaattaatt gaaaaagaaa
     5581 ttcaacctac tgaacttgct gatatagtga agacaacgat aaggtcatca aatggaatga
     5641 ttagctctct taatgaagca agaaataggc attcacttgc acaccctaat acaaatatta
     5701 ttggaaagag agaagcaaaa ctaataatag gtatttcaag tacagttaca gattatatta
     5761 gcggatacct tgataagtag cgtcattaac tgattgctac tttttttatg actattttta
     5821 taaaaaggat gtgataagaa ttggaaatga aagtctatgt ggcaaattta ggacggtata
     5881 atgaaggtga attagtcgga gcatggttta ctccaccaat tgatgaggaa gaaatggcag
     5941 aaagaatcgg tttaaatgag gactacgaag aatatgcaat acatgatttt gagcttcctt
     6001 ttgatgtgga cgaatacacg ccgattagtg aaatcaaccg tctatgtgaa gccattcaag
     6061 aaatcgaagg tacacctatt tataatgaac tcaaagaaat tcaaggcatg tggtttagta
     6121 gcttggaaga attactggaa aacaaagagg atattcattg ttatagtgat tgtgattcga
     6181 tggaagatgt cgcccgttat tacgtggaag aaacaggaca gcttggtgaa gtcccttcca
     6241 atttacaaaa ttatattgac taccaatctt tgggacgtga tatggaaata gagggtaatt
     6301 acctagttac ctctcatggg gtatttgaat attgtcagta agtttaacaa tccgtgttat
     6361 aattaaatta ataaatagca cggagaggta taaattatgg taaatgtatt tgaaaatctt
     6421 aaactattaa aactagacat gaaagataaa ggctgggtga tagattcctt ttactttcgt
     6481 tacaaacagc aaaattatat tgtacttgtt aagctatttg aaaaagagga aaaagttcca
     6541 gaatatgctc tgttaaaact agaattttta aaggaaa
//
LOCUS       DACDSY010000144         6419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDSY010000144 DACDSY010000000
VERSION     DACDSY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.146"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1431
                     /locus_tag="IWK44_002451"
     CDS             <1..1431
                     /locus_tag="IWK44_002451"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9550922.1"
                     /translation="EYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFN
                     VLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEV
                     IKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQK
                     ERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAE
                     EMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSK
                     ADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLF
                     FGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVE
                     KYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLE
                     AISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1421..2638
                     /locus_tag="IWK44_002452"
     CDS             1421..2638
                     /locus_tag="IWK44_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9550923.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2651..5800
                     /locus_tag="IWK44_002453"
     CDS             2651..5800
                     /locus_tag="IWK44_002453"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAP9550924.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         2241 a         1066 c         1318 g         1794 t
ORIGIN      
        1 gaatatgaca cagtaagcaa acaaacaatg ttgtatcgtg aattactttc agatgaagaa
       61 tcaaaagaag atctgattgc cacaatcgtt gatattttgg gatatgcaat tgcaccagag
      121 tatcttttca acgtcttagc ggatcaagca aaacaagcaa cgttccaatt gaacgatcta
      181 aacaaagcct ttgtgcaatt ggcatctacc tacaatcaat ttaatgggtt atttgatgat
      241 gtggatctcc aatcaaaaaa actaggaaca gatgagcaac aacgaaatgt gacgattacc
      301 gaagtcatca agaaattgaa tgatgtagat gttttagaac acgatgggga cgtgatcggg
      361 gatgcgtatg agtttctgat cagtcaattt gcttcagaag cagggaagaa agccggagaa
      421 ttttacacac ctcatatggt ttctgatatg atggcgcaaa tcgtaacgct cgatcaaaag
      481 gaacgacgat tctttagtgt ttttgatcca acgatgggtt caggttcatt gatgttaaac
      541 gtacgtaact acttaacaca cccagataac gttaaatatc atgggcaaga actaaataca
      601 acgacataca atctagcaaa aatgaacttg attttacatg gtgtcgacgc cgaagaaatg
      661 aatctccgca atggggatac gttgaataag gactggccaa cagatgaacc ttacacattt
      721 gatgcagtag taatgaaccc accttattcg gctaattggt cagcagatac gactttctta
      781 gatgattctc ggtttaatcg ttatgggaaa ttagcgccaa aatccaaagc agattttgcg
      841 tttcttcttc atgggttcta tcatttgaag gaaacaggta ccatggccat cgtcttacca
      901 cacggagttt tattccgtgg cgctgcagaa ggggtcatcc gtcaaaaatt actagaagat
      961 ggcagtattt atgctgtgat cggtatgcct gcgaacttgt tctttgggac atcgattcca
     1021 acgacggtga ttgtgttgaa gaagaatcgt caaactcgag atgtattatt cattgatgct
     1081 agtcgtgaat ttgttaaagg gaaaaatcaa aataaactat cggaagaaaa cattcaaaaa
     1141 atcctcgaaa cctacgctga aagaaaagat gtcgagaaat acgctcattt ggcaaccttt
     1201 gatgaaatca aagaaaatga ctataactta aatatcccac gctatgtaga tactttcgaa
     1261 gaagaagaac caattgatat ggttcatgtc ggaaatgaca tcaagaagat acgacaagaa
     1321 cagcaagtgc tagaaaaaaa acttttagaa gctatctctt ccctgcaaac aacacctgaa
     1381 aatgaagcgt ggttacaagg tgcgctggag gtgttcaaac atgagcaatg atacacaacc
     1441 agaaatccgt ttcccaggat ttacagatga ttgggaacag aggaagttgg gggacttcgt
     1501 tgtagattat gttgaaaaga caagtgtaca aaatcaattt cctatgctaa cttcatctca
     1561 acaaaaaggg atagttttgc aagaagacta ttttgcaaat cggcaagtaa ctacagaaaa
     1621 taatattggt tactttgtac tacctagagg atatttcacg ttccgtagtc gaagtgataa
     1681 tgatgtgttt gtgtttaacc gtaatgacat tattgataga gggattataa gctattttta
     1741 tcctgtattt actttaaaat ctgctgattc cgacttcttc ttaagacgta ttaacaatgg
     1801 aatccaacga caattatcta ttcaagcaga gggaacaggt caacatgttc tgtctctaaa
     1861 aaagtttaaa aatatagtag caatgtttcc tagtgaggaa gaacaacaaa aaatcggcac
     1921 cttcttcaaa caacttgacg acacgatcgc tcttcatcag cgtaagttag atttgctgaa
     1981 ggaaacgaaa aaaggattct tacaaaaaat gttccctgaa aacggagcaa aagtgccaga
     2041 aatccggttt cctggtttta cggaagattg ggaagagcgt aagttgggag aattaggtaa
     2101 aactcaatcg ggaattggat ttcctgatgc cgagcaaggt ggttctgaag gtactccatt
     2161 ttttaaagtt tccgacatga ataacattgg taatgagtat gaaatgagaa atgcaaatca
     2221 ttatgtatct aatgagcaaa ttgagcgtaa aaaatggaag ccaataaaag atgtgccagc
     2281 gattatattt gctaaagttg gcgcagcaat aatgcttaat agaaaacgtt tggtcacaag
     2341 ccctttctta atagataaca ataccatggc ttatttattt aataatactt gggatatcta
     2401 ttttggtaaa atattgtttg aaactataaa tttgccaaga tactcccaag taggggcatt
     2461 gccttcttat aacagttctg atattgagaa tatcagtgtt aaggttcctg taaaggatga
     2521 acaacaaaaa atcggtactt tcttcaaaca gctagatgat acgatcgctc ttcatcaacg
     2581 taagttagat ttactaaaag aaacgaaaaa aagcttctta caaaagatgt ttgtctagga
     2641 gggaagaaga atgaaaatta gtcaccgtga ggaagcagaa gtcgaagaac agttgattcg
     2701 tgtacttgga gaagggcata atcagtggac gtatcgtccg gatctaaaat cagaagaaga
     2761 cctttgggtc aatttacgcc aaaaaattat ctcaaataat caagcagaac tgaatgattt
     2821 tcctttgacc gataaagaat ttgaaacgat caaaacggaa ttactgctgc gaacgaaaac
     2881 cccatttgat gctgcgaaat ggttaaaagg tgaaaatggg atggctcgga tcacgattga
     2941 acgggaagat tcgcaattgg gttctgtctc acttatcctt tattccaatc aagacattgg
     3001 tggaggaatc tctacttatg aagtggtcca tcaaattgca aaacgtggaa gtaacataga
     3061 agcacgggat cgacgttttg acgtcacctt attaatcaat ggattaccga ttgtccaaat
     3121 cgagctgaaa caagtaactg caaaagatgg tgtttatcag gcatttaacc aaatcaaaaa
     3181 atatgcagaa gaaggtatgt ttcggaacaa tatcttttcg accttacagt tgtttgtggt
     3241 ttccaatgaa caaaccactc ggtattttgc aaatgcctta ccgaaagact tgcaccctaa
     3301 gtttttattt agttggcgaa cgaaagacaa tgaaaaagta gaaaacttat atgagttttg
     3361 taaacaagtg ttaaatatcc cggatgctca tcgtttgatt gcggattata caatcgtcag
     3421 tgaagatcaa gacaataaaa ccctcatggt gttacatcct tatcaagtac atgcgattca
     3481 agccttgttt attgccgcaa ataaacatca atcagggtat gtgtggcatg cgactggttc
     3541 aggaaagacg ttgactagtt ttgtatctac taagctttta gcacgaaaat cggggatcga
     3601 tcgaacgatc atgttagtgg accgcaaaga tttagataat caaaccacca cagagtttac
     3661 aaaatttgct tcggaattta atactgggat ttcttccggg aacgccaaag cgaatagctt
     3721 gatcgttgga acgggaagtg ccaaagaatt aagtgaaacc ctgttggcag atgccaatgc
     3781 aaatgttgta atcattacaa cccgtcaaaa actagatgcg gcgttgaaat atgcaaagaa
     3841 acaagaagag aaaaaaggaa ccaatcgctt ccaaaaatta atgggccaac atatcgtgtt
     3901 tgtagtagat gagtgtcatc gagcgttaag tgctgaaaat atggaagaaa tcaaaaaaat
     3961 gtttccgaaa tccacttggt ttggctttac aggtacaccg atttttcctg agaatcggaa
     4021 acaagccaaa gggcaattgg cacgtacaac ccatgatcag tatggagagg tcttgcatac
     4081 ctataccatt aagaatgcgc tagaagatgg ctctgtctta ggatttcaag tagagcatga
     4141 aaacaccgtg gagccgactt cactagagaa taaaatctat cggaaattaa aagaagtaga
     4201 aacatatgca gagtattcat cagaacagat caatcgtatg attgatcaaa tggaacctgt
     4261 caaaaaagaa agttatttgg acccagctgt ttttgaagca gatgaacata tccaaaaagt
     4321 cattcataaa atgttccgtc cagataatgc ctatacaaaa tttgattttc gaaatggcag
     4381 gccaacgaaa tctgcaatct taaccacgag ttccattgac atggccaaaa agtattatcg
     4441 ggccatcaaa gagatgacga aagagcctga ttggttaaca aaagagtttc ccaatcagct
     4501 cattcgtgaa ggacgaacga tggaggatcc tgattttcca cgaatcgcta ttacctattc
     4561 attagaagaa aatagccgag attcaagtgc ccaacaagaa gaaatgcaaa aaattattga
     4621 ggaatataat cagtattatg atacttcctg gtcattagca gacattgagc gttataacgg
     4681 agacatcaat aatcgtttgg cacgaaaaag agcggaattc aaacaatttg ggaaacaaat
     4741 cgatttagcg attgtagtgg atcgtctatt aacaggtttt gacgccccta cgattcaaac
     4801 cctatttgtg gatcgtaatc tagagtatgc aggattgatc caagcttttt cacgtacgaa
     4861 tcgtacgtat cctgataaga caaaaggatt gattgtgacg tttcgtaagc cagcgacaat
     4921 ggagaagaat gtcgaggatg cgacaaaact gtattcaaaa gcaaaagaag aatcaggttt
     4981 gatttatcca agttatcagg aatccaaaaa aagattcaaa caagcatatg gtaagttaaa
     5041 agaggtttca tccatggaat ccattgatga acatacgcca cttgaaatcc gtatcgagta
     5101 cgtcaaagca tttcaagaat tgaatcgtgc ttatgaatcg ttagtgactt atgacgatta
     5161 caatgatgac atggtgaaat ctccaacttt gaacaatcaa attcaacttt tggaagaaca
     5221 aattggcgtg tacgagaccg tgaagggttc attaatagag gaagaaccag aaaaagaagg
     5281 agaagatttt tcaacgatcg aattttatag tgaaaatacc actaagttgt atgatattga
     5341 ttcaacttat attgatcaat tactaggaac ttatgctgcg aatagttcag atgcacgaga
     5401 agaaattgaa aaagctttgg ctaaattgaa taaaacagaa ggagtcaaag aagtttaccg
     5461 acaaatttta aatgcatttg atgtggggac tttagatagt aatgaagata tctttgtcat
     5521 taaacgtcgc tattttacac aagcgagtga tgatttgatt catgtctttt ccaatgagtg
     5581 gtttgtttct gagaaagaat tacatgcttc taatctgcaa tatctaccag gtgaagatcc
     5641 aattcctaat atgaaagcaa ttatcaacag taaggattac gaaggatata aagccaaaca
     5701 tccagaagcg aagccattta aatacccaca agaaatgaag cgagcatggc gtaaaatgtt
     5761 agatgatgag ttgattccac ttgagaatga attgaggtaa acgagaagag ttaagaaaat
     5821 tgtacgattc aaagatggta gtcaagtgga agtgtagaaa atagggatag atgtctttta
     5881 tttgttcaca ttttataacg aagtatttaa gacagctatt gaataatgtt tcctccacct
     5941 ttttcattca tttaccccac cccgttgtta tatgaccgtg ttttgagagg aaccaataaa
     6001 aagcataggc taactagggg ttcctttttc ttttgttttt ttggaatccg cttaagtgaa
     6061 atgtatctga aatgaaacgg agataaagtg attttttgct ataatgacaa ggttttagct
     6121 gtttcagata ccatgtaacc caaaatagtt tattgagaaa ataggagagt atttgatact
     6181 aactagtagg aaaaaatgag ataaaaatta aatggaatca tttcaggtaa tctcaaataa
     6241 aaaaatatac agtcctgtgg cactttacct atgggactgt atatttttta ctctcagcgg
     6301 gcttgttttg actccgtgtc tgactccgtc gtattgattt taaatgatat tcgatgacca
     6361 tttaacaggt catttttgaa atttttgtct ctatatgact cagtttgata attattgat
//
LOCUS       DACDSY010000145         6367 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDSY010000145 DACDSY010000000
VERSION     DACDSY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.147"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..317)
                     /locus_tag="IWK44_002454"
     CDS             complement(<1..317)
                     /locus_tag="IWK44_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9550925.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLT"
     gene            complement(314..835)
                     /locus_tag="IWK44_002455"
     CDS             complement(314..835)
                     /locus_tag="IWK44_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9550926.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(837..1871)
                     /gene="sppA"
                     /locus_tag="IWK44_002456"
     CDS             complement(837..1871)
                     /gene="sppA"
                     /locus_tag="IWK44_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9550927.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(1904..3319)
                     /locus_tag="IWK44_002457"
     CDS             complement(1904..3319)
                     /locus_tag="IWK44_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP9550928.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(3288..5441)
                     /locus_tag="IWK44_002458"
     CDS             complement(3288..5441)
                     /locus_tag="IWK44_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAP9550929.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5699..5935
                     /locus_tag="IWK44_002459"
     CDS             5699..5935
                     /locus_tag="IWK44_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP9550930.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            5954..6169
                     /locus_tag="IWK44_002460"
     CDS             5954..6169
                     /locus_tag="IWK44_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP9550931.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
BASE COUNT         2021 a         1150 c          947 g         2249 t
ORIGIN      
        1 gttaaaagaa aaaatggaat aatagataaa atgagtaaac taataccata gaccactgtt
       61 ttttgatttt ttcttactaa aaaacctatt atgaaaatca ttatcgaaaa aactagggta
      121 attcctccaa ataaaacaac attcccgctt agattgacta acatatctat atccttactg
      181 gttagttctg tagtatttaa agtagttgaa tcaatagaag atagaaacat accacccatc
      241 aatgctaaaa gactacataa aatccctgtt attccactaa ttaaaatcaa aataaactct
      301 gtttttctct tattcattta attctctcca gattaagtgt tttagaatta gaaatttttt
      361 gaccatagtt aatagcttct aatgaacgaa taacaaccac tgacgtgtta gaaaaataat
      421 ccccaatatg ttgtttcttg ggcgtgaaag cagccaccaa atacccaatt tgaaatacaa
      481 aattttttaa tataaaccta catatacact cacgaataaa gatagtttgc caacttagct
      541 ctttttcctc aaagctcact actcttatcc caaaaatcat tttcccaatt gtttgtcctg
      601 aattcagctt agtcaaaagt acaaaatagg ttaaataaat agctaatgac gctataccat
      661 aaaaagtaat aatggatttg tcagattcca gtcccatgat tttgtaaaag agacctatag
      721 tagcatgggt gatgatgcta atacaaatta aatcaataag aaaagcaaaa aatcgtaacc
      781 aaaatcccgc aaatacataa ttaggcaaag tttcaatgcc actcttctta ttcattttat
      841 agccctccat agtaatacat tggttttgga gtatcagaaa atccaagttt ttcaaaaact
      901 gaaattacac gatctgatgt agttgccttt agcccttgcc attcagcaat cttattacct
      961 aaccaagaag aagcaaagcc agttgtgtca tttttatatt ctataaccgt cgcatcagtt
     1021 aatttcttct ctttttttag ggaatctaat gcatcttcag agtaaccaat ttgatccacc
     1081 aacccatttt ctactgcttg gttaccatca taaattcttc catccgcaag ttctttcact
     1141 gcattagtat ccatatttct tccttttgct acaacattga caaaacgatc ataagcactc
     1201 atcacaaatt cctgtataac tttattctct tcctctgaag gtttgtgttg aggtgacatc
     1261 atatctttta atgcaccact tttatacgtc gcatcagtta cgccaagttt ttctaataag
     1321 ccagaataat ttaaaccaga aataatgaca ccaattgatc cagttgttgt ttcttccgta
     1381 gcaaatattt tatctgaacc agcagaaatg tagtaccctc cacttgccgc tgtattttta
     1441 aatgcagtat agataggaat atctagcttt ttaatttttg ccatttcttt agcaatctca
     1501 gcactttcat atattcctcc cccaggtgaa tttacttcta ataacactcc cttaaccgct
     1561 ttgtcttctt gaattttctt tagctgtgtt aaaaaattct gatggttata ttgttcccta
     1621 ctaaaaagat tgctttcacc tgtatctgca ataactccat tgacggatag tttgacaatc
     1681 tttttatttg atgcgccttc ttctaacgtt tcttctgtga tttcgttaga tccgtaaaaa
     1741 atcttattta ttcctgttag ggttgtattt gatgcttcct ctttatcttt tcctggaatt
     1801 agcgcgacga taatggatag taacaataat ccgcacgcta ctcccacagc aatccatctt
     1861 cttttattca tataaactcc tcctatattc ttagtaaaaa taattatttt ttatttagaa
     1921 acacatcgac aatttgttgc caatatgttt tttctcctac aattaaagac aactctccag
     1981 ttaatccata acgaaacgga aacgattgag ttgtttttaa cgttcctttc actgcataaa
     2041 aaacaccttt ctcagtggca gtagaatttt ctgaaatttc atctaaagtt ccgtcgatcg
     2101 taatcggagc cccacctttt tgatccaatt taaaatgaac cttcatccct tctttaatat
     2161 aggtactttc attcgcaggt aaaagtgctg taaatttcat ttttgatttt tcgtggatcg
     2221 gataaatttc tgcaagcact gttcctttgg ctagttctgc ttgtcccttt gtttcttcat
     2281 ttaaatgaac cgttccagat acaggagctt ttagtattcc ttttgaaagt tgatcgtcca
     2341 aacttttgat aactgactgg ttcttctctt gtttttcatt tagctctgta actgtttgtt
     2401 ttacagttgt tattgcttgt tctagctctt gctttccttt ttccgtttga ctaattctgt
     2461 cattctcaga agtagttggt ttaactaatt tagctttttc tgactgatac tgttctactt
     2521 ctttttttag ttgctcaatt tgttcattta tgcttgctga aattgtcagt ttcacttgat
     2581 ttttttgatc atcagaaaca ttatttagtt gttcttgcca attctcatag tttgccatca
     2641 tttcttttga aaaatctttt aatgattgat tattactcca agctacctgt acttgttgcc
     2701 attcttgtaa tttactatct gaactctcaa tttgtttttc tagttgtctt tttgtctttt
     2761 cgtatacttc taattgcttt tgatcattta attcactttg cttcaaagcg tagcggagtg
     2821 attctttttc tgacaacatg ctctttaatt gattactgta gccaaaactg tcatccgttg
     2881 aaaacaaatt ttttccttta cttatacttt caataaatag ctgagccatc tttatttgtt
     2941 gagtaatacg ttggttttct tgttctatct gagccttttc attctctatt aaagaacaat
     3001 ccaatctaac caacacatcg tccttatgaa caaattgatt ttcttttagg aagttttcca
     3061 ttattttagc ttctaatggc acttgtaact tctctatttt ttcggggttc aattgagccg
     3121 ttgtccgaat aacaacctct tttttagcaa aggttaaaaa caatccaata atacataaaa
     3181 aaagaattac agggtataac acccaacggt agaagctatg atgttgttta gtatagatac
     3241 tagaatgttc gaaccaatta ttattctgca taaattgttt ccctctccta gatttcccat
     3301 aatttttgat agataccatc ggcaaaacga agttcatcgt gcgttccttt ttctaccaaa
     3361 tgcccttcat ggagaactaa aatttgatca catgcttttg ctattggtaa atgatgcgca
     3421 ataaaaataa tcgttttttc ttttaaattg aggagataat tcataattgc atgttctaac
     3481 aaggtatcta atccactagt tgcttcatct aaaattaata tgtccggatt ttttaataaa
     3541 gcccgtgcta aggctaatcg ttgtcgttgc cctcctgaaa gattatttcc accttcttcc
     3601 aacaatgtct cgtaacgtaa tggctgttga ttaataaatt catttagttg gactatctca
     3661 caaacttcta ttatttgttc gaaacttggt gggctatcta aaccgaatat aagattctca
     3721 atgattgttc cactaaagaa aaatgactct tgaggcaaat atgtcactcg atcgcgtaat
     3781 atctgaaaag gaatgtctaa gtgattaatg tttccataat ggatcgatcc ttctgatgct
     3841 tcataaaaac gaacaaataa ctttgcaaga gttgattttc cagaaccact aactccaact
     3901 atagccgttt tactgtgcga aggaattgta catgaaattt cgttcaacgt tggggccttc
     3961 atgctatacg agaatgaaac gttttttaca gtgatatctt gttgaaataa ttgttcagaa
     4021 agcatatatt caaaagactg gttcgatttt tctgaatcaa tcgcgaaaat ttcatttaaa
     4081 cgattatttg caacttgagc cgtttgcatt ttcacttgta aattaataat gttttgtaaa
     4141 ggatctgtca aaaatactag taaagcattg tatgtgataa gttgacctag actaattcta
     4201 ttatccataa caaaataaga acctagccat aaaattaatg cactgctaat taattgaatc
     4261 aaatgtttaa ttccttgttg aatattatca agcgtaaccg tacgaaatga ttttttcatt
     4321 aatttaacaa attcatggtc tatacggtta tatactttct cttcaccgct atatgcttta
     4381 attgtttcta ttcctttaag actttctatg atactagaat ttacagctgc accggctgcc
     4441 atttcttccc tgttcgattt ttcatagctt ttcacgaaag cgaatattgc aactacataa
     4501 aaaggcaaag agcatagagt gattaaaaac aacgtactat tctgaatagc taacgtaacc
     4561 ccaacaagca acaccatacc aatatctaaa aatattgata acgtagcact tgctaaagca
     4621 tcaataatct tactggcatc aagaaaacga gaaataattt cccctgattt tctcgtagca
     4681 aaaaaattca ttggtaaagt taacacatgt ttgaaatact gaagcataat tgccatactc
     4741 attcgctgtc ctaaaataat aagaagataa ctacgactat attcaaacaa tacccgaaat
     4801 aaatagacag cgatcaagcc aatggaaatt atatttaaag ttgaacgtgc ttgattaggg
     4861 atgaaaaaat caagaatacc ttgaaaataa tatgagcttc ctatgccaaa aaacgtaatg
     4921 aaaatcgatg ctaaaacgat gtggaatact aatccttttt gtttccaaat tataggcaaa
     4981 aaagaactta atccagctac tttttcttta ataggaatat aagtttctct aggcataggt
     5041 aataaaagaa tacctgtcca ttctttttga aacgcttcaa tcgtttttct attttttcca
     5101 ttagcgggat ctgcaatcca tagtacgttt cccttcgttt tataaattac aacgtagtgc
     5161 atatagctgt tatcaaccaa tatatgtgca attaatggta agataagatc tctttctttc
     5221 cagatctcac tatctgcttg aatagccata caatcaaaag acaatttttc aaatgtcttt
     5281 ttcaatccaa atgctgaagt accctctaaa tcagtcccag ataggtcccg taatttatga
     5341 atcgggatat cagttttaaa atattttaaa atcatcgaaa ggcaagcaac accacaatct
     5401 ttttcatctt gttgctgcac aaaaggaaat gattttatca cttatctaac acaactcctt
     5461 ctaaaaaatt tttatagaaa aattctcaat atagctttaa tacagtgaaa atattgtctc
     5521 ataattccac aaaaactttg aaaacagttc ttgaacacta actttttcat tcctgaatca
     5581 aatttatttt ggaaatttta ctattcaaga ataaacttga taagtttcag gaagattttt
     5641 actgttaatc aaatacgtat gttacagtag caccgtaaaa aatattattt aaagaagcgt
     5701 gtttaaacat gcaaaatgta aaggaactaa gtattgtgga aatgcacaaa ctgttggtgg
     5761 aggcaataat gcttggggaa aattggggca agttgtaggt ggacttacca caggggcagt
     5821 aggtggtgca ggtttaggaa cagcaatttg cggaccagct tgcggtgttg ttggagggtt
     5881 atatggagct gtcgctggag gtgctgcagc tggctgggat gctcgaaaaa aataaaaaag
     5941 gagaataagt agtatgcata ttaaaaacac aaaaacaaca tttatattat cttcagaaga
     6001 gttgaaaaat attcaaggtg ggagtgccgt gggagtttta ggcactactt tttctggggc
     6061 aacagcagga gtaaaacttt gttcagccgg aggaccttat gcaatagctg cttgtggtgt
     6121 aggtggagct ttattaggtg ctggattcag catgtggacc ggtgcgtaaa caattaaaaa
     6181 atatcaaacc tcaagattgg tttgtgtcga ttacgaacat aggtatgttc tttatctttt
     6241 caaaagtttg gaatggacag aactttttac taaagcttct tgtgctagtt tttataatta
     6301 tattaaattt ttatctttta ttaaagtcag ataaaaattc tttggaaatg taatggtaga
     6361 gatattt
//
LOCUS       DACDSY010000146         6153 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDSY010000146 DACDSY010000000
VERSION     DACDSY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.148"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..170)
                     /locus_tag="IWK44_002461"
     CDS             complement(<1..170)
                     /locus_tag="IWK44_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9550932.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFIT"
     gene            complement(164..565)
                     /locus_tag="IWK44_002462"
     CDS             complement(164..565)
                     /locus_tag="IWK44_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9550933.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(737..2545)
                     /gene="uidA"
                     /locus_tag="IWK44_002463"
     CDS             complement(737..2545)
                     /gene="uidA"
                     /locus_tag="IWK44_002463"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP9550934.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(2573..4339)
                     /locus_tag="IWK44_002464"
     CDS             complement(2573..4339)
                     /locus_tag="IWK44_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP9550935.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            complement(4352..5146)
                     /locus_tag="IWK44_002465"
     CDS             complement(4352..5146)
                     /locus_tag="IWK44_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9550936.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            complement(5157..>6153)
                     /locus_tag="IWK44_002466"
     CDS             complement(5157..>6153)
                     /locus_tag="IWK44_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9550937.1"
                     /translation="SINIPYGSMASAITSVAEERTALSTFRSIGATLAGMFIGVLTPI
                     FIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKSDESQLKVSLLK
                     SLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIVLMNFINPILML
                     VLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLVLTSVGYMGLGI
                     FNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVGGWTLSIIGYDS
                     LAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENYNLLNQKHN"
BASE COUNT         1946 a         1208 c          773 g         2226 t
ORIGIN      
        1 gtaataaatt gccccgcctc attaaatcca tttgtcaaac ataacttagc tatataacta
       61 tgataatgtt tgagtatcat cttgattgaa attggatctt cttctagagc agattggata
      121 atggatagag gaactaatgg atactgccgt tttttggggt tgtctaccat aagtcatacc
      181 ccatttttaa ttgttcaact gccttctttc gttgatacca gataccactt gtactcatat
      241 tgaggatttt accgatttca gtatcattaa aacctgcaaa ataataaagt aggataacct
      301 ttcttcttat ttccgataaa tgatcaatgg ctgccgataa cttttgatct gaaattaata
      361 tttccattcc aaataagaaa tagacatctt gttcatcaaa atgagtttgg gagtacttac
      421 cttcatttga attcgtcaat ggttcctcat tatctgttat gttaatgttt cgttttcgaa
      481 tagacttagt ttcgtttcgt aatacttttt tacagtaggc atcaaattga tgttgtatag
      541 atttctcatt gtaattttgt tgcatgataa atatgctcct tttttcccct ttccactaat
      601 acaacaatca gaaagcgcct ttttttcgaa aaaactagaa aaaaatatcc ttctttaaac
      661 agaaaatctt tttataaaaa aaaggagttg gcaattaagc agccactcct tttttatatt
      721 ccttataaaa ggtaaattat tttttataac tgaaatttgg tattgatgac catcgtttct
      781 ttagatagtg agcaatcatt ttaggctttc tatctctagt aaagattcct ttcttatttc
      841 cttgcactct taaaatccct tgacttgttt caaaatctgc aaaattccac acttgttcac
      901 caacaaaatt gtcacattta tctattactt cgtgatttgc ttcgtaataa cccacttgat
      961 actcttctgt aaacattgtt ggtactgtat catgaagtcc agctactgta tctgctccat
     1021 attctgtaaa cattattggc gtatcgggac atctttccat ccatccatct agctcttttt
     1081 tcaacagata ttttgcatgt tttaggtctc cacctgaaac ataccaacca taatagcgat
     1141 ttaaacataa aacatcgaca atatctccta ctttacaaat atctggtgtt gactttaagt
     1201 aagtaacgat tgttacaggt ctcttctgag ggtcaagttc tttagtcaat tcaactaaag
     1261 gttcaaaata ttctttagcg ccttcagaat ccgaatctgg ttcatttgca atagaccaca
     1321 ttacgacact cggatggttc ttgtcgcgtt ctatcaattc cttaatcgcc attttgtgag
     1381 cattttctgt ccgtatttct ttccaagtat catgtttttc tacgttgtca tctaataaag
     1441 tagccatgaa attcaaatgt aatcctactg caggtacttc atcaataact acaatacctt
     1501 ctcgatctgc taagcgcata atttcttctg aataaggata atgtgaagtc ctaaatgaat
     1561 tagcaccgat ccatttcatt aaattaaaat ctttgatatt tactgcttca ttcaaaccac
     1621 gaccattaat atacgtgtcc tcatgcttcc caaatccctt aaagtaaaaa ggtttattat
     1681 taatcaaaaa cttcccattc gaaacctgaa tactacgaat gccaaattct tctgtgtacg
     1741 tatcaatcac tttattttcg ttaattaaac taactttcat actatataag taagcattta
     1801 gaggctccca aagtgttggg ttcttaagtt gaatcactcc ttctttcccc ttagcttctg
     1861 ctataagttg agcttcttcg tcatagattg ctattagaat ttctccggtc cattctgtat
     1921 cgatattaaa ttctacattt gcatctgaat caattatttt tgtattaata gtgacatcct
     1981 ttatatagtc tttcggagtt gtatacaatt taacgggtct attaatgcct gcataattga
     2041 aaaagtcaaa attaaccaaa ttttcttttt ttatgtctcc atcttcctgt atcgtttctt
     2101 tcaaaatccc tacaggtaaa gtgctatcgt caataacatt gtttacagcc actgtaattc
     2161 tgttacttcc atcttttata aactgattaa tttctgattc aaatggcgta aaccctcctt
     2221 tgtgttgatt aatcaatttt ccattcaaaa atacttttgc ttcatgtgtt gcagatccaa
     2281 aacgaagaac taatctttct tctaataatt ttttaggaag aatcacttct ttttcatacc
     2341 acacccatcc aacatgattt ctaatgttct ctgtttcaaa taaatcatta taagaagaag
     2401 gaactgaaat tggataactc tctttttcaa ccattggttc taaagcaagt tcttcttgta
     2461 agtcatcatt tgtatctaac ttaaatctcc aaagaccgct taaatcaatt acttgcctcg
     2521 tctctgtcat aatgggatat aacatgtaaa tcactcctat ataatttaaa tattaaataa
     2581 atgtatcctt taacccacaa taagcatcta ttggacttat tcccttaaat tcagaaaggc
     2641 ccattagttt gtccactaat atttctaaag tttcttgttt tccatcataa gtattgatat
     2701 aagtttttac ttgaggcaca tcagctaaat gaaacggaca ctgtacagat acaaaaattg
     2761 ttggaatttc atgaatataa aatggaatat ccggagtacc tttacttgct ggccaaacaa
     2821 ttctttgaac tgtacctggg ttaacattag ctatattaat aatcaagtca tatttttcag
     2881 ttaaatcttt gattggtctc ttagcagcat atattccagc tatattttta attttttctt
     2941 cttgcgataa tcctttcaat tcttcttcag gtgaaatgta tttttcaact tcaaatcctt
     3001 ttgctgacaa aatctgtttc aataaatcaa tggcctgagg tctatttcca gcaactactt
     3061 caccaaaccc accttttacc ccatgtacat ctaccagtaa aatcctttta tatttttctg
     3121 gtacaatagg ccaaatatct tgtttatctt taactagtgt aattgcttga tcagctacct
     3181 ctttaaaaat tgatttattt tcaattaaac caattttttc catagcttct tgtttcggta
     3241 acaaaatttt attcttttct tttttatgca aacctagttt agcctttaag cctaaaattc
     3301 ttcttaatgc atcacgtaaa cgctcttctg aaataattcc tttgtaataa ccttccatca
     3361 tataattaaa gtcttcatct ggatcattaa aaaataggaa tagatcacat ccagccgcaa
     3421 tagaagtggg caataattct gaccgtttca ttgccccagt cattcctaac atatgactag
     3481 catcagtaac tactaatcca ttaaacccga gttttcctct aagtaagtca gtgattaatt
     3541 cttttgataa agttgcagga agtaaatctt catctttcag atcaagatta aaatgttttg
     3601 aataagctgg catatggata tgacctgcca taattgaagg taatccagct tctattaagc
     3661 ctgagtatac ttttccaaaa gaattatccc attcttctgt acttaaacta ttaatactaa
     3721 aagatagatg ttgatctcgt tcgtcaatac cgtcaccagg gaaatgttta gctgcaggca
     3781 aaattccact ctcttgaata cctttcatat aagctaaact aagttctaaa actttatcag
     3841 ggctttgtcc aaaagatcga ctagaaatga taggatttct ccaatttcta ttaatatcta
     3901 caataggagc aaaactccag ttacatccaa tagccgatgc ttcaattcca gaaactctcc
     3961 ctaattcata agcatatttc gcatcatttg ttgcaccaat ttttacttct acaccaactt
     4021 cggtaccatc tgtacaagct ccgttaccac ctgcttctgt attagcagca attaataaag
     4081 gaattttgct ttctgtttgt aggatatagt tttgatcata tacctcatca gcagttcctg
     4141 gattatatcg tacagctcca atatggtact gattgaccat actacttaag taatttttat
     4201 cacgactaga tcccatatta acaaataatt gacctatttt ttcttctaaa gtcatagcat
     4261 caatcgtctc ttcaatccaa gaaatagcat cttcatctaa atgatatggt ttttttcgta
     4321 agtctacttt tatagtcatt ttctttctcc tctacttatt agtaagattt ttccaaaaag
     4381 tatctatata tttttgttca atatctatat actctcccga taaacattta aataaaatat
     4441 cagttcttaa gttttcccat gattcttttt ctttattaac caaaatagga taaaagaaaa
     4501 ctttattctt atttgcagct tctagatcac caggagaatc accaatcatt aatatcttat
     4561 ccaaatcaca accattttca attacacttc gaataatgtc ttcttttttt cctttatctt
     4621 gacaacacag ttcatcaact aaggataata attcatgtct ttcccattct tcttgaatag
     4681 cttccttatt agctgagctt acaataatta tcttcccata ttttttcaga gcttttatag
     4741 tatctctagc acttaaaaag ggtttatcta atcctttagc ttgactaatc tgtcgattga
     4801 cttcttccga ccattttaaa gcttttaata aatcctctga ctgagagtat tctatttccc
     4861 tcttcaaaga tggattagac aattcttttg ttacagatac ccagtgcttt aatcgattaa
     4921 tcgatggtac atcatttttt tctaatgttt taactaaacc taaaaatctg tttattccac
     4981 gagtctctga atataaatta atattattcc aatcctgtag gaatttttcc ctatgatgaa
     5041 catcaaaaat ctctgcagca attggcccga aaaagagatt gtgtttataa gtcattgtat
     5101 ccatgacaca accatctgaa tcaatacaaa atatccaact gttcatgttt tttctcctag
     5161 ttatgttttt gatttagcaa attataattt tctaatactt tttctttaga taatggatat
     5221 acgaaaaata aaatcaatcc tacaacaaag aaacaaactg ctggaatcaa cgttgaagag
     5281 ttatacagct tttgtaaaac ttctccactt tgtacttttg ctaacgaatc ataccctatt
     5341 atagaaagag tccaacctcc gacaccgcca gctaaagctt gtccaatttt tcgggcaaaa
     5401 gaatatactg cataaatagt tccatcttct ctttggccag atttcacttc ttgatcatcg
     5461 attacatcag taatattcgc ccaaatgact gtattaaaaa tacctaaccc catatatcca
     5521 acagaagtaa gaactaagaa tacatacata ttactaggct ttatcaaaaa taaaagtaaa
     5581 tatacaatag cagagaagaa catccctact gacgccaatt cttttttacc gtatttttta
     5641 ctgaaaatta aacttacagg tgaaacaagt accaacatca aaataggatt gataaagttc
     5701 attaaaacta ttccctgtgc attattataa taatctggaa atacataatt gttcatactc
     5761 attgttaaaa gctgagcaag caacaaaaaa atagctgcag ctattataga aagcaacgct
     5821 ctatttttag ctaatccttt taaagatttc aacaatgaaa cttttaactg cgattcatca
     5881 cttttaaccc gttctacagt taatttataa catattacgt aacaaataac tgcacacact
     5941 gaaaatactc ctgccacaat tgtaaaagtt gatccaccac gtacaatttg agctccagaa
     6001 ctatctcttg tataaataaa aattggtgtt aacacgccaa tgaacatacc tgctaaggta
     6061 gctcctatac tacgaaatgt tgataacgct gtcctttctt ctgcaacaga agtgatagct
     6121 gatgccattg aaccgtaagg aatattaata gat
//
LOCUS       DACDSY010000147         6146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDSY010000147 DACDSY010000000
VERSION     DACDSY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.149"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            50..583
                     /locus_tag="IWK44_002467"
     CDS             50..583
                     /locus_tag="IWK44_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9550938.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            1218..1442
                     /locus_tag="IWK44_002468"
     CDS             1218..1442
                     /locus_tag="IWK44_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP9550939.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            1462..1749
                     /locus_tag="IWK44_002469"
     CDS             1462..1749
                     /locus_tag="IWK44_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9550940.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            2199..2579
                     /locus_tag="IWK44_002470"
     CDS             2199..2579
                     /locus_tag="IWK44_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9550941.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            2561..3475
                     /locus_tag="IWK44_002471"
     CDS             2561..3475
                     /locus_tag="IWK44_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9550942.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            3435..3713
                     /locus_tag="IWK44_002472"
     CDS             3435..3713
                     /locus_tag="IWK44_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550943.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            3720..4409
                     /locus_tag="IWK44_002473"
     CDS             3720..4409
                     /locus_tag="IWK44_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550944.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4530..4616)
                     /locus_tag="IWK44_002474"
     CDS             complement(4530..4616)
                     /locus_tag="IWK44_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9550945.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            5253..>6146
                     /locus_tag="IWK44_002475"
     CDS             5253..>6146
                     /locus_tag="IWK44_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9550946.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALS"
BASE COUNT         2317 a         1040 c         1153 g         1636 t
ORIGIN      
        1 tatctgattt agataattta ttccagtagc aacctaggag gcaaccagta tgcaaccaca
       61 agaaaccttt acaataaagg aattgtcaga actttttaag atgagtaggc aagcaatatc
      121 gaaacatatc caaaaattag attcttccat gatagctaaa aatgagcgtg gttataaggt
      181 tgtattgcgt tctggagtat tgcaacttgc tcgcaactta ggcaaccaaa tgttgctaga
      241 taggttgcac gagtacgaac aagacaacga taaaaatgtt aatataccaa gcaaaaacgt
      301 ggttacaaac aagttgctag atcagttgcg tgaagaaaat gaatttttac gagaacaact
      361 tgccctcaga gagcaaaata attctcatat acaaaaacta ctcgaccagc aacaacaact
      421 caccctacaa gccaacaaac aaatcgaaaa actgcaagaa aaactacagt tgacttatac
      481 agaagaagct ccagagaatg actcaacgac tctttctgaa gcaacagaaa caatagacaa
      541 acaaattgat actgaaaaaa aatggtggca actttggaaa taactggagg gaacattcat
      601 gacaagaaaa aatcttctat tcggttctat tttactagga attctaggat tcagtccgat
      661 gttattgtca gtgatcaata aagggaataa acatggtgaa ttgaaattga atccaaatta
      721 ttacaacagt cctaacgaag aattgtttgt aaaagaaaca gataaataat aaaaaagact
      781 gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      841 tatgaagcgc taccaaaaat ttatcatatt ttattaaaaa taacaaccca gtaaaaaatg
      901 actggtaaaa attcacaaag aaacggaaag aagcaaccgc agaaaatcaa gcttaaaaag
      961 agcttttgac ctagaccaat cgaaaatcac aaagtgagcg cctgtatcta ttgctacagg
     1021 cgctacttgg catagtgtac attatgtcta ctagctgttt aaataactga tcttataatt
     1081 ttatttaaac gattatgaat tcaaaactac tttttatgac gttaatcatt gttatataat
     1141 aaaatgttat ataataaaat tactaagatt atttaatata acaagcaatt ttaaaaaaac
     1201 gaaaaggaaa aacaatcatg aaaaagaaag tattaaaaca ttgtgttatt ctaggaatat
     1261 taggaacttg tctagctggc atcggtacag gaataaaagt tgatgcagct acttactatg
     1321 gaaatggtct ttattgtaac aaagaaaaat gttgggtaga ttggaatcaa gctaaaggag
     1381 aaattggaaa aattattgtt aatggttggg ttaatcatgg tccatgggca cctagaaggt
     1441 agtggtaggg aggttattat aatggatttt actaaagaag aaaaactttt aaatgcaatt
     1501 agtaaagtat acaatgaagc aactatagat gactatcctg acttaaaaga aaagctcttt
     1561 ctttattcta aagaaatcag tgagggaaaa agtgttggtg aagttagtat gaaattaagt
     1621 agttttcttg gaagatatat tttaaaacat aaatttggat tacctaaatc tttaatagaa
     1681 ttacaagaaa ttgttagtaa ggaatctcaa gtatatagag gatgggcttc tattggtatt
     1741 tggagttaat tctttggaat aaaatgaaaa tgggactgaa gaatcaattc tgattcatca
     1801 gtcccataaa ttatagagcc gaaaaattat catatttata gatttattaa tagtaaatgg
     1861 cataagaatc tttgaacaac gaaaaaaatg tagctctacc taattattga tttgcaacaa
     1921 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagtcttt
     1981 tttgtattgt ggagagtggg ggggaggggg attcaagggg gagcttgcgc ccctttgaca
     2041 agcggttatt tgcaccaaat acgtaggtat ttggctagca aatatagagc ttgccaagcc
     2101 ctttagtgag ctttagtgag ctttacttat tgtacctttt taggtacaat ggtattgcga
     2161 acgatacgtc cgtaggacgt actgaaaggg tgggacttat gagcgaacaa aatcaatatt
     2221 tggctagcaa tcattctaaa aaatatacct atcgatccga gccaaaacaa atcagttttc
     2281 gagtgagcga atccgaattt gcaaagctga agcagtcagc tgaagctttg caaatgagtg
     2341 tgccggcttt cgtgaaagcc aaggcacaaa acgcgcgtct ggtgacaccg aaagtggcgc
     2401 cagacattgc tcaggcaatc gcgcgcgatt tagccaaagc tggcgggaat atcaatcaaa
     2461 ttgccaagtg gtgcaacaca caccaacacg acgttgatcc tggcgacgcc caacgcttat
     2521 ccgaaaacct aaaaatcatg caaaaggagc tacaaaagat atggcaacag ttaaagtaag
     2581 ccgaaccact tcttgtagcc gagcgatcaa ttacgccgaa ccacgagcaa ccgtcaaaac
     2641 agggataaac tgtgacattg attatgccaa aagcgaaatg aaacaaattc gcatgctgta
     2701 tggaaaagac gatcgtgtac aagcacactt attgatccag tcttttcgac caggagaaat
     2761 taccgctgaa aaagccaatc aactaggggc agagtatgcg aaaaaaatag cccctgacca
     2821 ccaaatcgcg atttacacac atacagacaa agaccatatt cataaccaca ttgtcattaa
     2881 ctccgtcaat ctggaaactg ggaacaaatt tcaagcccat ggtcaagcat ttttaaacaa
     2941 atgttatgac ctaaatgacg agatttgtct tgaacatgac ttaacaatca ctgaacgagg
     3001 gaagaaaccc gaaaaacgaa ccatgtctga aatcaaatta aaagaaaaaa atgaacctgt
     3061 ttggaaagac gaaattcggt ttgcgattga tcaaacaatg aaaaacaaaa aaactcgaac
     3121 gtataaccaa ttttgtgatt ctcttaaaac attcggcatt cattgcttga atcgtgggaa
     3181 aaattttact tacgaattga tccacaaaaa gaaaaaagta cgatccaaca aactaggaaa
     3241 ggattacgaa aaggagacga ttttacatga gctggacaga cgagaacgag cctacaatca
     3301 acaacgaacc aacgagctac gaagagctcg agctagcatt cgcgaaactg caaaagaagt
     3361 acgaagagaa caacgaactc ttacgactga acaaccggaa attagaccga ctcgaagaaa
     3421 ccatgaccaa cacgttgaaa gaggagcaga ctaccaacaa gaattatcgt tctaaaacgt
     3481 tacaagacat agaaaatgaa ataaagaaac aacgtgcttt tcttgcacct tataccaaag
     3541 aaagaataca aactacaatt gattcacaag tcaaaaaatt ccaccatgcg ctattaaacc
     3601 agcgaaataa agaacagaaa aaagtaatta tgcagcgatc aatggatatt cttgccccct
     3661 tatgttgctt aatcatgaca ctgttagcct tttggctggt atggggagaa taaatagaaa
     3721 tggcattcta tttaccctat ctactcattt ttgccagtat tttaggaatt atttggctta
     3781 gctataaggc ttatcaaaca agatattcat taaaaggctc taaaattcga tttaagaggt
     3841 ttttattgcc aggggttata tttacattat caatcgtttt aagtggttta ttcggcgttt
     3901 tagagggaaa taaacgtatt tcaagaagta ttttattagg ggatatcact caaaagtatg
     3961 aagcagcatg tgataaaaag aaaaaggagc aagtgttaac acaaaaaatg gaagagttta
     4021 ccacttgtta tgaggaaatg aacgatattt ttgtgaatca agaaaaacgt ttgacggata
     4081 aaaacatgga aaaactcact cgtctttatc aaaacttgcc agaaaaacag caaaaagagg
     4141 ttcaagacaa ctatgagcga accaaaaaag acgtgcaata ttttaaagat acgcagacag
     4201 aagaagcttg ttataattta ttcagtgata caatcccttt ttcaactagt gaacaggaac
     4261 gaaaagaacg gcaacaatca gttacctacg aacgttataa agcactgctt cagcaagcaa
     4321 cgaacataca aaatccaacg aaaaaagaaa cggcattaaa ttatctaaaa agtgtcaagg
     4381 aatggttaga tcagcaacag caaaattgaa tttgatcggc aatcatgtta aactaaacat
     4441 gcaaatgatc aaacattaga tacacgaaag ccccagagag gtcggattct ctggggcttt
     4501 ttttcaggtt gatgtcgcct ttattgattt tacttcttgt ttcggttatt caaccaataa
     4561 ctgaataaag agacacaaac ttctataaga agggggcaaa tgaacaattc aaacattaga
     4621 tacacctcct tccagtcgca agactgtcgg ataggcgaca agttattata tcttaaaaaa
     4681 ttaaacttgt gtggtgtttt taagtatcgc ttattttagg gttgttaggg cttgccctga
     4741 ccgcctgtaa ggcgctttaa agctagataa ggatttttat ccaagactgt cttaaaacgg
     4801 cttagataag cattcagaac cgctcaaagg cctctttacc aacatgtttt tagcaaagga
     4861 gcctttaggc gactgcgcag tacaatgtga gagcgtagcg atcacacctt tttggcgacc
     4921 gtaggaagca aggttttgga ttggcagttt tgaaaaatgc gcgaacctgt ttaaagaaaa
     4981 aagaagtggt ttttaggcgg gaaaattagc ccccctcttt ttatatctct ttttaaacct
     5041 ctttttaaac ctctttaccg taagctgtgt ccttactcta ccaatggata cagaatgtta
     5101 tgaaggacat tttgtctgtt tatgaaggac attttgtctg tttatgaagg acattttgtc
     5161 tgtttatgaa ggacattttg tctgtttgaa cgtcaaatat gaatgtcttg acatttaaaa
     5221 aatgtcattc ataattggat tgaggtgatt atatggctaa tgagattgta aaataccacc
     5281 atgaattaaa tacaattcct ttaagaaaat ttacaccggt tgaaatgaat ttgttttttt
     5341 ccatcgtgtc tagaatgcgt gaaaaagggg ataagacagt tcgttttacg tttgaccaac
     5401 taaaagattt aagtaactac aaagcaactg caaatgtacg atttgtagaa gatttaaaag
     5461 caacttatga aaaaatctta tctttacgct ttggtcgtag aagtgagtct gggctttctt
     5521 acgatatgtt tgtaatgttt acagaattta aaatcaacgg ggacgtagaa aatccttacg
     5581 tagatatacg aatttacgaa aaagcactgc ctttgttaaa taatttagac gagtgggtac
     5641 gctattcttt acaacaattc aacgagctac aaagtggcta tgcaaagact atgtttcgtc
     5701 tgttaaagca atacagaacc aaaggtttcg catatttctc caaagaagac tttcttgaat
     5761 tattagatat tcctaaaagt tataagcaac ctgatataga taaacgagtt ataaaaccta
     5821 tacgtcaaga actaactgca atcttcaaag gactaacaat caaaaagaaa tacggaaaag
     5881 gacggggaaa acctgttatt ggctatcaat ttacgttcaa acctgagatc aaaaaagcag
     5941 acgactttaa caaacatgca ccaaccaaag aagaccaaaa taaagcattg gctttagctg
     6001 ataaacaaaa agaatacgaa gaacccgcta caacaattaa cgaaaaaatc aaacagaaga
     6061 aacgagaact tgaactcctc caacaaatca aacaactaga aaaagagaaa aaagctcttg
     6121 aaaaacaaac agagtccgca ctttcg
//
LOCUS       DACDSY010000148         6057 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDSY010000148 DACDSY010000000
VERSION     DACDSY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.150"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(441..1223)
                     /locus_tag="IWK44_002476"
     CDS             complement(441..1223)
                     /locus_tag="IWK44_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550947.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1288..2124)
                     /locus_tag="IWK44_002477"
     CDS             complement(1288..2124)
                     /locus_tag="IWK44_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9550948.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2142..3551)
                     /locus_tag="IWK44_002478"
     CDS             complement(2142..3551)
                     /locus_tag="IWK44_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9550949.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3565..5388)
                     /locus_tag="IWK44_002479"
     CDS             complement(3565..5388)
                     /locus_tag="IWK44_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9550950.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1884 a         1297 c          972 g         1904 t
ORIGIN      
        1 aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg aaaggagtct
       61 aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt ctacatagtt
      121 atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt cataaaaaaa
      181 cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca ctaaaatcac
      241 tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc tccgtttcat
      301 ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc ccagtagctt
      361 atcctaagct actggagatg tataagtacg gtcatataac aacggggtag ggtaaataaa
      421 tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt gtaatcagtt
      481 acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag taaccgaagt
      541 ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag attagctaat
      601 aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat cgtgattcct
      661 ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc gatgaatgaa
      721 tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact cttagatgaa
      781 tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt ctcaccggaa
      841 actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt taaaattccc
      901 acgcttttac taataaattc actataacca gttctttcaa tagagacttc aatgtatttc
      961 acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt tacatctaca
     1021 taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt actgtctgat
     1081 atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc ttctggcaag
     1141 ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac attcacctcc
     1201 gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac aagtctttat
     1261 atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca gtgttagata
     1321 aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact ccttgatttt
     1381 caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca aaaaatcatc
     1441 cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta aatgcgtcac
     1501 aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca gatatcctag
     1561 catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca tatttgcttg
     1621 ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt ctggcaactg
     1681 aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa attcttttgg
     1741 gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt tttgactgcg
     1801 ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat tcagcttaaa
     1861 gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta gatcatccgg
     1921 agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg aatctaattt
     1981 aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt gaaatccaat
     2041 ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa ttaaactgtt
     2101 gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa gccatttctt
     2161 gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa ttctttaaga
     2221 ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc ttgatgggta
     2281 ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc atctgttaaa
     2341 gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg gtcattaatt
     2401 cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat taaaataggt
     2461 aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg gtcaactacc
     2521 cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac accttcttct
     2581 cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc atttttgctg
     2641 gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt accggaggat
     2701 aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt caaatagttt
     2761 tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca gcctctcaat
     2821 gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg atacatagcc
     2881 gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata caagtgaatt
     2941 actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt ttttgaaggc
     3001 agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt gttttgctca
     3061 ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac ctgtacatag
     3121 tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc tagaaaataa
     3181 ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag gcattcatca
     3241 attacttgat gataaaaaga aattcccttt tgattggttt tattattttc aatatccata
     3301 attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt aaacagctgt
     3361 atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc taccccttct
     3421 gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata ggcaccctct
     3481 gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc tgaaagctgt
     3541 ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt gttcctttaa
     3601 gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta ccacaatcac
     3661 tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta ataattgtcc
     3721 tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt ttaaattcac
     3781 ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta gaccaatcgc
     3841 atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa tgctgttttc
     3901 ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag catcgttcac
     3961 ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg cttcggctat
     4021 tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag caatgataaa
     4081 ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca ttggattttc
     4141 tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc tgactaaacc
     4201 gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa ctaatggttt
     4261 tttcagcttc aaggttactc catagagtga aggctcagta attcccatta aagcagtgat
     4321 cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg ctacagcaaa
     4381 ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga tcatttcaga
     4441 acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg tacccgtaac
     4501 caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg ctttgccgtt
     4561 aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg gtccaatgac
     4621 aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa agaatttaat
     4681 aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc aaacagtaaa
     4741 gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc ctagaaaaga
     4801 aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa tgccagcaac
     4861 agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg ctaatagtac
     4921 tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag tttgactact
     4981 gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca tcccggcacc
     5041 tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca atcttgaaat
     5101 aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca cttccttttg
     5161 aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt gtccaccgct
     5221 atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg ctttcctttt
     5281 atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca cattttcttt
     5341 tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta ttctctctcc
     5401 ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa gtgttagttt
     5461 gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc tgtaatccaa
     5521 taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct tttcgtttga
     5581 ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc gtatacttac
     5641 tgctaaaata agtatatcta atattaacta taccagtaaa accattccta cataaccagc
     5701 gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac acaacttcaa
     5761 caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa taaacagcat
     5821 tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca gtaagcaata
     5881 gccaatttca atcattccga gatataaagg tatcgataga atgatttggg ctagtattct
     5941 taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt agtaagtatt
     6001 tcaattccac caattatcaa taaaaatacc aaacatatcc ataataattt tttcttt
//
LOCUS       DACDSY010000149         6002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDSY010000149 DACDSY010000000
VERSION     DACDSY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.151"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IWK44_002480"
     CDS             <1..395
                     /locus_tag="IWK44_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550951.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="IWK44_002481"
     CDS             398..1228
                     /locus_tag="IWK44_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9550952.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="IWK44_002482"
     CDS             1244..2095
                     /locus_tag="IWK44_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9550953.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="IWK44_002483"
     CDS             2092..2451
                     /locus_tag="IWK44_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP9550954.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="IWK44_002484"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="IWK44_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="IWK44_002485"
     CDS             2624..2929
                     /locus_tag="IWK44_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550955.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="IWK44_002486"
     CDS             2929..3240
                     /locus_tag="IWK44_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550956.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3407..3823
                     /locus_tag="IWK44_002487"
     CDS             3407..3823
                     /locus_tag="IWK44_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP9550957.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="IWK44_002488"
     CDS             3823..4491
                     /locus_tag="IWK44_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9550958.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="IWK44_002489"
     CDS             4488..4865
                     /locus_tag="IWK44_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550959.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="IWK44_002490"
     CDS             4862..5161
                     /locus_tag="IWK44_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550960.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5403..5819
                     /locus_tag="IWK44_002491"
     CDS             5403..5819
                     /locus_tag="IWK44_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP9550961.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DACDSY010000150         5903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDSY010000150 DACDSY010000000
VERSION     DACDSY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.153"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..985)
                     /locus_tag="IWK44_002492"
     CDS             complement(<1..985)
                     /locus_tag="IWK44_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HAP9550962.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="IWK44_002493"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="IWK44_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP9550963.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="IWK44_002494"
     CDS             1810..2964
                     /locus_tag="IWK44_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9550964.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="IWK44_002495"
     CDS             3179..4147
                     /locus_tag="IWK44_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP9550965.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="IWK44_002496"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="IWK44_002496"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP9550966.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..5785
                     /locus_tag="IWK44_002497"
     CDS             5177..5785
                     /locus_tag="IWK44_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP9550967.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1782 a         1149 c         1335 g         1637 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc ggcagtgcca ttgatcttac gctttatcga ttagacacgg gtgagcttgt
     5581 accaatgggg agccgatttg attttatgga tgaacgctct catcatgcgg caaatggaat
     5641 atcatgcaat gaagcgcaaa atcgcagacg tttgcgctcc atcatggaaa acagtgggtt
     5701 tgaagcatat agcctcgaat ggtggcacta tgtattaaga gacgaaccat accccaatag
     5761 ctattttgat ttccccgtta aataaacttt taaccgttgc acggacaaac tatataagct
     5821 aactctttcg gcaggaaacc cgacgtatgt aactggttct tagggaattt atatatagta
     5881 gatagtattg aagatgtaag gca
//
LOCUS       DACDSY010000151         5846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDSY010000151 DACDSY010000000
VERSION     DACDSY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.154"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..3127)
                     /locus_tag="IWK44_002498"
     CDS             complement(<1..3127)
                     /locus_tag="IWK44_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP9550968.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELI"
     gene            complement(3141..4358)
                     /locus_tag="IWK44_002499"
     CDS             complement(3141..4358)
                     /locus_tag="IWK44_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9550969.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4348..>5846)
                     /locus_tag="IWK44_002500"
     CDS             complement(4348..>5846)
                     /locus_tag="IWK44_002500"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9550970.1"
                     /translation="FYKYLSDRLLEQVVLLADESLEEYDTVSKQTMLYRELLSDEESK
                     EDLIATIVDILGYSISPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDD
                     VDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKA
                     GEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQ
                     ELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWS
                     ADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGV
                     IRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQ
                     NKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMV
                     HVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT         1591 a         1242 c          987 g         2026 t
ORIGIN      
        1 gaatcaactc atcatctaac attttacgcc atgctcgctt catttcttgt gggtatttaa
       61 atggtttcgc ttctggatgt ttggctttat atccttcgta atccttactg ttgataattg
      121 ctttcatatt aggaattgga tcttcacctg gcatatattg cagattagaa gcatgtaatt
      181 ctttctcaga aacaaaccac tcattggaaa aggcatgaat caaattatca cttgcttgtg
      241 taaaatagcg acgtttaatg acaaagatat cttcattact atctaaagtc cccacatcaa
      301 atgcatttaa aatttgtcgg taaacttctt tgactccttc tgttttattc aatttagcca
      361 aagctttttc aatttcttct cgtgcatctg aactattcgc agcataagtt cctagtaatt
      421 gatcaatata agtagaatca atatcataca acttagtggt attttcacta taaaattcga
      481 tcgttgaaaa atcttctccc tctttttctg gttcttcctc catcaatgaa cccttcacgg
      541 tctcgtacac gccaatttgt tcttccaaaa gttgaatttg attgttcaaa gttgaagatt
      601 gcaccatgtc atcattgtaa tcatcataag tcactaacga ttcatacgca cgattcaatt
      661 cttgaaatgc tttgacgtac tcgatacgga tttcaagtgg cgtatgttca tcaatggatt
      721 ccatggatga aacctctttt aacttaccat atgcttgctt gaatcttttt ttggattctt
      781 ggtaacttgg ataaatcaaa cccgattctt ctttagcttc tgaatacagt tttgtcgcat
      841 cctcgacatt cttctccatt gtcgctggct ttcggaaagt aacaattagt ccttttgttt
      901 tttcaggata cgtacggttc gtacgtgaaa aggcttggat caatcctgca tactctagat
      961 tacgatccac aaatagggtt tgaatcgtag gggcgtcaaa acctgtcaat agacgatcca
     1021 ctacgattac taaatcgatt tgtttcccaa attgtttgaa ttccgctctt tttcgtgcca
     1081 aacgattatt gatgtctccg ttataacgct caatgtctgc tagtgaccag gcagtatcat
     1141 aatactgatt atattcctca ataatttttt gcatttcttc ttgttgggca cttgaatctc
     1201 ggctattttc ttctaatgaa taggtaatag cgattcgtgg gaaatcagga tcctccatcg
     1261 ttcgtccttc acggataggc tgattgggaa actcttttat taaccaatca gattctttcg
     1321 tcatctcttt gatggcccga taatactttt tggccatatc aatggaactc gtggttaaga
     1381 tcgcagactt cgttggacgg ccatttcgaa aatcaaattt tgtataggca ttgtctggac
     1441 ggaacatttt atgaatgact ttttggatat gttcctctgc ttcaaaaaca gtagggtcca
     1501 aataactttc ttttttgaca gactccattt gatcgatcat acgattgatc tgttccggtg
     1561 aatactctgc atatgtttct acttctttta atttccgata gattttattc tctaatgaag
     1621 tcggctccac ggtgttttca tgctctactt gaaatcctaa aaccgagcca tcttctaatg
     1681 cattcttgat ggtataggtg tgcaagacct ctccatactg atcatgggtc gtacgtgcca
     1741 attgcccttt ggcttgtttc cgattctcag ggaaaatcgg tgtacctgta aagccaaacc
     1801 aagtggattt cggaaacatt tttttgattt cttccatatt ttcagcactt aacgctcgat
     1861 gacactcatc aactacaaac acgatatgtt gccccattaa tttttggaag cgattggttc
     1921 cttttttctc ttcctgtttc tttgcatatt tcaacgccgc atctagtttt tgacgggttg
     1981 taatgatcac aacattcgca ttggcatctg ccaacagagt ttcacttaac tctttggcac
     2041 ttcctgttcc cacgatcaag ctattcgctt tggcattacc agaagaaacc ccagtattaa
     2101 actccgaagc aaatttcgta aactctgtgg tggtctgatt atctaaatct ttgcggtcca
     2161 ctaacatgat cgttcgatcg atccccgatt ttcgtgctaa aagcttagta gatacaaaac
     2221 tagtcaacgt ctttcctgaa ccagtcgcat gccacacata ccctgattga tgtttatttg
     2281 cggcaataaa caaggcttga atcgcatgca cttgataagg atgtaacacc atgagggttt
     2341 tattgtcttg atcttcactg acaatcgtat aatccgcaat caagcgatgg gcatctggga
     2401 tatttagcac ttgtttgcaa aactcatata agttttctac ctttttatta tctttcgttc
     2461 gccaactaaa taaaaactta ggatgcaaat ccttcggtaa ggcatttgca aaataccgag
     2521 tcgtttgttc attggaaacc acaaacaact gtaaggtcga aaagatattg ttccgaaaca
     2581 tgccttcttc agcatatttt ttgatttgat taaacgcctg ataaaaacca tcttttgcag
     2641 ttacttgttt tagctcgatt tggataatcg gtaagccatt gatcaataag gtgacgtcaa
     2701 aacgtcgatc ccgcgcttct atgttacttc cacgttttgc aatttgatgg accacttcat
     2761 aagtagagat tcctccacca atgtcttgat tggaataaag gataagtgag acagaaccca
     2821 attgcggatt ttcccgttca atcgtgatcc gagccatccc attttcacct tttaaccatt
     2881 tcgcagcatc aaatggggtt ttcgtccgta gcaataattc ggttttgatc gtttcaaact
     2941 ctttatcggt caaaggagaa tcattcagtt ctgcttgatt atttgagatg attttttgac
     3001 gtaaattgct ccaaagatct tcttctgatt ttagatccgg acgatacgtc cactgattat
     3061 gtccttcccc gaggacacga atcaactgtt cttcgacttc cgcttcctca cggtgactaa
     3121 ttttcattct tcttccctcc ctaaacaaac atcttttgca agaagccttt tttcgtttcc
     3181 ttcagcaaat ctaacttacg ttgatgaaga gcgatcgtat catctagctg cttgaagaag
     3241 gcaccaattt tttgttgttc atttatatcc ggtggaaact gaaatgtcct ttgttctata
     3301 ccacctttag tcacatgtac cattgtcgtt ccgttagttg attctttaat tttctcttta
     3361 tcagtcaata gccaagtata tagatactgt ttgtccaatt ccttagataa aaatttaagt
     3421 ttccaaatat gataatggta gattacacgt tcttcattcc acatttcagg accgaagctt
     3481 gttgcccata aatataagag atctccttta ttagcatact tgtcttcctc taattctaag
     3541 tttgaataat accatctatc attagtatta aaattaccta ctcttaaaac cctatatttt
     3601 ccagaatcaa gtaattcttg ttgtttaaaa gctctaccat tcaaaaaaac taccacttcc
     3661 cccaacttcc gctcttccca atctcccgta aatcctggaa aacgaacttc tggcactttt
     3721 gctccgtttt tagggaacat tttttgtaag aatccttttt tcgtttcctt cagcaaatct
     3781 aattcgtgct ggtgaagcat gatggtgtga tcaagttgtt tgaaaaatga accgatcttg
     3841 atctgttcgt cataagttgg aagtgagata ggtgtctttt taccactatc aattgtatag
     3901 gttccgacgg ttccaatgcc tgcaacgtca cggatatact tctgaatttt gttatttatg
     3961 aacgagtagt aaaagaagtt tccgtcaatt ttatgctcat tcttgaacca aatgatgttt
     4021 ccatccttaa agtaaattgg gttatcatca gtgattaata aggggacacc tattgatccg
     4081 acaccagtta caagcaagtc tccttgagaa actttacctg aatttttcga atattcatta
     4141 tatttttctt ctgaaatgta aagataatcg gatggttcct cattttttgc agctgagaca
     4201 atatcacgag cacgtaaaaa tctaatacct gagttagtcc aatctgattg atgaattcgc
     4261 ttcacagaag taacatccat catatctcct aacttacgct cttcccaatc ttccgtaaaa
     4321 ccaggaaacc ggatttctgg tcgtgtatca ttgctcatgt ttgaacacct ccagcgcacc
     4381 ttgtaaccac gcttcatttt caggtgttgt ttgcagggaa gagatagctt ctaaaagttc
     4441 tttttctagc acttgctgtt cttgtcgtat cttcttgatg tcatttccga catgaaccat
     4501 atcaattggt tcttcttctt cgaaagtatc gacatagcgt gggatattta agttatagtc
     4561 attttctttg atttcatcaa aggttgccaa atgagcgtat ttctcgacat cttttctttc
     4621 agcgtaggtt tcgaggattt tttgaatgtt ttcttccgat aatttattct gatttttccc
     4681 tttaacaaat tcacgactag catcaatgaa taatacatct cgagtttgac gattcttctt
     4741 caatacaatc accgtcgttg gaatcgatgt accaaagaac aagttcgcag gcataccgat
     4801 cacagcataa atactgccgt cttctaataa tttttgacga atgacccctt ctgcagcacc
     4861 acggaataaa accccgtgtg gcaagacgat agccatagtc cctgtttcct tcaaatgata
     4921 gaaaccatga agaagaaacg caaaatctgc tttggatttt ggcgctaatt tcccgtagcg
     4981 attaaaccga gaatcatcta agaaggtcgt atctgctgac caattagccg aataaggtgg
     5041 attcatcact actgcatcaa atgtgtaagg ttcatctgtt ggccagtcct tattcaacgt
     5101 atccccattg cggagattca tttcttcggc atctacccca tgtaaaatca agttcatttt
     5161 tgctagattg tatgtcgttg tatttagttc ttgcccatga tatttaacgt tatctggatg
     5221 agttaagtag ttacgtacat ttaacatcaa ggaacctgaa cccatggttg ggtcaaagac
     5281 actaaagaat cgtcgttctt tttgatccaa cgtcacgatt tgtgccatca tatcagaaac
     5341 catatgaggc gtgtaaaatt ctccggcttt cttccctgct tctgaagcaa attgactgat
     5401 cagaaattca tacgcatccc cgatcacatc cccatcgtga cctaaaactt ccacgtcatt
     5461 caatttcttg ataacttcgg taatcgtcac atttcgttgt tgctcatctg ttcctagttt
     5521 ttttgattgg agatccacat catcaaataa cccattaaat tgattgtagg tagatgccaa
     5581 ttgcacaaag gctttgttta gatcgttcaa ttggaacgtt gcttgttttg cttgatccgc
     5641 taagacgttg aaaagatact ctggtgagat cgaatacccc aaaatatcaa cgattgtggc
     5701 aatcaggtct tcttttgatt cttcatctga aagtaattca cgatacaaca ttgtttgttt
     5761 gcttactgtg tcatattcct ctaaggattc atctgctaaa agaaccactt gttccaacaa
     5821 acggtcggat aaatatttat agaaaa
//
LOCUS       DACDSY010000152         5817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDSY010000152 DACDSY010000000
VERSION     DACDSY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.155"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..967
                     /locus_tag="IWK44_002501"
     CDS             <1..967
                     /locus_tag="IWK44_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9550971.1"
                     /translation="LHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFDKEL
                     TVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICLSNE
                     EGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEFDKL
                     NDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWINSNA
                     IHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADFDLE
                     SIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            949..1410
                     /locus_tag="IWK44_002502"
     CDS             949..1410
                     /locus_tag="IWK44_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9550972.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1460..1924
                     /locus_tag="IWK44_002503"
     CDS             1460..1924
                     /locus_tag="IWK44_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9550973.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            1938..2687
                     /locus_tag="IWK44_002504"
     CDS             1938..2687
                     /locus_tag="IWK44_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9550974.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2687..3517
                     /locus_tag="IWK44_002505"
     CDS             2687..3517
                     /locus_tag="IWK44_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9550975.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3517..4164
                     /locus_tag="IWK44_002506"
     CDS             3517..4164
                     /locus_tag="IWK44_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9550976.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4262..5038)
                     /locus_tag="IWK44_002507"
     CDS             complement(4262..5038)
                     /locus_tag="IWK44_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9550977.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5182..>5817
                     /locus_tag="IWK44_002508"
     CDS             5182..>5817
                     /locus_tag="IWK44_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9550978.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSM"
BASE COUNT         1972 a          841 c         1060 g         1944 t
ORIGIN      
        1 actacacaaa gaacagattg aaacagcaat ggcagcgtta aaggaaaaga caattgatca
       61 tgtatatttt acagcatgtg ggggttcaca agccgtttta atggctggtc aatatctatt
      121 tgataaagaa ttaactgtac cttgtcatgt ttatactgct aacgaattta actatgctat
      181 accaaaagga ttatctgaaa attctttagt tatcacttgt tcccattctg gcacaacacc
      241 agaaactgta gaagcaacaa aaaaagcaag agaatttgga gcaatgacta tttgtttatc
      301 taatgaagaa ggatctcctc tatgggaagc agctgaatat cctgttcact atgattgggg
      361 taaagatgtt gatgctagtg atttaaataa aggaattctt tatggtttat tatttaatat
      421 tttacttgtt ttagatggct ctaaaaaatg ggaaacttgc ttgaatgaat ttgataagtt
      481 aaatgatctt gttattgctg aaaagagcaa atttgaaata actgctaaag aatggggtaa
      541 aaaccaaaaa cgagataaaa ttatttacac aattggtagc ggtatcaatt acggagaaat
      601 ttactccaca gcagcttgct ggttcatgga aatgcaatgg attaattcta atgctattca
      661 ttctggggaa tttttccatg gtccttttga aatcactgat tatgatgtac cattcatctt
      721 agtgaaaagt attggagata caagattttt agatcaacgg gtagaagact tcactaaaaa
      781 attcagtaat aaattaatgg ttttagacca agctgatttt gacttagaat ctatagatag
      841 tgtagcgaaa gaatatattg cagctgtact tagtggcgtt gttattcgcc aaatggtaga
      901 agcgattgct tttgaaagag gccattcctt agatgttaga cgctatatgt ggcaaatgaa
      961 atattaatta ataggaggtt gcaaaagatg gcaaatcaat tatttcacat tgtgattaca
     1021 acacattcta ccctttgtga gggttattta aaggcaacaa aattaatttt aatgaatgac
     1081 gccacaggta ttagtgctgt tccatttaaa gaaaatacta gtccagatga ttttgaggag
     1141 agtatgaaag aaataattag tcagcatcat ttgcaacctc taattatttt aacggattta
     1201 attggaggaa cacctaataa tgtttctatt aaacagttga acaacaatag aattcaaatt
     1261 attgctggga ttaatttacc tcttttactg gagttattaa tggcccagtc agctggtaaa
     1321 tctatagaag aaatagattt taaggaatta ctgaataact ctcaaaaaag tatggttcat
     1381 attaattcgc ttttaaaaga tgtcagttaa ggtggcaatt tctattctat aaattttaaa
     1441 aattgaagga ggaaatagaa tgattaaatt agtgcgtgta gaccacagat tacttcatgg
     1501 acaagtaata ttttcttgga ctaaacagta tgatattaac catattattg tggctgataa
     1561 caaagttcca ggagatccaa tgtctgtgat ggctttgaca attgctaaac ctgctgattg
     1621 caaattagac attgttaaaa ttagtgatgt taaagggatt atggaaacat ccccaaatga
     1681 taattacatg attttaatta aaggacctaa ggaagctttt gaattagttc aaaatttacc
     1741 agaaattaaa gaaattaatt ttggcggtgt gtctaagaaa agcgagtcca aacaatatgg
     1801 aaaggcagtc tttttaactc ctgaagaatt agaagccaca aaaaaaattc ttgatcaaga
     1861 agtgaaaata tttgtgcaac aagtcccatc ttcaagtatt gaaaatgtag attttagtaa
     1921 gtaagggggg aaaataaatg ttattaaaag ccattttatt aggtttagtt ggtgtggtag
     1981 ctgttattga ctccaggtta attggtcgac aaaatattgg tagaccacta attttgagta
     2041 cacttgccgg acttgtgcta ggtgacttgc atacagggat tgttcttggg gcatctttag
     2101 aattaatttc tatgggattc gtctcaattg gggcggctgg acctcctaat atgcaacttg
     2161 gtagtattat tgccactgct tttgcaattt taactaatag tagtacagaa acagccttaa
     2221 caatagctat cccagtggct gtagctggtg aatttatgag tattatcatg cgcatgataa
     2281 ttgctcaatt tggacatgct gcagataaag ccattgaaaa taataaattt agaaaagctc
     2341 aaattattca tatttattgg tcatttattt ttaatgcttt tgtttacttt attccaatct
     2401 ttttaacagt atattttggt gctgacgttg tggcagattt agtagataaa attccagaaa
     2461 tgattacaga cgccttgact gtctctggaa atatgctttc cgcattaggg tttgcaatgc
     2521 tattaagcac aatgttgagt aaaaaattat atccttactt tatttttgga ttctttattg
     2581 ttgcctattc aggtctttca ctaattggcg tgacaatttt tgcagctctg attgcctttg
     2641 taatggatca agttaaatat ggaaaaagag aagaggcgca tggttaatgg aaaatagtac
     2701 agaaaaaaaa cgtttaatca caaaaaaaga tcttaataag atgtgtcttc gttcatggac
     2761 aatcatgtgt tcgtggaatt atgaaagaca aatgcatatg ggatttatgt atgggatggc
     2821 gccaatttta aataaagttt ataaaaatga tgaagagaaa aaaagagaag cttatactcg
     2881 tcatatggca ttttttaact gtacgcctca aatgacttca tttattatgg gactttgtgc
     2941 ttctatggaa gaacaaaatg ctcaagcaga agaaggaaac tttaatactg aatctatctc
     3001 catgattaaa accagtttaa tgggtccttt tgctggaatt ggtgattcat ttttccaagg
     3061 tactttaaga attattacat ttgggattgg attgtctttt gctaagcaag gtagtatttt
     3121 aggaccgatt ttagctgttg gcctttttgc tataccttct attttagctg ttttttacgg
     3181 cggtaaatta ggatatgagt ctggcaataa ataccttact aaattgtatc aagaaggtct
     3241 tatggatcga gtaatgcaat ttgcttctat cattggtttg gctgtagtag gtggaatggt
     3301 ggctagcatg gtgccaattg tcaccccttt aaaattcact acagggggaa cagaacttgt
     3361 tttacaagac ttacttgatt ccattattcc tggattatta ccacttgtat gtactttggg
     3421 aatatataaa ttgattaaga aaaatgttag tacaaatatg ctcttattag gttgtatttt
     3481 ggcagggttt gttttaagta taataggtat cttataatga ttaaggctct aatttttgat
     3541 atggacggag ttttaattga tagcgagatt atgtatcaaa agtgggtatt taatttttta
     3601 aaacaagaaa atttaagcta tcctttgtat tcttatcaaa aaaagattgg aactacagca
     3661 agtatttttg atgatataga aaagtacaat gagggatgta acagtttaga attgaaggga
     3721 aaatttacta aatattggaa atcgcaaaag attgattact tggatatttt tcctaatata
     3781 ctaattgaac agcttgaatg gttaaaaaag aaaaaaataa aaatggcaat tgcttcttcc
     3841 agtccaatgg atactataaa tgaagttgtt accacttgta aaatagagtc atattttgat
     3901 tgtttaatta gcggaagaga tttgccagaa agtaaaccta atcctactat attccttaaa
     3961 gcttcagaac agctcagaat tccagttgaa gaatgtctgg tgatagaaga ttcttataat
     4021 ggtataaaag ctggtaaaag agcaaatatg aaggttcttg caattaaaga taaacgtttt
     4081 tctcaagatg taagtctagc agatgatgtt atttacgata ttaaatatct gcgacaagaa
     4141 gtacaggtga aatttggaat ataaataagt tttttgatgt atttagagaa tattagtaaa
     4201 aataaaaatc atgcccaaat ctaatcaaaa ttgattgctt tgggtatgat ttcttttaat
     4261 atcaatctat tactttagga tctgaatcat ttttatgcac cgtatctaaa gtgatatttt
     4321 ctgtgacttc aataacataa ctaattctat cacaacgaac tgtggattga gaataatgaa
     4381 atggttgatt atttttatct ttgccaacct tttctaaaat aaatagcgga tctcctacta
     4441 tacattgcaa taagtcggaa agctcaggtg ttgcagataa accgtttatt tccataatag
     4501 agctcgtagc tttgacatca tagtcatctt ctagaagttt atataatgaa gtatattggc
     4561 tacctttttc aaataaagtt ggaaattttt cttctgatgc gaaaaaatta tcaattccca
     4621 tcggggcacc gtcttcccac attaaacgtt taatattgta aactctatca gttacattga
     4681 tttgtaattt attagctaaa aactcatcag cctttataat tttagaatgt aaaacagtat
     4741 atctaatttt atgtcctcgg gtagaaagag aatcgctaaa tccatgaact ccagctaatt
     4801 ttaaactttc ttttttttga gttacaaagg tccctttccc ccttaatcgt tcaactgttc
     4861 cactttctgc taaaagttta aaagcttttc gaatggtaat cttacttact ccataaattt
     4921 tacataattc ttcttctgta ggtaattgct ctcctggaag atattttttt tcacgaatat
     4981 cgttacgtat agaagcttct acttgtaaat ataatggtct attatcttga aaattcatag
     5041 tacactccct aaaaagttat acttcattat atcacaattt attaaaacgt cgattgtaaa
     5101 attaagctag acaactaaaa agatcatttg tgctacactc aaaatagttc cgaccaaaga
     5161 attaataagg agtgagtaca aatgacttac acccatctta caacagacga actagtaatc
     5221 atagagtctt atttcaatat cgagaaacct gtcgctcttg tcgcacaaag tttaaatcgc
     5281 tctagacaaa ccatttataa tgtttattcg tttctcaaac aaggaaaaaa agtgctagat
     5341 tattaccgtc aatacaaaca aaataaatct cgttgtggac gacgttcaac cgttttatct
     5401 gatgaacaat caacatatgt tcaagaaaag gtagctcaag gatggtcacc cgatgtgatt
     5461 atcggccgtg gcgagtttcc aattgattgt tctgttcgta cactctatcg gatgtttaaa
     5521 aaaggccttt ttgacacaca gtcgttacct atgaagggga aacgtaaacc gaataatcat
     5581 caagaaaagc gtggaaaaca ggcctttcgc agaacgattc atgaacggaa cagtctctat
     5641 ccacacctga ctaaagaatt tggtcatctc gaagtcgaca ccattgtggg agttaaacac
     5701 aagagcgctg ttattacatt ggttgagtgt ttatccaagg ttattattac tttgaaaccg
     5761 acaggcagac gagccgaaga tatcgaaaac aggttaaatg attggttcaa atccatg
//
LOCUS       DACDSY010000153         5809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDSY010000153 DACDSY010000000
VERSION     DACDSY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.156"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            450..1604
                     /locus_tag="IWK44_002509"
     CDS             450..1604
                     /locus_tag="IWK44_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP9550979.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="IWK44_002510"
     CDS             1598..2020
                     /locus_tag="IWK44_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550980.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="IWK44_002511"
     CDS             3143..4075
                     /locus_tag="IWK44_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9550981.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="IWK44_002512"
     CDS             4092..4607
                     /locus_tag="IWK44_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9550982.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="IWK44_002513"
     CDS             4903..5184
                     /locus_tag="IWK44_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550983.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="IWK44_002514"
     CDS             5186..5602
                     /locus_tag="IWK44_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550984.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DACDSY010000154         5780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDSY010000154 DACDSY010000000
VERSION     DACDSY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.157"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(89..1219)
                     /locus_tag="IWK44_002515"
     CDS             complement(89..1219)
                     /locus_tag="IWK44_002515"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP9550985.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1867..3069)
                     /locus_tag="IWK44_002516"
     CDS             complement(1867..3069)
                     /locus_tag="IWK44_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9550986.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3129..3332)
                     /locus_tag="IWK44_002517"
     CDS             complement(3129..3332)
                     /locus_tag="IWK44_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9550987.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3474..4706)
                     /locus_tag="IWK44_002518"
                     /pseudo
     CDS             complement(3474..4706)
                     /locus_tag="IWK44_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4869..5681
                     /locus_tag="IWK44_002519"
     CDS             4869..5681
                     /locus_tag="IWK44_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9550988.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1709 a         1189 c          921 g         1961 t
ORIGIN      
        1 tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg actttttggg
       61 acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg acttgctgca
      121 ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct tctgttgaag
      181 caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt tgtgcttctt
      241 ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa gtaactaatc
      301 ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa tctcctgctc
      361 ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac tcttctgcaa
      421 tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg cagatatgtg
      481 ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc tctgtacaaa
      541 tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt ttagtttgat
      601 cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct ttttcataaa
      661 aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca gatagatcag
      721 gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt cgtaaaatca
      781 tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc actacttctt
      841 tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat ttatctacta
      901 cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat ccagtttctg
      961 gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa aatccacgcc
     1021 gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc actaattttt
     1081 ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct tcttgaactt
     1141 tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga tctactgtaa
     1201 atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa aaaaatcctt
     1261 ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt tttcagaaaa
     1321 aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa agactgttcg
     1381 tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc aacttctagc
     1441 tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct tctcatcgga
     1501 tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg gaataagaac
     1561 aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata aagcttttct
     1621 aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa tgatcatacc
     1681 cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa actaagtcct
     1741 ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca tcttgaacag
     1801 gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg tagaaccgtg
     1861 gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct cttgaacatt
     1921 atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt aagttcctaa
     1981 agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt gaatattctg
     2041 ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg gtttgacacc
     2101 cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg taaatgttcc
     2161 gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat accactgtct
     2221 gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt tagttttggg
     2281 agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa tcgttatcgt
     2341 gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa tccgaatgcc
     2401 agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct tatcatcctt
     2461 caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt tcttattctc
     2521 agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct ctttaacagc
     2581 ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt ttgataacgt
     2641 ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt caaataacca
     2701 atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa actgcatacg
     2761 cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc tctcgtacca
     2821 ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag aacacagttg
     2881 attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag caattttggc
     2941 atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat tttcatctat
     3001 acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa tatttttaat
     3061 ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata gtaccgcgct
     3121 tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg ggatggcacc
     3181 acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag atatgccttg
     3241 ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat caaaattgca
     3301 cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc tttactcctt
     3361 ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt atactcgtct
     3421 tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct tttttagtag
     3481 cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac ctcctcctga
     3541 taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg attcatttct
     3601 ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac ctgtgcaata
     3661 aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc aaaactttga
     3721 ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc tgcccatttg
     3781 gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat tgctccatta
     3841 agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt tgccaagctt
     3901 tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc ctcttctaaa
     3961 ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa acgacactga
     4021 ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat gatatccgta
     4081 ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat taccttatgc
     4141 ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc aatccgtgta
     4201 aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt ttttcgtgtt
     4261 tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac agttccttta
     4321 accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta ttggccaata
     4381 tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc aataatctct
     4441 tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc aatcaaaaca
     4501 cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa tttttcttca
     4561 cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat ccccactttt
     4621 tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat ggcttcaccg
     4681 agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta cttgtcaaag
     4741 aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat tttaagtcaa
     4801 tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta tcctgaggag
     4861 gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag attcgccaag
     4921 aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg cgaagtacag
     4981 tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac caaatcgcag
     5041 agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat caatacattt
     5101 tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt ctcatagtag
     5161 acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg attgaatttt
     5221 ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa tgtatcgatg
     5281 aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag ctctatttgc
     5341 aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc aactttagtg
     5401 tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt gggaaacatt
     5461 tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa ttgaccagcg
     5521 atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag tacacagagg
     5581 acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg gaagtcgaga
     5641 aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa attgataagc
     5701 acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac agtttcctgt
     5761 tctttgttag ccccctgtta
//
LOCUS       DACDSY010000155         5723 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDSY010000155 DACDSY010000000
VERSION     DACDSY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.158"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..89)
                     /locus_tag="IWK44_002520"
     CDS             complement(<1..89)
                     /locus_tag="IWK44_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9550989.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGL"
     gene            189..1910
                     /locus_tag="IWK44_002521"
     CDS             189..1910
                     /locus_tag="IWK44_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9550990.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            1925..3709
                     /locus_tag="IWK44_002522"
     CDS             1925..3709
                     /locus_tag="IWK44_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9550991.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(4090..5643)
                     /locus_tag="IWK44_002523"
     CDS             complement(4090..5643)
                     /locus_tag="IWK44_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9550992.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
BASE COUNT         1778 a         1103 c         1095 g         1747 t
ORIGIN      
        1 attaatcctt gttcatccca atagcggatc gttgattttg gcaaatcaaa tagttccgat
       61 acttggccaa tcgtatacat ttttttcatt ttttctcacc tctttttcag tttattcttt
      121 tttctattga ccttcaagtt acttgaaggt atagaatcga aaagtatcag gaaaaaagga
      181 ggaataatat gtctatctta aaaacattct tacagaaaaa caaaggactc gctctcctta
      241 cctttgtgat gatctgttta gaaatcgccg gtacattagg tgtaccaaaa ttagtagcaa
      301 agctgatcga cacaggtata gcaagtggcg aagcctcagt tataaaaaaa attggagttg
      361 aaatgctgct tgtagcgttt ctaggaacaa tcgctgcagt tgcctctagt tacttttcag
      421 cattggcttc cgctaggttt ggattccaaa cgcgggagaa atatttccgt aaattccaac
      481 agctgtcaat gaaggatatc agtcgtttta gttctggatc tcttttgaca agaatgacga
      541 atgatgtcga taatgtccag caaatgattg ttttattttg ccaaatgatc ttaccagcac
      601 cggttatttg cgttttcacc attctcatga tgtttcgtta ttctctatta ctaacatggg
      661 ttacattgat ctctgttgtt ttttatgtat ggattgttta tcgactgatg aaacgagggg
      721 cacctttatc tttgagtatc cagccaaaaa tggatcggat caccatcaca ttgagagaat
      781 tctttactgg gattaatatg atccgtgcct ttgataatca atcatatgaa gaaaagcgaa
      841 cgaataagag ctttgctgac tatgctaatc aaatgatcaa agtgaaccga atatttgctt
      901 gggtcactcc aattgctttt ttgctcatgg gaatcgttta tgcttctatt ttgtggtttg
      961 gcggaaatct tgtagcagaa ggagcattac aaatcggtgt cgtgactgct gtagtcgaat
     1021 actctatgct gacacttgct tacttgatga tcgcagctat ggtattggtc attatcccta
     1081 aatcgtttgc ctcattacga cggatagaag aaatactgca agcagagatc gaaattgagg
     1141 aagagacgat caaaacagta gctgaaccaa cctttgatca gacaaatgca gctgcttttg
     1201 aacatgtcac atttaaatat actgaaatct ctgatccagt tctagaagat attgatttca
     1261 caatacctaa aggaaaaaca acagcgatcg ttggcgggac cggatctgga aaaagtagtt
     1321 tggcgaaact tctcttgaga cttagtgacc ctacgatagg aaaagtaacc cttggtggta
     1381 ttcctcttac tcaaatgact caagaacaga tccgctccca catcagttat gtccctcaga
     1441 aggctttctt attcagcggg acaattttaa gcaacttgca gatgggaaat gaatatgcct
     1501 caacagaaga gttgtctaaa gcgatcaaga tcgctcaatt agaagaacta gtggacaccc
     1561 ttcctgacgg attggacagc tttgttgctc aaggcggttc taattattca gggggccaga
     1621 aacagcggat atgtattgct cgagcgttga tcaaaccagc agagatctat gtattcgatg
     1681 atagtttctc cgctttagac tataaaacag atgcagcatt aagagcagcc cttcataaac
     1741 acatgtcgga gaaaactctc gtgattgttg ctcaacgcct cagtacaatc caacaagcag
     1801 ataacatcat tgttttagat gaaggaaaaa tcgttggaca aggtactcat gaagaactac
     1861 tgtctaattg ccaaacttat caggagtttg caaaatcaca aggaatatag aaagaaggga
     1921 tacgatggaa aagaaaaaaa cgttgaacac gcgtgctttg atacgttttt ggaaaatgat
     1981 caaacctgaa cataaatatt tttacggatt gtttctatgc agtttattcg ggaacctatt
     2041 agtcgtagcc atgcccttga tcatgggaat cgggatcgat gatctcttat cagaaataag
     2101 aaaaacaggt attggacagc tttctttcag tgatatcaaa caagcattgt tgttccctgt
     2161 cttattgctg atcggctgtt cagtcttaag cagcatcact tcatttattc aggaacggac
     2221 aatggcagcg ttaagcgaaa agatcacatt gagggtgcga aaagaaatca caaaaaaatt
     2281 caaaacatta ccgatgtcgt tttttgacaa tcatcaagta ggtgacatca ttagtcggac
     2341 aactaccggt ctcaatcaac tttctcaagt gttattgaca ggtatcaatc aattttttac
     2401 ttctgtcgta acaatagctg ctgctggtct gatgctgttt tatatcgaaa gcaagcttac
     2461 tttgctcgtc ttattgctga tcgttggcag ttcaattgtt acaactaaga ttgcgaacaa
     2521 aaacaaacag atttctgaac agaatcagta tgaactgggt gttttaaata atcgaatgga
     2581 agaaatctta gctggtaatc tagtggtcaa aacattcaat cagcaagctc atgcagaagc
     2641 tgctatacac gaagtaaata aaaagcaata caacgctttt aaacgtgctc aatttatgaa
     2701 ctgtgctatc tatccggcca ttcgtttcat taatcaactc gccttcatca taagtgcggt
     2761 attcggtgct atgcttgttc tatcaggtgg tgtgacgatc ggcttcttac aagcttatct
     2821 tcagtacatc aatcagattt ctgaaccaat ttctaccgct tcttatgtga tcaactcgat
     2881 ccaatccgca atggcagcaa tcgataggat atttgagata ttagatgaaa cagatgaaat
     2941 tccagacctt ccagatgcaa gacttttaga acaaccgaaa ggaaaaatag ctttcaaaaa
     3001 tgtacagttt ggttacacat cagataaaat attgatgaat caagtcgatt tttcagtaaa
     3061 gccaaaacaa acggtagcga tcgttggtcc aactggtgca ggaaaaacga ctttagtcaa
     3121 tctgctgatg cgtttttatg aactgaataa aggaaagatc gaattcgacg gtcttgatat
     3181 taccaagctt tcacgtggag aactacgtcg ttcttttggg atggttttac aaaatacctg
     3241 gctgtttgaa ggaacggtag ctgataatat cgcctacggg aaaatggatg ccacaagaga
     3301 acaaattatc gaagcggctg aaatcgcaca atgtgatcac tttattcgaa cactgccaga
     3361 cggatatgat acgatcattt ctagcgaaaa cggcacgtta tctcaaggac agcaacaatt
     3421 attgacaatt gctcgagtca ttctagcgaa tcctcctgtc gttattttag atgaagcaac
     3481 ttctagcgta gatacacgaa cagaagcatt gattcagcaa gcaatggaag ctgtgacaaa
     3541 agataggaca agttttgtga ttgcacaccg tttgtcaacg attgaaaatg cagacatgat
     3601 ccttgtaatg aagaatggcg atatcgtcga aaaaggaaca cattctgaac tactccaatc
     3661 accaacgctt tatgccagtc tttataatag tcaatttcag atagcgtaac aaacattatc
     3721 taaaaagcta tcgatcatgt atgattagtc catgatcgat agctttttta aatgtactag
     3781 tggtgccact agaaacacaa agaataatac taatgatact ttttggatag ctccttcagt
     3841 ttcgttcggt aagtagtgac aaaagacttg tcacaatccc tttttacgaa taaacggtgt
     3901 tcaagagaca acctatcggc aataagacac gatcttgtaa aatacgagaa gctatacttc
     3961 aaaatcgctt cttgttgctc ttgtcaactc cgaatcacga gttacactat tcaacaaata
     4021 tgagaagctg ttttttgaat ttgtttcttg tgactcggtg ctgaacgtca ggctcacaat
     4081 ctctcttctt taatctcctt gttctacttg cgatagttgc tgaacgaatt gccatgtttc
     4141 cccgttatca gttgaacgga agaggcaggc aaacattttc cctgcatcga ttggtccttt
     4201 tttcaacaaa taaaattttg tctctaacgt attcgcgctt atttgagtaa cttcatttgg
     4261 tgattgaaaa agatctatcc cccctgtctg atagttttcc ggaatcgaca gaactgattc
     4321 tttgaatgag cgtcctccgt tgtttgtata aaacaaactt tcacgtgtcg aaacaaaacc
     4381 taaagaagtt gtaacgaagc tgacattttg tactggctga ttgatcattg tacttccact
     4441 ttttatccaa gtgattccgc catccatagt agtgtaaatg tacaaagttt ctgcagacat
     4501 cccactggct gatgtacaga taaatagacc atcgttgcca taaaataccg cttttcgata
     4561 acgtatcggc tgaactgatt gggtgataat agacgtttgc catgttttac cattatctct
     4621 tgaagtcagc agacgtacat catccccaga agaatagata aaccatgaga aatcggcgga
     4681 aatatcatat gtgttgtcca tccaataacc ttcaggaatc agccccgttg tcagggtgta
     4741 ttcgccaaaa cgcaaccatt gcgaatcaat cggcacaaat acccaattag caccactatc
     4801 tgttgtcata tagagagagt gcgtctctgt atcaagtttg agatcattta tcttttgtgt
     4861 tgtcaaattt accttttgct taggatcagc taacaaccct tcatttgtct tttgtatatg
     4921 cgtatcgtta gttgagttca ttgtcatgtg agaagcagca tttatatgag aagacatttt
     4981 tggctgagtg tatctcccat aatagattgt tccagctaaa agaatgagga aaaaaccagt
     5041 gactaatgta ctcatcttag ttgtcaggta tccaccaggc gaagactggc ttttttccca
     5101 tctgggttcg ttttctagta aaaaaatatg gaatgtgttc attccgaatt ttgatttgct
     5161 ttcgcctaag tagataggta aatcatcaag tgatctcagc aataactggc aatcactttt
     5221 ttgtatccga aaagcttgac tcgtcgaagt cttctcaaaa taaagatagt catcatcttg
     5281 agaagttggt ttattatgta aaaactgttg tgctctttga ctaagccatt gatcgaaatg
     5341 acgcgacaat tgatggtaac ggagccaaat aagtagaaga aaaacaataa gcacaacaat
     5401 tgttattccg ccaactcccc acgaaaagtg atacaaatta tttaattttt gcatcccttc
     5461 agctgaacgg actactgcac tcatatggcg atataggaac gatacagctc cgaaaaaaag
     5521 caaaccagag aaaaatacta agcttaaaag cttgaacatt attcttttcg ctcttttttt
     5581 cgctttctcg ataatttttt gtttagctga taatacttcg ataataaata gttcttgatc
     5641 catttgtctc tccttttctc atacttcatt cattatgcgt caaaatccat acaatcacta
     5701 gtcccactaa taaaaaattt atg
//
LOCUS       DACDSY010000156         5582 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDSY010000156 DACDSY010000000
VERSION     DACDSY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.159"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(59..2008)
                     /locus_tag="IWK44_002524"
     CDS             complement(59..2008)
                     /locus_tag="IWK44_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9550993.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="IWK44_002525"
     CDS             complement(2050..3108)
                     /locus_tag="IWK44_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP9550994.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="IWK44_002526"
     CDS             complement(3227..3682)
                     /locus_tag="IWK44_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9550995.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="IWK44_002527"
     CDS             complement(4266..5069)
                     /locus_tag="IWK44_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9550996.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1647 a         1101 c          773 g         2061 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     5461 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     5521 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     5581 ga
//
LOCUS       DACDSY010000157         5577 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDSY010000157 DACDSY010000000
VERSION     DACDSY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.160"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            88..1080
                     /locus_tag="IWK44_002528"
     CDS             88..1080
                     /locus_tag="IWK44_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9550997.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1140..2561
                     /locus_tag="IWK44_002529"
     CDS             1140..2561
                     /locus_tag="IWK44_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP9550998.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2554..4053
                     /locus_tag="IWK44_002530"
     CDS             2554..4053
                     /locus_tag="IWK44_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP9550999.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4092..5486
                     /gene="uxaC"
                     /locus_tag="IWK44_002531"
     CDS             4092..5486
                     /gene="uxaC"
                     /locus_tag="IWK44_002531"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9551000.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         1825 a         1035 c         1154 g         1563 t
ORIGIN      
        1 tcatatcaaa gatctattga cttatcgtag ttatcgtaat atgatgaatg cgttaacaaa
       61 aagtgaaaaa gtaaggaaaa tgatggaatg gtaacgatca aagatatcgc taaagcagct
      121 ggcgtttctc atacgacagt ttctcgtgct ttgaataaca gcagtttaat aaaaaatgaa
      181 acaaaacaaa aaatacgaca actagctaag gaaatgaatt attcgccaaa ctatagtgca
      241 aaaggtttag tgaatcaaaa aaaatatcta atcggactat ttttctcaag tttgcataaa
      301 ggaacttcat caaatttcct agcagattcc attgcaggca tccattcagt attagacaca
      361 aattacagtt tatctgttga aagtatcgat gttgttgatt taacagaaat caacctgcaa
      421 cgctacgatg gactgatcgt tatgagtcaa agtgatgatg atcagccttt catcgactat
      481 ctcaaagaaa aagaatttcc tttcgttgtt gtcaatcgtc acatcaatga tccagcggtt
      541 atcaacgtca ctgccgatga tgcggccggt gttatgcagg cagtagatta cggtattgga
      601 tgcggacata cgaagatcgc ttatattggc ggaaagaaaa attttcgttc gacaaaagaa
      661 cgggaaaaag gggtattgaa aagtctaaaa aaagcgagca tccctgtcaa tctagcctat
      721 tttttaacag gagattacag cttagaaagc ggcctccaaa acatggaata cctattatca
      781 ctttctgacc tgcctactct ggttttttgc ggcaacgacg acatagcgat tggtgcattg
      841 agagctgtac atttagctgg cctccgtgtt ccccagaaca tttctctgat cgggttcgat
      901 gattctccag tcgtctccta tctcaatccg ccgcttacca ccgtacgtaa gcctttgaaa
      961 gaaatgtgtc aagaaggaac aaagctttta ctagatttga tcgaaggcag gacgatcgaa
     1021 caatctaaaa ttgagctctc caccttgttg atccttcgtg agtcgatcaa ttacttgtaa
     1081 actacaagta attttatata aaaatgttaa cgtgtgcata acacagaaag gaagtttata
     1141 tgaaaaaact gacaaaagaa ttagtgaaaa aaaacgaagc acctgtaaaa gttcttcagt
     1201 ttggtgaagg caattttatg cgtggattta ttgattggca gattcagcag ctgaataacc
     1261 aacaattgtt taaaggtaat gttgcaatca ttcaaccttt agaacaaggt cttggaaata
     1321 tgatgaacca gcaagataat ttatatacgg tcattttaca aggactcagc aatggcgaaa
     1381 tcgttgatac ttccgagatt atcaccgtag tagaacaaat gatcaatcct tatgatgaat
     1441 gggatgctta tctatctcta gcagaaaatg acgacttggc ctatatcgtc tccaatacaa
     1501 cagaggcagg aatccaattc gtggagactg ataatttaac agatactccc ccacagagct
     1561 tcccgggcaa actgacagcc tttttatacc gacgtttcaa attaaacaaa gctggcttta
     1621 ccatcattcc ttgcgaactg attgacagaa acggcgaacg tctaaaagaa atcattttac
     1681 gctacgcaga aaaatgggat ttagaacctg cttttgttga gtggttagaa aaagaaaatg
     1741 ttttctgttg tagtttagtt gatcgcatcg tcccaggtta tcctcgcgaa gatgcaggtg
     1801 ttttcaatca acggcataac tatgaagatc aattgatggt caaagcagaa ccattcatgc
     1861 tttgggtgat cgaagggcct aaagaattgg agaatcaact accattcaaa caggctggct
     1921 tgaatgtcat catcacagat gatatgactc cttatcgtga acgtaaggtg catttgctta
     1981 atggaccgca tacagcgatg gtccctcttg ctttgttggc ggaattggaa actgtagaag
     2041 aagtaatgaa agatccttta ttctccgcat atgttgatca gttattcaat ctagaattga
     2101 ttcctatgct ttctttacct aaagatgaat tagccattta tgctgaccaa atcaaagaac
     2161 gtttcttgaa tccttttgcc catcacaaat tagaagcgat ttcattaaat agtgtttcaa
     2221 agttcagcac ccgtctgctt ccagtgttca agaagtatat cgaagaacaa aatcaggtac
     2281 caccgttaat cactgtttca ctagctgcgc tacttcttat gtatcgaggc gatcaagtaa
     2341 agccacatga tgatgaaaag acgataagcg aatttactga tgcttggtct gataatggaa
     2401 cagctattcc tcgattattg caaaacgctg cgctttgggg agaagatttg tctcaaatac
     2461 cgaatgtgac agatacagtt caagaaatag ctgcaaagat cgatcaaaaa ggtgtccggt
     2521 cagtattaca agaaatgaaa ggaacgatgg tccatgcttg aaaccctgca accaacaatg
     2581 aaattaaatc gacgagacag tgtggtcgtc gcattaaccc ctattccaaa acaaacacaa
     2641 ctgacgattg atcagcaaac gatcaccact cttgaagata tcccgcaagg tcataagatt
     2701 gcattgggtg atttgaaagc tggagacaac gtgatcaaat atggctatcc aatcggtcat
     2761 gtgacgacag acgtaactgc cggtcaatgg ctgcacactc ataatgtaaa aaccaatctt
     2821 tctggtgaat tggattatcg atatgaaaaa gatgtccatc caagtcatta cctttttgag
     2881 aaccgaacat ttcaaggtta cttgcgaaaa aatggaaaag tagggattcg taatgatttg
     2941 tttttagtac ctaccgttgg gtgtgtgaat ggcattgctg aactgatcgt caaacagttc
     3001 aaggaaaaac atccagatct gggacagttt gaccatatta cgattttaaa gcatccttat
     3061 ggctgttcgc agcttggaaa agatcatcaa aatactcgag aaattttagc ggacgcagta
     3121 aatcatccca atgccggagg agttttagta tttggattag gttgtgagaa caatacagtt
     3181 cctgaattca aaaaaatttt aggtgaatat gatgaagaac gagtgaaatt tctagtggct
     3241 caagatgttt acgatgagat cgaacaaggt gtggctctat tagaagagct tttaacagcg
     3301 gcggaaaatg accagcgcat atcagtcccg ctctctaagt tgaatgtcgg tttgaaatgc
     3361 ggcggttctg atggtctatc gggaataact gctaatcctt tacttggtgc attttctgac
     3421 tacttgattg ctcaaggagg cagcacgatc ttaacagaag tgccagaaat gtttggcgca
     3481 gaacaagtat tgatggcacg agcggaaaat gaagaagttt ttgactcaat cgttgacttg
     3541 atcaatgact tcaaacagta tttcttgtct tacggggaac cagtttacga aaacccatct
     3601 cctggtaata aagcaggtgg gatcactaca ttagaagata agtctttagg ctgcacgcaa
     3661 aaatcaggaa gctctcctgt cgttgatgtg ctacaatatg gcgaaaaaat cagaaaacca
     3721 gggcttagtt tattgcaagc tcccgggaat gatcttgtag ctgcctctgc tcttgcttca
     3781 tccgattgcc agttggtctt gtttaccaca ggaagaggga ctccgtttgg ctcatatgtc
     3841 ccaacggtca aggtatctac gaatacaact ctttttgaac gcaaaggtca ttggatggat
     3901 tttaatgcag gcgtcttatt agaacaacca atggaagtaa tcttagaatc gtttgttcaa
     3961 aaaatcatcg ctgtagctag tggagaagaa acgaaaaatg aacaaaatga cgttcgagaa
     4021 attgccattt ttaaaaacgg agtaacactt tgattttcat aaaacagtat aaaaagaaag
     4081 aaggaagatt tatgttttta tctgatgatt ttttattaaa agacgattgg gcgaaaaagc
     4141 tttaccattc ctacgcaaaa aacatgccca ttattgatta tcactgtcac ttagatccaa
     4201 aagaaattta cgaaaacaac aatttcagca atctgacaga agcttggtta ggaggcgatc
     4261 attacaaatg gcgcttgatg cgagcttgcg gcgtgcctga agaaaaaatc acaggaaatg
     4321 cttctgattt tgaaaaattt ttagcttggt gtcaaaccgt accaaaaatt atcggaaatc
     4381 cgctttacag ttggtcacat cttgaattga agcgtttctt cggtattgac ttgttgataa
     4441 atgaagaaaa tgcagagaaa atttggacac aagcaaatga aattttggca gctcctgatt
     4501 ttcgccgtcg ggaaatcgca aaaaaatcga atgtagaagt gatttgtaca acagatgatc
     4561 ccgcagacga cttgcactat catcagttgc ttgctcatga agaaccagat ttgaaggttc
     4621 taccttcatt ccgtccagat aaagcactca atattgaaaa agaaggattt aatgcatggg
     4681 tcacactact ggaagaagca gctgaccaaa cgatcaccac ctatcaagaa ctgatagaag
     4741 tcttagggca gcggatagaa tacttccatc aacatggatg tcgtgtttcc gaccacgctt
     4801 tagacatctt acgctataaa gaagcggatg aaacagtatt agaagagatt tttcaaaaag
     4861 cacgagctaa caaactatta tcttcagatg aaatcgatgc ttatcttaca gaaacactga
     4921 tacatttgac ccacttctat cattctcata attggacgat gcagctgcac atccatgctt
     4981 atcgtaactg taatacacaa atgttggaaa aattagggcc cgataccggc tatgatggaa
     5041 tgaatgatct ttctttgact attcctctgc aaaaattatt gaaccgtgca gaagaaacgg
     5101 atcagttgcc aaagacgatc ctttattcat tgaatccgaa tgactatcct gcgattttag
     5161 ctttgatggg ctgtttccaa aaagaacaga atggaaaact gcagttaggt tctggatggt
     5221 ggtacaacga tactcgagct ggtatgcgcg atcaattgac acaatttgct gatggtagtg
     5281 ctttggggaa ttttgtcggc atgctgacag attcaagaag tttcctgtcc tacactagac
     5341 atgaatattt ccgtcgtgtt ctttgtgaat ttttaggcga aatggtcatg cgaggagaag
     5401 cacctgaaga tgaacaactt ttaggaagtc tagttcaagc gattagttat acaaatgcta
     5461 aaaattactt tggctttttt gcctaattga ttaatagtaa cagtaacgaa tcaaaaacag
     5521 gatagtgaaa taacggaatc cgtttatcca cgttttagag aagaagacgg accaaat
//
LOCUS       DACDSY010000158         5468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDSY010000158 DACDSY010000000
VERSION     DACDSY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.161"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            117..1748
                     /locus_tag="IWK44_002532"
     CDS             117..1748
                     /locus_tag="IWK44_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9551001.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1751..2830
                     /gene="uxuA"
                     /locus_tag="IWK44_002533"
     CDS             1751..2830
                     /gene="uxuA"
                     /locus_tag="IWK44_002533"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9551002.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2904..4331
                     /gene="uxaC"
                     /locus_tag="IWK44_002534"
     CDS             2904..4331
                     /gene="uxaC"
                     /locus_tag="IWK44_002534"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9551003.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            4415..5062
                     /locus_tag="IWK44_002535"
     CDS             4415..5062
                     /locus_tag="IWK44_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9551004.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            5191..>5468
                     /locus_tag="IWK44_002536"
     CDS             5191..>5468
                     /locus_tag="IWK44_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9551005.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALA"
BASE COUNT         1904 a          744 c         1151 g         1669 t
ORIGIN      
        1 aattctgaca tggtagaaaa aagaatattt ttagtttttt cttattgaca gcgattacat
       61 tcgattttat actacaattg taacggaaac taacatatca gtatggaggg gtaaatatgg
      121 ttttgattaa agactggcaa gattctatcg aaggattgaa caaaatgcaa gttcaactac
      181 cgtattttaa tgtcgaagcg gtcaaacaag aaggtcagaa acatccagtg tggcttcatt
      241 ttggtggagg aaatctttat cgaggatttc atgcagaaat tgctcaatgc ttagcagatc
      301 aaaatgaatt agaatccgga gtagttgtgt gtgagacatt tgatgaagaa gtagtagaaa
      361 aagcttatca tgactacaac aatgatattt tggagattgt tatgggtgaa gatggtgaac
      421 tcaaaaaaag acttttagca gtaacagctg atagtttata ttgcaatcca aagaatataa
      481 cggactatca aagagtggtt aaatattttg aagatcctac attacagttt acaacgttta
      541 ctattactga aaaaggatac agtttaaaag attctaaagg acaatttttt tcggtagtgg
      601 aagcagatat aaaatctgga cctaccaatc ctcaacatac gatgagtatt gtgacatcct
      661 tgttgtatag caggtttaat gctggagagt taccgattgc gatggtttct actgataatt
      721 tctcacagaa tggtcaacgt tttcaagata gcatttttat gattgcagaa gcttggcaaa
      781 aaaaaggata cgtttctgaa aaatttattg cttatttaaa ggataaagaa aaagtgactt
      841 ttccttggag tatgattgat agaattacgc caaatccttc ggaaagtgtc aaaatggatt
      901 tattgaaggc gggtttcgaa gacttagata taattcatac tgcgaaaaat accaatatcg
      961 ctgcttttac caatactgaa aaagtccatt atctagtcat tgaagatagt tttccaaatg
     1021 gtcgacctaa actggaagcg gctggtgtga ttatgacaga tagagaaaca gttgataaag
     1081 cagatatcat gaaggttacg acatgtttaa atccgctcca tacggcatta gcaataacag
     1141 gttgtttgtt ggggtataaa tcaattcagg cagaaatgca agatgatgat ttggttggct
     1201 taattaaagg gataggttat gtagaaggct taccagtagt tgaaagtcca ggcgttattg
     1261 atccaaaaca atttattgat gaagtaatca ataaacgttt gcctaatccc tatattccag
     1321 atacaccgca acgaattgca acagatactt ctcaaaaagt agcgattcgc tatggtgaaa
     1381 caattaaaaa gtatgtcgaa aaagacggaa atgcagaaaa tctaacattt attcctatcg
     1441 ctattgctgc ttggttacgt tatttattgg cagtcgatga tcaaggaaaa aattttgtgc
     1501 caagtcctga tcctttattg gtagagttgc aggaaaaatt ctcagtattg aaattaggtg
     1561 aacaagatcc tgcaaagatt cattgtgcgg tacaggaaat cttagaaaat aaggaaatat
     1621 ttggtttaga tttatatcaa gttgagatag gaaagaaaat tgaaaaaatc ttttgcgaaa
     1681 tgctagtagg tcctcaagca gttcgtaaaa caatcaataa atatataaca gaagttggag
     1741 gaaagtaact atggaaatga cttttagatg gtatggggca aatgaagaaa aaatctcact
     1801 acaagaaatt cgtcagatcc cagcaatgaa aggaattgtt ggaacattaa tggatatacc
     1861 agccggagag gtctggccga gagaacgaat taaagcttta aaagatgaag tcgaagccaa
     1921 tggccttacc ttgaaagtta ttgagagtgt taatgttcat gatgatatca aaattggttt
     1981 gccaacacga gatcaataca tcgagaatta taaacagacg attcgcaatt tggcagaatt
     2041 tgggattgag gttatctgtt ataattttat gccagtcttt gattgggtaa aatcagattt
     2101 ggattatcat ttagatgatg ggtcttctac gttagcctat atttcaaaaa atattcctac
     2161 tgatccagag gaaattatta gaaaagtaga aaattctact aataattttg aattgccggg
     2221 ttgggaacct catcggttat cacaaatcaa gcaattatta gaagcctata aagaagttga
     2281 tgaagcgaaa ttgcgagaga actttgcata ttttctcaaa gcaattatcc cgacatgtga
     2341 agaagttggg attaaaatgg cagttcatcc agatgatcca ccatattcta ttttcggatt
     2401 acctcgtatt gttaaaaatc gtgacgattt agaatggttg tgtaatgtag tggattctcc
     2461 ttctaatggt attacgttat gtactggaag tattgcagaa cgaccagata atgatgtcta
     2521 tgaaattcta tctgaatttt gtcaacggga tcggattcct tttgcacatg ttcgtaatat
     2581 caaatattta agtgataatg gactcgattt ttatgaagcg ccgcataaat cagaatatgg
     2641 ttctctagat atgtataaga tattaaaaac aatgtacgat aatggattta atggctatat
     2701 tcgtccagat catggtcgga tgatttgggg agaaaccggt agacctggtt atgggttata
     2761 tgaccgagct ctaggtgcag tttatttaaa tggtatttgg gaagcgcttg aaaagggggc
     2821 gagtaaataa ttatataatg gagtctattt agaagaaaaa ttttctcttc taaaaactaa
     2881 gggaaggaaa ggagtgctta aatatgtttt tagataagaa ctttttatta gagaatgaaa
     2941 tagctaaaag actttttcat aaatttgcag aacaccaacc aatcatcgat tatcattgtc
     3001 atttagaccc agcatgtttg ctagaaaata agcaatacaa gaatttgact caggtatggt
     3061 taaatgataa aggagctgga gatcattata aatggcgatt gatgcgagct aatgggatag
     3121 aagaagagtt gatcagtggt gcaggtaatg attatgaaaa atttttagct tttgtaaaaa
     3181 cagtggagaa ggcgccaggt aatcctattt ttgaatggtc tcatttggaa ttacaacgtt
     3241 actttgatat tgatttgaca atttctgaga agaatgcacc agaaatttgg cgacgtgcca
     3301 acgaaaaaat ccaaacggat tcctatcgcc caaaagaatt gctgaagatg atggatgtga
     3361 aagccatttg tacaacggat gatcctacag atgacttgcg atatcatact gagctttcta
     3421 aacaggataa ttctttcaag gtattaccta ctttccgtcc agataaggtc tggagtatca
     3481 aatcatcagg attttcgaac tatattttaa aacttagtca agcagcgggt ataccaatca
     3541 aaaactttaa tgggttgaag caggcattga gccaacgagt ggattatttt catcaaattg
     3601 gctgtcgatt agcagatcac ggtttgaata cttattttta tgaaatagca gatgaaaaaa
     3661 tactcgacaa tctgttaata aaagctttag caggaaaaac ggatttccat ttatcagaaa
     3721 ttgcacaatt tgttacaggt attcaattac atttgatgtc tgagtataca agatatggtt
     3781 ggacgatgca aatgcattta aatgctttaa gaaataattc aagtatatta aaggaacaaa
     3841 ttggagtaga tgtcggtggt gattcgatgg gggatcaagc tagtttgaca caaaatgttg
     3901 tagcattgtt cgacactgct gaaaagagtg ggtgcttacc aaaaacaatt ttatattcat
     3961 taaatccaac cgattggctt ccactagcaa cagcaatgca aagcttccaa gggggtatga
     4021 ttcaaaaaat ccaattggga tgtgcttggt ggttcaatga tacagctgaa ggaatgcgtc
     4081 aacagttgac tacgatggcg caacaaagtt tgttgtcgaa ttttacaggt atgttgactg
     4141 attctcgaag ttttctaagc tatcctcgcc atgagtattt ccgtcgagta ttatgtcaat
     4201 tactaagcga gtgggttaaa gcaggtcgaa ttccagatga tgaaatgtat cttagtcaga
     4261 tagtggagaa tatttgttat cgtaatgcgt ataattattt taattttttt gaaatcgaaa
     4321 gtagaaaata atacaaaaaa taaaagctta aatttattta ttgtaagtta gtacaactag
     4381 ctagagtatg tatccataaa agaaggagat agatatgaaa aaagtagaaa ttttaagtaa
     4441 gttagaagcg gctggagtca ttgcggttgt acgtggaaaa aataaagatg aggccattaa
     4501 agcaataaat gcaattactg cgggtggaat taaagggatt gaactaactt ttacagttcc
     4561 gaatgctgaa gaaataatca aagaaattgc taaaaaatat gctgatcaaa aagatgtaat
     4621 aattggagcc ggtacagttt tagatgcgtt tactgctcgt attgcaatta tggctggagc
     4681 agaatatatc gtaagtccat cttttgatcg acaaacagca gagatttgta atttatatca
     4741 aattccttat ttaccaggtt gtatgacaat tactgagata aaggaggcac ttaccgctgg
     4801 agtagaaatc attaaactat ttccaggttc tgcttttggt ccatctgttg taaaaacgta
     4861 tcaagctcca ttaccatatg taaatataat gcctactggc ggagtaagtc tagataactt
     4921 agaagagtgg tttgaagcag gcgttgttgc agttggtgta ggtgggaatt tgttggctcc
     4981 tgttgaaaaa ggtgattttg aagaagtaac tcgaatagct agtaaatata tagataagtt
     5041 ttatgagata aagaattatt aggagaaaaa ataaataata ctataaaatg taaaaaataa
     5101 atactaactc tttatacttc agagtatagt acgtaagttg gcagttcaac agaatgaaaa
     5161 cgcttaacaa taggaaggag cgcaaattat atggaaagaa aatttggtct aagagacaaa
     5221 ataggatata tgtttggaga ttttgggaat gatttcactt tcatttttgc tagttctttt
     5281 ttaatgattt tttatacaaa agtattgggg ataagcggag ctaccgtagg aacattattt
     5341 ttagtagcaa gatttattga tgcagttaca gacataacaa tggggcgtat tgtggatgga
     5401 tcaaaaccat ctaaaactgg caaatttaaa ccgtggattt tacggatgag tggtccagtc
     5461 gctttagc
//
LOCUS       DACDSY010000159         5415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDSY010000159 DACDSY010000000
VERSION     DACDSY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.162"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            367..555
                     /locus_tag="IWK44_002537"
     CDS             367..555
                     /locus_tag="IWK44_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551006.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="IWK44_002538"
     CDS             545..805
                     /locus_tag="IWK44_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551007.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="IWK44_002539"
     CDS             846..1073
                     /locus_tag="IWK44_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551008.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="IWK44_002540"
     CDS             1138..1710
                     /locus_tag="IWK44_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551009.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="IWK44_002541"
     CDS             1711..2022
                     /locus_tag="IWK44_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9551010.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="IWK44_002542"
     CDS             2013..2273
                     /locus_tag="IWK44_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551011.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="IWK44_002543"
     CDS             2434..2793
                     /locus_tag="IWK44_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551012.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="IWK44_002544"
     CDS             2790..4433
                     /locus_tag="IWK44_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9551013.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..>5415
                     /locus_tag="IWK44_002545"
     CDS             4468..>5415
                     /locus_tag="IWK44_002545"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551014.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSL"
BASE COUNT         1931 a          744 c         1128 g         1612 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cacta
//
LOCUS       DACDSY010000160         5232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDSY010000160 DACDSY010000000
VERSION     DACDSY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.163"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            170..703
                     /locus_tag="IWK44_002546"
     CDS             170..703
                     /locus_tag="IWK44_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551015.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            715..918
                     /locus_tag="IWK44_002547"
     CDS             715..918
                     /locus_tag="IWK44_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551016.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
     gene            1240..1620
                     /locus_tag="IWK44_002548"
     CDS             1240..1620
                     /locus_tag="IWK44_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9551017.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            1602..2516
                     /locus_tag="IWK44_002549"
     CDS             1602..2516
                     /locus_tag="IWK44_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9551018.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            2761..3450
                     /locus_tag="IWK44_002550"
     CDS             2761..3450
                     /locus_tag="IWK44_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551019.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(3571..3657)
                     /locus_tag="IWK44_002551"
     CDS             complement(3571..3657)
                     /locus_tag="IWK44_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9551020.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            4333..>5232
                     /locus_tag="IWK44_002552"
     CDS             4333..>5232
                     /locus_tag="IWK44_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9551021.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALS"
BASE COUNT         1882 a          918 c          989 g         1443 t
ORIGIN      
        1 acaagaattt aatataacat attttcctaa tgcattctaa ctataccaca ttatcaaaaa
       61 tataaattat ctttactaaa taaaatattg acaagcctat accttttgtt ttatgatttt
      121 cacaaagaaa acgttttcta tatatttttc tgtatcagga ggaaattcta tgaaaaaatt
      181 tactttaggg gtagctttgt taactattgg ggcagttgtt ttaccgtcaa ctactatagt
      241 ttatgcttca gaaaattccc aagtggtatc attaaacgaa aacaatgcta ctgaaaatgt
      301 agttattgag tacactgaag atggtatggt cgtaactaaa tatgaggatg gttatagtgc
      361 ggaagatttt gcaaattctt cagatatcat gacaagagct gcagttgcaa gatggggcaa
      421 aaaggaatat acaaatattg ctgtaaccac tggagtagct gctaatgcta ttaatgctgc
      481 cttttgggca ggagggaaag ctagcacttc tatgtttggt atcccaggtt gggcaattgg
      541 tgctttgtta acaggtgcaa gttggactaa aaaaggtgct tctccaggga atgcagttgc
      601 aaaagaatgg gacaaaaatg gtaatggctg gattggattt tattaccaaa ggggatatga
      661 tgcagctgga agagttgtag ctactagata ttcaacgtta taagttaagg agtgatgttt
      721 ttgatatcta aatcattaaa atcattaaaa acatttacta aaaagaatcc gcaaatagct
      781 actattcgta tttttatatt tgctatagct gttgctataa ttgttaatat gctaagcgga
      841 gggcaattat acaaatctgg tttattttta gtgttaatct tagttgtctt agacataata
      901 aagtctaaaa aaaaataaat cgatacattt tagaagaata gggttgtttg aatattacaa
      961 agcaacaatt tcaccttcgg tgaaataagc gaaactttac aaaaagtggc tagcaaatgc
     1021 tagccttttt ttgtattgtg gagcgtggat tttgaggggg attcaagggg gagcttgcgc
     1081 tcctttgaca agcggttatt tgcaccaaat acggaggtat ttggctagca aatatagagc
     1141 ttgccaaacc cttttagtga gctttactca ttgtaccttt ttaggtacaa tggtattgcg
     1201 aacgatacgt ccgtaggacg tactgaaagg gtgggactta tgagcgaaca acatcaaaat
     1261 ttggctagcg atcattctaa aaaatatacc tatcgatccg agccaaaaca aatcagtttt
     1321 cgagtgagcg aatccgaatt tgcaaagctg aagcagtcag ctgaagcttt gcaaatgagt
     1381 gtgccggctt tcgtgaaagc caaggcacaa aacgcgcgtc tggtgacacc gaaagtggcg
     1441 ccagacattg cccaggcaat cgcgcgcgat ttagccaaag ctggagggaa tatcaatcaa
     1501 attgccaagt ggtgcaatac acaccaacac gacgttgctc ctgatgatgc tcaacgttta
     1561 tccgaaaacc taaaaatcat gcaaaaggag ctacaaaaga tatggcaaca gttaaagtaa
     1621 gtcgaaccac ttcatgtagc cgagcaatca attacgcaga accacgagca accgtcaaaa
     1681 caggaataaa ctgtgacatt gattatgcca aaagtgaaat gaaacaaata cgcacgcttt
     1741 atggaaaaga cgatcgtgta caagcacact tattgattca gtcctttcga ccaggagaaa
     1801 ttaccgctga aaaagccaat caactagggg cagagtatgc gaaaaaaata gcccctaacc
     1861 accaaatcgc gatttacaca catacagaca aagaccatat tcataatcac attgtcatta
     1921 actccgttaa tcttgaaact gggaaaaaat ttcaagccca tgggcaagca tttttaaaca
     1981 aatgttatga cctaaatgac gagatttgtc ttgaacatga cttaacaatc acggaacgag
     2041 gaaagaaaac cgaaaaacga accatgtctg aaattaaatt aaaagaaaaa aatgaaccgg
     2101 tttggaaaga cgaaattcgg tttgcgattg atcaaacaat gaaaaacaaa aaaactcgaa
     2161 cgtataccca attttgtgat tcacttaaaa cattcggcat tcattgcttg aatcgcggaa
     2221 aaaattttac ttacgaattg gtccacaaaa agaaaaaagt acgatccaac aaactaggaa
     2281 aggattacga aaaggagacg attttacatg agctggacag acgagaacga acctacaatc
     2341 aacaacgaac caacgagcta cgaagagctc gagctagcat tcgcgaaact gcagaagaag
     2401 tacgaagaga acaacgaact tttacgcctg aacaatcgga aattagaccg actcgaagaa
     2461 accatgaaca acacgttgaa cgaggaacag accaccaaca agaattatcg ctctaaaacg
     2521 ttaaaagaca tcgaaaacga aataaagaga caacgtgcct ttcttgcgcc ttatacccaa
     2581 gaaaggatac aaactacgat ggattcacaa gtcaaaaaac tccaccaagc gctattagac
     2641 cagcgaaacc aagaacaaaa aaaggcttta attcaacgat cgatggatat tcttgccccc
     2701 ttatgttgct tagtactaac attattagcc ttttggttgg tatggggaga ataaacagac
     2761 atggcattct atttacccta tctactcatt tctgccagta ttttaggaat tatttggctt
     2821 agctataagg cttatcaaac aagatattca ttaaaaggct ctaaaattcg atttaagcga
     2881 tttttattgg tcggggttat atttgcctta tcaatcgttt taagtggttt attcggcgtt
     2941 ttagagggta ataaacgtgt ttcaagaagc attttattag gggatatcac tcaaaagtat
     3001 gaagcagcat gtgataaaaa gaaaaaagag caagcattag cacaaaaaat ggaggaattt
     3061 accgcttgtt atgaggacat gaacaatatt tttgtggatc aagagaaacg tttaacagat
     3121 aaaaacatga aaaaactcac tcgtttttat caaaacttac cagaaaaaca gcaaaaagag
     3181 gtacaagaaa aatatgagca agtaaaaaaa gacgtgcaat attttaaaga tacgcagaca
     3241 gaagaagctt gttgtaactt atttagcgat acaatccctt tttcaaccag tgaacaagaa
     3301 cgaaaagaac ggcaacaatc ggtcacatac gaacgttatg aaacactgct tcagcaagca
     3361 acaaacatac aaaatccaac gaaaaaagaa acggcattaa attatctaga aagtgtcaag
     3421 gaatggttgg atcagcaaca acaaaattga atttgattag caatcatgtt aaactgaacc
     3481 tgcaaatgat caaacattag atacacgaaa gccccagaga ggtcggattc tctggggctt
     3541 tttttcaggt tgatgtcgcc tttattgatt ttacttcttg tttcggttat tcaaccaata
     3601 actgaataaa gagacacaaa cttctacaag aagggggcaa atgaaccgtt caaacattag
     3661 atacacctcc ttccagtcgc aagactgtcg gataggcgac aagttattat accttacaaa
     3721 ataaaatctg tgtggtgttt ttaagtatcc cttattttcg ggttgttagg gcttgccctg
     3781 accgcctgta aggcgcttta agaacaagat aaggtttttt acccaagact gtcttaaaac
     3841 gacttagacg agctatcaga ggcttccccc acctccgtta ctgacttgtt tttagcaaag
     3901 gagcctttag gcgactgcgc agtacaatgt gagagcatag cgatcacact tttttagcga
     3961 ccgtagggag caaggttttg aattggcagt tttgaaaaat gcgcgaacct gttggtagaa
     4021 aaaagaagtg gtttttaggc gggaaatttt gtcaccctct tttataatct ctttttaaac
     4081 ctttttttaa acctctttac cgtcggctgt gtccttactc cttcaatgga tacagcgttt
     4141 tataaactac attttgtctg cttttaaact acattttgtc tgcttttaaa ctacattttg
     4201 tctgctttta aactacattt tgtctgcttt taaactcttt ttttgcactc acgtatgtta
     4261 taatatgcat tatcacagac aaaacgttgt ttataacgtc tgtatccatt gaaggagtaa
     4321 ggaggaatat ctttggcaaa tgaaatcgtg aaataccatc atgaattaaa taccattccg
     4381 ttgcggaaat ttacaccagt tgaaatgaat ctatttttct ctattgtgtc tagaatgcgc
     4441 gaaaaaggcg ataaaacagt ccgtcttact tttgaccaac taaaagattt aagtaattat
     4501 aaagcaactg ctaataatcg tttcattaat gatttagaaa tgacttatga taaattaatg
     4561 gatttacgtt ttggtaaaag aagtgctaat gggctcgata tagatagatt cgttatgttt
     4621 agtgagttta aaatacgtgg aactgctaaa gatccatatg tagacataca agtttacgaa
     4681 aaagctttac ctttactaaa caatttagat gagtgggttc gctattcact ccaacaattt
     4741 aacgaattac aaagtagcta tgcaaagact atgtttcgat tactaaaaca atatcgaact
     4801 aaaggttttg cttatttttc taaagaggat tttcatgaac tattagatat tccaaaaagt
     4861 tactggaaca accaagcaaa cgttgataat cgtgttataa agcctatacg acaggaatta
     4921 accgcaatct tcaaaggatt atcaatcaaa aagaaatacg gaaaagggcg aggaaaacct
     4981 gtcgttggct atcaatttac gtttaaacca gaaatcaaaa aagcagatga ttttaataaa
     5041 catgcaccaa ccaaagaaga acaaaataaa gcattggctt tagctgataa acaaaaagaa
     5101 tacgaagaac ctgctacaac aattaacgaa aaaatcaaac agaagaaacg agaacttgaa
     5161 ctcctccaac aaatcaaaca actagaaaaa gagaaagaag ctcttgaaaa acaaacagag
     5221 tccgcacttt cg
//
LOCUS       DACDSY010000161         5140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDSY010000161 DACDSY010000000
VERSION     DACDSY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.164"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(406..780)
                     /locus_tag="IWK44_002553"
     CDS             complement(406..780)
                     /locus_tag="IWK44_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9551022.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="IWK44_002554"
     CDS             complement(803..2203)
                     /locus_tag="IWK44_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP9551023.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="IWK44_002555"
     CDS             complement(2207..2617)
                     /locus_tag="IWK44_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9551024.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="IWK44_002556"
     CDS             complement(2635..3126)
                     /locus_tag="IWK44_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9551025.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="IWK44_002557"
     CDS             complement(3136..3927)
                     /locus_tag="IWK44_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9551026.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="IWK44_002558"
     CDS             complement(3920..4693)
                     /locus_tag="IWK44_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9551027.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4849..>5140)
                     /locus_tag="IWK44_002559"
     CDS             complement(4849..>5140)
                     /locus_tag="IWK44_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9551028.1"
                     /translation="VFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDG
                     ITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         1640 a         1039 c          674 g         1787 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     4921 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc
     4981 tccctctttc aaatgttcat ttgttattcc atcaccaatc ataattcgtt caatcattcc
     5041 agaaagatgt agaaatagat taattctcag aaaacttggg attttgacat cgtaatattt
     5101 ttcatacaaa tatagtactt tttctatctc attgaatact
//
LOCUS       DACDSY010000162         5113 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDSY010000162 DACDSY010000000
VERSION     DACDSY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.165"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            170..637
                     /locus_tag="IWK44_002560"
     CDS             170..637
                     /locus_tag="IWK44_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551029.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            634..876
                     /locus_tag="IWK44_002561"
     CDS             634..876
                     /locus_tag="IWK44_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551030.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            894..1196
                     /locus_tag="IWK44_002562"
     CDS             894..1196
                     /locus_tag="IWK44_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551031.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            1267..3321
                     /locus_tag="IWK44_002563"
     CDS             1267..3321
                     /locus_tag="IWK44_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551032.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            3321..>5113
                     /locus_tag="IWK44_002564"
     CDS             3321..>5113
                     /locus_tag="IWK44_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP9551033.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHF"
BASE COUNT         1948 a          770 c         1024 g         1371 t
ORIGIN      
        1 ttttttcttt attatagctc ttttttgtaa tgaatcacaa tatcaagaga aaaattttct
       61 atagaaaggt ggatttttat gagtattcgt tctccgtgta tccaaatgag gtttgttgct
      121 actttcgtta tttgatatac taaaaataaa caacgaaaga aggaataaaa tgaagaaaaa
      181 gcaactatgg tttgtctcta tgtttgcaat attttttatt attttaagtg gatgttcaag
      241 taataagaaa tcggaagcta aagaagataa tagtttattg caaccaggaa ctgtttggaa
      301 agaagaagta ggtggtctta ttcataattt aaaaattata gatgatacta cctgggaata
      361 ttctgaaagt gtatggcatc cagatcctgt acaaataaca gttaagaggc aaaaagacta
      421 tgaaggttta gaaagataca aaatagtaga ttctgcaggc attagggaat ttataaacaa
      481 acctgattca ttatttattg ttgttcccta tgagaaggat ggcgtaaaaa aaataatttt
      541 tcttggatct agcaaggatg agaagcgaac gaaagagaaa ttaattcatg atggatcaca
      601 atcaaataaa tacaaattac aaaagactag cgagtgattg ctagtctttt tatttgtcct
      661 gaaggaggaa tcacagtggc aaaagctaaa acgccatttg aaaaagccgc gtctaaaatc
      721 ttagaacgcc gcggtgtatc tgaaacacag tggaaacaag aagtccttga aaaagcacaa
      781 tttgacctat tacggggaaa agacaaggac ttagaaggat atatctatca acgtgaacgt
      841 gaaaagcttg ttttcgatga aattattaaa cactaaaagg agcgaacaat tttatgtttg
      901 aatcaattac aatgaatttt ttaacacaac cgaaagtagt agctgcggtt gatattttta
      961 gagcattaca aactgccgca actagtttga aaaataatct gatgattttt gctgctgttg
     1021 cggcagtggc ttccattgct cttggaggta ttttcttctt ctttgggcga agaggagctg
     1081 aaaacgggaa gacacacgta ggttatgtat tgttgggggt aggagttatt ttagctgccg
     1141 cttctattgt agtttcaatg ttctcaatgt ttggacaaac cccacaagga ctataaaaaa
     1201 acaagtagaa ggagtaaaaa gtacgtggct actttttact ccttttttta tggaaggaga
     1261 atattcatgt tttttacgat ttcaggcaat gcaaaattgg ataaagagtt aataagaaaa
     1321 gcaacgaaga aagtgggaaa gcgtacttcc tttttaccga aaacaattaa atattctgtc
     1381 attgaaacgg aagaacagct acatgtgtta ggacaagtgt tagaacaaga gaatcaatct
     1441 tctgttaaat tgactctcac gttacatgat aaagaaggaa aaatagaaaa ggctggcaca
     1501 gaatacgttt ttggctcact ggattatcaa atttcagaag gtttttatcc tacattgatt
     1561 gatttaatcc gcctagatat atttaaagat acggatgaaa ccatgagtgt ggaagagtct
     1621 tatcgaaacg aaaaaatttt acaagtttta gaagaagagt tagaaagcga ccaaagaaaa
     1681 gtgaatcaag tgagtgaagt cgaagcaaac gaagaagtac catgggaacg cccaatcttt
     1741 ccaaaggatg aatcaaaaga aaaagagaaa ggaattattg acgaacaagc tgacattttg
     1801 actcataaat caatatttga ggaagagaac gaggtaatgg gtcaattgga ggtagatccc
     1861 tttgaagaaa caatggagga tgaagagacg gccgaagtag aagttgatcc agttgaagct
     1921 aatgaagacc caacatggac tgaaggggat tctcaggaat atattgaacc acttatcagt
     1981 gaagaagtag agaaaaatga aacagagata caaaaggcaa cggtatttga ttatattttg
     2041 gatcaatatc cgccagagca tgaatggatg aaagaaaaaa ataaattatt tttaaatgaa
     2101 ctttattcga aatatcaatt acctcaaacc tatcaagcat ttttagagag taaagaagaa
     2161 ttaatctcac agggtaaagg cacactttta gaacagttag agtctgtcca atcaacaaat
     2221 tggcatgagc ttgcacaaga agatttatta gctacttttg aagaacgtca acaagaaaca
     2281 gatcaagaaa ttaatgagta tgcgacggaa cagtcgtcga attgggagaa agcaaaagag
     2341 gaactaaata aagaagaaca gcgagtgatt gaagaagaag tacaaaaaat taaagagcgg
     2401 tacgaaaaga aacgtgacaa tgcgtattcc ttgtgtgtag aaaagatcaa caattttact
     2461 cagacgaatc aagaagcatt atcgaaagaa aaagaaaact tattaaaaga acgtataaaa
     2521 gaacgaaaag aagaatttta ccgagggttg cgtactgata agctaaggat tagcaaagaa
     2581 ttaaatgaac aactgaataa actctatcaa actacgactg aagttctgat ggaaaaacac
     2641 gagcagatcc aacaagaatt acaagcacaa attccaaaat ggaaaaaaga gtatgaaatt
     2701 caccaacaag aattgaaaga acaagaagaa aaacaacgga aaaaagaaaa agagcgcgaa
     2761 caattagaac taaaaaaacg tgaacaggat cttgcggaac aaaaaatggc gatagaaaaa
     2821 caacggctag aacaagaaaa ggaaaaagaa gcagctgctc gtaaggaacg tcaagaacaa
     2881 atggatacat tgcgacaagc acagttaaat tatctgacac atcctcaacc agtagctgct
     2941 gttcaaacgc cagtgaacaa ccaagcaaat ttaactcctt caccccagcg ttcctttaac
     3001 ggatggatgg tgggatgtat tgcaagttta agcttgcttc taggtggagg aacagtattt
     3061 gcttttaatc atatcaatca atcttcacag gctgattctg tagccacaac ggcgtcaatc
     3121 aaggaagaag cccaggaaca agcaagaaaa gagtatgaac aacaattaga agaattattg
     3181 aatcgtacca atcagtcaac aacagagtca agcatggaaa atagcaaaga gagtgattct
     3241 agtaaggcta aagaaggatc aagcgattca actgtacaat caaacacttc cgaaagttca
     3301 gaatcaaatg aggtgaaata atgcgtgtta gagaaacaac gaagcgaaaa gacaaacgat
     3361 tcaatttaga aaagaatagg tatctttctc atttctatcg ctatactccc tcaggagaag
     3421 agctaccaga aaaacatgac aaacgcattt cgaaaaagat tttttatagt ggacttgtat
     3481 acctcccttt agagtattta gtaacaagag taccagaagt tttcaaccga ttggaaattg
     3541 ttaagggaag cttacaagga ataaatctgt ttcaattaca ggaaatgcca caggtctttc
     3601 attttgattc agctggtttt tggaatccat ttactcctac aactagtttg ggtacacgtt
     3661 tagcaatttc gacagtttgt gtggttccag ctgtacttat gggattgaaa atggacatgt
     3721 ggtttcgtcc tctagcagat ggacaaaaag gggataatcg attaatgacc ttaaaagaaa
     3781 tccaacagat ttatccgcaa atacctgaaa caaaaactac ttttccaggt tatggaggta
     3841 ttccaatcac acattacaaa aaatattggt acattcaaac agatactgtt aatacgtgta
     3901 ttgttggtac ctctcgatca gggaaaggtc agattgaagt gttagccacc attgacaatt
     3961 tatctcgtgc agaaaaacaa agttcaatgg tcgttaatga tccaaaaaca gaattatatg
     4021 ttggtagtaa agatgaacta gaagctagag ggtatgatgt atatgccttt aatatcaccg
     4081 atccactgca atcaatgagt gataatccat tggcactcat cgtgaaatac tggaaacgag
     4141 gagacataga tactgcaaca cagttaacca atactttcac aaatacgatt tattatgatg
     4201 ccaacgcttc agataacaaa tactttaacg aaaacgcgca gaaagctgtc aatgcgctta
     4261 tttttgcgtt gttagaacaa gctgatcaaa caggagatta ctccaaattg acaccgaata
     4321 atctcgttga gcttctttct gagattggcg gttttaatta ttatccagat ccagacaatg
     4381 aattcaaaca gttgaatgcc ttagatgaat ttatgaatca gttgccacct ggaaacattg
     4441 caaaaaaaca atatggggca acgaaaattg gatctgataa agcgaaggga aatattttat
     4501 caacggcaat cacgggattg aatccattta ctctaacgaa aattgcaaag atgacttctc
     4561 aatcaacgat tgattataaa tcggttgggt ttccaaaata cttagatttg aaattacatg
     4621 aaagtctttt aaatcaacga gtcgtagtag aattttatca taagggaaag aaatttcatg
     4681 aagaacgtgt caaagtaggc tataaaggat ttagtgaaat caattttgat tgtcatttag
     4741 ttgcaggtga tcaagtttgt ttaaaatacg atcatcaagg aaaagaatat gtggcttggt
     4801 atgaattttc tcaaagagta ttgaaagacg aagagggtaa tatcgtttat cggaaaaaac
     4861 gaggagaaac ccatttgcca gaattagaaa aagaagccgt tctaacatta gatgaggaaa
     4921 aaagtaactt attagtagag tctattcgga tgcactatac cgataaacca atggctattt
     4981 ttatgcttac acctgattat gatccatcta accatatcat tgttagtatt tttcttagtc
     5041 agttatataa agaattatct acgcaatgtg ttaagactaa aggagataaa tgtcaccgca
     5101 ggattcattt tat
//
LOCUS       DACDSY010000163         5017 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDSY010000163 DACDSY010000000
VERSION     DACDSY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.166"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..127)
                     /locus_tag="IWK44_002565"
     CDS             complement(<1..127)
                     /locus_tag="IWK44_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP9551034.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVY"
     gene            285..548
                     /locus_tag="IWK44_002566"
     CDS             285..548
                     /locus_tag="IWK44_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551035.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            572..760
                     /locus_tag="IWK44_002567"
     CDS             572..760
                     /locus_tag="IWK44_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551036.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(843..1028)
                     /locus_tag="IWK44_002568"
     CDS             complement(843..1028)
                     /locus_tag="IWK44_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551037.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            1243..1614
                     /locus_tag="IWK44_002569"
     CDS             1243..1614
                     /locus_tag="IWK44_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9551038.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(1764..1836)
                     /locus_tag="IWK44_002570"
     tRNA            complement(1764..1836)
                     /locus_tag="IWK44_002570"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1801..1803),aa:Gln,seq:ctg)
     gene            complement(1882..2310)
                     /locus_tag="IWK44_002571"
     CDS             complement(1882..2310)
                     /locus_tag="IWK44_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551039.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(2370..3128)
                     /locus_tag="IWK44_002572"
     CDS             complement(2370..3128)
                     /locus_tag="IWK44_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP9551040.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            3423..3620
                     /locus_tag="IWK44_002573"
     CDS             3423..3620
                     /locus_tag="IWK44_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551041.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3726..4208
                     /locus_tag="IWK44_002574"
     CDS             3726..4208
                     /locus_tag="IWK44_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551042.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(4266..4862)
                     /locus_tag="IWK44_002575"
     CDS             complement(4266..4862)
                     /locus_tag="IWK44_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9551043.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         1629 a          784 c          967 g         1637 t
ORIGIN      
        1 aatatacagc aggtaaagag gccagtaaaa ctgaaaactt cttttttgtc cattttatgt
       61 aatagaaaag aatgcttatc ataataaaaa aaggaatata aaaaacaaat tgaccgatcg
      121 cgatcatact attgcctccg ataaataaag tccatgatac aaaataacac tttgtgcgaa
      181 atggcgcaag cagttttgtg gatttatccg gtaaaatctg tatcgatttt gaaattagag
      241 gagaaacttg ctataatcaa agtgaaagaa ggagtgggat tttcatggat catgtagtgt
      301 tagaaatcga tttgcatttg atcaataata ttcgggtgat ttactatgtt gtaagtaaca
      361 gtgtcgaaca acgtgtactg acgaataaaa taaatggtat tttagcaaag aaagacgttc
      421 accgattcaa taatggtgaa ggaagttatt atagtatccc agtagaaaaa attatttata
      481 cgacagtcaa agtcagagaa gatctagata ccaaaaaagc atatgaacct atttttacaa
      541 cttattgatt gacagcagat ggaggaaaaa aatgaatcca tatgaacaac ttattcaaga
      601 tttgatcgat ggaaaaatcg aaaagattga tgtaaaaaga gaagaattaa tggcttttcg
      661 agaagcgtgg ctgaagttgg agaatcgtaa atatgtggtg ggaaaagcct cactgaacgg
      721 caatgtgact tatcattacg atccgacacg tttattttag tatgaagtat tcagaaaaga
      781 accaaaggag gacgatgcag cagcattgtc ctcctttgat tttacaaaag tgaaagtttt
      841 acttatttga atagattttt aaatttgttt ttgacactgt cgatatttac atcttttaaa
      901 agattttggg ctttctcttt ataaggtcct aattgttctt tatgttcttc gataaatcgt
      961 tttgcgccat cgaaattgcc gttcttaatc atttccttga ctttttgaaa taattcttct
     1021 tgattcattt tgaacacatc ctttgctatc aacatgattt tttacatgga caaaacgaga
     1081 aaaactgatt tatccacacg tcaataatat caaataacga tcatttttac taaaaaaaca
     1141 ctcgattctc gcttctaaag taggagaaaa atctccaact tgggacggat ggaatttctt
     1201 taactgaccc atatactgaa tatataacaa ggaggtagaa agatgaaagt gattaaatat
     1261 ttgattggac tggcggcgat tagtggtatt ttgctatttg gactgaaata ttatactgaa
     1321 ggaagttatg gagaaatccc tggtatactt gatcagctaa atccattagt tgaaaaagga
     1381 gaggtctatg taaagacaca aaaaccagag gaagtaaatg aatatggtac agcaaggtat
     1441 gtgcagaaag ctgctgatgc caatgggaaa gaacgaacgg ttgaatttaa tggtctttct
     1501 gtgttaaaag aaaatcatta tttgaaaatt agcaataaag gagcacacat agaaacgtat
     1561 gaggaagttc ctagagaaaa agttccggag aaagcattag caattattgg gtaaatcagt
     1621 ttttgtatta agaatacgcc cgattttatt attcataatt taattatgta aactaaatac
     1681 aagtagtaaa aataatacaa taatccccct gcaaaaagat tgttctttta cagggggttc
     1741 attttttcag caagcacatc aaattgggat ggcagggttc gaacctacaa ttgcttgatt
     1801 cagagtcaag tgccttacca gtttggctac atcccattta ttcaacaaaa taatcatagc
     1861 actttcctaa aaaaacgcaa gttatttttc ttgatcggat agttgttgct ttactcgtaa
     1921 aaggcggcgt actagtttct tttctaatcc agcaggaagg gcaatcagtt ctgctactga
     1981 caagacggtg tccaataact gatattcttt gacaggttca ttcataatat gatagatgtt
     2041 tgctaaacgt tcaatatatt caaagcgagt atactctgtt cgagcaatta gaaatttcaa
     2101 taaatcggtg gctttctcgt actcttgatc tgcgtaataa acaagagatt gacgatacaa
     2161 tttgatttcc agttcattga gggtagcagg tctgcgacgc tcgttccact cattaataga
     2221 gactttacct tgaacatttc ccttgcagtc acggacaaaa ataatcggaa tttcatttgg
     2281 atccattttt ttatgtgtga atttttccat tatctacgct cccttcaaag gtttggtatc
     2341 ttgagtataa gggatttttg tttgaattac tagcttttct ccaaagttag catctgtcca
     2401 cccattgcag tgaaaatcgc cgaaaaatcg ttgatcggta ctgctcgatt ttttacacca
     2461 tagggatcgt tgacataaat attttctttg tcatatccag tgactacaca agcatgaaca
     2521 agaggagtga ctcttacttc tccattattt gtggtccaag tcataaaatc agtttcatct
     2581 gggacttcaa aattgatagt cacagaagtc cagacaggat ttcctttaga tagtgctttt
     2641 ttgatatcat cgagttgagc atcggttttt ggaaaagtgt gataatccgt ttggacgatt
     2701 tgctgagcaa gggaggcaac tgggggaaca tagacgacca tagcccagtc tccgccactg
     2761 atatcaccaa caaatccttc attaggatcg ccatgagtcc catctgcatt gaaaacaggg
     2821 acgtaatcca attgctcagc taactggttt ttagttgttt cataaccata aaaagataaa
     2881 agcatagaaa gcgaagtgac ttcacagcca ttttctaaag aaggagaatc aaactggctc
     2941 tctaatggaa catctagtaa aacttctgct ttctcttttg tctttttcgt tttttgtgtt
     3001 gaactttttt gtgcagtgtt cattgtatgt tctggagaat ggatatgtag gtaataactt
     3061 gtaccgctta ataaaagagc agctgtgccc cctagaaaca atagtttttt gaacgttttt
     3121 ttcttcattt gaaatctccc ttttcagctg tttatctttc ctattataaa tgtattttta
     3181 ttggaaatgg aagtattata ttttaatatg taggaaataa ttagttttta aggaatatgt
     3241 tttaattttt tgaaaaaaat tcttctaaat acaacagctg ttttaattgt gaaaacgaaa
     3301 acagtacgct ataatgcctg taaggttgat ttttaactgt aaataaaaat caatgagtta
     3361 aatctccaaa gaaatagcag tggtaatgtg gagaaaagtt ccagcagttt aggaggattt
     3421 tgatgacgga agaaatgatt aatttaggag aacaatatgc atgcaaacca attggtttta
     3481 caaaaacggt cattggtgaa gttgtatcaa aaatgacaaa ctgcgccgtg gtcaaagttg
     3541 cccaatgtgc tgcagaggac caagaattat tagatgaaaa agcaagcatg gttgttgcaa
     3601 aatatgatac atttgaataa agaaaacaat tggacgtgcg gcgtgctgca agtccaattt
     3661 ctttgagaag gtaaatcagt attcttttcc tattttctgg agaagaaaaa gcgaggcgag
     3721 ttgtcatggt aacattcttt acggttctat tgtttttatt cgttgttgtg atgtttttat
     3781 ttctttggct ggttcgaaaa gaaattattt atcggactgt gcgaaatcga tgggtctact
     3841 tggtgatacc cttcttagtg tcactagtca tttggtatac actcatcagt catcctacag
     3901 cagatgaatt ggcaaaaggg atcttgtcag caatgatttt tattagtttt ttgttggaca
     3961 gtagaggaat cacagaagaa ggacttgttt tgaatagctt tgacaaaaaa ggtgttcctt
     4021 tttctgagat caataaaatt gtattatatc agccaaaagg ttctaaaatc gtaaaaatga
     4081 attttttccg aaacggatgg cgaggaccga tgctgaaatt ttctgcatca ttagaagaat
     4141 tagttccatt tttgtcccaa cgcttaaacg atgaggcaga gattgatatt atgattgatc
     4201 cagaataaaa acagaggttg cgacaagatg tttgtcacaa cctctgtttt ttatttcaaa
     4261 taaaattatc caaatacgcg gaagcgatcg gcatcgttca tgtattcttt ttccccagcc
     4321 ggtgtttctg gtgaaccgaa tactaattgc gaacgtagtt tccagtttga agggatattc
     4381 cattcttttg caactgcttc gtcaataact gggttgtaat gttgcaagtt tgcgcccaat
     4441 cctttatcaa ctaatgctac ccaagtatta gctgttgcga ttccgtttga ttgttctgac
     4501 caatctggga agttgtctgc atataattca aattgttctt gtagaccttt taccacgtca
     4561 gtatctttga agaataatac tgtaccataa ccatttttga aaccagctaa tttgttttgt
     4621 gtatttggga aagcttctgc tggagtcaat gggcgaagtg cttcttcagt gatttcccaa
     4681 agtttttcat gtgcatctcc gaaaaggata actgcgcgtg ttgattgagc attgaaagca
     4741 gttgggcttt ctttaattgc ttctttgatc aaagttgtta attcttcatt agaaagtgaa
     4801 acattacgtc ctagatgata gattgaacgg cggtttttca aagtgtcagt aaaagttgtc
     4861 ataataaaat ttctcctttg atttgcgttt tgcatttttt taattgatta cgaaatgaag
     4921 tatagcacct tacaaaaagt aagtaaacag atatgctcac aaaaaataag cgaagttcaa
     4981 aaaacaattt taataagaaa aatcgttatg taaggat
//
LOCUS       DACDSY010000164         4935 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDSY010000164 DACDSY010000000
VERSION     DACDSY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.167"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            31..453
                     /gene="mgsA"
                     /locus_tag="IWK44_002576"
     CDS             31..453
                     /gene="mgsA"
                     /locus_tag="IWK44_002576"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP9551044.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            776..1312
                     /locus_tag="IWK44_002577"
     CDS             776..1312
                     /locus_tag="IWK44_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9551045.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(1352..3115)
                     /locus_tag="IWK44_002578"
     CDS             complement(1352..3115)
                     /locus_tag="IWK44_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9551046.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(3118..4833)
                     /locus_tag="IWK44_002579"
     CDS             complement(3118..4833)
                     /locus_tag="IWK44_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9551047.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         1497 a          968 c         1000 g         1470 t
ORIGIN      
        1 atgttaacaa tatcaaaagg agtgtctctt atgaaaatcg ctttgatcgc tcatgaccga
       61 aaaaaaccta tgatggtgaa attagcaaca gcgtataaaa caatattgga aaaacatgaa
      121 ctttttgcta ccgggacgac tgggcagagg atcacggaag caaccggctt gccgatccat
      181 cggtttaaat ccggaccgtt aggcggggat cagcaaattg gagccatgat ttcggaggat
      241 cagttagatt tggtgatttt tcttcgagac ccgctggctg cacagcccca tgaaccagat
      301 gtaacagcac ttatccgttt atctgatgtc tatgagattc cgttagctac gaatatcgga
      361 acagctgagg tgctactaag aggactggaa gccggcttcc tagattttcg aagaatcgtc
      421 catgaaatag acaagcatcc actttcattt tgatggataa gcgtatggaa aaataacgtt
      481 taatgttcgc ctatatgtag ctataaaagc taaacaaact gataatatgc gatataatac
      541 gaacgtattg gataatataa acaataatat tagtttttcg tatttgcagt aaatgtgaaa
      601 atatgctata gtttctgtgg gaaaagatcc caaaaataat acagagtaga aaataaagcg
      661 ttaagtgcta agaggatggg atgttgcctc ttggacgaag gacgtaatgt tcgcggtttt
      721 attttcgcat tcgctgcatg atacgatgta gcagctctta aaggagatgc taaaaatgaa
      781 gaaacaccgg ttagacgcac gtatgattgc tattatgggc ttattgattg cattgatggt
      841 cactttgtcc cgactggtag caatcgagac cccatttatt aaaatcagtg tcacatttat
      901 tccacaagtg atcatgggca ttctttttgg tcctttttgg tcagggatag gcgcagtttt
      961 agctgattta gtaggaatgg ccctcttctc gaaatctgca ttctttattg gatttacatt
     1021 gaatgcattt attgaaggag caatctacgg attctttttc tatcgaaaag agatcacttg
     1081 gaaaaatgcc attctggcta cgcttagtgt cacgctgatc atcaacttat ttttgacacc
     1141 attatggctt gcgttgatgt accatgtgcc attgttcagc tgggtcgttt gggcgccccg
     1201 cctgttaaaa acagtaatat ggttaccgat tcaatcaatt gccatttatt acgtaggacg
     1261 tagtattcct tacaaaaaaa ttttaagaag tttagcgatc cacgctaaat aaaaaacaag
     1321 cagtcttccc atcccagaag actgcttgtt tttattgtgc tgtgaattgt gcttgataga
     1381 gccgtcggta ataaccatct ttttgaagca gctcatcatg ggtgcccatt tcgacaatag
     1441 ctccctgatc catcactaat atctggtccg catttttgat tgtggacagg cgatgggcaa
     1501 tcacaaaact ggtttttcct tccatcattg ctaagaaggc ttgttggatt tttttctctg
     1561 tcaatgtatc gactgagctg gtagcttcat ctaagatcaa cataggggga ttactgatca
     1621 tcgttcgagc aatcgtcaac agttggcgtt gtccatctga tatctttaac ccttgaccgc
     1681 caatcttagt atctaatttt tccggcaaac gttctacaaa ttcatacata taagaggctt
     1741 ttagggcatc attgatttct gcgtcacttg cttccggatt cccaaaacga agattttctc
     1801 gcaatgtact gtcaaatagc caagtatctt gtaacaccat tccaaaactt ttacgtaatg
     1861 tatcacgttg gatagagcgg atgtcatggt catcgactgt aattgttcct tttgttgtgt
     1921 cataaaaacg catcagcaaa ttaacaagtg tggattttcc ggcccctgtt tttcctacta
     1981 tagcaatggt ttgacctgga taagcagtaa aattgaaatc ttcgatcaaa cgctgcttgg
     2041 ggtcataaga aaaagaaaca tgatcaaaag agacttttcc tttgatattt ttgggttcca
     2101 aggcataggc atctggcaaa tcaggctttt cttctggctg atcgatcaaa tcaaatgctc
     2161 gttctagacc agaaaaagcg gtttgcagtt gtgtcgtgat tccagaaagt tcgataaatg
     2221 gtttagaaaa ctgacttgag tagatcgtaa agctggaaat cacaccaaca gtgattgacg
     2281 aaccactctt caatgccaat aggccgccaa ctaaaccaat cgataaataa gcaaggtgat
     2341 ctacaaaacg agaagacggg tttgtcaagg aagaagaaaa ttgtgcccgt tgtccttttt
     2401 catatagatc agcatttagc agttcaaatt gctgttgact agcttcttct tgctgaaaag
     2461 ctttcacgat tttctggtta ccgattcgtt cggtcacaaa gcccgagata tccccaataa
     2521 tcctttgctg tctatcgaaa tttgtttgtg aagcacgagc aacgatccaa ctcacaagga
     2581 aaataattgg tgtagaacaa agaacgacca atgtcagaag tggacttaga cgcatcataa
     2641 aaataaacga aagaatgatg attgccactc cagaaaataa ttgattgaag gccgcagaaa
     2701 ttgcaatgga aatattgtcc atatcatttg taaatcgact gacgatattt ccgtgggagt
     2761 tttgatcata ataacgaagt ggcaatcgat tcagatgagc aaatgcatct tttcttaatt
     2821 ttgcaacgga taagtaagct aaccgattgc ttaacacttg gatgagccat tgactgacta
     2881 cgttgattaa aacgatacca ccgaatagaa taagtaaatg gaacagtcga ttgaagcgga
     2941 cctgtccttc tccaatcatt tgatcgatac tttgaccaat ttgtaacgtc atcacaactg
     3001 ttgcaatccc attgatgatt cctagtatca cagctagcca aatgtctttt gcataggcag
     3061 taaggtaagg aatgaatcgg cggagcgtct gcaaggataa tttttttact ttcataacta
     3121 ttcttcctcc tgtgatgcga cgatttcctg atattctgct gattgagcaa gtaattcttc
     3181 atgggtaccc tgagcagcaa tcttaccttg atccatcact aagatatgat gtgcttcttg
     3241 aatagagcgg acacgttggg agataataat cactgtactg ccaagataag tctgtagcgc
     3301 cttacgcaaa tccaagtctg tttgataatc caacgcactt aaagagtcat ctaaaatcag
     3361 taatgctggc ttggcaatca aagctcttgc aatcgtcagt cgctgttttt gtccgccaga
     3421 gaaatttttc ccattttcca tgacttttgt atctaaacca tttggaagct gacggacaaa
     3481 ttcttctgcc tgagcgattt tcagagcttc ccaacattct ttttctgtgg cgtctttctt
     3541 accccattga aggttttcac gaatagtccc ggtaaatagt acagaagttt ggggaacaag
     3601 agcaatttgc tgacggagat gatccagctg ccaagaacga acatccattc cgtccattat
     3661 aactgtaccg gaactgacat catagaaacg cggaatcaat tgtgtcaaag tcgttttccc
     3721 gcttccggtt ggaccaatga ttccaaggac tgtatttcgc ttcagctgaa atgtaatatt
     3781 ttggagagaa agaccagaat caggagtata acggaagtca acatggtcaa aagcgataat
     3841 tgcttcagaa gaaaaatcgg gagtagtact gacctcagct tcaagaatcg aattttctgt
     3901 gtctagcacc tctttcaccc gatttcctga agcttcggca cgtgtgaaga tcacgacgag
     3961 gtttgaaaca acgatcagtg ccaaaagcat ttgattcata taattgatca aagcaagaac
     4021 ttgtccttgt tgcaagctac caatatttac tttgatcccg ccaactgtca gcaaacagat
     4081 gatcccgaca ttcatgatca aggttgtagc aggtgataat agggcagaga tattggaaac
     4141 acgtaaataa ttgttcgcta gatcatttgt gcttttatcg aacttctctg tttctttttt
     4201 tgtctgcgca aatgcgcgga tgacacgaac accgctaaga ttctgactaa cgtgttcatt
     4261 gagctgatct agtttttctt gcactttttg gtagagagga acggtttttt tgatgataaa
     4321 aaataaaatg attgagaaaa taggcaataa tgctaagaaa aacagaccta cttcccagtc
     4381 gatcacaaaa gccatgacca ctgacccgat acttaaaaac ggtgctctga tcaataaacg
     4441 aatgaccatt gctaaagcta gctgaagctg attgatgtcg tttgtcattc gggtgatcaa
     4501 ggtatctgtt ccaaaatgat tcaactcgtt gtgagagaaa gtattgattt ttttgattag
     4561 ctgattacgt aactctgtac caaatccttg tgaagctact gaagcatagt attggcaaat
     4621 gatagcacaa atcagaccaa tcacagacat agcgaccatc cataagccca tttcaatgat
     4681 ttttcctcgg tcattcattt ttagtccatt atcgatcaag gaagccatca ttaaaggcag
     4741 gacaagttcg aagacagctt caagaaattt gaagaacggg cccaaaatga tttgcttacg
     4801 atagtctttt gcataacgta ataactgaat catatgattc ctcctattat ttatttgtcc
     4861 acaattgaaa aaacaagtac taccacattg tagcttagga aatttagaat gaaaagagaa
     4921 caagcctttt cataa
//
LOCUS       DACDSY010000165         4869 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDSY010000165 DACDSY010000000
VERSION     DACDSY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.168"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            144..944
                     /locus_tag="IWK44_002580"
     CDS             144..944
                     /locus_tag="IWK44_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9551048.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            963..2831
                     /locus_tag="IWK44_002581"
     CDS             963..2831
                     /locus_tag="IWK44_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9551049.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            2824..3315
                     /locus_tag="IWK44_002582"
     CDS             2824..3315
                     /locus_tag="IWK44_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP9551050.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            3303..3857
                     /gene="srlA"
                     /locus_tag="IWK44_002583"
     CDS             3303..3857
                     /gene="srlA"
                     /locus_tag="IWK44_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP9551051.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            3875..>4869
                     /locus_tag="IWK44_002584"
     CDS             3875..>4869
                     /locus_tag="IWK44_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP9551052.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1694 a          762 c         1026 g         1387 t
ORIGIN      
        1 gcaatctatg caaaaagatg aaaatttttt ttgctcttcc attagtacac attttttagc
       61 tgaagctgta aatttcatgt cttaattttt tttagtgagc ttttatactg gatttgtaag
      121 aaaaaagaag gagaggatca catatgtcgg attggttgga tattgcagga aaagtagtaa
      181 ttgtaactgg aggttcctca ggaattggaa gaagtatcgt cgaaaatttg ttgaagcaga
      241 atgcacaagt agcgaatttt gatgtaactg aatgccgtat acagcatgaa aacctactat
      301 ctttaaaagt cgatgtgtct tctaaaacgg acataaaaga agggatatat aaagtaatga
      361 aacattttaa gacgatcgat ggtctagtca ataatgcagg aataaatatt ccttctcttc
      421 taatagatag aaatcatcca aaaagcaagt atgaattatc agaacaagtt tttgataaga
      481 tgatcgctgt caatcaaaaa agtgtatatt tgatgagcca agcagttgga agaatcttgg
      541 tgcaaaaggg atcaggagtg atcgtgaatc tttcttctga atcaggattg gaaggatcag
      601 aaggacagag ctgttatgct gctacaaaag cagcgatgaa cagttttact cgttcttggg
      661 caaaagagtt aggaaagagg aatgttcgtg tggtaggtgt tgcaccaggg atactggaag
      721 aaaccggttt gagaacacaa gaatatgaag aagctctttc ttatacgaga ggtataagcg
      781 tcgaacagct gagaaacggt tacagtcgaa caagtacgat ccctctagga aggagtggta
      841 agctgcaaga agtggcggat ctagtctgct attatctttc agaaagatca agttatatca
      901 ctggagtcac gactaatatt tcaggaggta aaacaagagg ataaaggata ggggggacgc
      961 acgtgacgct agtaaacaga tggtaccaat tattgaaatt actagtcact cacaagaaag
     1021 tttcaatcca ggaagtgcaa aaagaaatca aaaccagtaa tcaaaccata aaaaaagata
     1081 ttggggagtt gaatgaagaa attgccggta ttgcaaaaat catccaaaaa aacaaacatt
     1141 ttgaacttga aatttatgat tttgattttt ttgatgagat catgcaagga aaattgaaaa
     1201 cagccagtga tttcaattcg gcatccaaaa gaatcgctta tttactaaat aggctgatcg
     1261 aaacagagga tttcttacgt atggatgatt tgtctgaaga agtcggtgtc agcagaggaa
     1321 cagtggtcaa ggatctaaag acattaaaag agatgatcaa tgattttgat gtaaagatta
     1381 caggcacacc aaataaagga ctaagaataa taggagatga gctagaatta cgactacttt
     1441 tattttattt tgtttctccc tacttttcag agacgaaaac agaacagttt ctagaagaaa
     1501 aacaagcaat caaacatttt cagaagaaga cgaatgctag taaacaagaa atcacccttt
     1561 taacgaaagt tatcggtatt tctttgaata gaatctctag taactatctg ttgaagaagc
     1621 ccatcaatcg ttacagtggg tgttttgaat atatccaaga attcaatgaa ctgatcaatc
     1681 agttggaaga aatctatgaa ctaactttga gccaatatga aaaagatttt atcagttttc
     1741 ctttgaacat catcaacaca agtataacga tgataaggaa agctaactct gatttgcgct
     1801 tatatttcga ccccatgatg atgcgtattc gacgattaga tctcattgat ctggatgagt
     1861 ccttcttata tcaagagatg aaacagcacc tctctttatt gataaatcgg gtaatttttc
     1921 gttatcgatt gactgattta ttttatggtg agatagaaaa aaagtatcct ttttcttacc
     1981 aaatcgctag cgaatgcata caagcattag aagctatctt gcaacataag atctctaaag
     2041 tagagatcag ctacctaacg ttgtatttgg agttagcatt tcgatatcaa aaagagcatt
     2101 tttcctctaa aaaagtagcc attgttcata cgacaggaaa aggaacagca ttaatgatcc
     2161 aaagacaaat caaaaggata ttggggaggg atatacaaat aacacatgtt tcagaagaac
     2221 aatatgaaaa agtagatcta aacaaatatt tcgctgtttt tacaacgatt ccgttaaaaa
     2281 atgtaaatga agagacaccg gtgatccatt tgactaatct ttttgatgac tcttggttaa
     2341 gggcacaatg gcaaaaagca aaaaaaaagc gattagccgg gtttcattct actgtttttt
     2401 tctatcaaat atggaaagaa aaaaagacgt atcaagaata tttaaacaaa ttagtcgctc
     2461 ctttgatcag aggctattat gtggatgaag aatttgaaaa aaggatcttg aaaagagaat
     2521 atcagcaaag cacatttgtt aacaaagaat ttgcttttcc tcacacaacc aaccagctat
     2581 ctcaacacat tgttttatca gtagggctaa taactgatca aggtttagca gaaacaggag
     2641 gaaatggagt tattttttta tttgccatac ctgaagaaat gacggctgaa gttgagaatg
     2701 aattatttga gctatacgat atcctatttg aactcataaa aatgggaata gatgagttta
     2761 ttgaattagc accgacacaa gaatcatttt taaaactaat caaaagtaaa ggagagagac
     2821 ttcatgaatg atatgtatct attcatagtt ttcgcagctt cagcttatgt gctgcaagca
     2881 gttatcagtt ggaagcaact aaagcatttc aatcaaacat atagcgaact gcgaagaaaa
     2941 ggaaaagtag cgattggtaa acgacctggg aaaatccgtt caggcacgat tgttatgcta
     3001 gctatagacg atcatgatca agttttagaa gtcagaaaaa tgcaaggggt tacatctttg
     3061 gcaaaatttc attcattacc tgtctatgcg ggtgaagata tccattacat cgatcgttac
     3121 catccctcag ttcgagcgga agatcgtttg acacaagcag ctttagaaaa tgccagagaa
     3181 gtatacttac gaatagaggc aggaaatgaa aaaagtgaac aaccttcatt gccttttatt
     3241 gctatgctca ataattttcg gatatggaaa agtacgatca aagaaaaaag gagtgacaaa
     3301 cgatggatat cataacaaaa tttgctgagg gatttatgag gctgttccaa acaggtgcag
     3361 aaacattcat aagctggatg acaggaatcg taccagttgt cttgatgctg atggttgcaa
     3421 tgaatacgct gattgctttt ttaggtgaag accgagtaac gaaagtagcc aaattatcag
     3481 cgaaaaatcc gcttactcgt taccttgtgt taccgttctt atctgctttt atgttgggaa
     3541 atcctatgtc atttacaatg gctcgtttcc ttccagaata ctacaaacca agctattatg
     3601 ctgctcaagc tcaattttgt catacgagca atggtgtatt cccccatatt aatcctggag
     3661 aattgtttgt atggatgggg attgctcaag ggatcgaaac cttagggcta aattcgatgg
     3721 agctggctat ccgatacttg cttgtgggac tgctgatgaa ctttattggt ggttggataa
     3781 ctgattttac tactggattt gtatgcagac agcaaggaat cgttcttagc aaaaaagtag
     3841 agctttcagt tgattagaga aaggagagtt ttaaatgtat ggtgatatta agatcgtaag
     3901 aggaaacggc gggtatggag gtcctttgat tattaggccg acaaaagata aacacaagtt
     3961 catctatgtc acaggtggag gtgagaggcc aatgatcgta gataaaatag aagaactgac
     4021 aggtatggag tctatcaatg ggttcaaaac atcaatacct gatagtgaaa tcgcgcttgc
     4081 aatcgttgac tgtggaggga ctcttagatg tggaatttat cctaaaaaag ggataccaac
     4141 catcaatatc gttcccacag gaaaaagtgg cccattagca caatatatca ctgaagatat
     4201 ttatgtttca gcagtagggt tagaacaaat acagccacta atggaaactg atgataacaa
     4261 agaaacagta aatactgcat ctccagataa aaagtacaaa tatgatactt ctaaaaagat
     4321 aaccgagcaa aaagcagagc agcaaaactg gatagcaaaa attggaatgg gcgcgggaaa
     4381 agtagttgct acattcaatc aatcagcaag agaagcagta gatacgatga tccggacagt
     4441 gatccctttt atggctttcg tttccctttt gattggtgtg atacaaggtt ctgggatcgg
     4501 taatgtattt gctaacatta tggcaccttt agcaggaaat gcagttggat taacaatcat
     4561 tggctttatt tgttcactgc catttttatc tcctttgctt ggtccagggg cagttattag
     4621 ccaagtgatt ggaacattga tcggtgtaga gattgggaaa ggaaatattc ctccccaatt
     4681 agcattaccg gcactatttg ctatcaatac tcaaaatgca tgtgatttta ttccagtcgg
     4741 tctaggtttg gaagaagcaa aagctgagac agtagaagta ggtgtaccat cagttcttta
     4801 ttcaagattt cttaacggca tcccacgagt tatcattgct tgggtagcaa gttttggatt
     4861 atatagctg
//
LOCUS       DACDSY010000166         4852 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDSY010000166 DACDSY010000000
VERSION     DACDSY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.169"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(7..1164)
                     /locus_tag="IWK44_002585"
     CDS             complement(7..1164)
                     /locus_tag="IWK44_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551053.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1167..1523)
                     /locus_tag="IWK44_002586"
     CDS             complement(1167..1523)
                     /locus_tag="IWK44_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551054.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="IWK44_002587"
     CDS             complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="IWK44_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP9551055.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2991..3830)
                     /locus_tag="IWK44_002588"
     CDS             complement(2991..3830)
                     /locus_tag="IWK44_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9551056.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4015..>4852
                     /locus_tag="IWK44_002589"
     CDS             4015..>4852
                     /locus_tag="IWK44_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP9551057.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMK"
BASE COUNT         1573 a          966 c          718 g         1595 t
ORIGIN      
        1 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
       61 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
      121 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
      181 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
      241 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
      301 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
      361 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
      421 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
      481 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
      541 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
      601 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
      661 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      721 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      781 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      841 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      901 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      961 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
     1021 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
     1081 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
     1141 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
     1201 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
     1261 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
     1321 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
     1381 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
     1441 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
     1501 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
     1561 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1621 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1681 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1741 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1801 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1861 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1921 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1981 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     2041 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     2101 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     2161 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca
     2221 ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc
     2281 gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta
     2341 tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc
     2401 gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca
     2461 atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct
     2521 tttaaataaa cttccccaat aactttttct gaacttccat taccatccca accatatgtt
     2581 agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg
     2641 acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca
     2701 tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca
     2761 agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc
     2821 ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg
     2881 aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa
     2941 tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa
     3001 acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc
     3061 aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta
     3121 attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg
     3181 actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc
     3241 gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata
     3301 ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca
     3361 gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca
     3421 cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc
     3481 ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta
     3541 gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct
     3601 tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga
     3661 aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat
     3721 tcactatttc caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa
     3781 ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc
     3841 tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt
     3901 tctccatcat agagagaata gatttttacg aactaagaat atctacagca accttacgaa
     3961 aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga
     4021 aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc
     4081 tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt
     4141 caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg
     4201 attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca
     4261 aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca
     4321 gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac
     4381 ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc
     4441 tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca
     4501 aaaaaagaag caaatgaagg ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat
     4561 aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct
     4621 actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct
     4681 gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag
     4741 gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca
     4801 aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa aa
//
LOCUS       DACDSY010000167         4820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDSY010000167 DACDSY010000000
VERSION     DACDSY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.170"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..675
                     /locus_tag="IWK44_002590"
     CDS             <1..675
                     /locus_tag="IWK44_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP9551058.1"
                     /translation="LSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQGMLEL
                     LGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRMI
                     FAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFGQ
                     VLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKKY
                     YPGKGQ"
     gene            677..1621
                     /locus_tag="IWK44_002591"
     CDS             677..1621
                     /locus_tag="IWK44_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP9551059.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1820..2464
                     /gene="pcp"
                     /locus_tag="IWK44_002592"
     CDS             1820..2464
                     /gene="pcp"
                     /locus_tag="IWK44_002592"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP9551060.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2543..3820
                     /locus_tag="IWK44_002593"
     CDS             2543..3820
                     /locus_tag="IWK44_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551061.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(4004..4753)
                     /locus_tag="IWK44_002594"
     CDS             complement(4004..4753)
                     /locus_tag="IWK44_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP9551062.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         1546 a          861 c         1043 g         1370 t
ORIGIN      
        1 cttagccttt taggcgtctt gattgttatt ctcgggtttg catttaaatt agactcgata
       61 ttgatcgtaa tggttgcttt agttgttaca gctttgactg gcggtcttgg cattcaagga
      121 atgttagaac ttttaggtac aagttttgta aacaatcgcg ggatggcgat ttttatcatc
      181 attatgttgg caacaggaac gcttgaaaga aatggtctga aagaatcagc ggcttccttg
      241 atcaagcgat tcaagaaagt atctgctggt atgattgtag atatttatgg gatctttcga
      301 atgatctttg cggcttttaa cgtgagtttt ggtggagttg ctggatttgt tcgtccaatc
      361 attttaccga tggcattggg gaccatcgaa tcaaaagggt tagaagtcca tcctaagcat
      421 gaagaagaac taaaaggtat ggcttcagct atggaaaatg tctgctggtt ctttggccaa
      481 gtactattca tcggaggagc aggggcgttg ctagtccaat caacactgaa agatctagga
      541 tatgacgtaa cattgggaca gcttgcttta gtggaagttc cagtggctat cacggcttta
      601 gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat tagcgaagaa atattatcca
      661 ggaaaggggc aataagatgg acttttttac aaatcctgat attttattag gcgataaatt
      721 actggagatc ctttatatcg tgatgggact cgtattgatt tacactggag taagaaattt
      781 aacagataaa accaatcctc atcgttatgg gtcagccttt ttctggacgg tcttaggggt
      841 agttattgca ggaggaagat ggcttccggc aatcgtgaac ggtggcttga tctttgcgat
      901 gaccatacca gcaattgcta agaaagtcag caaaggagaa agccggcttc catcaaaaga
      961 atatatggaa aaaatgtcag acaaactagg aatgaaaatt tttattccag ctttgagtat
     1021 cggtgtcttt gcgatcttgt ttgctctatt taccaattta ggtgctttag tcggcgtggg
     1081 cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt tctcgagata atcgaccaag
     1141 tacattttta gatgatgcag cagatatgct gggcacagta gggccgttaa gcatgttgcc
     1201 aatgctttta gcttctctag gtgcggtatt tacaagcgct ggagttggga cagtgatttc
     1261 caatgcagta ggtacgatcg ttccagaagg aaatgtgaac atcggcatca tcatttacgc
     1321 tcttggcatg gtgatattta cggtaatcat ggggaatgca tttgcggcca tcaccgtgat
     1381 gacggttggg atcggtgcac cattcgtatt tagtcatgga gcgaatccag cgttaatagg
     1441 aatggttgca ttgacttgcg gtttttgtgg gaccctgctg acaccgatgg ctgctaactt
     1501 caatatcgtg cctgtagcga tgctggagat gaaagacaaa tacggagtga tcaaaaacca
     1561 gttgtttatt gcgttattta tgctagtttt ccaaattgct tatatgattc tatttaaata
     1621 atagaagaga ggggttgtga cagaataatt gtcgcaacct cttctaacat tgtagtggaa
     1681 cactattttt ttataagaat atattaagtt tgatctgtta tgttatttaa actattgttt
     1741 cctttattta cgctgttcaa ggaaaatggt atgataaaag ataaccatat gttaaatcaa
     1801 tcggacggag gaacaacaga tgaaagtact agtaacaggt tttgatccat ttggtgggga
     1861 caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca gatgaaatca gcggtgctga
     1921 aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc agtcaagtag tcaaagaagc
     1981 catccaacag caccaaccag atgtcgtgat ctgtgtcgga caagcaggtg ggcggtcagc
     2041 agtctcgttc gaacgtgtcg caatcaatct agcagaagca agaatccctg ataatgaagg
     2101 aaatcagcca ttcgatacgg ctttagaaga aaacggtcca gccgcttact ttacctcatt
     2161 gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt ctaccagcgt acatctctta
     2221 tacagcagga acatttgtct gcaatgatat catgtatcgt ttgcttcatt tgatcgaaac
     2281 acaattcccg gcaattcgag gtgggttcat ccacgtgcca ttctcacccg atcaagtgat
     2341 cgatcgtcca gtgggtacgc cttctatgtc tttagaagac attgcttctt ctttgactta
     2401 tgcagttgaa gcagcagtag aaaataaaga agatatttcc ggaaatgccg gaacaactca
     2461 ctaatttagc aagaaacact ggctggttgc taacgttatg ttagtagcca gctttttgta
     2521 attagaaagg aaagaaacag ccgtggaaga atggcaatcc gtctttgaag aatggtttcc
     2581 aaaagaaatc agcaaaagct atcccatcaa aatttctaaa caatacacaa gcagtcaacg
     2641 ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa ctagtcgata agcaccgccg
     2701 atatctaatc agctctcgtt ttatggaaga acattattta gctgcaacag attgggtgtt
     2761 ttctgacttc aagatcaatc cgttttttcg aacaaaacga agccagcaaa aattgtattg
     2821 tgagtgtggt agagaattaa aagtgcaata catcgttaaa tcgccaaaaa ccggaaagat
     2881 cctaaaacta ggcatcaacc actttgccga tcacctgcat gtctctccga cagtggcagc
     2941 ttcgatccat cagggaatga caaaagttga tctggcctta gacgaattat tatggcttaa
     3001 gcagaagaat atcgattttc ctgaaggact atggcaaaaa tactgttttg tcttgtacca
     3061 aaatcgtcgg atgaaacagc catatttacc ggatataaaa ttagctcagc ggctcgcaga
     3121 atttcgccaa gtagaaatgc cgatttacat agcggattat caagcattgg aaaatgaaat
     3181 caagaaaata agtgaacaca tcaatgggca gtcaaaaaag cgacagataa aaaaagaact
     3241 atttgacgac tttgcagaag aattagtaaa ggatgtagaa gaattcttga tcaactatcg
     3301 cgcattttta cgtaaagatt ggcaatccat cgtatatgaa gaagtgccag tacatcctaa
     3361 tgcttatttt gaaacattta tttccgtgtt gagaaagaca aaacgtcaac gaacaccaga
     3421 agtcactgct cagatggaat attttgcgaa aaaccaacga ttcatccaac caaaaatcta
     3481 tctatttatt tggaagcaat attgtcgcta tggttttaca gaagggttct tcgacagcat
     3541 tcctcggata gtcagaaatg gttttttaaa agtattgcgt aaagaacgag aagctataca
     3601 atctgctgat aagaaagacc gtacagtctc aaaagaaaaa tggcaactag tagtgaaaga
     3661 tatccaatct ggaaatgtgc aagagacaat cgacaaatgg aaaggaaaac attatcgttt
     3721 tactgaagct cagaaacaag cgttggaata ttatcagaag ttagaagaat cactgcgttt
     3781 caatgatgag gcaagaaagt atctgaagga attactataa aaaatagagg ctgggacaga
     3841 agcgtttaac tccaagaaat aagaagaaat tcacgaaaat tgcttttcaa atttttgtga
     3901 atttcagctt atttccgaag gagttgcttc tgattccgcc gtttatacgt gtttagagcg
     3961 tgagacaaaa gtgttcttta cttttgtccc acgctcagtt tttttatgcg tgttgttgtg
     4021 cgttgtagta ttcttcttcc attttttgca tacgttcacg ttctttttgg ttataccaag
     4081 gtgaaacggt aaatgcaagg acatcattca gcaagtaaac agagctgttc acgaacatcg
     4141 ccaaacttgc agatccatgc gtaaatgaaa tataccaaag gaccatttga gcaagccctg
     4201 aagctaacca ccagtaatat tggttgttgt aacgcaagaa actgatgata ccagcagtca
     4261 agctaataga aaaagcaatg gcatctaccc acggccgagg atcatctgta taacgaccaa
     4321 tcaggaatcc tgaaacaaga tagacaagga aagtactgat gattgcgata aaccagcttt
     4381 ttccagtgaa ttttcggatt tttgaagcca tattatgatt ccattcttta cttaacaata
     4441 cggggatatc caatgtgatc atataagcaa tctgttcacc gatacttaaa tagttctttg
     4501 ctgaaaaccc aacgataata aaacaaatcg cagacaaaat acctaaaata ccgttgactg
     4561 attttgccgc attgatggat aaaatacaca atacaccaag cgtggtaccg ataaatgtaa
     4621 taacggacag ccaagtgatg ggactattga caagagtcat gacctggcag ccaagactga
     4681 aaaagaaaag ataataattt tgttgcggcc agccttttag ctgatgagct aaaaactgaa
     4741 aatatgactt catgtatgag gactccttaa gacactaaat gtagtgtgtt ctgtatttac
     4801 taacacaatc tattgtatgc
//
LOCUS       DACDSY010000168         4795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDSY010000168 DACDSY010000000
VERSION     DACDSY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.171"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..258)
                     /locus_tag="IWK44_002595"
     CDS             complement(<1..258)
                     /locus_tag="IWK44_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551063.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="IWK44_002596"
     CDS             complement(274..924)
                     /locus_tag="IWK44_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551064.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="IWK44_002597"
     CDS             complement(935..2107)
                     /locus_tag="IWK44_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP9551065.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="IWK44_002598"
     CDS             complement(2126..2998)
                     /locus_tag="IWK44_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9551066.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="IWK44_002599"
     CDS             complement(3020..3940)
                     /locus_tag="IWK44_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551067.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="IWK44_002600"
     CDS             complement(3940..4209)
                     /locus_tag="IWK44_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551068.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="IWK44_002601"
     CDS             complement(4206..4586)
                     /locus_tag="IWK44_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP9551069.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         1254 a         1052 c          791 g         1698 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatg
//
LOCUS       DACDSY010000169         4788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDSY010000169 DACDSY010000000
VERSION     DACDSY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.172"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            564..989
                     /locus_tag="IWK44_002602"
     CDS             564..989
                     /locus_tag="IWK44_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551070.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            2141..3118
                     /locus_tag="IWK44_002603"
     CDS             2141..3118
                     /locus_tag="IWK44_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9551071.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="IWK44_002604"
     CDS             3128..3625
                     /locus_tag="IWK44_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9551072.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="IWK44_002605"
     CDS             4001..4282
                     /locus_tag="IWK44_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551073.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="IWK44_002606"
     CDS             4284..4700
                     /locus_tag="IWK44_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551074.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1757 a          816 c          786 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DACDSY010000170         4773 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDSY010000170 DACDSY010000000
VERSION     DACDSY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.173"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..196)
                     /locus_tag="IWK44_002607"
     rRNA            complement(<1..196)
                     /locus_tag="IWK44_002607"
                     /product="16S ribosomal RNA"
     gene            116..259
                     /locus_tag="IWK44_002608"
     CDS             116..259
                     /locus_tag="IWK44_002608"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551075.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            complement(882..2387)
                     /locus_tag="IWK44_002609"
     CDS             complement(882..2387)
                     /locus_tag="IWK44_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9551076.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2384..3070)
                     /locus_tag="IWK44_002610"
     CDS             complement(2384..3070)
                     /locus_tag="IWK44_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9551077.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(3263..4684)
                     /gene="gndA"
                     /locus_tag="IWK44_002611"
     CDS             complement(3263..4684)
                     /gene="gndA"
                     /locus_tag="IWK44_002611"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP9551078.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1317 a          996 c          800 g         1660 t
ORIGIN      
        1 gattgttata cggtattagc acctgtttcc aagtgttatc cccttctgat gggcaggtta
       61 cccacgtgtt actcacccgt tcgccactcc tctttttccg gtggagcaag ctccggtgga
      121 aaaagaagcg ttcgacttgc atgtattagg cacgccgcca gcgttcgtcc tgagccagga
      181 tcaaactctc ataaaaagtt cgaacaatct tgcgattgtt aagctcaatt tgtttgctag
      241 catattgctt gcttgttaaa aatgtttgtt gtcttgaatt gaatcaagac gccctacaca
      301 tttggtttgt cttactttgt tcagttttca aaggtctaat ctttttgttt taacaactct
      361 ttaatcttat cagattcaga agagaatgtc aacaacttct ttgtttattt tttctaaacg
      421 tctttgccac tcattagcag cgacttgatt atcataccat ctttattgtt atttgtcaat
      481 aactttttcg aagttttttt cgataaaatc aagctttatt agctatctca tccaaacgac
      541 ttcgttatta taacatgaca tcaaaagtgc gtcaacacta ttttttataa tttttagtga
      601 caccttaaag gaatcattta gaacaaataa taatatagca atctctttat aaaattgcaa
      661 tagaaaattc cctattttta tttattattt ttaaacacga atattacagc attttattgt
      721 tttttcgttc atctaaaaga tacttacatt taattaacgg atgttattct atttaattgt
      781 tcgctagttt ttttataatg acatccctga atttctcact tgcttgtatt ttgtacctta
      841 ttctttgttg gatatataaa aaaacaagta aagcacatgc tttacttgtc ttctgccttt
      901 gcgataggaa taaagattct aaaaattgtc ccttgcccta ctacactttc tgctaagata
      961 cgtcctttat agttttcaac cagttgttta gcgatagaca accccagacc atttccaccc
     1021 ttcgttctag ctcttgcttt atccactcga taaaaacgat tgaagatctt attaaggtct
     1081 tctttgggta ttccttcgcc aaagtcctga atcgcgatct caaattcatt cagagtggaa
     1141 gaaatagaga tatggacttc ttttcgtgta gttgaatact ttactgcgtt atccaatatg
     1201 ataatgatta attgttcaaa gtgattacga taaatttgca gggtcacttc ttttgtcaaa
     1261 tcatcatcta aagtgatcac atagtctgga tataatatct tgaaattatt ataaacttga
     1321 taggtgactt cttttccttt agttgtttca ttcgcataat gaacatctac ttgttctgct
     1381 cttgataagt ctagcatctc ttgaactaag cttttcatac ggctgatttc ttgcatgctg
     1441 gcgcttaatg attcttctaa tatctcagga tcatctttcc cccaacgatt tagcagacta
     1501 agatgccctt caataattgc aacaggtgtc cttaactcat gagatacgtc ttccacgaat
     1561 tgctcttgtt gttcaatata caaacgcata cggtcaagca tctcattgaa tatttcagct
     1621 aaatctgcca attcatcatt tgtgtcgatt tcaggcatgt ggatatctga ttgcggatct
     1681 ttacgtatag tatccattgt atctctgagt accttgagtg gttttaagaa ataagaagat
     1741 aaaaagaaac ctaaaatact acttaagatc aaagaaacaa cttctaaaac aataagagtc
     1801 aataatagct tatttcgaat atcatagaaa gaagataatt catagaagct ttgcgcatag
     1861 ccgatctttt ctcttgtacc tttagaatat ataggctgga tagataaaaa gccagttttt
     1921 ccttctagtg taacgatcgt tggaacttta cgtgtagtct gaatcaggtt tcgactgttt
     1981 tcatgggttt taaaaatcaa gttctcgtcc aaatcataaa cataaagatc cattgatggc
     2041 tgccctagct cagctataaa ggaatcgatc ttcattaagt ttccttcgac cagtgaagta
     2101 tgatctacga cctcatctcc gtcatttgtc gctttcgtca gattacgata cgtatttact
     2161 aatgtcagtt cttcatccga gttagccaaa cgagaagcta cttctgaaat cgtacgctct
     2221 acattgtttc tttcttttgc cacaatcaga ttgatagatg atttataggt aatgacagcg
     2281 aaaattgtaa acacaacgaa tatgaagaaa gaactcgcaa aggcccactt gattgtcaag
     2341 gagggccctt tcagttctct tttttccgat tttttcggct ttatcacgag cgcatcacat
     2401 aacctgttcc gcgtacagtt tggatatagc tttcctcccc gggaacgtca attttgttgc
     2461 gaagataacg aatatatacg tccactacgt ttgtttcaac ttctgtttcg tatccccaca
     2521 ctttatttag taaaacgtca cgtgctaata ccacgttcac gttttccatt aaagtaagca
     2581 gcaactcgta ttctcttttt gttagttcaa ttacttcatt tccgcgacgt acgacacggt
     2641 tttctttttc gatagtcaaa tcgcgataag taatagtagt ttgtttagcc acatttttat
     2701 cgccttcaat atcgattcga cgcaacaatg cacgaagacg tgctaataat tcttcgatag
     2761 caaatggttt gacgatgtag tcatctgctc cgtggtcaag tccagataca cgatcgatca
     2821 cagaatctct tgcagtcatc ataatgattg gtgtattttt gacttggcgt acacgtctgc
     2881 agacttctaa accgttcaat tctgggagca tcagatccaa caatatggca tcccactcat
     2941 tatttaatgc cgcatctaaa ccagttcggc cgttgtagtg gacttctgcg ttataccctt
     3001 catgcttcaa ctctagctcg acaaatcgtg ccaagttctt ttcatcttca atgattagaa
     3061 tattgctcat ttattatttt atcctttcta ttctactggc tttttaaaaa ctctatcatt
     3121 ataccgataa tatgcttaaa aatctatagt cattttatca tggatagcaa ttttaaaaaa
     3181 gagctgaagt ctactcataa caatcgactt cagctctttc gtcctctttt tattttctca
     3241 tttcctaaca gccgaaagac tattattctt catggtacca tgagtaatgg aagattcctt
     3301 ctttgtcgac acgctcatag gtatgtgcgc caaagtaatc gcgttgggct tggattatat
     3361 ttgcaggcag acgttctgct ctgtatgaat cgaagtaagc gatggctgat gagaaagtcg
     3421 gtacaggtac gccagcttgg acagccaagg caacgacgtc tctgactgat tgctgatatt
     3481 ttttcgtgat ttcaacgaag taatcatcta gcaataaatt ttcgatctca ggatttttgt
     3541 cataggcatc cgtaattttt tgcaagaatc gagctcggat gatacatcca gcgcgccaga
     3601 tttttgcgat ttcaccaaat ggcaagtccc aatcaaattc ttttgaagcg acacgtaatt
     3661 gagcaaatcc ttgggcataa ctcatgattt tactgaaata caacgcttct cggatttttt
     3721 cgaccaattc ttttttgtct cccgtgaact caaaatcgtt cgtacgagac aaaatcttgc
     3781 ttgcttgtac gcgttcttct ttataagcag aaatatagcg ggcaaataca gattcagtga
     3841 tcaatggaag tggcacacct aagtcaagcg cgctttggct tgtccattta cctgttcctt
     3901 tattcccagc tgcatcaagg atcacatcta ctactggttt acctgttcct tcatcgtctt
     3961 tacgtgtcag aatgtcagca gtgatttcta tcaaatagct gtctaattcg ccttgattcc
     4021 attctttaaa gatatcagcc atttcttcta ctgaaaggtt taaaatattc ttcattaagt
     4081 cataagattc agcaatcagt tgcatatctc catactcaat accgttgtgt accattttca
     4141 cataatgtcc ggctccattt gggccaatat atgtgacaca tggtgctccg tcttctgctt
     4201 ttgcagaaat ttgctctaag attggtgcaa ctagcgcata ggcttctttt tgtccaccag
     4261 gcatgattga aggtcctttt aaagctcctt cttcaccacc ggaaacgcct gtaccgataa
     4321 agttgatgcc agagttggct aactcttcat tacgacgcat cgtatcttta aagaatgtat
     4381 ttcctccgtc aatcaaaata tcgcctttat caaggtgagg aagtaactct tggattgttg
     4441 catctgttgc cggtcctgct ttgaccatta gcaagatccg acgtggtttc tcgatagatt
     4501 ctacaaattc ttcgattgta tatgttgctt tgaaattttt atctgggtgt tcttctacta
     4561 cagcggttgt tttagcccct gtacgattga ataaagccac tgaatagccg cggctttcta
     4621 tgttcaaggc tagattttta cccataacgg ccataccgac aacgccgatt tgttgttttg
     4681 acatatgaac tcctcctaca tacgggattt ctccctaatc tcttacatta tagcttaaca
     4741 cggctacatt gcaaactaaa gaacaacata ttt
//
LOCUS       DACDSY010000171         4764 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDSY010000171 DACDSY010000000
VERSION     DACDSY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.174"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(94..1242)
                     /gene="nagA"
                     /locus_tag="IWK44_002612"
     CDS             complement(94..1242)
                     /gene="nagA"
                     /locus_tag="IWK44_002612"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP9551079.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1318..2154)
                     /locus_tag="IWK44_002613"
     CDS             complement(1318..2154)
                     /locus_tag="IWK44_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9551080.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2338..3555)
                     /locus_tag="IWK44_002614"
     CDS             complement(2338..3555)
                     /locus_tag="IWK44_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9551081.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3938..4021
                     /locus_tag="IWK44_002615"
     tRNA            3938..4021
                     /locus_tag="IWK44_002615"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3972..3974,aa:Leu,seq:gag)
     gene            complement(4261..>4764)
                     /locus_tag="IWK44_002616"
     CDS             complement(4261..>4764)
                     /locus_tag="IWK44_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551082.1"
                     /translation="RILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNL
                     FTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRL
                     IMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTF
                     IITVINR"
BASE COUNT         1438 a          997 c          770 g         1559 t
ORIGIN      
        1 tttgaaactt atgtaaagtg atttttacta gaacacaaaa aaaccttaaa accaaactta
       61 agaagtttag ttttaaggtt aacttaaatt atattatgcg tggaaacgtt ccacgccatc
      121 tagataagtt gcttccaatg tcatatcagg gttcaatacg ataaagtctg catcgcgtcc
      181 ttgagcgatt tttccacatg tatcatcgat tttacagctg attgctggaa tcagactagc
      241 catcatgact gcttgttctg gtgttgctaa tccccagtct acaacatttt taattgcttc
      301 tttcaatttc aatatacttc cagctaagtt gccagattct agacgtgctg ttccttcttt
      361 tactactact ggaaattcgc ctaaattgta atttccatca ggcatcccac cggccatcat
      421 acagtctgta attaatgcga catgatcatg tcctgctttt tccattagga ttcccgcagc
      481 atttggatgt acatgatggc catcacaaat caattcagaa aagacatgtt ctaaagataa
      541 taacgcaccc accatacctg gttcgcgatg attcaatcca cgcataccat tgaaagcatg
      601 gacaaataca cttgcgcctg cttcaacagc tttttgcgct tcttctagtg tagcattact
      661 gtgtcctaga gaaacaacta ctccttcttc agtcacttgt ttgacaaact cttctacacc
      721 ttgacgttcc ggtgctaaag caattttttt gattaaacca cctgaagctt cttgccattc
      781 atggaaagta tccaaatcag gatccccgaa ataacttggg ttttgtgccc ctttgtgttc
      841 ttctgtgaag aaaggacctt caaaatagat tcctttgatt ttagctcctg gtgcttcctg
      901 atacattttg ccgattgtct cagctacatc ttttaaacgt tctttgcttg acgtcaatgt
      961 tgttggtaaa aatgaagtca ctccacaaga taacagacct tcagacatca ctttgatgcc
     1021 ttctgcatca ttatccatta catcatgatt catatagccg tgaatatgtg tatctaccaa
     1081 accaggagca atccatttgc cgctttggtc aatgatgttg acttctccct caggtagttc
     1141 tttggtgtat tcaccaaaaa gtccatcggt aatttcaaga tagcctgcat gtttgacacc
     1201 tgacgctaaa aagaatttgt cagcatagat aaaagttctc attctattct ctccctttgt
     1261 ttttctacct ctaaagtact cttatttaga gaagaagtca agaatggtct acaccattta
     1321 caaatttttt gaaatttgtt tcacagcttg tgctaagcct tcatagaatg ctgctctttc
     1381 tggttcttct ttacgcttgt atattaagct tttaagcaca aataaatcat tgaggtaata
     1441 gtagctattc ttctcttgag cccaatagat tccttgattc acctgttcta acgcttcgtc
     1501 gatttcattt tgatggaata agaaatttcc ataattataa aaaatttgcg aaaattcact
     1561 ttttgatcgt tcatcagctg attgatgcag cagttggatg cttcgactca aatagtagcg
     1621 tgcttcagcc gtttttcctg ccacaccata tatttttccg atacaactaa tcagttgtat
     1681 ttctgtgtca gaaacatgga ttctttcttt ttggctagag tagcttaatg cttctttcaa
     1741 ataataaagg gactgagaga tattttgacg caacgtgaat tcgcaaacac cgagataata
     1801 ataatagcgt tgaaagtcag ttgcttgata cagattttca gaaatatcag aagattttag
     1861 tacttgttct aacttccgat aatcacgttt ttggaaacaa ctatccaaca gttcaaataa
     1921 tttatcgttt gtccgagtag tttttcgaga gagattcatc acatgatcaa tcgttacccc
     1981 tagccgttga caaatctgca tcatcaccaa tacattcggc agttcctcat tattttcgat
     2041 ccgacttaat acactttgtg aacaaatatc atgcgaaagc attttttgcg tcattttttg
     2101 ttcttttcga atctctttaa tgatcgttcc aaaggactcg attaacgttt ccataagtat
     2161 cctccccaaa atatgcgtat tcgcattcta ttcgtatttt actttaggaa gtcgcaaact
     2221 tcaagaaaag acttgcaact aaataatata ctttagttat ttaagaaaac tccactagaa
     2281 agagagcctc agtgtgtttg tctgtggccc tcctactgaa aaatttggtt tttaaaatca
     2341 tttttggcga taggttgcta ggaattctct cattttctta gctgtttgtt tcaaatcatc
     2401 cattttagga agataaacga ttcgaaaatg atctggctgg ttccaattaa atccaccacc
     2461 atgtacaagt agaatatgat gttcatgtaa aaagtccaaa acgaattttt catcatccaa
     2521 aatattgaat cgctttgtat caatttttgg gaatatataa aaagcagctt ttggttttac
     2581 ggctgaaaga ccaggaatat cattgattgc attatagata tattctcttt gttcatagat
     2641 ccttccgcct ggcaggagca gctcatcgac actttgataa ccgcctagcg cggtttggat
     2701 aatttgctga gacaatacat tggaacacaa acgcatggag gacaacatat tcagcccttc
     2761 tatatagcct ttgacgtgtg acttatctcc acttaggacc atccagccta ctcgaaatcc
     2821 tgctacacga tgagatttgg atagaccgtt cagcgtaaca acgaacaaat caggcgctaa
     2881 tgtagcaatc ggaatatgtt ctagaccatc catcactaag cgatcataga tttcgtcgga
     2941 ataaataatc aggttatttt gtcgagctac ttcaacgatt tcttctaata attcttttgg
     3001 ataaagtgcc ccagtaggat tattaggatt aatgataaca atagcttttg tccgactcgt
     3061 tatttttgat ttgatatcat caatatcagg ataccattct gcctcttcat cgcaaatata
     3121 atgaacaggt gtgcctccag ctaatgcaat agaagccgtc cacaacggat agtccggcat
     3181 tggaaccagt acttcatctc cattgtccag caacccctgc atacacatcg tgatcaattc
     3241 gctcactccg ttgccggtgt agatatcatt gatcgtcaca ttcggaaaat gtttcagttg
     3301 gcaatattgc tcaattgctt ttctagcaga aaagatcccc ttggaatctg agtagccttc
     3361 tgagtcacgg gcattcatga tcaaatcacg aatgacttcg tttggtgctt caaatccaaa
     3421 aggtgcggga tttcccgtgt tcaattttaa aatactaatg ccctcttctt gcatccgctc
     3481 cgcttcttct agaacgggac cgcgaacatc gtaactgaca ccttctaatt tgttggattt
     3541 ttcaaattct ctcatcactt agccaatccc tcatttcaaa aagttagaac tttccaaatt
     3601 ttttgttatt tactatttca atatcatact ccatcctaga aaattttgcc acttatttag
     3661 gaaaaacgta agtattaaaa aaacacaaac aagcgttgag aacttaattg tcatgatagt
     3721 cattccatag attcgctgcc caatccattg caattccaca aatgattcct agcataaaac
     3781 caagcaaaaa acgtccgaat gcaagtccaa tcaatacacc taagatcatt cccagtcctg
     3841 aaaattctcc gtcatatttc tccatcttaa taagtcctcc catttttgag cttgacaact
     3901 atgtaaaacc tcgctatcat tagtgtgtta ttattacgca gatgtgttgg aattggcaga
     3961 caagcatgat tgagggtcat gtgagcgtta gctcgtgtgg gttcgagtcc catcatctgc
     4021 actacaactc gtgaagttaa ttttgcttca cgagtttttt tgtttgaacg tttctttatg
     4081 ccattatata catatcactg tatcctttac aaaaagaacg tacaatcatg taaacagcct
     4141 gaggtgagta tatgaaggtc ggaaacaagc tactcgtaga aacttgcaag tgttaaaaca
     4201 attaatcgaa aaacgagtaa aatccctcac ctaatactaa agcgagggat tttttgtata
     4261 ttatctgttt atcaccgtta ttataaaggt acaaatacca agcacggtta atccaaattg
     4321 cataaattca tttttttgta cactcatgct tttcattagc tctttaactg ccattagtac
     4381 taagatggcg ataacaacat aaagtatata gtacgtccgc attccggaag cccatactgt
     4441 gggagaaaat cccataatta accttgaaca aaatcccatt atcatcaaaa taactaaaaa
     4501 aatagaagtt ttcttattgt caaaacttaa ataaattcca acaattaaac atagtaataa
     4561 tgccaagact actaaagtag ctatccaagt acctggataa aaaagtgaca gtttcgttcc
     4621 taactctgta aagagattat tcaggttact actactccaa atcattgccc ttttatttcc
     4681 attgacgtaa gtaaagcttt gacccattgt attaccaaaa taaataatca ggtttaagaa
     4741 aaacggtaat gcagtcaata tacg
//
LOCUS       DACDSY010000172         4713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDSY010000172 DACDSY010000000
VERSION     DACDSY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.175"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            108..359
                     /locus_tag="IWK44_002617"
                     /pseudo
     CDS             108..359
                     /locus_tag="IWK44_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            452..1246
                     /locus_tag="IWK44_002618"
                     /note="aph(3')-IIIa"
     CDS             452..1246
                     /locus_tag="IWK44_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9551083.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1656..1767
                     /locus_tag="IWK44_002619"
                     /pseudo
     CDS             1656..1767
                     /locus_tag="IWK44_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1788..1871
                     /locus_tag="IWK44_002620"
     CDS             1788..1871
                     /locus_tag="IWK44_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9551084.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1996..2733
                     /gene="erm(B)"
                     /locus_tag="IWK44_002621"
     CDS             1996..2733
                     /gene="erm(B)"
                     /locus_tag="IWK44_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9551085.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2996..3859)
                     /locus_tag="IWK44_002622"
     CDS             complement(2996..3859)
                     /locus_tag="IWK44_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP9551086.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3861..4133)
                     /locus_tag="IWK44_002623"
     CDS             complement(3861..4133)
                     /locus_tag="IWK44_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9551087.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4150..4365)
                     /locus_tag="IWK44_002624"
     CDS             complement(4150..4365)
                     /locus_tag="IWK44_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP9551088.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4457..>4713)
                     /locus_tag="IWK44_002625"
     CDS             complement(4457..>4713)
                     /locus_tag="IWK44_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAP9551089.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1447 a          796 c          865 g         1605 t
ORIGIN      
        1 atttcttggg agttttgaga ctactatatg cgtttttttg ttgatttacc aacatgttta
       61 gacattttcc tctaggaaaa aattaactag caccacgggt tatcttttat atagaagata
      121 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
      181 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
      241 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
      301 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
      361 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
      421 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
      481 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
      541 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
      601 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
      661 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
      721 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
      781 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
      841 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
      901 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
      961 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     1021 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     1081 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     1141 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     1201 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     1261 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
     1321 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat aggggtcccg
     1381 agcgcctacg aggaatttgt atcgataaga aatagattta aaaatttcgc tgttattttg
     1441 tacatttaac ttgacggtga catctctcta ttgtgagtta ttagtggtac agttttcaac
     1501 cgttttaatt ataaaaaagt ggtgcatttt taaattggca caaacaggta acggttattg
     1561 caggtgtatt tcttatctat gggtttaaca tggattttat cattaaaatc atgagtattg
     1621 tccgagagtg attggtcttg cgtatggtta accctaaagt tatggaaata agacttagaa
     1681 gcaaacttaa gagtgtgttg atagtgcatt atcttaaaat tttgtataat aggaattgaa
     1741 gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg ttggtattcc
     1801 aaatgcgtta tgtagataaa acatctactg ttttgaaaca gactaaaaac agtgattacg
     1861 cagataaata aatacgttag attaattcct accagtgact aatcttatga ctttttaaac
     1921 agataactaa aattacaaac aaatcgttta acttctgtat ttgtttatag atgtaatcac
     1981 ttcaggagtg ataacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa
     2041 aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa
     2101 attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg
     2161 tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaatact
     2221 cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat
     2281 aaaattgttg ggaatattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt
     2341 gaaagccgtg cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg
     2401 gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt
     2461 aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt
     2521 acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca
     2581 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg
     2641 aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt
     2701 aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt gtaaatttgg
     2761 aaagttacac gttactaaag ggaatgtaga taaattatta ggtatagggc acctctaata
     2821 actaccaatt aattcacctc aaaatgaaat tagaaaaaaa cagcgaaatt tttaaatcta
     2881 tttcttatcg atacaaattc ctcgtaggcg ctcgggaccc caacatgagg aaatgttttt
     2941 ataaaaaaag agtagttacc gttttttggt aactactctt ttcctttttt aaggtttaaa
     3001 tacctggaag tttaggtgtt ttgggtattg gtggtgtagg tggctgcaag ctttccaatt
     3061 tttgttgaat tgctttaaat tcaggtgtct cttggtgttt atttagaacc attttttgct
     3121 ctattcgttc taaagtcggt tgaatttctt tccctgatac tttacgattc aattcttttt
     3181 ctaaggtttc ttttggacta atagaaggtg tttccaaact tgaatagagt tttactcctt
     3241 ctctgttata tagccttata tcgctaaata agcccgtttt atgaagggtt tctaaattgg
     3301 tcggtaagtt tttgacaaca atatcatgcg cttgttttgg tgttgccctg gctgtcattg
     3361 gatcatctgc atacatggtt tcatatcgtt caattgttcc taaatatgag ttgattttag
     3421 gtactgccat gacatacatt tttgtttcat aacctttggc ttgaagcatt gttgcggttt
     3481 gaataggaac gtctgttgtt cgtcctgtac cttcgatcac caaattatac ccttgatcgc
     3541 tcaaacggct tatgatcgct tctgtcatgc gattagaata aggggtaacg tgttttacta
     3601 cgtctttttc ataaagtttc actagttcat caaaattagg gtgctgttgt ttaaaggtat
     3661 cattatcaat gacaataaca ttcccttgtg tttcttcaaa aattgctgat cgcaaactgg
     3721 ttttccctga ccctggttgc ccaccaagta aaaaagcggt tggcgattca accgcttttt
     3781 ttccttgaat caattcttct aaattatcat ttaagcgatt ctcaaattgt ttgtcagtaa
     3841 aattgactat atttgccata ttaagccact ttctctttat tcaaaacttg ttttgagtaa
     3901 cgattcattg accgccaata ctcatgaacg gcttgtaatt cttctaaaga ataatcaaaa
     3961 agatttacac gttttgcaag ctttaattgg tttaataaat tgacttccaa taattgagag
     4021 tcatttttat ttaattcatg gttcaaaaca tagtttaata ctcgattata aacgcttgcc
     4081 gataattcgt taatgatctc tatttcaaat gttttttcat acgtaactgc catttcattt
     4141 tcactccttt taaagtttgt caggtaaata tttccgtata tattcttcta aggcttgatc
     4201 cataacttct tttacgtttc cgccattctt tgctgtttcg atttttatga tatggtgcaa
     4261 gtcagcacga acacgaaccg tcttatctcc cattatatct ttttttgcac tgattggtgt
     4321 atcatttcgt tttgcttttt gtgcgcctaa atttcccaca atcactcact tctttctatt
     4381 tcttcttact cttattttat catcaacaat cacaaatcac aagtgatttg tgattaatca
     4441 cttgtgattt gtgattctat tctttttcgt tttctaattg aataattcgc tcaagcattt
     4501 caaaaaatac gttcttatac atggataaaa cttttttgtc atagccttta tgctctgtaa
     4561 tgccgttttt agaccaggta cttactttat tacttcgctt gataatattt tggaaaacca
     4621 agttatcttc tttatgttgc ttatacagtt cttccaggtt tgattttatc gttgcgctgt
     4681 ccgtatcaac taaataagga acaaaaccga tca
//
LOCUS       DACDSY010000173         4710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDSY010000173 DACDSY010000000
VERSION     DACDSY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.176"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            55..1683
                     /gene="treC"
                     /locus_tag="IWK44_002626"
     CDS             55..1683
                     /gene="treC"
                     /locus_tag="IWK44_002626"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP9551090.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(1743..2924)
                     /locus_tag="IWK44_002627"
     CDS             complement(1743..2924)
                     /locus_tag="IWK44_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9551091.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            3176..4699
                     /locus_tag="IWK44_002628"
     CDS             3176..4699
                     /locus_tag="IWK44_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9551092.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
BASE COUNT         1723 a          822 c          948 g         1217 t
ORIGIN      
        1 ttgtatatat aagctgtggt aggtaataaa tcagttgaat ggaggtaaat atccatgaca
       61 tttaaagaca aagtcattta ccaaatctat cccaaatctt acaaagacag taatggtgat
      121 ggaattggag atttaaaagg aatcaaagaa aaactcccgt atctgaatga tctaggcatc
      181 gacatgattt ggctaaaccc tatttatcca agccctcaaa aagacaacgg ttatgatatc
      241 tctgattaca cagccatcga tcctatcttt ggtacgatgg aggaattcga acagctaata
      301 gccgaagcaa agaaataccg gatagaaatc atgctcgata tggtgctgaa tcacgtttcg
      361 acagaacatg aatggttcaa aaaagcacta gcaggggacc agcattatca ggacttcttc
      421 attttaagag aagagccaac ggattgggta tcaaaatttg gtggaagtgc ttggtcgcca
      481 tttggagata cggggaaata ttatttgcac ttatatgatc ggacacaagc tgacttgaac
      541 tggcggaacc aagcagtaag agaggaacta ttcaaagttg ttcgtttttg gatggaaaaa
      601 ggggtaaaag gattccgttt cgatgttatc aatgttatcg gtaaagacga acaattgaaa
      661 aataataaag aaaatgatgg caaacctgaa tatacggaca aaccaatcac ccatacgtat
      721 ctaaaagaat tgaaccaagc aacgtttggg acggattcca ctatcatcac agtcggtgaa
      781 atgagctcta cgacgatcga aaattgtatc ttgtatacag aacctagtag agaagaatta
      841 tcaatggtct ttaatttcca tcatttgaaa gtcgattatg aaaatgggaa taaatggtct
      901 actcctccat ttgattttgc agaattaaag cgactatttc atacatgggg agaaaatatg
      961 agtgagggta acggctggaa tgctctattt tttaataatc atgatcagcc acgtgcgctc
     1021 aatcgatttg tagttatcga tcagtatcga aaacaaggag cagagatgct agcaaccatg
     1081 atccatttga accgtgggac gccctatatt tatatgggag aagaaatcgg catgatcgac
     1141 ccagactatt catcgatttc cgactatatt gacgtagaaa gtttgaatgc ttataagtta
     1201 ctccttgaac aaggattcac agaaaaagaa gcgtttcgtc ggatcaaagc aaaatctcgc
     1261 gataactctc gaacacccat gcaatggtca gacacagaac aagcaggatt tactacaggg
     1321 aaaccttggt taaaagttgg aggcaagcta gaagagatta atgtagaaaa agaactatct
     1381 tctgaagatt ctattttgtc ctattacaag aaattgattc ggctgagaaa aacatatcca
     1441 atcgttgcac aaggagatta tcatgcttat gaagcaaatc atccggaagt ttatggcttt
     1501 cttcgccaat ttgaaggaca gcagctgctt gtattaacaa atttctatga taaagataca
     1561 aggatcgcca tcccagaagg attcacagaa gccaatattc ttatcgataa ttattccgat
     1621 actgttcttc atcaaagtct ggagctaaaa ccataccagg caattgcttt gctgaaaaat
     1681 taaaacaaaa acacaaaacg tcgttcctac gagctattct cgtaagatcg acgttttgtg
     1741 ttttatttaa aataatcttc ttcagaaaat gaagagtttt catactctcc tttatcgcca
     1801 taaaatttat tgtagcgttg tttgaattta cggaaaataa aagtaacgag taatttgact
     1861 aacgtataga taggaatacc aaaaataacg cccacaattc ccattagatc acccatgaca
     1921 agtaaaacga ttaagatcgt aatcggatgg atctgtaaac gatcacccat tactcgagga
     1981 atcaccaagt caccgtggag aagttgtacg gcaaaccaga caatgacaaa tttgacagcc
     2041 ataaaagtag agtcttggaa tgcaatgatc aaacctggaa agaaggcgat aaacggccca
     2101 atatatggaa tgatacataa caacccagca gaaatagcta aaacactagc aaattctaat
     2161 ccgatcagta agaaacaaac ccagtagact gctccaagga taaatgaagc aatgatttgc
     2221 ccctttagaa atgaacccaa ttgttgatta gcaatctttg tcaaattttt aaaatctgta
     2281 cgaaatgctg gtgggacaat cgctaaaact gactgataaa atttttgagg atctctcaaa
     2341 aggaaaaaag cgacaattgg accagtaaat aaagtaataa aaatagctgt aacagttgaa
     2401 aaaatgttgc tgatccgttg tgcgccactt tcccaataat ttccaataaa tccagctata
     2461 tcagacgtca cgtcgtcaat agaagcaaaa aattgattaa atgaatcggc aaaaggtgtt
     2521 ttctccaaaa attgtcggag actctcttga aaatcttgaa acaaatctgg caaactactg
     2581 atcaatgtct ttgcctgttc ttcaagaaaa ggaataagcc atatgcttcc aagccccaat
     2641 aaaaataaaa taatcacata aacgataatg actgacaagt tcctcgaaag gtgcttgctc
     2701 aaacgtttga tgactggttc caaaagataa tacaagatca gtgcaaacaa taatgggggc
     2761 aagatagtag cgataatgat tccgacaggc ttgaatataa acgagatctg atggaaaaaa
     2821 taaatgatca gcgcaagtaa gataactatt cctaaaacat agtaagaggt ttttcctcct
     2881 aaaaagcgga tcaaacgatt cgttgtatct gtttcttttt tcactctttt tcctccttta
     2941 tctataactt cattatagac aaaagcgggt ataaaaggaa aatgatagag gttgattgca
     3001 aaaatatcgt caatcattca tataaacttt ctcatcaatt aaaataagaa aacgcttgac
     3061 agttctttta accatatgat aaattaaatc ctgttgctaa aacgtaacta ttacggtttg
     3121 cgaaaagcga atcggaacat tcaaatgaaa gaagatgaga agaggagtgt cataaatgaa
     3181 acgattgata ggtatattgg ccatgctggt tttagcaggt atgttaactg cttgcggggc
     3241 aagtgggaaa gccgaggaca gtaaagaaaa attgagcgtg atgacgacct tttatcccat
     3301 gtatgatttt acaaaagcaa tcgtcgggga tgaaggagaa gtagagttat tgattccagc
     3361 agggactgat tcccacgatt atgaaccttc tgctaaagac atggcaaaga tccaagatac
     3421 agatatcttt gtttataatg atgaaaacat ggaaacatgg gtccccgcaa tccaaaaaac
     3481 attacaagaa ggcaatgtcc atacgataaa agcgacagaa ggtatgcttt tacttccggg
     3541 aagtgaagaa ggacatgatc atgaccatga acacggggaa gaaggccata cacatgagct
     3601 agacccacat gtatggctag ctccaagttt agcaatcaag caggtagcaa acatacgtga
     3661 tcagctgatt gaagcttacc cagaaaaaca ggaagtatgg acaaaaaatg cagcagccta
     3721 taccgagaaa cttcaagcac tccatcagct ttatcaagaa acctttaagc aagcaaaaca
     3781 aagatctttt gtcacgcaac atgctgcatt caactatcta gcgttagaat atggattgaa
     3841 tcaagtatcg attgctggtc tttcttctag tgaagaaccg tctgcggcga gaattgctga
     3901 actgaaacac tttgttaaag aacatggaat caactatatt tattttgaag aaaacgccaa
     3961 agatagtatt gccagaacgt tagcaaacga agcaggtgta tctttagaag tattgaatcc
     4021 tttggaagga ttgacgaatg aacaaataga aaatggagaa aactatcttt ctatcatgga
     4081 agcaaacctc gaagcgttga aaaaaacgac agagacagaa aacccgctag aagatacttt
     4141 gacccccaaa aaagaaaaga gcgtctacaa cggttatttt gaagacgagg atgtacaaga
     4201 tcgctctctt tcagattggg caggaacttg gcaaacagta gatacgtatg tagaagatgg
     4261 gacttttgat cctgtttttg aatataaaga aaaattgaat caggataaga cagcacaaga
     4321 atataaagaa tattatacga agggctatca aacagatatt gcttcaatca agatcagtga
     4381 gaaagagatg gcatttacgt ttaaggatgg aacaacgaaa cgttcaaatt atcgttatgt
     4441 agggaaagaa atcttgacct acgaggcagg aaaccgtggc gtaaggtatc tattcgaagc
     4501 agaagataag gaaagtggcg cttttagata tattcaattc agtgatcaca cgattgcacc
     4561 ggctgactcc gaacattttc atttatatgc aggaaatgaa agtcaagaag tattgctgaa
     4621 agaaatggag aattggccaa cattttatcc agatacaatg gatggacgtg aaattgcaca
     4681 ggaaatgctt ttgcactgat aggcaaaaaa
//
LOCUS       DACDSY010000174         4635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDSY010000174 DACDSY010000000
VERSION     DACDSY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.177"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            175..519
                     /locus_tag="IWK44_002629"
     CDS             175..519
                     /locus_tag="IWK44_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP9551093.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="IWK44_002630"
     CDS             520..939
                     /locus_tag="IWK44_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP9551094.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="IWK44_002631"
     CDS             complement(1031..1381)
                     /locus_tag="IWK44_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551095.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2567)
                     /locus_tag="IWK44_002632"
     CDS             complement(1347..2567)
                     /locus_tag="IWK44_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP9551096.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2933..3499
                     /locus_tag="IWK44_002633"
     CDS             2933..3499
                     /locus_tag="IWK44_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9551097.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="IWK44_002634"
     tRNA            3644..3731
                     /locus_tag="IWK44_002634"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            4065..>4635
                     /locus_tag="IWK44_002635"
     CDS             4065..>4635
                     /locus_tag="IWK44_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP9551098.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1685 a          778 c          891 g         1281 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata
     3781 agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca
     3841 gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac
     3901 agccccttat tgttgtactt ttaacctaca atattttttg attccccctg agtactattt
     3961 tttataaatg ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa aatactagat
     4021 gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca
     4081 cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg
     4141 aagtggtcac tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag
     4201 ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg
     4261 aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt
     4321 ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac
     4381 tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca
     4441 atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag
     4501 aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg
     4561 gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct
     4621 cgctatcaaa tggaa
//
LOCUS       DACDSY010000175         4610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDSY010000175 DACDSY010000000
VERSION     DACDSY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.178"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(85..339)
                     /locus_tag="IWK44_002636"
     CDS             complement(85..339)
                     /locus_tag="IWK44_002636"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9551099.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(450..704)
                     /locus_tag="IWK44_002637"
     CDS             complement(450..704)
                     /locus_tag="IWK44_002637"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9551100.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(897..1172)
                     /locus_tag="IWK44_002638"
     CDS             complement(897..1172)
                     /locus_tag="IWK44_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP9551101.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1144..2097)
                     /locus_tag="IWK44_002639"
     CDS             complement(1144..2097)
                     /locus_tag="IWK44_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9551102.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2709..4202
                     /locus_tag="IWK44_002640"
     CDS             2709..4202
                     /locus_tag="IWK44_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP9551103.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4337..>4610
                     /locus_tag="IWK44_002641"
     CDS             4337..>4610
                     /locus_tag="IWK44_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9551104.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1542 t
ORIGIN      
        1 tgcttgaatt cttgtgaaaa agttcttcgt gttctcttag acataaaaat tcctcctggt
       61 atgttttctt ctagtctaca caccttaatt tttctgtcta gttaattgta gcctatccat
      121 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
      181 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      241 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      301 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      361 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      421 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      481 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      541 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      601 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      661 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      721 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      781 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      841 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      901 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      961 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     1021 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     1081 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1141 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1201 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1261 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1321 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1381 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1441 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1501 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1561 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1621 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1681 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1741 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1801 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1861 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1921 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1981 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     2041 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     2101 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     2161 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     2221 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     2281 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     2341 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     2401 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     2461 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     2521 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     2581 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     2641 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     2701 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     2761 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     2821 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     2881 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     2941 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     3001 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     3061 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     3121 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     3181 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     3241 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     3301 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     3361 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     3421 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     3481 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     3541 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     3601 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     3661 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     3721 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     3781 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     3841 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     3901 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     3961 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     4021 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     4081 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     4141 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     4201 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     4261 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     4321 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     4381 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
     4441 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
     4501 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
     4561 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa
//
LOCUS       DACDSY010000176         4543 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDSY010000176 DACDSY010000000
VERSION     DACDSY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.179"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(92..703)
                     /locus_tag="IWK44_002642"
     CDS             complement(92..703)
                     /locus_tag="IWK44_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9551105.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(826..951)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1033..1749)
                     /locus_tag="IWK44_002643"
     CDS             complement(1033..1749)
                     /locus_tag="IWK44_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9551106.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1755..2324)
                     /gene="scpB"
                     /locus_tag="IWK44_002644"
     CDS             complement(1755..2324)
                     /gene="scpB"
                     /locus_tag="IWK44_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP9551107.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2308..3105)
                     /locus_tag="IWK44_002645"
     CDS             complement(2308..3105)
                     /locus_tag="IWK44_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP9551108.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3075..3446)
                     /locus_tag="IWK44_002646"
     CDS             complement(3075..3446)
                     /locus_tag="IWK44_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9551109.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3465..4352)
                     /gene="xerD"
                     /locus_tag="IWK44_002647"
     CDS             complement(3465..4352)
                     /gene="xerD"
                     /locus_tag="IWK44_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP9551110.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         1241 a          988 c          790 g         1524 t
ORIGIN      
        1 caaaacaaac aaataaaacg tttacattat tgcatgtctt gtattttttt ccaaaaaaaa
       61 agaccaagaa atttcttgat ctttttgata tttatttgac tgctgtatga cgttgttcga
      121 actgatgttg ttttcgactt aaccatggaa gaagttgcgc atgcagcact aaaaagacag
      181 cactcacgat gaatcctttg atcaagttga aaggaacgat cccaatcagt acatagtttg
      241 ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa aaacattaag taaagaggcg
      301 taataacgaa ataatttgcg atactcataa aaatagttag agcgatggtt cctgtaatga
      361 cacctaaaaa tttgtttctt cctttatgct gtttctttac actaaagaaa taataaatcg
      421 ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa ttatccggag
      481 aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa cgctgttgca actccggccg
      541 ctggaccgaa taagaacatg ctgatcaata ccgagatatc actaaaatcg atcttcaaaa
      601 aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg atcgcagcaa
      661 acatcgctac tgcaaccatt ttctgtacac gggtgttctt catttgaaat cctcctgtta
      721 ggactcctct tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact ctattttcca
      781 gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa ataagctcta tcttctttat
      841 ccagactata ctgtcggtat cggaatttca ccgattcagc tatttttttg caaaataggt
      901 cgtggactat aaccaccggt cgggaatttc accctgcccc tgaagacgaa ccttatataa
      961 tttttgttta catattcatt atacatatct attcaaaaaa cgcaacatct aacaattata
     1021 aaaagtaagt gttcattttg agtggttcag caaactgctg acttcttttt tgttcagttc
     1081 acggtaatcc ccaggacgta ggccttgtaa agtcagatct ccgtattttt cacgttttag
     1141 tttttgcaca ggaagaccaa ctgcttgaag catatttttc acttgatgat tacgtccttc
     1201 gtggatcgtc aattccacga tactgtggtt tgtcttagga tccactgata aaatttggaa
     1261 tttagctgga gatgtttttc gtccttcgat ctttattcct tttgtcaaag gtgcaagcat
     1321 cgattttgta gcgatccctt ttacctttgc aacgtaaact ttatccactt catgttttgg
     1381 atgtgtcagg cgctgagcaa aatctccatc atttgtcagt agcaagatcc ccgaagtatc
     1441 gtaatccagt cgacctacag gatagatacg ttcttttacc atcggcaaaa gatccgtaac
     1501 aacttttctt cctttgtcat ctgcaacagc agaaataact cctttaggtt tgtacaataa
     1561 atagtaaccg tattcttctt gataaattgg gacttcatcc acttccacag tgtcatgttt
     1621 cccaactttt atccctaatt cccttacaac ttgtccgttt acttttacac gtcctgcaag
     1681 gatgaattct tctgcttttc gacgagaagt gatgccagcg tgggcaatta ctttctgcaa
     1741 tctctccatt acaattattc tcctttattc attccatcaa agaatagatc tggtgacacg
     1801 tcttcttcaa gtgaaatttc catttcttgt gtatcaggca gttcttctaa tgatttcaaa
     1861 ccaaaataat ctaagaagta gccagttgtt ccatataata tcggccgacc tggtccttct
     1921 actctacctt tttcttcaat cagtttatgg gcagctagac gctgtaccgc tcctgaaacc
     1981 tgaactcctc gaatctgctc gatttcagct cttgtgattg gctgtttata agcgatgatt
     2041 gctaaggttt cgacggcggc ttgagataat gtgtttgatg tcccttgcgc atatcgctta
     2101 agtaagggag ccagctcttt tttagtcgtt aatacataat ggctttccgt ttcaaaaata
     2161 tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta attctttcac taaacttgtg
     2221 acttctgctt cgctgagatt taacagtccg cttaattctt cattactgat cccttcctcg
     2281 ccaacaataa aaagaagggc ttcaagttca cttaatttcg tcatgttcgg tatctcctac
     2341 tgctggataa agcataattg attcatagtt ttctttttgt tgcgcaatga tctgcccttt
     2401 tttcatcaat tctagcaaag ccataaaagt agtgacaact tcacttttag aaggtgactc
     2461 aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt atacgctctt cgataaatac
     2521 cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt tttgagaaaa
     2581 tttcttacgt tctaagattt tctgcatggc acggatcaaa tcgatttttt tgacttgtcc
     2641 tcgcttcaac ggcttttcag catccgctaa cccatcgatg ttcataggtt cttttgtgta
     2701 ataaagactt cgttcctctg ctttttctgt cagtctttca gctgcatatt tatatttacg
     2761 gtattctaaa agctgagtga ccaattcttc tcgaggatct tcttcccata attcttcatc
     2821 gtctgtgccg aattcttgga caggaagcaa tgtcttgctt ttgattgcca tcaatgtagc
     2881 agccatcact aagtattctc ctgcaacagc tagctcgagt gttttcattg tatggatata
     2941 attcatatat tgctccgtga tcgtagcgat cggtatatca tatatatcga tttccatttg
     3001 ctgaatcaga tgaagcagca gatcgagagg accctcgaat acatctaatt taatattgat
     3061 ctccgtcaac tcattcactc tttcttcttt ttctttgcta gccattttcc aatgtaaggc
     3121 tctgtgattt cagttgcacg gatggagaca tccgggtact tttcctgtaa ttcatctaac
     3181 atttgaaagc ccatattctc accgcgataa gacggattca aagaaatgtc atggatgatc
     3241 agctgatcgt tcgttgccca gtaaatacca ataatccctt gaatgttggt tgaaccttct
     3301 ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt acgtatcaat ctccttcaac
     3361 aaagaagttt tacctgtcag tttcttatgg aaactcaata gtcccatagc aattttacgg
     3421 ttttctttcc ggtaatgtgt taacatcttc tcaccttcct atgactaagc ccttggaaaa
     3481 tgctgcttat aaacatccgt cattcttttc ttcgtgatat gtgtataaat ctgagtcgta
     3541 gagatatccg cgtgacctaa caattcctgt actgtccgca aatcagctcc attttctagc
     3601 agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta cttctttgta aatcccagcc
     3661 tctcgtacta actgcttcag atttttccag attccttgcc gagaaagacc tttcccatga
     3721 ttattgacaa atacatgcat ttcagatgcg tcctttacaa gtaatggacg tgcttcgtct
     3781 aagtagcgtt ccagccattg gatcgcataa tcacctaaag gaatgatccg ctctttatct
     3841 ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa tttcaatcca
     3901 atcaattcac tgacgcgcat cccagtagca tacatgactt ccaagattgc acgatctctt
     3961 atccctaacg tttttgtcgt atcaggtgct tcaatcagct tttctacttc tgtcagagtc
     4021 aatgtacttg gtaatttctg tgctttcttc ggcgtatcga tatgttgcat cggatcatga
     4081 tcagtgatac gctcttgccg taaaaactga tggaatcttc tgagacttga gatcatccga
     4141 gtaacagttg ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg gatgactaaa
     4201 aagcgatcga catcctgcca tgaagcaatt ttctgctcct gtagaaaatg caagtactgt
     4261 tccaaatcac gttcatagct ttgtcgtgta ttggcggaca atccccgttc aatattcaag
     4321 tagtggagat aatcgttgat ttgctctttc ataataatcc ccctcatgcc tctctatcat
     4381 atcagatacc gcttcattct gatagtcttt ttccataatc tgagcaaaga aaaagccgtc
     4441 tagacactca gacggactgg tttctttttt cggctctata atttatggga ctgatgaatc
     4501 agaattgatt cttcagcccc attttcattt taggtattaa aaa
//
LOCUS       DACDSY010000177         4474 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDSY010000177 DACDSY010000000
VERSION     DACDSY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.180"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            322..1053
                     /locus_tag="IWK44_002648"
     CDS             322..1053
                     /locus_tag="IWK44_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9551111.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1198..3225
                     /locus_tag="IWK44_002649"
     CDS             1198..3225
                     /locus_tag="IWK44_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP9551112.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3333..4430
                     /locus_tag="IWK44_002650"
     CDS             3333..4430
                     /locus_tag="IWK44_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9551113.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1573 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaaatataa tatacatttt ttatttaatt ataattaata ttgattgaag tctaggcagt
       61 caagtcacat tttgatagat aggaggggga cttacgaatt attttaaagg ctatgacagc
      121 tgtatttcta gattgtgttt gcatcaagtt gcaataaaaa ctagaaatca aatcgggctg
      181 ataatcattt ataactaatc gatacatagg accaaatata ggtctatgtt cttttatttc
      241 ataagtatcg taaaacagaa ttttgctatg gattaatcca atcgactgat atgtttagta
      301 aataaagaaa gaggaatgtc aatgatgaaa aaagaaatcg tattaacagc agcaatgatg
      361 ttattcagca tggtagcaag tacgactttt gtttctgcaa ctgaagtata cccaaaggaa
      421 tataacacag aaggaacgat tacatttgaa gcaggagatg agggtgtaac tcctcctgtt
      481 gatccagaga atcctgatcc caataaacct gtcgatccat cagatcctcc aagccctgga
      541 acaggagggg cattatcgat tgattatgga tcaaaattca aatttgggac tcaaaaaatt
      601 tctactgcgg ataaaacgta ttatgcagct gctgatgtga tgaacgatgg gtctagaaaa
      661 ccgacatatg tccaagtgac agataggaga agtaccttat ctgggtggaa attaagtgtc
      721 tctcaaccag aacagtttaa aactgccagt ggggatgaac tagttggagc acagctgaaa
      781 tttacgaaag ggcaggctgt ttctttggtt gatccaacat atacgccgca aacggtgaat
      841 tctgaattaa cgttgacacc gggaggaaac aatacgttag caattaatgc taaatcagga
      901 acaggtgtgg ggacttgggt ctatcgattt ggtgcaaatg aaaatgaaaa tcaagatgcg
      961 gtacagcttt cggtacccgg taaatctgtc aagttagcgc aacaatattc cactaaatta
     1021 gtttggacat tagaagatac acctaacaac tagtttactg aaaagaactg ttaaaaataa
     1081 atacgtagtc atattgtctt gcgaggtaat cgagcaaaaa ttgtcaaagg ctaggctaaa
     1141 tttgtttgga tacgaatagt tcatctagga atttaacgat caggaggaaa agatatattg
     1201 aaaaagagga ggatcgtgct cattttaagc agtctcttag tgactaatat ctttccgatc
     1261 atagggtact ctttggaatt gaatcagttg gagcatggaa atagaagaga cgaagaaagc
     1321 aaactcactt cgacattcga cagtttaata gaatccaacc agacacaaga aagcagtgag
     1381 gatttaagta tttcgagtag tggagagacc actgacaacg tagaagctga gggttcggac
     1441 aaacaaggac tagaagatgc accaatttct gcggttgagc tgcctgacga tcccaatatt
     1501 atttctatcg ataaaatttt tcaaacacct atcggtacta gcacgtccat attggaaaat
     1561 gggaagttac tgcagttgaa cccagcagaa aggtctcaaa gaggagctat ttggtcaaat
     1621 aagaagatta gtttactctc ggattttaca tttaaatctt atttatactt aggaaatcaa
     1681 cgagatagag cgggagatgg catgaccttt acattgactg gcgatcctag aatggctagt
     1741 aatcccagtc aagttattgg atcgccaggt atgggaattg gctcatattc tactcgttct
     1801 ggccaacctt acattgcgaa tgcgctctcg attgagtttg atacgtataa aaatagtggt
     1861 tcttctgatc gaatggatcg agagataact gctgataata gagggcatgg acatgtcgct
     1921 tttgtcacgc ctaaaccaaa taataataat tattctggag agcatactgg cgtgacggta
     1981 gctcctacat ttctttctaa tggcacatgg cgcttgttaa taattaaatg ggacgcggag
     2041 caaagaagtc ttacttatga tttggaaggt gtcggtcact cttctcggat tatacctgat
     2101 ctcattaggc agtttggttc tacagaagta aattgggggt ttacttcttc aacaggtact
     2161 ttttttcaag aaaatgctgt cgctgtcacg cgacttccat cgaatgtcac atcaacagct
     2221 gatgtaaaag tcaacgatag tgattatggg gccatcgctg aagctggagg aaacgataag
     2281 ataactatca gaaatacatt gaaggtcgag ggggattttc ttgaatataa gaaaatgcag
     2341 actagtatcg acctcccaca agaattaact attcccgagc atatcttgat caatggaaat
     2401 caaattgatg ccaaagatat cttgataacc ggaaatcgag tcacgctaaa tctgacagat
     2461 tattttattg ctggagatac gttaataatc gaattggaga caactttgca ggaagtaatt
     2521 cctgaaaaag tactcactat gcattttgaa taccttgaaa acgggaattt gattgagcaa
     2581 tcgaatgaaa taaacctaaa gatcgcaaag ctgaaggaga tagatatcca tgtttattat
     2641 aaagataaag acagtcagat tgatattgct gaaatgaaaa caatcactgg gaaaatagga
     2701 gagaagtata aagaaactcc tattgatatt gaagggtatg tttttgactc ggattcagcg
     2761 aatacagaag gcatcttctc cgaaaataca gaggatatct atttttatta cagaaaaggc
     2821 aaactttatc tctcaactgc tcctcaatat gtcaattttg gaaaacataa aatatccagt
     2881 agaccattaa gtgaattcgg acagttttca ggtgggttga aaattataga tgaacgggct
     2941 tttggaagtt ggaaattaca attgaaacaa gagaaagttc ttacaaatgg agattttgag
     3001 cttccagaag ctctttcctt tgttacctct gaaaatacaa cggttatagg gacatccgct
     3061 gttacgattt tcgaaagtga tcaaaagggt gaaagcgatt tgtcctctct tttagattct
     3121 acagctcatc gtggaatcag aatagatatt cctgtagaaa accaaagaat cggaaccttt
     3181 gaggggaaac tatcttggat acttgcagat gtcccaggaa attaacagga agagaagaat
     3241 ataacaaaat tattgagttt caagaagggc acaggtacca taggctcttg tgtcccacag
     3301 agacaagatt ttgttatagt cttctcttac atatgattaa aggagcgatt ttcttgagac
     3361 caaaacaaca attatttata acactcctag ttattttagg ggcacttttt aacaatatat
     3421 cagtgtcagc tagtgcattt aatttttcag tgacgccgat aacttcagag aatcaaatcg
     3481 acaagagaaa aacttacttc gatcttcaac ttgatccgga tcaggaagta gaagtaaaag
     3541 cggaattgag aaatgataca gaaaaagaag tgaaaatcga tatttctgtg aatagcgcaa
     3601 ccaccaattc aaatgtgatg gttgaatacg gaaaaaacga gattgaaaaa gacatgagcc
     3661 tgatctttga tctggtagat tatgtgagct atccacagat agtgacgcta aaacctaaaa
     3721 gtgtccaaac cgttgctttt catgtcagga tgccgaatga gcgatttgat ggtgtattgg
     3781 ctggtgggat cacattccaa gaaatccaga cggaaaagga ccaaacagaa actaaggatc
     3841 aaagcttatc gattgagaat gaatacacct atattattgc tttgttgatg caacagacac
     3901 tgaacgaagt tgctccgaac cttactcttc atgaagttaa accagatcaa atcaatgcaa
     3961 gaaatgtcat cttagcaaac gttcaaaatg atcaaaaaac ctatatcaat caagtagtta
     4021 ttgaaacaaa aattacaaaa aaaggacatt ctgaagtttt atatcaagag gaaaaagaag
     4081 gattgcagat tgcgccgaat acaaattttt cttttccaac tgctttaaat ggtcaaccgc
     4141 taaccccagg tgaatatcac cttacaatga ctgttttagg aaatgaaaat gaaaatggaa
     4201 aattttcgag aaaaaaagga aatactacga tcaactatac caatcaatgg gtatttgaaa
     4261 aagattttac aatagacgga aaagtagcca aggaattgaa cacgacggat gttacgatta
     4321 aaaaagacaa taattggatt tatatcttag tagcttcact tttcttattg tttattttat
     4381 tatgcatact tttgtttgtt ttgagaaaaa aagaagagaa agaaaagtaa ggttaagata
     4441 acaaatagta aatttttatt attgtaggat ggtt
//
LOCUS       DACDSY010000178         4372 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDSY010000178 DACDSY010000000
VERSION     DACDSY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.181"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            380..1291
                     /locus_tag="IWK44_002651"
     CDS             380..1291
                     /locus_tag="IWK44_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551114.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1778..2044
                     /locus_tag="IWK44_002652"
     CDS             1778..2044
                     /locus_tag="IWK44_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551115.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2116..2457
                     /locus_tag="IWK44_002653"
     CDS             2116..2457
                     /locus_tag="IWK44_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551116.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2512..2931
                     /gene="ssb"
                     /locus_tag="IWK44_002654"
     CDS             2512..2931
                     /gene="ssb"
                     /locus_tag="IWK44_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9551117.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3008..3304
                     /locus_tag="IWK44_002655"
     CDS             3008..3304
                     /locus_tag="IWK44_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551118.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3319..3750
                     /locus_tag="IWK44_002656"
     CDS             3319..3750
                     /locus_tag="IWK44_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551119.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            3932..4237
                     /locus_tag="IWK44_002657"
     CDS             3932..4237
                     /locus_tag="IWK44_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551120.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         1605 a          671 c          934 g         1162 t
ORIGIN      
        1 cccctaaccc cctctttcag cctcttttga aagaggggga gaatcaaccc cacggccaac
       61 gccccagtgt tcttggggct gtccagaaca gaaacttgcg agagagtggg ggtaaaacaa
      121 gtgtagagga aacttgaaga cttgttcgaa cagaaacttg cgagagagcc ggagtaaaac
      181 aagtgtagag gaaacttgaa gacttgttcg aacagaaact tgcgagagag ttggagtaaa
      241 acaagtgtag aggaaacttg aagacttgtt cgaacacaaa cttacaagag aggggagtaa
      301 agcagttgca gaggaatcag gaagatttgt tcgaacagaa acttgcaaga aagcattatt
      361 aaattgaagg tgatgaaata tgaagtattt attgcatggc tattttattg attattctac
      421 tcgcaatagt agtactttag cgattctatg tttgcttcat gaattgagag tggttactag
      481 aaaacagcta tacaaactat tgaattttga cagatcaatt agtaaacaag gagtaacgaa
      541 tgctttagtg agattatatg aaaaacagtt aatcgatcga aaaaaatatg gaacagaaat
      601 gtattattat ttgacgaaag agggacatca aagcataggt gggtattata cgttgccgaa
      661 agttcctgaa tataatttga atcaccattt acaaattaac aattatttga ttaaaggaat
      721 ggcattggca aaagaccacc cacatttaaa acggattatt tcagaaagac gtaccgtcta
      781 tgaaacgaag gatttaaatc ctaagacaaa aggaagaaaa ttttttgtgc cagattttat
      841 ttttcgtttt cgagacaaag aaaatcatga gatcaattgg tcctttgaaa ttgagctaac
      901 gttgaaaaca cgtcgtcgct accgtgaagc gatttttccg aaatatattc gtgagttgcg
      961 aacacgtcct gaatcacatt taatttatgt gacacctagt ggcgcaattc aagaagaact
     1021 tcatgcgttt aagcgtattt ttttcaagcg cgaaactgaa aaagatgcga gtcaacaaga
     1081 attgtttgag cggttgcata ttttttcagc tttagagttt gaagagggat taaaagcatt
     1141 attagagaat gatccagcag tcaattggcg gacagtagaa cgagaaaaaa tttatccgaa
     1201 tggcgctgtt caaagtcgtt tgaatttatt tgaagaatca actgaaaatg aggaaaaacc
     1261 aaaagtgaag tttacgcctg aagtattgta aaagagggtg ttcgcttcgc tctttttttg
     1321 tgccagtttg ctggcaccgt tcagacgcaa gcgtctgaac cggctcgttt ttaggaagga
     1381 ctaacttgtt tgatttatcc gttgtcaaag aagtggacgt tcgagagtcc ctaatcgtat
     1441 agcagtcatt cggcgagtat aacctaaaac ataacctgcc aattgccaag ggtaaaatgc
     1501 acgctgcgcg cccttggcaa tcgtcagaac atgttttcga gccggttatg ccgaacgacc
     1561 gctatacgaa aagggcctct aaacagaagt ccactttgat ccttgacaac tgaaacccaa
     1621 aatcaaacaa gcaggccttc gtctaaaaac gagccgaatg aagaccttgg gggttgcacc
     1681 cccaaacccc cgtgcccccg acacactacc gaaaaggtgg gggtgggtgg cgaaaaaatc
     1741 aaataaaaca ttctattcaa gaaaggaaga aacaaccatg acaactaaaa acgagcaact
     1801 gaaagaacag gcaagaaaag agttgcaaga aagaggtttg attattgagg ggagctttga
     1861 aggagatttt gaaacctata ttggttgtta tgcaagaccg ataaacaaac ctacagcctt
     1921 agacccaatg aacgaacaag aagtaaaaga acaagaaaaa catgcagtga atggttttcc
     1981 gcaagatttc actgaatggt atgagtggga aattaacaac gggaagcttg aaaacttctt
     2041 ataaaagtca gatgacgtgc aaagagaatg attctctttg tacggtaaaa atataaagaa
     2101 agaaggaaaa agaaaatggc aaaaaacaca ccttatcaag taggagacat tttttatagt
     2161 agttggggct acgaacaaac gaacgtgact ttttggcaaa ttgtgaagtt aacagaaaaa
     2221 acagcatggt ttcagcctat tcaaaagaaa acggtcgagc attattcgtc catgagtgga
     2281 aaagttgttc caatgaaaga caagtttata gagtatcctt tagcaaaaac taaagacagt
     2341 attgtaaaaa aacgaattga tccagaaacc tatcctgata tgttatgtgg ttatcgtagt
     2401 tgggacacgt ttcatagata taaaaatcaa gctgtttatg aaagtagtta ttattaaggg
     2461 gatagtttcc cctttcaaaa ataataaata gaagaatgga gagaaaaaat catgatgaat
     2521 tcagtgaatt tagtaggacg gttaacaaaa gaagtggatt taaaatacac accaaaaggc
     2581 aatgcgacag gcacatttat tttagctgtt aatcgaaatt atacgaatgc aaacggcgag
     2641 cgtgaagcag attatatccg ctgtgtcatt tggcggaaag ctgcggaaac tttagcaaaa
     2701 tatactggaa aaggaagttt gattggcatt aatggacgtc ttcaaacgag gagctatcag
     2761 aatcagcaag gacagaccat gtatgttacg gaagttctag tgacagattt ctatttgtta
     2821 gagagtaaag aaatcaatga gcaacggtca aaatcaatgg cgaataatca aaataattct
     2881 tcaattccag gaaatgagat tcaaatttca gatagttatt tgccctttta agtgaaaaat
     2941 aattttgttc tatacctgat ttcttcaggt atagaacaaa aaataaatag agatgaggcg
     3001 attttttatg aaaatcattg acagttacga tcaacgaacc atgcaatatt tgggctcgtt
     3061 tgattataca gatgaaaata ttattaatta tgttgcaagt ttacctcctt atcaaagcgt
     3121 tcgcttggta gattttgcaa gtgatgaatt ggtattaaca acgatgggca actttttaga
     3181 ttatgtcgga gacaaaaaat ggttggaaaa aattttgtct aaattgattg cgaaacaaac
     3241 aggagaagaa cccataaatg cgattcaatt gttcaatcga tatgaacaag aaaacaatga
     3301 ttaaagagga gagaaaaaat gaaaagatta aaacgaagta tttttattgt gctagcattg
     3361 attgattttg ttgggacagg ttgtgaagca attgacgttc aaaaagctcc cttatcggaa
     3421 ataaaacaag caataacaaa acaagatgaa gaacaaccgc taaatacaca agtagaaata
     3481 gtgaatcaaa ataaaccgag tttttctgaa gaagattttt cgttagaaaa tggaaccagg
     3541 caattttttt cagatttgga tcgtttaaat cgggtaggtg tagcaagtgc acttttgcat
     3601 aaaagcagga tgccaacaga agaacgagac cgattataca ttaagccaac tggctggaaa
     3661 caaaagaaaa tgaagaatgg cgattatctt tataatcgtt gtcatttgat tggctatcaa
     3721 cgtgcgacaa gtttccccat aagtatgtaa tacgaaacgg gaggattaat cttaagatta
     3781 atcacaacta acctatttga cagttggaat gaaagctatc atgtctgctg aatcaactgg
     3841 tctgatactc ctacatgcaa taaagtctac taggaagtta ttgtatgaag ctaggtaaag
     3901 gcggcagaac aatttctaag tgtggaatca tatgaagttg gatatgccgg gacgctataa
     3961 attactcagg gtgagactgt ttttaattaa actgacgaac ttgcgaatgt acgggtctat
     4021 aaccgactta tattcgaaag atataagatt gcgaatgcaa agaacattat cgaaaagtaa
     4081 gaatgttggt catgaaattc gagatggaag actagcggtg tatctgtcaa gagttggtaa
     4141 aacatgtttg attgattcag atgagagtgg gaatgcgaca aaacgtttta cagaagaaat
     4201 agagcaacta gcagaactga atatacgata tttttaaata ctttagcgga gaataaaaaa
     4261 atcctctaca gaaaactacg gagggcaaca tgtgctattg tttcaacaat acatttttaa
     4321 tttttaccat aacaaaaaag tacttgaaaa aaaacagaaa tatgttaaat ta
//
LOCUS       DACDSY010000179         4312 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDSY010000179 DACDSY010000000
VERSION     DACDSY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.182"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..182
                     /locus_tag="IWK44_002658"
     CDS             <1..182
                     /locus_tag="IWK44_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9551121.1"
                     /translation="RKTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSR
                     AQRRFEGTLRYSLGY"
     gene            214..936
                     /locus_tag="IWK44_002659"
     CDS             214..936
                     /locus_tag="IWK44_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9551122.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            994..3033
                     /locus_tag="IWK44_002660"
     CDS             994..3033
                     /locus_tag="IWK44_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP9551123.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3142..4293)
                     /locus_tag="IWK44_002661"
     CDS             complement(3142..4293)
                     /locus_tag="IWK44_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9551124.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1554 a          795 c          844 g         1119 t
ORIGIN      
        1 aaagaaaaac agttgagact gttttttctt ccttagaaaa attaggttgt caaaatttca
       61 actcacgttc tgttaaaggg ctagagagta gatttgaaag catattactg gcttacagtg
      121 ttctattaag tcgagcacaa cgacgttttg aaggaacttt gagatattct ttaggatatt
      181 aaaattaact agcaccacgg gttattatct attttgttac tgcttacatt agtagttggc
      241 tatttttcga ttgagtttat ccatggattt tcgtctgcaa aacagagttc aactgtgaaa
      301 aaggtagagc cgaaaagtat ccctaccaca ctaaatgtgg ctttgattgg ttcagatgct
      361 cgttcgaaag acgaaaacgg tcgctcggat tcacttctgg ttgcacaata caaccagaaa
      421 acacaacaag caaaactgat ctctatcatg agagattcat atgtcgatat tccaggttac
      481 ggaatgaata agataaatgc agcttactct tatgggggag ttgatttatt aaatcaaaca
      541 ttaaaggaaa atttcaaatt tgaagctccc tattatgcaa gtatcacatt tcaagatttt
      601 atcgattgcg ttaatgaact gtttcctggt ggagtcaaga tcgatgcaga aaaatctcta
      661 gatttagatg gcgtatatat aaacaaagga gagcaagtga tggatggtaa tactttactc
      721 cagtatgctc gatttcggga agacaaagaa ggagacttcg ggaggatcag aaggcagcag
      781 caagtgatca aagctatctc tcagcagctg aaagacgtaa cttcaatatt caagctaccc
      841 aaagcggttg ggaaattact tggtagtatc caaacgaacc ttccagaaag tgtgcttctg
      901 gattgtggta tggatttcct caaagacata tgttagaata aacaggtata aatagtgaaa
      961 taaaggaatg acaagcaaga gaaggaggaa aaaatgaaaa gaagtgacaa gcacggcaaa
     1021 aatcgaacag gcgcttatat tgccggcgca gtgatcttaa tagcagctgc gggtggcggt
     1081 tatttttact accagcacta tcaagaaacc caagcagtag aagctggaga aaagacagtt
     1141 gagcaatttg tccaagcttt gaacaaggga gattataaca aagctgcaga aatgacatca
     1201 aaaaaggcgg caaataaaag tgcattatct gaaaaagaga tcttagataa ataccagaat
     1261 atatacggtg ctgctgatgt caaaggactt cagatatcga atctaaaagt agataaaaaa
     1321 gacgattcta cttacagttt ttcatataaa gcaaagatga atacctcatt aggtgaattg
     1381 aaagatcttt cttataaagg aacattagac agaaatgatg gtcaaaccac gatcaactgg
     1441 caacccaact tggtttttcc agaaatggaa ggaaatgaca aagtaagcct gaccacacaa
     1501 gaagcagcaa gagggaatat tatagatcga aatggtgaac cattagcaac aacaggcaaa
     1561 ctaaaacaat taggagtcgt tccaagtaaa cttggagatg gaggcgaaaa aacagccaat
     1621 atcaaagcga ttgcttcctc cttcgactta acagaagatg ctatcaatca ggcgatttca
     1681 caaagctggg tacaacccga ttactttgtc ccattgaaaa tcattgatgg agcaacgcca
     1741 gaacttccag ctggagctac cattcaagaa gtagacggca gatattatcc tttgggtgaa
     1801 gcagctgctc agctgattgg ttacgtggga gatatcacag cagaagatat tgataagaat
     1861 ccagaattaa gcagtaatgg aaaaatcggt cgctcaggtt tggagatggc ttttgataag
     1921 gatcttcgtg ggacaacagg tgggaaatta agcattacag atgcagacgg tgtagagaaa
     1981 aaagttctga tcgaacatga agttcaaaat ggcaaagata tcaaattgac aatcgatgcg
     2041 aaggcacaaa aaacagcttt tgacagtcta ggaggaaaag ctggatcaac tgttgcgaca
     2101 acgccaaaaa ccggtgatct tcttgcgctt gctagctctc caagctatga tccaaacaaa
     2161 atgacaaacg ggatctcaca agaagattac aaagcttatg aagaaaatcc tgaacatcca
     2221 ttcatcagcc gatttgcgac aggttatgct cctggatcta cgtttaaaat aattacagca
     2281 gcaatcggtc tcgacaacgg cactatcgat ccaaatgaag tgttgacgat caacgggctt
     2341 aaatggcaaa aagacagttc ttggggatct tttcaaacaa cgcgtgtcag tagtgatgta
     2401 tcacaagtag acttaaaaac tgctttgatc tattccgata atatatatgc ggcacaagaa
     2461 acgttgaaaa tgggtgagaa aaaatttcgt acaggcttag ataaattcat ttttggtgaa
     2521 gaccttgatt tgccaattag tatgaatcca gcacaaattt ctaatgaaga tagctttaac
     2581 tcagatatct tgctagctga tactggatat ggacagggcg aacttctaat taatcctatc
     2641 cagcaagcag caatgtattc tgtttttacc aacaatggca cacttgtcta tcctaaattg
     2701 attgcagata aagagacaaa agataagaag aatgtcatca gcgaaacagc agtacaaacg
     2761 atcgtgccag atctgagaga agttgtgcaa gatgtaaatg gtacagcaca ttctctttct
     2821 gctttaggga ttccgttggc agcgaaaact ggtacagcgg aaatcaaaga aaaacaggat
     2881 gtaaaggggc aagagaacag tttcttgttt gctttcaatc ttgataacca aggttatatg
     2941 atggtcagca tgttggaaaa taaagaagat gatgattcag caaccaaacg agcatcggaa
     3001 ctattgcaat acctcaacca aaattatcaa taaaaaattg ctttttaagt acagtaaaag
     3061 gactgtggcg ttatcgtcac agtccttttt tcaaataaac ggttttcacg atgtctcttt
     3121 cacaacttct ttttgattta ctcacacttc ataaactttt tttgtaaaat atttgagaac
     3181 aagtggagaa ataagtgtac ttaataaaat cacgataact aaaggtgaat agtactgtgg
     3241 ttcgatcaat tgactttgct gtccaatttg taaaatgatc aaagccattt ctccacgcga
     3301 aatcatacca gcaccgacca tcaatgaact atgttgagaa aaaccggcaa tctgagcacc
     3361 aaaataacct ccaagcaatt tggtcaaaat cgctacaagc gtcaatatag aaataaagag
     3421 tagttgttca ctaaagtcag aaaaatcaac ttctaatcct acactgacaa aaaatactgg
     3481 tataaatact gcatatccta aagcctctac attgtggtat acttcttctt taacctttgt
     3541 ctgactgaca gctatcccag caaaaaaagc accaatgaca gagctaagcc caacaagatc
     3601 cgctacgtaa gacattccta aacaaataac tagcgatacg atgataatag aagaatttgc
     3661 gtacactttt tctgcaaatg aaagtaggaa aggcgcaatc catttgacta ataagaaaat
     3721 aaaaacaaaa taaattagct gttcaatcaa aacaagcggt aaagaagcat tgctagaagc
     3781 ttcccccgtt agaaaagata ggctgaaact tagaactaaa acgactaaaa tgtcatctac
     3841 cacagaagca cccaaaattg ttgatccttc ttttgtattc actacgttta gttcttttaa
     3901 tacttctact gaaatactga cagaagttgc cgcaagaata atcccaaaga aaatcgcttc
     3961 atttcccgaa acttggaatg ctttgcctgt caaccagcca aaaacaaccg ggaagatgat
     4021 cccaagcaaa gcaacgaaca taccaggtcg gaaatatttt ttcaaaagag ataaatcact
     4081 ttccagacca gctaaaaaca ttaataagat cacaccaatc tccgaaaagt catggactag
     4141 aatatcaggt ttgacccaat tcaatccagc ttggcctagc aaaacaccaa caagtaattg
     4201 tccaatcact gctgggatac ctaatctccg cgagatatgt gagccaatcg ttgttgaaat
     4261 caatatcagg cataaaatac caataaattc cattctcatc attcctttgt at
//
LOCUS       DACDSY010000180         4312 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDSY010000180 DACDSY010000000
VERSION     DACDSY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.183"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..858
                     /locus_tag="IWK44_002662"
     CDS             <1..858
                     /locus_tag="IWK44_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP9551125.1"
                     /translation="QNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            875..1309
                     /locus_tag="IWK44_002663"
     CDS             875..1309
                     /locus_tag="IWK44_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551126.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1926..>4312
                     /gene="esp"
                     /locus_tag="IWK44_002664"
     CDS             1926..>4312
                     /gene="esp"
                     /locus_tag="IWK44_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP9551127.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
BASE COUNT         1584 a          598 c          816 g         1314 t
ORIGIN      
        1 caaaatttga ctttgtattc tttaggagac attcgagata ttctgatttt agttcattat
       61 ttttttactg gaaaaataga agatctattc catgaaccgt taattgaata tacagaaaat
      121 ctaagtaaaa ctatcgagca aatcaaaata gataatctgt tatcacaaaa ttacgatcca
      181 gaaatatatc tgtttcttta tgaaaataaa atactggaat atgttaaaaa tggagatatt
      241 cgaaatttag aaaacatggt ttttaatctg agtaatggaa taattccaag tgttagtgga
      301 gatacgattc ggtctgaaaa aaattattct atcattgttt ttgaaaagtt agcacaaacg
      361 tctataacat taggaatgga tataatagaa gcgtatcaaa gtagagatgc tttgatacaa
      421 gaaaacgaat tagctgtatc gttacctgag gtcttaaaag tgagggattc tgggattgtt
      481 tattatacta aggaaatcgg aaaaactaag atagagcatc tttctccctt aatttcttct
      541 gtcgttcaat tcatcggctt aaatatctat aaaagaatta ctgtaaagga aatagctaat
      601 tatttttctg taagtgaaac taaattaaga gaaagcttta agaatgaaat gcatattacg
      661 atttataatt atatctctaa gcgaaaaatt tctacggcta aaataatgtt gaaatcaaac
      721 catactatca gtgaagtttc tttaggttta ggattttcag attcttcaca cttttcgaga
      781 gtattcaaaa agtacgcagg tgtatctcct aagcaatatc aattaggttt agtggacaat
      841 ttcaacaaca ttagctgaga ggttttattt tattatgaga ctatatcatt tacgtagttt
      901 ggaggagttg aggaaattgg attttaacaa aaaatttttt tgggaaattt ctgatttgag
      961 ttgtttcgta gaatctgagc gtctaagtaa tggtaataat agtgtgttaa atgaatatga
     1021 caaagtagta attaataaag taaaaagttt atgtgaagaa gctagagaat ctatcagtca
     1081 tattaatggt agcaatttta aacaaacatt atttaatctg attaagctag atgctaaaat
     1141 atcaagttat ttattctttt tgatacatga tgaatttatg gattatcaaa aattggatca
     1201 gttaattgaa agagaaagct gggaagatta ttacgtgggt ctaacttttt cagaaaagtt
     1261 aaacaactac aagttaatag attataagta tttgagtgaa agcaattaat tattcatgta
     1321 gagtactaga cactttaaat atggacaatt caattataaa gaaaaagaat caataattta
     1381 atgaggctgt gacaaatgtc attttttgtt tcgctaaaaa cgagtgaaaa aatgttgata
     1441 actccaatca ttcgtttaag attctaggaa tcattcagaa atgctgtgaa accgatactt
     1501 tataattata aagaaggtga tttcaaattc cgaagaagga atttgaagtc acctttttac
     1561 ttatgtcaca gtctcattca gtaatgcggg ataaggctcc ttattccgtt atttgaatac
     1621 tctgaagttt aatctaagaa agggtaatat tttgaaaaca tgcttcatcg catagaaatg
     1681 aacttaattt cagaaagaat acatacatat ctttttatat gttttcagct gctgaatggt
     1741 taaaaagcac aaatatccga aaaaaacatg aagaaatgta ctttttttgt tgtaatgttc
     1801 atgaaattct ctaagtaaat tagttaacat aaggttagta agattcctat tctgtgaata
     1861 atacgtatca gatttatctt ttattcttac taggaaagta attattaagg gagttgattt
     1921 gaataatggt tagcaagaat aataagagag tatttctaga aaaaacaaag aaacgagtat
     1981 tgaagtacag tatcaaaaaa ttaagtgttg gggttgcatc tgttttgatt ggtgttggtc
     2041 ttgtcttggg aacaactgaa ctagttaaag cacaagatga aatttctcca agcagcccac
     2101 ttgaaacagc gataagctcc gtacaagtag gtgacaaagt agcttcgggg aatacttttc
     2161 aggagaatcc tggttatacg aaaaattata acttttcgga tttacaattt agtcctcaag
     2221 aactaactgg agacacacta aaaggcaata caattggatt tgaagtttac ggaaaacata
     2281 atattgcagc atcaactaaa aactgggaaa tccgtcttca attagatgaa cgattagcta
     2341 aatatgtaga aaaaattcag gtcgatccaa agaaaggaat tggatcttct agacggacct
     2401 ttgtaagaat taatgattcg ctgggtagac ctacaaatat ttggaaggtc aattatattc
     2461 gtgcaagtga tggattattt gctggggcag aaacaaccga tacgcaaacc gctcctaatg
     2521 gggtaattac gtttgaaaag agcctagatg aaatttttaa agagataggc atagacaatc
     2581 tcaagaccga tcgtttaatg tatcgaatct acttggtaag tcatcaagac gatgataaaa
     2641 ttgtaccggg tattgatagt acgggctact ttttaacaga ttcagatgat ttctataata
     2701 gcttagatgt atctgaaaat aatcctgacc agtttaaaca tggatcagta agtgcaaaat
     2761 atgagaaacc taatactcaa acaaaagatg gttcaggatc cacaggtgct aatggagcta
     2821 taatcttaga tcataagtta acgaaaaatt ataatttctc atattcatct tccgcaaagg
     2881 gcacgccttg gtatgctaat tacaaaattg atgaacgatt agttccatat gtagcgggta
     2941 tacagatgca catggttcag gctgacaaag taacatatga tgtttcgttt gaatccggaa
     3001 caaaagtagc tgatttagca atcgagcgac gggaaggtca tgaaaattat ggcataggtt
     3061 caataactaa taatgattta actaaactta tcgattttgc taatgcaagt ccacgtccag
     3121 tcgttattcg atatgtttta caattgacta agccattaga tgaaatctta gaagatatga
     3181 aagcaacagc acaagttgaa gaaaataaac catttggtga agatttcatc tttgattctt
     3241 ggttgtcgga tacgaataaa aaattaatcc agaacactta tggaacaggt tattattatt
     3301 tgcaagatat tgatggtgat ggaaaccctg acgataaaga agagagcgga gacacgaatc
     3361 catatatcgg gaaacctgaa ttagaagaag tatatgatgt tgacacaaca gttaagggga
     3421 aagtattcat ccacgagtta gcgggaacag gtcacaaagc ccaacttgtt gataaagaag
     3481 gtactgtatt agcagaaaaa actatcgctc caaatgaaaa agatggggct ccaatttcag
     3541 atactgtaga atttgaattt acgggtgtag attcaagtaa attaatcgcg aaagatgaat
     3601 taaaaatcca aatcgtttct ccaggttttg ataaaccaga agaaggttca accgttatta
     3661 aggaatcacc aaaagcggtt gataaacaaa ccgtggtagt tggatttaaa ccagatgcta
     3721 aagaatcaat tcggaataat aaaaacttac ctgaagatgc agagtattca tggaaaacag
     3781 agcctgatac ttctaacgtt actgatagta cgaaaggtat tgtaactgtt aagatcggaa
     3841 atcgaacttt cgacgtggat gtagagtttg ctgtaaaagc ttctcaagct atggaaaatg
     3901 atgcaacata cgtacctata acaacaactc cagaaacgac agttcaaagt ggaaaaccta
     3961 catttgataa accagatgtt cctttagcta aagatgcctt ttcaatttca gatgtctata
     4021 ataagtactt cggtaatgca agtgttgatg caaacactgg tattgttaca ttcactccag
     4081 ctaaaggtgt aggagaatcg gagccgatta ctggaacaat tcctattaaa attgtttacc
     4141 aagatggttc tgtaggcacg accgatttag cagtaactgt aagtaaagat atttatgaaa
     4201 atccaggaga aaacattcct gcaggctacc acaaagtaac cttcaccgca ggggaaggaa
     4261 caagtattga aagtggaaca acagtctttg cagtgaaaga cggcgtaagc tt
//
LOCUS       DACDSY010000181         4309 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDSY010000181 DACDSY010000000
VERSION     DACDSY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.184"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(221..1423)
                     /locus_tag="IWK44_002665"
     CDS             complement(221..1423)
                     /locus_tag="IWK44_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9551128.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="IWK44_002666"
     CDS             complement(1426..1620)
                     /locus_tag="IWK44_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9551129.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="IWK44_002667"
     CDS             complement(1667..2515)
                     /locus_tag="IWK44_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP9551130.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="IWK44_002668"
     CDS             complement(2720..3070)
                     /locus_tag="IWK44_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551131.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="IWK44_002669"
     CDS             3665..4111
                     /locus_tag="IWK44_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9551132.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
BASE COUNT         1255 a          740 c          807 g         1507 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagttt
//
LOCUS       DACDSY010000182         4218 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDSY010000182 DACDSY010000000
VERSION     DACDSY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.185"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..369
                     /locus_tag="IWK44_002670"
                     /pseudo
     CDS             1..369
                     /locus_tag="IWK44_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(495..1574)
                     /locus_tag="IWK44_002671"
     CDS             complement(495..1574)
                     /locus_tag="IWK44_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9551133.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(1677..2117)
                     /locus_tag="IWK44_002672"
     CDS             complement(1677..2117)
                     /locus_tag="IWK44_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9551134.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2174..2584)
                     /locus_tag="IWK44_002673"
     CDS             complement(2174..2584)
                     /locus_tag="IWK44_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9551135.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(2764..3198)
                     /locus_tag="IWK44_002674"
     CDS             complement(2764..3198)
                     /locus_tag="IWK44_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9551136.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(3298..4146)
                     /locus_tag="IWK44_002675"
     CDS             complement(3298..4146)
                     /locus_tag="IWK44_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9551137.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1509 a          792 c          692 g         1225 t
ORIGIN      
        1 tccagaagaa aaaccagatg tgactccaga accagatacg gacgcaagag accaaggaat
       61 accagaaaaa ataaacaaaa aaacaattca agaggatgga aaaaaagaga gtaaaaaatc
      121 aaatctagca attctaaaaa taaatgaaga gcagctgaat aaaaaatcac gaatatttga
      181 tagcgcccaa tcagctgaaa ccttaaaaag tagcaaggat acaacctttg ctgtcctgaa
      241 acgaaaaata aacagctacc aaaatccgga gagtcacaaa acaaagttat actgtggagc
      301 ggaatcattc tattaagtat agcaacaatg ctttctgcaa aaagatttaa acaaaacaga
      361 tctctctaac atttgaataa tgtctgtgaa cgcatttttc aaataaaaag aaatatccag
      421 aaaatgaccc caaaatacca gattgaaatc tgatatttgg ggtcgttcat actttatctt
      481 tcttcatatt tattttaagg gatatctgtt tcttttggaa gatggttcgg gatctttttc
      541 actttcaata catctaaaaa gaaggtaaag atcggaatgc cgacaatcaa gccccaaacg
      601 ccaaagaacc gttcgctaac aagtaagacc acaaatgtat aaaaaatcgg cagttctgtt
      661 ttgctggaca tcaacttcgg attcagcaca taagactcaa ttagatggac aatcaaaatc
      721 gtaatcagga tataaacaac atctcggatg cctccttggg aataagctat gaaagataga
      781 ggaatacaag aaatgataac acctgctact gggatcagac ttaaaatgaa aatcatgatc
      841 gctaaactca gcagctgatg aaagccaaat acagccaagg cgattgtagt gagcaacgta
      901 tttaaaagag cgacaacaaa ttgagcttct aataccaaac caaatgtatt gacaaactta
      961 tcggcaaaat agtaaatatc ttgaaagaac caagaaaatt cactttttaa aaacaatctt
     1021 gagaatacaa ttgttttttt cttttcaatc ataaaaaaga aacttaagat gaatgacata
     1081 gcaaacgcca ctgccatttt tcctatatcc tgtaaatagc caagcgcaat cgaagcgcca
     1141 ttttgaagct gttcaaaaaa atcgtttcgc tcaatataag tatggatata ttgtaaaaca
     1201 ggatctgcat cttttggctg atgctcgtaa aaattaataa cggactggat catgtcaaat
     1261 gtttgatgaa taagcatcgg gatatacttt gtgaatgcca gatagataag gtaaacgatc
     1321 agtgtataaa tcatgatagc taaaacacct gtagggattc ggacaaatcg ctgaatgatt
     1381 tgaacaagat gaatcatcaa ataagtaaag ataaatgtga gcaaaatggt tgtaatcata
     1441 cttctaacta agaaaagaac aaaaacgaca aataataaaa cagtgagacg gcgtattttt
     1501 tcattcgcca aaaaacgctg ataaacggtc aaatcgacaa cctccaaata acaaaaaata
     1561 aacacgttat tcattatatg atggaatatg aaaataagaa agagcaaaaa gaaaaaccgc
     1621 taaatgacgt tatcttatga gttggttttc ctcataaaat cgttcatcta gcagtttcag
     1681 ccctttttct ggtcttgatg aaaattatct agcatttttc gggtcagatg ttgcagtgta
     1741 tccaattctt cctgttcaag tggaagataa gacttgatct tttgtggaat accttctgct
     1801 tgtttttgca acgcagcacc ttgagcagtc agcgtaacat ttacgattct ttcatcaccc
     1861 gtttcacgct gacgattaat ataatttttt tctgctaact tttttagtaa gggagtcagc
     1921 gtaccagaat ccagacacaa atcatttcct agctctttca ctgaagtctt tttcttttcc
     1981 cataaaacta acatcacaag atactgggta taggtaagat tcagcggctt gaggagggga
     2041 gtgtagtgtc taatgatttc tttagaggcg gcatataaag gaaaacatac ttgattaact
     2101 aaatatagat cttccatttt tccacctccg ctgttttact atagtgtatc atagcggatt
     2161 tacacaaaaa agtctattca ctaactgctt tcagagtaac ttctatattt ccagctgtag
     2221 cttttgaata cggacacact ttatgcgctg cctctaataa ttcttgcgtt tcttccagag
     2281 aaagaccttc gatagatcct tcaatctcta cacctaagac aaagctcgtt tcactttctg
     2341 aataaaggga aacttgtgca ctgacagtag aagccccaga gatatttttt tgctgtttga
     2401 tcaattctaa tgcactgtta aaacatgcac tgaaaccagc tgcaaacaat tgttcagggt
     2461 tggttgcatt ttcgacgcgt ttaccaggag ctgcaatatt caattcgaat gtacgatcgg
     2521 gattatgcac ttctccagag cgtccgcctg tgttgatcat tttcgtagaa tacatttttt
     2581 tcataaaatt accaccttta attatatttt atacaattga attttacaca atataattgt
     2641 ggaaagcaaa gaaatggatt ggcataagat aaataagaga aagcacatga aacagacgcg
     2701 ctttctcgaa tagcaaacat atacaaaaaa tatcagagac aaaaaataat ctcatcataa
     2761 acattacata tatttctcaa aaagttgttt tctttcttca aaaattactt cacgatggac
     2821 ctgatgaagc atccataaat aaccaaaagg atctctgaac attgagttag tcacaccaaa
     2881 gtccatcatt tctgtcattt cttgaatctc tacacagcca tttcgtaatg ctttattatg
     2941 tgtgtcctcg atatcagaga cgatgatatt gaaccagatc gtatttggat gttcagcagt
     3001 aggagcagtc aaaccaagag acgtgttttc atccagcata tggaatctcg taccatacaa
     3061 ggtaaaaatg acttcattat tgcctctagg gtaatctgtt gcctcaatca gttgtatttc
     3121 atcaaagatt gtttgataca gttctagtgc tttcaaacta tcatttaata caaaatcgat
     3181 ttccgttcct ttatgcataa agatctcctc tccttttcag tcgaacagtt actagattct
     3241 tttattatac cagctaaaga aaacgcattc aaaaaaatag cggatttact taccaactca
     3301 ttcgattgga tgataagtaa atccgcgtcc cattacttct gctacgctgc tgacaatgac
     3361 aaaggcactt ggatcgattt cttctatcaa tggtttcatt cgggaaaatt catgatcagt
     3421 cacaacgatc aataaaacct cttttgactt tcgattatac ccgccttttg cctctagcag
     3481 ggtaaggcca cgtccaatct cagcacttaa gcgaaggcgg atgtcttcca aataatgttc
     3541 actcatgacc ataacagatt tctttcgatt gatacctgtt tcgatataag tcatgacaat
     3601 tgaagtgatt gcaatagata aaatagcata aagaaactct tctattccaa aaataaacag
     3661 attaagagaa acaacgaatg cgtctgtaaa aaataagcca atcgaagtat tcaagtggaa
     3721 atatttttta aaaatgaggg gagggatcgt cgtaccgccg ctagaagagt tgtttttata
     3781 taaaatcgca actcctaaag caaaaatcgc acttccaaaa atgacagaca acaaccgatc
     3841 tgaagtaatc attgtttctg gaactattgc aatgattaaa ggaaaaacca agcttccgaa
     3901 aagtacttta aaaaagggtt ttttacctaa aaatagaaga gctagtacca acataacgat
     3961 attcaacact aaaataacta tagctcggtc aaagcctaaa gcagcttcca gtaatattcc
     4021 tagaccgctg acacctccag cagcaatatg atgaggccct aaaaacatat tgatagcgac
     4081 tcctaatatg aagagggcaa aaataatctt aatgtatgtc catataaatt gtctcgtttc
     4141 tttcatttat tcacttcctt aattcattaa taatactctt atcttattat aacttacttt
     4201 tcaccttaag ttaacttt
//
LOCUS       DACDSY010000183         4130 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDSY010000183 DACDSY010000000
VERSION     DACDSY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.186"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            46..1347
                     /locus_tag="IWK44_002676"
     CDS             46..1347
                     /locus_tag="IWK44_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9551138.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1374..1634
                     /locus_tag="IWK44_002677"
     CDS             1374..1634
                     /locus_tag="IWK44_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9551139.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2633..3868)
                     /locus_tag="IWK44_002678"
     CDS             complement(2633..3868)
                     /locus_tag="IWK44_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9551140.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1188 a          666 c          861 g         1415 t
ORIGIN      
        1 tcatgcaatg aatggttcat attttattga aaaggagtgg ttttagtgga aaagctgatg
       61 aaatgggtcg aagagaaact tgtcccacca atggcaaaaa ttggtacaca gcgtcattta
      121 ttagcaattc gtaatggggt tgtttcaaca ttgtcattga ttttgattgg gacatttttt
      181 atggtcttta tcaatttacc gtttcctgga tggaatgaat ttattgcccc ttattcggca
      241 acgattgttt taccctttag gatgacgatg ggattgatgg ctatttatgc aacttttgtt
      301 atgggttcgg atctagctaa atcatatgga ttagatagtg tgacaggtgg tatattatct
      361 ttaggaacgt tctttatgtt acaagttcct gtaaacgttt taactcctga agaggcacct
      421 cttggctggg tcttacctat gagttctttg ggagcatctg ggatgtttgc ggggatctta
      481 tctatgattt ttgcagtgga agtatatcgt ttctttaaaa atcgaaatat tactatcaaa
      541 atgccagaac aagtgccacc tgctgttgcc cgtagttttg aggcattgat tccaggtgca
      601 gtcattttaa caattacttg gctgattcga agcgtcgcgg gctttgatgt aaacgctgcc
      661 ttattatcgt tattcgagcc tttaaccaat attttaggga ataatttgct tggtgtttta
      721 ctaccaatgt ttttgatcca tttgttgtgg tcatttggta tccatggcat gagtattgta
      781 ggggctgttg ttcgtccaat gtggctgatc atgttggatg agaatgcaaa ggcattggca
      841 gatggtacag ctgccaccca attaccctat atcgctcctg aacagtttta tcaatggacg
      901 gtaacaattg gtggtgcagg agcaacgatc gtggtatctg ttttgtttat attcttctgt
      961 aaatcaaaat tcttgaaaga ggttggtcgt ttttctatta ttcctggtat cttcaatatc
     1021 aatgagccca taatttttgg agctcctatg atgttgaatc cttacatgtt catcccgttc
     1081 aacttagttc ctttagtttt gactattatc agttattttg cagtcaaatt agaaatggtc
     1141 aatagattta ctgttttgcc aacatggaca ttaccagctc caatcggcgg ttatctttca
     1201 gcaggaaatg actggcgtgt tgttatcttg atcatgatca acacattgat agcatttatt
     1261 atctattatc catttgtaaa agcttatgat aaaaagatgc tgtcagatga acaaagtcgt
     1321 ggagaaaacg aagtccagac tgtctaagtt tggtttttag aaggtgggaa acaatgtatt
     1381 ggctgatttt attttttgtt tttatctttt tattaacggc tagtcacctt atattgaata
     1441 tgctagctac atatcatatt caaatcaatc gatggatttg ggcactggca tcatttttga
     1501 tcgtgattct tccaaagatc attgtaccgc atatgaatgt tttgttttca tgggggacat
     1561 atgtactttg tggaattttt gctatcaatt ttatgatcga gcagcataga tggtttgtca
     1621 catcgaagct ttaaatgttc taaaagtggt tgtaacagaa gtggttagct tcaagaaata
     1681 agaagaaatt tacgaaaatt gcttcttaaa tttttgtgaa atgttggctt atttttcgtt
     1741 tggttgaact tattaccgca gatgcaagct tttgaccacc gtttattcgg aatatgggac
     1801 tgtgacaagg cttttgtcac agcccttttt tacataaaga ttattattgt tttaactgtt
     1861 ttggcagaaa gaaggaggaa aaaatggaaa ttttatcagc aatggattta gaattagtag
     1921 atctactacc aaagtgatca agtcaaatat agatttggga gcttacaacc atatagtagg
     1981 ggctgcagta tggtgtatga atggtaatat gtatgatgtg agtgtttatt caaatcatga
     2041 aacgtgtata gaaataattg tgctgggaat agcaatcagt catggtgaac gtgactttta
     2101 gtgtatcgtt gctgacgaca tagaacattc tgacatgatc tactcaatat gtggggattg
     2161 ctggcagttt atataggagt atgcatcgga ttgcgagatc attgtctcaa cagaagaggg
     2221 aggttgatta tcaaagataa agtggtaaat cctacatttg atttagtaat tgttgagggg
     2281 actagcggca caaatctcta caaagcgatg cttctagatc tatatgacac attttaagaa
     2341 aatgtatttg cctaattatt atggtctttc ttttgaagaa acgttgagat gatccaacca
     2401 gagaaaggtg tcaaagaatt tgactaagaa taaatggcag acgctaccta tgctcatttg
     2461 gatagcgtca gatcttttat tttaaggatg attttgtgtt ttttagacac cgtaatttaa
     2521 gtaactctaa cttttcaatc cactatttac tttttttgta ttttcactgt ctgggtctcg
     2581 aatagcgaaa ttactgatga gtctctatta aaaaagcttt ttctacttga aactatattg
     2641 ggtggttgct caggaaggtt tcgatttctt gaattgcttg ttgtggacca tttattggaa
     2701 aaacggcttg cagcgtgcgt gcctgctgac gaatcttggg tgaagaaact tcgcgcagag
     2761 caatctgcag ttttctttca gtcactggtt ttgtgagggc gattgcgttt ccgtatttta
     2821 cacaatagtc aatattacag ctttgctcaa attgcatccc cacaccgata aacggtttgc
     2881 cggattcaca ggcagtttgg acggtccctt ccccgccgtg gataaaagaa aaatcaatga
     2941 attcatgcaa aagatgacta gacagatatt ctgttattaa aagatcttct gagtcagatt
     3001 gaatgtcatc aagcagatag tcaataggtg caataaccgc tagatccggt tgtttttgta
     3061 agaagaacaa aattttgcgt aacatacgcg gattgcctga actacccatt gcgaaataga
     3121 taatacgttt tctttcttgt tgctttcgct gtataaaagt tagcacttct tcaggcaagg
     3181 aagtactgag cttagcaaaa attggaccaa cgattcgata attttccggc agctgttctt
     3241 cttccaaaaa tagtttaggc gttgtgatga gatttagatc agcatccatc atttgtaaac
     3301 ttgtttttgg taaaggaagc atttcttttt gtgcagcgtg tttgaaggca ttggggatcc
     3361 atcgggtatt tactatacgc ttttttgtcc agtgccaaag gggattgctt aaagggccga
     3421 agaaacgtaa ctcgtggggc atcagcgcat gctctgattg aaaaaaaggt tcactcaaag
     3481 catagggttt aacatatact aaaggaatct ttttattgcg ggccgataaa aaaattgtca
     3541 cattcgaacc tgtcacaatc agtcgaggct caaacttagc aatcagactc tgttcactag
     3601 tgatacgaga ggcaaccatt tctttggtaa aagggttttt aagtgtacgc aactggtcaa
     3661 attttatgat ttgttcttct tgttttttcg taaggatcgg cgctagtaaa tgatactcaa
     3721 agccactttc tgtgatcaaa ggagcaaaag ttttagaaaa gccaaagaat acacagtaat
     3781 aacggtcttt catagctttg gcaacctcaa tcattcgtgt tgtctcagct aaattgaata
     3841 ctgcaggagc aaaaagaatt gtcttcatga aatcctcctt attggaatga gtctatccct
     3901 tcgtttctta ctagtatact aaaaaacaca gcgttcgagt agcctatgta tattttgaat
     3961 attaaactgt ctctcgctgt tttcacaact tctaaattta aattttgtaa tcaagttgaa
     4021 actccaattt tcaaaaaaac acgcaacaat tttttgatcg atttatatgt tgtgccagat
     4081 aaatacgaga agttgtgtca ttaaaaataa acatgagaac ataaaaaaat
//
LOCUS       DACDSY010000184         4046 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDSY010000184 DACDSY010000000
VERSION     DACDSY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.187"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(29..607)
                     /locus_tag="IWK44_002679"
     CDS             complement(29..607)
                     /locus_tag="IWK44_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9551141.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(627..1295)
                     /locus_tag="IWK44_002680"
     CDS             complement(627..1295)
                     /locus_tag="IWK44_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9551142.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1310..2380)
                     /locus_tag="IWK44_002681"
     CDS             complement(1310..2380)
                     /locus_tag="IWK44_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9551143.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(2551..3915)
                     /locus_tag="IWK44_002682"
     CDS             complement(2551..3915)
                     /locus_tag="IWK44_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9551144.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
BASE COUNT         1143 a          840 c          654 g         1409 t
ORIGIN      
        1 taactatttt tctccctata taacagcttt acgcttctcc taatacacca ttcaaatata
       61 aatcgatcaa ttgcgccaca tttagcggtt cttccacagg tcttttcgac cgagtgatgt
      121 aaggtgctaa cactaagaaa aaatgccttg ctgcgatttc ttttgaaatc ccatttctta
      181 gcacagattg gtttgctgaa aagatttctt caaaaggttc acgataattt ttcttccata
      241 gaaagaaaag atgatctctt gtttcctttg aaagattgct ttcaagatcg tgaagcatca
      301 gaaacaaatc gaccagatgt gtctcaatca aaaactgcgc catttttgtc aaggttactt
      361 gaaatcccgt ttcgcttgtt tgaacgattt ctctcaaagc ttttccaaca tcttttaagt
      421 ggttttctaa aactgccgta tatagttttt ctttatcacc gaaataatga tagagattgg
      481 gttgggtgat cccaactttt tgtgcgatct ctcttgttga agtgccgaga tatcccttgg
      541 acataaacaa gtcagtcgct tctttcaaaa ttttttgctg tgtatctttg ttgtatcgat
      601 ggaccatttt tccacctgct ttttggttat tctttttctt tcaatacacc atcttccatc
      661 ataaatattc tatcgcattt gtcaatcagc cgtgtatcgt gagtaaccat gatcgttgct
      721 ttatttttgt cttttgattc ttttgccaag atatcgacta cttctcgtgc cttttctgaa
      781 tccaaactag cagttggttc atctgctaga ataatggacg gatcattgta caatgcacga
      841 gcgattgcga ccctttgccg ttctccacca gatagttctt ctggatactt gttgatcaac
      901 ttatccacgc caagctgctc aaataattct tttgcttctt tttgacgatg ttcatgtttg
      961 atcttatcta ctaataataa ctgctttttt actgtcagaa aaggaaccag attggaagct
     1021 tgcaaaataa agccaatttc tttaaaacgt agtttaacgc gttcactttc tttttgatta
     1081 ctgaattctt gatcattgat aaatacagag ccactgctag gcgtctgcaa tccgcctgcc
     1141 aaagtcaaaa aagtactttt acctgaacca gaaggaccga tgattgcaac aaattcccct
     1201 ttatcgatac tcatggttgt ttctttcaat gctttaacta cttggtcgcc atcttggaaa
     1261 cttttttcta cttctttaaa ttctattgct ttcatgctct tcctccttct tatccaattg
     1321 ccttcaacgg atcgattttt acgattgtcc gaacagaaaa tagtgcgcct aacacagcta
     1381 ctaccagcat caacaagcta atgacgccaa aaaacagcca attgctttgg aatggaaccg
     1441 catcaggtag aacaagggat gttcccactg ttcctaatag accaagaaga ataccaacaa
     1501 atgataaaat gaatgtttgt gccactacag aacgcgcaat aaatcctcct gtgatccctt
     1561 gtgctttcat tacaccaaag atattaatct tttgcatcgt cagtacataa atgaaaatcc
     1621 cgatcacaat agccgcaata acgatcaaaa atccgatcat aaatccaaaa gtcaatactt
     1681 gcgcattata tccaggtaat tcattgatga atttagaaat tttgatctgt tctaaatctt
     1741 caggtaaatt actgatttta cctctgacaa ctattgcgtt aatccgtgca ttttcacttg
     1801 tatcttcttt ttcgaagcgg atctcttggt aagcattgat ggttgtatag agaactggtg
     1861 agacattgaa tttggcatga tccgtaaagc cagttagttt aaacgtcttg tcactaccag
     1921 ataacttaac tgtatctcct acggctagac catattcttc tttcaaacta atatcgccta
     1981 ctgcctcatc atcgttatca aaagcttttc cttcaacaag attgggcatg atgaattgat
     2041 ttttatcgat accgaagaag ctaacattga tttttccttc ttctgtactg tctttactag
     2101 tcacgactcc aggtgtctgg gcaagatacg ccactttccc accttcgact tcttctgcta
     2161 tcttcttagt aatcatggac atccctagat ttgaatttgc atctttcgaa agcacaatcg
     2221 aatctgcctc ccacttgtcg actgctgttc gattatcttg agctaagcca taagctaacc
     2281 cagtcaaaaa aaataccagg taggcgatca aaaacatcac ccctgcaact aacgcataac
     2341 gtaactttga atgcataatt tcatttaatg ctaaaaacat ttaactcctc ctcctgaaat
     2401 aatcatttta tacaacaatt ttatcacttg ataaattttt ggcgaatatt atgctcaaaa
     2461 aattcttccg ctatttcatt atatgaattt tttattctcg attgattaat atttattata
     2521 tccccacaca ataaaagacc gtgatatttc tcacagtctt ttatctactc tattgctatt
     2581 tgctctctta gctagtactt taaaagctaa ctcattgagg tagtgaaaac gagaggtgtg
     2641 ttcagacaga aatccaaaat cctcaactgc tagctgtgga atcgtagttt tgtgtttcac
     2701 gatatccgca agcaggttca aatctgataa taagaattct tggcttatca gctgtccgcc
     2761 aattatttct aatgtatccg gatggtagat cagctttgca tttactgttt tgttttcttc
     2821 atcgtattgt ggaaatagat caaattctcc tgaacacgaa ccggtatttt gttccaagaa
     2881 tggcgcttca tcttctgtca gccctacagt tcctaaaaag tagtctataa tgactgctgc
     2941 tgttatcttt tgtctgaggt cgtaagcggt tggctgacca ctcaaagtta gcgctactgc
     3001 tcttgccata tgtacagctc gagtaactaa aggtaaaaag agatgccgtt tatttttgtc
     3061 aaaactgact gggacgatat ctccaatcgc atagatgtcc ggatcacttg tctgcagata
     3121 ttcattgact atcacagtct catcctcata caaagccact ttttctttta acaatgtcgt
     3181 attaggacga gtttgggcag aaaaaatgac catatctgcc tttattgctt gagatagaag
     3241 ttgtacggat actactttct cttctgattt tttccaatcg atcaagaatt catttggata
     3301 aaaattgatt tgcttagttt gcagctgatt ttgaaaactg ttgatcatat ccttatcgaa
     3361 atatcttgat aaaacattcg gcatataatc aactagatga ataatgtatc ctttatttct
     3421 caaagcatct gctaattcca atccaatata accagcaccg aagatcacta cttcttttgc
     3481 ttcttttaaa gcggcaagta cttgcttaga ttgcactaga tttttatagg tgtagacatt
     3541 ttcagcttca atttgtttat acaaggtgac aggagaggaa cctgttgcta cgattaattt
     3601 atcataggtt atggtctcgt tttttccttc tttggatgcg aggatgagct gatgtttgtc
     3661 tggatcaagt tcagttactc tccattcggt gatcagtttt gccccacttt cttgaagagt
     3721 ccgcatgtta tgggcaactt cagctaaact acgtatcttt ccttttaaat aaaggttgat
     3781 tccatttgaa acatagccta gtccatcttt atgatcttca tcgatcagta aaacttctgc
     3841 tttcggttga agttttctta gatttagggc agctgtgatg cctgcatggg aagcaccaat
     3901 gatcacgatt ttcatgtttt attcatcctt aaattcaatt tttctaaata acattctttt
     3961 aaaaattggt tcaaataggg gaaatcataa ttatacccgt ggtgctagtt aattttaata
     4021 tcctaaagaa tatctcaaag ttcctt
//
LOCUS       DACDSY010000185         3868 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDSY010000185 DACDSY010000000
VERSION     DACDSY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.188"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(465..1421)
                     /locus_tag="IWK44_002683"
     CDS             complement(465..1421)
                     /locus_tag="IWK44_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551145.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(1530..1775)
                     /locus_tag="IWK44_002684"
                     /pseudo
     CDS             complement(1530..1775)
                     /locus_tag="IWK44_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1920..2342)
                     /locus_tag="IWK44_002685"
     CDS             complement(1920..2342)
                     /locus_tag="IWK44_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551146.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2481..2911)
                     /locus_tag="IWK44_002686"
                     /pseudo
     CDS             complement(2481..2911)
                     /locus_tag="IWK44_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(3227..3490)
                     /locus_tag="IWK44_002687"
     CDS             complement(3227..3490)
                     /locus_tag="IWK44_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551147.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
BASE COUNT         1174 a          817 c          602 g         1275 t
ORIGIN      
        1 tgataaaaat attctatcgg ttctttttgc aaataaaaaa acatatagaa gctccattag
       61 attgaatcac cacaacacaa tcaaaggagc attccgtatg cttgattttc attctataaa
      121 actactttta ggaatcaaag aaaatcacaa cggttcaaac taaaagcaag aatatccgaa
      181 gtaccattgt ttcatcgatc atgagaaaag cattcacctc tgtctgcaga tgttcttata
      241 tcgctctgtc agtcctatta ataaagtact gttttgtttt caaaaaaact attacgagta
      301 ctttttaaga gaatgccttg caaatattca tcacacaccg ttatataagt caaatagttt
      361 ctttcagtcg tcaagaaaag attctcctgt ccttggcatt atcagttaag tagcaactta
      421 attctcggac ttgttctact ctagcaaaat ttattttcat caacttacgt ccaaatgttt
      481 ttttgaataa ttacttcatc gcgggtctgg aagacttgcc tcattcgata agtcactagc
      541 ttttggcgtc cttcaggatt tctagtatac accaactgac atttgggcag cgacgattga
      601 tttaattcgt ttagaaaaag gttagcgatt tgcttatttc ggccaaagcg ttctggtacc
      661 gtcagaacta tctttttccc ttcgattaaa taccgcggat tctccagcgg tgctaaaagc
      721 tccctcatag cttcagtaaa aagctgtcct tctcgatagc cagcattctc aagatagcat
      781 ctaaattcct gctgatcagc cgctacgatt acttttctat cactaacttc accaaggcgc
      841 tgcaacgtcg tttgaacgca tatggccaag ctttttttgc gggcacattc cttttcccat
      901 agcttttgtc gcctacgaat ttggaaatat tccagcaagt tacctttaaa tatagctgac
      961 cagaagctta gaggctctat attttttact actatcggca atgaagtttc ttgtttttgc
     1021 tttacctgat cgactttcct aacgacgatt ggcatagata ggtgccagcc atcagctaaa
     1081 gcagtactcc attgttcttt caaccgactt cgttcacaag aaaaatagaa catcgactga
     1141 gtataagttt ccatcgtggt ggtcgtatat tccagtggta aagcaaagcg atcgccacca
     1201 ctctcaattt ttggtgaaga ttcaaaggtc agacctgaaa tattgcccaa acgccgtagc
     1261 attcttttca aatcaggctg ttcagacaga ggaactcccg gtaatccaat cgctaaatgc
     1321 caaatgttcg ttactttatt ttgtgcacct aggcgtaagc cccgccctcg tagttgttgt
     1381 gtttgcacaa aagtggaagc ttgattagcc aagatcaaca gattaattgc aggacaatcc
     1441 cagccttctc ctagcaaact aacggttcct accaaaatat tcaacttctt ttcggttaat
     1501 aggcgagtaa ctgcttgcat caatgcttgg cggtttttgt catccaccgt caaataaaaa
     1561 taacccgaca tataaggatg ttcattcata cccgtcgcta gttctgccag ctcaacagtt
     1621 actagcattt tcggtaacag taagaactct ccacagatgg caccgatttg ctgagtgtta
     1681 cctaatagac gaacaacttc atcaaacaaa ggaaccacgc cataagctaa ggtaccatca
     1741 ctgcctgcta atacctcctt gcaaatacga tccatcaaaa tgaccgctgt cagttcttgc
     1801 ttcagctgaa cttcttcttt taacaaaatt tgacaaactg cttgcttttt ctcaggaatc
     1861 gcctgactac caacagtgtc tggatactct ggaaacaaat gcagcgactc atcaaacaat
     1921 catccagctt ttttcaattg ataaaaaata ggcaattccg gttgatcttc aaacatctgc
     1981 tgataaatag tgatcagctc ttttttacct aaagatggaa ctgaagcttt tttaccttgc
     2041 tgcttagaac tgcctaatag ctgccaatgt tcgggtatca attcatagtt atgatgattc
     2101 aagtaaatta atgcactagt gaacagctca aaattttcat aaatttcctt cgaatgctca
     2161 agcggatgct gaacaaattc taaagaaagc aaataatcca ccgtctcttg gctttccatt
     2221 aacgcttcca ccagctcatt ttgtttctct aaaaaggtaa aataatgctg agtagtttcc
     2281 tgattagccg gaatcaaata tagaaaatcc tgatgaggac atagaacctc ctcctttacc
     2341 aactcgggaa tgctaatttc ttcgtcaatc aggccatttt aactcaatat attgcttcca
     2401 ctccgtctga gagctatcaa tcggaggcgt ggctgtcaag gaaatcgtca acagattcgg
     2461 ttgttttttc ttcaagtcaa tcaacaattc accccagctt ttttttaaat gatgagcttc
     2521 atcaaaaaac taacagctcc agctgatcac cataagttgg ctcctccttc agtcggttgt
     2581 aaatggtctg atacgtttcc actgtaatat gactaggatg ttcaatcgcc ccagaaagat
     2641 agcaactatc tgctggattt actaagaaat attcctcgat tgttgttaac cactgtttct
     2701 tcaatattag ggaaggaacc aaaattaagg ttttacaatc caaacgtcgg atcatctcaa
     2761 ttcctaaaaa ggtcttgcca gcccctggtg gagccactag atggactgaa ttgttattta
     2821 aataagtagc aaagttagtc aatatacgct tctgatagct acgccactga attttttcat
     2881 cagatagcca catcccaggt ccccctctca cgtattttct ctatcataat ctaaatgaat
     2941 acagcaaaaa aaagatttct tcaagaatat gtgtttttat agtaatattt tacttactct
     3001 aaactcaaat gaaaaggtaa aaaaactggt agtacaatct tttaaattct cattgttttg
     3061 tacgttcttt actatcaaaa aaacaaaaaa aacgcgtttc tacgcgtgac caaaattttt
     3121 agctctataa tttctaagac tgatagaaaa tcattctgtt tttgaagtct tttttcataa
     3181 atactacttg atttattgtg tatattgtat aaaagattgt tcataattat tttttataat
     3241 taaaatttgg tattgatgac cagcgattcc gtatataatg cgcaatcaaa ttaagctttc
     3301 tgtctctggt aaatattcct gttctgtttt cttgtactcg taagatttct tcacttatcg
     3361 caaaattcat aaaattccac actttttcat aaacaaaatg tacacattta tctattactt
     3421 cataatagtc tacttgatat tcttctgtaa acatggttgc tactgtatct gcaccatatt
     3481 tgataaacat ttgggctact cccttaaatt gagaaaagcc cattagttta tctactaata
     3541 cttctaaaat ttcttgcttt cttctttaac atcgtcccgt aaggaatcat gtagtatgct
     3601 ggaggaagtt cattttctaa ataattgtat cgcttcaatt gctcaaaggc tattctctac
     3661 aattaattac accaattact ttatcttcac ttatataaga tttctaaaca tttttcgtca
     3721 aatgaagaga aaatattttt ttaaatgatc ttgctcaaga tttgtcaacg aatctttgaa
     3781 ttcgttttga agttctgagg aaggtatcat ttaaaaactc aatctgactt tcatcatttc
     3841 gtttactccc attttgatca ccttctac
//
LOCUS       DACDSY010000186         3834 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDSY010000186 DACDSY010000000
VERSION     DACDSY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.189"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            202..1077
                     /locus_tag="IWK44_002688"
     CDS             202..1077
                     /locus_tag="IWK44_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551148.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="IWK44_002689"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="IWK44_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="IWK44_002690"
     CDS             complement(1642..2973)
                     /locus_tag="IWK44_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9551149.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3826)
                     /locus_tag="IWK44_002691"
     CDS             complement(3122..3826)
                     /locus_tag="IWK44_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015075202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9551150.1"
                     /translation="MAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKLTVK
                     KGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTFSIE
                     LPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKSVYA
                     YITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEYSVS
                     RHLYQDFVFEAVFVEK"
BASE COUNT         1321 a          706 c          571 g         1236 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttca
//
LOCUS       DACDSY010000187         3828 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDSY010000187 DACDSY010000000
VERSION     DACDSY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.190"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            309..542
                     /locus_tag="IWK44_002692"
     CDS             309..542
                     /locus_tag="IWK44_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP9551151.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            539..946
                     /locus_tag="IWK44_002693"
     CDS             539..946
                     /locus_tag="IWK44_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9551152.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(2083..2601)
                     /locus_tag="IWK44_002694"
     CDS             complement(2083..2601)
                     /locus_tag="IWK44_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP9551153.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            3719..>3828
                     /locus_tag="IWK44_002695"
     CDS             3719..>3828
                     /locus_tag="IWK44_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9551154.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         1325 a          632 c          588 g         1283 t
ORIGIN      
        1 ggagaagagt atctctataa ttggactata ccaattatag aatgttttgt caattggtat
       61 gctctaaaat ttattaattt gtttcaaaaa aagcatataa acaggtatac gtttaataat
      121 ttaaaataaa tggtatacat aaatagggaa ggaaatagaa gggagcaatc taaatttttt
      181 gaattttctt aattaattat atagaaagaa caatctttct atagagttag acatgagagg
      241 gactatgctt gatttaaaat caacaagcgt atatactgta tatacaaaga aaaaggaggg
      301 agaagactat ggcaattaaa gaaagaaaat ttagggaagt ggggaactca gtagtattaa
      361 ctatttcaaa ggagtttctt aaagaaaatg ggttaaatcc cggagatacg gtttttttag
      421 atgaagaaaa acttcgagcg gcaattacaa aaaaacaagc cttatctgat gaaattgatt
      481 tagcaatatc tcaagcatta actgaatatg atactacttt tagagagctt gtcgataaat
      541 gagtattcat tacctagaag ccaaagatat tataaaaatg aatgtaatcc aaataaaaaa
      601 atactctcct aatgaacccc ttggtgtaaa ggatgcgaat gctttagaaa tgtgtgtagg
      661 gcaacttcga gcctcagctt ttggtgaaga ggtttatcct tcgatttatg aaaaagcagc
      721 tatcctactt atacaattga taaagaaaca tccttttcac aatgcaaata aacgtactgc
      781 tttcttagct acttttgtct ttttgaaaat caatggacag cttttagcta ttgatcaaaa
      841 agatgctgta aatttagcgg tatatattgc tacgtatgat aaggatttcg atcaactaaa
      901 atatgaagtg atcaacacta taaagacaca tacgcagccc atttaaggag ttactgtatg
      961 tgtctttata gggttattag ctgctatagc gaaataattg aagtaaatta caaatagcta
     1021 taattcaaat ccaactatat agaaaacgta aattttctat atagttggat taagataacg
     1081 actatttatt ttatcatatt tttactatac cctatacttt tacttggtat atttatgaat
     1141 aatcgcaaat ttacccctat actactagtt tgtaagttag attaataaaa atactaaagt
     1201 tatttgttct tttattaaat acacctacaa cttataaatt gatggtattt agataacacg
     1261 aaagaataaa aataacagct gatagtaaag tttgttgacg attgctactc aatcgaaagt
     1321 gagttagtgc gtatgaactt gcttctgaaa acttatgaaa tgaaagacct tttacatctt
     1381 gcagaaatcg gtactgggat taaactaagc aataaaaact aatgttcccc tcattctttg
     1441 aacttctact atttgattta tttattctga taaatgacga tttcaccttc aggatcttca
     1501 aatcccgtat agaatttatt taagatctct tcagtatcag taataggata ggcgataggc
     1561 cacataccct ttgatggtta gaataaactg ctcaaaatct gtatcgtatg ttggctttag
     1621 catattaaca aaggctatct ccaaattata aaccataagc gttgaatcgt tcttaacaat
     1681 ttcaattctt cctaacccaa agtcagaaga tataattacc ggacttgcct tacccttcat
     1741 gtgggttgtt gagattccat atttaaaggg aattgtcggt actatcggcg gtctgaaaaa
     1801 catcccaaaa ataaaaaaat ctttcccctg ctagtctcct aagtcccttt gtagcggata
     1861 tggcaccata atctcatttt atcgaagtga gtcatggttt atctgttggc caaccaaaat
     1921 tgatacaatc taacgatgca ccctctacgg cattctaggg gagtgcactt gcattcaccc
     1981 aataaaaacc ggcagttagc aaagaactta tgtagtcgta tgttcttact gttaacatac
     2041 aagaggtctc ctttccacaa ttgcttagca gtaaacggga atttacaaag ctaatctctt
     2101 ccattttctt atctttgttc taaaagtccc aaatggagca actgtattta catgaataaa
     2161 tttatatact tcccatacag ccgttttggt tgcctcgtct gcccattttc ttttgtgtgc
     2221 agtgaataga tcttcatcac tcattgaatc aatcatcatg ttaatatcat caatattacg
     2281 attgagaatt tcctctaatt ctttcaatgg tagatgtgca taggtatttg taaaccactt
     2341 atataactct cctaactgat tccatttaaa ttgatcagaa ggcgttttaa cagataatcc
     2401 atttctttca tcattttccc attttagaat taaattcgtc caaccaactt gatatgctaa
     2461 attttcagca ggtgttctat ccacttcctc aactctttta tccttcaatt cttcaggaat
     2521 agtttcaaat tctaaaatat atttagtata agtcctattt atttcttttt ttaattcttc
     2581 tttattttta tatgttctca ttatattacc tccataaatt cctaattgtc tcctttaatt
     2641 catctataat ttcatccacg atattttaat ccgttctaca atgcatatct gatatgcatt
     2701 gactgtctaa ctcatatgtg aatgatttat aaaatatccc aacgtctcat ctaaagaaat
     2761 gttgatcgtc agcaaggagc taaaaaatac gaatatattt cttttgtagt aaaatcggac
     2821 tggtttgcat atcattttct tcgtaaatat caaaatcatg ttaactacta tcaattacta
     2881 ataaaaacta ccactttaag cgttaagaac tgttaagttt agttaagtga ggatagttca
     2941 caaaatccat tgttaaactt cacgaaagtg gtaaaactca aaatgcttta tataggatta
     3001 cagcatcgct ctttcttcga ttgcgcgttg ggtaaaaaat attatgaagt aaaaattgac
     3061 gatgacacgt tccttacggc tcatcagatg aaacagctac aaaaacgaaa tgatcaactc
     3121 gaagaaaatt taattctaaa aaagcgattg ccatattcac gcctcgctcg acgaactatt
     3181 aaaggtcaag atttacctga agatcatcct ttttaggaaa ttgaagcttt ttatgttgca
     3241 caatgcattt ataatactac aatgatgttt tcaccagata ttcttctagg gtgcggtgta
     3301 atgaaacaga tacacttgca aaagaaaatt caccgcgaat ttactaaaat tatgaataat
     3361 tatgttaatc ttccgaaatt tttttccaaa attaagggat caagctggtg ttatagattg
     3421 tttaaggttg gcaaaaaaat aacttctttt taaacaatta tagaaaaccg atactctgtt
     3481 aagttggttg acacggtgtc gatttttatg actttcttat tctgaaactt cactaacgga
     3541 cagtacctaa aaagtctata gtatggacgt ttacgagtac tattttatca aaaatgctgc
     3601 tcatttcaac ttatatgttt tagtgaacta cccccactta gcttggatag gctacaatta
     3661 actagacaga aaaattaagg tgtgtagact agaagaaaac ataccaggag gaatttttat
     3721 gtctaagaga acacgaagaa ctttttcaca agaattcaag caacaaatcg tcaatcttta
     3781 cttagctgga aagccacgtg tagaaatcat tcgagaatat gaactaac
//
LOCUS       DACDSY010000188         3820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDSY010000188 DACDSY010000000
VERSION     DACDSY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.191"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            85..1053
                     /locus_tag="IWK44_002696"
     CDS             85..1053
                     /locus_tag="IWK44_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9551155.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1064..1879
                     /locus_tag="IWK44_002697"
     CDS             1064..1879
                     /locus_tag="IWK44_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP9551156.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            1947..2657
                     /locus_tag="IWK44_002698"
     CDS             1947..2657
                     /locus_tag="IWK44_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9551157.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            2693..>3820
                     /locus_tag="IWK44_002699"
     CDS             2693..>3820
                     /locus_tag="IWK44_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9551158.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1264 a          560 c          851 g         1145 t
ORIGIN      
        1 cgttatgtga tatctttaca tttaatcagt tacttttatt ttggataaaa ttaacatttt
       61 taaaatttta aggaggaaat gttgatggat gtagcaaaaa cggttaacgc aattgtagaa
      121 aaaaatgatg gagagatttc tggaatttat ttggtggctt gtggcggttc gctggtagat
      181 atgtatgtct cagattattt tcttaaagcc gaagcatcga agtgtattac aggcttatat
      241 acagcgaacg aatttaacca tgtaacccca aagcaattag atgataaatc tgttgtcatt
      301 ctatgttctc atggaggaaa tacagcagaa acggtaaaag caggagaagt cgctcaagct
      361 agaggagctg tgactgttgg tctgactcat aataaaactg cggatttatt aaaggtttct
      421 gatcatagtt tcctttacga atggggagat gatgcaaaaa tagtcaataa tcctatggcc
      481 atcattcttg atttaactgt atctttggtc aatgttaccg aaacatttga acattacggt
      541 aaattcagag aaggcatgaa ggttatcgat accgtaattg aaaatggcat taagcaggtg
      601 caggatcgta ttaaagtgtt tgctgataag tacaaagatg aaacaatgtt ttatatctta
      661 ggtagtggac catcatatgg acatgcttat ggtttttcaa tttgctcttt gatggaaatg
      721 caatggcttg attcaacttc tgttcattct ggagaattct tccatggacc ttttgaagta
      781 acagacaaag atacgaattt tattctcttg atgagcattg gacgcactcg tgctttagat
      841 gaacgggcaa aacgattcct tgataaatat gcagaaagag tagaaatcgt tgatgcaaag
      901 gaactaggat tggatatgat atcagatgaa gtttctgaat tcttcaatcc cattttattc
      961 tacagtattc tttcagaata tcgttcagct ttagcagatg tacgcaatca tgatttagat
     1021 gttcgtcgct atatggggaa agtggagtac taggaggatt aaaatgaagg tattaggctt
     1081 aggtgataac gtcgtcgata aatatgaaaa tctgggaatt atgtatcctg gaggaaattc
     1141 attgaatttt gcagtttttg caaaaaaact gggacatgaa agtagcttta tgggagtttt
     1201 tggctcagat gaggaggcag ctcatgtttt atcagcaata aaagaaatag gactggacaa
     1261 cagtcactct cgctttgaaa aaggcgagaa tggctgtgcc agagtgaaga ttgatgaggg
     1321 cgaccggatt ttccttggca gcaattcggg aggtgttact cgtgaatacc cgattcagct
     1381 tactgaagaa gacagagaat atttaaacca gtttgaattg attcatatgg gattatatag
     1441 ccatgtcaat catctattag aagagttaca aaatgtttct ggaaagattt cttatgattt
     1501 ttctgacgac tttacggaag aagaggttca gcgggcaatt gataaagttg attttggctt
     1561 cttttctata gaagactttt ccgatgaaga agtaaagcag tttttgatga agcatttttg
     1621 tgagcgaaat gatgtattga ttgcaacccg aggagaaaaa acggctattg gttatgatgg
     1681 agcaaagttt tatgaagtga accccgtgct agaagaacca gttgatacaa tggcagcggg
     1741 agattctttc ttaacagctt tcctacttta ttttctcgaa ggggacgata tagtcaccgc
     1801 gatgaaaaag gggaatgaat ttgctgggaa gtcttgtctg gttgacggtt cgttcggctt
     1861 tggtattcat tacgattgag cttgttttaa attcctgcat ttgataacct aaagaaagaa
     1921 atcccggtaa agtggaggtt aaaaaaatgc tggatacaaa tgcacaatta ccattgtact
     1981 tacaattaaa agaaatatta aaaaataaaa tcaatacagg ggagtatccc gagaatacga
     2041 aaattcccgc tgaatcagag ctgatagaaa gttatgaggt cagccgaatc acagtgagaa
     2101 aagcagtaga agaactggta gaagaagggt acctacttaa aaaacaaggg aaaggaactt
     2161 ttgtcaaccg gcataaagtt tttcgtaaaa ttgaatatat tatgggtttc agtgagagtt
     2221 gtaaatctaa tggttttaca ccgactagca ttgttttgga acgcaagatc attccagcaa
     2281 ctgaagggat ggcaaaaaaa ctcgatatcg ctcaaggtga tgatgtttta tatatccaga
     2341 gaaagcgaat ggcggataac atccccatta tgttagaaaa caattatttt tcttatgaaa
     2401 gatatagttt cttaatggag gaagagctga ctgattcttt gtatgaagta cttaaagtcc
     2461 aaggtacctt agccatcaat cctggggaaa ctgcacttga aattgtcaca gctgatgacc
     2521 aaattgccaa aataatggaa gtagctgttg ggacgccctt tttctttatg gatactttaa
     2581 tcaacgatca aaacaacata ccaattcatg taggtaatca atattacctg ggagaagtat
     2641 atcgttttta cttatgattt cctgtttgga tttcaaggag tagattttat ttatgaagca
     2701 aatattgcgg tcaaaaatga tttttgatgg agagcagcca cctttcaaag gttatattgt
     2761 aatcaatgaa gggatcattg aaaaggtgga aaacaactgg aattacagtg atctattaaa
     2821 tgaaaacacg gaattaattg cttatgatga tcaattcgtt atgccagcaa ttcatgataa
     2881 tcatgtattt ttcagtggtt atttggctat gaatgctggc atagatttaa gcgatacatc
     2941 ttcagctaac gaggctgtag agcgtattaa agctgtaatg aaccataaaa aagcagatga
     3001 accaatttat gctcatggct gggatgctgc cctgtggaaa actgacccat cagaagagct
     3061 attgaatgaa ataaaccact ttggtccaat cacagcgatc gacaaaaatc gcagccattg
     3121 ttggatgaat gctattgcta agcagactta tggttttaca gaaaaagaaa ccagtgctga
     3181 aagtcgagta ttattaatta aggaaatgct agcaaacaaa aagcttttag cagaggttta
     3241 cgctgaattt gaagcattac tgctttcaaa aggtgttata tcaatgaaag aaatcgtttt
     3301 tgatgacgca gaatttcttt ctaatatacc tcagaggaaa atacttagta acctatatgt
     3361 gcaggcagtc caagaacctc tacagcatga acttctatat gagtataagg aaaaagtttt
     3421 ccctgaaaag gtccgctttg gtggagtgaa gatcatggta gatggtgttg ttgcggatag
     3481 aagtggagat atttacggtg tttatcagtc aggagtagct tcacccgaga ttgactatga
     3541 ggcaattaaa gcagaagttg ctgcattcaa tgaagtagag attccttgct gtttgacaac
     3601 ggaaggagat aaagcgggtt ataaagctgc gtcaattctt gtcgaatttg gaaagcggtt
     3661 acctgaaggc gtttttaatt cgattagtga tttggaaatg gttactcaaa agacagcaga
     3721 aaaaatgaac gaaggacaag tagtagcaga ggtatatcca caaattctcg gcttgaatca
     3781 aacgttagaa gaggcttaca tgtcaactgt tataaaaaat
//
LOCUS       DACDSY010000189         3805 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDSY010000189 DACDSY010000000
VERSION     DACDSY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.192"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..253)
                     /locus_tag="IWK44_002700"
     CDS             complement(<1..253)
                     /locus_tag="IWK44_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9551159.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEI"
     gene            complement(255..1694)
                     /locus_tag="IWK44_002701"
     CDS             complement(255..1694)
                     /locus_tag="IWK44_002701"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP9551160.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            1879..>3805
                     /locus_tag="IWK44_002702"
     CDS             1879..>3805
                     /locus_tag="IWK44_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP9551161.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGT"
BASE COUNT         1194 a          669 c          641 g         1301 t
ORIGIN      
        1 ctatttcgag atactcaatg agttcagagt aaaagatatt actaatatta ggaaaaataa
       61 gaccaataag ctgaggtgac tttccttgaa gtccacgtgc taaattattt ggtttataac
      121 caagagtttt catagcttca tgaacttttt gttttgtttt ttctgataaa tatcccttat
      181 tattaatgac gcgtgaaacg gttgttgggc taactccagc taattcagcg acatcagtta
      241 attttgctac cattttaata ttttaattca aagtattttc cagtaggttc gcctgatttg
      301 atttgaatac cattttggtg attttcaggg aatacacgac tagtaaacac tttttctcct
      361 ttattaataa aaatctcaat gattgagtta tctacaaaaa tgttagcagt tgtttcttta
      421 gtatcaattg agcattcacg aatcgtacca aagtcagtgg catattgaat gcctgctttg
      481 ctgcgatcta aaataatttt tccttctttg gtatctacaa tcaagctaag tccatttcct
      541 ttactatcag agaaaagaag taactccgat ttctgatttg gaggaaaagt tagttcaagc
      601 tcataagtat tatttgtttc tgcttttgct gtaaattgtt cagaactagc acgtaatgat
      661 gtaatagctt ctactggata ttggtaaagt ttaccatctt ttattgaaag ttcttttaca
      721 agactcattg ctccctgata atcatactta tcagttggat aatcgatatc aggtagtcca
      781 atccaactga caatcagagc acgaccatca ggagtattga atccttgagt cgcatacgct
      841 tcaaatccat aatcaagatt ttgaatttct gaagcgccca caagttttac tttttctatg
      901 tcaagcgatt gacagatctt aaaagtattc ggatagatat tcccataatt tagctcagat
      961 ttatccaacc cttgaggaca ataaatgaga acaggttttt tgtctacaaa tactagattt
     1021 ggacactcaa tcatgtattc tgaaccagtg ccgccaaaat caaggttgcc aacttcctcc
     1081 catgtttcca cgttattgtc atccgctttg taaagtttga taaagcctga tttttctaaa
     1141 ttttgtgctc caacaatcgc atacaattgt ccttcataat taaacatttg tggatcacga
     1201 aaatgatcag ttacatcagc aggtttatga ataagaacat tctcaccttt cgtaatgttg
     1261 tagtctttat ccatccatgc tccgatttga agtgtatcac gttcccaatt ttcatttcga
     1321 acgtttcctg tatagagtaa gaatagattc ccctctattt catatgcaga accggaataa
     1381 gcgccgtggc tatctcgttt tgtgtctgga agaagttctg ctcctgttac atgaaaatga
     1441 actaaatcat ctgattcatt gtgtacccat tctttcaacc catgagcagc tccaaatggc
     1501 caattttgat aaaaaagcgt atattttccg ttaaaaaaag aaaaaccatt aggatcgttt
     1561 aacaatcctg ttttaggttc gatatgatag ctagcacgcc aaggtgatcg ttttacatta
     1621 tccttaattt tatccatctc atctttagtc caatcagaat aagcacgata tcgtatttct
     1681 cgaggtaagt tcatatttta ctccttcaat ttatcttttt acaactagta tggtaaacgt
     1741 tttccgttga aaaatcaatc atttcattta aaaaaataaa ctatatgaca aacgtttgac
     1801 atatttttaa gatatgctaa aataaatttg catttcagtg aaccgttttc acgtaaaaat
     1861 aaaaaaggag tgattcgaat gaattacagt aaagtagctt ccgacgtaat acaagcggtc
     1921 ggaaaagata accttattgc agctgcacac tgcgctacac gtcttcgtct cgtactaaaa
     1981 gatgacacga aagtgaatca aaaagcttta gacgaaaatc cagatgtgaa aagcacattt
     2041 aaaattaatg gacaatatca agtgattatc ggtgcaggtg atgttaattt cgtttatgac
     2101 gaacttatca aaaaaactgg actttcggaa atttcaacag atgatttaaa acaaattgct
     2161 agtaaagata aaaaattcaa ccccattatg gcattaatta aattattatc agatattttt
     2221 gttccaatta tacctgccct tgttgcaggt ggtttattaa tggcattaca aaacttttta
     2281 acagctgctg gactttttgg accaaaatca attgaagaaa tgtatcctgc gattgaaggt
     2341 atttcctcaa tgatccaact gatgtccgct gcaccattca tgttcttacc aattttagta
     2401 ggtatttcag ctgctaaacg ttttggagca aaccaatttc ttggtgctgc aattggtatg
     2461 atcatgacaa caccagatct tggtggctca actgaatatt ggaatatttt tggttatcat
     2521 gtttcacaaa caaactatgc ttatcaagtt attccagttt tagcagctgt ttggatatta
     2581 tctatattag aaaaattctt tcataaaaaa ttgccatcat ctattgactt tacattcacg
     2641 ccattattat cagtaatgat tactggattt ttgactttca cagttattgg ccctgtaatg
     2701 ttattgttgt ctaatggaat tacagacatt attgtttggc tatataacac tactggattt
     2761 attggaatgg gtatttttgg aggaacatat tcacttatag tcatgactgg tttacatcaa
     2821 tcatttccag ctattgaaac tcaattactt tcagcgtgga caaatggtat cggacatgga
     2881 gactttatct ttgtagttgc atctatggca aacgttgctc aaggtgctgc aacatttgcc
     2941 atttggttct taactaaaaa ttctaagaca aaaagtcttg cttcctctgc gggtgtctca
     3001 gcacttcttg gaattacaga gcctgccttg ttcggagtta acttaaaata ccgctttcca
     3061 ttcttttgtg ctttaattgg ttcaggaatt gctacagcag tagcaggatt acttaaagta
     3121 gttgctgttt cacttggttc agcaggtttc ttgggatttc tttcaattaa tgcaacatct
     3181 attccatttt acgtattgtg tgaattgatt agctttgtag ttgcttttgc aattacttac
     3241 ttctacggta aaacaaaagc tgctggtatc tttaaagcag aagaaattgc tgaacaaaca
     3301 ccagttaatt cttcaaaagt agcaacaaca gatccaatat ctactgatgt tgaatcaaca
     3361 caaacaaaag tttcatcaga aacaattgtt agtcccattt ctggtaaagt gattagtctt
     3421 tcttcagtaa atgatccagt cttttcatca gaagctatgg gaaaagggat tgccattaaa
     3481 ccaaatggaa acacggtcca ttctccagta gatggtacta ttcaactcac gtttgaaact
     3541 ggacatgctt atggtattaa atcaaataat ggtgctgaag ttctaattca tattggtatt
     3601 gatactgtat caatgggagg aaaaggattt acacaaaaag ttactactaa tcaaaaagtt
     3661 agaaaaggcg atattcttgg agcatttgac cgttcaatga ttgagaaaga aggtcttgat
     3721 gatacaacaa tgattattgt gaccaatact tcggattatt cagaagtaaa tatagtagct
     3781 gaaaatacag tcactgaggg tactg
//
LOCUS       DACDSY010000190         3800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDSY010000190 DACDSY010000000
VERSION     DACDSY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.193"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(171..911)
                     /gene="truA"
                     /locus_tag="IWK44_002703"
     CDS             complement(171..911)
                     /gene="truA"
                     /locus_tag="IWK44_002703"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9551162.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1102..1737)
                     /locus_tag="IWK44_002704"
     CDS             complement(1102..1737)
                     /locus_tag="IWK44_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9551163.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(1884..3125)
                     /gene="sstT"
                     /locus_tag="IWK44_002705"
     CDS             complement(1884..3125)
                     /gene="sstT"
                     /locus_tag="IWK44_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP9551164.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3323..>3800
                     /locus_tag="IWK44_002706"
     CDS             3323..>3800
                     /locus_tag="IWK44_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551165.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSK"
BASE COUNT         1257 a          782 c          585 g         1176 t
ORIGIN      
        1 gaagagaagt acaacattct cactagattt ttatttaaga aagacttttt agctgtacac
       61 ataaacaggc taaaaagtct ttttttatat ttctttaatt tgcttcatac attatttaac
      121 atcgatattt atactgtgaa acatgcgttt catccattat caaagtattt ttatcgataa
      181 taaacttcat ccaaaaacag accttgtgca gggacggttt ctcctgcatg ttgccgcact
      241 ttgctttcaa atatttcatc gattgtttct atcggcatgg cacctgtgcc gatttctagt
      301 aatgtcccca tgataatccg caccatttta tataagaatc cgtctccgac aaaagtaaaa
      361 tgaagcattt ctccctttcg ttcgatagat aattcatcaa tcgtacgcac tgtggatttt
      421 tttgattttt tcaatgcaga aaaaccaatg aaatcatgtg tacccactaa tttttcgcaa
      481 gctgcattca gcttgtccat atccaactct tgagggaaat aaaagctgtg gtttcgctca
      541 aatgctgaag gaatcacgtt gttccaaaca taatagctgt attttttgcc caccgcatta
      601 tatcttgaat ggaagcgctc aggcatttct tcgattcttt tgatgacaat atcccgcgga
      661 aggtaacgat tcataaagtc gatcatttct gccagtccaa tttctgtgtt tgttttaaaa
      721 tttgctactt gtcctcgcgc atgggtgcca gcatctgttc ttcctgatcc aataatttct
      781 atctcttcat ttgtcatctg atgcaatact ttttcgattt ttccttgaat ggttttatcg
      841 tcatctccaa gtctttgcca tcctgaatat ctttttccgt catattcgat tgtcaacttg
      901 atgtttctca tattttagtg gctcctctgt cttccatttg gtcttctctc taccttttta
      961 agattgtagc acaaaatata gctccttgct tctctaagac agaagctata caagaaaact
     1021 gtggaaatat atatttcaga aaaacaaaaa acagccaccg gatgaagtat ccgctgactg
     1081 ttcaatttga cggctcaagt tttactgttt gagatgcaag atactcaacc tggcaatggg
     1141 cacataatta aagaaaaaac tgattctttg gcaaaaacct attttattca gtaatggcca
     1201 agaatttata gaaggaattc ttgccagtcc ataaatcgcc gcaaaaagca aactgatcac
     1261 agacagtcca aggtataagt ggcttacgtc catttgccca ttttttcgat agatcaagaa
     1321 aagaagaact ggaaacaata aaaaaccact gtaacctaaa cctgatccga tattatggac
     1381 ccatgtcgag acattccacg aagattcatg ggtattgata ctgaacaaac ctgtaaagat
     1441 acaatcacct ataccataga acgcgatggc tgctgtaact agggtagcta acacacgaga
     1501 tgtactaaca aatgcagcat acaatgcagg caatgacagg atgaaaaaga tccctgaaac
     1561 cacagaccaa ataagaaacg cttttcttac aggactggaa acctctccaa atacactgat
     1621 cacgctcttc atttgatcta attcaggata atacaatcct aatacgtacg gagtcaaaaa
     1681 atcactcagc acgcctaata ataaaaagaa aaaaccaaat ttacgtaaaa aatccatgat
     1741 acgcctccaa taacaattac ctatgctttt aattgttatt ataaacgact ttatcttgat
     1801 ttaacagaaa cctctattta cacgaacatc tatataactc attaaaaact cgaatcattt
     1861 taaaaatact aaatatctaa cgttcaacgt tcacttaatc catctaaaca atctttcaat
     1921 ttgatttttt ccccacttct tttacgcgcg cctagctctc ctattgcagt aaacaacaga
     1981 tcacttgacg aatttaaggc agtttccatt gaatcctgga taacaccgat aataaatcca
     2041 ataccgacga tttgcatcgc gacatcatta gagataccaa acaaactaca tgctaatgga
     2101 atcaataaca gtgaaccgcc cgcaacacct gaagctccac aagcagctaa agcagacaaa
     2161 atacataaaa ggactttcaa aacaaaaggt gcttcaatcc ctaaagaatg tacagcagtc
     2221 aacgtcatca aggaaatggt aatcgcggca ccgcccatat tgatcgtagc acctaacggg
     2281 atcgatatgg aataagactc ttttgtcaat ttcaatcttt cagccaacat catgtttatc
     2341 gggatatttg cggcagaact tcgggtaaag aaagcgggaa gtgcgctttc tttcaaacaa
     2401 aagaaaatca atggataggg attttcttta ataaacaaaa acgtgaggaa tgggtacaca
     2461 actaatgcga caaacagcat cgtaccgatc aaaacaagta aaagctgcag atactctgcc
     2521 aatccagcga ttccagtcgt agcaattgat tgataaacca atcctaaaat ccctatcgga
     2581 gcaaatgcaa taatcacttg tacgacttgt gacaacgcta tagatatctg atccaccaac
     2641 tcttttgtcg attctgaacg tgctcgaaaa gctagtccca acaatacagc ccagaataag
     2701 acgccaaggt aattcgcatc aataagagat tgaacaggat tttgtgtgac attcgtcaac
     2761 atagtagata aaacactttc caaactttgc ggtgcttttt cagatactgt ttcttgtaaa
     2821 gtaataggaa cagtaaaaat tgaacttaca ataactgcta caagtgcaga aagcaatgtt
     2881 cccactaaat ataagatcaa aataggcttc acgaatgtct tattcccaat tttatgcttg
     2941 gcaattgacg ccatcgtcaa gaaaaaaacc agaataggtg cgattgcctt caagcaactg
     3001 acaaacaacg ttcccaatat tgaaataaat gtccatgtgg ggaaaaagat accaaacaat
     3061 attccgacta aaataccaac aacaatacgt tgaactaacg acatatccaa aaacttctct
     3121 aacaaatgac caccctttca caattttaac aatcattgta tcattttatg caaagaaact
     3181 aaaggtataa ttttctaaaa attctaacaa tacaaacaac catttcttgc taattttgta
     3241 ctttttctgt ctacacgttg ttttttgaga tattctaagt taggatgtta ttaatgggca
     3301 acttatcagg aaacgttttc ttatgttttt gtccaatttt tttgtgaggt gtttattctt
     3361 gaaaaaaata aagaaggaga gttatggctt cctgacaatc tttgtcgctt gtataatgat
     3421 ggttttacag cgtatattga tttcagcagt cactattcct acatatgcaa caaaccctgg
     3481 tcgattctac atctatacat ttttacaaac ttccgttgtt attggtgcaa atttgatccc
     3541 actttacatg ggttaccaat atcaaaaaat cgcccgttta aaagttttac attatttaag
     3601 tcgttttgcc tttatattcc taatagccac actagtttgc aatatctttt tttacgtaca
     3661 agctggcagc ttgaatattc gtgattattg gcttatccta acacctatca gtcagaatta
     3721 ctttacttat gctgtttctt gtactttgct tttttgtagt attccttata cagttggatt
     3781 tttaaataaa ttatcgaaaa
//
LOCUS       DACDSY010000191         3713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDSY010000191 DACDSY010000000
VERSION     DACDSY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.194"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IWK44_002707"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IWK44_002707"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP9551166.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="IWK44_002708"
     CDS             complement(2261..2512)
                     /locus_tag="IWK44_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP9551167.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="IWK44_002709"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="IWK44_002709"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP9551168.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="IWK44_002710"
     CDS             3462..3680
                     /locus_tag="IWK44_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551169.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          797 c          688 g         1203 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aag
//
LOCUS       DACDSY010000192         3675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDSY010000192 DACDSY010000000
VERSION     DACDSY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.195"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            7..570
                     /locus_tag="IWK44_002711"
     CDS             7..570
                     /locus_tag="IWK44_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551170.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(631..996)
                     /locus_tag="IWK44_002712"
     CDS             complement(631..996)
                     /locus_tag="IWK44_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551171.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1072..1317)
                     /locus_tag="IWK44_002713"
     CDS             complement(1072..1317)
                     /locus_tag="IWK44_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9551172.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1387..1641)
                     /locus_tag="IWK44_002714"
     CDS             complement(1387..1641)
                     /locus_tag="IWK44_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551173.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1805..2128)
                     /locus_tag="IWK44_002715"
     CDS             complement(1805..2128)
                     /locus_tag="IWK44_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551174.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2315..2388
                     /locus_tag="IWK44_002716"
     tRNA            2315..2388
                     /locus_tag="IWK44_002716"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2349..2351,aa:Ile,seq:cat)
     gene            complement(2773..3270)
                     /locus_tag="IWK44_002717"
     CDS             complement(2773..3270)
                     /locus_tag="IWK44_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9551175.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3310..>3675)
                     /locus_tag="IWK44_002718"
     CDS             complement(3310..>3675)
                     /locus_tag="IWK44_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HAP9551176.1"
                     /translation="DPSLSYLKLALTDKSEEKLQKLQRDLQADGIQSVFSDPHFLEIT
                     PTGITKAHSLVFLADYLGIESSDIMAFGDQENDIAMLNYSGLSIAMGNAQRQVKEIAN
                     QVTLTNEEAGVAYILKNFY"
BASE COUNT         1268 a          630 c          650 g         1127 t
ORIGIN      
        1 ataactgtgt tatatattaa aaaaggcata atagttataa gcttttttaa agaatattta
       61 gaaattatta attttcgtct gaaaacaaaa aaagaaagcg ttattatttt agtctatttt
      121 caggggggga accttatgat aaaaaaacta tttttactat caatattaac gctagcaatg
      181 gcaggttgtt ctgactctgg cttagcaact gaactgatca caaaatcaac tgaagccacg
      241 acagaggagt taaaatgtgg aaacgatcac agtgactaca gaggcttaaa tcgacgatgc
      301 atgggaatat taggcagaag caattgtaag catcgatgta gtaatcgaag aaggtgtaga
      361 agaagcaaca gaaaaatcaa aagaaagatc aaaagaagca atctgtgctg tgcatcgtat
      421 acagacgtcg aagatgcggt acttttaaac cacgtcagga gatgttcatt tatgtctagg
      481 gaagttggcg atgaaccaaa actagagtac gcttttagtt ttgcacgtga ggatgaagat
      541 aaccctaaaa cttacgacat tttagcttga tctaagatgg ttaaagcttt ttaatgaagt
      601 aagaggagat agaaggcttt tctagttata ttaggtaaga taaattcctt tttgctccaa
      661 atttgctaaa cattctgcta gataaacttc atcgtgttcc ggataaatcg gcacgttgcg
      721 ctctacagta ggtaaatcaa aaaatgcttc gcatgatttg ccacgataca taggattttt
      781 ccactctggt gctgggctgt aaccacgttt ttccatttca tccatgacaa gctgatgata
      841 ttggaataat ctataaggag aatagtgaaa aacataatcc acagtagcat gtttctttcc
      901 ccagccattt cctcttaatg cacagcattc ccgatgttgt cctaaaagct gttgacgagg
      961 aagctttgaa atcaattttt catgccataa tcgcatagaa atgtctcctt tatatataga
     1021 ttgttaacct tagacgttaa atgaaaaaac cagcaccaag gctggaaaaa atcacttcag
     1081 tttgcttgct gtttctgagc gaccaattag gtaatcaacg gaaatatcgt aaaagtcagc
     1141 gatatagatt aattcaatga tgcttggaat ttcttctcct tcttcgtact tacgatattt
     1201 gaataaagga atacttaacg cgctagaaac agctacttga gataaagatc ggccagttct
     1261 taagaaattc agacgacaac gtaaagtatc cagtaaaata gcttcttcga tactcattta
     1321 atcacctttc attatagtag aatagttata atgactatag aaagctacac atgcaatcaa
     1381 tagtactcaa tgttttttat ttaaacgtat caaataaaga atgacgatca ataaagacaa
     1441 aaccagtcct gataattctt ctataaaaac actatcgggt tcaaagtagt tgtaattaaa
     1501 aactttccac ataaagtaga tagccggaag agtcaaaata gctaaataaa accgttcttt
     1561 gttccaattc ataagataaa caatacttgc gaccagtgcg accgcaggca cccaaaacaa
     1621 aactctcacc aacgtaatca tgtaacggac tcctcaacga ttcatataat aaaagataac
     1681 atgttattgc atacatcaca aatgaaatgc taggaaaata aaagaagaat agatcaatga
     1741 aaaatgaaaa agcagaacag ttttctgctg ataagcagga actgttccgc tgaatgtgtc
     1801 ttatctatcg agaaaaaatc aatggattga tatctaagta attcaataca tataaaaaga
     1861 ggacgatcgc attcactaaa aaggaaagaa tgatgatccc atagcgaatg gaaacatatt
     1921 taacaaatac ttttgtttga atacgggcat atctttttcc tttatagctg agcatcatga
     1981 caagaagcaa aagggtaatc acaccttgaa aaatcattcc aataaacaga attcctgagg
     2041 tttgataatt taattggaga aaataagctg ctgccgcaac taaatcgaat aaaatcgcaa
     2101 taaatggcaa aataatcact cctaacattg agaataaata aaagaataac attttttcta
     2161 aaataagcaa ataaaaatta aagtaacaaa atagttatga cacaagtaaa agaaataaga
     2221 ataacaatat tattttactt atattttgaa acttgtgaga gtttgtcatt cgtgctatag
     2281 ttgaaaagta acaaaaataa attttattta tgatgtacct ttagctcagt tggttagagc
     2341 agacggctca taaccgtccg gtcgtaggtt cgagtcctac aaggtacata agaggttgtg
     2401 aaaaagctgc cctgcatcaa gtaataagaa cagccaagta aaaacagctt cttatgtttt
     2461 tacttggctg ttcttattac tgcagatgca agcttttgaa taccgtttaa ggaagaggtt
     2521 gtgaaatcag cgttttgacc tgagtattag cttaacaaac ggaaaaatag ttcctcatat
     2581 tttgccgttt gttaagctaa taccgagggt cagctgattg aacaccattt aagaaagagg
     2641 tcgtgagcct gccgtttcac tctgtgtcat aaaaaagcca atctactttg atagaaaaag
     2701 cacgtaggac taaaacagtt ctgcgtgctt tttttattgt gtgttaaata tcgtaaatga
     2761 agatagatat ttttatgatt cgacgataga gacgttgaaa tctttcaaca gctctaaagc
     2821 ggattctccg tgttttctac tggagactaa cattggatca acaaaaaagt ctgcttcagg
     2881 aaaagcactc tgcaggccta tcagattaga gagtacacac aagtttgttt caatcccgac
     2941 tacttcgatc tctttttgtt cttctttgct ttcaaataaa gtttcctgga tttcagctaa
     3001 agtttcaggt ggaatcgtaa agtaatactt tttaatcact cgttcatttt cttgtggaga
     3061 aagaagaggg atcaattgca aatcttctcg ttcttcttct ttgtccttca gttcaactgg
     3121 tatatcccgt gtgaagagaa tatattcatt attttggcgt gctttagcta atctttgagc
     3181 aatacgttct gccaattgat ctgactctgg aatataaaat tcgctgtttg gatcaagaat
     3241 atgattttgc atatcaataa taactaacat tttctttcct cctacattca tatcgtcatt
     3301 ttattctttt taatagaagt tttttaaaat ataagcaaca ccagcttctt cattcgtcag
     3361 tgttacttgg tttgcaattt cttttacttg tcgctgtgca ttccccatcg caatactcaa
     3421 cccgctgtaa ttcaacatag caatgtcgtt ttcttgatcg ccaaaagcca tgatgtcact
     3481 cgattcgatt ccaaggtaat ccgccagaaa aacaagtgaa tgagctttag tgatacctgt
     3541 aggcgtgatt tctaagaaat gcggatcaga gaatactgat tggataccat ctgcttgcag
     3601 atctctttgt agcttctgta gtttttcttc actcttatct gttaatgcta atttaagata
     3661 gctaagagaa ggatc
//
LOCUS       DACDSY010000193         3675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDSY010000193 DACDSY010000000
VERSION     DACDSY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.196"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWK44_002719"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWK44_002719"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP9551177.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IWK44_002720"
     CDS             complement(1172..1867)
                     /locus_tag="IWK44_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP9551178.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IWK44_002721"
     CDS             complement(1880..2596)
                     /locus_tag="IWK44_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP9551179.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IWK44_002722"
     CDS             2926..3534
                     /locus_tag="IWK44_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP9551180.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
BASE COUNT         1050 a          756 c          660 g         1209 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagct
//
LOCUS       DACDSY010000194         3664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDSY010000194 DACDSY010000000
VERSION     DACDSY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.197"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            75..470
                     /locus_tag="IWK44_002723"
                     /pseudo
     CDS             75..470
                     /locus_tag="IWK44_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            790..1104
                     /locus_tag="IWK44_002724"
     CDS             790..1104
                     /locus_tag="IWK44_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9551181.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="IWK44_002725"
     CDS             1208..2386
                     /locus_tag="IWK44_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9551182.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            2789..3082
                     /locus_tag="IWK44_002726"
     CDS             2789..3082
                     /locus_tag="IWK44_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP9551183.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1255 a          537 c          715 g         1157 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatcccgtcc
     3661 taac
//
LOCUS       DACDSY010000195         3661 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDSY010000195 DACDSY010000000
VERSION     DACDSY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.198"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            412..1452
                     /locus_tag="IWK44_002727"
     CDS             412..1452
                     /locus_tag="IWK44_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP9551184.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2276..2608
                     /locus_tag="IWK44_002728"
     CDS             2276..2608
                     /locus_tag="IWK44_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9551185.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3227..3568
                     /locus_tag="IWK44_002729"
     CDS             3227..3568
                     /locus_tag="IWK44_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9551186.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1353 a          482 c          676 g         1150 t
ORIGIN      
        1 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
       61 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
      121 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
      181 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
      241 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
      301 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
      361 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
      421 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
      481 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
      541 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
      601 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
      661 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
      721 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
      781 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
      841 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
      901 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
      961 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     1021 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     1081 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     1141 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     1201 gaaagtggta tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     1261 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     1321 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     1381 aaagtatcaa gtgaaaaacg tttgaattat gaaagctccc caagcactat tacgaaagac
     1441 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     1501 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     1561 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     1621 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     1681 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     1741 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     1801 ttaattgtct gctatgcttg tgatgaattg attttcgttc tgcacttttg gatgataaat
     1861 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     1921 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     1981 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     2041 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcacaatgtt
     2101 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
     2161 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     2221 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     2281 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     2341 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     2401 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     2461 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     2521 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     2581 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     2641 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     2701 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     2761 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     2821 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     2881 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     2941 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     3001 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     3061 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     3121 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattga
     3181 tataaaacat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     3241 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     3301 aggatttgtg acgtttagaa gaggcggaaa ttgtaaagga ttagatgttt tagcattaga
     3361 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     3421 tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     3481 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     3541 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     3601 ttagaaggag tcacgatgat taataaaaag gtagtattac cttaggcatt gagtaaggaa
     3661 t
//
LOCUS       DACDSY010000196         3644 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDSY010000196 DACDSY010000000
VERSION     DACDSY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.199"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..892)
                     /locus_tag="IWK44_002730"
     CDS             complement(<1..892)
                     /locus_tag="IWK44_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9551187.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDS"
     gene            complement(1349..1777)
                     /locus_tag="IWK44_002731"
     CDS             complement(1349..1777)
                     /locus_tag="IWK44_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551188.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(1805..2443)
                     /locus_tag="IWK44_002732"
     CDS             complement(1805..2443)
                     /locus_tag="IWK44_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9551189.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2447..3217)
                     /locus_tag="IWK44_002733"
     CDS             complement(2447..3217)
                     /locus_tag="IWK44_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP9551190.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
BASE COUNT         1085 a          695 c          547 g         1317 t
ORIGIN      
        1 tagaatctga agtgaatgta ctcaaaacat tttcaaagtc tttgtctttg atttttacat
       61 tagcgtcttt caatacttcg ccaattactt tattctggaa ggtactgtca cttaattttg
      121 tgtctgttgc aatttctttc aattcttttt tgtatttatc catgtcgttg cctttgtttt
      181 ggtttttcac cattttaacc acataatatt ccgtagtata agttgaagca ttggtagaag
      241 tgatcacatc agaaacttga ccatctttca acttaaaggc tgcttctttg acttctgccg
      301 gaaccgttgt agtagtggaa tcaaatttca cttttccgcc atcttctttt gtcgtagtat
      361 cagttgactt gtctttggct aatttagaga aatcgtctcc cttatcagca gctttcttca
      421 catcttttgc atcatcttca cttgctactt ggataatttg agcttccact tctggatgga
      481 aagattccca agccgttttt aaatcttcat cggtcaattt gatatgtttc tttagacctt
      541 cttgaaaggc tagactttgt ttgatactgt ctttgaatgt ttgttctgtg taacctgcag
      601 atttcaattg tgaatcaaat gaatcaccca attgtttctt tgtttgatca tattgagaat
      661 cgatttcttt ggttgatacg tcatctccat atttatcttc aaatacttga taaacgatca
      721 actgttgcac agcggaatct gctccttgca tttttttcaa gctatccatt acttcttgtt
      781 cgctgattgt accgccttta taactaacgg ctgttttttc accggcattc gtacagcctg
      841 acaataagcc agtgcctagt acgatggcaa gtgctaaaaa tctttttttc atttatcaat
      901 tctcctactt ttcgtttttt gcgaattgct aaaaacaaag gtgttcaaga tgaaaatcat
      961 caagaacacc tttactaatc tgctcgatac gtcctgaata gatataaggc tacacaatcc
     1021 agaaagttcg ctcagcagaa taaatgtcaa gtagcacatc acaccttaat atactacttc
     1081 ttttgttacc gtttttcaag tctatcttgc gttccgtatt taacgtaact aattcttaga
     1141 acaaatgtta tcgattttgt ttttcttctt ccgctgctgg gatattcaaa tcagacactt
     1201 ttatgttcga ttttccagac atttttttgt tgtttttatt atgatatatt tttatacaac
     1261 taaaaatgaa tatattaaaa ctaccaaaaa taacttttaa cgctgtttca ttatatgttt
     1321 tttctatttt atataaactt ttggatactt aataccaacc ggttggattg taccaacatg
     1381 agaatgtgtc agttctattt cctgtcgcac ttgcataggc ccatactcct tttgttgcat
     1441 ttgctgatcg aatagagttt ccaactttcg cttttgcacc gtgacgatta tagacacgac
     1501 tatagtggtt agaatgacct gtttttctcg tacaagttgt atcactataa taaacccatc
     1561 ttccagagct atgattaatt gctgttctag aaatttcccg gtctactggt aaatttgact
     1621 taataatatt acctttagca tcaaaatgaa tctctcctgg ctttaaagat ttgtaataga
     1681 tttcaccgtg aactgaattt gcttccactt cagatgacat agcgtttcct ccaacaaaac
     1741 ctatccctag tgcacaaata ataactgctt ttttcataaa acgacatcct ttcctttttg
     1801 atttttaaag catgatcttc ttgtttccta ttttggtcag atatggatca tgcgtgacta
     1861 cgataactaa cgtaccttgg gaagcttgtt gcgataagat ttcaactact ttatccttat
     1921 ttcttttatc caatgaccca gtcggttcat cggctaaaat gattttgcta ttcttcaata
     1981 agactctcgc acaggcgatt ctttgcttct caccaccaga taatgtgtag ataggcgtac
     2041 ttagcttctt gtttatattt acttttttca atgcctcata ctttgctgtt cgcttatctg
     2101 aaacatttaa attcgaaaac ttcaatccaa tatctaaatt aaaatcgatt gtttcatatt
     2161 caatcaaccc aaaatcttga aaaagaaaag atatggtatt tctttttatc aagcgcttct
     2221 ctctcccagt taattgtttc gtctcttgat cgaacaaata gaattgtcct gaactgaact
     2281 gatcattcaa tcctaatata ttcaataacg tacttttacc tgctccactt tctccaacaa
     2341 cagtcacaag atccccagca tccgcagtga aacgatccaa atgaagaatc gttttgtttc
     2401 gaatgatttt tttcgcacct tttatcacta tcaacggctc caattgttaa cctccattca
     2461 tcagcaataa ctgacttttc ttttctgtag acaaaataaa aacaactgaa agaataaaat
     2521 caatgaagct aaagaaaaaa atattaaaaa gatatttagg ctctctttct ataagaaaaa
     2581 ggaagattac acttgtcgtt ccccatttga tgaggttcat catgaagaaa tctttatacg
     2641 tttcaaagag cgaataacca cttgaacgaa agatagcgat tttccgacaa ttgtagacaa
     2701 aatatatgca taacatgaca tagctcaaca agaagaaaga aaagaaagtg agtaaaaata
     2761 tctgtatcaa gtaatgaaat gatctgctta acactttgac agctatctct tcagtctgag
     2821 tatacggaaa tgataactgc aagttatcac gtaactgact ttcttcaatc agctcaaata
     2881 acctttcttt cgacaaagag cctatcttga tctttaacgg tggatagatt tctccgctga
     2941 gaatatttct ttccaaatac atactgtttt tcaatgtcag tatttcaaca accggataat
     3001 gttcgatcca ggggatagaa ggaataaatg tataaatggg ttgattgttt tttataaata
     3061 gaaatttcgg ttctttttca ctgatttctt gataatattc tgttatttgc gccaggtcat
     3121 tttttaaatc tgtatgtcta tatcgttcag ggatcaatat aacagggtgc gtttcttttt
     3181 cggttatttg gatcaacctt ccagagacat caatcaaagg aaatttctta agataattca
     3241 gatttatttt tatccctctg ccaaactgtt cattttcttc attcaagtaa tctgatacat
     3301 tgactaatat acttccgttt ttattcaaag tttcatataa ttcctgatct gctgctgatg
     3361 caaaacgatt ggaataaata aaatccacgt ggtttttccc gatggttact ggataaaata
     3421 catgatagtc ctcaccagcg atttcttttg gtggttcatg ggaaacagct aactgtgaag
     3481 aagaaaaaac gatagttgct aattcgacat tggcaacggt caaaagaaga gaaaacaaca
     3541 atccaaatgt aaaaagaatg tattggatat agttgaacgc ttctcttaac gaagcgattt
     3601 tttcaactaa taaaagagaa atcaaagaga taccatatat tccg
//
LOCUS       DACDSY010000197         3491 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDSY010000197 DACDSY010000000
VERSION     DACDSY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.200"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            50..1789
                     /gene="yfcC"
                     /locus_tag="IWK44_002734"
     CDS             50..1789
                     /gene="yfcC"
                     /locus_tag="IWK44_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9551191.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1946..3130
                     /gene="iadA"
                     /locus_tag="IWK44_002735"
     CDS             1946..3130
                     /gene="iadA"
                     /locus_tag="IWK44_002735"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP9551192.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3241..3444
                     /locus_tag="IWK44_002736"
     CDS             3241..3444
                     /locus_tag="IWK44_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9551193.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT         1111 a          590 c          815 g          975 t
ORIGIN      
        1 ccatttttta ttcatcaaaa ttactgaaaa aagaaaggaa gaaataaatt tgaagaaaac
       61 aaattcttca ggaaaaccga aacgcgactt cactaaatta tcgacacccc atacgtatgt
      121 cattattttt ggtgtagtca tctttgcatg gatactgact tttgtcgttc cagcaggaaa
      181 gttcagcacg caagatattg aatacaaaga tgcaaatggc gaaacaagta ctcgtactgt
      241 tttacgtcaa gattccttcc gttatgctta cgagctggat aagtcgtatg tgtttgatca
      301 attagaagaa ctgcaagatc atccagcaga acgtgaaaaa ctggatgttc cggaaaaagg
      361 attggaaaaa gtcattgctg acggagaaaa aaatcttacg caggaaaaac tagatgaaat
      421 ctccttgaca gatgatgtgc tttacgatca gtacggcgaa aatatctatg atacttctaa
      481 aaaattgcat aaaacagcca aaatatgggg aacagatgat tttggcggct ttggtttctt
      541 aaattttgtt tttgaaggac tagtatctgg agataagtat ggatctgctg tagggatcgc
      601 tgcattgatc cttgttgttg gaggtgcttt tggtattatt atgcgtaccg gagcgatcga
      661 cgcaggtatc tatgcgttta tcagtaaaac taagggactg gagcgtttag ctttaccgct
      721 attattcttt gctttttcat tcggtggcgc tacattcggg atggcagaag aggtaattcc
      781 attctcaatg gtgatggttc catttgtcat tgcgttagga tatgactcga tcgtagcagt
      841 aaccgtgact tatgtcgcat ctcaagtcgg aaatgcgacc tcttggatga gcccgtttag
      901 cgtagcggtg gctcaaggga ttgctggaat ccccgtgttg tctggtgcga cattccgatt
      961 gattatgtgg gtagttgtta ctgcattagc cgctggttat atgatgatat atgcagaaaa
     1021 aatccgtaaa aaaccagaaa gttcgttgac ttacaaatca gatgattatt tccgttctca
     1081 tatcaaaaag acatctgatg aaaataaaaa attcatgctt gggcataaat tgatcttatt
     1141 agagatgtta gttgtccttg tctggatcgt ttggggcgtg actcaaaaag gctattacat
     1201 tccagaaatc gcttctcaat tctttgtgat gggattagtt gctggaatca ttgctgttct
     1261 tttcaaatta gatggaatga aagtcaatga catcgcttct tctttccaat ctggtgcagc
     1321 tgatttagct ggtacagcaa tcgtagtagg tatggccaaa gggattcttc ttgtcttggg
     1381 aggatctgat gcatctgttc cttcagcttt gaatacgatt ttgcatggga tcgggacggc
     1441 gttgaccggt gttccagctg tcattggtgc ttgggcgatg tacatcttcc aaagtttgtt
     1501 caacttggtt gttacatcaa attcaggaca agcagctttg actatgccaa tcatggctcc
     1561 actggcagat ttagtaggtg tatccagaca agtagcagtt cttgcttatc agcttggttc
     1621 aggttttatg gacgctttta cacccgtatc agccagcttg attggtgtgc taggtgttgc
     1681 acggatcgaa tgggcaaaat gggcaagatt ccaaatcaag atgcaaggat tcctgtttgt
     1741 tttaggaact attttcatca tgattgccat tgcaataggt ctgcagtagt actggagacc
     1801 tgtaaataaa aaagtaatcc aaacagtaga taaggactcc aagaaccgtc attttacggt
     1861 aaaatcaaag gagtaaagat cctatcgttt ggattatttt ttaagatcct atcatcagtt
     1921 atacttaaag aaagaggaaa aatgtatgaa aatcattcga caaattgaag tgtttgcacc
     1981 agattactta ggaaaaatgg atgtactgat tgccggtggg aagattttgg ctgtagaaga
     2041 acagctagaa ggcggctacg aaggagtgga agtagaagaa ctttcaggtg agggaaaagt
     2101 actaaccccg ggtttcattg actgtcattt ccatatttta ggtggaggag gagaaggcgg
     2161 ttatcaaaat cgaacgccag aagtcacatt gagccaattg actacagctg gtgtaacgac
     2221 agttgtcggt tgtttgggaa cagacggaga aggtagagat atgactgcgt tgatcagcaa
     2281 agcgaaaggt ctggaagcag aaggaatctc cacttatgta tacgaaggtt cgtaccgatt
     2341 gccggtaaaa acagtaacag attctatcat caaagacttt ttgacaattg ataaaattat
     2401 cggaatcgga gaaatcgcag tatccgatca ccgttcttct cagccaagtt ttgaagagtt
     2461 cgctcgtgca gtagcagatg ctcgtgtagg aggcatgtta tccggaaaag ctgggatcat
     2521 caatgtccac ttaggcggag gaaaaagaaa gctagagcta ttgacacgaa tcgtagaaga
     2581 aacagaaatt ccgatcacac aatttttgcc tactcatgca aatcgcacac cagaattatt
     2641 tgaagcttgc gtggcatttg ccaaacgtgg tggcacgatt gacttcactg ctagcgaaga
     2701 tcctgatttt tgggagaaga cagatggtga agttcgcttc agcaaagcac tgaaacgatt
     2761 gatcgaggaa gatgtttctt tagataattt cacgatgacc tcagatggac aaggaagctt
     2821 gccttatttt gatgaaaaca atcatttcct agggttagga gtaggaagtg ccaaggcatt
     2881 gctagtaggg atcaaagaag ctgtgcaaaa agaaagtatc cctttggaaa tcgccttacg
     2941 agctataacc agtaatccag cacgtatatt gaaattggat aaaaaaggaa aaatcgaaat
     3001 tggtgctgat gcagatctgt gtatcttgga taaagaaacg cttgatattg atacagttat
     3061 cgctaaagga gaaataatgg tccaagaaaa agaagtcaaa gtatggggga ctttcgaaaa
     3121 aagtttttaa tcaacagctc taactatctt ttttagaaaa cgagcgttct gtttgtctgt
     3181 atctgttgtt gatgttaccc tgaaggtaga tcaataaaca aactaagagg aggaagcaca
     3241 atggcagatt taaaaggacg ttctaaagat atgaaagata aagtagtcgg tgaaggaaaa
     3301 gaagcatacg gcaaagcaac agacgacaag agcaaagaaa cagaagggaa agcacaatca
     3361 ctaggtgctg acatcaaaga aaaagctcga aatttaggcg acgaagtcaa agacaagttt
     3421 tctcgaaaag acgaaaaaaa ataaaaacca cttattacta attgaatcaa gcaaataaaa
     3481 aatccgaacc a
//
LOCUS       DACDSY010000198         3436 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDSY010000198 DACDSY010000000
VERSION     DACDSY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.201"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(474..1037)
                     /locus_tag="IWK44_002737"
     CDS             complement(474..1037)
                     /locus_tag="IWK44_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9551194.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(1052..2095)
                     /locus_tag="IWK44_002738"
     CDS             complement(1052..2095)
                     /locus_tag="IWK44_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551195.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(2228..2632)
                     /locus_tag="IWK44_002739"
     CDS             complement(2228..2632)
                     /locus_tag="IWK44_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9551196.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(2865..>3436)
                     /locus_tag="IWK44_002740"
     CDS             complement(2865..>3436)
                     /locus_tag="IWK44_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9551197.1"
                     /translation="PYILGLFYPKLNQMTTVISVFGDVDSPVRRAFLVWSVVSGLFFV
                     LSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFTGLFSINTNESSCNLSTWIHNIG
                     SGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTVISLLIAGLYGLARIPSISQFAF
                     FKQLGFFQRLSFFFNYLPMICLSFFQLRK"
BASE COUNT         1053 a          706 c          585 g         1092 t
ORIGIN      
        1 tcacttaaaa aagcactaaa attataagag agggaggtaa tggtctgaac accaagaatt
       61 gttccaacat cgaattgctt aactaatgag ttcaaagctt aattatgtgg ctcttatatt
      121 taccgagcgt ggattgcgat ctctttagcg aaacaaacat ctttcataaa aaaaacagcc
      181 atatccgctt tcttggatat gactatttac tactacttta ctatctttca ttcttcttgt
      241 ctcacttatg ataaggcgct gcgtatcatg agtgaatgat attaggaaaa cttcataaaa
      301 taaatctttt atacatttcc acttctagat ggtcattcat gttagaagaa tcaaactatt
      361 tttttactgt cgtaataata ttaaaacact agaacaaatc aattaaattc cataaaatat
      421 aaataataga cctagcatca tctggtataa aatggatgct aggtccgtta tatttactca
      481 aaattgattc tacgaataga tcttcttatc atttctattt tagtagtcat gcgttgttgt
      541 tcttctaaag gtggtaatgg tataagtagt ttagataaat ttgtaccatt tacatttggt
      601 tgcccagttc cgtaagaaat tttttctaat aacttccaat ataatggcga gtctaagaag
      661 cagtctacgt attctgataa taatttctca actaacctaa ttcgaatcaa gtaagaagca
      721 aaaacagatt cctcacttat ttccttaacc aagaaacttt ttcccatagt tcctcctgtc
      781 cttgctatca aaatgtcatt ttcttccaat cttaaatcaa ctagtttaga attactaata
      841 tcacagtaag gcacactgga ccaatttaca cgtccctctt gtatatctgt gattctcaaa
      901 aacttaacat tgccttgttt tttcgccgaa tctgtatagc catattgaat cttagtagaa
      961 atacttccta aataaaccca tagtcgaaca tcgctagatg atttttcttg tcgttcttta
     1021 tgaacaacag tgatcatctt gagttgtccg actatttttt agttgcagca atctttttgg
     1081 gacgcttatt tgcatgaagc aacgttgcta agtctagtaa taaaacattg gctaacacag
     1141 gattggcttt ctcgcgatac gctgcggcgc cactttcaaa caatactgct agacgatctt
     1201 ctacaacaac gatttgttcc aaatacggtg gaaatttgat tcgttttagg atccggctag
     1261 gagagtcccg ctcggacact aagagttccg aggtataagg accataagaa cgactggcta
     1321 agagataatg gccataaaaa gccacacctt gaatcttatc agatgttttt ctgctcattg
     1381 tcacggttgg ttggattttg tctttgcggc gaattgctgt tttcatttca gctatcgtcg
     1441 gcaatagata gaaagtcaac tgtccttcac cttttagtgt gaagttgcca gataccagtt
     1501 gatcattgtt ttgagtcaaa tacgatgctt gtggaatctc tgagagatca gttgtcagcc
     1561 aataagcgat tggctgtccc gtctcttcag atgtatctgc taagatatcg atcaaacgta
     1621 aagcggctac tcgtccagta gcagatcctg tgattgtcaa ccataaaagt tcccgttttg
     1681 tatcatatac taagccccct acatgtggcc gtttaggtaa aatcagcagt ttgatcggct
     1741 gtcctgtttc tctatccagt acatgaataa tagaatgatg ctgatggtca tagcagtagg
     1801 cagaaataaa cacaaaatct tgagaaaaag ccaacccttg cggcgtcatc cattgacaaa
     1861 cctcattttc gccggtttcc ggcaccacca ttcttgtttc tgttaatcca ggaatgttat
     1921 attgttttgc ttcagccttt tcccaagctg cttgaacaat aggataccgt tttaactttt
     1981 tttcaatatc ctgatgtatc cgttgctgct tccaatgctt aaactttagg acatagtaaa
     2041 caagaaggcc aatgaccagc aacactaaaa tcattattgc tacccgctgt gtcatttttt
     2101 tgcctccttg ttctattcga tcaatcgtcc atataccgct attaattacc gtaactacat
     2161 gtttctttta ctagcgtttc tttataggat ctttcataat ctcatttata gtaacattaa
     2221 gaatccctta ttctactata gtcacttcca catcaatatt gcctcttgtt gctttagagt
     2281 atgggcaaaa tgcgtgggct tcttctgcga ttttctctgc ttggtccttc tcaagtcctg
     2341 gcaaagaaac ttctaaaacg accccgattt ggtagccccc atcttctaat tgaaataaag
     2401 atacccgtgc tttgacttca gaatcagact caagattatt ttcttccagc atgtgttgaa
     2461 ccgcgccgtt gaagcaagat gcgtatccag ccgcaaaaag ttgttccgga tttgtccctt
     2521 cactatggat tcctgggcgg tcgatcttca tgtgcatact tccgtttggt gcctcaactt
     2581 cgccatcacg gcctccacga ttgatcattg ctgtttcgta catttttttc attgagatca
     2641 tcctttcttt cgtcgttcta tattaagtgt aacgagtatt ttttaatctg acaaaggtaa
     2701 tgcttgcatt cggcgacatc agttagacaa catcgatgat cagtgataga agcagtttat
     2761 ctatacaaaa aaagaagggt tctatgatat ttggtgtcct tactcgcctt gagtaacaac
     2821 accaaatatc atagaaccac ttaatcagct tcacactgct ttttctactt tcgtagttga
     2881 aaaaaactta aacaaatcat cggcaaatag ttaaagaaaa aacttaatcg ttgaaagaag
     2941 cccaattgct taaagaaggc aaactgacta atcgacggaa tccgtgctaa tccgtaaaga
     3001 cctgcaatta gtaaactaat aactgttaaa acaagataaa aatggctaag cgtaccttga
     3061 ccactttgac gataaagcag aacaagtaac aatgggaaaa gtagaaagcc tgcatagcct
     3121 agcccagaac ctatattatg gatccaagtc gatagattac aagagctttc attcgtattg
     3181 atactgaata agccagtaaa aatacaatca cctatcccat ataaaccaac tgttgcaaca
     3241 acaagtattg caagtgtttt tgatgttcca acaaataggt gatacaacgc aggcaacgaa
     3301 agaacaaaaa aaagtccaga tacaactgac cagaccaaga aagcacgtct gactggacta
     3361 tctacgtcac caaacacact aattacggta gtcatttgat tcagtttggg ataaaaaagg
     3421 ccaaggatat acggtg
//
LOCUS       DACDSY010000199         3405 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDSY010000199 DACDSY010000000
VERSION     DACDSY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.202"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..621
                     /locus_tag="IWK44_002741"
                     /pseudo
     CDS             1..621
                     /locus_tag="IWK44_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1069..1515
                     /locus_tag="IWK44_002742"
     CDS             1069..1515
                     /locus_tag="IWK44_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551198.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1517..1654
                     /locus_tag="IWK44_002743"
     CDS             1517..1654
                     /locus_tag="IWK44_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9551199.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            1691..1984
                     /locus_tag="IWK44_002744"
     CDS             1691..1984
                     /locus_tag="IWK44_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551200.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1981..2205
                     /locus_tag="IWK44_002745"
     CDS             1981..2205
                     /locus_tag="IWK44_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9551201.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2202..3221
                     /locus_tag="IWK44_002746"
     CDS             2202..3221
                     /locus_tag="IWK44_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9551202.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT         1185 a          603 c          707 g          910 t
ORIGIN      
        1 ttactagatg acccttactg ggtaggtaaa acatcgttgg gtgagaatat tgcaaataaa
       61 ggtacgacgt tcccaattaa atctagcgcg tacgaaatat ataaaggtaa cacggaagaa
      121 gagcttataa taggtcaaac gtatactatc acgcttaaag gaacaaaacc cgcaagtcaa
      181 acctttgtag catataatta ttggaacgtt aattttggag aactaaaacc agttgaggga
      241 ttgacagacg tatggtctct aacattcaca ccaacgaaac ttgaaccggg tttgcctaaa
      301 gaccttcgca tttttcagtt gcctaaagaa acggccggcg catgccaaat tgactggctc
      361 aaaattgaaa aaggcgacac ccgaaccccg aatattagtc agtttaaata ctttggtgaa
      421 ggattgaaag atagcaacaa tccgaacgac tacagctggg acatcacgcc tgaatatact
      481 gaaaaaggct tgaatgatac ggttagtttg actgaaccgc aatccatcga aggattgaaa
      541 aatttcgaag atggtattca atcaaaagga aaatctgtat tgacatcaga cgacaacaaa
      601 tatgaagtcg taaccttaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
      661 gaaggaaaaa ccgtcactat ttattttttt gcgttgaatg ggaaaagcag tggtggaaat
      721 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
      781 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
      841 gccattaaat ggcgaagaaa ctcaagttat ggatcagctt attcatttgt tatcacttat
      901 tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc cttacggatt
      961 cgagcaaatc attgtgaatg acgaagaaca tttaccggaa gaatgcacag aagtcgaacc
     1021 accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat gaaaaacatt
     1081 tggaaatatg gccgtactgg cggagagtat gcaggaaaag tattggacga catgcttgta
     1141 tccgttcctt acacagatca gccaccgctc gaaggggttc gttcagatgg cgaaccgcta
     1201 acgattgctg atcagatgtt tgatcctaaa ttgaaccaat ggattatttt agcgaacgcg
     1261 ttagatcaca acgatttaaa caatctcaaa gcgatgtacg aggctcttga acatgaaaac
     1321 ggcaacctaa aacagctaaa tgccaaactc atgctaagcg atgtagcgat taaacaggaa
     1381 aatactgcat tgaaagaaaa agctgacagt ttagcacaaa tcaattcaaa aatgatgctt
     1441 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccagcttca
     1501 aaaggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
     1561 ttttactgac gatcaagttc gactattcgt tccactatgc attacagacg aagaagcaga
     1621 taaaatcatt aataaagata agagcgcatc ttaattgatg cgtttttatt taaggtaaag
     1681 gagttgtcac atgattaatt taggggaatg gggaatgata gcaggatcaa taaccgctat
     1741 cgtttctttg attttattag taattagacc gattgctgca tctttctcga aaattactga
     1801 gactctttca aaagtaagcc acaatttaga tttgctgact aaagatttag aatcgagcaa
     1861 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
     1921 tacagaaaaa ttggtggaac acacgcaaca aatcaaaact ttgtttaggg agagaagaaa
     1981 atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta
     2041 gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt
     2101 gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc
     2161 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta
     2221 ttactgcgat gagcctgtta acggctcttt ttttattgcc aattaacgga tttgcctata
     2281 ctatcaacaa tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc
     2341 agtatatttt actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt
     2401 atatgcaacg ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg
     2461 tttaccaagt cggacaacct ggttatgtac agtacggtgc tggttcatat gctaatgcca
     2521 atagtcctgt gcagattgag ttacaacaca cacatgataa agcaactttt gaaaaaaact
     2581 ataaagcata cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacat
     2641 tagacacgcc ttataaccaa ccaggaatca aatcacattt atgggtaact caaaacattt
     2701 ggggcgatca cactgatccg tatggatact tatcagaaat gggtgtaagt aaggaaaaac
     2761 tggcttacga tttagctcat ggatttacag acgaaaaccc aacgacttct gagaacaagc
     2821 ctgtcattga tccaacccga gcaggtgcag caaatccaac tttatcagat ggaacgaacc
     2881 attctcacat tgatcagttt ggagaaatcg aaaatgcgaa cttacacgtc gctggatggc
     2941 acatcgctaa ctataagtac gagtatattt tcattatgga ctacaatact gggaaagaat
     3001 tagctcgagt aagagctgat ggaatttata gaccggatgt aaatcaagct tataatactt
     3061 ctggaaatgt tggctatcat gtatctttta acatgcgtaa cttccctaat aagaaagtct
     3121 atgtcatgat gcgggcaacg aatgatccag agggaaacac taaaggcggt gcgcaagatt
     3181 tccatgacaa acgttggtat ttaaatattc ctaaacgata aaaatagctc ctcgttgagg
     3241 agcattacat aactatattg acaactataa aaatcattcg ataaaatagt gatgttatcg
     3301 catatcttca ctatcaccca taatagtcac actccaagct atgcgataac aggtttgttg
     3361 ccacacattc tactggttga ttgtttatgg ctttatgtgg caaca
//
LOCUS       DACDSY010000200         3400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDSY010000200 DACDSY010000000
VERSION     DACDSY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.203"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..391
                     /locus_tag="IWK44_002747"
     CDS             <1..391
                     /locus_tag="IWK44_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551203.1"
                     /translation="LNLDSVNSNAQIKDQRVAEHNNKKMSLTKNIYSMSDEELYKEFG
                     DLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNHRQFIDVNTDSNAIS
                     IKDLKELLNSKRNELKQIKETNSVELE"
     gene            405..1094
                     /locus_tag="IWK44_002748"
     CDS             405..1094
                     /locus_tag="IWK44_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551204.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1219..>3400
                     /locus_tag="IWK44_002749"
     CDS             1219..>3400
                     /locus_tag="IWK44_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP9551205.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDET"
BASE COUNT         1150 a          455 c          720 g         1075 t
ORIGIN      
        1 tttgaattta gattctgtta attcaaacgc tcaaataaaa gatcaaagag tagctgaaca
       61 taataataaa aaaatgagtt tgaccaaaaa tatctattct atgtcagatg aagaattgta
      121 taaagaattt ggtgatttat ccggggaaca gttagaagaa agtattccag gaattagtga
      181 ctcagataaa attgttgtct tgtctgctgg tgggcaatta atgggagatg gcaaatccga
      241 attagttagt tataaacaaa atcacagaca gtttattgat gttaatactg attccaacgc
      301 tatttcgatt aaggatctaa aagagctttt aaatagtaaa agaaatgaat taaagcagat
      361 aaaagaaact aattcagtgg aattagagta aaggaagatt cattatgaaa aagatttttg
      421 gtgtttctgt tgggctactt atgatgttaa catttggaaa tgcagtatat gctgctgaga
      481 aagaagttat ttcagatcca gagcagtacg cctcacgata ctttgctgat tttgaagaag
      541 aagataaagt acttgcattg ccagaaggtg gttttttaaa aggggaagct aaaattgtat
      601 ctatggataa tccagatata attcaaggtt catataattc agaaactgat cctgcttcag
      661 tttcagtgac agaagcaaaa aaaattgttg ctgaaaaatt caaaaatgat attgaaacta
      721 ctccaattat gcctcgagga gcatttccac ccgatgttgg tactagcgga caccttcttg
      781 tactagcagg aggaggaagc tataaatcac agccatttag tggtgcggga tggcgttttg
      841 gaggtatggg tgtaaaacca gctgatggta caggcaactt tttacgttgg aaatcctaca
      901 ttgacgatgg aagagtagga aatgtaaatc aagccaacgc aactaaatct ggttctattc
      961 aaggaatggc attgtatgct cgtgaaggat tcaaatactt tgcaccaaac gggcctggtt
     1021 ggacaatgaa tataggtatt tattacactt ataatcctgt tgcaggtacg tattatgttg
     1081 ttcaaaatat ttaataaaac agcacaaaga gatttctttg tgctgtttta ttatttttag
     1141 ttgttgacaa acactaaaat ataaaatata atcaacacat aaacgaacgt atgttcggca
     1201 agaaagcagg ggatttttat ggatgagatg gttttaagta ctcagaaatg gctaaacaag
     1261 aaatacagta atgttacagg gtttgataag gttccagaaa atgggcgaac agggtggcct
     1321 acaatctatg gacttattga aggactacaa gtagaacttg gtattactaa tctagtagct
     1381 aattttggac ctaccactga aaaaatgtac gataatcagg ttactcctaa atggggcaaa
     1441 aatctaccta aaaatattgt atttcttatt caaggtgcct tttggtgtaa ggggattaat
     1501 ccaggtggct tcgatggggt atatactcca tatttagata ctgctgtaaa agagttgcag
     1561 actgatgcag ggtttaatgg aagcgctgta actggagttt tggatgcgaa atgggccaaa
     1621 gcgttatttg atatgagtgc ttttgttctt gttcccggcg gagatagtaa aattagaaaa
     1681 atgcagcaat ggttaaatgt aaattattta gaatacacgg gaataatgcc ttgtgatggt
     1741 atttatcaaa gaaattcaaa tcaagcatta atttttgcat tacaagctga actaggatat
     1801 tctcccagtg aagcaacagg gtcttatggt aatgggacaa ctagtaaaac ttatcctgtg
     1861 tctgaaggta actcgggaaa ctatgtaaga ataattcaat atggattata tgttaatggt
     1921 tattttgaag acggtacatt cgatggtatt ttcactaaat atatgggttt agaagtactg
     1981 gcattccgaa aatttatggt attgcctgaa tatactgaaa tagcagatgt tgttgtaata
     2041 aaaggactat tgtctagcgc tggagatatt agaagatctg cagatggcgc agatacttct
     2101 acgcaattaa ctagatctca aatccaaaca ttggttgata atgatataaa aatagttggt
     2161 agatatctta ctggtactgt tggaattgga aaagatgaac gaaataagta tctaactaca
     2221 gaggaattga ataatatttt ttctaaaaat ttatctgtgt ttcctatcta tcaagatgga
     2281 ggtgcagcat tagcttattt tacttatgat cgtggcttat cagatggtaa gaaggctata
     2341 gatgcagcaa aaaatctaaa tataccatta acaacagtta tttattttgc tgttgatcta
     2401 gacatgttag gggaagaaat tcttgcatat gctggagaat attttaaggg ggtatctgct
     2461 gtaatgtcat atagcgggta tcaaacgggt gtttacggaa caagaaatgt ttgttctcaa
     2521 ataatcaata acgggtatgc ttcgtttgca tttgtttcag atatgtcgac tggttatagt
     2581 gggaatttag gttttcctat gcctagacaa tggtcttttg atcaatttgt tgaatttacc
     2641 ataggaagtg gcaacggagc tgttggtatc gatttaatcg ctacatcggg tagagatagc
     2701 ggatttaatg agttatcaaa tgatactaat aatgataatt acattgccaa atacaatcag
     2761 aaggttataa atcagatggt acgtgcgtat caatatctat cacaagctgg tcttgataat
     2821 ccttggaacc ctcatttgac attttataga tatgtaaatt attctggttt gtcatgggac
     2881 atcatttctt ctcctgtaac agagcatgat agaaaaattt atgatgaata taggaataaa
     2941 ttaactaata gcgagggcct gtataattat tttatagatc ctaatactgg ttcaataata
     3001 ggtttgcctc acttaattgt gacgttacaa tcacagatgt ttctagccga cacaatcaat
     3061 gatagtgttt ctgactttgc aggttggtta ggagatttaa tgacttgctg gggtgaagtt
     3121 aagaaattag gaatacaaat ggagcaaggt gtatttgcat tagtaggtac ggctgcaggt
     3181 ggaacattta gtctagaaga tttataccaa gatgttgacg cggtaaatgt ttatacctta
     3241 gcaaaaagaa attctgattc aacagcttta tcggctttaa aaacgtacta ttgtgataag
     3301 ggtgctattg atagatttaa tcaatttgtt aagaacagat ttggttcttc agataaaatc
     3361 gcttcagtaa caaatagttt attagaaaat gacgaaacag
//
LOCUS       DACDSY010000201         3391 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDSY010000201 DACDSY010000000
VERSION     DACDSY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.204"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(326..688)
                     /locus_tag="IWK44_002750"
     CDS             complement(326..688)
                     /locus_tag="IWK44_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551206.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKH
                     FGFESFDDFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPNESDGLLGVIPSKK
                     SQMSESKFTSMFNDVSLR"
     gene            complement(692..1009)
                     /locus_tag="IWK44_002751"
     CDS             complement(692..1009)
                     /locus_tag="IWK44_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551207.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSTINVGLANSSLITDKFVYGKIAETEDESEKEFWLNILTYVMQLNQIGVIYRNKSKG
                     KEL"
     gene            complement(1110..1343)
                     /locus_tag="IWK44_002752"
     CDS             complement(1110..1343)
                     /locus_tag="IWK44_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551208.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(1340..2314)
                     /locus_tag="IWK44_002753"
     CDS             complement(1340..2314)
                     /locus_tag="IWK44_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9551209.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVG
                     NLKENIYYHTTHDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQ
                     LRRGKATDWHAMVYESLDSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYK
                     YRNLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
     gene            complement(2763..>3391)
                     /locus_tag="IWK44_002754"
     CDS             complement(2763..>3391)
                     /locus_tag="IWK44_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP9551210.1"
                     /translation="SAEMRYISKQREKNEREKALVASEKAKIEEEKKSIIDLEKELAV
                     VFQKDPVPLNSFEKLSDGSYRLSSADFVDLYQKASNATNSKKYADMANESLGNTLDKN
                     IALFDKNMALEQENSFLKNQQKVDVSAVVAENKKLKEENRSLRRRLTQLKNAFQKSIT
                     RLSIRFGLVEKDMGISEELKILDQEKTLASQQNAPERNIGRSENEMEW"
BASE COUNT          975 a          745 c          478 g         1193 t
ORIGIN      
        1 tgcttatgcc aatcattggt tttctggcta tcagttaact cacgattatt atgagaaata
       61 cttgtattct tcgttccttt tttaaatgaa atactaaacc ccaagttaaa accctccttc
      121 gcgctgtcgc taataaaaag agtgtaaacc tttctgttac tctttgtcaa agagtaacat
      181 tttcctaatt gtgacatggc tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca
      241 caagtgacaa cgaaaaaagg agccgaaagc gtgtcacgct ctcaactcct tttacgcttt
      301 attttgcttc acgcaaaata atcgcttatc gtaaactcac gtcattaaac attgacgtaa
      361 atttactctc actcatctga ctttttttac tcggtatcac tccgagtaag ccgtcagatt
      421 cattgggata atctcgttcg cttggcgctg atttttcatc agcaagccac tcattatcca
      481 ctgatttttc gtgttgctga tcgtcagaaa ttgacgataa ccaccgctca aaatcatcaa
      541 aagattcaaa tccaaaatgc ttccgaagtg ccttacccat ttcagcattt tccttctcaa
      601 tcaatcgttt tttctgttga cgaagttttg cctgttcttt tttcaatttt tcttcttgtg
      661 ctttaatatc tgctaaagtc attcccaaga tttacagctc cttgcctttt gatttatttc
      721 gataaataac accaatttga tttaattgca taacataagt taaaatattc agccaaaatt
      781 ccttttcaga ttcatcttct gtttcggcta ttttaccgta aacaaattta tcagtaatca
      841 aagaagaatt tgctaggcct acattgattg tagaattgag aacgtccatc attctctcaa
      901 gcatttcact ataattatct tcatccggca aattcaaata atcagctaat cgttcataca
      961 tcaattcaat tctatcaact gtctcttctc ttaaatcaat tttatccatg ttacacctca
     1021 tttccaaaac tgccaccatt tccgattctt taattttcga tacacctaat tcatcagcaa
     1081 gattttttat ggtcatcttt tctgacaaat cagtcattag cttctccact actttcagct
     1141 accgcataaa tatatttaac ggcgtgctta gggataataa tcggagagtc aattttatta
     1201 tttttcaaaa tcaattcata ttgcgttaca tcaagtaact taccggacat ttttttgcta
     1261 tccaaaaaaa caaccacaac gtaatcacct gttaaattta ccaaccaatt tttcttacca
     1321 aaatactttt gtttttctgt catttttctt cctcctctgt tacgtctagc cctagaagcc
     1381 atgtgtctaa ttcttttttt aaatcgtctg ccgattctgc aaaggtttct cgtaaatgat
     1441 tcgtcaattc atcattttga ctttcttgct tcagcaaatt tcgatattta tattttgtct
     1501 ttcgattaat ttgcccccaa taatcatgtt ctgttgttaa agcagtaatc atcattttgt
     1561 cagctacact tgtatcagca ggcaagtaat gaggactagc aaaactatct aagctttcat
     1621 aaaccatagc atgccaatca gtcgctttcc ctcgtcttaa ttgtaattct aaccgccacc
     1681 aactcacgat ttcttttggc acaatttttc ttttcttctc ttgttccagt tttttattat
     1741 acatccgaat ttgacgttca gaagagcgag atccccaata tgccgtctct aattttccat
     1801 tccgtccata aataggctta aaagaaactg gatcaactac ccgatattgt gtaataaact
     1861 catcaggtac atcgataatg tcgcaagcaa tatctgcacg tgaaaagtga ggttcgataa
     1921 acaaatcatg aataaaattt ttcatggaac tagctaagaa ttgactaatc ttattcggat
     1981 tgaagtctat acgccctttc ccctcttgcc attttaaaat ttcaatataa gcaatattct
     2041 cttcatgttg gtctaacaac tgccaaccat tattactaac agatcttaaa taaccgttac
     2101 cttcatttag gcgcataagc tgttcaaaat ctaaaatcaa cacatcatgt gtagtgtgat
     2161 aatagatatt ctctttaaga tttccaacaa tggtaattcg gtcgattgac cactgaattt
     2221 ttaggggggt gtccataaaa gggacactta accgcctgtt agagggggct gttaattttt
     2281 cccactcatt tatgatcgtg ccttttttac tcaaatttag ttcctcctca aagaaaaaag
     2341 agattggtgg aaagaatttt ccagagttca cgtaactcta aaaaattcat aaccagcaat
     2401 ctctagtctt gaagaaacct gaaattattc gtataatgaa tttgagttac ggagctagtg
     2461 tgattagttt ccgtaaaggt cgagaacgtg tccagcgttg ctcgacttta tttttttgct
     2521 cttttttcag tttatttatt tgataactca ataatagcac ttctctttag ctatgcaaat
     2581 atccaaaaac ataaaaccgc accctttgaa aagggcgccc ccaaacttcg ctaacgctaa
     2641 gttggccaaa aaatggactt gccatttttt agcaaatgtc agcttcaggg attagtggtt
     2701 tagccgtcaa ctccttatcc tcggcacaga ccgcctccgg taccgttgac tgcaccgtat
     2761 aactaccact ccatctcatt ctcagaacgc cctatattac gttctggggc attctgctga
     2821 ctagccaacg ttttctcttg atctaatatt tttagctctt cagatattcc catatctttt
     2881 tctacaagcc caaaacgaat tgataaacga gtgattgatt tttgaaatgc gtttttcagt
     2941 tgagttaacc gccgacgcaa agaacgattt tcttccttca gctttttgtt ttcagcaaca
     3001 accgctgaca catcaacctt ttgttgattt ttcaaaaaag aattttcttg ttctaacgcc
     3061 atgtttttat caaataaagc gatattttta tctaatgtat ttccaagcga ttcattcgcc
     3121 atatcagcat attttttgct gttcgtagca ttagacgctt tttgatacaa atctacaaaa
     3181 tcagcactag ataagcgata agaaccatct gataattttt caaaactatt gagtggcaca
     3241 ggatcttttt gaaatacaac agccagctct ttttccaaat cgataatcga ttttttctct
     3301 tcttcaattt tcgccttctc agaagccacc agagccttct cacgctcgtt tttttctctt
     3361 tgcttagaaa tatacctcat ttccgcagaa a
//
LOCUS       DACDSY010000202         3317 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDSY010000202 DACDSY010000000
VERSION     DACDSY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.205"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            152..364
                     /locus_tag="IWK44_002755"
     CDS             152..364
                     /locus_tag="IWK44_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551211.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(642..2441)
                     /locus_tag="IWK44_002756"
     CDS             complement(642..2441)
                     /locus_tag="IWK44_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9551212.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2565..3161)
                     /locus_tag="IWK44_002757"
     CDS             complement(2565..3161)
                     /locus_tag="IWK44_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9551213.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1210 a          597 c          586 g          924 t
ORIGIN      
        1 aagatcgtgt taaaaaatag ttatctaaaa agcagaaaga tactcaaaat aaaaaataaa
       61 aaaatggata aaaacgtttt caaatacaag gtaatttgtt attataaaat aaagaaaaaa
      121 caaaataaaa ataaagaaaa gagggaatga aatggtaaaa gcagaagtag gaagatctta
      181 tatatgcaaa ccgatcggag tatctcatga actaatcggc tgtattgaac gtacatatat
      241 caacacggct gtcgtgtatg ttgaaagtta tgaccaacga gactatcaac ttatcaatga
      301 aagcaagtat cgcatgctcg taaaattctg tgatattagc gcacctgctg aattggtttc
      361 ttaaacagta tttttgagaa ttcgcaagaa ctcccttata atataaataa agacagaagg
      421 gagcgagaag atatgccaaa aagaaaaagt tcagggaaaa tgggaattat tcttgcagtg
      481 atctctgcaa ttcctgctat tttagctatt gttcgtgcga tgaaggaatc aagagaaagt
      541 taagcacaca ttatgagcgt caaacaaaaa acgcatatac ctgcggtggc aggttatgcg
      601 ttttttgttg tataaagact tttttcatta acgatttttg attagccgac tcccaaaaca
      661 aaatttagca gtttatctga ataagtgatt tcttcatctg tttgtttcac actttcattt
      721 agtagtccca tctcatcggt tatgatacca tccacaccat aaaacatcat gcgactcata
      781 gcatctgaat cgttgattgt ccatacatat acttcttttc cgtcttggtg ggcagaatta
      841 atgaagttac ggttgatcgt actatattcc attgtcagaa aatcagcagg cgtcacaggg
      901 ggcccaacaa tattgaaagg taagatgtag cccacataaa agtttggttc tgcttctttt
      961 aaatctgaaa cggtttggta cgtcaaactt tgcaaaatat gatggttctc taagataact
     1021 ttgcggtatt tatggacaaa tcgttcgaca aggtcatccg aatctttttt agaggtcttg
     1081 atctcaataa gtaatttttg gtttagctta tgtgcttttt ccaaataaga atcaaaagaa
     1141 caaacagggg cttgcactcc gttttcagta actgtaagat ttgtcaattc tttcaatgta
     1201 agatcctgtg gtgttccatt tacccctgtt aagtgacgaa gatttgcatc atgcatcact
     1261 acgaaatgac catcttttgt ttcctgaaca tccatctcta cataatcagg atgatagtct
     1321 ctgcttgttt tttcaagtgc ttctaaagta ttttgcgccc cattttgggt actcacacct
     1381 ctgtgagaga cagtcactgg ttttgtctga acagctgaag tcagataatc catattgtaa
     1441 agacagacgc caataccgaa aaaaactgca aaaagagtca aaccagtgtt tttcagcgca
     1501 gagaaacgca agttgggggc ttgtgggata aaccacttag ggatctcagg caagaagcct
     1561 tcgtcatcca tgaaatcaac aatgatataa aaaatcccta cggtagacat cacgatattg
     1621 aataataaaa tcccttgtaa aagcgtcata gcaaacacag cgctgatcaa tgcatagtct
     1681 gggaaaaatt gttcaaccat actttgtgcc aaaataacaa cgatatatcc taaagaagaa
     1741 aggagtaaaa tcgtaccgcc aatcacaata aattggcccg tgatggccaa aagacgcgat
     1801 ttggtcaatg accagctttc ccgaatggct gctcgaaaag gacgatctcg tagaatcatt
     1861 tccggtaaag caaaaatcag tcgaatccca atatagccaa ggaaaatata aacgagtaaa
     1921 aaggaactga cgatgatcca acgattagca aaaataaaat ccataatgaa agcggggatt
     1981 tttatttttg acagtagatc agaattaaaa cttaatccgc tgatcggtag aatcaataaa
     2041 aaataagcaa ggaaaaacaa aaaagtaatg ggtctcactt tcttcagctg aagtatcgtc
     2101 aagtgtaaga gttgcttcaa agagattggt tctttctttt taataaaaaa aacactcatc
     2161 aaaagaaaag taaattcgaa ataaactaac agtaaaatcg tcatcaaaac tacgattaac
     2221 gagagtagaa ccccaggatg ttgagaaaaa attgaaggaa tattgtcata agacaaatag
     2281 tcaattgccc ctcggtgtaa aataaaacgg gtcgtactgc tcaataaggg taaacaaata
     2341 aataaaataa agccatgcat cagcaagaca tcacggaaat aagcttgtgt tccttttaaa
     2401 aagtcccaag cattacctaa gctacgtttc aaataattca tcctatcacc tgctcttcac
     2461 attgtagcgc aaatctaatg aaaaaggaaa gggtgcagtg tgttttgcag accctttccc
     2521 agatttaaca ttgtcaatca atgactaatt ttacaaaata aatattatcc aagaagacca
     2581 cttagatatt gacctccata aatcaaggcc atactatata agaaaatcga cagtggttta
     2641 gcaatcaaaa tgattagtgt atatcgtttc actgacatgt ttgtcaatcc agccatcaaa
     2701 cataacgcat cgtcaggagc aactggaaga aaaatagcta gagcaaacag cttctcaaaa
     2761 cggttttgat tatccagcca gccaatatat ttgttatatg tcttatcgct aatcatggaa
     2821 agtatgaatg gctttccata acgacgccct agcagaaaaa tgataatcga accaatgcag
     2881 attccaatat agttatagat aaatccagca taagggccaa atatcaggac accagctgca
     2941 gtactgatcc ctccaggaat gattggaata actacttgca ggatttggat gaagacaaaa
     3001 ataatcggtc ctaaaatgat ggaatcacct actaatcctc ttaacgcatt caaatctttg
     3061 aaaacaccta aattaataaa ataaatcgtc agcgcgattg ataggcctaa tccgataatg
     3121 gatatgaaat ttatcagttt tcgagaggca gcaatattca ttattcatca actccttaga
     3181 ggttctttgt taactatact ttactagaaa agcaactaac tgcgccattt tttgatatga
     3241 cagaaaacaa acaattttgt aagttcatca taagtataaa agattgatct caaaaaaaca
     3301 tccaactaaa gacttat
//
LOCUS       DACDSY010000203         3297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDSY010000203 DACDSY010000000
VERSION     DACDSY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.206"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..695
                     /locus_tag="IWK44_002758"
     CDS             <1..695
                     /locus_tag="IWK44_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9551214.1"
                     /translation="NTKVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVK
                     YYDEIANSLTEALYSTIRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFL
                     RVGVQAKAVLDPHFQAQVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAIT
                     NYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELRE
                     KVLRSIIDKSIE"
     gene            925..1155
                     /locus_tag="IWK44_002759"
     CDS             925..1155
                     /locus_tag="IWK44_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP9551215.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(1378..1476)
                     /locus_tag="IWK44_002760"
                     /pseudo
     CDS             complement(1378..1476)
                     /locus_tag="IWK44_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1741..2196)
                     /gene="tnpA"
                     /locus_tag="IWK44_002761"
     CDS             complement(1741..2196)
                     /gene="tnpA"
                     /locus_tag="IWK44_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP9551216.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2439..3297
                     /locus_tag="IWK44_002762"
                     /pseudo
     CDS             2439..3297
                     /locus_tag="IWK44_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1085 a          576 c          576 g         1060 t
ORIGIN      
        1 aaaacacaaa agttggcgat gctactatta ttcgcttttg tcaaaaaatt ggtttttctg
       61 gtttttctga tttaaaaatt gaaattgcaa aggaagactt tagtcaaaaa aaagaacatc
      121 cgactagtgt aaagtactat gatgaaattg ctaatagttt aacagaagct ctctattcca
      181 ctattaggct tttagacgaa gaaaaattga atgaagccat taaaatgatc actcaatcaa
      241 aaaacatata tatatttgga gtgggatcta gtggaaatac tagtcttgat ttagaaaata
      301 tgtttcttcg cgtaggagtt caagcaaaag ctgtactaga ccctcatttt caagcccaag
      361 tcgcttcttt attaacagtt aatgatttag taatcatttt ttctttatct gggaaaacga
      421 aagatactta tgattcattg aaaatagcta aaaaaaatgg cgcaaaaatt attgctataa
      481 ctaactatat tcattcacct ataggaaagt ctgcagactt agttttacaa acagccattg
      541 aagaatttct taatggtgga tctttagctg gaaaaatatc acaactttat atttgtgatc
      601 tattagttca tggttatgaa caaaacaata atattgattc agttgaatta agagaaaaag
      661 ttttgcgctc tattattgat aaaagtattg agtgaactac ccccacttag ctaaacctaa
      721 cggtttttaa cgattgaagt aggcaattct tgggatgaga gcatacttga tagcttattt
      781 tttactaata gcggtggctc ttattgacca aatatccctg tagttcctac ggttcttgat
      841 tttatctaaa gatattttta tcgtggaagc tattgatcaa cttgaaagaa aaacgtgtag
      901 ctcattatca ctagtttatc gagtatggat gaagcaatgg atcatcctct cttaaataaa
      961 tttattaagg atctgatcct tcaaattttt tccatgatcg atgagcagga acctactgaa
     1021 tctaaacgac atcgggcaca aggaattatt aagattgcta agcgaatgtt ttctataaag
     1081 ggtgttctaa gctatacagt gttaattcaa aagattccca atcatcttgt ttataagaac
     1141 aacgttgaag attaaaaaag tgtttcaatg ctaaaattgc aaaatacttc aatgttatga
     1201 gacaaactgt ctataagaat caatttccac ccatttcatc acttgttaaa agtttatgat
     1261 ccttttgatt tttatattaa tttatctgta tttaatacag attttattta ttagttcatc
     1321 tgcctattgt attttatttt gttgccttac catctgaagt aaatatactc ttaattgtca
     1381 aattaagtta gacagatcat caatacttac gtaactcaaa aatacttcca aaggtgttcg
     1441 ataatccagt gattttctag gaagattatt tctgcgccag cattttctaa tgtttgaatt
     1501 tgtgttttgt aatcttggtt gcttgtgcta attcttgaat agccgtaaat tgccaaaatt
     1561 tcatcaccct ttctggacat aagtttctga caccagttaa aatcaagtct atcagtaacc
     1621 catacacccg tgaggacggg tgtattttta atccccctag aaggggtcga cactcgctgc
     1681 cccttaaaag gggattttta aaagaccgcc aactgctcat cttcactcac ttacttctct
     1741 ttatttttta aatggttcta cgtactctct tttacttata atatatcgaa tgcgatcatc
     1801 agcttcttgt tcacggatgt atttttcatt tgtcttttga ttcaatccga ctgtactcac
     1861 atagtatctc ttcgaccaaa aggatttgtt tccgtattta tatttgagat ttgcgtgctg
     1921 ctcgtggatt agtaccgcac tacgaccttt cagatacccc ataaaactgg atatacatat
     1981 ttttggtggt actcgaacaa gcatgtgaat atgatctagc attgcatgtg cttctatgat
     2041 ttccacatct ttcatttcgc acaacttcct aagtattttt ccaagatctt ttcgtaattg
     2101 cccatagatt acttttcgtt gatattttgg tgtaaaaata atatgatact tacaattcca
     2161 acgagtatat gctaaacttt tatcgtcatt tgacatgaaa aaactccttt cgatctgatg
     2221 agcggttggt agccactcac tagtatatcg aaaggagttt ttatcgctaa agatacttct
     2281 tccacgcgta gaaacgcgcg gttttcttgt ttcgcacgct ttacgcacaa aactgactga
     2341 agtcattaag aaaataaaag ggtctaaaac ttttaagttt tgtaccgacc cccaaaagtt
     2401 agaccaaaaa atctaacttt tggaggtcgg tgttttttat ggctaagtat acttttgaac
     2461 ttaaattaaa gatcgttcat gattacttgg atggtaaagg cggaagtgat tatctggcaa
     2521 agaaatattc cattaaggct ccatcacaag tgaagcgctg gatcaatgcc tatcaagaat
     2581 ttggtgaaga gggtctagta agaaagcgac aaaagaaaaa atattctgtt caatttaagc
     2641 tggatgcgat agagttatat ctaacaagtg agctgtccta tcgtgaagtt gccaacacac
     2701 ttaacatgaa caaccctaat ttgatcgcta gttggatgcg tcaattccgt gagggtggga
     2761 tcgatggact ctcaaaaacg aaaggatgtc cacctatctt gtctaagaaa aatgaaccca
     2821 aaaataagaa aaaatctaca actaaagcaa cgtctaaaga acgtgaacgc atcgaggagt
     2881 tggagaaacg agtccgttcc cttcaaattg aaaatgcctt tttaaaagaa ttgaggaagc
     2941 tgcgaaaaca ggaagcccaa caacgacgaa tgaagcaatc gcacgaatca tcgcaagcct
     3001 ccgaggaccg tttaaattag ttgagcttct agaaacttta aagttcccta aggcaatctt
     3061 tatgtactgg caaaaacgat tggaccgtaa aaatccagat caagagattg aagcggaaat
     3121 gcttaaagtt cgggagaagc acaaggatta tggctgttta cggatgacca atgaactaag
     3181 aaaccgcggt ttctctatca ataaaaagaa agttcagcgc ctgatcaaga agctaggaat
     3241 aaaagtaacg acatacacac gtaaatcacg gcgctacaat tcttatcgag gacaagt
//
LOCUS       DACDSY010000204         3279 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDSY010000204 DACDSY010000000
VERSION     DACDSY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.207"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            247..555
                     /locus_tag="IWK44_002763"
     CDS             247..555
                     /locus_tag="IWK44_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9551217.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            582..881
                     /locus_tag="IWK44_002764"
     CDS             582..881
                     /locus_tag="IWK44_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP9551218.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            1055..1606
                     /locus_tag="IWK44_002765"
     CDS             1055..1606
                     /locus_tag="IWK44_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP9551219.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1752..2045
                     /locus_tag="IWK44_002766"
     CDS             1752..2045
                     /locus_tag="IWK44_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551220.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            2038..2334
                     /locus_tag="IWK44_002767"
     CDS             2038..2334
                     /locus_tag="IWK44_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551221.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            2405..>3279
                     /locus_tag="IWK44_002768"
     CDS             2405..>3279
                     /locus_tag="IWK44_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9551222.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSY"
BASE COUNT         1233 a          465 c          649 g          932 t
ORIGIN      
        1 ggatgaaacc tttattcaat ccgttgcctc gaaaagaaac catattccaa gaaaatcatt
       61 aaattatcga acaccattgg aagtattttt gagctgcata gataaagaca tattgtctag
      121 cttaatttga caaatcagat agtataaaat gaatcaaatt gaatatcaaa tatatattat
      181 atccatgaag cccaatcttc tttaatagaa gattgggcta tttttatggc aaaggaggga
      241 aaagaaatgt ttcaaaataa aggacaacgt tatgtcacaa agggagtaat ggacagtttg
      301 ccggtggaat tacaagcttt atgttggaat ctaatcgatc aaaacgttca aaagcaattt
      361 catttagact atcttcaaat ctttgaattt tctacggaaa aaggaaatca aaagttggtt
      421 catcgtcaag aagaaccaga agcaagaaaa gagtatctga tttcacctaa gcttcgtttg
      481 aaatcagtgt ctcaaaaaat atgggtcatt gattcgggtg agtatcaaac gatgttatta
      541 ccagaagaat actgatatct aaaagaaagg aacgaaagga cttgttagaa acggctatcg
      601 aagtactgga aaagtgtgca cagctagtca ctactcctga agagtggggc tatgaatccg
      661 ttacgatgga aaaagaggaa attgaaatgg gtatgcttcc tgaagatgtt actctaccac
      721 gtttagtgat gacacatctt tatatttatt gcgcaccaga agatggaaaa gactatgtag
      781 tttatttcat tacagatatt accagccagc gagaatttgt tcgtgggtta ctggtagaag
      841 gacgattggt ttggagtcag attggaggaa cgaatgaata agatcgatta tttgagaatg
      901 attgcgaaat gtttagaaga tatccttaca gcgattcatc aaattctgaa gaaaaaagga
      961 gaaagaatat gaaatatttt caactagtat tgacacttat aacggcaatg gtgattgaat
     1021 tgcttgaaca aaaattagca taggagaaaa agagatgatt gaaaaagaat tagtgaccac
     1081 acaaacagaa gtttggcgag atcctcatca tacccatcgc tatgttttca aacgacaatg
     1141 ggcgatcaaa ggaaaagaag cggaagataa attagcggta gtcattacta ttcgtccaac
     1201 agatacagaa ccatttacga atgatttgag tatgttatta atcgaaaaga atatccgaca
     1261 attaggattt actggtttta ttgctgtgaa tcttttttcg tatacaaaag cgaaaagtcc
     1321 tcgggtattt cctcgtggaa atgatgaaca atctttggaa atattaacaa ctgttttcac
     1381 agaaaagaaa atcaacacca ttatttttgc ttgtggttcg attacagcga cgagtcaagt
     1441 ggcttatgaa caagcgaaga ccatttacga tcaattgagt gcgaaacaaa aaaagatgac
     1501 caaagtattg ataaatgcag aaggtaagtt atcccaccca ctaagtattc atgttcgtaa
     1561 tgaatggcaa ttggcagata gtcaattatt atttcaagaa aaataacatt ttattttctt
     1621 atgttactta taagtagttt tacatgcttt ttgactttaa aaatacatgt gatgatacat
     1681 gttatatgaa ttatcaatgt acccagtaat atgctatttt cattatgtaa aaaataacag
     1741 ggggtgtgta gatggagatt gatgaaactt gtttgagtaa ctcttataaa agtatgaatg
     1801 acttttttga ggaaatggaa ctggattcag aattgctcta tcaattttat cttgcttttc
     1861 gagggcaaca gatttctttt ccaatgaaaa tgtacgatcg tgagttagtt agaaaaagaa
     1921 tcgaaaacat gatagaacag gaaaaaactg tagatatccg tcaattgaca gaagtttatg
     1981 gtttctctgc aagatggatt caaaaagtaa ttaaacaaaa agaaaggaga cgagtacatg
     2041 gctaagctga ttgtattagg tggtttaatt tacggtggat attggttata taaatatatg
     2101 cttgcgcatc cgaatataga taaaggaaaa gtaattaaga cagttggggt aatcatagtt
     2161 ggactcatag ctctaactaa gaaaaataat actaatagcc caaaaataat gagaacttat
     2221 cgctgtagag gatgtggaag ggaaatttct actagtttta atccatctga aagccctcaa
     2281 ggaagagatc gttgtcatgc tggctatcga cattcttggg aaaggattaa ctaaatgaac
     2341 accattgaga ttaattgact gataaaataa gtacgcgagg gagaaattga aagggggaaa
     2401 taaaatgaaa aaatggttgt tattactaat tggaatgggg attttattca ctggttgtac
     2461 atctgagaat acgaggacta aagagactgt agaaactgat acaacaattg aggctaaaaa
     2521 aagtaaaacg caatcaactg aacaaactac aacagaaatg aaacaatctg tggaagcaag
     2581 tacaagtgaa agttcaatca ttgaatcttc ttctgaagag gttaaaaata ctttgtggga
     2641 tagtgagaaa tctactcaac tagcaatttt tgtcccacaa tggggaaaga cattaggtca
     2701 agaatataaa agctacaatc ctcagaacaa tgtaagtctg tatggtacgc ctttaccaag
     2761 tgcagttatt gatggaaatt ggaaaatggc aatcaatgaa gcaccagtta ccgtacaatg
     2821 gtcagaagat ggaactggtc atgcagattt caatttagta gcagtttatt ctgatgtaga
     2881 aactggagaa tatttagggc agcatgtata tttctttggc tttcaaaatg gacaacctaa
     2941 agtttattta acgcaacaaa atcaaggcaa tgataaaaac tacatgtact tcaatgaaac
     3001 tcagaaccaa cagttaaaac aaggtttcat tgatattgtg aatggacaaa ctcctcaaac
     3061 tccagtagta gaagaatcta ctcaacaagc aacaactaca acgacacaat ctacaagtag
     3121 ctcagcaatt tcaagttggg aagctgctaa agaaactgta ctaaataata aagctttatg
     3181 gttgtatgat tctcctgata aaaatgcatc aaatactaat ttaattagca caaatgaata
     3241 tatgaaagat ctaatgcaag atgaaaatgg aagctacta
//
LOCUS       DACDSY010000205         3249 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDSY010000205 DACDSY010000000
VERSION     DACDSY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.208"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..495
                     /locus_tag="IWK44_002769"
     CDS             <1..495
                     /locus_tag="IWK44_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9551223.1"
                     /translation="KKLINSIRKLNDAQVLELQTLKPNDLVVIRLELNGKKVSLSNAS
                     AGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVRSIITKKENRQILIVTHNA
                     NIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTVMEGGEDAFTNRAKRYGFK
                     QIVE"
     gene            545..934
                     /locus_tag="IWK44_002770"
     CDS             545..934
                     /locus_tag="IWK44_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9551224.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            921..>3249
                     /locus_tag="IWK44_002771"
     CDS             921..>3249
                     /locus_tag="IWK44_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9551225.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         1051 a          545 c          698 g          955 t
ORIGIN      
        1 aaaaaattaa tcaattctat aagaaaattg aatgatgcac aagttcttga attacaaacc
       61 cttaagccaa atgatttagt agttatccga cttgaactaa atggaaaaaa agttagttta
      121 tcgaatgcta gtgctggtca aaagacatct gcaattcttt ctatgatttt ggcgtatgga
      181 aattcaccac tcatattgga tcagcctgaa gatgatttgg atagtcaatt aattaataac
      241 ttaatcgtaa gaagtatcat tacaaaaaag gaaaacagac aaattttaat tgtaactcat
      301 aatgcaaata tcccagttaa tggtgattca gaatggttga ttagcatggg agattcaaaa
      361 gaaatatcgg ctgatcagtg cgggtcggta gatgaaccca atatcaagcc aagaatatgt
      421 actgtgatgg aaggtggcga ggatgctttt acaaatagag cgaaacgcta tggttttaaa
      481 caaattgtag aataacacta gagggtgaga catattatta tgtccgccct ttttaggagg
      541 aaaaatgaaa aaaataaaaa gctataccag tatctgggca gttgaaaaag tcatttacgc
      601 aatcaatgat tttcagttgc cttttcctgt gacttttaac caaatggctt ggttcgtttt
      661 atctttactg tttgtcatcg tatttgccca tgtgcctcca ctatctatga tagaggaggt
      721 ctttttaaaa tacttaggca taccggtggc tgtcacttgg tttatgtcgc aaaagacgtt
      781 tgatggcaag aaaccactgg gatttttacg atcctttatc agctatcact tgcgatttaa
      841 ggtgactttt gcaggaaaga aagtcaaaga acagaagaaa cgatgggatg aacccagtac
      901 tcttgtaagg agtgtgaact atgtatccga ttaagtatat tgaaaataat ctcgtcttta
      961 accaagaagg cgagtgtttt gcctactatg aattagtgcc ttacaattac tcctttttgt
     1021 cacccgaaca gaaatttcaa gtgcatgata actttcgcca actaattgcg caaaatcgtg
     1081 aaggcaagat ccatgcgtta caaattgcca cagaaagtag tattcgcgcc acacaagaac
     1141 gatcaaaaaa agaaattacc ggtagactca aagaagtagc gaaacaacga attgatatac
     1201 agacagatgc attagtatcc atgattggcg atagtcagat tgattatcgc ttttttattg
     1261 gctttaaact aattgcgaca gatgaagaag tcaatctaaa gagtctcaag aaatctttct
     1321 tttctgggct tcaagaattt gtctatggag tgaatcatca tttgatgggc gattttgttt
     1381 ccttatccaa tgaagaaatt cgctgctatt ctaagcttga aaaattaatg gaaagcaaat
     1441 tggctcgaag atttaaagtc aggcgagtca caccgagtga cctgacgtat ttgattgaac
     1501 atatttatgg cgaaaaaggg ataccgtttg aagaatacga atttcagcta ccaaagaaaa
     1561 agctaaaatc agaaacactg gtgaaacgtt atgacttatt acgtccaaat cgttgtttaa
     1621 ttgaagaaaa accaaggtgc ttacggatgg aacatgagaa tcatgagtcg tacgtagcgt
     1681 atttaacaat taatacaatt gtgggggaga tggaatttcc ttcctcagaa cttttctatt
     1741 accagcaaca acaatttaca ttcccaatcg acacaagtat gaatgtagag attgtgacga
     1801 ataaaaaggc tttagctacc gttcgtaata agaaaaaaga actgaaagat ttagacaatc
     1861 atgcttacca atctgacaat gaaacgaatt ccaatgtctt agatgctttg gattccgtgg
     1921 atgaattgga aaccaccttg gatcagtcaa aagaatctat gtacaaactg agttatgtgg
     1981 ttcgagtgag tgccgaatca gtggatgaac taaagcgacg ctgtgatgaa gtgctcgatt
     2041 tttatgatga taccaatgta aaactagtcc ggccctttgg cgatatgatg gggctacatg
     2101 aagaattctt accctcaagt aaacgatata tgaacgacta tattcagtat gttacgtctg
     2161 attttttggc ggggcttggt tttggggcta ctcagatgct aggagaatta gaagggattt
     2221 actttggcta caatgtggat acaggtcgta atgtttacct gaaaccggca ttggcttcac
     2281 aaggagtcaa gggttcagta accaatgcgc tggccgccgc tttcctgggt tctcttggtg
     2341 gaggtaaatc ctttagtaac aatctcttag tctactatgc tgttttgttc ggtggtcaag
     2401 ctgtgattgt cgatccaaaa ggggagcgag gagggtggaa agaaaccttg ccagaaattg
     2461 cggatgaaat cagtatcctc aacttgacga gtaagtcaga aaatcaaggt ctgcttgatc
     2521 cttatgtgat tatgaagaaa aagaaagact cggaaagttt agcaattgat attctgactt
     2581 ttttaacagg aatttcaagt cgagacgggg agaagtttcc tgtgttacgt aaagccattc
     2641 gaacggtgac tcaaagtgat caacgggggt tattacaagt tattaacgaa ttgcgaaaag
     2701 acgggtcacc aatcgcggaa aatatcgccg atcatatcga gtcctttacc gattatgatt
     2761 ttgctcattt gcttttctca gacggctcga ttacccaatc gattagttta gataaacagc
     2821 taaatatcat tcaagtggcg gatttggttt taccggatgc agaaacgact tttgaagaat
     2881 ataccaccat ggaattgctt agtgtttcga tgctaatcgt catttccact tttgccttgg
     2941 actttatcca tagtaaccga gaaatcttca aaattgtcga tttggatgag gcttggagtt
     3001 ttcttcaagt ggctcaagga aaaacgttat cgaataagct tgttcgtgct ggtcgggcca
     3061 tgaatgctgg ggtctatttc gtcactcaaa atagcgacga tttattagat gaacgtttaa
     3121 aaaataatat cgggttgaag tttgcttttc gcagtaccga tattcatgaa atcaagaaaa
     3181 ccttggaatt ctttggtttg gatcaggagg atcaaagcaa tcaaaaacgc ctgcgagatt
     3241 tggaaaacg
//
LOCUS       DACDSY010000206         3160 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDSY010000206 DACDSY010000000
VERSION     DACDSY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.209"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(552..2165)
                     /locus_tag="IWK44_002772"
     CDS             complement(552..2165)
                     /locus_tag="IWK44_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP9551226.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..>3160)
                     /locus_tag="IWK44_002773"
     CDS             complement(2306..>3160)
                     /locus_tag="IWK44_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9551227.1"
                     /translation="TYSIDTHLHLSAAPYFRLLINESANLYAEFAHKSIELDNPSLLF
                     IDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFSIDQEITSIITFLKNEQFK
                     IDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCLQKIQLSSLPRHYQQELAL
                     LTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLLEQLAQIFFISPASLKKHF
                     RKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGFHSIHHFSSAFKKYTGLSP
                     SKYYLSLK"
BASE COUNT         1022 a          547 c          558 g         1033 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt
//
LOCUS       DACDSY010000207         3153 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDSY010000207 DACDSY010000000
VERSION     DACDSY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.210"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(326..688)
                     /locus_tag="IWK44_002774"
     CDS             complement(326..688)
                     /locus_tag="IWK44_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551228.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(692..1000)
                     /locus_tag="IWK44_002775"
     CDS             complement(692..1000)
                     /locus_tag="IWK44_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551229.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(1009..1428)
                     /locus_tag="IWK44_002776"
     CDS             complement(1009..1428)
                     /locus_tag="IWK44_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551230.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(1421..1654)
                     /locus_tag="IWK44_002777"
     CDS             complement(1421..1654)
                     /locus_tag="IWK44_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551231.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
     gene            complement(1657..2625)
                     /locus_tag="IWK44_002778"
     CDS             complement(1657..2625)
                     /locus_tag="IWK44_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9551232.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVG
                     KLNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKW
                     QEGKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRII
                     DPVSFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQ
                     LRRGKATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYK
                     YRNLLKRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
BASE COUNT          918 a          699 c          436 g         1100 t
ORIGIN      
        1 tgcttatgcc aatcattggt tttctggcga tcagttaact cacgattatt atgagaaata
       61 cttgtattct tcgttccttt tttaaatgaa atactaaacc ccaagttaaa accctccttc
      121 gcgctgtcgc taataaaaag attgtaaacc tttctgttac tctttgtcaa agagtaacat
      181 tttcctaatt gtgacatgga tgtcacaatt ccaaacgctc tccgagggtt ttcgctgtca
      241 caagtgacaa cgaaaaaagg agccgaaagc gtatcacgct atcaactcct tttatgcttt
      301 attttgcttc acgcaaaata atcgcttatc gtaaattcac gtcattaaat attgacgtaa
      361 atttactctc actcatctga cttttcttat tcggtatcac tccgaataag tcgtcagatt
      421 catcgggata atctcgttcg cttggcgctg atttttcatc agcaagccac tcattatcca
      481 ctgttttttc gtgttgctga tcgtcagaaa ttgacgataa ccaccgctca aaatcagcaa
      541 aagtttcaaa cccaaaacgc ttgcgaagag ccttgcccat tgcagcattt tctttctcaa
      601 tcaagcgttt tttctgttgg cgaagtttcg cttgttcttt ttttaatttt tcttcttgcg
      661 ctttaatatc tgctaaagtc attcccataa actacatctc cttttctttg gaattaataa
      721 cggaaatttg gtttaactgc ataacatgac tcattaattt caaccaaaat tctttttctg
      781 attcatcagt tgtttgcgct aaatgcttat gaacaaattc attagtcaaa actgaagatt
      841 ttgctagatc agcattgata accgagttga gaacatccat aattcgttca agcatttcac
      901 tataattatc ttcatccggc aaattcaaat aatcagctaa ccgttcatac atcaattcaa
      961 ttctatcgac tgtttcttct ctcaaatcaa ttttatccat gtcacacctc acttccagaa
     1021 ttgccaccat tttcgatttt ttaattcttg attttctagt aacaactgat cgtttttttg
     1081 ttgctgaagc aaagtcaatt tttgttggtc acgaatcatt tccgttaatt cagaaatatg
     1141 cctatctttt tccgctaatt gttcacgcaa aaattcattt tctttttcta acgggaaatc
     1201 attgcctgca tcattgaccg gtaaatcacc agtcaattga ccaccttttg accgataaat
     1261 tgaccgcaca acaccaatca attccttgtc taacatgata accccattat ctttataccg
     1321 agcaaggtca taacctgcat tgaccagttg attgcgcact gtatttttag atatacccaa
     1381 ctcatcagca aggtttttta tggtcatttt ttctggcaaa tcagtcatta tcttctccgt
     1441 tactttcagc taccccataa acatatttaa cagcatgctt agggataatg attggggaat
     1501 caattttatt atttttcaaa atcaactcat actgcgttac atcaagtaac ttaccggaca
     1561 tttttttgct atccaaaaaa acaacgacaa cgttatcacc tgttaaattc accaaccaat
     1621 ttttcttacc aaaatgcttt tgtttttctg ccattactat tcctcctctg tcacatcaag
     1681 tccctgtaac caagtatcca attctttttt taaatcatct gctgattctt caaatgtttc
     1741 tcgtaaatga ttcgtcaact catcattttg actttctcgc tttaataaat ttcgatattt
     1801 atatttcgag tttcttgaca aaatcgccca attagaatga ttagctaaca atccatcaat
     1861 cataacttta tccgtaggtt ttatatcagt tggtaaaaaa tgtggactca caaagctatc
     1921 caaactttct ttcaccatag cataccattc cgtagcttta ccacgtctta actgcatttc
     1981 taaccgccac caagtattaa tttctgaagg caccaccttt cttttcattt cttgctctat
     2041 ttttttattg tacaaacgaa tttgccgctc gcttgcccga ctaccccaat acaccgtttc
     2101 cagttttcct gagcgtccat aaattaattt aaatgaaact ggatcaataa ttcgatattg
     2161 cgtgataaac tcatcaggca catcaataag atcgcaggca acatccgctc gtgaaaaatg
     2221 gggctctaaa aataaatcat gaataaaatt tttcatggaa tccgctaaaa atcgactgat
     2281 tttattagga ttaaaatcaa tccgcccttt tccttcttgc catttagaaa tttcaatata
     2341 ggcaatattt tcgtcatgtt ggtctaatag ctgccaacca ttatttccaa ccgcttttaa
     2401 atagccattg ccttcattta cccgcataag ctgttcaaaa tctaatatta atacatcatg
     2461 ttgagtatga taaaaaatat tttgattcaa ctttccaaca atggtaattc ggtcaatcga
     2521 ccaatttaat tttatagttt gtaccaaagt ttgaacattt aaccgcctgt tagacagggc
     2581 tgttaatttt tcccactcat tcacgattgt tcttttttta ctcatattca gttcctcctc
     2641 aaagaaaaaa gagatttttg gaaagaattt ccagagttca cgtaactcta aaaattcata
     2701 accatcaatc tctagtcttg aagaaaccta aaaatattcg tataatgaat ttgagttacg
     2761 gaactagtgt ggttagtttc cgtaaaggtc gagaacgttg gtagcgttgc tcgacttatt
     2821 tttttgctct ttttccagtt catttgtttg ctaactcaat aatagcactt ctctttagct
     2881 atgcaaatac caaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
     2941 acgctaagtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcaggggct
     3001 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
     3061 acatcgtggc taccactcaa tctcattctc agaacgctct aatttatgct gtgagacgtt
     3121 ttgacgataa aataaggttt tctcctgctc taa
//
LOCUS       DACDSY010000208         3128 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDSY010000208 DACDSY010000000
VERSION     DACDSY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.211"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            103..1347
                     /locus_tag="IWK44_002779"
                     /pseudo
     CDS             103..1347
                     /locus_tag="IWK44_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="IWK44_002780"
     CDS             1360..1992
                     /locus_tag="IWK44_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9551233.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="IWK44_002781"
     CDS             2197..2424
                     /locus_tag="IWK44_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP9551234.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="IWK44_002782"
     CDS             2493..2759
                     /locus_tag="IWK44_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9551235.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="IWK44_002783"
     CDS             2749..3018
                     /locus_tag="IWK44_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9551236.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DACDSY010000209         3127 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDSY010000209 DACDSY010000000
VERSION     DACDSY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.212"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..940)
                     /locus_tag="IWK44_002784"
                     /pseudo
     CDS             complement(1..940)
                     /locus_tag="IWK44_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1161..1523)
                     /locus_tag="IWK44_002785"
     CDS             complement(1161..1523)
                     /locus_tag="IWK44_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551237.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(1527..1835)
                     /locus_tag="IWK44_002786"
     CDS             complement(1527..1835)
                     /locus_tag="IWK44_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551238.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(1844..2263)
                     /locus_tag="IWK44_002787"
     CDS             complement(1844..2263)
                     /locus_tag="IWK44_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551239.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQSTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(2256..2489)
                     /locus_tag="IWK44_002788"
     CDS             complement(2256..2489)
                     /locus_tag="IWK44_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551240.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(2486..>3127)
                     /locus_tag="IWK44_002789"
     CDS             complement(2486..>3127)
                     /locus_tag="IWK44_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9551241.1"
                     /translation="NPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFIT
                     QYRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWW
                     RLELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAK
                     NTKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT          869 a          703 c          436 g         1119 t
ORIGIN      
        1 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
       61 tcttcaatct tcactctctc agaagccacc agagcctttt ctcgctcatt ttcttctctt
      121 tgcctagaaa aatatacctc aactcatcag aaacgctctc acgctctttt acggccgttt
      181 gacgcaattc tgaaatctcg ctcatgattt ctttatattc gtgaacatct ttgattttgt
      241 tagtaccgac taactcacgc tcccaaccaa aatctgccat caaacgttca attgactcaa
      301 cttcctgatt cctaaaatta ttccaaatca ctcgaccatt ctctttatct gctcgtaaac
      361 cctgctgttg caaggctttg ttaaaacttg gttgcttttg aacaccccgt ttataccctt
      421 cagcaacagg aacaatattc aaatgcaggt gcggacttac ttcgtcatta tgaataacag
      481 cattgtagac ggataaatta ggattccgtt ctttaaattc tttgaaatat tcaactaaaa
      541 ttttgttggc aatcactcga ttttgttctg cttgttgttc cgatatgcca gtagaagaac
      601 catcatcacg agtagtacga aaatcatcaa gagtaccaat ttgcacgata aattctcgct
      661 gtggttccaa ttttttatcc ttgcgaacct ttgctaaata gtcctcgatt tttctatcgg
      721 ctcttttctg ctttgcatta tatttttcta cggcttcacc gaataattct tcatattttt
      781 cacgaatatt tgtttgttct aaatagatat tttcatccga ttttgtgaaa tcaatatgct
      841 tatgccagtc attgattttt tggctatcag ttaactcacg attattatga gaaatgcttg
      901 tattctttgt tccctttttg aacgaaatac tgaaccccaa gttaaaaccc tccttcggct
      961 gtcgctaata aaaagagtgt aaacctttct gttactcttt gtcaaagagt aacattttcc
     1021 taattgtgac atggctgtca caattccaaa tgctctccga gggtattcgc tgtcacaagt
     1081 gacaacgaaa aaaggagccg aaagcgtgtc acgctctcaa ctccttttac gctttatttt
     1141 gcttcacgca aaataatcgc ttatcgtaaa ttcacgtcat taaatattga cgtaaattta
     1201 ctctcactca tctgactttt cttattcggt atcactccga ataagccgtc agattcatcg
     1261 ggataatctc gttcgcttgg cgctgatttt tcatcagcaa gccactcatt atccactgat
     1321 ttttcgtgtt gctgatcgtc agaaatcgac gatagccacc gctcaaaatc agcaaaagtt
     1381 tcaaacccaa aacgcttccg aagagccttg cccattgcag cattttcttt ctcaatcaag
     1441 cgttttttct gttggcgaag tttcgcttgt tcttttttta atttttcttc ttgcgcttta
     1501 atatctgcta aagtcattcc cataaactac atctcctttt ctttggaatt aataacggaa
     1561 atttggttta actgcataac atgactcatt aatttcaacc aaaattcttt ttctgattca
     1621 tcagttgttt gcgctaaatg cttatgaaca aattcattag tcaaaactga agattttgct
     1681 aaatcagcat tgataaccga gttgagaaca tccataattc gttcaagcat ttcactataa
     1741 ttatcttcat ccggcaaatt caaataatca gctaaccgtt catacatcaa ttcaattcta
     1801 tcgactgttt cttctctcaa atcaatttta tccatgtcac acctcacttc cagaattgcc
     1861 accattttcg attttttaat tcttgatttt ctagtaacaa ctgatcgttt ttttgttgct
     1921 gaagcagggt caatttttgt tgatcacgaa tcatttctgt taattcagaa atatgtttgt
     1981 ccttctcagc taattgatca cgcaaaaact cattctcttt ttctaaagga aaatcactgc
     2041 ctgtgttatt gaccggtaat tgaccagtcg attgatcact ttttgaccga taaattgacc
     2101 gaacaacacc aatcaactct ttatctagca tgataatgcc attttcttta tactgagcaa
     2161 ggtcaaaccc tgaattgacc agttgattgc gcactgtatt tttcgataca cccaattcgt
     2221 cagcaagatt ttttatggtc atcttttctg acaaatcagt cattagcttc tccactactt
     2281 tcagctaccg cataaatata tttaacggcg tgcttaggga taataatcgg agagtcaatt
     2341 ttattatttt tcaaaatcaa ttcatattgc gttacatcaa gtaacttacc ggacattttt
     2401 ttgctatcca aaaaaacaac cacaacgtaa tcacctgtta aatttaccaa ccaatttttc
     2461 ttaccaaaat acttttgttt ttctgtcatt tttcttcctc ctctgttacg tctagcccta
     2521 gaagccatgt gtctaattct ttttttaaat cgtctgccga ttccgcaaag gtttctcgca
     2581 aatgattcgt caattcatca ttttgacttt cttgcttcaa caaatccctg tattttttct
     2641 ttgtattctt agctagattt ccccactcag aatgatcata aagcaaacct ttcaccataa
     2701 tccgttcagt aactttagta tttggcggaa aataatgtgg actagcaaag ctatccaaac
     2761 tttcatgaac catctcatgc caatcagttg cttttcccct tcgtaactgc aattctagtc
     2821 gccaccatgt cgttatttcc ggcggaacaa ttttacgttt tctttcttgt tccagttttt
     2881 tattatacat ccgaatttgt cgttcactgg cacgactacc ccaatatgcc gtttctaact
     2941 taccagaacg cccataaatt ggtttaaaag acacaggatc aactacccga tattgtgtaa
     3001 taaactcatc aggtacatcg ataatgtcgc aagcaatatc cgcacgtgaa aagtgaggtt
     3061 cgataaacaa atcatgaata aaatttttca tggaactagc taagaattga ctaatcttat
     3121 tcggatt
//
LOCUS       DACDSY010000210         3100 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDSY010000210 DACDSY010000000
VERSION     DACDSY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.213"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            86..1093
                     /locus_tag="IWK44_002790"
     CDS             86..1093
                     /locus_tag="IWK44_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9551242.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1105..2109
                     /gene="neuB"
                     /locus_tag="IWK44_002791"
     CDS             1105..2109
                     /gene="neuB"
                     /locus_tag="IWK44_002791"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP9551243.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2106..>3100
                     /gene="neuC"
                     /locus_tag="IWK44_002792"
     CDS             2106..>3100
                     /gene="neuC"
                     /locus_tag="IWK44_002792"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP9551244.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETAL"
BASE COUNT         1166 a          393 c          591 g          950 t
ORIGIN      
        1 attattgata attagtttag ttatatattt tttaaagatt aagaatcgaa ttatagatga
       61 ctataattca taggaggaga tataagtgtt taaaggaaaa acaatattag taactggcgg
      121 aactggttcc attggaagtg aaattgttcg tcagctttta aaatatgaac caaaagctat
      181 cagaatttat agtagaggtg aagaaaaaca attttatatg caacaagagc tctcaaatca
      241 cactaatttg agatttttaa ttggtgatat cagagactct caaaggattg attatgcatg
      301 tcgtgatgtg gatattattt ttcatgctgc cgcaatgaaa cacgttccag caagtgaata
      361 taatcctatg gaagctgtaa aaacaaatgt gatgggaact caaaatgtaa tagatgcagc
      421 gatcaaaaat aatgtaaaac attttgtagg tatcagtaca gataaagtag tgagtcctac
      481 taacaccatg ggtgcgacaa agttattgag tgaaaaatta ataatagctg ccaatcagta
      541 taaaggaaaa acagaaacaa tattttcttg tgtaaggttt ggaaatgtca tggggtcaag
      601 tggatctgtt attcctcttt ttagtaaaca aataaaaaac aataaagata ttactttgac
      661 agatgttgat atgacacgat ttatgatgtc aataccggat gctgcaagat tagttctgtc
      721 tgctgctatg aattcaaaat ttggagaaac atatgtctta aaaatgccgg caatcaatat
      781 tttaacttta gctcaatcat taataaaaat atataatgat acacatgatg aaaaatatag
      841 aggtgaaata aaagttgtag gtatcaggcc aggagaaaaa ttatatgaag agctaatgac
      901 tacagacgaa attgaacgct catatgaaaa tgataaaatg tatatcattc caccacacta
      961 tcatgctact gacgaactgt acaaagatta tgaaaaaatt aaaatcactg catactcttc
     1021 agatgcaaca gaattaatga gtggacagga atttattaga atcgcaaaag agaataatgt
     1081 cgtgacttgg taaggagttt tatgatgaaa tcagaagtat atgtaattgc agaaattggt
     1141 gtaaaccata atggaagtct agatttagct ttagaaagta ttgataaagc taaagaatgt
     1201 ggagctaacg cggttaaatt tcaaacattt aaaacgaata agctagtttc caaaaaggct
     1261 ccaatggcaa aatatcagaa ggataatctt aaagaggaca gttctcaatt tgaaatgcta
     1321 aaaaaattag aattatctca atctgatttt attagaataa aagattattg tatacaaaat
     1381 aaaattgatt ttttgtctac tccttttgat gaagaaagtg cagactttct aaaacaaatt
     1441 ggtgtacaag gatttaaaat tggctcagga gatttaacaa atttaccttt tcttagaaaa
     1501 atagatatgt atgggttgcc agttttatta tctacaggta tggctgatat tcatgaggtc
     1561 agtgaggctg tagattgttt catagatagt ccagttacta ttttacattg tacttcaaat
     1621 tatccggcta gttctgaaga tattaattta ttggcgatta acacattaaa agaaatgttc
     1681 gggatacccg ttgggtactc cgatcattca ttgggatatg atgttggtat ttgtgctgtt
     1741 gcgatgggtg ctaaggtaat tgagaaacat tttaccttgg ataaggaact acctggtcca
     1801 gatcataaag cttctttaga tcctcaggaa tttgcagatt ttgtaaagca tattagaaac
     1861 actgaaatat ttttaggtga tggtgttaag cgagcaatgc cttctgaact tagtacaaaa
     1921 gaagtatcaa gaaaaagcct agtggtcact gagaatttaa atgtgggaga tattcttaca
     1981 gagaaatctc ttacaataaa acgtcctgga aatggaatag ctcctaagtt tttatatcaa
     2041 tatattggca aaaaagttaa aagaccaata agtgcagata gtgttctgct tgaggaagat
     2101 gttgaatgaa aaaaatactt atagtaagtg gaacacgagc tgattatgga atatataaac
     2161 ctgttgctga gaatttacta caagataatt gggatgtatc atttgttgtt tctggaatgc
     2221 atttatccca taaatatgga tttactaaag aattgatctt agctgacgga tttagaatta
     2281 gtggagaagt tcaaagtttg tttttagaga acaataaggg aaacatggct cggtctgtgg
     2341 cattagagat aaatggtttc acaaatatat ttgaacaaac tagccctgat tttgtattat
     2401 tactagggga tagaggagaa atgttggcag ctgcgatagc ttgtttgtat cttggaatca
     2461 aaacagcaca tattcacgga ggcgaagtgt ctggtactgt agatgaatca ataagacatg
     2521 ccataacgaa aatagcatct gttcattttg ctgctacgga aaaaagcaaa gagcgtttaa
     2581 taaaaatggg ggaagattct tggagagttt ttcccgtcgg agctcctaga atagatacta
     2641 ttttaaataa tagattacct agttttagag atataaaaga aaaatatcat ctaaaatttg
     2701 aaaagaaaaa atacgttttg caagtatttc atccagttgt aaccgaacta gaaaatatag
     2761 aaaaacaaat tttagctttg atcaattcaa tggaacaaca atctcaacca attatttgca
     2821 ttttacctaa tagtgatgct ggtagcgaaa ttatcagaaa aatgtaccag aaatatttaa
     2881 aaacggatat tatttatatt acgaatttag agccggagga atacctgact atattaaaag
     2941 agtgcaaatt tatgatcgga aattcttctt ctgggataat tgaagctgca tcattcaaaa
     3001 taccagtaat aaatttagga accagacaaa atggacgaga acaatctaaa aatattataa
     3061 atgcaaatct aaatgaaaat gaaattgaaa ctgctttgaa
//
LOCUS       DACDSY010000211         3085 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDSY010000211 DACDSY010000000
VERSION     DACDSY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.214"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..2283)
                     /locus_tag="IWK44_002793"
     CDS             complement(<1..2283)
                     /locus_tag="IWK44_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551245.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPG"
     gene            complement(2955..>3085)
                     /locus_tag="IWK44_002794"
     CDS             complement(2955..>3085)
                     /locus_tag="IWK44_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS607 family transposase"
                     /protein_id="HAP9551246.1"
                     /translation="IDITENDEDLIQIVTICRYLLQGNRANKAKKMIKELMEDDTS"
BASE COUNT          842 a          704 c          510 g         1029 t
ORIGIN      
        1 tcctggtgct aaaccgtcaa ttgccagttt tccatcttct cctgtagtta aaccggtttg
       61 tagcgtttct ccttctcggt tctgtaactc aaaaacagct ccttgaagga cttcaccact
      121 gccttcagca tgtttaatta gttcaacacc acccgtatca aggatgtttt caatggttaa
      181 cgtcacttct tctatggagt attcatttat aaaaacatca tgtaccgtcg catctaaaag
      241 atacccttcc ggtgcttctg tttctcggac ttgatagcta cctacaggaa gcggttcact
      301 taaaaattct ccgttttctg ccgttatacc ttcttgtatc acttgtgaat cgcttgataa
      361 aatttcaaat tttgctcctg ccagcctttg gccattctca tcagttttaa ttactttaat
      421 acgcccatcg attttccttg ttcttacagt actcgtattc aatcgattgt cactattcac
      481 aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac gcctctacat tgaccttaaa
      541 agagagggta aaacttgtcg gaccaccaat cgtattattc gcactgaaac gaactattcc
      601 attattgact tcggcatcaa tgaaagaggg aatatctgat agaaatgaca caaattccat
      661 gccttcttcc aaaacatctt caataacaag cgtatttctt gttccagaaa acatcgagac
      721 atgcagagta tacgttagtt cattttcatt gagattgata acatttttgt ccacactttt
      781 tcttaaagag attgccggaa gattactttc agggattctc aagcggtagt ctcggctcat
      841 tacctgatta ttgtaggtca tactcgctcg attccgagaa aacccatcct cttgtcttcc
      901 ttcattgaac aagtcgacat cggtcacttc aagagcatat tcaataaaat acgattgacc
      961 ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt tccttattaa aagatacttt
     1021 gtccacaaat tcctcggaaa cataatgagt tctaacgttt gcttcacctg gattcgttac
     1081 ttctgcgatc cgaaccccac ctgaaatagg ttctctattt ccagtggaac tccatgtagt
     1141 cggattcggt gccaaagttg ttccttgcgg tagtacatcg gtaaccacta caccgtcttt
     1201 tcgtgatcct tcataattta cctgcaaaat aaatttatta ctgttggtca cgtcttgccc
     1261 gtcaatcagc agattcccaa gatttccttg gctgtcgatt tctctagagc catagtataa
     1321 tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt gaaaattccg tgtttgctgc
     1381 tcgctcagca taagtaagac tcacttgaaa gtcattattt ggctcagtac cgcctgattg
     1441 aaagaaagca acttcaaacg tgatactgaa tgtatacgac tgagcggctt catttaatcc
     1501 ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct tcttggtaaa atgccagtgg
     1561 ctttaaaggt gaatcatcag cgacctccac tctaatgaac tcaattaagc caatcgatgc
     1621 tctaggaact cgtacgacca tctccccata ttcttcaaga atatcgccag cgcctccttg
     1681 aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa gtggggcgct cagcagagat
     1741 gccgatgtca taattcaaga gctcctcttg tctttcaatc tgagattcct gcgttataga
     1801 tggctcagat gatctggctt cattaaaaga ttcacttaag ctttcttctg gtatattaaa
     1861 agattcactt gtgctttctt ctggtacatt aaaagactca cttgtgcttt cttctggtac
     1921 actgatttct attgagaccg tatttgattg aatatcaccg cttcttgcgg tcaattcgta
     1981 tttacctgaa tgctgggaag aataagtaat cagacgttcg taatcattga gttgcttcac
     2041 ttccccttca atcaaatcct cactcttatc aatgaccaat gcatttagca ggtctggtgt
     2101 tcctctgact cgcaactgga actgctcacc caaagcagcc tcctcttgat caacttcgat
     2161 cgtcagcgaa cgctctggct gttctgctaa agactcagct agaaccgtta tgttcccaaa
     2221 ttgattgatc acaataagca taactagaaa catacttaac agtttatgaa atttgttcct
     2281 cactattact cccccttata attttgacta aacaacaagg tacccgataa ttttctaaaa
     2341 agattcccca actcaaaatg gctgcgttac ctccctcgta ttttcggttc aactcaagcg
     2401 ttgcaatgta agctgtaaac aaagctaatg attgccgatt gtcttctgtt gattgattcg
     2461 atagctttgt atgactttta gttgtccgtg tggcttaacc ataattatga tttcacgatt
     2521 cctcctgcca gtgccttgca caagctggag cgtaacccta atagttattt tgttaatgta
     2581 cttatatact tttatacttt tccattagaa acaatcacta aatcgttgat accaaggtca
     2641 gactctagaa tttcatcagt gaatccctgt attggttaac cttcatcaat cccaaaaaaa
     2701 tatatcaatg cttagcatca aaatgaatat atagtttaga atacttggga ttcatcgata
     2761 actgatcgct tatttggcca tattatttgg cacttggttc aactaaacgc atttaggatg
     2821 ttccttcatt ctcgtcatgg atgttctcaa accgtcatta ctaataaact gtccaccgaa
     2881 tcgatggttc atttcttgca ttttccacgt ccaattactc tttttgctct ttatttgggc
     2941 gtagtttcac tttcttaact tgtatcgtct tccatcaact ccttaatcat cttcttagct
     3001 ttattggctc ggtttccttg taaaaggtat ctacaaatag tgacaatctg tattaaatct
     3061 tcatcatttt ctgtaatgtc aataa
//
LOCUS       DACDSY010000212         3040 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDSY010000212 DACDSY010000000
VERSION     DACDSY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.215"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(96..1868)
                     /locus_tag="IWK44_002795"
     CDS             complement(96..1868)
                     /locus_tag="IWK44_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9551247.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1822..>3040)
                     /locus_tag="IWK44_002796"
     CDS             complement(1822..>3040)
                     /locus_tag="IWK44_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9551248.1"
                     /translation="TNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFKALNKA
                     INDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVDLLMLF
                     FLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNCDIAFE
                     NVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQIKIGGK
                     PLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGCESILE
                     KFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEYELQKA
                     FQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQLVTNYE
                     NANDWRLDNEKFL"
BASE COUNT          993 a          565 c          429 g         1053 t
ORIGIN      
        1 attcaaaatt agatgctgtt taataaaatt attctttttg aaaaagaatt ataactatat
       61 cccaaaaatt gtaggttaat ttaaagccta tttcatcatc gtgattgagc ttctttttca
      121 tagacgaatt tctcagatag tttagttttc tttatgagat ttttgtatat ttctgacctt
      181 cgatacaact cattatgagt accttgatca actagttttc cttgatccat cactacaatt
      241 ttatttacct tttcaataga cttcaagcga tgcgatataa ttagtactgt cttatcttgt
      301 attaaacgat ttaaactttc ctgaatacgt ttttcatttt ccgcatctaa actggcagta
      361 atttcatcta aaattaaaat tggcgcattt ttcaaaaaag cccgtgctat ggataggcgt
      421 tgtcgctctc ctccagaaag tgaacttccg ttttctccga ccaatgtatg ataaccgtca
      481 ggtagttttt caataaagtc ttcgcagtta gctaaacgag ctgctttttt cacttcctca
      541 tctgacgctt gactatttcc aatacgtata ttttccaaaa tacttgtatt aaacaaagtt
      601 acctcttgaa aaacaatagc gattttgcta aacaaagaag ctggagatac acgtttaata
      661 tcatacccat caattaaaat acagccttca tcataatcat acagtctaga cactaatttc
      721 aagatactag ttttcccgga accactagca cctactaacg cagtcacttc tccttgttta
      781 gccgtaaaag agatatgatc taaaattggg gtattgttat catatgagaa agagacatct
      841 cgcaactcaa tgtcaaaaga tttcagagga ctatctgatc cctcttggat actggtttcc
      901 ttcatagctc taattcgctg tattttagga gctaaataaa atatttcaag cactgcttct
      961 ttcatagaat cgaaggaatc ctttattttt attgcggcta gtaagtaacc aacgacgtag
     1021 agtatagtca cttcacccgt taacagtaag tgaacaccta ctagaatgac tactgctaaa
     1081 ctaacatatg agaaaataga ggataaggcc atgactgaaa aagtacttaa ttctaccttt
     1141 aaatgaatcc tttcactctc ttccattttt ttgaaaagtc tgtcttgaac ttttttagat
     1201 agattaaagc tattaatctc ttgctgcaat tctatagttt cttgaaattc ttccgagttt
     1261 tctctcaaag tatcgtaata tttagtattg gctttcgttt gggatttctt tgataacaag
     1321 attaagacaa aactaaataa cgttggcaaa ataacagcta atcccatttt gacattacca
     1381 accaaaagca tcactgaaat aaaagggaaa aacagagcca taccgccggc tttaggtatt
     1441 gcatgactca tcgcatgctc aataccttca acgtcagaca tgatagtttg agataaatct
     1501 gataaattat gttttgaaaa ataggataat ggtaaatttg acaaatcgtc tgcaatttgc
     1561 actcttaaat gcgcactttc tttataagta ctattatata agttttcata ctccctatct
     1621 aacaaccaat acatgaaaag caaagtaaca aacgatacgg caaaataaag ccaacggggc
     1681 tttgtatttt ctaaaactaa ttcgtctatc agcatcatta taatgaatgc tggtaccata
     1741 ttaatgcagt atacaaaaaa actagaaata gaagctttag ttaaagcttc tgcgccctga
     1801 tcacttaaag caaatttgtt tttataaaaa cttttcattg tcaagcctcc aatcatttgc
     1861 attctcataa ttagtgacca attgtttata cacttgatgt ttagccatta attcattatg
     1921 atttccacgt tctactattt tcccttctga caagacaaga atttcatcta cagatttaat
     1981 gctggataat cgatgagcta tcataataac tgttttttct ttcatcagat gttgaaaagc
     2041 cttttgtagt tcatactcat tatctggatc aatagatgca ctggcttcat ccataataac
     2101 aatctttgca tcttttaaaa ttgctctggc aatagctata cgttgttttt ctcctcctga
     2161 cagataaact cctttactac caattaaggt atgctctctt tcgggaaatt tttctaaaat
     2221 cgattcgcaa ccggctaatc ttagcgcgtc aaatacttta cttgctggcg cttgaggatt
     2281 tgctatagaa acgttatcgt aaatactctg attgaatagt ttcgtatctt gaaagacaaa
     2341 agcaattgtg ttaataattg tgtcttttga atagtgattt aaaggcttac caccaatttt
     2401 aatctgtccc ttatccacat tgtagaaacc tgaaattagt ttagctatcg tactctttcc
     2461 gcttcctgat tttcctacta atgcataaat tcttttttgt tctaaaacta agttgaaatt
     2521 ttctaaaaca cactcttctt tgtatgaaaa gctgacattt tcaaatgcaa tgtcacaatt
     2581 tttaatctgt gtctcgttcc catattttag ttggtcttgc ttcatttcta aataaaggga
     2641 gtctagtgtt tcgactgcat atcttgcttc aaacaagtgc atacttacat acattattct
     2701 catgaaagaa acaaaaataa ttccacttaa aaaaaacaac atcaacaagt caacagctaa
     2761 tatcttggga gtggtaagag aagttataaa aaaggttata ggaactatca aaatgcaaac
     2821 tatgccaaaa aatagccatt gaaaaagaac aaaaggtgtt ttcccactca aagaatactt
     2881 atatgcaaac ttagcataat cgttaattgc tttattcaag gctttaaatg atgttacttt
     2941 agcaccaaat atctttataa cttgaattcc gcggatatat tctaccgttt cagagcttaa
     3001 cacatctaac gattcttgat atttttgaat aaaatttgta
//
LOCUS       DACDSY010000213         3033 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDSY010000213 DACDSY010000000
VERSION     DACDSY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.216"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            74..1585
                     /gene="gadC"
                     /locus_tag="IWK44_002797"
     CDS             74..1585
                     /gene="gadC"
                     /locus_tag="IWK44_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAP9551249.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            1598..2998
                     /locus_tag="IWK44_002798"
     CDS             1598..2998
                     /locus_tag="IWK44_002798"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP9551250.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
BASE COUNT          927 a          499 c          612 g          995 t
ORIGIN      
        1 tgcaagctca aataagcgat tttccataac cttggtagga atgacaaatt aaataggaaa
       61 gggaagtaat aatatgaata aaaaaaagtt atctctattc ggtttttttg ccctgacagc
      121 ttccatggtt ttgactgttt atgagtatcc gacctttgca acatcacaat tacatttggt
      181 tttctttctt cttttgggag gtattttatg gtttctacca gttgctttat gtgctgcaga
      241 aatggcaaca gttgaaggat ggaaagatgg tgggattttc agttgggtta gtcaaacttt
      301 aggagaacgt tttggttttg cagctatttt tttccaatgg tttcaaatta cagtaggttt
      361 tattacaatg atctacttca tactaggagc gctgtcgtat gtaattaatt ttccagagct
      421 taatacagat ccatttatca agtttattgg tgtattagtc attttctggg tactgacttt
      481 ttcacagttg ggcgggacca atcgtaccgc taaaattgct aaagcaggat ttgtcattgg
      541 aattgttatt ccttcgatca ttctctttgg tttagcagca gcctacttta tcggcggtaa
      601 tccgattcaa atggctatta atgcgaaagc ttttattcct gacttttctc agctttcaac
      661 tttagtagta tttgtatctt ttatattggc ttatatgggc gtagaagctt cagcatcaca
      721 tattaatgag ctggaaaatc cccaaagaaa ctacccattg gcaatgtttt tactagtgat
      781 tttggccatc gccttagatg caatcggcgg tttctcagta gcagcggtta ttccacaaca
      841 tgacttgtct ctcagtgctg gtgtaatcca aacatttgaa acgcttattt tacattttaa
      901 cagtcattta cattggttgg taaaagtgat tgctctcatg attgcctgtg gcgttatggg
      961 tgaagttagt tcttgggtag ttggtccatc acgagggatg tttgcagctg ctcagcgagg
     1021 tctgttacca gttttcttcc ggaaaactaa taaacaagac gttccagttc cgttgattat
     1081 gatacaagga attatcgtta ctatttgggc agcagtctta acttttggtg gaggaggcaa
     1141 caatttatca ttcttagtag caatctcctt aactgttgtc atttatctcg taggatattt
     1201 actatttttc ctaggttact ttgtattagt ttttaagaaa aaagacttaa aacgaactta
     1261 caatgtacca ggaaaaactg ttggcaaaac gattatcggt ggaattggtt ttattttatc
     1321 agtgttcgca ttgcttatct cttttgtacc accagcttca attgcagcta gtgagcaccg
     1381 aacataccaa atcatactac ttattagctt tgtagtgact gttcttttac cttttatcat
     1441 ttatgaagta catgataaaa gtgctcacac aactattgag gaacctactc acgtgaaagc
     1501 tgaagacgtt aatcccgctg tttatccagc tgctagagga gagcaccacg ttacaaaaaa
     1561 tgatgaacat attttaaaac attaggagga attcattatg ttatacggaa aagataatca
     1621 agaagaaaaa aactatttgg aaccaatttt tggctctgca agtgaggatg ttgacttgcc
     1681 taaatataag ttaaacaaag aatccattga accacgaatt gcttatcaat tagtacaaga
     1741 cgagatgttg gatgaaggaa atgcgcgatt aaacttagct actttttgtc aaacgtatat
     1801 ggaacctgaa gcagtgaaat tgatgaccca aacgttagaa aaaaatgcaa ttgataaatc
     1861 agaataccca cgaacaacgg aaattgaaaa ccgctgtgta aatatgattg ctgatttatg
     1921 gcatgctcca aataatgaaa aattcatggg aacttcaacg atcggctctt cagaagcctg
     1981 catgctgggt ggtatggcca tgaaatttgc ttggcgtaaa cgtgctgaaa aattaggtct
     2041 tgatattcaa gcaaaaaaac ctaacctggt gatctcttct ggttaccaag tttgttggga
     2101 aaaattctgt gtatattggg atgtggaact gagagaagtc ccaatggatg aaaaacatat
     2161 gtcaattaat ctagatactg tcatggatta tgtggatgag tacacaattg gtattgtagg
     2221 tattatgggt attacttaca ctggtcgtta tgatgatatc aagggtctga atgatttagt
     2281 tgaagctcac aataaacaaa ctgactataa agtatacatt catgttgacg ctgcatcggg
     2341 tggcttttat gcaccattta ctgaacctga tctagtttgg gattttcaat tgaaaaatgt
     2401 tatctcaatt aattcttcag gtcacaaata tggtttggta tatccaggtg tgggttgggt
     2461 cttatggcgt gaccaacaat acttaccaga agaattagta tttaaagtta gttacttagg
     2521 tggagaaatg ccaactatgg ctatcaactt ctctcatagt gcagcacaac taattgggca
     2581 atactacaac tttgtacgct atggctttga tggttatcgt gatattcacc aaagaactca
     2641 tgatgttgct gtttatttag ccaaagagat cgaaaaaact ggtatttttg aaatcattaa
     2701 tgatggatca gaattgccag ttgtgtgcta taagctgaaa gaagatccca atcgcgaatg
     2761 gacactatat gatttatctg atcgtctgtt aatgaaggga tggcaagtcc cagcctaccc
     2821 attacctaaa gacttggatc aattaattat tcaacgctta gttgttcgag cagactttgg
     2881 aatgaacatg gctggtgatt atgtacaaga tatgaaccaa gcaattgaag agttgaataa
     2941 agctcatatt gtttatcata aaaaacagga tgtgaaaacg tacggcttta ctcactaaaa
     3001 ttcttcagag aaatataaaa gaagttaatt aac
//
LOCUS       DACDSY010000214         3013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDSY010000214 DACDSY010000000
VERSION     DACDSY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.217"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            177..359
                     /locus_tag="IWK44_002799"
     CDS             177..359
                     /locus_tag="IWK44_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAP9551251.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="IWK44_002800"
     CDS             462..746
                     /locus_tag="IWK44_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551252.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="IWK44_002801"
     CDS             762..998
                     /locus_tag="IWK44_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551253.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="IWK44_002802"
     CDS             1020..1274
                     /locus_tag="IWK44_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551254.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="IWK44_002803"
     CDS             1356..1541
                     /locus_tag="IWK44_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP9551255.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="IWK44_002804"
     CDS             1608..2003
                     /locus_tag="IWK44_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP9551256.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="IWK44_002805"
     CDS             1993..2370
                     /locus_tag="IWK44_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551257.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2371..2766
                     /locus_tag="IWK44_002806"
     CDS             2371..2766
                     /locus_tag="IWK44_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551258.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          925 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc ttt
//
LOCUS       DACDSY010000215         3009 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDSY010000215 DACDSY010000000
VERSION     DACDSY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.218"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            199..624
                     /locus_tag="IWK44_002807"
     CDS             199..624
                     /locus_tag="IWK44_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551259.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(1112..1813)
                     /locus_tag="IWK44_002808"
     CDS             complement(1112..1813)
                     /locus_tag="IWK44_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9551260.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(1829..2269)
                     /locus_tag="IWK44_002809"
     CDS             complement(1829..2269)
                     /locus_tag="IWK44_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9551261.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(2269..2844)
                     /locus_tag="IWK44_002810"
     CDS             complement(2269..2844)
                     /locus_tag="IWK44_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP9551262.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          933 a          585 c          477 g         1014 t
ORIGIN      
        1 ttaatatagc atttttttaa aagattgcaa cactttttta aaacgctttc tgaaagtggc
       61 ggattttttg cctttttaaa attttattct aaaatttact ctatttgtgg gtgttttgtg
      121 cgtttttctc ttccatccgt ttgaagtttt acattccaat ggtataattt ctttatccta
      181 tcaaatgggg gaagtatttt gaagaaatta atcgtactat ttattttgtc tgcgggactt
      241 ttgacattga ctggctgtga tttgtttggc ggctctgacc aatcctcgga cgaaacaacc
      301 gattcttctg tcttaaaagc ggaaaaagca gataccacca acagtgtata tacgctaaaa
      361 gtacgtaaag atatggattt atccgcggga cagttgacat ttacagataa aaaaatggaa
      421 tggacacgca cgtatcagac acaaggaact gattcttctg aatcatctga agaaaaaaca
      481 gtgctgacag atatcaagat cactaccaaa gatgacgaat atattatttc aggaaaagaa
      541 aataaaaaag aaacaacagt catatttaca aaaatcggta attaccgttt gaaggatgag
      601 gatggcaaca tctacagtct atagtgccgt ctgttggatc ttcttttgtt tctccccttc
      661 ttttctgcta tagtaaagaa gcaagatgat tgtaaaggag acgaaatgga tgatcgatag
      721 tcctgattat tgtacatgta ataacaacaa aaatatccat acagaattta atgaccaagg
      781 aagcttgagc atatgcgaca actgtcacaa actagtcaaa gattcattta aaacatttga
      841 atcttatcct gttcaaaatt atgtaaaaag tagaagaaga taaacaaatc caagcaaaac
      901 tcactatggc tgatttttat gaacttcaaa ctcagcttct tcctttgatc aagcttcatc
      961 agtaaaacaa acagtggatc atttcagttt acaaatcaaa gagtaaagat ttatttattt
     1021 caaatatttg aatataaaat aaccaaacga tagaaaggct tattctacat cacgtagtaa
     1081 tttttctttc ccgtcgtttg gttttttatt tttagcctaa ttcttgacct tttgcatgct
     1141 ggttatccgt aaaatatttt ccagcaggct ttccttccaa ttcagctaat ttttcttgga
     1201 tctgacggat cacctttttt gcatcttctg gattttctac aaaatcgaat ttatccccat
     1261 ccacttgtat tttaggacat acatcaaaat cttcatacca ttgattatag cgggtgttca
     1321 acgtttcata ataggtatat aacgtctcat cgctttctag ctgttcatag tcacgcccac
     1381 gttttttgat acgttctagc atcgtgtcca aagaaacttt gatatggacc atcaaatcag
     1441 gccgtttttt tggagcaaca tcatctaact cgtttagcat cgtatctaat aagctatcgt
     1501 actgctgtac ttcgatatcc gttacccgac ctaaatcagc gtttagatgg aacaacaggc
     1561 tatcttcata gatggatcgg tctagcacat tgtcatcgtt cactaaagca tttttcattg
     1621 ctaaaaatcg cttgttcaaa aagaagattt gcaataaaaa agcgtactgc tcaggatttg
     1681 aataaaagag cggcaatact tcgttatctt cgacattttc ataaaatggt ttagaatcaa
     1741 tctcttttgc aagcatatcc gtcaaactgc tcttacctgc accaatcgtt cctgaaatca
     1801 aaatcactga catacaaccc ttcctcttct ataagtactc gcctacgtaa gtttcaacag
     1861 gcaatgtttc aaaatcatat tcacgtacag cttggtctga ttttaaatac gtatggagac
     1921 tttgtgtaat catcaagttc gtaggtaccg tctcttcttg tacaatgatc atcggttttt
     1981 tcaacatagt tgccacacct aattcgtaag ccgttcctgg atcgattgtt tgatgctcaa
     2041 agtcaaggat agcaacaaca aattctgcat ttgtcagttc ttccatatct ttttcgtata
     2101 cttcctgcgc ccatccagcg ctgaatagtt cataattgct ttcttggtga tgacgtggag
     2161 agtagaaact tgtgactgtt ttgttttcct ccaacgcttt ttcgatacga cttactcgat
     2221 cgatttgctc ctctgaaaaa aatggtcctg ctaaatatat ttgactcatt attttgcctc
     2281 cttcaataat aactgatttt taatagatac tgctcgtaat tcattgacca gttccaatgg
     2341 attgttggaa aagattactt gatccaacca ttttttatcc ataaaatcct cttttgcaaa
     2401 agtttctaat aattgttgca agccgctgta ataaccgcca atattcaata ttccgattgg
     2461 cttgtcgggc gtcagaccga ttgctatcca agaaagaagt tgtgagaatt cctccaatgt
     2521 acctagacct ccagggaaaa cgaggacagc atctcccttt tcaaataata atacctttct
     2581 ttcatcgatc gtttctgtag gaataaatgt agtaacatct tttccaggca attcagtttc
     2641 aaaaagccca ttcggataga tacctgtgac ttttccgtta ttttccagta ctgttttact
     2701 aatcactccc ataatcccgg agtatgaacc accgtaaacc agttcgattt cttcttgtgc
     2761 catgtatttt cctaacgttt gcgcaataaa tttgtaactt tctttattac caaaacggga
     2821 accgcaaaaa acagtaagtt tcataaaaaa acactcccta actcaaaagt ctatcttcat
     2881 tctacacgtg tttttttaaa ataaaagact agattttgta gttttttagt ggtatatagg
     2941 atagacgcca ctatatatag tatttagaaa aaaatcattt ttcagccatt ttatagataa
     3001 aacaaaaat
//
LOCUS       DACDSY010000216         2947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDSY010000216 DACDSY010000000
VERSION     DACDSY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.219"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            39..503
                     /locus_tag="IWK44_002811"
     CDS             39..503
                     /locus_tag="IWK44_002811"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP9551263.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(565..1608)
                     /locus_tag="IWK44_002812"
     CDS             complement(565..1608)
                     /locus_tag="IWK44_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP9551264.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1985..2938)
                     /locus_tag="IWK44_002813"
     CDS             complement(1985..2938)
                     /locus_tag="IWK44_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP9551265.1"
                     /translation="MKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMILMIF
                     IFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWLCAVI
                     FQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFFWISL
                     ICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLVVALF
                     ALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYSSVLD
                     FLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         1043 a          547 c          583 g          774 t
ORIGIN      
        1 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
       61 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
      121 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
      181 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
      241 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
      301 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
      361 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
      421 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
      481 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
      541 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
      601 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
      661 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
      721 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
      781 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
      841 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
      901 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
      961 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     1021 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     1081 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     1141 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     1201 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     1261 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     1321 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     1381 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     1441 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     1501 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     1561 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     1621 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     1681 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt
     1741 tccagaagta gcttctacgg aaataagccg aaatttcaca aaaatttgag aagcaatttt
     1801 cgtaaattcc ttcttatttc ttgaagctga ccacttcttt tacgacctct tggtaaacgg
     1861 tgttcaaaag cttgcatctg cggtaataag cccaaccagt acaaaaacat gaacagctgt
     1921 ttttgtttgg ccgttcttat tacttgatgc agaacagctt tttcacaacc tattgaatcg
     1981 attattaagc gattttttta gaaacaaatt ttccatgttt gctatgttct ttaattaaga
     2041 aacgtacgta atgagcaaga acgtagaaga caactacact tgcaaacaga cattccgcaa
     2101 cttttaagaa atccaatacg ctactataga tacctaaact gttgaagtaa tgaacagtag
     2161 taaatatagc tgttgcagag ataacaagcg ggaatgtaaa ggctgcatag cttggataaa
     2221 aacgaagctt gaacaaacgt ggaagcatag acagcacaac gaaatacaat acttgagata
     2281 aagcgaacaa tgctacgaca agataaaaat taggttcagc aaagttttta agataacctg
     2341 ttaggcaaag agaaccaggt gctgcaacga tcgtaatcag cggacgtgtc ggatgagcca
     2401 tatttcttac gaaaaagaca cgtttgatga tgatcggcaa taagaacgca tagcagatca
     2461 atgaaatcca aaagaaaatt tttccaataa gtgggaagaa gttcccggca gttacagtga
     2521 tgaccccaaa tccaacgtaa atgataaacc agcttggata aattgcttca attgaaagat
     2581 cagctttgat aacatggtga taattgaaat aaaagacaag gatcacttgg aagataacag
     2641 cgcacagcca aatgaatttg acaattggtg caatactttc atatgaaaca aaataagtac
     2701 aaataaccat gagtgacatc gtaaaagttg gtgaaacgga ggcgatgatt ggatcatgca
     2761 acgtttgttt cgtgtgttcg aataagataa acaatttaac aaaaatgaat atcataagaa
     2821 tcatagagat accgccagtt agattgccta aagcaacatg gtgatagtct ttaaacagat
     2881 ttccaaggga agctaaccca aggatcaacc cgcagatcgg gatcggaata attttcaaaa
     2941 agttctt
//
LOCUS       DACDSY010000217         2940 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDSY010000217 DACDSY010000000
VERSION     DACDSY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.220"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(65..1285)
                     /locus_tag="IWK44_002814"
     CDS             complement(65..1285)
                     /locus_tag="IWK44_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9551266.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1910)
                     /locus_tag="IWK44_002815"
     CDS             complement(1305..1910)
                     /locus_tag="IWK44_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9551267.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="IWK44_002816"
     CDS             complement(1959..2891)
                     /locus_tag="IWK44_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9551268.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
BASE COUNT          809 a          616 c          513 g         1002 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
//
LOCUS       DACDSY010000218         2940 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDSY010000218 DACDSY010000000
VERSION     DACDSY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.221"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            181..537
                     /locus_tag="IWK44_002817"
     CDS             181..537
                     /locus_tag="IWK44_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9551269.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            547..1446
                     /locus_tag="IWK44_002818"
     CDS             547..1446
                     /locus_tag="IWK44_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP9551270.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1843..2922)
                     /locus_tag="IWK44_002819"
     CDS             complement(1843..2922)
                     /locus_tag="IWK44_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9551271.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          793 a          651 c          686 g          810 t
ORIGIN      
        1 tttgggtctt tttccgttca agaaactgaa cgaagaaaag ggaaagttgt ctcttttgtg
       61 ataagagaaa tcataataaa cgaatgactt actttccaat aaattgagaa cagtcagaac
      121 cagacaaatt agttgcatct gatgaataaa gtgattattc tatgacatga ggtgatacag
      181 atgaaattag aagtgacaaa agaagcacaa gaagtattga agaataaatt agaagcagac
      241 gatcaacttt tattagatat cgaaaacggt gacggtccat ttgcagaaag ccaagtcagt
      301 tgccaattgg atacggcatt tcgattgatc attgtcaaaa aagatacaca aacagatcta
      361 agtctttatt ctgtagtggc tgatacacca gttggaccaa tcaaaatcaa agacagtgca
      421 atactttaca tggatgatcc atccacactt gctttagaac caacttataa tagtttacaa
      481 ttaaaaggac caagcggctt gagaaaaggc aacttgcaag ttgttcgaaa agactagagg
      541 gataaaatgg gaaaattggc gatcatcagc gacctacatg tggacatcaa taaattcggc
      601 gaatcagagc tgatgcagct ttggcaagtt ttgcaaaagc acaagatcac tcggcttcat
      661 ctagccggag atacggctaa taaagtagac cgttgcctag ctgttgtcga atttttcgtc
      721 aataaaggat tatctacgac atttcatttc ggcaatcacg agttggcaga tgtgcccaat
      781 gaacagatga tggaccattt tccagaccca cattttttaa atgaacggta tattgcttta
      841 aatgagcaga ccgttctttt agggatgaat ggctggtacg actatcagtt ttccgaattg
      901 aaagattacg aggcaatcgt acgaatgaag cgtttgtatt ggtatgatcg gttcatccag
      961 cgtgggaaaa cagatctcga ggtcaatgca gaaatgcttg cagctgcaaa aaatttgcta
     1021 gaccagctgg aagcaaaaaa acttgaggtg attttatcca ctcatttcgt tcctaaacgg
     1081 gcatttatca tgtatcaatc tgctccttat gagcgttgga acaaattaaa tgcattttta
     1141 ggatcagaat cattcggaga attacttgac cgttattcaa atatcaaaca agtagttttt
     1201 ggccatacgc atcgtcgctt tgaagataaa tccatcggtc acacgattta cagctgcaga
     1261 ccttttggtt attattatga atggcagctg acccgagatt ttgtcttgaa agaacagttg
     1321 ctagacagct atgatcctat gaaattacgt gtcctcttgc gtgcccatca cccagctttt
     1381 caggcctatc aaacacaaca tttgcaagag gaatttgaac aagcgatgac aatcgttccg
     1441 tattgagaca gcattagtaa tgtgtaaaag aaaatacctt gtaaaacata aggataagta
     1501 agagcagtag atccatttat cgtggatatg ctgctttttt tgcggaaaat aatcagcgaa
     1561 tgtttatttg atcggatgat ttgtaactcg cagagtacta gatgcaagcg tgtattcttt
     1621 ttttgaacga taatcacaaa aaaaaatata atcgtaaatt tcaagtttaa gttagccacc
     1681 catggtgacc cggttgctcg ttaccaccaa ccgtctaatt tttctaatcg agtgggccct
     1741 tgacaatgag ctgaggcatg gtagtctccc gactggctgg ccccggccag ctttccagac
     1801 acaagccatg ccgagggagg ctcgttgtca agggcccacg tgtcaagccg tcaaccgttc
     1861 ctgcctaaat gcctggacga acgtgtcatg cggcgttgca tagcccaaaa tctttcgcgg
     1921 gtaatcgttc atccaacgct gaatccgcaa gatttggttt tcgctcacgt tgccgattgg
     1981 gctgcccttg gggatgaaac ggcgaatcag cttgtgttgg ttttcgctcg tcccgcgctc
     2041 ccaggaagca taggggtggc tgaagtagat gtttgccaca tcctgcaagg catcgtgcag
     2101 tcccgcgaac tccgagccgt tgtccgaggt aaccgtcttg aacagctggc cgaagtacgc
     2161 accagcccgt tctctcagct gtcggaccgc ttggttgacc gattcggcat ccttcccggc
     2221 cagcttcaga atgacctcga agcgtgtctg ccgttccacc aacgtcagca gcacggcatc
     2281 cgacttggtc ttgtttccta cgaccgtgtc gacttcccaa tggccgaagg ttttccgggt
     2341 accaaccgcc tccgggcgct cttcaatgga tttccctagc acccgtttat tcgggcggtt
     2401 gccatgacgg gacgatttcg gtttgcggga cagtttttct agcaggtcca tattcttcgt
     2461 tttcatgatg ccacggtcga tccactgata gagggtggtg gtcccaggga taatggccgg
     2521 gtcgaacaat tcgtgttttc tggcaaagcc caccaccacg tccggggacc atttttccaa
     2581 cagcatcatg tcgtccgccc attcaatgaa ggcgtccgtg tcggcccata agggccgtct
     2641 gccgcagttc aagcggttct tctcgtaggc cgcctgcccg gcatcggcgt cgtagacgtc
     2701 atgataatag tcgtagacct tcccgttctg attctggcgg ttaagctgag taacggtccc
     2761 gcgtttgatt tcgttgttta tggtctgcgg cacgcagccc aggacatccg caacctgacg
     2821 gttggaatag ttttcctttt tcaggatggc gatttggcaa cgttctgagt aggttaggtg
     2881 ttttcccttg cgtgaaactg tgttatcatg gttttgcgtc atgtgaattc atccttgtct
//
LOCUS       DACDSY010000219         2849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDSY010000219 DACDSY010000000
VERSION     DACDSY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.222"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            802..1068
                     /locus_tag="IWK44_002820"
     CDS             802..1068
                     /locus_tag="IWK44_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551272.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1126..1638
                     /locus_tag="IWK44_002821"
     CDS             1126..1638
                     /locus_tag="IWK44_002821"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9551273.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1635..2672
                     /locus_tag="IWK44_002822"
     CDS             1635..2672
                     /locus_tag="IWK44_002822"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9551274.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1029 a          502 c          568 g          750 t
ORIGIN      
        1 tttattgatg accacgaaag tgcatggcga tatttgatgt tgatacttta tgcagtaccc
       61 tatgtattct tcgctatcat tctgtggctc atagcattgt tttttagagt atttaatgac
      121 ctttaggtag atttatatat aaaaagggct aaatacgcaa aataccccct taaaatgcaa
      181 aataaatgca aataaggggg taactcctta tgaaaaaaca actaatagta ttataacacg
      241 tttttatgcc gagaaaactt attggttgga atgggctatg tgttagctaa cttgttagcg
      301 agttggttgg acttgaattg ggattaatcc caagaaagta ccaactcaac aacacataaa
      361 gccctgtagg ttccgaccaa taaggaaatt ggaataaagc aacgaaagga gttgagtaaa
      421 atgagtgaga aaacgaagaa aatcattcga gtatcattag ctttttcaca agtagtagtg
      481 caatcattga tagtttatta tacttaccag caatataaat tatctaaaga gaattaagtt
      541 ttgattatgt aataagtttt ggtactaaaa aagacttgat ctaattagac caagtctttt
      601 gttagtgtta tattaataac aaaataaaaa taagaagtcg ctcactcccc gaccaaagtt
      661 tgtgagcaaa ataaacagtc gatgtttgtt tcttaattgt atatcttgat attaaacgat
      721 ttaagattat tcttcaagat atatattcgc aaatgtaatt ggaaaagcga cttcttaaac
      781 aaaattaagg agtcgctttt tatggcaaaa ataacgaaaa atgagcaaga aaatttggtg
      841 gaaaattaca caaaaaaacc ggcgtactct aatagccggt tagacgcaca tttcgtgtgc
      901 acttctaatc cgaaattgag ctttgacgct atgacgatag tcggaaacct taataaaaac
      961 agcgctaaaa agctatccga ttttatgagt ttagatccac aaattagact ttgggatata
     1021 cttcaaacaa aatttaaagc taaagcacta caagaccccc tatgttaggc tagttgtcgt
     1081 ataggataat aagttataat aaagactata cggaggatga ttaccatgac aaataaaaac
     1141 aatgaacgtt actctaccga actgaaagcg acagccatca agaaaatgat gccacccgaa
     1201 aataggggga ttcgccaatt aagtcaaact ttgggcattc cagaagcgac tctttattct
     1261 tggcgtaaaa aagctagaat tcaagggaat actacacccg gaacgaaaaa aaatgtcgaa
     1321 aaatggtcaa gtgaagataa atttttaatt gtgatggaat cattctcaat gaaccaagta
     1381 gaacttgcgg aatattgccg aaagaagggc ttatttaaag aacaaattga ggcttggaag
     1441 aaaacgtgct tatctgcgaa tgaacaggaa gaaaatcgaa cgagagaact tgcaactgaa
     1501 ctaaaagaag agaaaaaaca agcgcgtcaa ttagagaaag aattacgtca taaggaaaaa
     1561 gcattagcgg aagccgctgc tctcttatta ctaagaaaaa aagcacacgc gatttggggg
     1621 gaccaagagg aagaatgatt tcatcttcag atcgcaaaca agctgttgaa cttattcaag
     1681 aagccaatcg caatggggcc cgtctcacct atgcctgtaa tgagctgaat attagcgttc
     1741 gaacctacga acgatggacg cgtgaaggta cgatagcgca tgatcaacgc cctttggcga
     1801 aacgtcctgt acccaaaaat aaactgacgg atcaagaacg cgaaaaaata atagaaaccg
     1861 tttcaaaaaa agaatttatg aatttgccac ctagccaaat tgtaccaaaa cttgcggatt
     1921 gctcgattta tattgcatca gagtctagct tctatcgtgt attgcgagaa aaaaatatgc
     1981 aacatcaccg tggccgaagt caagtttcac aaaaacgcat accgccaagt caccttgcga
     2041 cgaagccaaa tgaagtttgg acttgggata ttacttggct aaaaggtcca ataaaaggac
     2101 tcttttaccg gttatatctc atcattgatt tatttagccg taaaatggta ggctgggaga
     2161 tttgggaatc agaagaagct cgtcatgccg aaaaacttat taaacagacg gtgcttaagg
     2221 aacgtatcca aggacagccg ttggttttac actccgataa tggcagccct atgaaagccg
     2281 cgacttttca agtcttactc gaaaaattag gaattcaaag ttcttattca agaccacgag
     2341 tgagtaatga caatccttat tcggaagcgg ctttccgaac gttaaaatat cgaccagaat
     2401 atcccaataa cggctttaaa acaatagatg atgctcgaaa atggacgaaa gagtttgtcg
     2461 attggtataa cgattctcat ttgcatagtg ggattcaatt tgtgtctcct gcagcgtgtc
     2521 atgaaggacg acatctcgct attttagaga aacgaaagca ggtttacgag gacgctaaaa
     2581 agcgccatcc tgaacgctgg tcaaaaggtg ttcgaaattg gactccacat caatcggtgg
     2641 cgttgaatcc tttaaaagaa acagaaagtt aacaggagca taatctttca agattatcaa
     2701 ataaccctcg tcacccaata ggggaaaaag ttattcttcc cctattggat aacgaggcca
     2761 accgaccata gggaggttct gctttttgat aggtggaaaa taaaaatacg acaactatat
     2821 tgacaaacac cgaccatcgt ccagtaaag
//
LOCUS       DACDSY010000220         2712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDSY010000220 DACDSY010000000
VERSION     DACDSY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.223"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..375
                     /locus_tag="IWK44_002823"
     CDS             <1..375
                     /locus_tag="IWK44_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551275.1"
                     /translation="EWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVDYFANTP
                     EEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEYNYIV
                     KAKVSKRFIFNPKLEIYSVVIE"
     gene            700..1317
                     /locus_tag="IWK44_002824"
     CDS             700..1317
                     /locus_tag="IWK44_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9551276.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1358..1657
                     /locus_tag="IWK44_002825"
     CDS             1358..1657
                     /locus_tag="IWK44_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551277.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1691..1858
                     /locus_tag="IWK44_002826"
     CDS             1691..1858
                     /locus_tag="IWK44_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9551278.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1903..2508)
                     /locus_tag="IWK44_002827"
     CDS             complement(1903..2508)
                     /locus_tag="IWK44_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9551279.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          949 a          409 c          495 g          859 t
ORIGIN      
        1 gaatggaata atctgactca aaataataaa aataattctg gatgtggaat tatctttatg
       61 tttgttatag ttttgggaat agtagtactt ttaataactg tcaagatagt agattacttt
      121 gcgaatactc ctgaagaaca aatacagaga aaacttcctg agtttgaaag agtaccacct
      181 tatgatccaa gagataattc atttcaagta aaaaaattaa aagagtctcc aaatgaaatg
      241 tggtttgaag ttacagttga atcaagcgac ttcaaagaaa aaaagaaaga atacaattat
      301 attgtaaagg caaaagtatc aaaaagattt atttttaacc ctaaattaga aatatatagt
      361 gttgtaatag aataatagtg ttaaaaaaat tgtaaaagtt aaagaagtca aattctgatg
      421 tgaaaaataa aatataccat ggaagacata aaagaaaaac tggataacta gtttatttaa
      481 tggcagaccg ttaaggcctg ccatttttat gtattttagc ggaataaata taatcatgcg
      541 ggagtgtaaa cttactgttt caaaaagtat actttttgac atgccttgat tttctttgtt
      601 tatgtatggc ataataacca cataatacaa tttgaaacgt ttctgattta atctgattgt
      661 tttgaaacaa ttttagtgaa aaaagggtag ataaaaacga tgagaaaatt tttttatatc
      721 cgggtcagta gttatgatca atcgatcgaa cgacaactga tttccgcgaa ggaattagga
      781 atccctgaag aatatatatt tgtagaaaaa gccagcggca aagattttaa acgccctgaa
      841 tatcaattaa tgaaacggat gtttcgaaaa ggagatttac tctatctaca atcattagat
      901 agactaggca gaaataaaca aatgatttta gaagaatggc gagaattaac taaggaaaag
      961 cagattgata tttctgtgtt agacatgcct ctgttagata ctgctagtta caagcaacta
     1021 gatgggttag agacgcttat ctctgactta attctgcaat tactcagcta tatggccgaa
     1081 gacgagcgga aacgaattaa tagccggcaa agagaaggcg tagaagctgc gaaacaaaag
     1141 ggtgtaaaat tcggcagaaa gaaaattgct ttacctcctg aatttcctgc gatctattct
     1201 gactggaaag caggaaagat aactgcagtc tatgctatgg atcaattggg catgaaacga
     1261 aatacctttt atcgacgggt gaaagaatat gaacatacct tgcaccactt ttcttgatag
     1321 atataacagc tattagacgg gaagggagag tttgaaaatg gacttaacga atcaaagggt
     1381 cgtttttact ggaacgctgc agcaaatgac acgaacccaa gccatcggtt tggttcactc
     1441 tttaaacggc cattgtcaat caagtgttac ttcaaaaaca aatttcctgg tttgtggtca
     1501 gtattctaag tcgttgtttg atgattctct ctatacaaaa aaggaacaaa ccgcgcggga
     1561 cttgattgcg ttaggtcatg agattaccat tttatcagaa gtcgaatttt atgcgttaat
     1621 tagtcaacag ttgaaagaat ggcaacggta tttataatcg ttagtaaaat ttacaaggag
     1681 gaaaattttt atgaagcgaa aatcttatga tcgttggggc attgaaataa ctagttttaa
     1741 tcgaaatatt gcttttcacg tggagtgtga ctgtggggaa ttagcaaaaa gtgtttatca
     1801 aaaaaattac ttttactgtc ctaaatgcca tcgccgatac ggggtacatg gaacatagaa
     1861 acgaaatagt gcggtagaaa agcgaatgaa tgtagtatat ttctacaaat cattcgcttt
     1921 ctttatttta taaatagttg gtcgagagat cccgttttct tttgcaatct ctgcaactgg
     1981 aatgccttgc tttaacatgt ccaccacggt tttaaaaact aagcgttttt gcggatctgt
     2041 tgagttaata gaatactcga ttggtcgtcc cttatatcgg ccggcttttt gagccaataa
     2101 tactccttgc cgctgacgtt cacgaatttt ctcacgctcg ttttgtgcaa tataacttag
     2161 cagtgataaa aacatatcga acatagcggt atccaggagt tcatttccag tattaaaatc
     2221 taaaaactgt gcatctaata tttttaacgc tacttttttc tgtttcataa aagcaactgt
     2281 attttttata tcttcataat ctcttcctaa acgatctagt gatgtaacga tgacgcaatc
     2341 accttcacgg atataattta gtaatttttg aaactcagga cgctcagtat ctttaccact
     2401 ggctttttct tggaataatt tatcaatgtt aagtccttcc atcattgcca attgtcgatc
     2461 caaattttga tcttgtgaag atactcgaat atagccaatt tttcccataa aaaactcctt
     2521 tttaaaagtg atataattta catgtaaaat ttacttgtag ttttactgct atcatagcat
     2581 ttttaaaaat aaattttatg tagacttaaa atttacttca tggagggttt aaatggatga
     2641 aatcaaaagt aacaaaaaag catttggcat ttatttagga gaacctagta cagaacaatt
     2701 aaaccaattt tt
//
LOCUS       DACDSY010000221         2707 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDSY010000221 DACDSY010000000
VERSION     DACDSY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.224"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1141..1806)
                     /locus_tag="IWK44_002828"
     CDS             complement(1141..1806)
                     /locus_tag="IWK44_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9551280.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1932..>2707
                     /locus_tag="IWK44_002829"
     CDS             1932..>2707
                     /locus_tag="IWK44_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP9551281.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGG"
BASE COUNT          872 a          419 c          487 g          929 t
ORIGIN      
        1 gtttttcctt gtttgtttta gtttaaacta gtgtgtaatt taaaaatcta tatggaaggg
       61 tctgtggggt aatacttaaa taaaggctag gaacctgata acatcaaggt tcctagcctt
      121 tttataataa aatgaagcga aaataatttc ttttaaagag ccgagattat cagctatata
      181 agagtgaatt tgatgcaaaa aacagagccg aaacagtctt ggtgttcgta gctctgctta
      241 tatgtttcga atttaagaca aagagttggc aaaagatgtt gataaggaag ataagataac
      301 tgtgttatat aaactaggta actaactgta acaagctaat tttaagagat tcttattaga
      361 agacgtttag gaatataact aagaaaatga ttatacaggt aattgtcatt tcaataatgt
      421 tttctaattt tcgcgtcttt gttttaagaa aaaggccaga atcagttgga aaaaagttcg
      481 tacgatagtg aagaggggca aaatttccta ctaatattcc cactaacaag agataaaacg
      541 gccaggttgg tgtgattcct ctataaaaaa atgcccatgc taccgaaatc ccatagatta
      601 acaactgaac aatttttaca atgaaaaaat tgttctgcca ttactcaaag tctcttcctc
      661 cttgtatcgt ttatttgttt cattttatca ttatgatagg cgcttgaaca atccattttg
      721 tattttattg agtactcttg gataaactac cagcgattat catatttatt tatgttactg
      781 taagccatca tataaaagta aatgagcaga tcatagaaat ataaaaaaga actaaaattg
      841 atctaaatta tgtgcagctt gactacgtac caatctatat tacttactag attggtcgtt
      901 aatctattcg gatcgaatga gcaaaatatt tttgtgaatg tccatccttg attatgattc
      961 caaaaagaat cattgggagg ctgtggcgta aatattatac tataagccag aaatacctat
     1021 cattctgaac ttttttattt aggaactttc atctcaaggc tttttcaatt aatttgtacc
     1081 cctgtttttt ggataagatc atgtgcattg attttttcta tctctacgtt catctcctac
     1141 ttaatgccta tctggctcct gagggcttct ttttaaggcg tcgtcatagt cttggctgag
     1201 gatttcttgg taatctgagg gaatggcttc ccaagtgagc aggcgaacgt tcatgacgtg
     1261 ttttcctttg tgtttgatta cagcatggtt tttctctttt tctacctctc cagcgtgaaa
     1321 agacagccat gtaccatggc caaagatatc attacatacc caacgttcga tggaattgtc
     1381 cacgtatcct acagggagtg tggtatagcc gatttgtggt ttcaaaaaag gattgaatcg
     1441 atcgatcgtt tcatttttgt attgataatt gataagaaaa agatagacca aacaaaagac
     1501 gagcagaaag aaagatagtg tctttttgag tgagaagggt tgttttgatg aataagattt
     1561 cttgttaaca agaggttcat ctggcaatgt tggttctatt gattcggtag ctgattcaat
     1621 agaaggaaaa tcaatcggca gacctaaagt atgatgtaaa agtgacagca tttcaaggtc
     1681 tggcattgta cgagaatttt cccaactaga aatcgtttta tcggaaacac caattttttc
     1741 gcctaattct ttttgcgtaa gcttacaatt tctgcggtaa gttcgtattt tttctccggt
     1801 gttcatcttc ttctcctttc gattctacag tatatagatt tattctacat gacctataca
     1861 cttgatagat acgttttttt gatgaaaaac ggtatgattt tatcaaagaa tcatttaagt
     1921 aagggagagt ggtgataatc aaaaaaaagt tcacagtttt actcacaatc atagtgaccg
     1981 gtatcatgag tggttttgcc ttggttagta taaataaaca ggtactctat cggacaaatg
     2041 aatttttcca tcaaacagat acttatactc cgattcgatt gccacaacat tttattgggc
     2101 aagaagaata tgagcagcta atcatggtat ttgaaaatgt ctcatcagaa actgagcttt
     2161 cttatatgaa aaaaacagta aatgaatcca atgaagtaga tacatcttgg aacttcaaga
     2221 tacagccaaa gcttgaaatg acattgtatt atcaagggga tgtcaaatct tctcacgaga
     2281 aggtacccta ttctaatacc tcctttcgcg taacgtccat tgaagaatcg atcaaaataa
     2341 aagattttga aggtgaattt ttcttgatga cgagagaaaa agaagcctat gaacggttca
     2401 tttcactatt cactgatcga ttcaatcaag catttcacac tgattatgcc actaatattt
     2461 ttgaagactt tgaaaatagc tattcgtatt cggttatttc aaatacagac gtatataatt
     2521 atcgagtgta tttaactatt ggattgattc ttttattcct tttgttatca tcctttttct
     2581 tattgatgaa taaagaaatc agtttattgt tgaataatga cttttcatta catcaagtat
     2641 tgtggtgttt attggggaaa aagaaaatca ttattacatt cataattgca ggaatcattg
     2701 gtggttt
//
LOCUS       DACDSY010000222         2611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDSY010000222 DACDSY010000000
VERSION     DACDSY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.226"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            101..2542
                     /locus_tag="IWK44_002830"
     CDS             101..2542
                     /locus_tag="IWK44_002830"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP9551282.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          872 a          461 c          614 g          664 t
ORIGIN      
        1 ttaaggattg ttaaaattta atcttagaaa aaaataactg gaacaagagc tatttgtttt
       61 tacagcaaag aatggtatat ttttgtagga ggtggcttac ttgaaagaag tcgttatgat
      121 agatgctgca agaacgccga taggaaagta tcgcggaagt ttaagtccat ttactgcagt
      181 tgaattaggc acacttgtga ctaaaggatt attagataaa acaaaactga aaaaagataa
      241 aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt aatggtcaaa atgttgccag
      301 acagattgct ttgaacagcg gtctccctgt agatgtccca gcaatgacga tcaatgaagt
      361 ttgcggatct ggtatgaaag cagtgatctt agcacgccag ctgatccaat taggtgaagc
      421 agaacttgtg atagccggcg gaacagagag tatgtctcaa gcacctatgc tgaaacagta
      481 tcagtcagaa acaaatgaat atggtgaacc aatttccagt atggtcaacg acggattgac
      541 tgacgcattt tcaaatgcac atatgggatt aaccgcagag aatgttgcaa cacaattttc
      601 tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc
      661 tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc aagatttctg atgaagacgt
      721 gttatctgag gatgaagcag ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg
      781 tacagtattc tcagaagaag gaactgtaac agcaggaaat gcttccccgt tgaatgacgg
      841 tgcctctgtg gtgatccttg catccaaaga atacgcagaa aataataatc tgccttattt
      901 agcaaccatc aaagaagtag cggaggtcgg tattgaccca agtatcatgg gaatcgctcc
      961 aatcaaagcc attcaaaaat tgacagatag atcaggaatg aatttatcaa caatcgatct
     1021 ttttgagatc aatgaagcat ttgcagcttc ttctatcgta gttagccaag aactgcagtt
     1081 agatgaagaa aaggtcaata tatatggagg agcaatcgca ttaggccatc caattggtgc
     1141 aagcggtgca cgtatcttga ctacattagc ttacggattg cttcgtgaac agaagagata
     1201 cggtattgct tcattatgta ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa
     1261 tatggagcaa acacataagg atgtgcaaaa aaaaaagttt tatcagctta cgccatctga
     1321 acggcgcagt caattaatag aaaaaaatgt attgacacaa gaaactgcgt tgatttttca
     1381 ggaacaaaca ctttctgaag agctctccga tcacatgatc gaaaaccaag tgagtgaagt
     1441 ggaaatccca atggggatcg cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc
     1501 gatggcaaca gaggaaccat ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg
     1561 aaatatctgc gcagaaacac ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa
     1621 atcggagtat caagcagtga tcaatgcagt taatcatcga aaagaagagc tgattctttg
     1681 tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc ggtgttcaag atatctctac
     1741 tcgggaattc atggggagtt tccatgcata cttatctatt gattttttag tggatgtaaa
     1801 agatgcaatg ggcgctaaca tgatcaactc gattcttgag agtgtggcaa acaagttacg
     1861 ggagtggttc cctgaagaag agattctatt cagtatttta agtaattttg ctacagaatc
     1921 attggcttct gcctgttgtg aaatcccatt cgaacgctta gggagaaaca aagaaatcgg
     1981 tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat gcgaagcttg acccttaccg
     2041 tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa gctgtagtgg cagctacagg
     2101 aaatgataca cgtgcggtta gcgcttcgat ccacgcctac gcggcaagaa atggattata
     2161 ccaaggatta acagactggc agatcaaagg ggataaacta gttggaaaac taacggtgcc
     2221 tttggctgtt gccacagtag gcggagcttc caatatcctg ccaaaagcaa aagcatcttt
     2281 agctatgtta gatattgatt cagccaagga actagcacaa gtaattgctg cagtagggtt
     2341 agctcaaaac ctggctgctt tgcgtgcatt agttacagaa ggaatccaaa aaggccacat
     2401 gggacttcaa gctcgttcat tagcgatctc aattggggct attggtgagg aaatcgagca
     2461 agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag cagacagcta tacaaatatt
     2521 ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga agaataaagc aagatttaca
     2581 gttattttat ttactgcaga tcttgctttt t
//
LOCUS       DACDSY010000223         2601 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDSY010000223 DACDSY010000000
VERSION     DACDSY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.227"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            395..601
                     /locus_tag="IWK44_002831"
     CDS             395..601
                     /locus_tag="IWK44_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9551283.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            601..852
                     /locus_tag="IWK44_002832"
     CDS             601..852
                     /locus_tag="IWK44_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551284.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            867..1304
                     /locus_tag="IWK44_002833"
     CDS             867..1304
                     /locus_tag="IWK44_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551285.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            1297..2004
                     /locus_tag="IWK44_002834"
     CDS             1297..2004
                     /locus_tag="IWK44_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551286.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            2385..2564
                     /locus_tag="IWK44_002835"
     CDS             2385..2564
                     /locus_tag="IWK44_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551287.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
BASE COUNT          944 a          441 c          541 g          675 t
ORIGIN      
        1 ttttacatag aaacaggaaa agaccttttt aggaagacac aagtgccttc tcaattgaaa
       61 aacctttctc tcactccaac cgccaccgcc gttcgagaag aaaggaagtg aacgttgcac
      121 gttcactaag atatggtgtc ttgcaaagca agacaaaata gaaaaaactt cctctcacca
      181 aaaggttcgt tcctgttcgc tatctcttta ttgatttgcc agaccggcaa caagttgcct
      241 tttatcacca aaaagcgaca aggacaaggg taaatggcac gctgcgcgcc cttgtccttg
      301 tcgcttttcg ttgattcatt ctggccaaca aatcaataaa gaaagaacta acgaaaggaa
      361 ataaaacctt tcctaagagg aggcagaatc aatcatgcga aagaaatttg attattgggg
      421 tgttcccttc agcgaattat tcccaaacta tcatgcacca catactgtcg aatgcgattg
      481 tggagaaaga gcgaaatgta tcaaaagcta tcgtttgtat caatgtccaa catgcgggaa
      541 aaaatatacc ttgtcttatg gcgattatgt tttgattgat gagaaagtaa agaaacggtg
      601 atgaaaacac aacacaaatt attgactgta aaagaagtcg ttcaaatttt ggtggacaaa
      661 gaagtcattt ctgaaaaagt agcagaagct ttacttattc cgcggtactg gacgatagaa
      721 acccctttag tagaggatgc accgactatg ctatttgaag acctagtgaa gattgcagga
      781 tatgaactgc attatcaaga aaacatggaa acttatgaaa tcgaatattg gttaaccaaa
      841 ttagagaact aaaaaggaga aaaaatatgg ctcaaaatta ctatgacgaa tttgtcaaat
      901 tacctttgga taaaatggca caaaaaatgg aggatatgac gttcctatac catgaaacac
      961 gtgtacccaa aaaacattac aaggaaaaat tgtcagtcgc agtggaagaa atgattgaat
     1021 caggtgtcga aatgaatctc attgcgacgt actatcgaac actggaagaa ctgaaaaaac
     1081 aaaatggaaa atggtttttc caagccttac tatgcttgga agtaggtgtc aaaccaagca
     1141 ctatcaaacc ttccgaatat caagccttag aactgactta tgcaaaattc gcacagatta
     1201 agaaagctaa agcaatggct agcgactggc tagatacttt tgaaaatatc gaaaaaaatg
     1261 gtgcatttta tacgattgaa tcggaggaga aagataatga ataaggtggt ttatcagcta
     1321 acaacagaag atgtttcgca atttttaaca aacaaagaac acttgaatga agtaatcaga
     1381 cggattcaaa aagactttag ttgtatggaa gaaatgaagt actttattca aagagtggaa
     1441 gaacagttgt atatggaaag tgaaacgagt caaacgaagg ttacagtcag ccataatcgt
     1501 ttagacgtgt ataaacgtcg tcctgacaca ttaaaaaaag gagcggagct tctacgacta
     1561 gaaacagaaa aatatatcat gaatgtttac ctagaaagga gtgagctggt cgaaaacttt
     1621 gtcagctaca cgatttgcag gaaacactat tactcagaat cagaatatta ttcaattttt
     1681 gagagcatgg aattaatgga agaagaaatg gaagagctaa acagcaaaga tgcgacggta
     1741 gacgattatt accttgtatt tactagattt ttgacgtgtg ggacggaatc tatcgtgtta
     1801 atgggaatgg attatgaact cactgtaccc gaagcatttt tgagggaata tgtagaagaa
     1861 gtagataacc caggcgacga ctggtacacg actttaactg attttttaga cacctacact
     1921 tacgacgata cagaagaaat ccaaaatgaa cttgaccgta aacgaatgcc atatcaacga
     1981 tttgaaagga aaaaaggtca ataaccattt ataccgaaaa gtgaggatgt gtgaaataag
     2041 aacaaaaaac cttgaaatta agggaatggt tcttatttcg caccaatcga tccaaccgcc
     2101 cccgccgctt aggtggtgcg aaataagcgg gcaaagcccg ataacctaag agaaagaagg
     2161 agatccatat gaataaaatc attcctctat tgttcgtact gtttgttggc gagatactta
     2221 tgcgtcatgg tgcagctcta catttaaact tcgtagtatt cttaggaata ctggtatttt
     2281 gtttgatctt ttggcgcttt gtgttaaccc ttgtgagtct aatattcaag ttgattgcag
     2341 ccatattttt aatagggtta tttcgatgaa aggagagagt actaatgcca attgcaatag
     2401 gaaataaacg gttacccgtg acgttggatg aaaagagaca aaaagaattg cagcaactaa
     2461 agcagaagta cggcaaaagt gaatccagga ttatgtgtat tgcgttagat ttattgattg
     2521 cccaagaaaa agcaggattt gaggtaccag cactcaaaaa gtgacgtcac tttttatcct
     2581 aaaaactaaa agtgatagca c
//
LOCUS       DACDSY010000224         2598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDSY010000224 DACDSY010000000
VERSION     DACDSY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.228"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..2416
                     /locus_tag="IWK44_002836"
     CDS             2..2416
                     /locus_tag="IWK44_002836"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP9551288.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
BASE COUNT          882 a          472 c          571 g          673 t
ORIGIN      
        1 aatgagttac aatcataaag aaattgagaa aaaatggcaa aaatactggg caaaaacgaa
       61 tactttcaac acccatgatg atcctgaaaa gccaaaattt tacgcactgg atatgtttcc
      121 ctatccatcc ggtcaagggc tacatgtagg gcatcctgaa ggttatacgg caacggatat
      181 cctttcacgt gtgaaaagaa gtcaaggata caacgtgctg catccaatgg ggtgggatgc
      241 attcggtctg cctgctgaac aatatgcgct agatacagga aacgatccag ctgagtttac
      301 ccaaaaaaat atcgaaacat tccgacgcca aatcaattct ttaggattta gttatgactg
      361 gaatcgcgaa gtgaatacaa ctgatccgga gtattataaa tggacacaat ggatctttac
      421 gaagctttat gaaaaaggtc tagcttatga agcagaagta gcggtaaact gggtacctga
      481 attaggaaca gttatttcaa atgaagaagt gatcgatggg aaaagtgaac gtggtggcta
      541 tgatgtagtc agaaaaccaa tgcgccaatg gatgctgaaa atcactgctt atgctgatcg
      601 cttattggat gatctagaat tagttgattg gccagaaagc attaaagaaa tgcaaagaaa
      661 ttggatcggt cgttcagtag gagcaaatat tgaatttaaa gtggctggta cggataaatc
      721 ctataccgta tttacgacac gaccagatac tctattcggt gcaacttact cggttcttgc
      781 acctgaacta gatcttgtta gagaaattac gacccctgaa caaaaagctg ctgtagaagc
      841 ttatatcgaa gagacagcta aaaaatcaga tctaaagaga acggatcttg caaaagaaaa
      901 aacaggagta tttactggtg cttatgcaat caatccagtt aatggcaagg aaatcccaat
      961 ctggatcgcc gattatgtat tatcttcata cggcacagga gccatcatgg ctgttccagc
     1021 acatgatgaa cgtgactttg aattcgctca aactttcgga ctagaaattc ttccagttat
     1081 cgaaggtgga gatgttgaaa aagctgctta cacagaagat ggtacgcaca tcaactctga
     1141 atttctagac ggcatgaaca aacaagaagc aatcgataaa atgaatgaat ggctagaaga
     1201 aaatggcgta gggaaaaaag aagtcagcta tcgcttacgt gactggctat tctcccgtca
     1261 acgttactgg ggtgaaccaa tcccaatcat ccattgggag gacggaacag tcacagctgt
     1321 cccagaagaa gagctaccat tacggttgcc taagacaaaa aacatcaagc caagcggtac
     1381 tggagaatct ccattagcaa atatcgagga gtgggtcaat gtcgttgatc ctgtaacagg
     1441 taaaaaaggc cgtcgcgaaa cgaatacaat gccacaatgg gcaggaagct catggtatta
     1501 cctgcgttat atcgaccctc ataacaaaaa agaactagca gattatgaaa agttgaagcg
     1561 ttggctacca gttgacatct acattggtgg agcagaacat gcggtacttc atttactata
     1621 tgcacgattc tggcataaat tcttatacga tatcggtgtt gttcctacaa aagaaccatt
     1681 ccaaaaacta tacaatcaag gaatgatctt aggcgaaaac aacgaaaaaa tgtcaaaatc
     1741 tcgtggtaat gtagtcaatc ctgatgatgt ggtcgaaact tacggtgcag atacactgcg
     1801 catgtacgaa atgttcatgg ggccattaga tgcttcgatt gcttggagcg aaaatggctt
     1861 agaaggaagc cgtaaattct tggatcgtgt atggcgtttg atcgttgatg agaacaataa
     1921 aatgcgtgac cgtatcacta cattgaatga tggcaagctt gataaagtgt atcatcaaac
     1981 agtgaaaaaa gtgacagaag actatgaaaa tcttcatttc aatacagcaa tctcccaatt
     2041 gatggtcttt atcaatgaag cctataaagt ggacgctctg ccttatgaat atatcgaagg
     2101 atttgttcaa ctactcgcac cgattgctcc tcacatgggt gaagaactat gggcaatcct
     2161 tggaaatgat ggtggtattt cttacgcacc atggccaaca tacgatgaag cagcacttgt
     2221 tgaagacgaa gtagaagttg ttttccaagt aaacggtaaa gttcgtgcga aaagcaatgt
     2281 tccccgagac ttaggaaaag atgagctaga aaaagcagca ttagccaatg aaatagtgca
     2341 agaatatatt gaaggcaaaa cagtcagaaa agttattgcg gtaccaaaca agttagtcaa
     2401 tatcgttgct aattaattgg ctctttgtca ataaggactg atgagttgtg caaaatcaaa
     2461 tctgggccag aatgaacttc attctgtctc agattttttg ttatttcaaa ttgattaatt
     2521 gattgatcaa aaaggttttg attttcatgt tctcaaacac gttcaatgtc tttttcttct
     2581 gaatggtctt gtaagaga
//
LOCUS       DACDSY010000225         2583 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDSY010000225 DACDSY010000000
VERSION     DACDSY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.229"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1482
                     /locus_tag="IWK44_002837"
     CDS             <1..1482
                     /locus_tag="IWK44_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551289.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLL
                     DAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKGKKYTITLEGTKPATQV
                     FRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTSPQVQICQVPSTSLGNC
                     TIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEI
                     TGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDT
                     ATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDSTILTIPEGYRPPISFEQ
                     LVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            1645..2091
                     /locus_tag="IWK44_002838"
     CDS             1645..2091
                     /locus_tag="IWK44_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551290.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            2093..2230
                     /locus_tag="IWK44_002839"
     CDS             2093..2230
                     /locus_tag="IWK44_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9551291.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            2268..2561
                     /locus_tag="IWK44_002840"
     CDS             2268..2561
                     /locus_tag="IWK44_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551292.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
BASE COUNT          946 a          438 c          530 g          669 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca acagccacac cgtaccagcc taatctactt
      481 gatgcaccgt attatttgag taaggtggct ttgggtgaga atctaattaa accagaatca
      541 caacaaccag ttactaatag taactatctt attaacacct ataacattaa accaatggta
      601 aaaggtaaga agtataccat cacacttgaa ggaactaagc cagcaacaca ggtttttaga
      661 ccatttttca cacgagcaac aggagatgca tgggaggttg gtgacttaca accagtagaa
      721 gggttaactg atgtttggtc tggaacattt acagcagcag ctgacccaca ccctaccagc
      781 ccacaagttc agatttgtca ggtaccaagc acaagtttag gaaactgtac aattaaatgg
      841 ttaaaactag aggaaggcga cacacgaacc ccgaatattg agcaatataa ataccgagga
      901 atcggcatgc gagactcaaa caatccaaaa gattacgtct gggatctagc accagaatat
      961 gtcgaagaca atctggccac agatattaaa atttctgaaa ttactggtaa agcaaacaat
     1021 tataccgatg ggaaagtatc ggagattaat tcgcagttga ctgcttcaat taatgaagta
     1081 gacaccacag ctaaggatgc tcaaacaaaa gcgaatgcta atgcgactgc tatagatgaa
     1141 ttagacaata agatcgatga acgcattaat gatacagcta ctaccacatt aacagttaca
     1201 aacgggaata ccggatcagc aaagctttat cgtgaaggaa aaacagtttc tatatatttt
     1261 gtggctttaa acggaagaag cagtggtgga aatgattcaa cgatactaac aattccagaa
     1321 ggctatcggc caccaattag ttttgagcaa ctggttggct cgatagaccg ttctactttg
     1381 aacagtgctc agttatctat tggtgcagat ggagccatta aatggcgaag aaactcaagt
     1441 tatggatcgg attatacctt tgcaattact tacacgattt agaaaagcgt gaatcgatat
     1501 gaaggcagca tatagaccaa ttgaacctta cggattcgag caaatcattg tgaatgatga
     1561 agaacattta ccggaagaat gcacagaagt cgaaccaccg attccaaatt ggaaaccgaa
     1621 attcaattac tgggagggaa ataaatgaaa aacatttgga aatatggacg tactggcgga
     1681 gagtacgcag gaaaagtatt ggacgacatg cttgtatccg ttccttacac agatcagcca
     1741 ccgcttgaag gaattcgtgc tgatggcgaa ccgttaacaa tcgctgatca aatgtttgat
     1801 cctaaattga atcaatggat tattttagcg aacgcactag atcacaacga tttaaacaat
     1861 ctcaaagcaa tgtatgagtc gttagaaaat gagaacggcg atttaaaaca gatcaatgcc
     1921 aaactcatgc taagcgatgt agcaattaaa caggaaaata ctgcattgaa agaaaaagcg
     1981 gatagtttag cacaaatcaa ttcaaaaatg atgcttgctt cgttacaaaa tagcaaagac
     2041 atttcagaaa ttaaagagca actaaatcca gcttcaaagg gaggtgagta gtatgtttag
     2101 ttttagcgat gtgaaaatga tgtatgattg gggctgtttc actaacgaac aagtaatggt
     2161 tttcgttccg ttgtgcatta ctgaagaaaa agcagataaa atcattagca aagaagagag
     2221 cgcatcttaa ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag
     2281 gggaatgggg agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa
     2341 taaaaccaat tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca
     2401 atttagattt gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg
     2461 aagaactaaa gaaacacgat gaaagattag atacacatgc agaaaaattg gtagaacaca
     2521 cgcaacaaat taaaacttta tttagggaga gaagaaaatg aataataaaa cgttcgaagt
     2581 act
//
LOCUS       DACDSY010000226         2561 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDSY010000226 DACDSY010000000
VERSION     DACDSY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.230"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            277..1602
                     /locus_tag="IWK44_002841"
     CDS             277..1602
                     /locus_tag="IWK44_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9551293.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            1595..1945
                     /locus_tag="IWK44_002842"
     CDS             1595..1945
                     /locus_tag="IWK44_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551294.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            1942..2121
                     /locus_tag="IWK44_002843"
     CDS             1942..2121
                     /locus_tag="IWK44_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551295.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            2257..2547
                     /locus_tag="IWK44_002844"
     CDS             2257..2547
                     /locus_tag="IWK44_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9551296.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
BASE COUNT          905 a          390 c          506 g          760 t
ORIGIN      
        1 tcaaatgttg taaggatttt attcatttgt taatattaaa gaacggaact ccaaaaaagt
       61 ccgtactcta atttgcagtc tctcactctt ttagagtgag agtttttatt agattttcaa
      121 attaattagt aaagtgatag aataaattga tcatacaaat cataagaaga agcagggtga
      181 tagcatacct atgcttcttt ttttatgtat ttagctattt cttctttaca acatcgaaca
      241 tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc aaaagagcca
      301 gtaaatgatt actttttaat cgatatgaaa agcttttacg caagtgtcga atgtatagaa
      361 agaaatttag atccattaac aacagaactt gttgttatga gtcgatctga taatactggt
      421 tcaggattga tattagcttc ttctcctgaa gcaaaaaagc ggtatggtat tacaaatgtg
      481 agtagaccac gtgacttacc acaaccattt cctaaaacac tacacgttgt tccaccacgt
      541 atgaacctat atatcaagcg aaatatgcag gtaaataata ttttcagaag atatgtggct
      601 gatgaagatc tactgattta ctcgatcgat gaatcgattt taaaagtgac aaaatcactg
      661 aatcttttta cgactgaaga aacacgaagt cagcgaagga agaagctcgc tcaaatgatc
      721 caagaacgta ttaaagaaga gttaggtttg attgctacgg taggcgtcgg cgataatccc
      781 cttttggcta aattagcctt agacaatgaa gcaaagcata atgaaggatt tattgcggaa
      841 tggacgtacg aaaacgttcc tgagaaagta tggaatattc ccgaaatgac ggacttttgg
      901 ggaattggtt ctcgaatgaa aaaaaggctc aaccaaatgg gaattttgag cattagagac
      961 cttgctaact ggaatcctta tacgattaag aatagattag gtgtgattgg attacagctg
     1021 tacttccatg ctaacggaat cgatcgaaca gatatagcaa tcccgccaga accgacaaag
     1081 gaaaaatctt atggtaattc acaagtgtta ccacgagatt atactagaag aaatgaaatc
     1141 gagctggtgg taaaagaaat ggcggaacaa gtagccattc gtatcaggca gcataattgt
     1201 aaaacaggat gtgttcattt aaacattggt acgtcgatct tagaaacgag accaggattt
     1261 tcacaccaga tgaaaatacc tatcacagat aatacaaagg aattgcagaa ttattgtctt
     1321 ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat aacctatagc
     1381 aagcttgtgt acaccgattc gcttcagctg gatttattta gtgatccaca aaagcaaatt
     1441 aacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg ttttacgtcg
     1501 attgtacatg cttcttcaat gttagaaagc gctagaagca ttactagaag cacacttgtt
     1561 ggtggtcatg cgggcggaaa tgggggaatt aaaaatgatt gattacaatg atcgtggacg
     1621 tttaaagtgg gcagggtttt atttatctga tcatactgaa gaaattgatg ccgacaatga
     1681 acaacgaacc agtcaaaatt tagcaaaaga acaaatgacg actgaagaga ttgcggaagt
     1741 attgaacgct gcagtcataa aaaatagatc agtatctatt caaaaagaag aacgaaacgc
     1801 tgaaggatac tatccagaag atattgttgg caagatctta ggtagtgatg agctggggat
     1861 ctacgttgga aaagacaaaa ttcactacga cgaaatacgt catatcgaat tttaccagga
     1921 taataaatgg agtcgattag aatgaacatt atccagcaat atgagctaaa gtatataacc
     1981 tttgatcagc tatcagaaga gatttggggt tatggccaac gtttgataaa tgaggttggt
     2041 gttgagcgct tctctttcta tgtagaagct gcagcaggtt atcataattt taggttttat
     2101 attttcccgt tatatatata aggtatttta cggattctat agagttatct ctatgaatcc
     2161 ttttttgttt ttacaaatag gagtagatac tagaataatg cgtgtgttaa actattattg
     2221 caaacgcaat aataaaagaa agtaggctta cgcaccatgt ctatcaatac atttgtttac
     2281 tcgcacccga tcaatgttta cattatcaag aatttaggaa tcactgtaga acaattttgt
     2341 gagttatatg cctacccaca aggaacggtt gcaagctgga taacccgaca acgtagaata
     2401 aaatcattac ctgcttcatt cgtttatgat ttaagcctgg cctctagttt aaacatgtct
     2461 gatgtttatg aaaagctcct gtctttagaa aaagaatatg attccttcaa gattaaacat
     2521 gataaaaaaa taaaaaaaca tatataatat caatcacaat t
//
LOCUS       DACDSY010000227         2453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDSY010000227 DACDSY010000000
VERSION     DACDSY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.231"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            132..344
                     /locus_tag="IWK44_002845"
     CDS             132..344
                     /locus_tag="IWK44_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551297.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="IWK44_002846"
     CDS             506..1024
                     /locus_tag="IWK44_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAP9551298.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="IWK44_002847"
     CDS             1031..1543
                     /locus_tag="IWK44_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551299.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1814..2437
                     /locus_tag="IWK44_002848"
     CDS             1814..2437
                     /locus_tag="IWK44_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9551300.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          943 a          310 c          408 g          792 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaaaa
     1561 taaactaatg tccttatttt tgtgttcaca tttctgttac ctaagaaata cttaaaggag
     1621 gtgatgtagc cagcaacgac tacaacttga acccgctatc tacagtttat ttgttgtttc
     1681 ttttgggtat actctaatgg tacaaatcca tatttgctta aagcgttgat ttttcagcgg
     1741 tttttcttta ttgcaaagtg taccctaaga cctgtatact aatagtaaat agattggata
     1801 ggaatgatgg caaatgtatt tagcttattt aagagtttct tccgacgatc aaagtttagc
     1861 tcggcaacat aagctaattg aaaattggtc agtcaaaaat aatattgctg aagacgattt
     1921 aaaaattttc gaggaaaaag tctctggaaa aaatattgaa aacagaccag agctgcaaga
     1981 gcttatttct ttcatacgtg aacgagatac gattattatt cctagtcttg atcgattagc
     2041 aagaaatagt aaagatacaa aagacttact tcaattgttt cgtagtaaag gtgcttctat
     2101 tgaaatatta gacctaccgt cgtttaatgg ggtaactgac cctgcattaa gagacttgct
     2161 aacaaattta gtcatagaag tgtttagcta tgttgctgaa aacgaacgga aaaaaatcaa
     2221 agagcgtcaa aaacaaggca ttgaaattgc aaagaaaaat ggtcgttata ctggtaaaaa
     2281 gattcaatac catgccaatg caacaggaaa aaacaaattg atttacgatg aaattgttga
     2341 tggtctgaag aaaaaagaaa ctatttctaa aatagcaaag aacgcaggag ttacaagaaa
     2401 aaccgtttat cgagtaaaaa gagatttagg aatgtaatat gttccagtta gaa
//
LOCUS       DACDSY010000228         2449 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDSY010000228 DACDSY010000000
VERSION     DACDSY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.232"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1448
                     /locus_tag="IWK44_002849"
     CDS             <1..1448
                     /locus_tag="IWK44_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9551301.1"
                     /translation="LQNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPA
                     EYMEDILSKDRSPTYLVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRS
                     LSFISEDMPDATLILDVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQ
                     IAIGNHTLQVYENPNYSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEII
                     TSLNHSSTGDYKSINIENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAA
                     LQYQIEPHFLFNNLEFIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELIN
                     IDLSLQLHQRRYRHNFDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVY
                     ATETTNHYVLKIVENGKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDT
                     RFVIESQLHVGTTITIKIQKGGNMHVENCNC"
     gene            1426..2154
                     /locus_tag="IWK44_002850"
     CDS             1426..2154
                     /locus_tag="IWK44_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9551302.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
BASE COUNT          884 a          438 c          383 g          744 t
ORIGIN      
        1 acctgcaaaa cattcagcaa atcacccact atatggaaac atataatcgt attctctccg
       61 gtatcgtgac agagatctat caaaattatg atttccaaga aaaccttaat gactactacc
      121 tctcatcacc tgctgaatac atggaagata ttttgtcaaa ggatcgctcc cctacctatt
      181 tagtagataa cttaaaaaaa cttgtgcaag atgatgaagt taaaaatata tctatctcat
      241 tagattacga aaaatactta agtgcctacg cacctacaaa aaacgaaaaa atttctttta
      301 ttcgttcatt aagtttcatt agtgaggaca tgcccgatgc tacattgatt ctagacgttt
      361 cgatcaacgc gctaaaaaag agtctctttc caacacatta caattattct ttgtctaatg
      421 gtattgattc cgtcaattta tctgaagaca tctcgccttc taaatggaaa aaaacgcaac
      481 agatagctat cggtaaccac acattacaag tatatgaaaa tccaaattat tcgtcacaaa
      541 ccttattagg tctggcaatc atgattttct tattaatgag tttgtttatt cttgctattc
      601 atcaattatt aaatagaagt tttcgtaact atatccagca gtatcaggaa atcattactt
      661 ccttaaatca ttcatctaca ggagactaca agtcaatcaa tatcgaaaac aaagtcggtg
      721 atttgaaaga aatcgctatg catatcaatc agttttattt agataatgct acatatcaaa
      781 aaacacttta tgaaaataat tggttgaagc aagaagcaaa gtatgcagca cttcaatatc
      841 aaatcgagcc tcattttcta tttaataatt tggaatttat tcgaatgact gccatattga
      901 atgatgacga agaaatcagc gacagtatat atttattgag caagttgtat cgcaataata
      961 catatcagcc cgacataatt gatttgaaag aggaattaat taatatcgac ctctccttgc
     1021 aactacatca acgtcgttat cgacataact ttgactatca gctgattgat catattgatc
     1081 aattaatgtt accaaaactt tctttacagc catttgttga aaattatatt aaacacggct
     1141 tcgattcatc aaaaaaggaa aatcgtttga ctgtctatgc aactgaaaca accaaccatt
     1201 atgtgttaaa aatcgttgaa aatgggaaag gaatggacca agacaagctg gagcatattc
     1261 aacaaaaact taatgatgat attccttata gtgagaatat cggcatctca aattcttttc
     1321 gacgtataaa gcatctctat cctggagata cacgttttgt tatagaaagc caactacacg
     1381 tcggaacaac gattactatc aaaatacaaa aaggggggaa tatgcatgtt gagaattgca
     1441 attgttgacg atgattgtac aattgtagaa ggcataaggc attatctttt gaagaaagtc
     1501 cccgacatca ttatcgatca ctgctacaca gacggagacg aagcatttaa aaacctgcaa
     1561 aaccagcgct ttgatttact tatcacagat attacgatgg ggaaaatttc tggactcgaa
     1621 ttaatcgaat ctttgcaaca aacatccaat cgctacttta ctagtattgt cattagtgcc
     1681 caccaaaatt ttcagtatgc aaaaaagggg attgatttat ctattaaaag ttacataacg
     1741 aaacccattg attttcctta tttagttaag ttagtacaag aagaaaaaat gcgtaaatca
     1801 gcagctaatc aaaactctct aactgatgat tcttttaatg agtcaattca acaagtgatt
     1861 gattacgttc aaaacaatta tagtgaaaag cttacgctca aagatgtagc tacacaattc
     1921 cattaccatc ccgcttattt aggacgggcc ttcaaaaaag aaacaggtta ttcctttcat
     1981 gattacttgc tagactttcg aatcaaaaga gcaagtgctc tactgaaaga cacattctta
     2041 tctattgatg aaattagtca aaaagtagga atagatcacg tgagctattt ttctgttctc
     2101 ttcaaaaaaa ctactggaca cagccccagt aattaccgta aaaagcttct ataatttctc
     2161 agacgtgttc atcttctagt tatacttata caacaaggag ccattgaaaa gtggtgattt
     2221 ttggctatta accatttaat gatggtagag aaaaatacat tagaatattt aatcagatcc
     2281 agttgagcat aacgcatagg attttcctta tgtcttcaac ccccgctgag gttgaggaat
     2341 atggaaaaaa ctactatgca agtcgagtgc aaaaatggcg ctgaactatt taactgaaat
     2401 gaaaaaggct attttagaag atagaataaa tggatgtagt tatttaaat
//
LOCUS       DACDSY010000229         2444 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDSY010000229 DACDSY010000000
VERSION     DACDSY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.233"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1372)
                     /locus_tag="IWK44_002851"
     CDS             complement(<1..1372)
                     /locus_tag="IWK44_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9551303.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYL"
     gene            complement(1391..1795)
                     /locus_tag="IWK44_002852"
     CDS             complement(1391..1795)
                     /locus_tag="IWK44_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP9551304.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(2027..2284)
                     /locus_tag="IWK44_002853"
     CDS             complement(2027..2284)
                     /locus_tag="IWK44_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP9551305.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
BASE COUNT          745 a          465 c          335 g          899 t
ORIGIN      
        1 gaagataata atagtcaaat tctatttgaa aatctctctc tatttcctta acatactgtc
       61 taaatcgaat ctcttgatag gtgggatatg ttaaagctga attattttgg atatcaacat
      121 tcttttgtct aaaatatcta tcatatttct caaaaagctg ctgcttatca gtgtctttct
      181 tccacgtttg ataaagatac tctataagtt cgttgatatt aaaaacttcg tctacttttt
      241 ccaaatcatc catttgaata tattttaatt cattctgtct tttcataacc ttcatgccgt
      301 tgaaattcac ccattgtggt aaatgataca tctgaatata aagttcttcc aaatcatcta
      361 agaaataaaa ggcattggca caggtcaatt gaatgatatt tttcaaattg ccaacatttt
      421 cactaaagcg atagtttaat aaaaaatcta ataattgttg agatatcagt attttctttg
      481 ctagtctttc ttcttctgat ttaaaaaata tcttgattaa ctgttttctt tcataaagag
      541 aacgactgtc taatgttgga agtgttagct ttacaggtat tcttctcaac aaagttgata
      601 atagtttatc ctcaggtggt tctgatgttg caaatataat ccagcaactt gatgtgtacc
      661 agttttcatt atcacccatc cgatgaaatt tcctcgtatc cataaactga aaaagtttct
      721 cctgacattc attgctcaat gcatgaattt catctaaaaa taagactcca ttattagcta
      781 attctgctaa accctttctt ccatcttcgg ctcctgtata tgcacctttt acatgcccaa
      841 aaaaattgga caagaaaagt tctggattat tggcatattc actacaatta actgaaatga
      901 aagatgcctt ctcttctata taattattgg ccaacaaatc cttatacaca taatttgcta
      961 aatagctttt tccagttcca gtctcgccat gaagtaaaat gggtaagcca tttggcggat
     1021 agtagattgc tgctttaatt tgagcaatta catttcgcag acttccttga cttccgataa
     1081 ccctttctat tccaacccta aaagagctat cataccattt ttccgcttcc tctgaagagc
     1141 taaaaatttg ttgatttgga actttctccg gtaaagaaaa gaagattact ggacgagtat
     1201 tgatttttcc aaattctcca tcttttacga attcattcaa atattgagag acaatattac
     1261 gactaacatt taatttttca gatattttct gtgctgtatt gttcccccat ttatttttaa
     1321 tctcagagtt ctctatgaca taattgacaa tgcgttcttt taattcttcc atcacagcac
     1381 ctcctcgaca ctattcgaat tttgccgaca gtatttttcc ataaatatga cttgctaaaa
     1441 aggctttgtt tagatcattc aattggaacg ttgcttgttt tgcttgatcc gctaagacgt
     1501 tgaaaagata ctctggcgaa atcgcatacc ccaaaatatc aacgattgtt gcaatcagat
     1561 cttcttttga ttcttcatct gaaagtaatt cacgatacaa catcgtttgt ttgcttactg
     1621 tgtcatattc ctctaaggat tcatctgcta aaagaaccac ttgttccaac aaacggtcgg
     1681 ataaatattt atagaaaatc agccccaata aataattttt atattctgac gcatccattt
     1741 tactacggag attatctgcc gcactaaata acttctgatt taattctgca cccaaaaaga
     1801 tacactccta tctcttattt aaagttgcta tataccctct tattttagca tactcagttt
     1861 tagaaaaagc aaaaaaataa agaaacttat tcttgataac aagctacgtg acgtatatcc
     1921 ctaacacaaa aatagccata tcctttggtt tggatacggc tatttgatat tactcattca
     1981 attatcaaat cacagtaggt gtgaatctca tcgggatcgg caatatctac ttctgattag
     2041 gatcaaaggt gattttctca ataaaggtgt gatacaaagc tttaacttgc ttcttctcac
     2101 tctggataag aaagcaatcc aaactagtca atatctgttg gacatctttg acttgaatcg
     2161 gttccccttg ttgttgaaag atccctaaga tttcattctc tcgaatttgt aactgtcttc
     2221 gtttttcagt caattcattt aaacgatctt ttaacatcgg aaacaattcg ggactatctt
     2281 ccaaagcagc ttcctgtttt tgctctaata gttagatttt gcgtaccatt ttcttctttt
     2341 attttccttc gattgctgat tttaatatag tctaaaggaa atttatattt attgtattct
     2401 ttataacttc aagagaactt cgtttgagta ttactgtatt tttt
//
LOCUS       DACDSY010000230         2408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDSY010000230 DACDSY010000000
VERSION     DACDSY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.234"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(243..464)
                     /locus_tag="IWK44_002854"
     CDS             complement(243..464)
                     /locus_tag="IWK44_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551306.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="IWK44_002855"
     CDS             complement(562..807)
                     /locus_tag="IWK44_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551307.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1047..1661
                     /locus_tag="IWK44_002856"
                     /pseudo
     CDS             1047..1661
                     /locus_tag="IWK44_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2031..>2408)
                     /locus_tag="IWK44_002857"
     CDS             complement(2031..>2408)
                     /locus_tag="IWK44_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP9551308.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          816 a          419 c          332 g          841 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
      961 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     1021 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     1081 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     1141 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     1201 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     1261 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     1321 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     1381 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     1441 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     1501 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     1561 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     1621 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     1681 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     1741 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     1801 cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa
     1861 gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc ccccgcattt
     1921 acgattgcac aagagaaaca taaagtcgac ctattagcat ttcttttcaa ttcaaacatt
     1981 aaaaaagttt atacctctgc tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt
     2041 aaatctgatt catccaaatt ggaaaatgga tcagcctgta ttggagcttt gtatttctcc
     2101 ataataacag atatcttttt ttcatttttt aaaaaatacc ttttaaattt cttatagtcc
     2161 tctgaaatca aaatatagcg tgatggatac tcatcaaaag aagaatcgta ttcttccttt
     2221 aaaattcccg atttacttaa tttttctagt acatcaaata ctctatcact caagtaactc
     2281 cttaaattgt tatcactttc ccattgaact acttttctat aagttgaatt atcagttgaa
     2341 aagatagaat tttcatccat aaacgcatat attagcagca atctttcatc attgtcaaat
     2401 tctattgg
//
LOCUS       DACDSY010000231         2400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDSY010000231 DACDSY010000000
VERSION     DACDSY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.235"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IWK44_002858"
     CDS             207..2069
                     /locus_tag="IWK44_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9551309.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="IWK44_002859"
     CDS             2069..>2400
                     /locus_tag="IWK44_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9551310.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DACDSY010000232         2383 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDSY010000232 DACDSY010000000
VERSION     DACDSY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.236"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            83..2236
                     /locus_tag="IWK44_002860"
     CDS             83..2236
                     /locus_tag="IWK44_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP9551311.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          812 a          423 c          530 g          618 t
ORIGIN      
        1 ttgctccgcc cattttttct tttttattaa gaagtttttg ggtacaatga agcatgaatg
       61 taaataggcg gaaggatagg aaatgatgaa agattttatg aaaaaaatat taaatacttc
      121 ttggttagaa aaaataagaa aaggtgcaca agaacctaag aacaaaacgt ttcgccggtc
      181 ccatgttcct tttcgcttaa attttctttt tttcattata tttacgttat ttgttgcgtt
      241 gattgctaga ttgggttatt tgcagatcgt gaacggcgaa gagatggagg cgcgagtgaa
      301 gtctacttct acgattacgg tccaagaaag tactcctcgc ggaatgatct acgactccac
      361 aggaaaagct ttagttacga ataaagcgaa tcaggccatc acatatacga gaggaacaca
      421 agttacggct aaagatttgc ttgaaatcgc aacgaagctg aactcgctga tcgatgttcc
      481 ggtagatgaa aatctgacag atagagataa aaaagatttt tggctggctg atcccaaaca
      541 tttgaaagaa gccacaaaaa gactttcggc aaaagaaaag caattaggga acagcgaaca
      601 atatagtgcg atggtcaata aagtcacagc tgatgagatt aatttaaacg aggagcaatt
      661 aaaaattgct accatcttta aacggatgaa tagtgcatat gaattaaaca ctgtctatat
      721 caaaaactct ggtgttactg atcaagagtt agctgttgtg gcagaacgcg cttctgaact
      781 tccaggagtt tcgactggca cagactggac acgagaatat aatgcagcaa gttcactgaa
      841 aagtattctt ggcagtgtga cgactgaaaa gcaaggactt ccagcagatg aagcagagaa
      901 atacctagca aaaggttatt ctcggaatga ccgtgtagga caaagttatt tggaaaaaca
      961 atatgaagat gtcctgcaag gaaccaaaac ccaatatgaa gtttcccttg ataatgaagg
     1021 aaatgtttcg aatcaaaaag aaatcttctc tggggaaaaa ggttctaatt taatgttatc
     1081 tatgaatgca gaattccaat caaaagtaga agaaattttg aaaaggaatt atcaaacatt
     1141 gatcaacaac ggcaaagcac agtattcgcc tggtgcttat gcagtggcta tgaatcctca
     1201 gacaggagaa gtgctagcaa tgactggttt cagtcatgaa caaggttcca aagaaatcac
     1261 cgagaatgct ttaggcacga ttactagcgc ctttgatcca gggtctgtag tcaaggctgg
     1321 tactttgaca gcaggctggg caagtaacgc gatcagcgga aatcaagttt tgattgatga
     1381 gccgattcgt ctgcaaggtg cttcagaaaa aagctctgtc tttaatcgaa gcggacaagt
     1441 tgctcttgat gcagtcaaag catcggaatt gtcttctaat acgtatatga tcaaagtggc
     1501 gttaaagatg ttaggactag actatacacc tggaatggga ctacctagtt tagatgaaga
     1561 agctaaagct tatcagcaat tgagagactc tttcaaagag tttggtcttg gtacaacgac
     1621 tgggatcgat ttgccgaatg aatctcctgg gatctcacgt tctgttgatt atatgaagaa
     1681 gttcaatgcg gataatggca aagaatggta tgctccaggt aactttaccg atttggcatt
     1741 tggacagttt gacacatata caccaatcca attagctcaa tatgcttcaa cagtcgctaa
     1801 tggcgggaaa cgagtacagc cgcacctagt caaagcgatc tatggcaatg acgaaaatgg
     1861 aaacttgggt gaagtgaaga aagaaattgg cacaacagta gaaaatacgg tcaatatttc
     1921 tgcagaaaat atggcgatcc tccgtgaagg attccaccaa gttgtccatg gtaccgatcc
     1981 ttacacaaca gccaaaccat tagccagtgc gaaaatggac ctttccgcaa aaacagggac
     2041 tgcagaaacg gttgcggaag gtcatccaga tattgcgaca gtcaatagta atatcgtggc
     2101 atacggacca acagacaatc cggaaattgc catcagtgtt gtcttgccga acttattaga
     2161 cgaacaagac cacataaact tgacgattgc taaagaaata atggacgcgt attatgatat
     2221 gttcatggca aaataaaaaa gaggtcgtga aaaagccgct tagtttcgag tatgaagaaa
     2281 aattttgtga aaatagcttc tcacattttt gcttaaattt gacttcatac cgagaaactg
     2341 gcttttgaac accgtttact cggcaaaaaa ggttgtgaca aaa
//
LOCUS       DACDSY010000233         2345 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDSY010000233 DACDSY010000000
VERSION     DACDSY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.237"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..270)
                     /locus_tag="IWK44_002861"
     CDS             complement(<1..270)
                     /locus_tag="IWK44_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551312.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..947)
                     /locus_tag="IWK44_002862"
     CDS             complement(276..947)
                     /locus_tag="IWK44_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9551313.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(940..1281)
                     /locus_tag="IWK44_002863"
     CDS             complement(940..1281)
                     /locus_tag="IWK44_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551314.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(1455..2180)
                     /locus_tag="IWK44_002864"
     CDS             complement(1455..2180)
                     /locus_tag="IWK44_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9551315.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
BASE COUNT          624 a          481 c          351 g          889 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcgataat ctaaaacagg aaataactct
      661 gtttccgtat gtcctttaac cgttactgag acagatacat aagtccctgt ttcatccata
      721 agaaaaggtt tatattcctc aacaagaaag tcttgatgag ttccagaaac aaccctgtag
      781 tgtttatact cattaatagt taccgttgcc tgtggatcat tctttttcat aatctcccac
      841 gcgtgagccc aagataaata atcaaaattt ccttttttct tgagaatttt atttaactta
      901 cgactaaaaa gtttttcaaa gttcgttgtc cctttgattt cactcatcaa attctgcctc
      961 catttcagca atgtatttct tacctgatcc gtaataagag atatcaatca agttatctct
     1021 gttgtactct tctagcgcat caatcaagcc atcttcgatg acatagatgt attcaggttt
     1081 atttgaatgt tttgatagat ggataaggta aacatgatcc caaatactca caaaatttcc
     1141 caagtcatct tgatcacatg atagttcttc gttcgtcaaa agatttcgtc tgatttttcg
     1201 attatttgtt tccttgatat tcgacttgcc ccattcagaa tcagtcaaat attgatctaa
     1261 agtggaaagt tctttattca tatgctaaaa ttctccttag atatgttttg tgactctatg
     1321 cttgccggct gagtcacttt tttatttgtt gccatgcttt ttgcttttcg atatgttgtt
     1381 ggcttaggat gtttggttta ttgtgtctcc accagcgatt agcaattacc gtccctattc
     1441 ttagcgcttc agctctattc attttcatca ccgaaaagtc tttgttgtct gttcagttga
     1501 tcgatttcca tgcggatcgc agtttctggt aaccacattt caataaatga aacagcatca
     1561 tcgaatctct tacgaggtaa ctcgccatat cttgggattg aaaaggtacg tttaaattca
     1621 gaccaaaatt ttgagaatac ttttttgctg atttcttcat aagctcggct ttcttttccc
     1681 cctagaactt ccataacttt catatttcct ttttgcttaa tttcaaactc ttgttgtccg
     1741 ctaattcgca tagtatcttt aagcatggaa acatcttttt taacatcttt catttcttct
     1801 aattgataga tcatcatgtc ttcaattgtt tgaggaacag tattcttccg aataacatct
     1861 tccatttcgt tgaaagcttc aataaatttt agtttgaaat ttattgcttt acttccagtg
     1921 aatcccatag ctagcaagga aaatccgtct ctattcatga aataaactct ccgacttctt
     1981 ccgtatgagt ctggctcgtt cccttccaca aacatctgtc caaaattgga cccatcttca
     2041 acattggccg aattttcgac cgatcttttt attgattcaa ttgcttctag tacatgctta
     2101 tgtttctttt tgaaactttc tgccacttgt aagctcgtag ttacagcttc tttatttttc
     2161 aaaattacta attcttgcat tatttcttct ctcctttttg gtataatttt cgttagaaag
     2221 tgaggtgatt ctatgaggaa aaagctcaat tacattctta ctgcgacttt aaccatatat
     2281 ataattctgt ttattacctt atctttcctg tcttcaatta aaaaaattcc ttttattaca
     2341 ttgaa
//
LOCUS       DACDSY010000234         2304 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDSY010000234 DACDSY010000000
VERSION     DACDSY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.238"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            94..1494
                     /locus_tag="IWK44_002865"
     CDS             94..1494
                     /locus_tag="IWK44_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9551316.1"
                     /translation="MWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIY
                     YRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIA
                     CMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTD
                     LQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTW
                     VCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGA
                     TVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERP
                     HLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVND
                     SYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKD
                     SRYVTKKEINKKNENK"
     gene            1481..>2304
                     /locus_tag="IWK44_002866"
     CDS             1481..>2304
                     /locus_tag="IWK44_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551317.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAM"
BASE COUNT          764 a          339 c          392 g          809 t
ORIGIN      
        1 ggactgaact atttttatcc ttttcttaca ataattcctg cagtcgtttt ttatggaatt
       61 tcaaataact taatattaag ttatgttcta tatatttggt tattaaatat ttgtacgatt
      121 ctgattagtt tttattatgg agaaaagttt tttaaaaaga tagatgctgc ttttttattt
      181 agttgtttat atacttttgc aacttatcga acagttgata tctattatcg atccgcaatt
      241 gcagaagcaa tcgctcttac gattatccca gctgttttat attatgctta tggattgata
      301 ttcaatagag aaaaaaaagt catcgcttta tctgtcagta taagtctact tgtgtatact
      361 catgtcttaa ctacactatt gtgtagtgca ttgattgctt gtatgattat agttcgtttg
      421 tttttaagca agcaaataaa aaacgatata aaatttgtgg tcgtgaattt agtaaaggct
      481 gtttttttga cttgtgcatt aactagctat ttttggtatc ctatgttgca gcagatgaat
      541 tatcaagaaa tacacagtcc cttcaaaaca gatttgcagc tagaagcact ttctatttca
      601 gatagtatca ttcaagcgtt aaataacgat attacaacat acactatggg gttattagga
      661 ttggttgcgt tgatttttcc tttattcata ctgaaagaaa tggagaaaaa agaaaaaatc
      721 atctatgttg gcgtagtttc tacatgggta tgcgtaacta atttatttcc atggtttttg
      781 tttcaaaaaa caccgataag cttactccag tttccttggc gtatcttagg tatccaaatc
      841 tttttttctt caatgatcat aacgcttgtt tttatgaaaa agactatgaa taaaaaatat
      901 tcatggttaa tcattggcgc tactgtggtg tttctactcg tcacgtctat agcagcaaaa
      961 gaaaattacg caaaagttat ccaatcaaaa cacggtcata ttgttatcaa tgaagaaaca
     1021 ctcccacgtt atacaacatc agatatggga ggattatacg attacgcccc aacagaagcg
     1081 ataaaagaaa ggcctcactt agaaaaacat gaagtaaaag taggagaatc gtgggaaaaa
     1141 gggaagtata aagctgcaga taataacatt acttacacag tggcaagcaa caaaaagcaa
     1201 aaagtgacag taccagtata tgcttattta ggtacacagg taaaagtaaa cggtaaagtc
     1261 gtaaatgact cttataaaaa acatggctta gtgaatgtgg atgtaaatgc tggagaaaat
     1321 aagatagaaa ttacaacaaa atatactcca actgctctca tggctttatg gatcagtatt
     1381 ctgacttatt gtttagtggt gtattcaagt tttaaacgat ttgcacctaa taagttgaaa
     1441 gattctcgat atgtgactaa aaaggagata aacaagaaga atgaaaataa ataaaaaagt
     1501 agtaatattg agtggattaa ttatagttag tagtatatat tctttcatct ttttcaaaaa
     1561 tacacagagt atttatacca ctggagatct ttcttttcac ttatctcgaa tcaaggggtt
     1621 atcgactatt tttaccagtc cgattaatta tgagacattc aattatactg gatatggtgt
     1681 aaattatttt tatccctttc tgactttttt tccagctgta atgttatatt ggatgacaaa
     1741 aaatttgatt gtaagctata ttatttacgt ttggttattg aacttgtgta caacactcgt
     1801 agcttatcat tatggcgagc ggtttttaaa acaaaaaaaa gcggcatttt tatttagttg
     1861 tctctatatt ttctctgctt atcggacagt agacatctac tatcgttccg caattgccga
     1921 agctatcgca ataacacttg ttatccctgt attattttat gcttatcaaa tcatctctgg
     1981 aaaagaagaa aagtatccgt ctgtaaagct agcattgagt atgagtttgc tagtgtacag
     2041 ccatgtacta tcaacattaa tgagtacagc attaattatc atttttattt ttattcgttt
     2101 ggtttctaaa ggtttcaaga atgcagattt tattgctata tttaaaaaat tatttagtgc
     2161 ggcaggaatg acattggttc tgacgagtgc tttttggtat ccgatgtttg aacaaatgct
     2221 ctaccaaaaa atcaacaaac ctagcgtgac aaatttatat gctcatgcat ctaatgtgtt
     2281 cgatagtctg actgaagcta tgaa
//
LOCUS       DACDSY010000235         2290 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDSY010000235 DACDSY010000000
VERSION     DACDSY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.239"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            64..2229
                     /gene="acm"
                     /locus_tag="IWK44_002867"
     CDS             64..2229
                     /gene="acm"
                     /locus_tag="IWK44_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP9551318.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
BASE COUNT          904 a          352 c          440 g          594 t
ORIGIN      
        1 aaggattttt gagagatgat atagtaggtt ttatttttag ttaaaatagg ggagatagaa
       61 aaaatgaaaa agtgtagaaa tattatttta tcaatgttat ttattattac taatataaca
      121 tcactgattc ctgttcatgt ctatgcggat gcaggcagag atatcagcag taatgtcact
      181 tcgttgaccg tggacccaac gaatattaca gatggaggaa acataaaagt aaaattctcg
      241 tttgacgaaa aaaaacaaaa tatccaacca ggtgattatc tttggatcaa ttggccaagt
      301 gaaggaaata ttcgcggtga aggattccaa aaagaaatcc cattaatgat tgaaaataaa
      361 aatgttggaa ctttaacggt cagaaaagat tctgcgcagg ttgtgtttaa tgaaaatatt
      421 aaaaacctag actcagtaga aggttggggg caatttgaga tacaggccag aaacgtaacc
      481 gataccggtg aagaaaatac gggaccattc accgtgacga gcggtgataa aacagctact
      541 gtaaatgtga caaagccagc ttctggttct tcttctagcg ttttttatta taaaacagga
      601 gacatgctac ctgaagatac taagcacatt cgatggtttt tgaatattaa caataacggc
      661 acttatgttg aacaaccagt taaaatcagt gatgaaatcc aaagtggaca aagattagat
      721 ccaagtacgt ttgaaattaa tcaaatacac ttaggtgaac agaaggttta tagaggagaa
      781 gagggaatcc aacaattttt acaagatttc cccagcgcta catttaattt ttcagtaact
      841 gataattaca ttgaaataac tataccaaaa aactttgtca atcttaggaa aataatggtg
      901 agttataaga cgatcataga aaacccagaa caaataaatt ttgagaatca ttcggaagct
      961 tggtttaaag aatttaataa acctgctgtt gatggagaaa gtttcaatca tacagttaaa
     1021 aatatttcag cttctggtgg agtcaacggt acagttagag gcgaattgaa gattttcaaa
     1081 tatatcaatg acacagagat tggtatccca aatgtgactt tcgaattaag acgagcagat
     1141 gaacagccta ttcaaggaca gtctagtatc ctgttgactt cgaatgaaca gggagaagct
     1201 agtatcaaag gcctgcaagt aggagactat gttgtaaaag aaaaagaagc acccaattgg
     1261 attgattttg atccactaag ttcaaatgaa ctcaaattct ccataaacga aaatgataca
     1321 gagggagtca gtttacctat ttataacaag aaaaaggtta cgaacatcac agcaactaaa
     1381 atttggaacg gtggaaccac tccaagaccg agtatttatt ttaaattatt cagagcaagt
     1441 acaaacaatt gggaaccagt tcctgatgca gaaacaaaac gattagacaa tggaataact
     1501 tcagtgactt gggaagatat ccaacagtat gatgatagtg gaaacgaata tacgtttaaa
     1561 gtgcaagaag tagatcaaaa cggaaatgat tatgtccctt caggttatca aaaaattgaa
     1621 aacgggctag tcgttacaaa tgagaataaa gaagtcattt cagtctcagg acaaaaaaca
     1681 tgggaagaca atgagaacca agacggcaaa cgaccgacga cgatcacagt aaatctatta
     1741 gccgacggtc aaccgatcca acacaaagaa gtaagtgaaa aagatgattg gcgctatcgt
     1801 ttcacaaatc tgccaaaata cagagacggg caagaaatca tctatactgt aacggaagac
     1861 aacgtgcccg aatacagtac gacgattgac gggtataaca tcaaaaacag ttatacaccg
     1921 gaaaaaacaa atatatctat accaggtagc catataacac cgtggaaaaa ttttccagag
     1981 aagacagaga gagagaaaat tagaaatcta ttctctcaaa aattgcaaat atttgctcat
     2041 acaggagaat ccagcgaaca aagaagcaat tatattagta agagagctgt acctaaacag
     2101 ataacaaata aatcaaaaag cattcttcct aaaacaagta gtaaaaaatc tagttttgct
     2161 gtaatcattg gtttattgat agtaactatc tgtgctggga tattcttaca aaaatataaa
     2221 aaagtttagt tagaatataa tattttttgt taaatatgac tgaaatcaaa agaataaaaa
     2281 tccgatttat
//
LOCUS       DACDSY010000236         2232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDSY010000236 DACDSY010000000
VERSION     DACDSY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.241"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IWK44_002868"
     CDS             <1..378
                     /locus_tag="IWK44_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9551319.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IWK44_002869"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IWK44_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9551320.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..2026
                     /locus_tag="IWK44_002870"
     CDS             1859..2026
                     /locus_tag="IWK44_002870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551321.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
     gene            2080..>2232
                     /locus_tag="IWK44_002871"
     CDS             2080..>2232
                     /locus_tag="IWK44_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9551322.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVREILYDRGINVSH
                     TTIYRWV"
BASE COUNT          628 a          371 c          483 g          750 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttactggttc tgttgcaaag ttttaaatct actatcaaat
     2041 aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatcattt taaaggaaag
     2101 caatttcagc aggatgtgat tattgtagcc gtgggctact atcttcgtta taaccttagc
     2161 tatcgtgaag ttcgagaaat cttatatgat cgtggcatta acgtttctca tacgacgatt
     2221 tatcgttggg tg
//
LOCUS       DACDSY010000237         2232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDSY010000237 DACDSY010000000
VERSION     DACDSY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.242"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..544
                     /locus_tag="IWK44_002872"
     CDS             <1..544
                     /locus_tag="IWK44_002872"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9551323.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="IWK44_002873"
     CDS             complement(900..1472)
                     /locus_tag="IWK44_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9551324.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="IWK44_002874"
     CDS             complement(1488..2093)
                     /locus_tag="IWK44_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP9551325.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          700 a          454 c          338 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt ct
//
LOCUS       DACDSY010000238         2196 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDSY010000238 DACDSY010000000
VERSION     DACDSY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.243"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..561)
                     /locus_tag="IWK44_002875"
     CDS             complement(<1..561)
                     /locus_tag="IWK44_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9551326.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLL"
     gene            complement(563..1231)
                     /locus_tag="IWK44_002876"
     CDS             complement(563..1231)
                     /locus_tag="IWK44_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9551327.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1361..1945)
                     /locus_tag="IWK44_002877"
     CDS             complement(1361..1945)
                     /locus_tag="IWK44_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9551328.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          785 a          369 c          294 g          748 t
ORIGIN      
        1 aagtaagtca gcaagtttct tagtcgcttt gaagtccaaa tttgaagacg gttcatccaa
       61 tataattaat ttagagttta gcgataaact acatccaata gaaattttct gccgttcacc
      121 acttgatagc gaaaaaatgt ttttatctaa tatatttgaa agatctaata attcagctaa
      181 ttcattgatt cttttatcca tatgctcttt tgtaaatcca aagttttcca tcgaaaatac
      241 taactcactc gttgtatttg ttgtaaagaa ttgtcctcta ggatcttgac ttacgtagcc
      301 aatatttttt gatatacttt cttgtgttaa ggattgtagc gagttatttt caaataaaat
      361 ttccccaacc aattttccgt cataaagtct tggaataagt ccattcatta actttaatag
      421 tgtggatttc ccactcccag aagaaccagt aatcaatgta agactgcctt tttttattga
      481 aaaattaagg tcaacaagcg cttgttcctt aacatcctta tatgaaaaag aaacattgtt
      541 aaattcaaac aattctttca cctcaaatca aaatacttaa gaaaaaaata gtaacggaaa
      601 acaggatgat atagtctaat aagctcatgt ttgttttata gtaagaactt cttttcacag
      661 gagattctat cccctttgtt attgcattag cggtcatttc ttcagcaatc atagttgcac
      721 gtataagaac gggaactaac agaaattcca aatattgcag aggctgaaaa aaagacaagc
      781 gaatgtttct catttttaaa gattctttaa tcattcttat ttcagaacga taagtcggaa
      841 agaatctgat cactactgta atacttaaca ttattttttt cggtaaatgt attttttcta
      901 aagaggataa taattcattg ctagtataga cactaaccaa caaccatgag atcattacga
      961 ccggcaacat tcgaaataca atatataagg atgtcgcaaa aaatatagta aaaaactcta
     1021 tcttgatagc atcaataatt ttcattacta aaaacagaac tgaataaatc aagatgaaat
     1081 aaatacatgt ctttatttta cgaaacatca atactaaaac tgtagcaata atgaaacacc
     1141 ccacttgaaa tatatttccc tttcctaaaa aaataatgac cggaaaaaaa atatttaaag
     1201 taatcaaggt taacggattg atttttgaca ctctttcacc tccaaaaaat aagatcgcac
     1261 tgttatctta agctgataaa aatgcaccgt tattcagata acagtgcgat cttatcaaaa
     1321 agcataactg agaacaattc tcaatgcaag ctttttttaa ttaactcagt aattcaatcc
     1381 acccaggaac agaaaattga tacaatcttc tggttaatag catagtcttt tcttttgaat
     1441 agctatgctt caatatatca tagaatgtag cgtagaatga gtaaatgtag aagtggattt
     1501 cttcttcatt aaagactaaa ttttgattga tcattccgat agtctttaac ttatctagac
     1561 aacagactgt ctttttcgtc acaaatgatg taattttttc aacaatatct tgatatttgg
     1621 ttccagctga ttggaataaa atcaaatcga atatacgttt attctcaaaa atataattaa
     1681 taaatggatc taattcattg aagttctctc tccaactttt ctctatatct atattttcta
     1741 agtttaagag gtatttggta aactcttcat tatacatatt aataagcgca ttaatatgcg
     1801 gctgaataat ttcttcaaat aacgcttcct tagaagtaaa atgtttataa aaagcacccg
     1861 tagttacgcc tgcggtcagg cacagttgcc ttatcttagt ttgctgaaat ccttcttttt
     1921 caaaatggct tagtccactt tctattaact tttggtgagt tataatataa tctctggttt
     1981 ctatgggtac ttcctcctaa taagatgaat taactttatt atcttacaca ggacttgaag
     2041 tttcaatcgt taacgtttat tactgaactc attccttaga gctaaactaa tgagaaagag
     2101 cagaataact gatacaaaaa gcaatcctac aagaattaat agactcatct aaatcattca
     2161 ccttttctaa aataatccga gaaagattta aaaaaa
//
LOCUS       DACDSY010000239         2191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDSY010000239 DACDSY010000000
VERSION     DACDSY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.244"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..177
                     /locus_tag="IWK44_002878"
     CDS             <1..177
                     /locus_tag="IWK44_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551329.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="IWK44_002879"
     CDS             190..690
                     /locus_tag="IWK44_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9551330.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..>2191
                     /locus_tag="IWK44_002880"
     CDS             787..>2191
                     /locus_tag="IWK44_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP9551331.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVS"
BASE COUNT          722 a          291 c          485 g          693 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct g
//
LOCUS       DACDSY010000240         2156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDSY010000240 DACDSY010000000
VERSION     DACDSY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.245"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(33..266)
                     /locus_tag="IWK44_002881"
     CDS             complement(33..266)
                     /locus_tag="IWK44_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP9551332.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(281..1231)
                     /locus_tag="IWK44_002882"
     CDS             complement(281..1231)
                     /locus_tag="IWK44_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9551333.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1228..2070)
                     /locus_tag="IWK44_002883"
     CDS             complement(1228..2070)
                     /locus_tag="IWK44_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9551334.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(2072..>2156)
                     /locus_tag="IWK44_002884"
     CDS             complement(2072..>2156)
                     /locus_tag="IWK44_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9551335.1"
                     /translation="HGSVESVFNEENLKKAYGDTIFIGEGE"
BASE COUNT          712 a          408 c          256 g          780 t
ORIGIN      
        1 cattttctta tcctccctaa aaataaagtc ttttacttaa aacctttttt gtaatcgtca
       61 ataaattttt tccctttatc agttaattga aatcctatat ctaaatagtc ctcatatcta
      121 agtttagcca gcatctcact tacagatgct ggactaacac ttagaacttt tgctaaatca
      181 ttatttttta atttcttttt tctttttgat aattcctgaa ttgctagtaa ataaattagt
      241 atagtagtcg ttttaatttg taccatacct accctccaaa ttatttcgaa aggccttcag
      301 caattttatc caggttccat ttcatcatcg catagtaact atcaccatct tgtccttttt
      361 ctgcaattga atcagtaaaa atcgttgaat agataggaat attggtatct tttgatactg
      421 ttttcattgg tctatcatcc acactgcttt ctacgaataa ggagggaact tttgttgtgc
      481 gtaatttttc aactaagtgt tttatttgat ctggtgttcc ttcttcttca gtgttgattt
      541 cccaaatgta agcagaaggc acattatacg ctttcgagaa atatttaaag catccttcac
      601 ttgtaacaat cattttttta tcattcggaa ttgaagcaaa tttagattta gcttctttgt
      661 ctagagaatc caatttttca atatacttat ctagattttc tttatagaat tttttattat
      721 caggatcttt ttccgctaac tgtttttcaa tatttttagc gtaaataata ccgttttcta
      781 aatttaacca agcatgagga tcttccttcc ctttctcact ctgaccctct aagtaaataa
      841 catctatgcc atcacttgct gcaaaatagt ctttgttttc ctctttgttc gcatttttaa
      901 ctaattttgt aaaccaagca tttcctccag tttccaagtt aacaccgtta taaaaaatca
      961 aatctgcttt tgaagttttt tgaacatctt caggcaaagg ttcatattcg tggggatctt
     1021 tcccaatagg tacgatactg tgtaaatcaa ttttatcttt tgctatattt tcggtaatat
     1081 ctgctaaaat cgaattagta actactactt ttaatttttc gttcgaagcc actttatctt
     1141 tgtctttact atttattgaa catgccccga atataaaaag agcaatcaaa atccccagac
     1201 ttcccagtac tgctttccat tttctcatca taacgatctc tccttctttc ttaaaaattt
     1261 ttgttttggt gagattagaa aactaattac aaataagaat gccgctgtta aaacaatact
     1321 tgatcctgct gctatgttaa aactataacc aataaacaat ccaagcaatg atgaacttgc
     1381 tccgactgtt gcagaaataa atatcatagt tttcaagcta tttgcatata aatatgctgt
     1441 agctgctggt gtaattaaca ttgcaacaat taatatagtt cccacacttt gcatagctgt
     1501 tactgctact aaagttaata gaaccatcaa tagataatga taaaaactga cattcatccc
     1561 tattgctttt gccattaatg gatcaaaaga agtgattaat aattctttaa aaaatagaac
     1621 tactataacc aacactaaaa ccgaaacgcc aattgtaatc cacttatcaa tatcttgtac
     1681 agctaaaata tttccaaata aaatatgaaa taaatccgtt gagctatttg caactcctat
     1741 aaggataact cctagtgcta aaaaagaact gaaagtaata ccaattgctg tatctccttt
     1801 tattacacta ttgcttttta taaaagtaat aatgatagaa gctaatagac caaatacaat
     1861 cgcaccaata aaaaaattaa tacctaaaat ataagaaatt gctactcctg gcaaaacagc
     1921 atgagaaata gcatcaccca taagagacat tcctcttaaa ataataaaac aacctactgt
     1981 tccagaaaca atccctataa ctacagatgt aattagagca ttctgtaaaa agtgaaagtc
     2041 atacagacca ttaataaaat tttgaagcat attattcccc ctctccaata aatatagtat
     2101 ctccataggc tttttttaaa ttttcttcat taaatacaga ttctacactt ccgtga
//
LOCUS       DACDSY010000241         2137 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDSY010000241 DACDSY010000000
VERSION     DACDSY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.246"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..518
                     /locus_tag="IWK44_002885"
     CDS             <1..518
                     /locus_tag="IWK44_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551336.1"
                     /translation="EKIHFTYQYFSYEWVKICFNEKEAKRVFPQNSRSELSKEKQLIL
                     DEAHEVLQGLSWQGTYLMTEKELINTVCERLRNVGDNSVKNALIKNRGYFVKQGFTLE
                     RASKDIKKLYNVKASSSFLIYLAKGAGIDETIKELILSGAGIVPLYQFTSVTRDDGRR
                     VRKRKDPIVLN"
     gene            complement(594..929)
                     /locus_tag="IWK44_002886"
     CDS             complement(594..929)
                     /locus_tag="IWK44_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9551337.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            1056..1271
                     /locus_tag="IWK44_002887"
     CDS             1056..1271
                     /locus_tag="IWK44_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9551338.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            1308..1670
                     /locus_tag="IWK44_002888"
     CDS             1308..1670
                     /locus_tag="IWK44_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP9551339.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            1716..2042
                     /locus_tag="IWK44_002889"
     CDS             1716..2042
                     /locus_tag="IWK44_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551340.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT          753 a          295 c          436 g          653 t
ORIGIN      
        1 tggaaaaaat tcattttaca tatcaatatt tttcttatga atgggttaag atatgtttta
       61 acgaaaaaga ggctaaaaga gtatttcctc aaaattcccg atcagaactc agcaaggaaa
      121 aacaattgat tttagatgaa gcacatgaag ttttacaggg cttatcgtgg caagggacat
      181 atctaatgac agagaaagaa cttattaaca ctgtttgtga acgcctacgt aatgttgggg
      241 ataacagcgt gaaaaatgct ttaataaaaa acagaggcta ctttgttaaa caaggattca
      301 ctcttgaacg agcttctaag gatattaaga aactatacaa tgtgaaagca agtagtagtt
      361 ttttaatata tttagcgaaa ggggcaggga tagatgaaac aataaaagaa ttgatattat
      421 caggagctgg gatagttcct ctgtatcaat ttacaagtgt aacaagagat gatggacgga
      481 gagtaaggaa gagaaaagac ccaattgtat taaactagac aggttatcca tagctcttaa
      541 tcttatttca tctcaataca cccaccgtta aagttcggtg ggttatttat ttttcaatct
      601 ttcaatagct gattgcgttc attttcttgc ctttttaata gcttcatata gtcaattgtt
      661 cgaactgcat cttcagtgct aagagaacga tagagagcca aaaactcttc ctcataatct
      721 acgacaacat tgtctagaaa atggcttaca ggggttccta gggctctaga aagagctgtt
      781 aagctatcga ggttaggctc tgttacagaa ttttcccatc ttgctactgc ctgttgtgta
      841 gagaagattt tttcagctaa ttctttttgt gtccacccga gagcttttcg cttttctcta
      901 atgacctttg catattgttt ctgtttcatt aatacacccc catctcagta tagtacaaca
      961 agttgttgta tttgtaatgg tatttgaagt tttttatatt ttatgagttg aaatacatca
     1021 tattgttgta tataataaaa caggagggga gaatcatgaa aaaagtaagt aatctcagcc
     1081 gatacagaaa agaaaaagga ctttctcaaa cggagttagc aaaaaaaatg aatgttacac
     1141 agcaatgtgt aagttcgtgg caaacaggga gaacgattcc taaaccctat caaatgaaga
     1201 tgctttctga gatattaagt gtaccaatga atgagctgtt ttctgatgta tttaataaag
     1261 taaacagtta actaataagc aattgaatga ttttaggagg agaagacatg tattgtagaa
     1321 aatgtggcaa agaaataaca gatgaaagta agtattgcca gttttgtgga gcactacaag
     1381 aaacagatga gacaaccgga acagacacag taaagaaaag tactgagcag acaattaatg
     1441 aaatgaagga acaaatcgta catggacaag agacagggaa gctgtatttg attattggtt
     1501 ggatttctat ggtagtatcg ttagcattta ttcctgttgt atttggtgct gttgctgtta
     1561 ttatgggcta cttatatcga gaaaaagatg agaaaatggg tacgattttg atgatagcag
     1621 gggtagctgg agcaatcttt ggtgtacttt tagggatgtc aatgtactga taagagggat
     1681 aagagcaaat tattttggag ataggagata aaaagttgaa taaagtaaat atgaacaaga
     1741 agtttgtcat tataggtatt gttgtcttgg ctattatagg tattattttt tcaaaaatga
     1801 caggtacatc tggattagag aagtcatcaa aaccattggt aacacagatt ttacaagaac
     1861 agtacaactt aaatcgatca tgtgatgatg tgacgattac tcatgaaaat ggagataaca
     1921 cataccgggc taaagctata ttagacaatg gaagtgctat caacataaat attgagtact
     1981 atccaaagaa agaccgtata tatgtggaga taccatatgc tgaggtgtta atgttaaatt
     2041 gatttaaatg tattatgcat accgtccaaa caggaactcc caaaggtgta aattcactag
     2101 ggagttttta tagtttccta taaattaaat actaata
//
LOCUS       DACDSY010000242         2104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDSY010000242 DACDSY010000000
VERSION     DACDSY010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.247"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            169..1539
                     /locus_tag="IWK44_002890"
     CDS             169..1539
                     /locus_tag="IWK44_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAP9551341.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="IWK44_002891"
     CDS             1560..1844
                     /locus_tag="IWK44_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551342.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          731 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cata
//
LOCUS       DACDSY010000243         2083 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDSY010000243 DACDSY010000000
VERSION     DACDSY010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.248"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            363..889
                     /locus_tag="IWK44_002892"
                     /pseudo
     CDS             363..889
                     /locus_tag="IWK44_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1044..1619
                     /locus_tag="IWK44_002893"
     CDS             1044..1619
                     /locus_tag="IWK44_002893"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9551343.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1662..1829)
                     /locus_tag="IWK44_002894"
     CDS             complement(1662..1829)
                     /locus_tag="IWK44_002894"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551344.1"
                     /translation="MENTFELYNKTTVDKLIPILVPTDLNGHDVLLRFNGFLGEDYRY
                     LKISDPQPLTE"
     gene            1846..2028
                     /locus_tag="IWK44_002895"
                     /pseudo
     CDS             1846..2028
                     /locus_tag="IWK44_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          737 a          337 c          392 g          617 t
ORIGIN      
        1 tacaagacaa agtatagcaa gcaaaattct tctccacttc cagaattttt tattttcatt
       61 tttagaaaag tgaaaaaact gacaacaaaa gcttgtttct ttcactctat taatatcttt
      121 agtgaaaaag ctcgttttat atcgtattct tccagcgaat gtacttctca taagtccaaa
      181 aaaagctgcg aaaaagaaag tacctcggta cctacttttt cgtagctgca cataacttga
      241 gcaagtatta atcgaagata cttttgttga gagtgtaaaa tattttgtgt aaatagaaaa
      301 aaggaagtcc cttctgtaga atagagttac cacaacacat tcacagaaaa gaggacttcc
      361 atatgaacga ttttactaca gaaatcctaa agactctagc gaacaaaggc gatttgaatg
      421 gattattccg tgtccatttg gaaaaagctg tcaatacgct tctcaaaacg gagttaacgg
      481 ctttcctcga ttacgaaaag tacgatcgca ttggttttaa cacgggtaat tctcgtaacg
      541 gctcctatga ccgtacggtc aagaccgagt atggggaact tcatctccag attccgcgcg
      601 accgcaatgg cgagttcaag caacagactg ttcctgctta tagacggacg aatgacacat
      661 tagaggagac cgtcattcac ctcttccgaa aaggtattac catgtcggaa atcgcagact
      721 tgattgagaa aatgtatggg catcactaca cgccccaaac catgtccaac atgactaaag
      781 tgctgactga agaagtaaat gcatttaaag ttagaactct aaatgataag tatgtcgcta
      841 ttttcatgat tccttcagtt tttcagatga tataccactc aaaaaacaat atgagagatg
      901 ttcaggaata ccatctctaa tggcttataa attggtttta gggatagtag atgttataga
      961 catatcaagg aataaacgag cagctgcata aaagcagatg gaattaagga ataattgaat
     1021 tgtaaaagaa gggaataaat ttaatggcac aattatttta cagatatgga acaatgaata
     1081 gtggaaagag tattgagcta ctgaaagtgg ctcataatta tgaagaacaa gggaagagag
     1141 ttatcataat gacaagcgca atagatactc gatcaggatt tggaaaagtt gagagtcgta
     1201 taggtgtttc gaaaccagct attgcaatta cttctgaaat ggatgtgtac ggagaattag
     1261 tggagattat tgatcagtac ggaaaagttg attgtatttt ggtcgatgag tctcaatttt
     1321 taagtagaga gaatattatt gatctatcac ttatcgtaat gaatgaggaa gtaccagtaa
     1381 tttgctatgg attgaagaat gattttcaaa ataatctctt caagggatcc gaagcattat
     1441 tgacatttgc tgataaaatt gaggaaatta aaactatttg ccatttttgc aataaaaagg
     1501 ctgtaatgaa tgctcgtatt gttgatggta ttccagttta ttcaggtgaa cagattgtag
     1561 taggaggtaa tgaagattac ctttcggtat gtcgcaacca ctactacaac gaagattgaa
     1621 ataaaaaacg tgtaatcaaa taattgatta cacgtttttt attattctgt taaaggttga
     1681 ggatcagaaa tttttaggta tctataatcc tcacctaaaa atccgttaaa acgaagtaaa
     1741 acatcatgac catttaaatc agttggaact aaaattggaa ttaatttatc aacagtagtt
     1801 ttgttgtaaa gttcaaaagt gttctccatt ccttctggtt ctacaatcga atctttcaat
     1861 aaacaaatca agaaatacag ccgcagaaaa gagcagtttc aaaatgaaga atcactagag
     1921 cgtttcctag tatccatctt tgatacatac aatcaaaaat tcttaaatag aagccataaa
     1981 ggcttccaac aagtgacgga tacattagct tcaatgttta ctgagtaacc cattattttg
     2041 cagaaggacg agttatttac acaaaattat tgacgctccc tgt
//
LOCUS       DACDSY010000244         2070 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDSY010000244 DACDSY010000000
VERSION     DACDSY010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.249"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            8..526
                     /locus_tag="IWK44_002896"
     CDS             8..526
                     /locus_tag="IWK44_002896"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9551345.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            654..1337
                     /locus_tag="IWK44_002897"
     CDS             654..1337
                     /locus_tag="IWK44_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP9551346.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1646..1719
                     /locus_tag="IWK44_002898"
     tRNA            1646..1719
                     /locus_tag="IWK44_002898"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1680..1682,aa:Arg,seq:tct)
     gene            1878..>2070
                     /locus_tag="IWK44_002899"
     CDS             1878..>2070
                     /locus_tag="IWK44_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP9551347.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGV"
BASE COUNT          701 a          322 c          423 g          624 t
ORIGIN      
        1 gaaaacgata cctgattaca aaaaaataac ttttataagt tctataacgt tgattatatt
       61 tttaattctt attcttctcg tcgtcttggt tccccgaatt tttggtttga ctccttatat
      121 tattgaggac aaagaaatga gtccatatta tgcacaagga aatctgatat atgtacgacc
      181 gaaaaataca aatgagattt tagtcggaga tattattaca tattatgaaa attcaggaaa
      241 aaaaatctcg acgcgtcgcg taatagccgt tgaaaacaac cacgaagatt tttatacaaa
      301 agctgataca aagacttata ttgagcctgg agtagtaaaa aagagaaata ttatcggtag
      361 tcccatcttt cagattccat atattggact agtcctttca aaaacggcat ttgcatggat
      421 tcaggggtta ttctttacga ttgctttttg cctaacagga atcacagcat ggaatacctt
      481 tgtagagttg aaaaagaagc ggagacgaac acagaaattt ttgtaagaat gttaaaaaca
      541 taaatgtatt ccagagatcc aacgactctt ctgaacatga aacacgagaa aacactcatg
      601 cagcttgtag atttagtcag ttgttcgtat aatgtgtgaa gaggaggagt agtatgcgca
      661 atgaaatgat gcatcgtcct gtagtcaaag aagaactatt ggattttatg cgtacccagc
      721 aaaaaaaatt accgggtgag ctaggcaaac tggaagaaga agctcatgta gcagaagtac
      781 cagttattcc ccatgaaaca gttgtatttt tgaaattttt acttggacag cttcagcctg
      841 aacgaatctt ggagatcgga gcagctatcg gtttttcatc aagtgtcatg gccacgacaa
      901 tcggagaaaa cgctcatgtt acgacaattg atcgtttcga tgtcatgatc gagaaagcta
      961 aaaaaaacta tgaacgtcta ggactgacag ataaagtgac tttgcttgaa ggtcaagcag
     1021 cagatatctt gccggagttg tctggtccgt atgactttat ttttatggat agtgcaaaat
     1081 caaaatatat tgaattttta cctgaatgct tacgtttgtt aagaaaaggt ggcgttttga
     1141 tggtagatga catcttccaa ggaggaacga tcttgctccc agatgaagag attccccgtg
     1201 gcaaacgagc tatccatcgt aaattaaatg aatttcttcg tgttgtgatg gatcatcccg
     1261 atttgacatc gacaatcttg ccattaggtg atggtgtgat cttaatgaca aaggaatcag
     1321 aaacaatcga gttgtgatac atgattatgg cagcctgtag ataatcgatt gaatcgaggg
     1381 ctcaattcga acttgatgta aaagtttcta tatttgtaag tcaattaaag gatacaggaa
     1441 atctaaataa acaggagtag catgaagtca atgtagctcc tgttttcaat tgataacctt
     1501 agaacagctg aaggagaatg tctcgtttta aaagattgat ttagtagttg ggccaaaata
     1561 attcattatt tgctaattaa ataactattt cgtattgaca gaagcaattt aattatctat
     1621 aattattgat gttccgctga ttcttgtcat agtagctcag ctggatagag catccgcctt
     1681 ctaagcggac ggtcgggggt tcgaatccct cctgtgacgc taaaaaaccg ttagaaactg
     1741 aggtttctaa cggtttttct ttgttttttg tgccaatatt gtttgttttc aaaggcaaat
     1801 aaaataaaaa aacatcctta tcgagtacaa ttataaggag caaaaaaata aaactcatca
     1861 gaaaagagga agaagatatg acgatactat ctagaatttt agtgactcta gttgcaattg
     1921 aattttttta tatcatgtat atcgaaacgg tgagaactga ttctgacacg acaagtaggg
     1981 tttttaaaat gagtaaagaa gagttaagca gaaaatctgt tcaaacattg tttaagaacc
     2041 aaggagtgta caatggacta ttaggagtgg
//
LOCUS       DACDSY010000245         2059 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDSY010000245 DACDSY010000000
VERSION     DACDSY010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.250"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..223)
                     /locus_tag="IWK44_002900"
     CDS             complement(<1..223)
                     /locus_tag="IWK44_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551348.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLA"
     gene            537..1151
                     /locus_tag="IWK44_002901"
     CDS             537..1151
                     /locus_tag="IWK44_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9551349.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(1175..1525)
                     /locus_tag="IWK44_002902"
     CDS             complement(1175..1525)
                     /locus_tag="IWK44_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9551350.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1519..1782)
                     /locus_tag="IWK44_002903"
     CDS             complement(1519..1782)
                     /locus_tag="IWK44_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP9551351.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          711 a          347 c          307 g          694 t
ORIGIN      
        1 gagcaagtaa tggagaaact attaaataat tttcagtaac agttaagtcg ttagttgggt
       61 taccaatggc tattccgttc ctatctaaag gagttaattc ttggttatta tctactacag
      121 tagttggttc atttgtacta tcagcaaatg taggaattgt agttccagtt acactaattc
      181 caatagttaa aatacctaaa gataaaataa acttattttt cattgtctat cacctcgaaa
      241 tcaaaaataa gaaaacgttt tcttaataaa tcttaacaga taaatcaagt tatatactag
      301 aaaattcata aaatatattt ttaatgtcat aatcgtttaa attttcttga ttgacattta
      361 tttacaaaaa atattgccat ttaaaaagaa aagtgacaca tactatttcc tccatcatta
      421 gtttttgtct atataaggta taccctaact agacacgacc gcgacattga aaaatctggt
      481 tttctcgtgt atgattaggg tgttttttaa atagttgcag aaagtaggta aaaattttga
      541 gagttgcata catacgtgtc tcatccatcg atcaaaatga acaacggcag attgaagaaa
      601 tgaaaaaatt cggtgcagag agaattttca ttgagaaaca atctggcgca acgattactc
      661 atcgacctgt ttttcaagaa gcactggatt tcgtccgaga tcaagatatt tttattgtgg
      721 aagctattga tcgacttgga agaaattacg acgagatcat cgcttcagtc aattacttga
      781 agaagaaaaa tgtgcagctg atcatcacaa gtctaccgat catggccgaa gcaattggca
      841 atcctctcct ggataaattc attaaagatt tgatcattca aattttggcc atgatcgctg
      901 aacaagaacg taccgaatct aaacgacgtc aggcacaagg cattaagatt gcaaaagcga
      961 acggtgttta taaaggacgt cctaagctat acagtgccaa tgcaaaagat ccccaacgtc
     1021 gtcttgttta taaaaacatc gttgaggatc taaaaaaagg tgttgcgatc gctaagattg
     1081 caaaagacta caatgttacg agacaaaccg tatatagaat aaaaaaagac agcatggtca
     1141 atcacgaata gttgatcatg ctgctgtttc tattttattt gcttactttt tgaaaaactg
     1201 ttctaacttt catcaataac tcctcaacta aactatcatg cgctttttcc aaaaaaatac
     1261 actctctggc ttcataatca attgtcgtta gttgatctaa taatactttt cctgttgtct
     1321 tcatttcata ttctgagttt atatttacat atagcggata gtttctagtt gtactagaaa
     1381 ttggagcaac tgtaacaacg ttactataat ccgcaataat atcatgactt aaaacaatat
     1441 aaggtctttt acctgtctgt tcgtgtccag atcgaggtgc tgtattcaat aatagaatat
     1501 cgccttgtct aggttttctc accatttttc attacctact gcttcaccta aattttgaat
     1561 cgtcacatga tttctttcat cagtgtaatt ttcaaataac tgttcaattg ttaattcatt
     1621 ttctatatta gccgcttcga taattagccg cttctgaccg tttacttctt cttgtattat
     1681 cttcactttg tcttctgtat gaattccaag atattccata atatactttg gcaatctaag
     1741 gccattacta tttccccatt tttttatagg taacatttcc atagtagaca cctctttctg
     1801 tatatacata gtataacata cataagaaaa aacgtatata cttaattatc cttttctatt
     1861 aaattagatt aacattccat gaatccataa actatgttaa catttatatg aaaatagtcg
     1921 aaaggaaatc ataatatgtt taagaaaaca aaatctttag aatcaggtaa gaaaaaaatt
     1981 gatctaaaaa taatctcttc tggatacttt aaagatttca tagttattga tacaaaatgg
     2041 aataaaaagg acatagaaa
//
LOCUS       DACDSY010000246         2059 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDSY010000246 DACDSY010000000
VERSION     DACDSY010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.251"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..337)
                     /locus_tag="IWK44_002904"
     CDS             complement(<1..337)
                     /locus_tag="IWK44_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9551352.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="IWK44_002905"
     CDS             complement(404..559)
                     /locus_tag="IWK44_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9551353.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1177..1398
                     /locus_tag="IWK44_002906"
     CDS             1177..1398
                     /locus_tag="IWK44_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551354.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1399..1656
                     /locus_tag="IWK44_002907"
     CDS             1399..1656
                     /locus_tag="IWK44_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551355.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1792..>2059)
                     /locus_tag="IWK44_002908"
     CDS             complement(1792..>2059)
                     /locus_tag="IWK44_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9551356.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          679 a          462 c          314 g          604 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aaacgctgaa acccttggga gagtaaggct
      721 aaacagctat cgaccaatga ccaatgacca ataagggggg tgtcgtagtc cccccctcct
      781 ccgcccaatt cataaacacc aaaaccaaaa aacaaacctc agggctccgc cccgaacccc
      841 gacctccttc taccgaaata tatgcaggcg ccacgcttac tatttcgttc agctatcggc
      901 ttttgcaatc taacacgacc gtttcgctac cagtcgcctt acaggctcca ttctcgcttc
      961 tcttacgtgt tttaacgatt gcgccgataa ctaaacgaaa tagacgctaa atcgcttaaa
     1021 acgcaaaata gacccttttt agagcattga ttttttctac tagtcatttc gataatataa
     1081 aattgaccac aacacagaaa acttatatta taagcttatg tcgtaagtcc gacgtactca
     1141 cgtaatatag acggacgtta aatggaagga gcataaatga gagtgataaa atcagaaaaa
     1201 ttcactttgg ccgaaattat gacgaccagc gaacttgcac aagaacttga ttacaaccaa
     1261 gcttacgttt tgcgcttagc gaaagaacat ctgactgaag gaaaagaata tcgctctgcc
     1321 ggtagaagaa actatctttt cagcccagaa gcccttaaaa aactagaaaa agtcataacc
     1381 accaaagaag gtgagtaaat gcctaaagaa acaactatca acgttgaaca actagaaaaa
     1441 ttgctagaat catattccaa tgattttatc gaattgaccg gcggaaaagt cgaagaaacc
     1501 accgacctct ttgacctact tctcacaaat attctggaat taaactctga agaacaaaaa
     1561 gagcataagg gaaaactgat agcaccttcc tacaggaagg tggctaagtc ggctgtgcca
     1621 actggttttc ttacaaaaca accttatatt ttttgatatc acaaaaaaat ccattttgga
     1681 cctattttcg gtcataatat agtacctact tttggtcata gtttcgtccg tagttttggt
     1741 catagtgact aagactttca cgcacaaaaa aagagctgaa attaacagct cttatccttt
     1801 atttattttt gaatttacac ttccaccatg acgattcgat tttatatatt gcccgataat
     1861 ataagctgaa ttatcacaaa gaagcggaaa ccaatcatca aatctttctg acattgcata
     1921 gtcaattaaa tctttgattt cagtgatatt cttagaatca acaaacgaca tcatctctct
     1981 aatcaactca tatcgttctg cactcgttac tgacaaataa ctagcaattt ctgcaccacc
     2041 atgagcgata atatcactt
//
LOCUS       DACDSY010000247         2057 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDSY010000247 DACDSY010000000
VERSION     DACDSY010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.252"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..463)
                     /locus_tag="IWK44_002909"
     CDS             complement(<1..463)
                     /locus_tag="IWK44_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9551357.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESS"
     gene            798..1232
                     /locus_tag="IWK44_002910"
     CDS             798..1232
                     /locus_tag="IWK44_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9551358.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            1255..1575
                     /locus_tag="IWK44_002911"
     CDS             1255..1575
                     /locus_tag="IWK44_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551359.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1716..1907
                     /locus_tag="IWK44_002912"
     CDS             1716..1907
                     /locus_tag="IWK44_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551360.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1978..>2057)
                     /locus_tag="IWK44_002913"
     CDS             complement(1978..>2057)
                     /locus_tag="IWK44_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP9551361.1"
                     /translation="THIIELTLSVIFLIIWIYFLYKNQN"
BASE COUNT          726 a          317 c          448 g          566 t
ORIGIN      
        1 cgcttgattc tttttgtgcg atccattctt ttgcagagct tgtacttgct ggagtagcag
       61 cagtttctgt tacagcagga gcaggttgtg tttcagcaac aggcgctgct tgtgtttccg
      121 tttgaaccgt ttcttgtaca acaggttgtt cagcttgaac aggttcagct tggactggtg
      181 cttcttgaac ggctgcttga ggttcagcag ctgcttgtgc agagccttct gttgggattg
      241 ttaattgttg tcctgagtag atcaggttaa tatcagaaat tgaatttgat tctgcaatgg
      301 cattgatcaa ggagttatca ccaacgtatt tttgagaaat cgttgataga gtatcacctg
      361 attgaacggt ataagcttcg tccgcatgag cagtcgttcc catgaagaat gcagcgccgg
      421 cagccaaagt tgttccaaaa agtaaagttt taagtgatgt catgaaaaat ctcctttatt
      481 cgttaaaaag ttttatttgt tgtgttcaac gaggaatact ttaacacgtg aaaatatttc
      541 ataggtaaaa gaagcatgac aatttatgac aaatccacgt tttttgtaac agaatgatgt
      601 tcgctcgatg tgaaaaatga tgaggaattt cacataatgc agaaataatg ggattagaac
      661 aggtgttctg atgagaaaag aaatgtataa ataacttgaa agaaattgct tttgttcatc
      721 attttattta catattgcgt atattttttg tatttttgct tgagatgtta caataaaaga
      781 aaaaggaggc ggttccaatg agtaaaagat accagaacat catggtagca gttgacggat
      841 caagacaatc gatcaaagcg ttcgctgaag cccttgactt agcaaaggac aatgaagccc
      901 acttatttat cgtgtcaatc atcaacaaag ttgagctgac ccatagtgcc tatgcctttt
      961 caaaaatcta tgcagatgaa aaacaaagaa cagaagtagc catgctgaaa aaaatcaatg
     1021 atgccaaaga gtacggcatt gctgagatcc acgcaatagt tgaaactgga gatccaagaa
     1081 atttgattgc caatgtcatc cctggacaag aaaatattga tttgatcgtt atgggagcga
     1141 caggaaaagg tgctatacaa caagcattag ttggatcgac cgcttcttat gtcgtatcac
     1201 atgccccttg cagtgtgttg gtcgtgaagt aatgagataa gggaggaata gaaaatggtt
     1261 cgtcctcaag aagtccgtgc gccaaaagaa aaaatagaaa ttttagcaat tattgaagac
     1321 ggcacacaaa caaagaaggg atactcgatc gctttagtaa agtggaaagg aaaaaaaggt
     1381 gttgctctga gatgggatgg agataaccaa caagataaag gattccctgt cacagctaac
     1441 ggttatcatc cagcgtggtt tgttctgcct gataaattta tggaattata cagctatgat
     1501 tatgcaagag ccgcagcatc aatcaaagca ttagataaat taacagaaga gcagaatgaa
     1561 atggaggaaa aataaaaaag acgaaccaat cagcctttag aaaactgatt agttcgagga
     1621 gtaaaaatga aaaaaggtta tttgggatga aactagaata atgcgtataa cttaaataaa
     1681 acttaaagca ataaaatggc aagagggtga ggcaaatgga agcaggaaaa gtattacagg
     1741 aagtaaaagc actatgtgca gataaaaaat atgaagaagc gaaaagcctg atcgaaaaga
     1801 acaaagaaaa tttggaagaa aagtacccga tcgcacaaga atttatagaa ttaaaacaag
     1861 gaacgatcct tcaccgattc aaacattttt ttggtataac agaatagaaa aaacgattaa
     1921 agatcggcag aaatttgctg catctttaat cgttttcttt ttaaacatgc gagggattca
     1981 attctgattc ttatataaaa aatagatcca aataataaga aaaattactg aaagcgtcaa
     2041 ttcgatgatg tgtgtta
//
LOCUS       DACDSY010000248         2001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDSY010000248 DACDSY010000000
VERSION     DACDSY010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.253"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            174..1310
                     /locus_tag="IWK44_002914"
     CDS             174..1310
                     /locus_tag="IWK44_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9551362.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1447..1791
                     /locus_tag="IWK44_002915"
     CDS             1447..1791
                     /locus_tag="IWK44_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP9551363.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT          697 a          343 c          417 g          544 t
ORIGIN      
        1 aaagaagttg tgacagaagt gagcagcttc aaggagctgc gacaagactt ttgtcacagc
       61 tccttttttc tatgcccatg aaacgtcctc tagacaaaaa tgagctcttc tttggtttgt
      121 tcgctatttt atgctagaat gtgcctagca ataatttgga agggatgaat gaagtgaaaa
      181 ctatttatct ggatcatgcg gcaactaccc ccatgcatcc acaagtaatt gaagcgatga
      241 cggcaatcat gcaagacacg tttggaaatc cttcaagcat acatggattt ggtcgccacg
      301 cgcatgaaaa attggaaaca gcaagacaaa tcattgcaga cagtctgcaa gcaaaacctc
      361 acgagatcat ctttaacagt gggggaacag aaggggataa tacagctatc ttagaaacag
      421 cgttctcgcg tcaaaaagaa ggcagacatc taatcactac agctatcgaa catcctgctg
      481 ttcttaatac catgaaatat ttagagacaa aggggtttga cgttacatat ctgcctgtag
      541 atgaaaaagg gaatctgtcg atcgaagagg tcaaaaaagc gttacgagaa gatacgatct
      601 tggtctctgt gatgtacggg aacaatgaaa tcggaaattt gatgccaatc aaagaaatcg
      661 gtgaactttt agcggatcat ccagcttatt tccatacaga tgcggtacaa gcgtacggaa
      721 accaaacgat ccatccagaa gaattaggaa tcgatttatt aagtatctct gctcataaaa
      781 tcaatgggcc aaaaggcgtc ggtttcttat ataaacgaga cggagtaaat atccctgcat
      841 tgcttttagg cggggagcag gaagaaaagc ggcgtgcagg tacagaaaat ttagcaggga
      901 tctggggtat ggcaaaagca gttgaactgc tgtcacctga agaacgtgaa gcacgtaaca
      961 acaaatataa aggattccag caattgattc ttgatacatt agaagcaaac gaaatccctt
     1021 atcaagtaaa tggtgattta acgaataaac tccctcatgt gttgaatctt cgcttaattg
     1081 gcgaagcaaa tgatttgcta ctgatgaagc ttgatcttaa tggggtcgct atttctactg
     1141 gttcagcttg tacagcagga aatatcgagc cttcacacgt attggaagca atgtatggag
     1201 aagatacacc gatactaaaa gaatccatcc gtatcagctt tggtttggga aataccgaag
     1261 acgacatcca gtatttcctt gatcaattag ttcgtgctgt caaaaaataa ctagtgaagt
     1321 cattcttata tgaacagatg tctgagagaa cgccttcatt ttttagggaa cttaactgaa
     1381 aaacctagta aaaatcagga taaactttta taataaagag aaactaattt tatcgaggtg
     1441 aaaatcatgg catttgaaac aacagcaacg gtactgggcg caccaattag ctatcgtttg
     1501 catcctgatg caaaaagata tacattgcgc gataatggat ttacagaaac aaacggaggc
     1561 aattatcaat tgatccgacc attagatgcc acgccgcaaa gcaaagaagg attcaaatta
     1621 aaaatcactg tatcaaaaga tattcaaaca ttgaaaatgt cgatcactac agcaaatgga
     1681 ttgaaagcag tcaatatttt caaagatcca aaacagaaaa tgagccaaga taaattttat
     1741 tttctgatgg atgggatgat tagccgtggg ttattcgaaa aagtcgaatg atctgtggat
     1801 ataaaaagac aactagtttt gaaacggcct attgttagat gactaacttt gtaaggacga
     1861 cgcaaattag ttgtcttttt tatttttaaa tttaacacat gttgttattt ttaatatttt
     1921 gctgatttat tgtttctaag taaagaaatt taaattgaaa cgattgttat ttagggaaaa
     1981 aggtattttt tgaaaaaaga a
//
LOCUS       DACDSY010000249         1991 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDSY010000249 DACDSY010000000
VERSION     DACDSY010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.254"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..153
                     /locus_tag="IWK44_002916"
     CDS             <1..153
                     /locus_tag="IWK44_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9551364.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="IWK44_002917"
     CDS             159..575
                     /locus_tag="IWK44_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551365.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="IWK44_002918"
     CDS             681..944
                     /locus_tag="IWK44_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9551366.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..1216
                     /locus_tag="IWK44_002919"
     CDS             944..1216
                     /locus_tag="IWK44_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9551367.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            1293..1622
                     /locus_tag="IWK44_002920"
     CDS             1293..1622
                     /locus_tag="IWK44_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9551368.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1612..1899
                     /locus_tag="IWK44_002921"
     CDS             1612..1899
                     /locus_tag="IWK44_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9551369.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          747 a          251 c          395 g          598 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggtagatg aagatgaaca cgtggttgaa atttattcag caaggacgca
     1201 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
     1261 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
     1321 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
     1381 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
     1441 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
     1501 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
     1561 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
     1621 gaaatcgttc cttcaaaaca tgtcattaag tatttaaaaa agctaaaaga gaagccattg
     1681 aaagaaaaat ttttgaattt aatttatgat gaaattgcga ttgatccata taagggtgaa
     1741 caaaaaactg gggatttatc aggtatttgg tctagtgggt tcaaatatgc gggcacaaca
     1801 tatagagtag cttacgagat tatagaaaac aaggttattc cagtattgtt atgtggaacg
     1861 catgaaaact tttatgaaca gttgaaaaaa ttaatctagt gatttgttgt cgttcttaag
     1921 aaggacggct tctttttatc gaaaatctga ttcgtctaca tgactaaagt cgcgagtatt
     1981 ctctgcttct c
//
LOCUS       DACDSY010000250         1990 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDSY010000250 DACDSY010000000
VERSION     DACDSY010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.255"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IWK44_002922"
     CDS             complement(<1..914)
                     /locus_tag="IWK44_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP9551370.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IWK44_002923"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IWK44_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP9551371.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          656 a          366 c          389 g          579 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt
//
LOCUS       DACDSY010000251         1989 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.256, whole genome shotgun sequence.
ACCESSION   DACDSY010000251 DACDSY010000000
VERSION     DACDSY010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.256"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            131..820
                     /locus_tag="IWK44_002924"
     CDS             131..820
                     /locus_tag="IWK44_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9551372.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            834..>1989
                     /locus_tag="IWK44_002925"
     CDS             834..>1989
                     /locus_tag="IWK44_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9551373.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYF"
BASE COUNT          619 a          275 c          400 g          695 t
ORIGIN      
        1 cattatactc caaaataata aaaaaaactc ctaaatactc catttttatc tttactttta
       61 tgaaagcgga tgctatattg tttttggagt tgttatccaa aaataataaa aaattggagt
      121 gtttaggata atggataaaa agaggttttt agatcttatt tcaaaaggtt tgattgtttc
      181 ctgtcaagca ttacctggtg aaccatttta caaagaggaa gaaagtttaa tgccttattt
      241 tgctttggct gctcaaaggg ctggagcagt tggtatacgt gcaaattctg ttcgagatat
      301 tattgaaatt agaaagtgtg tatcattacc aatgattgga attattaaaa gagattatcc
      361 accagaatct ccattcatta ctgccactat gagagaagta gatgaattag tagaaaccgg
      421 tgttgaggta atcgctcttg attgcactct tagaaatcga catgatggac tatctataaa
      481 ttcttttatt actaaaataa aagaaaaata tcctcatcag ttatttatgg cagatatttc
      541 tacatttgaa gaaggcaaaa atgcatataa agcaggaata gattttgtag gaactacttt
      601 aagtggatat actgaggaaa gtaaaaaaca agatgggcca gatatagatt tgattaaaaa
      661 attggtagaa gaagggattt gtgtaattgc tgagggaagg attcatactc cagaacaagc
      721 aaaagagata aaaaaaatag gagttgcagg tattgttgtg ggtggtgcta ttactcgacc
      781 acaggaaatt gcatcaagat ttgttaaagc attaagttag ggggagaaat aaaatgttta
      841 aaaaattttc acaattagga cgcgctttca tgttaccaat tgctatatta ccagtagccg
      901 gattactatt gggactggga ggagcgttaa caaatgagag tgctataaat gcttatccat
      961 ttttagatca accgtggttg catacaattt taagtattat gagttatgct ggaaatgcag
     1021 tttttgcaaa cctagcacta atttttgcaa ttggtattgc tgtaggactt gcaaatggag
     1081 ataaaggaac agctggttta gctggtggag tagcttatct agtatatact gcaactatta
     1141 gtgggtttct tgaacttttt tcagcaaaag atgcaacact agatacaggt gtagtgggat
     1201 caattgttgt tggtggtact gttgcatttt tacacaatcg ttatagaaaa attgaattac
     1261 cacaattctt aggttttttt ggaggatcaa gatttattcc gattgtttct agtttagctg
     1321 caatcattat tggttctttc ttttatttaa tttggccacc aattcaaaat ggtttagtag
     1381 ttgctggtga acaaattgcc caaatgggta gtttaggaac ttttttatat ggttttttat
     1441 tgcgtttaac aggtgctgtt ggactacacc acaccattta ccctttgttc tggtatacat
     1501 ctttaggtgg taccgaaact gtagctggat caacaattgc tggagcccaa aatattttct
     1561 ttgcccaact agctgatcca aaccacactg gtctgtttac ttatggtact agattttttg
     1621 caggaagatt tgcaacaatg atgtttggat taccagcagc atgttatgca atgtatcgtg
     1681 caattcctaa gaaaaatcgt aaaaagaatg gtgggcttta ttttagcggg gcccttacat
     1741 catttttaac tggaattact gaaccagttg aatatatgtt tttatttgtt gctccttggc
     1801 tttatgtgat tcatgcattc ttagatggat tatcttttta ttttgctgat atcttaaata
     1861 ttcgtgtagg gaattccttt tcaggtggtt tgatcgactt tttattattc ggtgtacttc
     1921 aagggaatga caaaactaat tggttaaaat taattccttt tggtattgca tgggcattta
     1981 tatatttct
//
LOCUS       DACDSY010000252         1983 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.257, whole genome shotgun sequence.
ACCESSION   DACDSY010000252 DACDSY010000000
VERSION     DACDSY010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.257"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="IWK44_002926"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="IWK44_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9551374.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="IWK44_002927"
     CDS             852..1040
                     /locus_tag="IWK44_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9551375.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="IWK44_002928"
     CDS             1053..1610
                     /locus_tag="IWK44_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9551376.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="IWK44_002929"
     CDS             1642..1884
                     /locus_tag="IWK44_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9551377.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          723 a          306 c          406 g          548 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttc
//
LOCUS       DACDSY010000253         1970 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.258, whole genome shotgun sequence.
ACCESSION   DACDSY010000253 DACDSY010000000
VERSION     DACDSY010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.258"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            118..1584
                     /locus_tag="IWK44_002930"
     CDS             118..1584
                     /locus_tag="IWK44_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9551378.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
BASE COUNT          615 a          335 c          400 g          620 t
ORIGIN      
        1 aacgatgtgt attaaataaa agaatacaca cataaaataa cactgtcttg taagtttgtt
       61 aaaaattgaa tatgattaca tctggataac aaatttttat cataaaggag agaacgaatg
      121 aacaacaaac aattacgctg gtttactgtt gcgttaattg catttaacat ggtttggggc
      181 atggggaatg tagttaataa ctatgctcag caaggagtat cagttgtcac ctcttggcta
      241 ttgattttgg ccatttattt tattccatac gcattgatcg ttggacaatt aggatcagcc
      301 ttcaaggata gtaaaggagg cgtcagttca tgggtcgaaa atacgacatc taataaacga
      361 ctggcatact atgccgcttg gacttactgg gtcgtccata ttccttattt ggcacaaaag
      421 cctcaagcaa ttctgattgc tggtggttgg gcagtccaag gaaatggaaa tatcgtaaat
      481 acgatgtcag tacaaatggt agcagttatt tcacttatta tatttcttgc ctttttatat
      541 ttatcaacaa aaggactttc tactttgaaa gtgattggcg gactggccgg aacagcgatg
      601 ttcgtcatgt cgatcttatt tattctttta gctgtaacgg caccgttcat caatccaacg
      661 atggaatttg ccacaccaca tatgaatcaa gtgaaaacat atattccaac atttgatttc
      721 agttatttca caaccgtttc gatgctagta tttgcagttg gaggggcgga aaaaatatcc
      781 ccttatgtca actctacaag aaatcctgca aaggaatttc ctaaagggat gatcttctta
      841 gcagcaatgg tagggatttg tgctgtatta ggctcagtcg caatgggtat gctgtttgct
      901 agtgataata ttcctaaaga cttgatggca aatggggcat acagtgcttt tcaaatttta
      961 ggtaattact atggtatcgg taactcattg atgatcattt acgcattgac aaacatgatt
     1021 ggacaaatct ccgcattagc cttttcaatt gatgcaccac taaaaatctt gttagcagat
     1081 gcggatccag aatttgtacc aaactggcta cgtaaacgta atcgtaaagg tacattggtg
     1141 aatggttatc ttctgacagg tatactagta ggaatcatta ttttgttgcc gatttttggt
     1201 atcaaagata tgaatgaatt agtaaaatgg ttgacgaact tgaactcagt tgtcatgcca
     1261 atgcgttacc tatgggtgtt cttggcatat atgatgttga ataaagctta taagaaattc
     1321 aattctgaat ataaatttgt taaaaatcca aaaatcggtt ttatctttgg tgcttggtgc
     1381 tttgccttca ctgcgtttgc atgtatccta ggaatggtgc caaaagtaga ttatactgct
     1441 gatcctaaag catggtggtt ccaattattt acgaatatca tcacaccaat cgtcttgatc
     1501 ttattaggta tgatcttgcc agcaattgct cgtcgagaca aaaacaaaaa aatcactgaa
     1561 ccagaaacag tggttcagcc ttaaaataga aatacttgtt acaagaggtt gtgacagaag
     1621 tggtcagctt caagaaataa gaaggaattt acgaaaattg cttctcaaat ttttgtgaag
     1681 tttcggcttg tttttcgaag aagctacttc tggaacaccg tgaatcagag gttgtgaaaa
     1741 agctgtcctg catcaagtaa taagaacagc caaacaaaaa tagcccctca tatttttcgt
     1801 ttggctgggc ttattaccgc agatgcaagc tttggaacac cgtttaagga agaggttgta
     1861 aaatcagcga tttgacctga gtattagaga aacaaacgga aaaatagctc ctcatatttt
     1921 gccgcttttt gagctaatgc tgaaggtcag ctgattgaac accatttatt
//
LOCUS       DACDSY010000254         1960 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.259, whole genome shotgun sequence.
ACCESSION   DACDSY010000254 DACDSY010000000
VERSION     DACDSY010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.259"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(74..736)
                     /locus_tag="IWK44_002931"
     CDS             complement(74..736)
                     /locus_tag="IWK44_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9551379.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            1100..1187
                     /locus_tag="IWK44_002932"
                     /pseudo
     CDS             1100..1187
                     /locus_tag="IWK44_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            1245..>1960
                     /gene="erm(A)"
                     /locus_tag="IWK44_002933"
     CDS             1245..>1960
                     /gene="erm(A)"
                     /locus_tag="IWK44_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP9551380.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNS"
BASE COUNT          693 a          348 c          292 g          627 t
ORIGIN      
        1 gttatttata ctaaagcacc cgatagctct gaaaacaatc acaaattcaa cttttcaaag
       61 ccacagcttt aagttatttt gtccagacaa cccccattgc ccaacccatt ttatggaatt
      121 ggcatccagg caacaacttt tcatataaat cgtaataatt ttgttcagat aggtacttat
      181 ctgaagctaa atgctcaagc catgatttag atgtgttgtg attataaatt ctaatcgcat
      241 tttttattcc aaatttgaaa caatgcggta aaaactcttg gattgcccct aatttataaa
      301 cataggtagg cggtgtctcg acttcagata cattatcaag aataactatt cttccttcct
      361 cattcaacag ttccttcatt tgctgtatta cgctggctat atcatccaaa tgatgaaagg
      421 ttgttcggct tacaataaaa tcaaacttct cattaaaatt aagttgttct gcattcatat
      481 tcagatagac cgtatttgtt agttgacgtt tagatttggc aagatcgagc atttgattag
      541 aaatatcaat ccctaccact tcatcataat aacttgctaa tttctccact aacaaacccg
      601 agccacatcc gatatctaat gctctgcctt tctttggaga catattagac acaaagaatg
      661 aataatcatt caaaagctca ttcacgaaat cgtaatcttc tgcaacctta tcaaactgtg
      721 attctattgt attcaaaaag atcccccatt cctactttat cgacattctt tcattactta
      781 ccactttaga tgttttttcg ttggggataa aacttccctt tagacaattt tatccaaaga
      841 caatacaaca gtgcaacttt attaaagtca ctgtccttta tcgcagcctt tactttttag
      901 taaagacagt ggcttctctt atcaagtttc aaaacatatt attttgaaga aaacgtccat
      961 ctgaagtgtc aagtgcaaaa ttacatataa aggtttattc taaaatgaaa agatgataca
     1021 atcatattca gttacataag gaggtttcaa ttatgtgcac cagtatcgca gtagtagaaa
     1081 ttactttatc tcattcataa tgaaaaaaat ggaaaggaga taaaagtatg ggtacttttt
     1141 ctatatttgt tattaataaa gttcgttatc aaccaaatca aaattaattg gttataatga
     1201 acgcttaatg tcagttcatt ataaccagta aggagaaggt tataatgaac cagaaaaacc
     1261 ctaaagacac gcaaaatttt attacttcta aaaagcatgt aaaagaaata ttgaatcaca
     1321 cgaatatcag taaacaagac aacgtaatag aaatcggatc aggaaaagga cattttacca
     1381 aagagctagt caaaatgagt cgatcagtta ctgctataga aattgatgga ggcttatgtc
     1441 aagtgactaa agaagcggta aacccctctg agaatataaa agtgattcaa acggatattc
     1501 taaaattttc cttcccaaaa catataaact ataagatata tggtaatatt ccttataaca
     1561 tcagtacgga tattgtcaaa agaattacct ttgaaagtca ggctaaatat agctatctta
     1621 tcgttgagaa gggatttgcg aaaagattgc aaaatctgca acgagctttg ggtttactat
     1681 taatggtgga gatggatata aaaatgctca aaaaagtacc accactatat tttcatccta
     1741 agccaagtgt agactctgta ttgattgttc ttgaacgaca tcaaccattg atttcaaaga
     1801 aggactacaa aaagtatcga tcttttgttt ataagtgggt aaaccgtgaa tatcgtgttc
     1861 ttttcactaa aaaccaattc cgacaggctt tgaagcatgc aaatgtcact aatattaata
     1921 aactatcgaa ggaacaattt ctttctattt tcaatagtta
//
LOCUS       DACDSY010000255         1880 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.260, whole genome shotgun sequence.
ACCESSION   DACDSY010000255 DACDSY010000000
VERSION     DACDSY010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.260"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IWK44_002934"
     CDS             5..1150
                     /locus_tag="IWK44_002934"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP9551381.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IWK44_002935"
     CDS             1321..1779
                     /locus_tag="IWK44_002935"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP9551382.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
BASE COUNT          617 a          355 c          373 g          535 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat
//
LOCUS       DACDSY010000256         1877 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.261, whole genome shotgun sequence.
ACCESSION   DACDSY010000256 DACDSY010000000
VERSION     DACDSY010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.261"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..1072
                     /gene="xerS"
                     /locus_tag="IWK44_002936"
     CDS             2..1072
                     /gene="xerS"
                     /locus_tag="IWK44_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP9551383.1"
                     /translation="MYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKNYKLFY
                     EWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNNNLGLS
                     TSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARANAIEKK
                     LFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSEVININ
                     LDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKALFLAIY
                     QGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQLGHSS
                     TNTTTLYTHIDNAATIDALNAL"
     gene            complement(1356..1727)
                     /locus_tag="IWK44_002937"
     CDS             complement(1356..1727)
                     /locus_tag="IWK44_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9551384.1"
                     /translation="MTVMRLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPE
                     TASSIRTIELDDNTITFLKAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIV
                     GVHIIRIHGLRHSHASLLISQ"
BASE COUNT          667 a          320 c          293 g          597 t
ORIGIN      
        1 aatatatcaa aaaatagata caatcatact tcatatgccc gattatataa aagattttgt
       61 tcaagatcgt gaggataaag atcaatcacc tcgaaccact ttagaatatc taaaaaatta
      121 taagctcttt tatgaatggc ttctgagtga gtctatcgtt cctgatactt ctatcacctc
      181 gataagacag ttaactgcct atgacctaaa cttgtacaaa tctcatttga aacgtcgcac
      241 aaaagaaaat gtaaaaaaag agacaacaaa aactaagctc aaagataata ataacttagg
      301 tctatctact tctactatca atagaaatat cacggcatta aaggttctat ttaaatatct
      361 ttcgaaaagt tccaataatc ctctaggaaa accatactta gacaataatc ctatggatca
      421 agtagcaacc atcacagata aaactacgtt agctgcacgt gccaatgcca ttgagaaaaa
      481 attatttcta gataaggata cacaaaatta tctagattat attgccaatg aatataaaaa
      541 tactttgagt aaacgagctt tgatctatta tcaacgagat gtagaaagag acctggcgat
      601 caatgcactg attttaggat ctggattgag actctccgag gttataaata ttaatttaga
      661 tgacctatct ttagataaga ataatgtggt agtaactaga aaaggaaata aaagagatgc
      721 tgtcaatata gcagcattcg caatggaata tttagctaat tatctagcta ttcggaaaga
      781 acgatataaa gtaacagaaa acgaaaaagc tttatttcta gcaatttacc aaggagaagc
      841 caagcgtata tccggtattg caatcgaacg gatggtagca aaatattcaa aaggctttag
      901 aattcaagtc agccctcaca aactgcgtca tacattagct actagactct accaacagac
      961 aaattcattg gtattaactg cccaacaatt gggacattca agtaccaata caacaacttt
     1021 atatacccat atagacaatg cggctacgat cgatgcatta aatgctttat gagtataatg
     1081 tataaatagg cggaaataag aaacaacacg ctagaaaagc ctgtattata gtttttctag
     1141 tgtgtttctt ttggtgtgat cctacttttg tttatcattt attttttatt atcgtaaata
     1201 ggtgtcggtt tgattttaga aaaattagtt tggatataat ctgtgggatc atttgttatc
     1261 tctctcaatg aatttagata caaatgacca taagttccta aagtcgtttg tacatcagca
     1321 tgttttaacc tttctttaat tcgaagtgca tctttttatt gtgatataag caaagaagca
     1381 tgactatgcc ttaatccgtg tatgcggatt atatgaacac caacgatcgt tgcatgtctt
     1441 ctcataatat gacggattgt atatttctga gtaggtgttc cattataaga aagtacaaac
     1501 gacatgcctc ctattgaaac ttgacgctcc ttccaagctt ttaaaaaggt aattgtattg
     1561 tcgtctagtt cgattgtacg aatactagaa gccgtttcgg gttctacaaa ctcataggtt
     1621 ttggctctct tataatagag tgttttattc acacgcaagt ttttattttc aaaatcaata
     1681 tctttccaag ttaatgcagt ggcttctccc aaacgcataa cggtcataaa aagtaaccac
     1741 atacaaataa aaccaaaatg ttggtacaaa tcatcaatat aaaaagttga aataactttt
     1801 gtaaattctt cttttgtcca aaaatcaact gtagttttcc ttttcttcac atttccaata
     1861 acttttgcag gattttt
//
LOCUS       DACDSY010000257         1833 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDSY010000257 DACDSY010000000
VERSION     DACDSY010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.262"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..969
                     /locus_tag="IWK44_002938"
     CDS             <1..969
                     /locus_tag="IWK44_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9551385.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="IWK44_002939"
     tRNA            1159..1232
                     /locus_tag="IWK44_002939"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          605 a          317 c          346 g          565 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg
     1321 agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact
     1381 tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg ggttaaattg
     1441 tgagataata ataaagaaga gtttaaagcg caccccaaac cacttcccca taagtgtgtt
     1501 acgctttaaa ctcttttata tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa
     1561 gtgagaaaaa aggcttacaa ttggagtggt agttaattag tgacttattt ttgattttat
     1621 agcactgata ctataaaata tagatatcat catattacac aatcttaata ctaacttaaa
     1681 aaatatctcc tttcacaagt atggtgataa aattcgttcc gggctacctt tttaggtagc
     1741 ctactttaat ctttatacct ttctggatca acgaaagtat actttatata gtcataacgc
     1801 cgatgatcgc tacgtgcgtc cggcacgtca gtc
//
LOCUS       DACDSY010000258         1813 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.263, whole genome shotgun sequence.
ACCESSION   DACDSY010000258 DACDSY010000000
VERSION     DACDSY010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.263"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..537
                     /locus_tag="IWK44_002940"
     CDS             <1..537
                     /locus_tag="IWK44_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9551386.1"
                     /translation="NKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKK
                     LNMPSEQYGIAVHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDC
                     RINNYVHLSPGVCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHS
                     LAYGNPAKIKKEGINFEF"
     gene            527..1669
                     /locus_tag="IWK44_002941"
     CDS             527..1669
                     /locus_tag="IWK44_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP9551387.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1682..>1813
                     /locus_tag="IWK44_002942"
     CDS             1682..>1813
                     /locus_tag="IWK44_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9551388.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     "
BASE COUNT          642 a          240 c          385 g          546 t
ORIGIN      
        1 aataaaatgc aagatctgaa atatcttggg aaaataaacg aattgaatag ctgggcggat
       61 agcaaaaaaa aaatctataa atttattgta gctattggtg actgtgtagt aagaaaaaag
      121 attataaaaa aattgaatat gccaagtgag caatatggaa tagctgttca tccaagtgct
      181 atagtggcaa aagaaatttc aattggttat ggaactgtta tttttgcgaa tgctgtaatt
      241 aacccagatg ctgtaattgg agagcatgct ataataaaca caggtagcat tgttgagcat
      301 gattgtagaa ttaataacta tgttcattta tctccaggtg tatgcttagc gggtggagta
      361 catgtaggtg taggaactca aataggaatt ggttctcaat gtatccagat gaagaaaatt
      421 ggatcttggt gtatgatagg cgcagggagc actattgtta aggatattcc ctcacattcc
      481 ttagcttatg gcaatccagc taaaattaag aaagaaggaa ttaattttga attctagaat
      541 tttattatca agtcctcata tgggaggaac agaagaaaaa tatgttgcta aggcttttaa
      601 agataattgg atagcgccat taggtgaaaa tgtggataaa tttgaagaaa gtattacaaa
      661 atatactggt tctaagggcg gagcttctgc gttaagttca ggtacagctg ccatacatct
      721 tgccttaaga ctgcttaatg ttggaaaaaa cgatattgtc tttgctcctt catttacttt
      781 tatagctaca gtaaatccta ttttatatca aggggctgta ccagtgttca ttgactcaga
      841 acctaataca tggaatatga gccctgttgc actagagagc gcattagaag aagctgaaag
      901 aaacaaccaa ttacctaagg caatcatcgt agtgaattta tatggtcaaa tggctgatta
      961 tgaacgaatt ttagctattg caaaccaatt taatgttcca gtgatagaag atgcagcgga
     1021 atcgttaggt tctacttata agggaatttc gtcaggctta tttgggacaa ttgggatcta
     1081 ttcatttaac ggtaataaga tcatcacaac ctcaggtgga ggagctctag tgtctagtga
     1141 taaaaaatat gttgagcatt caagattttt agcaacacaa gcaagagatc aagcaccgta
     1201 ttatcaacat agcgaaatag gttataatta ccgtctaagc aatatttctg ccggtatcgg
     1261 acgtggacaa atggaagtat tgaatgatag agtagctaaa cgaagagaaa ttttcaatcg
     1321 atataaggaa gctttttctg gaattgatag aattgaaatg atgccagaag tggatggcat
     1381 ggtgccaaat cgttggttgt ctactttaac gatcaatgat tataattcta ttgatcaagt
     1441 agttaacgta ataaataaac taaatgaaaa aaacattgaa acaagagctt tgtggaaacc
     1501 actacatttg caaccagtat ttcaaggggt aaaaatgtat taccatgata ataatcttag
     1561 tgtgtcagaa aagttattta atagcggctt atgcttacct tctggaagta atatgacaga
     1621 tagtgaacaa gaatttgtta ttgatgagtt taaaaaaata ttcaagtagg tgtgatattt
     1681 tatgaacata ggaattattg gtttaggttc catggggaaa agaagactta gactcattca
     1741 agaacacttt aatgatattt tactatgtgg tgttgatact aatgaggcga gacgaaagga
     1801 agttgaaaag gaa
//
LOCUS       DACDSY010000259         1810 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.264, whole genome shotgun sequence.
ACCESSION   DACDSY010000259 DACDSY010000000
VERSION     DACDSY010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.264"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..413)
                     /locus_tag="IWK44_002943"
     CDS             complement(<1..413)
                     /locus_tag="IWK44_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9551389.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLF"
     gene            complement(547..1737)
                     /locus_tag="IWK44_002944"
     CDS             complement(547..1737)
                     /locus_tag="IWK44_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP9551390.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
BASE COUNT          516 a          369 c          332 g          593 t
ORIGIN      
        1 tcaaataatt gctttctaat agctttgtta ggtctaataa atagagaaat actacctaca
       61 cctttatctg catttgaaat caattcttct aagattgttt gactttcaga atttaaagaa
      121 atggaagcac aaatctcatt gtcaaattct tcttcgctga ttccttcaaa tgtcgcagag
      181 ataataatcc aatgcccttg ccacccattt tgaattccat gaaaaaaatc tgattctttt
      241 agtgttttac tactatagta ataactatca tctaataaga ttctcaatgc agtaagagca
      301 ttggttttcc ctgaatcatt ctcacctata atagtatttg tctcattatc gaatcgaaag
      361 atttcggatt cataatttct aaaatttcga atttgaagag tagatataaa cataagcaga
      421 tctcctaact atttaattaa tatgattata tagtaagttt atcataaatt atgttagtaa
      481 aagttctcat tataagggag cgtcaataat tttgtgtaaa tgattcttcc ctactgcaga
      541 ttgtttctaa ttctcaatca gttggcttag catttgttcg agctcgcctt cggcctgttg
      601 gaaaccttta tgactgcgac ctaggaattt ttggttgtaa gtatcaaaag acgagactaa
      661 gaaacgttcc atcgactctt cattttggaa ctgttccttg cggtggctgt atttcttgat
      721 ttgtttatta aaggattcaa tcaagttcgt agaataaata ctcttgcgta tggccaaggg
      781 gaaatcatag aaagtgagca agtgatcgtt cgaaagaatc gattcaacca cttttggata
      841 gctggttttc catttttccg caaaagtacc acgtgcttcc aacgccacct ctttagatga
      901 ggcttgatac accattttaa aatcatcaca gacttcctta cgatcatcga cacgcacttt
      961 atgactgata ttacgggaaa cgtgtacaca acaatgttga aaacgagctt tgggatagaa
     1021 ccgactaatc gctcctacca tcccctttaa gccatccgtg atgaacagga ggacattttt
     1081 caaaccgcgc tcttgaaggt ccaataaaat ttcctcccaa atcgtgatgg attcagtcgg
     1141 tgcaatcgca tagctcaata cttcctttga tccgtccggg cgaatgccaa ctgcgatatg
     1201 aatagcttcc ttggcgacgg ttttccgctt taacggaata tacgttgcgt ccatataaat
     1261 agcagcataa cggtcatgaa gctcccgccc attaaacgcc gttacctctt ctgtaaatga
     1321 ttttgttata ttggacatgg tttggggcgt gtagtaatgc ccatacattt tctcaatcaa
     1381 gtctgcgatt tccgacatgg taataccttt tcggaagagg tgaatgacgg tctcctctaa
     1441 cgtgtcattc gtccgtctat aagcaggaac agtctgttgc ttgaactcac cattgcggtc
     1501 gcgcggaatc tggagatgaa gttccccata ctcggtcttg accgtacggt cataggagcc
     1561 gttacgagaa ttacccgtgt taaaaccaat gcgatcatac ttttcgtaat ctaggaaagc
     1621 cgttaactcc gttttgagaa gcgtattgac tgctttctcc aaatggacac ggaataattc
     1681 attcaaatcg cctttgttcg ctagagtctt tagaatttct gtagtaaaat cgttcatagg
     1741 gaagtcctct cttctgtgaa tgtgttgtgg taactctatt ctacagaagg gacttccttt
     1801 tttctattta
//
LOCUS       DACDSY010000260         1799 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.265, whole genome shotgun sequence.
ACCESSION   DACDSY010000260 DACDSY010000000
VERSION     DACDSY010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.265"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="IWK44_002945"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="IWK44_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9551391.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          506 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaa
//
LOCUS       DACDSY010000261         1792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.266, whole genome shotgun sequence.
ACCESSION   DACDSY010000261 DACDSY010000000
VERSION     DACDSY010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.266"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1619
                     /locus_tag="IWK44_002946"
     CDS             <1..1619
                     /locus_tag="IWK44_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9551392.1"
                     /translation="NPEEDDEALLGSRIDMSDIFKHISVVYVDALRDVLREMKNNRNP
                     VKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIGENINRQLNSIIGSVYSP
                     NLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIYLALKIVEFEACRSRELL
                     NIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVHLAEVSEISRMNVLKSFN
                     GKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNGLLFSKGVILVEGDAEEI
                     LIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRIQRKCAIVTDLDEQAIPS
                     EHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEIEFLDVNKENILTYVSPI
                     IDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGWFAIELGEKIDFLIKIPK
                     YILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSASLKTSDTADLNIAIISL
                     LEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            1616..>1792
                     /locus_tag="IWK44_002947"
     CDS             1616..>1792
                     /locus_tag="IWK44_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HAP9551393.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRII"
BASE COUNT          687 a          241 c          308 g          556 t
ORIGIN      
        1 gtaatcctga agaagacgat gaagctctgt tgggctcaag aattgacatg tcagatattt
       61 tcaagcatat ttcggttgtt tatgttgacg ctcttcgaga tgttcttaga gaaatgaaaa
      121 acaatcgaaa cccagtcaaa agaatcatgg aaacaattga atcaaaaata tcatcagata
      181 atgttgagtc attaaaaaca attatacagc aattaaatga gactattacc agtgttcctg
      241 aaattaggaa aattggcgaa aatattaatc ggcaactaaa tagcataatt ggttcagtgt
      301 attctcctaa tcttcttctt tcctcaacta tgtcagatga tatgggttct ttagcaaaat
      361 ttctttcaat gaagccagaa caaaatatcg acttagactt actagggtta ggacatctaa
      421 atatgattta tttagctttg aaaatagtcg aatttgaagc gtgtcgttca agagaactac
      481 ttaatattat gttgattgaa gaaccagaag ctcatattca tcatcatatt caaaaaactc
      541 tgttcgaagg attaaatctt caaaaaaact atactcagat tctcatgacc acacattctg
      601 tacatttagc tgaagtgtct gaaatatctc ggatgaatgt attaaaatcg tttaatggca
      661 aatctatcgt tatgaatcca gcaaataatc tgaactcatt tgctaaagaa aagttaaaaa
      721 agaacaatct aaatctaata aacgcagtag agagatattt agatgttaaa agaaatggat
      781 tgcttttttc taaaggtgtt atcttagttg aaggagatgc tgaagaaata cttattcctc
      841 aaatagtaaa aaaattattt ggtataaact tagacgaact tggaataggt ttgattaatg
      901 ttggtagtac ttcgtttgaa tatatcgcta gcttgtttga cgatgataga atacagagaa
      961 agtgtgctat tgtaactgat ttagatgaac aggcaatccc atcagaacat cgattgtata
     1021 aaagcactgc acaagagaaa ggtcaagcaa gaaaagaaaa attatcctcc ctttttaatg
     1081 aaaacaattg ggttaattgt ttttatgcta atacaacatt tgaaattgag tttttagatg
     1141 tgaataaaga aaatattcta acttatgtca gtccaattat agatgacgca ttcaaagata
     1201 aaaaaacaat aactgcttat aaagaagaaa tacaaagtga acaatattta gaaagaaatt
     1261 tagcgatgtt aaaactagcc aatcatgttg gtaaggggtg gtttgcaatt gagttaggtg
     1321 aaaaaattga ctttttaatc aaaattccta agtatattct taatgctatt gcttttgcat
     1381 gtcaggaaat agtagatctc tcaatttata aaaaaattct actccataca atcgacttgt
     1441 acgaggaaac tacagaattg tcagctttat cagctagttt aaaaacaagt gatacagctg
     1501 atttgaatat agcaataatt tctttattag agtactttgg taaagatgag gatgttgtgt
     1561 atctaatcaa tcagatagaa aattacacaa aaatacttta tgctgaggtt tcaaaatgac
     1621 aaatatcttc gaaaaaatcg taaatgattt aaatttagaa caaaaagatg ttgtggaaag
     1681 tgaagataac ctttatgtaa atgcatgtcc tgggagtggg aaaacaaggg tacttacaag
     1741 gaaaattgct taccaagcaa ttaaacacga tggatcacta aaaagaataa tt
//
LOCUS       DACDSY010000262         1783 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.267, whole genome shotgun sequence.
ACCESSION   DACDSY010000262 DACDSY010000000
VERSION     DACDSY010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.267"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..774)
                     /locus_tag="IWK44_002948"
     CDS             complement(<1..774)
                     /locus_tag="IWK44_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9551394.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNFHLDSTHIKANA
                     NKHNFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEQEKQFAYSAHTSCDDNGFILSTIITPGNIHDS"
     gene            952..>1783
                     /locus_tag="IWK44_002949"
     CDS             952..>1783
                     /locus_tag="IWK44_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9551395.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     V"
BASE COUNT          572 a          327 c          333 g          551 t
ORIGIN      
        1 actgtcatga atatttccag gagtgatgat tgtgctaaga ataaatccat tgtcgtcgca
       61 tgaagtatgg gcagaataag caaattgttt ttcttgttcg cctttgacgt agtagccgct
      121 ttccggatca gctttactta tttttctttc tttcagcttc acttcctttt ctaagtccaa
      181 agtaaaaggg cgttttccag cctctattcg ttgacgattg atctcatctt ctaattcatc
      241 ctgataggct ttcgcttcat catgggtcat ttctttcgta aaattatgtt tattggcgtt
      301 agctttgatg tgggtagaat ctagatgaaa attatcctct gttacaaaac ctgctttaac
      361 tgcttgttct aaaatatagg caaaaatatc ctcaaacacc gtcgtttctc taaatcggcg
      421 aacataattc tttccaaaag ttgagaaatg ggggattttc tcttcgaagg agtagcctaa
      481 aaaccagcgg taagcgacat tggtatcgac ttccttaatg gtttgtcgca tggaacggat
      541 accgaataaa tactgaataa acaccaattt gatcagaatc actgggtcga tacttgggcg
      601 acctaaatcg gagtaagtgg cttcgacgat tggataaata aattcaaaat caagcgcggc
      661 atcaatcttt cgaacgagat gatcttgagg aactagatca tcaatcgtaa gcatcgcaaa
      721 ttgatttcta ccattcgtat tgtttttaga catcatagct atcactcctt gcattcgttt
      781 tcataaatct attataaaag aaaagactgt agacatcatc cataaaatgg actttgtcta
      841 cagtctggca ttttattacg ccgattgtaa aattaagcta gacaactaaa aaagtcattt
      901 gtgctacact caaaatagtt ccgaccaaag aattataagg agtgagtaca aatgacctat
      961 acccatctta caacggatga acttgtaata atagagtctt atttcaaaat gaatcaatct
     1021 gttgctaaaa ctgcccattg cttgaatcgt tcaagacaaa cgatccataa agtatatctg
     1081 ttcttcaagc aaggaaaatc agccttagaa tattatcaac agtataagaa aaacaaatca
     1141 aactgtggta gacgtccgct tgttttaccc gaggaacaat cagaatatat tcaaagaaag
     1201 gttgttcaag gatggacacc cgatgtgatt gttggtcgtg cagcgtttcc tattagttgt
     1261 tctgctcgta ccatttatcg tatgttcaaa aaggggctat ttgattcttc tgacttaccg
     1321 atgaaaggca agcgtaaacc gaatggacat caagaaagac gtggaaaaca aactttccgc
     1381 cgctctattc atgaacgtga aaaggattat agccaattct caaatgagtt tggtcacctt
     1441 gaaggtgaca ctatcgtagg tctgaaacat aaaagtgctg taattacctt agttgaacga
     1501 ttatcaaaag ttatcatcac attgaaaccg tgtggtagac aagcgattga tattgaaaaa
     1561 aaattaaatc aatggtttga atctgtaccg aaaaacctat tcaaatccat cacttttgat
     1621 tgtggaaagg aattttcaaa ttggaaacag atcagtaatg tcaatgatat tgccatttat
     1681 ttcgctgatc caggaacgcc gtctcaaaga ggcctaaacg agaattctaa cggattgtta
     1741 cgtagagatg gtttattgaa atctatggat ttcaattcag tag
//
LOCUS       DACDSY010000263         1738 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.268, whole genome shotgun sequence.
ACCESSION   DACDSY010000263 DACDSY010000000
VERSION     DACDSY010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.268"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            40..1675
                     /locus_tag="IWK44_002950"
                     /pseudo
     CDS             40..1675
                     /locus_tag="IWK44_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          559 a          276 c          306 g          597 t
ORIGIN      
        1 tagataaagc aatccatgaa aggagtatat aattaagtaa tgcaagtaaa gaaaaaaata
       61 atattgtcca ttctcctagc aattattgct ggactttatt caatcaatta ttttagaaat
      121 gttcatacta tttctacatc gggggatatt gccttccatt ttgctagagt caaagggtta
      181 tcttcaattt tttctggtcc aatcaatttt acgacgttta atcattatgg gagcggagtg
      241 aattattttt atccctactt gacctttttt ccggcagtca ttttttattg gataagtaat
      301 aatttaatcg ttagttatat cttatatgtt tggctattga atgtttgtac tatcatgctg
      361 atgtttcatt atggactaaa atttttaaaa agaatcgatg cggcttttat atttagctgt
      421 ttatatacat tttatgggta ccgtacgatt gatatttatc atcgttctgc tattgctgaa
      481 gcgattgcac ttacggttat accgatagtt atgtattatg cttacgcact gatttatgag
      541 aaaaagccat gtgcaattcc attagcagtc agtatgagct tactcattta tacacatgtt
      601 ctttccactt ttatgagtgt aatagccgtt ggcttactag tcttaggcgg ctttgtagta
      661 agtgaaacaa gaaaaaaaga aagcatccgg ttgattgtgg agttgtcaaa ggcagtagga
      721 atgacgattc ttttaacagc atacttttgg tatccgatgt tccgacaaat catggttcaa
      781 tcgattaatg agccagataa aacgacctta caacaccaag cattaaatgt ctcggatagt
      841 ttgattggtg cgatgaataa tgacctgaca acctttacgg taggaatcat tggtattatt
      901 tctttgatct gtccactctt cttttttaaa ggatttaatc gcaaagaaaa agtgatttac
      961 cttagtgcgg tagtctcttg gctcttatcc accaaatttt ttccatggtc tttattacaa
     1021 aatacaccca ttcaaatgat tcaatttcct tggcgaatct taggctttca agtactgttt
     1081 ggctcgttga ttttatcgtt cgttttcatg aagagcacat cagctaagca aagaaaagta
     1141 gttaatataa cggcaattgt tttattactg ttggctgtat ctgctgcaac aaaagcaaac
     1201 tatgaacata aagttgtcac tcagagagat cacttggtaa tgaatcaaga aaatcttatt
     1261 cgttacacga cactccttcc cggaggactt tatgactatg cacccacgga agcattacaa
     1321 tacaaagaac atttgcagaa tcatgaagta agaattggca aggaatggca tactatgcct
     1381 tatcaagtga tggattcgaa aatagtctat acagtacatg aatctaaagg acaaaaaata
     1441 acgcttcctg tatttgatta ttggggaaca gtcgcaaaag taaatggaca aaaaagagag
     1501 attgataata ggactagtgt cgattgaagg aacacaagga gaaacaatca ttgaagtttc
     1561 ttccatttat cctccaacta tgaagttagc ttttttaatt agtttatgta gttacctctt
     1621 tcttttaatt agttttctta tcaggaagtt acaacatata aaaataatga tctgattaca
     1681 tttttttcaa tattagctta ctagagaata agaaaaagta agtaataaac caaattta
//
LOCUS       DACDSY010000264         1721 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.269, whole genome shotgun sequence.
ACCESSION   DACDSY010000264 DACDSY010000000
VERSION     DACDSY010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.269"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            83..895
                     /locus_tag="IWK44_002951"
     CDS             83..895
                     /locus_tag="IWK44_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP9551396.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            892..1692
                     /locus_tag="IWK44_002952"
     CDS             892..1692
                     /locus_tag="IWK44_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP9551397.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
BASE COUNT          524 a          272 c          392 g          533 t
ORIGIN      
        1 caatgatttc ataggaaaaa cagttagata ttgctataat acttaagata gtgggaatct
       61 tttaagtata ggaggaaaaa gaatgttacg tttttttccg caaaaaaaca aatatattca
      121 agaaaaaagc gaatttcaat ttaataccga tggaccaatt cctcagcaca ttgccatcat
      181 catggacggc aatggtcgtt gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa
      241 agaaggcatg gaaacagtaa agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt
      301 gttgacgctt tatgcctttt cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt
      361 tctcatgcaa ctacctgtcg acttctttga tacgttcgta ccagaattga ttaaagaaaa
      421 tgtcaaagtt catgtcatgg gttatgaaaa cgtattgcca gaacatacgc aagatgctgt
      481 aagacgtgca attgagcaaa caaaaaacaa tactggaatg gtattgaatt ttgctttgaa
      541 ttatggaagc agagcagaga tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa
      601 aggagaaata cacgcagaag aaatcgatga tgaattgatt gctaagcatt taatgacagg
      661 tttcttgcct aaagagttac aagatcctga gctgatgatc cgtacaagtg gagaagagcg
      721 aattagtaat tttctgttat ggcagatcgc atatagtgag ctgtatttta caaaagcgct
      781 ttggcctgat tttgatggtg cgcatcttga agaagcaatc gcctcttatc aaaatcgaga
      841 ccgtcgattc ggcggtgtaa aaaaaacaga agatgagaaa ggggatcagt catgaaacaa
      901 cgtgtggtca cagcagccgt ggcgctagtc ttgtttattc cgattatatg gatcggaggc
      961 atagcggttg agcttacagc agcaatctta gcagtaatag gagtttacga gctgtttcgg
     1021 atgaaaggat tgacactctt aagttttgaa ggagttttat ccgccgttgg tgcagtgtta
     1081 cttgttttgc caaaagaaag atggtttttc tttcttccag agaatgtaag tacatttatg
     1141 ttattttatc ttatagttat gattctttta ggaacatcag ttatttctaa aaatacgtat
     1201 acgattgatg aagcaggttt tcctgtgctt gtcagcttgt atgtgggaat aggtttccag
     1261 aattttgtca atgcacgaac agaaagcttg gctgttttga tatttggtct atttattgtt
     1321 tgggcaactg atatcggagc atatatggtt ggtcgacgtt atggtcagca taagttaagt
     1381 cctgatattt cgcccaacaa aacgattgaa ggtgcgctcg gtggtattgg tagtgctgtt
     1441 gtagtcgcat ttctttattt attgctatac ccagcaaaag aactatttaa tcatggtatg
     1501 tttgtcatgc ttctgatgac gattattttt tcagcagtcg gacaatttgg tgatttggtc
     1561 gaatctgcaa tcaaacgtca ttatgatgtg aaagactcgg gaaatatctt accaggccat
     1621 ggtggaatct tagaccgttt cgacagtttg ctgtttgtat ttcctatcat gcatttattt
     1681 ggcattattt agtaagtata cctgcgtttc tttgtgaatt t
//
LOCUS       DACDSY010000265         1714 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.270, whole genome shotgun sequence.
ACCESSION   DACDSY010000265 DACDSY010000000
VERSION     DACDSY010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.270"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(434..724)
                     /locus_tag="IWK44_002953"
     CDS             complement(434..724)
                     /locus_tag="IWK44_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551398.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(932..1624)
                     /locus_tag="IWK44_002954"
     CDS             complement(932..1624)
                     /locus_tag="IWK44_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9551399.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          440 a          338 c          271 g          665 t
ORIGIN      
        1 gtctcaaaaa tccaaatgtt aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc
       61 gaggcttctt caatttactt ttacgactat gataatcacc tcttcgagtt acataccggt
      121 actctccagg aacgtttaaa atcctacaat tccactacct gagactagta atgactagtc
      181 tctttttatg gcctctgatc cccccattga gcgaatacta atagggcttc gaattgatct
      241 gttcaatctt taagcaatat ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa
      301 tacttaaata catgtagtta tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg
      361 ttcaaatccc atcgtctgca tccatctttc caaaacaagc ggctaggaca tttttgtctc
      421 agccgcttgt tttctagttt attctttcat cttgtcggac cttttttctt gggtatagac
      481 ttttctcata ttctaccaca attttgggtt tccctaacac atgataggat ctcacaacaa
      541 attgctttct attattataa taacctgtaa cgactaactt catcccttcg tctacttcag
      601 ctaggaagtt caaactatgg gaagcaatca aacaattgac cccatttaaa ctgaaacgga
      661 tcaacggtct ggcactaact tgcaaagtac ggatcttatc tactgttcca tattttttct
      721 ccataacctt cacctcaata ccattatacg aacatacatt ccctttgtaa agaagaaact
      781 ctgttttatc tttttgactt tttgtctatt acctttctta aaatcatatt ggcacttcct
      841 gcgaagatga aggacatgta ctaaaaaaaa gaggcagcag tatagattac tgttgcttct
      901 ttactaattt aggtttactt gttaagaaag attatttacg caaatgttct tcaacaattt
      961 gctgagattt atcagctgta tcttccactt catcttttgc tttttctaac ttttgaatat
     1021 attgtttcaa gcttgagttt tcagcagttg cttcgtctaa tttcttttgt gtatctgtta
     1081 actgagtttc tacatttgtt ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat
     1141 ttacagtatt ttgtaattct gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat
     1201 ctgcattcag atgattgatt tcatcatttt tttggttgat ctcatctagt ttgacattga
     1261 tttcattttg tttgctttgt agttgtgcat ccttgctgac taattgactt tctaagtttt
     1321 gtttttctgc tgccgattga tttttttggt tttctaattc atttactttt gtttggtatt
     1381 cttggacatc tgcctttgaa tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa
     1441 gatctgtaag cgcttgctct ttatctgtca atgtatgatc aagctgtgtc agattctcat
     1501 tgattgtatc aatatctgtc tctccacccc acaaaataat ttcatttgcg taccgcgtgc
     1561 ctgtgtagcc aattagacat agaaatgcaa cgacaatccc accagtaata aatttctttt
     1621 tcatttgcat agctccttaa tccaatactt ttacatgcta aaagtaagtg atctcaaaca
     1681 caatgtcaag cgtttttaaa ttacatgtgt aatt
//
LOCUS       DACDSY010000266         1701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.271, whole genome shotgun sequence.
ACCESSION   DACDSY010000266 DACDSY010000000
VERSION     DACDSY010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.271"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1097)
                     /locus_tag="IWK44_002955"
     CDS             complement(<1..1097)
                     /locus_tag="IWK44_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9551400.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..>1701)
                     /locus_tag="IWK44_002956"
     CDS             complement(1227..>1701)
                     /locus_tag="IWK44_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9551401.1"
                     /translation="KLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLLSLGISKLEIFG
                     QFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAE
                     AEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
BASE COUNT          539 a          340 c          275 g          547 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt a
//
LOCUS       DACDSY010000267         1693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.272, whole genome shotgun sequence.
ACCESSION   DACDSY010000267 DACDSY010000000
VERSION     DACDSY010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.272"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            450..1580
                     /locus_tag="IWK44_002957"
     CDS             450..1580
                     /locus_tag="IWK44_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9551402.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          486 a          275 c          353 g          579 t
ORIGIN      
        1 acgggattct ttcaacttat ttgttatttt tttctaaaaa atattaaaga ttgttaaatt
       61 tcactatttc atttcaaatt ccattgccta atcattagga aaagtctatc atgaactcgt
      121 aaatagaata tagtaataac cttgttaggt gaggctccta tacaaacata ggctgttgcc
      181 cagaaacgtc gaaagtcgcc aatgggtaaa acagaaactg ttggattaag gctttttcca
      241 atagctaaga atgttttctt tacgttgtat agtgctaaaa ctcggacgat gagatgatgc
      301 taggtcagaa ttcttgttcg aaaaggtttc tgaccttttc tttatatttt tgtgactgac
      361 ttatttgatg agacaaactc gctcctcacc tgacaagtgg attgctgtgt gaggaggaga
      421 tgcggtgaaa aaagatcgat tggtggattt tgggtcctta tttgactttg tccatgatcg
      481 gtcttttaga agtatacagt gccagttctt atcgtttatt gcaagccgat gaaaatacaa
      541 aaagtctatt attgcgtcaa ctaattttca tatttttgag ttggagcgtg atcttcttag
      601 ctcgttcagt caaactacac tatttacttc accctaaaat agcaggatac ggtttagcct
      661 tatcgatttt ctttttagta ttagtaagaa tagggatatt cggtgtcact gtcaacggcg
      721 cacaacgttg gatctctctg tttggcattc aattccagcc ttctgaactg gcaaatcttt
      781 ttttgatttt ttatttaagc tggttttttc gtgacggaaa tagtagccca aaagatctaa
      841 aaaaaccatt cctgattaca gtaggtataa cttttctgat tttatttcag ccaaagattg
      901 ctggagcatt gatgatcctt tcgattgcgt gggtcatatt ttgggcagcg gcggttccat
      961 ttaaaaaagg gatctatcta atcgttactt tttctgcatt gctgattgga gcagcaggcg
     1021 gggtattata tttaggaaat aaaggttggc ttccacagat gtttaatcat gcttatgaaa
     1081 ggatcgcgac tttaagagat tcttttatcg atagtcatgg tgcgggctat caaatgaccc
     1141 attcgtttta tgcactatat aatggtggca tatttgggcg gggattagga aatagtatca
     1201 caaaaaaagg gtatcttcca gagacagaaa cagattttat tttttcgatt attacagaag
     1261 agcttgggct aatcggtgca ttgtgtgtgc ttttcttatt gttctcatta tgcatgagaa
     1321 tcttttgttt aagcagtcgc tgtaaaaatc agcaagctgg attgtttctt ttaggattcg
     1381 gaactttgct attcgttcag acgataatga acgtgggaag tatcgcgggg ctgatgccaa
     1441 tgactggtgt gccattgcca tttgttagct acggaggaac aagttatctg atcctttctt
     1501 taggaatcgg aatcacgttg aatatttctt caaagataca ggccgaagag ttggcgctct
     1561 atcgacctga aaaacaataa agagaaactg agaaactctc agtttgaatt atcataaagt
     1621 acaatctttt atttttcaag cgagaggttc ttttagaaag aggaattcgc aatgaaagta
     1681 tctcaaaaaa tca
//
LOCUS       DACDSY010000268         1680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.273, whole genome shotgun sequence.
ACCESSION   DACDSY010000268 DACDSY010000000
VERSION     DACDSY010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.273"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..1452
                     /locus_tag="IWK44_002958"
     CDS             1..1452
                     /locus_tag="IWK44_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9551403.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
BASE COUNT          624 a          280 c          315 g          461 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
//
LOCUS       DACDSY010000269         1669 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.274, whole genome shotgun sequence.
ACCESSION   DACDSY010000269 DACDSY010000000
VERSION     DACDSY010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.274"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..400
                     /locus_tag="IWK44_002959"
     CDS             2..400
                     /locus_tag="IWK44_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9551404.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="IWK44_002960"
     CDS             384..1079
                     /locus_tag="IWK44_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP9551405.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          624 a          244 c          330 g          471 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattactt atttctcatt taaacgatgt
     1201 attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt gaataaattt
     1261 cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct agtataacag
     1321 aataagagga acaagtccag aagggatatt tttagtcttt tttaaaataa cttttcagtc
     1381 gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa atagggaagc
     1441 atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa acttttttaa
     1501 attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat aaaaaaaggc
     1561 cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt tccaaaaatc
     1621 acttctcaaa ttttgccaca tccccaattg ggtcaaattt gtttatttg
//
LOCUS       DACDSY010000270         1664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.275, whole genome shotgun sequence.
ACCESSION   DACDSY010000270 DACDSY010000000
VERSION     DACDSY010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.275"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            119..733
                     /locus_tag="IWK44_002961"
     CDS             119..733
                     /locus_tag="IWK44_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9551406.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(898..>1664)
                     /locus_tag="IWK44_002962"
     CDS             complement(898..>1664)
                     /locus_tag="IWK44_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551407.1"
                     /translation="ESNYTSSIYRLEDSNWIEISLELKTLPNSVNYIIRDITSEAQQL
                     VNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACEIGVKEFYQQEKPDLTLIFEN
                     LQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRNKLVHSPQGSKPTIEECFECY
                     MAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQITLSKELERKVESQELAIGI
                     SITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT          550 a          279 c          262 g          573 t
ORIGIN      
        1 tttcttgatt gacatttatt tacaaaaaat attgccattt aaaaagaaaa gtgacatatg
       61 gcttcctcgt gtatgattag ggtgtatttt aaatagttgc agaaagtagg taaaagtttt
      121 gagagttgca tacatacgtg tctcatcgat cgatcaaaat gaacaacgcc agattgaaga
      181 aatgaaaaaa ttcggtgcag agagaatttt catcgagaaa caatctggcg caacgattac
      241 tcatcgacct gttttccaag aagcattaga ttacgtccga gatcaagata tttttattgt
      301 ggaagctatt gatcgacttg gaagaaatta tgacgagatc atcgcttcag tcaattactt
      361 gaagaagaaa aatgtgaagc tgatcattac tagtttaccg atcatggctg aagcaattgg
      421 caatcctctc ctggataaat tcattaagga tctgatcatc caaattttgg ccatgatcgc
      481 tgaacaagaa cggactgaat cgaaacgtcg ccaggcacaa ggaattaaga tcgcaaaagc
      541 aaacggcgtc tacaaagggc ggcccaaatt atacagtgca gacgcaaaag atccccaacg
      601 tcgccttgtt tacaaaagca tcgttgaaga tcttaaaaat ggtgttgcga ttgctaagat
      661 tgctaaagac tacaatgtta cgagacaaac cgtgtataga ataaaaaaag acagcatggt
      721 caatgacaaa tagttgatca tgctgctttt attatcttct catctttaat ttcctacata
      781 taattactat ctataaaatt aagtcttact cagaacagat aaagcataaa attccaacaa
      841 accatttttt acttaactaa ataaagagtc tgttaatata atcttcactt atttacgtta
      901 atcccctact gtatttgttg gttccttatc tatattttct acaaaatcct tattatcttg
      961 tgatttagct tcgtataatt ctttgtatgt cgacacattg gttatactaa ttcctatagc
     1021 tagttcttga gactctactt ttctctctag ctcctttgac aaagtgattt gagatttatt
     1081 tggttccgtt ggagtcaatt ctatagattc ttcataaaaa tctctataca taaagctatc
     1141 attaatttta taaaaaaaat taaatgtttt tagcactgcc atataacatt caaaacattc
     1201 ttctatagtt ggtttacttc cttgaggaga atgaaccaat ttattacgtt tttctatgaa
     1261 atttttaatt tcctttctta tttctttcgg aaactcaata ttaaagtacc ctttaaacat
     1321 gctccctaat aatttgtcta cagggggaga ttgtaaattc tcaaatatta gagttaaatc
     1381 aggtttctct tgttgataaa attcctttac ccctatttca caggctgtaa ttatcgaaat
     1441 aaacctatct ctagtatttg atatattttt acttctttta aggagtataa gagaaaaggg
     1501 gatactttcg tgtccatgca tgtaattaac taattgttga gcttcagaag ttatatctct
     1561 aattatatag ttaacggaat taggaagtgt ctttaactct aatgaaattt ctatccaatt
     1621 actatcttct aatctgtata tgctcgaagt ataatttgat tcgt
//
LOCUS       DACDSY010000271         1650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.276, whole genome shotgun sequence.
ACCESSION   DACDSY010000271 DACDSY010000000
VERSION     DACDSY010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.276"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..174
                     /locus_tag="IWK44_002963"
     CDS             <1..174
                     /locus_tag="IWK44_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9551408.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
     gene            complement(309..824)
                     /locus_tag="IWK44_002964"
     CDS             complement(309..824)
                     /locus_tag="IWK44_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-keto-6-deoxy-D-glucose epimerase"
                     /protein_id="HAP9551409.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNVERLSF"
     gene            complement(1006..1617)
                     /locus_tag="IWK44_002965"
     CDS             complement(1006..1617)
                     /locus_tag="IWK44_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9551410.1"
                     /translation="MIDIISAITVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
BASE COUNT          565 a          321 c          229 g          535 t
ORIGIN      
        1 acagttgaga ctgttttttc ttccttagaa aaattaggtt gtcaaaattt caactcacgt
       61 tctgttaaag ggctagagag tagatttgaa agcatattac tggcttacag tgttctatta
      121 agtcgagcac aacgacgttt tgaaggaact ttgagatatt ctttaggata ttaaaattaa
      181 ctagcaccac gggtatatct atatctaaat ttagttttat gtattcctca tagaaataac
      241 cgagcatttg ctcggttatt gttcttctta ttttccagta tccttctttt ctccacagct
      301 cattttaatc aaaagcttaa tctttccaca tttttgtctt tctcacttat aacgaaacac
      361 tgccgactga ccggccattc aatatctacg ctttcataac ttatacattt ggcagcatta
      421 gaattgaaat cattatctac gcaccactga actagcgtat tgtcctccat agtaagatag
      481 ccatgagcat atccacgtgg gatatacgca catttaccat tgctttcact caatgtgttg
      541 acatacacac atccataagt ggaagatcct tgtcgaatat caagtgcgat attcataatt
      601 tcaccatgta ggcacatcac tatctttcct tgagcatgct ctccactctg ataatgtagc
      661 cctcgcatag tatatttatt atgactaaat ccttgattta tttgcttaat gtgaaactca
      721 ataccaatcg agaaaagagt ctcattatcc aatgcaacag cagtatatcc tctgaaatcc
      781 gaaaacttaa ttatatcaaa cacttttacc ataactacca ccatgttata tattttacca
      841 attttttaat tcgctcgacc tcatttaaag caataaaatt ccctacctct tgaagatata
      901 acaacaatgt tttagcaaaa atagatggca tgtaaagtat tcttgatgag attacacaat
      961 cctgtctgca gaaaaatatt aaccatatta aaattcaaag cagttctatt ttaagtctat
     1021 atacttattt aatcggtaga atagccaata tagcaaagca ccaattaagc cccctagggt
     1081 gttataaaac aaatccgata gttgaaatgt accaagccta aaaaacagtt gtgtaaattc
     1141 tatacttaat gagaataaaa acacatactt aatcccaaca tagatggtgc gtataatatc
     1201 acatttaatg tcacgccacc ataacaataa catgcaaaaa gggataaaca gcatcgtgtt
     1261 ctccacgcat tctgttgtca atctgctctc tcctgtagca ttatctataa tatataatga
     1321 ccactgtcca ataacgtttg aaagcgggtt catccacata tttcgattaa gcaaggtttt
     1381 aaataatatc aaagtaaaat aaaacgcaag aaaaaagagt ctgcgaaatt tactagatgc
     1441 tttaaaatat ctcaaccaga ttaatacaga atcttttaag tttttaggat ctcgagagtt
     1501 atcaactata aatagatacc agtacataaa cagatacgct gttaatacgg caaataagaa
     1561 cggctgatat aatgctgtta atacatttac cgttatagca gaaataatat ctatcattct
     1621 ctatctctct atgagcaaat gtttatttgc
//
LOCUS       DACDSY010000272         1648 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.277, whole genome shotgun sequence.
ACCESSION   DACDSY010000272 DACDSY010000000
VERSION     DACDSY010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.277"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            138..1604
                     /locus_tag="IWK44_002966"
     CDS             138..1604
                     /locus_tag="IWK44_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP9551411.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          462 a          296 c          337 g          553 t
ORIGIN      
        1 tttttatgat attgccatta aaaattactt gcttttatag aaaattagtt tatatttctt
       61 aaataagttt tttagttaaa tttttcggta aagatgtata atattttctg aaaatttaaa
      121 gaattggggt atagattatg gaaaaacagc aagacaaggg cttcttcggg cagccgaagg
      181 gattgtcaac tttgttcttc actgagatgt gggaacgatt tagttattac ggtatgcgtg
      241 cattacttat ctattatatg tacgatacag ttgccaacgg gggacttgga ttaccacgag
      301 caacagcatt agctatcatg tcgatctacg gatctttgat ctacatgtct agtatcgtcg
      361 gcggatgggt atctgaccga gtgttaggag caaaacgcac tgtattcttc ggtggagttt
      421 tcattatgct tggccacatc gtcttggctt taccatttgg tgtaagtaca ttgtttgttt
      481 cgatggcatt gattattatc ggaaccgcct ttttgaaacc aaatgtttct ggcatggtcg
      541 gacatcttta ttcaaagact gacttgcgaa gagatgcagg attttcgatt ttttacatgg
      601 gtgtcaactt aggggcatta ttagcaccac taatcgttgg tacaatcggt caagaatata
      661 actatcattt agggttctct ttagctgcta ttgggatgtt ctttggatta ttacaataca
      721 tcatccaagg acgcaagaca ttagacggag tgggtacaac accgccgaat cctttgacaa
      781 ctgaagaacg taaaggattc atccgtaact tgatcatcgc acttgttgtt atcgctgcag
      841 tattcggcgg ggcacaacta actggtcatc tgacaattga cttttttgtc aatacaatca
      901 gtgtattggg gattatctta ccgttgtatt actttatcaa aatgctgact tcaaaagatg
      961 taactgctga agaaaaacca agagtatggg cttatatccc attattcctt gcagcaatca
     1021 ttttctggtc attacaagaa caaggttcat ctatcttagc tttatttgct agtgaacgta
     1081 cacaatctac gctatttggt gtaacgatcc aagcaagctg gtatcaatca ttgaatccaa
     1141 ttttcatctt tattctgaca ccagtattcg taacgatgtg gacgaaactt ggtgaacgtc
     1201 aaccatctac agtagtgaaa ttctctcttg gacttttatt tgcaggatta tcgtacttgc
     1261 tattgatgtt gccgggtatg ctttatggta caagtggaaa agtaagtcca ttgtggttgt
     1321 tgggaagttt cttcatcgtg gttattgctg aattgtgtct ttcaccagtt ggtttgtctg
     1381 tgacaacaaa attagcacca cgagcatttg aatcacaaac aatcgctatt tggttcttag
     1441 ctgatgcttc ttcacaagca atcaatgcac aaattgcacg cttctacaca ccgggaacag
     1501 aatctgctta cttcggtatt atcggtgcag tagcaatcgt cggtggtatc ttgctattta
     1561 ctgtgaaaga accaatcaaa aaactaatgg gctctattca ttaatcaatg aacataaaaa
     1621 caggaattgc ttatttgcag cagttcct
//
LOCUS       DACDSY010000273         1636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.278, whole genome shotgun sequence.
ACCESSION   DACDSY010000273 DACDSY010000000
VERSION     DACDSY010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.278"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            135..1007
                     /locus_tag="IWK44_002967"
     CDS             135..1007
                     /locus_tag="IWK44_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9551412.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            1303..>1636
                     /locus_tag="IWK44_002968"
     CDS             1303..>1636
                     /locus_tag="IWK44_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9551413.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRR"
BASE COUNT          520 a          309 c          361 g          446 t
ORIGIN      
        1 aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca tgttactaaa
       61 taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat tctaaaataa
      121 agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa tttgtgtgtg
      181 ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca acgacacctg
      241 aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat ttagcaggga
      301 ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact tatgggtaca
      361 ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc atgaaaaaag
      421 aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat ggtgagtatg
      481 tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt acgggtgttg
      541 gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca gaaatggggc
      601 atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca tttcatggca
      661 actgtttaga agggatggcc gctggacccg ccatcgaaaa acgcttaggg aaaaaaggac
      721 aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt gctcaatgtg
      781 cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt ggtgtgatga
      841 aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc aatgggtatg
      901 tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac aacgctggaa
      961 cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg aaaaaaggct
     1021 cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat aaccagttag
     1081 gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat gagataaatt
     1141 cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt gcaatagttt
     1201 tttacacgcc gtttgtataa ttaagcgaga caaataaaaa agccatttat gttacactca
     1261 aatcgtaata cccgctaaag catacaagga gagtgaacat aaatgactta tacccatctt
     1321 acaccaaacg agcttgtaat gatagaagca tattttcatc aagaaactcc ggttgctatc
     1381 gttgcgaagc agcttaaacg tggacgccaa acaatttaca atgtctataa ctttctcaaa
     1441 tgtggtggaa cagcacttga atgctttgaa caatacaaag aaaataagcg acgttgtggg
     1501 agaaccgaaa tcatttttcc tgctgaggaa aaagaataca ttgcaaaaag atcaactgaa
     1561 ggttggaccc cagacgtcat tattggccgt gcagagcgaa ccttctcttg ttcagtaagt
     1621 accctctatc gccggt
//
LOCUS       DACDSY010000274         1611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.279, whole genome shotgun sequence.
ACCESSION   DACDSY010000274 DACDSY010000000
VERSION     DACDSY010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.279"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(68..1508)
                     /locus_tag="IWK44_002969"
                     /pseudo
     CDS             complement(68..1508)
                     /locus_tag="IWK44_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT          529 a          267 c          266 g          549 t
ORIGIN      
        1 tcatttgtta cactctcaat cttagctgtt gttctattca tcattactct tattgtaagt
       61 agttcagtca aatttgcttg ttcaattgat cgataacagt atgaattgtt tgaaaagtaa
      121 tcggatatat aattgttttc tcactaccta agccttcttg gtataaagaa ctatcgcaaa
      181 gtataatccg ctgagtacaa aagaaagtgt cgtctactat ttctctaagt tgtttacaaa
      241 tgatattact aatagaaaag tgttctccca aaatgtcttc taactgttgt cgatagtata
      301 aatacctaaa gtctgacgga gctactatga acacttcaat tggagatttc cgtttatgct
      361 catttaaaag acttaaacta atggttagtt tacgaataga attctcattc caacgcagaa
      421 ataaattgtt ttgttttcgc cattgttcaa gaatttcatg aacttctaag tacaaagcgc
      481 gttgctcttc atttaacaag taagtcttat caggttgaaa tatctgaata tcagcccaaa
      541 gtgagcggat aaacgggaga agtgctttat caaaaagtat ctgattttct tcagatatat
      601 tcagagataa gataagtttt tgactgagct caatcaccaa agggttatct gctacatggt
      661 tttgataaac aacgttcaag tctttttgta acaattcaaa gttatcgcat agataatttc
      721 tagagttgaa tagaaagtag atataaaggc attcatcttc gtcatgacat aaagagaaag
      781 aaagttgctc taatcctttc tgtattagct gaaacaaggg agtatgtcgg gcaactcttt
      841 tttcttcctg actaaaatat attgggttag tcttagatct ctgcaaaccg atagcgatac
      901 ctaataaaat aaattgtttt tcaactgtag ggtaacttct tgaaaaaaaa tgtttttcta
      961 cataatcaat caattgttga ccgctttgaa taattgattt atttagctgg atcaactctg
     1021 tccgatttgt ctcatagatt atcgttagta acaaacttcg ataatccttt tcaggaattt
     1081 caaaatggcc atcagcattt aagtactgca tttgtttaaa aaaattaatc aactcatttc
     1141 gtaagttgta aactttgctt aacgaaatga aggtagtatc agctatatta ctccaattgt
     1201 gttcccctga taaataatgt cctagtacat tgaaaaagtc agagcgctga tagatgagtt
     1261 gagtgagtgc caacctcgaa aacgaagagt cgaatgcgac tgtaaagcta cttctagatt
     1321 tctccactga aataatttgc tgatggagta tgtcggtaat gcattccaag tcgttagtga
     1381 cagacagtga gtgggcaaac gttcaattga tctgctattt cgcgaatctg aatggtccct
     1441 ttttcgtgta attgctcaca aatgtaaact tgacgaaaaa tacttttctc cagataggtt
     1501 tccaacatag tactcctcct agtcatttta tacataacta ttataactct accaatcgga
     1561 atagtcctgt aatatattga atcaaaaata taaattataa aataattaga t
//
LOCUS       DACDSY010000275         1606 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.280, whole genome shotgun sequence.
ACCESSION   DACDSY010000275 DACDSY010000000
VERSION     DACDSY010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.280"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            253..1071
                     /locus_tag="IWK44_002970"
     CDS             253..1071
                     /locus_tag="IWK44_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9551414.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1144..1503)
                     /locus_tag="IWK44_002971"
     CDS             complement(1144..1503)
                     /locus_tag="IWK44_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9551415.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          528 a          244 c          311 g          523 t
ORIGIN      
        1 tgtaccagtt atagaaaatc agttaaagta aaaatgataa aaatcttatg tcaacactga
       61 tttaggctga cctgtgggtt aggagaaaga caggtcggta gcttttaata caaaaaatgg
      121 cttgtatgta tctagatagt taactgaaat tagaaatgtt cttattttta acggagtaag
      181 aaaaataggc tgctttaggt ctgaatactt tttcaatata atgaagatat caaaggcgac
      241 aggagtgttt ttatgaataa gaatcctcat aatctaaagt tggtagcagt agatatggat
      301 ggaacatttg ctcgtactga ttacacatac gatatcccaa ggttcaaggc tgttttatcg
      361 cgaatgaaag ctgttgattg tcaatttgtt gttgcaagtg gaaatcaata ctaccaattg
      421 agatcgttat ttccaaatta ttatgatgaa ttaagttttg ttgctgaaaa tggtgctttt
      481 gttaaagata aaacagaggt actattttca gccgatatgc ctagaaaagc gatcgaggtg
      541 gtccttgatt tatgtaaaga ctatcctgaa gtaaaaaatg tgatgtgtgg tctgaatagt
      601 gcctactgtg aaagaggaaa ggtgagtcaa gcattttttg atctaaccaa tatctattac
      661 catagattag aatgggtagc tgattttgca gaagttcaag ataaaatttt aaaatttgct
      721 ccgactatgc ctgttgaaaa aaccgaattt tacgttgaat tgttccgtga aaaattagcc
      781 gggctattag tacctaccag tagtggtcat ggttctgtag acttgattct ttctgagtgc
      841 cataaaactt tcggcttgaa aatgttagtt gagcgcttgg ggattaatcc tgaccagtgc
      901 gttgcttttg gagatggggg caatgacatt gagatgttgg agtattgtgg tctgagttat
      961 gaaatggata atgcaacgga agctgttaag caagttgcta agcatcaatg tccttcaaat
     1021 gacgaagacg gtgttttagt gacgctcgat cgattgttcc ctaacacgta aacactaggt
     1081 aattcatcaa aaaacctcga tataagaaat attcgaggtt ttaaaactat atttagaaca
     1141 gatttacgaa gcgtctgatg agtcaaaata gacttggaca ttgctatcta aaataccact
     1201 ttcattcttt aaacgaattg tttgatttga tggagttacc tctaatacta aatgcccatt
     1261 aataaaggta gtatcgtatt cagatgtttt aactgttgct tcttcgcaag gtattgttaa
     1321 atcatatgct ggaatataat gttgtgtgga aactaataaa aaatgatcac caatcataca
     1381 gcatccctct gctgaagaaa attgattaga gccatcattt aaagttaaca agaatccatc
     1441 ctttaaacga ggtacttgct taaatacttc tggttgtact tctaaaaata aattatcgct
     1501 cattttcacg accaccatcg acttccacag ttgaaattga cattttattt aacacaccta
     1561 ttccccctta actataactc aattctatat aaaaaatatt ctattc
//
LOCUS       DACDSY010000276         1527 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.281, whole genome shotgun sequence.
ACCESSION   DACDSY010000276 DACDSY010000000
VERSION     DACDSY010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.281"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..365
                     /locus_tag="IWK44_002972"
     CDS             <1..365
                     /locus_tag="IWK44_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9551416.1"
                     /translation="VLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGD
                     AVDQEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELF
                     ISILKELEEHENWYAEVE"
     gene            366..914
                     /locus_tag="IWK44_002973"
     CDS             366..914
                     /locus_tag="IWK44_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551417.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            1174..1527
                     /locus_tag="IWK44_002974"
                     /pseudo
     CDS             1174..1527
                     /locus_tag="IWK44_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          601 a          187 c          328 g          411 t
ORIGIN      
        1 tcgtcttaca gacccaagta aaatcgatga gaaatgcgat gcaatatctt gagtatatgg
       61 acttttttgt gaaagaattt gatacaaatt tatatgttgc tggtgttatt cctttcatgt
      121 tggaaagcgg tgacgcagta gatcaggaaa tgtataaaca agcccaagag atttatgggg
      181 aacatttgat taaaaatgtt gtactcaaaa atgcgcgctt aaaacgatac gacggatcag
      241 gtattacgat ggagaagaca aagaaaggtc tcttaaaaca atgggatcgc aaaagccacg
      301 aattatttat cagtattttg aaagaattgg aagaacacga gaattggtat gcggaggtgg
      361 agtaattggc tgacatcaaa ggacttgata tttttgggaa caacaaagga ctcgccaaaa
      421 taaaaaaaga agcagaagca aagaaaaagc agcaagcaat tacaccatct gaaaataagc
      481 aattagaagt gcaccaagtt caagaaataa aaacttcctc caatgttgaa agacagagta
      541 gaggaagaac aaaaaaagtt ggcgcaccaa tcagaaagtt tgataaggtt tattccacca
      601 agcatccttt aaaactgtcg actttgttaa atgcgacttc cagagtttta gtagaaaagt
      661 atgagactaa tatgacaaga gatgaattgc ttagaaaagc tttagatgag tatattaaac
      721 agaatcttac gcaagaagat aagcaagatt tgtttaatga cgtggtgaaa gaactagact
      781 tgttcagaga aaaatatcca attgttccag aattagatga tcgtggagaa ataataagaa
      841 ctaccagtca gataaaacaa gataccgaag actcactgcg caaagggtgg ggaatgaata
      901 ataaagttag ataataatca aagaagaact aagtaactac ctttaaacgg tagttactta
      961 gttcttcttt gatttgcgtg aaatagaggg gatttatttt tctggttacg tgattaaaaa
     1021 atccaaaaaa tgagcatagg aacggttatg attttagggt gtgttttaat gagatattgt
     1081 gatttcatta acaagtgtat gataatctgt tgttgagtta gtaaataagt aatagagcta
     1141 actctaaaat aaaactatta ggaggagatg taggtgaaaa agtttaaaaa gaaaatatat
     1201 ttaggattgg ttgttggggt gagtatgttt gggttcatgg ctcatactaa acaagtaact
     1261 gcagatacta accaaaaaga aaggattatg agccaattag atgactctca aataagtatg
     1321 tacccaatga gctaattgtc agtttaaagg ctacacaaag aatttcaaca gagaaaaata
     1381 aaccgcaaaa tttattgggt gtaaaagtta tggtggaaaa agatttgact cttaggataa
     1441 atgaaagcga ttataataag cgagatcaag tattattagt gaaattgaac gaagaaatta
     1501 gtgtggaaga ggcagtaaga acattta
//
LOCUS       DACDSY010000277         1514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.282, whole genome shotgun sequence.
ACCESSION   DACDSY010000277 DACDSY010000000
VERSION     DACDSY010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.282"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..902
                     /locus_tag="IWK44_002975"
     CDS             <1..902
                     /locus_tag="IWK44_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP9551418.1"
                     /translation="MDNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCAT
                     LLKEAGIPNIAIVTNGSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFE
                     KAINAIKLFRELSEEVDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQ
                     NREIVPTVSQYRKLVSDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSD
                     GGLMASPYLPIQVGNIKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKG
                     HIKWLNEDIEIDILDEYKHDILI"
     gene            958..1176
                     /locus_tag="IWK44_002976"
     CDS             958..1176
                     /locus_tag="IWK44_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551419.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1247..>1514
                     /locus_tag="IWK44_002977"
     CDS             1247..>1514
                     /locus_tag="IWK44_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551420.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFK"
BASE COUNT          570 a          181 c          284 g          479 t
ORIGIN      
        1 ttatggataa tgagttgact gatgatgaac tgttagaact tacaaaagat atatgtaatt
       61 taaagccata taatatttgt ttctgtggag gagaaacttt actgagaaaa aaaatagttt
      121 tatcttgtgc cactttatta aaagaggcag gtattcctaa tattgctatt gttactaatg
      181 gatcattagt aactcaggat gttgcaaaag aattattcaa agcaggtatt aatcgatttc
      241 aagtaagttt agatggaaaa aatgctagta gccatgaagc acttagatta tatccaaaca
      301 gttttgaaaa ggcaattaat gctatcaaat tatttagaga attgtccgaa gaagtggatg
      361 ttgctttttg tcctactagt tttaattaca atcaattacg agaagtatat caactagtaa
      421 atactcttaa agtgaagtct ttaagaattc aaccatttat gcttagtggt aggggaatgc
      481 aaaataggga aatagtgcca actgtttctc aatatagaaa actagtttct gatattaact
      541 atattaaaag aaatgaagat tataccaaga tagattgggg agatccaatt gatcatatca
      601 tccgacacag aactttattg aaagattttt cgaattttgt ttctattagg tcagacggag
      661 gattaatggc atcaccttac ttgccaattc aagttggaaa tattaaatgt cacagtcttt
      721 ctaaatattg gcgagcagga ctttataaag ttttttcttt agatttagtt caagatatag
      781 ctactcaagt ttcttcaata caagatatga gtaaagaatt aaaaaaagga catattaaat
      841 ggcttaatga agatatagaa attgatattc tcgatgaata caaacatgat attttaattt
      901 aggtttatca ttggaaagtt tagataaaca aagtactttt agaaaggagg gaaaaaaatg
      961 atttctgaac ctcaaatgaa taatatgtat gaaagcccaa ctataatggg agctggtgaa
     1021 gctgcgccaa gagcttgttc agcaattatg gtgtgtgtgt tattagcatc tgcaggagta
     1081 ggtatccaag caggagcggt gtatacttat gctgtagttg ctacagtagc tgtagctgca
     1141 gccgcagttt ggaaaacagt aggattagtt ggttaggaat taataggaga ctaactgtct
     1201 cctattgatt ccaatgatta agctaaggag gaattttaag aaaataatga aatacaataa
     1261 tttaaaaccg aaattactta atgagaacat agaatatata agagaagaaa attatggata
     1321 tttaaccata gttcccaaaa atggaactga attgaaagat aacgttttca atccaacagt
     1381 aagatatata ttggctaact gtactggaaa aaacagtgta aaaaaaatag cgaatggttt
     1441 aaaaaagaag tttcctagtt tagaatatga aaaattctat caagatgttg taggaactat
     1501 taaatttttc aaac
//
LOCUS       DACDSY010000278         1509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.283, whole genome shotgun sequence.
ACCESSION   DACDSY010000278 DACDSY010000000
VERSION     DACDSY010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.283"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            101..868
                     /locus_tag="IWK44_002978"
     CDS             101..868
                     /locus_tag="IWK44_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9551421.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="IWK44_002979"
     CDS             1060..1458
                     /locus_tag="IWK44_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP9551422.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          561 a          189 c          282 g          477 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatg
//
LOCUS       DACDSY010000279         1491 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.285, whole genome shotgun sequence.
ACCESSION   DACDSY010000279 DACDSY010000000
VERSION     DACDSY010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.285"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(197..811)
                     /locus_tag="IWK44_002980"
     CDS             complement(197..811)
                     /locus_tag="IWK44_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9551423.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          493 a          286 c          240 g          472 t
ORIGIN      
        1 aactgtgaaa caacttataa aaataacaaa ctttaaacaa aacaagctta aaatcaaaga
       61 tgatccatct taaaataact aaaacaaaaa aagtagtcgt tgttaatcct cattaataat
      121 cgttttcttt ttttcaaacg ctccattttt atgtggaaca ttattttttt aaggaacttt
      181 gtcaacatgt ggataactat tttccacttg tgtcctggac tagctgtttt gctgtaaaat
      241 acaaaacttg ctgcagcaaa aagatcgttg caagagaggc tgttccgcag atcgctggat
      301 ctagatttgg caaagtcaag actgcatcat gtaaattggc gtggttgtcc aaaaccaaat
      361 cagcaaattc aaataatcgc aaaccactgc tatgttgcga tttgatactt cgtgacaaat
      421 caaatccagt aacaatgatt aatggatgac cattttcttt gatccactga gctagttcga
      481 tgattgccgg gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct gatttgacag
      541 aaagctgatc aataattgcc tgtcctgcac cttctagcgt ttcatagaca cctccggctg
      601 gattttcaat caatatactt ggtacaagat ccggggtttg atcccaaaat tcaaatgcta
      661 caccacttag tgtgcggcta gcatatatct gcaatctacc accttgatta atttgtttcg
      721 cgatccaagt gccagcttga agcatcagcg gctcttctat ccttaatttc tcttccaatt
      781 ggtgataata gtcaaaataa cataaactca tttatctctc ccgcctagct atacttgaca
      841 tgaatagaaa cagctgccta atctaaggaa gtacataaaa aaacagtagt tagacagtaa
      901 cacatgtggt ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac gcccaatacc
      961 ttgacatagc aaataaaaca acgtgcagac catggaaaat atctacatag gggtagaatt
     1021 ttcaccctag attaggtaca ataaattagg tcgatgcact gcattgacta tggcaaaatg
     1081 atcggtcagg gtgtatgtgt gtgactggtc attccctgat tcttagcgcc aactaagggt
     1141 cagggagcct ttttttattc agctgtttct atctgaattt attgtaagta atctcttatc
     1201 caagtgcaag caagaaaata caaaaaaaca ttggtatgat ttatatatat ataaataaca
     1261 taaaaaaagg aatggcttat cccgatacaa caagcattcc tttaaaaaag attcttttat
     1321 ataagtgaaa aaagcttttt tccaccaatt aaataaatct ctcatctcaa tagtttatcc
     1381 attattttat ctttttttgc gtatacctac taaatattac cgtcttcttc tcgttcttct
     1441 actttgttcg agagaatctg tactagaaaa agtagttgta tgtctacctt t
//
LOCUS       DACDSY010000280         1452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.287, whole genome shotgun sequence.
ACCESSION   DACDSY010000280 DACDSY010000000
VERSION     DACDSY010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.287"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..625)
                     /locus_tag="IWK44_002981"
     CDS             complement(<1..625)
                     /locus_tag="IWK44_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP9551424.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="IWK44_002982"
     CDS             complement(784..1050)
                     /locus_tag="IWK44_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9551425.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="IWK44_002983"
     CDS             complement(1040..1396)
                     /locus_tag="IWK44_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9551426.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aa
//
LOCUS       DACDSY010000281         1449 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.288, whole genome shotgun sequence.
ACCESSION   DACDSY010000281 DACDSY010000000
VERSION     DACDSY010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.288"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..647
                     /locus_tag="IWK44_002984"
     CDS             <1..647
                     /locus_tag="IWK44_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9551427.1"
                     /translation="EIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGKSTLL
                     SLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIRLVNK
                     SADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNLDSVT
                     AGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE"
     gene            647..>1449
                     /locus_tag="IWK44_002985"
     CDS             647..>1449
                     /locus_tag="IWK44_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9551428.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLG"
BASE COUNT          618 a          154 c          260 g          417 t
ORIGIN      
        1 tagaaataaa gaatgtagca tatagttatg caaattctaa agaaaaagtt ttgtcaggag
       61 taaatcaaaa atttgaactt gggaagtttt atgcgatagt aggaaagtca ggaacaggaa
      121 aatctacact tctttcctta cttgctggac ttgataaacc tcaaacagga aaaatattgt
      181 ttaagaatga agatatacaa aataaaggat atagtaatca tagaaaaaat aatatatctt
      241 tagtatttca aaactataat ttaatagatt atttatcacc aattgaaaat attagattag
      301 taaataaatc agcagatgaa agtatcttgt ttgaactagg tttagataaa aaacaaataa
      361 aaagaaatgt tatgaaatta tctggtggac agcaacaaag agtagctatt gctagagcat
      421 tggtatcaga tgctccaata atactagctg atgagcctac tggtaatcta gacagtgtta
      481 ctgctggaga aataattaat atattaaaga cattagctaa agatagaaat aaatgtgtaa
      541 tagttgtaac acatagtaag gaagtagcag attctgcgga tatcatttta gaactaagtg
      601 gtaaaaagtt gaaaaaagta aataaaatga atttggaggt tgaataatga taaaaaatgc
      661 atttgcttat gtaactagaa aaagtttaaa atcattaatt atcatattag ttattttatc
      721 tatgtcaact ttaagtctca ttagtttatc tattaaagat gctacggaca gagcttcaaa
      781 agaaacattt gctaatataa caaatagttt ttctatggag ataaatagac aagtaaatcc
      841 aggaacacct agaggtgggg gaaatgtaaa aggtgaagat attaaaaaga tatctcaaac
      901 agatagtata gactcctatg taaaaagaat aaacagtgtt gcagatttag ttgattatga
      961 tattattgaa actcaagaga ctcttgcgaa tcaatcacct gaaagagcaa agaattttaa
     1021 aagaacagtt atgttaactg gggttaacga ctcaacaaaa gaaacaaaat ttgtatcaga
     1081 agcatataaa ttagtagaag gaaagcattt agaaaatgaa gataaaaata aaatcttaat
     1141 gcataaagat ttagctgaaa aaaataacct taaagttgga gataagataa aaataaaatc
     1201 taatttattt gatgctgata atgagaaagg tgcagatgaa acagtagaag tagaaattaa
     1261 aggtctattc gatgggcata atagcggtgg agtaagtgca gcacaagaac tatacgaaaa
     1321 tacattaatc actgatattc atacagctgc taaagtttat ggtaataccg aagatacagc
     1381 tgtataccaa gatgcaacat tctttgtaaa aggtgataag aatcttgata gtgtaataaa
     1441 agatcttgg
//
LOCUS       DACDSY010000282         1446 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.289, whole genome shotgun sequence.
ACCESSION   DACDSY010000282 DACDSY010000000
VERSION     DACDSY010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.289"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..263)
                     /locus_tag="IWK44_002986"
     CDS             complement(<1..263)
                     /locus_tag="IWK44_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551429.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLI"
     gene            complement(563..1444)
                     /gene="galU"
                     /locus_tag="IWK44_002987"
     CDS             complement(563..1444)
                     /gene="galU"
                     /locus_tag="IWK44_002987"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9551430.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          402 a          329 c          220 g          495 t
ORIGIN      
        1 atgattagat acagtacagc aaaaaataaa gcaaatacgc aagcttccca actacctaac
       61 gccaattcaa ccatcaaggt agcaacccca acggatgctg cataccagat aaaaatcaga
      121 aacgtttctt tttttctgac catgcgaaac atgataaata gactaataag caatagtggc
      181 aacacaaata tccaattgct tccttccatc agccacttga gtatatcaag cagcgactgt
      241 gcaacagcga ctaattttcc catagtgaac acacaccttt ttttataatt aaaaaattcg
      301 cctttttatt ctactatgaa tcttctggct tggctttcat ttctcttatg taatcatttt
      361 cttttataca acttaaaaaa aaattaaacc attagcataa tattttctga atctattcac
      421 gaaagtcttt ttctatcttt cgttttccac gactcaactg atctagagtc aaaaaactaa
      481 aacgacagtt gcgcaaatga cgcacaagct gccgctttaa taacaaatag cctttcattc
      541 tcagcaggcc acttatttcc agttaacttt ctgttcttag ctttttactc aactggataa
      601 tatatgcact taatgaagtc ccaatttccg aatgttgaag tccatattca atatttgtct
      661 tcatatatcc taacttatcg cctacgtcgt atctttttcc tttaaactct ctcgcataga
      721 ccgcctctgt ctggttaaga gtctgaagtg catccgttag ctgtatctcg tttcctgctc
      781 ctggttcttg agtttctaat atctcaaata ttttaggagt cagtagataa cgtccgataa
      841 tggctaaatt acttggtgct tttcccattt caggtttttc tacaaaccct tttactctgc
      901 aacgaccctt ttcgctatct gttttaggat caataattcc gtatttagat gtttcatttt
      961 ctggtacacg catgacggca acagcggaag cttgtgtttc ttcataatcc atcatcaatt
     1021 gttgagtcaa tgggatatca tctttcatca aatcatcccc cagcatgacg acaaacggtt
     1081 catttcctat aaatgctttt gcttgaagca cggcatcccc cagtcctctt ggatgagatt
     1141 gacggatgaa gtgaagattg acttgagtcg tttcttcaac taaagcgagt aattctgttt
     1201 tttccttttc ccgcaaattg ttttccaatt cgatatttgc atcaaagtga tcttcaatgg
     1261 gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat tcccgaatcc aatgcttctt
     1321 ccacaataaa ctggatcgtt ggtttatcca cgattggaag catttctttt gccatggctt
     1381 ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg aatgactgct tttttgacct
     1441 tcactt
//
LOCUS       DACDSY010000283         1437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.290, whole genome shotgun sequence.
ACCESSION   DACDSY010000283 DACDSY010000000
VERSION     DACDSY010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.290"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            105..1406
                     /locus_tag="IWK44_002988"
     CDS             105..1406
                     /locus_tag="IWK44_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9551431.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          473 a          288 c          264 g          412 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttct
//
LOCUS       DACDSY010000284         1418 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.291, whole genome shotgun sequence.
ACCESSION   DACDSY010000284 DACDSY010000000
VERSION     DACDSY010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.291"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..617
                     /locus_tag="IWK44_002989"
     CDS             <1..617
                     /locus_tag="IWK44_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9551432.1"
                     /translation="HVLRDIDALKKDVSNFGQMFVEGNEPDSYGRNRRVFFISRDGFF
                     LLAMGFTGKKAIYFKQKYIEAFNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSM
                     LKDTMRISGQQEFEIKQKGNMKVMGVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPR
                     YGELPRKRFDDAVSFIEMWLPETAIRMEIDQLNRQQRLFGDDNE"
     gene            795..1136
                     /locus_tag="IWK44_002990"
     CDS             795..1136
                     /locus_tag="IWK44_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551433.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1129..>1418
                     /locus_tag="IWK44_002991"
     CDS             1129..>1418
                     /locus_tag="IWK44_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9551434.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT          527 a          210 c          299 g          382 t
ORIGIN      
        1 aacatgtttt aagagatatt gatgcactaa aaaaagatgt gtccaatttt ggacagatgt
       61 ttgtagaagg taatgaacca gattcatatg gcagaaatcg acgagttttc ttcattagta
      121 gagatggatt tttcttgctg gctatggggt ttacaggaaa gaaagctatc tacttcaaac
      181 aaaaatacat tgaagcattc aacgaaatgg aagatgttat tcgcaagaat actgttcctc
      241 aaacaattga agatatgatg atctatcaat tagaagaaat gaaagatgtt aaaaaagatg
      301 tttccatgct taaagatact atgcgaatta gcggacaaca agagtttgaa attaagcaaa
      361 aaggaaatat gaaagttatg ggagttctag ggggaaaaga aagccgagct tatgaagaaa
      421 tcagcaaaaa agtattctca aaattttggt ctgaatttaa acgtaccttt tcaatcccaa
      481 gatatggcga gttacctcgt aagagattcg atgatgctgt ttcatttatt gaaatgtggt
      541 tgccagaaac tgcgatccgt atggaaattg atcaactgaa cagacaacag agactttttg
      601 gtgatgacaa tgaatagagc tgaagcgcta agaataggga cggcaattgc taattgctgg
      661 tggaaatact ataaaccaat catcctaagc caacaacata ttgacaagca aaaagcatgg
      721 caacaaataa aaaagtgact ccgccggcaa gcaaagagtc acaaagaaaa cacatcataa
      781 ggagatttta gcatatggaa aaagaacttt ccactctaga tcaatatttg attgatcctg
      841 attggggcaa gccgaaaatt gaggaaacaa gtggtcgaaa aatcagacga aatcttttga
      901 cgaatgaaga actagcttgg gatcaagatg atttaggcaa ccatgtaact atttgggatc
      961 atgtttatct tatccatcta tcgaagcatt cgaataaacc tgaatatatt tacgtcatcg
     1021 aagatggctt gattgatgcg ctagaagagt acgacagaga taacttgatt gatatctctt
     1081 attacggacc aggtaagaaa tacattgctg aaatggaggc agaatttgat gagtgaaatc
     1141 aaagggacaa cgaactttga aaaacttttt agtcgtaagt taaataaaat tctcaagaaa
     1201 aaaggaaatt ttgattattt atcttgggct cacgcgtggg agattatgaa aaagaatgat
     1261 ccacaggcaa cggtaactat taatgagtac aaacactaca gggttgtttc tggaactcat
     1321 caagactttc ttgttgagga atataaacct tttcttatgg atgaaactgg gacttatgta
     1381 tctgtctcag taacggttaa aggacacacg gaaaccga
//
LOCUS       DACDSY010000285         1417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.292, whole genome shotgun sequence.
ACCESSION   DACDSY010000285 DACDSY010000000
VERSION     DACDSY010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.292"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IWK44_002992"
     CDS             complement(150..1337)
                     /locus_tag="IWK44_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP9551435.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DACDSY010000286         1407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.293, whole genome shotgun sequence.
ACCESSION   DACDSY010000286 DACDSY010000000
VERSION     DACDSY010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.293"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            77..1387
                     /locus_tag="IWK44_002993"
                     /pseudo
     CDS             77..1387
                     /locus_tag="IWK44_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
BASE COUNT          590 a          115 c          238 g          464 t
ORIGIN      
        1 attattggga atcccagctt aaatcataga taccgtaagg gattttattc tttatttaaa
       61 actttgcaac agaaccgaat cattaaaaaa acattataca gagccttggg aagatgaagt
      121 ttttagagta attattgaat ataacaatgt tcctattgga tatggacaaa tatataaaat
      181 gtatgatgag ttatatactg attatcatta tccaaaaact gatgagatag tctatggtat
      241 ggatcaattt ataggagagc caaattattg gagtaaagga attggtacaa gatatattaa
      301 attgattttt gaatttttga aaaaagaaag aaatgctaat gcagttattt tagaccctca
      361 taaaaataat ccaagagcaa taagggcata ccaaaaatct ggttttagaa ttattgaaga
      421 tttgccagaa catgaattac acgagggcaa aaaagaagat tgttatttaa tggaatatag
      481 atatgatgat aatgccacaa atgttaaggc aatgaaatat ttaattgagc attactttga
      541 taatttcaaa gtagatagta ttgaaataat cggtagtggt tatgatagtg tggcatattt
      601 agttaataat gaatacattt ttaaaacaaa atttagtact aataagaaaa aaggttatgc
      661 aaaagaaaaa gcaatatata attttttaaa tacaaattta gaaactaatg taaaaattcc
      721 taatattgaa tattcgtata ttagtgatga attatctata ctaggttata aagaaattaa
      781 aggaactttt ttaacaccag aaatttattc tactatgtca gaagaagaac aaaatttgtt
      841 aaaacgagat attgccagtt ttttaagaca aatgcacggt ttagattata cagatattag
      901 tgaatgtact attgataata aacaaaatgt attagaagag tatatattgt tgcgtgaaac
      961 tatttataat gatttaactg atatagaaaa agattatata gaaagtttta tggaaagact
     1021 aaatgcaaca acagtttttg agggtaaaaa gtgtttatgc cataatgatt ttagttgtaa
     1081 tcatctattg ttagatggca ataatagatt aactggaata attgattttg gagattctgg
     1141 aattatagat gaatattgtg attttatata cttacttgaa gatagtgaag aagaaatagg
     1201 aacaaatttt ggagaagata tattaagaat gtatggaaat atagatattg agaaagcaaa
     1261 agaatatcaa gatatagttg aagaatatta tcctattgaa actattgttt atggaattaa
     1321 aaatattaaa caggaattta tcgaaaatgg tagaaaagaa atttataaaa ggacttataa
     1381 agattgatta tataatatat gaaaagc
//
LOCUS       DACDSY010000287         1382 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.294, whole genome shotgun sequence.
ACCESSION   DACDSY010000287 DACDSY010000000
VERSION     DACDSY010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.294"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1350
                     /locus_tag="IWK44_002994"
     CDS             <1..1350
                     /locus_tag="IWK44_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9551436.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT          553 a          219 c          301 g          309 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 ga
//
LOCUS       DACDSY010000288         1373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.295, whole genome shotgun sequence.
ACCESSION   DACDSY010000288 DACDSY010000000
VERSION     DACDSY010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.295"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            191..445
                     /locus_tag="IWK44_002995"
     CDS             191..445
                     /locus_tag="IWK44_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9551437.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IWK44_002996"
     CDS             446..763
                     /locus_tag="IWK44_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9551438.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          418 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat ata
//
LOCUS       DACDSY010000289         1372 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.296, whole genome shotgun sequence.
ACCESSION   DACDSY010000289 DACDSY010000000
VERSION     DACDSY010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.296"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            122..>1372
                     /locus_tag="IWK44_002997"
     CDS             122..>1372
                     /locus_tag="IWK44_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9551439.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          428 a          271 c          261 g          412 t
ORIGIN      
        1 gctctatact ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata
       61 gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa
      121 gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt
      181 cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg
      241 aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat
      301 cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac
      361 ttatctggag ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc
      421 ggatacctct gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat
      481 tgctcgctta aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac
      541 ttcttcggtc tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac
      601 tttgccaaag gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat
      661 gagttttatc atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg
      721 cttgccttat ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca
      781 attgattgtt attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa
      841 tgctcagttg atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa
      901 tcatcgagtg aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt
      961 agggaaaaaa ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta
     1021 tgaaaaacgg gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga
     1081 tcgactgtta gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct
     1141 ttatgcggta aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag
     1201 cggtctgcca gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat
     1261 ccaaaatgcg cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat
     1321 caaagtatta aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DACDSY010000290         1368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.298, whole genome shotgun sequence.
ACCESSION   DACDSY010000290 DACDSY010000000
VERSION     DACDSY010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.298"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(277..1302)
                     /locus_tag="IWK44_002998"
     CDS             complement(277..1302)
                     /locus_tag="IWK44_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9551440.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT          408 a          230 c          268 g          462 t
ORIGIN      
        1 agtatcccca aataacatgg attgaaataa gggtaaatgt gtttaaaaaa acgaactata
       61 aaaacttata cacaacgtgg ttaaaaatag ttttttcttc cctgtggaaa aaatagttta
      121 acacaaacat tatagttatc cacagctatc taaaacagat ggaaaataag aaaaattcgc
      181 taaaataccg ggagggggta acgagggatg agtgattgaa aaaagaacag agtaactgac
      241 ttatcctcag tgtttctgtt cttttataat tctgttctac atttcttgtt gttggatcga
      301 atctgtgaga gtaatctcgg ttgaaagaat gattcttttt tcttcagggt attggtggtg
      361 gattagttgg aataaaagtt ctgtggcttt gattcctaac tgatttgtcg gaatgtctat
      421 ccgagtcaac tgtggcgaaa atagggagga caataaggga tcactgaatc ctgcgatcga
      481 aagatcatca ggtatctgct ttccagattg ttctgccact ttgtaaatac tcaatgattt
      541 taatgcatcg gtagaaaaaa tcgcagttgg tctttctcgt agcttcatta aatcattcat
      601 tgctgccatg ctatcttcta agcgaaagcc gccgtctata atccattcat tacggatatc
      661 taggtggtga tcaaataagc attgacggta accattgaga cgatcattgg atacataaac
      721 ttctgtcgga gcatggagac agccgatatg acggtgtcca ttcttgatca gtgtttccac
      781 caaatcgtaa ctatcctgga aattatccgt gtccactgag tagatgttcg tgtagtcgtt
      841 tccgactttt ccgattacca caataggaat attatattga tccaattcaa aaagaagtgt
      901 ttcatccatc gtagagctta aaataataat tcctcggatc attttttctt ggatcttttt
      961 cgcgcatttt tctagctctt cttctggtgt ttttgccgtt tgtagaatca agtcgaagtt
     1021 attttgttca gaaacggcag aaatggattg aataacatct gaaaaaaaag gattgccata
     1081 ggtcgtatga gaagtacgat gagataatgc tagaatcgcg tcaaaaccat ttcctgacag
     1141 tcctcttgca attttgctcg gttcataatt caattctttg attgcttgaa gtacacttgc
     1201 ttttgcttta tcagagatat ttggctggtt atttaatact ctggagacag ttgattttga
     1261 gacattagct tttttcgcaa tgtcataaat agtaggtttc aattttatat gcacctcctt
     1321 tttgggagcg ttttagaata attccatagt acaatagaca ttcttttt
//
LOCUS       DACDSY010000291         1364 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.299, whole genome shotgun sequence.
ACCESSION   DACDSY010000291 DACDSY010000000
VERSION     DACDSY010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.299"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            82..>1364
                     /locus_tag="IWK44_002999"
     CDS             82..>1364
                     /locus_tag="IWK44_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP9551441.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DACDSY010000292         1364 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.300, whole genome shotgun sequence.
ACCESSION   DACDSY010000292 DACDSY010000000
VERSION     DACDSY010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.300"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            82..>1364
                     /locus_tag="IWK44_003000"
     CDS             82..>1364
                     /locus_tag="IWK44_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP9551442.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          463 a          255 c          259 g          387 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcgatgatct atccttacac caatggaaaa
     1261 atagaagcga agaataccca tataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DACDSY010000293         1357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.301, whole genome shotgun sequence.
ACCESSION   DACDSY010000293 DACDSY010000000
VERSION     DACDSY010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.301"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            98..1267
                     /locus_tag="IWK44_003001"
     CDS             98..1267
                     /locus_tag="IWK44_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9551443.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          494 a          201 c          220 g          442 t
ORIGIN      
        1 atattttgtg taaatagata tttagggtag aaaaaaagaa agtccattct gtaaaattaa
       61 agttaccaca accgaaattt tataggagga ctttctcatg cccaatttta ctacagaaat
      121 tatggaaaca ctaatcaata aaggtgattt agatgaatta tttcgatgtc atttagaatt
      181 agctgttaat tcattattgc aggctgaatt aacagctttt cttgattatg aaaaatatga
      241 tcgtgctggg ttcaattcag gtaattcccg taatgggaat tattcacgtt cattcagaac
      301 agagtatgga gaattaaatt tggtaattcc tagagataga aatggtaagt tttctcaaca
      361 aactttacct gcttataaaa gaaccaatga ctctttagaa accactatta ttcagctatt
      421 tcaaaaagga attactatgt ctgaaatctc tgaattgatt gaaaaaatgt acgggcatca
      481 ctatacgcca caaaccattt caaatatgac taaaatagtt tctgaagata ttattgcctt
      541 taaagaaagg tccttagaat ccagatactc tgtcattttt atggatgcta ctcacattcc
      601 tttaaaaaga caaactgttt caaaagaagc agtttatatt gtaattggca ttcgattaga
      661 tggaaccaaa gaggttcttg ggtttagtct tgctccaact gaatctgctt atgtttggaa
      721 agagattctt caagacctaa aagaccgtgg tttaaaagag gttttattag tcgtaactga
      781 tggtttaagt ggcattaacg atagtattca tagtgtttat ccaaatgctc aatttcaaca
      841 atgttgtgtc catatatcta gaaatatcgc tcataaggtt cgtgttagtg atcgacaaga
      901 aatctgtagc gatttcaaat tagtttatca agcaagttca aaagaagaag caaataatca
      961 aataaggttt atgatagata aatggaaaaa gcagtatcca cgagtggtta aattgctcat
     1021 gaatcctgct atattaacct tttataattt ccctccgtca atcagaagaa ctatttattc
     1081 aactaatttg attgaaggtt ttaacaaaca attaaagaaa tataccaaaa gaaaagagca
     1141 atttcctaat gaagaatctc tggagagatt tctagtatct caattcaaca actataatca
     1201 aaagtttcta tgccgagttc ataaaggttt taaagaaata catgatacat tagaatcaat
     1261 gttttaactt gatcaacaga atgggctttt ccatttacac aaaattattg acgctcccga
     1321 caaaattatg atcttgcgta aagttataaa atattaa
//
LOCUS       DACDSY010000294         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.302, whole genome shotgun sequence.
ACCESSION   DACDSY010000294 DACDSY010000000
VERSION     DACDSY010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.302"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>1355
                     /locus_tag="IWK44_003002"
     rRNA            <1..>1355
                     /locus_tag="IWK44_003002"
                     /product="23S ribosomal RNA"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DACDSY010000295         1329 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.304, whole genome shotgun sequence.
ACCESSION   DACDSY010000295 DACDSY010000000
VERSION     DACDSY010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.304"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            283..825
                     /locus_tag="IWK44_003003"
     CDS             283..825
                     /locus_tag="IWK44_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551444.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            915..>1329
                     /locus_tag="IWK44_003004"
     CDS             915..>1329
                     /locus_tag="IWK44_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP9551445.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          534 a          158 c          249 g          388 t
ORIGIN      
        1 cttgacatga aaaatcctcc agtgtgtttt ttattattct acacacctta ttttttctgt
       61 ctagtctagt gtagccgatt caaattgatg aagcagatcc caaagggctg cttcatcaat
      121 tttttcgtaa gtgtccgtac acttactgtg cttgctcata tattaatcga tcatatacta
      181 atcgtgtaag aacaacttta agcataatac ttatattgtt ataccatagt ttggaaaaat
      241 aaaaaattat atttcatcaa cgtttcataa aaggtgtgta gaatgttatt gaaagtagct
      301 ataaaatgct attgtgtaaa aaataaagga ggaataattt atgaaaaaat agtaccaaga
      361 ggagcatgcc tattacttgc attatgtttg agcttaagcg cagttgggtg tcaaaaaagt
      421 aatgaaaaca ataaaataaa agaaggacaa tccataagtt ctgaagagga aggcatgtca
      481 attaatgcaa atggcaaaaa tgaaactttt aagccatcag attatacatt ggaggcaaag
      541 aaagaatatg tttatgaata tttagggctt aaatttaaac tctcagacaa atttagaaat
      601 tatatagctg ataaaaagat agcgatgtta gatgatcaaa gtcctataga taaagaacta
      661 aagtatgcta ttttgacatt tgagaaaatg acagaagaac aaaagaatgc agttatagaa
      721 aagatgggcg atggatataa aaattggcaa aatgagcttg aaagaattgg gactattggt
      781 atatttgaaa aaaatacatc agaagaaaaa aatctaaaat tataaaatgt gatactcata
      841 ctaaaatagg agtttcaagt gatggaaaat acgattgcta tttaagcaca aatagcggag
      901 ctgaaagtaa tcttctggat gaattaaaaa gaactgagat tcaaattata gataaaaaag
      961 aacgtcctaa aaatggtttt gtgctatcag aaaaaactga tttagaaaat acagaagcat
     1021 ttaacaaaga atcggttaaa gatttacgta aactaccaac aaaggacata aatggcaagg
     1081 attttacaag taaagatttt gagaagtatg atcttactat ggtaaatgta tttgcaactt
     1141 ggtgtacagc ttgtgtaaag gaaattcctg atttggttga ggttcagaat gaaatgaaaa
     1201 gcaagggtgt aaatatcgtt ggagttgtta cagatcctgt tgatgataac ggtgaaaaca
     1261 aggaagcaat tgaaaagtca aagttaatac atgaaaagac aaaggcttca tatcctttct
     1321 taatgccag
//
LOCUS       DACDSY010000296         1320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.305, whole genome shotgun sequence.
ACCESSION   DACDSY010000296 DACDSY010000000
VERSION     DACDSY010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.305"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            122..529
                     /locus_tag="IWK44_003005"
     CDS             122..529
                     /locus_tag="IWK44_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551446.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            543..944
                     /locus_tag="IWK44_003006"
     CDS             543..944
                     /locus_tag="IWK44_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551447.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            946..1317
                     /locus_tag="IWK44_003007"
     CDS             946..1317
                     /locus_tag="IWK44_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551448.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          510 a          167 c          231 g          412 t
ORIGIN      
        1 tatgcttaca aaattagcta atatttccgt tgacaaagct ttgttactat tgttaaacta
       61 aattgactag caaaagtgaa acataaattt attaaataaa tgtaaaaatt aggagaaatc
      121 aatgtcttat agagtacaat taataatttc agaagatgta gaaagtcagc aatttggcac
      181 taatgttatt aataaagtaa ttaatccagc tctttctatt aatgcgcctt taattcctac
      241 tgctttatca ttttctgtaa cagctgtggt gtctgagata gaggatacag aaaagataaa
      301 aatagtagaa attgaagttt taaacaagaa tgaaaaacaa attttttcta cgggtgaagt
      361 atcggtaagc ttgcctcctc aagttaacga tataaacttt aatataaacg ccagaaatgt
      421 cttggttgaa gaagcaggag agcattatgc tgttgttaaa tttaatggaa ctgagattgg
      481 aaggcaaatc tttgatatca aggtcaacaa accagtggaa aaaaattaag gagacgatac
      541 ggatgcttga tatagtacca tcaaatacac atcggagtgg tagcttaata aattttgctg
      601 cgatttttgc ttgcgcatcg gtaattctag catctccagt tgttgtggat gcaagttcta
      661 ctccgaaaac taaaaatgat aatcaacttg taattactac aaaaaatgag attaacacaa
      721 tttcaagtaa tgatggcaaa atttttgatg tgataaatac ggttttaaaa agaaaagctg
      781 aaatcaatat aaaatatgat gaagatttaa acttgtattt ctttgttatc aaaacaacat
      841 ctgaattgtt ttcctcagat tacgatgttt tggatactct cgataatgta ttatctgact
      901 ataaatatat ggggaaatct gtagtagcaa ctttggggga ataaaatgtt tgactttaat
      961 gaatatttat ctatttccaa acaaattgaa tcagatgata aatataattc taaagaatct
     1021 tatagaagaa ctgctgtttc aagagcatac tacagtgctt ttaaaaaatc tgatgagtat
     1081 ttgaaagaaa attatgatat tatttataat ggaagtagcg gaaaaggaag ccaccaaacc
     1141 gtttggaatt tgttttcaac agttaaggaa ttgaatacct taggaataca taatagtggg
     1201 tatagaatgt tagagaaaag aaaatgtgct gactatattc ctaatgaaac cattacgaaa
     1261 acagatatgg cgctgatgaa tcgagaagca gaaaaaataa taaacaaact cacctaatga
//
LOCUS       DACDSY010000297         1318 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.306, whole genome shotgun sequence.
ACCESSION   DACDSY010000297 DACDSY010000000
VERSION     DACDSY010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.306"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            188..391
                     /locus_tag="IWK44_003008"
     CDS             188..391
                     /locus_tag="IWK44_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551449.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            602..1186
                     /locus_tag="IWK44_003009"
     CDS             602..1186
                     /locus_tag="IWK44_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9551450.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          465 a          183 c          252 g          418 t
ORIGIN      
        1 agtgacagga acgatgattt ttgataaagt tggttggcct tctagacctc ctcaatcacg
       61 gaattggaaa gaaaaaagaa atggttattg gtactatgga aaattgtatt tacaaagtta
      121 taaatcagct ggaaacaatt ggattgcgac ttattcagga accttaacta gaaagtaggg
      181 attttttatg ccagaatttg tgaacacacc aaatttttat caagatagcg atattctaag
      241 taaaattaat ttaccgaaag aagaatcagc tttacaagtt cagcgaactt tacaggagac
      301 acaaaaaatt caaaaatcaa tttctaatct agaagattct gaagaaaaaa gaaaaaaaag
      361 agaccaaaaa acatatcaaa aagaaaaata atgattttaa aaaaagagtg tctagtcgac
      421 attctttttt tctaattgaa agtgttcata aaatcaccca tttctggaca cttccttttt
      481 ttgctttatt tttcaaaaaa acaattcatt tcatgttcat aaacccataa caatatctat
      541 gttcagttaa actatatgga taaagttatg ttacaataaa attaatgaag gagtgatttt
      601 tttgaaaata ggatacgccc gggtatctac tggtttacaa aatttggatt tacaagaaga
      661 cagcctaaat aaatttggtt gtgaaaaaat ttttactgat catatgagtg gggcaaaaag
      721 taatcgacca ggattagaaa tggccattga ttttgttcgc tctggcgata cacttgtcgt
      781 ttggcgctta gatcgtttag gtagaaatat ggaagatttg atttcaatcg tcaatcgact
      841 gaatgagcgt ggtgtgagtt ttcatagtat tcaagaaaat attacgatgg acaaatcaag
      901 ttctacagga cagctcatgt ttcatttgtt tgcggcattt gcagaatttg agcgtaattt
      961 gatcctggaa cgatccgcag caggacgagc agctgctcgt gcaagaggac ggtttggtgg
     1021 tcgtcctgaa aaattgtctg aacaagattt ggaattactg aagactttag tggatagcgg
     1081 tacacctatt aagactattg cggaacggtg gaacgtctca agaaccacca tttatcgtta
     1141 tttggcaaaa atggaggaga aaaatggaaa gacaaattct cattagtttt tgtcatttaa
     1201 tgctgaaaat ttttgccatt tatctagaaa agtgacaata gtatgagaat tttttgtaag
     1261 tgtctactga taacttattc tcaaatgttg taaggatttt attcatttgt taatatta
//
LOCUS       DACDSY010000298         1314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.307, whole genome shotgun sequence.
ACCESSION   DACDSY010000298 DACDSY010000000
VERSION     DACDSY010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.307"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..848
                     /locus_tag="IWK44_003010"
     CDS             <1..848
                     /locus_tag="IWK44_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9551451.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          429 a          240 c          254 g          391 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttttta acggaaatct atcctatact aaat
//
LOCUS       DACDSY010000299         1292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.308, whole genome shotgun sequence.
ACCESSION   DACDSY010000299 DACDSY010000000
VERSION     DACDSY010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.308"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(13..1287)
                     /locus_tag="IWK44_003011"
     CDS             complement(13..1287)
                     /locus_tag="IWK44_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9551452.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT          369 a          246 c          264 g          413 t
ORIGIN      
        1 ccgcatattt ttttagtttt caaagatcag accttgttgt aaatagatcc gtgcccgaaa
       61 gttataaaag ttgcgataac caaacgcaat tcgtttaatc agcttgatct tgttattcag
      121 cccctcagtg acgccgtttg agtgggacac cttaaaggca tttgaaatac ccgattggta
      181 gcaatggaag atcgtgaatt tctttttaaa cgactgaggt aacgtagatg aaattgtttt
      241 aagggtttct tgaaagcctt ccgtacctcg tttaacgaaa taatagcgta ataattgaca
      301 aaaatgatac gcagttcgca attcttcgtt gtagcttaac agctcatcaa cgatttctgt
      361 ttgactaatt ggtcgtttaa acagtcgatg ataatgccgt ttgagtggct cccatgtcgt
      421 ggaatctttg agtaagagtt tccaatatcg cttgagccga cggtacttct tctgattttc
      481 agagtgtgag gttcgaaaac gattcatgat ctggacacgt aattgattga agctgcgatt
      541 gatgtgttgg acaatatgaa agcggtcagt tacgatctcc gcatttggaa agactgtttt
      601 aagcaactga caatagctgg cattcatgtc catcactagg tacttgacct ttaaacgggc
      661 ttttctagga aagtgcagga agtattgagt tagtttgtaa agtctgcgat cttcaaggat
      721 cgtaaatagc tgatgggttg tcccatcaac acaaataaaa ctcatggaac cagaaacaga
      781 cttcattgat ttgaactcat ccatacacaa caccgtcgga aggaagttca tacgtgactg
      841 gtaatgcttc gttgcttcac gcaagatccg tgtcaccgtc ccatccgaga cgaagtactt
      901 cttagcgatt agtttacgtg attgattttc gtacagttcc atgagaattg cctgcttcag
      961 ttcctttgtt agttgatgat gttcatcaac taagtccgtt ttcgcagaaa aggtccttcc
     1021 acaatcatgg cagagaaaac gggtacgttt taattctaaa atagttaact tttctttgac
     1081 tttcaatatc tgagtgcgtg tcgtatagga accgttgcgg ataatacgaa ccgatcgaca
     1141 gtgttcacaa taggaaggag agtcatccaa ttttccttta ataaactgcg cttgaacgcc
     1201 tttatattct ttttcaatca gccattcttc atcaaaaaat aaatgtttat ctgttaatcc
     1261 gagtaatttt ctagtatgat tatccattga ga
//
LOCUS       DACDSY010000300         1291 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.309, whole genome shotgun sequence.
ACCESSION   DACDSY010000300 DACDSY010000000
VERSION     DACDSY010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.309"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(99..425)
                     /locus_tag="IWK44_003012"
     CDS             complement(99..425)
                     /locus_tag="IWK44_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551453.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(412..1200)
                     /locus_tag="IWK44_003013"
     CDS             complement(412..1200)
                     /locus_tag="IWK44_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9551454.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT          365 a          227 c          185 g          514 t
ORIGIN      
        1 tattacaata tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa
       61 atatcacgat attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt
      121 gctagtttat atccttcaat caagtattct tcaggtatgg attttaatag cgcatcttct
      181 ataacgtctg gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac
      241 attagaattg gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt
      301 atgactttac ttatattttt cttggttaat tcgatttctt ctacttcttt tggtttgtag
      361 ttctttttat ttttatttcc tagtagtcta cttactgcag cttgactgcg attatcttct
      421 gccatcaaaa tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc
      481 agctgttgag ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac
      541 ggttattgtt ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc
      601 ggttgctgca gtcgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag
      661 gactcctcgt acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact
      721 taactttatt agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc
      781 agaaacagta aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat
      841 ataatcatag ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac
      901 ttgagtaaat tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc
      961 tttatagtcc agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt
     1021 aggttggttt gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc
     1081 aatgtccact aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac
     1141 gctagtcgta gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat
     1201 taaaatatcc cccattcatc ttatattata atataattat atcataatat tgtgatattg
     1261 caatatcgta tatgcattat attgtaatat t
//
LOCUS       DACDSY010000301         1290 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.310, whole genome shotgun sequence.
ACCESSION   DACDSY010000301 DACDSY010000000
VERSION     DACDSY010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.310"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(27..>1290)
                     /locus_tag="IWK44_003014"
     CDS             complement(27..>1290)
                     /locus_tag="IWK44_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9551455.1"
                     /translation="QTKKLAVFSKLKLCYLKESNRFNYLMLLINYPQTAVPKKYLLEQ
                     LYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIRLYLYFFLSDTFGGND
                     WPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQQAYFPKTSSEITELM
                     DLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQISFRQKQEIGFLFST
                     SKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFFELLGESSSSFSTLCF
                     PEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLLIHL
                     QITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHEKISDETETFYLDTTH
                     NTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          418 a          178 c          233 g          461 t
ORIGIN      
        1 aataagagga tgtgacaaga attttgtcac atcctctttg ttttgttctc ataacctttt
       61 ttcatataca gttcatgaac cgcttttatt aacttgccaa ataacactgt attgtgcgtt
      121 gtatccagat agaaagtctc cgtttcatct gaaattttct catgctgatc tgtaatgatc
      181 aaatcagcat gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa taactgctgc
      241 aaatggcttt gaagatgata atttcctgta aactgtttgg ttatctgcaa atggatgagt
      301 aactgctctt tcgtttctgt ttgtagtaag tgttggatca attgactaag gtattgactg
      361 atttggtttc tttccccatt ttttgaaaaa attgtatcga ttttttctct taatttcttt
      421 tcctgtataa aagaactaga atgattggac gtggcttctg gaaagcaaag tgtagaaaaa
      481 ctactgctgg attcacccaa cagttcgaaa aaaacaactg ccaacacaag aaaataaaaa
      541 taaattggta ttttattttc taaatggttg agaggaaact gttgagttaa gtaagtcatc
      601 aattgaccga cttgtcgaca aataaggttt ttgcttgtgg aaaataaaaa accaatctcc
      661 tgtttttgtc gaaaagagat ttgatttgga aaaaaacaat agaaaaaata attaaagtag
      721 gctctctctg tttctgtctc ttcacaaggg cgactcactg aaaaagaagt aaagtattct
      781 gaataatcat gtgtatgctg caataagtcc atcaattcag tgatttcaga agaagttttt
      841 ggaaaatatg cctgttgttt gtttcgtaat atgaataaag ttaacagcaa gtagatattt
      901 ttttcttgtt ttgaaatttt ttctatttgc tcatctgtac tgttcatttt tagtgcattt
      961 aattcaagtg aggaaaatgg ccaatcgtta cctccaaatg tatctgaaag aaaaaagtaa
     1021 agatagagac ggatagataa ctcatttcct tcaagagtac agcaattttt tacagatagg
     1081 atccgtaaat ggaatttttt taaggaatga ttgagctgtt ttgtcagttt ttcgagatag
     1141 gagggcgata tataaagttg ttcaagtaaa tatttttttg gtacagcagt ttgtggataa
     1201 ttgattagta acatcaaata gttaaaacga ttggattctt ttaggtagca taattttaac
     1261 ttagaaaaaa cagctaactt tttagtttga
//
LOCUS       DACDSY010000302         1288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.311, whole genome shotgun sequence.
ACCESSION   DACDSY010000302 DACDSY010000000
VERSION     DACDSY010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.311"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(61..279)
                     /locus_tag="IWK44_003015"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="IWK44_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="IWK44_003016"
     CDS             complement(545..739)
                     /locus_tag="IWK44_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551456.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          455 a          217 c          175 g          441 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaa
//
LOCUS       DACDSY010000303         1284 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.312, whole genome shotgun sequence.
ACCESSION   DACDSY010000303 DACDSY010000000
VERSION     DACDSY010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.312"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..174
                     /locus_tag="IWK44_003017"
     CDS             <1..174
                     /locus_tag="IWK44_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9551457.1"
                     /translation="KKSNYIFDGQCGIIKMRDYLILDIDSEEDFKWLEFLFPKFLEDS
                     SELQDIYNYLYRR"
     gene            179..1126
                     /locus_tag="IWK44_003018"
     CDS             179..1126
                     /locus_tag="IWK44_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9551458.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          428 a          179 c          274 g          403 t
ORIGIN      
        1 aaaaaaagta attatatttt tgatgggcaa tgtgggataa taaaaatgag agattatcta
       61 attttagata ttgatagtga agaagatttt aaatggttag aatttttgtt tcctaaattt
      121 ttagaagatt cttcggagct tcaagatatc tataattatt tatatcggag gtaaaaggat
      181 gagctgttat cttatcaccg gtggggctgg cttcattggt tctaccttag ccaatcattt
      241 aggagaagaa catgtggtaa tcgtcgtcga tgacttatca atgggaaaag tagaaaatct
      301 tgatatgacg aaaaatatca cttttattga gggagatgtc gctgagaaat ccttgatgga
      361 agagatcatg aaagctaata aatttgatta tatctttcat ttagcagcag ttgctagtgt
      421 ggcagattca gttgcccgac cagtagaaac acatcgtgtg aattttgaaa gtgtattaat
      481 gattttagaa ttgatccgca agtatcaacc tgatttaaaa cggatcgtgt ttagttcttc
      541 agcggctgtt tatggggatg aaccgacttt accaaaaaaa gaagagtccg tgattcgccc
      601 attgacgcct tatgcggttg ataaattcgc tgcagaacaa tatgtgctag attattgtca
      661 tttatatgat gtacccggta gcgcagttcg ttttttcaat gtctatggtc caaatcaaaa
      721 tccgaattct ccatacagtg gtgtgatttc tattctcgta gatcgctata agaaacaaat
      781 cgtaggagaa gcaacttcat ttacattatt tggtgatgga agccagtcta gagactttgt
      841 ttatatagat gatgtaatcc aagcattact tttagttgcg aatgaggaag ctgcactggg
      901 gaaacaattc aatgttggaa cgggtaaagc aaccacactt ttggaattga ttcatgcaat
      961 caatgaaatt ctcggtgtag aattgacact tgaatacaaa gaagagcgtt cgggtgatat
     1021 ccatgactct ctagcagata ttactaagat ccagtcaatt ggctatcaac cgaaatatgg
     1081 tattttaagt ggaatgacta attatttgaa aactgaaatc aattaaaaca tactaagata
     1141 agattagagg gtaaattcag gctctttgtc aataaggact gatgagtttt gtaaaatcaa
     1201 atctgggata gaagggctct ttgtcaaatg atgttggtaa agagaaatct tagaataatt
     1261 tggacagaac agaaagaaga aaac
//
LOCUS       DACDSY010000304         1266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.314, whole genome shotgun sequence.
ACCESSION   DACDSY010000304 DACDSY010000000
VERSION     DACDSY010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.314"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1185)
                     /locus_tag="IWK44_003019"
     CDS             complement(<1..1185)
                     /locus_tag="IWK44_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9551459.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFF
                     LGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNS
                     WKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHP
                     DMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKL
                     VQT"
BASE COUNT          495 a          183 c          201 g          387 t
ORIGIN      
        1 tgtttgtact agtttgccat taatagaagc cacttgccct ttataacgat aaataggtgt
       61 cgtcaagaca gtatttttat tattcgaatt atctaattca attgtaagtt tgttactcat
      121 aaaatgagtt gttggattta tttcctggtt acctagtaaa tattgatcat tattaatcat
      181 atcaggatga tagattgact ctttgtttgc ataatcagta tgattgtaat tttcagctat
      241 agcaacagcg tcttcaggtg ctataagtgt taatgaatct ttgttagcat ggatagaatt
      301 gcttactcct gagagatgca atattagtgc aagaaacgta aagaccatta ttttccatga
      361 attattttta gtagaaaaaa tataatagat cactatactc atcgtatagg ctataaataa
      421 agaagaaaaa ccatttaacc gccagacaaa ttgtagactc ttaactggtg tatggttaaa
      481 aagttgccaa ggaaataaat tggttgaaca aaaaagaaca aatacaccaa gaaagaaaat
      541 aaataaatca tcagcagtta atttttttat aaaaataaaa gcaccaatca atccgagaaa
      601 taagaataat ccaatagtat agttgttata gttattattt aaaatatgtg tcagcatatc
      661 actaggagcc ataccactta attcttttcc cagtgggact ttcaattctt gtgctctaga
      721 ttgttgaata aaaggaatta agaatcccgc acttaaaaaa aaggtgagga cggtagcttt
      781 taaaagactt aagagtctag cgattttttg gtcccaaaaa taaaaagaaa gaaacaacgt
      841 tcctccaata aatacagaaa ccatagcgac agaaagtaaa tgagtataca ctactagcgt
      901 catgccaata gacaaccagt accatttttg gtaatctcta atataaattt cgtaacaccc
      961 cattagtatc aaaggcaaaa atgttaaagc gacagcttct cccaatgaag cacgatggaa
     1021 tatgtcaatg gcacgatagg cggaaaatgt ataaataatc gaaaataaca gcgaaatata
     1081 acgattgttt tttatttgtt tcatagaaaa ataagaaacg accattgtta aaaaagtgat
     1141 aaaaaaatag tagatattat aacttaaaac aagatttccc atcattttat aaaataaaaa
     1201 agctggataa agagtaagcc atggataaaa aatattcatc attgttccat gatgatcgaa
     1261 gttatt
//
LOCUS       DACDSY010000305         1260 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.315, whole genome shotgun sequence.
ACCESSION   DACDSY010000305 DACDSY010000000
VERSION     DACDSY010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.315"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>1260
                     /locus_tag="IWK44_003020"
     CDS             <1..>1260
                     /locus_tag="IWK44_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9551460.1"
                     /translation="LNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSK
                     IARLLTEIVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEIN
                     IRNVLFGVYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTIS
                     LLRAYGNQPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLIT
                     EEEFELDGAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMM
                     HVNRKNWIFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRL
                     FYDYLFLLVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSR
                     TNIYLSDCLLEKFNKEQIIWKNPPTPDD"
BASE COUNT          443 a          187 c          226 g          404 t
ORIGIN      
        1 ttaaataaag aatttactga attcaaaaca aagccagaga tccaaatttc agaagaagca
       61 gagatccatg tcgaacgtct gaatttgacg gatatcaaaa atttaagaag aatctacttt
      121 caacgatcaa aaattgctcg actgttaacg gagatcgtgg attcaaaagt aacgatcgaa
      181 aaatttgcag agaatcattt tttaagcata tcaagagcct atgtaaaaag aagagaatta
      241 gtctcttttc ttgaacagca tcaaataaag ttaaagaaaa acatattagt tggggaagaa
      301 ataaacatac ggaacgtatt atttggggta tatttcgggg tctttaatgg tttttctatg
      361 ccatttagag aaagcgtcat gaatgaagca aagagtatcg tgcaatattt tgaatatctg
      421 ttcaatttaa aactgtctgc aacaaaacga atcaaattca atttgctaac cactattagt
      481 ttgttgaggg cctatgggaa tcaaccaatc ggaaaacgtt tcttttcaga tgaatacttt
      541 aagagctcag aaggaatgaa gatcatcaag gatttatcac atatttcttt tcttcatatc
      601 cctaaagagc agatcattga tgagatttct tatatcatgt tgtttttgat cacggaagaa
      661 gaatttgaat tagatggcgc atttggactt gcggaagctc attttcatga tatccacatg
      721 attagtcaaa aaatgtcagt ggaattgatt gatcaattac cattttcgat tgaaatctct
      781 gaagaagaaa gactatatat acgaaaagaa cttcgtatgg gtatgatgca tgtcaatcga
      841 aaaaattgga tattcgactt tgagatgact agctttacgt ctaaaaagca gctacagttt
      901 ttcttcgaga cgtatccaat attcagtaca gcaatccaaa aggtcataca agagaataaa
      961 cgctatttct tgggaaaaga tgaacgtttc atgattcgct tattttatga ttatctcttt
     1021 ttattggtcg gtatttgtcc ggtatcatat tttgaagcac cgattcatgt ctgtattgat
     1081 ttttcgcaag gatctgctta taccgattac attatttctc aagtgaaagg atttaaaaat
     1141 tacaatattg tgattgagag tcgaatcact tctcgtacga atatttattt atctgattgt
     1201 ttacttgaaa aatttaataa agaacagata atttggaaaa atccaccgac tccagatgac
//
LOCUS       DACDSY010000306         1247 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.316, whole genome shotgun sequence.
ACCESSION   DACDSY010000306 DACDSY010000000
VERSION     DACDSY010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.316"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(133..1110)
                     /gene="guaC"
                     /locus_tag="IWK44_003021"
     CDS             complement(133..1110)
                     /gene="guaC"
                     /locus_tag="IWK44_003021"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP9551461.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
BASE COUNT          359 a          269 c          198 g          421 t
ORIGIN      
        1 atgttcaaaa gcttgtatct gcgataataa ttccagccaa acaaaacata taaagaacta
       61 tttttgtttg gctgttctta ttacttaatg caggacaact ttttcacaac atcctaatat
      121 tttatttatt ttttagattg tgtctccatt aaaaatcgaa tttttcacga tgacatagtc
      181 aacttttcga atggcttcca gatctcttcc tccagcataa gaaatagatg attgaaggtc
      241 ttgttgcatt tccactagtg tatctttcaa agatcctttg tgttggatcc agattttctt
      301 tccttcaaca tttcgttttt cgcctttttg gaattctgaa gcagaaccaa agtattcttt
      361 atacacgatt ccattttcga cttttgtttc gccaggagat tcctcgtgac cggcaaacaa
      421 tgaaccaatc atgaccattg tcgcaccaaa acggacagat tttgcaatat cgccatgtgt
      481 tctgatccca ccgtcagcga tgatcggctt gcgagctgct ttcgcacacc atctaagagc
      541 tgccagttgc cagcctccag tcccaaagcc tgtcttgatt tttgtaatac atacttttcc
      601 tggaccaatc ccgacttttg ttgcatctgc gcccgcattt tccaattcac ggacggcttc
      661 cggtgtacca acattaccgg caatcacaaa agtttcaggc aaatattttt taatatgctg
      721 aatcatatca ataactgagt tagcatgtcc atgcgcgatg tcgatagtga tataatcagg
      781 atttagcgat tcttctgcca acttctctat aaaggaatat tcctctttct tgacgccaac
      841 actgatcgag gtaatcaatc cacgcttttt catttttttg ataaacggaa ttcgagctgc
      901 ttcatcaaac cgatgcatga tatagaaata accattttcc gctaagaatt ctgcgattgt
      961 ttcatcaata atcgtctgca tgttggcagg cactactggc attttgaatg tatgtttacc
     1021 taatgtgacc gttgtatcac attcagaacg gctatttacg atacatttat taggaattaa
     1081 ttgaatatct tcgtaatcaa atactttcat tacattatcc tcctgatgtt cacatccagc
     1141 aaaacacgaa cattattaat ttaatcaagg ctttttttag cgccctatgt aatttaactc
     1201 aattaagcaa aaaaatcaag agcaaaaaga gaatttatac gaatggt
//
LOCUS       DACDSY010000307         1245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.317, whole genome shotgun sequence.
ACCESSION   DACDSY010000307 DACDSY010000000
VERSION     DACDSY010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.317"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1244
                     /locus_tag="IWK44_003022"
     CDS             <1..1244
                     /locus_tag="IWK44_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP9551462.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          410 a          222 c          268 g          345 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaa
//
LOCUS       DACDSY010000308         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.318, whole genome shotgun sequence.
ACCESSION   DACDSY010000308 DACDSY010000000
VERSION     DACDSY010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.318"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            193..>1193
                     /locus_tag="IWK44_003023"
     CDS             193..>1193
                     /locus_tag="IWK44_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP9551463.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETF"
BASE COUNT          489 a          173 c          196 g          335 t
ORIGIN      
        1 accctgaaat agagcggttt gctttaattt attttttaag gtaaactaaa aaaagataaa
       61 aaaagactac tgctttaaca aagtttggcg acttatcagc agtagaaaaa tatatagtgg
      121 atagatatat ttgtttaatt cattgtaaca tagttttgaa caaaacgcta tcctacagaa
      181 agaggattgg ctatgaatga ttttaattat tacaaatcaa aagaaattta tcgtgaaaaa
      241 tattatcaaa tgcctaaggt attttttact aacgaaaaat acatggatct ttcaaatgat
      301 gcaaaaattg cttatatgct acttaaagat cgatttgatt attcagtcaa aaataactgg
      361 gtagattctg atgataatat attcttcata ttcacagttg aagaattaat gaaattgtta
      421 caatgccgcg aaggtaaagt atctaagatc aaaaaagaat tagaaaaagc aggtcttctg
      481 aaacagaaga gaggaagagt aaataaacgt gatggtaaga ttgaatctat gcctaattta
      541 ttgtacttag gcaagccaga agttacgaat caagacgtct ttaaaatcat ggaagaggaa
      601 gacaatactg attccaccgt aattgcgaaa atcgcaaata cggcaaaacc gcgaaaaatc
      661 aatgattcta ccgtaattgc gaaaatcgca aatacggcaa aatccagtca tatcaaggac
      721 tcaaccgtaa ttgcgaaaat cgcagacaat ctattctatt ctaattctat agacactaat
      781 agacacataa tagacactga aactgaccag ttacaaaatc aagttttgct agataacttc
      841 gtcgacatca tgcaagagga cagtataaat acatttgtac cagaaaatgt attaaacttg
      901 ataaaaacat tttcttcaag ttacagtgaa gctcaacaaa ccgtccaaac tattcataat
      961 gcaaagaaaa aagctgaaga attagagcaa actattgtcg tttatgaaga actggaatct
     1021 tatggaattg atgctgacaa aggattatat atgactttac tgaaagctta tcaaaaacaa
     1081 aaaactgaaa aagtaaacaa tttgcagaat ctaattttcg tttatgtaaa aaattggttt
     1141 gtcgaaaaag ctattgcggc aaagcttgca aacgaacaaa gagaaacatt taa
//
LOCUS       DACDSY010000309         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.319, whole genome shotgun sequence.
ACCESSION   DACDSY010000309 DACDSY010000000
VERSION     DACDSY010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.319"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IWK44_003024"
     CDS             <1..1076
                     /locus_tag="IWK44_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9551464.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACDSY010000310         1183 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.320, whole genome shotgun sequence.
ACCESSION   DACDSY010000310 DACDSY010000000
VERSION     DACDSY010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.320"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(185..1045)
                     /locus_tag="IWK44_003025"
     CDS             complement(185..1045)
                     /locus_tag="IWK44_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9551465.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT          359 a          225 c          174 g          425 t
ORIGIN      
        1 tgatggttaa cctacttaaa ataaatactg caacgacaaa cggagatcaa gcatgctttg
       61 taacgtattt tacgataatt acgactttat tttattactc tatcttgctg ccatacccgt
      121 ctatccctaa tcatctttgg tgaatttttt caaagttagc ataacaaaaa taagtatttt
      181 ttccttagtt aatcaatagt atcatttttt tcatatgatt taattcatca attaattttt
      241 tgtttttatt aattttagca aaagctagtg catcttgaat caattcgagc acttctgcct
      301 tcgacttatt tttagcctgt gcgttcttcg ctaattgggc agcagctcgt gctaagtaat
      361 aagtcaaatg gttatccgaa caaatatcga ttgcgtaatt tagtagtgca tcacttgttt
      421 ccaaatcacc gtcttgcgca aaaaaaacac cacaatgaaa gacgatattt aacacccgcc
      481 agatatcttc tgttgtacga atcggatacg tataaatttt ttcaaacact cgattgaaat
      541 aaacttctgc tttttccaac tctttttctc tagcatagac cattccaata cctgtatatg
      601 ccaataaacg aaacaacgtg ttggctgttt ctgccatcaa caactgatca aaatggaaca
      661 acacatccgt aattggtgca ttcgtaaaaa tcatttcaaa tccttttaga tagtagtaac
      721 gtgcaatcag ctcagactcg tcaattacct tgggatcaat ggctgctaaa atctgtgtca
      781 attgttcata ttcactcgta atcagtgaaa actctgcttt ttctagctgt tcatcaagtt
      841 gtgtattgat cacgttcatc gaagaaaaaa gttcaactaa cggaagattg attcgattac
      901 ataatttgat taaaattttt aacgaaggga cttgaccatt gttctcaaat ttacttaatg
      961 ttgcttgtgt acaaattcct tcagacactt ctttttgaga taagttggca gcctttcttg
     1021 ccgtaataaa tttttcaatg ttcattattt tctctccaat cagacctcac acgaaaatca
     1081 tttctgtctc gattttagca tgttttcttt gggaatgctt taggttctgt tgcaaagttt
     1141 taaataaaga ataaagtccc ttacggtatc tatgatttaa gct
//
LOCUS       DACDSY010000311         1176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.321, whole genome shotgun sequence.
ACCESSION   DACDSY010000311 DACDSY010000000
VERSION     DACDSY010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.321"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..626)
                     /locus_tag="IWK44_003026"
     CDS             complement(<1..626)
                     /locus_tag="IWK44_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9551466.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
     gene            914..>1176
                     /locus_tag="IWK44_003027"
     CDS             914..>1176
                     /locus_tag="IWK44_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9551467.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDA"
BASE COUNT          387 a          190 c          225 g          374 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccatagag cgactccttt ggtagggtga tgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caacaacaca aaatattata gaacccttta ttctaaagga
      781 tttcaaggtg tctgtttgca tttattagaa aaaagaaaaa gtattggcaa cagaaggtgt
      841 caccaacact agatattata gagccataat atgctgtata atatctagtg tataggctta
      901 agtggaggta tatatgaaaa tattaactgt tgaagatgat aatatgataa gagagggaat
      961 aagtgaatat ctttcagaat ttggatatac tgttattcaa gctaaagatg gaagagaagc
     1021 cttgtcaaaa tttaatagcg atataaattt ggttatctta gacattcaga taccttttat
     1081 aaatggttta gaagtgttga aagaaattag aaagaaaagc aatttaccaa ttctaatctt
     1141 gactgcattt agtgatgaag aatataaaat tgatgc
//
LOCUS       DACDSY010000312         1148 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.323, whole genome shotgun sequence.
ACCESSION   DACDSY010000312 DACDSY010000000
VERSION     DACDSY010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.323"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            27..437
                     /locus_tag="IWK44_003028"
     CDS             27..437
                     /locus_tag="IWK44_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9551468.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            451..807
                     /locus_tag="IWK44_003029"
     CDS             451..807
                     /locus_tag="IWK44_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP9551469.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          404 a          170 c          218 g          356 t
ORIGIN      
        1 gattataatt aaaaatgagt gagaaaatga taaaagtata tacttcgcct agctgtactt
       61 cctgtagaaa agcaagagca tggttgcaag cgaatcaact tgaatttgaa gagaagaata
      121 tttttgccga accgttaaca gaaagtgaga tcaaaaagat cttggcagcg actgaaggag
      181 gagttcaaga tattatttcc actcggtcga aaatttacga aaagctgggc atagatttta
      241 acgaattaac tttaaagcaa atgatcgtct tattcaaaga atatccttca ttattgagac
      301 gacctatttt gattgatgaa aaaagaatac taatcggatt taatgaagat gagatccggt
      361 catttgtccc tcgtgaaatt cgatgtgttg ccagaaatct gatctattta caaaacaata
      421 acatcttaaa ttcataggga gggagccttc gtggaagtga aagtctatgg atcagcgcac
      481 tgtgtaacaa gtctgaaggc gaaacaatgg ttagaacacc atcaattgcc ttatcaattt
      541 gttgacttag aagaacgcga tctaagtaca gaagaagcag aagaattatg cagttttgaa
      601 gaagtgcaag cagaacagct atttgcgact tggtctgagg cttttcacaa cattgcgatt
      661 gacctttcct gtgtaggaaa aaaacaactg atttttttat gcacacaaca taccaatctc
      721 ctcagacgtc ctttgattat tatcgatgaa gttctattta ttggatacaa tgagcagttg
      781 atggaacagc ttatcttaaa taaatagact ttaactgttt taatcaagtc actgagcatt
      841 tcatacagtt gtaacagtcg aacacttagc atcaagcgtg ttatgtgttc tttttttgac
      901 tttatttttc tactatagag acatgctcaa tttatgtggt caaaattcaa atctaggtgg
      961 ggatatagag ttaaaaatat attgacatgt gtaaatttac atgatatatt catttctgtt
     1021 gttttaagta gcaaagcaat aaaatagtat tttaaaaaac atataaaata gttgttgaca
     1081 tgttagaaat gacatgataa gataactgag ttgttaaaaa agaaccactt gcgaagcttg
     1141 attctatc
//
LOCUS       DACDSY010000313         1129 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.324, whole genome shotgun sequence.
ACCESSION   DACDSY010000313 DACDSY010000000
VERSION     DACDSY010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.324"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWK44_003030"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWK44_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP9551470.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          335 a          229 c          194 g          371 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagcc
//
LOCUS       DACDSY010000314         1114 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.326, whole genome shotgun sequence.
ACCESSION   DACDSY010000314 DACDSY010000000
VERSION     DACDSY010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.326"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..897)
                     /gene="vanY"
                     /locus_tag="IWK44_003031"
     CDS             complement(<1..897)
                     /gene="vanY"
                     /locus_tag="IWK44_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9551471.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          399 t
ORIGIN      
        1 agtatgtgtt gatccgggaa acactgtcac aattacaccg tctatattgt ttcctgatat
       61 ctcataacga agattagtcg gcacatgaat ggtggtattt ttagtaacag gataataaaa
      121 gatctcatat ttttccccat ttacactaac agaaatggtt ttttcttctt ttaggtaatc
      181 catatattcc tcgagaacga aattcttttc tttcataatc gcactatgtg gtaaaccaac
      241 atagcgaata tgccatggtt catattgaat tcctgttaac tctgttttgt cctctggata
      301 acgtaaaatg aacccgtatt tccaagcatt ttcttctatc cactttcctt caggggctcg
      361 ttccattttc gtcaagcttg atcctacatc tagtgataaa cctgaattat gctcactata
      421 acctgctggt aaggcatact cagcccccat ttcttggtaa agcacacttt gctcatcaaa
      481 gtctcgatag ccactattaa taataaaatg actaacgcca ccctttacag catcattgac
      541 catctctgaa aatttttgtg ctatttcttt tgacatataa atattactat caagcaaccc
      601 gtatccattt attaattcgt catgtttaga taaattcacg atatctgact tcacactttc
      661 ttggcgaaca ggatatttac tattgattaa tagcagattt ccttgataaa cctgttcttc
      721 tgtaattgtt ttctcttggg tattttcaga agtcccacta ttttctaaat gttctttggg
      781 attttgatca taattttgaa attcgacttt ttcctgagaa aacagtgctt cattaacgta
      841 gtcataacct aagtatatta agaataacaa taacaataaa aaaaacaact tcttcatttt
      901 cagtctcctc cttaactata tagttggaag tatgaaatat ttctagaatc ttccccttaa
      961 acggcacaat ctcgtttgag attgtgccgt tttgccccgt aaattacagt caattatttc
     1021 gccctactgg atttactatt atcaccaatg tagatgaatt ttcataagtg atacccatgc
     1081 tgaatttaac gaggctgaac tgcctgttga caat
//
LOCUS       DACDSY010000315         1107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.327, whole genome shotgun sequence.
ACCESSION   DACDSY010000315 DACDSY010000000
VERSION     DACDSY010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.327"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(315..800)
                     /locus_tag="IWK44_003032"
     CDS             complement(315..800)
                     /locus_tag="IWK44_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551472.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(939..>1107)
                     /locus_tag="IWK44_003033"
     CDS             complement(939..>1107)
                     /locus_tag="IWK44_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP9551473.1"
                     /translation="LNWLLVGLFELDLVAMIAGGSTTIFAKIIYIVVGICAIYCLKFF
                     PMITRKVDERY"
BASE COUNT          413 a          184 c          184 g          326 t
ORIGIN      
        1 aagggatcat taaaataata agaagaaaat atgtcaaaag gtaaaaaagt aaattcttct
       61 atccaaatat agaaaaattg aatctagaaa accgcccgac aataaatttc attaactcag
      121 ttgaccgaaa atgaagtaga taccaaaaat ttatcaagag agcaagtaga gaagtgagta
      181 tattcataag agaaatagct gtaacgaatt attaggttaa tgcaaatgct tatttatact
      241 ccgtataact ttctcagaaa aaatgatacc aataatgact tgctagtact gtaaatccgt
      301 ataatatcat ctaatcaaaa tggtgttcca tttgcatctg tcatcgtaat cgatccttcg
      361 ggtgacactc gataaaaacc aacgcttcca gatcctcctt gggaacggat ttgtttgctg
      421 gaagctgtga acaaataatc tcgatcaatt ttttgataaa agctaataac aatatcgtca
      481 ctttgtaatt cggaacggtg ggcaataaga aatagaagtg ctttgtcaga gttttctaat
      541 ggaagagaag gtacagtgct ttcttgcatt tcagatgttg aagaaatgct aaacgactga
      601 atacttgatt ggctatgttg cgaatctaca ttaccagtag aagtattagt aatggaagta
      661 gtagaagtac ttttagttga accaacctca gttgttgaaa tggctgctga agattcttta
      721 acagaattct ttgttttata tccacaggca gaaaaaagta ttacagttcc caacaagcct
      781 gcgtaaatcc ctttatgcat gatagctcct cctaaaatag attatcttta ttcagtatat
      841 cacttaaaca aagaaaagca gatggtttaa aatgaaaaaa ccttcccaat aaagggaagg
      901 ttcatgaaga taataacttt tttaataatt atagctttct aatatctttc atcaactttt
      961 ctagtaatca ttgggaaaaa tttcaagcag tagatcgcac aaatacctac gacgatataa
     1021 atgatcttcg caaaaatagt tgttgaacca ccagcaatca tagctactaa atcaagttcg
     1081 aacaagccta ccaacagcca gttcaac
//
LOCUS       DACDSY010000316         1100 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.328, whole genome shotgun sequence.
ACCESSION   DACDSY010000316 DACDSY010000000
VERSION     DACDSY010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.328"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..285
                     /locus_tag="IWK44_003034"
     CDS             <1..285
                     /locus_tag="IWK44_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9551474.1"
                     /translation="TFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQLD
                     LELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
     gene            complement(269..862)
                     /locus_tag="IWK44_003035"
                     /pseudo
     CDS             complement(269..862)
                     /locus_tag="IWK44_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT          329 a          221 c          194 g          356 t
ORIGIN      
        1 acttttgaca tcaatcgatc attgagtcat aaaggctgtc cttttgataa tgccgtagct
       61 gaatcaactt ataagtcgtt gaaagtagaa tttgtctatc aatacacatt tgaaacctta
      121 caacaattgg atttggagtt atttgactat gtaaattggt ggaaccacct tcggttgcac
      181 ggtacacttg gctacgagac accggttggt taccgtaacc agagattggc gcagcgaatc
      241 cttgataatg agctcggatg tgctaacgct agcgaggcag tctaacttta actgttagct
      301 cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa ggacaatcgg aatagcatac
      361 ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac tgttgccatt ccaccagaca
      421 cttgcttaaa gcgaaaaatt tcttcaatgt ccatgcctaa ttctttggat attctcttgt
      481 ctgaccatcc attttttact aatctttgga tgacaatttc cattcctctt atttcatggg
      541 tccctcttgc acgattgtgt cgaattgttg aagccatacg ttcttatatg ggtttatcaa
      601 taactgttac cggtacaact tttaagttga gtctttcctt agcaacacga tacctatgaa
      661 gcacatcaac caagatatac ttctcttctt tttccttgta ataacaaact aatggttgag
      721 taaagccatc ttcaatgata gaaagctcca ataagttcat ttccggagac gctactcgat
      781 tgggattata atcgttcgcc tgtattttgg taattggaac aagccgaacg tccatcacag
      841 ggaaagttat gtctttattc atacgcttgt ataccctact ttcttaaaaa gtagggtata
      901 ctcaagaatc cgattaatta tcttctcaat ttggataata tatctcatca gtcttcttgt
      961 cactttatca acatgatttt gctgcaaaat gggataccga cagtactgga ctggtaatca
     1021 ggataaaagc aaaaataatt aatttgtatc ctaaaaaatc cataaaaatt ctctcacatt
     1081 ttcgggtatt aattcgagaa
//
LOCUS       DACDSY010000317         1074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.329, whole genome shotgun sequence.
ACCESSION   DACDSY010000317 DACDSY010000000
VERSION     DACDSY010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.329"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..276)
                     /locus_tag="IWK44_003036"
     CDS             complement(<1..276)
                     /locus_tag="IWK44_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9551475.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
     gene            complement(775..918)
                     /locus_tag="IWK44_003037"
     CDS             complement(775..918)
                     /locus_tag="IWK44_003037"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551476.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            838..>1074
                     /locus_tag="IWK44_003038"
     rRNA            838..>1074
                     /locus_tag="IWK44_003038"
                     /product="16S ribosomal RNA"
BASE COUNT          351 a          194 c          205 g          324 t
ORIGIN      
        1 gacgtcaggc gtccaaccaa gagtgacttt ctctttaatg taatctactt catgagcagg
       61 taattgaatg acttttctac cacatttttt cttattttct ttatactggt gccaataatc
      121 aagagcagtc ttgccttgct tgaacttatt gactacatta taaatagttt gtatagcacg
      181 tcccattcgg ttagcgattt caaccggctt attatgttga agataatatg attctatcat
      241 tgtcagttcg tctatggtaa gatgtttgta ggtcatttat ggttacactc ctttgttttc
      301 tttcgtcgga aatacaattt gagtgtacca taaatgcctt tttatttttc taacttaatt
      361 ttacaattcg cgatatatta aacaacgttg tcgcgcttca ttgaaacatt ttatgaaaat
      421 tccaaaaaag agttgacaca tgtttttaga cgtgataaga taactaagtc gttaaaaaga
      481 accacttgcg aagcttgatt ctatcgaaaa aaaacttcaa aaaagttctt gacaaataac
      541 aaacgataag ttatgataaa tgagttgctg aaagatttcg aaaaacgaaa tcgaaaaaaa
      601 cttttaaaaa agagttgaca atcgcttgtc tctctgatat gatataaaag ttgctacggc
      661 agccaaaaac gaatcgggaa acactcccga tgaagtagac ctttgaaaac tgaacaaagt
      721 aagacaaacc aaatgtgtag ggcgtcttga ttcaattcaa gacaataaac atttttaaca
      781 agcaagcaat atgctagcaa acaaattgag cttaacaatc gcaagattgt tcgaactttt
      841 tatgagagtt tgatcctggc tcaggacgaa cgctggcggc gtgcctaata catgcaagtc
      901 gaacgcttct ttttccaccg gagcttgctc caccggaaaa agaggagtgg cgaacgggtg
      961 agtaacacgt gggtaacctg cccatcagaa ggggataaca cttggaaaca ggtgctaata
     1021 ccgtataaca atcgaaaccg catggttttg atttgaaagg cgctttcggg tgtc
//
LOCUS       DACDSY010000318         1060 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.330, whole genome shotgun sequence.
ACCESSION   DACDSY010000318 DACDSY010000000
VERSION     DACDSY010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.330"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            73..690
                     /locus_tag="IWK44_003039"
     CDS             73..690
                     /locus_tag="IWK44_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551477.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          383 a          141 c          180 g          356 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccgagaa ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt
      121 ttaaagtgga aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt
      181 atttttactg tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa
      241 ggtcgtttag ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc
      301 ctatatgact atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa
      361 gtaaaagttg gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc
      421 tatacagtag aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga
      481 acagttgtga aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct
      541 attaaaggta cggggaaaag aactatagtt gaggtttcat ctacgtatcc aactacgatg
      601 attctatctt tattaattag ttcaataact tttatattct tatttacacg ttatttaata
      661 agatgctgcg gtgaaatgaa aatagattag atagatatta taaatatgtt gattctattt
      721 tcatagcaat ggagaaaata cattagcaat atataaaaga aggtatcaag agtcacagtg
      781 aatttattcc gaaaagtgaa aagagcaaat ataaaattta atgaaaatat agaactaaat
      841 aggaaaacgt agacgtaatt ttctacgttt tccttttcat atatattaaa tattacggaa
      901 aattatataa actaatttag tgttttgttt gtaattccta attttgctcc acttttagat
      961 aatttttagg tctatttaac tcatcagaca ttctatgctc atttttctcc aaatttgttt
     1021 caactctttc gtacttaatt ttgcgtattt gaaaattctt
//
LOCUS       DACDSY010000319         1059 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.331, whole genome shotgun sequence.
ACCESSION   DACDSY010000319 DACDSY010000000
VERSION     DACDSY010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.331"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(37..993)
                     /locus_tag="IWK44_003040"
     CDS             complement(37..993)
                     /locus_tag="IWK44_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551478.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgt
//
LOCUS       DACDSY010000320         1052 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.332, whole genome shotgun sequence.
ACCESSION   DACDSY010000320 DACDSY010000000
VERSION     DACDSY010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.332"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            29..814
                     /locus_tag="IWK44_003041"
     CDS             29..814
                     /locus_tag="IWK44_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP9551479.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT          405 a          158 c          174 g          315 t
ORIGIN      
        1 attcttgata acaagctacg tgacgtatat gcctaacaaa aatagaactt ttgaatcaat
       61 cttaaatgaa ggtttaacca cagaaccggc atccattatt tttgatgaag ttatcgatga
      121 cgttgaagat agtaagtatc ataaaaattt aaaagaactt ttttattttg cactcaacaa
      181 tggaccaagt ttgttcactg gtatatcagt acctgatagt gataatccat taattatggc
      241 aaaaatttac ttaacaaaat ggtgtaataa atacatcagg gataggaata acccagcatt
      301 aaaaaaacct cttaaaacat ttggcgaaaa agatgctgct ttaactacta gagtagcagc
      361 aaatgctaat attgatgatc aaaaagtatt gaatgactac ttgcgtggac attttttata
      421 tatgagcgct gagaacatga acggaagtat tttagaggaa tatttagccg aagtgttaga
      481 acctgaaggt tggatttggt gcgccggctc tgtatatcga gctgttgatt tctgttattt
      541 aggaacctca ccaattcttt tacaagtgaa aaataaatac aatacagaaa gcagttcgag
      601 ttcagctata agagtaggaa caacaattag aaaatggaat cgtttaaata agtcgacaaa
      661 aatttctggt ttggatagcc ctatccccaa ttggaaagca ttaatagaaa tgactgaagc
      721 tagtaaggaa ctggcagcta agttaactga aaatagttat ttagcttata ttaatgaaaa
      781 atccacacga gaattatgga cactcgatga ctaacaatat ccttattagt atctatccac
      841 gatacatgga aaaaattata aacaaacaaa aaaactttga atttagaaat ttctatctta
      901 cctcagatcc ttataatatc ttttggattt acgaaacaat ccccaccaaa gctttaaagt
      961 ataaattgat tgtaaaaaat ccaattacta aacttaagaa aaatcaacac tactttttag
     1021 gagacgatga gtttgaacaa ttagtttaaa ag
//
LOCUS       DACDSY010000321         1051 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.333, whole genome shotgun sequence.
ACCESSION   DACDSY010000321 DACDSY010000000
VERSION     DACDSY010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.333"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..980
                     /locus_tag="IWK44_003042"
     CDS             6..980
                     /locus_tag="IWK44_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9551480.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DACDSY010000322         1032 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.334, whole genome shotgun sequence.
ACCESSION   DACDSY010000322 DACDSY010000000
VERSION     DACDSY010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.334"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..487)
                     /locus_tag="IWK44_003043"
     CDS             complement(<1..487)
                     /locus_tag="IWK44_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9551481.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VL"
     gene            complement(597..>1032)
                     /locus_tag="IWK44_003044"
     CDS             complement(597..>1032)
                     /locus_tag="IWK44_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP9551482.1"
                     /translation="SGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSI
                     SMAILADIILSVFGYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSA
                     ATMDAMIHYAVGIWGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT          377 a          201 c          145 g          309 t
ORIGIN      
        1 ttagaaccaa tccaatttta aagttaatga aaaagcctaa ggcaactgat aaaacaggtg
       61 ttactatggc ttgagcacta tctggaatca agtgagcaat tgccatatgt gttttagccg
      121 cgttgtcatc aattgtcttt cgaatatatc ccgaagaatt taaatcaaaa aaacgaaaac
      181 ttgcttctgt taaaccgcta attccctttt ttctcaaatt agtctctaag cgaaacccaa
      241 ggatatgaga aaataagcca gaaaaaatat aaaataaagc tcctaaagtt aaccaagcaa
      301 cagtttgaac cgcataactc ttagctaaat attcatctcc tacaataatt agagactgaa
      361 gaaatttgta aatactgtaa tatccataga ctgttaatac gacagaaaaa tcagaaaata
      421 aagtggctaa ccatccgtag tatttcattt tagggacata actaaataat tttttgtata
      481 ctttcatttt ttcctccccc gcattcaatt tcttctctta ttcgtttaca actgttaatg
      541 ctttccgtaa tagcgtaaat tagagatgta caaacagata aaaggtaaac tacgttctat
      601 tttttgtatt tttttaagac ttttcttcca atcaatacac caatcataga taaacttgct
      661 acaactaaca ctgaaatgat tccccatata cctaccgcat agtggatcat tgcatccatt
      721 gttgcagcag attgtccttt atctatccaa tcttgacgaa aacgatctgt aaagaaccat
      781 ataggaatcc actgtccggc agcactgcct aattgcatta gagcccaaga cgcaacttga
      841 ggaattgctc gatcatatcc aaatacagat agaataatat cagctaagat cgccatacta
      901 atcgaactga taaaccaagg taagtacgcc ccgcccatta acgtaaaaaa taacaataaa
      961 acaattgtgt aaatagtaaa aattcctttt ctagggtagt tatagctaat ataaacaaat
     1021 atgattccag at
//
LOCUS       DACDSY010000323          995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.336, whole genome shotgun sequence.
ACCESSION   DACDSY010000323 DACDSY010000000
VERSION     DACDSY010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.336"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            362..995
                     /locus_tag="IWK44_003045"
                     /pseudo
     CDS             362..995
                     /locus_tag="IWK44_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          339 a          181 c          183 g          292 t
ORIGIN      
        1 gttagtagag aaaatgtagt tatatattta aatttttacc agaaagaaat gaacaccttc
       61 aattatctaa atctcgaaaa tggttagatg taatcatcag gaaaaacaat cttaaacgaa
      121 ttactattca tggtcttaga cacacacatg ctagcttgct tttagcaagc tagtgcaaat
      181 attaaagatg tgcaagagcg tttaggccac tcttctattc aaatcattat ggatctatat
      241 attcatatta cagacaaaag aaaagaagaa accgtagcgc aattcgcaaa atataggctc
      301 tataatttat gggactgatg aatcaaaatt gattcttcag tcccattgtt attttaggta
      361 tatgattcgt acattgaaag agttgaaaaa ctatcttccg aagccgtcaa aaaggaccct
      421 gccaaaagta ttgatggtgg atgaatttcg ttcgcacgct tcaattgtgg ataaaatgag
      481 ctttatttgt gcggatggcg agactggaaa actcattgat attttgccta cacgaaaatt
      541 gtctcgatta acacactatt ttagtcgctg tgcaaatcct gaagcggtgg aatatttagt
      601 gacagatatg aacgccgcct acttccaact gacaaaacgt gtcttaccaa atgcgaaagt
      661 cgtcattgat tgttttcata tcatcaaaca tttgaatcag gcgttcaatg attttcgtgt
      721 acaagaaatg aaaagattac accagttagg gcaacgacgg gaagccgaga aagtgaaaaa
      781 gaactggcgc tttcttctaa aaaaccgtca aaaaatcaat cactatgatt acaaaacatg
      841 gcgcagtttt cgtgcaccta aatacccgct actcactgaa gcaatgatga ttgatcgatt
      901 gctggcattt tcaacgcctt taaaggacgc ctatactttt ttccatgagc tgaccgaagc
      961 ctttagagac aaggaatcag acttattttt tacct
//
LOCUS       DACDSY010000324          995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.337, whole genome shotgun sequence.
ACCESSION   DACDSY010000324 DACDSY010000000
VERSION     DACDSY010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.337"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(26..913)
                     /locus_tag="IWK44_003046"
     CDS             complement(26..913)
                     /locus_tag="IWK44_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9551483.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          321 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaa
//
LOCUS       DACDSY010000325          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.338, whole genome shotgun sequence.
ACCESSION   DACDSY010000325 DACDSY010000000
VERSION     DACDSY010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.338"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>994)
                     /locus_tag="IWK44_003047"
     rRNA            complement(<1..>994)
                     /locus_tag="IWK44_003047"
                     /product="16S ribosomal RNA"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DACDSY010000326          984 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.339, whole genome shotgun sequence.
ACCESSION   DACDSY010000326 DACDSY010000000
VERSION     DACDSY010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.339"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..704
                     /locus_tag="IWK44_003048"
     CDS             <1..704
                     /locus_tag="IWK44_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9551484.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          332 a          161 c          163 g          328 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aata
//
LOCUS       DACDSY010000327          965 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.340, whole genome shotgun sequence.
ACCESSION   DACDSY010000327 DACDSY010000000
VERSION     DACDSY010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.340"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..328
                     /locus_tag="IWK44_003049"
                     /pseudo
     CDS             1..328
                     /locus_tag="IWK44_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
     gene            362..>965
                     /locus_tag="IWK44_003050"
     CDS             362..>965
                     /locus_tag="IWK44_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HAP9551485.1"
                     /translation="MPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKIE
                     SNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLSQKKNV
                     IVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTICVLSTT
                     EFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNEL"
BASE COUNT          353 a          107 c          181 g          324 t
ORIGIN      
        1 aattattgat tcgacaggtc tatgtttatt agatgaattc ttggcggata tcagtctatt
       61 tataatgttt attgatgtaa ttgaagaaac atcaagaaat tttttgctgt ctaagttcag
      121 aaaatttcgt atgaaaaatg ataaacaaaa attattggca aatgagattt ggagtagttt
      181 agaagtcgaa gaaagtaaac aagaactttt gttgaaatgg atactttttc ttgtaaagaa
      241 gttctatgtg aaaataccag aaacccattg tctgatagtt atgcacggta aaataacagc
      301 ttctgcgatc gcaagggaaa caaataagtt gctgaataca tatgtttatg aggctttcga
      361 tatgccaatt gaaggtgaga ctagtgactt aattaaccta atcaacaatt tttgtaaaag
      421 tatagatacg acgaatggac tcattctgct agttgatatg ggatcattgg aacaaatgta
      481 tgaaaaaatt gaatccaatg taataggtga tttggtaatt ttaaacaatg tttctacagc
      541 tttagcgatt gaatgtggga ttcaagtttg ccaaaaaaag ccaattagtc atttttacca
      601 gatggatttt gattcgtttc aagttaaagt tcagtattat aaaggattat cacaaaagaa
      661 aaatgtaata gtatcttgtc tctctggtga ggggatttct gaaaaagtta aggatatttt
      721 aaaacggtat ctcgataatc aagtggagat tttgattttt gattttaacg cattaaagaa
      781 aatagcaaag gaaaaagata ctatgatttt taagaataca atttgtgttc tttcaaccac
      841 tgaattttac attcaaggaa tcgattgttt aaatttagaa aatctaatta atgggaatca
      901 aaccttagaa aaattaaata agtatatgga ctcggatagc tgtgagttct gcttaaatga
      961 attag
//
LOCUS       DACDSY010000328          962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.341, whole genome shotgun sequence.
ACCESSION   DACDSY010000328 DACDSY010000000
VERSION     DACDSY010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.341"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..443)
                     /locus_tag="IWK44_003051"
     CDS             complement(<1..443)
                     /locus_tag="IWK44_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9551486.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNIS"
     gene            513..863
                     /locus_tag="IWK44_003052"
     CDS             513..863
                     /locus_tag="IWK44_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9551487.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
BASE COUNT          287 a          144 c          181 g          350 t
ORIGIN      
        1 tcagaaatat ttcttttgaa tgcctgtttt ccacgttttt ccttataacc atttggtttt
       61 cgttttcctt gcatcggtaa atgtaaaaca ttgaattctc ccgttttaaa ccggcgatag
      121 agggtactta ctgaacaaga gaaggttcgc tctgcacggc caataatgac gtctggggtc
      181 caaccttcag ttgatctttt tgcaatgtat tctttttcct cagcaggaaa aatgatttcg
      241 gttctcccac aacgtcgctt attttctttg tattgttcaa agtattcaag tgctgttcca
      301 ccacatttga gaaagttata gacattgtaa attgtttggc gtccacgttt aagctgcttc
      361 gcaacgatgg caaccggagt ttcttgatga aaatatgctt ctatcattac aagctcgttt
      421 ggtgtaagat gggtataagt catttatgtt cactctcctt gtatgcttta gcgggtatta
      481 caatttgagt gtaacataaa tggctttttt atttgtctcg cttaattata caaacggcgt
      541 tttataaagc aatcattcct gagaacttag atgaggttaa actttattta tgggaagata
      601 acggagaaat tgtcggtttt agtggtgtaa gtgcagaaga attagttatg ctatttcttg
      661 atccagattt tattggtaag cgatatggta gtcaaatatt aattgagctg attgaaactg
      721 aagatataaa aagaattgat gttaatagtc aaaataaata tgcaaaacat ttttatttaa
      781 gccatggatt tgaaattgaa tctgaagaag acttagatgg ttttggtaaa ccatacccta
      841 taacgcattt agtaaaaaaa taattcatga atggtttatt acctgattga acatgaataa
      901 aagtaatatt tttattttga tggcctattt ctaggttatg aattatccag atcaaatttg
      961 tg
//
LOCUS       DACDSY010000329          950 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.342, whole genome shotgun sequence.
ACCESSION   DACDSY010000329 DACDSY010000000
VERSION     DACDSY010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.342"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            59..841
                     /locus_tag="IWK44_003053"
     CDS             59..841
                     /locus_tag="IWK44_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP9551488.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
BASE COUNT          333 a          135 c          196 g          286 t
ORIGIN      
        1 agtaaattga gtatcaatat aaactttata tgaacataat cagttacgag gtgaaatcat
       61 gagcaatttg attaacggaa aaataccaaa tcaagcgatt caaacattaa aaatcgtaaa
      121 agatttattt ggaagttcaa tagttggagt atatctattt ggttcagcag taaatggtgg
      181 tttacgcatt aacagcgatg tagatgttct agtcgtcgtg aatcatagtt tacctcaatt
      241 aactcgaaaa aaactaacag aaagactaat gactatatca ggaaagattg gaaatacgga
      301 ttctgttaga ccacttgaag ttacggttat aaataggagt gaagttgtcc cttggcaata
      361 tcctccaaaa agagaattta tatacggtga gtggctcagg ggtgaatttg agaatggaca
      421 aattcaggaa ccaagctatg atcctgattt ggctattgtt ttagcacaag caagaaagaa
      481 tagtatttct ctatttggtc ctgattcttc aagtatactt gtctccgtac ctttgacaga
      541 tattcgaaga gcaattaagg attctttgcc agaactaatt gaggggataa aaggtgatga
      601 gcgtaatgta attttaaccc tagctcgaat gtggcaaaca gtgactactg gtgaaattac
      661 ctcgaaagat gtcgctgcag aatgggctat acctctttta cctaaagagc atgtaacttt
      721 actggatata gctagaaaag gctatcgggg agagtgtgat gataagtggg aaggactata
      781 ttcaaaggtg aaagcactcg ttaagtatat gaaaaattct atagaaactt ctctcaatta
      841 ggctaatttt attgcaataa caggtgctta cttttaaaac tactgattta ttgataaata
      901 ttgaacaatt tttgggaaga ataaagcgtc ctcttgtgaa attagagaac
//
LOCUS       DACDSY010000330          924 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.343, whole genome shotgun sequence.
ACCESSION   DACDSY010000330 DACDSY010000000
VERSION     DACDSY010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.343"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(118..>924)
                     /locus_tag="IWK44_003054"
     CDS             complement(118..>924)
                     /locus_tag="IWK44_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAP9551489.1"
                     /translation="TYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGL
                     QLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDL
                     TYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDR
                     GKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLR
                     LELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          246 a          213 c          177 g          288 t
ORIGIN      
        1 actttttttg acaaaaatta taaggtgttg tgaatgctgc ggtgttatgg ctacgattgc
       61 ccttgacaat gagcatcctc ggatctgatt ttctccggca gaagcacaat cattcaatta
      121 cgctgcctct ccagaggtat cagatccgcg ctcattatca aggattcgct tcgccaatct
      181 ctgttgtcga atttggatcg gtgtttcata cgccaacgtg ccatgtaagc gtagatagtt
      241 ccaccaatgc acataatcaa acaattctag acgtagctgt gccagtgtct caaattggta
      301 ttgatgcacg aattctactt tgacagattt ataggttgat tccacaacgg cattatcata
      361 cggacaaccc ttcctactca atgaacgagt aattccaaaa ccattcagaa tctcatgaat
      421 ggtttggtta tcaaattcct ttccccggtc tgtatgaaaa agcttgacgt ctgttaaaga
      481 atacggtatc cgtccaaagg cttcttttac caatgaggca tctttcttct caccacagga
      541 ataaccaata atttctcgat taaacaaatc aagtattaag cagatataat gccacttttt
      601 ccccacgcga acataagtaa gatccgtaac gatggcttcc aatggctgtt cttgtgtaaa
      661 ggtccgatct aatacattcg ctgttttcgc ttcattacaa gctgttcgtt gccctttaaa
      721 atgagcgatc gtataggtag atgtcaatcc gcggcgtttc atgattcgac cgattcggcg
      781 ccgactgagt tgaagcccac gctttgctaa acacttcttc aattttcggg taccgtaagc
      841 ctttcggttt cgaataaact cttcctgaac gatctcttct aagtcggact cattttcgat
      901 cggtttcgct tgataatagt aggt
//
LOCUS       DACDSY010000331          920 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.344, whole genome shotgun sequence.
ACCESSION   DACDSY010000331 DACDSY010000000
VERSION     DACDSY010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.344"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            28..>920
                     /locus_tag="IWK44_003055"
     CDS             28..>920
                     /locus_tag="IWK44_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9551490.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNET"
BASE COUNT          371 a          203 c          172 g          174 t
ORIGIN      
        1 acaaaaaaat caaggagtga gagagttatg agatattcaa ccatcttaat atcaacattt
       61 gcagccttaa gcttatcgct tactataaat cagacagtcc acacagagga gatagaaaca
      121 gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca ggatgaattt
      181 tcatccttca attcaggagt cgcaaaatta agaaaaggac aaacagcgac catcaaagca
      241 aaaattgact attcaggaga cttagcacct atactacaat cagctgttac atttgagaat
      301 gttgatcccg ctttgtcgat tggctatgac gagaacactt tgactatcca acacaagcaa
      361 gcgatattta ccttcaccgt tacattaaat caggacttgt taaaaccagc gctttttact
      421 gtcaaggtat cagatggact accgaacgat catcatcatt taacgccgta tagtcagcgg
      481 cagacaattg agcaagattc agctatcgac tcaggaggag atcttgttga agaaccaaca
      541 gataaaccag aaaatgaaaa taagccagaa gtaccgccta cagaaaatcc agacggagaa
      601 cagaaaccgg aaatagaacc tggagaagaa cccgatacag aaactcaacc agagccagac
      661 aatgaatcaa aacctgaaat aacccctgga gaaaaaccag atgtagaccc agaagaaaaa
      721 ccagatgtga ctccagaacc agatacggat tctggaaacc aaacagtacc ggaaacaaat
      781 ccagacacag ataatgaaac tgaaaatcca gaaaaaccag aggtagcccc agaagaaaaa
      841 ccagatgtaa ctccagaacc agatacggat tctggaaacc aaacagtacc ggaaacaaat
      901 ccagacacag ataatgaaac
//
LOCUS       DACDSY010000332          918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.345, whole genome shotgun sequence.
ACCESSION   DACDSY010000332 DACDSY010000000
VERSION     DACDSY010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.345"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          306 a          147 c          110 g          355 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatt
//
LOCUS       DACDSY010000333          917 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.346, whole genome shotgun sequence.
ACCESSION   DACDSY010000333 DACDSY010000000
VERSION     DACDSY010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.346"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>917
                     /locus_tag="IWK44_003056"
     CDS             <1..>917
                     /locus_tag="IWK44_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9551491.1"
                     /translation="SLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLLIGNIYFL
                     FSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTFLNIAISV
                     ALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYALKISLPLI
                     PHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWVPWFYGEM
                     SKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVPITLGYFF
                     QFLYSLPVNAEFYEKKTSFIAIGTVASAI"
BASE COUNT          281 a          114 c          157 g          365 t
ORIGIN      
        1 taagcctaaa ttcggcaatt aataatgcaa gttttgacta taaagatgac ataaatgaat
       61 ttatgtcatc ggtactttct ctagctacac tttcgttaat tgtgttctta ttaataggga
      121 acatatattt tttgttttca aatgcttttt ttgatatgaa ccaacttgta tttaatttga
      181 tgttattaca aagttttgct agttttgtaa ttagttatat ccagtcttat tttacgatta
      241 actttaacca ttttaaatat ttgattttat cttttatatc tacttttttg aatattgcaa
      301 tctctgtagc tttgatgcag acattatatt ttgatgataa atatgttggc agagtagtcg
      361 gcggaacact gtcatttgtg ttgataggtt tagtaattta tttaagaata atgataaagg
      421 gtgctcattt ttttgataaa aaacagtgga gatacgcttt aaaaatctct ttgccgctaa
      481 tcccacattc cttagcaaat atagttttag cacaattcga tagaattatg attaattctt
      541 atttaggtcc tgctgcgggg ggaatatatt cctacattag taacataggt attattctaa
      601 gcgtaatatg gatgtctact aataacgcct gggttccatg gttctatggt gagatgagta
      661 aaaataatga aaaaaaagtg aagaaagtta gtaactatta tttagtagtc tttactttga
      721 ttacgatgat tgtaatggta gtttcgattg atttagcaaa atttatggct ccgaaagact
      781 attattctgg gttatcttta gtggttccga taaccttagg ctatttcttt cagtttttat
      841 atagtttacc agttaatgct gaattttatg agaaaaaaac atcgtttatt gcgattggta
      901 cagtagcaag tgctata
//
LOCUS       DACDSY010000334          914 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.347, whole genome shotgun sequence.
ACCESSION   DACDSY010000334 DACDSY010000000
VERSION     DACDSY010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.347"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(231..779)
                     /gene="aac(6')"
                     /locus_tag="IWK44_003057"
     CDS             complement(231..779)
                     /gene="aac(6')"
                     /locus_tag="IWK44_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9551492.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          255 a          199 c          135 g          325 t
ORIGIN      
        1 gccagtttct cggtattaag ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa
       61 tttttcttac tactcgaaac taagcgactt tttcacaacc tctggtacac ggtgttccaa
      121 aagtagcttc ttcggaaata agttggaatt tcacaaaaat tcaagaagta attttcgtaa
      181 attccttctt atttcttgaa gctgattact tctgtcacaa cctcttttta ttattgagaa
      241 tttggtcgag gaataatcgt ttttgccatc caaatatccg gtttgtccca gccatttgca
      301 tttggtaaga cacctacgat tttataacct aatttttcat agaattcata cggatgttca
      361 cgaaggttct ggatggaagc cactttatca aatgtatgct catacaggtc cgtttgactt
      421 aacgttgttc catggtctaa atcatccgta cctaaataaa tcgtgattcc tcctctggaa
      481 gctacttctt tttctaagta attgactaat cgagtaccta tttggttctt tcgtcgggag
      541 ctttctacaa ctaatggatg caattcccaa cctgtgatac cgtattgagg gattgcacca
      601 ataaatccta ctaactcatc ttggtctacc gctgctaccg cgattcgttc tggattcatc
      661 atttcttcta cttcttctgc cgagctgtct ccatattctt ccggccaagt cagtctcagt
      721 aaatcagaaa gctgatcttt caatactgga ttattacggt caaattcact gattatcata
      781 cgtcttcccc cttgttcttg cttattctaa cagtcctttt gaaaaaacga aatgttctcg
      841 ctcaaaaatc tttagctacc aatcagtatc tctattcttt cattatttat cttacttata
      901 aaataaaaaa atta
//
LOCUS       DACDSY010000335          911 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.348, whole genome shotgun sequence.
ACCESSION   DACDSY010000335 DACDSY010000000
VERSION     DACDSY010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.348"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            209..841
                     /locus_tag="IWK44_003058"
     CDS             209..841
                     /locus_tag="IWK44_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9551493.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT          329 a          127 c          138 g          317 t
ORIGIN      
        1 attctaaatt aaatcagtaa aaatttttat tctaagattt ttattagaat tacgcctaat
       61 ttgtgtctct cagggcaatt ctaagaacgt ttcaagagat agcgacacta tcgttatcga
      121 aaaaggcgaa aacctatctc attttctgct aatgtaagtt ggtttagctt ttgttaatat
      181 gactttatga aaatggaggt aattaaatat gatttttcct aaagttaatg aagcaagctt
      241 gaataaagga tccttttttc atatctatga aaacgaagat tttgaactgt ataatatcct
      301 ttcacaaaaa gaggtcatcg aaaaccaaat aatctgccat ccaattattt acaaatctat
      361 tttcaagtat gcattttttg cttccaatga ttttcaaaga atggagatta ccgactgtat
      421 attcgaaaac tgtgattttt caggctgtat attgacaaaa agcatgttgc atcgcgtgaa
      481 atttgtcaat tgtaaattaa caggtactaa attgatggaa agttatattg ggaacacgtt
      541 attcgagaat tgtaaaatgg actatgtaaa tttatctggt tcaaacataa aagaaagtac
      601 ctttgagcac tctgtcttat caagcgcaga ttttgtagac tgttccctca ctaaaataat
      661 gttttctact aatgatttag aaaatgctag cttttttgat actgatttaa aaaatatcga
      721 tttaagtagc aactcctttg aaatgattga agtacgctcg gaacatctaa aaaattgtac
      781 gcttaatcag ttgcaagcac ttggttttgc tagaaaattt ttacaaataa aagtagtata
      841 atcaaaatga atatgtaaga taaattttta gcaatacaaa tatccataaa aaaacattgc
      901 ctattttaac t
//
LOCUS       DACDSY010000336          903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.349, whole genome shotgun sequence.
ACCESSION   DACDSY010000336 DACDSY010000000
VERSION     DACDSY010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.349"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..229)
                     /locus_tag="IWK44_003059"
     CDS             complement(<1..229)
                     /locus_tag="IWK44_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9551494.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            305..377
                     /locus_tag="IWK44_003060"
     tRNA            305..377
                     /locus_tag="IWK44_003060"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:338..340,aa:Lys,seq:ctt)
     gene            539..760
                     /locus_tag="IWK44_003061"
     CDS             539..760
                     /locus_tag="IWK44_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551495.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
BASE COUNT          305 a          145 c          124 g          329 t
ORIGIN      
        1 attttattat agcatgattt ttaccttcat cttgtatttt caatatatac tcaatgacag
       61 cttttttagg tattttgtac cttgctccaa tttttatatt ttctattgat ctatctttca
      121 gcaaattata agctttattt tttccaatat ttaacatttt agttaaatca gtaattttca
      181 atatcgatgg ataactttca aatatttttt cataatcttg aaaatccata aaattcactc
      241 tcaattctga taataagtat tgcacttttt tattttaggt gttagaataa caaagtatta
      301 atttgggctg ttagctcagt tggtagagca gatgactctt aatcatcggg tcgcgggttc
      361 gagcccctca cagcccattg ggtgccaaac ccttaaatac agtgtaaacg ttggtgctta
      421 gcatttagaa aacgtatata ctgtattttt ttgttttcaa aagagactgt agtacccctt
      481 tttttcacta aaagcccact gtagtcattg atatttttga taagaaagga acataattat
      541 gcgtaatgaa attgctaata taacaataat ccgagaaact ttacaaaacc atactgcaaa
      601 tgagatctct aaacatacag gattaaattt gagtactata aaaaaactaa aaagtggaga
      661 acgttcaata gaaaaactaa atttacatga tgctatttgc ctcactgaat ttggtcttaa
      721 aaataataga aaaaatgttg aaatcaacat atggaaataa ttcttaatca gctgcttttg
      781 tcaaaagcac ataggaattt cacctctctc caagtttacg gcgagcctta cggaagtatc
      841 attatcccaa cttcaaatca tcgcttttta ttgactataa aaattatttt agaatgttta
      901 tcg
//
LOCUS       DACDSY010000337          903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.350, whole genome shotgun sequence.
ACCESSION   DACDSY010000337 DACDSY010000000
VERSION     DACDSY010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.350"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>903
                     /locus_tag="IWK44_003062"
     CDS             <1..>903
                     /locus_tag="IWK44_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HAP9551496.1"
                     /translation="DKLYSFINDINSWCSREVQLVNRFIQIGDIYLTKIQKNVLVILF
                     KLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMPLMTSSSEIADKI
                     NRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTRMAIDVANQILLD
                     LPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKKILEDSFPRDTDL
                     VIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIEDMILGMNISKFSN
                     QLNRHFNDNELKVLNNNILKNFSLT"
BASE COUNT          335 a          110 c          157 g          301 t
ORIGIN      
        1 gataaattat attccttcat caatgatatt aatagttggt gtagtcgaga agttcaatta
       61 gtcaatcgtt ttattcaaat aggggatatt tatttaacaa aaattcaaaa aaatgttttg
      121 gttattttat ttaagttaat gtgtagtaaa gaatttcagc aaaaacgttt agcactgatt
      181 ttggctcatg gagatctaac ggcaacctgt atagcaagta ctgctaatag gatcgttgag
      241 agatatatat acgacgccat tgatatgcca ctaatgactt caagtagtga gattgcagac
      301 aaaattaata gatacttaca agagtgtacc ggatatgaag aaattatttt acttgttgat
      361 atgggttcac tggagaaaat ttatctaaat ttggaactag acgctcaaaa aaaattcgga
      421 ctactaaaca atgtttcaac aagaatggct attgatgttg caaatcagat attattagat
      481 ttaccgctta aacagattct tgaacaagta aaagaaaatc aaaatgtgga ttatatttat
      541 gaatcatcaa atattaagaa ggatatgatt gtctgttctt gtgcaagcgg attaggaact
      601 tcgtttaaat taaaaaagat acttgaagat agctttccaa gggatactga tcttgtcata
      661 gaaacgtgtg actattactc tttagtgaat gaaaactatt tatcggagct tcgtaaagag
      721 aacaatattc tttttattgt tggaacacta aatccacatc tagaagatgt agaattcttt
      781 tcaattgaag atatgatttt aggaatgaat ataagtaaat tctccaatca attgaataga
      841 cactttaatg ataatgaact gaaagttttg aacaataata ttctgaagaa ttttagtcta
      901 acg
//
LOCUS       DACDSY010000338          870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.351, whole genome shotgun sequence.
ACCESSION   DACDSY010000338 DACDSY010000000
VERSION     DACDSY010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.351"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..689
                     /locus_tag="IWK44_003063"
     CDS             <1..689
                     /locus_tag="IWK44_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP9551497.1"
                     /translation="TIICNSQNDPAKEREYIEMLEANQVDGIISSSHNLGIADYERVR
                     APIVAFDRNLAPNVSIVSSDNFEGGKLAAKTLQKNGCQNTIMITGNDNTDSPTELRAL
                     GFSFQNPNGKIFKIPNNLSTIRREMEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLK
                     LNIPEDLKIIGYDGTSFIEKFFPQLTTIRQPIDEIASLIVDILLKKIKGEKTNKDYIL
                     PISILSGGSI"
BASE COUNT          313 a          137 c          133 g          287 t
ORIGIN      
        1 aaacgattat ttgcaatagt caaaatgatc ctgctaaaga gagagaatac attgaaatgc
       61 ttgaagccaa ccaagttgat ggcataatct catcaagcca taatcttgga attgctgatt
      121 atgagcgtgt gagagcacct attgttgctt ttgatagaaa tttagctcct aatgtttcca
      181 ttgtttcttc agataacttt gaagggggaa aacttgcagc taagacttta caaaaaaatg
      241 gttgtcaaaa taccattatg attacaggaa atgataacac ggattctccc acagaattac
      301 gtgcactagg tttttctttt caaaatccaa atggaaaaat tttcaaaatt cctaataatc
      361 tgtctactat ccgtcgtgaa atggaaataa agtcaactat tataaatcac aaaccagatg
      421 gtatttttgt ttctgatgat ttaacagcca ttctcactat gaaaattgct catcaactta
      481 aacttaatat cccagaagat ttaaaaatta ttggatacga tggtacctct tttattgaga
      541 aatttttccc acaattgaca acaataagac aaccaattga tgaaattgca agtcttattg
      601 ttgatattct tctgaaaaaa attaaaggcg aaaagacaaa taaggattat attttaccaa
      661 tctcaatact ttctggagga agtatttaac gttaaattaa ttttttaatg tctataaaat
      721 tcgttagaaa atacaatttg tagatatcca attgatttgt aaacaaaaag gagttgtcaa
      781 cttgtagtga cccccaaaag ttagactttt ttggagtgga gaaatccact ccttttttca
      841 tgctctaaaa tgatattttt acacaactgt
//
LOCUS       DACDSY010000339          857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.352, whole genome shotgun sequence.
ACCESSION   DACDSY010000339 DACDSY010000000
VERSION     DACDSY010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.352"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            281..>857
                     /locus_tag="IWK44_003064"
     CDS             281..>857
                     /locus_tag="IWK44_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9551498.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHD"
BASE COUNT          312 a          111 c          152 g          282 t
ORIGIN      
        1 atgttttgat gcttatcaac ttattcaacc cgctatccac ttcaagtata atttttccat
       61 acgccaataa agtagtttta ggagcatgtt ctcatttgag aacgctcctt ttttatatat
      121 tttgaaagct acttactata cgcgtagttt caaatagttt tgtcattcta atcttcagca
      181 acagaaaaaa atctaaacta taaaatttag ggttgactaa aatataattt ttgtatacca
      241 tatgatcgtg ataatcatta tcatttagaa ggagaaagtt atgttagaag tgaaaaagct
      301 taatgtttgt tataacgatt ttttagcatt gaacgagatt tctttaaaaa tacaagaagg
      361 tgctttaaca ggtattattg gtccaaatgg tgctggaaaa tccacattgc ttaaagcaat
      421 gttaaatatt atccctcatc agggagaggt ctccattaat aatgaagata tcaataaaaa
      481 actggtgaag attgcatatg tagagcaaaa agctgatatt gattttactt ttccaataaa
      541 agtaaaagaa tgtgtatcta tgggaacata tgccggaatg aaagtttttc aaagaattaa
      601 aaatgcagaa tggcaaagag tttcaaaggc tttggaaaag gtcgatatgg gaaaatattc
      661 taatcatcaa ataggagaat tatctggagg acaatttcaa cgtgtacttt tagcaagatg
      721 tcttgctcaa aatgcggatt tcatattttt agatgaacca tttgtaggga tagacttagt
      781 tagtgaacga ataattatgg atacgttaaa aaaattgaaa catcagggaa aaacagtgct
      841 aattgtgcat catgatt
//
LOCUS       DACDSY010000340          813 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.353, whole genome shotgun sequence.
ACCESSION   DACDSY010000340 DACDSY010000000
VERSION     DACDSY010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.353"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            533..>813
                     /locus_tag="IWK44_003065"
     CDS             533..>813
                     /locus_tag="IWK44_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9551499.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
BASE COUNT          270 a          130 c          171 g          242 t
ORIGIN      
        1 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcgtaa attagagcgt
       61 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
      121 tgccgaggat aaggagttga cggctaaacc actaatccct gaagctgaca tttgctaaaa
      181 aatggcaagt ccattttttg gccaacctag cgttagcgaa gtttgggggc gcccttttca
      241 aagggtgcgg ttttattttt tggatatttg catagctaaa gagaagtgct attattgagt
      301 tatcaaataa ataaactgaa aaaagagcaa aaaaataaag tcgagcaacg ctggacacgt
      361 tctcgacctt tacggaaact aatcgcacta gctccgtaac ttcttaatca ttatacgaat
      421 atttttagat ttcttcaaga ctagagattt tttggtaatg aatttttaag agttacgtga
      481 acttttgaaa attctttcca gaagtctctt tttctttgag gaggaacaaa atttgagtaa
      541 aaaaggcacg atcataaatg agtgggaaaa attaacagcc ccctctaaca ggcggttaaa
      601 gtatcaaagt ttgacccttc ccttaaaaat gcagtggtca attgaccgaa ttaccatagt
      661 cggaaaactg aaagaaaata tttattatca cactccaaat gatgtgttga ttttaaattt
      721 tgaacagctt atgcgcctaa atgaaggtaa tggttattta agatctgtta gtaataatgg
      781 ttggcagttg ttagaccaac atgaagagaa tat
//
LOCUS       DACDSY010000341          811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.354, whole genome shotgun sequence.
ACCESSION   DACDSY010000341 DACDSY010000000
VERSION     DACDSY010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.354"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IWK44_003066"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IWK44_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP9551500.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DACDSY010000342          810 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.355, whole genome shotgun sequence.
ACCESSION   DACDSY010000342 DACDSY010000000
VERSION     DACDSY010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.355"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            33..446
                     /locus_tag="IWK44_003067"
     CDS             33..446
                     /locus_tag="IWK44_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP9551501.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            583..>810
                     /locus_tag="IWK44_003068"
     CDS             583..>810
                     /locus_tag="IWK44_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551502.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFF"
BASE COUNT          277 a          128 c          138 g          267 t
ORIGIN      
        1 ataaaaaata cttattgaaa ggatgatcaa aaatggcaaa tgtacctagc attcgcgaca
       61 tgttccctga tttcaatgac gtgtttagtc cagcattcaa tgatttttta ggtgtttcaa
      121 gttatccaaa agtagattta gtcgaaaatg aaaaagaata caaactaact gctgatatgc
      181 ctggctgtga caaagaagac acgacagttg aatattcaga taatactttg acgatctccg
      241 ccaatcatga gtcccataca gaagataaag aagatggcaa ctatgtacgg aaagaaagac
      301 attcggtctc ttacaaacgt tcattctatc ttcctaatgt tgatgaagaa aaaatcactg
      361 gaacctttaa aaacggtgtg ctgaaattgg tattacctaa aacagcttat cagccaaaag
      421 aaacaaaaaa aattgagtta aattagaact tatcgcctct cgatgaatga ggggcgattt
      481 ttatatattt ggataagaac gagtatgttt aaattttcaa aatgagcaaa aaaatatttt
      541 gtttttacga taaagatgtt atgatctctc ttggtaaaaa atatgggagt tgatcatttg
      601 attgtcttaa gtaaactttt gttttggata ccatttatca taatgaccag tgctttcttt
      661 tacttattca aaaggcctaa caaatactta ctcttatctg cttgtccgat gatctatttt
      721 atccataaga ttagaggatt ttattattac gactctggta tctcattgtt ttgctacgaa
      781 atcggcttat tagcttcggc catctttttc
//
LOCUS       DACDSY010000343          785 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.356, whole genome shotgun sequence.
ACCESSION   DACDSY010000343 DACDSY010000000
VERSION     DACDSY010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.356"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..>785
                     /locus_tag="IWK44_003069"
     CDS             2..>785
                     /locus_tag="IWK44_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP9551503.1"
                     /translation="MNKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGFNSGN
                     SRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQKGITM
                     SEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIPLKRQ
                     TVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVVTDGL
                     SGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT          266 a          116 c          146 g          257 t
ORIGIN      
        1 aatcaataaa ggtgatttgg atgatttgtt tcgtcgtcac ctagaattgg ctattaacgc
       61 tttattacaa gctgaattaa cggtatttct tgactatgaa aagtatgata gagctggatt
      121 taattcaggt aattcccgta atgggaatta ttcgcgctct tttaaaacag agtacggtga
      181 attgaattta gtaataccta gagataggaa tggagacttt tctcaacaaa ctttacctgc
      241 ttataaaaga accaatgact ctttagaaac cactattatt cagctagttc aaaaaggaat
      301 tactatgtcc gaaatctctg atctaatcga aaaaatgtat ggtcattatt acaaaccaca
      361 aactatttca aatatgagta aaattgtgtc tgaagatgtt tcggcattta aagaaagaac
      421 tttagaagct aaatactcag tcatttttat ggatgctact catattccct taaaaagaca
      481 aaccgtatca aaagaagctg tctatatagt aattggtatc aggttggatg gaactaaaga
      541 ggttctagga tttactatag ctccaactga atctgcttat gtttggaaag agattcttca
      601 agatctaaaa gaccgtggtt tagaagaggt tttgttagtc gtaacagatg gtttaagtgg
      661 tattgacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat gttgtgtcca
      721 tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa tctgtagcga
      781 tttca
//
LOCUS       DACDSY010000344          777 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.358, whole genome shotgun sequence.
ACCESSION   DACDSY010000344 DACDSY010000000
VERSION     DACDSY010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.358"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            205..630
                     /locus_tag="IWK44_003070"
     CDS             205..630
                     /locus_tag="IWK44_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551504.1"
                     /translation="MIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMS
                     MPASSSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRE
                     RMYEKGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT          298 a           85 c          166 g          228 t
ORIGIN      
        1 atcgatgata tttttatgct ttatgatatc gttttttata gaaaattaga tataccgatc
       61 aaacagatga agaatttata tggaaaaaca ttgacggagt tatatgagac acttgatgaa
      121 acagaaagaa gaatccataa agaactggtg gtgatgaaac aaaaacaaaa agagataagg
      181 gagcaaaaaa aacagttgaa attgatgatc gatacaaacg aggaagaatt tcctgtagaa
      241 gagatccctt ttgactgtat gatctcaacg gagtttgaag atattgtgga aatcaaaaaa
      301 tttctaccta actattcaag ctttgggatg atgagtatgc cagcttccag ttcacagacg
      361 atgtatggat tttttatcga ccctagtgaa gtacatttat ttactcaaga cgtgatttgg
      421 gagaaaaaag atacggctgt ttatcgtcga ttcttattga aatccgagat gaaccatgcg
      481 gaacgtaata atattttaga aatcagagaa agaatgtatg aaaaaggatg gaaaacagga
      541 gaagtgatcg gccagtatct attgacgaat acggatgaaa acaatatcag aactgaatat
      601 tatcatgcat ggatagaaat gaaaaaatag gaaatatgtg tttaagataa ctcgaaaatg
      661 atggttgttt tagttataaa gaaaaatttt ctggttcgat tacgggttta atgtggggga
      721 ttattagtcg tggatagata gaattttagt tttttagagg aaatatgtct ttaaaaa
//
LOCUS       DACDSY010000345          776 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.359, whole genome shotgun sequence.
ACCESSION   DACDSY010000345 DACDSY010000000
VERSION     DACDSY010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.359"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..618
                     /locus_tag="IWK44_003071"
     CDS             <1..618
                     /locus_tag="IWK44_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9551505.1"
                     /translation="FLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTATLDD
                     LTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNRLLLT
                     FQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTKDHAN
                     KPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
BASE COUNT          260 a          139 c          117 g          260 t
ORIGIN      
        1 tttttgcatg gtctaggcca agacgaacaa tcatggaaag atgtaatctc ttatctaccc
       61 aatagttatt cttgcaaaag tctttcattg ttcaatcatt taaacagcac agacactgct
      121 actttagacg acttaaccaa atacatcgaa gtacaattga atcagataaa ggaacctttt
      181 attttatgtg gattatcttt aggcgctgtt attgctttaa actatcttgc aactccttct
      241 ccatatttaa aaaaggttgt tttagtcgct ccacaatttg agtctcctaa tcgcttattg
      301 cttactttcc agaatattta tttttactgc gtaccttcta agatatttga aaaattaggt
      361 ctatctaaaa aacaaaccct taccctactt cgttcacttg aaacattaga ctgcaaaaaa
      421 cagcttcgta catcacgact tcctaaattg attttatgcg gtaccaaaga tcatgcgaat
      481 aaaccagcag caaaacgttt atataaactt tcaacgaatg gccggctact ctttattaaa
      541 aacggaaaac atgaattgaa tagagaaaag ccagaaataa tggcacaagc tatgcgatct
      601 tttatcgaaa atagttaatt taaaaaaaga cgacaaaatg tgtcgtcttt ttatttacca
      661 tggtaagaat gttgtcttgt tttttcaata cagcttgatt gagactagtt ttatggacaa
      721 tagagagaaa gttattgttt tattccagag aatttttctg gtcgtagatc tactaa
//
LOCUS       DACDSY010000346          755 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.360, whole genome shotgun sequence.
ACCESSION   DACDSY010000346 DACDSY010000000
VERSION     DACDSY010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.360"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(177..743)
                     /locus_tag="IWK44_003072"
     CDS             complement(177..743)
                     /locus_tag="IWK44_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9551506.1"
                     /translation="MLGLLFAPSIISIFSLTITGFIAFTLVFGKKDTKKMFSKPVAPV
                     KNIAKYFFINVVISAAVSVFLQQILKWGLTGNPINEAFSPLLFIILPIMILGEELFSV
                     YFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNGNILHTVAHILLIQGVARLLFNQAA
                     IKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          277 a          124 c          136 g          218 t
ORIGIN      
        1 ttggtagagc aacgcactcg taacgcgtag gtcgtaggtt cgatccctat agctggcatt
       61 aaaagagaag acagatatat caaggatttt taatccattg atacatctgt ctttttgttt
      121 aaaggaaata aagtatgctt tcttagcaat agagcatgta ttatttctat aaaaatttag
      181 gagaataaaa cgactagcaa tatagctgtg aaatcaaata tcacatgaac tgcccaactt
      241 gtaatgatcg agttgctttt aatggcagct tggttgaata acaatcttgc caccccttgt
      301 atcaaaagga tgtgtgctac agtgtgtaaa atatttccat tatcataagt actgtaatgg
      361 ataaatccaa agatgattgc tgacaagaaa ctagcgacag gaatgctgaa cttactagaa
      421 aaaatagcga gaaaatacac tgagaacaat tcttctccaa gtatcatgat aggcaaaata
      481 ataaataata acgggctgaa agcctcattt attggattgc ctgttaaacc ccatttcaaa
      541 atttgttgca aaaataccga gactgcggca ctgattacta cattgataaa aaaatatttg
      601 gctatatttt tgacaggagc gacaggtttc gagaacattt ttttcgtatc ttttttacca
      661 aaaacaagtg taaatgctat aaatccagtg atagttaaag aaaaaatgct aataatggat
      721 ggcgcaaata gcaaccctaa caaaccgagt acaac
//
LOCUS       DACDSY010000347          736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.361, whole genome shotgun sequence.
ACCESSION   DACDSY010000347 DACDSY010000000
VERSION     DACDSY010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.361"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..385
                     /locus_tag="IWK44_003073"
     CDS             <1..385
                     /locus_tag="IWK44_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAP9551507.1"
                     /translation="QVEKLSHKSYKDVYDEYSKETDIYRELKRDVEALKEIPKNLTDS
                     VDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKIQTIINEMSDEIDSYRAELNKSDWK
                     VNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            388..>736
                     /locus_tag="IWK44_003074"
     CDS             388..>736
                     /locus_tag="IWK44_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAP9551508.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDI"
BASE COUNT          295 a           91 c          140 g          210 t
ORIGIN      
        1 acaagtagaa aagctatctc ataagagtta taaagatgtt tatgatgagt attcaaaaga
       61 gacagatatt tatagggaac tgaaacgtga tgtggaggca cttaaggaaa ttcccaaaaa
      121 tctaactgat agtgttgaca aaattgactt tggcaatggt ataaaagtta cagacgaaat
      181 tgatgaacac cgcggagaat tgattaagaa tttagtgacc aatcgggcta agattcaaac
      241 aataattaat gaaatgtcag atgaaattga tagttataga gcagagctga ataaatcaga
      301 ttggaaagtt aactataatg aggtttcaaa gaggtatcaa agaggtatca aaaacttagt
      361 gaagagttat ctcaagatga attaaaaatg ttacaaaacc ttgattcttt atatcaatct
      421 ttatcaaaaa ctgaagagcg attggaagaa attattcaac aagaaataaa gcttaaagat
      481 taccaagttg aggttgactc agcaatgtca agaatgaata aatcatatca aaatctctat
      541 tctgagagaa gtagttcctc gcgcgaaatt ttttctgata ttacaggaat agaggctact
      601 attcaaccaa atagggattt caataattat gttaaagagc tcagagcttg tattaataag
      661 gaggacactt ttgacagtga gtttggatct cttattgata agctgacaaa aaattccatt
      721 actaatcaag atatat
//
LOCUS       DACDSY010000348          736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.362, whole genome shotgun sequence.
ACCESSION   DACDSY010000348 DACDSY010000000
VERSION     DACDSY010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.362"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..668)
                     /locus_tag="IWK44_003075"
     CDS             complement(<1..668)
                     /locus_tag="IWK44_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9551509.1"
                     /translation="MKFCIKRNVASHYLNQLFSDGKLLKNDSVRPVMFKYNQQKPKDC
                     FSKFIGADISLKSTIDKCKATVMYPPNGLPLIIKGNSGVGKSFLASLIYQYALDRKVI
                     HNDAKFVVVNCADYANNPELLSAVLFGYKKGAFTGAEKDTAGMLSSANGGYLFLDEVH
                     NLSAENQEKLFLLMDSQKYRKLGESVQWEYANVRLILATTEDKNTSLLATFRRRIPAE
                     ITLP"
BASE COUNT          231 a          135 c          112 g          258 t
ORIGIN      
        1 tctggcagag tgatttcagc tgggatgcgt cttctaaaag ttgctaacaa agaggtattt
       61 ttatcttctg ttgtggctaa gataaggcga acatttgcat actcccattg cacactttca
      121 ccaagtttac gatacttttg agaatccata agaagaaaca atttctcttg gttttctgca
      181 cttagattgt gaacctcatc taaaaaaagg tatcctccat tagcagaaga tagcatccct
      241 gcagtatctt tttcagcccc agtaaaggct ccttttttat atccaaataa aactgctgac
      301 aatagctctg gattatttgc ataatctgca caattcacaa caacaaattt tgcgtcatta
      361 tgaattactt ttctatcaag agcatactga tagatcaaac ttgctaaaaa actttttcca
      421 acgccactgt ttccttttat tattaatggg aggccattag ggggatacat tacagtagct
      481 ttgcatttat ctatagtgga tttcaaagat atgtctgctc caataaactt agaaaagcag
      541 tcttttggct tttgttgatt atacttgaac attactggtc taacagaatc attctttagt
      601 aattttccat ctgaaaataa ctgattcaaa tagtgggaag cgacatttct tttaatacaa
      661 aatttcattg caatatcttg agcagaaaaa tcgtaatact ttaaattatt tttgatatac
      721 tctaatactt catctt
//
LOCUS       DACDSY010000349          733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.363, whole genome shotgun sequence.
ACCESSION   DACDSY010000349 DACDSY010000000
VERSION     DACDSY010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.363"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..>733
                     /locus_tag="IWK44_003076"
     rRNA            2..>733
                     /locus_tag="IWK44_003076"
                     /product="23S ribosomal RNA"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DACDSY010000350          716 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.364, whole genome shotgun sequence.
ACCESSION   DACDSY010000350 DACDSY010000000
VERSION     DACDSY010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.364"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..550
                     /locus_tag="IWK44_003077"
     CDS             <1..550
                     /locus_tag="IWK44_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9551510.1"
                     /translation="SLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKNE
                     YLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQD
                     RELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKN
                     PNMFVNYQNDPYQFTTQVILLF"
     gene            565..>716
                     /locus_tag="IWK44_003078"
     CDS             565..>716
                     /locus_tag="IWK44_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAP9551511.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLK"
BASE COUNT          289 a           86 c          116 g          225 t
ORIGIN      
        1 gagtttaaaa gaaatcgcta atcaaaacca tgattcgata ttggaaaaag aaataaaaga
       61 attagagatc tattctaatt cttatctaac caataagttt gaatatcgtt atttaaaaaa
      121 tataaaaaat gaatatttga aaagtttgtt gatagcgaag ctataccaag caaataacaa
      181 taagatttgt tgtaaattcg aatgtccaag tactattatt gaagttcctg ttccaatatt
      241 tgactgtatt agggtgcttg gaattattct cgataatgca attgaagcat ctatagagag
      301 tcaagataga gaactgtctc tagctattta ccgagatgat aaacagatag aaatttcgat
      361 atcgaatagt tgtagagaga tactactctc tatagatact ctaatgaaaa aaggaataac
      421 aacaaaaaag ggacatcaag ggttaggttt atctagtata gaggaaatca ataaaaagaa
      481 tcccaatatg tttgtgaact atcaaaacga tccgtatcaa tttactactc aagttatttt
      541 gttgttttga gggaaggagg atttatgagt tatccaataa tattatgtga agatcaaatt
      601 atacaattaa atcagttaga acgaattatt gataatttca ttctttttca cgataaagta
      661 tttaagatag tattgaaaac acaaagtccg ttagaagtta aaaaatacct caaaca
//
LOCUS       DACDSY010000351          715 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.365, whole genome shotgun sequence.
ACCESSION   DACDSY010000351 DACDSY010000000
VERSION     DACDSY010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.365"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            34..663
                     /locus_tag="IWK44_003079"
     CDS             34..663
                     /locus_tag="IWK44_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9551512.1"
                     /translation="MRQQIIYADLEPGKKVSEKKLEETLSIGRTPIREALIQLRHQGL
                     VDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQKKQVLETIIAEQEK
                     AVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERFRWLRVKTEGLKWEK
                     IMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPKYFKNI"
BASE COUNT          271 a          112 c          114 g          218 t
ORIGIN      
        1 aaaactagtc tccaaaatca agcttatcaa tctattcgtc agcaaattat ctacgctgac
       61 ttagaacctg gaaaaaaagt atcagaaaaa aaattggaag aaacgctttc tatcggtcgt
      121 actccaatac gtgaagcatt gattcaactc cgtcatcaag gacttgttga tacaattcct
      181 caaagtggaa attatatctc attaatcgac ttagatttag caaaaaatgc tcgttttgtc
      241 agagaacatt tagaacgaca aattatgcta gagtgttgtg ccaagatgaa taatcaaaag
      301 aaacaagttt tagaaactat aattgcagaa caagaaaaag ctgttagtca aaagaatgct
      361 cgtagttttt ttcataatga taatttattt cataaagctt gttttgaaat tgcagaacgg
      421 gaagaagtat ggcaatggtt ggaagataat aatacgcact tggaacgatt tcgttggtta
      481 cgagtaaaaa cggaaggatt aaaatgggaa aaaattatgg aacaacatca tcaactattt
      541 aaagccttga ccatgcaaga ccctgaagaa gctaacttct taacatcctt gcatcttcat
      601 ttaatgcttg atgaacaaaa tgttgtattg actactcacc caaaatattt taaaaatata
      661 taactgctaa atttcttttt tttaatttat ttacatttct gattactaca gaaaa
//
LOCUS       DACDSY010000352          714 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.366, whole genome shotgun sequence.
ACCESSION   DACDSY010000352 DACDSY010000000
VERSION     DACDSY010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.366"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>714
                     /locus_tag="IWK44_003080"
     CDS             <1..>714
                     /locus_tag="IWK44_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9551513.1"
                     /translation="VATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVY
                     ISVFRRCKDFKDNEVWNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSV
                     AYSIGALINLVTNSVNSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSK
                     EIVLIFGGQKYIDSVNLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMN
                     IVLNYVFIKLYGYQAVAYTT"
BASE COUNT          194 a           99 c          150 g          271 t
ORIGIN      
        1 gtagctaccc ttgcagttac tgttggctca tctgtagttg gctgtttagc ggttctgttt
       61 atatctccta ccgctgaggc gagagcctta atgaatgtcc tagtgactgg tgttgttggc
      121 gtggctgtgt atatatcggt atttagaaga tgtaaggatt ttaaggataa tgaagtgtgg
      181 aatttcgcag tagtattttg tgtatcgttg atgccacact atttatctga atttattctg
      241 aatggttctg ataaaattat gataaattac ttatgtggtt cgtcagatgt tgctatttac
      301 agcgtggcat attctattgg tgctctaatt aaccttgtta caaattctgt caattccact
      361 tttgcacctt atcagtatca aaaaatcaag tcaggagaat atgatttact atcaaaaaga
      421 gctaatgaag ttcttggttt ggtagcggct atgctttttg cattaatgct atttagtaaa
      481 gaaattgtgt tgatatttgg tgggcagaaa tatatcgaca gtgttaattt aattgtacct
      541 atatgtatcg gtatttattt caactatatg tttcagctgt ttgctagagt acaggaatac
      601 tttgaaaaga aactcactgt cgttattcca tcggttttgt gtgcaattat gaatattgta
      661 ctaaattatg tttttataaa gttatatggg tatcaggcgg tagcatacac aact
//
LOCUS       DACDSY010000353          712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.367, whole genome shotgun sequence.
ACCESSION   DACDSY010000353 DACDSY010000000
VERSION     DACDSY010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.367"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..446)
                     /locus_tag="IWK44_003081"
     CDS             complement(<1..446)
                     /locus_tag="IWK44_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9551514.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DACDSY010000354          707 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.368, whole genome shotgun sequence.
ACCESSION   DACDSY010000354 DACDSY010000000
VERSION     DACDSY010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.368"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..461)
                     /locus_tag="IWK44_003082"
     CDS             complement(<1..461)
                     /locus_tag="IWK44_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9551515.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          225 a          128 c          101 g          253 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
      541 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
      601 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
      661 agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgat
//
LOCUS       DACDSY010000355          706 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.369, whole genome shotgun sequence.
ACCESSION   DACDSY010000355 DACDSY010000000
VERSION     DACDSY010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.369"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            11..634
                     /locus_tag="IWK44_003083"
     CDS             11..634
                     /locus_tag="IWK44_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551516.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
BASE COUNT          239 a          111 c          152 g          204 t
ORIGIN      
        1 agaaaagaag gtgtttgtta tttgggaagt agttaaatcg agagtacaag aaatgaatga
       61 acatttctca gtgtcattcg cagaaaatcc aagtaagtat tttcgacgtc ttgtttttct
      121 gctcatctta tcattgctgt taacagttgc aatgttttta ttgccggaaa aaatccgaga
      181 agagcaagta aatagtctcg tttattcgtc cataccaacc agcaaattga aaccttttga
      241 ttacagcaaa gctgatcaga aaatcaaaca aagccgggcg atatcggtga tgttcagcaa
      301 accgaacggg aaaacctatc aggacatgct tgatgttttt aatgatcctg agcttatgga
      361 agaattgaat cgaccgatct tttattatcc gatcgtttat gatgtacatg aactggaaca
      421 aaaatataac atacgaacgg atgaagtgac atttattttc tttgatggag gaaaagaagc
      481 aaatcggata actgcgggta agggaggaat aactgatttt gataaggagt tgatccctga
      541 actgaatcga cttccgttag ccaatatcaa acaattagaa gaagaattgg atagaacggg
      601 agtaacttcc caaaattccg agattgctaa ttgagacatt ggcgcgatga aacggtgggt
      661 ctatatagcg ctatttttaa ttatcagaac aagtgatgtt tgatct
//
LOCUS       DACDSY010000356          702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.370, whole genome shotgun sequence.
ACCESSION   DACDSY010000356 DACDSY010000000
VERSION     DACDSY010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.370"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            161..271
                     /locus_tag="IWK44_003084"
                     /pseudo
     CDS             161..271
                     /locus_tag="IWK44_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(368..>702)
                     /locus_tag="IWK44_003085"
     CDS             complement(368..>702)
                     /locus_tag="IWK44_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9551517.1"
                     /translation="KIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGE
                     PFSICVQENDEVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHG
                     NSCGKITF"
BASE COUNT          237 a          121 c           85 g          259 t
ORIGIN      
        1 catctttcag cattttacta tatttggtat atagtgctaa ggttcatatt cgattgacaa
       61 gagaatataa attgatctat ttctatttcc taagttgagt aaacataact ttgcccttta
      121 taaatatctt ccctctctct cttctctcta tattttatat ttgaataatc atatcaaggt
      181 attccaacga aatgccaatg gatttcgaag cttttataac tttaaattac ggatcatgat
      241 ttggcatggg gaatcattac taataaaata aaaaaatgaa cagaaagaaa acagttcttt
      301 ctgttcacca acttcatttg acaaatatcc tatttagctt attttatagc tttttatata
      361 cactttttta aaaagtgatt tttccacatg aattcccatg agcaattcga ccattatcta
      421 ttattaaatc attgaactga tttttctccg gaaaaacaac tataacagaa gtatctttac
      481 cagattttat tattttgtca aaatccacat caaccaacga ggtttcagga gtaacctcat
      541 cattttcttg aacacaaatc gaaaaaggct ctccttgtaa ttcaactgta tcaattccaa
      601 tatgaatcaa ataatcaatg cctgagttgc tttttatatt aatagcatgt ttagtaggaa
      661 atatatttgt tactcgacca gccaatggag cataaatttt tg
//
LOCUS       DACDSY010000357          700 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.371, whole genome shotgun sequence.
ACCESSION   DACDSY010000357 DACDSY010000000
VERSION     DACDSY010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.371"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(438..662)
                     /locus_tag="IWK44_003086"
     CDS             complement(438..662)
                     /locus_tag="IWK44_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551518.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          255 a          100 c          129 g          216 t
ORIGIN      
        1 gggttgagag aaatctaatt gattttcaaa gaaggagtat gaaaatgaga aaaatattta
       61 ttcgtttatt gtgggtacta tcttagagga ataaataata gtggagaaaa ctatggtttg
      121 gcgcagattc tatcaggttt gatggaacga agacaacctc ccgacatcaa gtcaaaagct
      181 acttgagatt ttgatctgaa aataaaaaaa gaaaacaagg taggaacatc aatgtttcta
      241 ccttgttttc gtaatcaatg ggaaaaaact aagagtggtg tacgtaatca ataaaaagga
      301 tggtgacaac agtacctctt tactctctga aagcatcata actcgttttt cctcattatg
      361 ccaataaatt gttgttatac cacatataag gatttgtaat agctaaaaac agacaggaaa
      421 gttgaaatct gtgaatctta ttttgtatgg aaggatttat agaacgcaaa aatcaagata
      481 aagatgaata aaaatcctgt ttcggctata tattgcgtat ctacgccaat aaaacgagtt
      541 tgttctgtca tagccatata aagcaaaggc aaaaatacaa ctacagcgag ataagatccg
      601 atgatttctc gaaaagtttt ccaggaagta tgtttcctgt ttttatattt atccgatttc
      661 attgaatagt catagcctcc ttattctttc atatgttgaa
//
LOCUS       DACDSY010000358          694 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.372, whole genome shotgun sequence.
ACCESSION   DACDSY010000358 DACDSY010000000
VERSION     DACDSY010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.372"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            87..356
                     /locus_tag="IWK44_003087"
     CDS             87..356
                     /locus_tag="IWK44_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9551519.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            349..606
                     /locus_tag="IWK44_003088"
     CDS             349..606
                     /locus_tag="IWK44_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9551520.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          270 a           85 c          129 g          210 t
ORIGIN      
        1 tataagaagt tagaatatta atcatttgct taattgtaca atataatgta caattgtttt
       61 atagaaataa ataaggggtg aaaggaatgg aagcagtagc ttattcaaat ttccgccaaa
      121 atttacgtag ttatatgaaa caagttaatg aggatgctga aacacttatt gtaacaagta
      181 aagatgtaga agatacagtt gttgtattat caaaaagaga ttatgattct atgcaagaaa
      241 cgttgagaac actttctaat aattacgtca tggaaaaaat tcgtcgagga gatgaacaat
      301 tctccaaagg tgcatttaaa acacatgact taatcgaggt tgaatctgat gattaaggct
      361 tggtctgatg atgcttggga tgattatctt tattggcatg agcaaggaaa caaaagcaat
      421 ataaaaaaga ttaacaagtt aataaaagat atcgatcgtt ccccctttgc tggattagga
      481 aaacctgagc cattaaagca tgatttatct ggaaaatggt ccagaagaat tacagatgaa
      541 catagactga tatatagagt tgaaaatgaa acgatattta tttattctgc aaaagatcac
      601 tattaaccaa tcggaagtaa ggaaagggtc agaaacttaa aagtttttga tccttatttt
      661 atttacccta gtcatttaaa aagctaatat agct
//
LOCUS       DACDSY010000359          667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.373, whole genome shotgun sequence.
ACCESSION   DACDSY010000359 DACDSY010000000
VERSION     DACDSY010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.373"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..122
                     /locus_tag="IWK44_003089"
     CDS             <1..122
                     /locus_tag="IWK44_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9551521.1"
                     /translation="LAALIGISGAYLSDILNGNRDGKKAQQHIETVKKILDIR"
     gene            134..382
                     /locus_tag="IWK44_003090"
     CDS             134..382
                     /locus_tag="IWK44_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551522.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            413..592
                     /locus_tag="IWK44_003091"
     CDS             413..592
                     /locus_tag="IWK44_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551523.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
BASE COUNT          243 a          103 c          141 g          180 t
ORIGIN      
        1 aactagctgc gcttatcggt atttcaggag cttatttatc agatattcta aacggcaatc
       61 gtgatggaaa gaaagcacaa cagcatatcg aaacagtgaa aaaaatattg gacatccgat
      121 aggggggtag gcgatgacaa aactaaaaaa acaagatttt gtaaaaaaat acaattattc
      181 tccatctact tatcaacgtc gaatgtcgga actaaaaaat acagcaattt tctcagcggc
      241 gtatgaacgg gtcacaggac aagaagtttg gatcaataca gaattatacg ataaattttt
      301 gtctttcaaa tcctataaca ggttacgcac aagaaaggta acacctaaag aatttatcga
      361 gaagcattta gttgatttat aaaaaacaga acattttgag aggtgaaggt taatgggtaa
      421 attcaacaga gcattggtat tcagcgcacc gctaatcatc tacgctttag gactttgggg
      481 aagcaggcaa gcgttgatag gaacgattgt ttacatggtt tggattttta tggggcttga
      541 tgaagctgaa gctgagtaca gagcgaaaaa gccaaccgag ggggctgact aaaaatgaat
      601 agaaaagaga aactagaatg tatattacta ttactcagtt taattctttc actaatttct
      661 ctattgg
//
LOCUS       DACDSY010000360          663 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.374, whole genome shotgun sequence.
ACCESSION   DACDSY010000360 DACDSY010000000
VERSION     DACDSY010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.374"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            123..458
                     /locus_tag="IWK44_003092"
                     /pseudo
     CDS             123..458
                     /locus_tag="IWK44_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            554..>663
                     /locus_tag="IWK44_003093"
     CDS             554..>663
                     /locus_tag="IWK44_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9551524.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT          234 a          127 c          132 g          170 t
ORIGIN      
        1 ttttatataa taaaatgcaa tacttttcga gagtgtaaaa tattttgtgt aaatagaaaa
       61 aaggaagtcc cttctgtaga atagagttac cacaacacat tcacagaaaa gaggacttcc
      121 atatgaacga ttttactaca gaaattctaa agactctagc gaacaaaggc gatttgaatg
      181 aattattccg tgtccatttg gaaaaagctg tcaatacgct tctcaaaacg gagttaacgg
      241 ctttcctcga ttacgaaaag tacgatcgca ttggttttaa cacgggtaat tctcgtaacg
      301 gctcctatga ccgtacggtc aagaccgagt acggggaact tcatctccag attccgcgcg
      361 accgcaacgg cgagttcaag caacagactg ttcctgctta tagacggacg aatgatacct
      421 tagaggagac cgtcattcac ctcttccgaa aaggtatgga taggctacaa ttaactagtg
      481 gataggctac aattaactag acagaaaaat taaggtgtgt agactagaag aaaacatacc
      541 aggaggaatt tttatgtcta agagaacacg aagaactttt tcacaagaat tcaagcaaca
      601 aatcgtcaat ctttacttag ctggaaagcc acgtgtagaa atcattcgag aatatgaact
      661 aac
//
LOCUS       DACDSY010000361          657 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.375, whole genome shotgun sequence.
ACCESSION   DACDSY010000361 DACDSY010000000
VERSION     DACDSY010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.375"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..291)
                     /locus_tag="IWK44_003094"
                     /pseudo
     CDS             complement(1..291)
                     /locus_tag="IWK44_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          198 a          119 c          134 g          206 t
ORIGIN      
        1 ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct cgaaacagtt
       61 ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct ccataagtga
      121 attatctaag cagttccctt tccgagacat gctttgaaag attttgtact ccttcaattt
      181 gtttctataa gctttcattt gatacgacca accttgatct gagtgaaacg tacgacgata
      241 gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg cacgtgtttc
      301 tgaatggtgg tagcgaccga tttgaacggc tagggcggaa atacggtgca aatgcacggt
      361 ggtttcatgc aatgcttaca gggcgtattt tgtaagggta tgtaacggag ataatcccga
      421 cggtaatgga tgtgagaggc caagccgacg ctgacggtag ggcaaacaaa catagttgca
      481 ctctagcgat cgttatttat ttttaacggt caaactaggg tacaactatg cttaaaacac
      541 ttcgcctcaa cttctggtag caaaagtgat tttgaaagtc aaaatatttt ttatgagtta
      601 tttataattc gccgtttgta taattaagcg agacaaataa aaaagccatt tatgtta
//
LOCUS       DACDSY010000362          647 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.376, whole genome shotgun sequence.
ACCESSION   DACDSY010000362 DACDSY010000000
VERSION     DACDSY010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.376"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>647)
                     /locus_tag="IWK44_003095"
     CDS             complement(<1..>647)
                     /locus_tag="IWK44_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9551525.1"
                     /translation="MLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFS"
BASE COUNT          207 a          117 c           84 g          239 t
ORIGIN      
        1 atagaaaagc catttgtaaa ttgggaacca gaaggtgtaa aatcttgatt gttaatatgt
       61 tgagttgcaa tataagctgt tcttagagca ttagaaatag aaaaatcaac actacttcta
      121 ttaatggacg taaaaaaagt tgtagcacaa tccccaatag catatatatc ttttatattt
      181 gtttgctggt attcattagt tagatatgct ccactaacat ctctcaagag ttccttttcc
      241 ccaattggac tattagatag aaatcctata gcttgtacga ccatatccat agagtattgg
      301 gtaccacttg tagtgactat tttttcaatc ttttttcttc ctaaatattt tttaggactt
      361 tctgatagac aaagatgtat tccatttttt gatagtattt cttcaacttt atctgaaaaa
      421 ctacggtcgt aataagtcga caacactcta tctgcaacat cgaaaagata tacttctttc
      481 cctctttttc ttatggcttc agcaatttca actccaatat atcctgctcc aataattcct
      541 acttttttaa tagtactatc atccaaagct ttaaaaatct cttttgcatt tggaaaattc
      601 tttatcgaaa aaatattagc taaattattc cccggtatat ctagcat
//
LOCUS       DACDSY010000363          639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.377, whole genome shotgun sequence.
ACCESSION   DACDSY010000363 DACDSY010000000
VERSION     DACDSY010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.377"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>639
                     /locus_tag="IWK44_003096"
     CDS             <1..>639
                     /locus_tag="IWK44_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP9551526.1"
                     /translation="ENIYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVR
                     KDKKLEPQREFIVQIGILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQE
                     RNPNLAVYNAVIHNDEISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNG
                     RVLFNNFRNQEVDSIERLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQT"
BASE COUNT          241 a           91 c          139 g          168 t
ORIGIN      
        1 gaaaatattt atttagagca gacgaatcta gcagataaat atgaggaatt gtttggcgaa
       61 gtggtagaaa aatataatgc gaaacaaaaa agagccgata gaaaaatcga ggactattta
      121 gcgaaagtgc gcaaggataa aaagttggaa cctcaacgag aatttattgt gcaaataggc
      181 attttagatg atttccagac aaatcgagca gatggttctt ctactggcat atcgaaacaa
      241 caagctgaac aaaatcgagt gattgccaat aaaattttgg ttcagtatta caaggagttt
      301 caagagagaa accctaattt ggctgtttat aatgctgtta ttcataatga cgaaataagt
      361 ccgcacctgc acttgaatat tgtgccggtt gctgaaggtt ataaacgggg tgttcaaaag
      421 cagccgagct ttaataaagc actggttcaa caagggatac acgctgataa agataatggt
      481 cgagtgcttt tcaataattt tagaaatcaa gaagttgact ctatagaacg attaatgaat
      541 gattacggtt ggaaaaggga tattgtcggt accaacaaaa ttaaagatat tcacgaatac
      601 aaagaaatca tgagtgaaat ttctgaattg cgccaaacg
//
LOCUS       DACDSY010000364          637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.378, whole genome shotgun sequence.
ACCESSION   DACDSY010000364 DACDSY010000000
VERSION     DACDSY010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.378"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(107..511)
                     /locus_tag="IWK44_003097"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="IWK44_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
BASE COUNT          202 a          121 c           99 g          215 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaa
//
LOCUS       DACDSY010000365          615 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.379, whole genome shotgun sequence.
ACCESSION   DACDSY010000365 DACDSY010000000
VERSION     DACDSY010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.379"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>615
                     /locus_tag="IWK44_003098"
     CDS             <1..>615
                     /locus_tag="IWK44_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP9551527.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQ"
BASE COUNT          210 a          119 c          104 g          182 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc ttctcaacgc ccattaaacg agaattctaa cgggattctg cgtcgtaatg
      481 gactgccgaa atcaatggat tttagaaaag tgaatcagac atttatttcc agtgtcagca
      541 atcaacgtaa tcatattcca agaaaatcat tgaattacag aacaccaatc gaaatatttt
      601 tgagctatgt acaag
//
LOCUS       DACDSY010000366          614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.380, whole genome shotgun sequence.
ACCESSION   DACDSY010000366 DACDSY010000000
VERSION     DACDSY010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.380"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..164
                     /locus_tag="IWK44_003099"
     CDS             <1..164
                     /locus_tag="IWK44_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9551528.1"
                     /translation="ISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYHAN
                     EVLKIAEEK"
     gene            165..>614
                     /locus_tag="IWK44_003100"
     CDS             165..>614
                     /locus_tag="IWK44_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9551529.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPY"
BASE COUNT          234 a           74 c          115 g          191 t
ORIGIN      
        1 aaataagttt tgaagaaaac attgttaaag aaatgcctga atctgctaat gatatgtatg
       61 agaaggagtt gaaatatttc tttgaaaacg tagtaacagg caaagaaaac tggaatgatt
      121 tatatcatgc aaacgaagta ttgaaaatag ctgaggagaa ataaatggaa attttattta
      181 ctatttgtgg tagagctggg tctaaggggt ttaaaaataa aaatttgaaa tatttggcag
      241 gaaagcccct agtccatttc gctttagcaa tgatagatat ctatcaacaa aaacatcctg
      301 aacataattg cacgacttgt ttgaatacgg acagtaatga gttagtagaa ttgacgacta
      361 agttaaatcc gtctgtgaaa attgtagaac gtaaacctga attagctaca gatactgtag
      421 ggaaaattga agtgatacgt gattcattct tacaactaga aagagaagat aatatatttg
      481 atttaattat agatttagat ttgacttcac ctttacgtga agtttctaat cttgaagaat
      541 taatagatga acatctaaaa aatttagata aagacgtcta cttttcagtt gtttctgcaa
      601 gacgtaatcc ctat
//
LOCUS       DACDSY010000367          612 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.381, whole genome shotgun sequence.
ACCESSION   DACDSY010000367 DACDSY010000000
VERSION     DACDSY010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.381"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            290..>612
                     /locus_tag="IWK44_003101"
     CDS             290..>612
                     /locus_tag="IWK44_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAP9551530.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLG"
BASE COUNT          226 a           70 c          100 g          216 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga ag
//
LOCUS       DACDSY010000368          598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.382, whole genome shotgun sequence.
ACCESSION   DACDSY010000368 DACDSY010000000
VERSION     DACDSY010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.382"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(73..>598)
                     /locus_tag="IWK44_003102"
     CDS             complement(73..>598)
                     /locus_tag="IWK44_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9551531.1"
                     /translation="VIRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEY
                     RNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCT
                     KDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLG
                     KAQKIKEKLKESTV"
BASE COUNT          193 a          107 c           72 g          226 t
ORIGIN      
        1 attcagaaaa atttgcacta gtgaaggaat ctattttatt agaaaaatgc aatctcccta
       61 aagaaaaaac cactaaacag tgctttcttt caacttctcc ttaatttttt gtgctttccc
      121 taaagtaaag tttctggcca actctgaatc aaaatatagt gaacgttttt taaaatctat
      181 ttctttaacg ttaaagatat ttactaaaca cgctcgacta attcttgtta acataggata
      241 tttcttttca agctcactaa ttcttccata aaattcgtac tgcccatctt tagtacacaa
      301 tgataatcta tgtggtaatt ttgaagcttc tacaaaataa atgtcattta tatcaaaagt
      361 aaatgtttga gaacctatgg aaaaaacaaa tgcttgattc gtaacctgtt ttgttgcatc
      421 tatcctttct tgcgctaata acaataactc aacaatctcg tttcgatact catctagtgt
      481 ttgatctttt gttacaaatc ctaacgtttc aactcttctt tttattgtta cgggtaacat
      541 ctcatcatga gtagttgtaa aaatgatttt tgcttgaaca tcatattttc tgattact
//
LOCUS       DACDSY010000369          580 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.383, whole genome shotgun sequence.
ACCESSION   DACDSY010000369 DACDSY010000000
VERSION     DACDSY010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.383"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(103..>580)
                     /locus_tag="IWK44_003103"
     CDS             complement(103..>580)
                     /locus_tag="IWK44_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9551532.1"
                     /translation="INRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQ
                     NQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLA
                     NIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          196 a           91 c           70 g          223 t
ORIGIN      
        1 acattaaaag tactaggcat aaaataaagc agacaaatga ttaaatttat tactcgaaac
       61 gcatctcgac ttaataaact cttctctaaa acaggtacta ttctattcat ctatatttcc
      121 aactttcaaa atttctttat caattctatt taatagttta tcagagtaat ccgatttaac
      181 ttttcttaca aataattttt cactttcttt tatttgatct atatcccttt catcaaacca
      241 tttgttcatg gaaggatcaa taatatgaat atttgctaat ttcgaagttt gtaaagtagt
      301 ttcttcaata tttttcaatt caagtggtaa atctggtcct ccaataacat ttttattttt
      361 gaacgaggaa ttaaaaaaaa gtgtatgaaa aaatttttca tctggggcaa acatacctat
      421 tttaaaattc aggtagtctt gttgattttg ttcatacatt tgtattaatt ctgttaggca
      481 ttctttcgac aaagcccacc attgagaacc atgatacaaa tcaaatttat ggtgtctaat
      541 ttttggggga agtttaatca tcctcagaca acgattaatt
//
LOCUS       DACDSY010000370          576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.384, whole genome shotgun sequence.
ACCESSION   DACDSY010000370 DACDSY010000000
VERSION     DACDSY010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.384"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>576
                     /locus_tag="IWK44_003104"
     CDS             <1..>576
                     /locus_tag="IWK44_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HAP9551533.1"
                     /translation="SNVARNIASNIRAIYVDEFQDTRELQYNVIAKLLQNNPQIQSMF
                     VGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCYRSNQRLVDFYSNFQSCPYE
                     IESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIESGIHEKDICVLAPQHYPLFS
                     LGEYLKKELPYCNFDAINISPIKVNNHSLFF"
BASE COUNT          214 a           83 c          100 g          179 t
ORIGIN      
        1 cagtaacgtt gcacgtaata ttgctagtaa tataagggcg atttatgtgg atgaatttca
       61 agatactaga gaattacaat acaatgtaat agctaaatta cttcaaaaca accctcaaat
      121 tcagtcaatg tttgttggag atattgacca agcaatttat ggatcattag atggtatagc
      181 taaatcagtt gaagaactag aaatgctaac tagtcacact tttcaatcaa agacgctaca
      241 tggctgttac aggtctaatc agagattagt agacttctac tctaattttc agagttgtcc
      301 ttacgagatt gaaagcagag ggaataataa aaacgaccgt ggttttataa gttatgattg
      361 caaagttact aaggaggatt tacccaacat aataaaaaat ataataaagg aaaagattga
      421 aagcggcatt catgaaaaag atatttgtgt acttgctcct caacactatc ctctgttttc
      481 actgggtgag tatttaaaga aagaattacc gtattgcaat tttgatgcta taaatatatc
      541 accaattaag gtaaataatc atagtttatt tttcaa
//
LOCUS       DACDSY010000371          551 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.385, whole genome shotgun sequence.
ACCESSION   DACDSY010000371 DACDSY010000000
VERSION     DACDSY010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.385"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>551)
                     /locus_tag="IWK44_003105"
     CDS             complement(<1..>551)
                     /locus_tag="IWK44_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9551534.1"
                     /translation="GCIALMVVTLLLPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLI
                     SNLFFAITGLSFFLVFDLMDYLGKRHLGTASFFALLIAFIFYLLVKSIDKQTRFLNLL
                     FTYPTEHYFGGICILLSWGVIYLVLGSVHFRSSNYLLLSLLSIVISFFSLFFSIMLIS
                     FQVKERQHQESLQLYQINEEYYH"
BASE COUNT          230 a           90 c           91 g          140 t
ORIGIN      
        1 gatggtaata ttcttcattt atttgataca gttgaagcga ttcctgatgt tgtctttctt
       61 ttacttggaa actgatcaac ataatggaaa aaaacaaaga aaaaaaactg atgactatcg
      121 acaaaagaga tagaagcaga taattagatg agcggaagtg aacacttccc aataccaagt
      181 aaatcacgcc ccagcttaat aaaatacaaa tacctccaaa atagtgttca gttggataag
      241 taaaaagcaa gttgagaaat ctcgtctgtt tgtctatact ttttactaat agatagaaaa
      301 taaatgcgat aagtagtgca aaaaaacttg ctgttccaag atgtctcttt cctaaataat
      361 ccatcagatc aaaaaccaga aaaaagctga gtccagtgat agcaaaaaaa agattactga
      421 tcaaacagct gataaacagg tttttcgtag taaaataaaa aagaaacatc aataataagc
      481 caactaggta aggaaagctc actttaggga taggaagtaa aagtgtcacc accatcaaag
      541 ctatacaacc a
//
LOCUS       DACDSY010000372          548 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.386, whole genome shotgun sequence.
ACCESSION   DACDSY010000372 DACDSY010000000
VERSION     DACDSY010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.386"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          195 a           61 c           93 g          199 t
ORIGIN      
        1 gtttgtcaat aggaaaaatt tatttctcta aaaaatataa ataatctgca gtgaaacttg
       61 agtacatata ctaatttcga aaaatgccta tgatttaaat aattatggtg ctgggatgaa
      121 ggtttataag tttgtcttga tttgtttaga agaaatcgtt tttgcacaca gttgaattag
      181 gaaactactg gagtggaaaa taaagtgatt tgacgggtag atgggtgcac ttaaatttga
      241 aaatggctta aaatgcttta ttttgaattg cagaatttct tttagtacgc aatttatggt
      301 cttaaaactg attaaaaagt gatacattga ttttaataca atttatgata caaaaaatga
      361 cctcctttct taaataaaag aggtcatttt gttttgtctc ataatcgatt gtttttgata
      421 cagtagttat attcagaaaa aataaagact cttctgagtt tataatcctt tggattatat
      481 acggatacag atgtagacat aaaacaagct caattttcaa aaatggcatt actcaccttt
      541 ttggtgaa
//
LOCUS       DACDSY010000373          536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.388, whole genome shotgun sequence.
ACCESSION   DACDSY010000373 DACDSY010000000
VERSION     DACDSY010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.388"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(111..194)
                     /locus_tag="IWK44_003106"
     tRNA            complement(111..194)
                     /locus_tag="IWK44_003106"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(158..160),aa:Leu,seq:cag)
BASE COUNT          128 a          113 c           75 g          220 t
ORIGIN      
        1 atttgaatct ggttccgttg tttaaaattt ttcctatatt cttttagata atatgttatt
       61 ttctttctca tcgctccagg tgtaccttct ctttataaaa aaacaaatta tgcggctaag
      121 aagactcgaa cttccacgag gtcaccctca ctagcgcctg aagctagcgc gtctgccaat
      181 tccgccatag ccgcatcaca tcttttagtt taggattaca tgtgtttttt gtcaagaaca
      241 tatttttgtt ctttttcatt tctgtacgcc ctcccttatg ccatctacac aaataattca
      301 tccgagcaac tccatcatct attgagggtt ggtgaaaaaa tctgttttat ccatatgatc
      361 gatttcaatg ttaaacgaac gactctggga ttagtaacta ttttattttt ttcttcattt
      421 cttttttcct ctttttctcg attcagctat cttctttcat ttggatattg attatttctt
      481 ctgttgatta ctatccttct ggaattctag acgctgaagt aattcacgtt ttcttt
//
LOCUS       DACDSY010000374          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.389, whole genome shotgun sequence.
ACCESSION   DACDSY010000374 DACDSY010000000
VERSION     DACDSY010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.389"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(5..90)
                     /locus_tag="IWK44_003107"
     tRNA            complement(5..90)
                     /locus_tag="IWK44_003107"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IWK44_003108"
     tRNA            complement(107..178)
                     /locus_tag="IWK44_003108"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IWK44_003109"
     tRNA            complement(190..262)
                     /locus_tag="IWK44_003109"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IWK44_003110"
     tRNA            complement(280..361)
                     /locus_tag="IWK44_003110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IWK44_003111"
     tRNA            complement(369..441)
                     /locus_tag="IWK44_003111"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IWK44_003112"
     tRNA            complement(445..517)
                     /locus_tag="IWK44_003112"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACDSY010000375          526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.390, whole genome shotgun sequence.
ACCESSION   DACDSY010000375 DACDSY010000000
VERSION     DACDSY010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.390"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IWK44_003113"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IWK44_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP9551535.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DACDSY010000376          515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.391, whole genome shotgun sequence.
ACCESSION   DACDSY010000376 DACDSY010000000
VERSION     DACDSY010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.391"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          153 a           81 c           82 g          199 t
ORIGIN      
        1 agctaacaaa attgtcataa aaaacgagat ggttgatatt agaacttgtg tttttgttta
       61 ggtctagaga taaagagaga caatttccaa atatatgtaa tgaatacaaa aagacgtttg
      121 tcgcggatta ccgttacaaa cgtcttttat tgattttccg agatctcatc agtcgccatt
      181 tggaatggtt cactccttat ctagattcgt ttaatgctcg gactgcttgt tcgattgtaa
      241 taccaaacgc aacgttattt tcgttttgtg catcaatcgc cataaaacgg ttagaagttg
      301 tatcaaaagc tacgcggtat tgcatatttg tttttgtaaa cgtcataaaa ttcatctcct
      361 ttgtaatgta atttagttct tgaaagtttc ctttaaactt tcgaacttat accaagttta
      421 gcatcgtacg ttttttgatg caattaaaaa gcatttatca ttttccttat ttattttttc
      481 ttaaatggtt tagctatttt cttttaatcc gataa
//
LOCUS       DACDSY010000377          514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.392, whole genome shotgun sequence.
ACCESSION   DACDSY010000377 DACDSY010000000
VERSION     DACDSY010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.392"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IWK44_003114"
     CDS             complement(<1..132)
                     /locus_tag="IWK44_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9551536.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DACDSY010000378          510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.393, whole genome shotgun sequence.
ACCESSION   DACDSY010000378 DACDSY010000000
VERSION     DACDSY010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.393"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..510
                     /locus_tag="IWK44_003115"
                     /pseudo
     CDS             1..510
                     /locus_tag="IWK44_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          212 a           88 c           92 g          118 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag
//
LOCUS       DACDSY010000379          502 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.394, whole genome shotgun sequence.
ACCESSION   DACDSY010000379 DACDSY010000000
VERSION     DACDSY010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.394"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(28..426)
                     /gene="spxA"
                     /locus_tag="IWK44_003116"
     CDS             complement(28..426)
                     /gene="spxA"
                     /locus_tag="IWK44_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP9551537.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          142 a          103 c           86 g          171 t
ORIGIN      
        1 gtcttttcca agtttcgtat tatccttcta taatccagcc atcaattgtg cttgacgtaa
       61 ttctaattgt cgtacatcgc gcggtaggaa ccgacgaatt tcatcttcat taaaaccaac
      121 ttgcagacgt ttatcatcaa tcatgattgg acggcgaagc aatccaggat tgttttgcac
      181 aagttccaat aattcttgta acggcagatc atctaaatcc atgttcagtt tttggaatac
      241 tttggaacgt gtcgagatga tttcttctgt tccgtcttct gtcatttgta aaatcgcttt
      301 taattcagaa ctgttcaatg gttctgaaaa aatattacgt tcaacaaatg gaatctgatg
      361 ttcttgcaac cacgcacgag cctttctgca ggaagtacaa cttggggaag tatataatgt
      421 taacaacgtg caccactcct tttcgtgata agaatcactt ttttgtaaca gaagatcata
      481 ataatctccc atctgtttct at
//
LOCUS       DACDSY010000380          498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.395, whole genome shotgun sequence.
ACCESSION   DACDSY010000380 DACDSY010000000
VERSION     DACDSY010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.395"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..216
                     /locus_tag="IWK44_003117"
     CDS             <1..216
                     /locus_tag="IWK44_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9551538.1"
                     /translation="PINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEI
                     GKVLGITRQGATNLKHRLYKKIQKMLV"
     gene            310..>498
                     /locus_tag="IWK44_003118"
     CDS             310..>498
                     /locus_tag="IWK44_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9551539.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          184 a           70 c           84 g          160 t
ORIGIN      
        1 ccgattaatg ttttaaatgt gactctcctt gttgaagata taaaattagc aaaaatcctt
       61 cctttgttga aagaacctga acaaattttt cttatcgaaa aatttatctt tgataaaact
      121 gataaagaaa ttggaaaggt tctggggatt acaagacagg gtgcgacaaa tttaaaacat
      181 cgactttaca aaaaaattca aaaaatgctg gtttaatatc tgcataataa ctaattttgt
      241 atgcttcttt gctcgttctc ctccttccca aagaaataga ttataagtcc atgggaagaa
      301 ggtagattta tgaattcttt aaaagaagat ttcattttag caaaggctgg aaatgaagaa
      361 gctgttgagg caattctcaa aagatttagc tctttgattc ataagcaatc ctggagaact
      421 ggaaaatatg atcaagattg ttatcaagaa tgtatgcttg caatatatct agctatctcg
      481 aaattcgaaa ttaaagaa
//
LOCUS       DACDSY010000381          485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.396, whole genome shotgun sequence.
ACCESSION   DACDSY010000381 DACDSY010000000
VERSION     DACDSY010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.396"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(62..259)
                     /locus_tag="IWK44_003119"
     CDS             complement(62..259)
                     /locus_tag="IWK44_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9551540.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DACDSY010000382          468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.397, whole genome shotgun sequence.
ACCESSION   DACDSY010000382 DACDSY010000000
VERSION     DACDSY010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.397"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            299..457
                     /locus_tag="IWK44_003120"
     CDS             299..457
                     /locus_tag="IWK44_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9551541.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
BASE COUNT          169 a           73 c           80 g          146 t
ORIGIN      
        1 gtgaatagga aaaaaactat ttttgatcat tgacaattaa atacaatttt tgaataacga
       61 tccctgctat tcgagctaga aactcaagta cgccatgacc tgattagcga caaatactta
      121 gagatttctt tttgttctgg gaaaacaaaa tgccatgaca atagcagcaa ataatgatac
      181 ttccgtaagg ctcgccgtaa actggggaga ggtgaaattc ctatgtgctt acccaaagca
      241 attcaaaaat ttcataacta ctattttagg agatgatttt atagaatcca gagtctacct
      301 gaataaaaaa caaacctgtg catttctagg aatcgataat aatactctag ataagtggat
      361 tcgtatatat aatttcccag taataagagt tggtggtgta gttcgctttg acgtagaaga
      421 tattaaagac tttatgaata catttaaaat aacttgaaat tttcttct
//
LOCUS       DACDSY010000383          465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.398, whole genome shotgun sequence.
ACCESSION   DACDSY010000383 DACDSY010000000
VERSION     DACDSY010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.398"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>465)
                     /locus_tag="IWK44_003121"
     CDS             complement(<1..>465)
                     /locus_tag="IWK44_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP9551542.1"
                     /translation="FSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQ
                     HSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNEL
                     KRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLG"
BASE COUNT          164 a           84 c           59 g          158 t
ORIGIN      
        1 taccaagaaa cgcataattc actttacttc taaaaaatgt tttgagtaaa ctaaagatat
       61 ttaacacata gaaagcatag atacatataa aagcaattat accacttgat acaagtactt
      121 gaagaaatcc attatgcatg tttccatgtg ctctttttag ttcattcaca tctgtagtat
      181 caagatctac ttttttcaaa aattttgcat cgctattccc atagaaatcg gtctctccat
      241 aaccaaataa caaagttggt cttataactt taagcgcagc tttccaaatc gtaaaacgac
      301 cagctgaaat ttcggagtta tcactagaat gctgtaatat tacttctttt tctggaagtg
      361 gagttttatc tgattcattt gactctataa cttctacttt tctatcaatc gatacaggaa
      421 ttgttgctaa aaaatcctta gcataattag taccaaaaga aaaat
//
LOCUS       DACDSY010000384          462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.399, whole genome shotgun sequence.
ACCESSION   DACDSY010000384 DACDSY010000000
VERSION     DACDSY010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.399"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..430)
                     /locus_tag="IWK44_003122"
     CDS             complement(<1..430)
                     /locus_tag="IWK44_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9551543.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DACDSY010000385          455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.400, whole genome shotgun sequence.
ACCESSION   DACDSY010000385 DACDSY010000000
VERSION     DACDSY010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.400"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>455
                     /locus_tag="IWK44_003123"
     CDS             <1..>455
                     /locus_tag="IWK44_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551544.1"
                     /translation="GTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADD
                     TEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKV
                     DVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKV"
BASE COUNT          186 a           68 c          106 g           95 t
ORIGIN      
        1 ggaacaacag tctttgcagt gaaagacggc gtaagcttac cagaagataa acttccagtg
       61 ttgaaagcaa aagatggtta tacagatgcg aaatggccag aggaagcaac tcaaccaatt
      121 acagcagatg atacagaatt tgtatcaagt gcaacaaaac tggatgataa atctgacgct
      181 gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga accagacgca
      241 tttgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac ttggaaagaa
      301 aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac atatccagat
      361 ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc tgacgccgat
      421 aaatatgagc caacagtaga aggcgaaaaa gtaga
//
LOCUS       DACDSY010000386          454 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.401, whole genome shotgun sequence.
ACCESSION   DACDSY010000386 DACDSY010000000
VERSION     DACDSY010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.401"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            213..>454
                     /locus_tag="IWK44_003124"
     CDS             213..>454
                     /locus_tag="IWK44_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551545.1"
                     /translation="MYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILP
                     LILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVA"
BASE COUNT          132 a           73 c           64 g          185 t
ORIGIN      
        1 acattaatcg tgcgttaagt ttagaatcta tttttagctc ccctattaat tttgaaacgt
       61 ttagatcata tggcatgcaa gtaaataatt tttacccatg gttaactcta tatcctttat
      121 ttttattgat caagtttact aatttagcaa taggctataa cctttttctt tatattgtta
      181 cgctcattac tctatttata tgtcattatg taatgtatga aattaccaaa aaacatgtta
      241 ctagctcctt tttcgctatt atatatacaa catcttcttt taggagtgta gaaatatttc
      301 ttcgaggggc aatgggggaa ttactcgcaa tgtcaatact tcctcttatt ttactcgggt
      361 ttataaagct ttacgactca aaaaaagaat cttgggttat gttggcgatt agcatgactt
      421 tattaattta cactcatgtt ctttctgtag ctat
//
LOCUS       DACDSY010000387          447 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.402, whole genome shotgun sequence.
ACCESSION   DACDSY010000387 DACDSY010000000
VERSION     DACDSY010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.402"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>447
                     /locus_tag="IWK44_003125"
     CDS             <1..>447
                     /locus_tag="IWK44_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9551546.1"
                     /translation="VTHNKKISYQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFA
                     IELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAK
                     RNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNYS"
BASE COUNT          156 a           56 c           83 g          152 t
ORIGIN      
        1 gtgacgcata ataaaaaaat ttcttatcaa taccatgtag gacagtattt acccgactgg
       61 catccttggg aagattatcg caattttttt gtttcaaaca aaaaaactaa tgggtgtaga
      121 gaaatattcg ctattgaatt accttggatt atagatacat ttgggaaagt taaatcgttt
      181 actgtaatga cccagaaatt atccaatctt gatattgatt attcggacag tttcactctt
      241 attattgagc atgaaactgg tatagttgga actttaagtg tagatgttgt ttcaagagta
      301 gctaaaagaa atttaactat aattggtgaa aagattttaa tagaatggga aggtaacccg
      361 gattctctat tttcatggaa tgaagaaact tctagtatga cagatattca attgtacgat
      421 gaggttatgc atgataataa ttattca
//
LOCUS       DACDSY010000388          445 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.403, whole genome shotgun sequence.
ACCESSION   DACDSY010000388 DACDSY010000000
VERSION     DACDSY010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.403"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            78..287
                     /locus_tag="IWK44_003126"
     CDS             78..287
                     /locus_tag="IWK44_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9551547.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            280..429
                     /locus_tag="IWK44_003127"
     CDS             280..429
                     /locus_tag="IWK44_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP9551548.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
BASE COUNT          183 a           40 c           97 g          125 t
ORIGIN      
        1 tgaataaaaa tatgcaggaa cgctactatt tttagtagtg ttctttttta tatttgaata
       61 aaaaatgtgg tggttaaatg gtggttataa caaaaaatga attaatgaaa ttaggttatg
      121 ggaagtatca gtctgagaga atcatacgac aggcaaaagc aattatggtt tcaaaaagct
      181 atacctatta cgaaaataga agattaggac tagtacctgt tgaatctgta gaagaagttt
      241 taggtatgaa gatagaggta gaggaggatt ttcagaatca tggttaattt taaaaaagat
      301 aaaaatggga aatatcattt tactgctagt ttgggattcg acaaacgaac agggaaaaga
      361 gtacaaaaaa acgtagaggg gttaaaacag ttaaagaaga aaaaaagcgt ataccgaact
      421 tgtttctaat tatggaaaag aagca
//
LOCUS       DACDSY010000389          441 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.404, whole genome shotgun sequence.
ACCESSION   DACDSY010000389 DACDSY010000000
VERSION     DACDSY010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.404"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            19..>441
                     /locus_tag="IWK44_003128"
     CDS             19..>441
                     /locus_tag="IWK44_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9551549.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVL"
BASE COUNT          163 a           81 c           73 g          124 t
ORIGIN      
        1 agaaaagagg acttccctat gaatgatttt actacagaaa ttgtgcaaac tctagtcact
       61 aaaggcgatt taaatgaatt attccgttcg cacttagaaa aagcgataaa cacactccta
      121 cggactgaat taacggcttt tttagattac gaaaaatatg atcgcactgg ttttaattca
      181 ggtaattcga gaaacggttc ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca
      241 ttggaaatac ctagagatcg taatggtgag tttaaacaac aaactttacc agcctacaaa
      301 agaacaaacg atacattgga aaccactatt atccatttat tcgaaaaagg tgttacgatg
      361 tctgaaattg ctgatttgat cgaaaaaatg tacggtcatc actatactcc acaaaccatg
      421 tccaacatga ctaaagttct g
//
LOCUS       DACDSY010000390          441 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.405, whole genome shotgun sequence.
ACCESSION   DACDSY010000390 DACDSY010000000
VERSION     DACDSY010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.405"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..407
                     /locus_tag="IWK44_003129"
     CDS             <1..407
                     /locus_tag="IWK44_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551550.1"
                     /translation="DIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVL
                     LLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIV
                     LFCEHYNRLSEEKILLPENWAELRKLVEYGII"
BASE COUNT          163 a           66 c           64 g          148 t
ORIGIN      
        1 cagatatttt cgctaaagta aatagtatat tttctggaaa aaaagaaact ttaattaagc
       61 ttcatcctag agcaaggaca aatcgttatg gtgaaaaaaa catattgaat tccagtttac
      121 cgtttgaagt cttgctactt gaaaatgact tctcttcaac acttttgatt agtcctatat
      181 ctacaatttc tttttcacca tctatgatgt ttgaaaagac gatcaactct attatgttgg
      241 ctaaattaat tttaaatgaa tatacagcat tatctgaaga taaaaaagaa acattgacaa
      301 atattgttct tttctgtgaa cattataacc gtctatcaga agaaaaaatt ttactcccag
      361 aaaactgggc agaattaaga aagttggtag agtatgggat tatttaacac actttccaag
      421 tcaaaattag ttaagtccag t
//
LOCUS       DACDSY010000391          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.406, whole genome shotgun sequence.
ACCESSION   DACDSY010000391 DACDSY010000000
VERSION     DACDSY010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.406"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..339)
                     /locus_tag="IWK44_003130"
     CDS             complement(<1..339)
                     /locus_tag="IWK44_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9551551.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DACDSY010000392          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.407, whole genome shotgun sequence.
ACCESSION   DACDSY010000392 DACDSY010000000
VERSION     DACDSY010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.407"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..>415)
                     /locus_tag="IWK44_003131"
     CDS             complement(1..>415)
                     /locus_tag="IWK44_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP9551552.1"
                     /translation="KYAGKPIIIVNTATKCGLAPQFKQLEELYQAYQTQGLVILGFPS
                     NQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTDPLFTYLKNEAPGTVTKAIK
                     WNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          119 a           65 c           83 g          148 t
ORIGIN      
        1 ttaaagaacg gattcaattg cagcggtcat tttcaatgga gaagtttgtg gtgcaaatcg
       61 ttcgattact ttgccatttc tatcaatcaa aaatttagta aaattccatt ttattgcttt
      121 agtgacagta ccaggagctt catttttaag ataagtaaaa agaggatctg tattttctcc
      181 atttacgtca atcaattgat gcatagggaa ggtaacgcca taagtggttt tacaagcttc
      241 tgctgcttca gtcgctgaat ctagctcttg tttaaattga ttggaaggaa agcctaagat
      301 aaccagacct tgtgtctgat aagcttggta aagttcttct aattgtttaa attgaggtgc
      361 tagaccgcat tttgttgcag tgttaacaat gattatcggt ttgcctgcat atttt
//
LOCUS       DACDSY010000393          410 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.408, whole genome shotgun sequence.
ACCESSION   DACDSY010000393 DACDSY010000000
VERSION     DACDSY010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.408"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            36..299
                     /locus_tag="IWK44_003132"
     CDS             36..299
                     /locus_tag="IWK44_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HAP9551553.1"
                     /translation="MNKNTSLHEESLIIIEALGGKENIESVEACATRLRVAVTNGEII
                     QKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            312..>410
                     /locus_tag="IWK44_003133"
     CDS             312..>410
                     /locus_tag="IWK44_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9551554.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFS"
BASE COUNT          152 a           56 c           81 g          121 t
ORIGIN      
        1 tggaagaaga atcagaagtt cattctgaaa aagtaataaa caaaaataca tcattacatg
       61 aagagtctct tatcattatt gaagctcttg gtggaaaaga aaatattgag tctgttgaag
      121 cttgtgcaac tcgtttgcgt gttgctgtta cgaatgggga gattattcaa aaacaaacaa
      181 taaaagacac tggtgcaact gctgtcttcg aagtgaaggg tggcatccaa gctgtttttg
      241 gagggaaagc tgacttatta agtcaagaaa tcaatcaaat attaggaaaa gataattagg
      301 aggtttttaa tatgtttgaa attttcaaaa agaaaaaaca agaaactttt tatgcacctt
      361 gttccggaaa aatcatttcc atcacagaag taaatgatcc tgtcttttca
//
LOCUS       DACDSY010000394          409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.409, whole genome shotgun sequence.
ACCESSION   DACDSY010000394 DACDSY010000000
VERSION     DACDSY010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.409"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..379
                     /locus_tag="IWK44_003134"
     CDS             <1..379
                     /locus_tag="IWK44_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP9551555.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          139 a           79 c           81 g          110 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattctt
//
LOCUS       DACDSY010000395          409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.410, whole genome shotgun sequence.
ACCESSION   DACDSY010000395 DACDSY010000000
VERSION     DACDSY010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.410"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>409
                     /locus_tag="IWK44_003135"
     CDS             <1..>409
                     /locus_tag="IWK44_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9551556.1"
                     /translation="YKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETYSQVL
                     KRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRILKSL
                     FDLKLAKATIMGIERDSYYWEEIGLYAPQIEGE"
BASE COUNT          161 a           46 c           77 g          125 t
ORIGIN      
        1 tttataaagg tgattttcat tttcaaaatg ctgagactat tgaacgagaa catcattatt
       61 tcgatttatt agtgaataca gtggtagaat tagcgaaatg ggcaaagtca aacgaaactt
      121 atagtcaagt acttaaaaga attatgagtc aagaaacttc atttatctat gagttaagca
      181 agtcaaattt gaaatatact tataatgaag atgaacttga aattttagat aatccagaaa
      241 attcacaatg gattaaagag ttgaagagag aagactttat tgagacacta agaattctaa
      301 aatcgttatt tgatttgaaa ttagccaaag ctaccatcat gggaattgaa agagattcat
      361 attattggga ggaaattgga ctatatgctc cccagattga aggtgaaga
//
LOCUS       DACDSY010000396          399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.411, whole genome shotgun sequence.
ACCESSION   DACDSY010000396 DACDSY010000000
VERSION     DACDSY010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.411"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>399)
                     /locus_tag="IWK44_003136"
     CDS             complement(<1..>399)
                     /locus_tag="IWK44_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9551557.1"
                     /translation="SYLLEIFLEILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRV
                     KFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNV
                     DFVSLDETPQEWAKKVIKEPYANKITQELK"
BASE COUNT          118 a           75 c           46 g          160 t
ORIGIN      
        1 ctctttagtt cttgtgttat tttattagca tacggctctt ttattacttt ttttgcccat
       61 tcttgtggtg tttcatctaa tgatacaaaa tccacattat ttgttaactt tgtatctttg
      121 gaaatagtat ctgctaagac taattttaat tttgctgctt gtgcttctaa tccaaccatt
      181 ggcagacctt cataaagaga aggcattaca aaaacatcca tcacagataa taactcggga
      241 atatcgtttc tttctcctaa aaattttact cttttctcta tatccaattc tactgctttg
      301 gctttcagtt tatttctaag tgacccttca cctactaaaa tcaagtaaga atcagaattc
      361 atttttagta tttctaaaaa aatctctaac aaataagat
//
LOCUS       DACDSY010000397          376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.412, whole genome shotgun sequence.
ACCESSION   DACDSY010000397 DACDSY010000000
VERSION     DACDSY010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.412"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          123 a           62 c           79 g          112 t
ORIGIN      
        1 agtaactgct cctcaactga aatcaattaa agaactagaa tttgagaatg cactggtaat
       61 ttgtatgaca tgtactgatg aagtatttcg aaaacatttt taaaggttga gatgtatata
      121 gatttaacca atcatttatc tctctcggaa aaggaatatt aactaaccag cgggttatca
      181 aaaataggta ggaaaactgt acttaatggc ttatttcgcg ctgattatga gaatatcagt
      241 ctgtttgatt aatttttagg gttcaaaata cagagacaaa ctccaggtcg ggtttcgcca
      301 caagcgaatt acccgatctg gagttttgtc tattgcagga aggcgtagcc ggactgcagg
      361 aataaagccc ccggtt
//
LOCUS       DACDSY010000398          373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.denovo.414, whole genome shotgun sequence.
ACCESSION   DACDSY010000398 DACDSY010000000
VERSION     DACDSY010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.denovo.414"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            13..312
                     /locus_tag="IWK44_003137"
     CDS             13..312
                     /locus_tag="IWK44_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9551558.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          144 a           50 c           76 g          103 t
ORIGIN      
        1 gaggaaatcg tgatgtctaa aagtgaagaa atagaaaaca tggtactaaa attatatgat
       61 ctcgttttaa atccagagat caaagaaaag gaacgtatac ttttgataga cgcaaagaca
      121 ggcttagaaa aaggtcagta ttatcctaaa gtcataaata atttggaacg tagtttacgt
      181 ccgttagcaa tcaggggaga attgtctaaa ccagtatcac cgttttatat ggagatatca
      241 acaataggaa aatttgaaaa agaattaggt cgaggaatgg cttcggcacc gattactttt
      301 gggcatttat aaaaacgagt tattcatctc aatgaattaa aaagtatagt caatctcata
      361 atgcaggcta ggt
//
LOCUS       DACDSY010000399        14231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.guided.072, whole genome shotgun sequence.
ACCESSION   DACDSY010000399 DACDSY010000000
VERSION     DACDSY010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.guided.072"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..500
                     /locus_tag="IWK44_003138"
     CDS             <1..500
                     /locus_tag="IWK44_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9551559.1"
                     /translation="KTDLFFGATNNGRKERAESYKQEFIKRDVTTNYFILPHRGNEQP
                     GYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQKKLVTDNQAIAHYHFYRP
                     ENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKRFITNDSTIYEQYEYDLS
                     SVENK"
     gene            complement(650..1591)
                     /locus_tag="IWK44_003139"
     CDS             complement(650..1591)
                     /locus_tag="IWK44_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9551560.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1717..2838
                     /locus_tag="IWK44_003140"
     CDS             1717..2838
                     /locus_tag="IWK44_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9551561.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3096..4598
                     /locus_tag="IWK44_003141"
     CDS             3096..4598
                     /locus_tag="IWK44_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9551562.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4665..5192
                     /locus_tag="IWK44_003142"
     CDS             4665..5192
                     /locus_tag="IWK44_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9551563.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5403..6464
                     /locus_tag="IWK44_003143"
     CDS             5403..6464
                     /locus_tag="IWK44_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9551564.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6467..7363
                     /gene="murQ"
                     /locus_tag="IWK44_003144"
     CDS             6467..7363
                     /gene="murQ"
                     /locus_tag="IWK44_003144"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9551565.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7377..9359
                     /locus_tag="IWK44_003145"
     CDS             7377..9359
                     /locus_tag="IWK44_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9551566.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9424..9870)
                     /gene="nrdI"
                     /locus_tag="IWK44_003146"
     CDS             complement(9424..9870)
                     /gene="nrdI"
                     /locus_tag="IWK44_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP9551567.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10081..11505
                     /locus_tag="IWK44_003147"
     CDS             10081..11505
                     /locus_tag="IWK44_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9551568.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(11686..12459)
                     /locus_tag="IWK44_003148"
     CDS             complement(11686..12459)
                     /locus_tag="IWK44_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9551569.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12456..13361)
                     /locus_tag="IWK44_003149"
     CDS             complement(12456..13361)
                     /locus_tag="IWK44_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9551570.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(13515..13835)
                     /locus_tag="IWK44_003150"
     CDS             complement(13515..13835)
                     /locus_tag="IWK44_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP9551571.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4555 a         2538 c         3010 g         4128 t
ORIGIN      
        1 caaagacgga tctatttttt ggtgcaacga ataatgggag aaaagaacga gcagaaagct
       61 ataagcagga atttataaaa agagatgtta cgaccaacta tttcattctt ccacatcgag
      121 ggaatgaaca acctggatat cttacttatg aagagtacct gaaaaaaaca ggggaatcgc
      181 gcgggattct ggaactgttg cgggagggtc agcaaggagt gactttgcgg acatttgaat
      241 ccatgtattt tcaaaagaaa ctagtgacgg ataatcaagc gattgctcat tatcattttt
      301 atcgtcctga aaatattttt ttgcttcgag aacgtgattt agatgaatta ccggaatttc
      361 tgcatacacc atttaagcca atcctatcag agctattgga attttatgat gcagaaaatt
      421 gggcaaaacg atttatcact aacgattcca ctatttatga gcaatacgaa tatgacctct
      481 cctctgttga aaacaaataa aaaacctaca ttttgaagag attatgtact gaaacaaaca
      541 agcacaattc ttatggcatc atgagagttg tgttttcttt ttgatctttt attgggctta
      601 aaaaaagaga accagctgct gattcagctg attctcatca gagagccttt tattctgcgc
      661 caaggattcc ttcttttgca tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa
      721 tatcatattt ttgtcctttt cgccaaatgg caccattgat gtagtcgatt tcagtcaaac
      781 gatggttttt gatcaggtct tggtacatag atgggtaatg caacccgatg ttgtcaggat
      841 cataagtaga agcaatatgt tggtacactt cttcttgatc tagtatgatt ccttcttttg
      901 ctgcgataga agcaaattca gatacgattg ttcgaaccat gctctctgcc gctttttgcg
      961 cgcctagttc agcaatatta caatccaaaa tcgtgcataa accattcaat gttccattta
     1021 cacaagcttt tcgccaaatg gaataacgaa cgttatggct atagatcgga tttaaatttg
     1081 cttcttgaaa gacttctaca actttttttg taaatgcttc gccttctggt tccaagttct
     1141 ctagttcgat ttctccatca cctaacaatg tgacttttcc gggaccagct agaccagctg
     1201 tccacatcgt gatcccaaat aagatatttt tctttggcac atatttttcc agtacatctt
     1261 catgacctaa tccatttagt aaacacaaaa cataagtatt gtcagtaata atggattgga
     1321 tcgaacaaaa catatcatct aattgattgg cttttgttaa tgctacgatc aagtccacgt
     1381 gttcgttgct ttcgatgatt tcttctggtg aatagattgg cagttttgcg accacttctt
     1441 taccgttaaa gtcagcaatc aacccatttg tacgaatggc ttcaatgtga gcaggccaac
     1501 gatcgatcaa gaggacctca tttccgcttt gatgtagcat caatccgatt cggctgccca
     1561 ttgctccggc cccagctatt gcgattttca tgttttttct ctcctttatt caatgagttc
     1621 ccttttagta tatagaaaaa tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa
     1681 aaagtgtctt acttaagaag aaagtgaggg atacagatgg atattttggc ggcaattgat
     1741 tcatttaaag gaagtgcttc ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa
     1801 gatcgtaaag gaagaaaaat caatgtgccg attgcagatg gcggagaaaa tactgcagaa
     1861 gcaatctatt acgcgctcag gggaacatgg cgtaccattg atgggatgga tctcttattc
     1921 cgtcagcgaa aaatcagcta tttattgaca acttggcaag gaaaaaacac cgcagtcatc
     1981 gaatctgcac aagcattggg gttagatcta cttgataaac caacggatca aactatccga
     2041 cttgcttcca gttatgcctt aggagaactt attaaagatg cactgcttca agaagtagaa
     2101 caaattattg tcactttagg aggaagcggg tgtagcgatg gtggtctagg attactccaa
     2161 tccttgggtg cttctttaga agggtacaca gaaggaaatc ctttattatc tactaaaaaa
     2221 cttcattttg atcgaagaaa cgttcttgtt tctaaagtaa aattgattat cgctgcagat
     2281 gttaccaatc catatacagg tcaacaagga gctgcttggc aatttggaag acaaaaagga
     2341 ggaatagata cgacgctagc tttcttagat aaacaagcag gaaagattgc tcagcaaata
     2401 aaagatttct gggaggtgga tttagatgat attccaggaa gtggagcagc aggcggtatt
     2461 ggtggagccc tttttttgtt gggagcgaag atggtttctg gtttcgattt agtcagccaa
     2521 atcactggat tggaaaaaaa gatcgaacaa gcagatgtca tcattacagg agaagggcgg
     2581 atcgatcagc aaacgatcca cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa
     2641 tatcgaaaac caataatcgc tatttgcgga agtagagacc aagagctaga aggactttct
     2701 gattttctcc caattgtctt aagtattcat ttgggtccaa taggtttaga agaagcaatg
     2761 gaacacaggc aaacattaga aaaaacaaaa atcttaggac agacacttag cagatttttc
     2821 accatcctcc cgaagtagtt gacaaaatca ttttctttat gctatcgtaa tttcaaatat
     2881 aggacaaagt gagggatccg tgcattgaaa aattaagtcg attgatgaaa gctgcattca
     2941 gactgtacct tgtggagaca aggaagcagt gtgcagattt tcgggattgg cttggttttt
     3001 tcgatgtatg ggtctttttg tgtgtatcca acccttgcat cctctttagc cagttctgaa
     3061 aataggactg gtcttttttt acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc
     3121 tgacattcgg ctacgacagc caaggcacat tattatttga acaagcaaat ctaaattttg
     3181 acacacaatg gaaactagga cttatcggac gaaacggtcg aggaaagaca actttactga
     3241 atattctaca aaacaaacta ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct
     3301 atttcccgca acagacaaaa gataaagaac gtttaaccta ttacgtgtta aatgatatta
     3361 cggattttga gatatgggaa atcgaaagag agctccaatt gatgcaaaca gatcctgaaa
     3421 tcttatggag agaattcagc acactatcgg ggggagagaa gacaaaagtc ctactggcac
     3481 ttttatttgt ggatgacact catttcccgt taatcgatga accaacgaat catttggata
     3541 tctctggtag aaaacaagta gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg
     3601 tcagccatga ccggggattt atcgatgaag tagtggacca tgttttagca atcgaaaaaa
     3661 gtcaactgga actttatcaa gggaatttct ctatctatga agaacagaaa aaacttcgtg
     3721 atgaatttga aatggctcaa aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa
     3781 cagcagctga aaaagccgaa tggtctcgtt cccgagaagg agataaaaca aagaaacaag
     3841 tcggattcat cgatactgaa tctagacgag tgaataaagg agcagtgggt gctgatgctg
     3901 cacggacgat gaaacgatcc aaagcaatcg tgaatcggat ggagacccag atcagcgaga
     3961 aagaaaaact attaaaagat atcgaatata tcgattcttt gacgatgaat agccaagcgt
     4021 ctcaccataa gcgactttta agcgtagaag atcttcaatt agggtatgaa aatctgttat
     4081 tcgagccaat tcattttaca atcgagcctc atcagcgggt ggcgatttca ggtcctaacg
     4141 gtgcaggaaa gtcatccatt atccattatc ttctgggggc attcaacggc aaggttatag
     4201 gagaaaaata ccagccaaaa catctgagca ttagttatgc aagccaaaat tatgaagaca
     4261 atcgaggaac gttggcggaa tttgcagaga aaaaccaagt agactaccaa gcatttttga
     4321 acaacctccg aaagcttggg atggaaagag atgtttttca taacaagatc gagcagatga
     4381 gtatgggcca acggaaaaaa gtggaattgg ctaaatcttt atcacagcca gctgaactat
     4441 atacatggga tgaaccattg aattatttgg atgtcttcaa tcaagaacaa ttagaacaac
     4501 tgatcttgaa cgtgaaacct gccatgttac tagtggaaca tgatcaaacc ttcctggata
     4561 aagtatctac tgagattatt tctcttgaga gaatctaacc cttgtaatct caagaacaac
     4621 aaggtatggt taaggggaac tttgccaacg aaggagagaa tcacatgctt tatgtaacga
     4681 gacacggaga aacaacttgg aacgcacaag gattagtttg cggacgggca gatgtgccgc
     4741 tgactgaaaa aggacagatg caggcacaga aactagcaga aaaggtagta gatttgcctg
     4801 ttccaatcac gaaaatcatc cattcccctc ttcaacgagc aagagataca gcacaagcag
     4861 ttgctgatcg tctcagcttg ccgctgaccg ttgatgaacg attggtagaa atggattttg
     4921 gggattatga tggactgcca agcaaagatg agaactttca aaaagcacgt ttagcgttcg
     4981 ctgtacgatt cccaaatggg gagtcagtct tagatgtgta tgcacggatc gttcctttat
     5041 tgaaagaatg catcgaagac gaagaaaatg tctatctgtt agtttgccat aatgccttga
     5101 taagagtgat caatgcttat tttcatccga tgcctaatga ggggttcttt acattcatgg
     5161 tagataatac agaattggtt tcttatgagt aattatgttt ttgtgttatt taaataaaat
     5221 atttcaaggc actgcttttg cttagcagtg ccttttttag gttatgacaa ttcttttgat
     5281 aatataaact caacctttag aaatgaaatt taattatttt aataaaagta ttgaaatttt
     5341 gtttctttag atatatactc tagtcataga aatgaagacg gatacagaaa aagagggata
     5401 atatgtttgg cttttcaata tttatgaacg attctttgtc agaagagaag aaacagtata
     5461 tccgccaaat ggcatccaat ggctttgtcg gtattttcac ttccatgcat ataccagaag
     5521 atgatgtttc ggcatatcaa aagcgattga tcgaactagg aagcgaagcg aaaaagaata
     5581 aactagaatt gatggtcgat atttcaggag aggcattgga acgagcgggt ttttcattag
     5641 aagatctaac gcctttgaaa gcaattggtg taacaggttt aagaatggat tatcatattt
     5701 ccaatcagca gattgccgac tggtcacatc aattgaaaat cagtttgaat gctagcacga
     5761 tcacgcctaa agatatcgat gaattgaaag aagcagaagc tgatttctca cagattgaag
     5821 cttggcataa ttattatcca cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa
     5881 aaaatcaatg gctcaaaatt caaggtctcc ttgtccaagg ttttgtacca ggagatacag
     5941 agttgagagg cccactatat caaggattgc caactttgga agaacatcga gggatccatc
     6001 ctcttgcagg cgctctagat ctattagcaa gtaacacgga tattgtttac atcggagatg
     6061 caggtctgtc agaaaacgtt caggagcaat ttgcttcttt ccaaaaagaa cagacagtct
     6121 tgctccatac ggaacctgta gatgaagaat tttacgaata tatattaggg aagcatacga
     6181 atcggcaaga cgatgcaaga gatgtcatac gaagcgtaga tgcacgtttt cgagagattc
     6241 cgcctatccc agcaagaaat acagctacgc gtatgaaagg gtctattact ttagataacg
     6301 aaaagtattt gcgctatatg ggtgagatcc aattaaccaa atatgatttg ccagcggatg
     6361 aaaaagtgaa tgtagtagct aaagttatca aagaagagct accattgatc aaccaaatca
     6421 aagctggtat gaactatcaa tttataagaa aagaaggaag ataaaaatga gcaaaatcaa
     6481 tttagagaac ctaacaacgg aacgcagaaa ccaagaaact tttggattag atgagatgag
     6541 tatttctcaa gccgttcaac taatgaacaa agaagatcag aaagtggccg ctgcggttga
     6601 aaaagaactg acaagtatcg aaccagtgat tgctaacacg attgagtcct ttaaaaaagg
     6661 tggacgtctg atttatatgg gagccggaac aagtggaaga ctaggtgtgt tagatgctgc
     6721 tgaatgcgta ccgacattcg gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg
     6781 agaagaagca atgactgtag ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga
     6841 agatctaatc aagctgaatt tgacaaaaaa tgatatagtt attggtatcg cagctagcgg
     6901 tcgtacacca tatgtgatcg gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg
     6961 aacgatttct tgcaataaac aagccgaaat cagtaaacac gcggatttcc caatcgaagt
     7021 cgatgcaggt ccagaatttt tgacaggctc tactcgattg aagtcaggaa cagcacaaaa
     7081 acttatcttg aatatgattt ctactatcag tatgattggt ataggtaaag tattcaataa
     7141 cttgatggtc gatgtcaaac caacaaatga aaaactaatc gaacgctcaa aacgaatcat
     7201 catgcaagcg acagaagctg attacgaaac agctgaaaag tactttgaag aagcagatca
     7261 aaatgttaag ctggctatcg tcatgatttt gacaaatagt gataaagagg aagctcaagc
     7321 aaaattagct gaagcaaacg gatttgtaaa agggacctta tgagggggaa aagaccatgg
     7381 caaaaaacga actgactttg gaagaactgg caaaacaaat ttataaaggg gcaggcggca
     7441 tgggcaatgt agaaagcatt gtccactgca tgactcgtgt gcgtatgaag atcatcaatg
     7501 atgatcttgt aaatgtctct gaattaaaag cgattccagg agtgcttggt gtagtagaag
     7561 atgaacagct tcaagtcatc atcggacctg ggaaagtgaa caaagtagca cagcaaatgg
     7621 ttgatttggc aggcgtgaaa ttaggagaaa aactttcttc tgtaagtaca gctacagcaa
     7681 attcatcagc atctggaaaa gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac
     7741 aaaaatcgaa acaaaaacca tctaaaatca aatcggtatt aaaagacatt tctaatatct
     7801 ttgtgccaat gattcctgca tttgtcggta ctgggatcgt ggcagggatt gccgcagttt
     7861 tagcaaactt agtcacagcc ggaacacttg atgcagcaac atggcaacaa tatatcgatg
     7921 tgatgaacat cttgaaaaac gggatgttta gctacctagt tatctataca ggtatcaatg
     7981 ctgcacaagt ttttggtgca acaccaactc ttggtggagt tatcggggcc gttgtgatgc
     8041 tgacagggat gaatccggaa gcaccactga aaaacttatt tactggtgaa gctcttgcag
     8101 ctggacaagg tgggatccta ggtgtcatct tcgctgtttg gctattgtca atcgttgaga
     8161 aaaaactgca taaagtagta cctgatgcgg tagatatcat cgttacacca actctttcat
     8221 tgattgccat cggtctgatt gaaattttct tcatcatgcc tttagcaggt ttcatttctg
     8281 atggcatggt cggtgggatc aactgggtac ttggcgttgg tggagcattt gccggcttcg
     8341 ttctaggcac attattcttg ccgatggtta tgttcggact gcatcaaatt ttgactccaa
     8401 tccatgtgca aatgattgat gaaactggtc gtacgctatt gcttccaatt cttgcgatgg
     8461 ctggtgcagg acaagtgggt gcagcattgg cattatggat tcgctgcaaa aaagataaag
     8521 aattaacaga aatgatcaaa ggagctttac cagtcgggat cctaggaatc ggcgaaccat
     8581 tgatttacgg agtaacatta cctctaggac gcccatttat tactgcttgt atcggtggag
     8641 gtatcggcgg ggcagtgatc ggaatgatcg gaaatgtcgg ggctatcgct attggacctt
     8701 caggagccgc tttgatccca ttgatctctg acggtaaatg gttaggctac atccttggat
     8761 tattggcagc ttatgctggt ggcttcgttg ctacattctt cttcggtatt cctaaagacc
     8821 gtttagaaaa agaagaatta gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag
     8881 tagtacctca aacttcagct gaaagtgtga ctatcctctc atcagttgct gacggaacag
     8941 ttgtttcttt agataaagca agtgatccag tttttgcaca aaagatgatg ggtgaaggct
     9001 actttgtaga accagaaaac ggacatatct attctccagt tactggggta gtcagctctg
     9061 tcttcccaac gaaacacgct attgggatca caacgccaaa tggtttagaa atcttgcttc
     9121 atatgggaat caacacagtt gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag
     9181 ggcagcaagt gacacctgat acgcttgtag cagaagctga cttagaagcg atcaaagcag
     9241 caggcaaaga aacatccatg atcgtcttgg taaccaatat ggatcgtgta aaaaactttg
     9301 tcttggaaaa aacaggtaaa gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat
     9361 atgatagaaa caatagaaac gaaagaagaa actgaaatct atttttcagc ttcttctttt
     9421 ttattattta ttgttttctg ctaaaatctg ataaattcgt gtcacatcag atggtgttcc
     9481 ccgtaattca taatctgcca agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt
     9541 tagacaatac tgattattga agtttttatt tccgctaccg actacaccaa gacaaagttt
     9601 atgattgtct tcatgttcca gatattcacg catcgtttcg gtcaagattt ctgtatcacc
     9661 gttatctaaa ccatttccgc cgtctaaata agtaggaacg aaagtaaaaa atggttctgt
     9721 ttctttcgta aaatcactat tttcatggat ttcttttaaa gtaacttcag gattgacagg
     9781 atcttctgca tgcatttttt cagcatattc tgctagatgt ttggcaaagg cacgcgtatt
     9841 tcctgaaatc gatatatata aaatgttcat ggatcatcct ccaattcttt ccttttagta
     9901 tagcatcttt tttaagtttt ttggtggtgt aatttggaaa gggactaggc gctttgcttg
     9961 tataatgaga aaaaagccgg tagaattata tttctattaa agtttttatt aattaagtaa
    10021 aaataaaaaa ggtttgttat actttttgat aaaagcggtt ttattttgga ggatgcttac
    10081 atggatacag agcaacagaa aaaaggaatt ggtttaattc cacttgctgc cttagtaatt
    10141 ggttcagcga ttggaggtgg cgtatttggt attatgacgg acatctccgg atcggctggc
    10201 ggcccagcta ttttcagttg ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg
    10261 atcaataata tcaataaaaa acgtccagat ttaaaaggcg ggatctttag ttacgcagaa
    10321 gaaggttttg gccgtttcgc tggttttatc agcggttggg gttactggct cacatgctgg
    10381 ttaggcaacg tagcttttgc aacattgttg atgagcgcat taggatattt cttcccagta
    10441 tttgaaggtg ggcaaaatct ggcatcgatc gtgatgagta cagtcttttt atggggatat
    10501 gcatggctag tgaatcgggg agtcgaaaat gctagtttcg tcaatgctat cgtcacattc
    10561 tgtaaattag tgccattatt tttatttatt gtagtagcta tcagcacgtt ttcgatccaa
    10621 caattcattg ataacttttc tagcgacatt atattgaatg cgaatggaca gccgatcccg
    10681 ttttccagtc aagtattgag ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa
    10741 ggagcctctg tcgtaggttc acgagcaaaa aaacaatcag atgttgggaa agcaacgatt
    10801 ctagggatat tcggcttgat tattatttac gtcttggttt ccatgcttcc atatgggaca
    10861 atgacgatga atcagctgca aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa
    10921 atgatggttg gtaaatgggg agcagtcgtg atcaacggcg gtttgatcat ttctttagtt
    10981 ggtgtttggc tctcatggac gatcttaccg attgaaacaa tgggaaacat ggctgaagac
    11041 ggacttttgc ctaaagcatg gggaaaactc aacaaaaatg gggcaccaac ttatgctatc
    11101 gtattgacga cgttatgtac gaatgtcttt ttactttcat tattattcac tgacagtgct
    11161 tataactttg cttacacact tggtacagca gctattttct tcacgtggct tttcttagga
    11221 ttgtaccaaa tgaaactttc ttatcagcgg aaagaatggg tccaattttt agtagggctt
    11281 ttagcaagtg cctttcaagt atgggctatg ctagtcgtag cctttaatga agtaatgctt
    11341 gttcttgttc ttttcttgcc aggcatttat ttttacctca aagcacgtaa ggagcaaaac
    11401 gagcctgtac aacatatcca cttagataag attttactta taggtgtgac gatggtcgga
    11461 gtgattgcaa tggttttatt tgccactggg atgcttcacg tatagaagta taaaagaagt
    11521 ataaagagag gttgtgagcc tgtcgtttat tcaaaaacat actaggaaag gagctgtgac
    11581 acaaatgttg tcacagctcc tttttcagaa taaacggtgt ttaagagaca gccaatcggc
    11641 aataaggctg aacgtctctt tcacagcctc ttttttgatg agtcatcata cattttttcg
    11701 cttaaatacg agatatccta atgctaagaa caaaacgatg taagctaaat taccgaatag
    11761 catttgatta gtagaaagat gagtcaattg cttcatgatc tcttcgtttc caatttggtt
    11821 ttgcagattc aacatactga tcggattcca tttgatccac tcccattttt ggatgagtgc
    11881 aataaggata ccagatatga ttgacgaagc aaaataaaag acgattcctg ctgagatgga
    11941 tgcgccggaa ctattgataa agcaagcaat catcaacact aaacttaaaa tcagccataa
    12001 accgatataa ctacccaaag tataagtgaa caagctttgg atcagtgttt gaccagtaga
    12061 aaccttctcc gtgaaggaaa tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat
    12121 aatagctaaa aggtataaat aaacagaata aataactaat gtgatccatt tgcttactaa
    12181 tatttctcct cgagaatact ttcgataaag gagatttttc aatgtaccat attgtgcttc
    12241 catcgaaatg atcgtgcttg cagctgcaat catgataaag acaatccaag atgtggcact
    12301 aaaaagctga gcagtcattt caactggatc aatgatcgtc gtgtattttt tggctagaag
    12361 tgctgttcct atgagcaaaa caactaatag aacagataaa acagcagttg actttttctt
    12421 gatcaattta aataattctt gttttacaag caatgtcatt tttcttcttc cccctttact
    12481 gaatccagaa tttccaatag agaagattcc aaatcattcg tcagatgctg gacgtctgtc
    12541 acatggatat tttgtgcaga caatagctgg atcacggttc ctaataattc ttcgttttct
    12601 tttaaaagct tgattttatc aggatcttcc aaagtatatc cagcttctgt caagatcgtt
    12661 cgagcagcta gatcatttgt tgtagagata acgatgaaat cttgcgctgt ttggttcaat
    12721 tcagccattg tacattgttt gatgatcttt ccttgattga tcaggatcag ctcatcgacc
    12781 attttttcaa gctcgctgag taagtgactg gagatcaaga aagaggtgcc ctgagtagcc
    12841 aagtcggtaa tgatttttcg cacatctctt gttgcttgtg gatccaaacc gttcatcggc
    12901 tcatctaaaa taacgataga cggatggttc agtaaggcaa gagcaattcc caatttttgt
    12961 ttcattccca acgagtagga tttcgctttt ttgtgaatgt acttttccat cttgagctga
    13021 ttcacgatag tgtcgatagc agatacatcg ttggtttcag agaataactt cagatgatca
    13081 tagcctgtta agaatggata gatacccggg tattcgatca atgctcctac ttgttccaac
    13141 cctttatgac tagagggaga aatctcattt tcaccaatac agattgttcc atctgagtaa
    13201 tggatcagtc ctaaaatagc tttcatgatc gttgtttttc cagcgccatt aggacctact
    13261 aatccaacga ttttgccagg ggataagcta aaactgacat cgctgagggc ttgtttgtga
    13321 ccaaagttct tggataattc ttggaccgaa agacttttca aaaatattca ctccttttct
    13381 atagatagta caagcataca cctgatgagt ggagaagtcg ctaggtcatt ggacttattt
    13441 cttatttttt cataaaaaaa ttgaatatta aataaaaagg cttatttttt ctgatcagta
    13501 cttattccaa gtgtctagtg gccgctagta atcttgatgc caatgatact gatgagtagc
    13561 atccccacaa agaaccaagt ggaaagattc agcttatcat gaaacaagac cacaccaatt
    13621 atgaccgaac caactgcgcc aatccctgtc cagataggat aagagagact catcggcaaa
    13681 cttttgattg cctttgacaa aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa
    13741 gaataattga ttttagaaaa accatcactc catttcatca tcgttgacca gaatacttcc
    13801 aaaattcctg ctatgattaa ttccatccat gccatactta cattcctcct taactataaa
    13861 aaaatacaca aaaagaggaa aatcatccct tcaaagacct aatggaatga tcatcctctt
    13921 tatcctaccc ggtggcagat tgtatatctt tttctgtagt atagcacacc tatccagata
    13981 tcggcaatct catacttttt agtaaagtgt accaagcact gacggatata tcgttagcgg
    14041 aaaagaagat gaatgctttg caatttcata gaaagaagtt gttatttatt aatcatttaa
    14101 aaaaaacagt aactagacct tatttctata tattatcggg caataaatta aaaaaagctt
    14161 taccttacaa taagtaagat aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat
    14221 agtgtctatt t
//
LOCUS       DACDSY010000400         9376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.guided.112, whole genome shotgun sequence.
ACCESSION   DACDSY010000400 DACDSY010000000
VERSION     DACDSY010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.guided.112"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1801
                     /locus_tag="IWK44_003151"
     CDS             <1..1801
                     /locus_tag="IWK44_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9551572.1"
                     /translation="KDRIGLIGTNGTGKTSLLNIIAGIDSSDGDRQTVFYPTDYRIGY
                     LSQAAEFSDELTVLQAVFQGNSPLIQTVRAYEEALIELGENGEDPDVQKRYAKAEEQM
                     NKEDAWTTDTNAKIILQKLGISELDKKISTLSGGQKKRVSLAQVLIDEPDLLLLDEPT
                     NHLDYEAIEWLENYLKQYRGALLMVTHDRYFLDRVANRIFELSFGKLYEYKGNYEAYV
                     LEKAERDRVEVEQEEKRKRLYKQELAWMRAGVQARGTKQQARINRFEDLKENLYQVNQ
                     EDDLELNLATQRLGKKVLEIKDGSYRINDQTLLEHLDLLIQSRERLGITGKNGAGKST
                     LLNILAGRIPLDSGTMSIGETVHLAYYTQENEEMAPDKRMIAYLQEAAEEAKTADGSQ
                     IGVAELLERFLFPRFMHGTLIGKLSGGEKRRLFLLKLLIQQPNVLLLDEPTNDLDIAT
                     LTILEDYFRSFPGAVITVSHDRYFLDKVADKLLVFQGNGKQELYYGNMSSYLLKQKET
                     QQPAEKAKPKIQSKEPAEKKKLSYMEQKEWETIEDEIAELEEKISLLQEEMNHQGDNF
                     TRLQELQNDVSETEAQLEEKMARWEYLSEWVED"
     gene            1813..2760
                     /locus_tag="IWK44_003152"
     CDS             1813..2760
                     /locus_tag="IWK44_003152"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9551573.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            2780..3307
                     /locus_tag="IWK44_003153"
     CDS             2780..3307
                     /locus_tag="IWK44_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9551574.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3370..4023)
                     /locus_tag="IWK44_003154"
     CDS             complement(3370..4023)
                     /locus_tag="IWK44_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9551575.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            4155..4997
                     /locus_tag="IWK44_003155"
     CDS             4155..4997
                     /locus_tag="IWK44_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9551576.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5155..6051
                     /locus_tag="IWK44_003156"
     CDS             5155..6051
                     /locus_tag="IWK44_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9551577.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            6054..6767
                     /locus_tag="IWK44_003157"
     CDS             6054..6767
                     /locus_tag="IWK44_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9551578.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDALNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            6787..7305
                     /gene="msrA"
                     /locus_tag="IWK44_003158"
     CDS             6787..7305
                     /gene="msrA"
                     /locus_tag="IWK44_003158"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9551579.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            7302..7529
                     /locus_tag="IWK44_003159"
     CDS             7302..7529
                     /locus_tag="IWK44_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP9551580.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            7681..8232
                     /gene="lepB"
                     /locus_tag="IWK44_003160"
     CDS             7681..8232
                     /gene="lepB"
                     /locus_tag="IWK44_003160"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9551581.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(8315..8857)
                     /locus_tag="IWK44_003161"
     CDS             complement(8315..8857)
                     /locus_tag="IWK44_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9551582.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(9094..9348)
                     /locus_tag="IWK44_003162"
     CDS             complement(9094..9348)
                     /locus_tag="IWK44_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9551583.1"
                     /translation="MLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQI
                     AKENGGTFHEVDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         3358 a         1638 c         1863 g         2517 t
ORIGIN      
        1 aaaagaccgt atcgggttga ttgggacgaa cgggacgggt aaaacaagtc tcttaaatat
       61 tattgccggt atcgatagca gcgacggtga tcggcaaaca gtgttttatc caactgatta
      121 ccgtattggt tatctttctc aagcagctga attttccgat gaattaactg ttctccaagc
      181 agtctttcaa ggaaacagcc ccttgatcca aactgttcga gcatatgagg aagcattgat
      241 cgaactaggg gaaaacggag aagaccctga tgtgcaaaaa cgttatgcta aagcagaaga
      301 acaaatgaat aaagaagatg cttggactac agataccaac gcaaagatta ttttgcaaaa
      361 attaggaatc agtgaattag ataaaaaaat cagtacttta tcaggtggtc agaaaaaacg
      421 agttagtctg gcgcaagtgt tgattgatga acccgattta ctattactag acgaaccaac
      481 aaaccattta gattatgaag cgatcgagtg gttggaaaac tatttgaaac aataccgtgg
      541 cgcactattg atggttaccc acgatcgtta ttttctagat agagtagcaa accgaatctt
      601 cgaactttca tttggaaaat tatacgagta taaaggaaat tatgaagctt acgtcttaga
      661 aaaagcagaa cgtgatcgtg tagaagtaga acaagaagaa aagcgcaagc ggctttataa
      721 acaggaatta gcttggatga gggctggcgt acaggcacgt ggcaccaagc agcaagcacg
      781 gatcaaccgt ttcgaagatc taaaggagaa cttgtaccaa gtaaaccaag aagatgattt
      841 agagctgaac ctagcgacac aacgtttagg gaaaaaagta ctggaaatca aagatggcag
      901 ttaccgtata aatgaccaaa cactgctaga acatttagat ttattgattc aatctcggga
      961 aagactaggt attactggta aaaatggtgc gggaaaatcg acattgctta atattctagc
     1021 tggcagaatt cccctggata gcggtacaat gtctatcggt gaaacggttc atcttgcgta
     1081 ttatacacaa gaaaatgaag aaatggcacc agataaacgg atgattgctt acttacaaga
     1141 agctgctgaa gaagcaaaaa cagcagatgg cagtcaaatc ggagtagcag aattacttga
     1201 acgattttta tttccaagat ttatgcatgg gacactgata ggcaaacttt caggcggaga
     1261 aaaacgccgg ttatttttgt tgaaactgct gattcagcaa cctaatgttt tattattgga
     1321 tgaaccgacc aatgatttgg acatagcaac attgacgatt ttggaagatt attttagaag
     1381 ttttccaggt gctgtgatca ctgtttccca tgaccgttat ttcttggaca aggtagcaga
     1441 taagttatta gtattccaag gaaacggaaa acaagaactt tattacggaa acatgtccag
     1501 ttatttactg aagcaaaaag agacccagca gcctgctgaa aaagcgaaac caaaaataca
     1561 gagcaaggaa cctgctgaaa agaagaaatt atcttacatg gaacaaaaag aatgggaaac
     1621 gattgaagat gagattgcag aacttgaaga aaaaatctca ttgctccaag aagagatgaa
     1681 tcatcaagga gataatttca ctcgattaca agaattgcaa aacgacgtat ctgaaacaga
     1741 agcacaactt gaagaaaaaa tggcacggtg ggaatacttg agtgaatggg tagaagatta
     1801 ggaggaaaaa ggatggaaca agcgtatctt gacttaggaa gaaaattgtt ggaagaaggg
     1861 catcgtaaag aagacagaac aggcacaggc acattaagct tattcggata ccaaatgcgg
     1921 tttgatctgc aaaaagggtt tcctttgtta acaacgaaac gagtaccttt tggattgatc
     1981 aaaagcgaat tgctttggtt tttaaaagga gatacgaata tccgctatct attacaaaat
     2041 aaaaaccata tttgggatga atgggcattt gaacgctaca tcaaaagtcc agactataat
     2101 ggaccagata tgactgattt tggccataga tgtttgacag atcctgaatt caatgaagaa
     2161 tataaaaagc agagcaagct tttttgcgag aagatcctaa ccgatgattc ctttgctgaa
     2221 acatacggcg atcttggaca catctacggc tatcaatggc gtcactggga aacaaaagac
     2281 ggtggcttta tcgatcaaat caaacaagtg atcgaagcaa tcaaaaagac tcctgattct
     2341 agaagattga tcgtttctgc atggaatccg gaagatgttc ctagcatggc tttgcctcct
     2401 tgtcatacga tgtttcaatt ttatgttcaa gagggacggc taagctgcca attgtatcaa
     2461 cgcagtgggg atgtgtttct aggcgttccg ttcaacattg caagttatgc gttgctgact
     2521 cacctaattg cacatgaaac aggtttagcg gttggcgaat ttgttcacac attaggagat
     2581 gctcatcttt atatcaatca tatcgaacaa atgaaagaac aattgagccg agaaatacgc
     2641 gcatttccga ctttagtttt gaatcaggaa aagcagtcag tatttgattt tgagatggaa
     2701 gatatccaat tagaaggata tgacccacat ccggcgatca aagctcctat cgctgtttag
     2761 aatcagaaag gatgagaaaa tgttagctgc aatttgggca caggatgaaa acggattgat
     2821 tggaaaagaa gaccgtcttc cttggcgttt gccaaatgat ctgaaattct tcaaacaaat
     2881 gacagaagca aatacactag tgatgggacg aaaaacattc gaagggatgg gtagccgtcc
     2941 gttgccgaat cgtcagacga ttgttttgac gagagattct tcctataaag cagaaggcgt
     3001 gcatgtcatg catagtgtgg aagacgtgct tgctctggaa aaagaaacag acggtatctt
     3061 tttcatcgct ggagggtcag ctgtttatca agaatttctg ccattttgta cgatattata
     3121 ccgtacagtc atccatcatg tattcgatgg agatgcttat tttccacctg ttgactggtc
     3181 tgattggtca ctgatcaata ctagtcaagg agaaatggat gaaaaaaatc gttatccgca
     3241 tcaatttgaa acgtatcagc gtaatgagaa tcctgtaaaa agcgggacag gcgaattgac
     3301 gatataaaaa agctatggat agactagctg ttatttagag ctgtcccatc catagcttta
     3361 ttttttttat taagtaaaga ataaaacaga gaagtacata aaacctgcac ctaacataac
     3421 aaacaaatgc caaacaacat gcatatagcg gatagaattc aagctataaa agaatgcgcc
     3481 tatcgtgtaa gaaagcccgc ctgctaccat caaaataaca ccagtcattc caagagagtg
     3541 ataaagagga acagcagcca cgacacagag ccaccccata aaaatataga tgatcgtaga
     3601 tacttttgat actctctccc gtttatgcaa cgtcaacgct ttatatataa tgccaagaat
     3661 cgcaaacaac cagattaata caaataaagt ccaacctagc catcctcgga tactcagcaa
     3721 acaaaaagga gtataacttc ctgcaatcaa aagaaaaatc gaactatgat cgaatacttg
     3781 gaacaccttt tttgcttttg taaaaatcaa tgaatgaaac aaagtagacg ttaaaaataa
     3841 aagaatcatc atcgatccat agatagcata agagacaata tggagggagg aatgcaagtg
     3901 ggcacctttt actaaaagaa ccactagtcc cgcgatactc aatccagtcc caattccatg
     3961 ggtgactgca ttaaacactt catttaatat ttcatacgtt cgattaaatt tcgttttttc
     4021 catgaaaacg gctcctttac taggtgtaat taatgaaaaa tcattatttg tctgttatac
     4081 taaaaataga aattgtctta catgtagttt aacatgaata aggcatcttg ttaaagaaag
     4141 agagagagaa aataatgaca aatgtaaaaa tcgtaacaga ttcttcatgt acgatggaaa
     4201 aaaatgtacg cgatgcttta ggcattcgtg tcatgccgct atccatcatg atcgacggaa
     4261 cagtatatcc cgatgatgac caactagacg gcgaaagatt tatggaaatg atggcgaaag
     4321 caaaaaatct gcccaaaaca agccaaccac caattggaga attcatctcg atgtatgatg
     4381 agctaggaga agacggcagt gaagtgattt cgatccatat gacaaaaggc ttgagtggaa
     4441 cagtagaagc agcaagacaa gccagtaatc taacaaaaac gaaagtaacg gttatcgaca
     4501 gtgataccac tgaccaagga ttatcttttc aggtaattcg agcagctgaa ttagcaaaag
     4561 aaggtaaaac agtagagaaa atccttgaag aagtcaaaaa agtacaagac aatacaaaat
     4621 tatacatcgg aatatccaca ctagataact tggtaaaagg tggaagaatc agccgaacaa
     4681 ctggtttgct gtctagtgtt ttgaacatac gcgtggtgat gaatttcgcc cattctgaat
     4741 taatccctgt aactaagggg cgaggaaaga aaacgttcga taaatggttt aataatttga
     4801 aaaatgaact gaaagaattg ccaaatgtgc gacaaatcgg tatttcacat gcggatgcaa
     4861 aagaattggc gcaagaattc aaagagggtt tgcaagaatt attcccggac atgcatatcc
     4921 ctgtactgca tacgaatccg gttatcgcta cgcatactgg taaagacgcg tttgcaatca
     4981 tgtattatgt agattagtcg aaaagaggat aagccctcag cctgaaaggc ttgcagctta
     5041 ttctcttttt ctttataaaa ttaggaaaat aattgttttc ttttttcata aagactacct
     5101 ttttgataaa atttttgata acattagaac caatcgaata caagaggtga aaaaatgaaa
     5161 ttaacgaaac gacatttgat cctcgttttg ttcccattat taattgcgat catcatctat
     5221 gcaggtttag ctgcttctat accgaaagct ggcccactaa tgaaaccagt aactactgtg
     5281 agagaagatc caaataaaaa agaaaaactt cactacatag ctatcggaga ttcgctgact
     5341 gaaggagtag gagatcaaac caagcaagga ggattcgttc cccttgtcgc aaatgatcta
     5401 aaagaccgtt ataatttgac cgcggttgaa acagaaaact atggtgtaaa tggtgaacga
     5461 agtgaccaga tcttaaagcg agtaaaaaca aaagaagaaa ttcgaaacaa tatcaaaaca
     5521 gctgatttta tcacactgac tgttggcggt aatgatttga tgaaagttat ccaaaataat
     5581 ttattcgggt taacggtcaa ttcctttaaa aagccactga aaaactatca ggaaaatgtc
     5641 acagaactat tggaagaatt tcgatccctc aataaaaccg cacctattta cattttaggc
     5701 atctacaatc cattttattt gaactttcct gagatcactg acatgcagac aattgttaac
     5761 aattggaaca aaggaacaga aacggtgaca aaaaaaatgc cgaactgcta ctttatccca
     5821 atcaatgatt tgctttatca aggattgaat caggaagtag gtgtcacaga agaaggaaca
     5881 gatcaaacaa ctagcacgac agaaaatcta aatggcatca aaaataatgt gctctatgaa
     5941 gaagatcatt tccatccaaa taatttaggt tatcaattga tggcaaatgc agtgagagat
     6001 cagctgatcg agacacaaaa ccaatggtta gagaaggagg atgatcaata aacatgaaaa
     6061 caagatctga cgtatcttca aacaaaacaa agaaaacaaa ttactggaaa ttcgccttcc
     6121 ttatccttct tggggtagta cttggttcga ccgtattttt aggcagccgt atttttgcta
     6181 atcgggaacc agaactccca gaaataccag ctcttacgga aagacaagga gatcctgtct
     6241 taacgatcaa ttcaaataaa gaaaaagtca atcaaatcat cagctttttc cttagtgagt
     6301 atcaaaaaga tagtgatatt gaatataaat tttatttgga aaatgaagca ctactcaatg
     6361 gtactttcga agtactaggt tttcctatcg atttctattt atattttgat ccgtatgtga
     6421 tggaaaacgg aaatgtccaa ctaaaagcta gaagtctttc tatcggaaca ttgagtttgc
     6481 caatcaaaga tgtgatgaat atgatcaaga gaaactataa attaccagat tggatcgaag
     6541 tcaatacaga tgatctgact atcatgctta ggttggatca attccgaatg caaaacggga
     6601 tgtatattaa agcagataaa atcgacttag taaatgatga cattcggttt agtctatatt
     6661 tgccaaaaga tacctctgat gctttaaaca actcaaatac caacaaaact agcaccagca
     6721 gtactgaaag caagacaaaa gaaaataaaa aatcatcgga tgagtagtat tagaggggga
     6781 atcataatgg aacgagcaat atttgcagga ggctgcttct ggtgtatgat ccagcctttc
     6841 gacacgcttc ctggtatcca tacagtgatg tcaggatata ctggagggca tgtaccagat
     6901 ccgacttatg aacaagtaaa aagccaaacg actggacata cagaagcagt ggagattatc
     6961 tatgatccaa cattgatttc atatgaacaa ttagtagaac tttactggca gcaaactgat
     7021 ccaactgacg ctttcggaca attcgaggat cgcggtgaca attatcgccc ggtgatcttt
     7081 tatttgacag aagaacagcg gatcattgct gaaaaaagta agaaacagtt agctgaaagt
     7141 ggacgtttcg aacagccaat cgttacaaaa atcgaaccag cccaaccatt ttatctagca
     7201 gaagaagacc atcaagagta ttataaaaag catcctgaaa attttaaaag aaaccatgca
     7261 cgccgggctg cctttatcga agaaaattgg aggaacgaag ggtgaatcga agcttttttc
     7321 attatttaat gacactgcga ggcggtggca cacatgatgc tttatctgtt ttcgctacag
     7381 aagccggtaa agacatccaa tttcctaagc acagctctag ctacgaagaa atttctaatt
     7441 atctggaact gaatgtcgac tatttgccgt ccatggatat ctttgacgaa gcatgggaaa
     7501 aatatttaga aaataatcat gacatataag agatagtttt gtactttatg atcaaaaaag
     7561 ttttttgtcc aatagcctaa ttgaaagaat tatttttatt tcagctatct agtcgcattt
     7621 ttttgatatt tttcttataa taaagaaaga atgaaaaaaa gagggatgga ttgatagaga
     7681 ttgacaaaaa aacaacgcta tattaatcgc ttctggcttg tattcaaata cttacttgtt
     7741 tctgtattcc ttgcgttcat gttgcgggga tttttattca tcccggtacc agtggaaggc
     7801 aattcaatgg aaaacgtgtt gaagcaaggt gatatggttg tgatggaaaa attttcggag
     7861 atcagacgat ttgatattgt tgtttttcaa ttagcagatg gcacgattta tatcaaacga
     7921 gtaatcggtc ttccaggaga aaatgtcagc taccaaaatg atcaattgaa gatcaatggg
     7981 aaagtagtta aggaacctta tctaactaaa aacctgaaat cagatcatgc aaatgcatcc
     8041 tatacgacgg actttacact acaagagcta acgggacaat ccaagcttcc ggaagataat
     8101 tattttgtac ttggagataa tcgaagagtt tcaaaagata gtcgttcgtt tggcacgatc
     8161 aataaaactg atattctagg taaagcaaga tttgtctatt atccacttga tgaaataaaa
     8221 tggatccagt gagaaacaat aaagcgtgca gtctgttgag actgcacgct ttattgttta
     8281 atccaaaatc agtttactac cttagtacgt aaaatcaaac agctcgttta aacacttttc
     8341 tagcctctaa agttttgata aatgaataaa caaccattat ttgaataggg atttggatca
     8401 tattcctgat gaatctacct agaaaaatta ctttgaaatc tgcaccaaac aaaacagaca
     8461 tcagccaaga attgataaaa aggctgactg ggatcataat caataattgg gcagcgatga
     8521 cggaaggcag gtttatcttt ttatgatata aaaataagcc attaataaat ccaccaagga
     8581 aagccgtgat aagaaaagca gcttgaaacg ttcctgaacc aaatatcgtg aaatttaaag
     8641 tgttcgacaa catttcaagc aaaccagccc agatcggtcc atatagataa cccataagcg
     8701 ccatacttaa aaatccgaat gtgatttgaa aataagcagg accaatcgct atcttgtata
     8761 agatgacatc aagggctaat aaaagagcag aggtcgacaa caatcttgca ttcatttcgg
     8821 gcaatctaaa aacgtctttt gcaatcaaag tgttcaaaat attctctcct ttgtcagtta
     8881 ataataaaag gataccataa aatcaccaat gtgtcatgac acattggtga tttacataca
     8941 ctggcatcta acaattaaaa aaagggtgag aagactgctt ttataaactg taaaaaagag
     9001 attgtacgaa taaaaaaaat agaggcggag aatacttatc gtatcatccg cctttgtttt
     9061 tttgttataa aaaacgtcag attagttatc tgcttacatc ttcatccata cgggttctaa
     9121 tgtatttccg tctagatctt ggacttctaa tgaaaacatt tcttcttcag gcattcccat
     9181 atctacttcg tggaaagtac caccattttc ttttgcaatt tgagcaaatt tttttacttc
     9241 atccgcgttt tcaacactaa acgaaatcaa agcaccactt gtttttttat tatcagctat
     9301 ttctttatct tttataaatt ttttataaaa atcatgagag agaagcatta tccaaaaagt
     9361 gtcatcccat accatt
//
LOCUS       DACDSY010000401         5985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.guided.152, whole genome shotgun sequence.
ACCESSION   DACDSY010000401 DACDSY010000000
VERSION     DACDSY010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.guided.152"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(40..1026)
                     /locus_tag="IWK44_003163"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="IWK44_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="IWK44_003164"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="IWK44_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP9551584.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="IWK44_003165"
     CDS             1851..3005
                     /locus_tag="IWK44_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9551585.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="IWK44_003166"
     CDS             3220..4188
                     /locus_tag="IWK44_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP9551586.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="IWK44_003167"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="IWK44_003167"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP9551587.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5826
                     /locus_tag="IWK44_003168"
     CDS             5218..5826
                     /locus_tag="IWK44_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP9551588.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1811 a         1161 c         1347 g         1666 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcagtgcc
     5581 attgatctta cgctttatcg attagacacg ggtgagcttg taccaatggg gagccgattt
     5641 gattttatgg atgaacgctc tcatcatgcg gcaaatggaa tatcatgcaa tgaagcgcaa
     5701 aatcgcagac gtttgcgctc catcatggaa aacagtgggt ttgaagcata tagcctcgaa
     5761 tggtggcact atgtattaag agacgaacca taccccaata gctattttga tttccccgtt
     5821 aaataaactt ttaaccgttg cacggacaaa ctatataagc taactctttc ggcaggaaac
     5881 ccgacgtatg taactggttc ttagggaatt tatatatagt agatagtatt gaagatgtaa
     5941 ggcagagggt tctgttgcaa agttttaaat aaagaataaa atccc
//
LOCUS       DACDSY010000402         2611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.guided.225, whole genome shotgun sequence.
ACCESSION   DACDSY010000402 DACDSY010000000
VERSION     DACDSY010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.guided.225"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            22..1500
                     /gene="msr"
                     /locus_tag="IWK44_003169"
     CDS             22..1500
                     /gene="msr"
                     /locus_tag="IWK44_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9551589.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1547..1993
                     /locus_tag="IWK44_003170"
     CDS             1547..1993
                     /locus_tag="IWK44_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9551590.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2192..>2611)
                     /locus_tag="IWK44_003171"
     CDS             complement(2192..>2611)
                     /locus_tag="IWK44_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9551591.1"
                     /translation="HHSQIYTTLAKLDQQGYVTVLDKDEHSQKKIYHLTDEGRALVEE
                     WIKEETPAPTQKDEFLAKIYAFSTLDKTTATGLLFTREQQLNKIMQKNQKKLNDLSLD
                     KKQEFGRYVVIQRRIMLCQQELQWCQQVREQMQQFFE"
BASE COUNT          823 a          453 c          553 g          782 t
ORIGIN      
        1 tgaaatagga gtgtaaaaca tatggaaaat ttagcagtaa atataacaaa tctacaagtc
       61 agctttggca accagctaga actatctatt gattctcttc gtgtctatca gcaagatcgt
      121 atagggatca taggagaaaa tggtgtcggt aaatcgactt tgctcaaact aatagccggt
      181 gaactttttc ccgatcatgg gaaaatccaa acagagatca ccttcaacta cctgcctcaa
      241 ttaacctatc tcgctgaagc aaaggaccta aatttggaat tagccagtca tttccagtta
      301 agactggaag aaacttcaga gcggaaatgg agcggagggg aagaacgaaa gatcgagttg
      361 atacgtcttc tttcttctta tgaacaaggg atgcttctag acgagccaac aacccatcta
      421 gatagaaaaa gtattgatcg actgattgaa gagcttcgtt attattatgg tacgctggtt
      481 tttgttagtc atgatcgcta ttttctagat gagctagcat cgaaaatctg ggaagtaaaa
      541 gacggagaaa tccgagagtt ttcggggaat tatagtgcct atctcactca aaaggaattg
      601 gagaaaaaga ctcagctacg agaagcagag tcgatcatga aagagaaaaa acgattggaa
      661 aaatcgatcc aagaaaagaa aaaacaagcg gaaaagttgg aaaaagtgtc cagtaaaaag
      721 aaaaagcaac aaatcagact ggatcggttg tcttcctcta aacaaaaaga cagtgtacaa
      781 aaagccatcc aaaagaatgc gaaaacatta gagagaagac tccaaaaaat aggagaaaca
      841 accaagccgc agcagatgaa acaaatccgt tttccagtac caaaatctct tgaactccac
      901 agccgctatc ccatcatggg acaaaatgtc caattggaaa gaagtgggag aacattactg
      961 gtaaatggtg attttcagtt ttctttaggt aaaaaaatcg cgattgtcgg tgaaaatggt
     1021 tcaggtaaga caaccttatt agaacatatc cgcaaacaag gagaaggaat ccttctctct
     1081 ccgaaagtaa gctttcaagt atatcagcaa aagggttatc aaatgacatc tgaagaatcc
     1141 atcattcgtt ttgtcatgag acaaacagag ttttcagaat cgcttgtccg tagtttgctg
     1201 aatcacttag ggtttgctca ggaaactttg acgaaaccgt tatgtacatt aagtggggga
     1261 gaagcgaccc gtctgacgat tgctttgctt tttactaagc caagtaatgt gttgctgtta
     1321 gatgaaccga ctaattttat tgatatggca acgatcgaag ctttagagaa gctaatgcaa
     1381 atatatccgg gaacgatttt gtttacttct catgattctt actttgtcga gcgtacggct
     1441 gatgaagttt atgaaataaa agggcagaaa ataaaaaaag tacttacgag aaatttttaa
     1501 atgtgctacg atgattttcg tgacaaattt aaaggagtgg tacagattgc ctacaaataa
     1561 aaaagaagga ataattttta caacaatgat gtgttttttg atggtcttgg ggatgagcgc
     1621 gtataattta tggctgcatc aagatcttca tttatcaagt ttgttgattg gtctagtgcc
     1681 tggattcgtg gtggctttca ttttggatgt cttcatcatc ggtgtgctgg cgaaaaaaat
     1741 tgcctttaaa ctaccagtaa atccagaaag caaggtccaa atgatcttaa ctatttcatg
     1801 cttgatggtt ttaggaatgg tgacatgtat gtctttattc ggtgtgatca tggaacaagg
     1861 aatacctgat caattattct ctgcttatat gaatgcttgg aaaatgaatg tgattgccgc
     1921 tttaccgttg cagttgatca tcgttggccc gctttcaaga agtgtgctaa aagcaattca
     1981 aagtaatgaa tgatggaaca ctgttatttg aaaaaaggcc ttggcatgct ttttgtcata
     2041 gtctcttgaa tgcagttcta gaaaaatagt gatccgaacg ataggttttg gcctgtcgtt
     2101 cggatcgctt tttcttttcc aactgtattg ctcttttatt gaagataatt tgctcttcaa
     2161 tagatatttc tagaaaggtt ctgtttatcc cttactcaaa aaactgctgc atttgttctc
     2221 ttacttgctg acaccattgg agttcctgct gacaaagcat aattctgcgc tggatcacaa
     2281 cataacgtcc aaattcttgt ttcttgtcta gcgagaggtc attcaatttt ttttgatttt
     2341 tttgcatgat tttgttcagc tgttgttctc tagtgaatag cagtccagta gctgttgttt
     2401 tatcgagcgt cgaaaaagca tagatttttg ctagaaattc atctttttgc gtaggagcgg
     2461 gagtttcctc tttgatccat tcctctacaa gagcacgtcc ttcatcagtc aagtggtaga
     2521 tctttttttg agagtgctcg tctttgtcca aaacggtcac ataaccctgc tggtctaact
     2581 tggcaagcgt tgtatagatc tgactgtgat g
//
LOCUS       DACDSY010000403         2234 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.guided.240, whole genome shotgun sequence.
ACCESSION   DACDSY010000403 DACDSY010000000
VERSION     DACDSY010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.guided.240"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            347..844
                     /gene="dfrG"
                     /locus_tag="IWK44_003172"
     CDS             347..844
                     /gene="dfrG"
                     /locus_tag="IWK44_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9551592.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(916..>2234)
                     /locus_tag="IWK44_003173"
     CDS             complement(916..>2234)
                     /locus_tag="IWK44_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9551593.1"
                     /translation="NTILPMFAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLE
                     FLERYETQPLQTKKFNEMWLNTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSA
                     EVLSRYVFRSDVAYDWNISMDELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSAND
                     SENRNAYLHELGKITNRSRFVDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSI
                     RNAFYRVFTKELRLSDAHHFICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFL
                     RTLASHYLTELLTSHQFHEEAISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLS
                     ALEPNEIAKMLLNVNDHSTNSFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQ
                     FAITILRTYYNFCRPFKSADKKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT          731 a          357 c          391 g          755 t
ORIGIN      
        1 tttttctttt tctgatactc taaccgaatc tcgtaaatgt agtactccac tataaaggcg
       61 tatatatgca agtaagtagt tcagttttgg agtacaaaat gacaaaatgt acttcaaaat
      121 ggactacttt tttcatttta acttacatac caaattacat acaaaatgat ttataaaatg
      181 caattgaaaa tgataattga atgaaaattt gtgattttgt ggtattatga taatataact
      241 tatagaaaaa attgcttatt caactaacgg gcaggataat gagaaaaggt tttttgatag
      301 aaataagaaa ttagatggta aattagttaa aaaggctgtg ttataaatga aagtttcttt
      361 gattgctgcg atggataaga atagagtgat tggcaaagag aatgacattc cttggaggat
      421 tcccaaggac tgggaatatg ttaaaaatac tacaaaggga catccgataa tattaggtag
      481 gaagaacctt gaatcaatcg gaagagcctt acctgacaga agaaatatta ttctgacgag
      541 agataagggg tttaccttta atggttgtga aattgttcat tcaatagaag atgtttttga
      601 gttatgtaaa aacgaagaag aaatttttat tttcggagga gaacagattt ataatttgtt
      661 tttcccttat gttgagaaaa tgtacatcac aaaaatacat catgaattcg aaggagatac
      721 tttttttcca gaagtgaatt atgaggaatg gaatgaggta tttgcccaaa aagggataaa
      781 gaatgataaa aatccgtata actactattt tcatgtatat gaaagaaaaa acttattgag
      841 ttaacgggtg cgttaattga agaagaacag taaagataaa gcacaagagc ttgggattac
      901 tccaagctct ttttgtcatt tgaaataaat tatatctttc caatcaaatt gtttatctgt
      961 aatccctaat ctttgagcag gtgtcaaaac ctttttatcc gctgatttaa aagggcgaca
     1021 aaagttgtag taagttctta gaattgtaat cgcaaattga gcatacttcg gattgaagtt
     1081 agcgtaaata tagctcttct tatcaccacg agctgttgtt aatggtcttt caagagatga
     1141 tatataacgt cttatctgtt gaataaagct attggtcgaa tggtcattaa catttaatag
     1201 catctttgcg atttcgtttg gttccagagc agataaatcc gttctacaat caactgaata
     1261 aaatccttta tccttagtgg ctaatggatg tttgataggg ttatcggcat atttgcgata
     1321 tctttctcca tctttactaa tagcctcttc gtggaattga tgacttgtta gtaattcagt
     1381 tagataatga gatgccaaag tccgtagaaa ctttgttttg aatcctctga tatctcccca
     1441 atcgagcagt tctgcttttg cttgtccgaa ttcttttaag cattgttttc tgcttttcgt
     1501 cttgttaact tggcaaataa aatgatgtgc atcgctcaac cttaattctt tcgtgaatac
     1561 tctataaaag gcgtttctaa tagagaagtc atcatcacta atcattcgcc attcgtcggc
     1621 attaaccatt tgtttaatta accaatagtg agcagttgta gtataaggag catcaacatt
     1681 taaaccatcg acaaatctac tcctattagt aattttccct aattcgtgaa gataagcatt
     1741 cctattttcg ctatcgttag cggatggttc ttgtggataa taagaccaat ctaacctatc
     1801 atttttacga ctaaaagtat tgagatggtc ttctttgaat tttcttgtat cttcatttaa
     1861 ttcatccatt gaaatattcc aatcataggc aacatcagaa cggaacacat atcttgataa
     1921 gacctcagca gatacaacta tatacgtttg catattaagg tcttcaaacc ccgtatattt
     1981 ctttgaacct tgtcctttct tacggacgtt atttaggtag tagtgcatct tgtccgtatt
     2041 aagccacatc tcgttaaatt tctttgtttg aaggggttgt gtctcatatc gttctaaaaa
     2101 ctccaaacaa cgcctataca gccactccag cttatgatag taggttccaa ctccaattcc
     2161 taaaatatca caagtacgac tgactgaaac ccttccaaca accatcttag cgaacattgg
     2221 aagaatggtg ttct
//
LOCUS       DACDSY010000404         1504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.guided.284, whole genome shotgun sequence.
ACCESSION   DACDSY010000404 DACDSY010000000
VERSION     DACDSY010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.guided.284"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            262..373
                     /locus_tag="IWK44_003174"
                     /pseudo
     CDS             262..373
                     /locus_tag="IWK44_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="IWK44_003175"
     CDS             394..477
                     /locus_tag="IWK44_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9551594.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="IWK44_003176"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="IWK44_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9551595.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DACDSY010000405         1461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.guided.286, whole genome shotgun sequence.
ACCESSION   DACDSY010000405 DACDSY010000000
VERSION     DACDSY010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.guided.286"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            50..1090
                     /locus_tag="IWK44_003177"
     CDS             50..1090
                     /locus_tag="IWK44_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9551596.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1188..1376
                     /locus_tag="IWK44_003178"
     CDS             1188..1376
                     /locus_tag="IWK44_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9551597.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
BASE COUNT          498 a          223 c          281 g          459 t
ORIGIN      
        1 acgagattcg gttagagtat cagaaaaaga aaaaataaaa gttacagaaa tgtatacttc
       61 aataaatggt gaattatgta agattgatag agcttattct ggagaaattg ttattttgca
      121 aaatgagttt ttgaagttaa atagtgttct tggagataca aaactattgc cacagagaaa
      181 aaagattgaa aatccgcacc ctctactaca aacaactgtt gaaccgagta aacctgaaca
      241 gagagaaatg ttgcttgatg cccttttgga aatctcagat agtgatccgc ttctacgata
      301 ttacgtggat tctacgacac atgaaattat actttctttc ttagggaaag tacaaatgga
      361 agtgattagt gcactgttgc aagaaaagta tcatgtggag atagaactaa aagagcctac
      421 agtcatttat atggagagac cgttaaaaaa tgcagaatat accattcaca tcgaagtgcc
      481 gccaaatcct ttctgggctt ccattggttt atctgtatca ccgcttccgt tgggaagtgg
      541 aatgcagtat gagagctcgg tttctcttgg atacttaaat caatcatttc aaaatgcagt
      601 tatggaaggg atacgctatg gttgcgaaca aggattatat ggttggaatg tgacggattg
      661 taaaatctgt tttaagtacg gtttatacta tagccctgtt agtactccag cagattttcg
      721 gatgcttact cctattgtac tggagcaagc ctttagaaaa gctggaacag aattgttaga
      781 gccatatctt agttttaaag tttatgcacc acaggaatat ctttcacggg catataacga
      841 tgctcccaaa tattgtgcaa atatcgtaaa tactcaactg aaaaataatg aggtcattat
      901 tattggagaa attcctgctc gatgtattca agattatcgc aatgatttaa ctttttttac
      961 aaatgggctt agtgtttgtt tagcagagct aaaaggatat caggttacca ctggcgaacc
     1021 tgtttgccag acccgtcgtc taaatagtcg gatagataaa gtaagatata tgttcaataa
     1081 aataacttag tgcgttttat gttgttatat aaatatggtt tcttattaaa taagatgaaa
     1141 tattctttaa tatagatttg aattaaagtg gaaaggagga gattgttatt ataaactaca
     1201 agtggatatt gtgtcctatt tgtggaaata aaacaagact acgaatacga gtggatacta
     1261 tacttaaaaa tttcccttta tactgcccca aatgtaagaa cgaaacttta attaatgttc
     1321 aaaaaatgaa tataataaca atcaaagagc cagacgccaa gacgcagagc cgataatttg
     1381 agaaatgaaa ctctcatctt atcggctctt tttgtttatc tgaattttac tgactagcct
     1441 tcaatatttc ttgcttcatt g
//
LOCUS       DACDSY010000406         1372 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.guided.297, whole genome shotgun sequence.
ACCESSION   DACDSY010000406 DACDSY010000000
VERSION     DACDSY010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.guided.297"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            8..>1372
                     /gene="aph(2'')-I"
                     /locus_tag="IWK44_003179"
     CDS             8..>1372
                     /gene="aph(2'')-I"
                     /locus_tag="IWK44_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAP9551598.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          575 a          103 c          235 g          459 t
ORIGIN      
        1 taaataaatg aatatagttg aaaatgaaat atgtataaga actttaatag atgatgattt
       61 tcctttgatg ttaaaatggt taactgatga aagagtatta gaattttatg atggtagaga
      121 taaaaaatat acattagaat cattaaaaaa acattataca gagccttggg aagatgaagt
      181 ttttagagta attattgaat ataacaatgt tcctattgga tatggacaaa tatataaaat
      241 gtatgatgag ttatatactg attatcatta tccaaaaact gatgagatag tctatggtat
      301 ggatcaattt ataggagagc caaattattg gagtaaagga attggtacaa gatatattaa
      361 attgattttt gaatttttga aaaaagaaag aaatgctaat gcagttattt tagaccctca
      421 taaaaataat ccaagagcaa taagggcata ccaaaaatct ggttttagaa ttattgaaga
      481 tttgccagaa catgaattac acgagggcaa aaaagaagat tgttatttaa tggaatatag
      541 atatgatgat aatgccacaa atgttaaggc aatgaaatat ttaattgagc attactttga
      601 taatttcaaa gtagatagta ttgaaataat cggtagtggt tatgatagtg tggcatattt
      661 agttaataat gaatacattt ttaaaacaaa atttagtact aataagaaaa aaggttatgc
      721 aaaagaaaaa gcaatatata attttttaaa tacaaattta gaaactaatg taaaaattcc
      781 taatattgaa tattcgtata ttagtgatga attatctata ctaggttata aagaaattaa
      841 aggaactttt ttaacaccag aaatttattc tactatgtca gaagaagaac aaaatttgtt
      901 aaaacgagat attgccagtt ttttaagaca aatgcacggt ttagattata cagatattag
      961 tgaatgtact attgataata aacaaaatgt attagaagag tatatattgt tgcgtgaaac
     1021 tatttataat gatttaactg atatagaaaa agattatata gaaagtttta tggaaagact
     1081 aaatgcaaca acagtttttg agggtaaaaa gtgtttatgc cataatgatt ttagttgtaa
     1141 tcatctattg ttagatggca ataatagatt aactggaata attgattttg gagattctgg
     1201 aattatagat gaatattgtg attttatata cttacttgaa gatagtgaag aagaaatagg
     1261 aacaaatttt ggagaagata tattaagaat gtatggaaat atagatattg agaaagcaaa
     1321 agaatatcaa gatatagttg aagaatatta tcctattgaa actattgttt at
//
LOCUS       DACDSY010000407         1349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.guided.303, whole genome shotgun sequence.
ACCESSION   DACDSY010000407 DACDSY010000000
VERSION     DACDSY010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.guided.303"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            46..297
                     /locus_tag="IWK44_003180"
                     /pseudo
     CDS             46..297
                     /locus_tag="IWK44_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IWK44_003181"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IWK44_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9551599.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DACDSY010000408         1277 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.guided.313, whole genome shotgun sequence.
ACCESSION   DACDSY010000408 DACDSY010000000
VERSION     DACDSY010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.guided.313"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            366..453
                     /locus_tag="IWK44_003182"
                     /pseudo
     CDS             366..453
                     /locus_tag="IWK44_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            511..1242
                     /gene="erm(A)"
                     /locus_tag="IWK44_003183"
     CDS             511..1242
                     /gene="erm(A)"
                     /locus_tag="IWK44_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP9551600.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
BASE COUNT          479 a          203 c          197 g          398 t
ORIGIN      
        1 aaaaagatcc cccattccta ctttatcgac attctttcat tacttaccac tttagatgtt
       61 ttttcgttgg ggataaaact tccctttaga caattttatc caaagacaat acaacagtgc
      121 aactttatta aagtcactgt cctttatcgc agcctttact ttttagtaaa gacagtggct
      181 tctcttatca agtttcaaaa catattattt tgaagaaaac gtccatctga agtgtcaagt
      241 gcaaaattac atataaaggt ttattctaaa atgaaaagat gatacaatca tattcagtta
      301 cataaggagg tttcaattat gtgcaccagt atcgcagtag tagaaattac tttatctcat
      361 tcataatgaa aaaaatggaa aggagataaa agtatgggta ctttttctat atttgttatt
      421 aataaagttc gttatcaacc aaatcaaaat taattggtta taatgaacgc ttaatgtcag
      481 ttcattataa ccagtaagga gaaggttata atgaaccaga aaaaccctaa agacacgcaa
      541 aattttatta cttctaaaaa gcatgtaaaa gaaatattga atcacacgaa tatcagtaaa
      601 caagacaacg taatagaaat cggatcagga aaaggacatt ttaccaaaga gctagtcaaa
      661 atgagtcgat cagttactgc tatagaaatt gatggaggct tatgtcaagt gactaaagaa
      721 gcggtaaacc cctctgagaa tataaaagtg attcaaacgg atattctaaa attttccttc
      781 ccaaaacata taaactataa gatatatggt aatattcctt ataacatcag tacggatatt
      841 gtcaaaagaa ttacctttga aagtcaggct aaatatagct atcttatcgt tgagaaggga
      901 tttgcgaaaa gattgcaaaa tctgcaacga gctttgggtt tactattaat ggtggagatg
      961 gatataaaaa tgctcaaaaa agtaccacca ctatattttc atcctaagcc aagtgtagac
     1021 tctgtattga ttgttcttga acgacatcaa ccattgattt caaagaagga ctacaaaaag
     1081 tatcgatctt ttgtttataa gtgggtaaac cgtgaatatc gtgttctttt cactaaaaac
     1141 caattccgac aggctttgaa gcatgcaaat gtcactaata ttaataaact atcgaaggaa
     1201 caatttcttt ctattttcaa tagttacaaa ttgtttcact aaattaaagt aataaagcgt
     1261 tctctaattt cacaaga
//
LOCUS       DACDSY010000409         1148 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.guided.322, whole genome shotgun sequence.
ACCESSION   DACDSY010000409 DACDSY010000000
VERSION     DACDSY010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.guided.322"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..171
                     /locus_tag="IWK44_003184"
                     /pseudo
     CDS             1..171
                     /locus_tag="IWK44_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708502.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            202..1077
                     /locus_tag="IWK44_003185"
                     /note="aph(2'')-Ia"
     CDS             202..1077
                     /locus_tag="IWK44_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAP9551601.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
BASE COUNT          491 a           89 c          191 g          377 t
ORIGIN      
        1 gaatttttga aaaaagaaag aaatgctaat gcagttattt tagaccctca taaaaataat
       61 ccaagagcaa taagggcata ccaaaaatct ggttttagaa ttattgaaga tttgccagaa
      121 catgaattac acgagggcaa aaaagaagat tgttatttaa tggaatatag atatgatgat
      181 aatgccacaa atgttaaggc aatgaaatat ttaattgagc attactttga taatttcaaa
      241 gtagatagta ttgaaataat cggtagtggt tatgatagtg tggcatattt agttaataat
      301 gaatacattt ttaaaacaaa atttagtact aataagaaaa aaggttatgc aaaagaaaaa
      361 gcaatatata attttttaaa tacaaattta gaaactaatg taaaaattcc taatattgaa
      421 tattcgtata ttagtgatga attatctata ctaggttata aagaaattaa aggaactttt
      481 ttaacaccag aaatttattc tactatgtca gaagaagaac aaaatttgtt aaaacgagat
      541 attgccagtt ttttaagaca aatgcacggt ttagattata cagatattag tgaatgtact
      601 attgataata aacaaaatgt attagaagag tatatattgt tgcgtgaaac tatttataat
      661 gatttaactg atatagaaaa agattatata gaaagtttta tggaaagact aaatgcaaca
      721 acagtttttg agggtaaaaa gtgtttatgc cataatgatt ttagttgtaa tcatctattg
      781 ttagatggca ataatagatt aactggaata attgattttg gagattctgg aattatagat
      841 gaatattgtg attttatata cttacttgaa gatagtgaag aagaaatagg aacaaatttt
      901 ggagaagata tattaagaat gtatggaaat atagatattg agaaagcaaa agaatatcaa
      961 gatatagttg aagaatatta tcctattgaa actattgttt atggaattaa aaatattaaa
     1021 caggaattta tcgaaaatgg tagaaaagaa atttataaaa ggacttataa agattgatta
     1081 tataatatat gaaaagctat tataaaagac ggttctgttg caaagtttta aatctactat
     1141 caaataag
//
LOCUS       DACDSY010000410         1119 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.guided.325, whole genome shotgun sequence.
ACCESSION   DACDSY010000410 DACDSY010000000
VERSION     DACDSY010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.guided.325"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            182..>1119
                     /gene="vanY"
                     /locus_tag="IWK44_003186"
     CDS             182..>1119
                     /gene="vanY"
                     /locus_tag="IWK44_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9551602.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          404 a          155 c          226 g          334 t
ORIGIN      
        1 atgggtatca cttatgaaaa ttcatctaca ttggtgataa tagtaaatcc agtagggcga
       61 aataattgac tgtaatttac ggggcaaaac ggcacaatct caaacgagat tgtgccgttt
      121 aaggggaaga ttctagaaat atttcatact tccaactata tagttaagga ggagactgaa
      181 aatgaagaag ttgttttttt tattgttatt gttattctta atatacttag gttatgacta
      241 cgttaatgaa gcactgtttt ctcaggaaaa agtcgaattt caaaattatg atcaaaatcc
      301 caaagaacat ttagaaaata gtgggacttc tgaaaatacc caagagaaaa caattacaga
      361 agaacaggtt tatcaaggaa atctgctatt aatcaatagt aaatatcctg ttcgccaaga
      421 aagtgtgaag tcagatatcg tgaatttatc taaacatgac gaattaataa atggatacgg
      481 gttgcttgat agtaatattt atatgtcaaa agaaatagca caaaaatttt cagagatggt
      541 caatgatgct gtaaagggtg gcgttagtca ttttattatt aatagtggct atcgagactt
      601 tgatgagcaa agtgtgcttt accaagaaat gggggctgag tatgccttac cagcaggtta
      661 tagtgagcat aattcaggtt tatcactaga tgtaggatca agcttgacga aaatggaacg
      721 agcccctgaa ggaaagtgga tagaagaaaa tgcttggaaa tacgggttca ttttacgtta
      781 tccagaggac aaaacagagt taacaggaat tcaatatgaa ccatggcata ttcgctatgt
      841 tggtttacca catagtgcga ttatgaaaga aaagaatttc gttctcgagg aatatatgga
      901 ttacctaaaa gaagaaaaaa ccatttctgt tagtgtaaat ggggaaaaat atgagatctt
      961 ttattatcct gttactaaaa ataccaccat tcatgtgccg actaatcttc gttatgagat
     1021 atcaggaaac aatatagacg gtgtaattgt gacagtgttt cccggatcaa cacatactaa
     1081 ttggttctgt tgcaaagttt taaatctact atcaaataa
//
LOCUS       DACDSY010000411         1001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.guided.335, whole genome shotgun sequence.
ACCESSION   DACDSY010000411 DACDSY010000000
VERSION     DACDSY010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.guided.335"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            89..871
                     /locus_tag="IWK44_003187"
     CDS             89..871
                     /locus_tag="IWK44_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP9551603.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
BASE COUNT          346 a          141 c          205 g          309 t
ORIGIN      
        1 gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat aaactttata
       61 tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa aaataccaaa
      121 tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa tagttggagt
      181 atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg tagatgttct
      241 agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag aaagactaat
      301 gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag ttacggttat
      361 aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta tatacggtga
      421 gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg atcctgattt
      481 ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc ctgattcttc
      541 aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg attctttgcc
      601 agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc tagctcgaat
      661 gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag aatgggctat
      721 acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag gctatcgggg
      781 agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg ttaagtatat
      841 gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa caggtgctta
      901 cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga ataaagcgtc
      961 ctcttgtgaa attagagaac gctttattac tttaatttag t
//
LOCUS       DACDSY010000412          780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20807_2015
            SAMN12639619-rid9882763.guided.357, whole genome shotgun sequence.
ACCESSION   DACDSY010000412 DACDSY010000000
VERSION     DACDSY010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639619
            Sequence Read Archive: SRR10030743
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742048.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742048.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:16:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,188
            CDSs (total)                      :: 3,124
            Genes (coding)                    :: 3,045
            CDSs (with protein)               :: 3,045
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 30 of 79
            Pseudo Genes (incomplete)         :: 39 of 79
            Pseudo Genes (internal stop)      :: 20 of 79
            Pseudo Genes (multiple problems)  :: 8 of 79
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639619-rid9882763.guided.357"
                     /isolate="E20807_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            167..715
                     /gene="aac(6')"
                     /locus_tag="IWK44_003188"
     CDS             167..715
                     /gene="aac(6')"
                     /locus_tag="IWK44_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9551604.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          281 a          114 c          170 g          215 t
ORIGIN      
        1 tctttcctat tttgaataaa gtgtttctgt ataatttttt tattttataa gtaagataaa
       61 taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga acatttcgtt
      121 ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga taatcagtga
      181 atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga gactgacttg
      241 gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga atccagaacg
      301 aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg caatccctca
      361 atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc gacgaaagaa
      421 ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca gaggaggaat
      481 cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc aaacggacct
      541 gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac atccgtatga
      601 attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa atggctggga
      661 caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc aataataaaa
      721 agaggttgtg acagaagtaa tcagcttcaa gaaataagaa ggaatttacg aaaattactt
//
